LOCUS       DASUTZ010000001        16575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_907183, whole genome shotgun sequence.
ACCESSION   DASUTZ010000001 DASUTZ010000000
VERSION     DASUTZ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 16575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16575
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907183"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VF097_00005"
     CDS             <1..811
                     /locus_tag="VF097_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010070205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEX6261212.1"
                     /translation="GSGIVEVCAKADCEVVTVEVDDQAMDRGRQRFEKSLGTAVDRGK
                     LEEGARDQILGRITSTTDLDEAADADLVIEAVTENLDEKLLVFRRLDEITRDEVILAT
                     NTSSLPIVKLAAATKRPDRVVGMHFFNPPPIMKLLEIVRALTTSDETLGFAKGMGERL
                     GKTTVLSKDRAGFIVNMLLIPYLNNAVRMLEEGFASREDIDTAIQLGLGHPMGPLTLL
                     DFIGLDTAVYVSEVLFEEFRDPDYSAPPLLRRMVDAGWHGRKTGKGFYDYD"
     gene            816..1655
                     /locus_tag="VF097_00010"
     CDS             816..1655
                     /locus_tag="VF097_00010"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6261213.1"
                     /translation="MPKAIDLHVHLPTDEWLEQAIGPHLEATERYFRKSPPRKTLDEV
                     AEEYAALDVLGVVLDWDDESVTGRGWLGNDFMASLSERYPGVLMGFGSVDPHREDAVA
                     EVDRVADLGLKGLKFHPTMQRFGPGDERFYPIYERMQERGLVALMHTGTCGIGAGTPG
                     AGGTKIRYSHPGFLDSVGADFPEVTWIAAHFGWPWYPECLAYALHKSNVWIELSGWAP
                     GYLPAEVRREARRRLNERTLFGSDYPFIPLDRWFSEFEDLGYDEDQKANILHRNAVRL
                     LGL"
     gene            1672..2445
                     /locus_tag="VF097_00015"
     CDS             1672..2445
                     /locus_tag="VF097_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6261214.1"
                     /translation="MAGEEDRFDVDETFDEDYLYFYAHLTPELSEEQADLIWRLLDIQ
                     PGMEVLDLACGHGRIANRLAVRGARVTGLDQTRVFLDLARGEATQMGVTVKYVEGDMR
                     AIPWTGTFDRVVSWFTSFGYFDDEGNRDVLRQAHGALKPGGRFLVDLNHRDWLIANML
                     EETVVRRGDDIMVDGHRFDPLTGRLHDDRLVIRDGRVRRFRFFTRLFTFTEVGGWLEA
                     EGFTGVGGYGEDGEALTLDHRRMIVTSRRSTGGARPSGG"
     gene            complement(2414..2680)
                     /locus_tag="VF097_00020"
     CDS             complement(2414..2680)
                     /locus_tag="VF097_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261215.1"
                     /translation="MHAIARDWRTAPLSPADRALCELAEKLTHRQHEMTPEDLEALRG
                     HGFDDHAIHDAVQVVGYFNYITRVADALGVEPEDFIPRWGGLRP"
     gene            complement(2756..3001)
                     /locus_tag="VF097_00025"
     CDS             complement(2756..3001)
                     /locus_tag="VF097_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase"
                     /protein_id="HEX6261216.1"
                     /translation="MPWIEQIPIEDADGLLKRQFEAALARAGRVWHIVHVMSLNPEAM
                     RDSIRFYATIMMGESPLTRVQRELLATVVSAEAQCFY"
     gene            complement(3001..4101)
                     /locus_tag="VF097_00030"
     CDS             complement(3001..4101)
                     /locus_tag="VF097_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX6261217.1"
                     /translation="MDLSEFRAQFPALTDHTWLNTATAPPAARPVAEELRRVQREWET
                     GEFAWTDWESEAYATRPLFARLIGADPDEVALASSVSYAAATVAASLSPRRIVVGARE
                     FRSNYFPWVALADRGFEIVEVPVNAEGVVTTDALLEGVTEGALVAVSEVQSSNGYRID
                     LPRLTRETQAQGGRVFVDLCQSLGCLRVDAGALGVDYAAVHGYKWMLGPRGAGWLYVR
                     RDHVDECRPITPGWKSPEDPYAEYYGGPLEYAKTARKLDVSLAWFSWPGARAALELLL
                     SLDAEAVEQRALELAQAFREGAAERGYDVCVEETPSQIVGMEVPDVEALQHRLREQRV
                     VAAVRGGFLRIGFHGFNDEGDVERGLAALDRA"
     gene            complement(4103..5023)
                     /locus_tag="VF097_00035"
     CDS             complement(4103..5023)
                     /locus_tag="VF097_00035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6261218.1"
                     /translation="MKVSLVLTVQDAAETMERFLDSVRAQTLQPDEIVVVDRGSADGT
                     PEILRAAGDVILLEAPGASIAKGRNLAIEVATHDLIAVSEADCVLSPHWLEHLAMALE
                     AGADVAMGYYRPIAHTLFETAAGAIAAPEPHEIDEVRFMPSSRSIAFRRDMFERTGGY
                     PEWLESGEDLYLDHRLRDLYADMRFVPEAVAHWRIRPTLGETFRQYFRYAEGDGRAGM
                     YPERHLLRFTAYGLLAYAAARRRRPLLAALALGGSFYAGRRVRRLTGMLPGRPAAQAA
                     ASALIPPMMAMIDVAKMAGYVSGLLEKARS"
     gene            complement(5049..5354)
                     /locus_tag="VF097_00040"
     CDS             complement(5049..5354)
                     /locus_tag="VF097_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6261219.1"
                     /translation="MSARLTPNSGPPLSREDLLARFAEAGLRPRWWGNAAGDEYGSHA
                     HEHHKVLYCLEGSITFHTEEGDLELRPGDRLDLDADTAHAATVGPNGVTCVEAYVEG"
     gene            complement(5351..5617)
                     /locus_tag="VF097_00045"
     CDS             complement(5351..5617)
                     /locus_tag="VF097_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEX6261220.1"
                     /translation="MNRRGRPAGPPVRWVRVAEYSDMESARLMAGLLESEGIEVTLDP
                     PDVTSYWDRGMARVLGYRIKIMVLEHRQVEAAEIVQEVEEGRHR"
     gene            5674..6783
                     /locus_tag="VF097_00050"
     CDS             5674..6783
                     /locus_tag="VF097_00050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6261221.1"
                     /translation="MTPSRDVRTGFERRDLPLVVVSAVALVSGGPLTILRQAVAAARA
                     SSDANFLFLVHDASSWEEGPNVRFASFPGARRSYLRRVLTEYLRFPSLSRRWRPDVWL
                     SLHDVTPPVVARRQAVYCHNPLPYWRPTFRDLRLHPWEVVRSFLYGWVYRTNARRNDP
                     VIGQLPWFTEFIGRYMRVPRERLVVVAPRSEATVEIEEVPPPAGTGLTCVYASLPRVF
                     KNFEEAIALCDQPGVRLTVTLSGDENRYARWVRERARGDVTFAGLMAHAEALAAMARA
                     DVVLFPSRLETFGLPVQEAIALGRTLVLPVRPWTVAVAGEYPRAHFYRTVEEGRAILA
                     ALRDGREPPGIRPTVPENDLPRLSGFGELYRFLLR"
     gene            6807..7940
                     /locus_tag="VF097_00055"
     CDS             6807..7940
                     /locus_tag="VF097_00055"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX6261222.1"
                     /translation="MRSPAVAFDVTPLAGFRTGIGLAVQETHDALVEAGAELVPYAIG
                     LRVRTDGLPGGTRVARLPTRGLVAAWGRADRPRIDRWVRGARVLHATNYLTPPSRLPT
                     LVTIHDCAFALFPETVSTVVLGFARVLRRAVARGAWVHCTTEAVAEEVEDLFGPGLRR
                     EGRIAVVPFGVPRLDASEALAGALEERLRGEPYVLALGTLEPRKNLARLVRAFGRIGG
                     EHRDLRLVVAGPDGPGRPAVDDAIASLEPSVRGRVVLAGAVGPGARRTLIERAAVLAY
                     PSVYEGFGFPMLEAMTLGVPVVGGAAGALEEVAGGAAHHVDPLDPDAIADGLRAVLEE
                     NDLRRTLIRRGGERAAQFTWDRTAKGLRETYSALAGSEARTSS"
     gene            complement(7898..8914)
                     /locus_tag="VF097_00060"
     CDS             complement(7898..8914)
                     /locus_tag="VF097_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX6261223.1"
                     /translation="MTRARVALFSRKPTPGVFSIEQVFGLIADRLPDRFEPIKRVASY
                     RSKGVLPRALAVLEARRRQGDLNHVVGDVNFLALLLPRDRTIVTIHDCEFMERASAVK
                     RWIYRWVWLELPARRAALVVVPTHGVREDLQRYTSVDPAKVRVIPMPVDPRFRPSPMR
                     GGPSEPVILQVGTRPNKNLERVIRALEGIPCRLIVLGELTQEQRGLLGRCAVRFEEHV
                     SLPHDEVPELYGESDLVVFASTKEGFGLPIAEAQASGRPVVAGDRPPLPEVAGEGACL
                     VDPYDVGSIRAGIERVLGDAPYRERLIAEGLRNVDRFEAGKIAEAHAALYDEVLASLP
                     ARAE"
     gene            complement(8911..9162)
                     /locus_tag="VF097_00065"
     CDS             complement(8911..9162)
                     /locus_tag="VF097_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261224.1"
                     /translation="MPRYELPPDLSAEEERIVLAAIERMLELERPTLSPWVVAGRAEG
                     LRIGGLHIRHQTADPWTSHAPLPFAPGGTQRLPGRGDAR"
     gene            complement(9165..10742)
                     /locus_tag="VF097_00070"
     CDS             complement(9165..10742)
                     /locus_tag="VF097_00070"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX6261225.1"
                     /translation="MAKRTEPAAEIEAKKLKELRRLNKKAEVAGGEEAIERQHARGKL
                     TARERIDLLLDPGSFVETDKLVKHRATDLGMARNRPYTDGVVTGWGTIDGRKVFVYSQ
                     DFTVFGGSLGEVHAEKICKVMDLAASVGAPVIGLNDSGGARIQEGASSLAGFGTIFDR
                     NVRFSGIIPQISAIMGPCAGGAVYSPAVTDFTYMVKETSHMFITGPDVIKTVTGEDVT
                     FEDLGGAMTHASRSGLSSFVAEDEEDCLQQIRYLLSFLPSNNMEDPPAYAPTDDPERR
                     VEDLNAIVPDSAKEPYDMKDVVRGVVDEGEFYEVFPLWAMNVVVGFARLDGLSVGIVA
                     NQPKVLAGTLDIESSEKAARFIRFCDAFNIPLVTFVDVPGFLPGAAQEYGGIIRRGAK
                     LLYAFTEATVPRMTVITRKAYGGAYVVMNSKHIRADVSFAWPTAEIAVMGSGGAVNII
                     HRREIKEADEPEATRAKLIERYEKSFLNPYRAAERGYVDDVIEPAETRIKLIRSLRML
                     HTKREQVPARKHGNIPL"
     gene            complement(10753..11175)
                     /locus_tag="VF097_00075"
     CDS             complement(10753..11175)
                     /locus_tag="VF097_00075"
                     /inference="COORDINATES: protein motif:HMM:NF015444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HEX6261226.1"
                     /translation="MRAYQSEDLTLLRLPPGTDIYEGVTGAAKELGIRAAAVAVIGAL
                     RSLEYGYYDQVIHEYTHLTHEGELEIASANGNVSIRDGEPFLHMHIVAGKPDGTTIGG
                     HLFGGELFVAEVALHLFRGEAPVRELDEEIGLPMWPTR"
     gene            11239..12165
                     /locus_tag="VF097_00080"
     CDS             11239..12165
                     /locus_tag="VF097_00080"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX6261227.1"
                     /translation="MELTPVQQRTLDELMRMQPLPVFPRELVEGLRADLEHRLRPLTD
                     NLRDGEGVWLSKSKLSDLHSRCEGLFLANHLGEGEFELGWQLARGALVHRAIAVSVNL
                     PDRSDAELVEAALAQLRRDDGAFDEYCVGLSDADLAALESEAVERVLLFRATFPPLQK
                     AWSPAVEPSLKATFAGDRVVLSARPDLLLGSDDREEPMRARRLVIELKTGGDRPEHDD
                     DLRFYALLATLRLGVPPFRVATVFLDDGTWRAHDVSADLLEAAVRRIGDGAIRAGAII
                     AGHEEPRLRPGPWCRWCPRAETCPESMFRADG"
     gene            complement(12531..12776)
                     /locus_tag="VF097_00085"
     CDS             complement(12531..12776)
                     /locus_tag="VF097_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261228.1"
                     /translation="MSELGSHAVTREELREELLEGLRGLEERTDTKLQLLEARMDTKL
                     ANLETRITRTFVRTFYVSNAAMVLAVATLAFGAARLA"
     gene            13262..14500
                     /gene="ccrA"
                     /locus_tag="VF097_00090"
     CDS             13262..14500
                     /gene="ccrA"
                     /locus_tag="VF097_00090"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017589235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HEX6261229.1"
                     /translation="MTKDIYEIGEIPPLGEAPKRMYAQVIRPERYGEPQDAFQIEEIP
                     VPEVGPDDALVLVMAAGINYNNVWAAQGIPIDVTKVHGKLGEPDDFHIGGSDASGIVY
                     AVGENVTNVSVGDHVVVHCGQWDREADDLPDPMLHPSFRIWGYESNWGSFAQFTKIQA
                     HQLLPKAPQLTWEAAAAPTLVGATAYRMLFGWPPHTVEEGDVVLIWGAAGGLGSMAIQ
                     LVAQAGGKPIGVVSSEDKFEFCRKLGAVGVINRKDFDHWGMPPHWTDGEAYGKWAEGA
                     RAFGKAMWDALGERRSPKIVFEHPGESTIPTSIFVADTGGMVVICAGTTGYNAVVDLR
                     YLWTRQKRFQGSHFANDEQSRAFNQMVIDGTVDPALSRTFSFDDIGTAHQLMSENAHP
                     DGNMAALVSAPSEGLTDVPA"
     gene            14497..15072
                     /locus_tag="VF097_00095"
     CDS             14497..15072
                     /locus_tag="VF097_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261230.1"
                     /translation="MRRALVLTFALVAAACGGEAEGEGDVATDVWVRDVCTTLTDWND
                     RVQSRTGTLQTDLQGLPQGDFQALKDLMVSYIDGVIEDTDQAVTSLEGAGAPAVEDGG
                     EASRLVVRGIGEVRTIFEQAREDIAALDASRPRRFAMALQDIGLRVQQGGQQVQATFQ
                     EVDQRGIGGEELDQAFDEEPACAPLAGGAGP"
     gene            complement(15069..15470)
                     /gene="mce"
                     /locus_tag="VF097_00100"
     CDS             complement(15069..15470)
                     /gene="mce"
                     /locus_tag="VF097_00100"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011437170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX6261231.1"
                     /translation="MLTDIDHVGIAVQDLDEAVERYRAAFGVEPTHRETVEDQGVDEV
                     MFKVGTSYIQLLGSRGPDTPVGRYLATRGEGIHHIAYRVNDVEEVLAHLMEQGHRLVD
                     ERGRPGSRGTMVAFVHPKALHGTLIELVQEA"
     gene            complement(15470..15838)
                     /locus_tag="VF097_00105"
     CDS             complement(15470..15838)
                     /locus_tag="VF097_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261232.1"
                     /translation="MARKKREPVPADAARHIGGLLRGVRKTCGYRAVREAAQAKGCPA
                     ALQTIYAYERGGLLPSLPQFLQLIDFYVGADGVSEEARLRAVAAIHAALSSPAYHVPQ
                     AQELAARLHPAVDLGRRRTR"
     gene            16013..>16575
                     /locus_tag="VF097_00110"
     CDS             16013..>16575
                     /locus_tag="VF097_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6261233.1"
                     /translation="MGEADRGGRAQEQAATNDVAAVLGQSTLIKAGRADVSRIRERTR
                     RRRLVRLALVIGLIDGYLWYRYASGNPIGLPSLPQDAWIWLPGLLLVALFAIVILLPM
                     GSGRSPAMVVRPEHIEVGLEELKGLDAQVDDVIRTLNVFLGYSTFRSRLGGNPRRGVL
                     FEGPPGTGKTFLAKAMAKQAGVPFLFVS"
BASE COUNT         2365 a         5799 c         5859 g         2552 t
ORIGIN      
        1 ggggtcgggc atcgtcgagg tctgcgccaa ggccgactgc gaggtcgtga cggtggaggt
       61 cgacgaccag gcgatggacc ggggccgtca gcgcttcgag aagtcgctgg gcaccgcggt
      121 ggaccggggc aagctcgagg aaggcgcccg cgaccagatc ctggggcgca tcacctcgac
      181 caccgatctc gacgaggcgg ccgacgccga tctcgtgatc gaggccgtca ccgagaacct
      241 ggacgagaag ctcctcgtct tccgcaggct cgacgagatc acccgcgacg aggtgatcct
      301 ggccacgaac acctcctccc tccccatcgt gaagctggcc gcggccacga agcgccccga
      361 ccgcgtcgtg gggatgcact tcttcaaccc gccgccgatc atgaagctgc tcgagatcgt
      421 ccgggcgctg acgacgtccg acgagactct cgggttcgcc aaggggatgg gtgagcggct
      481 ggggaagacg accgtcctct ccaaggatcg ggccgggttc atcgtcaaca tgctgctgat
      541 cccgtacctc aacaacgccg tgcggatgct ggaggagggg ttcgccagcc gcgaggacat
      601 cgacacggcg atccagctcg ggctcgggca ccccatgggc ccgctcaccc ttctcgactt
      661 catcgggctg gacacggctg tctacgtctc ggaggtgctg ttcgaggagt tccgcgaccc
      721 ggactactcg gccccaccgc tgctccgccg catggtggac gcggggtggc acggccggaa
      781 gaccggcaag ggcttctacg actacgactg agcccgtgcc caaggccatc gacctccacg
      841 tccacctgcc gaccgacgag tggctcgagc aggcgatcgg accgcacctc gaagccaccg
      901 agcgctactt ccggaagtct ccgcctcgaa agaccctgga cgaggtggcc gaggagtacg
      961 cggcgctcga cgtcctcggg gtggtcctcg actgggacga cgagtcggtc accgggcgcg
     1021 gatggctggg caacgacttc atggcctcgc tgagcgagcg gtacccgggc gtcttgatgg
     1081 ggttcggctc ggtggatccc caccgtgagg acgccgtggc cgaggtggac cgcgtggccg
     1141 acctcgggct gaaagggctg aagttccacc ccacgatgca gcggttcggc cccggcgacg
     1201 agcgcttcta cccgatctac gagcggatgc aggagcgggg cctcgtggcg ctgatgcaca
     1261 cggggacctg cggcatcggg gcggggaccc ccggggccgg gggcacgaag atccgctact
     1321 cgcacccggg gttcctcgac tcggtggggg ccgacttccc cgaggtgacc tggatcgccg
     1381 cgcacttcgg ctggccctgg tacccggagt gcctcgccta cgcgctccac aagtcgaacg
     1441 tgtggatcga gctgtcgggg tgggcgcccg gctacctgcc ggcggaggtt cggcgtgagg
     1501 cccgacgccg cctcaacgag cggaccctct tcggcagcga ctaccccttc atcccgctcg
     1561 accgctggtt ctcggagttc gaggacctcg gctacgacga ggaccagaag gcgaacatcc
     1621 tccaccgcaa cgcggtgcgc ctcctcggcc tctgaggagt accctcccgg catggcggga
     1681 gaggaggatc gcttcgatgt cgacgagacg ttcgacgagg actacctcta cttctacgcg
     1741 cacctcacgc cagagctctc ggaggagcag gccgatctga tctggcggct gctcgacatc
     1801 cagccaggga tggaggttct cgacctcgcc tgcggccacg gccggatcgc caaccgcctc
     1861 gcggtgcgcg gtgctcgcgt caccggtctc gaccagactc gcgtgttcct cgacctggcc
     1921 cggggcgagg ccacgcagat gggggttacc gtgaagtacg tcgagggcga catgcgggcc
     1981 atcccctgga ccggaacgtt cgaccgcgtc gtcagctggt tcacctcgtt cggctacttc
     2041 gacgacgagg ggaacagaga cgtcctgcgg caggcccacg gagcgttgaa gcccggtggc
     2101 cgattcctcg tcgatctcaa ccatcgggac tggctgatcg caaacatgct ggaggagacg
     2161 gtggtgcgac gcggggacga catcatggtc gatggccacc gcttcgatcc gctgacggga
     2221 cgtctgcatg acgatcgcct cgtgatccga gatgggaggg ttcgacgctt ccgcttcttc
     2281 acgaggttgt tcacgttcac ggaggtcggt gggtggctgg aggcggaggg tttcacgggc
     2341 gtaggcgggt acggtgagga cggcgaagcg ctcacgctcg atcaccgccg gatgatcgtg
     2401 acctccaggc gctctacggg cggagcccgc cccagcgggg gatgaagtct tcgggctcga
     2461 ctccgagggc gtcggccacc cgcgtgatgt agttgaagta gccgaccacc tgcacggcgt
     2521 cgtggatggc gtgatcgtcg aagccgtggc ctcgcagggc ctcgaggtcc tcgggagtca
     2581 tctcgtgctg gcggtgggtc agcttctccg ccagctcgca cagcgcccgg tccgccgggc
     2641 tcaggggggc cgtccgccaa tcccgcgcta tcgcgtgcac gaaggtgtcg gcctccgcct
     2701 catcccaccc ctccttggcg acctcgtcac ggaggtcgtg cgcgtgcgcc tgggttcagt
     2761 agaagcactg cgcctccgcc gagaccacgg tggccagcag ctcccgctgg acccgcgtga
     2821 gcggcgactc gcccatcatg atcgtggcgt agaagcggat gctgtcgcgc atcgcttccg
     2881 ggttgaggct catcacatgg acgatgtgcc atacgcggcc cgcgcgggcg agcgcggcct
     2941 cgaactgcct cttgagcagg ccgtccgcgt cctcgatcgg gatctgctcg atccagggca
     3001 tcaggctcgg tcgagggcgg ccaggcctcg ctcgacgtcg ccctcgtcgt tgaagccgtg
     3061 gaagccgatg cggaggaagc cgccgcgtac cgccgcgacc actcgctgct cgcgcaggcg
     3121 atgctggagg gcctccacgt cgggcacctc catccccacg atctgggagg gggtctcctc
     3181 gacgcacacg tcgtatccgc gctcggcggc cccctcgcgg aaggcttgcg cgagctccag
     3241 ggcccgctgc tccacggcct cggcgtccag cgacaggagg agctcgagcg ccgcgcgggc
     3301 cccgggccac gagaaccacg ccagcgagac gtcgagcttc cgggcggtct ttgcgtactc
     3361 gagcggcccg ccgtagtact cggcgtaggg atcctcgggc gacttccagc ccggcgtgat
     3421 gggccggcac tcgtccacgt ggtcacgtcg cacgtacagc caccccgccc ctcgcgggcc
     3481 cagcatccac ttgtagccgt ggaccgccgc gtagtccacc ccgagcgctc ccgcgtcgac
     3541 ccggaggcag cccagcgact ggcagagatc gacgaagacg cggcctccct gcgcctgggt
     3601 ctcccgggtg aggcggggga ggtcgatcct gtacccgttc gaggactgga cctcgctgac
     3661 cgcgaccagg gccccctcgg tcacgccttc gaggagcgcg tcggtcgtga cgacgccctc
     3721 ggcgttcacg gggacctcga cgatctcgaa cccgcggtcg gccagcgcca cccacgggaa
     3781 gtagttgctg cggaactccc tcgctccgac cacgatcctg cgaggcgaga gggaggcggc
     3841 cacggtggcc gcggcgtagg acacggagga ggccagcgcc acctcgtcgg gatcggcccc
     3901 gatgagccgg gcgaagagcg gacgcgtcgc gtaggcctcg gactcccagt ccgtccaggc
     3961 gaactcgccc gtctcccact cgcgctggac ccggcggagc tcctcggcca ccggccgggc
     4021 cgccggcgga gcggtggcag tgttgagcca ggtgtggtcg gtgagcgccg ggaactgggc
     4081 ccggaactcc gacaggtcca cctcagctcc tcgccttctc caggaggccg gagacgtagc
     4141 cggccatctt cgccacgtcg atcatggcca tcatcggcgg gatcagggcc gaggcggccg
     4201 cctgcgccgc gggccgcccg ggaagcatcc cggtcaggcg ccggacccgc ctccccgcgt
     4261 agaaggaacc tcccagcgcg agcgcggcca gcagcggccg cctgcgtctc gccgccgcgt
     4321 aggcgagcag gccgtacgcg gtgaagcgga ggaggtgccg ctccgggtac atccccgccc
     4381 gcccgtcgcc ctccgcgtac cggaagtact ggcggaaggt ctcgccgagc gtggggcgga
     4441 tccgccagtg cgcgacggcc tcgggcacga accgcatgtc ggcgtaaaga tcgcgcagcc
     4501 ggtggtcgag gtagaggtcc tccccggact cgagccattc cgggtacccg ccggtccgct
     4561 cgaacatgtc ccggcggaag gcgatcgacc gggacgaggg catgaagcgg acctcgtcga
     4621 tctcgtgggg ctcgggcgcg gcgatcgctc cggccgccgt ctcgaacagg gtgtgcgcga
     4681 tcggacggta gtagcccatg gccacgtcgg cgccggcctc cagcgccatc gccagatgct
     4741 cgagccagtg gggcgagagg acgcagtcgg cctcggagac ggcgatcagg tcgtgggtgg
     4801 ccacctcgat ggccaggttg cggcccttgg cgatgctcgc cccgggcgcc tcgagcagga
     4861 tgacgtcgcc ggcggcccgg aggatctcgg gggtgccgtc ggccgagccg cggtccacga
     4921 ccacgatctc gtcgggctgg agggtctgcg cccggaccga gtcgaggaag cgctccatcg
     4981 tctcggccgc gtcctggacg gtgaggacca ggctgacctt catgggggag agtgtagacg
     5041 aggccgggtc acccctcgac gtaggcctcg acgcacgtga ccccgttcgg gccgacggtg
     5101 gcagcgtgcg cggtgtcggc gtcgaggtcg agccggtcgc cgggccggag ctccaggtcg
     5161 ccctcctccg tgtggaaggt gatcgagccc tcgaggcagt acaggacctt gtggtgctcg
     5221 tgcgcgtgcg acccgtactc gtccccggcg gcgttgcccc accagcgagg ccggaggccc
     5281 gcctccgcga accgggccag gaggtcctcg cgggaaagcg gcgggccgct gttcggcgtg
     5341 agcctcgcgc tcaccggtgc cggccctcct cgacctcctg cacgatctcg gccgcctcga
     5401 cctgccggtg ctcgaggacc atgatcttga tccggtagcc gaggacccgc gccatccccc
     5461 gatcccagta gctcgtcacg tcgggcgggt cgagggtgac ctcgatcccc tccgattcga
     5521 ggaggccggc catgagccgc gccgactcca tgtccgaata ctcggcgaca cgcacccacc
     5581 tgacgggcgg gcccgccggc cgccctcggc ggttcacgcc gacccttccg ggatgcggag
     5641 ggccccgcgg tcgtgttgga tggcctcccg gagatgacgc cgagccgaga cgtccggacg
     5701 gggttcgagc ggcgagacct gccgctcgtc gtggtctcgg ccgtggcgct cgtctcgggc
     5761 ggccccctga cgatcctccg ccaggctgtg gccgcggccc gcgcctcgag cgatgcgaac
     5821 ttcctgttcc tcgtccacga tgccagctcc tgggaggagg gccccaacgt gcggttcgcg
     5881 tcgttcccgg gggcgcgacg atcgtacctc cggagggtcc tcaccgagta cctgcggttc
     5941 ccgagcctct cgcgccggtg gcgtcccgac gtgtggctct ccctccacga cgtcaccccg
     6001 ccggtcgtgg cccggcgtca ggccgtctac tgtcacaatc cgctcccgta ctggcgaccg
     6061 accttccgcg acctccgcct ccacccctgg gaggtcgtcc gctcgttcct ttacggatgg
     6121 gtgtaccgga cgaacgcccg gcgcaacgac cccgtgatcg ggcagctgcc gtggttcacc
     6181 gagttcatcg ggcggtacat gcgcgtgccc cgggagcggc tcgtggtcgt ggccccccgg
     6241 agcgaggcca cggtcgagat cgaggaggtc ccgccgccgg cgggcacggg gctgacgtgc
     6301 gtctacgcct cgctcccgag ggtgttcaag aacttcgagg aggcgatcgc gctgtgtgac
     6361 cagccggggg tccggctcac ggtcacgctg tcgggggacg agaaccgcta cgcccggtgg
     6421 gtgcgggagc gagcgcgtgg cgatgtgacc ttcgccggcc tgatggcgca cgcggaggcg
     6481 ctggccgcga tggcgcgggc cgatgtggtg ctcttcccgt cgcggctgga gacgttcggc
     6541 ctgccggtgc aggaggcgat cgctctgggg cggacgctcg tgcttccggt gcggccctgg
     6601 acggtggcgg tcgccggcga atacccgcgg gcgcacttct accgaaccgt ggaggagggc
     6661 cgggccatcc tcgccgccct ccgtgacggc cgggagccgc ccgggatccg gccgacggtt
     6721 cccgagaacg acctgcctcg gctctccggg ttcggggagc tgtaccgctt cctgctccgc
     6781 tgaggcgatg tggtaggaat cggcccatgc gctcgccggc cgtcgcgttc gacgtcacgc
     6841 cgctggccgg cttccggacc ggcatcggcc tcgcggtgca ggagacgcac gacgcgctgg
     6901 tcgaggccgg ggccgaactc gtcccgtacg cgatcggcct gcgggtgcgg accgatgggc
     6961 tgccgggcgg cacccgcgtg gcccggctgc cgacccgggg gctcgtcgcg gcctggggtc
     7021 gcgcggaccg tccccggatc gatcggtggg tgcggggagc gcgggtgctg cacgccacga
     7081 actacctgac gccgccgagc cgcctgccaa cgctggtgac gatccacgac tgcgccttcg
     7141 ccctgttccc cgagaccgtg tcgacggtcg tgctgggctt cgcccgggtg ctccggcgtg
     7201 cggtggcccg gggggcctgg gtccactgca cgaccgaagc ggtggcggag gaggtcgagg
     7261 acctgttcgg gccgggcctg cgacgcgagg ggaggatcgc ggtggtgccg ttcggggtcc
     7321 ccaggctcga cgcgtccgag gcacttgccg gggcgctgga ggagcggctg cggggcgagc
     7381 cgtacgtgct cgcgctgggc acgctcgagc cgaggaagaa cctggccagg ctcgtgcggg
     7441 cgttcggccg gatcgggggc gagcatcgcg acctccggct cgtggtggcc gggcccgacg
     7501 gccccggccg tccggcggtg gacgatgcca tcgcgagcct cgagccttcc gtacggggcc
     7561 gggtggtcct ggccggggcc gtcgggcccg gcgcacggcg cacgctgatc gagcgggccg
     7621 ccgtgctggc ctatccctcg gtctacgagg ggttcgggtt cccgatgctc gaggcgatga
     7681 cgctcggcgt gccggtggtg ggcggcgccg ccggcgccct cgaagaggtc gccggcggcg
     7741 ccgcccacca cgtcgacccc ctcgacccgg acgccatcgc ggacgggctc cgggccgtcc
     7801 tcgaggagaa cgacctccgg aggaccctga tccggcgggg cggggagcgc gcggcgcagt
     7861 tcacctggga ccggacggcc aagggcctgc gggagaccta ctcggccctg gccgggagcg
     7921 aggccaggac ctcgtcgtag agcgccgcgt gcgcctcggc gatcttcccc gcctcgaacc
     7981 ggtccacatt ccggaggccc tccgcgatca gccgctcccg gtacggagca tccccgagca
     8041 cccgctcgat ccccgcgcgg atggacccga cgtcgtacgg atccaccagg caggctcctt
     8101 cgccggccac ctccggcagc ggaggccgat ccccggccac caccggccga ccgctggcct
     8161 gcgcctccgc gatcggcagc ccaaacccct ccttcgtcga ggcgaacacc acgaggtcgc
     8221 tctccccgta cagctcgggg acctcgtcgt gggggagtga cacgtgctcc tcgaaccgca
     8281 ccgcgcagcg tcccagcagc ccccgctgct cctgggtcag ctccccgagg acgatcagcc
     8341 ggcacgggat cccctcgagc gcccggatca ctcgctccag gttcttgttg ggcctcgtgc
     8401 ccacttggag gatgacgggc tcgctcggcc ccccccgcat cggcgacggc ctgaaccgcg
     8461 ggtccaccgg catcgggatg acccggacct tcgccgggtc cacgctcgtg taccgctgga
     8521 ggtcctcccg caccccatgc gtgggcacca cgacgagcgc cgcccgccgc gccggcagct
     8581 ccagccacac ccaccggtag atccaccgct tcacggcgct cgcccgctcc atgaactcgc
     8641 agtcgtggat ggtgacgatc gtccgatcgc gtggcaggag cagggcgagg aagttcacgt
     8701 cgcccacgac gtggttgagg tcgccctgcc gccgccgcgc ctccagcacg gccagcgccc
     8761 gcggcagcac ccctttgctc cggtaggagg ccacccgctt gatgggctcg aaccggtcgg
     8821 gcagccgatc cgcgatcagg ccgaagacct gctcgatgct gaagacgccg ggcgtgggct
     8881 tccgggagaa gagggcgacc cgcgcccggg tcatctcgcg tcgccgcgcc ccgggagccg
     8941 ctgggtcccg ccgggggcga acggcagggg ggcgtgcgag gtccacgggt ccgcggtctg
     9001 gtggcggatg tggagccctc cgatgcgaag cccctccgcc cgcccggcca cgacccacgg
     9061 cgagagcgtg gggcgctcga gctcgagcat ccgctcgatc gccgcgagga cgatccgctc
     9121 ctcctcggcc gagagatcgg gcggcagctc gtagcggggc atggtcagag cgggatgttg
     9181 ccgtgcttgc gggcgggcac ctgctcgcgc ttcgtgtgca gcatgcggag cgaccggatc
     9241 agcttgatcc gtgtctctgc cggctcgatc acgtcgtcca cgtacccacg ctccgcggca
     9301 cggtaggggt tgaggaagct cttctcgtac cgctcgatca gcttggcccg cgtggcttcg
     9361 ggttcgtcgg cctccttgat ctcgcgccgg tggatgatgt tcaccgcgcc gcccgagccc
     9421 atcaccgcga tctcggcggt gggccaggcg aacgagacgt ccgcccggat gtgcttcgag
     9481 ttcatcacga cgtacgcgcc cccgtaggcc ttccgcgtga tcacggtcat ccgcggcacg
     9541 gtggcctcgg tgaaggcgta cagcagcttc gccccacgcc ggatgatccc gccgtactcc
     9601 tgcgccgccc ccggcaggaa ccccgggacg tccacgaagg tcaccagcgg gatgttgaac
     9661 gcgtcgcaga accggatgaa gcgcgccgcc ttctcgctcg actcgatgtc gagcgtgcct
     9721 gccaggacct tcggctggtt ggccacgatc ccgacgctca gtccgtcgag ccgcgcgaac
     9781 cccaccacga cgttcatcgc ccacagcggg aagacctcgt agaactcgcc ctcgtccacg
     9841 acgccacgca cgacgtcctt catgtcgtag ggctccttgg ccgaatccgg gacgatcgcg
     9901 ttcaggtcct ccacccgtcg ctccgggtcg tcggtggggg cgtaggcggg cgggtcctcc
     9961 atgttgttcg agggcaggaa cgagagcagg tagcggatct gctggaggca gtcctcctcg
    10021 tcctcggcca cgaagctgga caggcccgag cggctggcgt gggtcatcgc gccgcccaga
    10081 tcctcgaacg tgacgtcctc gcccgtgacg gtcttgatga catcggggcc cgtgatgaac
    10141 atgtgcgagg tctccttcac catgtaggtg aagtcggtga cggcgggcga gtagacggcg
    10201 ccgcccgcgc acggccccat gatcgcgctg atctggggga tgatgccgct gaagcgcacg
    10261 ttgcggtcga agatcgtccc gaaccccgcg agcgaggacg ctccttcctg gatccgggcc
    10321 ccgcccgagt cgttgagccc gatcaccggc gcgcccacgg acgccgcgag gtccatcacc
    10381 ttgcagatct tctcggcgtg aacctcgccc agcgagcccc cgaagaccgt gaagtcctgc
    10441 gagtaaacga agacctttcg gccgtcgatc gtgccccaac cggtcacgac gccatcggtg
    10501 taggggcggt tgcgcgccat ccccaggtcg gtggcccggt gcttgacgag cttgtcggtc
    10561 tccacgaagg agcccggatc gaggaggaga tcgatccgct cccgcgcggt gagcttgccg
    10621 cgggcgtgct gacgctcgat cgcctcctcg cccccggcca cctcagcctt cttgttgagg
    10681 cgacgcagct ccttcagctt cttggcctcg atctccgctg ccggttccgt tcgcttcgcc
    10741 atggccggga gtctaccggg tgggccacat cgggaggccg atctcctcgt cgagctcccg
    10801 gaccggcgcc tccccgcgaa agaggtggag cgcgacctcg gccacgaaca gctcgccccc
    10861 gaacagatgg ccgccgatgg tggttccgtc gggcttcccg gccacgatgt gcatgtggag
    10921 gaacggctcg ccgtcgcgga tcgagacgtt gccgttggcg gaggcgatct ccagctcgcc
    10981 ctcgtgcgtg aggtgggtgt actcgtggat cacctggtcg tagtagccgt actcgaggct
    11041 gcggagcgcc ccgatgaccg ccaccgcggc cgcccggatc cccagctcct tggcggcgcc
    11101 cgtcaccccc tcgtagatgt cggtgcccgg cgggaggcgc agcaaagtga ggtcctcgct
    11161 ctggtaggcg cgcatggtgc tcctttcggt ctcgttcctg gggcatccta tgtcggtggc
    11221 ggaccgtacc ctgagcgcat ggagctgacg ccggtccagc agcgcacgct cgacgagctc
    11281 atgcggatgc agccgctccc cgtgttcccg cgcgagctcg tcgaggggct gcgggccgat
    11341 ctcgagcatc gcctccggcc gctcaccgac aacctgcggg acggcgaggg cgtctggctg
    11401 agcaagtcga agctctcgga cctgcacagt aggtgcgagg gactgttttt ggctaatcac
    11461 ctgggggagg gggagttcga gctgggctgg cagctggcta ggggggcact cgtccaccgg
    11521 gcgatcgcgg tgtcggtgaa cctgcccgac cgctccgacg ccgagctcgt ggaggcggcg
    11581 ctggcccagc tccggcgcga cgatggggcc ttcgacgagt actgcgtagg gctgtcggac
    11641 gccgaccttg ccgcgctcga gtccgaggcg gtggagcggg tgctcctctt ccgggccacg
    11701 tttcccccgc tccagaaggc gtggtcgcct gcggtggagc cctcgctcaa ggcgacgttc
    11761 gcgggggacc gggtggtgct gtcggcccgg cccgatctgc tgctggggag cgacgatcgc
    11821 gaggagccga tgcgcgcgcg gcgcctggtg atcgagctga agacgggcgg cgaccggcct
    11881 gagcacgacg acgacctgcg gttctacgcg ctgctggcga cgctgcggct gggcgtgccg
    11941 ccgttccgcg tggccacggt gttcctcgac gacgggacgt ggcgcgcgca tgacgtctcc
    12001 gcggatctgc tcgaggccgc ggtgcgccgg atcggcgacg gcgcgatccg cgcgggggcg
    12061 atcatcgcgg gccacgagga gccgcggctc cgcccgggcc cctggtgccg ctggtgcccc
    12121 cgggccgaaa cctgccccga gtcgatgttc cgcgccgatg gctgacgttc tgcataggct
    12181 agcgtctcag gctgtctgat ggacacagct ctcattcggg agagatctct caaggtagcg
    12241 tcgaggctcg gatatgacgt gctgcgagct cccgctcatc gaacacgatc tcagggctcg
    12301 cgaggcggat gaggtcctgg atcgcacgtt ggtgcttcat gtgatcactg ctgtctcgta
    12361 tgggttcccg cgcgaggccg cggagagttg gctgctggcc gagcacctcc ttgaggctgt
    12421 gtcagcgcgt gagcgtcggt acgtctcggc tcctgctcca aaggaagatg tggcgtatca
    12481 gcttgggacg aggtatctct ggacacctag gaaccttgga gagtccgctg tcaggcgagg
    12541 cgggcggcgc cgaaggcgag cgtggcgacg gcgaggacca ttgcggcgtt cgacacatag
    12601 aacgtccgga cgaacgtcct ggtgatacgc gtctcgaggt tggccagttt ggtgtccatc
    12661 ctcgcctcga gaagctgcag cttcgtatcc gttcgctcct cgagcccgcg gagaccctcc
    12721 aggagctctt cacgcagttc ctcgcgcgtg actgcgtggg acccgagctc gctcatgagc
    12781 gtcttcgcct cctgggcgcc gaggacctcc tcgagtctcc gatgcagacg cgctcgctca
    12841 tccaccgcca ttgttgtcct ccttcccgca gccgacaacc cggggggagg agaggcgagc
    12901 ctagcaccga gtactgacaa ggtctgcccg agtgagaacg ctcatggcgg tcgagcgacg
    12961 ccgaggtggc ccggccggcg acgaacgcaa tcatggcaac cgcaagcgct agcgtggagt
    13021 tgccgaccca gatcgcgcga acgatcgtgc aggtatgagc gtccagcgcg tcgatcatgc
    13081 gggcctcgaa ggcggcaagc ctcgcatcca tccttgcctc gaatctggcg aggctcgcgt
    13141 cgaggcctga gccgagatcc tccctcggtc tcaaccgtac cagtcaccga gctcgggtgg
    13201 atgaagcaac ctacgcccgg gtaacatccc aagcacgttt ccctcctcgg aaggagcgag
    13261 cgtgaccaag gacatctacg agatcggtga gatccccccg ctgggcgagg ccccgaagcg
    13321 gatgtacgcg caggtgatca ggcccgagcg gtacggcgag cctcaggacg ccttccagat
    13381 agaggagatc cccgtgcccg aagtcgggcc cgacgacgcc ctcgtcctcg tgatggcggc
    13441 cggcatcaac tacaacaacg tctgggccgc ccaggggatc cccatcgacg tcacgaaggt
    13501 gcacggcaag ctcggcgagc ccgacgactt ccacatcggt ggctccgacg cctcgggcat
    13561 cgtctacgcc gtcggagaga acgtcacgaa cgtctccgtg ggcgaccacg tcgtcgtcca
    13621 ctgcggccag tgggaccgcg aggccgacga cctccccgac ccgatgctcc atccctcctt
    13681 ccggatctgg ggctacgagt cgaactgggg ttccttcgcc cagttcacca agatccaggc
    13741 gcaccagctg ctgccgaagg cgccccagct cacctgggag gccgccgccg cccccaccct
    13801 ggtcggggcc accgcctacc gcatgctctt cggctggccc ccgcacaccg tcgaggaggg
    13861 cgacgtggtc ctgatctggg gcgcggccgg cggactcggc tccatggcga tccagctcgt
    13921 ggcgcaggcc gggggcaagc cgatcggcgt cgtctccagc gaggacaagt tcgagttctg
    13981 ccgcaagctc ggggccgtcg gcgtcatcaa ccggaaggac ttcgaccact gggggatgcc
    14041 gccccactgg accgacggtg aggcctatgg gaaatgggcc gagggtgctc gggcgttcgg
    14101 taaggcgatg tgggatgccc tgggggagag gcgaagtccg aagatcgtgt tcgaacaccc
    14161 cggtgagtcc accatcccca cctcgatctt cgtggccgac accggtggca tggtcgtgat
    14221 ctgcgccggg acgaccggct acaacgccgt ggtggatctc cgctacctgt ggacccggca
    14281 gaagcggttc cagggctccc acttcgccaa cgacgagcag tcgcgcgcct tcaaccagat
    14341 ggtgatcgac ggcacggtgg acccggcgct ctcgcggacc ttctcgttcg acgacatcgg
    14401 gaccgcccat cagctgatgt ccgagaacgc ccaccccgac ggcaacatgg ccgcgctcgt
    14461 ctccgccccg agcgaggggc tcacggacgt gcccgcgtga ggcgggccct cgtcctgacg
    14521 ttcgccctcg tcgccgcggc ctgcggcggc gaggcggagg gcgaaggcga cgtcgccacc
    14581 gacgtctggg tccgcgacgt ctgcacgacg ctcacggact ggaacgacag ggtgcagagc
    14641 aggacaggca ccctccagac ggatctccag gggctccccc agggcgactt ccaggccctg
    14701 aaggacctca tggtctcgta catcgacggc gtgatcgagg acaccgatca agccgtgacc
    14761 tcgctcgaag gggcgggcgc cccagcggtg gaggacggcg gggaggcctc ccgcctggtg
    14821 gtccgcggga tcggggaggt ccgcacgatc ttcgagcagg cccgggagga catcgcggcg
    14881 ctcgacgcgt cgcgccctcg ccgcttcgcg atggccctcc aggacatcgg gctgcgcgtc
    14941 cagcagggcg ggcagcaggt ccaggccacg ttccaggagg tcgaccagcg cgggatcggg
    15001 ggggaagagc tcgaccaagc cttcgacgag gagcccgcgt gcgcgcctct cgccggaggg
    15061 gccggcccct aggcctcctg caccagctcg attagcgtgc cgtggagggc cttcgggtgg
    15121 acgaaggcca ccatcgtgcc gcgtgatccc gggcgtccgc gctcgtccac gagccgatgc
    15181 ccctgctcca tgaggtgggc gaggacctct tccacgtcgt tcacgcggta ggcgatgtgg
    15241 tggatcccct cgccgcgcgt cgcgaggtac ctcccgaccg gggtgtccgg gccgcgggac
    15301 ccgagcagct ggatgtacga ggtacccacc ttgaacatca cctcgtccac cccctggtcc
    15361 tcgacggtct cgcggtgcgt gggctccacg ccgaaggcgg cgcggtaccg ctccaccgcc
    15421 tcatcgaggt cctggacggc gatccccacg tgatcgatgt cggtcagcac tacctcgtcc
    15481 tccgtcgccc caggtccacc gccgggtgga ggcgcgcggc cagctcctgc gcctgcggga
    15541 catggtacgc gggggaggag agcgcggcgt ggatcgcggc gaccgcgcgg agcctggctt
    15601 cctcggagac cccgtcggcc ccaacgtaga agtcgatcag ctggaggaac tggggcaggc
    15661 tgggcaggag cccgccccgc tcgtacgcgt agatcgtctg cagggcggcc ggacacccct
    15721 tcgcctgcgc ggcctcgcgc acggcccggt agccgcaggt cttccgtacc ccccggagca
    15781 ggcctccgat gtggcgggcg gcgtccgcgg ggaccggttc gcgcttcttc cgtgccatcc
    15841 gcggtccttc ccgggtaggg ctccagtggc acgattctgc ccgcccgggt cggcgatcgc
    15901 caaatcgagc ggggtggtgg aatcctcgga ttccaggtac aactactcgt gggacccggg
    15961 ccgagcgttg cgcgcgcggc ccgggcatcg tcgtctctgg atgggagggc acgtgggcga
    16021 ggccgatcgc ggcggcaggg cgcaggagca ggcggcgacc aacgacgtgg cggcggtcct
    16081 cggacagtcg accctgatca aggcgggacg ggccgacgtc tcgaggatcc gggagcggac
    16141 gcgacgccgt cgactggtcc gcctcgcgct ggtgatcggc ctgatcgacg gctacctctg
    16201 gtaccggtac gcgtcgggga acccgatcgg ccttccctcg ctgccccagg acgcgtggat
    16261 ctggctgccg gggctcctgc tcgtcgccct cttcgccatc gtgatcctcc tcccgatggg
    16321 gagcggacgc tcgccggcca tggtcgtgcg gcccgagcac atcgaggtgg ggctcgagga
    16381 gctgaagggc ctcgacgccc aggtcgacga cgtgatccgc acgctcaacg tgttcctcgg
    16441 gtactcgacg ttccgctcgc ggctcggcgg aaacccccgg cgcggcgtgc tgttcgaggg
    16501 cccgcccggc accgggaaga ccttcctggc gaaggcgatg gcgaagcagg cgggcgtgcc
    16561 gttcctgttc gtctc
//
LOCUS       DASUTZ010000002         8021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1684593, whole genome shotgun sequence.
ACCESSION   DASUTZ010000002 DASUTZ010000000
VERSION     DASUTZ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8021
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1684593"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..552)
                     /locus_tag="VF097_00115"
     CDS             complement(121..552)
                     /locus_tag="VF097_00115"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX6261234.1"
                     /translation="MSDAASVLVLVARIVIGFYFSIFAGILGHIMASKGMEGYASAMR
                     FPVPGIAGWPTGIWLMLGGISVALGIYGDIGSLMIIAFLVLAAAWFHRFWQVGEDMKL
                     MQTGFFWRNVLTSAGLLVLFALFAELGPEMPYTITDSLISF"
     gene            848..2761
                     /locus_tag="VF097_00120"
     CDS             848..2761
                     /locus_tag="VF097_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX6261235.1"
                     /translation="MRRKVKLAWLVGAVVSVALLALPPAPAGADHNNDIHSPNISYIG
                     TAVNTDPMNPTNSDLAFWGNRAYVADYNGFRIIDITNPLDIADPSNVIADVVCPGPQF
                     DISVWDNDGDPAADLLFASVDTPKAGPDCGEANTTASNVESWEGVRIFDINDEENPVQ
                     IAAVPTDCGSHTHTIAGETTIADVDYLVIWVSSYPLGGGAVTTSIPARADVEVDSVEF
                     DFPAGVRDNGTECLEPEPGEAKPNGVHDKISIIRVPLNDPAAADDRTPNDPDPGEATN
                     WVYTNVREQTLPLAGIVTHINIGGRYFDVTACHDINTFLEINRAAGACWAEGIIWDIT
                     NPWQPTYLRRVRQPDVDTLFHSATFSYDGKIIVFEDEAGGGGENRCKLEDGEPDRQGA
                     MYFYKMNGVFQGRFKIPRQATGICTAHNYNTVPTTDGRRIAVSAWYTGGTSVIDYTDP
                     TNPKEIGFYRANSPVNGVGPDVGANTWSSYWYNGFIWANDIVRSLDLFSFDDPRVESA
                     IDLPHLNPQVQENVIQPVIPRCMGQEATIFGTSSGDVLTGTAGRDVIAGLGGNDLIRG
                     RGGNDLICGGAGADTIRGNAGDDRIQGQGSRDTLFGNKGNDVLNGGPARDSCLGGPGR
                     DRARNCERTRGIP"
     gene            2891..3922
                     /locus_tag="VF097_00125"
     CDS             2891..3922
                     /locus_tag="VF097_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEX6261236.1"
                     /translation="MARLRRADTGQPGIERRRRGRGFEYRGPDGRKVDDPEVLARIRS
                     LAIPPAWKDVWICLDPRGHLQAIGTDAAGRRQYLYHEAWRRRRDRQKFDRILDFAERL
                     PSLRRRLRRDLRLEGMPRERALACAVRLLDLGFFRIGSEEYAEENGSYGLATLLKDHV
                     MVGDSRAAFDYVAKGGARRVLEVSDPLVLPVLRTLRRRRGGGPELLAYREGRAWRDLR
                     STDVNEYLKGHVREQHSAKDFRTWTGTLLAAVALASHDELGRSRAARTRQVSQAVKSV
                     ASYLGNTPSVCRASYIDPRVIDLFLAGQTIAHTLRGGATTDLERLHGAAERATIRMLR
                     DAEPAARAA"
     gene            complement(3961..6324)
                     /gene="secD"
                     /locus_tag="VF097_00130"
     CDS             complement(3961..6324)
                     /gene="secD"
                     /locus_tag="VF097_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX6261237.1"
                     /translation="MSTNLGWRTFLVLVVLGGAIALILTRPVRLGLDLRGGTQIVLEA
                     RDTENVEVGGDVVDRTVEVLRRRVDALGVTEPTLQASGSRRIIIELPGVSDPDEAIRV
                     LGRTAQLTFHRVLGVEGPQAGPSPGPSPGMDEGDEEGEIVLPDESGGELRLARAALTG
                     EVVSDAQATIQQEFGGWTVQLRFRGEGSDAWAELTGRAACEPLGDPRRRIAIVLDDQI
                     ISSPEVDQSVVCEEGITGGETVITGNFTEDEARNLAVLIRGGALPVPVEIVEQRTVGA
                     TLGDEAIRASIQAALIGAALTILYMLVYYRLLGALAALALGAYAIIAFGALLALGATL
                     TLPGIAGFVLAVGMAVDANVLVFERIKEEHADGRSVRASGSAGFERAWSAIADSNATT
                     LLAAILLFFFASGAVRGFGITLSIGVVASMFSALVVTRVLVELAVRARRVRERPGLLG
                     MGVGHRFREWLEETRPDIVSHSRRWFTVSLIALVLAGVGLAVRGLRFGVEFQGGRLVE
                     YSTGQQVALDEVRAELADVGFPRAIVQRTGEGNVSIRTGRVTDAEEARIEQAVASLGG
                     TVSVEREEFVGPTISGELLRAAVIALVIALALQLVYLAMRFRWTFGTSAVVAMFHDVA
                     ILLGIFAWLGKEIDGVFLASLLTVIGYSINDSVVVFDRIRERWSERSREPFGRVANDA
                     ALQTVPRTINTGLGALFILVGLYLLGGDTLTDFALALIVGILVGTYSSIFVASPLVVT
                     LESGGGGPPVPARPAASTVAPSAKKAAPRPRRPARPKARSRSRKKGR"
     gene            complement(6372..6737)
                     /locus_tag="VF097_00135"
     CDS             complement(6372..6737)
                     /locus_tag="VF097_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261238.1"
                     /translation="MARRVLVRGLAAAFVAMLVPGTAWWEPAADRARAPARPRSESMF
                     APNPSPLGLLGGRIEESWATRRDERTATGDQVLGALLIGVAIVLLAGTERVAARRTRR
                     STPWTVRALGARSPPLAPA"
     gene            7030..>8021
                     /locus_tag="VF097_00140"
     CDS             7030..>8021
                     /locus_tag="VF097_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261239.1"
                     /translation="MRLATKLLLALLLPLMMIVASPVSASHSADDHSDNMREIALSPR
                     SSPATQSDLAFVGKMAIAGNYNGFRIFDISDPENPTLVKDVFCRGQQGDVSIWGDALV
                     VSVDEVLTGPECGSGPAVPATLETGWEGLRVFSLSEVLSTPADPDGFTRLQPAADIYI
                     KCGSHTHTGIPHKGHVDIYVASYPLRSGPDCGPHDDPSDTHNPLHEILSIARVDPQHP
                     EQSGGVIEVPIDIPTFNEDPLFLPPPAFNPMRGCHDIQVHLQLKLAAAACSSVGQVWD
                     ISDPHNPGTLDPVWEVDEPEVQFYHSALFSDDKTTVIFGDEIIVGHCFDGTGSG"
BASE COUNT         1334 a         2922 c         2663 g         1102 t
ORIGIN      
        1 ggctcgtcca ggtcgtgggg gtcctcggct gcgcgatcct cgccttcacg ctgccgccgg
       61 tctcggtggt gaccggcgcc gcggttctgg cggccggggc cgtcgcctgg gtcgccttcc
      121 tcagaacgag atcagcgagt cggtgatcgt gtatggcatc tccggcccta gctccgcgaa
      181 gagggcgaag aggaccagga ggcccgcgct ggtgaggacg ttcctccaga agaagcccgt
      241 ctgcatcagc ttcatatcct caccgacctg ccagaagcgg tggaaccacg cagccgcgag
      301 gacgaggaag gcgatgatca tgagggaccc gatgtcccca tagatcccca aggccaccga
      361 gatgccgccc agcatcagcc agatccccgt cggccacccc gcgatcccgg gaacggggaa
      421 gcgcatcgcg ctcgcgtacc cctccatgcc cttgctggcc atgatgtggc cgaggatccc
      481 ggcgaagatg ctgaagtaga agccgatcac gatccgcgct accagcacga gcacactcgc
      541 agcgtcgctc accgtcacct cccttggtcg ctgggcgcat agaagcctgc ccgaacccca
      601 ctgtgttcca cgtcccgact ccttcctctg ccgcccggag ggagtcgcaa gatggcttgc
      661 tccgctctgt cacgatcagg aggcgatcca ccgttccgtg agtttgcacc ctgaaagtcc
      721 ttcgggctat acatcgctgg gcaggagggg cagtccgggg aggtggtgcc tcactctcgg
      781 agcgatcgag tcgggtgaag gtagtcggac ccgacgaagg gtccatccgt cgcttgagga
      841 gggatcgatg cgcagaaagg tgaagctcgc ctggctggta ggcgccgtgg tctcggtggc
      901 gctcctggcg ctgccgccgg cacccgccgg cgcggaccac aacaacgaca tccacagtcc
      961 caacatcagc tacatcggca ctgcggtgaa cacggacccg atgaatccca cgaactccga
     1021 cctggcgttc tggggcaacc gcgcgtacgt ggccgactac aacgggttca ggatcatcga
     1081 catcacgaac ccgttggaca tcgccgatcc gagcaacgtg atcgccgacg tcgtctgtcc
     1141 aggaccgcag ttcgacatct cggtctggga caacgacggc gatccggcgg cggacctgct
     1201 cttcgcgtcg gtggacacgc cgaaggccgg gcccgactgc ggggaggcca acaccacggc
     1261 gagcaacgtc gagtcgtggg aaggggttcg gatcttcgac atcaacgacg aggagaaccc
     1321 cgtccagatc gccgcggtgc cgaccgactg cgggtctcac acccacacga tcgcgggtga
     1381 gaccacgatc gcggacgtcg actacctggt catctgggtg tcctcgtacc cgctgggagg
     1441 cggcgcggtg accacgagca tcccggctcg cgccgacgtc gaggtcgaca gcgtggagtt
     1501 cgacttcccg gcgggagtga gggacaacgg tacggagtgc ctggaacccg agccggggga
     1561 ggccaagccc aacggcgtcc acgacaagat ctcgatcatc cgcgtgcctc tgaacgatcc
     1621 agcggcggcg gacgaccgaa cgccgaatga cccggacccg ggcgaggcca cgaactgggt
     1681 ctacacgaac gtgcgggagc agaccctgcc gctggccgga atcgtcacgc acatcaacat
     1741 cggcggcagg tacttcgacg tgaccgcctg ccacgacatc aacacgttcc tggagatcaa
     1801 cagggcggcc ggcgcctgct gggccgaagg catcatctgg gacatcacga acccgtggca
     1861 gcccacctac ctgcgccgcg tccgccagcc cgacgtggac accctgtttc actcggccac
     1921 gttctcctac gacggcaaga tcatcgtctt cgaggacgag gcgggcggcg gcggcgagaa
     1981 ccgctgcaag ctcgaggacg gggagcccga ccgccagggc gcgatgtact tctacaagat
     2041 gaacggcgtg ttccagggcc gcttcaagat cccgcggcag gcgaccggga tctgcaccgc
     2101 gcacaactac aacacggtgc cgacgaccga tgggcggcgg atcgccgtct cggcctggta
     2161 cacgggcggg acctcggtga tcgactacac ggacccgacg aacccgaagg agatcggctt
     2221 ctacagggcc aactccccgg tgaacggcgt cggtccggac gtcggggcga acacgtggtc
     2281 ctcgtactgg tacaacggct tcatctgggc caacgacatc gtccggtccc tggacctgtt
     2341 ctcgttcgac gacccgcgcg tggagtcggc catcgacctc ccgcacctga acccgcaggt
     2401 gcaggagaac gtgatccagc cggtgatccc gcgctgcatg ggccaggagg cgacgatctt
     2461 cggcacgagc tccggtgacg tgctgaccgg aaccgcgggt cgagacgtca tcgccggcct
     2521 cggcggcaac gacctgatcc ggggtcgggg cggcaacgac ctgatctgcg gaggcgccgg
     2581 cgcggacacg atccgcggca acgccggtga cgaccgcatc cagggtcagg gaagcaggga
     2641 cacgctgttc ggcaacaagg gcaacgacgt gctgaacggc ggcccggcca gggacagctg
     2701 cctcggcggc ccggggcggg accgcgctcg caactgcgag cggacgcgcg gcatcccgta
     2761 gggtcctcga gaacagcgag gaagggcggc gccgcccccg aacgggggcg gcaccgccct
     2821 tctcccggtt tgccgcactc ccccaagcgg gaatcacttc ccgatatcgg cgagcacggg
     2881 aggtgacgag gtggctcggc tgcgtcgggc ggacaccggc cagcccggca tcgagcggcg
     2941 acggagaggt cgtggcttcg agtaccgggg gccggacggc cgcaaggtcg acgatcccga
     3001 ggtcctcgcg cggatcaggt cgctcgccat cccaccggcc tggaaggacg tctggatctg
     3061 cctcgaccca cgcgggcatc tccaggccat cggcaccgac gcggccggcc ggcgccagta
     3121 cctgtaccac gaggcctgga ggcggcggcg ggaccgccag aagttcgacc ggatccttga
     3181 cttcgcggag cggctcccct ccctccgcag gcgcctccgc cgggacctcc ggctcgaggg
     3241 gatgccacgg gagcgggccc tcgcctgcgc cgttcgactt ctcgacctcg gcttcttccg
     3301 catcggctcc gaggagtacg cggaggagaa cggcagctac ggcctggcca ccctcctcaa
     3361 ggaccacgtg atggtgggcg actcgcgggc ggcattcgac tacgtggcga agggcggcgc
     3421 ccggcgcgtg ctcgaggtct cggatccgct cgtgctgccc gttctccgga ccctgaggcg
     3481 gcgcaggggc ggcggccccg agctgctggc ctaccgagag gggagggcct ggcgggacct
     3541 gcgctccacg gacgtcaacg agtacctcaa agggcacgtc cgcgagcagc actccgccaa
     3601 ggacttccgg acgtggaccg ggaccctact cgccgccgtc gcgctcgcct cccacgacga
     3661 gctgggccgg tccagggcgg cccggacgcg tcaggtgagc caggccgtga agtccgtcgc
     3721 ctcctacctg gggaacacgc cctcggtgtg ccgggcgtct tacatcgatc cccgcgtcat
     3781 cgacctgttc ctcgccggtc agacgatcgc ccacaccctg agaggtgggg cgaccacaga
     3841 cctggagcgt ctgcacggcg ccgccgagcg ggccacgatc cgcatgcttc gcgacgcaga
     3901 gcctgccgcc cgcgcggcct gagggcctga atcgagccgc tcagcagggc ggcctatgcc
     3961 tcatcgcccc ttcttccgtg agcgcgacct cgccttgggc cgcgcgggcc gccggggccg
     4021 aggggccgcc ttcttcgcgg atggcgcaac ggtgctcgcg gccggacggg ccgggaccgg
     4081 cggcccgccc ccgccgctct ccagcgtgac cacaagcggc gaggccacga agatcgacga
     4141 gtacgtcccc accaggatcc ccacgatcag cgccagcgcg aagtcggtca gggtgtcccc
     4201 gcccaacagg tacaggccca cgaggatgaa cagggctccg aggcccgtgt tgatcgtccg
     4261 gggcaccgtc tgcagggcgg catcgttggc caccctcccg aacggctctc ggctgcgctc
     4321 gctccatcgc tcacggatcc ggtcgaagac cacgaccgag tcgttgatcg agtacccgat
     4381 cacggtcagg agcgacgcga ggaagacacc atcgatctcc ttccccagcc aggcaaagat
     4441 gcctagcagg atcgcgacgt cgtggaacat cgccaccacc gccgacgtcc cgaacgtcca
     4501 ccggaagcgc atggcgaggt agaccagctg gagggcgagg gcgatgacga gcgcgatgac
     4561 cgccgcgcgg agcagctccc cgctgatggt cgggcccacg aactcctccc gctccaccga
     4621 gaccgtcccg cccagcgacg cgacggcctg ctcgatccgg gcctcttccg catcggtcac
     4681 ccggcccgtc cggatcgaga cgttgccctc ccccgtccgc tgcacgatgg cccgcgggaa
     4741 cccgacgtcg gcgagctccg cgcgaacctc gtccagcgcg acctgctgac ctgtcgagta
     4801 ctccacgaga cgcccaccct ggaactccac cccgaaccga agcccgcgca ccgcgagccc
     4861 caccccggcc agcaccaggg cgatcagcga gaccgtgaac caccggcggg agtggctcac
     4921 gatgtccggg cgcgtctcct ccagccattc gcggaaccgg tggccgaccc ccatccccag
     4981 gagcccgggc cgctcgcgca cacgccgcgc cctcacggcg agctcgacca gcacacgggt
     5041 cacgacgagg gcgctgaaca tcgaggccac gaccccgatc gagagggtga tgccgaaccc
     5101 gcgcaccgct cccgaggcga agaagaacag caggatcgcc gccagcagcg tcgtggcgtt
     5161 cgagtcggcg atcgcgctcc aggcccgctc gaacccggcg ctccccgagg cgcggaccga
     5221 tcggccgtcg gcatgctcct ccttgatccg ctcgaagacc agaacgttcg cgtccaccgc
     5281 catcccgacg gcgagcacga acccggcgat ccccggcagg gtgagggtgg cccccagcgc
     5341 cagcagcgcg ccgaacgcga tgatggcgta cgcgccgagc gcgagggcgg ccagcgctcc
     5401 gagcagccgg tagtagacga gcatgtacag gatcgtcagc gcggcgccga tcagcgccgc
     5461 ctggatgctg gcgcggatcg cctcgtcccc cagggtcgcc ccgacagtcc gctgctcgac
     5521 gatctcaacc ggaaccggaa gggcgccgcc ccggatcagc accgcgaggt tcctcgcctc
     5581 gtcctccgtg aagttccccg tgatcaccgt ctcgccgccc gtgatccctt cctcgcagac
     5641 gacgctctgg tcgacctcgg ggctggagat gatctgatcg tcgaggacga tcgcgatgcg
     5701 ccgccgcggg tcgcccagcg gctcgcacgc ggcgcgcccg gtcagctccg cccaggcgtc
     5761 ggacccctcg cccctgaacc gaagctgcac cgtccacccg ccgaactcct gctggatcgt
     5821 ggcctgcgcg tcgctgacga cctcgcccgt cagggccgcc cgcgcgagcc ggagctcccc
     5881 tcccgactcg tccggcagga cgatctcgcc ctcctcatcc ccctcgtcca tgcccggcga
     5941 cgggccgggc gagggccccg cctgcgggcc ctccacgccg agcactcgat ggaacgtcag
     6001 ctgcgccgtg cgccccagga cgcggatggc ctcgtccggg tcggacacgc cggggagctc
     6061 gatgatgatg cgccggctcc cggaggcctg gagggtgggc tcggtcacac ccagggcgtc
     6121 cacgcgcctt cgcaggacct ccacggtgcg atcgaccacg tcgccgccca cctcgacgtt
     6181 ctccgtgtcc ctcgcctcga ggacgatctg ggtcccgccc cggagatcga gcccgagccg
     6241 aaccggcctc gtcaggatga gggcgatggc ccccccgagg acgacgagga cgaggaacgt
     6301 gcgccatccg agattcgttg acatcgagat cctccggccc ggcgccgccg tgcggcggcg
     6361 cgtttcacca gctaggcggg cgcgagcggg ggcgaccgtg cccccagcgc ccggacggtc
     6421 cacggggtcg agcgtcgcgt tcggcgtgcc gccacgcgct cggtcccggc gagcaggacg
     6481 atcgccacac ctatcagcag cgcgccgaga acctggtcgc ccgtcgctgt tcgctcgtcg
     6541 cggcgcgtgg cccagctttc ttcgatcctg ccgcccagca ggcccagcgg cgaaggattg
     6601 ggcgcgaaca tcgactccga ccggggacgc gccggggcac gagcgcgatc cgccgcgggc
     6661 tcccaccacg ccgtgccggg cacgagcatg gcgacgaacg cggctgccaa tccccggacg
     6721 aggacccggc gcgccatccc cgctacccta ccggacggtc ggggggcccg tggcggagtc
     6781 tcggagtcgt ctcgtcctgg ggaagggttc cgcccgacgg cggcatctgg gcccagggcg
     6841 cggtctcctg cccgcaggca cgcgggagcg tcccgcccac acagaccgtc cgtgatttga
     6901 ccctctccta cagggactag taggttgctc gggacaccgc ggaggcgcgt ccgaaggggg
     6961 tgatggatga ccgagccata gcggaagctc atccttaccc gccccacccg cccaggaggt
     7021 gacgatcccg tgagactcgc aacgaagctc ctgctcgcgc ttctcctccc tctgatgatg
     7081 atcgtggcgt cccccgtgag cgcctcgcac agcgcggacg atcacagcga caacatgagg
     7141 gagatcgcgc tcagcccgcg gtccagcccg gccacccaat ccgacctcgc cttcgtcggg
     7201 aagatggcga tcgcggggaa ctacaacggc ttccggatct tcgacatctc cgatccggag
     7261 aatcccacgc tcgtcaagga cgtcttctgt cgagggcagc agggcgacgt ctcgatctgg
     7321 ggcgatgcgc tcgtcgtctc ggtggacgag gtcctgacgg gccccgaatg tggatccggc
     7381 cccgcggtcc cggcgacgct ggagaccggc tgggagggcc tgcgcgtgtt cagcctgagc
     7441 gaggtcctgt ccaccccggc ggaccccgac ggcttcaccc ggctccagcc cgcggccgac
     7501 atctacatca agtgcgggtc gcacacccac actgggatcc cgcacaaggg gcacgtggac
     7561 atctacgtgg cgtcctatcc gctccggtcg ggccccgact gcggccctca cgacgacccg
     7621 tccgacacgc acaacccgct gcacgagatc ctctcgatcg cccgggtcga cccgcagcac
     7681 ccggagcagt caggcggtgt catcgaggtc ccaatcgaca tcccaacctt caacgaggac
     7741 cccctcttcc tgccgccgcc cgcgttcaac ccgatgcgcg ggtgccacga catccaggtg
     7801 cacctgcagc tgaagctcgc ggccgcggcg tgcagcagcg tcgggcaggt ctgggacatc
     7861 tcggatccgc acaaccccgg gaccctggat cccgtgtggg aggtggacga gccggaggtg
     7921 cagttctacc actcggccct gttcagcgac gacaagacca ccgtgatctt cggcgatgag
     7981 atcatcgtgg ggcactgctt cgatgggacc gggagcggcc a
//
LOCUS       DASUTZ010000003         8503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_648332, whole genome shotgun sequence.
ACCESSION   DASUTZ010000003 DASUTZ010000000
VERSION     DASUTZ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8503
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648332"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..152)
                     /locus_tag="VF097_00145"
     CDS             complement(<1..152)
                     /locus_tag="VF097_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HEX6261240.1"
                     /translation="MSDPSLSVPSEPIEHPVPIEDHGLIGDTRTAALVTRRGEIDWLC
                     LPRFESP"
     gene            complement(211..771)
                     /locus_tag="VF097_00150"
     CDS             complement(211..771)
                     /locus_tag="VF097_00150"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HEX6261241.1"
                     /translation="MRRSILVLGMLTAVAAVGCADEGDEPGFSPPPTPTGALTASPSP
                     SPTTETHVVRVFFGAPDAAETECAEVVPVERTTEGADVLHDAMEALLEGPTRDERAQG
                     IGSFFGAKTRGMLNAVRIEDGVARVDFRDFSQVIPNASTSCGSAALLAQLDATATQFP
                     TVREARYSFEGSEEAFYEWLQMSVPT"
     gene            complement(875..2323)
                     /locus_tag="VF097_00155"
     CDS             complement(875..2323)
                     /locus_tag="VF097_00155"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020556461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6261242.1"
                     /translation="MRNQMLIAGSWTDGRGSESIAVTSPVTGEGLGEVPVATPEDVDE
                     AVGAARRAVEALDRMTVFERAERLHRVAALMRERKEVLARELTLEHGKPYKAEALDEI
                     EESAENFEVAAEDIKRVNTEVLPSTDRNKRMFTLRKANGVYATITPWNFPFVIPSELL
                     APGLAAGNAMILKPSEHTPLIGARMAEIIAEAGFPEGSISLVFGAGETGRTLVSHPGV
                     DAIAFVGHNSTAEAIVRAAGLKRTLMEASGNGPVIVLDDADLDEAAAMATFGATYVSG
                     QCCVATERILVSESVHDDFLERLVKEAEAAVLGDPFDESTIMGPLNNADVAEKMDRHL
                     EDAREKGVAILTGGGRADGFPTDLYYELTVADGVGPDTLLFREETFGPVLPVTTFRDD
                     EEAIAMANDSDLGLQAAVFTSSLRRAFRFIDRIRAGNIVVNDTTDYWEAHQPFGGASG
                     TKTGWGRVGGKYAIEDMSDLRTVVIDWEKTKD"
     gene            2410..2483
                     /locus_tag="VF097_00160"
     tRNA            2410..2483
                     /locus_tag="VF097_00160"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2444..2446,aa:His,seq:gtg)
     gene            2528..2601
                     /locus_tag="VF097_00165"
     tRNA            2528..2601
                     /locus_tag="VF097_00165"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2562..2564,aa:Lys,seq:ctt)
     gene            complement(3250..3861)
                     /locus_tag="VF097_00170"
     CDS             complement(3250..3861)
                     /locus_tag="VF097_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261243.1"
                     /translation="MISTRAGWGASMVGLALAVSLFAGALLQPVLVGSADAHVTSKVS
                     HLWKRHIKKMADRRYLNQRTSTHTVPFAAFGPLTSSQPYAMGGQFVSGQSGTGALFAP
                     VTLPQRARITTVACHFYDVSPVSDVSCRFRWAPTGQPMVSSVAGNLVSSGNGGYQSVS
                     TAAIDQPVVNNKRRVYYVQIAPVSSWDGLNAALVGVEITYTLE"
     gene            complement(3939..4466)
                     /locus_tag="VF097_00175"
     CDS             complement(3939..4466)
                     /locus_tag="VF097_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX6261244.1"
                     /translation="MTGFSVREAREILARTPGVLDRLLSGASSGWTHEAPPGAWTPAE
                     ILTHLIHGEKTDWVPRVRIILEEGPSRPFEPFDREGGAVEAGRPVEELLAELSRLRVR
                     NLAYLDGLELDDEALARKGTHPELGTVTLEQLIATWAAHDLGHVGHVVEVMSKRYRET
                     VGPWRAYLPFLDRPD"
     gene            complement(4501..4797)
                     /locus_tag="VF097_00180"
     CDS             complement(4501..4797)
                     /locus_tag="VF097_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261245.1"
                     /translation="MGGRVLGLVFVAMVVVAGCGEQVETVGTTEECIRKVGSVVDQEF
                     LEERNSSLVEIGAGKGDLVGAIAKACVDSSPEDPVEEVAERAVKILNPDPPEEK"
     gene            complement(4836..5465)
                     /locus_tag="VF097_00185"
     CDS             complement(4836..5465)
                     /locus_tag="VF097_00185"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX6261246.1"
                     /translation="MVALLEEIVEFIGPLFSGFTGYAIVTAAVLLERSILIGVVVPGE
                     VTMAAGALFAARGQLSLTLVIVLGALAAMVGESIGFWLGRRYGRSLMRRLPLLNRFEH
                     RIEDVEELFDRHGGKAVAVGRFAAGAGVFIPFVAGMGSMRYKRFLLFDVPSIVIWALG
                     VGFVGYLLGRNIELLDRILTNFGWAMLGLLVVGIVAYVIWKRRRDSTDD"
     gene            complement(5466..6287)
                     /locus_tag="VF097_00190"
     CDS             complement(5466..6287)
                     /locus_tag="VF097_00190"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX6261247.1"
                     /translation="MAEHRLISKTIDWVQDRVPKPVGDFIGRLREEDVLLPASGLAFY
                     ALVSVAPLLIVSVWVVGLMVSDTRIQQLSQQVKDVAPPGLGLGNLIQRVASQGTTLGI
                     GAVVAALWPATAYGSGLAQGFERLHHSDGGEIPGLRGRGLAILVILPMFVLGILGAAF
                     LGTTLLKGGITASIFGPVLALVFGFAAAFVGTAVIYWIFPVHRLPWGTLARAAALAAA
                     LIAVLSLLFVLYLNLGADFEKSYASSTIAGMVLLGVWLFLSNAVLLAVHAGADER"
     gene            6356..6438
                     /locus_tag="VF097_00195"
     tRNA            6356..6438
                     /locus_tag="VF097_00195"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6389..6391,aa:Leu,seq:tag)
     gene            complement(6404..7288)
                     /locus_tag="VF097_00200"
     CDS             complement(6404..7288)
                     /locus_tag="VF097_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261248.1"
                     /translation="MREIAERLGATDLFVLYRVAPQRLVNVDGHGRGAGWVGNIDLDP
                     STERWLTVVQERGLARVSQGMPQRIFGPYWATQAVGVAVGDYVVVLGGWDVANLPDEG
                     VRDAAALAVAAVAEVPVAKRLADDLEVTQAALAVARMRPDSLDAAARAIATTAARATS
                     CEFGAVLLFGPPITLHLAEEGWRPSASEEEIAAALLPLAQALSDGLYVEQDTSESPFP
                     YRPLTFEDGLVARCAVRLGTEGDLGVLVVAHSLESPRGFTMLCQRVMRAIGETAQSTL
                     SSLIDSGVREGGFEPPRA"
     gene            complement(7281..8453)
                     /locus_tag="VF097_00205"
     CDS             complement(7281..8453)
                     /locus_tag="VF097_00205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6261249.1"
                     /translation="MSKRSLRLEEGVALALRAVRQPRTGAAAVLSAVARAMEAELVAA
                     YLLAGDPGRTAELIAAAGPRTPQLGPLGSVDEQTAAVLQGCLHEAVSEDSFFPEPWSS
                     FPRRAHAVDRLPLGGALVVLAAGEGLLDRDVLLRVMGPLDLLVTTMAVMGRQERLEAE
                     LLRIRQEQALLAASLQHDLRTPLTSVLGAAQTLLDRSASLSAEQRAELLEVIATQAER
                     LNGMIGETLARHAAGPDVPVRMSLVDPLAVARRVCGAARSARGGEIAIDVEPTPFLCD
                     ESRLERALLNLVDNALKYAPDGTEVHVIGAHTGGTYTFMVADAGPGVASEVAAAMFSP
                     YVTDPSRDDGTGLGLHSVAHVMEELGGKVSYARTSGWTRFSLALPWRGPDAGPPDA"
BASE COUNT         1352 a         3041 c         2889 g         1221 t
ORIGIN      
        1 gggctctcga agcgcggcag gcagagccag tcgatctccc cgcgacgcgt gacgagtgcg
       61 gcggtccgag tgtcaccgat gaggccgtga tcctcgatgg gaaccggatg ctcgatcggt
      121 tcgctcggga ccgagagcga agggtcgctc atgtctccat catctaccag caagcgggac
      181 tgttctggag acgacctcct ccccgatgcc tcacgtgggt acgctcatct ggagccactc
      241 gtagaaggcc tcctcgctcc cctcgaacga gtacctcgcc tcccgcacgg tcgggaactg
      301 ggtcgcggtc gcgtcgagct gggccaggag ggccgcgctg ccgcaggagg tggacgcgtt
      361 cgggatcacc tgggagaagt cccggaagtc cacgcgagcc acgccgtcct cgatgcgcac
      421 ggcgttgagc atcccgcgcg tcttcgcgcc gaagaagctt ccgatgccct gggcgcgctc
      481 gtcgcgggtc gggccttcga ggagggcctc catggcgtcg tggaggacgt ccgctccttc
      541 ggtggtccgt tcgaccggga cgacctcggc gcattcggtc tccgcggcgt ccggcgcccc
      601 gaagaacacc cgcacgacgt gagtctccgt ggtgggcgag gggctggggc tcgccgtgag
      661 ggcgccggtg ggggtggggg gcgggctgaa gcctggctcg tctccctcat ccgcgcatcc
      721 gacggccgcc accgcggtca gcatccccag cacgaggatc gacctgcgca tccacaaacc
      781 tccacgatga ctgtatccgg acggcgagcg ccggctccgg ggccgttcgg accgcttccc
      841 gcgggtccct ccgccgatag gcccgggcag cccctcagtc cttcgtcttc tcccagtcga
      901 tgacgaccgt gcggaggtcg gacatgtcct cgatcgcgta cttccccccg accctccccc
      961 agccggtctt cgtgccggaa gcgccgccga agggctggtg cgcttcccag tagtcggtcg
     1021 tgtcgttcac cacgatgttg ccggcacgga tccggtcgat gaaccggaag gcccgcctca
     1081 gactcgaggt gaagaccgcg gcctgcagcc ccagatccga atcgttggcc atggcgatgg
     1141 cctcctcgtc gtctcggaac gtggtcacgg ggaggacggg cccgaaggtc tcctctcgga
     1201 agaggagggt gtcgggcccg acgccgtcgg ccacggtgag ctcgtagtag aggtcggtgg
     1261 ggaacccgtc cgcccgcccc ccacccgtga ggatcgcgac gcccttctcg cgcgcgtctt
     1321 cgaggtgccg gtccatcttc tcagccacgt cggcgttgtt cagcggcccc atgatcgtcg
     1381 attcgtcgaa cgggtccccc agcacggcgg cctccgcctc cttcaccagc cgctccagga
     1441 agtcgtcgtg gacggactcg gagacgagga tccgctccgt agccacgcag cactgccccg
     1501 agacgtaggt cgcaccgaac gtggccatgg ccgccgcctc atcgaggtcg gcgtcgtcga
     1561 gcacgatcac cggtccgttg cccgaggctt ccatcagcgt gcgcttcagg cccgccgcgc
     1621 ggacgatggc ctccgcggtg ctgttgtggc ccacgaaggc gatcgcgtcc acgccggggt
     1681 gcgagacgag cgtccgtccc gtctcccccg cgccgaacac gagcgagatg gacccctccg
     1741 ggaagccggc ctcggcaatg atctcggcca tccgtgcgcc gatcagcggc gtgtgctcgg
     1801 agggcttgag gatcatcgcg ttcccggcag ccaggccggg agccagcagc tcggacggga
     1861 tcacgaaggg gaagttccac ggcgtgatcg tggcgtacac gccgttggcc ttccggaggg
     1921 tgaacatcct cttgttgcgg tctgtcgagg gaaggacctc ggtgttgacg cgcttgatgt
     1981 cctccgccgc gacctcgaag ttctcggccg actcctcgat ctcgtcgagg gcctcggcct
     2041 tgtagggctt cccgtgctca agcgtgagct cccgggcgag cacctccttc cgctcgcgca
     2101 tcagcgccgc cacgcgatgg aggcgttccg cgcgctcgaa gaccgtcatg cggtcgagcg
     2161 cctccacggc gcgtcgggcc gcccccaccg cctcgtccac atcctcgggg gtggcgaccg
     2221 ggacctcgcc cagcccctcc cccgttaccg ggctcgtcac cgcgatcgac tcggagcccc
     2281 gcccgtcggt ccacgagccc gcgatcagca tctggttgcg catggcatcc tcctcgggga
     2341 caggctaccc gcgcgggtcg ctcttcacga aggggcgagg gaggccactg ctacaatcga
     2401 tgcggcgtgg tggccgtagc tcaagtggtt agagcgtcgg gttgtggtcc cgaaggttgg
     2461 gggttcgagt cccctcggtc accctctcgt gggcgggcat gagggcgccc gctacactgt
     2521 cctcgtcggg ccgttagctc aaactggcag agcaggggac tcttaatccc aaggttgaag
     2581 gttcgagtcc ttcacggccc accgagatcg gcccatggac gaacggcccg cgaacaggac
     2641 ggaactcacc tcagcccagc agtggcaggc tggccccggg tactgccttc tcgcattgac
     2701 gagccaccgc ggccgtgggc tatgggctca ccccgggctc gcccgcgttc gttccggcct
     2761 gcggttgaac ggcattgacc tcgggtggta acggatccga gtacaagcgg taccgaacaa
     2821 gcttcccctc gctccagcga tgcgccaggg cgccctcgag gatcagcgag cggcccccga
     2881 tcgttgcctc aatcgtgatc tcggcgagga tctcgccgcc ggattcgagg tatcgtcgaa
     2941 tccagatcag tggcacagaa gcgcaggtgc caggaggctg ggagtcgagc gccaccttca
     3001 gagccgcatg gtgcgaatgc ctgacggcgc gagggatgcc tcttccatcc cggtcgacgc
     3061 cgccgaggac caccccggcc agtgggtgct gcgcagggtg gcctcgaacg acccggtctc
     3121 cgtggacaac cagatgttct cggtgggcaa cgcctgcaag gcgccagctc gtggacaaca
     3181 ccgtgatccg ggtctggagc aagaaccacc tcatcaagcg agtcaggccc accgacatcc
     3241 gcagcctccc tactcgaggg tgtaggtgat ttcgacgccg accagcgccg cgttcaggcc
     3301 gtcccaggag ctcacgggcg cgatctgcac gtagtacaca cggcgcttgt tgttgacgac
     3361 gggctgatcg atggcggcgg tcgagacgct ctgatagccg ccgttcccgg aggagacaag
     3421 gttgccggcc acggagctga ccatgggctg acccgtgggc gcccagcgga agcggcatga
     3481 gacgtcggac acgggggaga cgtcgtagaa gtggcaggcc acggtcgtga tccgggccct
     3541 ctgcgggagc gtgaccggag cgaagagggc cccggtgccg ctctggccgc tcacgaactg
     3601 gcctcccatc gcgtatggct gcgaggacgt gagcgggccg aaggccgcga acgggaccgt
     3661 gtgcgtggac gtccgctggt tcagatagcg gcggtccgcc atcttcttga tgtgccgctt
     3721 ccacaggtgc gagaccttcg acgtcacgtg ggcatcggcc gagcccacga gaacaggctg
     3781 aaggagtgct cccgcgaaga gcgacaccgc cagcgcgagc cccaccatcg aggctcccca
     3841 ccctgctcgg gtgctgatca tgacgacctc ctcggacgga cgagccgcga gcgtacgacg
     3901 aacccccttg ggatcggtga aacgccacct gcgaggtctc agtcggggcg gtcgaggaag
     3961 ggcaggtacg ctcgccaggg gccgaccgtc tcccggtacc gcttcgacat gacctcgacg
     4021 acatggccca cgtggccgag gtcgtgggcg gcccaggtcg cgatcagctg ctcgagggtg
     4081 accgtgccca gctcggggtg cgtccctttc ctggccagcg cctcatcgtc cagctcgagg
     4141 ccgtcgaggt acgcgaggtt ccgcaccctg agcctcgaga gctcggccag cagctcctcg
     4201 acaggacgcc ccgcctcgac cgccccgccc tcgcggtcga acggctcgaa cggcctcgac
     4261 ggaccctcct cgaggatgat ccggacgcga ggcacccagt cggtcttctc gccgtggatc
     4321 aggtgggtga gaatctcggc cggggtccag gctccgggcg gcgcctcgtg ggtccagccg
     4381 gacgaggccc cgctcagcag ccggtcaagc acccccggtg tgcgcgccag gatctcacgc
     4441 gcctcccgca ccgagaaccc ggtcacatcg cgagcatacc gggccgcgcg ttcgacgccg
     4501 ctacttctct tcgggggggt ccgggttgag gatcttcacg gctcgctccg cgacctcctc
     4561 cacgggatcc tcgggggagg aatccacgca cgccttggcg atggcgccca cgaggtcgcc
     4621 cttccccgcg ccgatctcga cgagggacga gttgcgctcc tccaggaact cctggtcgac
     4681 gaccgagccc accttccgta tgcactcctc ggtggtcccc acggtctcca cctgctcgcc
     4741 gcagccggcg acgacgacca tcgccacgaa caccaggccc aggactcttc cgcccacgct
     4801 cctccctcgg tcgaagcgtg agggtagcgg ctcgctcaat cgtctgtcga gtcgcggcgg
     4861 cgcttccaga tcacgtaggc cacgatcccc accacgagga ggcccagcat ggcccatccg
     4921 aagttggtga ggatccgatc gaggagctcg atgttccgcc ccagaaggta gccgacgaac
     4981 cccacgccca gcgcccagat gacgatcgac gggacgtcga agagcaggaa cctcttgtag
     5041 cgcatgctcc ccatccccgc gacgaacggg atgaagaccc cggccccggc cgcgaacctc
     5101 ccgaccgcca cggccttccc cccgtgccga tcgaacagct cctccacgtc ctcgatgcga
     5161 tgctcgaacc ggttcaggag cggaagtcgc ctcatcaacg agcggccgta ccggcgcccc
     5221 agccagaagc cgatcgactc gccgaccatc gcggccagag cgcccaggac gatgaccagg
     5281 gtcaggctca gctgccccct ggccgcgaac agcgctcccg cggccatcgt cacctctccc
     5341 gggaccacga cgccgatcag gatggagcgc tcgagcagca cggcggccgt cacgatcgcg
     5401 tagcccgtga acccgctgaa cagcgggccg atgaactcga cgatctcctc gaggagggcg
     5461 accattcagc gctcgtccgc gcccgcgtga acggcgagga ggaccgcgtt ggagaggaac
     5521 agccacacgc ccaggaggac catccccgcg atcgtactgc tggcgtagct cttctcgaag
     5581 tcggcaccaa ggttcagata gaggacgaag agcagcgaca gcacggcgat gagagccgcc
     5641 gccagcgcgg cggcgcgagc gagcgtaccc cagggcaggc ggtggacggg gaagatccag
     5701 tagatcacgg ccgtgccgac gaacgcggcg gcgaacccga agaccagggc cagcacgggc
     5761 ccgaagatcg acgccgtgat gccgcccttc aggagcgtcg tgcccaagaa tgccgccccc
     5821 aggatcccga ggacgaacat ggggaggatc accaggatgg ccagaccgcg cccccggagg
     5881 cccgggatct ctccgccgtc gctgtggtgg aggcgctcga agccttgagc cagccccgag
     5941 ccgtaggcgg tggccggcca cagggcggcc accaccgctc cgatccccag ggtcgtcccc
     6001 tgcgaggcca cccgctggat caggttgccg agccccaggc cggggggcgc cacgtccttc
     6061 acctgctggg acagctgctg gatccgcgta tcgctcacca tcaagccgac cacccacacc
     6121 gagacgatca ggaggggcgc caccgagacg agcgcgtaga aggcgaggcc cgacgcgggc
     6181 agaaggacgt cctcctcgcg gagcctgccg atgaagtccc caaccggctt cgggacccga
     6241 tcctggaccc agtcgatcgt cttggagata agcctgtgct cggccaccac cctcggttac
     6301 ccgggggccc gagcggggaa acggagcggg cgtggcggac cctatactcg ggtgcgcgag
     6361 agtggcggaa cggcagacgc gccggcctta ggagccggtg ggctcaggcc cgtgggggtt
     6421 cgaatccccc ctctcgcacc ccgctgtcga tgagcgacga gagggtcgac tgggccgtct
     6481 cgccgatcgc acgcatcacc cgctggcaga gcatcgtgaa gcctcgcggc gactccagcg
     6541 aatgggccac gaccaggaca ccgaggtcgc cctccgtgcc gagcctaacg gcacaccgag
     6601 ccacgagccc gtcctcgaac gtgagcggac ggtacgggaa cgggctctcg gacgtgtcct
     6661 gctcgacgta gagcccgtcg gagagggcct gggccagcgg gaggagggcg gccgcgatct
     6721 cctcctcgct ggcggagggc cgccagccct cctccgccag atggagcgtg atgggaggcc
     6781 cgaagaggag cacggcgccg aactcgcaag aggtggcccg ggccgcggtc gtggcgatcg
     6841 cccttgcagc ggcgtccagg gagtccggac gcatccgggc caccgccagc gctgcctggg
     6901 tgacctcgag gtcgtcggcc aggcgcttcg ccacggggac ctcggccacc gccgcgacgg
     6961 cgagggccgc tgcgtcgcgg accccttcgt ccggcaggtt cgcgacgtcc cacccgccga
     7021 gaaccaccac gtaatccccc accgcaacac ccacggcctg agtcgcccag tagggtccga
     7081 agatccgctg cggcatcccc tgggagacgc gggccaggcc ccgttcctgc accaccgtca
     7141 gccaccgctc ggtcgagggg tcgagatcga tgttcccgac ccatccggca ccgcggccgt
     7201 gaccgtccac gttcacgagc ctctgggggg ccacgcggta gagcacgaag aggtccgtcg
     7261 ccccgagacg ctcggcgatc tcacgcatcc ggcggtcccg catccggccc gcgccacggg
     7321 agggcgagcg agaagcgcgt ccacccgctg gtgcgggcgt aggagacctt ccccccgagc
     7381 tcctccatca cgtgggccac cgagtggagc cccaggcctg tcccgtcgtc ccgggaggga
     7441 tcggtaacgt agggagagaa catcgctgcg gccacctcgg aggcgacgcc gggcccggcg
     7501 tcggccacca tgaacgtgta cgtgccgccc gtgtgagcgc cgatcacgtg cacctcggtc
     7561 ccgtccgggg cgtacttgag ggcgttgtcc acgaggttga ggagcgctcg ctccagcctc
     7621 gactcgtcgc agaggaacgg ggtcggctcg acgtcgatcg cgatctcgcc cccccgggca
     7681 gagcgagcgg cgccgcacac tcggcgagcc acggccagcg gatccaccag gctcatgcgc
     7741 accggcacgt cgggcccggc ggcgtgcctg gccagcgtct ccccgatcat cccgttcagc
     7801 cgctcggcct gggtcgcgat cacctcgagc agctccgcgc gctgctccgc cgagaggctg
     7861 gccgaccgat cgagcagggt ctgcgcggcg cccaggacgg aggtcaacgg cgtgcggagg
     7921 tcgtgctgga ggctggctgc cagcagggcc tgttcctggc ggatccgcag cagctccgct
     7981 tcgagccgct cctggcggcc catcacggcc atcgtcgtga cgaggaggtc cagggggccc
     8041 atcacgcgca ggagcacgtc gcggtcgagg aggccttcgc cggccgccag caccacgagg
     8101 gcgcccccca gcgggaggcg atccacggcg tgcgcccggc gggggaagct gctccagggc
     8161 tccgggaaga agctgtcctc agagacggcc tcgtgcaggc acccctggag gacggcggcg
     8221 gtctgctcgt cgacggagcc gaggggaccg agctggggcg tccgaggccc ggccgccgcg
     8281 atcagctcgg ccgtcctgcc ggggtctccc gcaagcaggt aggcggcgac cagctccgcc
     8341 tccatcgccc gagcgacggc cgacagcacg gcggccgcgc cggtccgcgg ctgtcggacg
     8401 gcccggagag cgagggcgac accctcctcg agccggagtg atcgcttgct cacgcggaac
     8461 gcctccctga gcccaccccg agtgttatcg gcacggctca cgc
//
LOCUS       DASUTZ010000004         2418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_648508, whole genome shotgun sequence.
ACCESSION   DASUTZ010000004 DASUTZ010000000
VERSION     DASUTZ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648508"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1921)
                     /locus_tag="VF097_00210"
     CDS             complement(<1..1921)
                     /locus_tag="VF097_00210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6261250.1"
                     /translation="MTEYPAHREADVVLRDGSTVLIRPVRPEDREAILVFLKGLSDEA
                     RWLRFFSGFPDLDRAADSAAKVDYHDRFGLLAVVGDIVVGHATYLADGDRAEVGFAVA
                     DAYQGRGLGTIMLGQLAQAAAENDIHTFEAEVMPENRRMLEVFRESGFPIETRTEPGL
                     IKAEFPTSLTREAVERFDSREQVAAMNAMRSFLSPSAVAVIGASRTRGTIGAETFHNL
                     LATGFNGPVYPVNPNAPVVQSVAAYPSIKDAPGPVELAVVVVPAPAVVPVARDCAEAG
                     VRSLVVISSGFAEVGDEGAELQRELLSVCRESGMRLIGPNCMGIMNTDPEVRLDASFA
                     PTFPPHGRVGFLSQSGALGLAVIGRAVELGLGLSSFVSVGNKADVSGNDLLNYWEADE
                     GTDVILLYLESFGNPRRFSRVARRVGRSKPIVAVKSGRTAAGARATSSHTGALIAASD
                     VTVEALFHQTGVIRTDTLEELFDVATLLSSQPLPSGKRVGIITNAGGLGILCADACEA
                     GGLEVPTLSDEAVRKLREFLPAEASVQNPVDMIASASAEDYRMTIEIVAGEGGVDAII
                     VIFIPPLLTRAEDVAHCIRGAADRIDERIPLLTVFMSARGVPDELRTEGRRIPSYSYP
                     EDAARALAHAYRHAEW"
     gene            2030..2398
                     /locus_tag="VF097_00215"
     CDS             2030..2398
                     /locus_tag="VF097_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HEX6261251.1"
                     /translation="MELEEMMKGARDAITVGRVYGEPFEKNGVTILPAASVRGGGGGG
                     SGESPQGEGKGTGGGFGVTARPVGAYVIRGEDVRWEPAVDVTRMALMAQAVGLVALLV
                     IRSIVKSRAKAKARIAKAES"
BASE COUNT          369 a          860 c          868 g          321 t
ORIGIN      
        1 gccactccgc gtgccggtac gcgtgggcca gggcgcgagc cgcgtcctcc ggatacgagt
       61 acgaggggat ccgccgaccc tcggtccgca gctcgtccgg gacgcccctc gccgacatga
      121 agaccgtcag cagggggatc cgctcgtcga tcctgtcggc ggccccgcgg atgcagtggg
      181 cgacgtcctc ggcccgggtg aggagcggag ggatgaagat cacgatgatg gcgtcgacgc
      241 cgccctcgcc cgccacgatc tcgatcgtca tgcgatagtc ctcggcgctg gccgaggcga
      301 tcatgtccac tgggttctgc accgacgcct cggccggaag gaactccctc agcttcctca
      361 cggcctcatc gctgagggtc ggaacctcca agcctcccgc ctcgcacgcg tcggcgcaga
      421 ggatccccag cccgcccgcg ttcgtgatga tgcccacccg cttgccggag ggcagcggct
      481 ggctcgacag cagcgtggcg acgtcgaaca gctcctcgag cgtatcggtc cggatcaccc
      541 cggtctggtg gaacagcgcc tcgaccgtca cgtccgacgc cgcgatcagc gcgccggtgt
      601 gcgaggaggt ggctcgggcc cccgccgccg tccgtccgct cttcaccgcc acgatcggct
      661 tcgagcggcc gacccgcctg gcaacccgcg agaaccgccg agggttcccg aacgactcga
      721 ggtacagcag gatcacgtcg gtgccctcgt cggcctccca gtagttcagg aggtcgttgc
      781 cggagacgtc ggccttgttg cccaccgaga cgaacgacga gagccccagc ccgagctcga
      841 cggctcgccc gatcaccgcc agccccaggg cgccgctctg ggagaggaag cccacgcgcc
      901 cgtgcggtgg gaacgtcggt gcgaacgacg cgtccagcct cacctcgggg tcggtgttca
      961 tgatccccat gcagttcggg ccgatcagcc gcatccccga ctcgcgacag accgagagca
     1021 gctcgcgctg gagctcggcc ccctcgtcgc ccacctcggc gaacccggac gagatcacca
     1081 ccagcgagcg cacgccggcc tcggcgcagt ccctcgccac cggcaccacc gccggcgcgg
     1141 gcaccaccac cacggccagc tcgaccgggc ccggcgcgtc cttgatcgag gggtaggccg
     1201 ccaccgactg cacgacgggc gcgttggggt tcacggggta gacggggccg ttgaacccgg
     1261 tcgcgaggag attgtggaac gtctcggcgc cgatcgtccc tcgggtgcgg gaggccccga
     1321 tgaccgcgac ggccgagggc gagaggaacg agcgcatcgc gttcatcgcc gcgacctgct
     1381 cccggctgtc gaaccgctcc acggcctccc gcgtgagcga ggtggggaac tcggccttga
     1441 tcagcccggg ctcggtgcgg gtctcgatcg ggaagccgct ctcgcggaag acctcgagca
     1501 tccgccggtt ctcgggcatg acctcggctt cgaacgtgtg gatgtcgttc tcggccgcgg
     1561 cctgggccag ctggcccagc atgatcgtgc ccaggccgcg cccctggtag gcgtcggcca
     1621 cggcgaaccc gacctccgcc cggtcgccgt cggcgaggta cgtggcgtgg cccaccacga
     1681 tgtcgcccac cacggccagc agcccgaacc ggtcgtggta gtcgaccttc gccgcgctgt
     1741 cggcggcgcg gtcgaggtcg gggaagccgg agaagaatcg gagccagcgg gcctcgtcgc
     1801 tgagaccctt gaggaagacg aggatcgcct cgcggtcctc gggacggacc ggacggatca
     1861 gaaccgtgga cccgtcccgc aggacgacgt cggcctcgcg atgtgcgggg tactcggtca
     1921 tcgagcccat cctacccgga cgcatccgac cggccccgag ggcgcgggcc cgccgacccc
     1981 cgacgcgaac ggagattcgg cgtacggtgg aacgaaggag gtgggaccca tggaactgga
     2041 ggagatgatg aagggcgccc gcgacgccat caccgtgggc cgcgtgtacg gagagccctt
     2101 cgagaagaac ggcgtgacca tcctccccgc tgcctcggtg cgcggcggcg gaggcggggg
     2161 cagcggcgag agtccgcagg gcgagggcaa aggcaccggc ggcgggttcg gcgtgacggc
     2221 gcgcccggtg ggcgcctacg tgatccgcgg cgaggacgtc cgctgggagc cggcggtgga
     2281 cgtcacgcgg atggcgctca tggcccaggc ggtcgggctc gtggcgctgc tcgtgatccg
     2341 ctcgatcgtg aaatcgaggg cgaaggccaa ggcccggatc gcgaaggccg agagctagcc
     2401 ggaggtccgg atgcgggc
//
LOCUS       DASUTZ010000005         6303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776 k141_1202,
            whole genome shotgun sequence.
ACCESSION   DASUTZ010000005 DASUTZ010000000
VERSION     DASUTZ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..2263)
                     /locus_tag="VF097_00220"
     CDS             complement(74..2263)
                     /locus_tag="VF097_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HEX6261252.1"
                     /translation="MALLRLRVDSGSDIAAGAPLVASVLTEDLETVTEVTVPLGGSRD
                     VEVGAGSYVIRAIAPTGSALTERASVGPEGSVEVELGHIASPHEWLELHALLGDVSAS
                     GTSDELGSGVWLRVWRREADGTWTVEPVSWATIWESEHVVARATGLQPAQRVLQFGGD
                     SIPWRLLALPSGERIEVLLWRADGTPELDAGVDASVVTDRTSGEVLHGYLARGRTEAA
                     GLVAEEMLQGSRPDELESPTSAAVLGYYLVRRGQEERLAGLALGERFPWLPDAWILEG
                     WRALRGGDEEAGRAHLLRAASMGAPLHSEGMRLLSTGLRLLEAASPADADLRSATEGL
                     AGVAAATNWAEPLTTYYGLDPTTPTLAPPTGIPDGPLTRIGPERGVPPPPKRRWGRWV
                     LGAAVVAAVIAGVVWWVTRGGGGLATDPSSLDFGERPIEAAGASEAITLSNDTGAEVL
                     VRDARIRGPDADDFDLTTDTCSGVGLPVGRSCSLVVSFTPTEEGAREARLVIRHEGEG
                     GPIRVPLDGTGVSGEPEEEGSPVIVVDPESITFPADVSEGAADELTITNDGEALLEAA
                     ASVDPADEGIALDADSVAACAEIQPGDSCTISVLFVPFRGSRGVANVVIESNASEQPL
                     QVPVSGTPRAVATPTEVGTTDWEGSEVTVFVRNSGTQAFTPAFDFENERFSATHTCGE
                     LVIGGECAVTIAFDAEEAEDFVGALLIEDGSGEGPVVVPLEYPTPVD"
     gene            complement(2263..3402)
                     /locus_tag="VF097_00225"
     CDS             complement(2263..3402)
                     /locus_tag="VF097_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX6261253.1"
                     /translation="MTTLFDDQADGPRTHLFAVGVGAYRHLAGGDEPLDHLRGVGLGQ
                     LASPPLSARALTDWFLGEMDNPDAPLGSVEMLLSPSHAYDLPGGRSVEVEAATIDNIR
                     RAFDAWFGRCDRHEDNVAIFSFCGHGLMREIVALLAEDFGRSAQRPFEASVDFTMTFE
                     GMATCRARRQLYLVDACRQVMRSTLALQRIDATALAIGTFQREHERDAPILYASAEDD
                     RAYARAGHPTRLTEALLRGLRGLGSEIVGGGWVVTSRRLLEATSDLVVRENLPDAPMQ
                     RPRLGGESAGLALPMHRLPAPPKVPVTIECLPGEAMEVAELSLVPVRPGPSYGRGPSP
                     ERWTLEVEPGDYDAAARFSGGDYPECRKPFVAMPPPPPPFPLEVG"
     gene            complement(3399..4589)
                     /locus_tag="VF097_00230"
     CDS             complement(3399..4589)
                     /locus_tag="VF097_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261254.1"
                     /translation="MPLVFIHGVGTREGPGFEDAVAARDAMFRRFMLARTVADPSAIT
                     ITNPYWGRHGARPAWDHASLPGSPVEALGPEDEDLAMLSAPILEDRPPSDAPLLATAR
                     RSLEEAVDLLWAAAAEISVGASDELARMAARAVDYARHEPDLGWLDEVQTDEEFLEEL
                     ERRVEAWTGDEAPPSGEPEPAYEALGAAESWARIGEAADRIKGFVGRLAGRAVVEVAR
                     APAHRSTALFLGDILVYLRQRGTAAAPGPIVEEVAEALDAARQAVTAEDPRLIVVAHS
                     MGGNIAYDLLTHFRPDLEVDVLVTVGSQVAFFEELKLFAESDDAIPRSGGDRAPKPPN
                     VARWINIFDRNDVLGFVAEGVFSEVEDYEYSTGKGVLFAHTTYFVRPSFHDRLGERVA
                     GSPA"
     gene            complement(4677..>6303)
                     /locus_tag="VF097_00235"
     CDS             complement(4677..>6303)
                     /locus_tag="VF097_00235"
                     /inference="COORDINATES: protein motif:HMM:NF033681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ExeM/NucH family extracellular endonuclease"
                     /protein_id="HEX6261255.1"
                     /translation="TLGRFGEVELAVGGPLDNPTNVVPPGAPAIELQDLNNRSRIQLD
                     DGSNVQNPLPLPPYLGPDGTLRTGDTTPSLSGVLGFAFGVYEVHPTGPVEFDRVQPRP
                     ESPPDVGGSMTVAAFNVLNYFTTLDGQGPICGPLQDQGCRGADNQFELGRQRAKLVAA
                     LAELDADVVGFMEIENHPGDVPTADLVAGINDVLGPGTYDYIATGAVGSDAIRQAIIY
                     KPGRVTPVGDFAILDQSVDPRFLDDFNRPVIAQTFRENGTDDLVTVAVNHLKSKGSDC
                     LDVGDPDLGDGQGNCNLTRLAAAEALVDWLATDPTGSGSAEALIVGDLNSYAQEDPIV
                     AIEAAGYTDLVEEFVGAGWAEGAYSFNFQAQSGYLDHGLASPAAVARVTGAAFWHINA
                     DEPVALDYNDFNQPALFSPDPFASSDHDPVVIGMCETTAPVVDVTVSPDVLWPPDHKY
                     RDVTTSVSVTDADPNAMVQLVSVTSNEPDDGEDDGSTVDDIVIVDDFTFRLRAERSGT
                     GSGREYTITYLVTDACGNSTTASAVVSVPRSQG"
BASE COUNT          893 a         2334 c         2201 g          875 t
ORIGIN      
        1 tcacgctccg cgaggccgca tacctgatcg gggtgggccg ggtcgccgag gccgaggtcg
       61 cccgcgggtt ccgctagtcg accggcgtcg ggtactcgag cgggacgacg accggcccct
      121 cccccgaccc gtcctcgatc agcagcgcgc ccacgaagtc ctcggcttcc tcggcgtcga
      181 aggcgatcgt gaccgcgcac tccccgccga tgacgagctc cccgcaggta tgcgtagcgc
      241 tgaatcgctc gttctcgaag tcgaaggccg gcgtgaaggc ctgggtaccc gagttcctga
      301 cgaacacggt cacctccgac ccttcccagt ccgtcgtccc gacctccgtc ggggtggcga
      361 ccgcccgagg cgtgccggag acgggaacct gcagcggctg ctcgctggcg ttgctctcga
      421 tgaccacgtt ggccaccccc cgggagccgc ggaaggggac gaacagcacg gagatcgtgc
      481 agctgtcgcc gggctggatc tcggcgcacg ccgccaccga gtcggcatcg agggcgatgc
      541 cctcatccgc cgggtccacg ctcgccgcag cctcgaggag ggcctctccg tcgttcgtga
      601 tcgtcagctc gtcggcggcc ccctccgaga cgtcggcggg gaaggtgatg ctctcgggat
      661 ccacgacgat cacggggctg ccttcctcct ccggctcgcc cgacacgccc gtcccgtcga
      721 gcgggacccg gatcgggcct ccctcaccct cgtggcggat caccagcctc gcctcccggg
      781 ccccctcctc cgtcggggtg aaggagacga cgagggagca cgaccgccca acgggcagac
      841 cgacgcccga gcacgtatcg gtggtcaggt cgaagtcgtc ggcgtccggc ccgcggatcc
      901 tcgcatctcg gaccagaacc tccgctcccg tgtcgttcga gagggtgatc gcctccgagg
      961 cacccgcagc ctcgatcggc cgctctccga agtccagcga tgacgggtcg gtggcgaggc
     1021 cgcccccgcc cctggtcacc caccagacga ccccggcgat caccgccgcg accaccgccg
     1081 cgccgaggac ccaccggccc catcggcgtt tgggaggagg gggtacccct cgctccggac
     1141 cgatccgggt gagcggcccg tccgggatgc cggtgggcgg ggcgagcgtg ggggtcgtcg
     1201 ggtcgagccc gtagtacgtc gtgagcggct cggcccagtt cgtggccgcg gccacgccgg
     1261 ccaggccctc ggtcgcgctc cgcaggtcgg cgtcggcggg ggacgccgcc tccaggagcc
     1321 gcaggcccgt ggagaggagc cgcatcccct ccgagtggag aggcgcgccc atcgaggccg
     1381 cgcggaggag gtgggcccga ccggcctcct cgtcccctcc tcggagcgcc cgccagccct
     1441 cgaggatcca ggcgtcgggg agccagggaa agcgctcgcc cagcgccagc ccggccagcc
     1501 gctcctcctg ccctctccgc accaggtagt agccgagaac ggcggccgac gtggggctct
     1561 cgagctcgtc cggccgggat ccctggagca tctcctcggc cacgaggccc gcggcctcgg
     1621 ttcgcccgcg agcgaggtaa ccgtggagca cctcgccgct cgtccgatcg gtgacgaccg
     1681 aggcgtccac accggcgtcg agctccggcg tgccgtcggc ccgccacagc agcacctcga
     1741 tccgctcgcc ggagggcagc gccaagagcc tccacgggat cgagtcgccg ccgaactgca
     1801 ggacccgctg ggcgggctgg aggccggtgg cccgggccac gacgtgctcg ctctcccaga
     1861 tcgtcgccca ggagaccggc tcgaccgtcc acgtcccgtc ggcctcccgg cgccagaccc
     1921 gcagccagac cccggacccg agctcgtccg acgtcccgga agcggacacg tcgccgagca
     1981 gcgcgtgcag ctcgagccac tcgtggggcg aggcgatgtg tcccagctcg acctcgaccg
     2041 acccctccgg gcccaccgac gcccgctcgg tgagcgccga gccggtcgga gcgatcgccc
     2101 tgatcacgta ggagcccgcc cccacctcga cgtcgcggct cccgccgagc ggcaccgtga
     2161 cctccgtcac cgtctccagg tcctcggtca ggacggaagc gacgagcgga gcgccggcgg
     2221 cgatgtcgga cccgctgtcg acccggaggc ggaggagcgc catcagccca cctccaatgg
     2281 gaagggtggt ggtggtggtg gcatggccac gaacggcttc cggcactccg ggtagtcgcc
     2341 gccggagaag cgcgcggcgg cgtcgtagtc tcccggctcc acctcaagcg tccaccgctc
     2401 gggcgacggc ccgcggccgt acgagggccc gggccgcacc ggcacgaggg aaagctcggc
     2461 cacctccatc gcctcgccgg ggaggcactc gatcgtgacc ggcaccttgg gcggcgcggg
     2521 gaggcgatgc atcggcaggg cgaggccggc cgactccccg ccgaggcggg ggcgctgcat
     2581 cggggcgtcg ggcaggttct cccggacgac gaggtccgag gtcgcctcga gcagcctccg
     2641 ggacgtcacg acccatccgc cgccgacgat ctcgctcccc aggccgcgga ggccgcggag
     2701 cagcgcttcc gtcagccggg tgggatgccc cgccctcgcg taggcgcgat cgtcctccgc
     2761 ggaggcgtag aggatcgggg cgtctcgctc gtgctcgcgc tggaaagtgc cgatcgcgag
     2821 ggccgtggcg tcgatccgct ggagggccag ggtggagcgc atcacctgcc ggcacgcgtc
     2881 caccaggtag agctggcgcc gggctcggca cgtggccatc ccctcgaagg tcatcgtgaa
     2941 gtcgaccgag gcctcgaagg gacgctgggc gctccgtccg aagtcctccg cgagcagcgc
     3001 cacgatctct cgcatgaggc cgtggccgca gaaggagaag atcgcaacgt tgtcctcgtg
     3061 acgatcgcat cggccgaacc acgcgtcgaa tgcccggcgg atgttgtcga tcgtggcggc
     3121 ctccacctcg accgagcggc cccccggaag gtcgtaggcg tgggagggcg acagcagcat
     3181 ctctacgctt cccagaggag catccggatt gtccatctcg ccgaggaacc agtcggtcag
     3241 cgcgcgggcg gacaggggcg gcgaggcgag ctggcccagg cccacgcccc ggagatggtc
     3301 gaggggctcg tcaccacctg ccaggtgtcg gtacgctccc acccccaccg cgaacagatg
     3361 ggtccgcggg ccgtcggcct ggtcgtcgaa gagcgtcgtc atgccggcga ccccgccacg
     3421 cgctcgccga ggcggtcgtg gaagctgggc cgcacaaagt acgtggtgtg ggcgaagagg
     3481 acgcccttcc ccgtcgagta ctcgtagtcc tcgacctcgc tgaagacccc ctccgccacg
     3541 aacccgagca cgtcattgcg atcgaagatg ttgatccagc gcgcgacgtt cgggggcttc
     3601 ggcgcgcgat cgcccccgct cctcgggatg gcgtcgtcgc tctcggcgaa cagcttcagc
     3661 tcctcgaaga aggcgacctg cgatcccacc gtcaccagca cgtccacctc aaggtccggg
     3721 cgaaagtggg tgaggaggtc gtaggcgatg ttccccccca tgctgtgcgc gaccacgatc
     3781 agacgcggat cctcggcggt cacggcttgc cgcgccgcgt ccagcgcctc cgccacctcc
     3841 tcgacgatgg gccccggcgc cgccgccgtc ccccgctgac ggagatacac gaggatgtcg
     3901 ccgaggaaga gcgcggtgct ccggtgggcg ggggccctgg caacctccac cacggcccga
     3961 cccgcgagcc gtccgacgaa ccccttgatc cggtcggcgg cctcgccgat ccgggcccag
     4021 ctctcggcgg cgcccagcgc ctcgtaggcg ggctccggct cacccgaggg aggcgcctcg
     4081 tccccggtcc acgcctccac gcggcgctcc agctcctcca ggaactcctc gtccgtctgc
     4141 acttcgtcca gccacccgag gtccggctcg tgcctggcgt agtccacggc tcgggccgcc
     4201 atccgagcga gctcgtcgga cgcgcccacg gagatctcgg ccgcagccgc ccacagcaga
     4261 tccaccgcct cctcgagcga ccgccgagcc gtggccagca ggggggcgtc gctcggcggg
     4321 cggtcctcca ggatcggggc gctcagcatg gcgagatcct cgtcctcggg gcccagcgcc
     4381 tcgacggggg accccgggag ggatgcgtga tcccacgcgg gacgggcgcc gtggcgtccc
     4441 cagtacgggt tcgtgatcgt gatggcagag ggatcggcca ccgttcgggc gagcatgaat
     4501 cgccggaaca tggcgtccct ggcggcgacc gcgtcctcga accccgggcc ctcccgggtc
     4561 cccacaccgt ggatgaacac caggggcatg acgaacctcc tcgagtccgg gaacagtaga
     4621 gcggccgccc gccggacacc atcggccgtt cggcctccgc cgaacgcgct acgggctcag
     4681 ccctggcttc gaggaacgga gaccaccgcg gacgccgtcg tgctgttgcc gcaggcgtcg
     4741 gtcacgaggt aggtgatcgt gtactcccgc cccgagcccg tccccgagcg ctcggccctg
     4801 agccggaagg tgaagtcgtc cacgatcacg atgtcgtcta cggtgctccc gtcgtcctcg
     4861 ccgtcgtcgg gctcgttcga cgtcaccgag accagctgga ccatcgcgtt cggatcggcg
     4921 tcggtcaccg agaccgacgt ggtcacgtca cggtacttgt ggtcgggcgg ccacagcacg
     4981 tccggcgaga ccgtcacgtc cacgaccggg gccgtcgtct cgcacatccc gattacgacg
     5041 gggtcgtggt cgctcgaggc gaacgggtcg gggctgaaca gcgcgggctg gttgaagtcg
     5101 ttgtagtcga gggccaccgg ctcgtcggcg ttgatgtgcc agaacgcggc cccggtcacc
     5161 ctggccaccg ctgcggggct ggccagcccg tgatccaggt accccgactg cgcctggaag
     5221 ttgaaggagt aggctccctc ggcccagccc gcgcccacga actcctcgac cagatccgtg
     5281 tagcccgcgg cctcgatcgc cacgatcgga tcctcctgag cgtacgagtt gagatccccc
     5341 acgatcagcg cctcggcgct cccgcttccc gtggggtcgg tggccagcca gtccacgagc
     5401 gcctcagccg cggccagccg cgtcaggttg cagttgccct ggccgtcgcc gaggtcgggg
     5461 tcacccacgt cgaggcagtc agagcccttc gacttgaggt ggttgacggc caccgtcacc
     5521 aggtcgtcgg tgccgttctc ccggaaggtc tgcgcgatca cggggcggtt gaagtcgtcg
     5581 aggaaccggg gatccacgct ctgatcgagg atcgcgaagt cacccacggg cgtgacccgg
     5641 cccggcttgt agatgatcgc ctgacggatc gcgtccgagc cgaccgcccc cgtcgcgatg
     5701 tagtcgtagg tgcccggacc gagcacgtcg ttgatccctg ccacgaggtc cgccgtgggc
     5761 acgtcgcccg ggtggttctc gatctccatg aagccgacca cgtccgcgtc gagctcggcc
     5821 agcgccgcca cgagcttcgc ccgctgccgc ccgagctcga actggttgtc ggcccccctg
     5881 cagccctgat cctggagcgg cccgcagatc ggcccctggc cgtcgagcgt cgtgaagtag
     5941 ttgaggacgt tgaaggccgc gacggtcatc gagccgccga catcgggggg gctctccggg
     6001 cggggctgca cgcggtcgaa ctccacgggc ccggtgggat ggacctcgta cacgccgaag
     6061 gcgaagccga ggacgccgga gagcgacggg gtcgtgtcgc cggtgcggag cgtcccgtcg
     6121 ggcccgaggt acggcgggag cgggagcggg ttctggacgt tgctgccgtc gtcgagctgg
     6181 atcctgctcc ggttgttcag gtcctgaagc tcgatggcgg gcgcccccgg cggcacgacg
     6241 ttggtcgggt tgtcgagcgg ccctcccacc gccagctcca cctcgccgaa ccggcccagc
     6301 gtg
//
LOCUS       DASUTZ010000006         5603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_519395, whole genome shotgun sequence.
ACCESSION   DASUTZ010000006 DASUTZ010000000
VERSION     DASUTZ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519395"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..140)
                     /locus_tag="VF097_00240"
     tRNA            complement(67..140)
                     /locus_tag="VF097_00240"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(104..106),aa:Thr,seq:ggt)
     gene            168..656
                     /locus_tag="VF097_00245"
     CDS             168..656
                     /locus_tag="VF097_00245"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX6261256.1"
                     /translation="MLHRPMILGVCLLLAACGSEETTRPDPSPTGEDPPFATDAALVI
                     EPAEGDPVTLRVAIADTDEERTRGLMKVEEMPDDVGMVFLHEEPTESDFWMKNTLIPL
                     TVAVWDESMTIRQIVDMEPCEEDPCRIYDITVAWVGAVEVNQGVLGRMGVSVGDRVRL
                     ED"
     gene            793..2367
                     /locus_tag="VF097_00250"
     CDS             793..2367
                     /locus_tag="VF097_00250"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX6261257.1"
                     /translation="MAGDRRRRRQKDTWRSHGPASFLVRALVLLVPVGAGVATAILVG
                     SVLPLPAAGADDVVWWVLVLGSSTLAALLTDHLARKLLPLAVLLKLTLVFPDRAPSRF
                     ALARAAGSVRSLERRVREARERGVGDVPARAAETVLVLIAALGAHDRRTRGHSERVRA
                     FTDVLSDELKLPPGARARLRWAALLHDIGKLEVTADILNKPGELDEREWDLIRDHPEA
                     GARLVGPLLPWLGEWGRAIPEHHERYNGSGYPQGISGENISLAARITSIADAYETMTA
                     ARTYKPSLGVRGARRELARHAGSQFDPTLVRAFLNVSLGRLLWISGPLTWIAQVPIVR
                     GLARSGEHQPVQAAASWALIRTIAGVLALGMTGAIAPRSSEPPNVAGAGPALEQEAED
                     RGENAARSRDVDEAVLQALDYRFALRRPVVAAFEEGAGGVEAPSDQAGGGPDDGSDEG
                     PGDPAAQPPEEGPGEPPGGGPPGGGPPGGGPGQPGEPPVEGPPGKKGRRFDPPPLKPP
                     PDPPPADPPPVDPPPG"
     gene            complement(2397..2481)
                     /locus_tag="VF097_00255"
     tRNA            complement(2397..2481)
                     /locus_tag="VF097_00255"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2445..2447),aa:Tyr,seq:gta)
     gene            2661..3158
                     /locus_tag="VF097_00260"
     CDS             2661..3158
                     /locus_tag="VF097_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEX6261258.1"
                     /translation="MADKSSFDVVSEVDLAEVTNAVDQARREVATRFDFKGTDTSIEQ
                     DEDLVEVRSSTEERLKAAVEVFKDKCVKRKISLKALSEGPVLPGAKATYKQAVHINRG
                     IPQEKAREMVKFIKDLKLKKVQTAIQGDQLRVSAPKKDDLQEVIGALREADFGIPLQF
                     RNYRG"
     gene            complement(3155..4372)
                     /locus_tag="VF097_00265"
     CDS             complement(3155..4372)
                     /locus_tag="VF097_00265"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX6261259.1"
                     /translation="MTTRRLAILTGLLVSLQALIPLSFAGAQVDPCSPVVIPGLCNPE
                     PSPKPKPKPKPKPDDDRRDDRRGRGGGIGGGGGGGAGSGVITRRGGGQPGSPGFVLGG
                     PENTADLIETLTRLTRFGVPLRRSVASVVGPFPVAGLAWWADDWHACRDGCTRLHEGL
                     DIFAESGTPLVAAADGVVTQKGEGTLSGLHVEIEDAEGTQYFYAHLSGWADGLLIGQR
                     VRMGQVLGYVGNTGNAVNTPPHVHFEYQPNGVPQPPKPVVDRWLRIAEARAADLVRQY
                     AGEEPIDLVSLDTFRLTRLFDLAGGGVDLSPGSARLLVLAGLQPAASSLEVTRRTIDE
                     MAWEIDWGEHVRARLDELIGEYEKYAGLDGLGGPPFLTVLNRERLDEAGLDEQDLDQH
                     ELDERVQPAEVGD"
     gene            complement(4431..5279)
                     /locus_tag="VF097_00270"
     CDS             complement(4431..5279)
                     /locus_tag="VF097_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013018619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEX6261260.1"
                     /translation="MLGINHVKSWLLIGGLAGLFVAVGAMVGGGRGATIALVLAVAFN
                     GIMYWSSDRIAIAATRSKPVTREQAPELYAIVEELAMQAKAPMPRIFVSEMAQPNAFA
                     TGRSPEHAAVSVTRGILQILDRRELRGVLAHELSHVLNRDILVSSIAGAIASAFTYLA
                     YLAFFIGGDDDSPLSGIGFLLLWLVGPLAAGLIQMAVSRSREYQADESGALLSQDPEA
                     LASALAKLEQASRVVPARVNPAQAHVFIVNPLRGQRGGGIASLFSTHPPTEERVRRLE
                     ELGTRI"
     gene            complement(5333..>5603)
                     /locus_tag="VF097_00275"
     CDS             complement(5333..>5603)
                     /locus_tag="VF097_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261261.1"
                     /translation="FWAKFQIFAVAIVADQAYLAVAVVVNSVISLYYYFSIVRQMFFV
                     EGLEERPFRASALVTGVVALAAVAIVVVGIFPDLFAGFPDRLSVP"
BASE COUNT          867 a         1966 c         1978 g          792 t
ORIGIN      
        1 ccgcttgctc tgcctctgag ctaccccgcc gcgcccgtct cagtctagca accgtctcgt
       61 gccgggagct ggaggtggga ctcgaaccca cgacccccgc cttaccatgg cggtgctctg
      121 ccgagctgag ctactccagc ggaaccgatg ccatgatagt ggcagacatg ctgcatcgcc
      181 caatgatcct cggcgtctgc ctcctgctgg ccgcgtgcgg gagcgaggag acgacacggc
      241 ccgacccctc ccccaccggg gaggaccccc ctttcgccac ggacgcggcg ctcgtgatcg
      301 agccggccga gggagacccg gtgacgctcc gggtcgcgat cgccgacacg gacgaggagc
      361 ggacccgggg cctcatgaag gtcgaggaga tgcccgacga cgtgggcatg gtcttcctcc
      421 acgaggagcc gaccgagagc gacttctgga tgaagaacac cctgatcccc ctgacggtgg
      481 cggtctggga cgagtccatg acgatccgcc agatcgtgga catggagccg tgcgaggagg
      541 acccctgccg gatctatgac atcaccgtgg cctgggtggg ggcggttgag gtcaatcagg
      601 gggtgctcgg acggatgggc gtgtccgtcg gcgatcgcgt gcgcctcgag gattagcgaa
      661 ccgtcgggct tcgctcgacg tgcatgctgc gggtcggcgg cgggccgggc cttctgtgca
      721 tacgccatcg actgtacatc gaacaacgga taccaaacct ccggcggacc gggccacagt
      781 gggaccctga tcatggccgg ggatcggcgt cgtcgcaggc agaaggacac atggcggagc
      841 cacgggccag cgagcttcct cgttcgcgcc ctcgtgctcc tcgtcccggt gggcgccggt
      901 gtggccaccg ccatcctggt cggctccgtg ctcccgctgc ccgcagccgg tgccgatgac
      961 gtcgtctggt gggtcctggt cctcggctct tcgacgctgg cggctctcct gacggaccac
     1021 ctcgcgcgca aactcctgcc tctggccgtg ctcctgaagc tcaccctcgt gttccccgac
     1081 cgcgccccgt cccgcttcgc gctggctcgg gccgcgggca gcgtccgctc cctggagagg
     1141 cgcgtccgag aggctcgcga gcggggggtg ggtgacgttc cggccagggc cgccgagacg
     1201 gttctggtgc tgatcgcggc gctgggagcg cacgaccggc ggacgcgggg ccactcggag
     1261 cgggtgcgcg ccttcacgga cgtgctctct gacgagctca agctgccgcc gggcgctcga
     1321 gctcgactgc gctgggccgc cctgctccac gatatcggga agctcgaggt caccgcggac
     1381 atcctcaaca agcccggtga gctcgatgag cgagagtggg acctgatccg ggaccacccc
     1441 gaggccggcg cgcgactcgt cggaccgctg ctcccgtggc tgggggaatg gggccgcgcc
     1501 atccccgagc accacgagcg gtacaacggg agcggctatc cccaggggat atccggggag
     1561 aacatcagcc tggccgcacg catcacgtcg atcgccgacg cctacgagac catgacagcc
     1621 gcccgtacct acaagccgtc cctgggtgtc cgcggcgcgc ggcgagagct ggcgaggcac
     1681 gccggctccc agttcgatcc cacgctcgtg cgggcgttcc tgaacgtgtc tctcggacgc
     1741 ctgctgtgga tctcgggacc gctgacctgg atagcccagg tcccgatcgt ccgagggctc
     1801 gccaggtccg gggagcacca gccggtacag gccgcggcct cctgggccct gatccgcacg
     1861 atcgcggggg tgctcgccct ggggatgacc ggggccatcg cccctcgatc gagcgagccg
     1921 ccgaacgtcg cgggtgccgg ccccgcgctc gaacaagagg cggaggatcg gggggagaat
     1981 gcggcccgct cgagagatgt ggacgaggcc gtcctccagg ctctcgacta ccgcttcgcc
     2041 cttcggagac ccgtcgtcgc tgcgttcgag gagggagccg gcggtgtgga ggcgccgagc
     2101 gaccaagcgg gcggtggacc ggacgacggg agcgatgaag ggcccgggga tccggcagct
     2161 cagcccccgg aggaggggcc cggggagcct cccggtggcg ggcctcccgg tggcgggcct
     2221 cccggtggcg gacccggtca gccgggcgag cccccggtcg agggcccacc cgggaagaag
     2281 ggccggcgct tcgacccacc cccgctgaag ccaccgccgg acccgccgcc cgcggatccg
     2341 ccaccggtgg acccgccgcc gggctagggg tggctctcgc cacgaggagc gggtggtggt
     2401 ggggggagga ggattcgaac ctccgtaggc tatgccggcg actttacagg ccgctccctt
     2461 tggccgctcg ggcatccccc cgcgctggga tcaagggtag ccgatggcga gaggccacgt
     2521 ccctcgtcct ccgaacgaac cattgtccga tggaggccgg agcctggtgg atgcacctct
     2581 gccccacgaa cgagacggtc gtcgccggcg gcgccccgcg tccccacagg agcagcgccc
     2641 tctgtcgtag actcgcggcc atggccgaca agagctcgtt cgatgtcgtg tcggaggtgg
     2701 acctggccga ggtgaccaac gcggtcgacc aggctcgccg cgaagtagcg acgcgcttcg
     2761 acttcaaggg gaccgacacc tcgatcgagc aggacgagga tctggtcgag gtccgaagct
     2821 ccaccgagga gcgcctgaag gccgcggtcg aggtcttcaa ggacaagtgc gtgaagcgga
     2881 agatctctct gaaggcgcta tccgaggggc cggtgctccc cggagcgaag gcgacgtaca
     2941 agcaggccgt ccacatcaac cggggcatcc cccaggagaa ggcccgcgag atggtgaagt
     3001 tcatcaagga tctgaagctg aagaaggtcc agacggcgat ccagggggac cagctccggg
     3061 tctcggcccc caagaaggac gacctccaag aggtgatcgg tgccctgcgc gaggccgact
     3121 tcgggatccc gctgcagttc cggaactacc ggggttagtc ccccacctcc gcgggctgga
     3181 cccgctcgtc gagttcgtgc tgatcgagat cctgctcgtc gaggccggcc tcgtccagac
     3241 gctcccggtt caggaccgtg aggaagggcg gcccccccag accgtccagg ccggcgtact
     3301 tctcgtactc gccgatcagc tcgtcgagcc gggctcggac gtgctcgccc cagtcgatct
     3361 cccaggccat ctcgtcgatc gtccgccgcg tgacctcgag cgacgaggcc gccggctgca
     3421 gcccggccag gacgaggagc cgcgcgctcc ccgggctcag gtcgaccccg cccccggcca
     3481 ggtcgaagag tcgggtgagg cggaaggtgt cgaggctcac cagatcgatc ggctcctccc
     3541 ccgcgtactg gcggacgagg tcggcggccc gcgcctccgc gatccggagc cagcggtcaa
     3601 ccacgggctt ggggggctga ggcaccccgt tcggctgata ctcgaagtgg acgtgaggcg
     3661 gggtgttcac ggcgttcccc gtgttcccca cgtagcccag gacctgtccc atgcggacgc
     3721 gctggccgat cagcagcccg tcggcccacc cggagaggtg cgcgtagaag tactgcgtcc
     3781 cctccgcgtc ctcgatctcg acatggaggc ccgagagcgt cccctcgccc ttctgcgtga
     3841 cgacgccgtc cgccgcggcg acgagcggcg tcccggactc cgcgaagatg tcgaggccct
     3901 cgtggagccg cgtgcagccg tcccggcagg cgtgccagtc gtcggcccac caggcgagcc
     3961 ccgccacggg gaagggcccc acgaccgagg cgaccgacct ccgcagcggg acgccgaacc
     4021 tcgtgagcct ggtgagggtc tcgatgagat cggctgtgtt ctcggggccg ccgaggacga
     4081 acccggggct tcccggctgc ccgccgccgc gacgcgtgat gacgccggag ccagctccgc
     4141 cccctccgcc gccgccgatc ccgccgcccc tcccccgccg atcgtctcgc cggtcgtcgt
     4201 cgggcttcgg cttgggcttg ggcttcggct tgggcgaggg ctcggggttg cagaggccgg
     4261 ggatcacgac gggcgagcac ggatcgacct gggcgccggc gaacgacaag gggatgaggg
     4321 cctgcagaga caccagcagg cccgtgagga tggcgagcct cctcgtcgtc acgctcgtgc
     4381 tcctcccctc tcccccgcat cctgccggac ggggcgcgat tggtacaaca tcagatccgc
     4441 gtgccgagct cctcgagacg ccggacgcgc tcctcggtgg gcgggtgggt cgagaacagt
     4501 gaggcgatgc ctccaccgcg ctgcccccgg agcgggttca cgatgaacac gtgggcctgg
     4561 gccgggttca cccgggcggg gacgacgcgg gatgcctgct ccagcttcgc gagggcgctg
     4621 gccagggcct cgggatcctg cgacagcaga gccccagact cgtcggcctg gtactcgcgc
     4681 gacctcgaga ccgccatctg gatcaggccc gccgcgagcg ggcccacgag ccacagcagg
     4741 aggaagccga tccctgagag cgggctgtcg tcgtccccgc cgatgaagaa cgcgaggtac
     4801 gcgaggtagg tgaacgcgga ggcgatcgcc ccggcgatgg aggagacgag gatgtcccgg
     4861 ttcaggacgt gcgagagctc gtgggccagc actccgcgca gctcccggcg atcgaggatc
     4921 tggaggatgc cacgggtcac gctcacggcc gcgtgctcgg gactccgacc ggtcgcgaac
     4981 gcgttgggct gggccatctc gctcacgaag atgcggggca tgggggcctt cgcctgcatc
     5041 gccagctcct cgacgatggc gtagagctcc ggcgcctgct cgcgcgtcac cggcttcgac
     5101 ctggtggcgg cgatcgcgat cctgtcgctc gaccagtaca tgatcccgtt gaaggcgacg
     5161 gccagcacca gcgcgatcgt cgctccgcgg ccgcctccga ccatcgcccc cacggcgacg
     5221 aacagcccgg cgaggcctcc gatcaggagc cagctcttga catggttgat ccccagcatc
     5281 tccgaacctc ctggcacgat tgtagggact cccccggcct gcgcggggcc cgctatggga
     5341 cggagagccg gtcggggaaa ccggcgaaga ggtcggggaa tatccccacg accacgatcg
     5401 ccacggcggc gagggccacc acgcccgtca ccaaggcgga ggcccggaag ggccgctcct
     5461 caaggccctc cacgaagaac atctgcctga cgatcgagaa gtagtagtac aggctgatca
     5521 ccgagttcac gaccacggcc acggccaggt aggcctggtc cgccacgatc gccaccgcga
     5581 agatctggaa cttggcccag aac
//
LOCUS       DASUTZ010000007         4108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_778527, whole genome shotgun sequence.
ACCESSION   DASUTZ010000007 DASUTZ010000000
VERSION     DASUTZ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778527"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..503)
                     /locus_tag="VF097_00280"
     CDS             complement(90..503)
                     /locus_tag="VF097_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6261262.1"
                     /translation="MDYKLELVVLPVSDVDRAKAFYAERAGFRLDVDTTPAEGFRVVQ
                     LTPPGSACSITFGRGLTDAEPGAYKGLHLVVSDIVAARQELVGRGVEVREIRHMSSGE
                     WKPGPDPDRQDYNSFADFQDPDGNLWLLQEVPSRD"
     gene            603..1409
                     /locus_tag="VF097_00285"
     CDS             603..1409
                     /locus_tag="VF097_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014688983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX6261263.1"
                     /translation="MNDPRDAFRELHEQGLFVMPNPWDVGSARLLAWLGFRALATTSS
                     GHAASLGRLDQHVDRDEMLAHAAAIAEAVDVPVSVDAERCFADDPEGVAETVRLIAGT
                     GAAGCSVEDYDPATGAIDPLEVAVDRVAAAAGAARPTGLVLTARAENHLYGLGDLDDT
                     IARLRAYRDAGAEVVYAPGLLDLDEIARVVREGEAPVNVLARPRSPSIPELASVGVRR
                     VSTGGALAMAAYGSLVSAAGELLDAGTSSYLAGAVGDDVLEAAFRQRKRG"
     gene            complement(1387..2169)
                     /locus_tag="VF097_00290"
     CDS             complement(1387..2169)
                     /locus_tag="VF097_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261264.1"
                     /translation="MAHGDGSVRDARSTGRLRAFRVIAMVMAVSAIAFGLFTAIGAVS
                     EAQEIHAYHNVIVASLLLVLSAPPVVAAARGPERAAPYLVHLVMLGVAAVATMAIALM
                     VDVFTLPFVALAVVLILLRVPREPAVGAGRPSVPLAIVVAVAAVPLLAYAIDQAGMQR
                     ADASSEHAQLNHWVEVAFYAAAIPLLGALAAIRPRVFRPAGLTAGAALAILGGGSLLL
                     EGYASSPDARWAWAALAGGLVLLGLVEWEARRGASLNRVSAA"
     gene            2264..2758
                     /locus_tag="VF097_00295"
     CDS             2264..2758
                     /locus_tag="VF097_00295"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEX6261265.1"
                     /translation="MAVATMRAEGRVLRLLHLASRLALGGLLLWAGLTKVGDVQGMTL
                     SIDSYQILPGWSVRPLAAALPWIEVALGAFLVLGIFVRFSAVATGVLLVAFLIGMAQA
                     KARGLAIDCGCFGAGGPGEGVSWFDILRDVALIGVAGLVLLLPADPWGLDVRLDRPRH
                     GEET"
     gene            2758..3411
                     /locus_tag="VF097_00300"
     CDS             2758..3411
                     /locus_tag="VF097_00300"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX6261266.1"
                     /translation="MAQRTMTKKERREAARTARLEAQQRARRKRLIRSWGIPLAVIAA
                     IALVAFITTMGDEGGPEVGEVEISGPARQAALEAGEPFPEFSATELEGGEVSWAPGEP
                     SLIAIWAPWCPICQEEIPMIDRLADEYLEIEMVTVATAVDDRPGPTVSGFVEDEGLSL
                     PVALDDRAGTLARAMGIRGFPTLYLVDAGGAVVAAAEGRVGEEALRDALDQLTGPAG"
BASE COUNT          552 a         1429 c         1546 g          581 t
ORIGIN      
        1 ccgaagcaga ccggcgcgtt catcgagcgc cgcacgctgc acggcggcga cgggagcgac
       61 cacgacccgg cgtgggtcgt gctctcgatc tagtcgcggc tggggacctc ctggaggagc
      121 cagaggttgc cgtcggggtc ctggaagtcc gcgaacgagt tgtagtcctg acgatcgggg
      181 tcgggccccg gcttccactc gccagacgac atgtggcgga tctctctcac ctcgacgccc
      241 cgtcccacga gctcctggcg ggcggcgacg atgtccgaga cgacgaggtg cagtcccttg
      301 taggcccccg gctcggcgtc ggtcagcccc ctgccgaacg tgatcgagca ggccgaaccc
      361 gggggtgtga gctggacgac ccggaacccc tcggccgggg tcgtgtccac gtcgaggcgg
      421 aagccggctc gctcggcgta gaaggccttg gctcgatcca cgtcgctgac cgggagcact
      481 acgagctcga gcttgtagtc catcggatcc tccttctggg tctcgtcatc tcgaccctac
      541 ggcgcgccgc gcctcatcgc tcctcgatac gatcgaccat ccgctcacca aggagacgat
      601 cgatgaacga tccgcgtgac gccttccggg agctccatga gcaggggctg ttcgtgatgc
      661 ccaacccctg ggacgtgggg tcggctcgtc tgctcgcctg gctcggcttc cgggccctgg
      721 ccacgacgag ctccgggcac gccgcctcgc tcgggcggct ggaccaacac gtcgatcgcg
      781 atgagatgct ggcccacgcc gcggcaatcg ccgaggcggt ggacgtcccg gtgagcgtgg
      841 acgccgagcg atgcttcgcc gacgaccccg agggtgtggc cgagaccgtg cggctcatcg
      901 cggggaccgg cgccgccggc tgctcggttg aggactacga tcccgccacc ggagccatcg
      961 acccgctcga ggtggcggtg gaccgggtgg ccgccgcggc gggcgccgcg cgccccaccg
     1021 gcctcgtgct cacggcccgg gccgagaacc acctctacgg actgggggac ctggacgaca
     1081 cgatcgccag gctgcgggcg taccgggacg cgggcgcgga ggtcgtctac gctcccgggc
     1141 tcctcgacct ggacgagatc gctcgggtcg tgcgcgaagg ggaggcgccg gtcaacgtgc
     1201 tcgcccgacc caggagtccc tcgatccccg agctcgcgtc ggtcggcgtg cgcagggtct
     1261 ccaccggcgg ggcgctggcg atggccgcct acggcagcct ggtctcggcc gccggcgagc
     1321 tcctcgacgc cggcacgtcg tcgtacctgg ccggggcggt gggcgacgac gtcctggagg
     1381 cggcgttcag gcagcggaaa cgcggttgag gctcgccccg cgacgcgcct cccactccac
     1441 aaggcccagg aggacgaggc ccccggccag tgccgcccag gcccaccgtg cgtccgggga
     1501 cgaggcgtat ccctccagga ggagcgagcc gccccccagg atggccagcg cggctcccgc
     1561 cgtcaggccg gccggccgga agacccgggg acggatcgcc gccagcgcgc cgaggagggg
     1621 gatggccgcc gcgtagaagg cgacctcgac ccagtggttc agctgcgcgt gctcgctgga
     1681 ggcgtccgct cgctgcatgc cggcctggtc gatcgcgtac gcgagcaggg gcacggcggc
     1741 cacggccacc acgatcgcga gggggacgct ggggcgtccg gctcccaccg ccggctcgcg
     1801 cggtacccgc aggaggatga gcaccaccgc cagcgccacg aaggggagcg tgaagacgtc
     1861 caccatgaga gcgatcgcca tcgtggccac ggccgcgacc ccaagcatca ccaggtggac
     1921 caggtaaggc gccgctctct ccgggccgcg agcggccgcc accacgggcg gcgcggagag
     1981 caccaggagc agcgaggcca cgatcacgtt gtggtaggcg tggatctcct gcgcctcgct
     2041 gacggccccg atcgccgtga agagcccgaa ggcgatggcc gagacggcca tgaccatcgc
     2101 gatcacgcgg aaggcgcgga gccgcccggt cgatcgggcg tcgcgcaccg agccgtcgcc
     2161 gtgcgccacg gacctcagcg tacaccgggg gccggtggga ccgcccgcgg ggggacttcc
     2221 ggcccgggtc gggaccccgc gggccgccga tatccttccc catatggcgg tcgcgacgat
     2281 gcgcgccgag gggagggtcc tccggctcct ccacctcgcc tccaggctgg cgctgggcgg
     2341 gctcctgctc tgggcggggc tgaccaaggt gggggacgtg caggggatga ccctctcgat
     2401 cgactcctac cagatcctgc cggggtggtc ggtgcggccg ctggccgcgg ccctcccgtg
     2461 gatcgaggtc gcgctcggcg cgttcctggt gctcgggatc ttcgttcgct tctcggcggt
     2521 cgcgaccggg gtgctgctcg tggcgttcct gatcgggatg gcgcaggcga aggcccgcgg
     2581 cctcgccatc gactgcggct gcttcggggc ggggggtccg ggcgagggcg tgagctggtt
     2641 cgacatcctg cgggacgtgg cgctcatcgg cgtggcgggg ctggtgctgc tcctcccggc
     2701 cgacccgtgg gggctggacg tgcggctgga ccggccgcgg cacggagagg agacgtgatg
     2761 gctcagcgca ccatgaccaa gaaggagcgc cgggaggccg cgaggacggc gaggctcgag
     2821 gctcagcagc gggccaggcg gaagcgcctg atccgctcgt gggggatccc cctggccgtg
     2881 atcgccgcca tcgcgctcgt ggccttcatc acgacgatgg gggacgaggg cggtccggag
     2941 gtcggcgagg tggagatctc cggcccggcc cggcaggcgg cgctggaggc cggcgagccg
     3001 ttccccgagt tctccgcgac cgagctcgag gggggcgagg tgtcgtgggc gcccggcgag
     3061 ccctcgctga tcgcgatctg ggccccctgg tgcccgatct gccaggagga gatccccatg
     3121 atcgaccgcc tggcggacga gtaccttgag atcgagatgg tgaccgtggc caccgcggtc
     3181 gacgaccggc ccgggccgac ggtgtcgggg ttcgtggagg atgaagggct cagcctcccg
     3241 gtcgcgctcg acgaccgggc agggacgctg gcgagggcca tgggcatccg ggggttcccg
     3301 acgctctacc tcgttgacgc tgggggagcg gtcgtggcgg ccgccgaggg ccgggtcggg
     3361 gaggaggctc ttcgggatgc cctcgaccag ctgaccgggc cggcgggctg agcgaggggc
     3421 gggcgccccg ggccgcaggc ccggggcgcc cgcgaggtca ggtcccagtt gaggatcttc
     3481 gatcgcacgt tcctcgggat cccggtcagc agcctcctgt tcagcgtccg cttgttgaac
     3541 ttcttcgccg cgttgatcgt ggcctcgttg tcgagcctga tcggggtgac agccggagga
     3601 ccctgtccct gcgcgtgggg ttcttgaacc ggatcagcga gcgcagggtg gggctcgtct
     3661 ggagggcccg ctcgatccgg gtcaccttca ccgtgctgaa cctctgtggc cccaccttga
     3721 gctgctgccc cgaagggttc cccgtctcga tacccgcgcc gaaggtcttc gcgttcacga
     3781 gcaggaggag ccccccgccg aagacgtcgt tgctgaagtc gttccacgtt gaccgcgggc
     3841 gagaagccct cgcgggcgaa gcagggcccg ccgctgccat ggtcgtcgtt cccatccgcg
     3901 tcccactcgg cgccctgagg ttccgtgagg accacgtcgt cgatgggcgt cggcgccgtc
     3961 gacgcgcccg aggctacgag cgtgagcccc gccacgaggg ggccgtgagg actcccccga
     4021 acggatgagg tcgaacggcc tacccgtctc gccagcgcac ggggacgacc cgtacgggct
     4081 ccgagatccc tttcagggag accgttcg
//
LOCUS       DASUTZ010000008         4732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1296908, whole genome shotgun sequence.
ACCESSION   DASUTZ010000008 DASUTZ010000000
VERSION     DASUTZ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296908"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VF097_00305"
     CDS             <1..579
                     /locus_tag="VF097_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEX6261267.1"
                     /translation="TERGRERFRGIERADSVTLDPHKGMFLPYGTGCLLVREGRRLLD
                     AHAAGEARYLQDLAPEGESLNFSDYSPELSRDFRGLRVWLPLKLHGWEAFREALDEKL
                     DLARLLHEGIRDAPGIQVPWQPELSIVAFRSIGGEEASRALLERINGSQRIFLSSTMI
                     KGRFILRAAVLSFRTHRDRIEEAVEIIRSAAG"
     gene            complement(592..1320)
                     /locus_tag="VF097_00310"
     CDS             complement(592..1320)
                     /locus_tag="VF097_00310"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX6261268.1"
                     /translation="MEPLVAIGGRRLRPGSVGKWPDRGAAAVPDLFVQALHRAGAIEA
                     ILMPVEIDVDRAHAILDRFDALVLSGGGDIHPSRYGAEDHPKLWGVQAERDAFDLALA
                     QAAVERAMPFLAVCRGMQALNVALGGTLDPHLPEREGMIAHRKPGPDDDVLHDVRLEP
                     GTRTALAAGAQLVTGASSHHQGVEKLGKGLVATGWSEDGLVEAVEHEEGWAVGIQWHP
                     EVTAGYDPANQGLFDALVRRAARS"
     gene            complement(1330..2289)
                     /locus_tag="VF097_00315"
     CDS             complement(1330..2289)
                     /locus_tag="VF097_00315"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX6261269.1"
                     /translation="MTPPPVLVTRPLAEEVLGFLRERCEVEVPGEDRAMPRDELLDRL
                     SGKHGVLAMLTDRIDDEALDAAGPSLRVVANHAVGYDNVDVEACTRRGVIVTNTPDVL
                     TDATADMTWGLILSACRRIAEGDRLVRTGTPWEWSPGFMQGREVTGKTLGIVGFGRIG
                     RAVARRAAGFEMRVLVHTRTPPGDAQVVGLDELLTESDVVSIHLPLNEETRHRIGSRE
                     LGLMKETGVLVNTSRGSVVDEAALADALRTGRIAAAGLDVYEEEPSVHPRLLELENVV
                     LAPHLGSATVETRAAMAMLAARNLTAVLSGQPPLTPINPGALG"
     gene            complement(2286..2960)
                     /locus_tag="VF097_00320"
     CDS             complement(2286..2960)
                     /locus_tag="VF097_00320"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX6261270.1"
                     /translation="MTREHRRPAPRMRDLPAPRKNPSLAGPLFRAVWAWPYRLALAGL
                     HRMGVRPWQLTLLSLINNAICGWLIVSGRHFLGGILLLPAGLFDVFDGGVARLRGEES
                     RKGALLDAVNDRLADGIVFGAILYSLVDQGLMGAGKLALAALIVSLFVSHVRAEGEAR
                     GVDMSGGLFQRLERYIALMFGLTAPGMLAPVLAVLTGFGAFTILQRGVHAWRRLPRVG
                     AERQAP"
     gene            3026..4441
                     /gene="zwf"
                     /locus_tag="VF097_00325"
     CDS             3026..4441
                     /gene="zwf"
                     /locus_tag="VF097_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX6261271.1"
                     /translation="MALPPPDPVEIVIFGASGDLTRRKLLPALFHLFIGDLMPEDFGV
                     VGYARTEMSDEAFREMALEAVREHARCDVLGDTWSAFARRLHYVTGEFTEQGAFARLR
                     ERLDHLDREHGTDGRRLYYCGTPPVAYADIVRRLGEEGMAPDSRIVVEKPFGRDLETA
                     RELNRILHESFDEDQVYRIDHYLGKETVQNILAFRFANSIIEPLWNRRYVDHVQLTVA
                     EDEGIEGRGAFYEATGAMRDMVQAHLFQVLTFLAMEPPSGFEPEPLRDEKMKVLRSMR
                     PVDPSAVVRGQYEGYREEEGVDPASEMETYAALRVEIDNWRWAGVPFFLRTGKRMAEK
                     RAEATIQFKEPPHLVFERAGIDVDPNRLTLTIQPDEGISLSFMAQGPGVGMSLVPAAL
                     EFDYASAFDTELVEAYEVLLLEAMRSDRTLFLRADIVERAWDLLEPVLETPSQVVPYE
                     AGSWGPKDADDLIAPRNWFLT"
     gene            complement(4438..>4732)
                     /locus_tag="VF097_00330"
     CDS             complement(4438..>4732)
                     /locus_tag="VF097_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261272.1"
                     /translation="ILLRYAAHATQLARWDEATSSWTNLEATLLRGTLQIYGPSTELG
                     TFVALGPPTPPPPQDEGWPFWARWALYAGAGVVVVLAVEVYLRWRRRRTGNTG"
BASE COUNT          681 a         1702 c         1690 g          659 t
ORIGIN      
        1 acggagcggg gccgcgagcg cttccggggg atcgagcggg ccgactcggt gacgctcgac
       61 ccgcacaagg ggatgttcct cccgtacggc acgggctgcc tgctggtccg cgagggccgc
      121 cggctgctcg acgcccacgc cgcgggcgag gcccgctacc tccaggatct ggccccggag
      181 ggcgagtcgc tgaacttctc ggactactcc cccgagctct cgcgggactt ccgcgggctg
      241 cgggtgtggc tgccgctgaa gctccacggc tgggaggcgt tccgcgaggc cctggacgag
      301 aagctggacc tggcccgcct gctccacgag gggatccggg acgccccggg gatccaggtt
      361 ccctggcagc ccgagctctc gatcgtcgcg ttccgttcca tcggtggtga ggaggcgagc
      421 agggcgctgc tggagcgcat caacgggtcc cagcggatct tcctctccag cacgatgatc
      481 aagggccgct tcatcctgcg ggcggcggtc ctctcgttcc ggacccaccg ggaccggatc
      541 gaggaggcgg tcgagatcat ccgctcggcc gccggctgag gccgtccgcc gtcagctccg
      601 ggccgcccgg cgcaccagcg cgtcgaagag gccctggttg gcggggtcgt agcctgcggt
      661 cacctcgggg tgccactgga tgccgaccgc ccacccctcc tcgtgctcca cggcctccac
      721 gagcccgtcc tcgctccagc cggtggccac gagccccttg ccgagcttct ccaccccctg
      781 gtggtgcgag gatgcgccgg tcacgagctg ggcgccggcc gcgagcgccg tccgcgttcc
      841 cggctcgagc cggacgtcgt gcagcacgtc gtcgtccggc cccggcttac ggtgggcgat
      901 catgccctcc cgctcgggga ggtggggatc gagcgtgcct ccgagcgcga cgttcagcgc
      961 ctgcatacct cgacagacgg cgaggaacgg catcgcccgc tccacggccg cctgggccag
     1021 cgcgaggtcg aaggcgtcac gctccgcctg gacgccccac agcttgggat ggtcctcggc
     1081 cccgtacctc gacggatgga tgtcgccgcc tccgctcagc accagcgcgt cgaagcgatc
     1141 gaggatggcg tgcgcccgat cgacgtcgat ctccacgggc atgaggatcg cctcgatggc
     1201 ccccgctcga tggagtgcct ggacgaagag gtcgggcacc gccgccgcgc cgcgatcggg
     1261 ccacttgccg accgagcccg ggcgcagccg gcgccctccg atcgcgacga gcggttccat
     1321 gtccccagcc tagccgagtg cccctgggtt gatcggcgtg agaggcggct ggcccgagag
     1381 cacggccgtg aggttgcggg cggccagcat cgccatcgcg gcccgggtct cgaccgtggc
     1441 gcttcccaga tgcggggcga gcacgacgtt ctccagctcg agcagccgcg ggtggacgct
     1501 gggttcctcc tcgtagacgt cgaggccggc ggccgcgatc cgcccggtcc ggagcgcgtc
     1561 cgcgagcgcg gcctcgtcca cgaccgaacc ccgggacgtg ttcacgagca ctcccgtctc
     1621 cttcatcagg ccgagctcac gggacccgat gcggtggcgg gtttcctcgt tcagtgggag
     1681 gtggatcgag acgacgtcgc tctcggtcag cagctcgtcg aggccgacga cctgcgcgtc
     1741 gccgggcggc gtccgcgtat gcaccagcac gcgcatctcg aagccggccg ccctgcgggc
     1801 caccgcgcgg ccgatccgac cgaacccaac gatccccagc gtcttacccg tgacctcgcg
     1861 gccctgcatg aagcccgggg accactccca gggcgtcccg gtccgcacga ggcggtcgcc
     1921 ctccgcgatg cggcggcagg ccgagaggat cagcccccag gtcatgtcgg ccgtggcatc
     1981 ggtcaggacg tcgggggtgt tcgtcacgat caccccgcgc cgcgtgcagg cctcgacgtc
     2041 cacgttgtcg tagccgacag cgtggttggc cacgacgcgc agcgagggcc ccgccgcgtc
     2101 gagggcctcg tcgtcgatcc ggtccgtgag catcgccagc acgccgtgct tcccggaaag
     2161 ccggtccagc agctcgtccc ggggcatggc ccggtcctcg cccgggacct cgacctcaca
     2221 gcgctcccgc aggaagccga ggacctcctc ggccaggggt cgcgtcacga ggaccggcgg
     2281 aggggtcacg gagcctgccg ctccgctccc acccgcggaa ggcgccgcca agcgtggacc
     2341 ccccgctgaa ggatcgtgaa ggcgccgaac ccggtgagca ccgccaggac cggggccagc
     2401 atcccggggg cggtgaggcc gaacatgagc gcgatgtacc gctccagccg ctggaacagc
     2461 ccgccggaca tgtccacccc gcgagcctct ccttcggccc gcacgtggct cacgaagagg
     2521 gagacgatga gtgcggcgag cgcgagcttc cccgccccca tcagcccctg gtccacgagc
     2581 gagtagagga tcgccccgaa gacgatcccg tcggccagcc ggtcgttgac ggcgtcgagg
     2641 agggcgccct tcctcgactc ctctccgcgc agtcgggcca ccccgccgtc gaagacgtcg
     2701 aagagtccgg ccgggagcag caggatcccc cccaggaagt gccggccgga gacgatcagc
     2761 cacccgcaga tcgcgttgtt gatcagcgag agcagcgtga gctgccaggg acggacgccc
     2821 atccggtgga gcccggccag ggcgagccgg tagggccagg cccagaccgc ccggaagagg
     2881 ggcccggcca gactggggtt cttcctgggc gccggaaggt cgcgcatccg cggcgccggc
     2941 cggcgatgct ctcgagtcat cggcggcagt atccgccgcc ggccggcgcc gcggcaacga
     3001 gcggcggctg acataggatg acgagatggc gctgccgcct ccggatccgg tcgagatcgt
     3061 catcttcggg gcctccggtg acctcacccg ccgcaagctg ctgcccgccc tcttccacct
     3121 cttcatcggg gatctcatgc cggaggactt cggcgtcgtg ggctacgcgc gaaccgagat
     3181 gagcgacgag gcgttccgag agatggcgct ggaggccgtc cgcgagcacg cgcggtgcga
     3241 cgtcctcggc gacacctgga gcgcgttcgc ccggcgcctc cactacgtga cgggggagtt
     3301 cactgagcag ggcgccttcg cccggctgcg cgagcggctc gaccacctcg atcgggagca
     3361 cggaaccgat ggccgccgcc tgtactactg cgggacgccg ccggtggcct acgccgacat
     3421 cgtccggagg ctgggggagg agggcatggc gccggactcc cgcatcgtgg tggagaagcc
     3481 gttcggccgc gacctggaga ccgcccggga gctcaaccgg atcctccacg agtccttcga
     3541 cgaggaccag gtctaccgga tcgaccatta cctggggaag gagaccgtcc agaacatcct
     3601 cgccttccgg ttcgccaact cgatcatcga gccgctctgg aaccggcggt acgtggacca
     3661 cgtgcagctc accgtggccg aggacgaggg catcgagggc cggggcgcct tctacgaggc
     3721 caccggcgcg atgcgggaca tggtgcaggc ccacctcttc caggtgctga ccttcctggc
     3781 gatggagccc ccgtcgggct tcgagccgga gcccctgcgc gacgagaaga tgaaggtcct
     3841 gcgctcgatg cggccggtgg atccgtcggc cgtggtccgc ggccagtacg agggctaccg
     3901 cgaggaggag ggggtggacc cggcctccga gatggagacg tacgccgccc tccgcgtcga
     3961 gatcgacaac tggcggtggg cgggcgtgcc gttcttcctg cggacgggga agcggatggc
     4021 cgagaagcgg gccgaggcca cgatccagtt caaggagccc ccgcacctcg tgttcgagcg
     4081 cgcggggatc gacgtcgatc ctaaccgcct cacgctgacg atccagccgg acgaggggat
     4141 ctcgctgtcg ttcatggcgc aggggccggg ggttgggatg tcactcgtac ccgccgcgct
     4201 ggagttcgac tacgcctcgg ccttcgacac cgagctcgtg gaggcctacg aggtgctgct
     4261 cctcgaggcg atgaggagcg atcggacgct cttcctccga gccgacatcg tggagcgggc
     4321 gtgggacctg ctggagccgg tgctggagac gccctcgcag gtcgtcccct acgaggcggg
     4381 ctcgtggggg ccgaaggacg cggacgacct gatcgcgccg cggaactggt tcctgaccta
     4441 gccggtgttc ccggtccgcc ggcgccgcca ccgcaggtag acctccaccg ccaggacgac
     4501 cacgacgccg gcccccgcgt acagtgccca gcgggcccag aagggccacc cctcgtcctg
     4561 cggcggcggc ggcgtgggcg ggcccaaggc cacgaacgtc ccgagctccg tagaaggccc
     4621 gtagatctgg agggttcctc gcagcagggt ggcctccagg ttggtccacg agctcgtggc
     4681 ttcgtcccac cgcgccagct gggtggcgtg ggccgcgtag cggagcagga tc
//
LOCUS       DASUTZ010000009         5170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_908646, whole genome shotgun sequence.
ACCESSION   DASUTZ010000009 DASUTZ010000000
VERSION     DASUTZ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5170
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908646"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..134)
                     /gene="rnpB"
                     /locus_tag="VF097_00335"
     ncRNA           complement(<1..134)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VF097_00335"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(152..640)
                     /locus_tag="VF097_00340"
     CDS             complement(152..640)
                     /locus_tag="VF097_00340"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEX6261273.1"
                     /translation="MRLLSADPHPHLILHTDGAARGNPGPAGIGAVLTDPSGRTVATL
                     AQGVGVATNNQAEYRAVIEGLRLATERGAKRVLVRSDSKLLVEQLAGRFRVKNPTLQR
                     LHEEVRALVRTFDRVDFEHVRREENAEADALANEGVDAWLAGPGSGYEAADPRPPELF
                     EE"
     gene            complement(604..1305)
                     /locus_tag="VF097_00345"
     CDS             complement(604..1305)
                     /locus_tag="VF097_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEX6261274.1"
                     /translation="MDRILELQELDLSVDRLQARREEIESGKELAEARRRQEVLEGRL
                     GETRMALDGVTGEQARLENDVSMLTQKAEAEERRLYDGSVANPKELESIQAELKNLRD
                     RRSRLEDGILEQMERREQLEGGLPQLEAEVEEARARVAEIEVSSETELQDIGSALGER
                     AGERAALVHEIDEELLDLYEDLRSKKKGVGAAALVDGVCQGCRQQLSPLELDRLKKLD
                     GVRRCDYCRRILILT"
     gene            complement(1311..2423)
                     /locus_tag="VF097_00350"
     CDS             complement(1311..2423)
                     /locus_tag="VF097_00350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEX6261275.1"
                     /translation="MSGSPTAGGWIAALDELFPPAWAEAWDNVGLQVGDRAWPARRAL
                     VALDPTEEVVAEAVEWGCGLVVTHHPLLFRPRSSLDLRDPVARVAAAAIRAEIAIVAC
                     HTNADVAMPGVSDALAEALGIEVTGVLQGTGAGGRAKLVTFVPPESTAKVLDAVAAVG
                     AGVIGEYTHCSFRVTGTGTFLPTEAADPLIGERGELNQAEEDRVEIVVPRERVGSVVE
                     ALLQAHPYDEVAYDVYPLTGPTGLGLGRVGRLPDPITAGDLALACERELGARPRMAGD
                     PRRPVELVAVCGGSGASLIPEALAARVHAFVTGDVKHHAAQDAAAAGLVVIDAGHHAT
                     EWPFVPRLAERLSEAAASLGGDVIVSDLDTDPFRAP"
     gene            2520..3134
                     /locus_tag="VF097_00355"
     CDS             2520..3134
                     /locus_tag="VF097_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX6261276.1"
                     /translation="MRGWMRAAIAVGIVAGAVPITVAGATAAASCKGQPATIMGTSGH
                     DTIVGTPGNDVIVARGGDDLVRGRGGRDRICGGGGVDTLHGGPGRDFLFGGAGNDLLK
                     GGRSKDLVNGGPGSDFGYGGAGPDDMFGLGGRDVLRGQGGADFIFGDFGNDELYGNDG
                     DDCITTADGTSGNDLGKGGSGFDTSTSDPGDTLIGFEQEAVCGD"
     gene            complement(3131..3886)
                     /locus_tag="VF097_00360"
     CDS             complement(3131..3886)
                     /locus_tag="VF097_00360"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEX6261277.1"
                     /translation="MSTRTIALHRDGFPEPEWMDTAVSHAILRRASDGEATETLRLHR
                     PARLVAFGPRDRVAPGFPEAVEAARARGFGVVERLAGGRAAVFHEGTIAFSWVIPDEA
                     PRERIRRRFDAITEIVVEAFRALGVDARVGEVPGEYCPGEDSVNARGRVKLMGVGQRV
                     IARAAHIGGVIVVERSDLVRDALLPVYRALGIDWDPATAGSLAEEAPGVSWEDAEGAL
                     LDAFRERYGVEERPLPSETVELARDLAARHIPA"
     gene            3972..4724
                     /locus_tag="VF097_00365"
     CDS             3972..4724
                     /locus_tag="VF097_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261278.1"
                     /translation="MEPTVMTRPLPEAPAPPPRPAGRRPTIVILAIALALALVALLLG
                     GWAYLRRGDEIRSLRARLDRIERVGTARGAALGELRGNLRAARARIDRLEDRLADEIG
                     RRQAAESSLVGVAGPPLPDGRHFGYVRYLDLGGEIPRMLFDAAEWYVGDEADREAIED
                     GVIGPGERVENDIYIRNENALLRSIEVAGDARVLVVFLGRDPSQPTEIGLQRFARLIQ
                     DPSSRFAYARFLPFWATIEGGAVVEIEEQYVP"
     gene            complement(4729..>5170)
                     /locus_tag="VF097_00370"
     CDS             complement(4729..>5170)
                     /locus_tag="VF097_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261279.1"
                     /translation="WQVLLLAIPVLSFVRLLILARLARMGRLVSSALRGTRSARSRLG
                     SRLGWLVAVHLVVVLGVSEIAFQYLPFASFGEALYAVAMTSVSGQTLGIDRGLSQVLD
                     VLLATYAVVVFGALAGVLGSFFLERSRDEQEQGPPGESEGALTA"
BASE COUNT          704 a         1935 c         1843 g          688 t
ORIGIN      
        1 ttacctcccg agggaggcgg tctgctctct gtggcacttt ccgcgggtcg ccccgcctgg
       61 gtgttaccca gcaccctgcc ctgtggagtc cggactttcc tcaccccgaa gggcgcggcc
      121 gcccggcccg ctcctcgtac caaccttcag tctactcttc gaacagctcc ggcggccggg
      181 ggtcggccgc ttcgtagccg ctccccggac cggccagcca cgcgtccacg ccctcgttgg
      241 ccagggcgtc ggcctcggcg ttctcctccc ggcgcacgtg ctcgaagtcc acccggtcga
      301 aggtccggac gagcgcccgc acctcttcgt ggagccgctg aagggtggga ttcttcaccc
      361 ggaagcggcc cgcgagctgc tcgacgagga gcttgctgtc cgagcgcacg agcacgcgct
      421 tcgccccgcg ctcggtggcg agacgcaggc cctcgatgac ggcgcggtac tcggcctggt
      481 tgttcgtggc cacgccgacc ccctgggcca gcgtggccac cgtgcggccc gaggggtccg
      541 tgaggacggc gccgatgccc gcggggcctg ggttgccgcg ggccgcgccg tcggtgtgga
      601 ggatcaggtg aggatgagga tccgccgaca gtagtcgcac ctccggacgc cgtcgagctt
      661 cttcaggcga tccagctcga gcggcgagag ctgctggcgg cagccctggc acacgccgtc
      721 cacgagggcc gccgccccca cgcccttctt cttcgaccgg aggtcctcgt acaggtcgag
      781 cagctcctcg tcgatctcat ggacgagggc ggcccgctcg ccggcccgct cacccagcgc
      841 cgagccgatg tcctgcagct cggtctcgga ggagacctcg atctccgcga cgcgggcccg
      901 ggcctcctcg acctcggcct cgagctgggg caggcctccc tccagctgtt cccgccgctc
      961 catctgctcc aggatcccgt cctcgaggcg ggaccgccgg tcgcgcaggt tcttcagctc
     1021 ggcctggatc gactcgagct ccttggggtt ggccacgctg ccgtcgtaca gacgccgctc
     1081 ctcggcctcc gccttctggg tcagcatcga gacgtcgttc tccagccgcg cctgctcgcc
     1141 cgtgacgccg tccaaagcca tgcgggtctc ccccaggcgc ccctcgagca cctcttggcg
     1201 tcttcgcgcc tcggccagct ccttccccga ctcgatctcc tcgcggcgag cctggaggcg
     1261 gtccaccgac aggtcgagct cctggagctc caggatgcgg tccatccccc tcacggcgct
     1321 cggaaggggt cggtgtccag gtcggagacg atcacgtcgc ccccgaggct agcggcggcc
     1381 tcggacaggc gctcggccag gcgcggcacg aagggccact cggtggcgtg gtggccggcg
     1441 tcgatcacga cgaggccggc cgcggcggcg tcctgggcgg cgtggtgctt cacgtccccg
     1501 gtcacgaaag cgtggacgcg ggccgccagc gcctcgggga tcagcgaggc gccggagccg
     1561 ccgcagaccg cgacgagctc caccgggcga cgcgggtccc ctgccatccg ggggcgggcc
     1621 ccgagctcgc gctcgcaggc gagggcgagg tcgcccgcgg tgatcgggtc ggggagacgg
     1681 ccgacgcgac cgagcccgag cccggtcggg ccggtcagcg ggtagacgtc gtaggcgacc
     1741 tcgtcgtagg gatgagcctg gagcagggcc tcgaccacgg agccgacccg ttcccgaggg
     1801 accacgatct cgacgcgatc ctcctccgcc tggttcagct cgccccgctc cccgatcagc
     1861 gggtcggccg cctcggtggg taggaaggtg ccggtgccgg tcacgcggaa ggagcagtgc
     1921 gtgtactcgc cgatgactcc ggcgccgacg gccgcgaccg cgtccaggac cttggcagtc
     1981 gactccggcg ggacgaacgt cacgagcttg gcgcggccgc cggcgccggt cccctgaagg
     2041 acgccggtga cctcgatccc gagcgcctcg gccagcgcgt cggacacccc cggcatcgcc
     2101 acgtcggcgt tcgtgtggca cgccacgatc gcgatctcgg ctcggatcgc cgcggccgcc
     2161 acgcgagcga ccggatcccg gaggtcgagg ctcgaccggg gcctgaagag cagcgggtgg
     2221 tgcgtcacga cgagcccgca tccccactcc accgcctcgg ccacgacctc ctcggtcggg
     2281 tccagcgcca cgagcgcgcg tcgcgccggc caggcccgat cccccacctg gagcccaacg
     2341 ttgtcccagg cctccgccca ggcgggggga aacagctcgt cgagggcggc gatccatcct
     2401 ccggcggtcg ggcttcctga catgcgagcc gtagcatagc gcccgccgcc cgggccggac
     2461 cttcccgtgt cgggacgacg ggtgtacgct tcgccgcgct cggacgagag gagggcggga
     2521 tgcgcggatg gatgcgggcg gcgatcgccg tcgggatcgt ggccggggcg gtgcccatca
     2581 cggtcgcggg cgccacggcc gcggccagct gcaagggcca gccggcaacg atcatgggga
     2641 cgtcgggcca cgacacgatc gtcggcacgc ccgggaacga cgtgatcgtg gccaggggcg
     2701 gggacgacct ggtgcgcggc cgcggcgggc gggaccggat ctgcggcggc gggggcgtgg
     2761 acacgctcca cggcggcccg gggcgggact tcctcttcgg gggcgccggg aacgacctgc
     2821 tgaagggcgg gaggagcaag gacctcgtga acggaggtcc ggggagcgac ttcgggtacg
     2881 gcggcgcagg cccggatgac atgttcggcc tcggaggccg cgacgtgctc cgcgggcagg
     2941 gcggcgccga tttcatcttc ggcgacttcg ggaacgacga gctctacggg aatgacggcg
     3001 acgactgcat cacgaccgcc gacggcacga gcggcaacga cctggggaag ggagggtccg
     3061 ggttcgacac ctcgacgagc gatcccggcg acaccctgat cgggttcgag caggaggccg
     3121 tctgcgggga ctaggcgggg atgtggcgcg cggcgaggtc gcgggccagc tcgaccgtct
     3181 cggaggggag ggggcgctcc tcgacgccgt accgctcgcg gaaggcgtcg aggagcgccc
     3241 cctccgcgtc ctcccacgac acccccggcg cctcctcggc caggctgccc gccgtcgccg
     3301 gatcccagtc gatccccagg gcccggtaaa cgggcaggag cgcgtcccgc accaggtcgc
     3361 tccgctccac gacgatcacg ccgccgatgt gcgcggcacg ggcgatcacc cgctgcccca
     3421 cccccatcag cttcacccgc ccccgggcgt tgacgctgtc ctcccccggg cagtactcgc
     3481 ccggcacctc ccccacgcgc gcgtccacgc cgagcgcccg gaaggcctcg accacgatct
     3541 cggtgatcgc gtcgaaccgt cgacggatcc gctcccgcgg cgcctcgtcc gggatcaccc
     3601 acgagaaggc gatcgtcccc tcgtggaaga cggcggcccg acctcccgcg agccgctcca
     3661 cgacaccgaa gcctcgagcc ctggcggcct ccaccgcctc ggggaacccc ggcgccacgc
     3721 ggtcgcgggg accgaacgcg accagcctgg ccggccggtg caggcgaaga gtctcggtgg
     3781 cctcgccgtc cgacgcccgt cgcaggatcg cgtgcgagac cgccgtgtcc atccactcgg
     3841 gctcggggaa gccgtcccgg tgcagcgcga tcgtcctggt gctcatggtc gccaagcatc
     3901 ccacgtcccg cgggccgttc ggctctaggg ctcggcgccc cggcctccta cggtgtgatc
     3961 ggaggtgtgt gatggaaccc acggtgatga cccgacccct gcccgaggcc ccggctccgc
     4021 cgccgcgtcc cgccggacgc cgcccgacga tcgtcatcct ggcgatcgcg ctcgcgctgg
     4081 cgctcgtcgc gctcctcctg ggcgggtggg cctacctacg tcggggagac gagatccgct
     4141 ccctccgcgc gcgcctcgat cggatcgagc gcgtcggcac ggctcgcggc gccgcgctcg
     4201 gggagcttcg ggggaacctg cgcgccgccc gcgctcgcat cgaccgtctc gaggaccgcc
     4261 tcgccgatga gatcggccgg cgacaggccg ccgaatcctc gctcgtgggc gtggccgggc
     4321 cgccgctccc cgacggcagg cacttcggct acgtccggta cctcgacctg ggcggggaga
     4381 tcccgaggat gctcttcgac gccgcggagt ggtacgtcgg cgacgaggcc gaccgcgagg
     4441 cgatcgagga cggcgtcatc gggccgggcg agcgggtcga gaacgacatc tacatccgga
     4501 acgagaacgc cctcctccga tccatcgagg tggcgggcga cgcccgagtg ctcgtcgtct
     4561 tcctcggcag ggacccctcc cagccgaccg agatcggcct gcagcgcttc gccagactga
     4621 tccaggatcc ctcgtcccga ttcgcgtacg cgcggttcct ccctttctgg gcgacgatcg
     4681 aggggggcgc ggtggtcgag atcgaggagc agtacgtccc gtagaacgtc aggcggtgag
     4741 cgcgccctcc gactcccccg gagggccctg ctcctgctcg tccctgctgc gctccaggaa
     4801 gaagctgccc aagaccccgg cgagcgcccc gaacaccacg acggcgtagg ttgcgagcag
     4861 gacgtcgagg acctgggaca gcccccggtc gatgccgagc gtctggcccg acacgctcgt
     4921 catcgccacc gcgtagaggg cctcaccgaa gctcgcgaag ggcaggtact ggaaggcgat
     4981 ctcggagacg ccgaggacca cgaccaggtg gacggccacg agccacccca gccggctccc
     5041 gagccgcgac cgggccgagc gcgtgccgcg gagagccgag gagacgagcc ggcccatcct
     5101 ggccagacga gcgaggatca gcagccggac gaagctcagc acggggatgg cgagcagcag
     5161 gacctgccac
//
LOCUS       DASUTZ010000010         2854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1815358, whole genome shotgun sequence.
ACCESSION   DASUTZ010000010 DASUTZ010000000
VERSION     DASUTZ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1815358"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..153
                     /locus_tag="VF097_00375"
     CDS             10..153
                     /locus_tag="VF097_00375"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX6261280.1"
                     /translation="MIRGPGFSGEVVDFIDFHVWPVFNVADSAIFLGAATLLLLGLRR
                     TEP"
     gene            150..1094
                     /locus_tag="VF097_00380"
     CDS             150..1094
                     /locus_tag="VF097_00380"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX6261281.1"
                     /translation="MTRVTVRAAPGRLDAVLAEAAGISRADAQRAIEAGGVLVDGETR
                     PKSYRLQGGERIEADLPEAGRLEPEPGGVPVRYEDDHLLVVAKPPGLVTHPTASRRTG
                     TLVNRLLGMGMTLSAGSHPDRPGIVHRLDAGTSGLMLVAKSDAAHEGLVELLAARAVE
                     REYVALVRGVAEHDHFAVEAPLERRGARVRVRRGGGAEAETRFSVLERLEGATLLSAR
                     PQTGRTHQIRVHLASIGRPILGDRAYGGGGDDAKALGLERPFLHARRLALDHPITGDR
                     LEVEEPLPEDLEAALRHARGDRGAGGGTRPPRATGGRS"
     gene            complement(1021..1437)
                     /locus_tag="VF097_00385"
     CDS             complement(1021..1437)
                     /locus_tag="VF097_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261282.1"
                     /translation="MPPRKTSLAARVRATAEPFLGEKERVLKAAEAMKGTRTTMAALL
                     GPIGYFLLLRPRHVALTNRRIVVAIPPTRGGGTPKLDLALDRTATEVVEHRPGRLWTR
                     IVLQDEEGRRVALNFSRPWHEEAEFLRRRLGRRERA"
     gene            complement(1461..2576)
                     /locus_tag="VF097_00390"
     CDS             complement(1461..2576)
                     /locus_tag="VF097_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX6261283.1"
                     /translation="MRLSYSSISTYERCPAQFRFQYQERLPTAPGPALSFGSSLHEAL
                     RRFHDRPVPVAPPVEELLEMLEDVWISEGFRTPEEEVPYREHGRQVLRQYHLDNAADY
                     RIPAALEHHFSIDVDGVTVSGVIDRMDRLPGGGYEIIDYKTNRRLPPKRIVEDDLQLS
                     IYALAAREVWGIEPERLTLYYLLPGQRMTTTRSPAQLDDLRRRVAAVAERIEAGRFEP
                     QENRLCDWCDFQPRCPVFRHRYEEDGPPKIKEIVDEWVRLKREDRERWRRLEELGATI
                     RAYAEEHGLKRLYGSEGAVSLVRRVEQAIDPARVRELLEPLGRYEEVLTVDPRALGDL
                     VERRQLPPDVEDALLASREAVRVTTALHLRELDRSRR"
BASE COUNT          379 a         1013 c         1065 g          397 t
ORIGIN      
        1 accgaccgcg tgatccgggg gccgggcttc tccggcgagg tcgtggactt catcgacttc
       61 cacgtctggc cggtgttcaa cgtggccgac agcgcgatct tcctgggagc ggccacgctg
      121 ctgctcctgg ggctgcgccg cacggagccg tgacacgggt cacggtccgg gcggcgccgg
      181 gccggctcga cgcggtgctg gccgaggcgg cggggatctc ccgggcggac gcccagcggg
      241 cgatcgaggc cgggggcgtc ctcgtggacg gggagacgag gccgaagtcg tatcgcctcc
      301 agggaggcga gcggatcgag gccgatctgc ccgaggccgg ccggctcgag ccggagccgg
      361 gcggggtgcc ggtccggtac gaggacgatc acctcctggt cgtggccaag ccgcccggtc
      421 tcgtcacgca cccgaccgcg tcgcgccgga ccggcaccct ggtcaaccgc ctcctgggga
      481 tggggatgac cctctccgcg ggcagccatc ccgaccgccc gggcatcgtg caccggctgg
      541 acgcggggac ctccgggctg atgctcgtgg ccaagagcga cgccgcgcac gagggcctgg
      601 tggagctcct cgcggcgcgc gccgtcgagc gcgagtacgt cgcgctcgtg cgcggcgtcg
      661 ccgagcacga ccacttcgcc gtcgaggctc cgctggagcg ccgcggcgcg agggtccgcg
      721 tccggcgggg cggcggcgcc gaggccgaga cccgcttctc ggtcctggag cgcctggagg
      781 gcgccacgct gctgtcggcc cgcccccaga cgggacggac ccaccagatc cgggtccacc
      841 tggcctcgat cggccgcccg atcctggggg accgggccta cggcggggga ggggacgacg
      901 cgaaggcgct cggcctcgag aggcccttcc tccacgcccg tcgtctggcc ctcgaccacc
      961 ccatcacggg cgaccgtctc gaggtggagg agccgctccc cgaggatctg gaggctgcgc
     1021 tcaggcacgc tcgcggcgac cgaggcgccg gcggaggaac tcggcctcct cgtgccacgg
     1081 gcgggagaag ttgagggcga cgcggcggcc ctcctcgtcc tggaggacga tgcgggtcca
     1141 gagcctcccg ggacgatgct cgaccacctc ggtggccgtg cggtccaggg ctaggtcgag
     1201 cttgggcgtc cccccgcccc gggtaggggg gatcgcgacg acgatccgtc ggttcgtgag
     1261 cgccacgtgg cgggggcgca ggaggaggaa gtacccgatc gggccgagca gcgccgccat
     1321 ggtcgtccgg gtccctttca tcgcctcggc tgctttcagc acgcgctcct tctccccgag
     1381 gaacggctcg gcggtcgcgc ggacccgcgc ggcgaggctg gtcttcctgg gaggcaccgg
     1441 gtcagcctag cggagggcgg tcaccgccgg ctgcggtcga gctcgcggag gtggagagcg
     1501 gtggtcaccc ggacggcctc ccgggaggcg agcagggcgt cctccacgtc gggcggcagc
     1561 tggcgccgct cgacgaggtc ccccagcgcc cgggggtcca ccgtcagcac ctcctcgtag
     1621 cggcccaggg gctcgagcag ctcgcgaacg cgggcgggat cgatcgcctg ctccacccgc
     1681 cggacgagcg agaccgcccc ctcgctcccg tagaggcgct tcaggccgtg ctcctcggcg
     1741 taggcccgga tcgtggcccc cagctcctcc aggcgacgcc accgctcgcg gtcctcgcgc
     1801 ttgagccgca cccactcgtc cacgatctcc ttgatcttcg gtgggccgtc ctcctcgtag
     1861 cggtggcgaa acaccgggca ccgcggctgg aagtcgcacc agtcgcacag tcggttctcc
     1921 tgtggctcga atcgccccgc ctcgatccgc tcggccaccg cggcgacccg ccggcggagg
     1981 tcgtcgagct gggccgggga gcgggtggtg gtcatccgct ggccggggag gaggtagtag
     2041 agcgtgagcc gctcgggctc gatgccccag acctcgcgcg cggccagcgc gtagatcgag
     2101 agctggaggt cgtcctccac gatgcgcttc ggggggagcc ggcggttcgt cttgtaatcg
     2161 atgatctcgt agcccccgcc ggggaggcgg tccatgcggt cgatcacgcc cgagaccgtg
     2221 acgccgtcca cgtcgatcga gaagtggtgc tcgagcgcag ccgggatccg gtagtcggcc
     2281 gcgttgtcca ggtggtactg gcgaagcacc tgccgcccgt gctcgcggta ggggacctcc
     2341 tcctccggcg tccggaagcc ctcggagatc cacacgtcct ccagcatctc cagcagctcc
     2401 tcgacggggg gcgccacggg gaccgggcga tcgtggaacc gtcggagcgc ctcgtgcagc
     2461 gagctcccga acgacagggc ggggccgggc gccgtgggca gccgctcctg gtactggaag
     2521 cggaactgcg cggggcaccg ttcgtaggtg gatatgctcg aataggacag gcgcatcgcc
     2581 gtctcctctt gaccggcctc gggccggagt atagatttgg gagcgcagga attacaagga
     2641 tgtaatcaat ccacaggtta tccacagcgc gggaggccgt ggccgaccct tcccccggag
     2701 ggacagtgag cagcagcttc gtccatctcc acacgcacac ggagttctcg ctcctggacg
     2761 ggttcgcgcg catcacgccg ggcagcagcg cgccctcgat gctcgaccgg gccgccgaga
     2821 tggggatgcc ggcgatcggc ctcaccgacc acgg
//
LOCUS       DASUTZ010000011         5728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_649874, whole genome shotgun sequence.
ACCESSION   DASUTZ010000011 DASUTZ010000000
VERSION     DASUTZ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5728
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649874"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..440
                     /locus_tag="VF097_00395"
     CDS             90..440
                     /locus_tag="VF097_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261284.1"
                     /translation="MISRILIAVLAFAFGAAMPLSALGGEREEAVVREEDLWEETAYS
                     LDDDDDTDDSASSFTSGINSNDKTNSRHTPVTRDRDRSRGDLTRDRTKDGPGKSKRDW
                     TKNKTNDRSRNDTR"
     gene            complement(453..1100)
                     /locus_tag="VF097_00400"
     CDS             complement(453..1100)
                     /locus_tag="VF097_00400"
                     /inference="COORDINATES: protein motif:HMM:NF025166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like fold-containing protein"
                     /protein_id="HEX6261285.1"
                     /translation="MHADREAVAELLAAITYGERLAVRRLEDSVGLAPEPPVQKEQRH
                     VAERERERLELLEARLREMDGLALEDRFAPFFDAFFEATVPTDWVEAQTFHYVGDALV
                     SDLAEALLPTLDPVTAEVVRRAVGERDEQDAFALDQVTAALRDDPAETERIASYARTV
                     VGEALTQTRRALDASEAVSSLLGEPEDQKRILLDLLDRHRRRLDRLGIEPVEPEP"
     gene            1158..1406
                     /locus_tag="VF097_00405"
     CDS             1158..1406
                     /locus_tag="VF097_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261286.1"
                     /translation="MAAVVDLRGWREEHEPLELRLERAVMRLDALIEGRQPSDLPPWV
                     VTEVLAIQGCISLEMLEDAADRTERLVRRLRKGGFTEG"
     gene            complement(1549..2295)
                     /locus_tag="VF097_00410"
     CDS             complement(1549..2295)
                     /locus_tag="VF097_00410"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX6261287.1"
                     /translation="MEAAFFDLDKTIISKSSSLALTRPLYRAGFVTRSQLLRGAYAQM
                     VYLLLGADEKRMDRAKEGMLALSKGWDREELEALIRDVLEDVVDPFIYQEALDLMALH
                     KALGRRVFIVSSSPEEIVRPLANRLGLTDVVATRAEIEDGRYTGELEFYCYREQKGEA
                     IREVAEREGIDLSRSYAYSDSATDLPMLEAVGNPIAVNPDRDLRRIAEERGWQIRDFR
                     RPPGLDDPSSEARAAPRRLRADCDPLGPPV"
     gene            2346..2849
                     /locus_tag="VF097_00415"
     CDS             2346..2849
                     /locus_tag="VF097_00415"
                     /inference="COORDINATES: protein motif:HMM:NF039927.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5946 family protein"
                     /protein_id="HEX6261288.1"
                     /translation="MRDQETTTCPGCGVELPQRAGATHAYFNSSAECWAVFGKVSGRI
                     LQDVAVAGRAHQLLVDAYAAQHPGGEHPDKSIVIHLVGLHLMLERGVPPVEVPPLYHR
                     IASREGPWPHLPPPQDLGSLTIVDVARAETPEALAEAILSWAQSVWTAWSDHHAGVAR
                     LPERWLS"
     gene            complement(2853..3248)
                     /locus_tag="VF097_00420"
     CDS             complement(2853..3248)
                     /locus_tag="VF097_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010988197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX6261289.1"
                     /translation="MSPVRDAVVAVIRREERVLMVRRGPDAPMAGCWAPPGGRIEPGE
                     TQEEAVSREVREELGLEVRPVEKVWECPTHDGGYLLHWWLAEASAGEIRPDPAEVSEA
                     RWILPEEFDDLEPTFEAHRPFFEEILPTL"
     gene            complement(3269..3730)
                     /locus_tag="VF097_00425"
     CDS             complement(3269..3730)
                     /locus_tag="VF097_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6261290.1"
                     /translation="MSRGKFADRWAARPQAGGARRLADLQDAIAAAPLFAEVPKRHLR
                     SLALLSRVSSYPLGTEIVRQGTEGDTLYVVLEGEARIVKGRRTIARMKPGDFFGELAI
                     FDGEPRAASVVAEGPVLCLKLEGPDFRKVLLREPRLGLKIMTAMARRLRES"
     gene            complement(3861..4130)
                     /locus_tag="VF097_00430"
     CDS             complement(3861..4130)
                     /locus_tag="VF097_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261291.1"
                     /translation="MRRVLTVVFAGALLLGPASPALADPHPSQGAASEGRNEAGFGGG
                     PHCHVLITNRTPFDSTPAFPSHTAHLASGVGHVFAADPNCDGLPG"
     gene            4298..4390
                     /locus_tag="VF097_00435"
     tRNA            4298..4390
                     /locus_tag="VF097_00435"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4333..4335,aa:Sec,seq:tca)
     gene            complement(4865..5284)
                     /locus_tag="VF097_00440"
     CDS             complement(4865..5284)
                     /locus_tag="VF097_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX6261292.1"
                     /translation="MARKTVLQMWVEANDRIELVTPKDLAEEIASGQILLLDVRLPIE
                     IERRGTIAGSVPVPRQVVEWWADPDSPYFRPDGVFGNFDRRVITFCDGGGNGAFTAVA
                     LQELGYRNVATLEGGFYGWVGAGLPVLGPSDRPPSDT"
     gene            complement(5453..5599)
                     /locus_tag="VF097_00445"
                     /pseudo
     CDS             complement(5453..5599)
                     /locus_tag="VF097_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034561.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
BASE COUNT          900 a         2050 c         1910 g          868 t
ORIGIN      
        1 agggtgagag gaccctcatc cccctccaac ggacgtctca tccggcgggc tcacactggg
       61 gccatgttcg gaggacgaag gaggagcaga tgatctcgag gatcctgatc gccgtcctgg
      121 cgttcgcgtt cggggccgcg atgccactct ccgcgctcgg cggggagcgc gaggaggccg
      181 tggtccggga ggaggatctc tgggaggaga ccgcctacag cctggacgac gacgacgaca
      241 ccgacgacag cgcgtccagc ttcaccagcg gcatcaacag caacgacaag acgaacagcc
      301 ggcacacgcc ggtgacccgc gaccgggacc gcagccgcgg cgacctgacc cgagaccgga
      361 cgaaggacgg ccccggcaag agcaagcggg actggacgaa gaacaagacg aacgaccgct
      421 cccggaacga cacccgctga gcgggcggcg ctctagggct ccggctcgac cggctcgatc
      481 ccgagccggt cgagccgtct gcggtgccga tccagcagat ccaggaggat ccgcttctgg
      541 tcctcgggct ccccgagcag cgacgagacc gcctcgctgg cgtcgagggc ccgtctggtc
      601 tgcgtgagcg cctcgccgac gacggtccgt gcgtaggagg cgatccgctc ggtctccgcc
      661 gggtcgtcgc gcagggccgc cgtcacctga tcgagagcga aggcgtcctg ctcgtctcgc
      721 tcccccaccg cgcgccggac gacctcggcc gtcacgggat cgagggtggg gaggagggcc
      781 tccgcgaggt ccgagacgag ggcgtcgccc acgtagtgga aggtctgcgc ctccacccag
      841 tcggtgggga ccgtggcctc gaagaacgcg tcgaagaagg gcgcgaagcg atcctcgagg
      901 gccaggccgt ccatctcccg caggcgggcc tcgagcagct ccagccgctc gcgctcgcgc
      961 tccgcgacgt gccgctgctc cttctgcacc ggcggctcgg gggcgagccc cacagaatcc
     1021 tccagccttc gcacggcgag gcgctccccg tacgtgatcg cggcgagcag ctcggcgacg
     1081 gcttcgcgat cggcatgcat gaggccattc taggcgggcc cgggttcgtg tcagtggcgg
     1141 tcgctaccgt gacggccatg gctgcggtgg tggatctccg aggatggcgc gaggagcacg
     1201 agcccctcga gctcaggctg gaacgggccg tgatgcgcct cgatgccctt atcgaggggc
     1261 gccagccgtc cgacctgccc ccctgggtgg tcaccgaggt gctggcgatc caggggtgca
     1321 tctcgctcga gatgctggag gacgccgccg accgcaccga gcggctggtg cggcgcctgc
     1381 ggaagggagg gttcaccgaa ggctgaccac agacgaagag ggcagctacc aggcgctctc
     1441 atcatcgacc cgggtcgccg ggaggcgggt ccgggccttg cctcccgcgg cagatcgagc
     1501 gtgggttctg ccctcttcgg aggaacctcg cggcccctcg aggccatcct ataccggggg
     1561 cccgaggggg tcgcaatcgg ccctcagccg ccgcggcgct gcgcgagcct cggacgaagg
     1621 atcgtccaga ccaggagggc gccggaagtc ccggatctgc cagcctcgct cctcggcgat
     1681 cctccgcaga tcgcgatcgg gattgaccgc gatcgggttg cccaccgcct ccaacatcgg
     1741 gaggtcggtc gccgagtccg agtacgcgta ggaccttgag aggtcgatcc cctctcgctc
     1801 ggccacctcg cggatcgctt cccccttctg ctcccggtag cagtagaact cgagctcccc
     1861 tgtatacctc ccgtcctcga tctccgcccg ggtcgcgacc acgtcggtga gccctaagcg
     1921 attcgccagc gggcgcacga tctcctccgg cgatgacgag acgatgaaca ccctccgccc
     1981 cagggccttg tggagggcca tgaggtcgag ggcctcctga tagatgaacg gatccaccac
     2041 gtcctccagc acgtcccgga tcagcgcctc gagctcctct cggtcccatc ccttggagag
     2101 ggccagcatc ccctccttgg cccggtccat ccgcttctcg tccgccccga gcagcagata
     2161 gaccatctgc gcataggcgc cccggaggag ctgcgacctg gtcacgaagc cggctcggta
     2221 gagcggcctg gtgagggcca gggagctcga cttcgagatg atcgtcttgt cgaggtcgaa
     2281 gaacgcggcc tccatgtagg ccatggtatc gcccctctcg ggatgctcgg cgctatgctc
     2341 cgccggtgcg cgaccaggag acgacgacat gccccgggtg cggtgtcgag ctaccccagc
     2401 gggcgggcgc cacccacgcc tacttcaact cctcggccga gtgctgggcg gtgttcggca
     2461 aggtgtccgg tcggatcctc caggacgtgg ccgtcgcggg ccgggcccat cagctcctcg
     2521 tggacgccta cgcggcccag caccccggag gcgagcatcc cgacaagtcc atcgtgatcc
     2581 acctcgtggg cctccacctg atgctggagc gaggggtccc gccggtcgag gtcccgcccc
     2641 tctaccatcg gatcgcgagc cgggagggtc cctggcccca cctccctccc ccccaggacc
     2701 tggggtcgct gacgatcgtg gacgtcgctc gggcggagac acccgaggcg ctggccgagg
     2761 cgatcctctc ctgggcacag agcgtgtgga cggcgtggtc ggatcaccat gccggggtgg
     2821 cccggctccc ggagcgctgg ctctcctgac gctcagagcg tcgggagaat ctcctcgaag
     2881 aacgggcgat gggcctcgaa ggtgggctcg agatcatcga actcctccgg gaggatccat
     2941 cgagcctcgc tcacctccgc cgggtcgggc cggatctccc cggcgctcgc ctccgccagc
     3001 caccagtgca ggaggtaccc gccgtcgtgg gtggggcact cccagacctt ctccaccggc
     3061 cggacctcga ggccgagctc ctcgcgcacc tcgcgcgaga ccgcctcctc ctgtgtctcc
     3121 cccggttcga tcctgccgcc cggcggcgcc cagcagccgg ccatcggagc gtcggggccc
     3181 cgccggacca tgaggacccg ctcctccctc cggatcacgg ccacgaccgc gtcccgcacg
     3241 ggggacatgc cggacagggt acccgggctc aggactctcg gagccgcctg gccatcgccg
     3301 tcatgatctt caggccgagc cgcggctccc gcagcagcac cttgcggaag tccggtccct
     3361 cgagcttcag gcagaggacc ggtccctcgg ccacgaccga ggccgcgcgg ggctccccgt
     3421 cgaagatggc cagctctccg aagaagtccc ccggcttcat gcgagcgatc gtgcgccgcc
     3481 ccttcacgat ccgggcctcg ccctccagga ccacgtacaa cgtgtctccc tcggtcccct
     3541 ggcggacgat ctcggtcccc agggggtacg acgacacccg gctgagcagc gcgagcgatc
     3601 ggagatggcg cttcgggacc tcggcgaaca gcggcgccgc ggcgatcgcg tcctggaggt
     3661 cggcgagcct tcgagcgccg cccgcctgag gcctggcggc ccaccgatcc gcgaacttcc
     3721 ccctggacat caggccgacc tcctcatggc tggctcacct tggagcaggc ctccgagggt
     3781 gtcaagcatg ccccgccccg gagacgcagg gggccggccg gtggggctcc cgctcgagag
     3841 cgggagcccc accgccccgg tcaacccggc agcccgtcgc agttcgggtc ggcggcgaac
     3901 acgtgcccga ccccggacgc aaggtgtgcc gtgtgggagg ggaaggcggg cgtgctgtcg
     3961 aacggggtgc ggttcgtgat gaggacgtgg cagtgcggcc ctcccccgaa accggcctcg
     4021 ttgcgcccct cgctcgccgc cccctgggac gggtgtggat cggcgagcgc cggacttgcc
     4081 ggtcccagga gaagcgcccc cgcgaagacc accgtgagta cccttcgcat cgaacctcct
     4141 ttcgctcgga cgacccgggt ggtcgccctc ggatggacct catccggcat tcgatgggtc
     4201 cgcggtgagg gttcgctcag ccggcccagg atcgtccgtg gctccggaaa gatccggagg
     4261 cacgcttcgc ctcacgccgc cgcgtatcct cggaggcgga ggcgtcaggg ttcctggtgg
     4321 gccccccggt cttcaaaacc ggtgaggagg cgacgagccg cctggcgggt tcgattcccg
     4381 tccgcctccg ctcttacatc gatgtaatcc ggggtcgccg gttgggcaca cgcctcacga
     4441 ccgcgtcctc cagaaaggag gcttcacctc gtggtgatcg aggccacgaa tggcccgcag
     4501 gcgcccgacc tgcccagccg ctcgcctatc ccggccgctt caccaatctc gcccgaagtt
     4561 gctggtagat ggacgctatg tcaaccacgc ccgaacccgg cgggatcggt gccggtcatc
     4621 gccgcaggcg gggagatcat ggtcggggag ccactgttcg tgaggcggac atcctggcgt
     4681 cgaacggcat cgtccacgcg gtggacgcgc tgatcgaggg agcaccggcc agttcctgat
     4741 cccgccttcg cgcgattacc ccctcggaca ccggcacaag cgtggcgaac gtcgtgcagg
     4801 tgtcgcggag ttcgggccct acaagatccg tcgatgtatc cggaacggcg cggggacacc
     4861 cagattaggt atcgctcggg ggtctgtcgg aggggccgag gacgggcagg cctgcaccga
     4921 cccacccgta gaagccgcct tcgagcgtgg ctacattgcg atagccgagt tcttgcagcg
     4981 ccacagcagt gaaagcaccg tttccgccgc catcgcagaa cgtgatcacc cttctgtcga
     5041 aattcccgaa gacgccgtcc ggccgaaaat acggactatc cggatcagcc caccactcca
     5101 ccacttgccg cggtacggga actgatccgg caatggtccc ccgccgctcg atctcgatgg
     5161 gtaaacgaac atccagaagc aggatctgac cggaggcgat ctcctcagcg aggtctttgg
     5221 gcgtcaccaa ctcgatccga tcgttggctt cgacccacat ctgcagcacc gtcttccgcg
     5281 ccaccactac atggtcacca ccaagtaccc gactacccga atgttagtac acggtcgcgg
     5341 cgactccgcg ggcggttccg ccagcctgga gcacgccacc cggatcgggt gcattgcgtt
     5401 gcggggcggt cctagacccg agaagcattt gcgtactcgg cccggcgaac ctctcaggag
     5461 gatccgatgg cttgccgcag ccgagcgcat cccaggccac caccgtgaat ccgtcggaca
     5521 gatcatcgag ctgccggcgc catacccggc tgtccgtaag cccgccgtgg aggagaacga
     5581 caggtgaccc ttcgcccatc cggtagaacg cgatgggcgt tcctcgaatt cgtcgggagc
     5641 ttcgccccca tgcgcgggga gcaaccggat cttctggctc ggtgtgctgg cgttcgcacg
     5701 ctgtagccgt cagcttccgc tcttatgg
//
LOCUS       DASUTZ010000012         6909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1686501, whole genome shotgun sequence.
ACCESSION   DASUTZ010000012 DASUTZ010000000
VERSION     DASUTZ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6909
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686501"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VF097_00450"
     CDS             <1..220
                     /locus_tag="VF097_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003014486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261293.1"
                     /translation="SLGTDGFGRSDTRPALRRFYEVDAEHVVVATLAALAEMGEVKPE
                     QVAEAIDRYEIDPERGAPFHPDAGTSLA"
     gene            342..677
                     /locus_tag="VF097_00455"
     CDS             342..677
                     /locus_tag="VF097_00455"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HEX6261294.1"
                     /translation="MLALAFNAVVLALIDGVVMPLIAAAVGEPNFDELTFTVGEGVIR
                     YGSFLTALVNFLVIAFVPFLIVKGDQRAARSGEEEAVVRGCPFCTTDIPVAATRCPSC
                     TSQVEPLAT"
     gene            728..2500
                     /gene="thrS"
                     /locus_tag="VF097_00460"
     CDS             728..2500
                     /gene="thrS"
                     /locus_tag="VF097_00460"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX6261295.1"
                     /translation="MADADRLEVLRHSTAHVMAQAVCDLFEGAKYAIGPPIEDGFYYD
                     FDLPETLTPEDLERIEARMREIVEEDQPFVREELSRDEALERFAGQPYKIEIIAGVEE
                     EEGAGAESVSVYRNDGWADLCRGPHVGSTAELGAFRLTSVAGAYWRGDENRPQLQRIY
                     GTAWETQEELETYLRRIEEAEQRDHRRLGRDLDLFSFPDELGAGLSVWHPKGGVIRNE
                     LERWVRELHVERGYELVATPHLARSVLWEMSGHLEKFAESMYPAMSADDGSDYYVKPM
                     NCPFHVLIFRSRTRSYRELPLRLSELGTVYRYEKPGVLHGLLRVRGLTQDDSHIICTP
                     DQLTEEILGVFDLTLEIHRTFGFEDPVIHLSTQPGQAIGDEEMWERATQALREALDRS
                     GMPYELAEGEGAFYGPKVDFHFHDAIGRLWQLTTVQVDFALPERFDMEYMGEDNQRHR
                     PVMIHRAILGSVERFLGVLIEHFAGAFPPWLSPVQARIVPIADRHAEHAEGLARRLRQ
                     GGLRAEVDASRETLGKKIRDAQLQKVPYALVVGDKEIEADTVSVRDRSGTEARGVSVE
                     AFVRRAAEEVRSRAVETGPVGDLA"
     gene            2500..2988
                     /locus_tag="VF097_00465"
     CDS             2500..2988
                     /locus_tag="VF097_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004067146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX6261296.1"
                     /translation="MERLWTPWRMEYIKSATTGDDAGCIFCELPQEGDDERTLILARR
                     EGAFVILNRFPYNPGHLMVAPFRHVGELTDLEDDELAETVSLVRDAVRALRDEWEPSG
                     FNVGMNLGRVAGAGVPGHLHWHVVPRWHGDSNFMPVIGQTKVLPELLQETYGKLRPAF
                     EG"
     gene            2991..4307
                     /locus_tag="VF097_00470"
     CDS             2991..4307
                     /locus_tag="VF097_00470"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6261297.1"
                     /translation="MPDAARRIVRTYLVISGLFYLAASIIWGVNTLFLLDAGLTLFEA
                     FVANAAWTAGMVVFEIPTGLVADTRGRRTSFLLSASILALGTIGYVVISWVGGGIVPF
                     VLVSIVLGLAYTFYSGAVEAWLVDALRENGYTGELDPVFAKGGVVASAAMLVGTVSGG
                     ALGMLDLGFPYLARAALLLLAFGVAFAWMRDEGFTPRALRLRDVRREVGDVARASVRH
                     GWGRPAVRLLMLVSFIQFGVGAWAFYAWQPYFLDLLGRGDLIWVAGVVAALIALAQIG
                     GNALVRIVAKPGRRRSTILLVGAAAYAVSLAVVGMVSSFYLAVAIFVGGILVSAMATP
                     IKQAYLHEMIPSAQRATVISFDGLFGSLGSVGGQVGLGWLSQTRSIPAGFVVSGVSAL
                     FALPLLALLRGRRERPDVIRSPAPDEEAAAEVAGAGGEAATDAAPR"
     gene            complement(4304..5266)
                     /locus_tag="VF097_00475"
     CDS             complement(4304..5266)
                     /locus_tag="VF097_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6261298.1"
                     /translation="MALDGFDLEASRGVLLALLGPSGCGKTTALRVIAGFDRPDAGTV
                     EIAGHRVAGAGTWVPPERRRVGMVFQDWALFPHLDVERNVAFGLPRDERHRVRELLEM
                     VHLDGMERRMPHELSGGQQQRVALARALAPSPEIILLDEPFSNLDATLRAGVRAEVRR
                     VLHEAGVTAIFVTHDREEALAIADEVAVMAEGRILQTGIPHEVYERPSDPLVAGLLGD
                     ANLIPADVRGGLIETPLGALPADGWRDGPVGLVVRPESIALEPDPSSEALVEDVEFYG
                     HDQLFGVRLPDGSLLKVRCFGARDDLRVGTPVRARLLAAPRLYR"
     gene            complement(5317..>6909)
                     /locus_tag="VF097_00480"
     CDS             complement(5317..>6909)
                     /locus_tag="VF097_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002609489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6261299.1"
                     /translation="AARARPPRPTRAPWGLLAAGLAVSALVVLPVAYLVIRVADADAA
                     SWDLILRSRTAWLALRTIGLAAAVTAAAVTLGVALAWLTTRTDLPFRRVWSVVLALPL
                     VFPSYVGAFVLISALGPRGMFQQLLSPLGVERLPDISGFPGAFLALTLFTYPYVFLMT
                     AAGIRGIGSSMEEASRSLGHSRWRTFRAVTLPLLRPSIAAGALLVALYTVQDFGAVSL
                     MRFPAFTQAIYLQYRGAFDRTPAAILSLMLVALALAILSLERRARGRARYYRAEGGAE
                     RPLSPIPLGRWRWPALVVCGIVAALGLVLPVSVLGFWMIRGLAGGGETTPILGPAGSS
                     VVASGAGAAIAVVAGLPVAMLAARYGGRWVSLTERTTYLGYALPGIVVALAFVFFAAN
                     YLPGLYQSLPLLVIVYVVLFLPQASSPLRGALLQVSPRIEEAGRALGHGPWTSFRRLV
                     LPLISRGALAGFALVFLTALKELPATLLLRPTGFETLATEVWTAASVGLYSRAALPAL
                     VLVAVSAVPLYVLTTRVEVRER"
BASE COUNT         1015 a         2362 c         2613 g          919 t
ORIGIN      
        1 ctcgctcggg accgacgggt tcggacgctc cgacaccagg ccggcgctgc gccgcttcta
       61 cgaggtggac gcggagcacg tcgtcgtggc cacgctcgcg gcgctggccg agatgggcga
      121 ggtgaagccc gagcaggtgg ccgaggcgat cgaccggtac gagatcgacc ccgagcgagg
      181 ggcgcccttc caccccgacg cgggcacctc cttggcctga cctggcaccg ggagcgcgga
      241 gagggcatcc tcgcgtcatc cgaccgagga ggtggcgatg ggcctctgga gggacttcaa
      301 ggggttcgcg ctcaagggca acctgctcga cgatcgcggt cgtgctcgcc ctagctttca
      361 acgcggtcgt gctggccctg atcgacgggg tcgtgatgcc gctgatcgcc gctgcggtgg
      421 gcgagcccaa cttcgacgag ctgaccttca ccgtcggaga gggcgtgatc cgctacgggt
      481 cgttcctcac cgcgctggtc aacttcctcg tgatcgcctt cgtgccgttc ctgatcgtca
      541 agggggacca gcgggccgcg cgatcgggcg aggaggaggc cgtcgtccgg gggtgcccgt
      601 tctgcacgac ggacatcccg gtcgcggcga cgcgctgtcc cagctgcacg agccaggtgg
      661 agccgctggc gacctgacgg cggctatcat ggcgtccgcg cgcccggcgc ggtgaaggag
      721 cagggggatg gcggacgctg atcggctcga ggtcctgcgg cactcgaccg cccacgtgat
      781 ggcccaggcg gtgtgcgacc tcttcgaggg ggcgaagtac gcgatcgggc ccccgatcga
      841 ggacggcttc tactacgact tcgacctccc cgagacgctc acgcccgagg acctggagcg
      901 gatcgaggcc cgcatgcgcg agatcgtgga ggaggaccag cccttcgtcc gggaggagct
      961 ctcgcgggac gaggccctgg agcggttcgc cggccagccc tacaagatcg agatcatcgc
     1021 gggcgtggag gaggaggagg gcgccggcgc cgagagcgtc tcggtctacc gcaacgacgg
     1081 ctgggccgac ctctgccggg ggccgcacgt cgggtccacg gccgagctcg gcgccttcag
     1141 gctgacgagc gtggccggcg cctactggcg gggcgacgag aaccgccccc agctccagcg
     1201 gatctacggc acggcctggg agacccaaga ggagctggag acctacctcc ggaggatcga
     1261 ggaggccgag cagcgcgacc accgacggct gggccgggac ctcgacctgt tctcgttccc
     1321 cgacgagctc ggggcgggcc tctccgtctg gcatccgaag ggcggcgtga tccggaacga
     1381 gctggagcgg tgggtgcgtg agctccacgt ggagcgcggc tacgagctcg tggccacccc
     1441 ccacctggcc cggtcggtgc tgtgggagat gagcggccac ctggagaagt tcgcggagag
     1501 catgtacccg gcgatgtcgg ccgacgacgg ctccgactac tacgtgaagc cgatgaactg
     1561 cccgttccac gtcctgatct tccggtcgcg cacgcgctcg taccgcgagc tgccgctgcg
     1621 gctctccgag ctggggaccg tgtaccggta cgagaagccg ggggtgctgc acgggctgct
     1681 ccgcgtgcgc gggctcaccc aggacgactc ccacatcatc tgcacgccgg accagctgac
     1741 cgaagagatc ctgggggtct tcgatctcac gctcgagatc caccggacgt tcgggttcga
     1801 ggacccggtg atccacctct cgacccagcc gggccaggcg atcggcgacg aggagatgtg
     1861 ggaacgggcc acccaggctc tgcgcgaggc gctggaccgg agcggcatgc cgtacgagct
     1921 cgcggagggc gagggcgcgt tctacgggcc gaaggtggac ttccacttcc acgacgcgat
     1981 cgggcgcctg tggcagctca cgaccgtgca ggtcgacttc gccctgcccg agcgcttcga
     2041 catggagtac atgggcgagg acaaccagcg gcaccgcccc gtgatgatcc accgggcgat
     2101 cctgggttcg gtggagcggt tcctgggggt gctgatcgag cacttcgcgg gggcgttccc
     2161 cccgtggctc tcgccggtgc aggcccgcat cgtcccgatc gccgaccgac acgcggagca
     2221 cgccgagggt ctcgcgcgac gcctccgcca gggcgggctg cgcgccgagg tcgacgcgtc
     2281 gcgcgagacc ctcggcaaga agatccggga cgcccagctc cagaaggtcc cctacgccct
     2341 tgtggtggga gacaaggaga tcgaggcgga caccgtctcg gttcgcgacc ggtcggggac
     2401 cgaggcccgc ggcgtctccg tggaggcgtt cgtgcggcgg gccgcggagg aggtccggtc
     2461 tagggccgtg gagaccgggc ccgtcgggga cctcgcctga tggagcgtct ctggacgccg
     2521 tggcgcatgg agtacatcaa gtcggcgacc acgggcgacg acgccggctg catcttctgc
     2581 gagctgccgc aggagggcga cgacgagcgg acgctgatcc tggcgaggcg cgagggggcg
     2641 ttcgtgatcc tcaaccggtt cccctacaac cccggccacc tcatggtggc gcccttccga
     2701 cacgtcggtg agctcaccga cctcgaggac gacgagctgg ccgagaccgt gtccctcgtc
     2761 cgggacgccg tccgagcgct ccgcgacgag tgggagccca gcggcttcaa cgtgggcatg
     2821 aacctggggc gggtggccgg ggccggcgtg cccgggcacc tccactggca cgtcgtgccc
     2881 cgctggcacg gcgactccaa cttcatgccg gtgatcggcc agacgaaggt tctgcccgag
     2941 ctcctccagg agacgtacgg gaagctgcga cccgccttcg agggctgacg gtgccggacg
     3001 ccgctcggag gatcgtccgg acctacctcg tgatcagcgg gctgttctac ctggctgcct
     3061 cgatcatctg gggggtcaac accctcttcc tgctcgacgc cggcctgacg ctgttcgagg
     3121 cgttcgtggc caacgcggcc tggaccgcgg ggatggtggt gttcgagatc ccgacgggcc
     3181 tcgtcgcaga cacgcggggg cgccggacgt cgttcctcct ctcggcctcg atcctcgctc
     3241 tgggcacgat cggctacgtc gtgatctcgt gggtcggcgg agggatcgtg ccgttcgtcc
     3301 tggtctcgat cgtgctcggc ctggcctaca ccttctactc gggcgccgtg gaggcctggc
     3361 tcgtggacgc gctccgcgag aacggataca cgggcgagct cgaccctgtc ttcgccaagg
     3421 ggggggtcgt ggccagcgcc gcgatgctcg tggggacggt gagcggcggc gcgctgggca
     3481 tgctcgacct ggggttcccg tacttggcgc gggcggccct cctgctgctg gcgttcgggg
     3541 tggcgttcgc ctggatgcgg gacgagggct tcacgccgag ggccctgcgt ctccgggacg
     3601 tgcgccggga ggtgggcgac gtcgctcgcg cgagcgtacg tcacgggtgg ggacggcccg
     3661 ccgtgcgcct cctcatgctc gtctcgttca tccaattcgg ggtgggggcc tgggcgttct
     3721 acgcctggca gccgtacttc ctggacctgc tcgggcgggg agacctgata tgggtggcgg
     3781 gggtggtggc cgcgctgatc gcgctggctc agatcggcgg gaacgcgctc gtgcggatcg
     3841 tggccaagcc aggccgccgc cgctccacga tcctcctcgt cggggcggcc gcctacgccg
     3901 tctcgctcgc cgtcgtgggg atggtgtcga gcttctacct cgcggtcgcg atcttcgtgg
     3961 gggggatcct cgtctcggcg atggccacgc cgatcaagca agcctatctc cacgagatga
     4021 ttccgtccgc ccagcgggcc accgtgatct cgttcgacgg gctcttcggg agcctgggga
     4081 gcgtcggggg ccaggtcggg ctgggctggc tgtcgcagac gcgctcgatc ccggcggggt
     4141 tcgtggtgtc gggcgtctcg gcgctgttcg ccctcccgct gctggccttg ctgcggggga
     4201 ggcgagagcg tcccgacgtg atccgcagcc cggcccccga cgaggaggcg gcggcggagg
     4261 tggcgggcgc gggaggcgag gcggcgacgg acgccgcgcc gcgttagcga tagaggcgcg
     4321 gggcggccag gaggcgggcc cgcaccggtg tgcccacccg gaggtcgtcg cgggccccga
     4381 agcagcggac cttgagcagg ctgccgtcgg ggagccgcac cccgaagagc tgatcgtggc
     4441 cgtagaactc cacgtcctcc acgagggcct cgctcgaggg gtcgggctcc agggcgatgc
     4501 tctcgggccg caccacgagg cccaccggcc cgtcccgcca gccgtcggcc ggcagagccc
     4561 cgagcggcgt ctcgatcagg ccgccccgga cgtccgcggg gatcaggttc gcgtcgccca
     4621 gcagcccggc cacgagcggg tccgagggcc gctcgtagac ctcgtggggg atgccggtct
     4681 ggaggatcct cccctccgcc atcaccgcga cctcgtcggc gatcgccagc gcctcctcgc
     4741 ggtcgtgggt gacgaagatc gccgtcacgc cggcctcgtg gagcacacgg cggacctcgg
     4801 cccggacccc ggcccggagc gtggcgtcca gattcgagaa gggctcgtcc agcaggatga
     4861 tctcgggcga gggggcgagg gcacgggcca gagccacgcg ctgctgctgg ccccccgaca
     4921 gctcgtgcgg catccgccgc tccatcccgt cgaggtggac catctcgagg agctcccgca
     4981 cccgatgccg ctcgtcgcgc ggcagcccga aggcgacgtt gcgctccacg tccaggtggg
     5041 ggaagagcgc ccagtcctgg aaaaccatgc ccacccggcg ccgctccggg ggcacccacg
     5101 tgccggcccc cgccacgcgg tggcccgcga tctcgaccgt ccccgcgtcg ggtcggtcga
     5161 agccggcgat cacccgtagg gcagtggtct tgccgcaccc gctcgggccc agcagggcga
     5221 ggaggacccc gcggctcgcc tcgaggtcga agccgtccag ggccacggtg tcgtcgaacc
     5281 gcttcgatac ccctcggacc cgtaggaccg tctcgctcag cgctctcgca cctccacccg
     5341 cgtcgtcagc acgtacagcg gcacggccga gaccgcgacc agcaccaggg cgggcagcgc
     5401 cgccctcgag tacagcccga ccgaggcggc cgtccacacc tccgtggcca gggtctcgaa
     5461 gcccgtcggc cgcagtaaca gagtagcggg caattccttc agtgccgtga ggaagacgag
     5521 ggcgaacccg gccagcgcgc cgcgcgagat caagggaagg acgagacggc ggaacgaggt
     5581 ccacggcccg tggccgagcg cccgccccgc ctcctcgatg cgggggctga cctggagcag
     5641 cgcgccgcga aggggcgagg aggcctgcgg gaggaacagc acgacgtaga cgatgacgag
     5701 caggggcagc gactggtaga ggccggggag gtagttcgcg gcgaagaaga cgaacgcgag
     5761 ggcgaccacg atcccgggga gcgcgtagcc caggtaggtg gtgcgctcgg tgagggagac
     5821 ccagcggccg ccgtaccggg ccgcgagcat cgccacgggg aggccggcga cgaccgcgat
     5881 ggcggcgccg gcccccgagg cgacgaccga gctcccggcc ggtcccagga tcggcgtcgt
     5941 ctcgccgccc cccgccaagc cgcggatcat ccagaatccc aggaccgaga ccggcaggac
     6001 cagacccagc gccgcgacga tgccgcacac gacgagggcc ggccaccgcc agcgccccag
     6061 ggggatgggg gagaggggcc gctccgcgcc cccctcggcc cggtagtagc gggcccggcc
     6121 ccgggcccga cgctccagcg agaggatcgc cagtgcgagc gccacgagca tgagcgagag
     6181 gatcgccgcc ggcgtgcggt cgaacgcgcc ccggtactgg aggtagatcg cctgcgtgaa
     6241 cgcggggaac cgcatcagcg agaccgcgcc gaagtcctga acggtgtaga gggcgacgag
     6301 gagggcgccg gcggcgatcg agggccgaag gagggggagg gtgaccgccc ggaacgtccg
     6361 ccaccggctg tgaccgaggc tccgcgaggc ttcctccatg gaggacccga tcccgcggat
     6421 cccagcggcc gtcatcagga agacgtacgg ataggtgaag agggtgaggg cgaggaaggc
     6481 gccggggaag ccgctgatgt ccggaaggcg ctcgacgccc agcggagaca gcagctgctg
     6541 gaacatcccc cggggcccga gggccgagat cagcacgaag gccccgacgt agctcgggaa
     6601 cacgaggggg agggccaaga cgacggacca cacccggcgg aagggcaggt cggtgcgggt
     6661 ggtgagccac gcgagggcga ccccgagcgt caccgcggcg gcggtcaccg cggcggcgag
     6721 cccgatcgtc cgcagggcca gccaggcggt cctgctgcgg aggatcagat cccagctcgc
     6781 ggcgtcggcg tcggccaccc ggatcacgag gtaggcgacg ggaaggacga cgagggccga
     6841 gacggcgagg ccggcggcca ggaggcccca gggagcccgt gtggggcggg gcggccgagc
     6901 ccgcgcagc
//
LOCUS       DASUTZ010000013         5031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1038766, whole genome shotgun sequence.
ACCESSION   DASUTZ010000013 DASUTZ010000000
VERSION     DASUTZ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038766"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="VF097_00485"
     CDS             <1..1191
                     /locus_tag="VF097_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX6261300.1"
                     /translation="AAAPAAATGSPDLEIEIGELFLDPEELSAQPGSLSITVRNTGSV
                     EHNLAIDGVGATPMIPPGGTATLEADDIAAGEYEMICEVPGHADGGMRGTLLVGEAGT
                     GEHDGMAAMTPEEMVAHDAERTGSFPAETEGRGGQPLEPEVLPNGTKVFELTAEAIRW
                     EVEPGKVFDAFAYNGQVPGPELRAELGDRVRIILHNELPQPTTLHLHGMIVPNDMDGV
                     PVINQDAIMPGESFTYEFRVRNTGSNMYHSHFNAQEQVPMGLLGAFIVPDPADPEVDV
                     DATMILNDGPLGFSINGKGFPATEPVVASEGDLIRIRYMNEGLQVHPMHLHGMPQEVV
                     ALDGHILRRPYMMDTVLVAPGQRVDVLVRATEPGPWAFHCHVLTHAEGPDGMFGMVTA
                     LIVD"
     gene            complement(1188..1490)
                     /locus_tag="VF097_00490"
     CDS             complement(1188..1490)
                     /locus_tag="VF097_00490"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HEX6261301.1"
                     /translation="MSAEPKRELQGPRAVLRFIGRSGKRIGVTIVGFLLLLAGGIMMV
                     TPGPGTVAIIAGLAILATEYTWAEIMLDKVKEKAKQAGDAVRKRSRRNKPDPEPPA"
     gene            complement(1556..1972)
                     /locus_tag="VF097_00495"
     CDS             complement(1556..1972)
                     /locus_tag="VF097_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261302.1"
                     /translation="MSRSTKRTKRIALVALIALATACGGDEGSVGPRDVELESVLADP
                     DGHDGERLRFRAFYVGSFELSVLTAALAESYPPQPAEPTIWVEARPRGDCVVSDVGVT
                     WAEVVAEGVFRYEQGGGFGHLGIHRMQLEGARLACP"
     gene            1990..2439
                     /locus_tag="VF097_00500"
     CDS             1990..2439
                     /locus_tag="VF097_00500"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX6261303.1"
                     /translation="MMKNIALIAIMTALLATACEAEVEDPEAGPSPAVTVEDDTAEDD
                     VDVEATPTGCAEVSSAGGAPAPLTMRDSFFEPNCLVVSSTQEMTLVNAGDLPHNFSIR
                     DTDVSEDVDPGEEEESEDLFENLDAGTHEFFCRFHEAEGMVGTIVIE"
     gene            2478..2990
                     /locus_tag="VF097_00505"
     CDS             2478..2990
                     /locus_tag="VF097_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261304.1"
                     /translation="MKRIATVLTLAALAVGCAEVGEDRTPEVGPLATPSPSPGGGATL
                     TLVAAPEGPLARLRGGGQEVEVDAHTSCWDSPGVSTCVDGFPTEPEAYVEVRRGGTLT
                     VSGDAETVEVGVARFRPGDVAAELQVVQDLPLEDGSATIDVEPGRYLLQVFATFEEGD
                     GAFALGLRVL"
     gene            complement(2987..3865)
                     /gene="era"
                     /locus_tag="VF097_00510"
     CDS             complement(2987..3865)
                     /gene="era"
                     /locus_tag="VF097_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX6261305.1"
                     /translation="MTRSGFVAVVGRPNTGKSTLVNALVGQKVSIVSDKPQTTRGRIR
                     GVVTGEGYQVVLTDTPGFHKPRTLLGERLNEMVGESTGDVDAIVLVVDGAAGVGRGDA
                     YVAARQVSPHEVLKVCAVNKVDRLSKPAVLPQLEAASALADFDHVIPVSARTRTGVPG
                     LLRILVEALPEGPFLFPEGEATDQPLERRVAEIVREKALAATREEVPHSIATEVEELE
                     REDGLIRIHCLVLVERESQKGIVIGKGGEMLKRIGTEARRELEALLGEKVYLELRVKV
                     LRDWQRDPKALQRLGY"
     gene            complement(3862..4275)
                     /gene="cdd"
                     /locus_tag="VF097_00515"
     CDS             complement(3862..4275)
                     /gene="cdd"
                     /locus_tag="VF097_00515"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEX6261306.1"
                     /translation="MSSVDQDRVRALLEAAREARDLAYAPYSGFRVGAAVLTADDRLV
                     RGANVENAAYPLSVCAERTAIQRAVTEPGSREVVAVAFVSSGEGETWPCGGCRQVLHE
                     FGPGMVVITEDAQGEPVQRLLAELLPEAFGPADLP"
     gene            complement(4268..>5031)
                     /locus_tag="VF097_00520"
     CDS             complement(4268..>5031)
                     /locus_tag="VF097_00520"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX6261307.1"
                     /translation="AMAEAGSEEGAIEEEEKELIHSIFEFGDTIVREVMVPRPDMVTI
                     GKDASLDHVLDLVLQHGYSRIPVTSNGLDEIAGVVYAKDVLRQLHRGRRDRTVEDLMR
                     KPYFVPESKKVSELLRDMQREKYHLAMVVDEYGAVAGLVTLEDLLEEIVGEIEDEYDR
                     EEPNVEPAGEDRLRVNARLPVDELNELLQVELPNTEWDTVGGLVMGILGRVPAVGEQV
                     EFEGLRFTAERVQGRRIAKILIERLPPEPAEATAE"
BASE COUNT          730 a         1833 c         1728 g          740 t
ORIGIN      
        1 gcggccgctc ccgcggccgc gaccggctcc ccagacctcg agatcgagat cggcgagctc
       61 ttcctcgacc ccgaggagct ctccgcccag cccggttccc tctcgatcac cgtccgcaac
      121 acgggcagcg tcgagcacaa cctcgccatc gacggggtag gggccacgcc gatgatccct
      181 cccggcggca ccgccaccct cgaggccgac gacatcgcag caggtgaata cgagatgatc
      241 tgcgaggtcc ccgggcacgc cgacggaggg atgcggggaa cactcctcgt cggcgaggcg
      301 gggaccggcg agcacgacgg catggccgcc atgacccccg aggagatggt ggcgcacgac
      361 gccgagcgca ccggctcctt ccccgccgag acggagggcc gcggcggcca gcccctcgag
      421 cccgaggttc tgccgaacgg caccaaggtc ttcgagctca cggccgaggc gatccgctgg
      481 gaggtcgagc cggggaaggt cttcgacgcc ttcgcctaca acgggcaggt gccggggccg
      541 gagctccggg ccgagctggg cgatcgggtt cgcatcatcc tccacaacga gcttccccag
      601 cccacgacgc tccacctcca cgggatgatc gtccccaacg acatggacgg cgtccccgtg
      661 atcaaccagg acgcgatcat gccgggcgag tccttcacct acgagttccg ggtgcgcaac
      721 accgggagca acatgtacca ctcgcacttc aacgcgcagg agcaggtccc gatggggctc
      781 ctcggcgcgt tcatcgtccc cgaccccgcg gaccccgagg tggatgtgga cgccacgatg
      841 atcctgaacg acggtccgct cggattctcg atcaacggga agggcttccc ggcaaccgag
      901 cccgtcgtgg cctcggaggg cgacctgatc cggatccgct acatgaacga ggggctccag
      961 gtccacccga tgcatctcca cgggatgccc caggaggtcg tcgcactcga cggacacatc
     1021 ctgcgtcggc catacatgat ggacacggtg ctggtcgcgc ccgggcagcg ggtggacgtc
     1081 ctggtgcggg ccaccgagcc gggcccgtgg gcgttccact gccacgttct gacgcacgcc
     1141 gaggggcccg acgggatgtt cgggatggtg accgcgctga tcgtggacta ggcgggcggc
     1201 tccgggtcgg gcttgttccg ccgagaccgc ttgcggacgg cgtcgcccgc ctgcttcgcc
     1261 ttctccttca ccttgtcgag catgatctcg gcccaggtgt actcggtggc gaggatggcg
     1321 aggcccgcga tgatggccac ggtgccgggc ccgggcgtca ccatcatgat gcccccggcc
     1381 agcaggagca ggaaccccac gatcgtgacc ccgatgcgct tcccgctccg gccgatgaag
     1441 cggaggaccg cccgggggcc ctggagctcc cgcttgggct cagcggacat caggcgtcga
     1501 gcgtcgggag gcgcgctgat ccatgcgggc gatgatatcg aacatccgcg gatcgtcagg
     1561 gacaggccag ccgggcgccc tcgagctgca tcctgtggat ccccaggtgg ccgaagccgc
     1621 ccccctgctc gtaccggaac acgccctcgg cgaccacctc ggcccaggtg acgcccacgt
     1681 cactgaccac gcagtccccc cgcggccggg cctccaccca gatcgtgggc tcggcgggct
     1741 ggggcgggta cgactcggcc agcgccgcgg tgaggacgct cagctcgaac gacccaacgt
     1801 agaacgcccg gaaccgcagg cgctcgccgt cgtgaccgtc ggggtcggcc aggacgctct
     1861 cgagctcgac gtcacgcggc ccgaccgatc cctcgtcgcc cccgcacgcg gtggccagcg
     1921 cgatcagggc gaccagtgcg attcgcttcg tccgtttcgt tgacctgctc atcgggtatg
     1981 cccgcttcca tgatgaagaa catcgctctg attgccatca tgacggccct cctggcgacg
     2041 gcgtgcgagg cggaggtcga ggatccggag gcgggcccga gcccggccgt gacggtcgaa
     2101 gacgacaccg ccgaggacga cgtggacgtg gaggccaccc cgaccgggtg cgccgaggtc
     2161 tcgagcgcgg gcggcgctcc cgccccgctc acgatgaggg actcgttctt cgagccgaac
     2221 tgcctggtcg tgtcctcgac ccaggagatg acgctcgtga acgccggcga cctgccgcac
     2281 aacttctcga tccgggacac cgacgtcagc gaggacgtcg atcccggcga ggaggaggag
     2341 tccgaggacc tcttcgagaa cctcgacgcg ggcacccatg agttcttctg ccggttccac
     2401 gaggcggagg gcatggtcgg gacgatcgtg atcgagtagg cgggaacctc ccggcccgtc
     2461 cccgggtctg acccgacatg aagaggatcg cgaccgtcct cacgctcgcg gcgctcgccg
     2521 tcgggtgtgc ggaggtgggc gaggaccgga ctcccgaggt cggtccgctc gccacgccct
     2581 ccccttctcc cgggggcggg gccacgctga cgctcgtggc cgccccggag ggtcccctcg
     2641 cccggctgag gggcgggggc caggaggtgg aggtcgacgc gcacacctcg tgctgggact
     2701 cgccgggtgt ctccacgtgc gtcgatggct tcccgaccga gccggaggcg tacgtggagg
     2761 tgcgccgcgg cgggacgctg accgtgtcgg gcgacgcgga gaccgtcgag gtcggcgtgg
     2821 ctcgtttccg gcccggggac gtggccgccg agctgcaggt ggtgcaggac ctcccgctcg
     2881 aggacggctc cgccacgatc gacgtggagc cggggaggta cctgctccag gtgttcgcca
     2941 ccttcgagga aggcgacggc gcgttcgccc tcggccttcg ggtgctctag tagcccagcc
     3001 gctggagcgc tttggggtcc cgctgccagt cgcgcagcac cttgacccga agctcgaggt
     3061 agaccttctc gcctaggagc gcctcgagct cccggcgggc ctccgtcccg atccgcttca
     3121 gcatctcgcc gcccttcccg atcacgatgc ccttctggga ctcccgctcc acgagcacga
     3181 ggcagtggat ccggatcagc ccgtcctcgc gctccagctc ctcgacctcg gtggcgatcg
     3241 agtgcgggac ctcctcccgc gtggcggcga gcgccttctc ccggacgatc tcggccacac
     3301 gtcgctccag cggctggtcg gtggcctcgc cctcggggaa caggaacggc ccctcgggaa
     3361 gggcctccac gaggatcctc aggagcccgg gcacacccgt ccgggtccgg gccgagacgg
     3421 ggatcacgtg gtcgaagtcg gccagcgcgg aagcggcttc gagctggggc aggacggccg
     3481 gcttcgacag ccggtccacc ttgttcacgg cgcagacctt gaggacctcg tggggcgaga
     3541 cctggcgggc cgccacgtac gcgtcccccc gccccacgcc cgcggccccg tccacgacga
     3601 ggacgatcgc gtccacgtcg ccggtcgact cgcccaccat ctcgttcagg cgctccccca
     3661 gcagggtgcg gggcttgtgg aagcctgggg tgtcggtgag cacgacctga tagccctccc
     3721 cggtcaccac gccgcggatc ctgccccggg tcgtctgcgg cttgtcggag acgatcgaga
     3781 ccttctgccc cacgagggcg ttcaccaggg tcgacttgcc cgtgttgggg cggccgacga
     3841 cggccacgaa gccggatcgc gtcacgggag gtcggcgggt ccgaacgcct cgggcaggag
     3901 ctcggcgagc aggcgctgga cgggctcccc ctgggcgtcc tcggtgatca cgaccatccc
     3961 gggcccgaac tcgtgcagga cctgacggca tcccccacag ggccacgtct cgccctcgcc
     4021 cgacgagacg aaggccacgg ccactacctc gcggctcccg ggctcggtga cggcgcgctg
     4081 gatcgccgtt cgctcggcgc agaccgagag cgggtaggcg gcgttctcca cgttcgctcc
     4141 gcgcacgagg cggtcgtcgg cggtgaggac ggcggccccc acccggaacc ccgagtaggg
     4201 cgcgtaggcc aggtccctgg cctcccgggc ggcttcgagc agggcccgga cccggtcctg
     4261 gtcgacgcta ctcagcggtg gcctccgccg gctcgggggg caggcgctcg atcaggatct
     4321 tggcgatgcg ccgcccctgc acgcgctcgg ccgtgaagcg caggccctcg aactcgacct
     4381 gctcccctac cgcggggacg cggcccagga tgcccatcac gagaccgccc accgtgtccc
     4441 actcggtgtt ggggagctcc acctggagga gctcgttcag ctcgtccacg gggaggcggg
     4501 cgttcacgcg gaggcggtcc tcgccggccg gctcgacgtt cggctcctcg cggtcgtact
     4561 cgtcctcgat ctcccccacg atctcctcga gcaggtcctc gagtgtgacg agcccggcca
     4621 cggccccgta ctcgtccacg accatggcca ggtggtactt ctcgcgctgc atgtcccgga
     4681 ggagctcgga gaccttcttc gactcaggga cgaagtacgg cttgcgcatc aggtcctcca
     4741 ccgtgcggtc ccgccgcccc cggtggagct gacggaggac gtccttggcg tagacgacgc
     4801 ccgcgatctc gtcgagcccg ttgctcgtca ccgggattcg cgaatacccg tgctgcagga
     4861 cgaggtcgag gacgtggtcc agagaggcgt ccttcccgat cgtgaccatg tcgggccgcg
     4921 ggaccatcac ctcgcgcacg atcgtgtcgc cgaactcgaa gatcgagtgg atcagctcct
     4981 tctcctcctc ctcgatcgcg ccctcctcgc tgccggcctc ggccatcgcc c
//
LOCUS       DASUTZ010000014         4491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_520657, whole genome shotgun sequence.
ACCESSION   DASUTZ010000014 DASUTZ010000000
VERSION     DASUTZ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520657"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VF097_00525"
     CDS             complement(<1..201)
                     /locus_tag="VF097_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007581501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX6261308.1"
                     /translation="MAEAGVGPSPEAPAPELIEAGFRLEVADAPYLHRGLNLADMAHV
                     LVLLEQGIAPPEPAQRLLRLLLE"
     gene            complement(194..892)
                     /locus_tag="VF097_00530"
     CDS             complement(194..892)
                     /locus_tag="VF097_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX6261309.1"
                     /translation="MACVLAMYGDEPETPGANLGLIRGWGAEVRWTGDPDRASVDPAM
                     EKAVAELEAAGRRPYLVPRGGATAIGALGYLDAAGELAGQLEAAGVEPELIVVAAGSC
                     GTLAGLHAGRAAHGLPGRLVGASVSRPLDECEERVLRIATEAAELAGTAGPRASDVSV
                     VDARGPAYAEPSEESDAAARLAADTEGLLLDHVFTAKAMAAFLSMAGDGPSVFVHTGG
                     IVAAIERAGGGGHG"
     gene            complement(855..1076)
                     /locus_tag="VF097_00535"
     CDS             complement(855..1076)
                     /locus_tag="VF097_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261310.1"
                     /translation="MAVRPKVSLAALPTPLKRATRLEEAVGSGPIWVKRDDLTGFALA
                     GNKARKLEYLLGEARRGDGLRPRDVRRRA"
     gene            complement(1067..2422)
                     /locus_tag="VF097_00540"
     CDS             complement(1067..2422)
                     /locus_tag="VF097_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase family protein"
                     /protein_id="HEX6261311.1"
                     /translation="MGARLTDSELYGHLWGTPELREIFDERARLASWVEILATLGEVQ
                     ADLGIVPAEAAAAIRQHASVDRLDLVAVAEETRRTGHSTLGLIRGLRELLPPEGAEWV
                     YYGITVQDLTDTWASLALRRTGEVVWRDLFAIEGTLLELAERTRDLPMAGRTHGQAGA
                     PITLGFKAAGWADEVRRGLDRLREGAPRWLVGQLGGAVGTHGFFGEAGPELRRRFCER
                     LGLADPGISWTASRDRVAEAGTTLASIASTLARIGNEVYQLQRGEIGELREPAGEAVV
                     GSITMPHKRNPEASEHLVSLSRLARAAAGTLLEGQVQEHERDGRGWKAEWVALPEVCL
                     LTGTAAALARTMIERLEIDEEAMARNLGRFAASEAVLAALAPRLGKHRAQELLQEALS
                     RGASAATLEEALRGHPELADVLDDEVLEAARGPAAGSAAAQVDEVLERIREARKDDRW
                     R"
     gene            complement(2407..3282)
                     /locus_tag="VF097_00545"
     CDS             complement(2407..3282)
                     /locus_tag="VF097_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261312.1"
                     /translation="MNLVPRRYRSVSLAEVGSLDPVALRDHFVGREAYRRTEFIVARA
                     GDGLSLLRVEKASVDPLFSPITSVQVLASPDECAVVEDPEVDTAIPTQLGRAALDRAP
                     GARCVVVTGRYEHVSFILDPAPIPVRVVEVTPPHPAKLVDQVRRVLDTAEDLPPVDLR
                     PEEIDIVELARTRPSDRYLYPCRGSGVAPEGAEVAYLDQHPPKEDWVLVGCERSAQIH
                     RWFYDEDAPYVEMCPRELTRASEVPTLTKCCMLEFGVERDGGVVVVPWGASLEEIREG
                     LRIVMEVAEPAWEPV"
     gene            complement(3279..4052)
                     /locus_tag="VF097_00550"
     CDS             complement(3279..4052)
                     /locus_tag="VF097_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HEX6261313.1"
                     /translation="MSRPDFLDLPPRAEKPRAAGLTHLLDKGSPVPDVEALLSVAAGS
                     IDLWKLGWGTAYLDPGVEEKLPLLARHDVRACVGGTLLEAAWLQGRATECLDWAAETG
                     FPCVEVSNGAVGMPIEEKHRLIAEASDRFVVVSEVGSKDPEATVSPAAWAEEVAADLE
                     AGAEWVLTEGRESGTVGLYTPRGEVREDVVEAVTRVAGPARMIFEAPRKSQQAWLIRR
                     FGPEVNLGNVRLDEVLGVEALRLGLRADTIKAPSDEAGS"
     gene            complement(4110..>4491)
                     /locus_tag="VF097_00555"
     CDS             complement(4110..>4491)
                     /locus_tag="VF097_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX6261314.1"
                     /translation="EEYDVLVLPNLYGDIVSDLAAGLVGGLGVAPGGNIGDEAAVFEA
                     THGSAPKYKGMNKVNPMAMMFSGMLMLRHLEERDAADRLEAALAAVIAEGKSVTYDMK
                     PTRDDPTAVGTSDVADAVIEKLQG"
BASE COUNT          569 a         1802 c         1519 g          601 t
ORIGIN      
        1 ctcgagcagc agccgcagca gccgctgcgc cggctcgggg ggcgcgatcc cctgctccag
       61 cagcacgagc acgtgcgcca tgtcggccag gttcagcccg cggtgcaggt agggcgcgtc
      121 ggcgacctcg agccggaacc cggcctcgat cagctcgggc gccggcgcct ccggcgacgg
      181 gcccacgcct gcctcagcca tgacctcctc ctcccgctcg ctcgatcgcg gccacgatgc
      241 cgcccgtgtg gacgaagacc gacgggccgt cccccgccat cgagaggaag gcggccatcg
      301 ccttcgccgt gaagacgtgg tccaggagga gcccctcggt gtcggcggcg agcctcgcgg
      361 cggcgtcgga ttcctctgag ggttccgcgt aggcggggcc gcgggcgtcg acgacggaga
      421 cgtcggacgc ccgcggcccc gccgtccccg cgagctcggc ggcctccgtc gcgatccgca
      481 ggacccgctc ctcgcactcg tcgagtgggc ggctcaccga ggcgcccacc agtcgccccg
      541 ggaggccgtg ggccgcgcgg ccggcgtgca ggccggccag ggtgccgcag gacccggccg
      601 ccacgacgat cagctcgggc tccacgcccg ccgcctcgag ctggccggcc agctcgcccg
      661 ccgcgtccag gtagcccagc gctccgatcg cggtggcgcc ccctcgcggc accaggtatg
      721 ggcggcggcc ggcggcctcc agctcggcga ctgccttctc catggccggg tccaccgagg
      781 cccggtcggg gtcgcccgtc cagcggacct ccgcccccca gccgcggatc aggcccaggt
      841 tggcgccggg cgtctcaggc tcgtctccgt acatcgcgag gacgcaggcc atccccgcgg
      901 cgcgcctcgc ccagcaggta ctcgagcttc cgggccttgt tgccggcgag cgcgaacccc
      961 gtcaggtcgt cgcgcttcac ccagatcggc cccgagccca ccgcctcttc gaggcgcgtg
     1021 gctcgcttca gcggcgtggg gagggcggcc agggacacct tcggcctcac cgccaccggt
     1081 cgtccttccg ggcctctcgg atccgctcca ggacctcgtc cacctgggcc gccgccgacc
     1141 ccgcggccgg acctcgggcg gcctcgagga cctcgtcgtc gaggacgtcc gccagctcgg
     1201 ggtgcccgcg cagggcctcc tccagggtgg cggcgctggc gccgcggctc agcgcctcct
     1261 gcagcagctc ctgggcccgg tgcttgccca gccgcggcgc cagcgccgcc agcaccgcct
     1321 cggacgccgc gaagcggccc aggttccggg ccatcgcctc ctcatcgatc tcgagccgtt
     1381 cgatcatcgt tcgggccagc gcggccgcgg tccccgtcag caggcacacc tcgggcagcg
     1441 ccacccactc agccttccac ccacggccgt cacgctcgtg ctcctggacc tgcccctcga
     1501 gcagcgtccc ggccgcggct cgggccagac gcgacagcga cacgaggtgc tccgaggcct
     1561 cggggttccg cttgtggggc atcgtgatgc tgcccacgac ggcctcgccc gccggctccc
     1621 ggagctcccc gatctcgccc cgctggagct ggtagacctc gttgccgatc ctggccagcg
     1681 tggaggcgat cgaggccagc gtcgttcccg cctccgccac gcggtcccgg gaggcggtcc
     1741 atgagatccc cgggtcggcc agccccagcc gctcgcagaa gcgccgcctg agctcgggac
     1801 cggcctcgcc gaagaaccca tgcgtcccca cggcgccgcc cagctggccc accagccacc
     1861 gcggcgctcc ctcccgcagc cggtccagcc cccggcggac ctcgtcggcc catcccgcgg
     1921 ccttgaaccc gagggtgatc ggcgcccccg cctgcccgtg cgtccgcccg gccatcggca
     1981 ggtccctcgt ccgctccgcg agctcgagga gcgtcccctc gatcgcgaac aggtcgcgcc
     2041 acacgacctc gcccgtcctc ctcagggcga gcgacgccca cgtgtcggtg aggtcctgca
     2101 cggtgatgcc gtagtagacc cactcggcgc cctcgggcgg cagcagctcc cggagtcccc
     2161 ggatcaggcc cagcgtcgag tggccggtgc ggcgggtctc ctccgcgacg gcgaccaggt
     2221 cgaggcggtc cacgctcgcg tgctgccgga tcgcggccgc ggcctccgcc gggacgatgc
     2281 ccagatcggc ctggacctcg cccagcgtgg ccaggatctc gacccacgag gccaggcggg
     2341 ctcgctcgtc gaagatctcg cgcagctctg gcgtccccca gaggtgcccg tacagctcgg
     2401 agtcggtcag acgggctccc acgccggctc ggccacctcc atcacgatcc ggagcccctc
     2461 gcggatctcc tcgagggagg cgccccacgg gacgacgacg acgcctccgt cgcgctccac
     2521 cccgaactcg agcatgcagc acttggtcag ggtcgggacc tccgaggccc gggtcagctc
     2581 gcgcgggcac atctcgacgt agggagcgtc ctcgtcgtag aaccaccggt ggatctgggc
     2641 ggaccgctcg caccccacga gcacccagtc ctccttgggg ggatgctgat cgaggtaggc
     2701 cacctcggcc ccctcgggcg ccacgcccga tccgcggcac gggtagaggt agcggtccga
     2761 cgggcgcgtc ctggccagct ccacgatgtc gatctcctcg gggcgcaggt cgaccggggg
     2821 gaggtcctcc gcggtgtcca gcacccggcg cacctggtcc acgagcttgg cggggtgggg
     2881 cggggtcacc tcgacgacgc ggaccgggat cggggcgggg tccaggatga agctgacgtg
     2941 ctcgtaccgc ccggtcacca cgacgcatct cgcgcccggg gctcggtcca gggccgcccg
     3001 tccgagctgg gtcgggatcg ccgtgtcaac ctccgggtcc tccaccacgg cgcactcgtc
     3061 cgggctcgca agcacctgca ccgacgtgat cggagagaag agcggatcga ccgacgcctt
     3121 ctccacgcgc agcagcgaga ggccgtcccc ggcccgggcc acgatgaact cggtgcgccg
     3181 ataggcctcg cgaccgacaa agtggtcccg gagggccacc gggtccaggg accccacctc
     3241 ggccagggat acgctgcggt accgccgagg gacgaggttc acgagcccgc ctcgtcggac
     3301 ggcgccttga tcgtgtcggc gcggaggccc agccggagcg cctccacgcc gagcacctcg
     3361 tccagacgga cgtttcccag gttgacttcg ggtccgaacc ggcggatcag ccacgcctgc
     3421 tgagacttcc ggggagcctc gaagatcatc cgcgcgggcc ccgccacccg cgtcacggcc
     3481 tcgaccacgt cctcgcgcac ctccccccgc ggcgtgtaga ggccgacggt gccgctctcg
     3541 cgcccctcgg tcaggaccca ctcggccccg gcctcgaggt ccgccgcgac ctcctcggcc
     3601 caggcagccg gcgagaccgt cgcctcgggg tccttggacc ccacctcgga cacgacgacg
     3661 aagcgatccg acgcctcggc gatcagacgg tgcttctcct cgatcggcat gcccacggcg
     3721 ccgttcgaca cctcgacgca cgggaacccc gtctccgcgg cccagtccag gcactccgtc
     3781 gccctgccct ggagccacgc cgcctccagc agggtccccc ccacgcacgc ccggacgtcg
     3841 tgccgagcga gcagcgggag cttctcctcc accccggggt cgaggtaggc ggtgccccac
     3901 ccgagcttcc agagatcgat cgaccccgcg gccacggaga gcagggcctc gacgtcgggc
     3961 acggggcttc ccttgtccag gaggtgggtg aggccggcgg cccgcggctt ctccgctcgg
     4021 ggcgggaggt cgaggaagtc gggcctgctc acggtgtctc ctatggtaga cgctcgtcca
     4081 tcctggtcca acccggaccg ggccggcgct cagccctgga gcttctcgat caccgcgtcg
     4141 gcgacgtcgc tcgtccccac cgccgtgggg tcgtcccggg tgggcttcat gtcgtacgtg
     4201 acgctcttcc cctcggcgat caccgcggcc agggcggcct cgagccggtc ggccgcgtcc
     4261 cgctcctcga ggtgccgaag catcagcatc cccgagaaca tcatcgccat cgggttgacc
     4321 ttgttcatcc ccttgtactt cggggcgctc ccgtgggtgg cctcgaagac ggccgcctcg
     4381 tccccgatgt tccctcccgg cgccacgccg agccccccga ccaggcccgc ggccaggtcc
     4441 gacacgatgt cgccgtagag gttcgggagg acgagcacgt cgtactcctc c
//
LOCUS       DASUTZ010000015        22385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_262123, whole genome shotgun sequence.
ACCESSION   DASUTZ010000015 DASUTZ010000000
VERSION     DASUTZ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 22385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22385
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262123"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VF097_00560"
     CDS             <1..328
                     /locus_tag="VF097_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HEX6261315.1"
                     /translation="ISETQRRRRIQLEHNREHGIDPQTIRKKVSDILLTMVEERPETA
                     PKRRRRAPERPELPREELTRLIESLQEEMHLAAKELKFEYAARLRDEVNELRKELRAL
                     DEIGVR"
     gene            481..1884
                     /locus_tag="VF097_00565"
     CDS             481..1884
                     /locus_tag="VF097_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261316.1"
                     /translation="MRPRFRWPVIAFASLALMTTALPAGADHETFPSSNSMHALGHSV
                     HEATFAVPDGQRKISSDLAFWGKIAYQGNYDGFRVLDVSAPGNPKLISHPSCNGDQGD
                     IFVWDDVLVRSWNSPAPPGRTCMGEPVPIDFEGVHVFDVSDPADPELLTAVEFSAVGA
                     LMRGTADGCGSHTLTGAADLENNRLIVYSNNSSGGGRPVCDAIDIIEVPLDDPASASH
                     IGKVPLTGGSLGSVNGCHDAGLILGDVNLLACASGHAANVFSVGAPRGGSLENPQFLY
                     TVEETDEMGNQVGIGGRWHSAGFTWDGEVLVLGWEPGGGGAAECEAGDADIKKSLFFY
                     DADTGEKLGQWVLERPQGADENCTIHNYNVVPLRSGRYVAVMGNYQAGTWVVDFTDPA
                     NAESVAWADPESLGPGPFCGGECHIGGAWSSYWYNGSIFESDITRGLAVYKASHRSLA
                     GAIRVPHSNPQTQEFSL"
     gene            1967..3028
                     /locus_tag="VF097_00570"
     CDS             1967..3028
                     /locus_tag="VF097_00570"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6261317.1"
                     /translation="MTLLVQGSLAWRGPEEPLVEDAAVVLDGSRIVYSGSARLAPEAE
                     EEVVGDWFLMPAAVDHHVHIGLADPRAVLAGGVTQVRDLGWPPEDVFPLADISTGTDF
                     DGPAIAACGPIVTAVGGYPTRSSWGPPGIAAEVSGPEEAAAAVAGIAEQDPAAIKVAL
                     NTEAGPSLTDAELVAVCEAAHERRLPVVAHVEGPGTTERALGAGVDELAHTPWIERLS
                     EAVVTGLVRRGVRMVSTLDIHSYGKVTPDLRSAVENLFRFHSAGGTVLYGTDLGNGPI
                     PPGIDVREAQHLAAARMDVEEILTAMTAWSLHPGARGDLIALDGNPFEELDALGHVAL
                     VVKGGRVRSLEGRGPREEA"
     gene            complement(3092..3553)
                     /locus_tag="VF097_00575"
     CDS             complement(3092..3553)
                     /locus_tag="VF097_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261318.1"
                     /translation="MAIVPVVLALGVSPALAQSGHFITGGGNAPSCTDIGTQVECTGK
                     VAGLGGTTFEITVEASGIAEVVCINPGGNRAPGQDTEVTVAGTTEPLPTPKNGQFRFS
                     ITSDDPEPLPPTPTCPNAQWTPEIVDVAFTTATLTLFEDGMQSDQVIVTVM"
     gene            3952..4554
                     /locus_tag="VF097_00580"
     CDS             3952..4554
                     /locus_tag="VF097_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6261319.1"
                     /translation="MNAVHRVLVVGRAAIRDVPTRLFRRAGYDIGEVEDGEAALVELE
                     ERHPHLVVVDRDLPDMSGYELTYEVRERFGADPSVIMLSETRDEPHDKIAGLLIGADD
                     YLYVDESFRSDELLARARRLLADPVPSERHAYRLTPREIEVLQHLASGLGREVMAKKL
                     YISPKTVATHIQHILTKLGVRSTAEAVAFAYRNGLVDEQP"
     gene            4594..7443
                     /gene="uvrA"
                     /locus_tag="VF097_00585"
     CDS             4594..7443
                     /gene="uvrA"
                     /locus_tag="VF097_00585"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX6261320.1"
                     /translation="MGTEKLVIHGAREHNLKNVHLELPRNELIVFTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMEKPDVDFIEGLSPAISIDQKSTSKNPRSTVGT
                     ITEIYDYLRVLYARIGIPHCPRCGRPIGRQTTDQIVDQVAALPEGTRFQVLAPIVRGR
                     KGEYEKLLKDLARQGFARARIDGEVRDLSEPIRLDRYYKHDIEVVVDRLVAKADVRRR
                     LADSIETALNLAEGLAAIAIRTDGTEEVVTFSQSFACTSCGLSFDEPAPRNFSFNSPY
                     GACPTCDGLGTRLEVDPELVVPDPDRTLENGALAPWNSTRLEYWNRMLASVCEAHGIP
                     TDVPWRKLAKEQRKTLLYGSDDEVHIRYRNRYGRVRSYWTTYEGIIPNLERRHDETES
                     EFSRDRIEQYMREVPCRTCRGARLKAESLAVTVGELNISELTAKSVGKALTFLLELEE
                     NLSDRERMIAERLLREIRSRLGFLVDVGLDYLTLDRAAATLAGGEAQRIRLATQIGSG
                     LVGVLYILDEPSIGLHQRDNQRLIDTLVRLRNLGNTLIVVEHDEATIRTADWIVDIGP
                     GAGAAGGEIVYSGEVKGLFEHPGSLTGEYLSGEREIPVPSVRRSASGWLEVKAAAEHN
                     LKGIDVRFPLGTFICVTGVSGSGKSTLVEDVLSRALQQKIQGSRVVPGKHRRLVAWER
                     VDKLVVVDQSPIGRTPRSNPATYTGVFDHIRRLYAQVPEARVRGYQPGRFSFNVRGGR
                     CEACSGEGQIKIEMHFLPDVYVTCEQCKGRRYNRETLEVRYKHRTIADVLDMSVDEAL
                     AFFQNQPTIARYLQTLSDVGLGYLRLGQPAPTLSGGEAQRVKLASELQRRSTGSTFYI
                     LDEPTTGLHFEDVRKLLGVVERLVAAGNTVVVIEHNLDVVKTADWLIDLGPEGGDAGG
                     EIVTEGTPEEVAAAPGSHTGSFLAQVLGRERAAAG"
     gene            7492..9051
                     /locus_tag="VF097_00590"
     CDS             7492..9051
                     /locus_tag="VF097_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261321.1"
                     /translation="MIRGRHPLLALVAVVAASCGPGDVRVSSVPIEEVGFVTQIVDVQ
                     GEAGAGISIAADAEGSPHLAYLAFERELEEGEQPPPPDPNLPLFPAVRHAHQAQGVWS
                     RGTAAEELGDLTQGDGTAIAVDGDGMEHIAWTEGGRLRYSSNGGGAFSEPETVVRADE
                     LHGVAIAAAEDGTPSISFYDVPGGPEGPAALVRVATPAGGGWEVETAAEADPNQPMST
                     GIGITQNGAIVAYGSGPTTYVARDLEDRWESEEVDAGGLGVSLDLDADGNPHVSYYTS
                     DGTVRHAHSVGGGPWETSDVGSAGQGPGSDWVTSISVDPEGVHWVAWQTAEGLGLASN
                     EEGSFQEHDLPGTQGGVRPQVATGAEGAVYVAWHDTEDGTANVSSRTSEEPLLAGPPE
                     TGAPQPGPTGDGGGGGGGDGGPPPCEPSGTELAIVAPVGAVADGFDTDCLAAPAGEAF
                     TIDFDNQDTGVPHNVSIYADDTAAERFFEGEIITGPDQITYEPDPIDDAGVFYFHCDV
                     HPNMNGAFVVQ"
     gene            9072..10931
                     /gene="uvrC"
                     /locus_tag="VF097_00595"
     CDS             9072..10931
                     /gene="uvrC"
                     /locus_tag="VF097_00595"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX6261322.1"
                     /translation="MTTLRRPETSTIPDDPGAYLFRDADGRVIYAGKAKSLRKRLVTY
                     WAKPQHPRTQAMLDAAAAVEWIVASGEVDALMLEYNLIQRHRPRFNVRYRDDKSYPYL
                     AVTVGETWPRAYVMRGKRRKGVRRFGPYGHAYAIRDTLDSLTRVFPVRTCSNGFFDQR
                     RRARRPCIYYDIGRCLGPCVPERTGVTEEGYRAEVEALMDFLDGNYRPVLDRLDEEMR
                     GAAERQEFEAAARFRDRIAAARRAMEQQEMVLGRPQDLDVIGMVEDDLEAGFQVFFVR
                     RGRVMGRRGWVADRVEDLDTPALVSSLVRELYMERDEVPPRVLVPAWPLDRQVLERWL
                     GERRGGRVEITVPRRGEKRRLQQVVTTNAKEAFVRHKLQRATDFASRSRALDELGRAL
                     GLEDAPLRIEAYDISNLVGTEKVGSMVVFEDGLPKRSEYRRFRIKGVPGQDDVASMEE
                     TLRRRFARYLEERERPASERKRFAYPPSLVVVDGGRGQLGAAVRVLNELGLEIPAAGL
                     AKRLEEVYLPGKPDPLRIPRGSEALYLLQHVRDEAHRFALDLHRKRRERRALASPLDE
                     VPGIGPARKKALLRRFGSLKRLAKASPDDIAEVPGVGPALAQQVHEALTRRSA"
     gene            10928..11854
                     /gene="rapZ"
                     /locus_tag="VF097_00600"
     CDS             10928..11854
                     /gene="rapZ"
                     /locus_tag="VF097_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX6261323.1"
                     /translation="MSGRRARAREHRAAREQARQRALQTDFTIITGLSGAGRSEAAKC
                     LEDLGYFVVDNLPPALIGKMAELVAQSEEPRRVAIVADARGGVFFADLAKGLEELREA
                     GTPARILYLEADDETLVMRFAATRRRHPLAPADRMVEGIRKERDLMEALREEADLIID
                     TTGLTPNGLRERIRDTFAAARAEDGLQVAVVSFGYKHGVPRDADLVLDCRFLPNPHWI
                     EELRPLPGTDEAVRSYVISQHAYGEFMKRLRSLLDFVLPGYVAEGKSYLTIAIGCTGG
                     RHRSVVVAEEIAGHLESQERRVALEHRDLERE"
     gene            11923..12927
                     /gene="gap"
                     /locus_tag="VF097_00605"
     CDS             11923..12927
                     /gene="gap"
                     /locus_tag="VF097_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013154027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX6261324.1"
                     /translation="MGVKVGINGFGRIGRNFFRAAKQRGLDLDFVGVNDITDAATLAH
                     LLKYDSVLGTLDADIQASEGGITVDGDEIRVLAERDPGALPWKDLGAEVVVESTGLFT
                     KREDAEKHLSAGAQKVVISAPAKGEDITIVLGVNHEAYDPSSHHVISNASCTTNCVVP
                     MAKVLADAFGIDRALMTTIHAYTNDQNILDLPHKDLRRARAAAINIIPTSTGAAKATS
                     LAIPELKGKMDGVAMRVPVPDGSLTDLVCVLEQEPSVDEVNEAFAAAGSGPLQGILRY
                     SEDPIVSSDIVGDPHSCIFDAPSTMAIGNLVKVLGWYDNEWGYSNRLVDLVEMVGSSL
                     "
     gene            12930..14126
                     /locus_tag="VF097_00610"
     CDS             12930..14126
                     /locus_tag="VF097_00610"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX6261325.1"
                     /translation="MGRLRGIEDLGDPSGRRVFVRADFNVPLEGGRVADDLRIRATLP
                     TIEELRGRGAALVLASHLGRPKGEPRAELRMDPVARRLEELAGAPVTKVDEVVGPEVE
                     KAAHALDPGGLLLLENLRFDPGEEANDPGLARRLAGLADAYVNDAFGAAHRAHASVVG
                     IAEHLPSAAGRLMAREIEVLSRLLEGPERPFVAILGGAKVSDKLGVVRSLMERVDALL
                     IGGAMAFTFILAQGGQVGSSMVEAERVGEVRETMEAASRRGVLIVLPEDVVAAAQPAQ
                     DARKQTVRAGEVPAGLMGLDIGPRSVEEFARVLADARTIFWNGPMGVVELEPFSLGTR
                     GVAQAVAGAGAFSVVGGGDSIAAIRRLGFEDDVDHLSTGGGASLEFLEGRELPGVAPL
                     MEENDG"
     gene            14119..14889
                     /gene="tpiA"
                     /locus_tag="VF097_00615"
     CDS             14119..14889
                     /gene="tpiA"
                     /locus_tag="VF097_00615"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017535440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX6261326.1"
                     /translation="MAERRPIIAANWKMHKTHLEAIETVQKLSYLLDEDDTERVEVVI
                     CPPFTALRSIQTVISGDRLPFGIGAQDVHWEEKGALTGEVSPPMLAALDCAYVIVGHS
                     ERRQHFGETDADVARKARAALAAGIVPIVCVGETLEERDAGRTEEKVTRQVREALAKV
                     PPDRAGELVLAYEPIWAIGTGRAATPQDAGEVIAAIRATLSSMFAHEVAEAVRVQYGG
                     SVKAGNIREFMAHPEVDGALVGGASLDPEELALIVRYR"
     gene            14886..16145
                     /gene="apgM"
                     /locus_tag="VF097_00620"
     CDS             14886..16145
                     /gene="apgM"
                     /locus_tag="VF097_00620"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX6261327.1"
                     /translation="MKKLFYVCLDGLGDEPLPALGDRTPLEAASTPLLDDLAARGRQG
                     LVHTVGPDVAPESDIAVFAILGYDPREEHPGRGVLEALGSGMAFEDGDLAYRVNFATA
                     EHGRIVDRRVGRDLSSEEASALADEVNREVELPGAASELRATVEHRGALVIRASDGPL
                     SAQVSNTDPAYAKVGPLGVALETFGDRPLPAQPLDDTEVARRSAELTNAWTERAGAIL
                     EASEVNTGRRAGGRLPANLVLARDAGDHIPRLVPIKERFGPSWGCFVEMPVERGIAMI
                     LGMGEVPAPTPADPAERYAAWGQMALEAIGGYDALYIHIKGPDVPAHDGRAEDKRDVI
                     ETIDAAFFGTLLPGIDPGGTILAVTADHATSCARKAHTADPVPLIVAGGGVDPDGTTA
                     FGERAAADGSIGTIPGVQIVPTLVDLL"
     gene            16278..17351
                     /locus_tag="VF097_00625"
     CDS             16278..17351
                     /locus_tag="VF097_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6261328.1"
                     /translation="MSQVATEPIDESGLGPGPGGEPGQIVGRSPWELFWRRFRQDRLA
                     TAGIIIIAIIAALALTAPLFSRLVGHDPNQTFAQEYPAGALDDFGLPIGPNKDFWFGA
                     DTAGRDLFVRVLYGAQTSLSVALLATAISLVIGVVGGLFAGFYRGKVDTTVSRLIDIV
                     LAMPVLLLALGLVAACGTAEGCLGGLWGFLKPGWRLVALIIGLFSWPYIARIVRGQVL
                     SLREKEFVEAARSMGSSDRRIIFREVLPNVVAPIIVYTTLIIPNNILFEAALSFLGVG
                     VPPTTPSWGRMLAEGGSNFTFAWWMMVFPGVFLFLTTLAFNLVGDGLRDALDPKMSSV
                     RRKKSKRQAAQEASAEAEASLAG"
     gene            17415..19274
                     /locus_tag="VF097_00630"
     CDS             17415..19274
                     /locus_tag="VF097_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6261329.1"
                     /translation="MSRKHLKLIALAGVVALAAAACGGGDTGGTGEDDQQEEEVQKGG
                     TLQLNLLSDVISAFDPQKEYYSVTWEFYRCCLLRTLMSYNGMSTEEGGTEIQPDLATA
                     MPEVSDDGLTWTFSIREGVNYAPPFDDTEIVAQDFVRALERLADPDASAGGYAFYYSV
                     IEGFDDFSAGEADSISGLNVVDDHTLEVTLTDPAGDFGYRMAMPATAPIPEGAADGHV
                     QDYGRFLVASGPYMFEGSENLDFSAAPNQQEPVSGYVPGQSITFVRNPSWSNEIDDLR
                     PANVDRIEITIGGTEEDNANKVDSGELDMQFDGVAPPEQVRKYQTDPQLEDQLFVNPS
                     DAVRYLTINLAMPPFDDIHVRKAINLALDKDSMRRIRGGPLVGELAGHIMVDSLQNNL
                     LVDYDPYETPNGQGDIEAAQAEMAQSQYDTDGDGVCDDPVCENIPTNTDEADPYPDQA
                     ALIQQNFEPLGLTLDIRQFERGTMYDRVNDPNTQTALGLAPGWGKDYLDGTTFGEPLF
                     GAASIGPDACCNYSLVGATAEQLEGFGYDVTEAPSVDDKIDECEAATDDARFQCWAEL
                     DQELMENVVPWVPYLFDNYVRTVSERVENYTFDQFAGLPALDHIALAGGGAES"
     gene            19347..20351
                     /locus_tag="VF097_00635"
     CDS             19347..20351
                     /locus_tag="VF097_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6261330.1"
                     /translation="MGRYLIRRTLFLVLVLFIISVLTFLIFVKLPPGDPARRFAGRRA
                     TAQQIQQVREQLGLNRSVFVQYWSLAKGLIPWPPQSVLTADPDAGLFLNRDSYYSYVN
                     FVPVKEEIYRRLPVTITLALGGAVVWVLIGLPVGIVSAVRPRSLVDRSGMIFALFGVS
                     APVFWLGFVFLYIFWFRLGILPGSGLEQGQTLVENVLAGRFIMPWVVISLSFAAFYAR
                     MVRGNLIETMGEDYIRTARAKGLSERRVILRHGLRSALTPVVTMFGMDMGILLGGAII
                     TETVFNLPGIGQYAIRSLLTNDIPAVMGVTVLAAFFIVVANLVVDIVYAFLDPRVRYT
                     "
     gene            20385..21398
                     /locus_tag="VF097_00640"
     CDS             20385..21398
                     /locus_tag="VF097_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6261331.1"
                     /translation="MAEPLLEVKDLRVHFPTEDGLVKAVDGVSFTVQPGETLGVVGES
                     GSGKSVTFLTVLGLVSRREAEISGQILFRGKDLLQTPEDELREFRGSQASMIFQDPMT
                     SLHPYYKVGKQIVEAILTHEKISKREAMDQSVEMLRRVGIPRPEERARQYPHEYSGGM
                     RQRAMIAMALALNPDLLIADEPTTALDVTVQAQILDLIDRLKEEFGAAVVIITHDLGV
                     VAEHCDNIMVMYAGKPAEYGTRQDIYYDAHHPYTWGLLGSITRLDQLRSDRLRPIPGL
                     PPSLIQVPPGCPFHPRCPYVMDVCKVEVPQLLADDSGSHRSACHLPLTEKERIFQEEV
                     ALT"
     gene            21428..>22385
                     /locus_tag="VF097_00645"
     CDS             21428..>22385
                     /locus_tag="VF097_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX6261332.1"
                     /translation="MAPEPDSSRTPLVKVVGLKKHFPITRGVLFQRKVGAVHAVDGID
                     LEVYPGETVGLVGETGCGKSTTARLVMKLLEATEGQVFYDGQDITRYGRRRMRELRGE
                     MQMIFQDPFASLNPRKTVGSIIGEPFRVHGTTPKAGIKREVQILMELVGLNPEHYNRY
                     PHEFSGGQRQRIGVARALALRPRLIVADEPVSALDVSIQAQILNLLEDLQEEFNLTYL
                     FIAHDLSVVKHMSDRVAVMYLGKIVEIAPGETLYRNPKHPYTGALLSAIPIPDPKAAE
                     QRRRIVIEGDVPSPIDPPSGCRFHPRCPKAQFPTCSDIEPLLE"
BASE COUNT         3447 a         7637 c         8004 g         3297 t
ORIGIN      
        1 gatctccgag acccagcggc gccgcaggat ccagctcgag cacaaccgcg agcacggcat
       61 cgacccgcag acgatcagga agaaggtttc cgacatcctc ctcaccatgg tggaggagcg
      121 gcccgagacg gcccccaaga gacgccggcg ggcccctgag cgcccggagc ttccccgcga
      181 ggagctcacg cgtttgatcg agagcctcca ggaggagatg cacctcgcgg ccaaagagct
      241 gaagttcgag tacgcggccc ggctccgcga cgaggtcaac gagctccgca aggagttgcg
      301 ggcgctcgac gagatcggcg tccggtagcg ggttcgctcg cgtcgatcct ggggtcgggt
      361 ttgcggaacc gggggcccat tgctagggtc tgggccttgg gaggtcctct cgcgccgcag
      421 ggaggtgatg ccctcgaccc acgcgacgcc catcggacgc atcaacgcaa aggaggaccc
      481 atgcgcccaa ggtttcgatg gccggtgatc gcgttcgcgt cgctggccct gatgacgaca
      541 gcgctccccg cgggcgccga ccacgagacg ttccccagct cgaacagcat gcacgccctg
      601 ggtcacagcg tgcacgaggc cacgttcgcg gtgccggacg ggcagcggaa gatcagctcg
      661 gatctcgcct tctggggaaa gatcgcctac caggggaact acgacggctt ccgggtgctc
      721 gacgtctccg cgccgggaaa ccccaagctg atctcccacc cgagctgcaa cggtgatcag
      781 ggcgacatct tcgtctggga cgacgtcctg gtgcgctcct ggaactcccc cgcgcccccc
      841 ggccgcacgt gcatgggtga gccggttccg atcgacttcg agggcgtcca cgtcttcgat
      901 gtgagcgacc cggcggaccc ggagctgctg acggccgtgg agttcagcgc ggtcggggcg
      961 ctcatgcggg ggacggcgga cgggtgcggc tcgcacacgc tgacaggcgc cgcggacctc
     1021 gagaacaatc ggctgatcgt gtacagcaac aactcgtcgg gcggcggccg gcccgtgtgt
     1081 gacgccatcg acatcatcga ggtgccgctg gacgaccccg cgtcggccag ccacatcggc
     1141 aaggtgccgc tcacgggcgg ttcgctcggc tccgtgaacg ggtgccacga cgccggcctg
     1201 atcctgggtg acgtgaacct gctggcctgc gccagcggcc acgccgccaa cgtgttctcg
     1261 gtgggcgccc cgcgcggcgg cagcctggag aacccgcagt tcctctacac ggtcgaggag
     1321 accgacgaaa tggggaacca ggtcgggatc ggcggccggt ggcactccgc cggctttacc
     1381 tgggacggcg aggtgctggt gcttggctgg gaaccgggcg gcggcggcgc cgcggagtgc
     1441 gaggctggcg acgcggatat caagaagagc ctgttcttct acgacgcgga taccggcgag
     1501 aagctcggcc agtgggtgct ggagcgcccg cagggagcgg acgagaactg cacgatccac
     1561 aactacaacg tcgtcccgct ccggagcggc cggtacgtcg ccgtaatggg taactaccag
     1621 gccgggacct gggtcgtcga cttcaccgac ccggcgaacg ccgagtccgt ggcctgggcg
     1681 gatccggagt ccctcggccc ggggccgttc tgtggcggtg agtgccacat cggcggtgcc
     1741 tggtcctcgt actggtacaa cgggtcgatc ttcgagagcg acatcacgag ggggctcgcc
     1801 gtgtacaagg cgagccaccg gtcgctggcg ggggcgatcc gcgtcccgca ctcgaacccg
     1861 cagacccagg agttcagcct ctagggaagc gggtggagcg gaggggcggg cggccgggct
     1921 ccggccgccc gcccctccgg cgtgctcgga ccgatatcgt gtctccgtga cgctgctcgt
     1981 gcaggggtcg ctggcgtggc gggggcccga ggagcccctg gtcgaggacg ccgccgtggt
     2041 cctcgacggc tcccggatcg tctactcggg ctcggcccga ctcgcgcccg aggccgagga
     2101 ggaggtggtt ggcgactggt tcctgatgcc cgcggcggtc gaccaccacg tgcacatcgg
     2161 gctggcggac ccgagggcag tgctggccgg aggggtgacc caggtgcggg atctgggctg
     2221 gccgcccgaa gacgtgttcc cgctggccga catctccacg ggcaccgatt tcgacggacc
     2281 cgccatcgcg gcgtgcggcc cgatcgtgac ggccgtcggc ggctacccca cacgatcgtc
     2341 gtggggcccg ccggggatcg cggccgaggt gagcgggccc gaggaggctg ccgcggccgt
     2401 ggccggtatc gccgagcagg atcccgcggc gatcaaagtg gcgctcaaca ccgaggcggg
     2461 cccttcactc accgacgcgg agctcgtcgc cgtctgcgag gcggcccacg agcgccggct
     2521 ccccgtcgtg gcgcacgtgg aaggccccgg gaccaccgag cgggcgctgg gcgccggggt
     2581 ggacgagctg gcccacacac catggatcga gcggctgtcc gaggcggtcg tgacgggcct
     2641 cgtgcgacgg ggcgtccgca tggtgtcgac gctcgatatc cactcctacg ggaaggtgac
     2701 ccccgacctc cggtccgcgg tggagaacct cttccgcttc cacagcgccg gtggaaccgt
     2761 cctgtacggg acggatctcg gcaacggccc catcccgccc gggatcgatg ttcgcgaggc
     2821 ccagcacctg gcggccgccc ggatggacgt cgaggagatc ctcacggcga tgaccgcctg
     2881 gtcgctccat ccgggggccc gcggggatct catcgcgctc gatgggaacc ccttcgagga
     2941 gctggacgcg ctgggacacg tggcgctcgt ggtgaagggg ggccgtgtcc gatcgctgga
     3001 gggccgtggg ccgagggagg aggcgtgagg ccactccagc atggaggaag gggccggcct
     3061 ggatggccgg ccccttcgtc aagatgcagc gctacatgac cgtcacgatc acctgatcgg
     3121 actgcatgcc atcctcgaag agcgtgaggg tggccgtcgt gaaggcgacg tcgacgatct
     3181 cgggcgtcca ctgcgcgttc gggcacgtgg gcgtgggggg cagcggctcg ggatcatcgc
     3241 tggtgatgga gaagcggaat tgtccgttct tcggggtggg gagtggctcg gtcgtgcctg
     3301 caacggtcac ctcggtgtcc tgccccgggg ctcggttgcc gccggggttg atgcagacga
     3361 cctcggcgat ccccgaggcc tccaccgtta tctcgaaggt cgttcccccg agcccggcca
     3421 ccttgcccgt acattccacc tgtgtcccga tgtccgtgca gctcggggcg ttgccgcccc
     3481 ccgtgatgaa gtgcccgctc tgggccagcg ccgggctcac cccgagcgca agcaccacgg
     3541 gcacgatggc catcgtcaag agcatccttc gcatggtgat ccttcctctc gaactccgcg
     3601 ggcaacacga acctgcggtt cggggatgca tcaccccttg cgcaagggtt gggaccagag
     3661 cggcgcctca cctcctttcg ctgcacgtcg tggggacgat tgttcgagaa ccaggccgtc
     3721 cggtcatcgg gacggggcgg tatctggccc tagggaggcc ggtcagttcc gtgtaggtag
     3781 cggatctcca taggcctgcc ggttcgccaa tcaggtcgct gcgatcggcc ctcttccgca
     3841 ccacgtccgt tcgagtccgc tcggacattt gacccacatg gagtaaggtg atatggccta
     3901 cgacatgacg taggtctgat ccgcgcaggg aggagccgct ccacggatct catgaacgct
     3961 gtccacaggg ttctcgtggt cggcagggcg gccatccgtg acgtccccac ccggctcttt
     4021 cgtcgagccg gctacgacat cggtgaggtc gaagacggcg aggcggcgct cgtcgagctg
     4081 gaggaacgcc acccacacct ggtggtcgtc gatcgggacc ttcccgacat gtcggggtat
     4141 gaactcacct atgaggtcag ggagcgattc ggagccgacc ccagcgtcat catgctctcg
     4201 gagacgcgcg acgagccgca cgacaagatc gccggcctgc tcatcggggc ggacgactac
     4261 ctctacgtcg atgagtcctt ccgctccgat gagctgctgg ctcgagcccg ccgcctgctc
     4321 gccgacccgg tcccaagcga gcgccacgcg taccgcctga ccccgaggga gatcgaggtg
     4381 ctccagcatc tcgcctcggg cctggggcgc gaggtcatgg cgaagaagct ctacatcagc
     4441 cccaagaccg tggcgaccca catccaacac atcttgacca agctcggtgt acggagcaca
     4501 gccgaggcgg tcgcgttcgc ctatcggaac gggctcgtgg acgagcagcc gtagctggga
     4561 cctccgaggg cgagccgccg ctacaatgcc ttcgtgggca ccgagaagct ggtcatccac
     4621 ggtgcccgtg agcacaacct gaagaacgtc cacctcgagc tccccaggaa cgagctcatc
     4681 gtgttcacgg gcctgtcggg ctccggcaag tcgtcgctgg ccttcgacac gatctacgcc
     4741 gagggtcagc gacggtacgt ggagtccctc tcggcctacg cccgccagtt cctggggcag
     4801 atggagaagc ccgacgtcga cttcatcgag gggctctcgc cggcgatctc gatcgaccag
     4861 aagtcgacct cgaagaaccc ccgctccacc gtgggcacga tcaccgagat ctacgactac
     4921 ctccgcgtgc tgtacgcacg catcgggatc ccgcactgcc cgcgctgtgg acgcccgatc
     4981 ggtcgccaga ccaccgacca gatcgtcgac caggtcgccg cgctccccga gggcacgcgc
     5041 tttcaggtgc tggccccgat cgtccggggg cggaagggcg agtacgagaa gctgctgaag
     5101 gatctggccc gccaggggtt cgcccgggcg cgcatcgacg gcgaggtccg ggacctctcc
     5161 gagccgatcc ggctggatcg ctactacaag cacgacatcg aggtggtcgt ggaccgcctc
     5221 gtggccaagg ccgacgtgcg gcggcggctg gccgactcga tcgagacggc gctcaacctc
     5281 gccgagggcc tggccgcgat cgcgatccgc accgacggga cggaggaggt cgtgaccttc
     5341 tcccagtcgt tcgcctgcac gagctgcggg ctgtcgttcg acgagccggc gccgcggaac
     5401 ttctcgttca actcgcccta cggggcctgt ccgacctgcg acgggctcgg cacgcggctc
     5461 gaggtcgatc ccgagctcgt cgtgcccgac cccgaccgga cgctcgagaa cggcgccctc
     5521 gctccgtgga actccacgcg cctcgagtac tggaaccgga tgctggcctc ggtctgcgag
     5581 gcgcatggga tccccaccga cgttccgtgg cggaagctgg cgaaggagca gcggaagacg
     5641 ctgctgtacg gctccgacga cgaggtgcac atccgctacc gcaaccggta cgggcgcgtc
     5701 cgctcctact ggacgaccta cgagggcatc atccccaacc tcgagcggcg ccacgacgag
     5761 accgagtctg agttcagccg cgaccgcatc gagcagtaca tgcgggaggt gccctgccgg
     5821 acgtgcaggg gcgcccggct caaggccgag tcgctcgccg tcacggtggg ggagctcaac
     5881 atctcggagc tcacggcgaa gtcggtgggg aaagccctga cgttcctgct ggagctggag
     5941 gagaacctct cggaccggga gcggatgatc gccgagcgcc tgctccgcga gatccgatcg
     6001 cgcctgggct tcctggtgga cgtcgggctc gactacctga cgctggaccg agctgcggcc
     6061 acgctggccg ggggggaggc acagcggatc cgcctcgcca cccagatcgg ctcgggcctc
     6121 gtgggggtgt tgtacatcct ggacgagccc tcgatcggcc tgcatcagcg ggacaaccag
     6181 cggctcatcg acacgctggt ccggcttcgg aacctgggca acacgctcat cgtcgtggag
     6241 cacgacgagg ccacgatccg cacggccgac tggatcgtgg acatcggccc gggggccggg
     6301 gcggccggcg gcgagatcgt ctactcgggc gaggtcaagg gcctgttcga gcaccccggc
     6361 tcgctgaccg gcgagtacct ctcaggtgag cgcgagattc ccgtgcccag cgtccggcgg
     6421 tctgcctcgg gctggctcga ggtcaaggcc gccgcggagc acaacctgaa ggggatcgac
     6481 gtgcgcttcc ccttggggac gttcatctgc gtcaccggcg tctccggatc cgggaagtcc
     6541 accctcgtcg aggacgtcct ttcccgagcg ctccagcaga agattcaggg gtcgagggtc
     6601 gtccccggca agcaccgccg cctcgtggcc tgggagcgag tggacaagct cgtggtcgtg
     6661 gaccagtcgc cgatcggacg cacgccgcgg tccaaccctg ccacctacac cggggtcttc
     6721 gaccacatcc ggcggctcta cgcccaggtg cccgaggcgc gggtgcgggg gtaccagccg
     6781 ggccggttct cgttcaacgt gcggggaggc cgctgcgagg cctgctcggg cgagggccag
     6841 atcaagatcg agatgcactt cctgccggac gtctacgtga cgtgtgagca gtgcaaggga
     6901 cgccggtaca accgggagac gctggaggtc cgatacaagc atcggacgat cgccgacgtg
     6961 ctggacatga gcgtggacga ggctctggcc ttcttccaga accagcccac gatcgcccgc
     7021 tacctccaga cgctctccga cgtggggctg ggctacctgc ggctggggca gcccgccccc
     7081 acgctttcgg gcggggaggc gcagcgggtg aagctggcct ccgagctcca gcggagatcg
     7141 accggcagca cgttctacat cctcgacgag cccacgacgg gcctgcactt cgaggacgtg
     7201 cggaagctgc tgggcgtggt cgagcggctc gttgcggcgg gcaacacggt cgtcgtgatc
     7261 gagcacaacc tggacgtggt gaagacggcc gactggctga tcgacctggg gcccgaggga
     7321 ggagacgctg ggggcgagat cgtgaccgag ggcacccccg aggaggtcgc ggcggcgccc
     7381 gggtcgcaca cgggctcgtt cctggcccag gtcctcgggc gcgagcgcgc ggccgccgga
     7441 tgacccggcc tctgacaccg cggtcgggtt cgtgatacac aatcgctcgc gatgatccga
     7501 ggccgccatc cgctgctcgc cctcgtcgcc gtggtcgccg cttcctgcgg acccggcgac
     7561 gttcgcgtct cgagcgtccc catagaggag gtgggcttcg tcacgcagat cgtggacgtg
     7621 cagggcgagg cgggggccgg catctcgatc gcggcggacg ccgagggcag cccccacctc
     7681 gcgtacctgg cgttcgagcg cgagctggag gagggggagc agcccccgcc ccccgacccc
     7741 aacctgccgc tcttcccggc ggtccggcac gcgcatcaag cccagggcgt gtggagccgc
     7801 ggcactgcgg ccgaggagct cggcgacctg acgcagggcg acgggaccgc gatcgccgtg
     7861 gacggcgacg gcatggagca catcgcgtgg acggaggggg ggcggctccg gtactcgagc
     7921 aacggcgggg gcgcgttctc cgagcccgag accgtggtcc gagccgacga gctgcacggg
     7981 gtcgcgatcg cggcggccga ggacgggacg ccctcgatct cgttctacga cgtgccgggg
     8041 gggccggagg gtcccgcggc gctggtgcgc gtggccacgc cggccggggg aggctgggag
     8101 gtggagaccg cggccgaggc cgatcccaac cagccgatgt ccaccggcat cgggatcacc
     8161 cagaacggcg cgatcgtggc gtacggctcg gggccgacca cgtacgtcgc ccgcgacctc
     8221 gaggatcgct gggagtccga ggaggtcgac gccgggggcc tgggtgtgtc cctggacctc
     8281 gatgccgacg ggaatccgca cgtcagctac tacacgagtg acgggacggt gcgccacgcg
     8341 cactcggtcg gggggggtcc gtgggagacc tcggacgtcg gctcggcggg gcagggcccg
     8401 ggctccgact gggtcacctc gatctccgtg gatcccgagg gggtccactg ggtggcgtgg
     8461 cagaccgccg agggtctggg cctggcgtcg aacgaggagg gctcgttcca ggagcacgac
     8521 ctcccgggca cgcagggcgg agtgaggccg caggtggcga ccggggcgga gggcgccgtc
     8581 tacgtggcct ggcacgacac cgaggacggg acggccaatg tctcctcgcg cacctcggaa
     8641 gagccgctgc tcgccgggcc cccggagacc ggcgctcccc agcccgggcc cacaggcgat
     8701 ggcgggggag gcggaggcgg ggacggcggc ccgcccccct gcgagccgag cggcaccgag
     8761 ctggcgatcg tggcgccggt gggcgccgtg gccgacgggt tcgacaccga ctgcctggcc
     8821 gcccccgccg gcgaggcgtt cacgatcgac ttcgacaacc aggacaccgg cgtgccccac
     8881 aacgtctcga tctacgcgga cgacacggcg gcagagcggt tcttcgaggg tgagatcatc
     8941 acggggcccg accagatcac ctacgagccc gacccgatcg acgacgcggg ggtgttctac
     9001 ttccactgcg atgtgcaccc caacatgaac ggcgcgttcg tggtgcagta ggcccgccct
     9061 agaatgggcg cgtgaccacg ctccgccgcc ccgagacctc gacgatcccc gacgaccccg
     9121 gcgcctacct gttccgggac gcggacgggc gcgtgatcta tgcggggaag gccaagtccc
     9181 tgcggaagcg gctcgtgacc tactgggcga agccccagca cccccgcacg caggcgatgc
     9241 tcgacgcggc cgcggccgtg gagtggatcg tcgcctcagg agaggtcgac gccctcatgc
     9301 tcgagtacaa cctgatccag cggcaccggc cgcgcttcaa cgtccgctac cgggacgaca
     9361 agtcgtaccc gtacctcgcg gtcacggtgg gggagacctg gccccgagcg tacgtcatgc
     9421 gggggaagcg caggaagggc gtgcggcgct tcggtcccta cgggcacgcc tacgcgatcc
     9481 gcgacacgct cgactcgctg acgcgggtct tccccgtccg cacctgctcc aacgggttct
     9541 tcgaccagcg gcggcgggct cggcggccgt gcatctacta cgacatcggc cggtgcctcg
     9601 ggccatgcgt ccccgagcgg accggcgtca ccgaggaggg ctaccgggcc gaggtcgagg
     9661 ccctgatgga cttcctggac gggaactacc gtcccgtcct cgatcgcctc gacgaggaga
     9721 tgcggggcgc cgccgagcgc caggagttcg aggccgcggc caggttccgc gaccggatcg
     9781 ccgccgcgcg gagggcgatg gagcagcagg agatggtgct gggccggccc caggatctcg
     9841 acgtgatcgg gatggtcgag gacgacctcg aggccgggtt ccaggtgttc ttcgtgcgcc
     9901 gggggcgggt gatggggcgc cggggctggg tggccgatcg cgtcgaggac ctcgacacgc
     9961 cggccctcgt ctcctccctc gttcgcgagc tgtacatgga gcgggacgag gtccctccca
    10021 gggtcctggt ccctgcctgg ccgctcgatc gccaggtgct ggaacggtgg ctgggggagc
    10081 gccgcggcgg ccgcgtggag atcacggtgc cgcggcgggg ggagaagcgg cggctccagc
    10141 aggtggtgac cacgaacgcg aaggaggcgt tcgtccggca caagctccag cgggccaccg
    10201 acttcgcctc gcggtcgagg gctctcgacg agctggggcg ggcgctgggg ctcgaggacg
    10261 cgccgctccg gatcgaggcg tacgacatct cgaacctcgt tggcaccgag aaggtgggct
    10321 cgatggtggt cttcgaggac ggcttgccga agcgctccga atatcgacgg ttccggatca
    10381 agggggtgcc cggccaggac gacgtggcga gcatggagga gacgctgagg cgtcgcttcg
    10441 cccggtacct ggaggagcgc gagcggccgg cctccgagcg gaagcggttc gcctaccctc
    10501 cctcgctcgt ggtggtggac ggggggcgcg gccagctggg cgccgcggtg cgggtcctga
    10561 acgagctggg gctggagatc cccgcggcgg gcctggccaa gcgcctggag gaggtctacc
    10621 tgccggggaa gcccgacccg ctccggatcc cgcgaggctc ggaggccctg tacctgctcc
    10681 agcacgtccg ggacgaggcc catcggttcg ccctcgacct ccacaggaag cggcgcgagc
    10741 gccgggccct cgcctcccct ctcgatgagg tgcccgggat cgggcccgcg cggaagaagg
    10801 ccctcctgcg gcggttcggc tcgctgaagc ggctggcgaa ggcctcgccc gacgacatcg
    10861 cggaggtccc cggcgtggga ccggcgctcg cgcagcaggt ccacgaggcc ctcacccgga
    10921 ggtcggcgtg agcggccgcc gagcccgcgc cagggagcac cgcgccgcgc gggagcaggc
    10981 gagacagcgg gccctccaga ccgacttcac gatcatcacc ggtctgtcgg gcgcgggacg
    11041 ctccgaggcc gcgaagtgcc tcgaggacct gggctacttc gtggtggaca acctgccgcc
    11101 ggccctgatc ggcaagatgg ccgagctcgt ggcccagtcc gaggagccgc gccgcgtggc
    11161 gatcgtggcc gacgcgaggg gcggcgtgtt cttcgccgac ctggcgaagg gcctcgagga
    11221 gctccgcgaa gccggaaccc cggcccgcat cctctacctg gaggcggacg acgagacgct
    11281 ggtgatgcgc ttcgccgcga cccgcaggcg tcaccctctg gcccccgccg accggatggt
    11341 cgagggcatc cggaaggagc gggacctgat ggaggcgctc cgcgaggagg ctgatctcat
    11401 catcgacacg acggggctca cgcccaacgg gctccgggag cggatccggg acacgttcgc
    11461 cgctgcgcgt gccgaggacg ggctccaggt ggcggtggtg tcgttcggct acaagcacgg
    11521 cgtgccccgc gacgcggacc tcgttctcga ctgccggttc ctccccaacc cccactggat
    11581 cgaggagctg cggcccctgc cggggacgga cgaggcggtc cgctcctacg tgatcagcca
    11641 gcacgcctac ggagagttca tgaagcgcct ccgatccctg ctcgacttcg tcctgcccgg
    11701 ctacgtggcc gaggggaagt cgtacctgac gatcgcgatc ggctgcacgg gtgggcggca
    11761 ccggtcggtg gtcgtggccg aggagatcgc cggccacctc gagtcccagg agcgccgggt
    11821 ggccctcgag caccgggacc tggagcgcga gtgagggctc gcttcgggcc cggccccgtc
    11881 gggtactagg atgaggacca cccgcacagg aggctgagac acatgggcgt caaggtaggc
    11941 atcaacggct tcggcaggat cggccgcaac ttcttccggg cggcgaagca gcgcggcctc
    12001 gacctcgact tcgtcggggt gaacgacatc accgacgccg ccacgctggc ccacctgctg
    12061 aagtacgact cggtgctggg gaccctcgat gccgacatcc aggcgagcga ggggggcatc
    12121 accgtggacg gcgacgagat ccgggtcctc gccgagcgcg acccgggggc cctcccctgg
    12181 aaggacctgg gggcggaggt cgtcgtggag tccaccgggc tcttcacgaa gcgggaggac
    12241 gccgagaagc acctctcggc cggggcccag aaggtggtga tcagcgcccc cgcaaagggc
    12301 gaggacatca cgatcgtcct gggcgtgaac cacgaggctt acgacccgtc gagccaccac
    12361 gtcatctcca atgcgtcgtg caccacgaac tgcgtggtcc ccatggccaa ggtcctggcg
    12421 gacgccttcg ggatcgaccg cgcgctgatg acgacgatcc acgcctacac gaacgaccag
    12481 aacatcctcg atctgccgca caaggacctg cgacgggcgc gggcggcggc catcaacatc
    12541 atcccgacgt ccacgggcgc ggcgaaggcc acgtccctgg cgatccccga gctcaagggg
    12601 aagatggacg gcgtggcgat gcgtgttccc gtccccgacg gatcgctgac cgacctcgtc
    12661 tgcgtgctgg agcaggagcc ctcggtggac gaggtgaacg aggcgttcgc cgccgcgggt
    12721 tccggccccc tgcaggggat cctccgctac agcgaggatc cgatcgtctc cagcgacatc
    12781 gtgggcgatc cccactcgtg catcttcgac gcgccgtcca cgatggcgat cggcaacctg
    12841 gtgaaggtcc tcggctggta cgacaacgag tggggctatt cgaaccggct cgtggatctc
    12901 gtcgagatgg tcggctcctc gctctgacgg tggggaggct gcgagggatc gaggacctcg
    12961 gcgatccgtc ggggcgccgg gtgttcgtgc gagcggactt caacgtgccg ctggaaggcg
    13021 gccgggtcgc cgacgacctg cggatccggg ccacgcttcc cacgatcgag gagcttcggg
    13081 ggaggggcgc ggcgctcgtg ctggccagcc acctggggag gccgaagggc gaaccccggg
    13141 ccgagctccg gatggacccg gtggcgaggc ggctcgagga gctggcgggc gcgcccgtga
    13201 ccaaggtgga cgaggtcgtg gggcccgagg tcgagaaggc cgcccatgcg ctcgaccccg
    13261 gggggctcct cctcctggag aacctccgct tcgatcccgg cgaggaggcg aacgacccgg
    13321 gcttggcccg gcggctcgcc gggctggccg acgcgtacgt gaacgacgcc ttcggggcgg
    13381 cccaccgggc gcacgcctcg gtcgtgggga tcgccgagca tctcccctcg gccgccggcc
    13441 gcctcatggc ccgtgagatc gaggtgctgt cccgcctcct ggagggcccg gagcggccct
    13501 tcgtggcgat cctggggggg gccaaggtct cggacaagct gggggtcgtt cgctcgctca
    13561 tggagcgggt ggatgcgctc ctgatcggag gcgccatggc cttcacgttc attctggccc
    13621 agggcggaca ggtgggctcg tccatggtcg aggccgaacg cgtcggcgag gtccgcgaga
    13681 cgatggaggc ggcctcccgg cggggcgtgc tgatcgtcct ccccgaggac gtcgtggcgg
    13741 cggcccagcc ggcccaggac gcgcggaagc agacggtgcg cgcgggcgaa gtccccgccg
    13801 ggctcatggg gctcgacatc ggcccccgct cggtcgagga gttcgcccgc gtcctggccg
    13861 acgcccggac gatcttctgg aacgggccga tgggcgtggt ggagctggag ccgttctcgc
    13921 tcgggacccg cggggtggcc caggccgtgg ccggggccgg cgctttctcg gtcgtgggcg
    13981 gcggcgacag catcgcggcg atccggaggc tggggttcga ggacgacgtg gaccacctgt
    14041 ccacgggcgg cggcgcgtcg ctcgagttcc tggaggggcg ggagctcccc ggggtggccc
    14101 ccctgatgga ggagaacgat ggctgagcga cgcccgatca tcgcggcgaa ctggaagatg
    14161 cacaagacgc acctcgaggc gatcgagacg gtgcagaagc tctcgtatct gctggacgag
    14221 gacgacaccg agcgggtcga ggtcgtgatc tgcccgccct tcacggccct ccgcagcatc
    14281 cagacggtga tctccggcga tcgcctcccg ttcgggatcg gggcccagga cgtccactgg
    14341 gaggagaagg gcgcgctgac gggcgaggtg tcccccccga tgctggcggc gctggactgc
    14401 gcctatgtga tcgtgggcca ctccgagcgc aggcagcact tcggagaaac cgacgccgac
    14461 gtggcccgca aagcgcgggc ggcgctggcg gcggggatcg tccccatcgt gtgcgtgggc
    14521 gagacgctcg aggagcggga cgcggggcgg accgaggaga aggtgacgag gcaggtccgc
    14581 gaggcgttgg ccaaggttcc gcccgaccgg gccggcgagc tcgtgctcgc ctacgagccg
    14641 atctgggcca tcgggacggg ccgggccgcg acgccccagg acgccggcga agtgatcgca
    14701 gcgatccgcg ccacgctgag ctcgatgttc gcccacgagg tggcggaggc cgtccgcgtc
    14761 cagtacgggg gaagcgtgaa ggcgggcaac atccgggagt tcatggccca cccggaggtg
    14821 gacggggccc tggtgggcgg agccagcctc gaccccgagg aactcgcgct gatcgtgagg
    14881 taccgatgaa gaagctgttc tacgtgtgcc tcgacggcct gggggacgag cccctgccgg
    14941 ccctcgggga ccgtaccccc ctggaggccg cctccacccc gctcctcgac gacctggcgg
    15001 cgaggggcag gcaggggctc gtccacaccg tcggccccga cgtggccccc gagtccgaca
    15061 tcgccgtctt cgccatcctc ggctacgacc cccgcgagga gcaccccggg cggggtgtcc
    15121 tggaggccct cggctctggg atggccttcg aggacgggga cctcgcctat cgggtgaatt
    15181 tcgccaccgc cgagcacggc cgcatcgtgg accggcgcgt gggtcgcgac ctctcctccg
    15241 aggaggcctc tgccttggcg gatgaggtca accgagaggt cgagctcccc ggcgccgcct
    15301 ccgagctgcg tgccaccgtg gagcatcgcg gcgccctggt gatccgggcc tcagacggcc
    15361 cgctctcggc gcaggtctcg aacactgacc ccgcctacgc gaaggtcggc ccgcttggtg
    15421 tggccctgga gaccttcggg gaccgcccgc tgcccgccca gcccctcgac gacaccgagg
    15481 tggcccgccg gagcgccgag ctcacgaacg cctggaccga gcgggccggt gcgatcctgg
    15541 aggcctccga ggtgaacacg ggccggcggg ccggcggacg cctgcccgcc aacctcgtcc
    15601 tggctcggga cgccggcgat cacatccctc ggctcgtccc tatcaaggag cgcttcgggc
    15661 cgtcatgggg atgcttcgtc gagatgccgg tcgagcgggg catcgccatg atcctgggga
    15721 tgggggaggt gccggcgccc accccggcgg accccgccga gcggtacgcc gcctggggac
    15781 agatggccct ggaggcgatc ggcgggtacg acgcgctgta catccacatc aaggggccgg
    15841 acgtgccggc gcacgacggt cgggccgagg acaagcgaga cgtgatcgag acgatcgacg
    15901 ccgcgttctt cgggaccctc ctccccggca tcgaccccgg cggcacgatc ctggccgtca
    15961 ccgccgacca cgccacctcc tgcgcccgca aggcccacac ggcggacccc gtgccgctga
    16021 tcgtggccgg cggaggggtg gacccggacg gcacgaccgc cttcggcgag cgggccgccg
    16081 cggacggctc gatcgggacg atccccgggg tgcagatcgt cccgaccctc gtcgatctcc
    16141 tctgatcccc aatcctcgtc cgaggggagc gcgtatacct ccacgaaact gaccgtgccc
    16201 gatcgcaggc tcttgacctt tgctcagagg gctcgagccg ggtagtatcc ggcaggttgt
    16261 cctggaaggg gggccgtgtg tcgcaggtcg cgaccgaacc catcgacgag tctgggttgg
    16321 gcccgggacc gggtggggag cccggccaga tcgtcggacg gagcccgtgg gagctcttct
    16381 ggcgccggtt ccgccaggac cggctcgcca cggcgggcat catcatcatc gcgatcatcg
    16441 ccgccctggc gctgaccgcg ccgctcttct ccaggctcgt gggccacgat cccaaccaga
    16501 ccttcgccca ggagtacccg gcgggggctc tcgacgactt cggcctgccg atcggcccca
    16561 acaaggactt ctggttcggg gccgacaccg cgggccgcga cctcttcgtg agggttctct
    16621 acggcgcgca gacctcgctc tccgtcgcgc tgctggcgac cgcgatctcc ctggtgatcg
    16681 gggtcgtggg cggcctcttc gccggcttct accggggcaa ggtcgacacg acggtctcgc
    16741 gcctgatcga catcgtgctg gccatgccgg tgctgctcct ggcgctgggg ctcgtggcgg
    16801 cgtgcggcac ggcggagggc tgtctgggcg gcctgtgggg cttccttaaa ccggggtgga
    16861 ggctcgtggc cctcatcatc ggcttgttca gctggcccta catcgctcgg atcgtccgcg
    16921 gccaggtcct ctccctgcgg gagaaggagt tcgtcgaggc ggcgaggtcc atgggctcga
    16981 gcgatcgacg catcatcttc cgggaggtgc tgccgaacgt cgtggccccc atcatcgtct
    17041 acaccacgct gatcatcccg aacaacatcc tgttcgaggc cgcgctctcg ttcctcggcg
    17101 tgggcgtgcc tccgacgact ccgtcttggg gacgcatgct cgccgaaggc ggctccaact
    17161 tcacgttcgc ctggtggatg atggtgttcc ccggcgtgtt cctgttcctg acgacgctcg
    17221 cgttcaacct cgtcggggac gggctgcggg acgctctcga ccccaagatg tcgagcgtga
    17281 ggaggaagaa gagcaagcga caggctgccc aggaggcgag cgccgaggcc gaggcctccc
    17341 tggcgggatg aaggaaggag gagcgctgag cggacgaacg agcagtgaag ggtacgaaga
    17401 gagaggggga cgatgtgagt aggaagcacc tcaagctcat cgccctcgcc ggagtggtcg
    17461 cgctggccgc cgcggcgtgt ggcggcgggg acaccggcgg gaccggcgag gatgaccaac
    17521 aggaagagga ggtccagaag ggagggacgc ttcagctgaa cctcctgtcg gacgtgatct
    17581 ccgctttcga tccgcagaag gagtactact cggtgacgtg ggagttctac cggtgctgtc
    17641 tgctccggac gctcatgtcc tacaacggca tgtcgaccga ggagggcggg accgagatcc
    17701 agccggacct ggcgacggcg atgccggagg tctccgatga cgggctcacc tggaccttct
    17761 cgatccgtga gggcgtgaac tacgcaccgc ccttcgacga caccgagatc gtggcccagg
    17821 acttcgtccg ggcgctggag cgtctggcgg atcccgacgc ctcggcgggg ggctatgcct
    17881 tctactactc ggtcatcgag gggttcgacg acttctcggc cggggaggcg gacagcatct
    17941 ccgggctgaa cgtcgtcgat gaccacaccc tcgaggtcac gctgaccgac cccgcgggcg
    18001 acttcgggta ccggatggcc atgccggcca cggccccgat ccccgagggc gcggccgacg
    18061 gccacgtcca ggactacggc cggttcctcg tggcctcggg cccctacatg ttcgagggca
    18121 gcgagaacct ggacttctcg gcggctccga accagcagga gccggtctcg gggtacgtcc
    18181 ccggccagtc gatcaccttc gttcggaacc cgtcgtggtc gaacgagatc gacgacctgc
    18241 gccccgccaa cgtggatcgc atcgagatca cgatcggcgg caccgaggag gacaacgcca
    18301 acaaggtgga ctcgggcgag ctcgacatgc agttcgacgg cgtggcgccc cccgagcagg
    18361 tccgcaagta ccagaccgac ccgcagctcg aggatcagct cttcgtgaac ccctcggacg
    18421 cggtgcggta cctgaccatc aacctggcga tgccgccgtt cgacgacatc cacgtccgga
    18481 aggccatcaa cctggccctc gacaaggact cgatgcgccg gatccgcggc ggtcctctgg
    18541 tgggggagct cgccgggcac atcatggtcg acagcctcca gaacaacctg ctggtggact
    18601 acgacccgta cgagacgccg aacgggcagg gggacatcga ggccgcgcag gccgagatgg
    18661 cccagtcgca gtacgacacg gacggggacg gggtctgcga cgacccggtg tgcgagaaca
    18721 tccccacgaa cacggatgag gccgatccct acccggacca ggccgcgctg atccagcaga
    18781 acttcgagcc cctggggctc acgctggaca tccggcagtt cgagcggggc acgatgtacg
    18841 accgggtgaa cgaccccaac acgcagaccg cgctggggct cgcgcccggg tggggcaagg
    18901 actacctcga cgggaccacc ttcggggagc ccctgttcgg cgcggcctcg atcggtccgg
    18961 acgcctgctg caactactcg ctcgtcggcg cgaccgcgga gcagctggag ggcttcggat
    19021 acgacgtgac cgaggccccg agcgtcgacg acaagatcga cgagtgcgag gcggccaccg
    19081 acgacgcgcg gttccagtgc tgggccgagc tcgaccagga gctcatggag aacgtcgtgc
    19141 cgtgggtgcc gtacctgttc gacaactacg tgcggacggt gtcggagcgt gtggagaact
    19201 acaccttcga ccagttcgcc gggctgccgg ccctcgacca catcgccctc gcgggtggtg
    19261 gcgccgagag ctaggctcga tggagcgcga ggggccgccc tccggggcgg cccctcgctc
    19321 catgcgatgg ctgcaggggg ggtgacgtgg gccgatacct gatccgtcgg acgctgttcc
    19381 tcgtcctcgt cctgttcatc atctcggtcc tcaccttcct gatcttcgtc aagctcccgc
    19441 ccggtgatcc cgcacgccgg ttcgccggcc ggcgtgccac cgcccagcag atccagcagg
    19501 tgcgggagca gcttggcctg aaccgatcgg tgttcgtgca gtactggtcc ctggccaagg
    19561 ggctgatccc atggcccccg cagtccgtcc tcaccgccga ccccgacgcg gggctcttcc
    19621 tgaaccgcga cagctactac tcctacgtga acttcgtgcc ggtgaaggag gagatctacc
    19681 gccggctccc cgtgaccatc acgctggcgc tgggcggggc cgtggtctgg gtgctgatcg
    19741 gtcttcccgt cggcatcgtg tcggccgtgc gtccccggtc gctcgtggac cgctccggga
    19801 tgatcttcgc cctcttcggg gtgtccgccc cggtcttctg gctgggcttc gtgttcctct
    19861 acatcttctg gttccggtta gggatcctgc ccgggagcgg gctggagcag ggccagaccc
    19921 tcgtggagaa cgtgctggcc gggcggttca tcatgccctg ggtggtgatc tcactctcct
    19981 tcgccgcgtt ctacgcccgg atggtccgcg ggaacctcat cgagacgatg ggggaggact
    20041 acatccggac ggcgcgggcc aaggggctct ccgagcgaag ggtgatcctt cgccacgggc
    20101 tccgctccgc gctcaccccc gtcgtcacga tgttcggcat ggacatgggc atcctgctgg
    20161 gcggggccat catcacagaa accgttttca atctcccggg gatcgggcag tacgccatcc
    20221 gctccctcct gaccaacgac atccccgcgg tcatgggcgt gacggtcctc gccgcgttct
    20281 tcatcgtggt ggccaacctc gtcgtggaca tcgtgtacgc cttcctagac ccccgcgtga
    20341 ggtatacgta gtctccgtga cggacgatcg agagagggga acgggtggct gagccgctgc
    20401 tcgaggtgaa ggatctcagg gtccacttcc cgaccgagga cgggctggtg aaggccgtgg
    20461 acggcgtctc gttcacggtg cagccggggg agacgctcgg cgtggtgggg gagtccggct
    20521 cggggaagag cgttacgttc ctgacggtcc tgggccttgt cagccgccgg gaggccgaga
    20581 tctcggggca gatcctcttc cgggggaagg atctcctcca gacccctgag gatgagctcc
    20641 gcgagttcag ggggagccag gccagcatga tcttccagga ccccatgacc tcgctccacc
    20701 cctactacaa agtggggaag cagatcgtcg aggccatcct cacgcacgag aagatctcga
    20761 agcgggaggc catggaccag tccgtcgaga tgctccgccg cgtgggcatc ccccgccccg
    20821 aggagcgggc gcgccagtat ccgcacgagt actcgggagg gatgcgccag cgagccatga
    20881 tcgccatggc gctcgcgctc aaccccgacc tcctgatcgc cgacgagccc acgaccgcgc
    20941 tcgacgtgac cgtgcaggcg cagatcctcg acctgatcga ccgcctcaag gaggagttcg
    21001 gcgcggccgt cgtgatcatc acgcacgacc tcggggtcgt cgccgagcac tgcgacaaca
    21061 tcatggtgat gtacgcaggc aagcccgccg agtacgggac gcgccaggac atctactacg
    21121 acgcgcacca cccctacacg tgggggctgc tgggatcgat cacccgcctc gaccagctcc
    21181 gcagcgaccg cctccggccc atccccggtc tcccgccctc gctgatccag gtcccgccgg
    21241 gctgcccgtt ccacccgagg tgcccctacg tcatggacgt ctgcaaggtc gaggtgcccc
    21301 agctactcgc cgacgactcg ggctcgcacc gctcggcctg ccacctgccg ctcacggaga
    21361 aggagcgcat attccaggag gaggtggccc tcacgtgacc ggggcgatcc ccgcgccgca
    21421 ggctcccatg gcccccgagc ccgactcctc ccggacgccg ctggtcaagg tggtcggcct
    21481 gaagaagcac ttcccgatca cgcgtggcgt cctcttccag cgaaaggtgg gcgccgtcca
    21541 cgcggtggac gggatcgacc tcgaggtcta tcccggcgag acggtcggcc tggtgggcga
    21601 gaccggctgc ggcaagtcca ccacggcccg gctcgtcatg aagctcctcg aggccaccga
    21661 gggacaggtc ttctacgacg ggcaggacat caccaggtac gggcgccgcc ggatgcggga
    21721 gctccgcggc gagatgcaga tgatcttcca ggaccccttc gcctcgctca acccccggaa
    21781 gaccgtgggc tcgatcatcg gcgagccgtt ccgggtccac ggcaccacgc ccaaggccgg
    21841 gatcaagcgc gaagtgcaga tcctgatgga gctcgtcggc ctcaaccccg agcactacaa
    21901 ccgctacccg cacgagttct cgggcggcca gcgccagcgg atcggcgtgg cccgcgccct
    21961 cgctctccgg ccgcgtctga tcgtggcgga cgagcccgtc tcggccctcg acgtctcgat
    22021 ccaggctcag atcctcaacc tcctggagga cctccaggag gagttcaacc tcacctacct
    22081 cttcatcgcc cacgacctct cggttgtgaa gcacatgagc gacagggtcg cggtgatgta
    22141 cctggggaag atcgtcgaga tcgcgccggg cgagaccctg taccgcaacc ccaagcaccc
    22201 gtacacgggc gcgctgctct cggcgatccc gatcccggac ccgaaggcgg cggagcagag
    22261 gcgacgcatc gtgatcgagg gggacgtgcc gtccccgatc gaccccccgt cggggtgccg
    22321 gttccatcct cggtgcccga aggcgcagtt cccgacctgt tcggacatcg agccactcct
    22381 cgagc
//
LOCUS       DASUTZ010000016        20359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_651662, whole genome shotgun sequence.
ACCESSION   DASUTZ010000016 DASUTZ010000000
VERSION     DASUTZ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 20359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20359
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651662"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VF097_00650"
     CDS             complement(<1..849)
                     /locus_tag="VF097_00650"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX6261333.1"
                     /translation="MGAATRDAFGETIVRVGEDPRIVVLTGDLKDSTRTEAFEEAYPE
                     RFIECGIAERNMIGVAAGLALSGKIPWAASFSTFVAGRFETVRVSVAFQEANVRIVGT
                     HVGIGVGEDGYSQMAFEDVALMRTLPNMTIIQPADAAETHAAVEYLVEYEGPAFLRLT
                     RQGVDDVHGEGYRFEPGRIERLRDGDDVALIASGATVAEALRSADLLSEDGISSRVLN
                     VHTIKPLDEEAVLEAAADCRALITVEDGSAVGGLGGAVAETVATQGGQAPLRIIGHRT
                     FGESGSP"
     gene            complement(849..1691)
                     /locus_tag="VF097_00655"
     CDS             complement(849..1691)
                     /locus_tag="VF097_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX6261334.1"
                     /translation="MEADTREDLIARLEEAARDLRITDIEMLAEAGSGHPGGTLSAAE
                     MVAALFFHKLRVRPEEPEWPDRDRFVLSKGHCIPIVYAAMAKLGFFEEKELMTLRKLG
                     TRLQGHPDRRLLPGIEASTGSLGQGLSTAAGMALAAKLDGADWRVYCMMGDGEIQAGQ
                     MWEAAMFAPRHGIDTLTAILDHNQVQQTGKVAETMPIEPIVAKWTAFNWHVREIDGHD
                     MAQVLDALDEAEMVTGKPTIIVSHTVKGKGVSWMELDPSWHGKAPSAEEAERAIAELR
                     GEGS"
     gene            1752..3167
                     /gene="nhaA"
                     /locus_tag="VF097_00660"
     CDS             1752..3167
                     /gene="nhaA"
                     /locus_tag="VF097_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEX6261335.1"
                     /translation="MTDRTDASTDHGTDEGTERLRVPWSRSDRRLPRLVVRPLQSFLD
                     TEEAGGVLLLLAAGAALLWANLWPGSYESVWHTDLILRLGSWELSDDLQHWINDGLMA
                     LFFFVVGLEIKRELVVGELRDPKTAAFPTVAALGGMIVPAAFYLALNAGGPGVHGWGI
                     PMATDIAFAVGVLALLGRGLPSSLRVFLLSLAIVDDIGAILVIAIFYSSGISWAWLAL
                     AAGFLVLIAAAQRVYVRFTAVYLILGVGTWLAVYLSGVHATIAGVALGLLTPAMPFQM
                     PGPVSERARRVADRTADTVEHADEDAHHWLGLARLSREAVSPLARIEHALHPWTSYVI
                     IPLFALANAGVELGGGAIGEAATSRVTLGVILGLVAGKILGITLFSWGAVRLGLARLP
                     SGSGWTQVTAVSAVAGIGFTVSLFIASLAFTDPEILAAAKVGILAASIVAGAIGAALL
                     AMSRRRRAADREPVAEGGAAV"
     gene            complement(3164..4274)
                     /gene="prfB"
                     /locus_tag="VF097_00665"
     CDS             complement(join(3164..4207,4209..4274))
                     /gene="prfB"
                     /locus_tag="VF097_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153759895.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX6261336.1"
                     /translation="MSEHAERIRGLSERLSSAKEYLDLHAKRAEMAELEQLLSDPSLW
                     DDHARAQQLTTRLARLRDDVERYERLAERLEEAEVLDQLLDEQADPDTERELKEHLEA
                     LASDVDRLELAVLLSGEFDDADAIATLHAGAGGTESQDWAEMLLRMYLRWAERQGFEV
                     ELDEVNPGEEAGIKSATFTVKGRNAYGTLSAERGVHRLVRISPYDASSRRHTSFASLD
                     VVPVIEEDAEIEIDPNDLRIDTYRSSGAGGQHVNVTDSAVRITHLPTGITAACQNERS
                     QMQNRAVAMRILKARLAELARREREERIENLRGERREIQFGSQIRSYVLHPYRLVKDH
                     RTNLEVGNVDAVLDGDLDRLIEVELRRRAADGGEG"
     gene            4364..4705
                     /locus_tag="VF097_00670"
     CDS             4364..4705
                     /locus_tag="VF097_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261337.1"
                     /translation="MRRKSRSAARDRLHDALEALQRDGEPIILRAGRRPVGVLITVDD
                     YRKRFPGSGDGSASNGDGGTWPQVERRKGDRRRGERRGRRRDPAPREGDPGATLDVLR
                     ELRSLGITAER"
     gene            complement(4721..7384)
                     /gene="secA"
                     /locus_tag="VF097_00675"
     CDS             complement(4721..7384)
                     /gene="secA"
                     /locus_tag="VF097_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX6261338.1"
                     /translation="MVFSKVQQIGEKREHKRLSHWVELVSSYEPEMEDLTDDQLRGKT
                     DEFRARLKEGETLDDLLPEAFAAVREAARRTIGQRHYDVQVMGATVLHQGKIAEMKTG
                     EGKTLVSTMPIYLNALAGDGVHVVTVNDYLAKRDSEWMGPIYRALGISVGLIQSQMRP
                     EERRPNYAADVTYGTNNEFGFDYLRDNMAMRREDMVQRGHHFAIIDEVDSILIDEART
                     PLIISGPVQDSAKWYQTFARIAPRLKRDQDYEIDETKRTVAVSEDGVARVEDYLGIEN
                     LYEHVNTPLVHHLQNAIRAKELYKRDVDYIVQDGEVLIVDEFTGRVLHGRRYSEGLHQ
                     AIEAKEGVRIKEENQTLATITIQNYFRMYEKLGGMTGTAKTQAAEFEQVYKLPVVTIP
                     TNRDMIRRDEDDLIFKTEDAKWNALIEDIVERNGEGQPILVGTVSIEKSEHVSKLLQR
                     RGVTHNVLNAKQHEKEAMIIAQAGQKGAVTVATNMAGRGVDIMLGGNPEYLARQDMAA
                     KGWDNDRYLLFEMDPQERKAYEEEYGPLYERHKQTTDAEHEVVVDLGGLYVLGTERHE
                     SRRIDNQLRGRSGRQGDPGESRFYLSLGDDLMRLFASDRIGSIMNRLNWPEDEPITAR
                     MVTRAIESAQRQVEGVNFERRKNVLKYDEVMNTQRKIVYADRRRILEGESLQDRALDM
                     VGEVIQGVVAEYVSPEVFAEDWDLEGLLTQIHLTYPSDLTIADLKPIEDPLELSEKVL
                     QEALEAYEKREAELGEETMRELERMVFLNIIDNLWREHLQEMDYLQEGIGLRAYGQRD
                     PLVEYQNEAHAMFEAMKASMVEEFVRYIYRVEVVREDEPSRPRPQRMVAQHGDRQEAA
                     SPGAGTSGKVPRNAPCPCGSGKKYKKCHGAA"
     gene            complement(7417..8667)
                     /locus_tag="VF097_00680"
     CDS             complement(7417..8667)
                     /locus_tag="VF097_00680"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6261339.1"
                     /translation="MAVVRDDRVGAVRRATLLLAGAQMALWAGNGAYAAFGPLSIEEL
                     TGREGTAAQLFGVYALAAAASALVTGRVMDRLGRKPGLLGGYVLNAVAGLLAFAAVRA
                     GSPPLLYAAGGLLGAGTAAALLGRAAVADMYPPERRGRAVGLLLVAGTFGAIGGPLLA
                     GAIHTLASSAGVADPLAFPWLLVPVVALGAAGLVAAIRPDPRELAAPGPVPTAAPRSP
                     REILRLRPAATAVVAIGIAHAVMVTFMAQLPSSIHRHGASEVTVSIVVSLHLAGMFAF
                     SPAFGALIDRWGRRAGLLVGAALSASGVLLGVVFTRPIGAGTGLVLIGMGWSASYLGS
                     TAAVSDMATPWERGSALGVTDLVASLASGVGAIAGASLLRAAGLPALGGAGLALLGLV
                     VVLLVPLREPSPGRWEPRVASPTV"
     gene            complement(8699..9391)
                     /locus_tag="VF097_00685"
     CDS             complement(8699..9391)
                     /locus_tag="VF097_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6261340.1"
                     /translation="MSKKAAEKADGDALKVLIVDDHALFRRGIQMVLEQEEGIEVVGE
                     ATDGQEAVEKAEELMPDVVLMDVRMPKRTGIEATSAIKEAIPHAKILMLTVSDEEEDL
                     YEAIKAGASGYLLKEISTEDVADAIRSVWTGQSRISPAMASKLLNEFQAMSKRAEERE
                     SVHTPRLTDREMEVLRLVAKGMNNRDIAKELFISENTVKNHVRNILEKLQLHSRMEAV
                     VYAVREKLLEIK"
     gene            complement(9372..9752)
                     /locus_tag="VF097_00690"
     CDS             complement(9372..9752)
                     /locus_tag="VF097_00690"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEX6261341.1"
                     /translation="MDVVVKATKGRVPARARQVVERKAARLARHDWRVRRVEVNVIQE
                     ANPRVSAGHRVEGSAWTDRRSFHASGAGETLEVAVEQVVSRLDRQLAGHAGKRRRKLI
                     EGANRVKSRQVPEPPEVMDVEEGR"
     gene            complement(10040..10744)
                     /locus_tag="VF097_00695"
     CDS             complement(10040..10744)
                     /locus_tag="VF097_00695"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HEX6261342.1"
                     /translation="MLDAIFPPRCLGCSRRRVLLCERCVEDVGVIVPPLCERCGRPWA
                     APVEVCADCPPGGIDLARSPFLYEGPIAAAIRGMKFGGWRALAPRLAAAIGAVLPETE
                     TDAVCWVPLSARRRRRRGFDQAEVLGRRVARLLGLPAIAALRRRRDTAAQARLGGAER
                     RAALVGAFRARRRVPRSVLLVDDVLTTGATASACAAALREGGTERVVVATAARSVRGP
                     FPAGTFGGSRGAAPRL"
     gene            complement(10780..11487)
                     /locus_tag="VF097_00700"
     CDS             complement(10780..11487)
                     /locus_tag="VF097_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261343.1"
                     /translation="MGDGDLSGVSVDAVFERFPASVRGAVVVRGVDPDPHQVRLTEAV
                     VVEVGPSRAVRNLELGQVTVDVAPRDRVMIPFEVPFSDLEPGWYTVQAEVDVDGQESE
                     RGPREAERRFCVAWPSEAVRRGSVEAGIRIKVPGSRGAAIDRLVFRADSAAVHWRHAA
                     AEKPGFREFGDLKVTAGRRRLHVLDDEYEWSTGSRTTTIYPVMRGDEELTLELDRRHR
                     QGKPIQRGPWSATLSLP"
     gene            complement(11469..12683)
                     /gene="ahcY"
                     /locus_tag="VF097_00705"
     CDS             complement(11469..12683)
                     /gene="ahcY"
                     /locus_tag="VF097_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX6261344.1"
                     /translation="MGRERIEWAGREMRVLGLIGERFEKERPLDGLRVAACLHITTET
                     ANLLRTLRGGGAEVAACASNPLSTQDDVAAALCDEGIATFAIRGEDNDAYYAHIRAVL
                     DTRPHVTMDDGADLVNVLHEERAGQAGEVRAGTEETTTGVIRLRAMAKAGALRYPIVA
                     VNDAMTKHLFDNRFGTGQSTIDAIMRATNFLLAGRTVVVCGYGMCGRGVASRARGMGA
                     QIVVTEVSPLAALEAAMEGYRVMPMREAARVGDVFVTVTGDTSVIREEHLQAMKDGAV
                     LANAGHFDVEIDKEALSRLGGEPRRLRGSVDEYTMPDGRRIALLAEGRLVNLGAAEGH
                     PAAVMDMSFANQALVVEWLAGREGLEPEVLTVPDEIDTEVAALKLRAMGIEIDELTPE
                     QERYLGSWEMGT"
     gene            complement(12720..13544)
                     /locus_tag="VF097_00710"
     CDS             complement(12720..13544)
                     /locus_tag="VF097_00710"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX6261345.1"
                     /translation="MTVGPGDDLAERGAEAVRAVTDLRPQVAVIAGSGLGDALDAIEP
                     DAELAFSDLPGFPLPSVPGHAGRLVVGTLAEVPVAGFLGRVHFYEGHPMPLVTLPARL
                     AAALGAGTLVVTASTGGLDPDLEPGSLVIASDHLNLLGENPLRGWRDEEGRPVFVDLT
                     RVYPPGLAEAAERAAREVGLPFARGVYAAMPGPTYETPSEAEMLRRLGGDVVGMSVVP
                     EACAAAALGMRCLGLFCVTNRVGVHVSHEEVTRVAARFADGLRELLRRVLPALGEE"
     gene            complement(13541..14590)
                     /locus_tag="VF097_00715"
     CDS             complement(13541..14590)
                     /locus_tag="VF097_00715"
                     /inference="COORDINATES: protein motif:HMM:NF021916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HEX6261346.1"
                     /translation="MRSPIDASAIAAGDPSGMLEAFLRLPEQLVEGYEAGRAAGLSSV
                     EPRSLIICGMGGSAAGGDVVASAFADRLRLPVASVRGYSVPAWCGPGDLVMCLSFSGE
                     TEETVACLEEARRRGSTVTAVASGGRLIELAGEGAVTIPAAAPMPRAGLGLLAGGALG
                     ALVAMGLLPQADAEVKETAETLAELAGELAPGRDENEAMDVARAVGSQHVPVIWGSDG
                     LAEPAAFRWKAAFHENAKIPALSSALPELDHHEVAGWSKGRGHGFLVIALRHPGEHRT
                     VGPRLEATFEAIEESGIGWREAHARGRGPLAHSLSLILLGDVASVYHALARGVDPGPI
                     EAIDRLKRRLAGGRG"
     gene            complement(14595..14777)
                     /locus_tag="VF097_00720"
     CDS             complement(14595..14777)
                     /locus_tag="VF097_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015775384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX6261347.1"
                     /translation="MALDQELLDVLACPNCRGQVEYREEEGFIVCLGKCGYKYPVRDD
                     IPVMLIEEAVKPSGGG"
     gene            complement(14779..16113)
                     /gene="manB"
                     /locus_tag="VF097_00725"
     CDS             complement(14779..16113)
                     /gene="manB"
                     /locus_tag="VF097_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222900.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX6261348.1"
                     /translation="MAPPEIFKAYDIRGIYPDQLDEDTSRRIGAAFTEFTAAERVVVG
                     RDCRISSPDLSGAFIEGVVGRGADVVDIGLATTDMLYFASGSLDLPGAMFTASHNPPE
                     YNGLKLCREGAAPIGEETGLREIRDLADRDLAPSGAPGSVAERSMLDAYVDHVVGFVD
                     VSRLTPLTVVADAANGMAGLVLEPIFERLPAKLIGLYLDLDGTFPNHPADPIQPENQE
                     DLRKAVVEHGADVGLAFDGDADRVFVVDEGANGVSGSLLVALIADKMLEREPGATILY
                     NVICSWVVPEVIRASGGTPVRTRVGHSFIKQVMAETGAIFGGEHSGHYYFRDHYRADS
                     GLVAALLVLEQMSEAGLPLSELLRPFRRYHASGEINSEVEDQDAAIDRLAQRYTDGEQ
                     DRLDGLTVEYEDWWFNARPSNTEPLLRLNVEARDEGLLAERTAELLANIRGE"
     gene            complement(16152..16361)
                     /locus_tag="VF097_00730"
     CDS             complement(16152..16361)
                     /locus_tag="VF097_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261349.1"
                     /translation="MSAIKSKAHAAAPLTTCPGCGSSEVVVVTFGVGDSPIVFRTCPP
                     CEAKWWEQDGDLIDRETAIPLVTAA"
     gene            complement(16503..16793)
                     /locus_tag="VF097_00735"
     CDS             complement(16503..16793)
                     /locus_tag="VF097_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006885937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261350.1"
                     /translation="MLDHDLIRRIRALDEYDLRRLMIFVRGLLVARRNDDFEMEEPAR
                     AGKVTYRQELVRCGRESCGRCPHGPYWYAYWREDGRLRSRYIGKELPEDRPR"
     gene            complement(16827..17057)
                     /locus_tag="VF097_00740"
     CDS             complement(16827..17057)
                     /locus_tag="VF097_00740"
                     /inference="COORDINATES: protein motif:HMM:NF023430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 family protein"
                     /protein_id="HEX6261351.1"
                     /translation="MRRCAKMRCEEVAAASVAVRYSDRTAIVEPLSAERDPNLLDLCE
                     PHASNLTAPRGWRLVDHRPGAPELPSPVHASA"
     gene            complement(17330..18391)
                     /locus_tag="VF097_00745"
     CDS             complement(17330..18391)
                     /locus_tag="VF097_00745"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HEX6261352.1"
                     /translation="MRGRRHAAAGRRLLRVVVFTLVGLMVLGGGVAYGAYRFDRAQTG
                     RILPGVTIAGVDVGEMTRAEAIAAVGEAIESDLSQEIEVRARGRSWHVTPAELGTRGD
                     IEEKVDRALAVGEGMAWHERAFARIFDRPVQEDLRVRLRRDGEMIKRFLGIVSEATYV
                     SPNDASFGIEEGELVLTRPKMGRELDVKRSRERVRSALRQGKPLVTLPVSKVEPDVTA
                     DGLGLSIVVRISENRLYLYDGFKVIKRYDVATGTGGYPTPQGTWNIWDKRENPVWVNP
                     APDGWGADLPAVIGPGPGNPLGTHALYLDAPGIRIHGTYAEDTIGTFASHGCIRMRIE
                     ESKELFGMIPIGTKVHILP"
     gene            complement(18434..19795)
                     /locus_tag="VF097_00750"
     CDS             complement(18434..19795)
                     /locus_tag="VF097_00750"
                     /inference="COORDINATES: protein motif:HMM:NF040160.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5719 family protein"
                     /protein_id="HEX6261353.1"
                     /translation="MSRPWVERLRLPGALLVSLLVVAGGLALERGLGARTLTPGEVPS
                     AVSGAWFCPHGGSGRWRVWLVVANPTQEPAQIRATTYGGERPESQVATVGPGTQRYVE
                     VPASDPRAASAVEFFGGPVAAGMVIMRSTGRGEPLGRAAEPCLAESATRWYLPEGTTL
                     RGFDQRVVVMNPFPQEAVVSLWLTDERETIKPGDLQGIVLPGRRSASFDLGAFALGKE
                     TLAVTVDVGLGKVAVGGVGLSETGIRASTGVTASAGMWVMPGALDDEPSALTVLGTSR
                     AEAPFRARVQGTEGQLEVISEAVVPPGKAETHVVPAQGAGLVAVADGSELFVAGRRLN
                     TPGDLAGVPAIVEGAASWVALPTTAPAGGPSTLFLQNTGEEVADVRIVLVTATGPAEA
                     PEIGRIALRPGGYRLVDLSQFVGDEPVSVVVRAESGTVIAGQASRDPDGYAVASGAPL
                     DLP"
     gene            complement(19792..>20359)
                     /locus_tag="VF097_00755"
     CDS             complement(19792..>20359)
                     /locus_tag="VF097_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261354.1"
                     /translation="TMLAGRIRHGGSLLAPFAVAYVVAGEGDLPPEARLRLDAQLDLL
                     PGARAGGLVLYRNTRALPLAGILPAAPPDGDVRAVDALAPARVPRLGMAPFRRVEPNT
                     WTGAPDLEETGTAFVSKEYEPAWVLRAEDGRQYEPFRAFGWAIGVEAPPRSGRLTLDR
                     REGWVRTAEVAGLLVVWVGALWILRRRG"
BASE COUNT         2844 a         7572 c         6924 g         3019 t
ORIGIN      
        1 gggtgagccc gactcgccga acgtccggtg accgattatg cggagggggg cttggccgcc
       61 ctgggtggcc accgtctcgg ccaccgcgcc tcccaggccg ccgaccgcgc tgccgtcctc
      121 gaccgtgatg agcgcccggc agtccgcggc ggcttccagc acggcctcct cgtccagggg
      181 cttgatcgtg tgcacgttga ggacccgaga cgagatcccg tcctcggaca ggaggtcggc
      241 ggaccggaga gcctcggcca cggtggcgcc gctggcgatc agcgcgacgt cgtcgccgtc
      301 ccgcagccgc tcgatccgcc cgggctcgaa ccggtagccc tccccgtgga cgtcgtccac
      361 gccctgtcgg gtcagacgga ggaacgccgg gccctcgtac tcaacgaggt actcgacggc
      421 ggcgtgggtc tctgcggcgt cggccggctg gatgatcgtc atgttcggca gggtccgcat
      481 cagcgcgacg tcctcgaagg ccatctgcga gtagccgtcc tcgcccacgc cgatcccgac
      541 gtgggtgccc acgattcgca cgttcgcctc ctggaaggcc acggacaccc gcacggtctc
      601 gaaccggccg gccacgaagg tggagaacga ggccgcccac gggatcttcc ccgagagcgc
      661 gaggccggcc gcgaccccga tcatgttccg ctcggcgatg ccgcactcga tgaaccgctc
      721 ggggtaggcc tcctcgaagg cttcggttcg cgtggagtcc ttgaggtctc cggtcagcac
      781 gacgatgcga gggtcctcgc ccacgcggac gatcgtctcg ccgaaggcgt ccctggtggc
      841 cgcccccatc aggatccctc cccccgcagc tcggcgatcg cccgctcggc ctcctcggcg
      901 ctcggagcct tcccgtgcca ggaggggtcc agctccatcc aggagacccc cttgcccttc
      961 acagtgtgcg agacgatgat cgtcggcttc cccgtcacca tctcggcctc gtccagcgcg
     1021 tcgaggacct gggccatgtc gtgcccgtcg atctcccgga cgtgccagtt gaacgcggtc
     1081 cacttagcca cgatcggctc gatcggcatc gtctcggcca ccttccccgt ctgctggacc
     1141 tggttgtggt cgaggatcgc ggtgagggtg tcgatcccgt gccggggggc gaacatcgcc
     1201 gcctcccaca tctggccggc ctggatctca ccgtcgccca tcatgcagta aacccgccag
     1261 tcggccccgt cgagcttcgc ggccagcgcc atcccagcgg cggtggagag cccctggccc
     1321 agcgaccccg tggaggcctc gatccccggg aggagccggc ggtcggggtg gccctggagg
     1381 cgcgtgccga gcttgcggag cgtcatcagc tccttctcct cgaagaaccc cagcttggcc
     1441 atggccgcgt agacgatcgg gatgcagtgc cccttcgaca gcacgaagcg gtcgcggtcg
     1501 ggccactcgg gctcctcggg gcggacgcgt agcttgtgga agaacagcgc tgccaccatc
     1561 tcggcggcgg agagggtccc gcccgggtgg ccggagccgg cctcggccag catctcgatg
     1621 tcggtgatcc ggaggtccct ggcggcctcc tccaggcggg cgatcaggtc ctcacgggtg
     1681 tcggcttcca tcgcggctcc tgcgtcgggg gaggattcat cggtagtcta tcaacgctgc
     1741 atcgagcctc gatgaccgac cgcaccgacg cctcgaccga ccacgggacc gacgagggga
     1801 ccgagcgact cagggtcccc tggtcgcgct cggaccggcg cctcccccgc ctggtggttc
     1861 ggcccctgca gtccttcctc gacacggagg aggccggcgg cgtcctcctc ctcctcgccg
     1921 cgggggccgc cctgctctgg gccaacctct ggcccggctc ctacgagagc gtctggcaca
     1981 cggacctgat ccttcggctc ggctcctggg agctgagtga cgaccttcag cactggatca
     2041 acgacggcct gatggcgctc ttcttcttcg tggtggggct ggagatcaag cgagagctgg
     2101 tggtgggcga gctccgcgac cccaagacgg cggcgttccc cacggtcgcc gcgctcggag
     2161 ggatgatcgt gccggccgcc ttctacctcg cgctgaacgc ggggggaccc ggggtccacg
     2221 gctgggggat cccgatggcc accgacatcg cgttcgccgt cggcgtcctg gccctcctcg
     2281 gcagggggct cccctccagt ctcagggtct tcctgctctc gctggcgatc gtggacgaca
     2341 tcggagcgat cctggtgatc gcgatcttct acagctcggg gatctcctgg gcctggctgg
     2401 ccttagcggc cgggttcctc gtcctgatcg cggcggcaca gcgtgtgtac gtgcgattca
     2461 cggccgtgta cctgatcctc ggcgtgggca cgtggctcgc cgtgtacctc tcgggggtgc
     2521 acgccacgat cgcgggggtc gcgctgggac tcctcacgcc ggccatgccg ttccagatgc
     2581 cgggcccggt gagcgagcgg gcgcgccgcg tcgccgacag gaccgcggac accgtggagc
     2641 acgccgacga ggacgcccac cattggttgg ggctggcgag gctctcccgc gaggcggtct
     2701 ccccactggc ccggatcgag cacgccctcc acccatggac gagctacgtg atcatcccgc
     2761 tgttcgcgct ggccaacgcc ggcgtcgagc tgggcggcgg agcgatcggc gaggcggcga
     2821 cgagccgggt gacgctgggg gtgatcctcg ggctcgtggc cgggaagatc ctggggatca
     2881 cgctgttctc gtggggagcg gtgcgcctcg gcttggcccg gctgccctcg ggctctgggt
     2941 ggacccaggt cacggccgtg tccgccgtgg ctgggatagg cttcaccgtc tcgctcttca
     3001 tcgcctcgct cgcgttcacc gatcccgaga tcctggccgc cgccaaggtg ggtatcctcg
     3061 cggcctcgat cgtggccggc gcgatcgggg cggcgctcct ggcgatgtcc aggcggcggc
     3121 gcgccgccga ccgcgagccc gtcgcggagg gcggggcggc cgtctagcct tcgccgccgt
     3181 cggcggcgcg ccgcctcagc tcgacctcga tcagccgatc gaggtcgccg tccagcacag
     3241 cgtccacgtt ccccacctcc aggttcgtgc ggtggtcctt caccaggcgg tagggatgaa
     3301 ggacgtagga gcggatctgc gacccgaact ggatctcgcg ccgctcgccc cggaggttct
     3361 cgatcctctc ctcgcgctcc cgacgagcga gctcggccag acgagccttg aggatccgca
     3421 tggccaccgc ccggttctgc atctgggagc gctcgttctg gcacgcggcc gtgatccccg
     3481 tggggaggtg ggtgatccgc acggccgagt ccgtgacgtt gacgtgctgg cccccggccc
     3541 cgctcgaccg gtacgtatcg atccgaaggt cgttcgggtc gatctcgatc tcggcgtcct
     3601 cctcgatcac cggcaccacg tcgaggctgg cgaacgaggt gtgtcggcgg ctcgacgcgt
     3661 cgtagggcga gatccgcacc aggcggtgga ccccacgctc cgccgacagg gtcccgtagg
     3721 cgttcctgcc cttcacggtg aacgtcgcgg acttgatccc agcctcctcc cccgggttga
     3781 cctcgtccag ctcgacctcg aagccctgcc gctcggccca ccgcagatac atccggagca
     3841 gcatctcggc ccaatcttgg gactcggtgc cgccggcccc ggcgtggagc gtcgcgatcg
     3901 cgtcggcgtc gtcgaactcg ccggagagga ggacggccag ctcgaggcgg tccacgtcgg
     3961 acgcgagcgc ctcgaggtgc tccttcagct cccgctcggt gtccgggtcc gcctgctcgt
     4021 cgaggagctg gtccagcacc tcggcctcct cgagccgctc ggcgagacgc tcgtagcgtt
     4081 cgacgtcgtc gcggagacga gcgagcctgg tcgtcagctg ctgggcgcgg gcatggtcgt
     4141 cccagaggga ggggtcggag agcagctgct ccagctcggc catctccgcg cgcttggcgt
     4201 gcaggtcaaa ggtactcctt cgcgctcgag aggcgctcgg aaaggccccg gatgcgctcc
     4261 gcgtgctcgc tcatggcgct ccgaatggta gccgatgccg ccgatcggga aatggctctg
     4321 attgactata gggagctggt caggggcgcc cgatactggg tttatgaggc gcaagtcccg
     4381 gtccgcggcc cgcgatcgtc tgcacgacgc attggaggcc ctgcagcgcg acggcgagcc
     4441 gatcatcctc cgcgccggtc gtcgcccggt cggcgtcctc atcactgtgg acgactaccg
     4501 gaagcgcttc ccggggtccg gcgacggaag cgcctcgaac ggcgacggcg gcacctggcc
     4561 ccaggtggag cggcggaagg gcgaccgtcg gcgcggcgag cggaggggcc gccggaggga
     4621 ccccgccccc cgggaggggg accccggcgc caccctcgac gtcctgcgcg agctccgatc
     4681 cctcggcatc acggccgaac gctagctcct cgccgcgtgc tcaggcggcg ccgtggcact
     4741 tcttgtactt cttgccgctg ccgcagggac acggggcgtt ccgggggacc tttccggagg
     4801 tgccggcgcc cggagaggcc gcctcctgcc ggtcgccgtg ctgagccacc atccgctgcg
     4861 ggcgcgggcg cgagggctcg tcctcccgca ccacctcgac gcggtagatg tagcggacga
     4921 actcctcgac catcgaggcc ttcatcgcct cgaacatcgc gtgggcctcg ttctggtact
     4981 cgacgagggg gtcgcgctgg ccgtaggcgc gcagcccgat cccttcctgg aggtagtcca
     5041 tctcctggag gtgctcccgc cagaggttgt cgatgatgtt gaggaacacc atccgctcca
     5101 gctcgcgcat cgtctcctcg cccagctcgg cctcccgctt ctcgtaggcc tcgagggcct
     5161 cctggagcac cttctcggac agctcgagag gatcttcgat cggcttgaga tccgcgatcg
     5221 tcagatcgct cgggtaggtg aggtggatct gggtcagcag cccctcgaga tcccagtcct
     5281 cggcgaagac ctcggggctc acatactcgg cgaccacccc ctggatcacc tcgcccacca
     5341 tatccagcgc gcggtcctgg aggctctcgc cctccaggat gcggcgccgg tctgcgtaga
     5401 cgatcttccg ctgggtgttc atcacctcgt cgtacttcag gacgttcttc cgtcgctcga
     5461 agttgacccc ctcgacctgg cgctgggcgc tctcgatggc gcgcgtcacc atcctggccg
     5521 tgatgggctc gtcctccggc cagttcaggc ggttcatgat cgagccgatg cggtccgagg
     5581 cgaacagccg catcagatcg tccccgagcg agaggtagaa gcgggactcg ccgggatcgc
     5641 cctgacgccc cgagcgtccc cgcagctggt tgtcgatccg gcgtgactcg tggcgctcgg
     5701 tgccgaggac gtagaggccg ccgaggtcca ccaccacctc gtgctcggcg tcggtcgtct
     5761 gcttgtgacg ctcgtacagc ggcccgtact cctcctcgta ggccttccgc tcctgcgggt
     5821 ccatctcgaa gaggaggtac cggtcgttgt cccaaccctt ggccgccatg tcctggcggg
     5881 cgaggtactc ggggtttccc ccgagcatga tgtccacgcc ccggccggcc atgttcgtgg
     5941 cgaccgtgac ggcgcccttc tggcccgcct gggcgatgat catcgcctcc ttctcgtgct
     6001 gcttggcgtt gaggacgttg tgggtcacgc cccgccgctg gagcagcttc gagacgtgct
     6061 ccgacttctc gatcgagacc gtgcccacga ggatcggctg accctccccg ttccgctcga
     6121 cgatgtcctc gatcagcgcg ttccacttcg cgtcctcggt cttgaagatg aggtcgtcct
     6181 cgtcgcgcct gatcatgtcg cggttcgtgg ggatcgtgac gacggggagc ttgtagacct
     6241 gctcgaactc ggcggcctgg gtcttggccg tcccggtcat ccccccgagc ttctcgtaca
     6301 tccggaagta gttctggatc gtgatcgtgg cgagggtctg gttctcctcc ttgatgcgga
     6361 cgccctcctt ggcctcgatc gcctggtgga ggccctcgga gtagcggcgt ccgtggagga
     6421 cccgcccggt gaactcgtcc acgatcagca cctcgccgtc ctggacgatg tagtccacgt
     6481 cccgcttgta gagctccttg gcccggatcg cgttctggag gtggtggacg agcggcgtgt
     6541 tgacgtgctc gtagaggttc tcgatcccga ggtagtcctc gacgcgggcc acgccgtcct
     6601 ccgacaccgc caccgtcctc ttcgtctcgt cgatctcgta gtcctgatcg cgcttgagcc
     6661 tcggggcgat gcgggcgaag gtctggtacc acttcgcgga gtcctggacc gggccgctga
     6721 tgatgagggg ggtcctggcc tcgtcgatca ggatcgagtc cacctcgtcg atgatcgcga
     6781 agtggtggcc gcgctggacc atgtcctcgc gccgcatggc catgttgtcc cggaggtagt
     6841 cgaagccgaa ctcgttgttc gtgccgtagg tgacgtcggc cgcgtagttg ggacgccgct
     6901 cctcgggccg catctgcgac tggatcagcc ccaccgagat ccccagcgcg cggtagatcg
     6961 gccccatcca ctcggagtcg cgcttggcca ggtagtcgtt gacggtgacg acgtgcacgc
     7021 cgtcgcccgc cagcgcgttg aggtagatcg gcatcgtgga cacgagggtc ttcccctcgc
     7081 ctgtcttcat ctcggcgatc ttcccctggt ggaggacggt ggcccccatg acctggacgt
     7141 cgtagtggcg ctggccgatc gtgcggcggg cggcctcccg gacggcggcg aaagcctcgg
     7201 ggaggaggtc gtcgagcgtc tcgccctcct tcaggcgcgc ccggaactcg tcggtcttgc
     7261 cccggagctg gtcgtcggtg aggtcctcca tctcgggctc gtacgaggag acgagctcga
     7321 cccaatgaga gagccgcttg tgctcgcgct tctccccgat ctgctggacc ttgctgaaga
     7381 ccaccctgag gatcctccct gcggactggc ttccgattat acggtcgggc tcgccacgcg
     7441 cggctcccag cgtcccgggc tcggctcgcg gagcggcacg agcaggacga cgacgagccc
     7501 gaggagggcc aggccggcgc ccccgagagc cgggaggccc gcggcccgga gcaggctcgc
     7561 ccccgcgatc gcgcccacgc ccgacgccag cgaggcgacc aggtcggtca ccccgagcgc
     7621 gcttccccgc tcccagggcg tggccatgtc gctcacggcc gccgtcgacc ccagatagga
     7681 ggccgaccac cccatcccga tcaggacgag cccggtcccc gcaccgatgg gccgggtgaa
     7741 caccacgccc agcagcacgc ccgaggcgct gagcgcggcc ccgacgagga ggcccgcgcg
     7801 ccggccccag cggtcgatca gcgccccgaa ggcgggcgag aacgcgaaca tccccgcgag
     7861 atggaggctg acgacgatcg agacggtcac ctcgctcgcg ccgtgacggt ggatcgagga
     7921 cgggagctgg gccatgaacg tcaccatgac ggcgtgggcg atcccgatcg ccacgacggc
     7981 cgtggccgcc gggcggagac ggaggatctc gcgggggctg cgcggagcgg cggtcggcac
     8041 gggccccggg gcggcgagct cccgagggtc cgggcggatc gccgcgacga gccccgcggc
     8101 ccccagcgcc acgacgggga cgagcagcca cgggaaggcg agcggatccg ccaccccggc
     8161 cgaggaggcg agcgtgtgga tcgccccggc cagcaggggg ccgccgatgg cgccgaaggt
     8221 gccggcgacg aggaggagcc ccacggcacg gccgcggcgc tccggcgggt acatgtcggc
     8281 caccgcggcc ctccccagga gcgccgcggc cgtgccggcc ccgagcaggc ccccggccgc
     8341 gtagaggagc ggaggcgagc cggcccggac cgcggcgaag gccagcaggc ccgccaccgc
     8401 gttgagcacg tagcccccga ggaggccggg cttccggccc aggcggtcca tgaccctccc
     8461 cgtcacgagc gcgctcgcag ccgccgcgag agcgtagacg ccgaagagct gggccgcggt
     8521 gccctcgcgg ccggtgagct cctcgatcga cagcggcccg aacgccgcgt acgcgccgtt
     8581 cccggcccac agcgccatct gggcgccggc caggagcagc gtggcgcgcc gaaccgcgcc
     8641 cacgcgatcg tctcgaacga cggccaaccc ggatcctccc cctcgagcgc ggccgagcct
     8701 acttgatctc gagcagcttc tcgcgcaccg cgtacaccac ggcctccatc ctggagtgca
     8761 gctggagctt ctccaggatg ttgcgaacgt ggttcttcac ggtgttctcg gagatgaaca
     8821 gctccttggc gatgtcgcgg ttgttcatcc ccttggccac gagacggagg acctccatct
     8881 cgcgatcggt gagccgaggc gtgtggacgc tctcccgctc ctcggcccgc ttcgacatcg
     8941 cctggaactc gttgaggagc ttcgacgcca tggccggaga gatgcgcgac tgcccggtcc
     9001 acaccgagcg gatggcgtcg gccacgtcct cggtcgagat ctccttcagg aggtagccgc
     9061 tggcgccggc cttgatggcc tcgtagaggt cctcctcctc gtcgctcacg gtgagcatca
     9121 ggatcttggc gtgcgggatc gcctccttga tcgcggaggt ggcctcgatc ccggtgcgct
     9181 tgggcatgcg gacgtccatc aggacgacgt ccggcatgag ctcttcggcc ttctcgacgg
     9241 cctcctggcc gtcggtggcc tcgccaacga cctcgatccc ctcctcctgc tcgaggacca
     9301 tctggatgcc gcggcggaac agcgcgtggt cgtcgacgat gaggaccttc agcgcatcgc
     9361 cgtccgcctt ctcagcggcc ttcttcgaca tccatcacct cggggggctc cgggacctgg
     9421 cgggatttta cccgattcgc tccttcgatc agtttcctgc gccgcttccc ggcgtgtccc
     9481 gcgagctggc gatccaggcg cgagacgacc tgctccaccg cgacctcgag ggtctccccg
     9541 gcgccgctgg cgtggaagga gcgccggtcg gtccaggccg acccctccac ccggtgcccg
     9601 gcgctcaccc tcgggttcgc ctcctggatc acgttcacct ccacgcgccg aaccctccag
     9661 tcgtggcgcg cgagcctcgc cgccttccgc tccacgacct gacgggcgcg ggcggggacg
     9721 cgccccttcg tcgccttcac cacgacgtcc atggcatccc tcctcagcgg gcccccgtct
     9781 caggggagac ggacgagatg gaacccgcgc ccggctgacc tggtctttgc ccccacactc
     9841 gcctgctgag gggttcgccg ccctgaggac ggcttagcac cttctcccgg cgtaccgggg
     9901 caggacttac atctaagccg caccatgctc agcaaccaag ggttgcctta cgtgggtcgt
     9961 ttcgcctacg gctggcatcg actaccgggg aacatccccg ggcaaccaca gacccggaca
    10021 cgggttccat ctcgcgccat catagccgag gagcggcccc ccgagaaccg ccgaaggtcc
    10081 cggcggggaa ggggccccgg accgacctcg ccgcggtggc caccaccacc cgctcggtcc
    10141 ctccctcccg cagggccgcg gcgcaggcgg aggcggtggc tccggtggtg agcacgtcgt
    10201 ccaccaggag gaccgagcgg ggaacccggc gtcgggcccg gaacgcgccc acgagggcgg
    10261 cccgccgctc ggcgccgccg agccgggcct gggccgccgt gtcgcgccgc ctccggaggg
    10321 ccgcgatcgc ggggaggccg aggaggcgcg cgacacggcg gcccagcacc tcggcctggt
    10381 cgaacccccg gcggcgccgg cggcgggccg acagcgggac ccagcagacg gcgtcggtct
    10441 ccgtctcggg cagcacagcg ccgatcgcgg cggcgagccg cggcgccagg gctcgccacc
    10501 ccccgaactt catgccgcgg atcgccgccg cgatcggccc ctcgtacagg aacgggctgc
    10561 gggccaggtc gatccccccg ggagggcagt cggcgcacac ctccaccggc gccgcccagg
    10621 gccgcccgca ccgctcgcac agcgggggca cgatcacccc cacatcctcc acgcaccgct
    10681 cgcagagcag gacccttcgc cgcgagcagc ccaggcacct cggagggaag atcgcgtcga
    10741 gcaccggccc agcctagcga gggggtccga cgcgaaagct caggggagcg agagggtggc
    10801 ggaccagggg cctcgctgga tcggtttccc ctggcggtga cgccgatcca gctcgagggt
    10861 gagctcctcg tctccccgca tcactgggta gatcgtcgtg gtccgcgacc ccgtgctcca
    10921 ctcgtactcg tcgtccagga cgtggagccg gcgacgcccc gccgtcacct tcaggtcgcc
    10981 gaactcccgg aatccgggct tctcggccgc cgcgtgccgc cagtggacgg ccgccgagtc
    11041 cgcccggaag acgagccggt cgatcgcggc tccacggctc cccggcacct tgatgcggat
    11101 cccggcctcg acgctccccc gccgcacggc ctcgctgggc caggccacgc agaaccggcg
    11161 ctcggcctct cgcgggcccc gctcgctctc ctgcccgtcc acgtccacct cggcctggac
    11221 cgtgtaccag ccgggctcca ggtcggagaa cggaacctcg aacgggatca tcacgcggtc
    11281 gcgcggggcc acgtcgacgg tgacctggcc cagctcgagg ttccggacgg cgcgagaggg
    11341 gccgacctcc accaccaccg cctcggtcag gcggacctga tgcgggtcgg ggtcgacccc
    11401 gcgcacgacc acggcgcccc gcaccgaggc ggggaaccgc tcgaacaccg cgtcgacgct
    11461 gaccccgctc aggtccccat ctcccacgaa ccgaggtagc gctcctgctc gggggtgagc
    11521 tcgtcgatct cgatccccat tgcccggagc ttcagggcgg cgacctcggt gtcgatctcg
    11581 tccggcaccg tcaggacctc gggctcgagg ccctcgcgcc ccgccagcca ctccacgacc
    11641 agggcctggt tggcgaacga catgtccatc acggccgccg gatgaccctc cgcggccccc
    11701 aggttcacca gtcgcccctc cgccaggagg gcgatgcgcc gcccgtcggg catcgtgtac
    11761 tcgtccaccg accctcgcag gcgccgcggc tctccaccga gcctcgagag cgcctccttg
    11821 tcgatctcga cgtcgaagtg cccggcgttg gccaggaccg cgccgtcctt catcgcctgg
    11881 aggtgctcct cgcggatcac gctcgtgtca cccgtgaccg tgacgaagac gtcccccacc
    11941 cgagcggcct cgcgcatcgg catcacgcga tagccctcca tcgcggcctc cagcgcggcg
    12001 aggggggaga cctcggtcac cacgatctgc gcgcccatgc cccgggcccg ggaggcgacc
    12061 cctcggccgc acatcccgta gccgcagacg accaccgtgc ggcccgccaa caggaagttc
    12121 gtggcccgca tgatcgcgtc gatcgtcgac tgccccgtcc cgaaccggtt gtcgaagagg
    12181 tgcttcgtca tcgcgtcgtt cacggcgacg atcgggtagc ggagggctcc cgccttggcc
    12241 atggcccgga ggcggatcac gccggtggtg gtctcctcgg tccccgcccg cacctcacct
    12301 gcctgccccg cccgctcctc gtggaggacg ttcacgaggt cggcgccgtc gtccatcgtg
    12361 acgtggggcc tcgtgtcgag gaccgccctg atgtgcgcgt agtaggcgtc gttgtcctcg
    12421 ccccggatgg cgaacgtggc gatgccctcg tcgcagaggg ccgcggccac gtcgtcctgc
    12481 gtcgagaggg ggttcgaggc gcaggccgcc acctcggccc cgccccctcg cagcgtccgc
    12541 aggaggttcg ccgtctccgt cgtgatgtgg agacaggcgg cgacgcggag tccgtccagg
    12601 ggacgctcct tctcgaagcg ctccccgatg aggccgagga cgcgcatctc gcgtccggcc
    12661 cactcgatgc gctcccggcc catctcggcg agaccgggat ccttgacgtc acacacggct
    12721 cactcctctc ccagggcggg caggacccgc cgcagcagct cgcgcaggcc gtcggcgaac
    12781 ctcgccgcca cccgcgtcac ctcctcgtgg ctcacgtgga cccccacgcg gttcgtgacg
    12841 cagaagaggc ccaggcaccg catccccagc gccgccgcgg cgcacgcctc gggaaccacc
    12901 gacatcccga cgacgtcgcc gcccagccgc cgcagcatct cggcctccga cggcgtctcg
    12961 taggtggggc ccggcatcgc agcgtacaca cccctggcga acgggagccc cacctcgcgg
    13021 gcggcccgct cggcggcctc cgcaagcccc ggagggtaca cccgcgtcag gtccacgaac
    13081 accggccgac cctcctcgtc gcgccagccc cggagggggt tctcgcccag gaggttcagg
    13141 tggtccgagg cgatgacgag cgagccaggc tccaggtcgg gatcgagccc gccggtggag
    13201 gcggtcacga cgagcgtgcc cgcaccgagc gcggccgcga gccgagcggg cagggtgacg
    13261 agcggcatcg ggtgcccctc gtagaagtgc accctcccca ggaacccggc gacggggacc
    13321 tccgccagcg tgccgacgac caggcggccg gcgtgtccgg gcacagaagg cagggggaag
    13381 cccggcaggt ccgagaaggc gagctcggca tcgggctcga tcgcgtccag cgcgtcgccc
    13441 aggcccgatc ccgcgatcac cgccacctgc gggcggaggt cggtcacggc ccggacggcc
    13501 tccgctcccc gctcggccag atcgtcgccg gggccgaccg tcatcctcgc cccccggcca
    13561 ggcgccgctt cagccgatcg atcgcctcga tcggcccggg atccacgccc cgggccaggg
    13621 cgtggtagac gctcgccacg tcgcccagga ggatcagcga gagcgaatgc gcgagcgggc
    13681 cgcgtccccg ggcgtgggcc tcgcgccagc cgatccccga ctcctcgatc gcctcgaagg
    13741 tcgcctcgag cctggggccc acggtccggt gctccccggg atggcgcaga gcgatcacca
    13801 ggaagccgtg gccccgcccc ttcgaccacc ccgcgacctc gtggtggtcg agctcgggga
    13861 gcgccgagga caaggccggg atcttcgcgt tctcgtggaa ggccgccttc caccggaagg
    13921 ccgccggctc cgccagcccg tccgatcccc agatgaccgg cacgtgctgt gacccgaccg
    13981 cgcgggccac gtccatcgcc tcgttctcgt cgcggcccgg ggccagctcc ccggccagct
    14041 cggccagggt ctcggcggtc tccttcacct ccgcgtccgc ctgggggagg aggcccatcg
    14101 cgacgagggc gcccagcgcc ccgcccgcca gcaggccgag tcccgcccga ggcatgggcg
    14161 cggcggcggg gatcgtgacg gcgccctccc cggccagctc gatcaggcgc cccccggagg
    14221 cgaccgccgt caccgtggat ccccgccgcc gcgcctcctc gaggcaggcc accgtctcct
    14281 ccgtctcccc ggagaacgag aggcacatca cgaggtcgcc ggggccgcac caagcaggca
    14341 ccgaatagcc gcgcaccgag gccaccggga gccggagccg gtcggcgaag gcagacgcca
    14401 cgacgtcgcc ccccgccgcg gagcccccca tgccgcagat gatcagcgag cgaggctcga
    14461 ccgaggagag cccggccgcc cggccggcct cgtacccctc gacgagctgc tcggggagcc
    14521 gcaggaacgc ctcgagcatg ccgctcgggt cgccggcggc gatggcggaa gcgtcgatcg
    14581 gggatcgcat ccggctagcc gccccccgag ggcttcaccg cctcctcgat cagcatcacc
    14641 gggatgtcgt cgcgcacggg gtacttgtag ccgcacttcc ccaggcagac gatgaacccc
    14701 tcctcctccc ggtactcgac ctggccccgg cagttgggac acgccaggac gtccagcagc
    14761 tcctgatcca gcgccatctc actcccctct gatgttcgcg aggagctccg cggtcctctc
    14821 cgcgaggagc ccctcgtcac gagcttccac gttcaggcga agcagcggct cggtgttgga
    14881 cggccgcgcg ttgaaccacc agtcctcgta ctcgaccgtg aggccgtcga gccggtcctg
    14941 ctcgccgtcc gtgtagcgct gagcgagacg gtcgatcgcg gcgtcctggt cctcgacctc
    15001 ggagttgatc tcgccgctgg cgtggtagcg gcggaacggc cgcagcagct ccgagagcgg
    15061 gaggcccgcc tcggacatct gctccagcac caggagcgcg gccacgagcc ccgagtcggc
    15121 ccggtagtgg tcccggaagt agtagtggcc cgagtgctcc ccgccgaaga tggccccggt
    15181 ctcggccatg acctgcttga tgaacgagtg gccgacccgc gtgcggaccg gggtgccgcc
    15241 gctcgcccgg atcacctcgg gcacgaccca cgagcagatc acgttgtaga ggatcgtcgc
    15301 gccgggctct cgctccagca tcttgtcggc gatcagggcc acgaggagcg acccggagac
    15361 cccgttcgcg ccctcgtcca cgacgaagac ccggtcggca tccccgtcga aggcgagccc
    15421 cacgtcggcg ccgtgctcga cgacggcctt ccgtaggtcc tcctggttct cgggctggat
    15481 gggatcggca gggtggttgg ggaacgtgcc gtcgaggtcg aggtagaggc cgatcagctt
    15541 cgccggcagg cgctcgaaga ttggctcgag gacgagtccg gccatcccgt tggccgcgtc
    15601 ggccaccacg gtgagcggag tgagcctcga cacgtccacg aagcccacga cgtggtcgac
    15661 gtacgcgtcg agcatcgacc gctccgcgac cgagcccggg gcacccgagg gagccaagtc
    15721 gcggtcggcg agatcgcgga tctcgcggag cccggtctcc tccccgatcg gcgccgcccc
    15781 ctcccgacac agcttcagcc cgttgtactc gggagggttg tggctcgcgg tgaacatcgc
    15841 tccgggaaga tcgagggagc ccgacgcgaa gtagagcatg tccgtggtgg caagaccgat
    15901 gtcgacgacg tcggccccgc gcccgacgac cccctcgatg aaggcccctg agagatcggg
    15961 ggaggagatc ctgcagtccc tgcccaccac cacccgctcc gcggcggtga actcggtgaa
    16021 cgccgcaccg atgcgccggg acgtgtcctc gtccagctgg tccgggtaga tgccgcggat
    16081 gtcgtacgcc ttgaagatct ccggcggcgc catgccgcgg attctatccc aggcgccggt
    16141 gagggccctt ctcaggcggc ggtgacgagc gggatggcgg tctcgcggtc gatcaggtcc
    16201 ccgtcctgct cccaccactt cgcctcacat ggagggcagg tccggaagac gatcggcgag
    16261 tcaccgaccc cgaacgtcac gacgacgacc tcactcgatc cgcagccggg gcacgtggtc
    16321 aggggagccg cggcgtgggc cttgctcttg atcgcgctca tacccaggcc atcggccccc
    16381 gggcgccttc ccctgcatag cacgaagggg tcatttcacc gcgcaccggg cgtgggcgga
    16441 gagggcctcg atggatcagc ctcgtgtccc ccgcccgacc cacagcgcgg acgcgctccg
    16501 cgtcagcggg gccggtcctc gggaagctcc ttcccgatgt agcgagaccg cagacgaccg
    16561 tcctcccgcc agtaggcgta ccagtagggc ccgtggggac accggccgca gctctctcgg
    16621 ccgcatcgaa cgagctcctg tcggtaggtc accttgccgg cccgggcggg ctcctccatc
    16681 tcgaagtcgt cgttccggcg ggccacgagc agcccgcgaa cgaagatcat caggcgccgg
    16741 aggtcgtatt cgtccagcgc gcggatccgc cggatcaggt cgtggtcgag catcttgggt
    16801 acggaagtgt acccaagacc cgctgatcag gccgaagcgt ggaccgggct cgggagctcc
    16861 ggggcgccgg ggcggtgatc caccaggcgc cagcccctcg gggccgtgag gttcgaggcg
    16921 tgcggctcgc agagatccag caggttgggg tcacgctccg ccgagagagg ctcgacgatc
    16981 gcggtgcggt cactgtagcg gacggcgacc gacgcggccg cgacctcctc acagcgcatc
    17041 ttggcgcagc gtcgcatggg cggagggtaa accacatcga tgtcgttcac aaggatccgc
    17101 gattaggtaa cagtttcgtt acctaagaac gggaacagga tgcgccggcc ccacgggatc
    17161 gtggggtcgg gaaaaagcga ggcaggggcc ccaagggctc cctgccgcgg aaaagcgtac
    17221 tccgaagcga ctgtccgggt tgccccgtcc cgtctctccg gagtactcca ccacactaca
    17281 ccggggggcg gggatgatgc aacccctgga tcgggtcctg tcacacttcc tacgggagga
    17341 tgtgcacctt ggtcccgatc gggatcatgc cgaagagctc cttcgactcc tcgatccgca
    17401 tgcggatgca gccgtgcgag gcgaaggtcc cgatcgtgtc ctcggcgtag gtgccgtgaa
    17461 tcctgatgcc cggcgcgtcc aggtagagcg cgtgcgtgcc gagcgggttc ccggggcccg
    17521 ggccgatgac ggcggggaga tcggcgcccc agccgtcggg agcggggttc acccacacgg
    17581 ggttctctcg cttgtcccag atgttccagg tgccctgtgg cgtggggtac cccccggtgc
    17641 cggtggcgac gtcgtaccgc ttgatcacct tgaacccgtc gtagaggtac aggcgattct
    17701 cggagatccg caccacgatc gagagcccga ggccgtcggc ggtgacatcc ggctcgacct
    17761 tggagaccgg gagcgtcacc agaggcttcc cctgtcgcag cgcggatcgc accctctcac
    17821 gggagcgctt gacgtcgagc tcccgcccca tcttcggtcg ggtgaggacc agctccccct
    17881 cctcgatgcc gaacgacgcg tcgttcggcg acacgtacgt ggcctcgctc acgatcccga
    17941 ggaagcgctt gatcatctcg ccgtccctcc ggagccggac ccggaggtcc tcctgtaccg
    18001 gacggtcgaa gatcctggcg aaggcccgtt cgtgccaggc catcccctcc cccacggcga
    18061 gcgctcgatc caccttctcc tcgatgtcgc ccctggttcc gagctcggcc ggcgtcacgt
    18121 gccaggagcg gccccgggcc cgcacctcga tctcctggga gagatcggac tcgatcgcct
    18181 ctccaaccgc cgcgatcgcc tcggcgcgcg tcatctcccc cacgtcgacg ccggcgatcg
    18241 tcacgcccgg gaggatcctc ccggtctggg cacgatcgaa gcgatacgcg ccgtatgcga
    18301 cacccccgcc gagcaccatg agaccgacca gcgtgaacac gacgacgcgg agcaggcgtc
    18361 gtcccgcagc ggcgtgtcgt cggcctcgca tggaggcgat gataccctgc ggcctccctt
    18421 ccgaacaggg cgttcagggc agatcaagtg gggcgccgga tgcgacggcg tacccatcgg
    18481 ggtcccgaga ggcctggccc gcgatcacgg tccccgactc ggcccgcacc accaccgaca
    18541 cgggctcgtc cccgacgaac tgcgagaggt cgaccaggcg gtagcccccc ggtcggaggg
    18601 cgatccgccc gatctccgga gcttccgccg gacccgtcgc ggtcaccagg acgatccgga
    18661 cgtccgcgac ctcctccccg gtgttctgga ggaagagggt cgacgggccc cctgcgggag
    18721 cggtcgtggg gagcgcgacc cacgacgccg ccccctccac gatagcgggg acaccggcga
    18781 ggtcgccggg ggtgttcagc ctccgcccgg ctacgaagag ctcggacccg tccgcgaccg
    18841 cgacgagccc cgccccctgg gcgggcacaa cgtgagtctc ggccttcccc ggagggacca
    18901 cggcctcgga gatcacctcg agctgtcctt ccgtcccctg gacacgggcc cggaaggggg
    18961 cctccgcgcg gctcgtccct aggacggtca gggcgctcgg ctcgtcgtcg agcgcgcccg
    19021 gcatcaccca catcccggcc gacgccgtga cccccgtcga agcccggatc cccgtctcgc
    19081 tcaagcccac tccccccacc gcgaccttcc ccaggccgac gtccacggtc acggccagcg
    19141 tttccttccc cagcgcgaag gccccgaggt cgaacgaggc ggaccggcgg cctggcagca
    19201 cgatcccttg gaggtccccc ggcttgatcg tctcccgctc gtcggtaagc cacaggctga
    19261 ccaccgcctc ctgcgggaag gggttcatca cgaccacgcg ctggtcgaag ccccggaggg
    19321 tggtcccctc cgggaggtac cagcgggtcg ccgactcggc caggcagggc tcggcggcgc
    19381 ggcccagcgg ctcccctctg ccggtgctcc gcatgatcac catccccgcg gcgacggggc
    19441 ctccgaagaa ttcgacggcc gacgcggccc gcgggtcgga cgccgggacc tcgacgtacc
    19501 gctgggtccc ggggccgacg gtggctacct gagactcggg acgctctccc ccgtacgtgg
    19561 tcgcccggat ctgggccggc tcctgggtcg ggttggccac gaccagccac acgcgccagc
    19621 gaccactccc accgtgcgga cagaaccagg ctcccgagac cgcggacggc acctccccgg
    19681 gcgtcagggt gcgagccccc agccctcgct ccagggccaa gcccccggcc acgacgagga
    19741 gcgatacgag gagcgccccc ggaaggcgca gccgctccac ccaggggcgg ctcatcctcg
    19801 cctccggagg atccagagcg ctccgaccca gaccaccagg agcccggcca cctcggccgt
    19861 tcggacccac ccctctctac gatccagcgt caaccgaccc gatcggggcg gcgcctccac
    19921 gccgatcgcc catccgaagg cgcggaacgg ctcgtactgc cgcccgtcct cggcacggag
    19981 gacccacgcc ggctcgtact ccttggaaac gaaggccgtg ccggtctcct ccaggtccgg
    20041 ggcgccggtc cacgtgttcg gctccacgcg ccggaacggc gccatcccca gccgcgggac
    20101 ccgcgcaggc gcgagggcgt ccaccgcccg cacgtcgccg tcgggcgggg cggcggggag
    20161 gatccccgcc aggggcagcg ccctcgtgtt ccggtacagg acgagtccgc ccgcacgagc
    20221 ccccggcagc aggtcgagct gcgcgtccag cctgagccgg gcctcgggcg ggaggtcgcc
    20281 ctcgcccgcc accacgtagg ccacggcgaa aggcgcgagc agcgagccgc cgtggcggat
    20341 gcggccggcg agcatcgtg
//
LOCUS       DASUTZ010000017         4642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_263431, whole genome shotgun sequence.
ACCESSION   DASUTZ010000017 DASUTZ010000000
VERSION     DASUTZ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263431"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VF097_00760"
     CDS             <1..209
                     /locus_tag="VF097_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HEX6261355.1"
                     /translation="GPYASIYTGCEITGSEVEDSIVMERCRIHGVPGIAESLLGKDVE
                     VSRGKSGRAAHRLMLGDQSQVEVL"
     gene            234..698
                     /locus_tag="VF097_00765"
     CDS             234..698
                     /locus_tag="VF097_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012901462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEX6261356.1"
                     /translation="MADRIDGVWVKDLRFIPDERGRLIEILRSDEEGFRKFGQVYMTT
                     TYPGVVKAWHLHKVQDDNFCCLKGMVKLVMYDARDDSPTKGTIDELFIGDHDPKLVRV
                     PPGVYHGWKCVSEEEAIVLNVPTEPYNHEAPDEYRAAWNTPDIPYSWDIVFK"
     gene            695..1642
                     /gene="rfbB"
                     /locus_tag="VF097_00770"
     CDS             695..1642
                     /gene="rfbB"
                     /locus_tag="VF097_00770"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014179435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEX6261357.1"
                     /translation="MNILVTGGAGFIGSNFVDLAMADPSRRVTVLDALTYAGTKENLE
                     GHRKNERLRLIVGDVADPEAVRESLDGADLAVHFAAESFVDRSIEAAEPFVRTNVQGT
                     LTLLTACRDAGVPMIQVSTDEVYGSIEDGAFTEESPLAPNNPYAATKAAADLLCRSFR
                     ATFGVDVRILRGTNAYGPRQHREKAIPVFIAAALATRPLPVYGDGANRREWLHVTDFA
                     RAVWTVVEQGEPGGVYNVGGGHETSNVDLAREICRLAGASEDLVSFVEDRPGHDLRYS
                     MLWERIAALGWTPAVPFDEGLAGTVEWFRAHPERLEGSS"
     gene            1639..2478
                     /gene="rfbD"
                     /locus_tag="VF097_00775"
     CDS             1639..2478
                     /gene="rfbD"
                     /locus_tag="VF097_00775"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEX6261358.1"
                     /translation="MKIVVTGAGGGVGRALREAAPGHHEIEWLTHADLPVEDRHAVMQ
                     RVAGADPDAIFHLAAKTKVDDCERDHDAAYRMNALGTANVALAARETGALLVALSTDY
                     VFDGTKSEPYHEFDRPNPISVYGASKLAGEEEARRLAPDHLIVRTAWVFGGGEDFVTG
                     AVRRLASGEEVEAIVDLHGTPTYAPHLAERLFPLAVSGLRGVVHLGGPERTTFFDLVA
                     RARELGGLPGTPVEQKDADLGRPAARPRDSSLRSLVLGGAAVAPMPPLDDALRDLLGR
                     IES"
     gene            2475..3416
                     /locus_tag="VF097_00780"
     CDS             2475..3416
                     /locus_tag="VF097_00780"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6261359.1"
                     /translation="MSATGMQTRTSESPRFKFLERIRAVLGRREVIGNLTGKEMKVKY
                     KSTILGVLWSMLNPLLYLVVFWVVFTVFLPSGIPDFAVFLLSGLLVYSLYSNALQTGT
                     ASVVDNAPLVTKVAFPREVLPLSAVGASLVNFAYQFIVLLLFMIVIGYDFWGWNLLLI
                     PVALAVLLLFTTAVVMGTAALNVRYRDTRHLVELSLLAWFWVTPIVYSASVVMGRLDV
                     WAQRIFLANPLAPIVLAFQRGLYGYRLAGSCGDSGVEDALPARLAEIPGCQAVLPDAG
                     LTWYYWRLGLVAAASLLLIAITWRIFFRMSGDFAEEL"
     gene            3416..4588
                     /locus_tag="VF097_00785"
     CDS             3416..4588
                     /locus_tag="VF097_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6261360.1"
                     /translation="MEPAVSVRGVSKRFKLYRERPTSLKERVVSFRRKERAEDFWALR
                     DVSAEIPTGSTVGLIGANGSGKTTLLKIIGGIIRPTAGEVRTRGRIAALLELGAGFHP
                     ELTGRENVYLNASILGLTRRETDSYFDRIVEFAELERFIDNQVKFYSSGMFVRLGFAV
                     AIHVNPDILLVDEVLAVGDEAFQRKCLDRVALFQEEGRTMIIVSHDADLVARVCDQAI
                     MLDDGRIRAAGDVDEVVKDFHTLMLTIHAPFVREDVTKEVEIRDVRLVGPDRDGIRSG
                     DPLTVQVDIEAKHPVQDPVVSMAIYDGSDRLMFKTGTDWTGLPLGTVEGKRRVSFRIS
                     TVPFTSGRYRLAVGVHSPRLESVYDWHDRQGAFEIRRDPPREGSVWVPHETEVEDL"
BASE COUNT          701 a         1565 c         1635 g          741 t
ORIGIN      
        1 tcggccccta cgcctcgatc tacacgggct gcgagatcac cgggagcgag gtcgaggact
       61 cgatcgtgat ggaacggtgc cggatccacg gcgtccccgg gatcgcggag tcgctgctgg
      121 gcaaggatgt cgaggtctcg agggggaaga gcggccgggc cgcccaccgc ctcatgctcg
      181 gcgaccagtc ccaggtcgag gtcctgtagc gaccctcctg tagactctgg cccgtggcgg
      241 accggatcga cggcgtctgg gtcaaggacc tgcgcttcat ccccgatgag cgcggacggc
      301 tgatcgagat cctgcgctcc gacgaggagg ggttcaggaa gttcgggcag gtctacatga
      361 ccacgaccta cccgggcgtg gtgaaggcct ggcatctcca caaagtccag gacgacaact
      421 tctgctgcct caaggggatg gtgaagctcg tgatgtacga cgcccgcgac gactccccga
      481 cgaagggcac gatcgacgag ctgttcatcg gcgatcacga tcccaagctg gtgagggtcc
      541 cgcccggcgt gtaccacggc tggaagtgcg tgtcggagga ggaggcgatc gtcctcaacg
      601 tcccgacgga gccgtacaat cacgaggcgc ccgacgagta ccgggcggcg tggaacacgc
      661 cggatatccc ctacagctgg gacatcgtct tcaagtgaac atcctggtca cgggaggagc
      721 cgggttcatc ggctccaact tcgtggatct cgccatggcc gacccgagcc gtcgggtgac
      781 ggtcctcgac gcgctgacgt acgcgggtac gaaggagaac ctcgaaggtc atcggaagaa
      841 cgagcgcctg cggctgatcg tgggcgacgt ggccgatccc gaagccgtcc gggagtcgct
      901 cgacggcgcc gacctcgccg tccacttcgc ggccgaatcg ttcgtggatc gaagcatcga
      961 ggccgcggag ccgttcgtgc ggacgaacgt ccaggggacg ctcaccttgc tcacggcgtg
     1021 cagggacgcc ggcgtcccca tgatccaggt ttccaccgac gaggtctacg gctcgatcga
     1081 ggacggcgcc ttcaccgagg agtccccgct cgcccccaac aacccgtacg ccgcgacgaa
     1141 ggccgcagcc gacctcctgt gccgttcgtt ccgagcgacg ttcggcgtgg acgtgcggat
     1201 cctccggggg acgaacgcct acgggcccag acagcaccgg gagaaggcga tcccggtgtt
     1261 catcgcagcg gccctggcca cccggccgct gcccgtgtac ggggacgggg cgaaccgtcg
     1321 ggagtggctg cacgtgaccg acttcgcccg agccgtctgg accgtggtgg agcagggcga
     1381 gcccggtggc gtctacaacg tcggcggggg acatgagacc tccaacgtcg acctggctcg
     1441 ggagatctgc cggctcgccg gcgcgtctga ggatctcgtc tccttcgtgg aggaccgtcc
     1501 ggggcacgac ctccgctact cgatgctgtg ggagcgcatc gcggcgctgg gctggacgcc
     1561 cgcggtgccg ttcgacgagg gcctcgcggg aaccgtcgag tggttccgcg cgcatcccga
     1621 acggctggag gggtcgtcgt gaagatcgtc gtcaccggcg cgggcggggg cgtgggcagg
     1681 gccctgcgcg aggcggcccc gggccaccac gagatcgagt ggctgaccca cgccgacctg
     1741 ccggtcgagg atcgccacgc ggtcatgcag cgggtggcag gcgccgaccc cgacgcgatc
     1801 ttccacctcg cggccaagac gaaggtggac gactgcgaac gcgatcacga cgccgcgtac
     1861 cgcatgaacg cgctggggac ggcgaacgtc gcgctggccg cgagggagac gggggcgctg
     1921 ctcgtggcgc tctccaccga ctatgtgttc gacgggacga agagcgagcc gtaccacgag
     1981 ttcgaccggc cgaacccgat ctcggtctac ggggcctcca agctggctgg ggaggaggag
     2041 gcgaggcgcc tcgcccccga ccacctgatc gtgcggacgg cctgggtctt tgggggcggc
     2101 gaagacttcg tcacgggggc ggtccggcgg ctggcgagcg gcgaggaggt ggaggcgatc
     2161 gtggacctcc acgggacgcc gacctacgct ccccacctgg ccgaacggct cttcccgctg
     2221 gccgtctccg ggctgcgcgg ggtcgtccac ctcggagggc ccgagcggac gacgttcttc
     2281 gacctggtcg cgcgggcgcg cgagctgggc ggcctgccgg ggaccccggt ggagcagaag
     2341 gacgcggacc tcggccgccc ggccgcgcgc ccgcgcgact cttccctccg cagcctcgtg
     2401 ctcggcggcg ccgccgtggc tccgatgccg ccgctcgacg acgccctccg cgacctgctg
     2461 ggaaggatcg agtcgtgagc gcgaccggca tgcagacgcg aaccagcgag agcccccgct
     2521 tcaagttcct cgagcggatc cgcgcggtgc tcggccgccg cgaggtgatc ggcaacctca
     2581 ccggcaagga gatgaaggtg aagtacaaga gcacgatcct cggcgtgctc tggtcgatgc
     2641 tgaatccgct cctctacctc gtcgtcttct gggtcgtctt caccgtgttc ctgccctcgg
     2701 ggatcccgga cttcgccgtg ttcctgctgt ccggcctcct cgtctactcg ctgtactcga
     2761 acgccctgca gaccgggacg gcatccgtcg tggacaacgc cccgctcgtg acaaaggtcg
     2821 ccttccctcg cgaggtcctc ccgctgtcgg cggtgggggc ctcgctcgtg aacttcgcct
     2881 accagttcat cgtgctgctg ctcttcatga tcgtgatcgg gtacgacttc tggggatgga
     2941 acctcctcct gatcccggtc gccctcgcgg tgctgctgct gttcacgacg gccgtggtga
     3001 tgggcaccgc cgcgctgaac gtccgctacc gcgacactcg gcacctcgtc gagctctcgc
     3061 tgctcgcctg gttctgggtg acccccatcg tgtactcggc ctcggtggtg atggggcggc
     3121 tcgacgtgtg ggcccagcgc atcttcctgg ccaacccgct ggccccgatc gtccttgcct
     3181 tccagcgggg tctgtacgga taccggctgg ccgggagctg cggcgattcg ggggtcgagg
     3241 acgcgctgcc ggcgcggctc gccgagatcc ccggctgcca ggccgttctc cccgacgccg
     3301 gcctgacctg gtactactgg cgcctgggcc tcgtggccgc cgcgtcgctc ctgctgatcg
     3361 cgatcacgtg gcggatcttc ttccggatgt cgggcgactt cgcggaggag ctctgatgga
     3421 gccggcggtc tccgtccggg gcgtctccaa gcggttcaag ctgtaccggg agcggcccac
     3481 gagcctgaag gagcgcgtgg tctcgttccg tcggaaggag cgagccgagg acttctgggc
     3541 gctccgcgac gtctcggccg agatcccgac cggatctacg gtcggcctga tcggcgccaa
     3601 cggctcgggg aagacgactc tcctcaagat catcgggggg atcatccgcc cgacggcggg
     3661 ggaggtccgc acccgtgggc gcatcgccgc cctgctcgag ctcggcgcgg gcttccaccc
     3721 cgagctcaca gggcgcgaga acgtctacct caacgcgtcg atcctggggc tcacgcggcg
     3781 cgagaccgac tcgtacttcg atcgcatcgt ggagttcgcg gagctggagc gtttcatcga
     3841 caaccaggtg aagttctact cgtccgggat gttcgtccgg ctgggcttcg ccgtggccat
     3901 ccacgtgaac cccgacatcc tcctcgtgga cgaggtcctg gcggtcggcg acgaggcctt
     3961 ccagaggaag tgcctggacc gcgtggcgct gttccaggaa gagggccgga cgatgatcat
     4021 cgtcagccac gacgcggacc tcgtggcccg ggtgtgcgat caggcgatca tgctcgacga
     4081 cggccgcatc cgggcggccg gcgacgtgga cgaggtcgtg aaggacttcc acaccctgat
     4141 gctcacgatt cacgcgccgt tcgtgcgtga ggacgtcacc aaggaggtcg agatccggga
     4201 cgtgcggctc gtggggccgg accgggacgg gatccgctcg ggggaccctc tcaccgtcca
     4261 ggtagacatc gaggcgaagc accccgtgca ggaccccgtg gtctcgatgg cgatctacga
     4321 cgggtccgac aggctgatgt tcaagacggg gaccgactgg acgggcctcc cactgggtac
     4381 cgtggagggc aaacgacggg tctcgttccg catcagcacg gtcccgttca cgtcggggcg
     4441 gtaccggctc gcggtaggcg tgcacagccc ccggttggag tcggtctacg actggcacga
     4501 tcggcagggc gcgttcgaga tccggcgcga tcccccgcgc gaggggagcg tctgggttcc
     4561 ccacgagacc gaggtcgagg acctgtgacg gagcaccggc acaccgatcc gctggacatg
     4621 tactacgagc ctcccccgcc cc
//
LOCUS       DASUTZ010000018        23721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1688923, whole genome shotgun sequence.
ACCESSION   DASUTZ010000018 DASUTZ010000000
VERSION     DASUTZ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 23721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23721
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1688923"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1671)
                     /locus_tag="VF097_00790"
     CDS             complement(61..1671)
                     /locus_tag="VF097_00790"
                     /inference="COORDINATES: protein motif:HMM:NF005907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HEX6261361.1"
                     /translation="MRVVQSSFEDLGAPLSQVTFCFVDLETTGGSPADSRITEIGAVT
                     FRGGERLGSFQTLVDPLVPIPRFITHLTGIDDLEVRGAPPIEAALPSFLEFARGTVLV
                     AHNASFDYRFLCAEADRLDYERPPGPPVCTAKLARRVLRNEVPNARLATLADYFRTVT
                     RPQHRALADAEACAEVFHGLLDLGGRLGILTLSDLRGAMRARGTPHWQKASMADGLPG
                     GPGVYLFRSKDGEVLYVGKAKDVRARVRSYFYGDDRKRIDDLLASAEEVEAIPCRTEV
                     EALVLEARLIRQHSPPYNRRGKAWRRACYLKLDLDEAWPRLKVVRKVSGDGAYLGPFG
                     SSKQATLAKEALEEIVPIRRCTRSMGRSTRFSPCALADLGRCVAPCDGRVAPERYEEL
                     VRSLHSFLLAPGGLLAALEGRMEALGAQERFEEAALARDRLRALVTALARSRIERWLV
                     EAGRLEVESEEGLRLAFREGALVRRGDERGFTLPVPLDAAQEVRATTSWLAKARLRLV
                     DAEEAPREPVDGGAAIARLARRLATASR"
     gene            complement(1736..2440)
                     /locus_tag="VF097_00795"
     CDS             complement(1736..2440)
                     /locus_tag="VF097_00795"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX6261362.1"
                     /translation="MGELDAPLLVDFPLRGEWTVERTPADRIPSHGTDAFGLRYAYDF
                     IRTDQRRGLHPHPSGTARWILVGGRTQDCYGWDQPVHAAAGGQVVEAVDGVPERRWIH
                     PVVEALAALRNTVALVRGRLDLTRLAGNHVIVESKSVYALYGHLAPGSVTVTKGGAIP
                     AGHVIGRVGHTGNSTAPHLHFQLMDRADALTARGIPCAFEAYQVLRGERWMLVKRGVP
                     ERLERIRPVPRVNEGA"
     gene            complement(2447..3268)
                     /locus_tag="VF097_00800"
                     /pseudo
     CDS             complement(2447..3268)
                     /locus_tag="VF097_00800"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(3282..4226)
                     /locus_tag="VF097_00805"
     CDS             complement(3282..4226)
                     /locus_tag="VF097_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020518192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6261363.1"
                     /translation="MAAVPDPHPTSNGDPLIRSTGLTKTYGEVTAVRDLDLAVGRGEV
                     YGFLGPNGAGKTTTLRMLLGLIHPTSGSAEVLGRPPGDPEGLVRVGAMVEEPAFYPYL
                     TGRDNLRVITRYAGLSEDGIDEALGTVELLPRADSKFKTYSQGMKQRLGIAAALLKGP
                     DLLILDEPTNGLDPAGMVEMRTLIRHLGTGERTVLLSSHLLAEVQQICDRVGVISKGR
                     LVFEGSVDELRGRAAVAIRAEPLDLARRRAEEVLGPGRVEVRDGELVVDAEPAEVPAL
                     NRELVLADVQVSELRPASRSLEEAFLQLTGEGGDDDEG"
     gene            4300..4503
                     /locus_tag="VF097_00810"
     CDS             4300..4503
                     /locus_tag="VF097_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018557044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261364.1"
                     /translation="MATRFRCIPCGNLTRFDVVQSRRTKAYYHFALSGELEIEDEEVL
                     ASERESVTCRWCGSTEVQEEEVT"
     gene            4500..5639
                     /locus_tag="VF097_00815"
     CDS             4500..5639
                     /locus_tag="VF097_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX6261365.1"
                     /translation="MSGPLEGIRILDLTRLLPGNHCTLLLADLGADVVKVEEPGRGDY
                     VRWFEPLVDGVSAIHRAVNRGKRSVALDLKRAEGVDAFRRLASGADALIESFRPGVMD
                     RLGVGYEALSAENPRLVWCSISGFGQDGPYRDRVGHDLNYLGYAGVLHAAGPPDGVPW
                     PPPVQPSDLAAGMAGALGVVASLMDARQTGRGRFVDASMLDAAVSWGVMGMAVLLGSG
                     SPPPRGGTMLTGGLACYRVYRCADDGLLSVAALEERFWRNLCEALEAPDLLEGHLDPA
                     EQERLAGRLEEIFGMRPRDEWVDRLAELETCVGPVLDMSEAMENPQVRHRGLAEVEGE
                     VVGPGPTLRVSGAEADLRPAPGLGEHTEEVLREVGLSEEELAALR"
     gene            5714..5944
                     /locus_tag="VF097_00820"
     CDS             5714..5944
                     /locus_tag="VF097_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261366.1"
                     /translation="MRIDCERCPYRRGSLRCADCLVEFVLRLEDGDVVVEAEEEVDGT
                     VELDAEEARAIRALSDAGLIEPVRIDPLEDAG"
     gene            6024..7331
                     /gene="sepH"
                     /locus_tag="VF097_00825"
     CDS             6024..7331
                     /gene="sepH"
                     /locus_tag="VF097_00825"
                     /inference="COORDINATES: protein motif:HMM:NF040712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein SepH"
                     /protein_id="HEX6261367.1"
                     /translation="MKRIYLVGASDDKKRLQLAQGKGARFGSMELPISPKLRALLREV
                     EAERLAKRGKKPPEEELKEQRTRAEQAKAEAKRAREGLEAASRAAREAEKRAREAEKR
                     AAQAEKRAQRAEEGATEARRRAEEAKAKAEAAEKRAGEVADRVRERSPKPDGGPPAKG
                     GLAPGGFERATGGMEGVHAIIRPTSVATSRAAPGPKTPARRRAPARATAEKPAEERSK
                     EEGSKDERPRKEAAPRRKAPPMPEPKLTPAEIQTLLRSGRGIRSVAKQAEAPVDWVRR
                     LAAPIESERLGVVTQLMQSYVVRARLGRSAVPVGTAIVENLRDRGVRFPERVAEDGWT
                     AWRPDNREWRVRFTYESRGRRQRADWRFEPQSRTVVPENDLAGELAYRAPAEGDGVGG
                     AGAALRPQRVSGGSRAAPAPPTKKSTSKKGSRGRASRKRSAGR"
     gene            complement(7317..8165)
                     /locus_tag="VF097_00830"
     CDS             complement(7317..8165)
                     /locus_tag="VF097_00830"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261368.1"
                     /translation="MGRATDLSAVLEGRAYAQLLGWRVIEVSGADARGWLHDLLMADL
                     VSLSTARARRALMLKPTGHIRADLTVASRGDGFLLVQDPRQPTPIDLLLTPYVLSADV
                     RLEDRTGELRVVAFPERPAESADAYAPSATGPGHDVILPREAVVEARGAEEVGLEAIE
                     AWRIARGEARFPQDLGERSLPNEAGLEALIEDAEKGCFLGQEAVAKTRTLGRPPFAVI
                     RARADRPVEEGQAVRAGDDDVGEVTSAAEVDGGWALIARVRWSSREGVLTANGRAIEV
                     RGLATG"
     gene            8186..9334
                     /locus_tag="VF097_00835"
     CDS             8186..9334
                     /locus_tag="VF097_00835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6261369.1"
                     /translation="MDAAADGPEHLLPRILVVTNDFPPRVGGTQRYVHDLVRHLPPDQ
                     VTVLAPPWEGWEAFDQAQPFPVLRHRRMLAPTPELRRRVLSLVDETGAEIVLFGHGYP
                     LGELGPGVKAATGVPYAMLTHGLEFMLAAMPVVRSRMRRAFSEARVVFTVSRFMQHKL
                     RRAIPWQVPRMPLPPGVDLERFHPGVSGLEVRARHGLSDRLVVACISRLVPRKGQDVL
                     LRAWPRVLRHVPDAALMLVGDGPHRGALARLRSRVDGTVVFAGEVPEEELPAYYAACD
                     VFAMPCRDERFGFEVEGLGIVYLEAAASARPVIAGRSGGAPEAVLDGETGFVVDGADV
                     ADVTEALVALLRDPSEARRLGKAGRARAEREWGWDVVIRRFASAIAPR"
     gene            complement(9331..10311)
                     /locus_tag="VF097_00840"
     CDS             complement(9331..10311)
                     /locus_tag="VF097_00840"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6261370.1"
                     /translation="MSLEWIADPGVFELDDWERLRAADPEATFFQTPRFLKIYREQLG
                     EGTPLVGLVREDERAIGLCPFEISGGLLSFLGGFEVTDYLGPVGRPEDRARVAEELMT
                     GLAAREDWDRGDLEGLPDDGMWLGALADAAEAAGLRTEIGDDDVAPMLELPPTWDEYL
                     TELKPKQRHEIRRKDRRFRERFAEVRLRASNHGTLERDLDRFVEMHRASEGPKGTFME
                     EAMVGFFRRLGQELLPTGVFRLTFLEGDGEAVAGLASFRFEGEMLLYNSAFDPRHRAV
                     APGMVLVAETIREAIAEGCARLDMLKGDLGYKYRFGARPRAIRRLRLARR"
     gene            complement(10343..10906)
                     /locus_tag="VF097_00845"
     CDS             complement(10343..10906)
                     /locus_tag="VF097_00845"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6261371.1"
                     /translation="MFLRENEIGRRIAPWVEPGMRLLDVGSGTGRIARWLARERRALP
                     TTSDLHEFGNRVGDLPFRQITDPVRVPADDDEFEAVLLLFVLHHMPVWEDQIRLLADA
                     ARAARRRLIVIEDTPLHQVDRAFNVAWDWLLNLRHGVPKPFTFRDVEGWRRVFAGLGL
                     HERHAETYRPLWPTLGTYHHTLFVLET"
     gene            10986..11789
                     /locus_tag="VF097_00850"
     CDS             10986..11789
                     /locus_tag="VF097_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261372.1"
                     /translation="MSERCPRCGGLNAPDAEWCGQCFARLGEPEEPAVDPVVDPVEAA
                     AMLGELASTEGRTAEPAPGAPSDEAGGEPAGTSPGGPPADERSVEWEPSLQPGPATEA
                     SPFQVRENGLVWVCRLCEAENPLEAHNCARCGSPFGSLFEEERGAPEVDPGRASALSL
                     IFPGLGHLHAGHKVEGLARILIFVWLLGTIVVIVAARAGRGLGPLLGLVVLYMASAGA
                     LYMLSAIDARRAAEGEDPIVSSRILLYGISALLLVTIVFLFFTGPTLGG"
     gene            11824..12261
                     /locus_tag="VF097_00855"
     CDS             11824..12261
                     /locus_tag="VF097_00855"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6261373.1"
                     /translation="MGDRTEGDIQIEAEPEDVMEVLVDFDAYPEWAGVESAEILEEDD
                     EGRPAAVAFRLSAMGLDASYTLEYAYAPDDGGMSWTTREAGGALKDVAGEYVLEASDD
                     GTHVTYRLAVELAVAVPSLLRKQGEKRIVRTALEGLKRRVEEG"
     gene            12308..13504
                     /locus_tag="VF097_00860"
     CDS             12308..13504
                     /locus_tag="VF097_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HEX6261374.1"
                     /translation="MRILLFTGKGGVGKTTIAAATAVRAARSGQRTLITSTDPAHSLA
                     DAFDVEIGEAPLQVEDRLWAEQVNAQARLESNWRDIQEYIIQFLNWAGVDAIEAEELS
                     VIPGLDEIFSLTDMKRHVDAADYDLLVIDCAPTAETLRLLSLPEVMNWYIERIFPVER
                     RVVKTIRPILTRLTTMPIAEDRIFGAIEQLHRNLEGVRAILGDAERSSVRLVVNPEKM
                     VIAEARRTYTYLSLFGYRVDAIVANRIIPEEVNDPYFGKWKDIQAEHLATIHESFQPI
                     PILTSRLFGQEMVGPDLLEDMGNEVYGEGDPAAVLYTEEPMRIRRRGPLYLLTLRLPF
                     VDRGSLDIHRKGEELFVRVGSHKRNLILPQALQRLDVRDAQLVDDRLEIAFGEEAQSR
                     AGAGGG"
     gene            13522..13752
                     /locus_tag="VF097_00865"
     CDS             13522..13752
                     /locus_tag="VF097_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261375.1"
                     /translation="MAEKETARDEGTDVPTCPVSICPVGMMLTLAGQAKPEVVEHLLN
                     AGRELIMAATVFLNARADAVSESPRLERIDVD"
     gene            13769..14890
                     /locus_tag="VF097_00870"
     CDS             13769..14890
                     /locus_tag="VF097_00870"
                     /inference="COORDINATES: protein motif:HMM:NF037429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vms1/Ankzf1 family peptidyl-tRNA hydrolase"
                     /protein_id="HEX6261376.1"
                     /translation="MATLDQGLLRKLSDWSPAHPVTSLYLDVDGRRHPSRNGYLVRMR
                     DLFKGMSLDGLDREQRRSVAEDMEAIRRHVEDELDRSRVGGVAVFSSSRSGLWESVPV
                     SRPFRDRLHVGSRPLLIPLEGVLERFETFCVLVADREHAKLLYVRQDLVEELKEIHDD
                     VPGRHEQGGWSQARFQRHIEDHVLRHLKHTAQVALRAAQRRPFDHLILAGSEELVAEL
                     ERELHDYVARKVLDRIVLPITVSAHEVRERVSAIEAELESRRESEALERLTIEARAGT
                     GRAVAGLAGTLEALESGRVETLVVAADLRADGRRCARCGHLSLAEVRCAACGADTEAA
                     DHIVEEAVEIAVRQKCRVETIGGAEELAALGGVGALLRF"
     gene            14894..15847
                     /locus_tag="VF097_00875"
     CDS             14894..15847
                     /locus_tag="VF097_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006544794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX6261377.1"
                     /translation="MGERPLAIGLDVGGSKVSGVLMSDEGSVLASELLASPADDVEAM
                     VETVVLAAERLRAKGPAAAVGLGAAGMVEHTTGVLRSAPNIAWTEVPLRDLVRRRIGL
                     PCVVDNDANAAAWGEFRFGAGRGLRHVLLVTVGTGIGGGLIQNGRLYRGAHGFAAEIG
                     HIIVEPDGPPCGCGNRGCWEQMASGQALERMAREAALGDPAGPIARTAGGRPPVGPDV
                     VAAAAAGDPAAGTILETVGRRLGEGLAGLANVLDPEAIVVGGGMAEIGAPFLDPARVR
                     FLEAVEAPDHRPEIPILQAVLGNDAGAIGAAAMALEPLEDG"
     gene            15844..16698
                     /locus_tag="VF097_00880"
     CDS             15844..16698
                     /locus_tag="VF097_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6261378.1"
                     /translation="MKTGVVLPLFSGNPEKVLAAARETEALGYDGAFVFDHFFPPGAS
                     SDRPSLEAFTTLAAVAAVTERIRVGTLVTRASLRPPGILAKQASWIDAASDGRLILGI
                     GTGDETDGDEHRAFGLPLMGRDERRAHLEETATTLRALFEGRDGALEGPLAPSASPPI
                     WIGGLADAIVRIAARHADGWNGWGLDVPTFARKAGLLREGAGDRGVEATWAGIVLVGR
                     DAAELAELKARRADRGMEDASWSGTAAELVGFLTELGRAGAGWAIMVAAGPRDRRELI
                     AEEVLPEL"
     gene            16754..18079
                     /locus_tag="VF097_00885"
     CDS             16754..18079
                     /locus_tag="VF097_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HEX6261379.1"
                     /translation="MAKRVVIMGAAGRDFHNFNVVYRGDREVEVVAFTATQIPGIENR
                     RYPPELAGDRYPEGIPIRLEDELADLIARERVDEVVFAYSDVSHEYVMHRASAVLAAG
                     ATFTLLGPEATMLDSTKPVVAVCAVRTGAGKSQTTRAVVRALKAQGRKPGVVRHPMPY
                     GDIVAQRVQRFATMDDLDAADVTVEEREEYEPHIASDTVVYAGVDYAEILEAVQQEAD
                     VVIWDGGNNDLPFYRPDVHLVVADPHRPGHETRYHPGEANLRMGDVIVINKVDTAEPE
                     NVDAVEATARELNPDAVIIRANSPVTVDDPGAVEGARVLVIEDGPTLSHGEMTYGAGV
                     VAARQHGAAEIVDPREWAVGSLKTVYEKYPVGPVLPAMGYSDEQIRELEETIDAVPCD
                     LVMVATPVDLSRIASFSKPSVRVGYELEEGEGQPTIAEVLAARLPAAGG"
     gene            18081..19013
                     /gene="arcC"
                     /locus_tag="VF097_00890"
     CDS             18081..19013
                     /gene="arcC"
                     /locus_tag="VF097_00890"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014013167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEX6261380.1"
                     /translation="MPRVVIALGGNALLQREEEDTAQVMVRNARTAAEQVADVAASGW
                     EILVTHGNGPQVGRILLQNEAARDWAHPMPLDVCGAMSQGQIGYLLQVTIGDVFFERG
                     MERPVTTLLTLTRVRADDPAFQGPTKPIGPFYEEAEAARLAEERGWVVKEVGEGGWRR
                     VVPSPRPYSIVEAPIARQLAESETIVICTGGGGVPVIEEGPRLVGVEAVVDKDHASAI
                     MAREVEAQVLMILTDVEGLYERFGEPDQRLLERLTVDDAERVLDSLPAGSMGPKLEAC
                     VDFVRGGGERAVIGPLDDALAALEGRTGTSIVST"
     gene            19036..19707
                     /locus_tag="VF097_00895"
     CDS             19036..19707
                     /locus_tag="VF097_00895"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX6261381.1"
                     /translation="MDLRVVVWNAHGLRAGVAAAAEALARLRPDLVMLNEVGWSGWRL
                     ARLARALDARAASGLRRLRWSVTNAVLVLPPWRLLRTRTIRLPGRSRPHRGVVASLIG
                     RSGYRLTAASVHLGLSDPERREHARDLTDRILPELRPPVLLGGDLNEPPEGPAARWMA
                     ERLWDAFAQAGQGPGETFPARDPRARIDYLLVPEGVTVARAFVDRDAAEASDHLPLVV
                     DLELA"
     gene            complement(19704..20453)
                     /locus_tag="VF097_00900"
     CDS             complement(19704..20453)
                     /locus_tag="VF097_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6261382.1"
                     /translation="MSAEVVPGAEPFSFPGGDARVLMLHGFTGNPSSLRPWGEALAER
                     GFTVCGPRLPGHGTTWQDLATRRGDEWVAAADAALTKLRGDGGRVVICAQSFGGTIAL
                     DLASRRTGDVRGIVLVNPYVRDPRHVWLPVVRPFLRTIKGIGNDIKKPGGDELPYPRV
                     PLRAVSEVAGMMKRLEAAIPSIVQPLLLFHSPEDHVVPRGTAEWLLDRIGSEDKELVL
                     LQDSYHVAALDNDAPTIFQRSAAFIERVTRG"
     gene            complement(20450..20818)
                     /locus_tag="VF097_00905"
     CDS             complement(20450..20818)
                     /locus_tag="VF097_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6261383.1"
                     /translation="MAPRKTILLVEDDDSVRQLVRVSLELHDFDVLEARGGAEGLELL
                     ERHRPDAVVLDLMMPGVGGESLLTQLRAAPATRGTPVVVVSGKPEVGHDVLGLVGREN
                     FIVKPFDPEALIDRVRAAVG"
     gene            complement(20823..20972)
                     /locus_tag="VF097_00910"
     CDS             complement(20823..20972)
                     /locus_tag="VF097_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261384.1"
                     /translation="MESGWGVLEWTFVIVVGSLSLAAGLFGLYLLALLFRNTGLRRRP
                     NPRGR"
     gene            21053..21787
                     /locus_tag="VF097_00915"
     CDS             21053..21787
                     /locus_tag="VF097_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX6261385.1"
                     /translation="MFYWFLKYVLLGPLLRLIYRPKAVGLEHVPAEGPVILASNHLSF
                     VDSLFIPLLVKRPVVFLGKADYFDSWRTRWFMKAVRVIPVRRTGGSASEAAIRTGVEA
                     LGEGLVIGIYPEGTRSPDGRLYRGKTGVARMALEAQTPVVPIAVDGTVDIMPLDKKMP
                     RLRGRVTVRFGPPLDFARYYDRPRDRFVLRSVTDEIMYEIMMLSGQEYVDEYAAKVKK
                     ELAAQERTVRVPDTETVDQDSRTGAA"
     gene            complement(21788..23281)
                     /gene="glpK"
                     /locus_tag="VF097_00920"
     CDS             complement(21788..23281)
                     /gene="glpK"
                     /locus_tag="VF097_00920"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEX6261386.1"
                     /translation="MSPVARILAIDAGTSGVKALIVNEAGSVLGTGYREFAQSFPRPG
                     WVEHEPDDWWAATQEATLAALRAADLRASDLTALGITNQRETTVLWDRETLHPVHPAI
                     VWQDRRTAPMCAELLDEGWEERVIERTGLVIDPYFSGTKLAWLLDQVDGAREAAEAGR
                     LAFGTVDAYLISRLTGGRAHVTDHTNASRTMLFDILRLDWDPELLERLRIPEAVLPEV
                     GPSSGSFGETDPDAFLGIRVPIAGVAGDQQAALVGQACFTPGQAKNTYGTGSFVLLNT
                     GDHAPRAKGLLTTIAYSDEEGVRYALEGSIFVTGAAIQWLRDGLGIIRAAAEIGPLAA
                     SVPDTEGVYFVPALTGLGAPWWDPHARGTIVGITRGTNRAHLARAAVESMAFQTRDVV
                     DRMVADSEVGLSQLKVDGGASVMDLLCRFQADLLGVPVRRPKVQETTSLGAAYLAGLS
                     TGVWSSKDEAARAWELDAEFLPSGEDLTERYDDWRRAVERSLAWARG"
     gene            complement(23306..>23721)
                     /locus_tag="VF097_00925"
     CDS             complement(23306..>23721)
                     /locus_tag="VF097_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008286579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX6261387.1"
                     /translation="AERRARARAPLITGGLDQSELLALLGPDGGRGCDIPSGLRGTGS
                     GFEGIASWYGWDFAGRPTASGAIFDPRLFTAAHRTLPLPSFLHVRHGGRCATVLVNDR
                     GPFIEGRVLDLSQASAEYLGVSLSHVEAEILVPRG"
BASE COUNT         3344 a         8235 c         8969 g         3173 t
ORIGIN      
        1 gggtccgcgc gccctcgacg atgacggctt gctccacggg gtcaatccta ccagcggcct
       61 ctaccgcgag gccgtggcga gacgccgagc aagcctggcg atggcggcgc ccccgtcgac
      121 gggctcccgc ggcgcctcct cggcgtcgac cagcctaagc cgggccttcg ccagccagct
      181 ggtcgtcgcc cggacctcct gggccgcgtc gagcggcacg ggtaaggtga acccccgttc
      241 gtcgccccgc cgcaccagcg ccccttcccg gaacgccagc cgcagaccct cctcggactc
      301 cacctccaac cggcccgcct cgaccagcca ccgctcgatc ctcgacctgg ccaacgcggt
      361 cacgagcgcc cgcagtcggt cccgggccag cgcggcctcc tcgaaccgct cctgggcccc
      421 gagcgcctcc atccgcccct cgagcgccgc gaggagcccg cccggtgcga ggaggaagga
      481 gtgcagcgac cgaacgagct cctcgtagcg ctcgggggcg acccggccgt cgcagggcgc
      541 gacgcagcgg ccgagatccg cgagcgcgca cggcgagaac cgggtggagc gccccatgga
      601 gcgggtgcag cggcggatcg ggacgatctc ctcgagggcc tccttggcca gcgtggcctg
      661 cttgctgctg ccgaacggac cgaggtacgc gccgtcgccg ctcaccttcc gcaccacctt
      721 gaggcgcggc caggcctcgt cgaggtccag cttgaggtag caggcccgcc gccacgcctt
      781 cccccttcgg ttgtaaggag gggagtgctg ccggatcagc ctcgcctcga gcaccagcgc
      841 ctcgacctcg gtccggcagg ggatcgcctc gacctcctcg gcgctcgcca gcaggtcgtc
      901 gatccgcttg cggtcgtccc cgtagaagta ggagcgcacg cgggcccgca cgtccttggc
      961 cttgccgacg tagaggactt cgccgtcctt gctccggaag aggtacacgc ccgggccgcc
     1021 cgggaggccg tcggccatcg aggccttctg ccagtgcggc gtgccccgcg ctcgcatcgc
     1081 cccccggagg tccgagagcg tgaggatgcc caggcgcccg ccgaggtcga ggaggccgtg
     1141 gaagacctcg gcgcaggcct cggcgtcggc gagcgcccgg tgctgggggc gggtgacggt
     1201 gcggaagtag tcggccagcg tggcgagcct cgcgttcggt acctcgttcc ggaggacgcg
     1261 gcgggccagc ttcgcggtgc agacgggcgg cccgggcggc cgctcgtagt cgaggcggtc
     1321 ggcctccgcg cagaggaacc gatagtcgaa ggaggcgttg tgtgccacga gcaccgtccc
     1381 tcgggcgaac tcgaggaacg aggggagcgc ggcctcgatc ggcggcgcgc cccggacctc
     1441 caggtcgtcg atccccgtga ggtgcgtgat gaagcgaggg atgggaacca gcgggtccac
     1501 cagcgtctgg aaggatccga ggcgctcacc gccccggaag gtgacggccc cgatctcggt
     1561 gatgcgggag tcggcgggcg agccgcccgt cgtctcgagg tccacgaagc agaacgtgac
     1621 ctgggagagg ggagccccga ggtcctcgaa cgacgactgg acgacccgca cgcctcctcc
     1681 gatccgatcg gatgggcgcg cgcagacgag accatacccg aacacgtgtt cgaggtcaag
     1741 caccctcgtt gacgcgaggg acgggacgga tgcgctccag ccgctccggc acgccgcgct
     1801 tcaccagcat ccagcgttcg ccgcgaagca cctggtaagc ctcgaaggcg caagggatgc
     1861 ccctggcggt gagcgcgtcg gcccggtcca tcagctggaa gtgcaggtgc ggagccgtcg
     1921 agttcccggt gtgcccgacg cggccgatga cgtgtccggc ggggatcgcc ccacctttgg
     1981 tcaccgtgac gcttcccggc gcgaggtggc cgtacagcgc gtagacgctc ttggactcga
     2041 cgatcacgtg gttccccgcg agccgggtga ggtcgagccg gcctcggacg agagccaccg
     2101 tgttcctcag ggccgccagc gcctcgacca cggggtggat ccatcggcgc tccggcacgc
     2161 cgtccaccgc ctcgacgacc tgccccccgg cggcggcgtg aaccggctgg tcccatccgt
     2221 agcagtcttg ggttcgccct ccgaccagga tccaacgcgc ggttcccgaa ggatggggat
     2281 gaagaccgcg cctctggtcc gtccggatga agtcgtaggc gtagcggagg ccgaacgcgt
     2341 cggtcccgtg gctcgggatg cggtccgcag gggttcgttc gacggtccac tcgccgcgca
     2401 gtgggaagtc gacgaggagc ggcgcgtcga gctcacccac ggcggctcag gcgatgtcct
     2461 gacggcggag gacgatcgtg gacaggagcg ccatccccgc cacgtaggcg atgagaagcg
     2521 ccgtggcctc gggcccgtct gcgaccgtgg tcactccggg aacgcccccc gcctgatcct
     2581 gggcccgagc gccgagcgac gctgcgagcg cgcccgcgtt cacgcccggc agccactgct
     2641 ggatcgtcgc caggacctcc agcaggttgg cgaagccgct gatgaggctc tcgatcacga
     2701 gcgcccagac gaggcccaga ccgatcgcca gcgccgtgct ccggaacagg atgccgaggg
     2761 tcatcccgaa caggctccac atcccgagga tcagccagcc cgcaccgacc ccttggagca
     2821 gctcgacgat cccgggccac gagacggccc ggttctctgc caccgcgatc gtccagctcg
     2881 ccccggcgct caggagaaag aagacgacgg tgatcgccag cgtgacgagg gcgatcgcga
     2941 gcagcttccc cgccaggacc tccacccgac gcgggcgctg gacgaggatc gtcttgagcg
     3001 tcccccagcc gtactcgctg ctggccacca gggcgccgat gatcagggcg atggccccgc
     3061 cgaacagcgg gaagccgccg accacgctcg ggacgaggtt gtgcggcagc gcctcgttca
     3121 ggatctgttc cacgggcacg ccctcgagct cggggccgcc tccggtcttg aagatgaggt
     3181 aggggaccac gtagccgaag aagaggccca tcacgaagta gatgccgacg aggacccaca
     3241 tcgcgggccg cttccgcagc ttcaggagct cggcggcgag gctacccttc gtcgtcgtcg
     3301 cccccttccc cggtgagctg gaggaaggcc tcctccaggg acctggacgc gggccgcagc
     3361 tcggacacct gcacgtcggc cagcacgagc tcccggttca gcgccggaac ctcggctggt
     3421 tcggcgtcca cgacgagctc gccgtcgcgg acctcgacgc ggcccgggcc caggacctcc
     3481 tccgcgcgcc gtcgggccag gtcgaggggc tcggcccgga tcgcgaccgc ggcgcgtccc
     3541 cgcagctcgt ccaccgagcc ctcgaagacg agccgcccct tggagatcac ccccacgcga
     3601 tcgcagatct gctggacctc ggccaggagg tggctggaga ggaggacggt gcgctcgccg
     3661 gtgcccaggt ggcggatcag cgtccgcatc tcgaccatcc cggcgggatc gaggccgttc
     3721 gtgggctcgt ccaggatcag caggtcggga cccttgagga gcgcggccgc gatcccgagc
     3781 cgctgcttca ttccctgcga gtaggtcttg aacttgctgt cggcccgggg cagcagctcc
     3841 acggtcccga gggcctcgtc gatcccgtcc tcggagagcc ccgcgtatcg ggtgatgacc
     3901 cgaaggttgt cgcggccggt caggtaggga tagaaggccg gctcctcgac catggccccc
     3961 acccggacga ggccctcggg atcgcccggc gggcggccca ggacctcggc ggagcccgag
     4021 gtgggatgga tcagccccag cagcatccga agggtggtgg tcttccccgc gccgttcggt
     4081 ccgaggaagc cgtagacctc gccgcgcccg accgcgaggt cgaggtcgcg gaccgcggtc
     4141 acctccccgt aggtcttcgt cagcccggtc gagcggatga gtgggtcacc gttcgatgtg
     4201 ggatgaggat cgggcacggc ggccatcgcc cacgagccta cccctcgccg gcgcgaccca
     4261 tcaggctcgg gggatcgctc cggtacgatg gcctccgcta tggcgacgcg attccgctgc
     4321 atcccgtgcg gcaacctcac gcgcttcgac gtcgtgcagt cgcggcggac gaaggcgtac
     4381 taccacttcg cgctgtcggg cgagctagag atcgaggacg aggaggtgct ggcctcggag
     4441 cgcgagtcgg tgacgtgccg atggtgtggg tcgaccgagg tccaggagga ggaggtgacg
     4501 tgagcgggcc gctcgagggg atccgcatcc tcgacctgac gcggctgctg cccggcaacc
     4561 actgcacgct cctgctggcc gatctggggg cggacgtggt gaaggtcgag gagccgggcc
     4621 ggggagacta cgtgcgctgg ttcgagccgc tcgtggacgg ggtgagcgcc atccaccggg
     4681 cggtgaaccg ggggaagcgt tcggtggctc tcgacctgaa gcgggccgaa ggcgtggatg
     4741 cgttccggcg gctcgcatcg ggtgcagatg cgctgatcga gtcgtttcgc cccggggtga
     4801 tggatcggct gggtgtgggc tacgaggcgc tctcggcgga gaacccgcgt ctcgtctggt
     4861 gctcgatcag cggcttcggg caggacgggc cgtaccgcga ccgggtgggc cacgacctca
     4921 actacctcgg ctacgcgggg gtcctgcacg ccgcggggcc gccggacggg gtcccgtggc
     4981 cccctcccgt ccagccctcg gatctcgcgg cggggatggc gggggcgctg ggggtcgtgg
     5041 cctcgctgat ggacgcgcgg cagacgggca gggggcggtt cgtggacgca tcgatgctgg
     5101 acgccgcggt gtcgtggggc gtgatgggca tggccgtgct gctgggctcc gggagcccgc
     5161 cgccccgcgg agggacgatg ctgacgggcg ggctggcctg ctaccgcgtg taccggtgcg
     5221 cggacgacgg gctgctgtcg gtggctgcgc tggaggagcg cttctggcgg aacctgtgcg
     5281 aggcgctgga ggcgccggat ctgctcgagg gccacctcga ccccgcggag caggagcggc
     5341 tggccggtcg tctcgaggag atcttcggga tgcggcctcg ggacgagtgg gtggaccggc
     5401 tcgcggagct cgagacgtgt gtcggtccgg tgctcgacat gtcggaggcg atggagaacc
     5461 cgcaggtccg gcaccgaggg ctggccgagg tggagggcga ggtcgtgggg cccgggccca
     5521 cgctacgggt ctcaggggcg gaggcggacc ttcggccggc gcccggcctc ggggagcaca
     5581 ccgaagaagt cctccgggag gtgggcctca gcgaggagga gctcgccgcg ctgcgctgag
     5641 cgtccgtgtc agagggggcc ggtaccgtgg tgctcgaacg gccccccacg acagcggacc
     5701 gagaggagac gccgtgcgga tcgattgcga gcgatgcccc taccggcgcg gcagcctgcg
     5761 ctgcgccgac tgcctggtcg agttcgtctt gcgcctcgag gacggcgacg tcgtggtcga
     5821 ggccgaggaa gaggtggacg ggacggtcga gctggacgcg gaggaggccc gggcgatccg
     5881 ggcgctgtcc gacgcgggcc tgatcgagcc ggtgaggatc gacccgctcg aggacgccgg
     5941 ctgaggggag gccgccggag agttcgttgc cccgctcctg gtgaggggcc acaatgccct
     6001 gaacggccag ttcaggggat gccttgaagc ggatctacct cgtcggcgcg tccgacgaca
     6061 agaagcggct ccagctggcg cagggcaagg gggcgaggtt cgggagcatg gagctcccca
     6121 tcagccccaa gctccgcgct ctccttcgcg aggtcgaggc ggagcgcctg gccaagcggg
     6181 ggaagaagcc gcccgaggag gagctgaagg agcagagaac ccgggccgag caggcgaagg
     6241 ccgaggccaa gcgggcccgc gaggggctgg aggccgcctc ccgggcggcc cgggaagccg
     6301 agaagcgggc gcgggaagcc gagaagcgcg ccgcccaggc cgagaagcgg gcccagcggg
     6361 cggaagaggg ggcgaccgag gcgcggcggc gggccgagga ggcaaaggcg aaggcggagg
     6421 ccgcggagaa gcgcgccggg gaggtggcgg accgggtccg tgagcggtcc cccaagcccg
     6481 acggggggcc accggcaaag ggcggcttgg ctcccggcgg cttcgagcgc gccaccggcg
     6541 gcatggaggg ggtccacgcg atcatccggc ccacgtcggt ggcgacgtcc cgcgccgcgc
     6601 cagggcccaa gacgcccgcg cgccgacgcg ccccggcccg cgcgacggca gagaagcccg
     6661 ccgaggagcg ctccaaggag gagggctcca aggacgagcg ccccaggaag gaggccgctc
     6721 cccggaggaa ggcgccgccc atgcccgagc cgaagctcac gcccgccgag attcagaccc
     6781 tcctgcgatc cggacggggg atccgttcgg tggccaagca ggccgaagct ccggtggact
     6841 gggtgcgccg gttggcggcc ccgatcgagt cggagcggct tggggtggtc acgcagctga
     6901 tgcagtccta cgtcgtgcgg gcccggctgg ggaggtcggc cgtgccggtc gggacggcga
     6961 tcgtggagaa cctgcgggat cgtggcgtgc ggttccccga gcgggtggcc gaggacgggt
     7021 ggactgcctg gcgccccgac aaccgcgagt ggcgggtccg gttcacctac gagtcccggg
     7081 ggaggaggca gcgggccgac tggcggttcg agccgcagtc gcggaccgtg gtcccggaga
     7141 acgacctggc cggagagctg gcgtaccggg cgccggccga gggcgacggg gtggggggcg
     7201 cgggcgcggc cctccggccc cagcgggtct cgggcgggag cagggccgcg cccgcgcccc
     7261 ccaccaagaa gtcgacctcc aagaagggct cgaggggtcg ggcctcgcgg aagcggtcag
     7321 ccggtcgcta ggccccggac ctcgatggcg cggccgttcg cggtgagtac cccctcgcgg
     7381 ctcgaccagc gcacccgcgc gatgagggcc catccgccgt cgacctccgc ggcgctcgtg
     7441 acctcgccca cgtcgtcgtc gccggcccgg accgcctgcc cctcctccac gggccggtcc
     7501 gcacgggccc ggatcacggc gaagggtggc ctccccagcg ttcgcgtctt ggccaccgcc
     7561 tcctgaccca ggaagcagcc cttctcggcg tcctcgatca gagcctccag cccggcctcg
     7621 ttggggaggg accgctcccc gaggtcctgc gggaagcgcg cctcgccccg cgcgatccgc
     7681 caggcttcga tcgcctcgag tccgacctcc tccgcccccc gcgcttcgac gaccgcctcg
     7741 cgggggagga tcacgtcgtg gcccgggccg gtcgcggagg gagcgtaggc gtccgcactc
     7801 tcggccggac gctccgggaa ggccacaacc cggagctcgc cggtccgatc ctcgaggcgg
     7861 acgtcggcgg agagcacgta gggggtgagg aggaggtcga tcggcgtggg ctggcggggg
     7921 tcctggacga ggaggaagcc gtcgccgcgg gaggccaccg tcaggtcggc ccggatgtgg
     7981 ccggtcggct tgagcatcag cgcccgccgg gcccgggccg tgctcagcga gacgagatcg
     8041 gccatgagga ggtcgtggag ccaccctcgg gcgtcggccc ccgagacctc gatgacgcgc
     8101 cagcccagga gctgcgcgta cgcgcgcccc tcgagcacgg cggacaggtc ggtcgctcgt
     8161 cccacgggtg caggataatc accaggtgga cgccgccgcc gacggccctg agcatctcct
     8221 cccacggatc ctggtcgtga ccaacgactt cccgccccgc gtgggcggga cgcagcggta
     8281 cgtccacgac ctcgtgcggc acctcccacc cgatcaggtg accgtcctgg cgcccccgtg
     8341 ggaggggtgg gaggcattcg accaggcgca gcctttcccc gtgctgcgcc accggaggat
     8401 gctggccccc acgcccgagc tccgccggcg ggtcctctcg ctcgtcgacg agaccggagc
     8461 cgagatcgtg ctgttcggcc acggctaccc gctgggcgag ctgggtcccg gcgtcaaggc
     8521 ggcgacgggc gtgccctatg cgatgctgac gcacggcctc gagttcatgc ttgcggcgat
     8581 gcccgtggtg cgctcgcgga tgcggcgcgc cttctcggag gcgcgggtgg tcttcaccgt
     8641 cagccggttc atgcagcaca agctccgtcg ggccatcccg tggcaggtgc cgcgcatgcc
     8701 gctccccccg ggggtggacc tggagcgctt ccaccccgga gtgagcgggc tcgaggtgcg
     8761 agcgcgacac gggctgagcg accgcctggt ggtggcgtgc atctcccggc tcgtccctcg
     8821 gaaggggcag gacgtgctgc ttcgggcatg gccccgcgtg ctgcgccacg tccccgatgc
     8881 cgcgctcatg ctcgtggggg acggaccgca tcgaggggcg ctggctcgcc tccggagcag
     8941 ggtggacggc acggtggtct tcgcggggga ggtgcccgag gaggagctcc cggcctacta
     9001 cgccgcctgc gacgtcttcg ccatgccctg ccgggacgag cgcttcggct tcgaggtgga
     9061 ggggctgggc atcgtgtacc tggaggcagc cgccagcgcg cgtcccgtga tcgccgggcg
     9121 gtcgggcggc gcgcccgagg cggttctcga cggggagacc ggcttcgtgg tggacggggc
     9181 cgacgtcgcg gacgtcaccg aggcgctcgt ggccctcctc cgggatccgt cggaggcccg
     9241 caggctgggc aaggccggcc gggctcgggc tgagcgggag tggggctggg acgtggtgat
     9301 ccggcggttc gcgagcgcga tcgccccccg ctagcgacgc gcgagacgga gccgccggat
     9361 ggccctgggc cgcgccccga accggtactt gtagccgagg tcccccttca gcatgtcgag
     9421 gcgggcgcag ccctcggcga tcgcctcccg gatggtctcc gccacgagca ccatcccggg
     9481 cgcgacggcg cggtgccggg gatcgaaggc cgagttgtag aggagcatct cgccctcgaa
     9541 ccggaaggac gccaggccgg ccacggcctc cccgtccccc tcgaggaagg tgagccggaa
     9601 cacgccggtg ggcagcagct cttgtccgag gcggcggaag aagcccacca tcgcctcctc
     9661 catgaaggtc cccttcggcc cctcgctggc ccggtgcatc tccacgaaac gatcgaggtc
     9721 ccgttccagg gtgccgtggt tggacgcccg gagccggacc tcggcgaacc gctcgcggaa
     9781 gcgccggtcc ttgcggcgga tctcgtgccg ctgcttgggc ttcagctcgg tcaggtactc
     9841 gtcccaggtc ggggggagct cgagcatcgg ggccacgtcg tcgtccccga tctcggtccg
     9901 cagcccggcc gcttccgcgg cgtcggccag cgctcccagc cacatgccgt cgtcggggag
     9961 gccctccagg tcgccccggt cccagtcctc gcgggcggcg agcccggtca tcagctcctc
    10021 ggccactcgg gcccggtcct cgggccggcc cacgggcccc aggtagtcgg tcacctcgaa
    10081 cccgcccagg aacgagagca ggccgccgga gatttcgaag gggcacaggc cgatcgcccg
    10141 ctcgtcctca cggaccagcc ccacgagggg cgtcccctcg cccagctgtt cccgatagat
    10201 cttcaggaac cggggcgtct ggaagaaggt ggcctcgggg tcggccgcgc ggagccgctc
    10261 ccagtcgtcc agctcgaaga cgcctggatc ggcgatccac tcgagggaca cgtccggcat
    10321 cgtaccaacg acccctcgga cctcaggtct cgagcacgaa gagcgtgtgg tggtaggtgc
    10381 cgagggtggg ccagagggga cgataggtct ccgcgtggcg ctcgtggagc cccaggccgg
    10441 cgaagaccct tctccacccc tcgacgtcgc ggaacgtgaa gggcttgggg acgccgtgcc
    10501 gcaggttcag gagccagtcc caggccacgt tgaaggcgcg gtcgacctgg tgcagcggtg
    10561 tgtcctcgat cacgatgagc cggcgcctcg ccgcgcgtgc cgcgtcggcg agcagccgga
    10621 tctggtcctc ccacacgggc atgtggtgga ggacgaagag cagcaggacg gcctcgaact
    10681 cgtcgtcgtc ggccgggacg cgaaccgggt cggtgatctg ccggaacggc agatcgccga
    10741 cccggttgcc gaactcgtgg agatcggagg tcgtggggag ggcccgtcgc tcgcgcgcca
    10801 gccacctcgc gatccggccg gtgccggagc ccacgtcgag cagccgcatc ccgggctcca
    10861 cccagggcgc gatccgccgc ccgatctcgt tctcgcggag gaacacgggg gcaagcgtag
    10921 cgtgcgaggg cttccacgac gggggcgcgg ggccgtggca cgatagccgg gatggaggcg
    10981 tcaggatgag cgagcgctgt ccccggtgcg gggggctgaa cgcgcccgac gcggagtggt
    11041 gcggccagtg cttcgctcgg ctcggggagc cggaggagcc ggcggtcgac ccggtggtcg
    11101 acccggtgga ggccgcggcg atgctcggcg agctggcctc gacggagggg cggacggccg
    11161 agcccgcgcc cggggcgccc tcggacgagg ccggcgggga gccggcgggg acctcgcccg
    11221 gcggacctcc tgccgacgag cggtcggtcg agtgggagcc gtccctccag ccgggccccg
    11281 ccacggaggc cagccccttc caggtgcggg agaacgggct cgtctgggtg tgccgcctgt
    11341 gcgaggcgga gaacccgctg gaggcccaca actgcgcgcg ctgtgggtcg cccttcgggt
    11401 cgctgttcga ggaggagagg ggcgccccgg aggtcgaccc tgggcgggcc agcgcgctgt
    11461 cgctgatctt cccggggctg gggcatctcc acgcgggcca caaggtcgaa gggctggccc
    11521 gcatcctgat cttcgtctgg ctcctcggga cgatcgtcgt gatcgtggcc gcccgcgccg
    11581 gccgaggcct cggtccgctc ctggggctcg tggtgctgta catggcctcg gccggcgccc
    11641 tgtacatgct ctcggcgatc gacgcgcgcc gggcggccga gggggaggac ccgatcgtct
    11701 cgtcgcggat cctcctctac gggatctcgg cgctcctgct ggtcacgatc gtcttcctct
    11761 tcttcacggg gccgaccctg ggcggctgag cgggtcgcgt cgcatccgtg cgacactcct
    11821 gccatggggg accggaccga gggcgacatc cagatcgagg cggagcccga agacgtgatg
    11881 gaggtcctcg tcgacttcga cgcctatccc gagtgggcgg gggtcgagtc ggcggagatc
    11941 ctcgaggagg acgacgaggg ccgtcccgcg gccgtggcct tccggctgtc ggcgatgggg
    12001 ctcgacgcga gctacacgct cgagtacgcg tacgcgccgg acgacggggg gatgtcgtgg
    12061 accacccgcg aggccggcgg ggcgctgaag gacgtggcgg gcgagtacgt cctcgaggcg
    12121 tcggacgacg ggacccacgt cacctatcgg ctcgccgtcg agctcgcggt ggccgtgccg
    12181 tccctgcttc ggaagcaggg cgagaagcgg atcgtcagga ccgcgctgga ggggctgaag
    12241 cgccgcgtgg aggaggggtg agcctcgctc gcccccatcc gctcccggcg gctaccatgg
    12301 acgccggatg aggatcctgc tgttcacggg caagggcggc gtcgggaaga cgaccatcgc
    12361 cgccgccacc gccgtgcggg cggcccgttc cggacagcgg acgctcatca ccagcaccga
    12421 ccccgcccac tccctggccg acgccttcga cgtggagatc ggcgaggcgc cgctccaggt
    12481 cgaggaccgc ctctgggccg agcaggtcaa cgcacaggcc cgcctggagt ccaactggcg
    12541 ggacatccag gagtacatca tccagttcct gaactgggcc ggggtggacg cgatcgaggc
    12601 cgaggagctc tcggtgatcc cgggtttgga cgagatcttc agcctgaccg atatgaagcg
    12661 gcacgtggac gccgccgact acgacctcct cgtgatcgac tgcgccccga ccgcagagac
    12721 gctccgcctc ctgtcgctgc ccgaggtcat gaactggtac atcgagcgga tcttccccgt
    12781 cgagcgccgc gtggtgaaga cgatccgccc gatcctgacc cggctcacga cgatgccgat
    12841 cgccgaggac cgcatcttcg gggcgatcga gcagctacac cgcaacctcg agggggtccg
    12901 cgccatcctg ggcgacgccg agcggtcgtc cgtgcggctc gtggtcaacc ccgagaagat
    12961 ggtgatcgcc gaggcccgcc gcacgtacac ctacctctcg ctcttcggct accgggtgga
    13021 cgcgatcgtg gcgaaccgca tcatccccga ggaagtgaac gacccctact tcgggaagtg
    13081 gaaggacatc caggccgagc acctggccac gatccacgag tcgttccagc cgatcccgat
    13141 cctgacctcg cggctgttcg gccaggagat ggtgggcccg gacctcctgg aggacatggg
    13201 gaacgaggtg tacggggagg gggacccggc cgccgtcctc tacacggagg agccgatgcg
    13261 gatccgccgg cggggccccc tatacctcct gaccctccgc ctgccgttcg tggaccgggg
    13321 gtcgctggac atccatcgga agggggagga gctgttcgtg cgggtaggtt cccacaagcg
    13381 gaacctgatc ctgccgcagg cgcttcagcg gctcgacgtc cgagacgccc agctcgtgga
    13441 cgaccgcctc gagatcgcgt tcggggagga agcgcagagc cgcgccggcg ccggcggcgg
    13501 ctgaccgacg gagggggacc gatggcagag aaggagaccg cgcgggacga ggggacggac
    13561 gtcccgacct gtccggtgtc gatctgtccg gtggggatga tgctcacgct ggccgggcag
    13621 gccaagccgg aggtggtgga gcacctgctc aacgcggggc gggagctgat catggcggcc
    13681 accgtcttcc tgaacgcccg agccgatgcc gtctccgaga gcccgcggct ggagcgcatc
    13741 gacgtcgact gagaccggag gatcgcctat ggcaaccctc gaccaaggac tgctgcggaa
    13801 gctcagcgac tggtccccgg cccatccggt gacctccctc tatctcgacg tggacggccg
    13861 ccgccatccg agcaggaacg gctacctcgt ccggatgcgc gacctcttca aggggatgtc
    13921 gctggacgga ctcgatcgcg agcagcggag gtccgtggcc gaggacatgg aggcgatccg
    13981 ccgccacgtc gaggacgagc tcgaccgcag ccgcgtgggg ggtgttgcgg tcttctcgtc
    14041 ctcgaggagc ggcctctggg agagcgtgcc cgtgtcgcgc cccttccggg accggctcca
    14101 cgtcgggagc cggccgctgc tgatcccgct cgagggcgtg ctcgagcgct tcgagacctt
    14161 ctgcgtgctg gtggccgacc gggagcacgc caagctgctc tacgtccgtc aggacctggt
    14221 cgaggagctg aaggagatcc acgacgacgt ccccggccgg cacgagcagg gtggctggtc
    14281 gcaggcccgc ttccagcgtc acatcgagga ccacgtcctg cgccatctca agcacaccgc
    14341 gcaggtggcg ctgcgggcgg cacagcggag gcccttcgat cacctgatcc tggccgggtc
    14401 ggaggagctc gtcgccgagc tggagcgcga gctccacgac tacgtggcgc ggaaggtgct
    14461 cgaccggatc gtcctgccga tcacggtctc ggcccacgag gtccgggagc gggtgtccgc
    14521 gatcgaggcc gagctcgagt cccggcgcga gtccgaggcg ctcgagcgcc tcacgatcga
    14581 ggcgagagcc gggacggggc gggccgtggc ggggctggcg gggacgctcg aggccctgga
    14641 gtcgggccgc gtggagacgc tcgtggtggc cgccgacctc cgggcggacg gccggcggtg
    14701 cgctcgctgc gggcacctgt ccctggcgga ggtccggtgc gccgcgtgcg gcgcggacac
    14761 ggaggccgcc gaccacatcg tcgaggaggc cgtcgagatc gccgtgcggc agaagtgccg
    14821 cgtggagacg atcgggggcg cggaggagct cgcggccctc ggaggcgtcg gagcgctgct
    14881 ccgcttctga gggatgggcg agcggcctct ggcgatcggc ctcgacgtcg gaggcagcaa
    14941 ggtctcgggc gtcctgatgt cggacgaggg atcggtgttg gcctcggagc tgctggcgtc
    15001 acccgcagac gacgtcgagg cgatggtgga gacggtggtc ctcgcggcgg agcggctccg
    15061 ggccaagggt cccgccgcgg ccgtggggct gggcgcggcg gggatggtcg agcacaccac
    15121 cggcgtcctc cgctccgccc cgaacatcgc gtggacggag gtgccgctcc gcgacctcgt
    15181 ccggcggagg atcgggctcc cgtgcgtggt ggacaacgac gcgaacgccg cggcgtgggg
    15241 ggagttccgg ttcggggccg gtcgcggcct gcgacacgtc ctcctggtca cggtggggac
    15301 gggcatcggg ggcgggctga tccagaacgg gaggctctac cgcggcgccc acgggttcgc
    15361 ggcggagatc gggcacatca tcgtggagcc ggacgggccg ccgtgcggct gcgggaacag
    15421 ggggtgctgg gagcagatgg cgagcgggca ggcgctggag cgcatggcgc gggaggcggc
    15481 tctcggcgac ccggcggggc ccatcgcgcg gacggccggc ggacggcccc ctgtcggccc
    15541 cgacgtggtg gcggccgccg cggccggcga tccagccgcg ggaacgatcc tcgagacggt
    15601 tggccgccgg ctgggcgagg ggctggccgg gctggccaac gtcctcgacc ccgaggccat
    15661 cgtggtggga ggggggatgg ccgagatcgg ggccccgttc ctcgacccgg cccgggtccg
    15721 cttcctggag gcggtcgagg cgcccgatca ccgacccgag atcccgatcc tgcaggccgt
    15781 cctggggaat gacgccgggg cgatcggcgc ggcggcgatg gcgctcgagc ccttggagga
    15841 cggatgaaga ccggcgtcgt cctccccctg ttctccggaa acccggagaa ggtgctggcc
    15901 gcggcgcgcg agactgaggc gctcgggtac gacggcgcgt tcgtcttcga tcacttcttc
    15961 ccgcccggcg cctcctcgga tcggccctcc ctcgaggcgt tcacgacgct cgcggcggtg
    16021 gcggcggtca ccgagcggat ccgggtgggg acgctcgtca cgcgcgcgtc gctccgtccg
    16081 cccgggatcc tggcgaagca ggcctcgtgg atcgacgcgg cctcggacgg acgcctgatc
    16141 ctggggattg ggaccgggga cgagaccgac ggcgacgagc accgggcctt cgggctcccg
    16201 ctgatgggcc gagacgagcg gcgggcgcac ctcgaggaga cggcgacgac gctccgggcg
    16261 ctgttcgagg ggcgggacgg ggcgctcgag gggccgctgg cgccgtcggc cagcccgccg
    16321 atctggatcg gcgggctggc cgacgcgatc gtccgcatcg cggctcgcca tgcggacggc
    16381 tggaacggct ggggcctgga cgtcccgacg ttcgcccgca aggccggcct cctccgagag
    16441 ggggcggggg atcgcggggt cgaggcgacc tgggcgggga tcgtcctcgt gggccgggac
    16501 gcggccgagc tcgcggagct caaggcccgc cgcgccgacc ggggcatgga ggacgcctcc
    16561 tggagcggga ccgccgccga gctcgtgggc ttcctgaccg agctggggcg ggcgggggcg
    16621 gggtgggcca tcatggtggc cgccgggccg cgcgatcgcc gtgagctgat cgcggaagag
    16681 gtcctccccg agctttgaca ccggtccgag gcggtccgta gtctccgtcg ggcccgaggc
    16741 ggaaggagca tcgatggcga agcgagtcgt gatcatgggg gccgcgggtc gggacttcca
    16801 caacttcaac gtggtctacc gcggggaccg ggaggtcgag gtcgtcgcct tcaccgcgac
    16861 gcagatcccg ggcatcgaga accgccgcta cccgccggag ctcgccggcg accgctaccc
    16921 ggagggcatc ccgatccggc tggaggacga gctcgccgat ctcatcgcgc gggagcgcgt
    16981 ggacgaggtc gtcttcgcct actccgacgt ctcgcacgaa tacgtgatgc accgagccag
    17041 cgccgtcctg gccgccggcg ccaccttcac cctgctcggc ccggaggcca cgatgctgga
    17101 ctcgacgaag ccggtcgtgg cggtctgcgc ggtccggacc ggcgccggca agagccagac
    17161 cacgcgcgcc gtcgtccggg ccctgaaggc ccagggccgc aagccgggcg tcgtgcgcca
    17221 ccccatgccg tacggggaca tcgtcgccca gcgcgtccag cgcttcgcca cgatggacga
    17281 cctcgacgcc gcggacgtca ccgtggagga gcgagaggag tacgagcccc acatcgcctc
    17341 cgacactgtc gtctacgcgg gcgtggacta cgcggagatc ctggaggcgg tccagcagga
    17401 ggccgacgtc gtcatctggg acggagggaa caacgacctc cccttctacc ggcccgacgt
    17461 ccacctcgtg gtggccgacc cgcaccggcc cgggcacgaa actcgctatc accccggcga
    17521 ggcgaacctc cgcatgggcg acgtgatcgt gatcaacaag gtggacacgg cggagcccga
    17581 gaacgtcgac gccgtcgagg ccaccgcccg cgagctcaac ccggacgccg tgatcatccg
    17641 cgcgaacagc cccgtgaccg tggacgaccc tggcgccgtg gagggagccc gggttctcgt
    17701 gatcgaggac ggacccaccc tctcgcacgg ggagatgacc tacggcgccg gcgtcgtggc
    17761 cgcccggcag cacggagccg cggagatcgt ggacccacgg gagtgggctg tcggcagcct
    17821 gaagacggtc tacgagaagt acccggtggg ccccgtgctg ccggcgatgg ggtactcgga
    17881 cgagcagatc cgcgagctcg aggagacgat cgacgcggtc ccctgcgacc tcgtgatggt
    17941 ggccactccc gtggacctct cccggatcgc gtcgttctcg aagccctccg tccgcgtggg
    18001 ctacgagctg gaggagggtg aggggcagcc cacgatcgcc gaggtcctcg ccgcgcggct
    18061 ccccgcggcc gggggctgag gtgcctcgcg tcgtcatcgc cctgggcggc aacgcgctcc
    18121 tccagcgcga ggaggaggac acggcccagg tgatggtccg caacgcgcgc accgcggccg
    18181 agcaggtggc cgacgtggcg gcctcgggct gggagatcct cgtcacccac gggaacggcc
    18241 cgcaggtcgg ccgcatcctc ctgcagaacg aggccgcccg ggactgggcc catccgatgc
    18301 ccctcgacgt gtgcggcgcc atgagccagg ggcagatcgg ctacctcctc caggtgacga
    18361 tcggggacgt cttcttcgag cgggggatgg agcggccggt gaccacgctc ctcacgctga
    18421 cccgggtccg cgcggacgac cccgcgttcc agggcccgac gaagccgatc ggccccttct
    18481 acgaggaggc cgaggcggcg cgcctcgccg aggagcgggg ctgggtcgtg aaggaggtgg
    18541 gggagggcgg ctggcgccgg gtggtccctt cccccaggcc gtactcgatc gtggaggccc
    18601 cgatcgcgag gcagctcgcg gagtcggaga cgatcgtgat ctgtacgggc ggcggcggcg
    18661 tccccgtgat cgaggaaggt ccccggctgg tgggggtgga ggccgtcgtg gacaaggacc
    18721 acgcgtcggc gatcatggcc cgcgaggtcg aggcccaggt cctcatgatc ctcaccgacg
    18781 tggagggact gtacgagcgg ttcggcgagc ccgatcagcg gctgctggag cggctcaccg
    18841 tggacgacgc ggagcgggtg ctggactccc tccccgccgg ctcgatgggt cccaagctcg
    18901 aggcgtgcgt ggacttcgtc cggggcgggg gagagcgggc cgtgatcggg ccgctggacg
    18961 atgccctggc cgcgctcgag ggccgcaccg gcacctcgat cgtctccacc tgacgcccgg
    19021 gctaggcttc cacggatgga ccttcgcgtc gtcgtgtgga acgcccacgg gctccgggcg
    19081 ggcgtggccg ccgccgccga ggcgctcgcg cgcctccggc ccgatctggt catgctcaac
    19141 gaggtggggt ggagcggatg gaggctggcg cggctggccc gggcgctcga cgcgcgcgcc
    19201 gcgtcggggc tccggcgcct ccggtggagt gtgacgaacg ccgtactcgt gctgccgcct
    19261 tggcgcctgc tcaggacgcg gacgatccgc ctcccggggc ggtccaggcc gcatcgcggg
    19321 gtcgtcgcgt ccctgatcgg ccggtccggg taccggctga cggcggccag cgtccacctc
    19381 gggctctccg atccggagcg ccgcgagcac gcccgggatc tcaccgacag gatcctcccc
    19441 gagctgcgcc cccccgtact cctgggcggc gatctgaacg agccgccgga aggcccggcg
    19501 gccaggtgga tggccgagcg cctctgggac gcgttcgccc aggccggcca agggccgggg
    19561 gagaccttcc ccgcccgcga cccccgggcc cgcatcgact acctgctcgt ccccgagggg
    19621 gtgacggtgg cgcgagcctt cgtcgaccgc gacgcggcgg aggcctccga ccacctgccg
    19681 ctcgtcgtcg atctggagct tgcctagccg cgtgtcacgc gctcgatgaa cgcggcgctc
    19741 cgctggaaga tggtgggagc gtcgttgtcc agcgcggcga catggtagga gtcctggagg
    19801 aggacgagct ccttgtcctc gctgccgatc cgatccagga gccattccgc cgtgcccctc
    19861 gggacgacgt ggtcctcggg ggagtggaag agcaggaggg gctggacgat cgaggggatc
    19921 gccgcttcga gccgcttcat catcccggcg acctccgaga cggctcggag gggcacgcga
    19981 ggatagggca gctcgtctcc acccggcttc ttgatgtcgt tcccgatccc cttgatggtg
    20041 cggaggaacg ggcggacgac cggcagccac acgtgccgag ggtcccgtac gtaggggttc
    20101 acgagcacga tcccccggac gtccccggta cgacgggacg ccagatcgag agcgatggtg
    20161 ccgccgaagg actgggcgca gatgacgacc cgacctccgt cacctcgaag cttcgtcagc
    20221 gccgcatccg ccgcggcgac ccattcgtcc ccacgccggg tcgccaggtc ctgccatgtc
    20281 gtcccgtgtc ccggaaggcg cgggccgcag accgtgaagc ccctctccgc gagggcctca
    20341 ccccacggcc tgaggctcga cgggttgccg gtgaacccgt ggagcatgag cacccgcgcg
    20401 tcccctcccg ggaaggagaa cggctcggcc ccggggacga cctcggcgct catcccacgg
    20461 cggcccgcac gcggtcgatc agcgcctcgg gatcgaaggg cttcacgatg aagttctcgc
    20521 gccccaccag gcccaggacg tcgtgcccca cctccggctt ccccgagacc acgacgaccg
    20581 gggtcccccg ggtcgcaggc gcggcccgga gctgggtgag caggctctcg ccccccacgc
    20641 cgggcatcat caggtccagg acgaccgcgt cgggtcggtg ccgctccagg agctcgagcc
    20701 cctcggctcc gccgcgcgcc tccagcacgt cgaagtcgtg gagctccagg ctgacgcgaa
    20761 cgagctgacg gacggagtcg tcgtcctcga ccaggaggat cgtcttcctg ggcgccaccg
    20821 ggtcaccgac cgcgcgggtt gggccggcgg cggagaccgg tgttccggaa gaggagggcc
    20881 aggaggtaca gcccgaagag ccccgccgcg agcgacaggg atcccaccac gatcacgaac
    20941 gtccattcga ggacgcccca tccactttcc acgcccgacc tcctcatcat cgcttgtcgg
    21001 cccagggggg agcctatagt ctagttgacc gcccggacga tcgaggatcg agatgttcta
    21061 ctggttcctg aagtacgtgc tgctcggccc gctcctccgg ctgatctaca ggccgaaggc
    21121 cgtggggctc gagcacgtcc ccgccgaggg gccggtcatc ctggcctcga accacctctc
    21181 gttcgtggac agcctcttca tccccctgct ggtgaagcgc cccgtggtgt tcctggggaa
    21241 agcggactac ttcgactcgt ggaggacgcg ctggttcatg aaggccgtcc gcgtgatccc
    21301 cgtgcggcgc accgggggct ccgcgagcga ggctgcgatc cggacgggcg tggaggcgct
    21361 gggggagggc ctcgtgatcg ggatctaccc ggaggggacc cggagccccg acggccggct
    21421 gtaccggggg aagaccggcg tggcccggat ggccctggag gcgcagaccc ccgtcgtgcc
    21481 gatcgcggtc gacgggacgg tcgacatcat gcctctcgac aagaagatgc cccggctgcg
    21541 ggggcgagtg acggtgcgct tcggcccgcc cctcgacttc gcgcgctact acgaccgccc
    21601 gcgcgaccgc ttcgttctgc gttcggtcac cgacgagatc atgtacgaga tcatgatgct
    21661 ctcggggcag gagtacgtgg acgagtacgc cgcgaaggtc aagaaggagc tcgcggctca
    21721 ggagcggacg gtccgcgtgc ccgacaccga gacggtcgac caggactcgc gcaccggcgc
    21781 ggcctgatca gccgcgggcc caggccaggg agcgctcgac ggcccttcgc cagtcgtcgt
    21841 agcgctcggt gaggtcctcg ccgctcggga ggaactccgc gtcgagctcc caggccctcg
    21901 ccgcctcgtc cttcgaggac cagacgccgg tcgagagccc cgccaggtag gcggcgccca
    21961 gcgaggtcgt ctcctggacc ttagggcgcc gcaccggcac cccgagcagg tcggcctgga
    22021 agcggcacag caggtccatc acgctcgcgc cgccgtcgac cttgagctgg ctcagcccga
    22081 cctccgagtc ggccaccatc cggtcgacga cgtccctcgt ctggaacgcc atcgactcga
    22141 cggcggcgcg agcgaggtgc gctcggttcg tgccccgggt gatccccacg atcgtccccc
    22201 gcgcgtgcgg gtcccaccaa ggggcgccca gcccggtgag ggccgggacg aagtagacgc
    22261 cctcggtgtc cgggacggag gcggcgagag ggccgatctc ggcggcggcc cggatgatcc
    22321 ccagcccgtc gcgcagccac tggatcgcgg cgccggtgac gaagatcgag ccctccagcg
    22381 cgtagcgaac gccctcctcg tcggagtagg cgatcgtcgt caggagcccc ttggcccgcg
    22441 gcgcgtggtc ccccgtgttg aggagcacga aggagccggt gccgtaggtg ttcttcgcct
    22501 ggccgggcgt gaagcaggcc tggcccacga gcgcggcctg ctgatcgccg gccacgcccg
    22561 cgatcgggac gcggatgccg aggaacgcgt cggggtcggt ctcgccgaag gagcctgacg
    22621 aaggcccgac ctccgggagg accgcctcgg ggatccgcag gcgctccagc agctccgggt
    22681 cccagtccag gcggaggatg tcgaagagca tcgtgcggga ggcgttcgtg tggtcggtga
    22741 cgtgggcccg gccgccggtg agccgggaga tcaggtaggc gtccaccgtc ccgaaggcga
    22801 gccggcccgc ctcggcggcc tcccgagccc cgtcgacctg gtcgagcagc caggccagct
    22861 tggtgcccga gaagtaggga tcgatcacca gcccggtgcg ctcgatcacc cgctcctccc
    22921 agccctcgtc caggagctcc gcgcacatgg gggcggtccg ccggtcctgc cacacgatcg
    22981 cggggtggac cgggtggagc gtctcccggt cccagagcac cgtcgtctcg cgctggttcg
    23041 tgatgccgag ggcggtgagg tccgaggcgc ggagatccgc ggcgcgcagg gcggcgagcg
    23101 tggcctcctg ggtggccgcc caccagtcgt cgggctcgtg ctccacccac ccgggccggg
    23161 ggaacgactg ggcgaactcc cgatagccgg tgcccaggac ggagcccgcc tcgttcacga
    23221 tcagggcctt cacgccgctg gtgccggcgt cgatcgccag gatccgcgcc acgggcgaca
    23281 tgctagaggg ggcccggcgc tgggactacc cgcggggcac gaggatctcg gcctcgacgt
    23341 ggctcaggct gaccccgagg tactcggcgg acgcctggga gaggtcgagc acgcgcccct
    23401 cgatgaaggg tccgcggtcg ttcacgagga cggtggcgca gcgccccccg tgccgaacgt
    23461 ggaggaacga ggggaggggc agggtgcggt gggccgcggt gaagagccgg gggtcgaaga
    23521 tggcgccgct ggcggtgggc cgtccggcga agtcccagcc gtaccaggag gcgatcccct
    23581 cgaagcccga cccggtgccg cggaggccgg acgggatgtc gcagccccgg ccgccgtcgg
    23641 ggccgagcag tgcgaggagc tcggactggt cgagcccgcc cgtgatcagg ggcgcgcggg
    23701 cgcgcgcgcg ccgctcggcc g
//
LOCUS       DASUTZ010000019         6840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1300564, whole genome shotgun sequence.
ACCESSION   DASUTZ010000019 DASUTZ010000000
VERSION     DASUTZ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300564"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1459)
                     /locus_tag="VF097_00930"
     CDS             complement(<1..1459)
                     /locus_tag="VF097_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6261388.1"
                     /translation="MEYRGRIFGKSVLEAQFRAFYGTDLFLGELLAAEDAERGRRFLF
                     RVIDVTYGSDSSDPRWAERAAGAYMAGDAAGTPYELRDPEQRLYKVARCAPLGYIDDA
                     GEFRKPKSLPTQFSKVTSPTVEDFEFLRGRMGDLRVGLLRSGEDTIDLEVGIRGETLT
                     SHVGVFATTGMGKSNLMKVLSGTILESQGRYAVLLFDPHGEYLDGGGGVRRGLRDHPW
                     AGDRLKVYATREGGGHTALRLALSELTLGDLQTAYEWSHAQEDAIWRLQRLYGEDWIA
                     AVADEALDPQELAEQVGAAASTMQVIQRRARRILELPCISRDPSQPSLSERIVRELQE
                     GYSVLVETSGLSSMEEILVASVITRRLLDTYANAYLDDREGFDRLPPTLVALEEAQRV
                     LSRAKRSDENVFPRVAREGRKFKVGLCAVSQQPKLIDDELLSQFNTFFVLGLADEKDR
                     NILRASSKQDVSDLGPEIQTLMPGEAIVTNLDAPFA"
     gene            complement(1461..2525)
                     /locus_tag="VF097_00935"
     CDS             complement(1461..2525)
                     /locus_tag="VF097_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HEX6261389.1"
                     /translation="MATEGGPRLAGVDLTEATGLEDAAAFVVDLLRRPAGEIRVLEGQ
                     LGLDEDLVVEGHPDAFELSPIEPSDPGTVWAVDGGSCTLADGRSFQVVGTRAARVRFR
                     EGRTDLVETPPLSVRAVSPAEMRRIGRDALTELCGREPERLPEMPRPVDALREWGEWA
                     EVRRTVSEASGGDLVLVDGSLHGGPLVPPEVVRAVHEDAVQRGVSLAGVVKASTLFWG
                     RNAPLVTLLKRRGDRELGPVPWAARISTDPVFGRLYVGEIFVAHLAGTAPHAFRVDVA
                     RGPLEPLEVLARLAGVADDPAFVGYPYPLARAHQAARVAGYSVADLRRELRGALAVAG
                     MSEDDVEVLFQDFHEVLNRS"
     gene            2777..3079
                     /locus_tag="VF097_00940"
     CDS             2777..3079
                     /locus_tag="VF097_00940"
                     /inference="COORDINATES: protein motif:HMM:NF025204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 family protein"
                     /protein_id="HEX6261390.1"
                     /translation="MDDQVETATEEENQAPAEAGAEDEESLDELIAKKTSEDAAEDES
                     EEESVLKLEPEERVGGSLPSNVQPQQSSEFTCRNCFLVKSKSQLADRKRMLCRDCA"
     gene            3001..5322
                     /gene="lnt"
                     /locus_tag="VF097_00945"
     CDS             3001..5322
                     /gene="lnt"
                     /locus_tag="VF097_00945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX6261391.1"
                     /translation="MSELLPRQEQEPARRPEADALQGLRLASAPVRLPLALPAAALSG
                     LLLWMGFAPVDLDAIGFVALVPILWAVRGAGGRRGALLGLVFGLTYFGILMSWLLPLT
                     ILGWSVLALGSAGWMALLFAAGAAVWRDDRPIVSALAFGAVWVSVEWMRGVLPFGGFT
                     WGGLGVTQHDNPLLLPLASVAGSWGISFVLAAVNALVLEALERVRAGWGRPVRLLGIA
                     VALALLPALIPTAAPDGPPVEIAVIQGNVPRDVAARDRLIVDRIVAENHARLHRTLAE
                     DPPDLAVWPENALDQDPRRDPSLAALVEGAISSVGSPTLVGAITFEDDGALFNENLLY
                     TGRGEIVGRYVKNHLVPFGEYVPFRARLEGFVPDIERVREDLTPGTEPGRFPVPGAPR
                     ASFASVICFENAFPDLVRQWVTADMGLLVISTNNSTFGTGPAARQHLALSEMRAVETG
                     RWVVHGALTGISGIVDPDGRVRQKTPLFDSAVLRASIPQGEGRTLFNVIGGWLPAAFL
                     LAASLALVAPRRRRRRVVPPLTASPRICVVVPTYDEAATIEEVVLGVRAVGERVEVLV
                     VDDASPDGTAEIVRGLMGEGRVRLLEREEKRGLATAYLEGFRAALDEGYDLIVEMDAD
                     LSHDPGDLRALLAGARSHHVTIGSRYVPGGSIPDWGLGRRILSRGGNLYARLLLGLPV
                     ADATSGFRVYRADALRELIDPPPTADGYAFQIELAYRAWRLGMSVGEVPITFRDRTHG
                     RSKLSQRIVAEALWKVMAWGVRDRLVRRRPPAA"
     gene            5354..6469
                     /locus_tag="VF097_00950"
     CDS             5354..6469
                     /locus_tag="VF097_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HEX6261392.1"
                     /translation="MDDTLLTIGIEEEFQIIDERGELRANIDAVMARAEQKPELEPED
                     VKPEMLQSVVEVGTKVCSNIKDARREVVRLRGQVVEILEEEGLRLASAGTHPFSHWKD
                     QPVTQKERYLSLEEEMQDVVRELLIFGLHVHIGIPDKDLRIDIFNEARYFLPHLLCLS
                     TSSPFWLGRNTGLKSYRSILWSRFPRTGVPPDFPTWDDFDHYVNLLVKTQSIPDGSKV
                     WWDLRPHHVYPTLEFRVCDAATKIEETICIAAIIQAFVAKLLKLRKQNLGFRKYPGYL
                     IRENKWRAVRHGLEGKLIDFGKQEEVPERDLVLEILDFVDDVVDDLGSRSEVNYARQI
                     LAEGTSAERQLRVYEETDDLRSVVDHICDETRQGLPA"
     gene            6529..>6840
                     /locus_tag="VF097_00955"
     CDS             6529..>6840
                     /locus_tag="VF097_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004000451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX6261393.1"
                     /translation="MTSTSAAAQRLRDLARLRRVRDRIDRDYAQPLDVEALARDAHMS
                     AGHLSRRFRSAFGESPYSYLMTRRIERAMALLRRGDLSVTDVCFEVGFSSLGTFSTRF
                     TE"
BASE COUNT          969 a         2426 c         2446 g          999 t
ORIGIN      
        1 gcgcgaacgg ggcgtcgagg ttcgtgacga tggcctcgcc cggcatcagg gtctggatct
       61 cggggccgag gtcggagacg tcctgcttcg aggaggcccg caggatgttg cggtccttct
      121 cgtcggccag ccccaggacg aagaacgtgt tgaactggct cagcagctcg tcgtcgatca
      181 gcttgggctg ctggctgacg gcgcagagcc ccaccttgaa cttgcgtccc tcgcgcgcca
      241 cgcgggggaa gacgttctcg tccgagcgct tggcccggga cagcacccgc tgggcctcct
      301 ccagcgccac cagggtgggg gggaggcggt cgaagccctc ccggtcgtcg agataggcgt
      361 tcgcgtaggt gtcgagcagg cgccgcgtga tgaccgaggc cacgaggatc tcctccatcg
      421 aggacagccc cgaggtctct accagcaccg agtacccctc ctggagctcg cggacgatgc
      481 gctccgagag cgagggctgg gacgggtcgc ggctgatgca cggcagctcg aggatgcgcc
      541 gggcccggcg ctggatcacc tgcatcgtgc tggcggccgc gcccacctgc tcggccagct
      601 cctgggggtc gagggcctcg tcggccaccg ccgcgatcca gtcctcgccg tagaggcgct
      661 ggaggcgcca gatcgcgtcc tcctgggcgt ggctccactc gtaggccgtc tggaggtcgc
      721 ccagggtgag ctcggacagg gccagtctga gcgccgtgtg gccaccgccc tcgcgggtgg
      781 cgtagacctt gaggcggtcg ccggcccagg ggtggtcgcg caggccccga cggaccccgc
      841 cgcccccgtc caggtactcg ccgtgcgggt cgaagagcag gaccgcgtac cggccctggg
      901 actcgaggat cgtccccgac aggaccttca tcaggttgga cttgcccatg ccggtcgtgg
      961 cgaagacgcc cacgtgcgag gtgagggtct cgccccggat ccccacctcg aggtcgatcg
     1021 tgtcctcgcc cgaccgcagc aggcccacgc gcaggtcgcc catccggccg cggaggaact
     1081 cgaagtcctc cacggtgggg gaggtgacct tggagaactg cgtggggagg gacttcggct
     1141 tgcggaactc gcccgcgtcg tcgatgtagc cgagcggcgc gcaccgcgcg accttataga
     1201 gtcgctgctc cgggtcgcgc agctcgtagg gggtgccggc ggcgtccccg gccatgtagg
     1261 cgccggcggc gcgctcggcc caccgggggt ccgaggagtc cgacccgtag gtcacgtcga
     1321 tcacgcggaa gaggaagcgg cgcccgcgct cggcgtcctc ggcggccagc agctcgccca
     1381 ggaagaggtc ggtgccgtag aaggcgcgga actgcgcctc caggacgctc ttgccgaaga
     1441 tcctgccgcg gtactccatg tcagctcctg ttcagcacct cgtggaagtc ctggaagagg
     1501 acctcgacgt cgtcctcgct catgccggcc acggctagcg ccccccggag ctcccggcgg
     1561 aggtcggcca ccgagtagcc ggccacgcgc gcggcctggt gggcccgggc caggggatag
     1621 gggtagccca cgaaggcggg gtcgtcggcc acgccggcca ggcgggccag cacctcgagc
     1681 ggttccagcg gcccccgggc cacgtccacg cggaaggcgt gcggcgcggt tccggccagg
     1741 tgggccacga agatctcgcc cacgtagagg cggccgaaga ccgggtcggt cgagatccgg
     1801 gcggcccagg gaacggggcc gagctcgcgg tcgccccggc gcttcagcag ggtcaccagc
     1861 ggcgcgttgc ggccccagaa cagcgtggag gccttcacca cgccggccag cgagaccccg
     1921 cgctgcacgg cgtcctcgtg gacggctcgc acgacctcgg gcggcaccag cggtcctccg
     1981 tggagcgagc cgtccacgag gacgaggtcc ccgcccgaag cctccgagac cgtcctccgc
     2041 acctccgccc actcgcccca ctcgcgcagg gcgtccacgg ggcgtggcat ctccgggagc
     2101 cgctccggct cgcgtccgca cagctcggtg agcgcgtcgc gcccgatccg gcgcatctcg
     2161 gcgggggaga cggcccgcac gctcagcggc ggcgtctcca cgaggtccgt ccgcccctcc
     2221 cggaagcgca cgcgagccgc cctcgtcccc accacctgga acgagcggcc gtcggccagc
     2281 gtgcaggagc cgccgtccac ggcccagacc gtcccgggat cggacggctc gatgggggag
     2341 agctcgaacg cgtccggatg cccctccacg acgaggtcct cgtccagccc gagctgtccc
     2401 tcgagcaccc ggatctcgcc cgccggacgc cggagcagat cgaccacgaa ggcggcggcg
     2461 tcctcgagcc ccgtggcctc ggtcagatcc acgccggcga gcctcggtcc tccctccgtc
     2521 gccatcggct gaaggatatc ccgccccgcc gacgcgatcg cgtagcccgt ccggttgaca
     2581 cccctcaggg tgccgactag attgagccgc gcagcgccgg gccaccggcg cggcgacggg
     2641 ccaagaggct gagtagagaa cgcggcgccc cggcgccgcg gccccaaagg tctcttggcc
     2701 cttcgctttc cgtcctcgat ccggcttcca tccgacgacc ggcgggtaga atggcgcagc
     2761 cttgatggag gatgcgatgg acgaccaggt tgagaccgcc accgaagaag agaaccaggc
     2821 cccagccgag gccggggcag aggacgaaga gagcctcgac gagctgatcg ccaagaagac
     2881 ctcggaggac gccgccgagg acgagtccga ggaggagtcg gtgctgaagc tcgagccgga
     2941 ggagcgggtc ggcggtagcc tgccctccaa cgtccagccc cagcaatcgt cggagttcac
     3001 gtgtcggaac tgcttcctcg tcaagagcaa gagccagctc gccgaccgga agcggatgct
     3061 ctgcagggac tgcgcctagc ctccgccccc gtccgtctcc ctctcgctct gcccgccgcg
     3121 gccctctcgg gcttgctcct ctggatgggg ttcgcccccg tggacctcga cgcgatcggg
     3181 ttcgtggcgc tcgtgccaat cctgtgggcg gtgcggggcg cggggggccg gcggggggcg
     3241 ctgctggggc tggtcttcgg cctcacctac ttcgggatcc tcatgtcgtg gctgctcccg
     3301 ctgaccatcc tgggatggtc ggtgctggcc ctcggctccg cgggatggat ggccctgctg
     3361 ttcgccgcgg gcgcggccgt ctggcgcgac gaccggccga tcgtgtcggc cctggccttc
     3421 ggcgcggtct gggtgtcggt ggagtggatg cgaggcgtgc ttccgttcgg tggcttcacc
     3481 tgggggggcc tgggggtgac ccagcacgac aacccgctgc tcctgccgct ggcctcggta
     3541 gcgggctcct gggggatctc gttcgtcctg gccgcggtga acgccctggt gctggaggcc
     3601 ctggagcggg tgcgcgccgg ctgggggagg cccgtccgtc tgctggggat cgcggtggcc
     3661 ctcgcgctcc tcccggcgct catccctacg gccgctccgg acggccctcc ggtcgagatc
     3721 gccgtgatcc aggggaacgt gccgcgcgac gtcgcggccc gggaccgtct catcgtggac
     3781 cggatcgtgg ccgagaacca cgcccgccta caccggaccc tggccgagga ccctcccgac
     3841 ctggccgtgt ggcccgagaa cgccctggac caggacccgc ggcgcgatcc ctccctggcg
     3901 gcgctggtgg agggcgcgat ctcctcggtg ggcagcccca ccctcgtcgg cgcgatcacg
     3961 ttcgaggacg acggggccct cttcaacgag aacctccttt acacgggccg gggtgagatc
     4021 gtggggcggt acgtgaaaaa ccacctcgtg cccttcgggg agtacgtgcc gttccgggca
     4081 cggttggagg ggttcgtacc ggacatcgag cgggtccgcg aggacctcac gccggggacc
     4141 gagcccggcc ggttccccgt cccgggcgcg ccgagagcct cgttcgcctc ggtgatctgc
     4201 ttcgagaacg ccttccccga cctcgtgcgg cagtgggtga cggccgacat ggggcttctc
     4261 gtgatctcca cgaacaactc cacgttcggg accgggccgg ccgctcgcca gcacctggct
     4321 ctcagcgaga tgcgggccgt cgagaccggg cgctgggtgg tccacggcgc cctcaccggc
     4381 atcagtggga tcgtggaccc ggacggccgt gtgcgtcaga agacgccgct cttcgattcg
     4441 gcggtcctgc gagcctcgat cccgcagggg gaggggcgga ccctgttcaa cgtgatcggt
     4501 ggctggctgc ccgcggcctt cctcctcgcc gcgagcctgg ctctcgtggc cccacgccgc
     4561 cgtcggcgga gggtggtccc cccgctcacc gcctcgcccc ggatctgcgt ggtcgtcccc
     4621 acctacgacg aggcggccac gatcgaggaa gtcgtcctgg gcgtccgcgc ggtgggggag
     4681 cgggtcgagg tgctggtcgt ggacgacgcc tcgcccgacg ggacggccga gatcgttcgc
     4741 ggcctgatgg gggaggggcg ggtccggctc ctggagcggg aggagaagcg aggcctggcc
     4801 accgcctacc tcgaggggtt ccgcgcggcg ctggacgagg gctacgacct gatcgtggag
     4861 atggacgccg acctctccca cgacccagga gacctgcgcg cgctgctggc cggggcccgc
     4921 tcgcaccatg tgacgatcgg cagccgctac gtgccggggg gctcgatccc ggactggggg
     4981 ctggggcgcc gcatcctgtc tcgcggcggg aacctctacg ccaggctgct gctggggctc
     5041 ccggtggccg acgccacgag cgggttccgc gtgtacagag ccgacgcgct ccgcgagctg
     5101 atcgacccgc ctcccaccgc cgacggctac gccttccaga tcgagctcgc ctaccgcgcc
     5161 tggcgcctgg ggatgtcggt gggggaggtg ccgatcacgt tccgggaccg cacgcacggc
     5221 cgctccaagc tctcccagag gatcgtggcc gaggccctgt ggaaggttat ggcctggggc
     5281 gtgcgcgacc gcttggtccg ccgccgcccc ccggccgctt gacgccgcgc ccttcgtacg
     5341 gtcgagtatc cccgtggacg acaccctcct gaccatcggg atcgaggagg agttccagat
     5401 catcgatgag cgcggcgagc tgcgcgcgaa catcgacgcc gtgatggccc gcgccgagca
     5461 gaagccggag ctcgagcccg aggacgtcaa gcccgagatg ctccagtcgg tcgtcgaggt
     5521 ggggaccaag gtctgctcca acatcaagga tgcgcgccgg gaggtcgtgc gcctccgagg
     5581 tcaggtcgtc gagatcctgg aggaggaagg actgcgcctg gcctcggccg gcacccatcc
     5641 gttcagccac tggaaggacc agccggtcac ccagaaggag cggtacctca gcctcgagga
     5701 ggagatgcag gacgtcgtcc gggagctcct gatcttcggc ctccacgtgc acatcgggat
     5761 ccccgacaag gacctgcgga tcgacatctt caacgaggcg aggtacttcc tcccacacct
     5821 cctgtgcctc tcgacctcgt cccccttctg gctggggcgc aacacagggc tcaagtccta
     5881 tcgctcgatc ctctggtcgc ggttcccgcg gaccggcgtg ccgcccgact tccccacctg
     5941 ggacgacttc gaccactacg tgaacctcct ggtgaagacg cagagcatcc ccgacggctc
     6001 gaaggtctgg tgggacctga ggccccacca cgtctacccg acgctggagt tccgcgtgtg
     6061 cgacgcggcc accaagatcg aagagacgat ctgcatcgcc gcgatcatcc aggccttcgt
     6121 ggccaagctg ctgaagctcc ggaagcagaa cctgggcttc cggaagtacc cgggctacct
     6181 gatccgggag aacaaatggc gcgccgtccg ccacgggctc gaggggaagc tgatcgactt
     6241 cgggaagcag gaggaggttc ccgagcggga cctcgtcctg gagatcctgg acttcgtgga
     6301 cgacgtcgtg gacgacctcg ggagccggtc cgaggtcaac tacgccaggc agatcctggc
     6361 cgagggcacc agcgccgagc gccagctccg ggtctatgag gagacggacg acctgcggtc
     6421 ggtcgtggac catatctgcg acgagacgcg ccagggcctg ccggcgtaag cccaggaggc
     6481 gtcgcgggct cgggggagac cccggtccga cttgatacca tcgcgccggt gaccagcaca
     6541 tccgccgcgg cgcagcgcct gcgcgacctc gcacggctgc ggcgcgtccg cgaccggatc
     6601 gatcgggact acgcccagcc gctggacgtg gaggccctcg cccgcgacgc ccacatgtcg
     6661 gccgggcacc tcagccgccg gttccggagc gccttcggag agtcgcccta cagctacctg
     6721 atgacgcgtc ggatcgagcg cgccatggcg ctgctgcgcc gtggggatct gagcgtcacc
     6781 gacgtgtgct tcgaggtggg gttctcgtcg ctggggacct tcagcacccg tttcaccgag
//
LOCUS       DASUTZ010000020         5216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_781856, whole genome shotgun sequence.
ACCESSION   DASUTZ010000020 DASUTZ010000000
VERSION     DASUTZ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5216
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781856"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1252)
                     /gene="sat"
                     /locus_tag="VF097_00960"
     CDS             complement(95..1252)
                     /gene="sat"
                     /locus_tag="VF097_00960"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEX6261394.1"
                     /translation="MTDTIEPHGGELVDLVLPEAEAASAAEDAQHMPKIELGPREIAD
                     LEMLAVGAMSPLTGFMTEKDYTSVLHEMRLANGLVWTLPITLSVDEEGMKQVGRSDRV
                     ALTIGGEIHAILEVAETFRRDREVEAESAFGTTDDAHPGVAALRTAGDWVLGGRVSAL
                     KVPAHETFVDHRRTPAETRQAFAERGWKRVVGFQTRNPVHRAHEYIQKCALEITDGLL
                     LHPLMGETKADDIPADVRMRCYLVLLDGYYPKDRVLLSIFPAAMRYAGPKEAIFHAIV
                     RKNYGCTHFIVGRDHAGVGDFYGTYDAQRIFDEFEESDLGIIPLFFEHAFWCERCEGM
                     ASTKICPHGEEDRISLSGTRVREMLRTGERPPMEFSRPEVADVLIDAMRDQ"
     gene            1307..2041
                     /locus_tag="VF097_00965"
     CDS             1307..2041
                     /locus_tag="VF097_00965"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEX6261395.1"
                     /translation="MPPLDLPALNARFEDADPAHIVAWALDASERPAVASSFQVESTV
                     VIHLATRIRPDVPVLFLETGFHFRETLELKGRLTRLLGLTVVELRGETTPQEQADVHG
                     PRLYERDPALCCELNKVRPLDRALAGYDLWMTGLRRSSASTRARTRVVEPHRTGEGHE
                     LLKVNPVAGWTHARARAYLEEHGLPHHPLYDLGYASIGCAPCTRALFPGEDERAGRWA
                     GVEKVECGIHLPPPATATAPPPASPA"
     gene            complement(2010..3125)
                     /locus_tag="VF097_00970"
     CDS             complement(2010..3125)
                     /locus_tag="VF097_00970"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX6261396.1"
                     /translation="MVERPQPDHWSDPRPPAWTRRLILQALAWVALAWLAWTFLSRLS
                     QLLLWILIALFLSFAIEPAVNWLSKRGMRRGVATGLIFLAAALVGILSIAVMVPFVVQ
                     GVLAVVRNLPSWLDQASRLTEDWFGVTVSTEDLRREIFSAASTLQSFAGDIVGNVIGV
                     GGAALGAVFQVFTIGFFLFYFVAEGPRVRRGVCSLLPRDKQDDVLFVWGTAIDKTGGY
                     FYSRLLLALINGITFFVVLFLLGIPGAPALALFSAVVATFIPIVGTYVGAAVPLLVAL
                     ASVGVGRTLVVLGWVVLYQQIENLLLAPKISARTMELHPAAAFSAALAGGLLGGVTWA
                     FLALPIAATVQAAASAYVGRYEVIEDDLTRETPEEER"
     gene            complement(3118..3846)
                     /locus_tag="VF097_00975"
     CDS             complement(3118..3846)
                     /locus_tag="VF097_00975"
                     /inference="COORDINATES: protein motif:HMM:NF022655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HEX6261397.1"
                     /translation="MVRAVRRDAPRPRGPGRAILISVLVLLLVAVVVDLGLRFLAERW
                     LEERMQQTLALERRPDLDIGGFPFIPQFARGEVERVDLTLRDLRVQGLDLDEVTIGLR
                     RVAFNRADVLAGRHGSVRVRRGVATVEIDEPTLNGMLRDRGVPVSVELLGPRIRATWR
                     IEVEGREATATAEGTLDLEGTTLGFDPGEVSVDGGFGVPPAALGFRVDLPPLFPGLTY
                     QDVRVREAVLALRAALDDTVLDLG"
     gene            complement(3847..4242)
                     /locus_tag="VF097_00980"
     CDS             complement(3847..4242)
                     /locus_tag="VF097_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006078845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX6261398.1"
                     /translation="MTLEGKALELFEGRNFAHLATINPDGSPQVSPVWVHARDGMIEV
                     NTSRGRVKDRNMTKDPRVALSITDAKDPYVRASVQGEVVDQTEDGAVEHIDFLSEKYD
                     GVTPYPRLTPGMVRVIYRIRPGRVHVTGG"
     gene            complement(4276..4875)
                     /locus_tag="VF097_00985"
     CDS             complement(4276..4875)
                     /locus_tag="VF097_00985"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX6261399.1"
                     /translation="MRDVEGALRGGSMPEEAVERFVERAWDEVDGVVAYAGVDSPFGR
                     LIVAATRRGIVRLAYPGEADAVQELAERLTPRLVEAPERLDEARRELDDYFEGRRDRF
                     DLAVDLSLVRGKFTRKVLRATSRIPFGRTLTYTEVATKAGSPRGSRAAGNALGSNPIP
                     IVVPCHRVIHASGGLGGYTGGLDRKEFLLRLEGVLGETG"
     gene            complement(4872..>5216)
                     /locus_tag="VF097_00990"
     CDS             complement(4872..>5216)
                     /locus_tag="VF097_00990"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6261400.1"
                     /translation="SALRAYPDLADARNLRGWLLTIAKRKALDEHRARGRRPSPSERL
                     PEAAIDPPTEAEDGLWRAVRELPPKQREAVVYRFVCDLAHREIAELMSSTEAAARRNV
                     HEGLRALREVWR"
BASE COUNT          788 a         1935 c         1781 g          712 t
ORIGIN      
        1 ccgcccgcgc ctcccccgcc cgaggacgag gatcgggacg ggtagccgga ggtcgtcggg
       61 ttcctaagga agtctgcgac ggccaccccc gccttcactg gtcgcgcatc gcgtcgatca
      121 gcacgtcggc gacctccggc cggctgaact ccatcggggg gcgctcgccg gtccggagca
      181 tctctcgcac cctcgtgccg gagagcgaga tccggtcctc ctcgccgtgg gggcagatct
      241 tggtggaggc catcccctcg caccgctcgc accagaacgc gtgctcgaag aagagcggga
      301 tgatccccag gtcggactcc tcgaactcat cgaagatccg ctgcgcgtcg taggtcccgt
      361 agaagtcccc cacccccgcg tggtcccgcc ccacgatgaa gtgcgtgcag ccgtagttct
      421 tgcgcacgat cgcgtggaag atggcctcct tcggtcccgc gtagcgcatc gccgccggga
      481 agatcgagag gaggacgcgg tccttcgggt agtagccgtc gagcagaacg aggtagcagc
      541 gcatccgcac gtcggccggg atgtcgtcgg ccttcgtctc ccccatcagc gggtgcagca
      601 ggagcccgtc ggtgatctcg agcgcgcact tctgaatgta ctcgtgggcc cggtgcacgg
      661 ggttgcgtgt ctggaacccg accacgcgct tccagccgcg ctcggcgaag gcctgcctcg
      721 tctcggccgg cgtgcgccgg tggtctacga acgtctcgtg cgcggggacc ttgagcgccg
      781 acaccctgcc gccgagcacc cagtcaccgg cggtgcgcag agcggcgacg ccggggtgcg
      841 catcgtcggt cgtcccgaac gcggactcgg cctcgacctc gcggtcccgc cggaacgtct
      901 cggccacctc caggatcgcg tggatctcgc caccgatcgt cagcgccacg cggtcgctcc
      961 ggcccacctg cttcatcccc tcctcgtcca ccgagagggt gatgggcagg gtccagacca
     1021 ggccgttcgc cagacgcatc tcgtgcagga ccgaggtgta gtccttctcg gtcatgaagc
     1081 cagtgagcgg cgacatggcg cccacggcca gcatctcgag gtcggcgatc tcgcggggtc
     1141 ccagctcgat cttgggcatg tgctgggcgt cctcggcggc ggacgcggct tcggcctcgg
     1201 ggaggacgag gtccaccagc tcgccgccgt gcggctcgat cgtgtcggtc atagggcttc
     1261 ctcgggtcgg ggtcggcccg aggatagcag tcctatactt ggcctcgtgc cacccctcga
     1321 cctcccagcc ctcaacgcgc gcttcgagga cgcggacccc gcccacatcg tggcgtgggc
     1381 gctcgacgcc tcggagcggc cggccgtggc gtcgtcgttc caggtcgaga gcacggtcgt
     1441 gatccatctc gccacgcgca tcaggcccga cgtgcccgtg ctcttcctcg agaccgggtt
     1501 ccacttccgg gagacgctcg agctgaaggg ccggctcact cgcctgctcg ggctaaccgt
     1561 cgtggagctc cggggggaga ccacccccca ggagcaggca gacgtccatg gcccccggct
     1621 gtacgaacga gacccagccc tctgctgcga gctcaacaag gtgcggccgc tcgaccgggc
     1681 cctcgccggg tacgacctct ggatgacggg cctccgccgg agctccgcct ccacgcgggc
     1741 ccgaacccgc gtggtggagc cccaccggac cggggagggg cacgagctgc tcaaggtgaa
     1801 cccggtggcg ggatggaccc acgcccgggc ccgggcgtac ctggaggagc acgggctgcc
     1861 ccaccaccca ctctacgacc tggggtacgc ctcgatcggc tgcgcgccct gcacccgcgc
     1921 gctcttcccc ggcgaggacg agcgggcggg gcggtgggcc ggggtggaga aggtggagtg
     1981 cgggatccat ctcccgcctc ccgcgaccgc taccgctcct cctccggcgt ctcccgcgtg
     2041 aggtcgtcct cgatcacctc gtaccgcccg acgtaggcgc tggccgcggc ctgcacggtg
     2101 gcggcgatcg ggagggcgag gaaggcccag gtcacgccgc ccagcagccc cccggcgagc
     2161 gccgccgaga aagccgcggc cggatggagc tccatcgtgc gagcgctgat cttgggagcc
     2221 aggagcaggt tctcgatctg ctggtagagg acgacccatc ccaggacgac cagcgtccgg
     2281 cccacgccca cgctggccag ggcgacgagg aggggaacgg ccgcccccac gtaggtcccg
     2341 acgatcggga tgaaggtggc gacgaccgca ctgaagagcg ccagcgccgg agcgcccggg
     2401 atccccagga ggaacaggac cacgaagaac gtgatgccgt tgatcagcgc gaggaggagg
     2461 cgcgagtaga agtaaccgcc cgtcttgtcg atggccgtgc cccagacgaa gaggacgtcg
     2521 tcctgcttgt cccgcgggag cagggagcac acgccgcgcc ggacccgagg tccctccgcc
     2581 acgaagtaga acaggaagaa gccgatggtg aagacctgga acacggcccc gagcgccgcg
     2641 cctcctaccc cgatcacgtt gcccacgatg tcgccggcga aggactggag cgtcgaggcc
     2701 gccgagaaga tctcccttcg gaggtcctcc gtcgagacgg tgaccccgaa ccagtcctcg
     2761 gtgagccggc tggcctgatc gagccacgac gggaggttcc ggaccacggc cagcacgccc
     2821 tgcaccacga aggggaccat cacggcgatc gagaggatcc ccacgagcgc ggcggcgagg
     2881 aagatcagcc ccgtggccac gcctcgcctc atcccccgtt tggacagcca gttcacggcc
     2941 ggctcgatcg cgaacgagag gaagagggcg atcaggatcc acagaagcag ctggctcagg
     3001 cgggagagga acgtccaggc cagccaggca agcgccaccc aggccagcgc ctgcaggatg
     3061 agccgcctgg tccacgcggg gggtcgcgga tcgctccagt gatcgggctg cggtcgctca
     3121 accaaggtcg aggacagtat cgtccagggc ggcgcgcaga gcgaggaccg cctcgcgcac
     3181 ccggacgtcc tggtaggtca ggccggggaa gaggggcggg aggtccaccc ggaagcccag
     3241 cgccgcgggc ggcacaccga acccgccgtc cacgctgacc tcgccgggat cgaagcccag
     3301 cgtggtcccc tcgaggtcca gcgtcccctc ggcggtcgcc gtcgcctccc ggccctcgac
     3361 ctcgatccgc cacgtggcgc ggatgcgggg gcccaacagc tccacgctta cgggcacccc
     3421 ccggtcgcgg agcatcccgt tcagcgtggg ctcgtcgatc tccacggtgg ccaccccgcg
     3481 acggacgcgc accgacccgt gccgcccggc cagcacgtcc gcccggttga acgccaccct
     3541 ccgcagcccg atcgtcacct cgtcgaggtc cagaccctgg acccgcaggt cccggagggt
     3601 gagatcgacg cgctcgacct cgccccgggc gaactgcggg atgaagggga agcccccgat
     3661 gtccaggtcg gggcgcctct ccagggcaag ggtctgctgc atccgctcct cgagccaccg
     3721 ctcggccagg aagcgcaggc ccaggtcgac gaccacggcc accaggagca ggacgaggac
     3781 cgagatcagg atggctcgac ccggcccccg ggggcggggc gcgtccctgc ggacggcgcg
     3841 gaccactcag ccgccggtga cgtgaacgcg gcccgggcgg atccggtaga tcacccgcac
     3901 catgccgggc gtgagcctcg ggtagggcgt cacgccgtcg tacttctcgg agaggaagtc
     3961 gatgtgctcc accgcgccgt cctcggtctg atccacgacc tcgccctgca ccgaggcccg
     4021 cacgtagggg tccttcgcgt cggtgatcga cagcgcgacc ctcggatcct tcgtcatgtt
     4081 ccgatccttc acccgccccc gagaggtgtt cacctcgatc atcccgtcgc gggcgtggac
     4141 ccacaccggc gagacctgcg gcgacccgtc ggggttgatc gtcgccaggt gcgcgaagtt
     4201 gcgtccctcg aacagctcga gcgccttgcc ctccagggtc atgcgttcct cctccggggt
     4261 cggtcccctc gaaggttacc cggtctcgcc gagcacaccc tcgagcctta ggaggaactc
     4321 cttgcgatcg agcccgcccg tgtagccgcc gagcccgccg gaagcgtgga tcacgcggtg
     4381 gcaggggacg acgatcggga tcgggttgga tcccagcgcg ttgccggccg ccctcgagcc
     4441 gcgggggctg ccggccttcg tcgccacctc cgtgtaggtg agcgtcctgc cgaacgggat
     4501 ccgggacgtg gccctgagca ccttgcgggt gaacttgccg cggacgagcg agagatccac
     4561 cgccaggtcg aagcggtcac gcctgccctc gaagtagtcg tcgagctcgc gccgcgcctc
     4621 gtcgaggcgc tccggcgcct cgacgaggcg cggcgtcagc cgctcggcca gctcctggac
     4681 cgcgtccgcc tcgcccggat aggcgaggcg gacgatcccc cgcctcgtgg ccgccacgat
     4741 cagccggccg aacggggagt ccacaccggc gtaggccacg acgccgtcca cctcgtccca
     4801 ggcgcgctcg acgaagcgct cgaccgcctc ctcgggcatc gacccgcccc tcagcgcccc
     4861 ctccacgtcc ctcatctcca cacctcccga agggcccgca gcccctcgtg cacgttccgc
     4921 ctggccgcgg cctcggtcga gctcatcagc tccgcgatct cccgatgcgc caggtcgcac
     4981 acgaaccggt acacgaccgc ctcccgctgc ttcggaggga gctcccgcac cgcccgccag
     5041 agcccgtcct cggcctcggt gggcgggtcg atcgccgcct cgggcagccg ctccgagggc
     5101 gaggggcggc gcccccgggc ccggtgctcg tccagcgcct tccgcttcgc gatggtcagc
     5161 agccacccgc ggaggttcct ggcgtcggcc aggtcgggat acgcgcgcag ggccga
//
LOCUS       DASUTZ010000021         8502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1301029, whole genome shotgun sequence.
ACCESSION   DASUTZ010000021 DASUTZ010000000
VERSION     DASUTZ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8502
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301029"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="VF097_00995"
     CDS             <1..863
                     /locus_tag="VF097_00995"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX6261401.1"
                     /translation="ADGNVGARRQRLLRLPEVSPYHDGGDIAFGPDGMLYVVTGEAHS
                     PERAQDPEDVAGKVLRLEPDGSIPDNPLPGPVFSMGHRNSFGVCVDAGTGNVWQTENG
                     PSANDEVNLIEAGGNFGWPAVQGAADDPRFVDPEIDFVNVVVPTGCAVYPHDHLGPRS
                     EGALFFGDYGGTLWRAVLDQGGAGVERHHRFMTGLGGITDILVGPDERLYVVTNERIL
                     RLPAEIPSPEPSPSSPPSPGTSSEPEPTIDIPLVPSSEDGGIPWLLLAGVGILIGALA
                     AMWFARRGPT"
     gene            complement(870..1022)
                     /locus_tag="VF097_01000"
     CDS             complement(870..1022)
                     /locus_tag="VF097_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006547980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEX6261402.1"
                     /translation="MTTWEYATAPLIEHATQEILNQWGEDGWELVSVVSQPQGLIAYF
                     KRPTEG"
     gene            complement(1019..1453)
                     /locus_tag="VF097_01005"
     CDS             complement(1019..1453)
                     /locus_tag="VF097_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX6261403.1"
                     /translation="MDDVTPFHPGDEELKRILGEGKTIAVVGLSNRTDRPAYGVAAYL
                     KEQGYRIIPVHPKEEEQVLGEKVYPSLHEVPEPIDVVDVFVRADNTPPVAKDAVDVGA
                     KVLWLQEGIVSDEARQIAEGGGLEVVMGICIKKTHARLGVAG"
     gene            complement(1441..2016)
                     /locus_tag="VF097_01010"
     CDS             complement(1441..2016)
                     /locus_tag="VF097_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX6261404.1"
                     /translation="MADLAEMLRGALPPPRPDPPLPEGVPAAVVLPLLEAPEPALLFT
                     RRTDDLRHHRGEVSFPGGARHDEDPDLLTTALRETEEELGIPASAHDVLGRLVPMQAG
                     VSGYAVVPFVAHLGERPELRPNPMEVAEVLELPLELLAAVERLERVVWEGGSYDTYVY
                     EVNGQVVWGLTGRILHHLLRTLEREGWTWTM"
     gene            complement(2085..4040)
                     /locus_tag="VF097_01015"
     CDS             complement(2085..4040)
                     /locus_tag="VF097_01015"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEX6261405.1"
                     /translation="MKRSVVSTVVSVLLLSGALFAAPGSAGTAGSNQARGFFVPPEIG
                     QAVKRTPGNLGGPSDLFVEGELVVQFKKGVKRPAAMQIHAAHGGARVLNRVTGLGGRI
                     EHVKLPAGVSVAKAARRYQKDPGVVVAEPNFKYQWLSHITPPVDDPDIPHLWGLGNTG
                     QAHNISDPPPTTASGTSGADIAANAAWVTEDGDNTDPIVAVVDSGVRITHDDLDNNLW
                     VNGGETPANLVDDDGNGYVDDVHGCSFTPALCSNLSDDASGAGHGTHVAGTIAAEGEN
                     AQGIIGVCPHCRIMVTKFDLTTAGEVAAYAYARKNGARVINGSFGGAVWSKVSYNAIK
                     QLNQAGIVAVFAAGNDSLDNDTAAGNPVTGGISPAFPASYNLPNIIAVAASNHHDEYG
                     YFTGCNAPSPGDGFSKAECSFSNVGRESVDLAAPGVDIRSSVKTGDTAYDEFNGTSMA
                     SPHVAGAAALVRSEFPGLSPVQVKNKIMNGADKNLASLATFYSYILRGGRASGKFTRT
                     SGRLDAAGALAAGTGNATPKTDGDIPGAKRIAGSKKGRVAYPLDVNDLYKRRLQKGKR
                     YRVTLRVGKSDFDLWVWKPGTKQIWQFEPACFSGGRKCPVVRASFKGKGKNEVVTFKA
                     TKGGRYFFHVTAWLKFKGPYKLIIKQL"
     gene            complement(4196..5428)
                     /locus_tag="VF097_01020"
     CDS             complement(4196..5428)
                     /locus_tag="VF097_01020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6261406.1"
                     /translation="MLQVVDVETQSIEAYEPMIGREAVEELVELGRSLRGARVVQLNA
                     TAYGGGVSELLRSMLPIYRGLGIEAEWQVITGDDAFFEVTKGFHNALQGGDFELGEEG
                     RSEYLRHSEQNAAALEGDWDFVIVHDPQPLAMRHFLPDHDGKWIWRCHIDTSSPHPGV
                     VELLEPYVEEHDALVFTLPQFAPDAWDHSVIRTMAPGIDPLSPKNMDLPPQVHDQIAR
                     WLAVDPRRPVMTQVSRFDSWKDPLGVVEVFRTVRQEVEGLQLALLGSMAMDDPEGWGL
                     YERIREEAGGDRDIHVATNINGVSNIEVNAFQRLSDVVIQKSIREGFGLVVSETLWKE
                     TPVVAGRAGGIPVQMPEGTGGVLATTDEEYAEGVLRMLHEPELARDLAERGREHVRER
                     FLITRLVADELRLLLELR"
     gene            complement(5428..6219)
                     /locus_tag="VF097_01025"
     CDS             complement(5428..6219)
                     /locus_tag="VF097_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6261407.1"
                     /translation="MDLGLKDRPALVAAASKGLGRACAAVLAEEGARVAICARDAGAL
                     EATRDEIAEATGATVVAVPADVSTEAGAVSFVREGAEALGGCEILVSNVGGPRTGTFD
                     ELEDEDFENAFELLFLSTVRMTREALPHMRAAGYGRVVIIGSSAVKQPIPGLMLSTSI
                     RTGLIGWARTLVDEVGPEGITVNAVLPGRHATDRIRELHEDRARREGVSPAEVAAEDA
                     SQIPLRRIGDPRELAHVVAFLASERASYVSGAFVQVDGGMYRGLL"
     gene            6247..6588
                     /locus_tag="VF097_01030"
     CDS             6247..6588
                     /locus_tag="VF097_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX6261408.1"
                     /translation="METTRSVLSVTPEAAAKARTLAEREGRTEAWLRVRVVAGGCSGF
                     SYKLSFEDVAAENDHVVEEHGLRVIVDPASIPILEGSTLEFRDALLGGGFKIENPRAQ
                     HECSCGESFSV"
     gene            6614..7738
                     /locus_tag="VF097_01035"
     CDS             6614..7738
                     /locus_tag="VF097_01035"
                     /inference="COORDINATES: protein motif:HMM:NF016328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HEX6261409.1"
                     /translation="MAKDEPKVQWRELFDEVVATGLCTGCSACVVVCPFHVLGYEDHK
                     PIQLEEGPANACTHGDNGCSICTRACPRFREWEPAIDELMFGRRRLPEEAIGVHREIL
                     LARATEPAALASGQDGGVVSALLLWGLRTGQIDGALTSRLSDERPWDAEPTVVTTEEE
                     VLATAGSRYTYSANPLALTAAMQMGLKQVALVGMSCQASVTGAMEAGRLNKYRRRIAW
                     TFGLLCSKTFTYEGLNEEIIQERLGLSLDDVVRVNIKGKLLVYTRDGTEHTFSLKEAQ
                     EFTRPGCLKCPDFAAEHGDISFGGLGQEGGWTLTVLRSELGADLWARAKRDGVIEDRP
                     ASEDPKAVTLMERLAARQRGRWPVEELPAEWAVPGVAPSD"
     gene            7843..>8502
                     /locus_tag="VF097_01040"
     CDS             7843..>8502
                     /locus_tag="VF097_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016116112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6261410.1"
                     /translation="MAEISLDGVYKIYPDGTEAVRDFSLDIEDGEFMVFVGPSGCGKT
                     TALRMVAGLEDISKGTVLIGGREVNDVPPKDRDVAMVFQNYALYPHMSVAANMGFGLK
                     LQKVPQDEIDRRVTDAAEILGLSEFLKRKPRALSGGQRQRVAMGRAIVREPQAFLMDE
                     PLSNLDAKLRVQMRAEIAKLQNDLGTTTIYVTHDQIEAMTMGDRVAVIKKGELQQVDR
                     PQ"
BASE COUNT         1259 a         3030 c         2898 g         1315 t
ORIGIN      
        1 gagccgacgg caacgtcggc gcgaggaggc agcgcctgct gcggctcccc gaagtctcgc
       61 cctaccacga cgggggggac atcgcgttcg gccctgacgg gatgctgtac gtcgtgacgg
      121 gcgaggccca cagccccgag cgggcccagg acccggagga cgtcgcgggg aaggtgctgc
      181 gcctcgaacc cgacggctcg atccccgaca accccctccc cggccccgtt ttctccatgg
      241 gccaccggaa ctcgttcggc gtgtgcgtgg acgccggcac cgggaacgtc tggcagaccg
      301 agaacggccc cagcgccaac gacgaggtga acctgatcga ggccggaggc aacttcggct
      361 ggccggccgt ccagggcgcc gcggacgacc ctcgcttcgt ggaccccgag atcgacttcg
      421 tgaacgtggt cgtgccgacc gggtgcgccg tctacccgca cgaccacctc gggccgcgga
      481 gcgagggggc gctgttcttc ggggactacg gcggaaccct gtggcgggcc gtcctggacc
      541 agggaggggc gggggtggag cggcaccacc ggttcatgac cggcctgggc ggcataaccg
      601 acatcctcgt gggccccgac gagcgactgt acgtcgtgac gaacgagagg atcctgcgcc
      661 tccctgccga gatcccgagc ccggagccct cgccctcttc cccgccgtcg cccggcacgt
      721 cgtccgagcc cgagcccacg atcgacatcc ccctggtgcc atcgtcggag gacggcggga
      781 tcccctggct cctcctggcc ggcgtcggca tcctcatcgg tgccctcgcc gcgatgtggt
      841 tcgcgcggcg gggcccgacg tgacggggct cagccctccg tggggcgctt gaagtaggcg
      901 atcagcccct gcggctggga caccacgctc acgagctccc acccgtcctc gccccactgg
      961 ttcaggatct cctgggtggc gtgttcgatc agcggggcgg tagcgtactc ccaggtcgtc
     1021 atcccgcgac ccccaggcgc gcgtgggtct tcttgatgca gatccccatc acgacctcga
     1081 ggccaccgcc ctcggcgatc tgccgggcct catcgctcac gatcccctcc tggagccaca
     1141 gcaccttggc ccccacgtcc accgcgtcct tggccacggg cggcgtgttg tcggcccgca
     1201 cgaatacgtc cacaacgtcg atcggctccg gcacctcgtg gagcgagggg tagaccttct
     1261 cgcccaggac ctgttcctcc tccttggggt gcacggggat gatccggtac ccctgctcct
     1321 tgaggtacgc cgccaccccg tacgcggggc ggtcggtgcg gttcgagagc ccgaccaccg
     1381 cgatcgtctt tccctcgccc aggatgcgct tcagctcctc gtcacccggg tgaaagggcg
     1441 tcacatcgtc catgtccatc cctcccgttc cagcgtccgc aggaggtggt ggaggatccg
     1501 ccccgtgagg ccccacacca cctggccgtt cacctcgtag acgtacgtat cgtaggagcc
     1561 accttcccac acgacgcgct ccagccgctc gacggccgcc agcagctcca gcgggagctc
     1621 gaggacctcg gccacctcca tggggttcgg ccgcagctcg ggccgctcac ccagatgggc
     1681 tacgaatggg accaccgcgt agcccgacac gccggcctgc atcgggacga ggcgccccag
     1741 cacgtcgtgg gcggaggcgg ggatccccag ctcctcctcg gtctcgcgga gggcggtggt
     1801 cagcaggtcc gggtcctcgt cgtgccgcgc gccccccggg aacgagacct ccccccgatg
     1861 gtggcgcagg tcgtcggtgc ggcgcgtgaa cagcagggcg ggctccggcg cctccagcag
     1921 ggggagcacg accgccgccg gcacaccttc cggtagcggg gggtcgggcc gtggaggcgg
     1981 cagggcgccg cggagcatct cggcgaggtc ggccatccct cgatggtagt gcccgggaac
     2041 gtcgagaggg gggcccgagg ccccctctcg atgacgctcc tcgctcagag ctgcttgatg
     2101 atgagcttgt acggcccctt gaacttcagc caggccgtca cgtggaagaa gtacctgccc
     2161 cccttggtgg ccttgaaggt gacgacttcg ttcttcccct tgcccttgaa gctggccctg
     2221 accaccgggc acttcctgcc gccggagaag caggccggct cgaactgcca gatctgcttc
     2281 gtgcccggct tccagaccca gaggtcgaag tcggacttgc ccacgcggag cgtgacgcga
     2341 taccgcttcc ccttctgcaa ccttcgcttg tagaggtcgt tgacgtcgag ggggtaagcc
     2401 acccgcccct tcttggaccc ggcgatcctc ttggcgccgg ggatgtcccc gtcggtcttg
     2461 ggcgtggcgt tgcccgtccc cgccgcgagg gcgccggcgg cgtcgagcct gccgctcgtc
     2521 cgggtgaact tcccgctcgc ccgtcccccc ctcaggatgt aggagtagaa cgtcgccagc
     2581 gaggccaggt tcttgtccgc cccgttcatg atcttgttct tgacctgcac ggggctgagc
     2641 cccgggaact ccgagcgaac cagcgcggcc gcccccgcca cgtggggcga cgccatcgac
     2701 gtgccgttga actcgtcgta ggccgtgtcc cccgtcttca ccgagctcct gatgtcgacg
     2761 ccgggcgccg cgaggtccac cgactcgcga cccacgttcg agaacgagca ctcggccttg
     2821 ctgaacccgt cccccggcga cggcgcgttg cagcccgtga agtacccgta ctcgtcgtgg
     2881 tggttgctcg cggccaccgc gatgatgttc ggcaggttgt acgacgccgg gaaggccggg
     2941 ctgatacccc ccgtcactgg gttcccagcc gccgtgtcgt tgtccagcga gtcgttgcca
     3001 gcggcgaaca cggccacgat ccccgcctgg ttgagctgct tgatcgcgtt gtaggacacc
     3061 ttcgaccaga ccgccccgcc aaaggagccg ttgatcaccc gggccccgtt cttcctggcg
     3121 tacgcgtagg ccgcgacctc gccggcggtg gtgaggtcga actttgtcac catgatcctg
     3181 cagtgggggc agaccccgat gatcccctgg gcgttctcgc cctcggccgc gatcgtcccg
     3241 gccacgtgcg tcccgtggcc ggcgccggac gcgtcgtcgc tgaggttcga gcagagggcg
     3301 ggcgtgaacg agcacccgtg cacgtcgtcg acgtacccgt tcccgtcgtc gtcgaccagg
     3361 ttcgccgggg tctctccgcc gttgacccag aggttgttgt ccaggtcgtc gtgggtgatg
     3421 cggacgccgc tgtccaccac cgcgacgatc gggtcggtat tgtccccgtc ctcggtgacc
     3481 caggccgcgt tcgcagcgat gtcggcgccc gatgtcccgg aggccgtggt cgggggcgga
     3541 tcgctgatgt tgtgagcctg gcctgtgttg cccaaccccc acagatgggg gatgtcgggg
     3601 tcgtccaccg gaggcgtgat gtggctcagc cactggtact tgaagttggg ctctgccacc
     3661 acgacgccgg gatccttctg gtagcggcgg gcggccttcg ccaccgagac gccggccggc
     3721 agcttcacgt gctcgatccg gcctcccagg cccgtgacgc ggttcagcac ccgggcgccg
     3781 ccgtgggcgg cgtggatctg catcgccgcc gggcgcttca cgcccttctt gaactgcacg
     3841 accagctcgc cctcgacgaa gaggtcggag gggccaccca ggtttccggg ggtgcgcttc
     3901 accgcctggc cgatctccgg cggcacgaag aagccccgag cctggttgga gcccgcggtg
     3961 cccgccgacc ccggtgcggc gaacagcgcc ccggacagca ggagaacgga tacgacggtc
     4021 gagaccacag aacgcttcaa agagatcctc cctctcctct gccggcgcca agcgccgccc
     4081 ggaagacacc ttggcagcgc gcgacacgat acgcgcttcc ctgaagctct tcaatggtca
     4141 ttttcgtgct ccccggtccc ccatctgccg gacagggtcc cggttggggt cagccctatc
     4201 tgagctcgag caggagcctc agctcgtccg ccaccagcct cgtgatcagg aagcgctcgc
     4261 ggacgtgctc gcggcccctc tcggccaggt cccgcgcaag ctcaggctcg tggagcatgc
     4321 ggaggacccc ctccgcgtac tcctcgtcgg tcgtggccag gacgcccccg gtcccctcgg
     4381 gcatctgcac cgggatgccg ccggcgcgtc cggccacgac gggggtctcc ttccacaggg
     4441 tctccgagac gacgagcccg aacccctcgc ggatcgactt ctggatcacg acgtcggaga
     4501 gacgctggaa cgcgttcacc tcgatgttgc tgaccccgtt gatgttcgtg gccacgtgga
     4561 tgtcgcgatc cccgcccgcc tcctcgcgga tgcgctcgta gaggccccag ccctcggggt
     4621 cgtccatcgc catggagccg aggagggcga gctggagccc ctcgacctcc tgccggaccg
     4681 tccggaagac ctccaccacg ccgagcgggt ccttccagga gtcgaagcgc gagacctgcg
     4741 tcatcaccgg cctccgcggg tccaccgcca gccaccgcgc gatctggtcg tggacctgcg
     4801 gcggaaggtc catgttcttc ggggagagcg ggtcgatccc gggcgccatc gtcctgatca
     4861 ccgagtgatc ccaagcgtct ggagcgaact gcggcagcgt gaacaccagg gcgtcgtgct
     4921 cctcgacgta gggctcgagc agctccacga cgccggggtg ggggctcgag gtgtcgatgt
     4981 ggcatcgcca gatccacttc ccgtcgtggt ccgggaggaa gtggcgcatg gcgagcggct
     5041 gggggtcgtg gacgatcacg aagtcccagt cgccttccag cgcggcggcg ttctgctcgc
     5101 tgtgcctcag gtactcgctg cggccctcct cgccaagctc gaagtcgcct ccctggaggg
     5161 cgttgtggaa ccccttcgtc acctcgaaga aggcatcgtc gccggtgatg acctgccact
     5221 ccgcctcgat gcccaggcct cgatagatgg gcagcatcga gcgcagcagc tcggagaccc
     5281 cgccgccgta ggccgtggcg ttgagctgga cgaccctggc gccacggagg gagcgcccca
     5341 gctcgaccag ctcctcgacg gcctcgcggc cgatcatggg ctcgtaggcc tcgatcgact
     5401 gggtctcgac gtcgacgacc tggagcatca gagcagacct cggtacatcc cgccgtccac
     5461 ctggacgaag gcgccggaga cgtaggatgc ccgttcggag gcgaggaagg ccacgacgtg
     5521 ggccagctcg cgtgggtcgc cgatccggcg gagggggatc tgggaggcgt cctcggccgc
     5581 gacctcggcg ggtgagacgc cctcgcgccg ggcccggtcc tcgtggagct cgcggatgcg
     5641 gtcggtggcg tggcgcccgg gcaggacggc gttcacggtg atcccctccg ggcccacctc
     5701 gtccacgagc gtacgggccc agccgatcag cccggtccgg atcgaggtgg agagcatgag
     5761 gcccgggatc ggctgcttca cggccgagga accgatgatc acgaccctcc cgtagcccgc
     5821 ggcccgcatg tgcgggaggg cctcccgggt catgcggacg gtggagagga acagcagctc
     5881 gaacgcgttt tcgaagtcct cgtcttccag ctcgtcgaag gtgcccgtcc tcggcccgcc
     5941 cacgttggag acgaggatct cgcatccgcc cagggcctcc gccccctccc ggacgaacga
     6001 gacggcgccg gcctcggtgg agacgtcggc gggaacggcg acgacggtgg cgccggtggc
     6061 ctcggcgatc tcgtcccggg tggcttcgag ggcgcccgcg tcgcgagcgc agatcgcgac
     6121 gcgggcgccc tcctcggcca ggaccgccgc gcacgccctc cccagcccct tgctggcggc
     6181 cgccacgagc gcgggacggt ccttcagacc cagatccacg gtcccgttga tatcatcaga
     6241 ggcgagatgg agaccacccg aagcgtcctg agcgtcaccc ccgaggcggc ggccaaggcc
     6301 cgcaccctcg ccgagcgcga gggccgaaca gaggcctggc tccgggtccg agtggtggcc
     6361 ggcgggtgct cggggttctc ctacaagctc tccttcgagg acgtcgcggc cgagaacgat
     6421 cacgtcgtgg aggagcacgg cctccgcgtg atcgtggatc ccgccagcat cccgatcctg
     6481 gagggctcca cgctggagtt ccgcgacgcg ctcctgggcg gcgggttcaa gatcgagaac
     6541 ccgcgggccc agcacgagtg ctcctgcggc gagtcgttct ccgtctgagg cccccgccta
     6601 tacactggcc gcgatggcca aagacgagcc caaggtccag tggcgcgagc tcttcgacga
     6661 ggtcgtggcc accggcctct gcacgggatg ctccgcctgc gtcgtggtct gccccttcca
     6721 cgtcctgggg tacgaggacc acaagccgat ccagctcgaa gagggtccgg ccaacgcctg
     6781 cacccacggc gacaatggct gctcgatctg cacccgggcc tgcccccggt tccgcgagtg
     6841 ggagcccgcg atcgacgagc tgatgttcgg acggaggcga ctccccgagg aggcgatcgg
     6901 ggtccaccgc gagatcctcc tggccagggc caccgagccc gcggcgctcg cctccggcca
     6961 ggacggcggg gtcgtctccg ccctcctcct ctgggggctc cggaccggcc agatcgacgg
     7021 ggcgctgacg tcccggctct cggacgagcg gccctgggac gccgagccca cggtcgtcac
     7081 caccgaggag gaggtcctgg ccacggcagg gtcccggtac acgtactcgg ccaacccgct
     7141 ggccctgacc gccgcgatgc agatggggct gaagcaggtg gcgctcgtgg ggatgtcgtg
     7201 ccaggcgagc gtcacgggag cgatggaggc cggccgcctc aacaagtacc gccggcgcat
     7261 cgcctggacc ttcggcctcc tctgctccaa gaccttcacc tacgagggcc tgaacgagga
     7321 gatcatccag gagaggctcg ggctgtcgct cgacgacgtg gtccgggtca acatcaaggg
     7381 gaagctgctc gtctacacgc gtgatgggac cgagcacacc ttctcgctga aggaggctca
     7441 ggagttcacg cggcccggct gcctgaagtg ccccgacttc gccgcggagc acggggacat
     7501 ctcgttcgga ggcctgggac aggaaggcgg ctggaccctc accgtcctgc ggagcgagct
     7561 cggcgccgac ctctgggcga gggcgaagcg cgacggcgtc atcgaggacc ggccggcgtc
     7621 cgaggatccg aaggccgtga ccttgatgga gcgcctggcg gccaggcagc ggggccgatg
     7681 gcccgtggag gagctgcccg ccgaatgggc ggtcccggga gtcgcaccgt ccgactgagc
     7741 ggcccatcct tccccgttcg gggcgaaagg aaacttcaca ctagcggccc ggatgtggta
     7801 ggtgataggg gcacttctgg cgacccgatg gggggtgcaa gggtggcgga gatctctctg
     7861 gacggggtct acaagatcta ccccgacggc accgaagcgg tacgagactt ctccctcgac
     7921 atcgaggatg gggagttcat ggtcttcgtg ggcccctccg gctgcgggaa gacgaccgcc
     7981 ctccgcatgg tcgccgggct cgaggacatc tccaagggaa cggtcctgat cggcggccgc
     8041 gaggtcaacg acgtcccgcc caaggaccgt gacgtcgcga tggtcttcca gaactacgcc
     8101 ctctaccccc acatgtcggt cgcggccaac atgggcttcg gcctgaagct tcaaaaggtg
     8161 ccacaggacg agatcgaccg tcgcgtcacg gacgccgccg agatcctcgg acttagcgag
     8221 ttcctgaagc ggaagccgcg cgcgctgtcg ggcggccagc ggcagcgggt ggccatgggc
     8281 cgggccatcg tccgggagcc ccaggccttc ctcatggacg agcccctctc caacctggac
     8341 gcgaagctcc gggtccagat gcgcgccgag atcgccaagc tccagaacga cctcgggacc
     8401 accacgatct acgtgacgca cgaccagatc gaggcgatga cgatgggcga cagggtcgcc
     8461 gtcatcaaga agggcgagct ccagcaggtc gacaggccgc ag
//
LOCUS       DASUTZ010000022        15645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_394173, whole genome shotgun sequence.
ACCESSION   DASUTZ010000022 DASUTZ010000000
VERSION     DASUTZ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 15645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15645
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394173"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VF097_01045"
     CDS             complement(<1..549)
                     /locus_tag="VF097_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012810694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6261411.1"
                     /translation="MRILILGGDGYLGWPTAMHFSQRGHEVHTVDSYLRRRAHAEAGT
                     DSLTPILGSLPERARAWEEATGKHIGVTEGDVCDWETLERVFLTFRPEAIVHYGEMPS
                     APYSMRDRAHAVFTQYNNVIGTLNVLFAMRDITPDAHLVKLGTMGEYGTPDIDIEEGF
                     FEVHHKGRSDTVPFPKQPGSLYH"
     gene            640..2490
                     /locus_tag="VF097_01050"
     CDS             640..2490
                     /locus_tag="VF097_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6261412.1"
                     /translation="MHPRTLFVAAWAVPAAALVASAVAAFATGWIAAAVVGVAVLVLG
                     AALVASLRPRSTDLGRRRVLAGLGIAGIGAVAGGSAVGRALSVLEPELAPGPVLRGMA
                     RRLGGPAMRSVLRGYHPERSGDLQMILQPFNTSNYPHESRSLVPDDPRSSHSLVWGYT
                     DRVPIVVYAPNLVEPQDRTDEATLADLAPTTARLMGLDVSFPDGEPLPGIEAPARPPK
                     VIVTCVIDGGGWNVLQKWPDAWPMLARLARDGALYRNGYMGSFPNVTASAHATIGTGA
                     FPRAHGISGHHVRAGRGTQPALGELGAADPSRILVPTLADVWDEETGGRAWIGEIGYQ
                     VWHLGMIGRGGERPVAVYWDEAGNDWAVQNPELYRMPGEVPPRAALTDRMRRYFGPER
                     GAARDREGGKAICCDPPIVEHQGDLLEATLRVEPIGETGVTDLLYVNYKAPDYAGHVS
                     NMLDPHERVALEAVDRDLERLVAVLERRFRPGEWALILTADHGQCPLIEEAGGVRLDL
                     VQLSEDLEGELDGGTVVGIRPSEVFLDADRAAVERLATSIAGYRYGENVGPYVPEEAV
                     LEDRMDRRHFAAVLPGSFIRGLDEAEVAAFGPGRFAQADPGVPPRSLVEG"
     gene            2493..4199
                     /locus_tag="VF097_01055"
     CDS             2493..4199
                     /locus_tag="VF097_01055"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6261413.1"
                     /translation="MARAAGALAALLIVAAGCTGGPEDPVPDDASTIPEMLRALGADP
                     VRQLARGYVPERSGDVVLVPAPGSTVAQWPGGLRSTTDPRTTHGAPWDYIARVPIILR
                     GPGHVPAGAVPDRPVDVADIAPTIADLLAFDWEAPDARSLEEALLPDRDPPRVITLVV
                     HDGGGWNVLERWPEAWPFQRGLAAEGTTFGNATVGSAPPITAPIHANMGTGTYPRTHG
                     IAENTGRLPDGSLGELFFHEADPRLMEAETVADAWDVAQGNEAWVGLVGYEAWHLGMM
                     SRGTRHPGGDRDVAVLWDHRETDDLFTNEEVYRMPEYLRGREALEERVHELDLADGAA
                     DRRWMQADLDDIFFIPATPAFVEYQTDLVLEMIEREGIGTDDVTDLLFVELKPSDNAG
                     HLWNMESPMFEEVLRAQDRAVERIVGALDERVGPDRHVVIVSADHGQSPRPDVVEGLR
                     IDRFRVQEALNDAFGAEVVEAVHPDDLYLRWDVLGEIGATAEDVARAAASLRFDQVAP
                     QGTDLEALSEELAAARPFAAAIPVRRLVGLSEEEVAALGAGAFPEADLTSPPDLARLL
                     RR"
     gene            complement(4189..4887)
                     /locus_tag="VF097_01060"
     CDS             complement(4189..4887)
                     /locus_tag="VF097_01060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEX6261414.1"
                     /translation="MRRLSPTRVTVGLALAAWAGVFWFLLVTDRWTLYLSSRIFWVVP
                     VGAVLLTATALGRLLTARTERAEPLAVRDTWGLALVLVPVVAVLALPPTALGSYAASR
                     RSVSAGFVTAAGEPSSGEVTLVDVAGAMWSQTAADSLTERAGSEVSFVGFVVRREGTP
                     ADEFVLTRFLVSCCVADALSVQVRVVGAPPGAFEEDEWVRVQGTLYPLPDQVVVDAAS
                     VKAVPRPAQPYLNV"
     gene            complement(4884..5894)
                     /locus_tag="VF097_01065"
     CDS             complement(4884..5894)
                     /locus_tag="VF097_01065"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEX6261415.1"
                     /translation="MAVIEKPRLRLPGERRPAWRGGLVLLLVLIASGTILRLLDAAGL
                     PPVRNFLLVWASLLVEAVPFILLGAIVSAAIEVFVPARLFARLARLPGPVQMPVAGLG
                     GFVFPVCECGSVPVARRLVVKGLTPAAAITFMLAAPILNPVVLVATAVAYRGRDVLWV
                     MVGGRAILGLVVALAVGWVVARKGKRELLRAPEDHHEPGEPLARRWPAYFAHLSSDFV
                     MLGRYLVIGAGVASALQTFVPQGIVGAVAETPVLNILAMMTLAGILSLCSESDAFVAA
                     SFVQFGAGAQLAFLVFGPMVDAKLGFLYVGTFSRGFLRTVVAVVGAVTLAGALWVEVL
                     AG"
     gene            complement(5894..7237)
                     /locus_tag="VF097_01070"
     CDS             complement(5894..7237)
                     /locus_tag="VF097_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261416.1"
                     /translation="MTSIPPPSFASGLGRRTLVIVAAAGLLAAPALLLRAFCVGESCR
                     RQAAPEVRAPFCSLDPQLRRLLADGYRQGRSPEVLVVTSEDATVAGATGLEDLSAPWP
                     SVEDVDAGRVPLVFQGAGIDPAAEVPAATPLVDLAPTAAAILDVRVPHPGVRSGEAIP
                     GVAAGGPARLVVLVVWKGVGSDDLEASPAAWPTLRRLLREAAGTLDARVGSLPLDPTA
                     VLTTIGTGGLPREHGITGSTLRNEEGKAVPAWGRGAPYSVIAALGDDLDEVLGQRPLV
                     GLVGASALDRGVIGKDWHLRTDQDEVVLKDRAVRQADAAADLLAGGYGADDVPDLLAV
                     VMHDRVPAMDRALQRVVQAVPPSTAVVVTSTGPLAAEEAVGADRIRAEVEGAGAGLTE
                     AVAVGGLFLDQDVLVEADASQEDVIRALRGIRASIGGPMFADVFGGRAVEFGRYC"
     gene            7286..8980
                     /locus_tag="VF097_01075"
     CDS             7286..8980
                     /locus_tag="VF097_01075"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6261417.1"
                     /translation="MMRALVLVVAVLSASCSGPPDPPPGARSVEEMAREVGADVLEAM
                     RRGYVPGRSGELALIPRPHHVIVRRGSWDPGGEPSPDTSHSTPWAYHQRVPILLYGPG
                     FVREGTVSERAVDLADLAPTLAELMGFPFRARDGVPLSEALAPGQRGAPRAIALVAYD
                     GGGWNLLQAYPGDWPFLRALMARGALFTNATVGSSPSVTAAVHPTMGTGAYPAAHGVP
                     DNSARLPDGTVGDAYLGRTDPRLLRVETLADAWHEATGAWTGIVATESWHLGMLGRGA
                     GPGAAPEQVAVLWNRRERRFFTNPERYRLPGYLPAEDSLEARLRELDGSDGSLDGRWM
                     GNDLEDPGVLPGTPAFVRYQGDAVLRILGRERIGRDDITDLLFVELKSTDRGAHLWNM
                     VGEEQPHVLRAQDDLLRDVAAALDAEVGEGRWVLAVTADHGLTPLPEEVGGVRLHPDA
                     LAAAVEERLGPIVEAATPSSLFLDMDEVRSRDLDLDLVATIVGDLRYRDGIPEGVDLE
                     RIPGDLLDRRVFAAVLPGPYLEGLTAEETARAGLGIYEEGDLTGVDAAARFARLAG"
     gene            complement(8934..10271)
                     /locus_tag="VF097_01080"
     CDS             complement(8934..10271)
                     /locus_tag="VF097_01080"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6261418.1"
                     /translation="MSIARDSLALFRKRDFTFLMGAQWLAQAADGLVGLALAKKITFG
                     GSAGFDLDVEGAATPREALLIVLLTFLPYMIISPFMGVFIDRWNRRMLLILANGVRAV
                     LLLTVGILGPDAIGNVALYGSFLLVLGGTRLVLAIKGAGLPAALGGEDELMQGNATSQ
                     AGSAIFQLGGAGVALVAAGFVDARLIILAGALVYGAATASASLVHRLGEARAVVPLRK
                     EIGRVFRDLLDGFREIGRRPMAGLAIVSFLSVRTLLTLVVLATVFISRDLIASQDTLT
                     TAIPAAAGALGAVVGFVIAHALKDRVRPARIVSGALLFGAAGMLAFGGVINMLGISAL
                     AFCVGVAFFLGKVAVDTLMQQALADSYRGRGFSLQDVSYNLSWVLPALLLWLVLTPDT
                     ARMVLIGSGAAFLLIALLIGLWSRALGGRPERKIEAEEVSPPASRTSPPRPPP"
     gene            10323..11942
                     /locus_tag="VF097_01085"
     CDS             10323..11942
                     /locus_tag="VF097_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6261419.1"
                     /translation="MRRVTRSVAALGVAVLFAASCTDAGPEGLRAPPTAVPTPAPPTA
                     LPAEASPACSLPPEHLLRIRRGYHPERSGEIQIVPRLPAVVASWLSHAGPWDHVQRVP
                     LFLYGPGHVPAVGRVDRPVTLADLAPTMARILGSDFEAPDGRPLREAVAGSDPPRLIL
                     TVIWDSAGRNVLDEHPEAWPTLRALIPQGTWFEEATVGTSPSTTPAVHATIGTGVFPS
                     RHGLVDLHFRWGGRMAHILDERPAFLSMPTLADRYAANTGQAAKVGLIASLGTLGLIG
                     HGASIEGGGRHLVAMHHEGGWGIGEAYAEDFRFPAYLRDVGGFAASLRRLDLEDGAAD
                     GRWLGEPVLRPGEDRSISPAWTEHQTRVLEEVVEREGFGRDAVPDLLFTNYKQIDSVG
                     HRWGMHGPQMEAVVRSSDTALRTLIAILDREVGEGEWVLALTADHGSIPDPAGAPIID
                     LRELRRDLAEAFGDAVEQVRPTQVWVDERALASRGATLEDVAELLGGYRRSDNHELPP
                     GEPDTRLFAAAFPSEVLLTASCVPSQRGAFG"
     gene            12286..13620
                     /locus_tag="VF097_01090"
     CDS             12286..13620
                     /locus_tag="VF097_01090"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6261420.1"
                     /translation="MPLLFYGPGHVPEGVRVSRTATLADVAPTQAALLGFDFDAPDGE
                     PLAEAVAGAERPPKLIVTMIWDSAGMNVLEEHPDAWPTLGRLIERGTFFEETFVGSSP
                     SVTPAVHATIGTGAFPRNHGIVDLRFRVDGVPTAARGKAAANLLVPSLSDLFDVARDN
                     EPVVGLIGSEGTLGMLGHGAFWESGDSDVAVTRTRTEWTLEGLNAEAFAFPEYAGGFP
                     GLEEAIRRLDLEDGQADGRWLGEPMDEGVEGVSATPAFGEYQRSMVREVIEREGFGRD
                     EVPDLLYVNFREIDAVGHRWGMHGPQMEAVVRSTDEALADLVRTLDREVGAGEWVLTM
                     TADHGATPDPEPLGGFVIGPQEVKKDIEAEFGPIVGDLRVTQIWLELEEMAERGVSLQ
                     EVASFVADYRRSENASDPASLTAEQGEERVFGTVFPSAVLDDPPCIITTRSG"
     gene            13633..>15645
                     /locus_tag="VF097_01095"
     CDS             13633..>15645
                     /locus_tag="VF097_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261421.1"
                     /translation="MKTERAREDATASERWGPLGGPIAWAGRVAAHPGTALAAVAAIA
                     LLMAWQFIVDASRAVPALDTAWYQWRAEYIQANDPGSLVGIQGADGSLAGGYRVAEPV
                     LAALLRIVGGVGAEVPTVVLSVFFRAAAAVALAAFAWRHRRSRLLFYLTLAVVPALFL
                     LQGFFGYLDNFFALALTGAALLLLEPMRTSWVARLVVIAMLFLMGLTHPTTLALFLLA
                     LGAVAGYQFLREGLSIRSALRSDVMPMLWAGVVSVVLMFAAWLGGMWGPSAGLNEAAV
                     PPPQPVSYFVDRSINVFRGMQPITLVPLFLIGFVALAVDAWRRRERLAEITVAWDLPL
                     LGMLGFLLGAAYPYFRFFNATLAPLLTIAVGFAVIIGAASRLRREGTARLASVVAVAV
                     TVGVLASWWIGGLSSWNSKSTWLTSEVRTSLGAISGYLEGEPAGRKAVVVVDAQPNGV
                     VPYSRYKEFANAVYAGIPGDRIDDTELFFGRIEDLEAGTTTSVGDEQYDTMARDSAAE
                     ALPLLEQEAVVLMPLVFNEPSDNAAFLEGCAECRELGDSEVYAIPELSTAALSEEAVA
                     AGAEAADQARAFAESPPGPFEGLGSSLLSLLGLLLLLAVPGWLLLRGIPGRDPIEAIA
                     LVPMLSVTLVTTVGVVALGIIRGPRTSLVGWIVWAVSVGVAAWAAFG"
BASE COUNT         2155 a         5617 c         5775 g         2098 t
ORIGIN      
        1 gtggtagagc gacccgggct gcttggggaa cggcacggtg tcgctccgtc ccttgtggtg
       61 gacctcgaag aagccctcct cgatgtcgat gtccggcgtg ccgtactcgc ccatggttcc
      121 gagcttgacg aggtgggcgt cgggggtgat gtcgcgcatc gcgaacagca cgttcagcgt
      181 cccgatcacg ttgttgtact gcgtgaacac ggcgtgggct cggtcgcgca tcgagtaggg
      241 ggccgagggc atctcgccgt agtgcacgat ggcttcgggg cggaacgtga ggaagacgcg
      301 ctccagggtc tcccagtcgc agacgtcgcc ctcggtgacg ccgatgtgct tcccggtggc
      361 ctcctcccag gcccgggccc gctccggcag cgagcccagg atcggcgtca gcgagtcggt
      421 cccggcctcg gcgtgggccc gccggcgcag gtagctgtcc acggtgtgga cctcgtgccc
      481 gcgctgggag aagtgcatgg cggtgggcca gcccaggtag ccgtctccgc cgaggatcag
      541 gatgcgcatg ccgcgtgcct ccaacagtgg ggaacgcctc attgtagtcg tcgggcccgg
      601 gcccgggacg tgtccgggtt gttctgcgac aatcggctcg tgcacccccg aaccctcttc
      661 gtggccgctt gggccgtgcc cgcggctgcc ctcgtggcct cggccgtggc cgcgttcgcc
      721 accggctgga tcgccgccgc cgtagtcggc gtcgcggtgc tggtcctggg ggcggccctc
      781 gtggcctcgc tccgtccgcg ctccaccgat ctgggccgcc gccgggtgct cgccggcctc
      841 gggatcgcag ggatcggcgc cgtggcggga ggctcggccg tcgggagggc cctcagcgtg
      901 ctggagcccg agctcgcgcc ggggcccgtc ctgcggggca tggcgcgccg cctgggcggc
      961 cccgcgatgc ggagcgtgct ccgcggctac cacccggagc ggtccggcga cctccagatg
     1021 atcctccagc cgttcaacac ctcgaactac ccgcacgagt cgaggagcct cgtgcccgac
     1081 gacccgcgca gcagccactc gctggtctgg ggctacaccg atcgtgtccc gatcgtcgtg
     1141 tacgcgccca acctggtcga gccccaggac cgcaccgacg aggcgacgct ggccgacctg
     1201 gccccgacga cggcgcgcct gatgggcctc gacgtttcgt tccccgacgg cgaaccgctg
     1261 ccgggcatcg aggcccccgc ccggccgccc aaggtcatcg tgacctgcgt gatcgacggc
     1321 gggggatgga acgtcctcca gaagtggccg gacgcgtggc ccatgctggc tcgcctggct
     1381 cgggacggcg ccctgtaccg gaacggctac atgggctcgt tccccaacgt cacggcctcg
     1441 gcccacgcca cgatcgggac cggcgcgttc ccccgggccc acgggatctc cgggcaccat
     1501 gtgcgggccg ggcgggggac ccagccggcg ctgggggagc tgggcgccgc ggacccgtcg
     1561 cggatcctcg tccccaccct ggcagacgtc tgggacgagg agacgggcgg ccgggcctgg
     1621 atcggcgaga tcggctacca ggtgtggcac ctggggatga tcggacgggg aggagagcgg
     1681 cccgtggccg tctactggga cgaggcgggg aacgactggg ccgtccagaa ccccgagctg
     1741 taccgcatgc ccggcgaggt gccgccccgc gcggccctga ccgatcgcat gcgtcgctac
     1801 ttcggccccg agcgcggcgc ggcgcgcgat cgcgaaggcg ggaaggcgat ctgctgtgac
     1861 ccgccgatcg tcgagcatca gggcgacctg ctcgaggcga cgctgcgcgt ggagccgatc
     1921 ggggagaccg gcgtcacgga cctcctctac gtcaactaca aggcgcccga ctacgcgggg
     1981 cacgtctcga acatgctcga tccccacgag cgcgtcgccc tcgaagcggt ggatcgcgat
     2041 ctggagcggc tcgtggccgt gctcgagcgg cggttccgtc cgggggagtg ggcgctcatc
     2101 ctcacggccg atcacgggca gtgcccgctg atcgaggagg cggggggcgt ccgtctcgat
     2161 ctcgtacagc tgtcggagga cctggagggg gagctcgacg gcggcacggt cgtcggcatc
     2221 cggccgtccg aggtcttcct cgacgccgac cgcgccgccg tcgagcggct ggccacctcc
     2281 atcgccggct accgctacgg cgagaacgtg gggccgtacg tccccgagga ggccgtcctc
     2341 gaggatcgga tggaccgtcg ccacttcgcc gcggtgctcc cgggctcgtt catccggggc
     2401 ctcgacgagg ccgaggtcgc cgcgttcggc ccggggcggt tcgcgcaggc cgatccgggg
     2461 gttccacccc gaagcctggt ggagggctga cggtggcccg agccgccggg gccctggccg
     2521 ctctgctgat cgtggccgcg ggatgcacgg gagggcccga ggaccccgtc ccggacgacg
     2581 cgtcgacgat ccccgagatg ctccgggcgc tgggtgccga ccccgtccgt cagctggccc
     2641 gtgggtacgt ccccgagcgc tcgggcgacg tcgtcctggt gccggcgccg ggcagcaccg
     2701 tggcccagtg gccgggcggc ctccgcagca ccaccgaccc tcggacgacg cacggggcgc
     2761 cgtgggacta catcgcccgg gtgccgatca tcctccgcgg ccccggccac gtgccggcgg
     2821 gcgccgtgcc ggatcggccg gtggacgtgg ccgacatcgc tcccacgatc gcggacctgc
     2881 tcgcgttcga ctgggaggcg cccgacgccc gctccctgga ggaggccctg ctcccggacc
     2941 gcgacccgcc cagggtgatc acgctcgtcg tgcacgacgg cggcgggtgg aacgtgctgg
     3001 agcggtggcc ggaggcctgg cccttccagc gcgggctcgc cgccgagggc acgaccttcg
     3061 ggaacgcgac ggtgggctcc gcgccgccga tcacggcgcc gatccacgcg aacatgggga
     3121 cggggaccta tccgcggacc cacgggatcg ccgagaacac gggccgcctc cccgacggct
     3181 cgctcggaga gctcttcttc cacgaggccg acccacgcct catggaggcc gagaccgtgg
     3241 ccgacgcctg ggacgtcgcg caggggaacg aggcgtgggt cggcctcgtg ggctacgagg
     3301 cgtggcacct ggggatgatg agccgaggca cgcgccaccc gggcggggac cgggacgtcg
     3361 ccgtgctctg ggaccatcgc gagaccgacg acctcttcac gaacgaggag gtctaccgga
     3421 tgcccgagta cctccggggc cgggaagcgc tggaggagcg cgtccacgag ctcgacctcg
     3481 cggacggagc ggccgaccgc cggtggatgc aggccgacct cgacgacatc ttcttcatcc
     3541 ccgccacccc cgccttcgtc gagtaccaga ccgacctcgt cctggagatg atcgagcgag
     3601 aggggatcgg gaccgacgac gtgaccgacc tcctgttcgt ggagctgaag ccgagcgaca
     3661 acgcaggcca cctgtggaac atggagtccc cgatgttcga ggaggtgctg cgggcgcagg
     3721 accgcgcggt ggagcgcatc gtgggggcgc tcgacgagcg ggtcggtccg gatcggcacg
     3781 tcgtgatcgt gagcgccgac cacgggcagt cgccccgtcc cgacgtcgta gagggcctgc
     3841 ggatcgaccg cttccgggtg caggaggcgc tcaacgacgc tttcggagcc gaggtggtgg
     3901 aggcggtgca ccccgacgac ctgtacctcc gctgggacgt gctcggtgag atcggggcca
     3961 cggccgagga cgtggctcgg gcggcggcgt cgctccgctt cgaccaggtg gcgcctcaag
     4021 gcaccgacct ggaggcgctt tcggaggagc tggcggccgc ccgacccttc gccgccgcga
     4081 tcccggtccg gcggctcgtc ggactgagcg aggaggaagt cgccgcgctg ggagcgggcg
     4141 ccttcccaga ggccgacctg acctcgccgc ccgacctggc ccggctcctc agacgttgag
     4201 gtagggctgg gccgggcggg ggacggcctt caccgaggcg gcgtccacga cgacctgatc
     4261 cggcagcgga tagagcgttc cctggacccg cacccactcg tcctcctcga aggccccggg
     4321 gggagcgccc accacccgga cctgcacgct gagcgcgtcg gccacgcagc acgagacgag
     4381 gaaccgggtc agcacgaact cgtcagcggg cgtcccctct cgacgcacga cgaagcccac
     4441 gaacgagacc tcgctgccgg cgcgctcggt cagggagtcg gcggccgtct gggaccacat
     4501 cgcgccggcc acgtccacga gcgtcacttc acccgacgag ggctcgccgg ccgcggtgac
     4561 gaaacccgcg ctcaccgaac ggcgggaggc ggcgtaggat ccgagcgccg tgggcggaag
     4621 ggccagcacc gccacgaccg gcacgagcac gagcgcgagc ccccacgtgt cgcggaccgc
     4681 cagcggctcc gcgcgctcgg tcctcgcggt gagaagacgc cccagggcgg tggcggtgag
     4741 gaggaccgct cccacgggca cgacccagaa gatgcgagac gagaggtaga gggtccagcg
     4801 gtccgtgacg aggaggaacc agaagacccc cgcccacgcg gccagcgcga ggccgaccgt
     4861 gacccgggtc gggctcagcc gcctcatccg gccagcacct ccacccagag ggcgccggcc
     4921 agcgtcacgg cgcccaccac cgccacgacg gtccgcagga acccgcggct gaacgtcccc
     4981 acgtagagga acccgagctt cgcgtcgacc atcggcccga acaccaggaa ggccagctgc
     5041 gctcccgccc cgaactgcac gaacgacgcg gcgacgaagg cgtccgactc ggagcagagc
     5101 gagaggatcc cggccagggt catcatggcc aggatgttca ggacgggggt ctcggccacc
     5161 gccccgacga tcccctgggg cacgaacgtc tggagggccg aagccacccc ggccccgatc
     5221 acgaggtagc gaccgagcat gacgaagtcg gaggagagat gggcgaagta ggccggccat
     5281 cgccgtgcga gcggctcccc gggctcgtgg tggtcctcgg gggcgcgcag cagctcccgc
     5341 ttccccttcc gggctacgac ccatccgacc gcgagggcca cgacgaggcc caagatcgcc
     5401 cggcccccga ccatcaccca caacacgtca cgacctcggt aggcgacggc cgtggctacg
     5461 agcacgacgg gattgaggat cggcgcggcc agcatgaagg tgatggcggc cgccggcgtc
     5521 aggcccttga ccacgagccg ccgggccacg gggaccgacc cgcactcgca gacggggaag
     5581 acgaagcccc ccaggccggc caccggcatc tggaccgggc ccggcaggcg cgccaggcgc
     5641 gcgaacagcc gtgccggcac gaagacctcg atcgcggcgg agacgatcgc tcccagcagg
     5701 atgaagggga cggcctccac gaggagcgag gcccagacga ggaggaagtt cctgaccgga
     5761 gggaggccag cggcgtcgag gagccgcagg atcgtcccgg acgcgatgag gacgaggagg
     5821 agcacgaggc ccccgcgcca cgcgggacgt cgctcgccgg ggaggcggag gcgaggcttc
     5881 tcgatcacgg ccatcagcag tacctcccga actcgacggc gcggcccccg aagacgtcgg
     5941 cgaacatcgg gccgccgatc gacgcccgga tcccacgcag ggcccggatc acatcctcct
     6001 ggctggcgtc ggcttccacg agcacgtcct gatcgaggaa gagcccgccc accgccacgg
     6061 cctcggtcaa gccggcgccg gcgccctcca cctcagcccg gattcggtcg gcgcccaccg
     6121 cctcctcggc cgccagcggt ccggtggagg tgaccacgac cgcggtcgag ggagggacgg
     6181 cctgaacgac cctctgcaga gcccggtcca tcgccggcac gcggtcgtgc atcaccacgg
     6241 cgagcaggtc gggcacgtca tcggcgccgt atcccccggc caaaaggtcc gcagccgcgt
     6301 cggcctggcg gaccgcccga tccttcagca cgacctcgtc ctggtcggtt cgcagatgcc
     6361 aatccttgcc gatcacgccc cggtccagag cgctcgcgcc gaccaggccc acgagtggac
     6421 gctggcccag aacctcgtcg aggtcgtccc cgagggccgc gatcaccgag tacggggcgc
     6481 cccgtcccca ggcggggacg gccttgccct cctcgttccg tagggtgctg ccggtgatgc
     6541 cgtgctcgcg ggggagtcct ccggtgccga tcgtggtcag gaccgccgta gggtcgaggg
     6601 ggagcgatcc gacccgcgcg tccagcgtgc ccgcggcctc ccggaggagg cgccggagcg
     6661 tgggccacgc ggccgggctc gcctcgaggt cgtcggagcc gacgcccttc cagaccacga
     6721 ggacgaccag gcgggcggga ccgccggcgg ccactcccgg gatcgcctcc cccgagcgga
     6781 cccccgggtg ggggacccgg acgtcgagga tggcggccgc ggtcggggcc aggtcgacga
     6841 gaggcgtggc ggcggggacc tcggcggcgg ggtcgatccc cgccccctgg aagacgagag
     6901 gcactcggcc ggcatccacg tcctccacgg agggccacgg cgcggagaga tcctcgagcc
     6961 cggtggcgcc ggccacggtg gcgtcctcgc tcgtgaccac gaggacctcc ggggagcggc
     7021 cctgccggta gccgtcggcc agcaggcgac ggagctgggg gtcgagggag cagaacggcg
     7081 cccggacctc cggggcggcc tgccggcggc aggactctcc tacgcagaag gcgcggagga
     7141 gaagagccgg cgccgcgagg agacccgcgg ccgcgacgat gaccagggtg cgacggccca
     7201 ggccgctcgc gaaggacggc ggggggatgg aggtcatgtg cctcgggcca cgatacacga
     7261 gcctccctgc gggatactcc ggatgatgat gcgggcgctc gtcctcgtcg tggcggtgct
     7321 ctcggcgtcg tgctcggggc cgccggaccc tccgccggga gcgcggagcg tcgaggagat
     7381 ggcgcgcgag gtgggggccg acgtgctgga ggcgatgcgg cgggggtacg tccccggccg
     7441 ctccggcgag ctggcgctga tccccaggcc ccaccacgtg atcgtgcggc ggggaagctg
     7501 ggacccgggc ggtgagccga gccccgacac cagccactcg accccgtggg cctaccacca
     7561 gcgtgtgccg atcctcctct acggaccggg gttcgtccgc gaggggaccg tctcggagcg
     7621 agcggtcgat ctggcggacc tggccccgac gctcgccgag ctcatgggct tcccgttccg
     7681 ggctcgggac ggtgtcccgc tgtccgaggc gctggcgccg gggcagcggg gggcgccgcg
     7741 ggccatcgcg ctcgtggcct acgacggcgg cgggtggaac ctcctccagg cctaccccgg
     7801 cgactggccc ttcctgcgag cgctgatggc ccgcggcgcc ctgttcacga acgccacggt
     7861 gggctcctcg ccctcggtca ccgcggcggt gcaccccacg atgggcaccg gtgcctaccc
     7921 tgccgcccac ggggtcccgg acaactcggc acgcctcccg gacggcacgg ttggggacgc
     7981 gtacctcggt cgaaccgatc ccaggctgct gcgcgtcgag acgctggccg acgcgtggca
     8041 cgaggcgacc ggcgcgtgga cggggatcgt ggccacggag tcctggcacc tggggatgct
     8101 cgggcgcggc gccgggcccg gcgccgcgcc cgagcaggtg gccgtcctct ggaaccggcg
     8161 ggagcggcgc ttcttcacga accccgagcg ataccgcctc ccgggatacc tgcccgccga
     8221 ggactcgctc gaggcccggc tccgggagct cgacgggagc gacgggtcgc tcgacggccg
     8281 gtggatgggg aacgatctcg aggaccccgg cgtgctcccc ggaacccccg ccttcgtccg
     8341 ttatcagggt gacgcggtcc tccggatcct gggtcgagag cggatcgggc gcgacgatat
     8401 caccgacctc ctcttcgtcg agctcaagtc caccgaccgg ggcgcgcatc tctggaacat
     8461 ggtcggtgag gagcagcccc acgtccttcg ggctcaggac gacctcctgc gggacgtggc
     8521 agccgccctc gacgccgaag tgggggaggg gcgttgggtc ctggcggtca ccgccgacca
     8581 cgggctcact cccctgcccg aggaggtggg cggggtccgc ctccaccccg acgcgctcgc
     8641 tgccgccgtt gaggagcgcc tcggcccgat cgtcgaggcg gccacgccct cctcgctctt
     8701 cctggacatg gacgaggtcc gctcgcgcga cctcgatctc gacctcgtgg ccacgatcgt
     8761 cggcgatctc cgttaccgcg acgggatccc cgagggtgtg gacctcgagc ggatccccgg
     8821 ggacctcctc gaccgacggg tgttcgccgc tgtcctgccg gggccttacc tggaaggcct
     8881 caccgcggag gagaccgccc gcgccggcct cgggatctac gaggagggcg acctcacggg
     8941 ggtggacgcg gcggcgaggt tcgcgaggct ggcgggctga cctcctcggc ctcgatcttc
     9001 cgctccggcc ggccgccaag cgcccgcgac cacagcccga tcagcagggc gatcagcagg
     9061 aaggccgccc ccgagccgat cagcaccatc cgagccgtgt cgggcgtgag gaccagccag
     9121 agcaggaggg cggggagcac ccaggagagg ttgtaggaga cgtcctggag gctgaacccc
     9181 cgaccgcggt acgagtcggc cagcgcctgc tgcatcaggg tgtccacggc caccttgccc
     9241 aggaagaagg ccacgccgac gcagaaggcc agcgccgaga tcccgagcat gttgattacg
     9301 cccccgaacg ccagcatccc ggccgcgccg aacagcagcg cgcccgacac gatcctggcc
     9361 ggccgcacgc ggtccttcag ggcgtgggcg atcacgaacc ccacgacggc gcccagcgcg
     9421 ccggcggccg ccgggatcgc ggtcgtgagg gtgtcctgcg aggcgatcag gtctcgagag
     9481 atgaacaccg tggccagcac caccagcgtc aggagcgtgc ggacggagag gaacgagacg
     9541 atggccagcc ctgccatcgg ccgccgcccg atctcgcgga acccgtccag gaggtcacgg
     9601 aacacccgcc cgatctcctt ccggaggggc acgaccgcgc gggcctcgcc cagccggtgg
     9661 acgagcgacg ccgacgccgt cgccgccccg tacacgaggg cgcccgccag gatgatcagc
     9721 cgggcgtcca cgaagcccgc ggcgacgagc gccacccccg cgcccccgag ctggaagatc
     9781 gcgctcccgg cctgcgaggt ggcgttgccc tgcatcagct cgtcctcgcc gcccagcgcg
     9841 gcgggcaggc cggctccctt gatcgccagc accagccggg tccctcccag caccagcagg
     9901 aaggagccgt acagcgcgac gttcccgatc gcgtcgggcc ccaggatccc cacggtcagc
     9961 agcagcacgg cccggacgcc gttggccagg atcagcagca tccggcggtt ccagcgatcg
    10021 atgaagaccc ccatgaacgg gctgatgatc atgtagggga ggaaggtaag gaggacgatg
    10081 aggagggcct cccgcggcgt ggcggctccc tcgacgtcga ggtcgaagcc ggcgctccct
    10141 ccgaaggtga tcttcttggc cagggcaagc ccgaccaggc cgtcggcggc ctgcgccagc
    10201 cactgggcgc ccatcaggaa ggtgaagtct cgcttccgga acagggcgag ggagtctcgc
    10261 gcgatgctca cgggccgtga acctagcaca tccccggtcg tgctccgatg tagggtttcc
    10321 cgatgcgtcg cgtcactcgg tcggtcgcag cactcggcgt cgccgtactc ttcgccgcgt
    10381 cctgcacgga cgcagggccc gaaggcctcc gggccccccc cacggccgtc ccgacccccg
    10441 cgccgcccac cgcccttccg gcggaggcct ccccggcctg ctccctcccg cccgaacacc
    10501 tgctgcgcat ccgacgcggc taccatcccg agcgctcggg cgagatccag atcgtccctc
    10561 ggctcccggc ggtcgtcgcg tcgtggctct cccacgcggg gccctgggat cacgtccagc
    10621 gcgtgccgct cttcctctac ggcccggggc acgtgccggc cgtgggccgc gtggaccgtc
    10681 ctgtgacgct agcggatctc gccccgacga tggcgcggat cctgggctcc gacttcgagg
    10741 ctccggacgg gcggcccctg cgcgaggcgg tggccggttc ggatccacct cgcctgatcc
    10801 tcaccgtgat ctgggactcg gcgggacgca acgtcctcga cgagcacccc gaggcctggc
    10861 ccacgctgcg cgccctgatc ccacagggga cgtggttcga ggaggcgacc gtcgggacgt
    10921 cgccctcgac cacgcccgcc gtgcacgcca cgatcgggac cggcgtcttt ccgtcccggc
    10981 acgggctcgt cgacctccac ttccgctggg gcggcaggat ggcccacatc ctcgacgagc
    11041 gtcccgcctt cctctcgatg cccacgctcg ccgatcggta cgcggcgaac accggccagg
    11101 cggcgaaggt cgggctgatc gcctcgctgg ggacgctggg gctcatcggc cacggggcct
    11161 cgatcgaggg cggtggccgg cacctcgtgg cgatgcacca cgagggcggg tgggggatcg
    11221 gggaagcgta cgccgaggac ttccgcttcc ccgcgtacct ccgcgacgta ggcggcttcg
    11281 cggcgtcgct acggcgtctc gatctcgagg acggcgccgc cgacggtcgc tggctgggtg
    11341 agcccgtcct gaggccgggc gaggaccgct ccatctcgcc cgcctggacg gagcaccaga
    11401 cccgcgtcct cgaggaggtc gtcgagcgcg agggcttcgg ccgcgacgcg gtgccggacc
    11461 tcctcttcac gaactacaag cagatcgact cggtggggca ccggtggggg atgcacgggc
    11521 cccagatgga ggccgtggtc cgctcctccg acaccgcgct gaggaccctc atcgccatcc
    11581 tcgaccgcga ggtgggggag ggtgagtggg tgctggcgct caccgccgac cacggctcga
    11641 tccccgaccc cgccggggcg ccgatcatcg atctccggga gctgcggcgc gacctggcgg
    11701 aggccttcgg ggacgccgtg gagcaggtgc ggcccaccca ggtctgggtc gacgagcgtg
    11761 cgctcgcctc ccgaggggcc acgttggagg atgtggcgga gctgctcgga ggctatcggc
    11821 gctccgacaa ccacgagctg cccccaggag agcccgacac gcggctgttc gccgcggcct
    11881 tcccctcgga agtcctcctg accgcgtcct gcgtccccag ccaacgaggg gccttcgggt
    11941 agactgtcgc gccgaacggt cccaggatca tccgtgctga catcacgact gctgagcatc
    12001 gcgctcgtcc tggccgctgc ctgctccgga ggcgcggcca ggacgccgcc tgcggacccg
    12061 tcgccgacgg gacgatcagg aggcgcgccc gaggcgcggg agacaccgcg tcctccccgc
    12121 ggccctttcg agcaggtcgc ttgccgactg ccggagccgc acctccgccg aatctgggcg
    12181 gggtaccatc ccgggcgctc cggccacctt cagatcgtcc cgaagcctcc cgacgtcgtc
    12241 ggggcgtggc tctctcactc cggtccctgg aggttcgtcc agcacgtgcc gctcctcttc
    12301 tacgggccgg ggcacgtccc cgagggggtt cgggtgagcc ggacggcgac gctcgccgac
    12361 gtggcgccga cgcaggccgc gctcctcggc ttcgacttcg acgcgccgga cggagagccg
    12421 ctcgccgagg cggtggccgg cgccgagcgc cccccgaagc tcatcgtgac gatgatctgg
    12481 gactcggccg gcatgaacgt gctggaggag catcccgacg cctggcccac cctgggccgc
    12541 ctgatcgagc ggggaacgtt cttcgaggag acgttcgtcg ggtcgtcacc ctcggtgacg
    12601 ccggccgtcc acgccacgat cggcacgggg gctttccccc ggaaccacgg gatcgtggat
    12661 ctgcgcttcc gcgtggacgg ggtgcccacg gccgcccgag gcaaggccgc cgcgaacctc
    12721 ctggtcccct ccctctccga cctcttcgac gtcgcccgcg acaacgagcc ggtggtgggc
    12781 ctgatcggtt cggaggggac cctgggcatg ctgggccacg gcgctttctg ggagagcgga
    12841 gactcggacg tggccgtgac tcgcacccgg accgagtgga ccctggaggg gctgaacgcc
    12901 gaggccttcg ccttcccgga gtatgccgga ggcttcccgg gactcgagga ggcgatccgg
    12961 aggctggacc tcgaggacgg acaggccgac gggcgctggc tgggggagcc gatggacgag
    13021 ggcgtggagg gggtctcggc tacgccggcg ttcggcgagt accagcggtc gatggtccgc
    13081 gaggtgatcg agcgcgaggg cttcggccgg gacgaggttc ccgaccttct ctacgtcaac
    13141 ttccgcgaga tcgacgcggt ggggcaccgg tgggggatgc acggacccca gatggaggcc
    13201 gtggtccgct ccaccgacga ggcgctggcc gacctcgtgc ggaccctcga tcgagaggtg
    13261 ggggcggggg agtgggtcct cacgatgacc gcggaccacg gggcgacccc cgaccccgag
    13321 ccgctgggag ggttcgtcat cgggccccag gaggtcaaga aggacatcga ggcggagttc
    13381 ggccccatcg tcggggacct gcgcgtcact cagatctggc tggagctcga ggagatggcc
    13441 gagcgggggg tcagcctgca ggaggtggcc tcctttgtgg cagactaccg gaggtccgag
    13501 aacgcctccg atcccgcgtc gctgaccgcg gagcaggggg aggagcgcgt gttcgggacg
    13561 gtcttcccca gcgcggtcct ggatgatcct ccgtgcatca tcacgacgcg gagtgggtga
    13621 cgaaggagtg cgatgaagac ggaacgggcc cgcgaggacg ccaccgcctc ggagcgctgg
    13681 gggccgctgg gggggccgat agcgtgggcc ggtcgggtgg ccgcccaccc cgggacggcg
    13741 ctggccgcgg tggccgcgat cgccctgctg atggcctggc agttcatcgt ggacgcgtcc
    13801 cgcgcggtcc ccgcgctcga cacggcctgg taccagtggc gggccgagta catccaggcg
    13861 aacgaccccg gatcgttggt cggcatccag ggggccgacg ggtccctggc cggcggctac
    13921 cgggtcgccg agcccgtgct ggccgcgctc cttcggatcg tgggaggcgt gggggcggag
    13981 gtgcccaccg tggtcctgtc ggtgttcttc cgagccgccg ccgcggtcgc cctcgccgcc
    14041 ttcgcctggc ggcaccggcg cagccggctc ctcttctacc tgacgctcgc cgtggtgccc
    14101 gccctcttcc tcctccaggg gttcttcggg tacctggaca acttcttcgc actggcgctc
    14161 acgggggcgg ccctcctcct cctggagccg atgcggacga gctgggtggc tcggctcgtg
    14221 gtcatcgcga tgctcttcct catgggcctc acgcacccca cgacgctcgc gctcttcctg
    14281 ctggcgctgg gcgcggttgc cggctatcag ttcctgcggg aaggcctctc gatccgctcg
    14341 gccctccgct ccgacgtgat gccgatgctc tgggccgggg tggtctccgt ggtgctgatg
    14401 ttcgcggcct ggctcggcgg gatgtggggc cccagcgctg gcctcaacga ggccgcggtg
    14461 cccccgcccc agccggtctc gtacttcgtg gaccgctcga tcaacgtgtt ccgaggcatg
    14521 cagccgatca cgctcgtgcc gctcttcctg atcgggttcg tggcgctcgc cgtggacgcc
    14581 tggaggaggc gcgagcggct cgcggagatc acagtggcct gggacctccc gctgctcggg
    14641 atgctggggt tcctcctcgg ggcggcctac ccctacttcc gcttcttcaa cgcgaccctg
    14701 gcgccgctgc tgaccatcgc cgtggggttc gcggtcatca tcggcgcggc ctcgcggctg
    14761 cggagagagg ggacggcgcg tctcgcctcg gtggtggccg tcgccgtgac cgtcggggtt
    14821 ctggcgagct ggtggatcgg gggcctctcg tcgtggaaca gcaagagcac gtggctgacc
    14881 tccgaggttc ggacgagcct gggcgcgatc tcgggctacc tcgagggcga gccggcggga
    14941 cgcaaggcgg tcgtcgtggt ggacgctcag ccgaacggtg tcgtgccgta cagcaggtac
    15001 aaggagttcg ccaacgcggt gtacgccggg atcccggggg accgcatcga cgacacggag
    15061 ctcttcttcg ggcggatcga ggacctggag gcggggacga ccacgtcggt cggggacgag
    15121 cagtacgaca ccatggcccg ggacagcgcg gctgaggccc tgccgctcct ggagcaggag
    15181 gcggtggtgc ttatgccgct cgtcttcaac gagccctcgg acaacgccgc gttcctcgag
    15241 gggtgcgccg agtgccggga gctgggggac agcgaggtct acgcgatccc cgagctctcg
    15301 acggcggcgc tctcggagga ggccgtggcc gcgggggccg aggcggccga ccaggcgagg
    15361 gcgttcgccg agagcccgcc cgggcccttc gagggcctcg ggtcgagcct gctgtcgctg
    15421 ctggggctgc tgctcctcct ggccgtcccc gggtggctgc tgctgcgcgg gatcccgggc
    15481 cgggacccga tcgaggcgat cgcgctcgtc ccgatgctgt cggtgacgct ggtcacgacg
    15541 gtcggcgtcg tggcgctggg gatcatccgg gggccgagga cctcgctcgt ggggtggatc
    15601 gtgtgggccg tctcggtggg ggtcgcggcg tgggccgcgt tcggc
//
LOCUS       DASUTZ010000023         3056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_394420, whole genome shotgun sequence.
ACCESSION   DASUTZ010000023 DASUTZ010000000
VERSION     DASUTZ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394420"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1524)
                     /locus_tag="VF097_01100"
     CDS             complement(<1..1524)
                     /locus_tag="VF097_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX6261422.1"
                     /translation="MIDVQGVRKAFGDRVLFRDASVRIAARDRLALVGPNGSGKTTLV
                     EMIVGLQEPDAGDVLIPKGTVIGYLPQETDALRGRTVLEEVLSAGRAMTEAGHRLEVL
                     QRELEETGPDEALLAEYARLQERFETLGGYSLEAEAASICAGLGFREEDLRRRTGELS
                     GGWMMRVALAKLLLASPDCLLLDEPTNHLDLESVVWLEGFLRSYEGAVVLISHDRELM
                     NGLATRVVEIENGTLVSYAGNFDAFEAQRALRLEHAEAAARNQQRKIAQSERFIERFR
                     AKNTLASRVQSKIKQLEKLERVEVPRERRRTMGLWFPTPPRPGRVVVELKDVGFSYGE
                     LEVYRGLSLAVERGRRLALVGPNGAGKSTLLKLIAGALEPTSGERRLGANALVGYFAQ
                     HQIEALEPRNTVLAELERAVPRGVEGKPRDLLGRFLFSGDDVEKRVSVLSGGERTRLA
                     LARLLVQPFNLLCLDEPTNHLDIPSRDALEGALAAYEGALVLITHDRHLIRSVADTIV
                     "
     gene            1580..2029
                     /locus_tag="VF097_01105"
     CDS             1580..2029
                     /locus_tag="VF097_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017302349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEX6261423.1"
                     /translation="MRLRSPRDWGAHHHPANIRHLGPKRTVGERVADGVAAAVGSWPF
                     ILIQSSLLATWVLANALLIGDWLGGRPFDPYPFILLNLVLSFQAAYTGPIVLMSQNRQ
                     TAKDRDEAEHDYEVNRQTLRMLGGLEERLADIEESLRALRPPGRSPT"
     gene            2226..>3056
                     /locus_tag="VF097_01110"
     CDS             2226..>3056
                     /locus_tag="VF097_01110"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX6261424.1"
                     /translation="MAGYANPEALVETDWLAERLGDPDVRIIEVDEDTSVYRTGHIPN
                     AVAWDWFEDLHTDVGRDYVDQDGLARLLSEAGVGPDTTVVLYGGSDNWFAAYAYWLLK
                     YLGFEGAKLLNGGRKKWEAEDRELTSEVPSFQATDLRIDGPVRKDLRAFRDEVLHRLG
                     SASFVDVRSPEEYRGEKLAPDHLPQEQSQVPGHIPGAANVPWAQAVNDDGTFKSAEEL
                     RELYASKGATGDTDVIAYCRIGERSSHTWFALHELLGIENVKNYDGSWTEYGSLVGVP
                     V"
BASE COUNT          479 a         1076 c         1054 g          447 t
ORIGIN      
        1 gacgatcgtg tcggccacgc tccggatcag gtgccggtcg tgcgtgatca ggacgagcgc
       61 gccctcgtac gcggcgagcg cgccctccag cgcgtcccgg ctggggatgt cgaggtggtt
      121 cgtcggctcg tccaggcaca gcaggttgaa cggctggacg aggagccggg ccagggccag
      181 ccgcgtccgc tcgccgccgg aaagcaccga gacgcgcttc tccacgtcgt cgccggagaa
      241 gagaaagcgg ccgagcaggt ccctgggctt gccctccacg ccccggggca ccgctcgctc
      301 cagctccgcg agcaccgtgt tgcgcggctc gagcgcctcg atctggtgct gggcgaagta
      361 gcccacgagc gcgttcgcgc ccagcctccg ctcccccgac gtcggctcca gcgcgccagc
      421 gatcagcttc agcagggtcg acttccccgc gccgttcggg ccgacgagcg ccaggcggcg
      481 cccccgctcc acggccaggc tcaggccccg gtacacctcg agctcgccgt aggagaagcc
      541 cacgtccttc agctcgacga cgacgcgccc cggacggggc ggcgtcggga accacaagcc
      601 catcgtcctc cgccgctcgc gggggacctc gacccgctcc agcttctcga gctgcttgat
      661 cttgctctgc accctgctgg ccagcgtgtt cttcgctcgg aaccgctcga tgaagcgctc
      721 gctctgggcg atcttccgct gctggttgcg agccgcggct tcggcgtgct ccaggcgcag
      781 ggcccgctgc gcctcgaagg cgtcgaagtt ccccgcgtac gagacgaggg tcccattctc
      841 gatctccacc acccgcgtgg ccagcccgtt catcagctcg cggtcgtgcg agatcagcac
      901 caccgccccc tcgtaggagc ggaggaaccc ctccagccag accaccgact ccaggtcgag
      961 atggttcgtg ggctcgtcga ggaggaggca gtcgggtgag gccagcagga gcttggccag
     1021 cgccacgcgc atcatccagc ccccggagag ctccccggtc cggcgtctga gatcctcctc
     1081 gcggaacccg aggcccgcgc agatgctcgc ggcctcggcc tccagcgagt acccgcccag
     1141 cgtctcgaag cgctcctgga ggcgcgcgta ctccgccagc agcgcctcgt cgggcccggt
     1201 ctcctcgagc tcccgctgga ggacctcgag acggtggccg gcctcggtca tcgcccggcc
     1261 ggccgacagc acctcctcca acacggtccg gccccgcagc gcgtcggtct cctgcggcag
     1321 gtagccgatg acggtgccct tcgggatcag gacgtcgccg gcgtccggct cctggagccc
     1381 gacgatcatc tccacgagcg tggtcttgcc cgacccgttg ggccccacga gggccagccg
     1441 gtcgcgcgca gcgatgcgga cggaggcgtc ccggaacagc acccgatcgc cgaacgcctt
     1501 gcggaccccc tggacatcga tcatctgccg agggtacacg cccgcgcgat gatgacctcc
     1561 tcgacgaagg gagacagcta tgcgactcag gagcccacgg gactgggggg cccaccacca
     1621 ccctgcgaac atccgccatc tcgggccgaa gcgcacggta ggagagcgcg tcgccgacgg
     1681 tgttgccgcg gcggtcggct cctggccctt catcctgatc cagtcgtctc tgctcgcgac
     1741 gtgggtcctg gcgaacgcgc tcctgatcgg ggactggctg ggcgggcggc cattcgaccc
     1801 ctacccgttc atcctgctca acctcgtgct gtcgttccag gccgcgtaca ccggtccgat
     1861 cgtgctgatg agccagaatc ggcaaaccgc caaagatcgc gacgaggcgg agcacgacta
     1921 cgaggtgaac cgacagacgc tccggatgct cggcgggctg gaggagcgac tggccgacat
     1981 cgaggagtcg ctccgggctc tcaggccgcc agggcgatcc cccacttgac gaatcggagt
     2041 cgttcgaggt agaaagccga aaccctaccg atgggatagg gaatgatccg cacgcgctgc
     2101 tgcatctgtc gccggttcgc ccccatgggg gcggaggctc gcgtgcggcc gtagcgcgaa
     2161 gcgaccatcg ttcgttccgg gagccgccgc gaggcggctc tttcgtttgg aaggagggag
     2221 ggaacatggc gggatacgcc aatccggagg cgctggtcga gaccgactgg ctcgcggagc
     2281 gcctgggcga tccggacgtc cggatcatcg aggtcgacga ggacacctcc gtgtaccgta
     2341 ccggccacat cccgaacgcg gtggcctggg actggttcga ggacctccac acggacgtgg
     2401 gcagggacta cgtggaccag gacggtctcg cgcgcctctt gagcgaggcg ggggtggggc
     2461 ccgacaccac cgtggtccta tacggaggca gcgacaactg gttcgcagcc tacgcgtact
     2521 ggcttctgaa gtacctgggc ttcgagggcg ccaagctgct gaacggcggg cggaagaagt
     2581 gggaggcgga ggatcgagag ctcacgagcg aggtgccctc gttccaggcg accgatctcc
     2641 ggatcgacgg gccggtacgc aaggacctgc gggccttccg cgacgaggtg ctgcaccggc
     2701 tggggagcgc ctcgttcgtc gacgtccgct cgcccgagga gtatcggggc gagaagctgg
     2761 cccccgacca tctgccgcag gagcagtccc aggtgccggg gcacatccca ggggccgcga
     2821 acgtcccctg ggcccaggcg gtcaacgacg acgggacgtt caagtccgcg gaggagctgc
     2881 gcgagctgta cgcctcgaag ggcgcgacgg gcgacacgga cgtgatcgcc tactgccgga
     2941 tcggggagcg ttcgagccac acgtggttcg ccctccacga gctgctgggg atcgagaacg
     3001 tcaagaacta cgacgggtcc tggaccgagt acgggtccct cgtcggggtg ccggtc
//
LOCUS       DASUTZ010000024         4966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_783584, whole genome shotgun sequence.
ACCESSION   DASUTZ010000024 DASUTZ010000000
VERSION     DASUTZ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783584"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /gene="secY"
                     /locus_tag="VF097_01115"
     CDS             <1..1089
                     /gene="secY"
                     /locus_tag="VF097_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX6261425.1"
                     /translation="ALQNFAIFALGIMPYITSSIIMQLLTVVIPKLEQWQKQGEVGVK
                     KITQWTRYMTVTLAVLQSTGLLFLINNQSIEAGTPVFQEFTPPRVAFMVLILTAGTAL
                     IMWLGELITQRGIGNGMSILIFVSVVSALPSQGTLLWEQLSRPMFAVMILLGIAIIAA
                     VVFMEQGQRRIPVQYAKRVVGRRMTSGGSTYIPLKVNQSGVIPIIFASSVLYFPTLLA
                     SVIQWGPFQRFVNNVLVNQASPGYIITFGLMVIFFAYFYTAITFNPVDTADNIRKYGG
                     FIPGIRPGRPTADYLARVLNRITLPGSLFLATIALVPSIAFALLLVDPNAFPFGGTTI
                     LITVGVALETSKQLESQLTMRHYEGFLR"
     gene            1094..1747
                     /locus_tag="VF097_01120"
     CDS             1094..1747
                     /locus_tag="VF097_01120"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013016488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX6261426.1"
                     /translation="MRIVLLGPPGAGKGTQAHRLADYFDARLISTGDMFRRNVRHETP
                     LGLKAKSYMDAGELVPDDVVIDMLFSELDKTPNGWLLDGFPRTLPQAEALERGLEERG
                     KQLQAVLQFVIPDEATIARLAGRRVCRRCQRTYNVEVKPPRIEGVCDACGGELERRSD
                     DEPETVRHRLEVYARDTAPLIDFYRERGLLRPVDAVGEVEEVTRRAIEALADLPDPS"
     gene            1744..2490
                     /gene="map"
                     /locus_tag="VF097_01125"
     CDS             1744..2490
                     /gene="map"
                     /locus_tag="VF097_01125"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013921140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX6261427.1"
                     /translation="MIIRKSREELELMRRAGRITARARDAVVEAVRPGITTAELDSIA
                     EKVIRDEGATPSFKGYRGFPASICASINEEVVHGIPAERELREGDLFKVDVGAIWDGF
                     HGDSAVTVFVGGPPSETAEELVEATRRSLEAGIEWLRPGGRISDVGHAVQEVVEEAGF
                     NVVREYVGHGVGRALHEDPHIPNYGAPGHGPEIKPGLVVAIEPMVNVGTWETKVLPDD
                     WTVVTKDGELSSHFEHTVAVTEDGPEVLTR"
     gene            2617..4185
                     /locus_tag="VF097_01130"
     CDS             2617..4185
                     /locus_tag="VF097_01130"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HEX6261428.1"
                     /translation="MHRSSLSRSFLTRRICLPLTVIALAIAAAGIGATPAAAASIVVT
                     QTDDSESGPGCTLRQAIRAANANAPVLFCFHPGTSAPDTIVLPAGTYALSQAGVDDTA
                     EDGDLDIADDLTIVGAGAADTIIDATDMTNERAFQVLSGVTATISGVTITGGRDNGFS
                     GGGVLNQGTLTLDGVRVTENETSATGGGVSNDGTLTVRRSTIDNNRALAGAGLVHAGG
                     SATLVSTTISGNEAIGSGGGVLSSAPGTLTHVTITRNVADADGEDVIDGDGGGLVATN
                     ALNLARSIVADNTDGSPGAEAPDCVGGPTSQGHNVIGDTSGCSFVAHGTDRVDVDAMV
                     APVLKMNGGPTPTHRLLGGSPALDRVPAADASCGGTDQRGVPRPQRAGCDAGAYELAL
                     CMGRTVGIVGTPGADALVGTAGDDVVLALGGRDVIRTRGGRDTVCGGPGADTIRLGGG
                     PDRARGEGGGDLIVGGGQSDVLRGNGGSDRLRGGAGRDRLFGEGGNDDLHGGGGRDRC
                     RQGPGSGRVRSCEA"
     gene            4309..4422
                     /gene="rpmJ"
                     /locus_tag="VF097_01135"
     CDS             4309..4422
                     /gene="rpmJ"
                     /locus_tag="VF097_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEX6261429.1"
                     /translation="MKVRPSVKRMCEKCKMIRRKGRVMVICSNPRHKQRQG"
     gene            4426..4797
                     /gene="rpsM"
                     /locus_tag="VF097_01140"
     CDS             4426..4797
                     /gene="rpsM"
                     /locus_tag="VF097_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX6261430.1"
                     /translation="MARIAGVDLPREKRVEIALSYIYGIGRTRARQTVEGCRLDPGTR
                     VRDLAEDEVVRIREWIDREYKVEGDLRRDTAQDIKRKIEIGSYQGLRHRRSLPVRGQR
                     THTNARTRKGPKKTVGIKRKK"
     gene            4814..>4966
                     /gene="rpsK"
                     /locus_tag="VF097_01145"
     CDS             4814..>4966
                     /gene="rpsK"
                     /locus_tag="VF097_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX6261431.1"
                     /translation="MAKPKKKTRKKEKKNVTHGAAHIKSTFNNTIVTITDLEGNTLSW
                     ASAGNVG"
BASE COUNT          836 a         1654 c         1751 g          725 t
ORIGIN      
        1 gcgctccaga acttcgcgat cttcgcgctc gggatcatgc cctacatcac gagctcgatc
       61 atcatgcagc tcctcaccgt ggtgatcccg aagctcgagc agtggcagaa gcagggcgag
      121 gtcggggtga agaagatcac ccagtggacc cgctatatga cggtgacgct cgccgtcctg
      181 cagtccacgg ggctcctctt cctgatcaac aaccagtcga tcgaggccgg gacgcctgtc
      241 ttccaggagt tcacgccgcc ccgcgtggcc ttcatggtgc tgatcttgac ggccggaacg
      301 gccctcatca tgtggctggg cgagctcatc acccagcggg ggatcgggaa cgggatgtcg
      361 atcctgatct tcgtgagtgt tgtctctgcg ctgccctcgc agggcaccct gctctgggag
      421 cagctctcca ggccgatgtt cgccgtgatg atcctcctgg ggatcgcgat catcgcggcc
      481 gtcgtcttca tggagcaggg tcagcggcgc atccccgtcc agtacgcgaa gcgggtggtc
      541 gggcggcgga tgacctccgg cgggagcacc tacatcccgc tgaaggtcaa ccagtccggg
      601 gtcatcccga tcatcttcgc ctcgtcggtg ctctacttcc cgacgctgct ggcctccgtg
      661 atccagtggg ggcccttcca gcgattcgtg aacaacgtgt tggtcaacca ggccagcccc
      721 ggctacatca tcacgttcgg gctgatggtg atcttcttcg cctacttcta cacagcgatc
      781 acgttcaacc cggtggatac ggccgacaac atccggaagt atggggggtt catccccggc
      841 atccggccgg gccgccccac ggccgactac ctggccaggg tgctgaaccg catcaccctg
      901 ccaggctctc tcttcctggc gacgatcgcg ctggtcccct cgatcgcgtt cgccctcctc
      961 ctggtggacc cgaacgcgtt ccccttcggc ggcaccacga tcctcatcac cgtcggcgtg
     1021 gcactcgaga cctccaagca gctggagtcc cagctgacga tgcgccacta cgagggcttc
     1081 ctgaggtagc ggcgtgcgca tcgtcctgct ggggcccccg ggggccggga aggggacaca
     1141 ggcgcaccgc ctcgccgact acttcgacgc ccggctcatc tccaccggcg acatgttccg
     1201 gaggaacgtc cgccacgaga ctccactggg cctgaaggcc aagtcctaca tggacgcggg
     1261 cgagctcgtg ccagatgacg tcgtgatcga catgctcttc tcggagctgg acaagacgcc
     1321 gaacggctgg ctgctcgacg gcttcccccg gaccctgccc caggcagagg ctctggagcg
     1381 aggcctggaa gagcgcggca agcagctgca ggccgtgctc cagttcgtga tccccgacga
     1441 ggcgacgatc gcccggctcg ccggcaggcg cgtgtgccgc cgctgccagc gaacctacaa
     1501 cgtcgaggtg aagccacccc ggatcgaggg cgtctgcgac gcgtgcgggg gcgagctgga
     1561 gcggaggtcc gacgacgagc ccgagacggt ccgccaccgc ctcgaggtct acgcccgcga
     1621 cacggcgccc ctcatcgact tctaccggga gcgcggcctg ctccggccgg tggacgccgt
     1681 cggggaggtc gaggaggtca cccgccgagc gatcgaagcg ctcgccgacc tcccggaccc
     1741 ctcgtgatca tccgcaagtc ccgcgaagag ctcgagctca tgcgtcgggc cggacgcatc
     1801 accgcgaggg cccgagacgc ggtggtggag gccgtccggc cggggatcac gacggccgag
     1861 ctcgactcga tcgccgagaa ggtaatccgg gacgaggggg caaccccgtc gttcaaaggg
     1921 taccgggggt tcccggcctc gatctgcgcc tcgatcaacg aggaggtcgt gcacggcatc
     1981 cccgcggagc gcgagctccg cgagggagac cttttcaagg tggacgtggg ggcgatctgg
     2041 gacgggtttc acggcgattc cgcggtgacg gtcttcgtgg gtggcccccc gtccgagacg
     2101 gcggaggagc tggtcgaagc cactcgtcgc tcgctggagg cagggatcga gtggctccgg
     2161 ccgggcggga ggatctccga cgtggggcat gcggtgcagg aggtcgtcga ggaggctggc
     2221 ttcaacgtcg tccgcgagta cgtcgggcac ggggtgggtc gggcgctcca cgaggatccc
     2281 cacatcccga actacggggc tcccgggcac gggcccgaga tcaagccggg tctcgtcgtg
     2341 gcgatcgagc ccatggtcaa cgtcggcacg tgggagacga aggtcctgcc cgatgactgg
     2401 accgtcgtga cgaaggacgg cgagctctcc tctcacttcg agcacacggt ggccgtcacc
     2461 gaggacgggc ccgaggtcct cacgcggtaa cccccgaccc gtcgacggac ggctggaagg
     2521 ttgacgagcc aaaccgcgac tggttccatt acgcggtccg gtccgaacag tcggcgtctg
     2581 ctgggaccca tgggtcgccg cggaaggagt gaaacgatgc atcggtcatc cttatctcgg
     2641 tcgttcctga cccggcgcat ctgcctcccc ctgacggtga tcgcgctcgc catcgctgcg
     2701 gcagggatcg gcgccacccc ggccgccgcc gctagcatcg tggtcacaca gacggatgac
     2761 tcggagagcg gcccgggatg cacgctgcgc caggcgatcc gggccgcgaa cgcaaacgct
     2821 cccgtgctgt tctgcttcca cccgggcacg tccgcgcccg ataccatcgt gctcccggcg
     2881 gggacatacg ccctctcgca ggcgggggtc gacgacaccg ccgaggacgg cgatctcgac
     2941 atcgccgacg acctcacgat cgtcggcgcg ggcgcggccg acacgatcat cgatgcgacc
     3001 gatatgacga acgaacgggc cttccaggtc ctgtccgggg tcacggcgac gatcagcggc
     3061 gtcacgatca cgggaggacg tgacaacggt ttcagcggcg gaggcgtctt gaatcaggga
     3121 acgctgacgc tggatggggt acgggtaacc gagaacgaga cgtccgccac cgggggcggc
     3181 gtgagcaacg acggcaccct caccgttcgg aggagcacga tcgacaacaa ccgtgctctt
     3241 gccggtgcgg ggctcgtgca cgcgggcggg tcggccaccc tcgtgtccac cacgatctcc
     3301 gggaacgagg cgatcgggtc cggcgggggg gtgctctcgt cggcgccggg gacgctcacc
     3361 cacgtcacca tcacgcggaa cgtggcggac gcggacgggg aagacgttat cgacggcgat
     3421 ggcggcggcc tcgtggcgac caacgctctc aacctcgcgc gatcgatcgt ggcggacaac
     3481 acggacggct caccgggagc ggaggcgccc gactgcgtgg gcggccccac ctcccagggt
     3541 cacaacgtga ttggagacac ctcgggctgc tcgttcgtgg ctcacggaac cgaccgcgtg
     3601 gacgtcgacg ccatggtcgc tccggtgctc aagatgaacg gcgggcccac gccgacgcac
     3661 cggctgctcg gtggcagccc cgcgctcgac cgagtcccgg cagcggatgc ctcgtgcggg
     3721 ggcacggatc agcgcggcgt cccgagaccg cagcgcgccg gctgcgacgc gggggcgtac
     3781 gagctggccc tctgcatggg gcggaccgtt ggcatcgtgg ggacgccagg tgcggatgcg
     3841 ctggtgggga cggcggggga cgacgtcgtc ctggccctcg ggggacggga cgtgatccgg
     3901 acccgcggcg gccgcgacac ggtctgcggc ggtcccgggg ccgacacgat ccggctcggc
     3961 gggggcccgg accgtgcccg cggcgagggc ggcggggacc tcatcgtggg gggcggccag
     4021 tcggacgtcc tccgcgggaa cggtggctcc gatcgtctgc gtgggggcgc aggacgggat
     4081 cgtctgtttg gagaaggggg caacgacgac ctccacggag gtggcgggcg cgaccgctgc
     4141 cgccagggcc ccggctcagg acgagtccga tcctgcgagg cctgagggcc tcgtggacga
     4201 agggaccaca ctcgccctat acttgctgtt cggctccgct ggggcccatt ctggtctgac
     4261 aacccggacg aggcgcgcgt ggggacgcgc gcgccgggag cgtgaagcat gaaggtacgg
     4321 ccgtcggtca agaggatgtg cgagaagtgc aagatgatcc gccggaaggg gcgggtcatg
     4381 gtgatctgct cgaatcctcg tcacaagcag aggcagggct agcccatggc acggatcgca
     4441 ggagtcgatc tcccacggga gaagcgcgtc gagatcgcgc tctcctacat ctacggcatc
     4501 ggccggaccc gcgcgcggca gaccgtcgag ggatgccgcc tcgacccggg cacccgcgtc
     4561 cgagacctcg cggaggacga ggtcgtccgc atccgcgagt ggatcgaccg ggagtacaag
     4621 gtcgagggtg accttcgccg cgacacggcg caggacatca agcgaaagat cgagatcggg
     4681 tcctaccagg ggctgcggca ccgccggagc ctcccggtcc ggggccagcg cacccacacg
     4741 aacgcccgga cccgcaaggg cccgaagaag acggtcggca tcaagcggaa gaagtaacgg
     4801 gcgaaggggc gacatggcga agccgaagaa gaagacgcgc aagaaggaga agaagaacgt
     4861 cacccacggg gcggcccaca tcaagtcgac cttcaacaac acgatcgtca cgatcaccga
     4921 cctcgagggg aatacgctct cgtgggccag cgccggcaac gtgggg
//
LOCUS       DASUTZ010000025         9512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_783702, whole genome shotgun sequence.
ACCESSION   DASUTZ010000025 DASUTZ010000000
VERSION     DASUTZ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9512
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783702"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VF097_01150"
     CDS             <1..396
                     /locus_tag="VF097_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-binding domain-containing protein"
                     /protein_id="HEX6261432.1"
                     /translation="WWAVPDETIERAGVTPSALPGAAHAAEHAAIGLLPLVATCDRWD
                     IGGVSTAHHPDTGSCAIFIYDGYPGGAGIAERGFASGERLLRATLETIRACPCQSGCP
                     SCVQSPKCGNGNEPLDKDAAAALIEEMLA"
     gene            393..878
                     /locus_tag="VF097_01155"
     CDS             393..878
                     /locus_tag="VF097_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HEX6261433.1"
                     /translation="MIPIQIAVCGPGLATDEEERWAEEAGRRLAEEGAVLLCGGVSGV
                     MDAAARGAREAGGLALGILPGRDLESASEHLSAAVVTGMGEARNALVVRSADAVLAIA
                     GEYGTLSEIALALKMGVPVVGLHTWELAKGGRPVDAFVRADGVPEAVQEAMRLAWERR
                     T"
     gene            860..1687
                     /locus_tag="VF097_01160"
     CDS             860..1687
                     /locus_tag="VF097_01160"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX6261434.1"
                     /translation="MGATDVKRRVVNALAKHVANPVMRRLAGRIPGWALLETTGRRSS
                     RAHRIPVGDGLRGDAFWIVAEHGRHADWVRNVEADPRVRIRVRGRWRTGAAHILEDDD
                     PRERLRRLGGVNPWVIRVAGTEPLTVRVDLDPAPPRPGSILRDGLVAGLVAGAVAGAP
                     SGLHALRHGYLLDLLRAPGALVAPRAGAATQLSLTVPVHAAISAGWGIALATALPRRA
                     TALWGAAGGLVVAALDIVVIGSRIPVIRRLPLWPQVADHVAFGTVAGGILAGLRDPG"
     gene            complement(1684..3201)
                     /locus_tag="VF097_01165"
     CDS             complement(1684..3201)
                     /locus_tag="VF097_01165"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX6261435.1"
                     /translation="MLPLERFGDERTILAGTGQNVVGMVVAIVATFGTQVLITRALGA
                     SAFGIVTLATQSALVLGYFSRFGMDMAAVRRVAIDVGRGERSRVRAVVTRASIISMVS
                     SLIVAAAVFLLAEPIARSLAADAAGAATAAFRAAALAIPFTALVQVYLGATRGLKIMR
                     HTLYVFWMGQPLAWFALILAGWLVARSVGVTTLAYTVSWAGATAAAAWLWYRETRGFG
                     GDPPLSGEVRDLMRYGAPRAPAALFSQLLFWTDVFVLAVFASSREVGIYSAAARAGQV
                     LLLFIISVSLMFSPFVADLHARGEREKLDRLYKQLTRWTLAATLPVFIVLTAAPESAL
                     RLFGAEFEAGATALGILLLGQLVNVATGAVGFVLIMIGRTGWDLVVYAASVAFDIGAA
                     ALLIGGLGLGIEGAAIAGAATMALSKVARLWLVWRFLRMQPFTRHYALLAVPLTLGLG
                     AALGAHVLVAGASWPVDLAVTGGIGTLVYVTVLVAFGLPPAERRVLARLVGAVRG"
     gene            complement(3242..4825)
                     /locus_tag="VF097_01170"
     CDS             complement(3242..4825)
                     /locus_tag="VF097_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6261436.1"
                     /translation="MSRVLLIGWDGADWRILDPMLEAGLLPNLRSLIDRGARGVLKST
                     VPNHSWSAWPSFLTGVEPSNHGVYDIFVRNFEMRKQLPVTYGSIKERTMLPDLTAAGV
                     ETIMTNVPLTFPPQRIQGKLVSGGVLPKGRPYTHPEGLAAELEAAGKPFPINGMSWTT
                     YWNRPEPFLAEAMELTGQRIEANEHLLASTDWRFASLVYVATDRVQHCLAKYVSSDHP
                     DFSTLSKEPLAEKIRDVYRLLDDALGRHLAHVGDDDLVLFISDHGFQSCVRALHMDRF
                     LEHLGFLKFAASSAVFGPMQWGRMRALSRRIYDLLGLHGKVSLPQSVNWKKSVAYTAV
                     RSTGEGVSINLAGRESEGIVDPADFEATREKVIDALSGFVDPATGRKPVRAIHKREEF
                     FSGRFADTAPDIIMEPAAGYSLTHAKTAIEDADWLSGDHRMDGVIAAAGPHVRTDAFE
                     RPAFLVDVAPTTLAVLGVAPFVKHDGQVLESVVGVERSTAAEAAAPSAAAEEHEDTGL
                     DADEAAEVEEHLRGLGYLE"
     gene            complement(4905..5600)
                     /locus_tag="VF097_01175"
     CDS             complement(4905..5600)
                     /locus_tag="VF097_01175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HEX6261437.1"
                     /translation="MAREEQEPLYPSTVLSSDREVSGVRSAGWLILDAWRRRPVPRRV
                     LGTISVFLLLGGLTLVFYPQLTNLYGDWRQSRLEASFIEPETTEAYAARAVLPGEALT
                     KVEIPRLGLESIVVEGTSPGALRAGAGHYEGTALPCEVGNAAMAGHRTTYSKPFADLH
                     RLRPGDAIVLVTPVGRCVYEVAGSWVTTPDDVSVLADIRGASMLTLTTCHPPGSARER
                     LIVRAELVSSEAA"
     gene            5718..6278
                     /locus_tag="VF097_01180"
     CDS             5718..6278
                     /locus_tag="VF097_01180"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX6261438.1"
                     /translation="MLEFAILGLLQERSMHGYQLKQRLADALGPFWQVSYGSLYPALK
                     RLQKQGAVEMVFPRAEMGRRKNVYRITEHGQELFAELGESTQEDSDDNGFRVRLAFFR
                     HLKPEARIGVMERRRAVLVDRLRELKDRLASYRERVDGYTLSLMRHGMDTTEHDIRWL
                     DDMIQAERRASRSGRKRRAPKKGVKA"
     gene            6275..7402
                     /locus_tag="VF097_01185"
     CDS             6275..7402
                     /locus_tag="VF097_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014743497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX6261439.1"
                     /translation="MSKVRLAIVGVGNCASSLVQGLEFYKDADPAQQVPGLMHVRFGP
                     YHVSDVEVVAAFDVDGKKVGRDVSEAIFAEPNNTIRFSDVPPTGVEVLRGPTLDGFGR
                     YYREVAEESDLEPVDVAEALREAEADVVISYLPVGSEQAARHYAQAAIDAEAGFVNCM
                     PVFIASDRAWAKRFGDARLPIVGDDIKSQVGATIVHRMLAKLFEDRGVVLDHTSQLNF
                     GGNMDFMNMLEGERLASKKISKTQAVTSQLRDGIPAEDIHIGPSDHVPWLKDRKWAHI
                     RLEGREFGGMPISVELKLEVWDSPNSAGVAIDAIRSAKLAKDRGVGGPLIGASAYLMK
                     SPPVQFSDDAARDMVEEFILGNGGSRDQDWTKFFDVQPQES"
     gene            7458..8918
                     /locus_tag="VF097_01190"
     CDS             7458..8918
                     /locus_tag="VF097_01190"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6261440.1"
                     /translation="MSGRARLPGLRDLSGQRGFRRLIASQVTSQAADGLYQIAIAAVL
                     IFGVDAAETPGQITKVLAVTLLPFSLVGPFTGPFIDRFSRRSILTGTSVARAALTFAL
                     IPATGWPEWIILSLVVANMSVNRFFHATKSSALPTLVPRHRYLLANTVSSVGGMVFGL
                     AGAVVGGPVADAASAEMVILAAGVLMTVAAIVAATVPLPPGERKGLAGLLGELRDDAR
                     DVRDGLGLLLGTPRAVYAIASIWSIRGLIGFLLLAGIVLLRRRFGLETTGASLLLGAI
                     GIGGFLGALLVTPAARRLGDVRVAPLAYLVAGVTVLALGAIPAWPALVTLVAVGGAAM
                     AMTKITADTMVQRAIPDSYRGRAFAMYDIGYNGVFVLAALIPTLLIGLLGDIGIVFLA
                     GTLAVIGAGMFALASRRLREIEVRAYAGGRADETPREVVIDGTRLTVAEVERSWHEER
                     AGRRLLVFVLRMEDGRRLQLSRGEKWRIDRFLGEPG"
     gene            complement(8915..9301)
                     /locus_tag="VF097_01195"
     CDS             complement(8915..9301)
                     /locus_tag="VF097_01195"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HEX6261441.1"
                     /translation="MRRRGALLALVAVAAWLAWRRSFRVVVEGESMAPALRHGDQLLC
                     VRRGRIRRGDVVIVRAPGRSFEVVKRVTGIPGDPMGERTLGEEEYQVEGDDPLRSTDG
                     RSFGPVARHEILGVAVLRYLPEPRFL"
     gene            9389..>9512
                     /locus_tag="VF097_01200"
     CDS             9389..>9512
                     /locus_tag="VF097_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Ni]"
                     /protein_id="HEX6261442.1"
                     /translation="MLSRLLRPRRVVRAHCDLPCGVYDPAQARIEAESVQKIQEK"
BASE COUNT         1446 a         3403 c         3310 g         1353 t
ORIGIN      
        1 tggtgggccg tccccgacga gacgatcgag cgggcggggg tgacgccctc ggccctcccc
       61 ggggcggcgc acgccgcaga gcacgcggcc atcggcctcc tccccctcgt cgccacctgc
      121 gaccggtggg acatcggcgg cgtctcaacc gcccaccatc ccgacacggg ctcgtgcgcg
      181 atcttcatct acgacgggta cccaggcggg gccggcatcg ccgagcgggg cttcgcatcc
      241 ggcgagcggc tgctcagggc caccctggag acgatccggg cgtgcccgtg ccagagcggc
      301 tgcccctcgt gcgtccagtc ccccaagtgc gggaacggca acgagccgct ggataaggac
      361 gcggccgcgg ccctgatcga ggagatgctg gcgtgatccc gatccagatc gccgtctgcg
      421 ggcccggcct cgccaccgac gaggaggagc gctgggccga ggaggccggc cgccggctgg
      481 ccgaggaggg ggccgtcctg ctctgcggcg gcgtttcggg ggtgatggac gccgccgcgc
      541 gaggtgctcg cgaggccggc gggctcgccc tcgggatcct cccaggccgc gacctcgaga
      601 gcgcgagcga gcacctctcc gcggcggttg tcacggggat gggcgaagca cggaacgcgc
      661 tcgtcgtgcg gtcggccgac gcggtgctag cgatcgccgg ggagtacggc acgctctccg
      721 agatcgcgct cgccctgaag atgggcgtgc cggtcgtggg gctccacacc tgggaactgg
      781 cgaagggcgg ccggcccgtg gacgccttcg tccgcgccga cggtgttccc gaagccgtcc
      841 aggaggcgat gcggctcgcg tgggagcgac ggacgtgaag cgccgggtcg tgaacgccct
      901 ggccaagcac gtggccaacc ccgtgatgcg gcggctcgcg ggacgcatcc cgggctgggc
      961 cctgctggag accaccggtc gccgcagcag ccgagcacat cggatccccg tcggcgacgg
     1021 cctccgcggc gacgccttct ggatcgtcgc ggagcacggt cggcacgccg actgggtccg
     1081 caacgtcgag gcggacccca gggtgaggat ccgggtccga ggacggtgga ggaccggtgc
     1141 cgcgcacatc cttgaggacg acgacccgag ggagcgcctc cgccggctcg gaggcgtcaa
     1201 cccctgggtc atccgagtcg ccgggaccga gcccctgacc gtccgagtgg acctcgaccc
     1261 ggccccccct cgccccggat ccatccttcg ggacggcctt gtggctggcc tcgtagccgg
     1321 cgcggtagcc ggggcgccgt cgggcctcca cgccctccgc cacggctacc tcctcgacct
     1381 tctgcgggcg cccggtgctc tcgttgcgcc ccgagccggg gccgcgaccc agctctcgct
     1441 caccgtcccg gtccatgccg cgatctccgc cggctggggc atcgcgctgg cgaccgccct
     1501 cccccgccga gccaccgccc tgtggggggc ggcggggggc ctggtcgtcg cggcgctgga
     1561 catcgtggtc atcggaagcc ggatcccggt catccggagg ctcccactct ggccgcaggt
     1621 cgccgaccac gtcgcgttcg ggaccgtcgc cggcgggatc ctcgccggac tcagggaccc
     1681 cggctagccg cggacggccc ccaccagtct ggccagcacc cgccgctccg cgggcggcag
     1741 gccgaacgcg accagcacgg tgacgtacac cagcgtcccg atcccaccgg tgaccgccag
     1801 atccaccggc cacgacgcgc ctgccacgag gacgtgggcg cccagggccg cgccgagccc
     1861 gagggtcagc ggtacggcca gcaacgcgta gtggcgagtg aacggctgca tccgcaggaa
     1921 cctccacacc agccacaggc gagccacctt cgagagggcc atcgtcgcgg cgccggcgat
     1981 cgccgctccc tcgatgccca gccccagccc cccgatgagg agcgccgccg ctccgatgtc
     2041 gaaggcgacc gacgccgcgt agacgacgag gtcccatccc gtcctgccga tcatgatgag
     2101 cacgaacccg acggcgccgg tggcgacgtt gacgagctga cccaggagga ggatccccag
     2161 ggcggtggcc ccggcctcga actccgcgcc gaagagccgg agggcagact cgggcgccgc
     2221 ggtgagcacg atgaacaccg ggagggtcgc ggccagggtc cagcgggtga gctgcttgta
     2281 gagccgatca agcttctccc gctcgccccg ggcgtgcaga tccgccacga aggggctgaa
     2341 catcaggctg acggagatga tgaacagcag cagcacctgc cccgcccgcg ccgccgccga
     2401 atagatcccc acctcccgag aggaggcgaa gacggcgagc acgaagacgt cggtccagaa
     2461 gagcagctgc gagaagaggg cagccggagc cctgggggcc ccataccgca tcagatcccg
     2521 gacctccccg gaaaggggag ggtcgccgcc gaacccccgg gtctcccgat accagagcca
     2581 agctgccgcg gccgtggcgc ccgcccagga gacggtgtag gcgagggtcg tcactcctac
     2641 ggacctggcc acgagccagc ccgccaggat cagggcgaac cacgccagcg gctggcccat
     2701 ccagaagacg tacagggtgt ggcgcatgat cttcagccca cgggtcgccc ccaggtagac
     2761 ctggaccagc gcggtgaacg ggatcgcgag ggctgccgcc cggaacgccg cggtggccgc
     2821 gcccgccgcg tcggcggcca gcgagcgcgc gatgggctcg gcgagcagga acacggcggc
     2881 ggccacgatc aggctcgaga ccatcgagat gatcgatgcg cgcgtgacca ccgcgcgcac
     2941 cctcgagcgc tctccccgtc ccacgtcgat ggccacgcgt cgaacggccg ccatgtccat
     3001 cccgaagcga gagaagtatc cgaggaccag cgcgctctgc gttgccagcg tcacgatgcc
     3061 gaaggccgag gcccccagcg ccctcgtgat gagcacctgg gtgccgaagg tggccacgat
     3121 cgcgaccacc atcccgacca cgttctggcc cgtgccggcg aggatggtcc gctcgtcgcc
     3181 gaagcgttcc agcgggagca ccgactccag ggcgctgcgt atccttcggg ctcgcttccc
     3241 gctattcgag gtagccaagg ccccggaggt gctcctcgac ctcggccgcc tcatcggcgt
     3301 cgagccccgt gtcctcgtgc tcctccgccg ccgccgacgg ggccgccgcc tccgcggcgg
     3361 tcgagcgctc caccccaacc accgactcca gcacctgccc gtcgtgcttg acgaagggcg
     3421 caacgccgag cacggcgagg gtcgtcgggg ccacgtccac caggaaggcc ggacgctcga
     3481 aggcgtcggt ccgcacgtga ggaccggccg ccgcgatcac gccgtccatc cggtggtcgc
     3541 ccgagagcca gtccgcgtcc tcgatcgctg tcttcgcatg cgtgaggctg taacctgcgg
     3601 ccggctccat gatgatgtca ggggccgtgt ccgcgaacct cccggagaag aactcctcgc
     3661 gcttgtggat cgccctcacc ggcttccgcc cggtggccgg gtcgacgaac cccgagagcg
     3721 cgtcgatcac cttctcgcgc gtggcctcga agtcggcggg gtccacgatc ccctcggatt
     3781 cgcggcccgc gagattgatg gagaccccct cgcccgtcga gcgcacggcc gtgtaggcga
     3841 cgctcttctt ccagttcacg gactgcggca gcgacacctt gccgtggagc cccagcagat
     3901 cgtagatgcg gcgtgagagc gcgcgcatcc tcccccactg catcggcccg aagaccgcgc
     3961 tcgacgccgc gaacttcagg aagcccaagt gctcgaggaa gcggtccatg tgcagggccc
     4021 tgacgcacga ctggaagccg tggtcggaga tgaacagaac gaggtcgtcg tcgcccacgt
     4081 gcgccaggtg gcgtccgagg gcgtcgtcga gcagccggta gacgtccctg atcttctccg
     4141 cgagcggctc cttcgacagc gtggagaagt ccggatggtc ggaggagacg tacttcgcga
     4201 gacagtgctg cacacggtcg gtcgccacgt agacgagcga agcgaacctc cagtcggtgc
     4261 tcgccaggag atgctcgttc gcctcgatcc gctgacccgt cagctccatg gcctccgcca
     4321 ggaagggctc gggtcggttc cagtaggtcg tccacgacat cccgttgatc gggaacggct
     4381 tcccggccgc ctcgagctcc gcggccagcc cctctgggtg cgtgtagggg cggcccttgg
     4441 gcaacacgcc gcccgagacc agcttcccct ggatcctctg gggcgggaag gtgaggggga
     4501 cgttcgtcat gatcgtctcg accccggcgg ctgtgaggtc gggaagcatc gtcctctcct
     4561 tgatcgatcc gtaggtcacg ggcagctgct tccgcatctc gaagttccgc acgaagatgt
     4621 cgtagacgcc gtggttcgag ggctcgaccc ctgtgaggaa ggacggccag gcggaccagg
     4681 agtggttggg gaccgtggac ttcaggacgc ccctcgcccc gcggtcgatg agcgatcgaa
     4741 ggttcgggag aaggcccgct tccagcatgg gatcgaggat gcgccagtcc gcgccgtccc
     4801 agccgatcag cagcactctt gacatgcgga tcctttcgga gtggtcggtg cccgtgaagg
     4861 cgactcgtta gtgtaattgg cctcgtggcg ggctgaccag cggctcacgc ggcctcgctc
     4921 gagacgagct ccgcccggac gatcagccgc tcccgcgcgc tccccggcgg gtggcaggtc
     4981 gtcagggtga gcatcgacgc gccgcggatg tcggccagca cgctcacgtc gtcgggggtc
     5041 gtgacccacg accctgccac ctcgtacaca caccgcccca cgggggtgac cagcacgatc
     5101 gcatcccccg gtcggagcct gtggaggtcg gcgaacggct tcgagtacgt cgtgcggtgc
     5161 ccggccatcg ccgcgttccc gacctcgcac gggagcgcgg tcccctcgta gtggcccgcc
     5221 cccgcacgca gcgccccggg ggacgttccc tccacgacga tcgactcgag ccccaggcga
     5281 gggatctcga ccttcgtcag cgcctctccg gggagcactg cccgggccgc gtaggcctcc
     5341 gtggtctccg gctcgatgaa cgacgcctcg agcctcgact gacgccagtc cccgtagagg
     5401 ttcgtgagct gggggtagaa gaccagcgtg aggcccccga ggaggaggaa gaccgagatg
     5461 gtccccagca cgcgacgtgg gacgggcctg cggcgccagg cgtcgaggat cagccacccg
     5521 gcggaccgga ccccgctcac ctcccggtcc gaggagagca cggtggacgg gtagagcggc
     5581 tcctgctcct cccgagccat gggccggagt ctatccccgc cccgaggggc gcgatgttga
     5641 cactccgccg gatcgtgggt atatatcgag gcgatatatc gacccgatac atcgagccga
     5701 tacatggaag gagagaggtg ctcgagttcg ccatcctagg gcttctccag gagcggtcca
     5761 tgcacgggta ccagctgaag cagcggctgg ccgacgccct gggccctttc tggcaggtct
     5821 cctacggctc cctctacccg gcgctgaagc ggctgcagaa gcagggcgcg gtcgagatgg
     5881 tgttccccag ggccgagatg ggccgccgca agaacgtcta ccggatcacc gagcacggcc
     5941 aggagctgtt cgccgagctc ggagagagca cccaggagga ctccgacgac aacggcttcc
     6001 gtgtcaggct ggcgttcttc cgccacctga agccggaggc gcggatcggc gtgatggagc
     6061 gccgacgcgc cgtgctggtc gatcggctcc gcgagctgaa ggaccggctg gcctcgtacc
     6121 gcgagcgcgt ggacggctac acgctgtcgc tgatgcgcca cggcatggac acgaccgaac
     6181 acgacatccg ctggctcgac gacatgatcc aggcagagcg cagggccagc cggagcggcc
     6241 gcaagcgccg tgctccgaag aagggagtca aggcatgagc aaggtccgac tggccatcgt
     6301 tggggtgggc aactgcgcct cctccctcgt gcagggtctg gagttctaca aggacgccga
     6361 ccccgcccag caggtccccg ggctgatgca cgtccggttc ggtccctacc acgtctccga
     6421 cgtggaggtc gtcgcggcgt tcgacgtaga cggcaagaag gtagggcgcg acgtctccga
     6481 ggcgatcttc gcggagccca acaacacgat ccggttctcc gacgtcccgc ccacgggcgt
     6541 ggaggtcctc cgcggcccga ccctcgacgg cttcggccgc tactaccgag aggtggccga
     6601 ggagtcggac ctcgagccgg tcgacgtggc cgaggcgctc cgcgaggctg aggcggacgt
     6661 cgtcatcagc tacctccctg tggggagcga gcaggccgcg cgccactacg cgcaggccgc
     6721 gatcgatgcg gaggcgggct tcgtgaactg tatgcccgtg ttcatcgcct ccgaccgcgc
     6781 gtgggcgaaa cgcttcggcg acgcccgtct ccccatcgtc ggggacgaca tcaagtccca
     6841 ggtcggggcc acgatcgtcc accggatgct cgccaagctc ttcgaggacc gcggcgtagt
     6901 gctcgaccac acgtcccagc tcaacttcgg cgggaacatg gacttcatga acatgctgga
     6961 gggcgagcgg ctggcctcga agaagatctc caagactcag gccgtcacct cgcagctgcg
     7021 ggacggcatc ccggccgagg acatccacat cggcccctcc gaccacgtgc cgtggctgaa
     7081 ggaccggaag tgggcccaca tccggctcga gggtcgggag ttcggcggga tgcccatctc
     7141 ggtggagctg aagctcgagg tctgggactc gccgaactcg gctggcgtgg cgatcgacgc
     7201 gatccggtcg gcgaagctgg ccaaggaccg aggcgtgggt ggcccgctga tcggtgcttc
     7261 cgcctacctg atgaagtccc cgccggtgca gttctccgac gacgcagccc gcgacatggt
     7321 cgaggagttc atcctcggca acggcggctc cagggatcag gactggacga agttcttcga
     7381 cgtgcagcct caagagagct gagcgggaga ccctcgggag cgcctccccg aggatcgtcc
     7441 ccgcctaaac tctggggatg agcggacgtg cgcgcctgcc cggcctccgc gacctatcgg
     7501 gacagcgtgg cttccggcga ctgatcgcct cgcaggtcac ctcccaggcc gccgacggcc
     7561 tctaccagat cgccatcgca gccgtcctga tcttcggcgt ggacgcggcg gagactccgg
     7621 ggcagatcac caaggtgctc gccgtcacgc tgctgccctt ctcgttggtc ggtcccttca
     7681 ccggcccgtt catcgatcgc ttctctcgac gctcgatcct cacggggacg agcgtcgccc
     7741 gggccgccct caccttcgcc ctgatcccgg ccacgggctg gccggagtgg atcatcctct
     7801 ccctggtggt cgccaacatg tcagtgaacc gcttcttcca cgccacgaag agctccgccc
     7861 tccccacgct cgtcccgcgg caccggtacc ttctggcgaa caccgtgtcc tccgtcggag
     7921 ggatggtgtt cgggctggcg ggcgcggtgg tgggcggacc cgtcgccgac gccgcctccg
     7981 ccgagatggt gatcctggcc gccggcgtgc tgatgaccgt cgcggcgatc gtcgcggcga
     8041 cggtccccct ccctcccgga gagcggaagg gcctggcggg gctgctgggg gagctccgag
     8101 acgacgcacg ggacgttcgg gatgggcttg gtctgctcct gggtaccccc cgggccgtct
     8161 acgccatcgc ctccatctgg tccatccggg ggctgatcgg gttcctcctg ctggccggga
     8221 tcgtcctgct ccggaggagg ttcggcctgg agacgacggg cgcctccctt cttctggggg
     8281 cgatcggcat cggcgggttc ctgggggcgc tcctggtgac ccccgcggca cgccggctgg
     8341 gcgacgtccg cgtcgcgccc ctcgcgtacc tggtagccgg ggtcaccgtc ctggcgctcg
     8401 gcgccatccc cgcgtggccg gcgctcgtaa cgctcgtggc cgtgggcggc gccgcgatgg
     8461 cgatgacgaa gatcacggca gacacgatgg tccaacgggc aatccccgac tcctatcggg
     8521 gacgggcctt cgccatgtac gacatcggct acaacggcgt cttcgtcctc gcggccctga
     8581 tccctacgct cctcatcggg ctcctgggcg acatcgggat cgtcttcctg gccgggaccc
     8641 tcgcggtgat cggggcaggg atgttcgccc tcgcctcgcg aaggctccgc gagatcgagg
     8701 tgcgcgccta tgcgggaggc cgggccgacg agaccccgcg cgaggtcgtg atcgacggaa
     8761 cgaggctgac ggtggcggag gtggagcgct cctggcacga ggagcgggcg ggccggcgcc
     8821 tcctcgtctt tgtcctgcgc atggaggacg gcaggcgcct ccagctcagc cgcggcgaga
     8881 agtggcggat cgaccgcttc ctgggcgagc ccggctagag gaaccggggc tccggcaggt
     8941 accggagcac ggccacacct aggatctcgt ggcgagccac cggcccgaac gaccgtccgt
     9001 cggtggatcg cagggggtcg tccccctcga cctggtactc ctcctcgccc agggtccgct
     9061 cccccatcgg gtcaccgggg atcccggtca cccgcttcac cacctcgaag ctgcggcccg
     9121 gcgcccgaac gatgaccacg tcgccgcgcc ggatccttcc ccgtcgcacg cacaggagct
     9181 ggtcgccgtg tcgcagcgcg ggggccatgc tctcgccctc gacgaccacc cggaacgacc
     9241 ttcgccatgc cagccacgcg gcgaccgcga cgagcgccag cagcgcccct cgcctccgca
     9301 tgtgcgaagc ctacccgggt aggaaggtgt gtggccgctc gcgacgagcg gatggtagcg
     9361 tgagagccca tcagaggaag gaggaggcat gctcagccga ctgctgaggc ccaggcgggt
     9421 cgtccgcgcg cactgcgacc tcccctgcgg cgtctacgac ccggcccagg ccaggatcga
     9481 ggcggaatcg gtccagaaga tccaggagaa gt
//
LOCUS       DASUTZ010000026         2548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1820909, whole genome shotgun sequence.
ACCESSION   DASUTZ010000026 DASUTZ010000000
VERSION     DASUTZ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1820909"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1555
                     /locus_tag="VF097_01205"
     CDS             <1..1555
                     /locus_tag="VF097_01205"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HEX6261443.1"
                     /translation="SSWPRRSPGSCIAMTDADERTVILAYHEATKHSPVSVRARAHFL
                     DWDNRPFSFKVYTDLERISLPEDIPSLDVPALEAIGVTSVPDPMPGPDLAALARILVL
                     GAGVHHTKTFREGEVPYFRNYASAGALYPVEVYVGCADLPGLPAGVYHFDPQGPTLTP
                     LREGDHRGHLVRAAAAEPAVARAPVVLALTGIPWRTAWKYTERGYRHLFWDAGMILAN
                     VLALAASVRLPVRVVLGFADAEVSVLLGLQERREFPLCLVAIGAADSETPPAAQPPAE
                     VSFRVLPLSRTEHIYQGILQANDAGSLDVPKEVGTWRRTVFTREQVEPAPPLSSDTAP
                     DSLEDVLRRRGSARIFAPGAIPATVLRTILERATVGIPTDYATGGARLVEPYLIANEV
                     QGLDPGAYVFRDGELRLLDRGDFRREAGFLCLEQELGATAAATHFLMADLPRILDALG
                     GRGYRAAQLEAGIVAGRLYLGAYAYRFGATGLTFYDDAVTEFFSPDAAGKSCVLVTAI
                     GESPRLRRA"
     gene            1707..>2548
                     /locus_tag="VF097_01210"
     CDS             1707..>2548
                     /locus_tag="VF097_01210"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6261444.1"
                     /translation="MAADTLLLAGSLSLTGRYAPQGRLAAAGLHQAVQDVRNRIGVRM
                     AHRHLVPDVVILDDASTREGVRRSLDRVSGADLLVGPYGSDLVAEAARWAADRGRALW
                     NHGGNADDVQRLPGVVSVGTPASRYLSSVLDAVATELPEARVMVAVGRGAFGRSIAHG
                     AREAAERLGMTIVAITTHAEVPEAPNADALLMAGTFAEDLALVRRLRARPPAVGAVAA
                     GLGMFRAELGPMAEGILGPSQWEEGLHFPIDVGPRQAEVMRSLRASVFPTLRAGARAG
                     HVEYP"
BASE COUNT          353 a          943 c          868 g          384 t
ORIGIN      
        1 gtcgagctgg cccagaagat cccccggctc ctgcatcgcc atgactgacg ccgacgaacg
       61 gaccgtgatc ctcgcctacc acgaggcgac gaagcactca ccggtcagcg tgcgagcccg
      121 cgcgcatttc cttgactggg acaaccggcc cttttccttc aaggtctaca cggacctcga
      181 gaggatctcc ctgccggagg acataccctc cctcgacgtc ccggccctgg aggcgatcgg
      241 cgtcacctcc gtcccggacc ccatgccagg cccggacctc gcagccctgg cccgaatcct
      301 cgtcctcggc gccggtgtcc accacacgaa gacattccgc gagggcgagg tcccgtactt
      361 ccgcaattac gcctcggccg gggccctgta cccggtcgag gtctacgtgg gctgcgccga
      421 cctcccgggc ctgcccgcag gggtgtacca cttcgacccg cagggcccga ccctcacccc
      481 ccttagggag ggcgaccacc gcggtcacct tgtccgagcg gctgcggcgg agcctgccgt
      541 ggcccgggcc ccggtggtgc tcgccctcac cggcatcccc tggcggaccg cgtggaaata
      601 caccgagcgg gggtaccgcc acctgttctg ggacgccgga atgatcctgg cgaacgtcct
      661 cgccctggcg gcctcggtcc gcctcccggt ccgcgtcgtc ctggggttcg ccgatgccga
      721 ggtctccgtg ctgctcgggc tgcaggagcg acgcgagttc cccctctgcc tggtcgcgat
      781 cggcgccgct gactccgaga ccccacccgc cgcgcagccg cccgcggagg tctcgttcag
      841 ggtcctgccg ctctcacgga ccgagcacat ctaccagggc atcctccagg cgaacgacgc
      901 tggcagtctg gacgttccaa aggaggttgg gacgtggcgg cgcaccgtgt tcacccgcga
      961 gcaggtcgag ccggctccac cgctctcatc cgatacggcg cccgactccc tcgaggacgt
     1021 cctccgccgg cgcggctccg cccggatctt cgcgcccggc gcgattcccg ccactgttct
     1081 ccgaacgatc ttagagcgag ctacggttgg catcccgacc gactacgcga ctgggggcgc
     1141 ccgtctcgtg gagccgtacc tgatcgcgaa cgaagtccag ggcctcgatc cgggcgctta
     1201 cgttttccgc gacggcgaac tccggctcct ggaccggggc gacttccgcc gggaggccgg
     1261 gttcctctgc ctagagcagg agctcggcgc gacggcggca gcgacccact tcttgatggc
     1321 ggacctcccg aggatcctcg acgcgctcgg aggccgcggc taccgcgccg cgcagctgga
     1381 ggccggcatc gtcgccgggc gcctatacct gggcgcgtac gcgtaccgtt tcggtgccac
     1441 cggcctcacg ttctacgacg acgcggttac cgagttcttc tcgcccgacg ccgcgggcaa
     1501 gagctgcgtg cttgtcacgg cgatcggcga gagtcctcgc ctccgacgcg cctgatccgg
     1561 cggcaagtca gggaaggccc tctgcggcgg ccctggtccg gaccggcgcg cccctatgat
     1621 cgaagcggcg ccgaagatcg agggactcct cgccgtcgac gccatacccg cgagaccgag
     1681 gccgaccggt catgatcagc gcgagcatgg cggcggacac acttcttctg gccgggtccc
     1741 tgtcgctgac cgggcggtac gcaccacagg ggcgactcgc cgcggccggc ctgcaccagg
     1801 ccgtgcagga tgtccgcaac cggatagggg tgcgcatggc ccatcggcac ctggtcccgg
     1861 acgttgtgat cctcgatgac gcgagcacgc gagagggcgt tcgccgctca ctcgaccggg
     1921 tatcgggagc ggacctcctg gtgggcccct acggcagcga tctcgtcgcc gaggccgcac
     1981 ggtgggcggc ggaccggggg cgggccctgt ggaaccacgg agggaacgcc gacgatgtgc
     2041 agcgcctccc cggggtggtg tcggttggaa cgcccgcgag ccggtacctc tcctcggtcc
     2101 tcgacgcggt ggccaccgag ctccccgagg cccgggtcat ggtggcggtc gggcgcgggg
     2161 ccttcggccg cagcattgca catggtgcgc gggaagcggc ggagcggctc ggcatgacga
     2221 tcgttgcgat cacgacccac gccgaagtgc cggaggcacc caacgcagac gcgttgctca
     2281 tggccgggac cttcgcggag gacctcgcgc tcgtccgtcg cctccgcgct cgaccccccg
     2341 cggtcggagc ggtggcggcc gggctcggca tgttcagggc cgagctcgga cccatggccg
     2401 aaggcatctt gggaccctcc cagtgggagg agggtctgca cttcccgatc gatgtcgggc
     2461 cccggcaggc ggaggtgatg cggagcctcc gggccagcgt cttcccgacg cttcgagccg
     2521 gggcgagggc cggccacgtc gagtaccc
//
LOCUS       DASUTZ010000027        10729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1173235, whole genome shotgun sequence.
ACCESSION   DASUTZ010000027 DASUTZ010000000
VERSION     DASUTZ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10729
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173235"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="VF097_01215"
     CDS             complement(<1..879)
                     /locus_tag="VF097_01215"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEX6261445.1"
                     /translation="MKPLVKPPPLPPGGTIGVPAPSSPWQDRSEWDRGIRWWESKGYA
                     VKLAKNAMARDDYVAGDPVDRARDVEAMFADPEVDVVQVFGGGYGSSQVVPHLDTGVI
                     ASNPKPFMGYSDITALHVAIRQATGLVTFHGPGLAGIAYEASEDRQFTQDRVLRALTD
                     PAPLGEIPARPDDPYVGAIGSGVARGPIVGGNLWMIRETLGTAWEIETAGCVLFFEDV
                     YTPPWHVDSMLTHLANVGKLREVVGVVVGEMYRSEEWRHPEPWLRSRSMEDVLEYHLG
                     PLGVPVLTNLPLGHGKH"
     gene            complement(876..1676)
                     /locus_tag="VF097_01220"
     CDS             complement(876..1676)
                     /locus_tag="VF097_01220"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX6261446.1"
                     /translation="MRTSPVRYERLTWPEVAEAARPDAVCLIPVATLEDHGPHLPIDA
                     DLRIVAEIAERAASATGAILLPAIPHGYSPHHLDFPGPITIGWDTFTRYVRDVGISLA
                     DHGFRRMLFLNGHGSNQNLVETAARLLMVERPETLAAAAFYLTSEKGLAEVERIRESE
                     RGGMAHACELETSIYLAIEPEAVAMDLAVDEMSYPAGDHAWIDWTDGPLKLMPWWSSF
                     SETGVQGRPTLATAEKGQALLDAAVAEVSAYLEELRAKPLPRRREPSA"
     gene            complement(1676..2704)
                     /locus_tag="VF097_01225"
     CDS             complement(1676..2704)
                     /locus_tag="VF097_01225"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEX6261447.1"
                     /translation="MIPVVRVSGGPEERGRAVGEALAEPIHRSLAFYRGYLQRRGISR
                     ESFPLVLRPHRRAAEQGLPELVAEIDAMAEGAGADPWDLFAVNAFEELEPAMSEPSME
                     RCTSFSASTPGGPILAHNEQWLAGDLGNVAVVVADPTEGPGFASPTVVACLPAVGMNT
                     AGLAQAIMSLSAGDDREGVPRVLVSRHALQARFSDDAVKRADLPGRAGGYAHMFAGGG
                     GAAFTIETTAERVAVLDGHGPHTNHYIHQDLTLIGDPPRAGSLSRLERARELLEDRPP
                     QSPEDAMAILADHEGHPQPICLHADPKDGDDAEAVVFSMVCSLHDRRMWVAPGPPCST
                     PFEEIEVA"
     gene            complement(2701..4080)
                     /locus_tag="VF097_01230"
     CDS             complement(2701..4080)
                     /locus_tag="VF097_01230"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX6261448.1"
                     /translation="MGGDQAAAIVGERFDTYLERIRDLCRMPSVSATGEGVRDVADAV
                     LGMIQAAGGEAELVETPGFPVILGEIPGPPGAPSLLRYGMYDVQPAEEPDWTVPPFSA
                     EIVDLDGIGPSVVCRGVANSKGSLASFFCALDALRDAGGIPCAIRFVIEGEEELGSMN
                     LPAVVTERADDLRADCGADLDLCGERDGSAQLITGCKGLLSMELVCRAGDWGGPAKPL
                     HSSEAAWIASPVWSLVHALATIVGPGEELLVHEITAAAAPPSDDDRPLLRELAAGFDP
                     AEHLGDARASRYKIEGTPEELLEALIYRPTANIDGLEAGYTGPGHTIMPSEARAVLDL
                     RLVPDLDPDEAAAAVRRHLDREGFPHVEVLPLERYPWARSAPDSRASRAMRASYDRLG
                     RRPLPYPMAPWCAPFYIFDRLLEMPWASGGLGFSGGAHASDEFATVDGLREHILGTVG
                     FLLAYAEAA"
     gene            complement(4333..5211)
                     /locus_tag="VF097_01235"
     CDS             complement(4333..5211)
                     /locus_tag="VF097_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEX6261449.1"
                     /translation="MSEPTRSPELRGAERPLVGLETSVLAQGLPEPRNREAADRMGEA
                     VRAAGALPAWTWVDDGEVRVGATGDELDRLCAEAAAKVARRDLPMAVAGRGLGATTVS
                     ATLWIAAHAGIEVAATGGIGGVHHGGGDVSADLAELARSPVTLVCAGPKSITDPAATL
                     ERLEELGVGVVGYGTDRLPFFLAREAPIELEHRADSPREAAEIARARRDLGLGAALLV
                     CVPIPELYALDPDEIADAVAGCEERAAREAVNGKDLTPYLLRCLAQETDGRSLEANLA
                     LLEENARVAGAIAAAL"
     gene            complement(5208..6065)
                     /locus_tag="VF097_01240"
     CDS             complement(5208..6065)
                     /locus_tag="VF097_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEX6261450.1"
                     /translation="MTDLAVVGDALLDIAAHAPPLLEGGDVRGTVRARPGGQGANVAA
                     WAASEGLRVRLHARVGDDAAGRLVRESLEARGVETALVVDPGAPTGTLLVLVHGGDRS
                     MVADRGAAARLSPADLPEEIEADAVLVSGYLLFDQGSEAAGLAALEQARARHLAVETS
                     TWPLLEAYGAERFLEATSAATVLLANEREAEVLTDAEGEEAAAKLGERYEVAVVKLGD
                     RGAALASAELTLLEPAKQVEVADPTGAGDAFDAAFLATLLRGGSLGRALDHAITTGGW
                     VASREEAWP"
     gene            complement(6062..7309)
                     /locus_tag="VF097_01245"
     CDS             complement(6062..7309)
                     /locus_tag="VF097_01245"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6261451.1"
                     /translation="MARPKVLVLGGGYAGVMAAAGLGARADVLIVSRDNFLLFTPMLA
                     EVAAGDLDPRHVVSPIRQLAPHARLLIGDIEEIDLASKTVTVRPKLREGVVRYGADAI
                     VLGLGAVQATFGIEGVDEHALPFKTISDALRIRNRMLASLEAAAESHDPRLTRVAVVG
                     AGYSGAELATALADMLSEAAARFYRTAPRPHVTLVDALERVTAMLPERLSAEAERGIR
                     ERGIEPVLGAKVAKVHEGGISLEDGREIEAGTVIWAAGVRPNPLAETLGLPAGPGGRL
                     VVDGHLRAAPGVFAGGDLAAVPDGRGGISPPTAQFALRQGVYLGRHLLDVLEGRSAPP
                     FRYRTLGELVSLGHRNAVGLVFGVPVTGVPAWFLWRSYYLMRLPTLLRKARVALDWTL
                     DVVFPPDIAWLPTSDLGPDPSEP"
     gene            7367..7993
                     /locus_tag="VF097_01250"
     CDS             7367..7993
                     /locus_tag="VF097_01250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_4193 family putative phosphomutase"
                     /protein_id="HEX6261452.1"
                     /translation="MARILLIRHAVADHTGKRLYGRSQGVALSERGRADAEGLAERLR
                     RVRIHGLYVSPMQRCRETAETIARGRRLEPEILEDLNEVDYGDWTGRTFASLRRTKVW
                     RQVRTRPSTARFPNGESLLEVQERAVRAIEGIARRHRRGVAAVVTHGDVIRLALAHYA
                     GLHLDLFQRLEASTASVTALALGDGIPRVLRANDTGRLEDLVPGKVGG"
     gene            7998..8504
                     /locus_tag="VF097_01255"
     CDS             7998..8504
                     /locus_tag="VF097_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HEX6261453.1"
                     /translation="MELDPVDRITVGAVGEPGERTFYLQARRGTDLVTIVVEKQQVQL
                     LSATVVDMLSRLGTDVEVEVGEHEMELEQPLVPEWRAARIAVGYAEERDLFMLEAEEL
                     EEEDEEAELGGQARFWATREQMLALARHGIAVAAAGRPTCEFCGNPMDPEGHVCPAMN
                     GQGRLGEA"
     gene            8501..9184
                     /locus_tag="VF097_01260"
     CDS             8501..9184
                     /locus_tag="VF097_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HEX6261454.1"
                     /translation="MTTALAGGELEVLGLLPGASNHTFLGRIGGLHVVYKPVRGEAPL
                     WDFPIGTLAAREVAAARLAEALGWPDVPPTVMRDGPAGPGAVQEYVMAVEGEHYFTLR
                     EERAGELKAVAAFDVVANNADRKGGHVLLGEDGRLWCIDHGLTFHVEPKLRTVIWDFA
                     GEALPGGLAEDLERVVGELRGGPLREGLEGVLDPAEVGAAGARAEALLEAGRFPGPGP
                     GRPIPWPLV"
     gene            9184..9999
                     /locus_tag="VF097_01265"
     CDS             9184..9999
                     /locus_tag="VF097_01265"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6261455.1"
                     /translation="MGALERWREALASWAIPDEILAKAPEPPWGFRPEIFADRARRAP
                     EEPETPSRRRAGAALRGGGTVLDVGAGAGAASLPLAPPADAIVAVDPSRELLTAFEEL
                     AVEREVTLTTIERSWPEAAGESPVCDVVVCHHVLYNVPDLGPFVRALTDHARRLVVVE
                     LTAQHPVSWMADLWRRFHDLERPTEPTADVAVAALREAGIDTGREDHLVPREGGGFPD
                     REAAVAQARRMLCLTPDRDPEVAEALGDRLREGPTGWGIGPPAARVVTLWWEP"
     gene            complement(9977..10396)
                     /locus_tag="VF097_01270"
     CDS             complement(9977..10396)
                     /locus_tag="VF097_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HEX6261456.1"
                     /translation="MSTPLARKLGITAGSRVVFVGQPDGFEGALAPLPRGVTIATRVG
                     GPHDVVVLFAHRRSALERRLPSLRKALDYTGSLWIAYPKKASGVPSDLDFTVVQEAGL
                     ATGLVDNKTASIDETWTAVRFVYRLSDRPEVTAPTIG"
     gene            complement(10393..>10729)
                     /locus_tag="VF097_01275"
     CDS             complement(10393..>10729)
                     /locus_tag="VF097_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261457.1"
                     /translation="FHLAGRLLARHPRATRQVIMVTDGEPTAHLEGDEVFFSWPPVRE
                     TIRRTLSEAMRLARAGVTLNVFMLEDSPGLARFMERLARLTSGRIFLMSGESVGDFVL
                     RDYVARRAS"
BASE COUNT         1428 a         3869 c         4061 g         1371 t
ORIGIN      
        1 gtgcttcccg tgccccaggg gcaggttggt cagcaccggg accccgaggg gcccgaggtg
       61 gtactccagc acgtcctcca tcgagcggct ccggagccag ggctccggat gccgccactc
      121 ctcgctgcgg tacatctcgc ccacgaccac gcccacgacc tcgcggagct tccccacgtt
      181 ggcgaggtgg gtgagcatgc tgtccacgtg ccacggcggc gtgtagacgt cctcgaagaa
      241 gaggacgcag ccggccgtct cgatctccca ggcagtgccc agggtctcgc ggatcatcca
      301 gaggttgccg ccgacgatcg ggccccgggc gacgcccgag ccgatcgccc cgacgtaggg
      361 gtcgtcgggg cgggccggga tctcgcccag cggggcgggg tcggtgaggg cgcggaggac
      421 ccgatcctgg gtgaattggc ggtcctccga ggcctcgtag gcgatcccgg ccaggcccgg
      481 cccgtggaac gtgacgagcc cggtggcctg gcggatcgcc acgtggaggg cggtgatgtc
      541 ggagtagccc atgaagggct tggggttgga ggcgatcacg ccggtgtcga ggtggggcac
      601 gacctgggaa gagccgtagc cacccccgaa gacctggacc acatcgacct cggggtcggc
      661 gaacatggcc tcgacgtcgc gcgcccggtc cacagggtcg ccggccacgt agtcgtcgcg
      721 ggccatcgcg ttcttggcca gcttcaccgc gtagcccttc gactcccacc agcggatccc
      781 gcggtcccac tccgagcggt cctgccaggg cgaggacggg gcggggacgc cgatcgtgcc
      841 gccgggcgga aggggtggtg gcttcaccag gggcttcacg ccgagggctc ccgtcgccgg
      901 gggagtggct tcgcgcgaag ctcctccaga taggcggaga cctcggccac ggctgcatcc
      961 agcagggcct ggcccttctc cgcggtggcc aacgtgggcc ggccctggac gccggtctcg
     1021 gagaacgagg accaccacgg catcagcttc agcggcccgt cggtccagtc gatccaggcg
     1081 tggtcgcccg ccgggtagga catctcgtcc acggccaggt ccatggccac ggcctcgggc
     1141 tcgatcgcga ggtagatcga ggtctccagc tcgcaggcgt gggccatgcc gccgcgctcg
     1201 gactcgcgga tccgctccac ctcggccagc cccttctcgg aggtgaggta gaaggccgcg
     1261 gcggcgaggg tctccggtcg ctccaccatg aggagacgcg cggccgtctc cacgagattc
     1321 tggttggagc cgtggccgtt gaggaagagc atgcgacgga agccgtggtc ggccagcgag
     1381 atccccacgt cgcggacgta gcgggtgaag gtgtcccagc cgatcgtgat cggccccggg
     1441 aagtcgaggt ggtggggcga atacccgtgg gggatcgccg ggaggaggat cgcgccggtg
     1501 gccgaggccg cgcgctcggc gatctcggcc acgatccgca ggtccgcgtc gatcggcagg
     1561 tggggtccgt ggtcctccag ggtcgccact gggatcaggc agacggcgtc ggggcgcgcg
     1621 gcctcggcca cctcgggcca ggtgagccgc tcatagcgca ccggggacgt ccgcatcagg
     1681 ccacctcgat ctcctcgaag ggggtggagc agggcgggcc cggggccacc cacatcctcc
     1741 ggtcgtggag gctgcagacc atggagaaga cgacggcctc ggcgtcgtcg ccgtccttcg
     1801 ggtcggcatg gagacagatc ggctgagggt ggccctcgtg gtcggcgagg atcgccatgg
     1861 cgtcctcggg ggactgaggg ggacggtcct ccaggagctc gcgggcccgc tcgaggcggg
     1921 acaggcttcc cgcccgcggc gggtcaccga tcagcgtgag gtcctggtgg atgtagtggt
     1981 tcgtgtgggg accgtgcccg tcgaggacgg ccacgcgctc ggccgtggtc tcgatcgtga
     2041 aggccgcgcc gccgccgccg gcgaacatgt gtgcgtagcc gccggcccgt ccgggcaggt
     2101 ccgctcgctt gaccgcgtcg tcgctgaagc gcgcctggag ggcgtgccgc gagacgagga
     2161 ctcgcggcac gccctcccga tcgtcccccg cgctgagcga catgatcgcc tgggccagcc
     2221 cagcggtgtt catgccgacc gccgggagac aggccacgac cgtcggcgag gcgaagccgg
     2281 gcccctcggt cgggtccgcc acgaccaccg ccacgttccc caggtcgccg gcgagccact
     2341 gctcgttgtg ggcgaggatc gggccgcccg gggtggatgc gctgaaagag gtacaccgct
     2401 ccatcgaggg ctcggacatg gcgggctcca gctcctcgaa ggcgttcacg gcgaagaggt
     2461 cccacggatc ggcgccggct ccctcggcca tcgcgtcgat ctcggccacg agctcgggga
     2521 ggccttgctc ggcggcccgg cggtgagggc ggaggaccag agggaaggac tcgcgggaga
     2581 tcccgcggcg ctggaggtag ccgcggtaga aggcgagcga gcgatggatc ggctcggcca
     2641 gcgcctctcc cacggcgcgg ccgcgttcct cgggcccacc ggatactcgg accaccggga
     2701 tcatgcggcc tccgcgtagg ccaggaggaa ccccaccgtc cccaggatgt gctcgcggag
     2761 gccgtccacc gtggcgaact cgtccgaggc gtgcgcgccg ccgctgaacc cgaggccgcc
     2821 cgaggcccac ggcatctcga gcaggcggtc gaagatgtag aagggcgcgc accagggggc
     2881 catcgggtag gggagcggac ggcggcccag ccgatcgtag gaggcgcgca tcgcgcggga
     2941 ggcgcgggag tcgggcgcgg acctggccca ggggtagcgc tccagcggga ggacctccac
     3001 atgggggaag ccctcgcggt cgaggtggcg gcggacggcc gcggcggcct cgtcggggtc
     3061 caggtcgggg actaggcgga ggtcgagcac ggcccgggcc tcgctgggca tgatcgtgtg
     3121 gccggggccc gtgtagcccg cctcgagccc gtcgatgttg gcggtggggc ggtagatcag
     3181 ggcctcgagg agctcctcgg gggtcccctc gatcttgtag cgggaggccc tcgcgtcgcc
     3241 gaggtgctcg gccgggtcga agccggcggc gagctcgcgg aggagggggc ggtcgtcgtc
     3301 cgagggcggc gcggcggcgg ccgtgatctc gtggacgagg agctcttcgc ccggcccgac
     3361 gatcgtggcc agcgcgtgga ccaggctcca gacgggtgag gcgatccacg ccgcctcgct
     3421 ggagtggagc ggcttggcgg gcccgcccca gtccccggcc cggcagacga gctccatgct
     3481 gaggagcccc ttgcagcccg tgatgagctg ggccgagccg tcccgctcgc cgcagaggtc
     3541 gaggtcggcc ccgcagtcgg ctcggaggtc gtcggcgcgc tcggtgacga cggcgggaag
     3601 gttcatgctg cccagctcct cctcgccctc gatcacgaag cggatcgcgc aggggatccc
     3661 gcccgcgtcg cggagggcgt cgagcgcgca gaagaacgag gcgaggctgc ccttggagtt
     3721 ggccacgcct cggcagacga cggaggggcc gatgccgtcc aggtccacga tctcggcgga
     3781 gaacgggggg acggtccagt cgggctcctc ggccggctgg acatcgtaca tgccgtagcg
     3841 caggagagag ggggctcccg gcggcccggg gatctcgccc aggatcacgg ggaagcccgg
     3901 ggtctcgacg agctcggcct cgcccccggc ggcctggatc atcccgagga cggcgtcggc
     3961 gacgtcgcgg accccctcgc cggtggcgct gaccgagggc atgcgacaga ggtcgcggat
     4021 gcgctccagg taggtgtcga agcgctcgcc gacgatcgcc gcggcctggt caccccccac
     4081 agcctgctgc gtaccgacct cccttgttga tggctgatta tgaacggctc tcgcccggag
     4141 ggagcgacgg aacggcgcat cggtcgaagg cggaccctcc ggaacggcaa cccgccatcg
     4201 ggcagcggtt ctcccgccct cgacccccaa agcgtcgaga gcgggagaac ctggtcccgg
     4261 aacgctcgcg ccccccgcgc tcgcgtccca tctcccagat acgctcgttc cggttcctcg
     4321 gattggggaa gttcagaggg cggcggcgat cgcgccggct acgcgggcgt tctcctcgag
     4381 gagggccagg ttggcctcca ggctgcggcc gtccgtctcc tgggccaggc agcggagcag
     4441 gtacggcgtg aggtccttcc cgttgacggc ctcgcgcgcg gcgcgctcct cgcatccggc
     4501 gacggcgtcg gcgatctcgt cagggtcgag cgcgtagagc tccgggatcg gcacgcacac
     4561 gaggagggcg gcccccagac ccagatcccg gcgtgcacgg gcgatctccg cggcctccct
     4621 gggagagtcg gcacggtgct cgagctcgat cggggcctcg cgtgccagga agaaggggag
     4681 tcggtcggtc ccgtagccca ccacgccgac ccccagctcc tcgagtcgct ccagcgtcgc
     4741 cgccggatcg gtgatcgact tcgggccggc gcagaccagg gtgacggggg agcgggccag
     4801 ctccgcgagg tcggccgaga cgtcgccgcc tccgtggtgg acgccgccga tcccgccggt
     4861 ggcggcgacc tcgatcccgg catgggcggc gatccagagc gtggccgaga cggtggttgc
     4921 gccgaggccg cggcccgcca cggccatggg gaggtcgcgc cgggcgacct tggccgcggc
     4981 ctcggcgcac agccggtcga gctcgtcgcc ggtcgccccg acccgcacct caccgtcgtc
     5041 aacccacgtc cacgcgggga gagcgcccgc cgcgcgcacg gcctccccca tccgatcggc
     5101 ggcctcgcgg ttcctcggct cggggaggcc ctgcgccagc acgctcgtct ccagacccac
     5161 gaggggccgc tctgcgccgc ggagctcggg ggagcgggtc ggctcgctca cggccaggcc
     5221 tcctcccgcg atgccaccca tccgcccgtc gtgatggcgt ggtccagcgc acggccgagc
     5281 gatcctccgc ggaggagcgt cgccaggaag gcggcgtcga acgcgtcccc ggcgccggtg
     5341 gggtccgcga cctcgacctg cttggcgggt tcgagaagcg tcagctcggc cgaggccagc
     5401 gcggcgccgc gatccccgag cttcaccacc gccacctcgt accgttcccc gagcttcgcc
     5461 gccgcctcct cgccctcggc gtccgtcagc acctccgcct cgcgctcgtt ggccagtagc
     5521 acggtggcgg ccgaggtggc ctccaggaac cgctccgcgc cgtaggcctc gagcaggggc
     5581 caggtcgagg tctcgaccgc gaggtgccgg gcgcgggcct gctccaatgc ggccagaccg
     5641 gccgcctcgg agccctggtc gaagagcaag tagccggaga ccagcaccgc gtcggcctcg
     5701 atctcctcgg ggagatcggc gggcgagagc cgcgccgctg cgcctcgatc ggccaccatc
     5761 gatcggtccc ctccgtgcac cagcaccagc agggtcccgg tcggcgcccc cggatccacg
     5821 accagcgccg tctccacgcc gcgagcctcg agcgactcgc ggaccaggcg gcccgccgcg
     5881 tcgtcgccga cccgggcgtg gagccggacc cgcagcccct cgctcgccgc ccatgcggcg
     5941 acgttcgccc cctggccacc gggtcgcgcc cgaaccgtgc cccggacgtc gcccccctcg
     6001 agcaggggcg gcgcgtgggc cgcgatatcg agcagggcgt cccccacaac ggccaggtcc
     6061 gtcacggctc cgaggggtcg gggccgaggt cgctcgtggg gagccaggcg atgtcgggag
     6121 ggaacacgac gtccagcgtc cagtcgagcg ccacgcgggc cttgcggagc agcgtgggga
     6181 ggcgcatcag gtagtagctc cgccagagga accaggccgg cacgccggtc accggcaccc
     6241 cgaagaccag tcccacggcg ttgcggtggc ccagcgagac gagctcgccc agggtccggt
     6301 agcggaacgg aggggcggat cggccctcca gcacgtccag aagatggcgt ccgaggtaga
     6361 cgccctgccg cagggcgaac tgggcggtgg ggggcgagat ccccccgcgg ccgtcgggga
     6421 ccgcggccag gtcgccgccg gcgaacacgc cgggcgcggc ccgcaggtgc ccgtccacga
     6481 cgagcctgcc gccgggcccc gcggggagcc cgagcgtctc cgcgagggga ttgggccgca
     6541 cgccggcggc ccagatcacg gtgccggcct cgatctcccg cccgtcctcg agcgagatgc
     6601 cgccctcgtg gaccttcgcc accttcgctc cgaggacggg ctcgatgccg cgctcccgga
     6661 tcccgcgctc ggcctcggcg ctcagccgct cggggagcat cgcggtcacc cgctcgaggg
     6721 cgtccacgag ggtgacgtga ggccgcggcg cggttcggta gaaccgcgcc gcggcctccg
     6781 agagcatgtc cgccagcgcg gtggccagct ccgcgcccga gtaccctgcg cccacgaccg
     6841 ccaccctcgt caaacgcggg tcgtgcgact cggcggccgc ctccagcgag gccagcatcc
     6901 ggttccggat gcgcagggcg tcggagatcg tcttgaacgg cagggcgtgc tcgtccaccc
     6961 cctcgatccc gaaggtggcc tggaccgccc ccagccccag cacgatcgcg tcggccccgt
     7021 accgcacgac cccctcgcgc agcttcggcc gcaccgtgac ggtcttggac gcgaggtcga
     7081 tctcctcgat gtcgccgatc agcagccggg cgtggggggc gagctggcgg atcgggctga
     7141 cgacatggcg cgggtcgagg tccccggccg ccacctcggc cagcatcggc gtgaagagca
     7201 ggaagttgtc gcggctgacg atgagcacgt cggcccgcgc gcccagcccg gccgcggcca
     7261 tcacgcccgc gtacccgccg cccagcacga gcaccttcgg cctcgccatt cctcgcatcg
     7321 tagtgcccgg cggaccgacc cgaccggtcg ccctatgctg ggggtcgtgg cacgcatcct
     7381 cctgatccgg cacgcggtgg ccgaccacac ggggaagcgc ctctacgggc gctcccaggg
     7441 cgtcgcgctc tccgagcggg gccgggccga cgccgagggg ctggccgagc gcctccgccg
     7501 ggtgcggatc cacggcctct acgtcagccc gatgcagcgg tgccgggaga cggccgagac
     7561 gatcgcccgc ggccgccggc tggagccgga gatcctcgag gatctcaacg aggtcgacta
     7621 cggcgactgg acggggagga cgttcgcctc gctccgccgg acgaaggtgt ggcggcaggt
     7681 ccggacgagg ccctcgacgg cgcggttccc gaacggggag agcctcctcg aggtccagga
     7741 gcgggccgtc cgggcgatcg aggggatcgc ccgccgccac cggaggggcg tggcggccgt
     7801 cgtgacccac ggcgacgtga tccgcctcgc gctcgcgcac tacgcgggcc tccacctcga
     7861 cctgttccag cggctggagg cgtccaccgc ctcggtcacc gcgctcgcgc tgggggacgg
     7921 catcccgcgc gtcctccgcg ccaacgacac cgggcgtctg gaggacctcg tcccggggaa
     7981 ggtgggagga taggcgcgtg gagcttgacc ccgtggaccg catcaccgtc ggggcggtcg
     8041 gggagcccgg cgagcgcacc ttctacctcc aggcccgccg cgggaccgac ctcgtgacga
     8101 tcgtcgtgga gaagcagcag gtgcagctgc tgtcggcgac cgtcgtggac atgctctccc
     8161 ggctcgggac cgacgtggag gtcgaggtgg gcgagcacga gatggagctc gagcagcccc
     8221 tcgtgcccga gtggcgggcc gcgcgcatcg ccgtgggcta cgcggaggag cgtgacctct
     8281 tcatgctcga ggccgaggag ctcgaggagg aggacgagga ggccgagctg ggcgggcagg
     8341 cccggttctg ggccacccgc gagcagatgc tggcgctggc ccgccacggg atcgccgtgg
     8401 ccgcggccgg gaggcccacc tgcgagttct gcggcaaccc gatggacccc gagggccacg
     8461 tctgcccggc gatgaacggc caaggtcggc tgggggaggc gtgaccaccg ccctggccgg
     8521 cggcgagctc gaggtgctgg ggctcctgcc gggcgcctcg aatcacacct tcctgggccg
     8581 gatcggaggg ctccacgtcg tctacaagcc ggtccggggc gaggcgccgc tgtgggactt
     8641 ccccatcggg acgctggcgg cgcgcgaggt ggccgcggcg cggctggccg aggccctggg
     8701 atggcccgat gtgccgccca cggtcatgcg ggacggcccg gccgggcccg gcgccgtcca
     8761 ggagtacgtg atggcggttg agggcgagca ctacttcacg ctccgcgagg agcgggccgg
     8821 cgagctgaag gcggtggcgg cgttcgacgt ggtggccaac aacgcggatc ggaagggcgg
     8881 ccacgtcctt ctgggcgagg acgggcggct ctggtgcatc gaccacgggc tgacgttcca
     8941 cgtcgagccg aagctgcgga cggtgatctg ggacttcgcc ggcgaggccc tgcccggagg
     9001 gctggcggag gacctggagc gggtcgtggg cgagcttcgg ggcgggccgc ttcgggaggg
     9061 gctggagggg gtgctcgacc cggccgaggt gggggcggcc ggggcccggg cggaggccct
     9121 gctggaggcc ggccggttcc ccggcccggg ccccggccgc cccatcccct ggccgctcgt
     9181 ctgatgggcg ccctggagcg gtggcgcgag gcgctggcct cgtgggccat ccccgacgag
     9241 atcttggcga aggcgcccga gccgccctgg gggttccggc ccgagatctt cgccgaccga
     9301 gcccgccggg cacccgaaga gcccgagacg ccgtcgcgac gccgcgccgg cgccgccctt
     9361 cggggcggcg ggaccgtcct cgacgtgggg gccggcgccg gcgcggcgtc gctccccctc
     9421 gccccgccag ccgacgcgat cgtggccgtc gacccctccc gtgagctgct gacggccttc
     9481 gaggagctgg ccgtcgagcg ggaggtgacg ctgacgacga tcgagcggtc ctggcccgag
     9541 gcggccggcg agtcgcccgt ctgcgacgtc gtcgtctgcc accacgtcct ctacaacgtc
     9601 cccgacctcg gcccgttcgt ccgcgcgctc acggaccacg cgcgtcggct ggtcgtcgtg
     9661 gagctcaccg cccagcaccc ggtgtcctgg atggccgacc tgtggcgccg cttccacgac
     9721 ctggagcgcc ccaccgagcc caccgccgac gtcgccgtgg ccgccctgcg cgaggccggg
     9781 atcgataccg ggcgcgagga tcacctggtc ccgcgcgagg gcggcggctt tccagaccga
     9841 gaggccgccg tggcccaggc ccgccggatg ctgtgcctga cccccgatcg cgatcccgag
     9901 gtggccgagg cgctgggcga ccgcctccgc gaggggccga ccgggtgggg gatcgggccg
     9961 cccgcggccc gcgtcgtcac cctatggtgg gagccgtgac ctccgggcga tccgagagcc
    10021 ggtagacgaa ccggacggcc gtccaggtct cgtcgatcga ggcggtcttg ttgtccacga
    10081 gccccgtggc caggcccgct tcctgcacca cggtgaagtc gaggtcgctg gggacgccgg
    10141 aggccttctt cgggtaggcg atccacaggc tgcccgtgta gtccagcgcc ttccggagcg
    10201 aggggagccg ccgctcaagc gccgagcgcc ggtgggcgaa cagcaccacg acgtcgtgcg
    10261 gccctccgac ccgtgtggcg atggtcaccc ctcgaggtag cggcgccagc gccccctcga
    10321 atccgtcggg ctgcccgacg aagacgacgc gcgacccggc cgtgatcccc agcttcctgg
    10381 ccagcggcgt gctcacgagg cccgcctggc cacgtagtct cggagcacga agtcgcccac
    10441 cgactcgccc gacatcagga agatgcggcc cgaggtcagc ctcgccaggc gctccatgaa
    10501 ccgcgccagg cccggagagt cctcgagcat gaacacgttg agcgtcacgc cggcccgggc
    10561 cagccgcatc gcctccgaca gcgtccgccg gatcgtctcc cgcaccggcg gccacgagaa
    10621 gaagacctcg tcgccctcca ggtgcgcggt cggctcgccg tcggtgacca tgatcacctg
    10681 gcgcgtggcc cgcgggtgtc gggccagcag ccgccccgcc agatggaag
//
LOCUS       DASUTZ010000028         2317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_525569, whole genome shotgun sequence.
ACCESSION   DASUTZ010000028 DASUTZ010000000
VERSION     DASUTZ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525569"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VF097_01280"
     CDS             complement(<1..873)
                     /locus_tag="VF097_01280"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6261458.1"
                     /translation="MEPQGLDVDLGGYRLHLDCRGEGSPAVILEAAIWDIGLTWARVQ
                     PQVARFTRVCSYDRAGLGRSERGPRPRTVHVMVEELRALLGAAGVPPPYVLVAHSFGA
                     LIAKLFAHRHPSEVAGIVLVDGAHEDQLARFPRTVRAAWEPMSEMQLGALRAAREAIA
                     AGTFDPTMAPVPDLPPDAAALYRSLLTSGPGMVDTEIEEHEALEESQRIVREAGVSSL
                     GDLPLVALSHGVPPPPFPPELGVTPDDQERYEDVWQDLQGELAALSSRGERRVAEGAS
                     HMIHHDRPDLVVEAI"
     gene            complement(879..1580)
                     /locus_tag="VF097_01285"
     CDS             complement(879..1580)
                     /locus_tag="VF097_01285"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX6261459.1"
                     /translation="MGATRTTAFVLYPGLTPLDLVGPLQVMSVLERFAPECRAIVVGE
                     RVEPMETDAGISIVPQATFEEVPEPRVVVVPGGGSPTIRQMGNEVVLDYLRRVADRAE
                     VVASVCTGALLLAAAGLLEGRRATTHWGYHALLERLGATYVPERWVDDPPFLTSAGVS
                     AGIDMGLELAARVADVDRARLVQLGIEYDPRPPFGPIDWSTVDRDVLTPLAESSVRTE
                     LADRPHLQRRLLPEG"
     gene            complement(1582..>2317)
                     /locus_tag="VF097_01290"
     CDS             complement(1582..>2317)
                     /locus_tag="VF097_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6261460.1"
                     /translation="DDGVFVREDEAEAKGLEVGETLRMEFGAAGVVPMEVRGIYREGW
                     LAGTYLISLQAYEANYTEQLDNLLFVKTAPTLSGREARQAIAALQEAFPGVEIQDQAE
                     LRRSQEQQVNSLLGMVTALLGLAILIALLGIVNTLALSVLERTRELGLLRAVGMSRRQ
                     VRRMIRWESVIIALIGGVLGLAVGLLFGWALVQALADEGITSFTVPFGQLAIFLALAG
                     VAGVLAAIGPARRAARLDVLQAIAYE"
BASE COUNT          340 a          861 c          804 g          312 t
ORIGIN      
        1 gatcgcctcg accacgaggt cggggcggtc gtggtggatc atgtggctcg cgccctcggc
       61 cacccggcgc tccccgcgtg acgagagggc ggccagctcg ccctgcaagt cctgccagac
      121 gtcctcgtat cgctcttggt cgtcgggggt gacgccgagc tcgggcggga acggcggcgg
      181 cggcaccccg tgcgagagcg ccaccaacgg gagatccccg agcgaggaga cacccgcctc
      241 gcggacgatc cgctgggatt cctccagcgc ctcgtgctcc tcgatctcgg tgtcgaccat
      301 ccccggtccc gaggtgagca gcgaccgata gagggcggcg gcgtcgggag gcaggtcggg
      361 gaccggcgcc atcgtcgggt cgaacgtgcc cgcggcgatc gcctccctcg ccgcgcgcag
      421 ggccccgagc tgcatctcgg acatcggttc ccacgcggcc cgcaccgtcc gggggaagcg
      481 ggccagctgg tcctcgtgcg cgccgtccac gaggacgata cccgcgacct cgctcgggtg
      541 ccggtgggcg aagagcttcg cgatcagcgc cccgaacgag tgggccacga ggacgtacgg
      601 gggcggcacg ccggccgccc caagcagggc acggagctcc tcgaccatga cgtgcacggt
      661 gcgcgggcgg gggcctcgct cgctccggcc gaggccggcg cggtcgtacg agcagaccct
      721 cgtgaagcgc gccacctggg gctgcaccct ggcccaggtc agcccgatgt cccagatcgc
      781 cgcctcgagg atcaccgctg gcgatccttc gccccggcag tccaggtgga ggcgatagcc
      841 tccgaggtcg acgtccaggc cctgtggctc cacggtgctc agccttcggg gaggagccgt
      901 cgctggaggt gggggcggtc ggcgagttcg gttcgcaccg atgactccgc gaggggggtg
      961 aggacgtccc gatcgacggt cgaccagtcg atcggaccga acggcggccg ggggtcgtac
     1021 tcgatcccca gctggaccag cctggcccgg tccacgtccg cgaccctggc ggcgagctcc
     1081 aggcccatgt cgatccctgc cgagacgccg gcgctcgtca agaagggcgg gtcgtcgacc
     1141 cagcgctcgg ggacgtacgt ggcgccgagc cgctccagca gggcgtggta cccccagtgc
     1201 gtcgtggccc gccggccctc gaggagaccg gccgccgcca gcagcagcgc gccggtgcac
     1261 acggaggcca cgacctcggc ccggtccgcc accctgcgca ggtagtcgag cacaacctcg
     1321 ttccccatct ggcggatcgt ggggcttcca ccgcccggaa cgacgaccac ccggggctcg
     1381 gggacctcct cgaacgtggc ctggggcacg atcgagatgc cggcgtccgt ctccatcggc
     1441 tccacccttt cgccgaccac gatcgccctg cactcggggg cgaagcgctc cagcacgctc
     1501 atcacctgga gcggccccac gaggtcgagc ggcgtgagtc ctgggtagag gacgaaggcg
     1561 gtggtccggg tggctcccat gtcactcgta ggcgatggcc tgcagcacgt cgagtcgggc
     1621 cgcccgtctg gccggcccga tcgcggcgag cacgcccgcc actcccgcca gcgccaggaa
     1681 gatcgccagc tggccgaacg ggaccgtgaa cgacgtgatg ccctcgtcgg ccagagcctg
     1741 gaccagcgcc cacccgaaca acaggccgac ggccagcccc agcacgccgc cgatcagcgc
     1801 gatgatgacc gactcccacc ggatcatccg gcgaacctgc cgcctgctca ttcccaccgc
     1861 tcgaagcagg cccagctccc gcgtccgctc cagcaccgag agcgcgagcg tgttgacgat
     1921 gcccagcagg gcgatgagga tcgccagccc gagcagtgcc gtgaccatcc cgaggaggct
     1981 gttcacctgc tgctcctggg accgacgcag ctccgcctgg tcctggatct cgactcccgg
     2041 gaacgcctcc tggagcgcgg cgatcgcctg cctcgcctca cggcccgaga gcgtgggggc
     2101 cgtcttgacg aacagcaggt tgtccagctg ctcggtgtag ttggcctcgt acgcctggag
     2161 cgagatcagg taggtgccgg ccagccagcc ctcccggtag atccctcgaa cctccatggg
     2221 gacgacgccg gcggccccga actccatgcg cagggtttcg cctacctcga gccccttcgc
     2281 ctcagcctcg tcctctcgga cgaagacgcc gtcgtca
//
LOCUS       DASUTZ010000029         6131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_266706, whole genome shotgun sequence.
ACCESSION   DASUTZ010000029 DASUTZ010000000
VERSION     DASUTZ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6131
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266706"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VF097_01295"
     CDS             complement(<1..200)
                     /locus_tag="VF097_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005451143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX6261461.1"
                     /translation="MRRITPILVALVLAGLTAPAGATAETEPVRFATFNASLNRNFEG
                     QLIADLSTPANAQARTVAEIIQR"
     gene            complement(265..645)
                     /locus_tag="VF097_01300"
     CDS             complement(265..645)
                     /locus_tag="VF097_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine synthase"
                     /protein_id="HEX6261462.1"
                     /translation="MIVRTVEDVAGTPAQVSGGLWTSTRLLLRDDGLGFTMTETVIEP
                     GMDEELWYKHHLEACFCVEGEAEIEELETGERHEIRPGTLYALDRHDRHRLRTRTRVR
                     LICVFSPALRGDEIHDEDGSYLLD"
     gene            719..1453
                     /locus_tag="VF097_01305"
     CDS             719..1453
                     /locus_tag="VF097_01305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6261463.1"
                     /translation="MRTSAPARDRPRDGVRVYEDRWSSRRVVQVRPHLWRRLEPLLGG
                     ARILEIGSGLRPTAPARGSFFVERSPRAVRALRWAGGRAVQTGGWELPFKDGAFDAVL
                     ALELLEHVEEDERMLAEIVRVLRPGGTALVSVPLHMSLWSAIDDACAHVRRYEPDELL
                     EKIGAAGLRSQGYLTRPAQVRPGLARLGIGFMSRFPRLSNWSLQHVVFPVASGWARAF
                     GRVQWAEPGTPTPEGAGGMTVLATKG"
     gene            1600..1800
                     /locus_tag="VF097_01310"
     CDS             1600..1800
                     /locus_tag="VF097_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261464.1"
                     /translation="MTSKMTAVGDASLTGWRRKVGDPIARGVASRTRFSAEQVRAFLG
                     FALIAITLYSLFKRARRVTRET"
     regulatory      1841..2044
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2156..3085
                     /locus_tag="VF097_01315"
     CDS             2156..3085
                     /locus_tag="VF097_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010836764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6261465.1"
                     /translation="MRRGTGGLFLTLVLILGACGDDAEPQAGPSPSPGGGSFPVTVEA
                     ANGPVTMDERPERIVSLSPTATEMLFAIGAGDQIVAVDDQSNYPPEAPVTDLSGFEPN
                     IEAIAGYEPDLVVISNDISDLIASLEGLDIPVLLQPAAEDLQGTYEQMEQLGELTGQP
                     SGASDEVREMQAAIDELLEEVPEFVRAPTHYFELDPTFFTVTSDTFVGQVLAMIGMQS
                     IADEAEGASSGYPQLSSEFIVAANPDFIFLADTKCCGESAESVAARPGWDGIQAVQEG
                     NVVALDDDVASRWGPRVVDLLEVVVEAVAGAGS"
     gene            3085..4143
                     /locus_tag="VF097_01320"
     CDS             3085..4143
                     /locus_tag="VF097_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6261466.1"
                     /translation="MATATAEVRLRPTRLRPGLLGIALGVLALAGLAGVLIGPVGLSP
                     KGVLLELLDRVPFVGVSSGLAEREQAILWQIRLPRVVVGGMVGAALAMAGAAYQGVFR
                     NPLADPYLLGAAAGAGLGATLAVAYLPSAGGPVGPLPAAAFLGALVGVGAAYGLGRSV
                     GRSRTSGTLILAGVAVASFLTAVQTYFQQREDDTLRQVFAWILGTLGTVTWREAALMA
                     PYFLVGGVVLLVHRRLLDVLSLGDEEAESLGVRAGRVRLVIVVAASLATAAAVAVSGL
                     IGFVGIIVPHAVRLAFGGSYRLVLPLSIAFGAAFLVLTDLAARTILAPAEIPIGVVTA
                     FFGAPFFLAVLRRSKGMA"
     gene            4140..4952
                     /locus_tag="VF097_01325"
     CDS             4140..4952
                     /locus_tag="VF097_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6261467.1"
                     /translation="MIGLGLRAVTAAYDGTPALRGIDLEVAPGSWTGLIGPNGSGKTT
                     LLRAVAGLMPHGGTVLLGGEEVARMPRRAAARLVAYVPQRPVVPEPMPVIDYVLTGRT
                     PYISYLGSEGVSDLEVAARVLARLELEDLAERPLGTLSGGELQRAIIGRALAQGAPLL
                     LLDEPTSALDVGHQQQALELIDALRREEGLTVVAAMHDLTLAGQFAERLILLDRGTVA
                     AEGPVREVLTESTIGRHYGARVRILDDDGGGVVVIPVRDAAAAPAGEREKPA"
     gene            4949..5500
                     /gene="cobU"
                     /locus_tag="VF097_01330"
     CDS             4949..5500
                     /gene="cobU"
                     /locus_tag="VF097_01330"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019009758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HEX6261468.1"
                     /translation="MSLTLLIGGARAGKSDLAVALAERAGKPVSFVATARAIDEEMAD
                     RIERHRAERPSGWVTVEAPVDLRGALGRVPEGATAIVDCLTLWVSNLLEDGCDDEEVV
                     AVAEAAAEAARARDGRTIAVTNEVGAGIVPMHPLSRRYRDLLGRVNAIWASRASRALL
                     VVAGRVLPLEEGWAPLDEEAGRG"
     gene            5493..>6131
                     /locus_tag="VF097_01335"
     CDS             5493..>6131
                     /locus_tag="VF097_01335"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF014347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEX6261469.1"
                     /translation="MDDPIRELERALAGLPGPDEAARRAVADRAVRVLRPRGALAGLD
                     ALAVWLAGWRGAPPAVEAPACVVFAADHGVAAEGVSAYPPEVTRSMLAALKEGVATAP
                     VLADQVGAAFSVADVGVGRPTGNLLVEPAMGQERFGEAIAAGRRAVAEADGCDLLVLG
                     EMGIGNTTAAAAVAATLFGGPVEQWTGRGTGVDDETYARKVAVVDVARRRVGD"
BASE COUNT          753 a         2007 c         2462 g          909 t
ORIGIN      
        1 cgctggatga tctcggcgac cgtgcgcgcc tgggcgttgg ccggcgtgga caggtcggcg
       61 atcagctgtc cctcgaagtt ccggttcagc gaggcgttga aggtggcgaa ccggacgggc
      121 tccgtctctg cggtcgcccc cgccggggcg gtgagtccgg cgagcacgag cgccaccagg
      181 atcggcgtga tgcgtcgcat gagctctccc ttccgtttgg cggcgccatg ctatcccgag
      241 cgggtccgaa gctcacatcc gagctcagtc caggaggtac gagccgtcct cgtcgtggat
      301 ctcatccccg cggagggccg ggctgaacac acagatcagt cggaccctag tccgcgtccg
      361 cagccggtgg cgatcgtggc ggtcgagggc gtagagcgtc cccggacgga tctcgtggcg
      421 ctcacccgtc tccagctcct cgatctcggc ctcgccctcc acgcagaagc aggcctcgag
      481 gtgatgcttg taccagagct cctcgtccat gccgggctcg atcaccgtct cggtcatcgt
      541 gaagcccagc ccgtcgtcgc gcagcagcag cctcgtgctg gtccacaggc cgcccgagac
      601 ctgggccggc gtccccgcca cgtcctcgac cgtccgaacg atcatcgtcg cctccttcgt
      661 ccgcggaagc ctagcgcggc gtgggtagca ccggagcgaa cgggtatctt gttggccgat
      721 gagaacgtca gccccggccc gggaccggcc tcgcgacggc gtccgcgtgt acgaggatcg
      781 atggagctcg cgtcgggttg tccaggtgcg gccgcacctc tggaggcgcc tcgagccgct
      841 gctcggcggg gcccggatcc tcgagatcgg ctcggggctc aggccgacgg cgcccgcccg
      901 cggctccttc ttcgtggagc ggtcgccgcg ggcggtccgg gcgctgcgat gggccggggg
      961 gcgggcggtg cagacggggg ggtgggagct cccgttcaag gatggagcct tcgacgcggt
     1021 cttggccctc gagctcctcg agcacgtgga ggaggacgag cggatgctgg ccgagatcgt
     1081 ccgggtcctg cgccccgggg gaacggctct ggtctcggtc ccgctccaca tgtcgctctg
     1141 gtcggcgatc gacgacgcgt gcgcgcacgt ccggcgatac gagcccgatg agctgttgga
     1201 gaagatcggc gcggcaggcc tgcgctccca gggctacctc acccgtcccg cgcaggtgcg
     1261 cccgggcctg gcgcgcctcg ggatcggctt catgtcgcgg ttccccaggc tctcgaactg
     1321 gtcgctccag cacgtggtgt tcccggtcgc ttcggggtgg gccagggcct tcggaagggt
     1381 tcagtgggcc gagcccggga cgccgacgcc agagggcgcc gggggcatga ccgtgctggc
     1441 cacgaagggg tagcgacaaa cggaaccgcg agagtccgaa gactcccgcg gttccaaggc
     1501 gacttgtaca ccgccgcggc cctccggcgc aacccgcgcc ccggcttttg tcactctttc
     1561 tttgggttcg agggggccgg ggcagggaag aacgacgcca tgacctccaa gatgaccgcg
     1621 gtgggcgacg cctcgctcac gggatggcga cggaaggtgg gcgacccgat cgcgcgcggc
     1681 gtggcgtcga ggacccggtt ctcggccgag caggtccgcg cgttcctcgg gttcgcgctg
     1741 atcgccataa ccctgtactc gctgttcaag cgggcgcgcc gcgtcacccg agaaacctga
     1801 cgccgctcgg cttgtctccg gccccgctcc tgcgggagaa tcggagctga cggtccgagg
     1861 tggcgacggc ggggacggct cccccgccgg agctgaaaag ggaagccggt gagaatccgg
     1921 cacggtcgcg ccactgtgac cggggagcga ccccgaggga tgccactggg gctcaggtcc
     1981 tgggaaggct cggggaagtg aggatccgga agtcaggaga cctgcctcgg aagggagcac
     2041 cgtccctcgc gtgccggggg cggcccacgc ggcccccgct gagggggcct gcttcgttcc
     2101 cgggcctcga gggacggccg acgcggccgc gctcgacggc cgggaaggag gcgggatgcg
     2161 ccgcgggacc ggaggcctgt tcctcaccct cgtgctgatc ctcggagcgt gtggcgacga
     2221 cgccgagcca caggcgggac cctcgccctc gcctgggggc ggttcgttcc ccgtgacggt
     2281 cgaggccgcc aacgggcccg tgacgatgga tgagcggccc gagcggatcg tctcgctctc
     2341 tcccacggcc acggagatgc tgttcgccat cggagccggc gatcagatcg tcgccgtgga
     2401 cgaccagtcg aactaccccc ccgaggctcc ggtgaccgac ctctccgggt tcgagcccaa
     2461 catcgaggcg atcgccgggt acgagccaga cctcgtggtg atctcgaacg acatcagcga
     2521 cctcatcgcc tcgctggagg ggctggacat cccggtgctg ctgcagccgg cggccgagga
     2581 tctccaggga acgtacgagc agatggagca gctgggcgag ctgaccgggc agccctcggg
     2641 ggcgagcgac gaggtgaggg agatgcaggc cgcgatcgac gagctgctcg aggaggttcc
     2701 ggagttcgta cgggcgccga cccactactt cgagctcgac ccgaccttct tcacggtgac
     2761 gtcggacacg ttcgtggggc aggtgctggc gatgatcggg atgcagagca tcgccgacga
     2821 ggccgagggg gcgagcagcg gataccccca gctcagctcg gagttcatcg tcgcggccaa
     2881 cccggacttc atcttcctgg cggacacgaa gtgctgcggg gagtcggcgg agtcggtcgc
     2941 ggcgcgtccc ggatgggacg gcatccaggc cgtccaggaa gggaacgtgg tggccctcga
     3001 cgacgacgtc gcctcgcggt gggggccgcg cgtggtggac ctcctcgagg tcgtggtcga
     3061 ggccgtggcc ggggcagggt cctgatggcg acggcgaccg cggaggtgag gctccggccc
     3121 acccgcctgc ggcccgggct gctgggcatc gcgctggggg tgctggcact ggctgggctc
     3181 gccggtgtgc tgatcgggcc ggtggggctc tcgccgaagg gcgtcctcct ggagctgctg
     3241 gatcgggtcc ccttcgtggg ggtgagctcg gggctcgctg agcgggagca ggcgatcctg
     3301 tggcagatca ggctccctcg ggtcgtggtc ggggggatgg tgggggcggc cctggcaatg
     3361 gccggcgcgg cgtaccaggg cgtgttccgg aacccgctgg ccgaccccta cctgctcggg
     3421 gccgccgccg gggcgggcct cggggccacg ctcgccgtgg cctacctgcc gtcggctggg
     3481 ggccccgtcg gcccgctccc ggcggcggcc ttcctcggcg cgctcgtggg cgtgggcgcc
     3541 gcctacgggc tgggccgctc ggtgggccgg tcccggacga gcgggaccct gatcctggcg
     3601 ggcgtggccg tggcctcgtt cctgaccgcg gtgcagacct acttccagca gcgcgaggac
     3661 gacacgctgc gccaggtgtt cgcttggatc ctggggacgc tgggcaccgt gacctggcgc
     3721 gaggcggccc tgatggctcc ctacttcctg gtggggggcg tcgtgctact ggtgcaccgg
     3781 cgcctgctgg acgtgctgtc gctcggcgac gaggaggccg agagcctggg ggtccgggcc
     3841 gggcgggtgc ggctcgtgat cgtggtcgcg gccagcctgg ccacggcggc ggcggtggcg
     3901 gtcagcgggc tgatcgggtt cgtcgggatc atcgtccccc acgcggtgcg tctggcgttc
     3961 gggggctcgt accggctggt gcttcccctg tccatcgcgt tcggcgccgc cttcctcgtg
     4021 ctgaccgatc tcgccgcgcg caccatcctg gccccggccg agatacccat cggcgtcgtg
     4081 accgccttct tcggagcgcc gttcttcctc gcggtgcttc gccgctcgaa ggggatggcg
     4141 tgatcggcct cgggctacgg gcggtgacgg ccgcctacga cgggacgccg gcgctccggg
     4201 gtatcgacct cgaggtcgct ccggggtcgt ggaccgggct gatcgggccg aacggctcgg
     4261 ggaagaccac gctcctccgc gcggtggccg gcctgatgcc gcatggggga acggtgctcc
     4321 tggggggcga ggaggttgcg cggatgcctc gccgcgcggc cgcgcgcctc gtggcctacg
     4381 tgccccagcg ccccgtcgtg cccgagccga tgccggtgat cgactacgtg ctcacggggc
     4441 ggacgccgta catctcgtac ctggggagcg agggggtctc cgacctggag gtggcggctc
     4501 gggtgcttgc tcggctcgag ctcgaggacc tagcggagcg gcccctgggc acgctctcgg
     4561 gaggagagct ccagcgggcg atcatcgggc gggcgctggc gcagggcgcg ccgctgctgc
     4621 tgctcgacga gcccacgagc gcgctggacg tggggcacca gcagcaggcc ctcgagctga
     4681 tcgacgctct gcggagggag gaggggctca cggtcgtggc tgcgatgcac gacctgacgc
     4741 tggccgggca gttcgcggag cggctcatcc ttctcgatcg cgggacggtt gccgctgagg
     4801 ggcccgttcg ggaggtcctg accgagtcga cgatcgggcg gcactacggg gcccgggtcc
     4861 ggatcctgga cgacgacggc gggggcgtgg tggtgatccc cgtgagggac gcggccgctg
     4921 cgccggccgg tgagcgggaa aagccggcgt gagcctgacg ctgctgatcg gcggggcccg
     4981 cgccgggaag tcggacctgg ccgtggcgct ggcggagcgg gccgggaagc cggtgagctt
     5041 cgtggccacg gcccgggcga tcgacgagga gatggcggac cggatcgagc ggcaccgggc
     5101 cgagcggccg tccgggtggg tgacggtcga ggcgccggtg gatctccggg gagccctggg
     5161 gagggtgccg gagggcgcga cggcgatcgt agactgcctg acgctctggg tctcgaacct
     5221 cctcgaggac gggtgcgacg acgaggaggt ggtggccgta gccgaggccg cggcggaggc
     5281 ggcccgggcc cgggacggcc ggacgatcgc ggtgaccaac gaggtcgggg ccgggatcgt
     5341 ccccatgcat cctctctccc gccggtatcg ggatctgttg gggcgggtga acgcgatctg
     5401 ggcgtcgcgg gcgtcgcggg cgctgctcgt ggtggcgggt cgtgtgctgc ccctcgagga
     5461 ggggtgggcg cccctggatg aggaggccgg ccgtggatga cccgatccgc gagctggagc
     5521 gagcgctggc gggcctcccg ggtccggacg aggcggcacg acgggcggtg gcggacaggg
     5581 cggtccgggt cctcaggccg aggggggcgc ttgccgggct ggacgcgttg gccgtgtggc
     5641 tggccgggtg gcggggcgcg cctccggcgg tggaagcgcc ggcgtgcgtc gtgttcgccg
     5701 ccgaccacgg cgtggcagcc gagggggtga gcgcgtatcc gccagaggtg acgcgctcga
     5761 tgctggccgc gttgaaggag ggcgtcgcca cggcccccgt gctggccgac caggtggggg
     5821 cggcgttctc cgtcgcggac gtcggggtgg ggcggcccac ggggaacctg ctcgtggagc
     5881 cggcgatggg gcaggagcgg ttcggggagg cgatcgccgc cggtcggcgg gcggtcgcgg
     5941 aggcggacgg gtgcgacctg ctcgtcctcg gcgagatggg gatcgggaac acgacggcgg
     6001 ctgcggcggt cgccgccacc ctgttcggcg gcccggtgga gcagtggacg gggcgcggaa
     6061 cgggggtgga cgacgagacg tacgcgcgga aagtggcggt ggtggacgtc gcgcggcggc
     6121 gcgtcgggga c
//
LOCUS       DASUTZ010000030        15694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_525801, whole genome shotgun sequence.
ACCESSION   DASUTZ010000030 DASUTZ010000000
VERSION     DASUTZ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 15694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15694
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525801"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..562
                     /gene="infC"
                     /locus_tag="VF097_01340"
     CDS             98..562
                     /gene="infC"
                     /locus_tag="VF097_01340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX6261470.1"
                     /translation="MVGPDGGQIGIVSTRDALRQADELDLDLVEVAPQAKPPVARIMD
                     YGKYKYERDVRQKEARKRQARVEVKEIKFRPKIDTHDYETKKGHVVRFLTAGNKVKVT
                     IMFRGREMAHTELGRKILDRLVGDLEDMAQVEQMPKLDGRNMVMVISPTKKG"
     gene            574..768
                     /gene="rpmI"
                     /locus_tag="VF097_01345"
     CDS             574..768
                     /gene="rpmI"
                     /locus_tag="VF097_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013979589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX6261471.1"
                     /translation="MAKTKTHRGAAKRFRVTRSGKVLHRKANRGHLLGKKSSSRKRRL
                     ARASEVTAEKRTVLRLLGRR"
     gene            768..1127
                     /gene="rplT"
                     /locus_tag="VF097_01350"
     CDS             768..1127
                     /gene="rplT"
                     /locus_tag="VF097_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX6261472.1"
                     /translation="MPRVKRGVHGRKKRREVLAQAKGYRGARSRRYRVAKEQLLHSGV
                     YAYRDRRRRKGEFRRLWITRIGAASRQHGLSYSRFMTGLKAAEVDLDRKVLADIAATD
                     PEGFAGLVEVARRGLGE"
     gene            1151..2572
                     /locus_tag="VF097_01355"
     CDS             1151..2572
                     /locus_tag="VF097_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEX6261473.1"
                     /translation="MITSTKNPRIVAAAKLKKRAFRDREARFLVEGAQVTLEAIDAGR
                     VEVLFHTMDEHGRVDALVDRARGSGVRTEAVTQQVVEHLTSTVTPQGIVGVATYVDVP
                     LGELQPDPSLVAVLSSVRDPGNAGTILRTADAAGADGVVFGRESVDVYNAKTVRASAG
                     SLFHLPVFRDVDVVEALEGLRRGGLQVLATAADGERTIYEADLSAPTALVFGNEAHGL
                     EPEIRALADVTIRVPIRGRAESLNLAASAAVTLFEAVRAREAPDGLAQIAGLIGPAGH
                     DVRSPLTALTGFVTTLARRWDGFSDEERREIVEGMVLDGHRTAALVRLVVDAVRILSG
                     APLTSTSERADVGAAVRWLAELFAASEDFPDLEVEGEARARSDPDRIRALLFGLVDGA
                     MWWGSEGPVSLRLSEEGSYAVVEAWRTGDRHGDEEPTDVLGSPEHGGRVSLYAARLVA
                     ERLGGSLEVETDGGIRLRLRLPV"
     gene            complement(2569..3048)
                     /locus_tag="VF097_01360"
     CDS             complement(2569..3048)
                     /locus_tag="VF097_01360"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6261474.1"
                     /translation="MLGIRWSDADPSGLLIFTMEVEIPRPPEVVWPYLVDWEGLDRWM
                     EEARAFRVLGEPREGVGVEAEATISILGITTRDLVRVTRWEPPAVLEIEHLGWVSGRG
                     LMELGVTAVGSHLFWREALRPPLGPLGRIGMRLMSPVIHRTFRRDLETLRELIERET"
     gene            3094..4152
                     /gene="pheS"
                     /locus_tag="VF097_01365"
     CDS             3094..4152
                     /gene="pheS"
                     /locus_tag="VF097_01365"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008337628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX6261475.1"
                     /translation="MDPGETIAALERERERGLAAIREAASLEDLREAEVAVLGRRAPV
                     PTIQRSLGEAAPDDRPGLGKAINEVFEAFREALSDRERELAAEQERAVGEVDAVDVTL
                     PGRRLPMGALHVLTQTERAMVDAFVALGYRVMEGPEVETDWYNFEALNIPPDHPARTP
                     QDTLYLDVPGASDLLLRTQTSPMQIKAMQAQPPPLYVVVPGRTYRNDTLDATHSPVFR
                     QVEGLAVDEGLSLADMKGTLEAWARAMFSEDRRVRLRPDYFPFVEPGAQVDVTCFACD
                     GEGCRICGTGWLEIMGAGMVHPKVLENGGVDPERYTGYAWGIGVERVAMLRHGIPDIR
                     LFYEGDVRFLEQFARGWA"
     gene            4149..6524
                     /gene="pheT"
                     /locus_tag="VF097_01370"
     CDS             4149..6524
                     /gene="pheT"
                     /locus_tag="VF097_01370"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX6261476.1"
                     /translation="MRVPYSWLREIVPTRLSPEEVAVALTEHGVEVESVLRPWEALTG
                     VVVARVLEVTDHPRADRLSLATVDAGGEERRLVVGVRNMGPGDLVPYAPPGATLPGLD
                     RPLERRTIRGETSDGMLCSPKELGISADHSGILILPEGESGQDVAGLFDLDDAVIDIE
                     VFPNRPDLLSVLGVAREVAAITGDELRVPTASVLEADEKGAAVASVQVDDPKRCPRYL
                     ARVIRGVAIAPSPLRAQIRLTACGMRPVSNVVDATNYALLELGHPMHPFDLRTLSGGA
                     ILVRRATEGERLVTLDGVERAFTSEDLMIADPERAVAVAGVMGGDATEVGDATTDVLL
                     ESAYFDPLGVLRTARRLGVRTEASIRFERGADPEAVADAALHAAGLIAAWAGGAVLSG
                     EIDVGRPPPRRRVAVRPSRASMLLGMEVSAADVLDVMGRLGIDGEEREGSVEIEVPGR
                     RVDLEIEADLIEEVGRVRGYDALPSTLPGVAQAGGLDPEQRALRRTEDALVRAGLMQI
                     HSVSFASGEDLELFEDERAAGVRVANPVSEEQRFLRSSLLPGLLRAAALSIGHRRAAV
                     RLFERGHVMRLEEEVVEEDAVAALLTGPALEVWPGERRPQDVLDAKGAMEHLLEELGV
                     DGWSLGDPPGPPYHPGRSARVLLNGEPIGELGELHPSVGEGFDVPGRVAVFELRAAPL
                     VAAGAGLVELRPVSRFPPIRRDLAFVVDRSVAAADVRASLVDAAGPLLDRVLLFDVYD
                     GEPLPEGKRSLAFAVDLRAADRTLTDDEAEEVVAAIEARLASGFGAELRAG"
     gene            6526..9012
                     /gene="pepN"
                     /locus_tag="VF097_01375"
     CDS             6526..9012
                     /gene="pepN"
                     /locus_tag="VF097_01375"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEX6261477.1"
                     /translation="MKENLTRDEAAGRAALVRDVAYEIHLDLTGAERFRSQTVIRFVA
                     EPGTSTFLDCTAVEVHAAELNGESLPEGAVTEHRIALGPLAGRNEVRVVADMAYRAGE
                     HGMTRFEDPVDGAVYLFSDAEPFGAHRIYPCFDQPDVKGTFRIDVTAPASWEVASNMA
                     AEAEPEAEGGARRWSFSESPRMSTYLAAVVAGPFHVARDRHGEIDLGIYCRATLAQHL
                     DPDEILEVTKQGFDFFEKAFATPYAFGKYDQVFVPEYAAGAMENPGLVTFNEIHIFRS
                     RVTDARREGRAETILHEMAHMWFGDLVTMRWWDDLWLNETFATFASMLAMERATRFTE
                     PWTSFAHTEKPWASRQDQLPTTHPIVADVPDVESVFLNFDGITYAKGAAVMRQLVAWV
                     GLERFLEGMRVYFERHAWGNAALADFLAVHEETSGRDLGAWAEVWLRASGINALTPEV
                     GEGVAILQEGEPLRPHRLAVGAFSPEGEKLVRAGRSELDVTGARTEVPELSSDAALVV
                     LNDEDLTYAKVRFDGRSLETLREGLSRIEDGLSRAVCWRALWDMTRDGEWPAREFLAT
                     VLDHVHAETMPSAVQRILGQAGAAVSLLGDPANRDAAGSAMATAALEALRAAAAGSDL
                     QLIWARTFAAAARTPEHLDLVLALLDGEERFGGLAMDTDLRWHLVRSLASAGRADEGL
                     IAVEQERDPTDEGAREASGARAARPTAGAKAEAWEALTGPEPPRLASARAILAGFQDP
                     EQEEVLAPYARRYFDTLDDAWERFDLSLALSFARGAFPSAIVSEETVAMTDEVLARED
                     LPGPIRRVLLEGRDDLLRALRARATDAAAG"
     gene            9053..9973
                     /gene="argF"
                     /locus_tag="VF097_01380"
     CDS             9053..9973
                     /gene="argF"
                     /locus_tag="VF097_01380"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018635620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX6261478.1"
                     /translation="MARHFLSMDDLTAEELQGLLDLSRKVKASPEDHAAALGGRAVGM
                     IFEKPSTRTRVSFEVGIAMMGGHPLALSSSELQLGRGETIEDTGRVLSRYLDAVVLRT
                     FGQERLEALASTSTVPVVNALSDLEHPCQCLADLLTVLEQKGELAGQTLAYLGDGNNV
                     AHSLLLGGAKVGMHVRVATPAGYEPIPQIVERAAEIATVTGGSVQVTADPIAAATKAD
                     VLYTDVWASMGQEAEATERVLVFAPYRLDGEKVAVAAEDVVVLHCLPAHRGEEITDEV
                     IDGPHSVVWDQAENRLHAQKALLLRLLGIA"
     gene            9977..11695
                     /gene="polX"
                     /locus_tag="VF097_01385"
     CDS             9977..11695
                     /gene="polX"
                     /locus_tag="VF097_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEX6261479.1"
                     /translation="MARSNDEVAALLQEYSDLLAILGDDSFKSRAYEKAARSVEGYHA
                     EVSTLDPAGCRAIPNVGASVAEKIQEVLRTGTFQALEDLRADIPDGVRALLGIPTLGP
                     KKALKLYEELQIASPEELLDALHEQRIRELPGFGPKTEENLLRGIQMLQRSGGRVQIG
                     VAMDLAEELLGHLTEVPQVRRATFAGSLRRMAETVGDIDLLAASEDPIPVMGAFASGP
                     YVREVLAKGETKTSVLTTKGLQVDLRVVEPAVWGAALQYFTGSKAHNVHVREMAVRKG
                     LKLSEYGLFRAKSGKLIVAETEEEVYERLGLPWIPPPLREDRGEIEAALGDGLPTLIE
                     RRDVKGDLHTHTDLTDGLATLEEMVAAAERVGYRYYAITDHAELLYMQRMTQEKALAQ
                     RERIRELDERSKVTLLHGSELNIQPDGSVDWDEEFLSGFDVLVASIHSHLTMPKAEMT
                     KRMIRAMEHPCVNVIGHPTARQIGRRPPIDLDLEEVFKAAARTGTALEVSSFPDRLDL
                     RDEHVLWAKRHGVKFAVDTDAHSTLHLDNLRFGIGTAQRGWLTKDDVINAWPITKLRR
                     FLRKGR"
     gene            11692..12282
                     /locus_tag="VF097_01390"
     CDS             11692..12282
                     /locus_tag="VF097_01390"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX6261480.1"
                     /translation="MTRPLPRDFYERSSIALARDLLGKVVVSELGGTRTAGRIVEVEA
                     YRPDDPASHSFRGPTPGNQTMFGPPGHAYVYVSYGIHRCMNAVARSGSAVLLRALEPL
                     HGLDAMAARRGLDQERLLCAGPGRLCQALGVTRGEDGVDLTAGEGLWITEGPPPRRVG
                     RTPRIGISVGGDRPWRFVEEGSPYASRRATASPAGG"
     gene            complement(12257..14686)
                     /locus_tag="VF097_01395"
     CDS             complement(12257..14686)
                     /locus_tag="VF097_01395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX6261481.1"
                     /translation="MSPPSPRLVRLALAASLLAGACGLPTLEAERARPRPLPQTSFIY
                     ASDGTLLTALHAEQDRVVIGPRLIPRVMKDAVVAVEDQRFWTHGGVDLKAMIRAAAAN
                     VREGAIVQGGSTITQQYVKNTITGDERSIDRKMKEALLAFQLEQEMSKEEILAGYLNT
                     VYFGQGAYGIQQAAREFFSKPARKLNLVESATLAGMIASPATYDPVDNPKRAIRRRND
                     VLERMYEQGTLERDRYLRARTRRLGLRLRPEDSEYPAAHFVEYVKRQILTSPRFGRTY
                     TQRYSFLFGGGLRIQTTIDLEMQAIAEDAIDGILSQPGDPYGALTAVEPDTGFIRAMV
                     GGRDFFAPPKEERFAKVNLATGGSTGRQAGSSFKPFALVAALENGISPQQIYQGGTSI
                     ALTDSVCRNSPTDPWVVQNYEGSAYGSVTLEAATVASINVVYAQVIRDVGPRQVVDAA
                     RRMGIRSTLRPFCSSVLGSNEVNTLEMASAYGTLANLGTHIAPVAIERIEGSDGRVIF
                     EANPRETQAVSPAVAWAATDILKGVIERGTGVAAGIGRPAAGKTGTAQQWRDAWFVGY
                     VPQLSAAVWVGFPQGQISMVPPTTRIRVTGGSFPAQIWRSFMLRATQGLPPRDFQQPP
                     SDLVEVAIDVTQGCVATELTPSENIRIVEFIAGTQPTRKCVVARPEISRVPSVIGMTV
                     STAYELLEGAGFAVVQRTEYDPSYAPGTVIAQEPAAGSQTETGVEITITVSTDNPPLV
                     DVPDVVGLYEGKAVAVLEEAGFAVQVVEADRPEKGTRPRKVLTQTPEPGRERPLGSTV
                     TITINPPPAKR"
     gene            14738..>15694
                     /gene="tyrS"
                     /locus_tag="VF097_01400"
     CDS             14738..>15694
                     /gene="tyrS"
                     /locus_tag="VF097_01400"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX6261482.1"
                     /translation="MDPKEQLRFLLSGAADVIPVEELEERLASGRPLRVKLGIDPSAP
                     DIHLGHAVVLRTLRRFQDLGHTAVLIIGDFTGLVGDPTGRTETRPTLSPQQLEENAQT
                     YLAQVGRVLDVERAEIRRNGEWLAPLTFADVARLAGAVTVAQLLDRDDFQSRYRQGRA
                     ISLTEFLYPVMQAYDSVAIHADVEMGGTDQTFNLMIGRDVQRAHGQDPQVVFTVPLLT
                     GVDGTRKMSKSYGNHIGVDDPPEEQFGKTMRIPDELLVEWFRLTTDVPPDELEAIERG
                     LADGSLHPGETKRRLAREIVTLYHGVDAAEAAEARFDRVHREG"
BASE COUNT         2249 a         5475 c         5732 g         2238 t
ORIGIN      
        1 gcgccgccgc cgttcgccgg cgcgtttcgt atggaggtga ttcggcgtac cggttgaacc
       61 ccgcatcaac gagcggatcc gcgctccgca ggtccgcctg gtaggacccg atggcgggca
      121 gatcggcatc gtctccacac gcgacgccct gcgccaggcc gacgagctcg atctcgatct
      181 ggtcgaggtg gcgccgcagg cgaagccgcc ggtcgcccgg atcatggact acgggaagta
      241 caagtacgag cgcgacgtcc ggcagaagga ggcgcggaag cggcaggcgc gggtcgaggt
      301 caaggagatc aagttccgtc ccaagatcga cacccacgac tacgagacga agaagggcca
      361 cgtcgtgcgc ttcctcacgg cgggcaacaa ggtgaaggtg acgatcatgt tccggggacg
      421 cgagatggcc cacacggagc tggggaggaa gatcctcgac cggctggtgg gggacctcga
      481 ggacatggcc caggtcgagc agatgccgaa gctcgacggc aggaacatgg tgatggtgat
      541 ctcgcccacg aagaaggggt gagggaggac ggcatggcga agacgaagac ccaccgaggc
      601 gccgcgaagc gcttccgtgt gacgcgatcc ggcaaggtcc tgcaccggaa ggcgaaccgc
      661 ggccatctcc tggggaagaa gtcgtcctcc aggaagcggc gtctggccag ggccagcgag
      721 gtgaccgccg agaagcgaac cgtccttcgg ctgctgggga ggcgctgatg ccgcgcgtga
      781 agcgaggcgt ccacgggcgg aagaagcggc gggaggtcct ggcccaggcc aagggctacc
      841 gcggcgcccg ctcccgcagg taccgggtgg cgaaagagca gctcctccac tcgggcgtct
      901 acgcctaccg tgaccgtcgt cggaggaagg gcgagttccg ccggctctgg atcacacgca
      961 tcggcgcggc ctccaggcag cacgggctct cctactcccg gttcatgacc ggcctgaagg
     1021 ccgctgaggt cgacctcgac cggaaggtcc tggccgacat cgccgccacc gaccccgagg
     1081 gcttcgccgg actcgtcgag gtcgcccgcc gagggctggg ggagtaggcg gggcgggcgg
     1141 ccgcggggcc gtgatcacct ccaccaagaa cccgcgcatc gtcgcggcgg ccaagctgaa
     1201 gaagcgcgcc ttccgggatc gggaggcgcg cttcctcgtg gagggggccc aggtcaccct
     1261 ggaggcgatc gatgccggcc gcgtggaggt cctcttccac acgatggacg agcacggccg
     1321 ggtcgacgct ctcgtggacc gagcccgggg gtcgggggtg cggaccgagg cggttaccca
     1381 gcaggtcgtc gagcacctca cgtccaccgt gaccccgcag ggcatcgtcg gggtggccac
     1441 ctacgtggac gtccccctcg gggagctgca gcccgatccc tcgctcgtcg cggtgctgag
     1501 ctcggtccga gaccctggca acgcggggac gatcctccga acggccgacg cggcgggggc
     1561 ggacggggtg gtgttcggcc gcgagtcggt ggacgtctac aacgccaaga ccgtgcgagc
     1621 gtcggcgggg tcgctcttcc acctgccggt cttccgggac gtggacgtgg tcgaggccct
     1681 ggaggggctt cgtcgcggag ggctgcaggt tctggcgaca gccgcggacg gggagcggac
     1741 catctacgag gccgacctct ccgcgccgac tgcgctcgtg ttcgggaacg aggcgcacgg
     1801 cctggagccg gagattcggg cgctggccga cgtcaccatc cgcgtcccga tccgcggccg
     1861 agccgagtcg ctcaacctgg ccgcctccgc ggccgtgacg ctgttcgagg ccgtccgagc
     1921 ccgagaggcg cccgacgggc tcgcccagat cgccgggctg atcggccccg ccggccacga
     1981 cgtccgctcc ccgctcaccg cgctcacggg cttcgtgacc actctggcca gacggtggga
     2041 cgggttctcc gacgaggagc ggcgggagat cgtcgaaggg atggtcctgg acggccaccg
     2101 gacggccgcg ctcgtccgcc tcgtggtcga cgctgtacgc atcctctcgg gggcgcccct
     2161 cacctcgacg agcgaacgag cggacgtcgg cgcggccgtc cggtggctgg ccgagctctt
     2221 cgctgccagc gaggacttcc ccgacctcga ggtggagggc gaggcgcggg cgcgctctga
     2281 tcccgaccgg atccgcgccc tgctgttcgg cctcgtggac ggcgccatgt ggtgggggag
     2341 cgagggtccc gtgtccctcc gcctctccga agagggatcg tacgcggtgg tggaggcatg
     2401 gcgaacgggg gaccgtcacg gcgacgagga gccgacggac gtcctcgggt cccccgagca
     2461 cggcggccgc gtcagcctgt acgcggccag gctcgtggcc gagcgcctgg gggggtcgct
     2521 ggaggtcgag accgacggag ggatccggct ccggctgcgc cttcccgtct aggtctcccg
     2581 ctcgatcagc tcgcggagcg tctccaggtc ccgacggaac gttcgatgga tcaccggcga
     2641 catcagacgc atcccgatcc gtccgagggg gccgagcggc ggacggagcg cctcgcgcca
     2701 gaagaggtgg gagccgacgg ccgtcacccc cagctccatc aggccccgcc cgctcaccca
     2761 ccccaggtgc tcgatctcga gcacggccgg cggctcccac ctcgttacgc ggaccaggtc
     2821 cctcgtcgtg atccccagga tcgagatggt ggcctccgct tcgacgccca cgccctcccg
     2881 cggctcgccc aggacccgga aggcccgggc ctcctccatc cagcggtcga gcccctccca
     2941 gtccacgagg tagggccaca ccacctccgg cggccggggg atctccacct ccatcgtgaa
     3001 gatgagcagg ccgctggggt cggcgtccga ccagcggatc cctagcaccg gcgccgcgtc
     3061 gggatggtca cggtcagtag actagccctc gacatggacc ccggggagac gatcgccgcc
     3121 ctcgagcggg agcgcgagcg gggcctcgcc gcgatccgcg aggccgcctc gctcgaggac
     3181 ctgcgggaag cggaggtcgc cgttctgggg agacgggctc ccgtccccac gatccagcgg
     3241 tcgctcggcg aggctgcgcc ggacgaccgg cccgggctgg gaaaggcgat caacgaggtg
     3301 ttcgaggcct tccgcgaggc cctctcggac cgcgagcggg agctggcggc cgagcaggag
     3361 cgggcggtgg gcgaggtgga cgccgtcgac gtcacacttc ccggcaggcg ccttccgatg
     3421 ggggcgctcc acgtcctgac ccagaccgag cgggcgatgg tcgatgcgtt cgtggccctc
     3481 ggctaccgcg tgatggaggg gccggaggtg gagaccgact ggtacaactt cgaggcgctc
     3541 aacatcccgc ccgatcaccc ggcgcgtacg ccccaggaca ccttgtacct cgacgtgccg
     3601 ggcgcctccg acctgctcct ccgcacccag acctcgccga tgcagatcaa ggctatgcag
     3661 gcccagccgc cgcccctata cgtggtcgtg cccggccgca cgtaccggaa cgacacgctc
     3721 gacgccacgc actccccggt gttccgccag gtggaggggc tggccgtgga cgagggactc
     3781 tcgctcgccg acatgaaggg cacgctcgag gcctgggccc gagcgatgtt cagcgaggac
     3841 cggcgcgtcc gtctgcgccc ggactacttc ccgttcgtgg agccgggggc tcaggtagac
     3901 gtcacgtgct tcgcctgcga cggcgagggc tgccggatct gcggcaccgg ctggctcgag
     3961 atcatggggg cgggcatggt gcaccccaag gtcctggaga acggcggcgt ggaccccgag
     4021 cgctacacgg gctacgcctg gggcatcggc gtggagcgcg tggcgatgct gcgtcacggc
     4081 atccccgaca tccgcctgtt ctacgagggg gacgtccggt tcctggagca gttcgctcgg
     4141 ggctgggcat gagggtcccc tactcctggc tgcgggagat cgtcccgacg cgcctgtccc
     4201 ccgaggaggt cgccgtcgcg ctcaccgagc acggcgtcga ggtcgaatcc gtccttcggc
     4261 cctgggaggc gctgaccggc gtcgtcgtgg cccgggtgct cgaggtgacc gatcacccga
     4321 gggcggaccg cctgtccctg gctacggtgg acgcgggcgg ggaggagcgc cgcctcgtgg
     4381 tcggcgtgcg gaacatggga ccgggggacc tggtgcccta cgcgcccccc ggcgcgaccc
     4441 tgccgggcct cgaccgcccg ctcgagcggc ggacgatcag gggggagacg tccgatggga
     4501 tgctctgctc cccgaaggag ctcgggatct ccgccgacca cagcggcatc ctgatcctcc
     4561 ccgaggggga gtcggggcag gacgtcgcgg ggctgttcga tctcgacgac gcggtgatcg
     4621 acatcgaggt cttccccaac cgtcccgatc tcctctcggt cctcggcgtg gcccgcgagg
     4681 tggccgccat caccggcgac gagctgcggg tgcccaccgc gtcggtgctg gaagcggacg
     4741 agaagggggc ggcggtcgcc tccgtgcagg tggacgaccc gaagcggtgt ccccggtacc
     4801 tggctcgggt gatccggggc gtcgcgatcg ccccgtcacc cctgcgggcc cagatccgcc
     4861 tcacggcgtg cgggatgcgc cccgtctcga acgtggtgga cgccacgaac tacgcgctcc
     4921 tggagctggg gcacccgatg catcccttcg acctccggac cctcagcggc ggcgcgatcc
     4981 tcgtgcggcg ggccaccgaa ggggagcggc tcgtgacgct ggacggcgtg gagcgggcgt
     5041 tcacctccga ggacctgatg atcgccgatc ccgagcgggc cgtcgcggtg gccggggtga
     5101 tgggcggcga cgccaccgag gtgggggacg ccacgaccga cgtcctgttg gagagcgcct
     5161 acttcgaccc gctcggcgtg ctccggacgg cccggcgtct gggggtgcgg accgaggcgt
     5221 cgatccgctt cgagcgaggg gccgacccgg aagcggtggc cgacgccgcg ctccacgcgg
     5281 ccgggctcat cgcggcatgg gccggcggcg ccgtcctctc gggcgagatc gacgtgggac
     5341 gcccgccccc gcgccgccgg gtcgcggtgc ggccgtccag ggcgagcatg ctcctgggca
     5401 tggaggtctc cgccgcagac gtcctggacg tgatgggccg gctggggatc gatggagagg
     5461 agcgggaggg cagcgtcgag atcgaggtcc ccggccgccg ggtcgacctc gagatcgagg
     5521 ccgatctgat cgaagaggtg gggagggtgc gcggttacga cgccctgccc tccacccttc
     5581 caggggtggc tcaggcgggt gggctcgatc ccgagcagcg tgccctgcgg cgcaccgagg
     5641 acgcgctggt ccgcgcgggc ctcatgcaga tccactcggt gagcttcgcc tccggcgagg
     5701 acctcgagct cttcgaggac gagcgggccg cgggcgtccg ggtggccaac cccgtctccg
     5761 aggagcagcg cttcctgcgc tcgagccttc ttcccggcct cctgcgggcg gccgccctct
     5821 cgatcggcca ccgccgggcg gcggtgcgcc tcttcgaacg agggcacgtg atgcggctgg
     5881 aggaggaggt ggtcgaggag gacgcggtgg ccgccctgct gaccggcccg gccctcgagg
     5941 tgtggccggg ggagcgccgc ccccaggacg tcctcgatgc gaagggcgcg atggagcatc
     6001 tcctcgagga gctcggggtg gacggatgga gcctgggcga tccgccgggg ccgccgtacc
     6061 atccgggccg gtcggcccgc gtgctgctga acggggagcc gatcggcgag ctcggcgagc
     6121 tgcacccctc agtgggggag gggttcgacg tccccgggcg tgtggccgtc ttcgagctcc
     6181 gcgccgcccc tctcgtggct gcgggcgcgg gtctcgtcga gctccgaccc gtctcccgct
     6241 tccccccgat ccgccgcgac ctcgcgttcg tcgtggaccg gtcggtggcg gccgcggacg
     6301 tgcgggcctc gctcgtggac gcggcggggc cgctgctcga ccgcgtcctg ctcttcgacg
     6361 tgtacgatgg cgagccgcta cccgagggga agcggtccct ggcgttcgcc gtggacctcc
     6421 gggccgccga ccgcacactg accgacgacg aggccgagga ggtcgtggcc gcgatcgagg
     6481 cccggctggc gtcgggcttc ggggccgagc tgagagcggg gtgagatgaa ggagaacctc
     6541 acgagggacg aggccgccgg ccgcgcggcg ctcgtccggg acgtcgccta cgagatccac
     6601 ctcgacctca cgggcgccga gcggttccgg tcgcagaccg tgattcgctt cgtcgccgag
     6661 ccggggacct ccacgttcct cgactgcacg gcggtggagg tgcacgccgc ggagctgaac
     6721 ggagagtccc tccccgaggg cgccgtcacc gagcaccgga tcgcgctggg ccctctcgcc
     6781 gggaggaacg aggtgcgcgt ggtggccgac atggcctatc gggcggggga gcacggcatg
     6841 accaggttcg aggatcccgt ggacggggcc gtgtatctgt tctccgacgc ggagccgttc
     6901 ggcgctcacc gcatctaccc ctgcttcgac cagccggacg tgaaaggcac gttccgcatc
     6961 gacgtgaccg cgccggcctc ctgggaggtg gcctcgaaca tggccgcgga ggccgagccc
     7021 gaggccgagg gcggcgcccg gcgctggtcg ttctccgaga gccccaggat gtcgacctac
     7081 ctcgcggccg tcgtcgccgg ccccttccac gtggcgcgcg atcgccacgg cgagatcgat
     7141 ctggggatct actgccgggc gaccctcgcc cagcacctgg acccggacga gatcctcgag
     7201 gtcacgaagc agggattcga cttcttcgag aaggccttcg ccacgcccta cgccttcggg
     7261 aagtacgacc aggtcttcgt ccccgagtac gcggccgggg cgatggagaa cccagggctc
     7321 gtcacgttca acgagatcca catcttccgg tccagggtga ccgacgcgag gcgggagggg
     7381 cgtgccgaga cgatcctgca cgagatggcg cacatgtggt tcggggatct cgtgacgatg
     7441 cgctggtggg acgacctctg gctcaacgag acgttcgcca cgttcgcctc gatgctggcc
     7501 atggagcgag ccacgcgctt cacggagccg tggacgagct tcgcccacac cgagaagccg
     7561 tgggcgtccc gacaagatca gcttcccact acgcacccta tcgtggccga cgtccccgac
     7621 gtggagtcgg tgttcctcaa cttcgacggc atcacgtatg cgaagggggc cgccgtgatg
     7681 cgccagctgg tggcctgggt gggcctggag cggttcctcg aggggatgcg ggtctacttc
     7741 gagcgccacg cgtgggggaa cgccgcgctc gcggacttcc tggccgtcca cgaggagacc
     7801 tcgggacggg atctgggggc ctgggccgag gtctggctcc gggcctccgg gatcaacgcg
     7861 ctcacccccg aggtcggcga gggcgtggcg atcctccagg agggggagcc cctccggccc
     7921 caccgtctcg cggtcggggc cttctcgccg gagggtgaga agctggtccg ggccggccgc
     7981 tcggagcttg acgtcacggg ggcgcggacc gaggtgccgg agctctcctc ggacgcggcg
     8041 ctcgtggtgc tgaacgacga ggacctgacc tatgccaagg tccggttcga cggacgctcc
     8101 ctcgagaccc tccgggaggg cctctcccgg atcgaggatg ggctcagccg ggcggtctgc
     8161 tggcgcgcgc tctgggacat gacccgcgac ggcgaatggc ccgcccgcga gttcctggcg
     8221 accgtgctgg accacgtcca cgccgagacg atgccgtcgg ccgtccagcg catcctgggc
     8281 caggccggag cggccgtttc gctgctcggc gatccggcga accgcgacgc ggcgggaagc
     8341 gccatggcca cggcggccct ggaggcgctg cgggccgcgg cggcgggcag cgacctccag
     8401 ctgatctggg cccggacgtt cgccgccgcg gcccgcaccc ccgagcacct ggatctcgtg
     8461 ctcgccctgc tcgacgggga ggagcggttc ggcgggctcg ccatggacac ggatctgcgg
     8521 tggcacctgg tgcgctcgct ggcctccgcg ggacgggcgg acgagggcct gatcgccgtg
     8581 gagcaggagc gggaccccac ggacgaggga gcgcgcgagg cctccggggc ccgggcggcc
     8641 cgccccaccg cgggggccaa ggccgaggcg tgggaggcgc tgaccggtcc cgagccgccg
     8701 cggctagcgt cggctcgggc catcctggcc ggcttccagg atcccgagca ggaagaggtc
     8761 ctcgccccct acgcccgccg ctacttcgac accctggacg acgcgtggga gcggttcgac
     8821 ctctccctgg ccctctcgtt cgcccgcggc gccttcccat cggcgatcgt ctccgaggag
     8881 accgtggcca tgaccgacga ggtcctggcc cgcgaggacc tccccggccc gatccgtcgg
     8941 gtcctcctcg agggacgaga cgacctcctg cgggcgctgc gggctcgcgc caccgacgcg
     9001 gcggccggct gaggttcgac cctcggcggg ggcccgcgct agactccggc ccgtggcgcg
     9061 ccacttcctc tcgatggacg acctcacggc cgaggagctg caggggctgc tcgacctctc
     9121 ccgcaaggtg aaggcctcgc ccgaggacca cgccgcggcc ctcggcggcc gggcggtggg
     9181 catgatcttc gagaagccct cgacccggac cagggtgtcg ttcgaggtcg gaatcgccat
     9241 gatgggcggg cacccgctgg ccctctcctc gagcgagctg cagctcggcc ggggcgagac
     9301 gatcgaggac accggccggg tgctctcccg ctacctcgac gcggtggtcc tccgcacgtt
     9361 cgggcaggag cgtctcgagg ccctcgcgtc cacctcgacg gtcccggtgg tgaacgcgct
     9421 ctcagatctc gagcacccgt gtcagtgcct cgcggacctc ctcaccgtcc tcgagcagaa
     9481 gggagagctc gccgggcaga ccctcgcgta cctgggcgac ggcaacaacg tcgcgcactc
     9541 gctgctgctg ggcggggcca aggtagggat gcacgtgcgg gtggccacgc ccgccggcta
     9601 tgagcccatc ccccagatcg tggagcgggc cgccgagatc gcgacggtga cgggagggag
     9661 cgtccaggtg acggccgacc cgatcgcggc ggcgaccaag gcggacgtcc tctacacgga
     9721 cgtctgggcc tcgatggggc aggaggccga ggccaccgag cgggtgctgg tgttcgcacc
     9781 gtaccggctc gacggcgaga aggtggccgt ggccgcagag gacgtggtcg tcctgcactg
     9841 cctgccggcg caccggggcg aggagatcac cgacgaggtg atcgacggcc ctcactcggt
     9901 cgtctgggac caggccgaga accggctcca cgcccagaag gcgctgctgc tccggctgct
     9961 cggcatcgcc tgagccgtgg ccaggtcgaa cgacgaggtc gccgcgctcc tgcaggagta
    10021 ctcggacctg ctggccatcc tgggggacga cagcttcaag tcccgggcct acgagaaggc
    10081 cgcgcgttcg gtggagggct accacgccga ggtctcgacc ctcgacccgg ccggctgccg
    10141 cgccatcccc aacgtgggcg cctcggtggc cgagaagatc caggaggtcc tccggacggg
    10201 gaccttccag gcgctggagg atctgcgagc ggacatcccc gacggcgtgc gggccttgct
    10261 gggcatcccc acgctcggcc ccaagaaggc cctgaagctc tacgaggagc tccagatcgc
    10321 ctcccccgag gagcttctcg acgccctcca cgagcagcgg atccgcgagc tgccgggctt
    10381 cggccccaag accgaggaga acctcctgcg gggcatccag atgctgcagc gctccggcgg
    10441 ccgggtccag atcggcgttg ccatggacct ggccgaggag ctgctcggcc acctgaccga
    10501 ggtcccccag gtccggcggg ccaccttcgc aggatcgctc cggcggatgg cggagacggt
    10561 cggcgacatc gacctcctag cggcctccga ggacccgatc ccggtgatgg gcgcgttcgc
    10621 ctccggcccg tacgtccgcg aggtgctggc caaaggggag acgaagacct cggtgctcac
    10681 gacgaagggc ctccaggtcg atctccgggt ggtggagccc gccgtctggg gggccgcgct
    10741 ccagtacttc accggctcca aggcgcacaa cgtccacgtc cgagagatgg cggtccggaa
    10801 gggcctgaag ctgtccgagt acgggctgtt ccgagccaag agcgggaagc tgatcgtggc
    10861 cgagaccgag gaggaggtct acgagcggct cggtctgcct tggatccctc cgcccctccg
    10921 cgaggaccgg ggtgagatcg aggcggcgct cggggacggc cttcccacgc tgatcgagcg
    10981 aagggacgtg aagggcgatc tccacacgca caccgatctg accgacgggc tggccacgct
    11041 cgaggagatg gtggccgcag ccgagcgggt cggctaccgg tactacgcga tcaccgatca
    11101 cgccgagctc ctctatatgc agcggatgac gcaggagaag gcgctggccc agcgggagcg
    11161 gatccgggag ctcgacgagc ggtccaaggt cacgctgctg cacggctccg agctgaacat
    11221 ccagccggac gggtccgtgg actgggacga ggagttcctc tcggggttcg acgtgctcgt
    11281 ggcctcgatc cactcgcacc tcacgatgcc gaaagcggag atgaccaagc gcatgatccg
    11341 ggcgatggag cacccgtgcg tgaacgtcat cggccaccct accgcgcgcc agatcgggcg
    11401 gcgacccccc atcgaccttg atctcgagga ggtgttcaag gccgcggccc gcacgggcac
    11461 cgccctcgag gtgagctcct tccccgaccg cctcgatctg cgcgacgagc acgtcctctg
    11521 ggcgaagcgt cacggcgtca agttcgcggt cgataccgac gcgcactcca cgctccacct
    11581 ggacaacctc cgcttcggca tcgggaccgc gcagcgcgga tggctcacga aggacgacgt
    11641 gatcaacgcg tggccgatca cgaagctgcg ccgattcctg cggaagggcc ggtgacccgg
    11701 ccgctcccgc gagacttcta cgagcgctcc tcgatcgcgc tcgcccggga cctcctgggg
    11761 aaggtcgtcg tgagcgagct cggggggacg cgcaccgcgg gaaggatcgt ggaggtcgag
    11821 gcgtaccgtc ccgacgaccc cgccagccac tccttccgcg gccccacccc tggcaaccag
    11881 acgatgttcg gtccgccggg ccacgcgtac gtgtacgtct cctacggcat ccaccgctgc
    11941 atgaacgcgg tcgcccgatc gggcagcgcc gtgctgctgc gggccctgga gccgctccac
    12001 gggctcgacg ccatggcggc ccgccgcggc ctggaccagg agcggctgct gtgcgcaggc
    12061 cccgggcgcc tgtgccaggc cctcggcgtc acgcgaggcg aggacggcgt ggacctcacg
    12121 gccggcgaag ggctgtggat caccgagggt ccgcccccgc ggcgcgtggg gcggacgccc
    12181 cgcatcggga tcagcgttgg cggcgacagg ccctggcggt tcgtggagga ggggagcccc
    12241 tacgcctcgc ggcgggctac cgcttcgccg gcggggggtt gatcgtgatc gtcaccgttg
    12301 agccgagcgg gcgctccctg ccaggctcgg gggtctgggt cagcaccttc ctcggccttg
    12361 tccccttctc cggtcggtcg gcctcgacca cctgcaccgc gaacccggcc tcctccagca
    12421 cggccacggc cttcccctca tagagcccga ccacgtctgg gacgtccacg agaggagggt
    12481 tgtctgtgga gacggtgatc gtgatctcga cgccggtctc ggtctggctc ccggccgccg
    12541 gctcctgcgc gatcacggtc ccgggagcgt agctcgggtc gtactcggtg cgctggacca
    12601 ctgcgaagcc ggccccctcg agcagctcgt aagccgtgct gaccgtcatc ccgatgaccg
    12661 acggaaccct cgagatctcg ggacgggcca cgacgcactt ccgcgtgggc tgggtcccag
    12721 cgatgaactc cacgatgcgg atgttctccg agggcgtcag ctccgtggcg acgcacccct
    12781 gggtcacgtc gatcgccacc tccaccaggt ccgagggcgg ctgctggaag tcccggggag
    12841 gcaggccctg cgtagcccgc agcatgaacg atcgccagat ctgcgcgggg aaagatcccc
    12901 ccgtgacgcg gatcctggtg gtggggggga ccatcgagat ctggccctgc gggaacccca
    12961 cccagacggc ggccgagagc tggggcacgt atccgacgaa ccacgcgtcg cgccactgct
    13021 gagcggtccc agtcttcccc gccgccggcc gcccgatgcc cgcggccacc cccgtcccgc
    13081 gctcgatcac gcccttcagg atgtcggtgg ccgcccaggc cacggcgggg ctcacggcct
    13141 gcgtctccct ggggttcgcc tcgaagatca cgcgcccgtc cgagccctcg atcctctcga
    13201 tggccacggg ggcgatgtgg gtccccaggt tcgcgagcgt gccgtaggcc gaggccatct
    13261 ccagggtgtt cacctcgttc gaacccagca ccgatgagca gaaggggcgg agggtgctgc
    13321 ggatccccat cctccgggcg gcgtccacca cctggcgcgg tccgacgtcg cggatcacct
    13381 gggcgtagac gacgttgatg ctggccaccg tggccgcctc gagcgtcacg ctcccgtagg
    13441 ccgaaccctc gtagttctgg accacccacg gatcggtggg ggagttccgg cagaccgagt
    13501 cggtcagggc gatcgaggtt cccccttggt agatctgctg gggcgagatg ccattctcca
    13561 gggccgccac gagggcgaac ggcttgaacg aggaacccgc ctgccgcccg gtcgagcccc
    13621 ccgtggccag gttcaccttg gcgaaccgct cctccttggg aggagcgaag aagtccctgc
    13681 cgccgaccat cgcgcggatg aacccggtgt cgggctcgac cgcggtcagc gccccgtaag
    13741 ggtcgccggg ctgggagagg atgccgtcga tcgcgtcctc ggcgatggcc tgcatctcga
    13801 ggtcgatcgt cgtttggatt cgaaggcctc ctccgaagag gaagctgtac cgctgcgtgt
    13861 acgtgcggcc gaagcggggg ctcgtgagga tctgtcgctt cacgtactcg acgaagtggg
    13921 cggccgggta ctcggagtcc tcgggtcgga gccggaggcc gaggcgcctg gtcctggccc
    13981 gaaggtagcg gtcgcgctcc agcgtcccct gctcgtacat gcgctccagc acgtcgttcc
    14041 ggcggcggat ggctcgcttg ggattgtcca ccgggtcgta ggtggccggg ctggcgatca
    14101 tcccggccag ggtcgccgac tccacgaggt tgagcttcct ggccggcttg gagaagaact
    14161 cgcgggccgc ctgctggatc ccataggcgc cctgaccgaa gtagacggtg ttgaggtagc
    14221 cggcgaggat ctcctccttg ctcatctcct gctcgagctg gaacgcgagg agcgcctcct
    14281 tcatcttccg gtcgatcgac cgctcgtccc cggtgatggt gttcttcacg tactgctggg
    14341 tgatggttga gccaccctgg acgatcgccc cctcgcgcac gttggcggcg gccgcgcgga
    14401 tcatcgcctt gaggtccacc cctccgtggg tccagaagcg ctggtcctcc accgcgacca
    14461 ccgcgtcctt catcacccgg gggatgagcc tcggcccgat cacgacccgg tcctgctccg
    14521 cgtggagggc cgtgaggagg gtcccatccg acgcgtagat gaacgaggtc tgaggaaggg
    14581 gcctggggcg ggcccgctcg gcctccagcg tcgggaggcc gcaggcgccg gcgagcagcg
    14641 aggcggcgag ggcgaggcga acgagcctcg gggaaggggg gctcatagcc ggattgtacg
    14701 cccgggcccc aggttcccct ggtagccttg cgcgcggatg gatcccaagg agcagcttcg
    14761 cttcctcttg tccggcgccg ccgacgtgat ccccgtcgag gagctggagg agcggctcgc
    14821 gagcgggagg ccgctccggg tcaagctcgg catcgacccg tcggcccctg acatccacct
    14881 cggccatgcc gtcgtgctcc ggacgctccg caggttccag gacctgggcc acaccgccgt
    14941 cctcatcatc ggggacttca ccgggctcgt gggcgatccg accgggcgga ccgagacccg
    15001 tcccacgctc tcgccccagc agctcgagga gaacgctcaa acctacctgg cccaggtggg
    15061 acgggtgctg gacgtggagc gtgccgagat ccgccgcaac ggtgagtggc tggcgccgct
    15121 gaccttcgcc gacgtggcgc gcctggccgg ggcggtcacg gttgctcagc tgctggaccg
    15181 ggacgacttc cagtcgcgct atcgccaggg gagggcgatc tccctcacgg agttcctgta
    15241 ccccgtgatg caggcgtacg actcggtggc gatccatgcc gacgtcgaga tgggggggac
    15301 cgatcagacg ttcaacctca tgatcggccg ggacgtgcag cgcgcccacg gccaggatcc
    15361 ccaggtcgtg ttcacggtcc cgctgctgac gggtgtcgac gggacccgca agatgtcgaa
    15421 gtcctacggc aaccacatcg gggtcgatga cccgcccgag gagcagttcg ggaagacgat
    15481 gcgtatcccc gacgagctgc tcgtggagtg gttccggctc acgaccgatg tgccccccga
    15541 tgagctggag gcgatcgagc ggggactcgc cgacgggtcg ctccacccgg gggagacgaa
    15601 gcggcgcctg gcccgcgaga tcgtgaccct ctaccacggg gtggacgcgg ccgaagcggc
    15661 cgaggcccgc ttcgaccgcg tccaccgcga gggc
//
LOCUS       DASUTZ010000031        12729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1303913, whole genome shotgun sequence.
ACCESSION   DASUTZ010000031 DASUTZ010000000
VERSION     DASUTZ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12729
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303913"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..995)
                     /locus_tag="VF097_01405"
     CDS             complement(132..995)
                     /locus_tag="VF097_01405"
                     /inference="COORDINATES: protein motif:HMM:NF014646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HEX6261483.1"
                     /translation="MTEPERLSISEVSRFVDVPIPTIRSWERRYGVPAPARTGGGHRR
                     YTREEVDLIRAMRDEIVRGASARDAARRIRLAPAPAIEPTPHLETLLDRVLDLDDAGI
                     RGSLDQAADGLGIERAVVEVALPALREVGDRWAGGRCDVAQEHLATNEVRSWIARLGR
                     TAPPPFRPAPILLAAAPDETHATGLEAFALLLARRGWPTVVLGARIPVDSLLRTVSKV
                     GPAAVVLSSQWTRARRSALEALEAVAATSVPVFYAGAAFAVPSKREGVPGVYLGDALL
                     DSLDELERKVA"
     gene            complement(1103..3403)
                     /locus_tag="VF097_01410"
     CDS             complement(1103..3403)
                     /locus_tag="VF097_01410"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX6261484.1"
                     /translation="MKEIRRSVFHRLGGFAVRRRWWVVAVWVAALVAAFVPLRELGDR
                     LSQGGFEVPGSESERVQLAVQSEFTGQFEFSDQLVMRSESVTVDDPRFREAFGDVRAA
                     LLEAPGVGDVTDPYATPERSIGPDGRTLVSTVGLTDDQDEALEHADELHDAVHGAVPH
                     GGDIEAYITGAAPFYSEFSEVTQHDLERAEAIALPISLLILIAAFGSLVAAGMPLALA
                     LFGLAISFGIISLVAAATTVSIFTQNVASMIGIGVGIDYSLFVVSRYRQELGEGHDVR
                     EAVARAMATSGKAVMVSALTVVIALSGTLLVDIAAFRSMGLGAMIAVSIAGLAALTIL
                     PALLGFVGRKINSLRLGRRRLKDEERSGGLWHRWAMLVMRRPWFALIGSLALLALLAW
                     PARNLRLGTAGPDIFPPDSEPRVAAEAVAEAFGEGRAAPMQILIEHPEGVLEDGFEDV
                     AEVAGALQSDPSILQVDSIATLAPVASVEEARAVAASPEAAPVIQTMVGNDGKATLLR
                     VVTADEPGSDEVRELVRRLREDLPALLPAGAEVAVGGDPGLTVDLNEEIESAIIPVVA
                     MVLTLSFFLLLLFFRSILLPLKAILMNLASVLATYGIVVFIFQEGHFQELLGFSATGH
                     IESFVPLFLFTILFGLSMDYEVFLLARVREEYLRTGDNTEAVGWGLEHTARIITSAAA
                     IMVTVFGAFAFASLVPIKSMGFGLAAAVFLDATLVRVVLVPATMRLMGDWNWWLPGWL
                     DRILPNVSLEGVGPEPPASAEAPAPA"
     gene            complement(3400..4008)
                     /locus_tag="VF097_01415"
     CDS             complement(3400..4008)
                     /locus_tag="VF097_01415"
                     /inference="COORDINATES: protein motif:HMM:NF041196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="HEX6261485.1"
                     /translation="MARRTDRGEETRSRILAAAAAAFAERGYAGTSMNELVRATGLTK
                     GAFYFHFDSKEDLALEVFRWKHEEWQAMVAEAIEEGARALDQLVAVMGAVTECVEADP
                     SARSLGRLAGELSGDPRLASVLDVQFRSWVEFSTDLLRRAQEEGDVRPDLDPREVSEV
                     VVAGFIGLEHVSGTRGAGDLRRWSEALTGLVLDAIRAKEQVT"
     gene            4103..4408
                     /locus_tag="VF097_01420"
     CDS             4103..4408
                     /locus_tag="VF097_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HEX6261486.1"
                     /translation="MLQVTEAAVGVIRQIRMDTPGVSDGAKARIQLVASGEEEQPGLG
                     FGFSDEPIEGDHKVAEKEDIQVFLAPELVEPLSAAVVDAVPAAEGQQLVLREQAEEG"
     gene            4434..5267
                     /locus_tag="VF097_01425"
     CDS             4434..5267
                     /locus_tag="VF097_01425"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX6261487.1"
                     /translation="MPEYPEVQALAERLDDLFHGAALEGIDAIQFSALKTVEPSPESL
                     TGRALEGVGRRGKWLLLDLGGPRIALHLSQGGRVMLEDPPKRSRPRGGVVRLRFAGRP
                     ALMVREFGTERRAAWHVLAPGDEGPLAVLGPEAASERFATWLASADDGRRVHTILRDQ
                     RTVAGIGRGYADDILHRAKLSPFASLAKLDPEERGRLSEAVRDVLSEALQEERKRVGG
                     LPPKLGDRFAVHGRWGRPCPRCGGDLRRISYESHEVTYCPRCQTGGKVLADRRFSRLV
                     R"
     gene            5334..6245
                     /locus_tag="VF097_01430"
     CDS             5334..6245
                     /locus_tag="VF097_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6261488.1"
                     /translation="MTHAISTNGLVKTYGSTVALDGLDLEVPAGEVHGFLGPNGAGKT
                     TTIRILLGLLRADRGTVRVLDGDPWEDAVALHRRIAYVPGDVELWPTLTGGETIDLLG
                     RLRGGLNPTRRDELIERFDLDPTKKGRTYSKGNRQKVAIVAALASDSELLLLDEPTAG
                     LDPLMEVVFQDVIRHIKAEGRTVFLSSHILAQVEKLADRISIIRLGRIVQSGTLTEMR
                     HLTRTTIEAHTATPVSGLETIPGVHHLEANDTRVHFAVDGEHLDDAVKHLSSFGIRAL
                     TSHPPTLEELMLRHYGDELESVGAGGE"
     gene            6242..7900
                     /locus_tag="VF097_01435"
     CDS             6242..7900
                     /locus_tag="VF097_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004985424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6261489.1"
                     /translation="MTSAAAAERIPPAGEGTAFTGTGRLAQFILRRDRIRLGVWVGAI
                     ALLVVLFVSSLPQYYTEASQRQARAELMANPGLRAIAGPGYGLDDYTFGAMVAQEFLS
                     WTAIFVALMSFLLMVRHTRAEEETGKAELVRSAALGRHAHTAAPLVVLIGANLVLGGL
                     IAVGLGSLGMEGVSWPSSWLFGAALASIGLVFTAVAAVTSQVSPGARGAAGLAGFGFA
                     VAYLVRAAGDMSEVGGGFLSWLSPIGWAQQTRVYVDDRWWPLLLSVALTAVLVLVAFR
                     LSTRRDFGAGLVQPRPGPARGSGLLSTPLGLAWRLQRTSLMWWGVSMFFFGVGYGALA
                     GEVERYVGELSGAVQEWLEGIGGDAIIDSWLAVLALLVGVTVAVFAVLAVLRPRNEEA
                     AGRAEPVLATSVSRVGWVMSHLAVALAGSVVILALAGLGLGISASAALGDTSLMPRVV
                     GATLVYIPAVWVTVGIGVVLFGLVPRATRLVWFVIAYAGLIGMFAELLGLPDWTVDLS
                     PFGHVPLVPAVPVRWAPLAILTAIAAGLITLGLYGFRRRDLAAT"
     gene            8307..8615
                     /locus_tag="VF097_01440"
     CDS             8307..8615
                     /locus_tag="VF097_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX6261490.1"
                     /translation="MQLTQTAAEKVKELLAQEGHPEAALRVAARPGGCCGYRYSMALT
                     DQIDEADQTVEHHGIRVVVDSESVPLLDEATIDFVDSLERSGFAIENPKAGGGCTCGH
                     "
     gene            8937..10967
                     /locus_tag="VF097_01445"
     CDS             8937..10967
                     /locus_tag="VF097_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX6261491.1"
                     /translation="MTGRRITFYVGLVCIAGLPLVAWLMATNGLSAARIALWTLWPFT
                     AFVVVGQFLPVRIPREGEEEEEITTSSTFSFAILLATGPAPAALALAVGSVASDLARR
                     KPAWKAGFNVAQCTLSILAAHLVLETIGFTYLFQGGSAKVEMHTLNLLAVLLAALAYF
                     LVNNTVTGIALALAQGLPVGPYLRRDFGVQASIAAVLLALSPVVVVAAQHSLLMVPLL
                     GLPMAAVYKGARVWLEKESQEHQAKHDPLTGLPNRSLFYQRVRREIGRRAKKPGSFGV
                     MIIDLDRFKEINDTLGHYAGDQLLRQIGPRMRDVLRDEDVIARLGGDEFALLVPDLPG
                     EMAALQVAERLQEALETPFTVEGLLLDVDASIGIAIYPGHGMSLDDLVRRADVAMYLA
                     KETHSGFELYAPERDQHSLGRLTLLGELRRAIERDQLILYFQPKADMRTGRVVAAEAL
                     VRWDHPSHGLLIPEKFVPLAERTGMMRALTLFVLNSALRQCREWHAVGQPLGVAVNLS
                     ARNLLDLRFPDEVQKLLASWQVAPGWLELEITESAVMADPARAMHVLARLSEMGVGVS
                     LDDFGTGYSSLVYLKRLPVNEVKIDKTFITHMSDAEDDAVIVESTIELARSLGLRVVA
                     EGVETEDVWRRLSGLGCDMAQGFYLSRPMPPPDMGNWLQGAQPSGWGLSVAT"
     gene            complement(10951..>12729)
                     /locus_tag="VF097_01450"
     CDS             complement(10951..>12729)
                     /locus_tag="VF097_01450"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEX6261492.1"
                     /translation="TEPPPAAHDAGPAWSPDGSRVAFSRALPGRTPDLFVVDADGAGL
                     RRLTSGDPLEASPAWSPDGSRVAFERCCPNGSSEIYTVDVEGGVESNLTASPAHEASP
                     AWSPDGARIAFVRAPASGGRDLYTMDAAGDDVRRVTSHPRADLAPTWLPTAGEPPSEP
                     AVEPAAELSAAQAAPGGSSAIRRPRGEQPVREPLAARRITQRGRRIADGVRLIRAVHR
                     GVPQRVFALRVNPRRRHAIDTALAGDRLAGRRRTRQIVRANRALAGVNGDFSLPSGSP
                     VHAFLSDGDLKRTTPTAGVQFAVSRGQRRLFLGWGAPGLRLLETSSSDRFPIARWNDG
                     SPVVGEVAGFTAAGRGVMRPPSASCAVRLRGAGPIRWTEDGSLEKAYTVERAGCFASR
                     LTRRGGVVLAAPFGTAEAVLLRSIRREEVVHVSWGFGWPRAFDSIGGTPLLVEDGRVV
                     ATSCGASFCARHPRTAVGITRTGKVLLVVVDGRRPRWSRGMTLVELAREMRRLGAVSA
                     VNLDGGGSSTMVVRDRLVNRPSDPTGERAVSSAVLLLRGRDRQPRPEEPAPLGLSSAK
                     ASLDDPASTGGLLEALERDAFATWRR"
BASE COUNT         1793 a         4642 c         4542 g         1752 t
ORIGIN      
        1 gatctggctg gccgtgcgtc tgccgatgct ggcgaccgcg aggcccgcgg ccgccgagcg
       61 gcacggcctg cgcgccgcgg cctgaggccg acggggcgcc cgcgcgggga tgactccccg
      121 cgcgggcgcc ctcacgccac cttccgctcc agctcgtcca gggagtccaa gagggcgtcc
      181 cccaggtaga cgccggggac cccctccctc ttggaaggca cggcgaacgc cgctcctgcg
      241 tagaacaccg gcaccgaggt ggctgccacg gcctccagcg cctcaagggc cgagcgccgg
      301 gctcgcgtcc actgcgagga cagcaccacc gccgcaggac ctaccttgct caccgtccgc
      361 agcaggctgt ccacggggat gcgggcgccc aggacgaccg tcggccatcc gcgccgcgcc
      421 aggagcaggg cgaaggcctc gagtccggtg gcgtgcgtct cgtccggcgc cgccgccaga
      481 aggatcggag ccgggcggaa gggcggtggc gcggtgcgac cgagcctcgc gatccaggac
      541 cggacctcgt tcgttgccag atgctcctgg gccacgtcgc agcggccccc cgcccatcga
      601 tcgcccacct ctcgcagggc gggcagggcc acctccacga cggcccgctc gatcccgagc
      661 ccgtccgcgg cttggtcgag gctcccccgg atccccgcat cgtcgagatc gaggacgcgg
      721 tcgaggagcg tctcgagatg cggggtgggc tcgatggcgg gcgcgggagc cagccggatc
      781 ctccgcgccg cgtcgcgtgc gctcgcccct cgcacgatct cgtcgcgcat ggcccggatc
      841 aggtcgacct cctcccgcgt gtatcggcgg tgtccacctc ccgtgcgagc gggcgccggt
      901 accccgtatc gacgctccca cgagcggatc gtggggatcg gcacgtcgac gaagcggctc
      961 acctccgaga tcgagaggcg ctcgggctcg gtcatgccgg cacgatacgc gagcgccccg
     1021 gaacgtgtcc aacctgcatc gacttcgggc cgggatgcag aggttcggag gggccgggag
     1081 accggcggcc ccccccgctc cctcaggccg gtgccggcgc ctccgcggac gcggggggct
     1141 ccggcccgac gccctccagc gacacgttgg ggaggatgcg gtcgagccac cccggcagcc
     1201 accagttcca gtcgcccatc aggcgcatcg tggctggaac gagcaccacc cggaccaacg
     1261 tcgcgtcgag gaagaccgcc gcggccagcc cgaaccccat cgacttgata ggcacgaggc
     1321 tggcgaaggc gaacgccccg aagaccgtca ccatgatcgc cgccgcgctc gtgatgatcc
     1381 tcgccgtgtg ctcgaggccc caaccgaccg cctcggtgtt gtccccggtg cgcaggtact
     1441 cctcgcggac gcgggccagc aggaagacct cgtagtccat cgagagcccg aacaggatcg
     1501 tgaacaggaa cagcggcacg aacgactcga tgtgcccggt ggccgagaac cccagcagct
     1561 cctggaagtg gccctcctgg aagatgaaca ccacgatgcc gtaggtcgcg agcaccgagg
     1621 ccaggttcat caggatcgcc ttcagcggca ggaggatgct gcggaagaag agcagcagca
     1681 agaagaacga gagcgtgagg accatggcca cgaccgggat gatcgcgctc tcgatctcct
     1741 cgttgagatc cacggtgagg ccgggatcac ctccgacggc cacctcggct cccgccggca
     1801 ggagcgcggg aaggtcctcg cggagacggc gcacgagctc gcggacctcg tcggaccccg
     1861 gctcgtcggc ggtgaccacc cgcagcagcg tggccttccc gtcgttcccc accatcgtct
     1921 ggatgaccgg ggccgcctct ggcgacgcgg ccaccgcgcg ggcctcctcc acgctcgcca
     1981 ccggggccag cgtcgcgatc gagtccacct gcaggatgct cgggtcgctc tgcagcgccc
     2041 cggccacctc ggccacgtcc tcgaacccgt cctcgagcac cccctcgggg tgctcgatca
     2101 ggatctgcat gggggcggcc cgtccctcgc cgaacgcctc ggccaccgcc tcggccgcca
     2161 cccgaggctc cgagtcgggc gggaagatgt cgggacccgc ggtccccaaa cgcaggttcc
     2221 gggctggcca cgccagcagg gccagcaggg ccagcgaccc gatcagggcg aaccaggggc
     2281 gccgcatgac gagcatcgcc caccggtgcc acaagccgcc cgaccgctcc tcgtccttta
     2341 ggcgccgccg acccaggcgc aggctgttga tcttcctccc cacgaaaccc agcagcgccg
     2401 ggaggatcgt gagcgctgcc aggccggcga tggacacggc gatcatcgcg ccgagcccca
     2461 tcgagcggaa cgcggcgatg tccaccagca gcgtgcccga gagcgcgatc acgacggtga
     2521 gcgccgagac catgacggct ttcccgctcg tggccatggc ccgggccacg gcctcgcgga
     2581 cgtcgtgccc ctcccccagc tcctggcggt agcggctcac gacgaacagc gagtagtcga
     2641 tgccgacccc gatcccgatc atggaggcca cgttctgggt gaagatgctc acggtcgtcg
     2701 cggcggcgac gagggagatg atcccgaacg agatcgccag gccgaacagg gcgagggcca
     2761 gcggcatccc cgccgcgacc agggacccga acgcggcgat caggatcagg aggctgatgg
     2821 gcagggcgat ggcctccgcc cgctccagat cgtgctgggt cacctcggag aactccgaat
     2881 agaagggagc cgctccggtg atgtaggcct cgatgtcgcc cccgtgcggc acggcgccgt
     2941 gcacggcgtc gtggagctcg tcggcgtgct ccagggcctc gtcctgatcg tcggtgagac
     3001 cgaccgtgga cacgagcgtc cggccgtccg ggccgatcga tcgctccggc gtcgcgtacg
     3061 ggtccgtgac gtcccccacg ccgggcgcct cgaggagggc cgccctgacg tccccgaagg
     3121 cctcccggaa ccgcgggtcg tccacagtca cgctctcgct ccgcatcacc agctggtcgc
     3181 tgaactcgaa ctgccccgtg aactcgctct gcaccgcgag ctggacccgc tccgactcgg
     3241 acccgggcac ctcgaaccct ccctggctga gccggtcccc caactcgcgc aggggcacga
     3301 acgcggccac cagcgccgcc acccagaccg ccaccaccca ccaccgccgc cggacggcga
     3361 agccgcccag tcggtggaac acggacctcc ggatctcctt catgtcactt gctccttggc
     3421 tctgatcgca tccagcacca gcccggtgag ggcctccgac caccgccgga gatcccccgc
     3481 acctctcgtc cccgagacgt gctccagccc gatgaacccg gccaccacga cctcggagac
     3541 ctcccgggga tcgaggtccg ggcggacgtc gccctcctcc tgggcccggc gcagcaggtc
     3601 ggtcgagaac tccacccagg acctgaactg cacgtcgagc accgaggcca gcctggggtc
     3661 gccagacagc tcgccggcca gccggccgag ggaccgggcc gagggatctg cctcgacgca
     3721 ctcggtgacc gcgcccatga cggccacgag ctgatcgagc gcgcgcgcgc cctcctcgat
     3781 cgcctcggcc accatcgcct gccactcctc gtgcttccag cggaagacct cgagcgccag
     3841 gtcctccttg gagtcgaagt ggaagtagaa ggcgcccttg gtcaggccgg tggctcgcac
     3901 gagctcgttc atcgacgtgc cggcgtagcc tcgctcggcg aaggccgccg cggccgcggc
     3961 caggatccgg ctgcgggtct cctcgccccg gtctgttcgc cgggccatgg cgccgaccat
     4021 acataccgac cggtcggtct gtcaagtctt ggttagtcgg gccggaggac ggatagggtg
     4081 gggcgggagg aggtgtgttc gtatgcttca ggtcaccgaa gccgccgtcg gcgtgatccg
     4141 ccagatcagg atggacaccc ccggggtgtc ggacggggcg aaggcccgta tccagctcgt
     4201 cgcctccggc gaggaggagc agcccgggct cgggttcggc ttctccgacg agccgatcga
     4261 gggcgatcac aaggtcgccg agaaggagga catccaggtg ttcctggccc ccgagctcgt
     4321 ggagccgctc tcggctgcgg tcgtggacgc cgtcccggcc gcagaaggcc agcagctcgt
     4381 gctccgcgag caggccgagg aggggtgagc ccccgccccg ctagcatggg ggcgtgccgg
     4441 agtatccaga ggtccaggcc ctcgccgagc gcctcgacga cctgttccac ggcgccgcgc
     4501 tcgagggcat cgacgcgatc cagttctcgg cgctgaagac cgtcgagccg tcgccggagt
     4561 ccctgaccgg gcgggccctc gagggcgtcg ggcgccgagg gaagtggctg ctgctcgacc
     4621 tgggcggccc gcggatcgcg ctgcacctct cccagggagg tcgcgtgatg ctcgaggacc
     4681 cgcccaagcg atccaggcct cgtggcgggg tggtgcggct ccggttcgcc gggcggcccg
     4741 ccctcatggt ccgcgagttc gggaccgagc gtcgcgctgc ttggcacgtc ctggctcccg
     4801 gcgatgaggg gccgctggcc gtcctcgggc ccgaggcggc ctcggagcgg ttcgccacgt
     4861 ggctggcgag cgccgacgac gggcgacgcg tccacacgat cctccgtgat cagcggaccg
     4921 tggccgggat cggtcgcggc tacgccgacg acatcctcca ccgggcgaag ctctcgccgt
     4981 tcgcctcgct ggcgaagctc gaccccgagg agcggggccg gctgtccgag gccgttcgcg
     5041 acgtgctgtc cgaagcgctc caggaggagc ggaagcgcgt cggcggcctg cctccgaagc
     5101 tcggcgaccg cttcgcggtt cacgggcggt ggggccggcc gtgtccgcgc tgcgggggcg
     5161 acctccgcag gatctcctac gagtcgcacg aggtgaccta ctgcccccgc tgccagacgg
     5221 gcgggaaggt gctcgccgat cggcggttct cacgtctcgt ccggtagccc ggcccttggg
     5281 aacgaggatc tccgggagta cgatcgctcc gtccgtcgga aggggaaccc gccatgacgc
     5341 acgcgatctc gacgaacggg ctcgtcaaga cctacggctc gaccgtggcg ctcgacggcc
     5401 tcgacctgga agtgccggcc ggtgaggtcc acgggttcct tgggccgaac ggagccggga
     5461 agacgaccac gatccggatc ctcctcgggc tgctccgcgc ggaccggggg accgtccggg
     5521 tcctggacgg ggacccctgg gaggacgccg tggcgctgca ccggcggatc gcgtacgtcc
     5581 ccggcgacgt cgagctgtgg cccaccctca cgggcggcga gacgatcgac ctgctggggc
     5641 gtctgcgggg aggcctgaac cccacccgcc gcgacgagct gatcgagcgg ttcgatctcg
     5701 accccacgaa gaagggccgg acctattcca aggggaaccg gcagaaggtg gccatcgtcg
     5761 ccgccctcgc ctccgactcg gagctgctcc tgctcgacga gcccaccgcc gggctcgacc
     5821 cgctcatgga ggtcgtgttc caggacgtga tccggcacat caaggccgaa ggccgcacgg
     5881 tgttcctgtc cagccacatc ctggcccagg tggagaagct cgccgaccgc atcagcatca
     5941 tccggctggg tcggatcgtc cagtccggca cgctcaccga gatgcgccac ctcacccgta
     6001 ccacgatcga ggcccacaca gcgacgccgg tcagcgggct ggagacgatc cccggcgtgc
     6061 accatctcga ggccaacgac acgcgggtcc acttcgccgt ggacggcgag catctcgacg
     6121 atgccgtgaa gcacctctcg agcttcggga tccgcgcgct caccagccac ccccccacgc
     6181 tggaggagct gatgctccgt cactacggcg acgagctgga gagcgtgggc gcgggcggcg
     6241 agtgaccagc gcggccgccg ccgagaggat tcccccggcc ggggagggca cggcgttcac
     6301 cggcacgggg aggctggccc aattcatcct ccggagggac cggatccgcc tgggggtctg
     6361 ggtgggggcc atcgccctct tggtggtcct cttcgtgtcg agcctgcccc agtactacac
     6421 ggaggcctcg cagcggcagg cccgggccga gctgatggcg aaccccggcc tccgagcgat
     6481 cgcgggtccc gggtacggtc tcgacgacta cacgttcggg gccatggtcg cccaggagtt
     6541 cctgagctgg acggcgatct tcgtggcgct gatgagcttc ctcctgatgg tccgccatac
     6601 ccgggccgag gaggagacgg ggaaggccga gctcgtccgc tcggcggcgc tcgggcgcca
     6661 cgcgcacacc gcggctccgc tggtcgttct catcggcgca aacctcgtgc tgggcgggct
     6721 gatcgccgtc gggctcgggt cgctggggat ggagggggtc tcctggccga gctcgtggct
     6781 tttcggcgcc gcgctcgcct cgatcggcct ggtgtttacc gcagtggccg cggtgacgtc
     6841 gcaagtcagc ccgggcgcgc ggggcgccgc cggcctggcc ggcttcggat tcgccgtcgc
     6901 ctacctggtc cgcgccgccg gcgacatgag cgaggtcggc ggcggcttcc tgtcatggct
     6961 ctcaccgatc ggctgggccc agcagacccg ggtctacgtg gacgaccggt ggtggcctct
     7021 gctgctctcc gtggcgctga ccgctgtgct ggtgctggtg gcgttccgcc tgtcgacgcg
     7081 ccgggacttc ggcgcggggc tggtccagcc gcgtccgggc ccggcgaggg gatcgggcct
     7141 gctctccacc ccgctcggcc tggcctggcg ccttcagcgg acgagcctca tgtggtgggg
     7201 cgtgtcgatg ttcttcttcg gggttggcta cggggccctg gccggcgagg tcgagcggta
     7261 cgtcggggag ctgtccggcg cggtgcagga gtggctcgag gggatcggcg gcgacgcgat
     7321 catcgactcg tggctcgcgg tcctggccct tctcgtcggc gtgacggtcg cggtcttcgc
     7381 ggtgctcgcg gtcctgcggc cgcggaacga ggaggcggcc gggcgggccg agccggtgct
     7441 ggcgacctcc gtgtcccgcg tcggctgggt gatgagccac ctcgccgtcg cgctcgccgg
     7501 cagcgtcgtg atcctggccc tggccggcct gggtctggga atctccgcct cggcggccct
     7561 gggggacacc tccctgatgc cacgagtcgt tggggccacc ctcgtctaca tccccgccgt
     7621 gtgggtgacg gtggggatcg gcgtcgtcct gttcgggctc gtgccccggg ccacgcggct
     7681 ggtctggttc gtgatcgcct acgccggcct gatcggcatg ttcgccgaac tcctcggcct
     7741 gcccgactgg acggtcgacc tctcgccgtt cggccacgtc ccgctggtcc cggcggtgcc
     7801 ggtccggtgg gccccgctcg ccatcctcac cgcgatcgcg gcgggcctga tcacgttggg
     7861 gctctacggc ttccgccgcc gcgacctggc cgccacttga accccgccac gcggaaacca
     7921 tcacgccctt cctccttgcc aacgagccgg gtccatcgca agatggacgg cacgggagaa
     7981 aggaggtggt cctgtcgtgc atagacgttc gacgaccccc gaggtgactg gctgctgagg
     8041 ccgtccctcc gcgggcaggg gtcacctccc cgcccgtgcg ccgggacgag agccccggcg
     8101 gaatccaacc agatcaccct catcgcgggc cccggcgtgg tcgggtcggg cgaagccgga
     8161 aggcggaagc gctcgcggtg gagcagcgaa ggtggggccg ggatcgacgg agtcgatccc
     8221 ggccccaccc tctttcccgg ccccctctca cggcatccgc ggcgagtccg ctatcatcgg
     8281 acccaacgcc gatccgagga gatggcatgc agctgacgca gaccgcagcc gagaaggtga
     8341 aggagctctt ggcccaggag gggcaccccg aggccgcgct gcgcgtggcc gcccggccgg
     8401 gcggctgctg cggctaccgc tactcgatgg cgctgaccga tcagatcgac gaggcggacc
     8461 agaccgtcga gcaccacggc atccgggtgg tcgtcgactc cgagagcgtc ccgctcctcg
     8521 acgaggccac gatcgacttt gtggactcac tggagcggag cggcttcgcc atcgagaacc
     8581 ccaaggccgg gggcggctgc acctgcggcc actgaggccg cctcagcccc tccgccccct
     8641 tcgagtcgcg acgcccccat cggtggttct ccctacggct ccgaccgcac gccaataggg
     8701 cgagacctca aggaggtcgc ccgaacgacc gatagaccca gcacgccttc gggcgtccgg
     8761 ttggctgaga gaggaggtgg gcatgtcgac ccggctgctc cgggtgacgt tcgcgctcgg
     8821 tgctctgacg gtgttcgcgg ccctctgggg cgcggagcgg aagtggtagc gcgatggtcg
     8881 gacgccccgg ctccgcctcc ccggggcgtc cgctcgctcc ccgaagggcg gcgtccgtga
     8941 ccggacggcg catcaccttc tacgtgggcc tcgtatgcat cgcgggcctc cccctcgtcg
     9001 cctggctgat ggccacgaac ggcctcagcg ccgcccggat cgccctctgg accctgtggc
     9061 cgttcaccgc cttcgtggtg gtgggtcagt tcctccccgt caggatcccc cgggaggggg
     9121 aagaggagga ggagatcacc acctcgtcga ccttctcgtt cgccatcctc ctggccaccg
     9181 gcccggcccc cgccgcgctc gccctcgccg tgggatcggt cgcctccgac ctggcccgac
     9241 ggaagcccgc atggaaggcg ggcttcaacg tcgcccagtg cacgctctcg atcctggccg
     9301 cccatctcgt gctggagacg atcggcttca cgtacctctt ccagggcggc tcggccaagg
     9361 tcgagatgca cacgctcaac ctcctggccg tgctgctggc cgcgctggcc tacttcctgg
     9421 tcaacaacac cgtgaccggg atcgccctgg ccctggccca gggcctgccc gtggggccgt
     9481 acctgagacg ggacttcggg gtccaggcga gcatcgccgc cgtgctcctc gcgctctcgc
     9541 ccgtggtggt ggtggccgcc caacacagcc tcctgatggt gcccctgctc ggtctcccga
     9601 tggccgccgt ctacaagggg gcgcgcgtgt ggctggagaa ggagtcccag gagcaccagg
     9661 cgaagcacga ccctcttacc gggctcccca accgctcgct cttctaccag cgggtgcggc
     9721 gcgagatcgg gcggcgggcc aagaagccgg gctccttcgg cgtgatgatc atcgacctcg
     9781 accggttcaa ggagatcaac gacacgctcg ggcactacgc gggcgatcag ctcctgcggc
     9841 agatcgggcc ccggatgcgc gacgtcctca gggacgagga cgtgatcgcc cgactgggcg
     9901 gcgacgagtt cgccctgctg gtgccggacc tgccgggcga gatggccgcc ctccaggtgg
     9961 ccgagcggct ccaggaggcc ctggagacgc cgttcaccgt cgaggggctg ctcctcgacg
    10021 tggacgccag catcgggatc gcgatctacc ccgggcacgg gatgagcctc gacgacctgg
    10081 tgcggcgggc cgacgtggcg atgtacctgg ccaaggagac gcactcgggc ttcgagctct
    10141 acgcgcccga gcgcgaccag cacagcctgg ggcggctgac gctgctgggc gagctgcggc
    10201 gggcgatcga acgggaccag ctgatcctct acttccagcc caaggccgac atgcggacgg
    10261 ggcgtgtggt cgcggccgag gccctcgtgc gctgggacca ccccagccac ggactgctga
    10321 tccccgagaa gttcgtcccc ctcgccgagc gaacgggcat gatgcgcgcg ctcacgctgt
    10381 tcgtgctcaa ctcggcgctc cgccagtgcc gcgagtggca cgcggtgggc cagccgctcg
    10441 gcgtggcggt gaacctctcg gcccgcaacc tcctcgatct ccgcttcccc gacgaggtgc
    10501 agaagctgct ggcgagctgg caggtcgcgc cgggatggct cgagctcgag atcaccgaga
    10561 gcgcggtcat ggccgacccg gcccgagcga tgcacgtgct ggcccggctc agcgagatgg
    10621 gcgtgggggt ctcactcgac gacttcggga ccggctactc gtcgctcgtc tacctgaaac
    10681 gcctgccggt gaacgaggtg aagatcgaca agaccttcat cacccacatg tccgatgccg
    10741 aggacgacgc cgtgatcgtc gaatcgacga tcgagctggc gcggagcctg gggctgcggg
    10801 tcgtggccga gggcgtcgag accgaggacg tgtggcggcg gctcagcggc ctcggatgcg
    10861 acatggcgca ggggttctat ctgagccggc ccatgccgcc ccccgacatg gggaactggc
    10921 tccagggcgc ccagccgagc ggctggggcc tcagcgtcgc cacgtagcga aggcgtcccg
    10981 ctccagggct tccagcaggc cgccggtgga ggcgggatcg tcgagggagg ccttcgccga
    11041 ggagagcccg agcggcgccg gttcctccgg cctgggctgg cggtccctcc ctcgcaggag
    11101 gagcacggcg gacgacaccg cgcgctcgcc ggtcgggtcg gagggtcggt tcaccagcct
    11161 gtcgcgaacg accatcgttg acgagccccc gccgtcgagg ttcacggccg acaccgcccc
    11221 caggcgccgc atctccctcg ccagctccac gagcgtcatc ccccgggacc agcgcggccg
    11281 gcggccgtcc accaccacga gcaggacctt gcccgtgcgc gtgatcccca cagccgtccg
    11341 gggatgacgg gcgcagaacg aggcgccgca gctcgtggcc acgactcgcc cgtcctcgac
    11401 gagcaggggc gtgcccccga tcgagtcgaa cgcgcggggc cagccgaacc cccacgagac
    11461 gtggacgacc tcctccctcc ggatcgagcg gaggaggacg gcctccgccg tgccgaaggg
    11521 cgcggccagc accacgccgc cacggcgcgt gaggcgggag gcgaagcagc ccgctcgctc
    11581 caccgtgtag gccttctcga gcgatccgtc ctcggtccac cggatcgggc cggcgccgcg
    11641 caggcgaacc gcgcaggaag cggacggcgg tcgcatgacg ccccgcccgg ccgcggtgaa
    11701 cccggccacc tcgcccacca cgggcgaccc gtcgttccag cgggcgatcg ggaaccggtc
    11761 gctggaggag gtctccagga gacggaggcc gggcgcgccc catccgagga agaggcgccg
    11821 ctggcctctg gagacggcga actggacgcc cgcggtcggg gtggtcctct tgaggtcgcc
    11881 gtcggagagg aaggcatgga ccggcgaccc gctggggagc gagaagtccc cgttgacgcc
    11941 ggccagggcc cggttggccc ggacgatctg acgcgtgcgc cggcggccgg cgagccgatc
    12001 cccggcgagg gcggtgtcga tcgcgtggcg tcgcctgggg ttgacgcgga gggcgaacac
    12061 ccgctggggc acgcctcgat ggacggcgcg gatcaggcgg acgccgtcgg cgatccgccg
    12121 gccgcgctgc gtgatgcggc gggcggccag gggctcccgg accggctgct ccccgcgcgg
    12181 ccggcggatc gccgacgaac cgccgggggc cgcctgggcc gcggagagct cggccgctgg
    12241 ctcgaccgcg ggctcggacg ggggctcccc ggcggtgggg agccacgtcg gggcgaggtc
    12301 ggcccgggga tggctcgtca cgcgccgcac gtcgtcgccg gccgcgtcca tcgtgtacag
    12361 gtcgcggccg ccagaggccg gggcgcggac gaaggcgatc cgggccccgt cgggcgacca
    12421 agcgggcgag gcctcgtgag cgggagacgc ggtcaggttg ctctccacgc ccccctccac
    12481 gtcgaccgtg tagatctccg acgacccgtt ggggcagcag cgctcgaacg cgacgcgcga
    12541 gccgtccggc gaccacgcgg gtgacgcctc gagcggatcg cccgaggtga gccgccggag
    12601 gccggcgccg tcggcatcca cgacgaacag gtccggcgtc cgtcccggga gggcccgcga
    12661 gaacgcgacg cgcgagccgt ccggcgacca cgcggggccg gcgtcgtggg cggccggcgg
    12721 cggctccgt
//
LOCUS       DASUTZ010000032        14180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776 k141_8202,
            whole genome shotgun sequence.
ACCESSION   DASUTZ010000032 DASUTZ010000000
VERSION     DASUTZ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14180
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8202"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VF097_01455"
     CDS             <1..351
                     /locus_tag="VF097_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261493.1"
                     /translation="VEMGAASVVLVVEAGTTEGTYRFVCPRCEDTVEKRADRKVVMLL
                     LSAGVEVREVSHEVVSMDHPSVRGHQPTFEEALREDVRVAHDDPAFTLDDLIDFHFLL
                     QQDDWFEELARSAG"
     gene            373..651
                     /locus_tag="VF097_01460"
     CDS             373..651
                     /locus_tag="VF097_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261494.1"
                     /translation="MIPLAFVIAVALATLLVLVVAVVSLVRQMKRVVTSIVELQEELQ
                     PILMEIQGGADRASRRLVELQREGTAGGSRGRPHPVVPVDPDDPLAPG"
     gene            664..942
                     /locus_tag="VF097_01465"
     CDS             664..942
                     /locus_tag="VF097_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010886937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX6261495.1"
                     /translation="MPNLGFGEIIVILLVALLVFGPRRLPEIGRTVGKSLREFRRATS
                     SLKAEFEQDLEPPRRAPARRRRPPISDGQTSETAPQEEPEPETGGPPE"
     gene            1018..1749
                     /gene="tatC"
                     /locus_tag="VF097_01470"
     CDS             1018..1749
                     /gene="tatC"
                     /locus_tag="VF097_01470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX6261496.1"
                     /translation="MSVVEHLEELRTRIFVALVAILVMSVIGFVFYQDILEVILRPYR
                     RALAELPPGSRPEGALQGRLVYSSPTDPFITFLKVGFFSGLMFALPLILYQVWAFVTP
                     GLTKRERRLGAPFVLLSVALFAGGVVFAYAIIPRGLRFLLGFGGESLVPLLTVDRYVG
                     FIMMVALAFGLSFEFPLLMIFLAAARVITSDQMRTWRRYVYLGLVVFAALVTPTQDPY
                     TMLLMTVPLILFYEAAILVARFFKR"
     gene            1759..2514
                     /locus_tag="VF097_01475"
     CDS             1759..2514
                     /locus_tag="VF097_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261497.1"
                     /translation="MAIKGKKRSRGGRSRARAVAPRPTLVVPKKPFFQRTWVRWSLIG
                     LLVLAVTGLAFGVIAALRADAERRAAQEEVERVGDELERAVAPVAQVSGQSLVVLPQL
                     STTLLEIQAGEARERRVRRQITRWEEDLAVARDDLGAIETDRRDLRRSIERISGGLEF
                     FEVLLQGVPALLDEEREARVAEAIVALQGDLQTAAGTVQSGYLIYLNERVDVELDEGT
                     PGLQTQLPGSNPFGVPGPGEVPVPEAPVPEPTG"
     gene            2519..3400
                     /locus_tag="VF097_01480"
     CDS             2519..3400
                     /locus_tag="VF097_01480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX6261498.1"
                     /translation="MIVLVANPSSGGGKGRRMMPRAIQALGSLGRAYRVVVTEDGSHP
                     QRAAREAVDAAAEAVVALGGDGLVSACANALVGTGVPLAIVPTGTGNDFARRLGLDHR
                     RPLRSIGLLERGAQRAVDTVRVEGAGWTRSYVCVAGAGFDSETNQVANRMRHLTGTLK
                     YVVALMRTLRTFEPAEFRVVTDDEDLRLHAMMVAVANAASYGGGMMVCPDAEMDDGLL
                     DLCVVGAMSRRSFVAAFPSVYRGTHVTHRDVTMLRSRKVELEASRPFDVYADGERFGP
                     LPVTLTVEPRTLEVIAP"
     gene            3397..4122
                     /locus_tag="VF097_01485"
     CDS             3397..4122
                     /locus_tag="VF097_01485"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6261499.1"
                     /translation="MTDLVLIHAFPVDAAMWQRQVADLAGEARTLAPHLPGFGGEDLP
                     SGPLTVSRMADAVAAAMDRVGIDRAVIGGCSMGGYAAFEMWRRHRDRVAGLLLVDTRA
                     EPDDEEGRAKRRDLAERVREGGSGVMVDSPPPLLSAGASDALWEGVKGWIARQPGEAI
                     ALASLGMAERPDSRPDLPGINVPTTVVVGSEDTLTPPAMSEAMAAAIPDAELVVLDGA
                     GHLSNLEDPEGFLEAARGLLRRT"
     gene            4149..6797
                     /locus_tag="VF097_01490"
     CDS             4149..6797
                     /locus_tag="VF097_01490"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX6261500.1"
                     /translation="MDPERFASRYPFPLDDYQRRSIEALALGESVLVAAPTGSGKTVV
                     AEYAIEQALSRGRKAFYTTPLKALSNQKYGDFVARYGASEVGLLTGDNSINSEASIVV
                     MTTEILRNMLYQQSSTLRDLEVVVMDEVHYLQDPYRGPVWEEILIHLDLVVSVVALSA
                     TVSNAEEFGEWLTTLRGATRVIVEERRPVPLEHLYMTGRELHPMHVEEDGHLSPNPYL
                     VSLSQRELRVKEYHRRDGRRYYERRGRPREGHRRVYVPRREEVIRVLEREGILPAIYF
                     VFSRAGCDRSVEYAMEAGIRLTSAEEQERIREFVEMRTAWMDEEDLQTLGFYELREAM
                     TAGVAAHHAGMLPVFKETVEELFARGLVKIVFATETLSLGINMPAKTVVIEDLFKFTG
                     ERHEVLTPGEYTQLTGRAGRRGIDDLGYAVVLHQRQVEFERVAGLASQRTYELTSSFR
                     PSYNMTVNLVRNYTLEEARHLLNSSFGQFQADRAVVALERQAERDRAGLSGYSERLAC
                     DRGDFEEYWGMLEEAQELRREADRTEERRRQEEIKNALGRLRPGDVIFVPRARRRGLA
                     VVLANREGRPSVLTQDRKAFRATPRDFQDPPESLARVQLPKRGSARSARYRRDVSAKL
                     VSMNVRPPKRRRGGPQREALSRAEELEGRARKHPVASCPDLEEHERWARRASKLRRRV
                     RGLERRIRARTDSLARQLDRVLEVLTSLGYVSDFSLTPKGERLTRIYGEGDILVAEAI
                     EDGVLDGLTPAEVAALVSTVVYESRERTPRSPTLPTVEVEARFERLRSLYLEVRRAEE
                     RAGVELCRELDAGFAEPAFHWAEGKALEDALRETEMAPGDFVRTCKQLIDLLRMIEDV
                     ADEDLASRVHEARTAVNRGVVAYTGL"
     gene            6800..7714
                     /locus_tag="VF097_01495"
     CDS             6800..7714
                     /locus_tag="VF097_01495"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX6261501.1"
                     /translation="MPSPFGPLVLIVNPRAGRGEVGREMPELERRIVAGRLEYRIEET
                     QKPGDASRLAREAVESGERFVVAVGDDATLNEVVNGLMDGDGPIAEDLVVGAMAGGSG
                     SDFVRTFGLPDQVTTGARHLLGEHTYPIDVARITYGAEGRSRYFVNAAQVGLGGLAVR
                     RAAALPEWLGRGRQFLGFWRSVIGYRVSNLRVRADKREFEERAHSVVIANGQFLNGGM
                     RVSPKSYPGDGRLDVQVSIGPKSQAFTILPSIYRGEHLPDPQIKEMLGAEIVVESDRP
                     IPVEADGIPLGETPVRVTVLKEALRLKI"
     gene            complement(7711..10116)
                     /locus_tag="VF097_01500"
     CDS             complement(7711..10116)
                     /locus_tag="VF097_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261502.1"
                     /translation="MAWKQQRLGPRLVEAARSGDRYRDVHLVLGGTGAVGGTAVLQLL
                     SLYEEMFSVAPPGPDDVPVLVATGVTGDEISAFTRRLFRFVESRHGAEALPERVRRGY
                     LTHSGVFVALERFMVTVLPALRRLRQVPPEERPGVIEELLAELGTTQEGAADAIAETV
                     SQARPFSSFLRAYRDEHLDGDRFRSVVVGIPIPSLIAYHQQELDEAADHLRGLTPDRL
                     ENLKQRFIAALRDDLVEVQASLADEVLIAHTTGVGGMYDPRSDGGSQIRLGFAHAALD
                     RALAEKARFAQELTDLYSQAGIKVLVTAAAIGIDEVKVRERVPLHRGVRHRLFEAPVE
                     LFPGAKASQPRESKASRRAGRPVPAAQHMRVFPPITVPFDDPPSEPARFEPGDELAPT
                     YTIRSGENGFFSVADAESLYRVMKVASASELGLLLATVGLLGDDPSRPWFEGNVCYYG
                     ETDNSRQVFDFLSQPTLRRTQLSGLEPMSLQDLGSAKHQGELHTLALLILLHRLRTLD
                     VDAIDPYVDLERFDPRAFFEQRSRTLTFEQLALWEPAELLRDMQSLAAAESPDDLLPL
                     APHREHELFPRRAEAVRRVLAEALKAVWTGPSLGLPILYERDGASYLRTGYYVAPLDL
                     LVTDADAVAVWMRKAHAESGNPAPFEDFRDYHLAVGGFVDVRPHAIVCSAKSDREDLT
                     GRVARFTDEQGLRDHLRTLQPYSFFSTCGMVAVLHRLRALYGLLRESMLELGTLHEWR
                     WSMPRDAAGHMIVLPGAVEALRMVAEGLEKSTGAERLDGPWGYERRPVPDRRGTIPGV
                     HDR"
     gene            10178..11278
                     /locus_tag="VF097_01505"
     CDS             10178..11278
                     /locus_tag="VF097_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005481973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX6261503.1"
                     /translation="MGREHTERLRRAAQATAEAGLHALLVTPSADLVYLTGYDPPPLE
                     RLTCLVIRPERDPVLVVPGLEQPLAEQRLARHVPEIRSWGETEDPYALVAKVVGSKAT
                     KLACSERMWASHLLGIQRAVPKARATTASRVLGPLRAIKDRHELHMLARAARATDEAF
                     RRLMEERIEGTTEREVAGRLGDLMLELGLERVEFAIVGSGPNAASPHHDPVDRSLRAG
                     DAVVMDFGGTSGGYCSDMTRTVAVGKAPPRLREVHEVVSEAQEEALRAVYPGVPAEDV
                     DRAARQVIERAGYGDRFIHRTGHGIGLEAHEDPYIVTGNREELRPGMCFSIEPGIYLE
                     GDLGVRIEDIVVVTEEGARRLNHAPRELAEVR"
     gene            11339..12520
                     /locus_tag="VF097_01510"
     CDS             11339..12520
                     /locus_tag="VF097_01510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6261504.1"
                     /translation="MALLNIPEELLELRSTLRRFVEREIGPVEEAFAQQIQEGDYSFI
                     KDERAKLRKRSAELGFWGIHMPEEVGGMDLPFLGQVLLAEEAVRSGLVLAHAETILPI
                     VSGPSRVHLDCTPEQREKYLAPLMTADKLTCFALTEPEAGSDATRIRTRADRTDGGWV
                     LNGRKHFISAGDSADFALVFAVTDQEKGARGGITAFLVDADVPGYSVTRVQRTMSPLQ
                     NPVELTFEDCQVSDEAVLGEVGAGFYSAVKGINGARFGIAATALGVAGHLLERMREYA
                     GSRIAFDRPIGANQYVQGMVVDSYAELEQARLLVYKLAADVDDGADGRGEGALAKLAA
                     TEAVGRIADRAIQVHGGQGYMTELGLEVWYRDVRAMRLYEGTSEILRQNVAKTLGLPV
                     L"
     gene            complement(12517..12963)
                     /locus_tag="VF097_01515"
     CDS             complement(12517..12963)
                     /locus_tag="VF097_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261505.1"
                     /translation="MSSEENGQPRWRPGIPTVLVVASILVALVAVSALLYVLFRETEG
                     PGEVLRDFAEALAEDDCPGSYDLLDPSVRASITEDEWCDEVPGLAAKLSPEFEIERVI
                     LSEGDARVEVSGPETTTGAWFLRRQGRSWAVLGGGDAIEFPTELPT"
     gene            complement(12960..13760)
                     /locus_tag="VF097_01520"
     CDS             complement(12960..13760)
                     /locus_tag="VF097_01520"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6261506.1"
                     /translation="MASIFVKALRDRRRVLIWWALGMIATALMLASFYPTIRETGEDL
                     NAYIENLPEALRAIVLSGGGDFTSGPGYLNSELFSFMLPLIVLVFSVGMGAAVVAGEE
                     REGRLELMVSSPVTRRRIVLQKLAAILVGMIGLGIVLWATLAVGVEVWDIPVDHANLV
                     AACAMAVFLGWVFGALALAVGAATGRRGMAIGIPAAVGVATYLVNGLAPLSDALEFWR
                     PLSPFYYYAADQPIRSGIDLGDAGILLALTVILGVAAVLAFDRRDLAT"
     gene            complement(13760..>14180)
                     /locus_tag="VF097_01525"
     CDS             complement(13760..>14180)
                     /locus_tag="VF097_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6261507.1"
                     /translation="LGLDPLIQQHVYRLLAETKADGRTVFLSSHNLPEVERTCDRVGI
                     IRRGRLVAVEDVREIRSRALREMEIHFAAPPTGNGFSRLPGVRAVHVSGESVRMQVEG
                     EVDAVVKEAAKHRVVSITTRQPTLEEIFLAFYGEEEA"
BASE COUNT         2068 a         4946 c         5154 g         2012 t
ORIGIN      
        1 gtggagatgg gcgccgcctc cgtcgtcctc gtcgtcgagg ccgggaccac cgagggaacc
       61 taccggttcg tctgcccgcg ctgcgaggac accgtcgaga agcgcgccga ccgcaaggtg
      121 gtcatgctgc tgctctcggc cggggtggag gtgcgggagg tctctcacga ggtcgtgagc
      181 atggaccacc cttccgtccg cggtcaccag ccaaccttcg aggaggctct gcgggaggac
      241 gtccgcgtgg cccacgatga ccccgccttc acgctcgacg acctgatcga cttccacttc
      301 ctcctgcagc aggacgactg gttcgaggag ctcgcgcgat ccgcgggctg agcccctgct
      361 atcctgaccc gcgtgatccc gctggcgttc gtgatcgccg tggcgctcgc gaccctgctc
      421 gtgctcgtgg tcgccgtcgt ctccctcgtc aggcagatga agcgggtggt gacgtcgatc
      481 gtcgagctcc aggaggagct ccagccgatc ctgatggaga tccagggtgg ggccgaccgc
      541 gcgagccggc gcctcgtgga gctgcagagg gaaggcacgg ccggcggctc gcgcggtcgg
      601 ccccaccctg tggtccccgt ggacccggac gacccactgg cgccgggata agatagatcc
      661 gagatgccca acctcggctt cggagagatc atcgtcatcc tgctcgtggc cctgctcgtg
      721 ttcgggccgc gcagactgcc cgagatcggg cgcacggtgg ggaagtcgct ccgggagttc
      781 cgtcgggcga cctccagcct caaggccgag ttcgagcagg acctggagcc gccccgtcgc
      841 gcgccggcgc ggcggcggcg tcctccgatc tcagacggcc agacctccga gaccgccccc
      901 caggaggagc cggagccgga gacgggtgga ccgcccgagt aggcgggcgg cgctcccgga
      961 gcgagcttcg ccatgcgcct gatcccacgc cggcggcggc gcgatcccga aggatcgatg
     1021 tcggtcgtcg agcacctcga ggagctgcgc accaggatct tcgtggcgct cgtggcgatc
     1081 ctcgtgatgt cggtaatcgg gttcgtcttc taccaggaca tcctcgaggt catcctgcgt
     1141 ccgtatcgtc gggccctagc cgagctcccg ccgggatctc gcccggaggg ggctctccag
     1201 ggaaggctcg tctactcgag ccccaccgat cccttcataa cgttcctcaa ggtggggttc
     1261 ttcagcggcc tcatgttcgc gctgccgctg atcctgtacc aggtgtgggc cttcgtgacg
     1321 ccggggctga cgaagcgcga gcgacgcctc ggggcgccgt tcgtcctcct ctccgtggct
     1381 ctgttcgcgg gcggggtcgt cttcgcctac gcgatcatcc ctcgaggcct gcggttcctg
     1441 ctcggcttcg gaggtgagag cctcgtcccg cttctcacgg tggatcggta cgtgggcttc
     1501 atcatgatgg tggccctcgc cttcgggctg tccttcgaat tccccttgct gatgatcttc
     1561 ctggcggcgg ccagggtcat cacgagcgat cagatgcgga cgtggcgccg ctacgtgtac
     1621 ctcgggctgg tggtcttcgc agcgctggtg acgcccacgc aggaccccta cacgatgctc
     1681 ctcatgaccg tcccgttgat actcttctac gaggccgcca tcctggtggc caggttcttc
     1741 aagcgctgag gtcggttgat ggcgatcaag ggcaagaagc gctcgagagg aggccgatcg
     1801 agggcgaggg ccgtcgcgcc caggcccacg ctcgtcgtcc ccaagaagcc gttcttccag
     1861 cgcacgtggg tgcgctggag cttgatcggc ctgctcgtgc tggccgtcac ggggctggcc
     1921 ttcggggtca ttgcggcgct ccgggcggac gccgagcgcc gggccgcaca ggaagaggtg
     1981 gagcgggtgg gcgatgagct cgagcgcgcc gtggcccccg tggcccaggt ctccgggcag
     2041 tccctcgtcg tcctcccgca gctgagcacg acgctgctcg agatccaggc gggggaggcg
     2101 cgggagcgcc gggtgaggcg ccagatcacc cgctgggagg aggacctcgc tgtcgccagg
     2161 gacgacctcg gggcgatcga gaccgaccgc cgcgacctcc gcaggtcgat cgagcggatc
     2221 tcaggcggcc tggagttctt cgaggtgctc ctccaggggg tccccgcgct cctcgacgag
     2281 gagcgcgagg cccgggtggc cgaggcgatc gtcgccctcc agggcgatct ccagaccgcg
     2341 gctgggaccg tccagagcgg ctacctgatc tacctcaacg agcgggtgga cgtggagctg
     2401 gacgagggca cgcccgggct ccagacccag ctcccgggga gcaacccgtt cggcgtcccc
     2461 gggccgggag aggtccccgt tcccgaggcc ccggtccccg agcccaccgg gtgacgccgt
     2521 gatcgtcctg gtggccaacc cctcgtccgg cggggggaag ggccgccgga tgatgccccg
     2581 cgcgatccag gccctcggct cgctgggacg tgcgtaccgc gtcgtcgtca ccgaggacgg
     2641 gagccacccc cagcgcgcgg cccgcgaggc cgtggacgcg gcggcggagg cggtcgtggc
     2701 cctgggcggg gacgggctcg tgagcgcgtg cgccaacgcg ctggtcggca ccggcgtccc
     2761 cctggccatc gtgcccaccg gcaccggcaa tgacttcgcc cgccgcctcg gcctggatca
     2821 ccggcgcccc ctccgctcga tcggcctcct ggagcgcgga gcccagcgcg cggtggatac
     2881 cgtccgcgtg gagggcgcgg gctggacgcg atcctacgtg tgcgtggcgg gagcgggctt
     2941 cgactccgag acgaaccagg tggccaaccg gatgcgccat ctcaccggga cgctgaagta
     3001 cgtcgtggcc ctgatgcgga ccctgcggac cttcgagccg gccgagttcc gggtggtcac
     3061 cgacgacgag gacctccgcc tccacgccat gatggtcgcc gtggccaacg ccgcctccta
     3121 cgggggaggg atgatggtct gccccgacgc cgagatggac gacggtctcc tcgatctgtg
     3181 cgtggtcggg gcgatgtcga ggaggagctt cgtggccgcg ttccccagcg tctaccgggg
     3241 cacccacgtc acccaccgcg acgtgacgat gctgcggtct cgcaaggtcg agctcgaggc
     3301 ctcgcgcccc ttcgacgtgt acgcggacgg ggagcgcttc ggaccgctcc ccgtgaccct
     3361 caccgtggag ccccgaaccc tggaggtgat cgccccgtga ccgacctcgt cctgatccat
     3421 gccttccccg tggacgccgc gatgtggcag cggcaggtcg cggacctcgc cggcgaagct
     3481 cggacgctcg ctccccacct gcccggcttc gggggggagg acctcccgag cggtccgctg
     3541 acggtgtccc ggatggccga cgccgttgca gccgccatgg accgggtcgg gatcgaccgt
     3601 gcggtgatcg gtgggtgctc gatgggtggc tacgcggcgt tcgagatgtg gcgacgacat
     3661 cgggatcggg tggccggcct gctcctcgtg gacacgcggg cggagcccga cgacgaggag
     3721 gggcgcgcca agcgccgcga cctggccgag cgggtccggg aaggggggag cggggtcatg
     3781 gtcgatagcc cgccgccact gctgtccgcg ggggcctccg acgccctctg ggaaggggtc
     3841 aaggggtgga tcgcgcgcca gccgggggaa gcgatcgccc tcgcctcgct gggcatggcc
     3901 gagcggccgg actcccgacc ggacctgccc gggatcaatg tccccaccac cgtcgtggtc
     3961 ggctccgagg acaccctcac gccgcccgcg atgtccgagg cgatggcggc ggcgatcccg
     4021 gacgccgagc tggtcgtgct cgatggagcc gggcaccttt cgaacctgga ggaccccgaa
     4081 gggttcctgg aggccgcgcg gggcctcctt cgccgcacct gacgggatcg cgtgggagga
     4141 tgggggcgat ggaccccgaa cgcttcgcct cccgctatcc cttcccactc gacgactacc
     4201 agcgccggtc gatcgaggcg ctggccctcg gcgagtcggt gctcgtggcg gcccccacgg
     4261 ggtcggggaa gacggtcgtg gccgagtacg cgatcgagca ggccctctcc cgcggccgga
     4321 aggccttcta caccacgccg ctgaaggccc tctccaacca gaagtacggg gacttcgtgg
     4381 cgaggtacgg ggcctccgag gtggggctcc tcaccggcga caactcgatc aactccgagg
     4441 cctcgatcgt cgtgatgacc accgagatcc tccggaacat gctgtaccag cagagctcca
     4501 ccctccgcga cctcgaggtc gtcgtgatgg acgaggtcca ctacctgcag gacccgtacc
     4561 ggggcccggt ctgggaggag atcctgatcc acctcgacct cgtagtctcc gtggtggccc
     4621 tttcggcgac ggtctccaac gccgaggagt tcggcgagtg gctcaccacc ctgcgcggtg
     4681 cgacccgggt gatcgtggag gagcgccggc cggtcccgct cgagcacctc tacatgaccg
     4741 ggcgagagct ccacccgatg cacgtcgagg aggacggcca cctctcaccc aacccgtacc
     4801 tcgtctcgct ctcccagcgc gagctccggg tgaaggagta ccacagacgc gacggtcgcc
     4861 ggtactacga acggcggggc cgacccaggg aggggcatcg ccgcgtctac gtgccgcggc
     4921 gcgaggaggt gatccgggtc ctggagcgcg agggcatcct cccggcgatc tacttcgtct
     4981 tctcccgcgc cggctgcgac cgcagcgtcg agtacgcgat ggaggccggg atccgcctca
     5041 cgagcgcgga agagcaggag cgcatccggg agttcgtgga gatgcggacc gcgtggatgg
     5101 acgaggagga cctccagacc ctcgggttct acgagctgcg cgaggcgatg accgccgggg
     5161 tcgccgccca ccacgccggg atgctgccgg tcttcaagga gacggtggag gagctgttcg
     5221 cccgggggct ggtcaagatc gtcttcgcca cggagacgct ctcgctcggc atcaacatgc
     5281 cggccaagac ggtggtgatc gaggacctct tcaagttcac gggggagcgc cacgaggtcc
     5341 tgacgcccgg cgaatacacg cagctcacgg ggcgcgcggg ccggcggggc atcgacgacc
     5401 tgggctacgc ggtcgtcctc caccagcgac aggtcgagtt cgagcgggtg gcggggctgg
     5461 ccagccagcg cacctacgag ctcacctcct cgttccgccc ctcctacaac atgaccgtga
     5521 acctcgtccg caactacacg ctggaggagg cgcgtcacct tctgaactcc tcgttcggcc
     5581 agttccaggc cgaccgggcg gtggtggcgc tggagcgcca ggccgagcgc gaccgggcgg
     5641 gcctctccgg atactcggag cggctggcct gcgaccgcgg cgacttcgag gagtactggg
     5701 ggatgctgga ggaggcccag gagctccgcc gggaggccga ccggacggag gagcgccggc
     5761 gccaggagga gatcaagaac gctctcggga ggctccggcc cggtgacgtg atcttcgtgc
     5821 ccagggcccg caggcgaggc ctggccgtcg tgctggcgaa ccgcgagggg cggccctcgg
     5881 tcctcaccca ggatcggaag gcgttccgcg ccacgccccg tgacttccag gacccgcccg
     5941 aatccctggc tcgcgttcag ctgccgaagc gggggtctgc acggagcgcc cgctaccggc
     6001 gcgacgtgtc cgcgaagctc gtgtccatga acgtccggcc tccgaagcga cgccgcggag
     6061 gtccccagcg tgaggccctg agccgagccg aggagcttga gggccgggct cggaagcatc
     6121 cggtggcctc gtgcccggac ctcgaagagc acgagcggtg ggcgcggcgc gcctccaagc
     6181 tgcgccgccg ggtgcggggc ctggagcggc ggatccgggc caggaccgac tcgctcgccc
     6241 ggcagctcga cagggtgctg gaggtcctaa cgtcgctggg ctacgtctcg gacttctcct
     6301 tgaccccgaa gggggagcgg ctcacgcgga tctacgggga aggcgacatc ctggtggccg
     6361 aggcgatcga ggacggcgtc ctcgacggcc tgaccccggc ggaggtcgcc gccctcgtct
     6421 ccaccgtggt ctacgagtcc cgggagcgga cgccccggtc gcccacgctt cccacggtcg
     6481 aggtcgaggc gcggttcgag cggctccggt cgctgtacct cgaggtccgg agggccgagg
     6541 agcgggccgg cgtcgagctg tgccgggagc tcgacgcggg gttcgccgag cccgcgttcc
     6601 actgggccga ggggaaggcg ctggaggacg cgctccgaga gaccgagatg gcgccgggcg
     6661 acttcgtccg cacctgcaag cagctgatcg acctcctccg catgatcgag gacgtggcag
     6721 acgaggacct cgcgtctcgc gtccacgagg cgcggacggc cgtgaaccgc ggcgtcgtgg
     6781 cgtacacggg cctgtaggca tgcccagccc gttcggcccc ctcgtcctga tcgtgaatcc
     6841 gcgcgccggg cgtggggagg tggggcgcga gatgcccgag ctggagcggc ggatcgtggc
     6901 gggccgcctc gagtatcgga tcgaggagac gcagaagccc ggcgacgcct cccggctggc
     6961 ccgggaggcg gtggaatcgg gggagcggtt cgtggtcgcc gtgggcgacg acgcgaccct
     7021 gaacgaggtc gtgaacgggc tcatggacgg ggacgggccg atcgccgagg acctcgtcgt
     7081 gggagcgatg gcgggggggt ctgggtcgga cttcgtgcgg accttcggcc tccccgatca
     7141 ggtgacgacc ggggcgaggc acctgctggg ggagcatacg tacccgatcg acgtggcgag
     7201 gatcacctac ggggccgagg ggagatcgcg atacttcgtc aacgcggcgc aggtcggcct
     7261 gggagggctc gccgtccggc gcgccgcggc cctgcccgag tggctcggtc gcggacgcca
     7321 gttcctcggc ttctggcggt cggtgatcgg ctaccgcgtc tcgaacctgc gggtgcgcgc
     7381 ggacaagcgg gagttcgagg agcgcgccca cagcgtcgtg atcgccaacg gtcagttcct
     7441 gaacggcggg atgcgggtct ccccgaagtc gtatccaggt gacgggaggc tcgacgtgca
     7501 ggtctcgatc ggcccgaagt cgcaggcgtt cacgatcctc cccagcatct accgggggga
     7561 gcaccttccc gatccgcaga tcaaggagat gctcggggcg gagatcgtcg tggagtcgga
     7621 tcggccgatc ccggtggagg ccgacgggat ccccctcggg gagaccccgg tcagggtgac
     7681 ggtcctgaag gaggccctcc gcctgaagat ctaacggtcg tggaccccgg ggatcgtgcc
     7741 gcggcggtcg gggaccggcc ggcgctcgta gccccagggg ccgtcgaggc gctccgcccc
     7801 cgtcgacttc tccagcccct ccgccaccat gcggagcgcc tcgacggccc ccggcaggac
     7861 gatcatgtgg ccggctgcgt cgcgcggcat gctccagcgc cactcgtgga gggtcccgag
     7921 ctcgagcatc gactccctga ggagcccgta gagtgccctg agtcgatgca ggacagccac
     7981 catcccgcag gtggagaaga acgagtaggg ctgcagcgtc cgcaggtggt cgcggaggcc
     8041 ctgctcgtcc gtgaatcgag ccacgcgtcc cgtgaggtcc tcgcggtcgc tcttggcgga
     8101 gcagacgatg gcgtggggcc ggacgtccac gaagccgccc acggcgagat ggtagtcgcg
     8161 gaagtcctcg aacggcgcgg ggttgccgct ctccgcgtga gccttccgca tccacaccgc
     8221 gaccgcgtcg gcgtcggtca cgagcaggtc gagaggggcg acgtagtagc ccgtccgcag
     8281 gtaggaggcc ccgtcccgct cgtagaggat cggtagcccg agggacggcc cggtccacac
     8341 ggctttcagg gcctccgcca ggaccctgcg caccgcctcc gcccggcgag ggaacagctc
     8401 gtgctctcgg tgcggcgcca gggggagcag gtcgtcgggg ctctccgccg cggccaggct
     8461 ctgcatgtcg cgcagcagct ccgccggctc ccacagcgcg agctgctcga acgtcagcgt
     8521 ccgagaccgc tgctcaaaga aggccctcgg gtcgaaccgc tccaggtcca cgtaggggtc
     8581 gatcgcgtcc acgtcgaggg tcctcaggcg atggaggagg atcaggaggg ccagcgtgtg
     8641 gagctcgccc tggtgcttgg ccgagcccag gtcctgtagc gacatcggct cgagccccga
     8701 gagctgggtc ctgcgcagcg tgggctggga gaggaagtcg aagacctgcc ggctgttgtc
     8761 ggtctcgccg tagtagcaga cgttcccctc gaaccacggg cgcgacgggt cgtcgccgag
     8821 cagccccacc gtggccagca gcagccccag ctcgctggcc gaggccacct tcatcacccg
     8881 gtagagggac tcggcgtcgg caacgctgaa gaagccgttc tcgccgctcc ggatcgtgta
     8941 cgtgggcgcg agctcgtcgc ccggctcgaa ccgggccggc tcgctgggcg gatcgtcgaa
     9001 cggcacggtg atcgggggga agacgcgcat gtgctgggcc gctgggacgg gccgtcccgc
     9061 cctgcggctg gccttcgact cgcgcggctg ggaggccttc gcgccgggga agagctcgac
     9121 cggggcctcg aacagccggt ggcggacccc tcggtggagc gggacccgct cgcgcacctt
     9181 cacctcgtcg atcccgatcg ccgctgcggt caccaggacc ttgatccccg cctgcgagta
     9241 caggtcggtg agctcctggg cgaagcgggc cttctcggcc agcgcccggt ccagggcggc
     9301 gtgggcgaac cccagccgga tctgggagcc tccgtcggaa cgcgggtcgt acatcccgcc
     9361 cacccccgtc gtgtgggcga tcaggacctc gtccgccagc gaggcctgga cctccacgag
     9421 atcgtcacgc agggccgcga tgaaccgctg cttgaggttc tcgagccggt cgggcgtcag
     9481 cccgcggagg tggtcggcgg cctcgtcgag ctcctgttgg tggtaggcga tgaggctggg
     9541 gatcgggatc cccacgacga cggaacggaa tcggtccccg tcaaggtgct cgtcgcggta
     9601 ggcccggagg aacgagctga agggccgggc ctgcgagacc gtctcggcga tcgcgtcggc
     9661 cgcgccctcc tgggtggtcc cgagctccgc gaggagctcc tcgatcacgc ccggacgctc
     9721 ctcgggcggc acctggcgaa ggcggcgcag ggcgggcagc accgtcacca tgaaccgctc
     9781 gagggcgacg aacacccccg agtgcgtcag gtagcctcgc cggacccgct cggggagcgc
     9841 ctcggccccg tgtctcgact ccacgaaccg gaagagccgc cgcgtgaagg cgctgatctc
     9901 gtcgccggtc acgccggtgg ccacgagcac ggggacgtcg tcgggcccgg gaggggccac
     9961 cgagaacatc tcctcgtaga gcgagagaag ctggagcacc gccgtcccgc cgacggcccc
    10021 cgtcccgccg aggaccaggt ggacgtcgcg gtaccggtcg ccggagcgcg cggcctcgac
    10081 cagccgcggg ccgagtcgct gctgcttcca cgccatgccg gggagtctat cggaccggcc
    10141 cggggcggcc cgatcggtcc gtatcatggt gggcctcatg ggacgagagc acacggagcg
    10201 gctccgtcgg gccgcccagg ccacggcgga ggccggcctc cacgccctgc tcgtcacgcc
    10261 ctcggccgac ctcgtctacc tcacgggcta cgacccgccg ccgctggagc ggctgacgtg
    10321 cctcgtgatc cgtcccgagc gcgatcccgt cctcgtcgtc cccgggctgg agcagccgct
    10381 cgccgagcag cgcctggccc gccacgtccc cgagatccgc tcctggggcg agaccgagga
    10441 cccctacgcc ctcgtagcga aggtcgtcgg gagcaaggcg acgaagctgg cctgctccga
    10501 gaggatgtgg gcctcgcacc tcctcggcat ccagcgagcg gtccccaagg ctcgcgcgac
    10561 gacggcctcg cgcgtcctcg gcccgctccg cgcgatcaag gaccggcacg agctccacat
    10621 gctggcccga gccgcacggg ccaccgacga agccttccgc cgcctcatgg aggagaggat
    10681 cgaggggacg accgagcgcg aggtggccgg gcggctgggc gatctgatgc tcgagctggg
    10741 gctggagcgg gtcgagttcg cgatcgtggg ctccggcccc aacgcggcca gcccccacca
    10801 cgacccggtt gaccggtccc ttcgagcggg ggacgcggtg gtgatggact tcggcggcac
    10861 ctccggaggc tactgctcgg acatgacccg gacggtggcg gtggggaagg cgccgccgcg
    10921 gctccgcgag gtccacgagg tcgtgtccga ggcccaggag gaggccctcc gagccgtcta
    10981 tccgggcgtc ccggccgagg acgtggatcg cgccgctcgc caggtgatcg agcgcgccgg
    11041 ctacggcgat cggttcatcc accggaccgg gcacgggatc ggcctggagg cccacgagga
    11101 cccctacatc gtcaccggga accgcgagga gctgcgaccg gggatgtgct tctcgatcga
    11161 gccggggatc tacctggagg gagacctcgg cgtcaggatc gaggacatcg tggtcgtcac
    11221 cgaggagggc gcgcgccgcc tcaaccacgc ccctcgcgag ctggcggagg ttcggtaggg
    11281 ctgcacccgt gataacctac gcctcggtag gttccgacag gcgaacggag gaggccccgt
    11341 ggccctgctc aacatccccg aggagctcct cgagctccgc agcacgctcc ggcggttcgt
    11401 cgaacgcgag atcggccccg ttgaggaagc cttcgcccag cagatccagg agggcgacta
    11461 ctcgttcatc aaggacgagc gagcgaagct ccggaagcgc tccgccgagc tcgggttctg
    11521 ggggatccac atgccggagg aggtcggcgg gatggatctg ccgttcctgg ggcaggtgct
    11581 gctggccgag gaggcggtgc gcagcgggct cgtcctggcc cacgccgaga cgatcctccc
    11641 catcgtctcc ggccccagcc gcgtgcacct cgactgcacc ccggagcagc gcgagaagta
    11701 cctggcgccg ctcatgaccg ccgacaagct cacctgcttc gcgctcaccg agcccgaggc
    11761 cggctccgac gccacccgga tccgcacgcg ggccgacagg accgacggag gctgggtcct
    11821 gaacggccgg aagcacttca tcagcgcggg ggactcggcg gacttcgccc tcgtcttcgc
    11881 cgtcacggac caggagaagg gcgcccgcgg aggcatcacg gcgttcctgg tggacgccga
    11941 cgtcccgggc tacagcgtga cccgggtcca gcgcacgatg tcgccgctgc agaaccccgt
    12001 ggagctgacg ttcgaggact gccaggtctc cgacgaggcc gtgctggggg aggtcggcgc
    12061 cggcttctac tccgccgtga agggcatcaa cggcgcccgg ttcggcatcg ccgcgacggc
    12121 cctcggggtc gcggggcacc tcctggagcg gatgagggag tacgccgggt cgaggatcgc
    12181 cttcgaccgg ccgatcggcg ccaaccagta cgtgcagggg atggtcgtgg actcctacgc
    12241 cgagctcgag caggcgaggc tcctcgtcta caagctggcg gccgacgtcg acgacggggc
    12301 cgacggccgc ggggagggag cgctggccaa gctggcggcc acggaggcgg tgggcaggat
    12361 cgccgacagg gccatccagg tccacggcgg ccaggggtac atgaccgagc tcggcctcga
    12421 ggtctggtac cgggacgtcc gggccatgcg gctgtacgag gggacgtcgg agatccttcg
    12481 gcagaacgtg gccaagaccc tggggctgcc ggtcctctag gtcggcagct cggtcgggaa
    12541 ctcgatcgcg tcgccgcccc cgagcacggc ccaggaccga ccctgccgcc gcaggaacca
    12601 cgccccggtc gtcgtctcgg ggcccgaaac ctcaacgcgc gcgtcgccct cgctgaggat
    12661 gacgcgctcg atctcgaact ccggtgacag cttcgccgcg aggccgggca cctcgtcgca
    12721 ccactcgtcc tccgtgatcg aggctcggac gctcgggtcc aggaggtcgt aggagcccgg
    12781 gcagtcgtcc tcggccagcg cctcggcgaa gtcgcgcagg acctcccccg gcccctccgt
    12841 ctcccggaag agcacgtaca gcagagcgga gacggccacc agcgcgacga ggatcgaggc
    12901 cacgaccagc acggtcggga tgcccgggcg ccagcggggc tggccgttct cttcgctgct
    12961 catgtggcca ggtcccggcg gtcgaaggcg agcacggccg cgacgcccag gatgacggtg
    13021 agggcgagga ggataccggc gtccccgagg tcgatgccgc tgcggatggg ctgatcggcc
    13081 gcgtagtagt agaagggcga gagcggccgc cagaactcga gcgcgtcgga caggggtgcc
    13141 aggccgttga cgagatacgt ggccacgccc accgcggcgg ggatcccgat cgccatgccc
    13201 cggcgccccg tggccgcgcc cacggccagc gcgagcgccc cgaacaccca ccccaggaac
    13261 accgccatcg cgcaggccgc caccaggttc gcgtgatcca ccgggatgtc ccagacctcg
    13321 acgccgacgg ccagcgtggc ccagaggacg atccccaggc cgatcatccc gacgaggatc
    13381 gcggccagct tctgcaggac gatcctgcgg cgggtgacgg ggctcgacac catgagctcg
    13441 aggcggccct cgcgctcctc tcccgccacc accgcggccc ccatccccac gctgaacacg
    13501 agcacgatca gcgggagcat gaacgagaag agctcgctgt tcaggtagcc cggccccgag
    13561 gtgaagtcgc ctccccccga cagcacgatc gcccggagcg cctccggcag gttctcgatg
    13621 taggcgttga ggtcctcccc ggtctcccgg atcgtcggat agaacgaggc gagcatcagg
    13681 gcggtggcga tcatgcccag cgcccaccag atcagcacgc gtcgccgatc gcggagcgcc
    13741 ttcacgaaga tgctcgccat cacgcctcct cctccccgta gaacgcgagg aagatctcct
    13801 cgagcgtggg ctggcgggtc gtgatcgaga ccacgcggtg cttcgcggcc tccttcacca
    13861 ccgcgtccac ctctccctcg acctgcatcc ggacggactc accggagacg tggaccgcgc
    13921 ggaccccggg gagccgcgag aacccgttgc ccgtcggcgg cgcggcgaag tggatctcca
    13981 tctcccgcag cgcccgcgag cggatctccc ggacgtcctc gaccgcgacg agccggccgc
    14041 ggcggatgat cccgacgcgg tcgcaggtcc gctcgacctc ggggaggttg tgggaggaga
    14101 ggaacacggt ccggccgtcg gctttcgtct cggccaggag ccggtagacg tgctgctgga
    14161 tcagcgggtc gaggccgagc
//
LOCUS       DASUTZ010000033         5394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1174537, whole genome shotgun sequence.
ACCESSION   DASUTZ010000033 DASUTZ010000000
VERSION     DASUTZ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174537"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..141)
                     /locus_tag="VF097_01530"
     tRNA            complement(68..141)
                     /locus_tag="VF097_01530"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(105..107),aa:Ile,seq:gat)
     gene            complement(193..603)
                     /locus_tag="VF097_01535"
     CDS             complement(193..603)
                     /locus_tag="VF097_01535"
                     /inference="COORDINATES: protein motif:HMM:NF023511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3566 domain-containing protein"
                     /protein_id="HEX6261508.1"
                     /translation="MATIQETRRGSPAAAGGPAPRRIRVAIRRVNPWSVLRFSLLFYF
                     CLMLVFVIGLAILYMVVDALGVVTSLEQLLESLGFGGDAGFEVNGGFLFRTIFLIGLV
                     SVVIWSALTLFFAFLYNLISDLVGGIEVTLAERR"
     gene            complement(606..2039)
                     /locus_tag="VF097_01540"
     CDS             complement(606..2039)
                     /locus_tag="VF097_01540"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012182691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX6261509.1"
                     /translation="MQLEPLDPFVWRVPMGTVPGMRVPGIVFATEVLARAIEGDNAIE
                     QVANVATLPGIVGASFAMPDIHWGYGFPIGGVAATDPERGGVVSPGGVGFDIACGVRL
                     LRSELTLDDVDDLRGLVAALGGRVPRGVGERGRMRLSRSEVEKVLTEGVRYPLSQGMG
                     WQEDEELCEDFGVLEDALPDEVSDRAIERGAPQLGSLGAGNHFLEVQVVEEVLDERAG
                     NVMGLFPGQVCVMLHSGSRGLGHQVCTDYLKLVDAAMRAEGIEVPDRQLACVPVQSEV
                     ARRYLGAMFAAANFARANRHVLAHLVRETFEAFLGRSAREMGLRLVHDVAHNLAKMEE
                     HEVEGERRLLCVHRKGATRAFGPGSREIPEPYRRIGQPVIIPGSMGTASYVLVGTDEP
                     TAFASTCHGAGRAMSRTQAKKRMSGKELVKQLEGEGIVMAASKPRLLAEEAPYAYKDV
                     SQVVDACEGAGLSRKVARLRPVGVVKG"
     gene            complement(2082..2495)
                     /locus_tag="VF097_01545"
     CDS             complement(2082..2495)
                     /locus_tag="VF097_01545"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HEX6261510.1"
                     /translation="MTRSHELLEHTADVGILARGDTREEAFAAAAEGLAEILGSWFPG
                     RGEEREVRIEGLDVEALLVAWLDELLYLSEAGDAVFGGFEVDRVGEREVLARARVAPR
                     AGRELEGQHVKAATYHRLRVAQEPPGWTARVFLDV"
     gene            complement(2492..2740)
                     /locus_tag="VF097_01550"
     CDS             complement(2492..2740)
                     /locus_tag="VF097_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261511.1"
                     /translation="MGVWLAAGAATATLLIGLVLRTRLPAPLGVALMVAASAALAWGG
                     VLLREDPSAFEAALATSALAIMGPLHVRIVLGPYGPPR"
     gene            complement(2742..5192)
                     /gene="gyrA"
                     /locus_tag="VF097_01555"
     CDS             complement(2742..5192)
                     /gene="gyrA"
                     /locus_tag="VF097_01555"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX6261512.1"
                     /translation="MTDGQGTIRASRIEDEMKVSYLDYAMSVIVSRALPDVRDGLKPV
                     HRRILWAMLEDGLRPDRPTRKSATVVGSVIGRYHPHGDQAVYDALVRMAQDFSLRYPL
                     VDGQGNFGDIQGNPPGAYRYTESRLDPLAMELLRDIDKETVDFLPNFDGNHQEPIVLP
                     ARFPNLLANGAAGIAVGMATNIPPHNLGEVIDAVVAAVDQPNITPAQLMKHVKGPDFP
                     TGGMIMGRDGIKDAYLAGRGSLKMRGRVVVEEDSKGRQRLVVTEVPYQVNPARLAEKI
                     QEMVNAKRLPEVPGRGVRNETSGRGGMRIVVDLNRGANPQIVLNKLYKHTQLQENFGV
                     IMLALVDGVPRVLTLAEMIRHFVEHQIEVLTRRSKYELRKAEERDHIVEGLLKALTNI
                     DEVIKIIRGAKDADDARTKLMKRFKLSEIQANHILDMPLRRLTRLERTRLEDEHEELQ
                     ATIKRLKALLKDPKKIRKLLKDELAEIREKYADERRTEIRRDDGEIDIEDLVAREDVV
                     ITVSRAGYVKRMPVTAYRRQGRGGRGVAGGKLKEEDLVAQVFTTTTRHWLLFFTNSGK
                     VYRVKAYEVPEQSRTARGIYAANLPGVSVTSGERIAAVLAIEDYDAAKYAVFATKKGM
                     IKKTALKEYDSPRTGLAAIRLRKGDELIRVRLSSGRDDVVMVSRGGKAIRFKESAVRA
                     MGRQTSGVIGMRVPPKDEVITMVLARKGEDLVTITESGLGKRTPISEYPVKGRGGQGV
                     ITHKLTAKTGKLAGAFSSMRDQDVFVISTSGIVIRVPGKDIRQTGRPSQGVRIMRLEP
                     KQKVAVAAPVIESEEQEA"
     gene            complement(5206..>5394)
                     /locus_tag="VF097_01560"
     CDS             complement(5206..>5394)
                     /locus_tag="VF097_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261513.1"
                     /translation="HRAWVVRDSLAKLDPGTADGLRALLAGIRRRPAAPSTSDAAALA
                     QRFGAMGLGRRMPEPPLT"
BASE COUNT          766 a         1952 c         1813 g          863 t
ORIGIN      
        1 gcccagagga agaacctctt cttcccgctc ttcttctcct cgcccatcgt tcctcctttc
       61 gcgtgcgtgg gcctgggtgg actcgaacca ccgacctcac ccttatcagg ggtgcgctct
      121 aaccgcctga gctacaggcc cgaggtccgg gctcagcgta cttgcgcccc ctcgaaagcg
      181 tcaatacagc ggtcagcggc gttccgcgag ggtgacctcg atgcctccga cgaggtccga
      241 gatcaggttg tagaggaatg cgaagaagag cgtgagcgcg gaccagatca cgacggagac
      301 gaggccgatc aggaagatcg tccggaagag gaaccctccg ttcacctcga agcccgcgtc
      361 gcccccgaat cccaggctct ccagtagctg ctccagcgag gtcacgacgc ccagggcgtc
      421 caccaccatg tagaggatcg ccagcccgat cacgaagacg agcatcaggc agaagtagaa
      481 gagcagcgag aacctcagca ccgaccaggg gttgacgcgc ctgatcgcca cccggatccg
      541 ccggggcgcg ggtccgcccg ccgccgcggg cgagcctcgt ctcgtctcct ggatcgtcgc
      601 catccctatc ccttcacgac gccgacggga cggaggcggg ccaccttcct ggagagaccc
      661 gcgccctcgc acgcatcgac gacctggctc acgtctttgt acgcgtaggg agcctcctcg
      721 gcaagcagcc ggggcttgga ggccgccatg acgatccctt ccccttcgag ctgtttgaca
      781 agctccttcc cggacatccg cttcttggcc tgcgtccggc tcatcgcgcg gccggccccg
      841 tggcaggtcg atgcgaaggc cgtgggctcg tcggtcccga ccaggacgta ggaggccgtc
      901 cccatgcttc cggggatgat caccggctgc ccgatccgcc tgtagggctc ggggatctcg
      961 cgactgccgg ggccgaaggc ccgcgtggcg cccttgcggt gaacgcagag gagccggcgc
     1021 tccccctcca cctcgtgctc ctccatcttg gccaggttgt gcgccacgtc gtgcacgagg
     1081 cggagcccca tctcccgcgc cgagcgcccg aggaaggcct cgaacgtctc ccggacgaga
     1141 tgggccagga cgtggcggtt ggcccgggcg aagttcgctg cggcgaacat cgcgcccagg
     1201 tagcggcggg ccacctcgct ctgcacgggg acacaggcga gctggcgatc ggggacctcg
     1261 atcccctccg cccgcatcgc cgcgtccacg agcttgaggt agtcggtgca gacctggtgg
     1321 cccaggcccc gggagcccga gtgcagcatg acgcagacct ggccggggaa gaggcccatg
     1381 acgttcccgg cgcgctcgtc gaggacctcc tccacgacct ggacctccag gaagtggttg
     1441 cccgcgccga gcgagccgag ctggggggcg ccccgctcga tcgcccgatc ggagacctcg
     1501 tccgggagcg cgtcctcgag gaccccgaag tcctcgcaca gctcctcgtc ctcctgccac
     1561 cccatcccct gggacagcgg gtagcggacg ccctcggtca gcaccttctc cacctcgctt
     1621 cgcgacaggc gcatccgtcc ccgctccccc actcctcgcg gcacccgacc tcccagcgct
     1681 gccaccagcc cgcgaagatc gtcgacgtcg tcgagcgtga gctccgagcg gagcagacgc
     1741 accccgcagg cgatgtcgaa gcccacgccg cccggcgaga ccacccctcc ccgctccgga
     1801 tcggtggcgg ccacccctcc gatggggaat ccgtagcccc agtggatgtc gggcatggcg
     1861 aaggaggcgc cgacgatccc ggggagcgtg gctacgtttg ctacctgctc gatcgcgttg
     1921 tccccttcga tggccctggc cagcacctcg gtggcgaaca cgatcccggg cacccgcatg
     1981 cccgggaccg tccccatggg gacgcgccag acgaacggat ccagcggttc gagctgcatg
     2041 gacacccgga gtctacctac cccccggttg gtccctccga atcagacgtc gaggaagacc
     2101 cgagcggtcc acccgggcgg ctcctgggcc acgcggaggc ggtggtacgt ggccgccttc
     2161 acgtgctgcc cctcgagctc ccgaccggcc cgcggggcca cccgggcccg ggctaggacc
     2221 tcgcgctccc ccacccgatc cacctcgaag ccgccgaaga ccgcgtcgcc cgcctccgag
     2281 aggtagagga gctcgtccag ccaggccacg aggagggcct cgacgtcgag cccctcgatc
     2341 cggacctcgc gctcctcgcc ccggcccggg aaccatgagc cgaggatctc cgcgagcccc
     2401 tcggcggccg cagcgaaggc ctcctcgcgg gtatcgcctc tggccaggat cccgacgtcc
     2461 gcggtgtgct cgagcagctc gtgggagcgg gtcaccgcgg cgggccgtac ggcccgagga
     2521 cgatccggac gtggagaggc cccatgatcg ccagcgcgct cgtggccagg gcggcctcga
     2581 aggcggaggg gtcctcccgg aggaggacgc cgccccaggc cagcgccgcg gaggccgcca
     2641 ccatcagggc gacgccgagg ggcgcgggga gcctggtccg gaggacgagg ccgatgagca
     2701 gggtggccgt ggccgcgccg gcggcgagcc agacgcccac gtcaggcctc ctgctcctcg
     2761 ctctcgatca ccggcgcggc cacggccacc ttctgcttcg gctcgaggcg catgatcctc
     2821 acgccctggc tcggccggcc ggtctgcctg atgtccttcc ccgggacccg gatcacgatc
     2881 ccagaggtcg agatgacgaa gacgtcctgg tccctcatcg acgagaaggc cccggccagc
     2941 ttcccggtct tcgccgtgag cttgtgcgtg atcacgccct gccccccgcg tcccttcacc
     3001 gggtactcgg agatcggcgt gcgcttgccc agcccggact cggtgatcgt cacgaggtcc
     3061 tcgcccttcc gggccagcac catcgtgatg acctcgtcct tcggcgggac ccgcatcccg
     3121 atcacgccgc tggtctggcg gcccatcgcg cgcacggcgc tctccttgaa ccggatcgcc
     3181 ttccccccgc gggagaccat cacgacgtcg tcgcgcccgc tggagagccg cacgcggatc
     3241 agctcgtccc ccttgcggag cctgatcgcg gcgaggccgg tccgcggcga gtcgtactcc
     3301 ttgagcgcgg tcttcttgat catccccttc ttcgtggcga agaccgcgta cttggcggcg
     3361 tcgtagtcct cgatcgccag gacggccgcg atccgctcgc cggacgtgac cgagaccccg
     3421 gggaggttgg ccgcgtagat acctcgggcg gtgcgggact gctccggaac ctcgtaggcc
     3481 ttcactcggt agaccttccc gctgttcgtg aagaacagga gccagtgccg ggtagtggtg
     3541 gtgaagacct gggccacgag gtcctcctcc ttcagcttcc ctccggccac gccgcgcccg
     3601 ccccggccct gcctccggta ggccgtcacc ggcatccgct tcacgtagcc ggcccgggag
     3661 accgtgatga ccacgtcctc cctggccacg aggtcctcga tgtcgatctc gccgtcgtcc
     3721 cgccggatct cggtccgtcg ctcatcggcg tacttctcgc ggatctcggc gagctcgtcc
     3781 ttgaggagct tccggatctt cttggggtcc ttcaggagcg ccttgaggcg cttgatcgtg
     3841 gcctgcaact cctcgtgctc gtcctcgagc ctggtgcgct cgagacgggt gagacgtcgc
     3901 aggggcatgt cgaggatgtg gttcgcctgg atctccgata gcttgaagcg cttcatcagc
     3961 ttcgtccgcg cgtcgtcggc gtccttggcc ccccggatga tcttgatgac ctcgtcgatg
     4021 ttcgtgaggg ccttcaggag accctccacg atgtggtccc gctcctcggc cttccggagc
     4081 tcgtacttcg agcggcgggt cagcacctcg atctggtgct cgacgaagtg gcggatcatc
     4141 tccgcgaggg tcaggaccct ggggaccccg tccacgaggg cgagcatgat caccccgaag
     4201 ttctcctgga gctgcgtgtg cttgtagagc ttgttgagga cgatctgggg gttggcgccg
     4261 cggttgaggt ccacgacgat ccgcatcccg ccccggcccg aggtctcgtt ccgcaccccg
     4321 cggccgggca cctcggggag gcgcttggcg ttaaccatct cctggatctt ctcggccagc
     4381 ctcgccgggt tcacctggta aggcacctcg gtgaccacga ggcgctggcg gcccttcgag
     4441 tcctcctcca cgaccacccg gccacgcatc ttcagggagc cgcgcccggc gaggtaggcg
     4501 tccttgatcc cgtccctccc catgatcatg ccgccggtgg ggaagtccgg tcccttgacg
     4561 tgcttcatga gctgggccgg cgtgatgttg ggctggtcca cggccgccac caccgcgtcg
     4621 atcacctcgc ccaggttgtg gggagggatg ttcgtggcca tgcccacggc gatcccagcc
     4681 gcgccgttgg ccaggaggtt ggggaaacgc gccggcagca cgatcggctc ctggtggttg
     4741 ccgtcgaagt tcgggaggaa gtcgacggtc tccttgtcga tgtcccgcag cagctccatc
     4801 gccagcggat cgaggcgcga ctccgtgtac cggtaggcgc cgggagggtt cccctggatg
     4861 tcgccgaagt ttccctgccc gtccacgagc ggataccgga gggagaagtc ctgcgccatg
     4921 cggacgagcg cgtcgtagac ggcctggtcg ccgtgggggt ggtagcggcc gatcacggat
     4981 cccacgacgg tggccgactt ccgcgtgggg cggtcggggc gcagaccgtc ctcgagcatg
     5041 gcccagagga tccggcggtg gacgggcttc aggccgtcgc ggacgtccgg gagggcgcgc
     5101 gacacgatga cgctcatcgc gtagtcgagg tacgagacct tcatctcgtc ctcgatgcgg
     5161 ctcgcgcgga tggtgccctg gccgtcggtc atggtcggtg gttcctcatg tcagtggcgg
     5221 ctcgggcatg cgccggccca ggcccatcgc gccgaagcgc tgggcgaggg cggcggcgtc
     5281 ggacgtcgag ggcgcggcgg gccggcgacg gatgccggcg aggagggcgc ggaggccgtc
     5341 cgcggtgccg gggtccagct tcgcgaggct gtcgcggacg acccaggccc ggtg
//
LOCUS       DASUTZ010000034         4079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1044865, whole genome shotgun sequence.
ACCESSION   DASUTZ010000034 DASUTZ010000000
VERSION     DASUTZ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044865"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..518)
                     /locus_tag="VF097_01565"
     CDS             complement(12..518)
                     /locus_tag="VF097_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261514.1"
                     /translation="MGPLLPFVVADTPQGPLSYSGIAQEIGGDGWYFIGVGAALVVLG
                     ASIAWSRSATLARVLGAIGLLAAALAAAVAAIDIVDIIGLEEEVPAEAAGLFSFSVGL
                     GLYVAAAGSIVGLIGSATALFVREEAPPPFQPPAATPTGWSPEPEEPPPPPSWPPPSG
                     SPPAAPES"
     gene            complement(575..>4079)
                     /gene="dnaE"
                     /locus_tag="VF097_01570"
     CDS             complement(575..>4079)
                     /gene="dnaE"
                     /locus_tag="VF097_01570"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX6261515.1"
                     /translation="SFVHLHTHTEFSLLDGFARITPGSSAPSMLDRAAEMGMPAIGLT
                     DHGVMFGALDFYREARARGIKPILGVEAYVAPGSRFDRNPGEDEEKYRHLTILARNET
                     GYRNLLRLVSDAHLEGFYHRPRIDKELLARHAEGLIGLSGCLASEVCRHLLADQGKKA
                     EDAAAAYRDIFEEGSFYVELQDHGLADQAKVTPTLIEIARRLGVPLVATNDLHYTHKE
                     DHQAHDVLLCIQQHGKVLTDTNRLRFETEEFYLKSAQEMREVFRELPGACDATLEIAE
                     SVEPIPDLEGVLVDGEVVHRLPRLEPPAGKDQKGYLRELTLEGAQERYGELGPELRAR
                     LEHELTIINEMGFAGYFLVVWDLIRFARERGIRVGPGRGSAAGSVVSYCLRITDLDPL
                     RYNLIFERFLNPERKQMPDIDMDFDERRRDEVIRYVADTYGHDHVAQIITFQTIKGKQ
                     GIRDAARVLGFPAAMGDRLCKMFPAALLGREYPIQQALEISAELKDAYNREDDARTII
                     DTARALEGLRREDSVHAAGVVIGDRPLVNYLPLKLSKDSRDDSKRVVTQFDMHGVEKL
                     GLLKMDFLGLRNLSVIDDTVRMLREQGIELDVDHLPLDDEETYAMLGRGETTGVFQLE
                     GEGMRSLIRQLLPNRFEDLMALVSLYRPGPLNAGMHVEYAERKHGRKPVVYPHPDLEP
                     ILKDTYGVIVYQEQVMEVAVRMAGYSMGEADLLRKAMGKKIREELIPHREKFVAGAVE
                     RGYDRALANEIFDLIMPFADYGFNASHACGYALVAYQTAYLRAHHPVEYMAALLTSVK
                     DDKDKKPFYLNACRLADIRVLPPDVNASAVDFTPVGGEVRFGLSAVRNVGAGAVEQII
                     AARREKGEFTSFADFCRKVDPSVLHKKVLESLILSGAFDSLGHMRRGLLEGYDRVAGP
                     IASQRRAEAAGQFSLFGQGDQASQEIDEAVLEGPEFEKQELLALEKQVLGQYVTDHPL
                     LAVRERLEAQTDVDTSALVSLGDGDVVTVGGIIGSANRRFTKKGEPYAILRLEDLVGG
                     VQVVAFPSVFEKVTDLLAPDRIVLVKGRVDLRGRELQLVAVEIGALDDSGEPPPPAEP
                     ILLEVPAEQCTDGLATALKEVLSGHPGRVAVHLRLRSNGSAKLLRLADGYRVDGSAGL
                     LSELRGLLGAEAVGRAAPAGR"
BASE COUNT          595 a         1474 c         1388 g          622 t
ORIGIN      
        1 agccccgcta gctagctctc gggggctgcc ggggggctcc ccgacggcgg aggccaagac
       61 ggagggggag gaggctcctc gggctcgggc gaccacccgg tcggtgttgc ggctgggggc
      121 tggaagggcg gcggggcctc ctcccgcacg aacagggcgg tcgcgctccc gatcagcccg
      181 acgatcgacc cggcggcggc gacgtagagg ccgaggccca ccgagaagct gaacagcccc
      241 gccgcctccg caggtacctc ctcctccagg ccgatgatgt cgacgatgtc gatcgccgcc
      301 accgcggccg ccagcgccgc ggccaggagg ccgatcgcgc cgagcacgcg cgccaaggtc
      361 gccgaccggc tccatgcgat cgaggcgccg agaacgacca gcgccgcccc caccccgatg
      421 aagtaccagc cgtctccgcc gatctcctgg gcgatgcccg agtagctcag cggcccctgg
      481 ggggtgtcgg cgacgacgaa ggggagcaga ggtcccaccc ccatcacgag accccccaac
      541 acggtcagga tccaggcgag cgagcgcctg gcggtcaacg gcctgcgggg gccgcgcggc
      601 ccacggcctc cgcccccagg aggcccctga gctccgagag gaggccggcg gagccgtcca
      661 cacggtagcc gtccgccagc cgcagcagct tcgcgctccc gttcgaccgg aggcggaggt
      721 ggacggcgac gcgtccaggg tgcccggaga gcacctcctt cagcgccgtc gccaggccgt
      781 ccgtgcactg ctcggccggc acctccagga ggatcggctc ggcgggcggc ggcggctcgc
      841 ccgagtcgtc gagcgccccg atctccacgg ccacgagctg cagctcccgt ccccgcaggt
      901 ccacccgccc cttgacgagc acgatccggt ccggcgccag caggtcggtg accttctcga
      961 acaccgaagg gaaggccacg acctggacgc cgcccacgag atcctccagc cggaggatcg
     1021 cgtagggctc ccccttcttc gtgaaccgcc ggttcgcgga cccgatgatg cctccgaccg
     1081 tgaccacgtc cccgtccccc aggctcacga gcgccgaggt gtccacgtcg gtctgcgcct
     1141 ccagacgctc ccgcaccgcg aggagcgggt ggtcggtgac gtactgcccc agcacctgct
     1201 tctccagcgc cagcagctcc tgcttctcga actcgggccc ctccagcacc gcctcgtcga
     1261 tctcctggga ggcctggtcc ccctgtccga acagcgagaa ctgccccgcg gcctcggccc
     1321 gccgctggga ggcgatcggc cccgccaccc ggtcgtagcc ctcgagcagg ccccgccgca
     1381 tgtgtcccag cgagtcgaac gcgcccgaga ggatcagact ctccagcacc ttcttgtgca
     1441 gcaccgacgg atccaccttc cggcagaagt ccgcgaacga ggtgaactcg cccttctccc
     1501 ggcgcgccgc gatgatctgc tcgaccgcgc ccgcccccac gttccgcacc gccgagagcc
     1561 cgaacctcac ctcgccgccc accggcgtga agtcgacggc tgaggcgttc acgtccggtg
     1621 gcaggacgcg gatgtcggcc aggcggcacg cgttcaggta gaagggcttt ttgtccttgt
     1681 cgtccttgac gctcgtcagc agggcagcca tgtactcgac tggatggtgc gcccggaggt
     1741 acgcggtctg gtaggcgacc agcgcgtacc cgcaggcgtg cgaggcgttg aagccgtagt
     1801 cggcgaacgg catgatcagg tcgaagatct cgttcgcgag cgcccgatca tagccgcgct
     1861 ccacggcgcc ggccacgaac ttctcccggt gcgggatcag ctcctcgcgg atcttcttcc
     1921 ccatggcctt gcggaggagg tcggcctcgc ccatcgagta cccggccatg cgcacggcga
     1981 cctccatgac ctgctcctgg tagacgatga ccccgtaggt gtccttgagg atcggctcca
     2041 ggtcggggtg cgggtacacg accggcttcc ggccgtgctt ccgctccgcg tactccacgt
     2101 gcatcccggc gttgagcggc ccgggacggt agagggagac cagcgccatc aggtcctcga
     2161 agcggttcgg caggagctgg cggatcagcg agcgcatccc ctcgccctcg agctggaaga
     2221 cgccggtcgt ctccccgcgc cccagcatcg cgtacgtctc ctcgtcgtcc agcggcaggt
     2281 ggtcgacgtc gagctcgatc ccctgctccc gcagcatccg cacggtgtcg tcgatcaccg
     2341 agaggttgcg cagcccgagg aagtccatct tgaggaggcc cagcttctcc acgccgtgca
     2401 tgtcgaactg cgtgacgacc cgcttggagt cgtcgcggga gtccttggac agcttcagcg
     2461 ggaggtagtt cacgagcggc cggtctccga tcaccacgcc ggcggcgtgg acggagtcct
     2521 cgcggcggag cccctcgagc gcgcgcgccg tgtcgatgat cgtgcgggcg tcgtcctccc
     2581 ggttgtaggc gtccttcagc tcggccgaga tctccagggc ctgctggatc gggtactcgc
     2641 ggcccaggag cgccgcgggg aacatcttgc acagccgatc ccccatggcc gcggggaacc
     2701 ccagcacccg ggccgcgtcg cggatcccct gcttcccctt gatcgtctgg aacgtgatga
     2761 tctgcgccac gtggtcgtgc ccgtaggtgt ccgcgacgta gcggatcacc tcgtcccgcc
     2821 ggcgctcgtc gaagtccatg tcgatgtcgg gcatctgctt ccgctcgggg ttgaggaagc
     2881 gctcgaagat caggttgtag cggaggggat cgaggtcggt gatgcgcagg cagtacgaga
     2941 ccaccgaccc cgcggccgac ccccggcccg gccccacccg gatcccacgc tctcgcgcga
     3001 agcggatcag gtcccagacg acgaggaagt agcccgcgaa gcccatctcg ttgatgatcg
     3061 tcagctcgtg ttccagcctg gcccggagct cgggcccgag ctcgccgtag cgctcttgcg
     3121 ctccctcgag ggtcagctcg cggaggtagc ccttctggtc cttccccgcg gggggctcca
     3181 gccggggcag gcggtggacc acctcgccgt ccaccaggac gccctcgagg tcggggatcg
     3241 gctccaccga ctcggcgatc tcgagagtgg cgtcgcaggc cccggggagc tcgcggaaga
     3301 cctcgcgcat ctcctgagcc gacttcaggt agaactcctc ggtctcgaac cggagccggt
     3361 tcgtatcggt gaggaccttc ccgtgctgct ggatgcagag cagcacgtcg tgcgcttggt
     3421 ggtcctcctt gtgcgtgtag tggaggtcgt tggtggccac gagcgggacg ccgaggcgac
     3481 gcgcgatctc gatgagcgtc ggggtgacct tggcctggtc ggccagcccg tgatcctgga
     3541 gctcgacgta gaacgagccc tcctcgaaga tgtcgcggta ggcggcggcc gcgtcttcgg
     3601 ccttcttccc ttggtcggcg aggaggtgcc gacacacctc ggaggcgagg cagcccgaga
     3661 gcccgatcag cccttccgcg tggcgggcga gcagttcttt gtcgatcctc ggacggtggt
     3721 agaagccctc gaggtgggcg tcggagacca gccgcaggag gttccggtag cccgtctcgt
     3781 tccgggccag gatcgtgagg tgccggtact tctcctcatc ctcgccgggg ttccgatcga
     3841 agcgggagcc gggcgccacg tacgcctcga ccccgaggat cggcttgatc cctctcgccc
     3901 gggcctcgcg gtagaagtcg agcgccccga acatcacccc gtggtcggtg aggccgatcg
     3961 ccggcatccc catctcggcg gcccggtcga gcatcgaggg cgcgctgctg cccggcgtga
     4021 tgcgcgcgaa cccgtccagg agcgagaact ccgtgtgcgt gtggagatgg acgaagctg
//
LOCUS       DASUTZ010000035         3170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1175218, whole genome shotgun sequence.
ACCESSION   DASUTZ010000035 DASUTZ010000000
VERSION     DASUTZ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175218"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..834)
                     /locus_tag="VF097_01575"
     CDS             complement(124..834)
                     /locus_tag="VF097_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261516.1"
                     /translation="MSREFVEGVLIVVSGFVVFGGSVWLLLSAILGVRMGYLVAATGF
                     FAFLLILSALWTFGAPIPGGQVPAYLGPKGELPHWVPVGAGVQLTSETFPEIERFPGG
                     PWEDPADDPVFADEVEPATGALQEFLAEQAAGELGRQGIEGEVAPETFEVEQLRFATA
                     EDGTELAAARAFATTGGPEVVALAYKNEGNEPLPSWLFLGASVIGLAAHLPFLDRAER
                     RRREFLTGGDQAPWRGPA"
     gene            complement(834..1556)
                     /locus_tag="VF097_01580"
     CDS             complement(834..1556)
                     /locus_tag="VF097_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX6261517.1"
                     /translation="MGIVALSGLAITLIVLGVLVSVAFVAALVRLSLRGRPQAEPDIP
                     AGMRPGPPDEMLERRFVDRFLGWSFLFVIFFAVWLAGLWLMEPQTNADDGVTLTERSV
                     ERGSMWFAISDESNPTGFGCARCHGPAGTGGQQIPFGDGFVQPPALNDVCGGPETGHP
                     LIESIDDVRTTIEQGRPGTAMPSWSIRYEGAMNDQQIDDLLNYLLTIQELPDGAQNVC
                     LEPLEGAEDGAGGNGGGGEGGA"
     gene            complement(1557..2276)
                     /locus_tag="VF097_01585"
     CDS             complement(1557..2276)
                     /locus_tag="VF097_01585"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX6261518.1"
                     /translation="MSRETIAVIIVAVLFGQFLILLVASLVRSRAAARRAEEREAPLE
                     EAKEERATAPVSRRDFFRRSLLASLGLFAAQFGAASLAFLWPTLREGFGSVIDLGLSA
                     DEILNTIQDDRQPYYYGAGRFYLVAYDASQDQQGLYEGIVDGGLMALYQKCVHLGCRV
                     PFCEQSQWFECPCHGSKYNRAGEYRDGPAPRGLDRFAVSIEDGNVTVDTSSIIEGPAR
                     GEVTIQPEPEGPFCINITAEE"
     gene            complement(2273..3106)
                     /locus_tag="VF097_01590"
     CDS             complement(2273..3106)
                     /locus_tag="VF097_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HEX6261519.1"
                     /translation="MAERAEELNKETFERVRDELLQQGLPPRVAEGKARRIAMIEYNK
                     EHGAEPSRPPKEAAPGEAPAQPEGAPAAPAPQQAPQAPQAGGTMAAPPAAALRTAPAP
                     TGKAPVAKPGAPEKHRLLALVPPEGVQRVDREQGDRINVFPHLLLEELLSLIIVTAAL
                     IVFSTFVNAPLRELANQNLTPNPSKAPWYFLGLQELLRYFHPMVAGVTIPTVVFAFLF
                     VLPYVDRNPSNKPGDRKMAIVLWTIFLMFWTILTLIGAFFRGPGFNFVWPWVEGIYFE
                     L"
BASE COUNT          486 a         1110 c         1147 g          427 t
ORIGIN      
        1 gcgcgccatc gagatgagga acacgacgaa gatcaccacg agcatgaggg cgaagaggat
       61 gaacgagagc ccgccctcct cgatcgccag cacgatggtg gtcacgtcca cgtccatctc
      121 ctcctaggca gggccccgcc agggggcctg gtcgccgccg gtcaggaact ccctccgcct
      181 ccgctcggcc cggtccagga acggcaggtg ggccgccagc ccgatcacgg atgcgccgag
      241 gaacagccac gaggggaggg gctcgttgcc ctcgttcttg taagccaggg ccacgacctc
      301 cgggccgccg gtggtggcga aggcccgggc cgccgcgagc tcggtgccat cctcggccgt
      361 ggcgaagcgc agctgctcca cctcgaaggt ctcgggggcc acctcgccct cgataccctg
      421 cctgcccagc tcccccgcgg cctgctcggc caggaactcc tggagggcgc cggtcgcggg
      481 ctcgacctcg tcggcgaaga ccgggtcgtc ggctgggtcc tcccacggcc cgccggggaa
      541 ccgctcgatc tcgggaaagg tctcggaggt gagctgcacc cccgccccca ccgggaccca
      601 gtgcggcagc tcgcccttgg gccccaggta ggcggggacc tgtcccccgg ggatcggggc
      661 gccgaaggtc cagagggcgg agaggatcag gaggaaggcg aagaagccgg tggcggccac
      721 gaggtagccc atccgcacgc cgaggatcgc ggacagcagc agccacaccg accctccgaa
      781 gaccacgaag cccgacacga cgatgaggac gccctcgacg aactcgcggc tcatcaggcg
      841 cccccctcgc ccccgccgcc gttcccgccg gcgccgtcct cggccccctc cagcggctcc
      901 aggcacacgt tctgcgcgcc gtccgggagc tcctggatgg tgagcaggta gttcaggagg
      961 tcgtcgatct gctggtcgtt catggcgccc tcgtagcgga tgctccacga gggcatcgcc
     1021 gtcccaggac ggccctgctc gatcgtcgtc ctgacgtcgt cgatggactc gatcagcggg
     1081 tggcccgtct cgggcccgcc gcagacgtca ttgagggccg gcggctgcac gaacccgtct
     1141 ccgaacggga tctgctggcc ccccgtgccg gccggaccgt ggcaccgcgc gcagccgaag
     1201 ccggtggggt tggactcatc ggagatggcg aaccacatcg agccccgctc caccgagcgc
     1261 tcggtgagcg tgacgccgtc gtcggcgttc gtctggggct ccatcagcca gagcccggcc
     1321 agccacacgg cgaagaagat cacgaagagg aacgaccagc ccaggaagcg gtccacgaag
     1381 cgccgctcca gcatctcgtc gggtgggccg gggcgcatcc ccgcagggat gtcgggctcg
     1441 gcctgggggc gcccgcggag actcagccgc acgagggcgg ccacgaacgc caccgatacg
     1501 agcacgccca ggacgatgag ggtgatcgcc aggccggaga gcgcgacgat gcccacctac
     1561 tcctccgccg tgatgttgat gcagaagggt ccctcgggct cgggctggat cgtcacctct
     1621 ccccgggccg gcccctcgat gatcgagctc gtgtccacgg tcacgttgcc gtcctcgatc
     1681 gagacggcga agcggtcgag gccccggggg gcggggccgt cgcggtactc gccggcccgg
     1741 ttgtacttgg agccgtggca ggggcactcg aaccactggc tctgctcgca gaacggcacc
     1801 cggcacccca ggtgtacgca cttctggtag agggccatca gcccgccgtc cacgatcccc
     1861 tcgtacaggc cctgctgatc ctgggaggcg tcgtaggcca cgaggtagaa gcgccccgcg
     1921 ccgtagtagt agggctggcg gtcgtcctgg atcgtgttga ggatctcgtc ggcgctgagg
     1981 ccgaggtcga tcaccgatcc gaacccctcg cggagggtgg gccagaggaa ggccaggctg
     2041 gcggcgccga actgcgccgc gaacaggccc agcgaggcca gcagcgagcg ccggaagaag
     2101 tcccggcgcg acaccggggc ggtcgcgcgc tcctccttgg cctcctcgag gggcgcctcg
     2161 cgctcctcgg cccggcgggc ggcggcccgc gaccggacca gggaggccac gagcaggatc
     2221 aggaactgcc cgaacaggac cgcgacgatg atgacggcga tggtctctct gctcatagct
     2281 cgaagtagat gccctcgacc cagggccaga cgaagttgaa ccccggcccc cggaagaacg
     2341 cgccgatcag ggtcaggatc gtccagaaca tgaggaagat cgtccacagc acgatggcca
     2401 tcttgcggtc gccgggcttg ttgctggggt tgcggtccac gtaggggagg acgaacagga
     2461 acgcgaacac gaccgtcggg atcgtcacgc cggccaccat ggggtggaag tagcgcagca
     2521 gctcctgcag ccccaggaag taccaggggg ccttggacgg gttcggcgtc aggttctggt
     2581 tggccagctc ccgcagcggc gcgttcacga acgtggagaa gacgatcagc gccgcggtca
     2641 cgatgatcag cgacagcagc tcctcgagga gcaggtgggg gaagacgttg atccggtcgc
     2701 cctgctcgcg atccacgcgc tggacgcctt cgggcgggac gagcgccagg aggcggtgct
     2761 tctcgggcgc gcccggcttg gcgaccggcg ccttgccggt gggggcgggc gcggtgcgca
     2821 gggccgcggc gggcggggcg gccatcgtcc caccggcctg gggggcctgg ggcgcctgct
     2881 ggggcgccgg ggccgcgggg gcgccctcgg gctgggccgg cgcctcgccg ggggcggcct
     2941 ccttcggagg ccgggagggc tcggcgccgt gctccttgtt gtactcgatc atggcgatgc
     3001 gccgggcctt cccctcggcc acgcggggag gcaggccctg ctggagcagc tcgtcgcgga
     3061 cccgctcgaa ggtctccttg ttgagctctt cagctcgctc ggccatcccg cgctcctaga
     3121 ggggtccgga gatcccgccg tccttgcgga tccgccagaa gtggatcgcc
//
LOCUS       DASUTZ010000036        15059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1823300, whole genome shotgun sequence.
ACCESSION   DASUTZ010000036 DASUTZ010000000
VERSION     DASUTZ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 15059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15059
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1823300"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..2262
                     /locus_tag="VF097_01595"
     CDS             82..2262
                     /locus_tag="VF097_01595"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008406475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX6261520.1"
                     /translation="MSSHPDRAEGFDGLLRKVRAINQKADIRLIERAYEVAERAHHGQ
                     LRKSGEAFIEHPLGVTHVLADLGMDTTTLVAGILHDVVEDTSVTLEEVEREFGADVAA
                     LVDGVTKLDKIPLRTREQHQAENVRKMIVAMARDIRVLIIKLADRLHNMRTLKALRPE
                     KQRQKATETLEIYAPLAHRLGIYSIRWELEDLAFATLHPRRFEEISNLVGKAEDERES
                     QLEEARASIGKHLKKLKIKAEVTGRPKHLYGVYEKMVLRGKEFSEIYDLLGIRVLCDT
                     VKDCYAALGAVHSMWKPVPGRFKDYVAMPKFNMYQSLHTVVIGPGGKPLEVQIRTEQM
                     HKTAEFGIAAHWQYKEGKKGKRDETDLAWLRQIMEWQQETQDPKDFMESLRIDLYEDQ
                     IFVFTPKGDVIDLPAGATPIDFAYNIHTEVGHRCMGAKVNGKLVPLDYALRTGDTVEV
                     LTSKAGDAGPSRDWLRVVKSPRARNKIRQWFSRERREDAMETGRDLLQRELRRQGLPL
                     KRLTTEQVLADLAEGLNYADLDSLLVAIGEGHVSPKSIVVRLERIVTQEDEEEADVPP
                     ARPVAIGKQDAGGAVVVQGSSDVWVKLARCCTPVPGDEIMGFVTRGQGVSVHRADCPN
                     AKALESQRDRFIEVSWRTGKPTSFIVSIMVEALDRKKLLGDIATALSEQQVNIISASS
                     GVGKDRVATFRFTFELADIAHLESVLRAIKKVDAVFDAYREVPG"
     gene            2259..2696
                     /gene="dtd"
                     /locus_tag="VF097_01600"
     CDS             2259..2696
                     /gene="dtd"
                     /locus_tag="VF097_01600"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014464963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX6261521.1"
                     /translation="MRIVLQRVARAEVRVGGETVASCGGGLLLLAGVGAEDDGSQTPG
                     LAAKIAGLRVFEDEKGRMNRSLDEVSGQVLVAPNFTLYGDTRKGRRPSWVGAAPPEVA
                     EGHVEALCRALEGHGLTVHRGVFGAHMEIELVNDGPVTLVLEA"
     gene            2728..3351
                     /locus_tag="VF097_01605"
     CDS             2728..3351
                     /locus_tag="VF097_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6261522.1"
                     /translation="MFLDVRFDNPFQTNCWAVSADGSEETVVVDPGFQPEQVRDMVER
                     AGRRIVAVLATHGHADHIASVPAVCGPDIPLYIHEADELALTDQGAWGAGMPVPDRYR
                     PEEVRTVKDGDVLEFAGFRIRVRHTPGHTPGSVCYVTDGFLFSGDLVFRDTIGRHDFP
                     NSSSRDMFESLRWFLTLRDDLDVYPGHGPQTTVGRERARNEWLRALV"
     gene            3348..4607
                     /gene="hisS"
                     /locus_tag="VF097_01610"
     CDS             3348..4607
                     /gene="hisS"
                     /locus_tag="VF097_01610"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019909088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX6261523.1"
                     /translation="MSERFAPPRGTQDLLPPTSDRLEVLASEAARLARAFGYRRVETP
                     TFEHTELFARGVGETTDIVTKEMYTFQDKGGRSLTLRPEGTTGVVRAYLGNRQGLPTP
                     FKGYYVAPMFRHERPQAGRMRQHTQFGIEVIGTPAPDADAEVIAVGHRFLSRWGLSFE
                     LRVNSIGDPVCRPAYREALLAFLRERRDRLSADCRERMEENPLRVLDCKVPSCREATA
                     EAPSILDFLDQACREHFEAVLERLDEQGIEHALDPRLVRGLDYYTRTAFEFVSPDLGP
                     TQSTLCGGGRYDGLAEAIGGPATPGIGFGLGLERVLLAMERAGLVPPETPGTTVFVVA
                     LGDEAGREGRALARALRDEGIPAEHALEPRPLGAQLKMADRAGAAYAAILGDDELAAG
                     EVTMRDMETGEQERVKTEGVASWLTSR"
     gene            4589..6358
                     /gene="aspS"
                     /locus_tag="VF097_01615"
     CDS             4589..6358
                     /gene="aspS"
                     /locus_tag="VF097_01615"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX6261524.1"
                     /translation="MADLPMRTHMCGGLGLEHAGEQVALCGWISTTRDHGGVIFVDLR
                     DREGEIQVVVHPQEAPRAHEAAQRLKTESVVRAVGVVRPRPEGTVNPDLPTGQVEVAA
                     EAIEVLSEAETPPFPVEDRITADEALRLRHRYLDLRRPQMTRVLRVRHAAAHAIRGYM
                     ERNGFVEVETPMLTRSTPEGARDFLVPSRLQPGSFYALPQSPQLFKQILMVSGLDRYY
                     QIVRCFRDEDLRADRQPEFTQLDVELSFTDEAQVQELVEGLLAEVWEEVLGVTVATRF
                     PRLTYDESMRRFGTDKPDLRYGMELADLTEAFRESGFNAFRTVVEEGGLVKGLAAPGA
                     AGWSRKDLDQLVVEAQARGAGGLVWIALAEGEVKSPVAKLLSGEELAAARSATGAGEG
                     DLILLVADTEARAGTVLDGMRRLMADRLRLIPEGEWVFLWITEPPLFEWSEEEERWVS
                     VHHPFTSPATDDLEPASAKARAYDVVLNGWELGGGSIRIHRPEIQRKVFDALGMSREE
                     AEERFGFLLEAFRYGVPPHGGIAFGLDRMVMLLAGMENIRDVIAFPKTQSGADLLTGA
                     PAPVSEAQLRELGLRLRGRPEAP"
     gene            6654..7829
                     /locus_tag="VF097_01620"
     CDS             6654..7829
                     /locus_tag="VF097_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017625759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX6261525.1"
                     /translation="MAPEPLASRIRPRSLDEVVGQRHLLGEGKALAALVGADHLPSIV
                     LWGPAGTGKTTLAHLLAKAVGAELVQLSAVSSGVADARKVLDSARGGMLQTVLFVDEV
                     HRWSKAQQDVLLPGVEAGDVTFIGATTENPYFSLVSPLLSRSLLLRLEPLTDEEVRTI
                     LERALADERGLPGTKVEDEALAYLAETAGGDARLALAGLEAAARVAAGRPVTREIAED
                     VVQRRGLVYDRDEHYDVTSAFIKSMRGSDADASLFWLARMLEAGEDPRFIARRMVIFA
                     SEDVGLADPLALQIAVAAAQAVEFVGLPEVRLNLAHAAAYLARAPKSNAVIRAIGKAD
                     EAQRRAGRVPTHLRDAHYAGARDLGHGEGYAYPPSDPEGAESQRYLPEGVEGGFLDE"
     gene            7887..8240
                     /locus_tag="VF097_01625"
     CDS             7887..8240
                     /locus_tag="VF097_01625"
                     /inference="COORDINATES: protein motif:HMM:NF017876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEX6261526.1"
                     /translation="MSAGDWALIVLAVFWAVLVVFLALVLVQLVRVLESTKILIDGIR
                     SETVPLLGEVTTSVKGVNRELDRVDDIVVSAASITKSAERLTSVVERAISSPLVKAIA
                     LGAGASRAFRRLRGS"
     gene            8240..8470
                     /locus_tag="VF097_01630"
     CDS             8240..8470
                     /locus_tag="VF097_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261527.1"
                     /translation="MKRIFWLAMGLGAGVTAAVMLSRWMRRQQERLSPAAIGAQVGDT
                     ARDLGRLLRTALDEGREEMRRVEAEIRSSIQE"
     gene            8490..11108
                     /gene="alaS"
                     /locus_tag="VF097_01635"
     CDS             8490..11108
                     /gene="alaS"
                     /locus_tag="VF097_01635"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018852905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX6261528.1"
                     /translation="MEGAEIRRRFLRHFEERDHTIVPSSSLIPNDPTLLLTTAGMVQF
                     KPYFLGEQEPPYPRAASAQKSFRTTDIELVGHTDRHLTFFEMLGNFSFGDYFKEEAVR
                     LAWDLVTEGFEIDPDRLWATVYETDDDAEEIWKAYLPADRVVRRGRADNFWDMGVAGP
                     CGPCSELYVDRGPEHGTEGGPEADEDRYLEIWNLVFMQNECDADIEVAGDLPRKNIDT
                     GMGLERMALVLQEAGSAFETDLLRPLLEEAERLADRRYGVDETEDVSLRILAEHGRAT
                     TFLIADGVVPSNEGRGYVLRRMLRRVVSHARRLGVEGVVLRPLVERTVEMLGDAYPEL
                     VERRAFVLQVVSSEEEQFARTLAQGMELFEREAERAREAGERAFPGEAAFLYHDTYGF
                     PIELTQELAREHGLEVDEAAFQRLMEEQRTRARRAAKGGADDRALLEAARAAGRTEFV
                     GYAEAAAEGKVLALVRKGERVEAGGEGEEIELLLDRTPFYAEGGGQVGDAGAIRTETG
                     VVSVADTVPGPGEVIVHRGTVNEGEIRAGQEAHLQIDLPRREATARSHTGTHVLHWTL
                     RHLLGEHARQAGSLVAPGRLRFDFTHFEGLSPDLLEELEATANARLGEDASVRAYETT
                     FEYARSQGALALFGEQYGDLVRVVEIGDYSVELCGGTHVSHTGEVSLMVFGSEGSIGS
                     GLRRVEALVGPDALAHVHAERRLLEELTEILGAQGPQQAVERARRAVARIKELEGQLG
                     ELRQQERGELVDALLRETADVGGVSLVVARLDEDPGTLREMALKLRDRLQGGPGAAVL
                     ASADGRKATLVASATPELIDRGVTAPGLLEDAARAVGGGAGGKHHLAFAGGGSPDALD
                     EALRVLPDRLLALLGG"
     gene            11105..11530
                     /gene="ruvX"
                     /locus_tag="VF097_01640"
     CDS             11105..11530
                     /gene="ruvX"
                     /locus_tag="VF097_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX6261529.1"
                     /translation="MTAPGRVLALDVGKARVGVAVSDPDRTVALPAGTIRVVGGIQDL
                     RAVARLVERYEAVVVVVGHPLSLSGERGPAARHAEELADGLRAILDVPVHLQDERLST
                     IEAERGLAAAGARGPDRRRSVDQAAASIILRAFLDRTAG"
     gene            11568..12620
                     /gene="mltG"
                     /locus_tag="VF097_01645"
     CDS             11568..12620
                     /gene="mltG"
                     /locus_tag="VF097_01645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX6261530.1"
                     /translation="MLDQVGGAHRARGRALRVLLVLFLVLALLLAGAFGFYVWATGGS
                     GPSRPVTLEIPQGATVSEMGAALEEAGVIRSAFMFGITVRLQGIGGELKAGTYDNMRT
                     NMRLAEALDLLQAGPPPEKLPLSVTVPEGLRIEQVAEVLAEGLGIRPRAFLREARSGE
                     WAIEPWLPEGPETVEGFLFPKTYDFPEGVRARAVLRRMLAQFEEEVSGLDWSRAEALG
                     LEPYEVVVMASLIEREARVPKDRRKISAVIHNRLERDMPLEIDATVQYALPEHKERLT
                     FDDLEYESPYNTYLFVGLPPGPIASPGVASIEAAMNPANGDWLYYVLIDPETGEHAFA
                     ETYEEFLEYREQAGLG"
     gene            12617..13423
                     /gene="aroE"
                     /locus_tag="VF097_01650"
     CDS             12617..13423
                     /gene="aroE"
                     /locus_tag="VF097_01650"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="AroE; catalyzes the conversion of shikimate to
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX6261531.1"
                     /translation="MSTALYAVMGWPVVHSASPAIHTAAFDTLGIPAVYLRLPVPPGR
                     AAAAIASLWALGARGASVTAPHKVAIAGLCQDLARSARRTGTVNTLLIEEGSVTGHDT
                     DAEGLRRFLLEDARLDLAGLTALVLGGGGAARACAVALSGLAGRLVVAARGAAAGAEV
                     ADLFEGPSTVIPFVEAGEAQADLLLNATPLGWRGEDVAVDPRPGQVVVDLVYRETPLL
                     RRAWAVGAVAHDGRGMLLRQAALSFRLWTGEDPPLEAMRAALDGVLTSAD"
     gene            13480..14325
                     /locus_tag="VF097_01655"
     CDS             13480..14325
                     /locus_tag="VF097_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017610425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261532.1"
                     /translation="MPGETIDFEALAARLSEIDGVRACLLMSRDGLTLGVHPADREES
                     ARAVWEGMHSIGDPERGFLTVGDELWIVARRGPYAGIVVAGPSATPGLLLDRLEFSLR
                     AAEELRLREGSAATSPRAEGSRKPRSPLHPETGLAPLTHEPDGSSDAVSAAIQIASMR
                     EGPITLSPRDGNGHRNGSKPPELKLRPLETEAPLEPEPDPEPDAEPAPEPDAEPAPER
                     GLDIDLDALPEPEVEIEIRPVPDEEEPTLGSGEHFPLPAGDEREVDPVALAREFKNLF
                     DRPDE"
     gene            14370..>15059
                     /locus_tag="VF097_01660"
     CDS             14370..>15059
                     /locus_tag="VF097_01660"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX6261533.1"
                     /translation="MRKTKDLGEVLVDEGLITEDDLGKAHEEQERSGRSLGRVLLDLN
                     LVDETKLVEALAHQIGLQFVDLSEHGVDASAVALVPEQVARRHRAIPIAFEDSTLVVA
                     MSDPSNVIALDDIRTITGFEVRAVVATAADVDEAINKYAKLDQSVEDIAHQAAGAADE
                     DLDLDAMEAAVEEAPIVKLVNLLITQAVADRASDIHVEPAEKDVRIRYRIDGVLHEVM
                     RSPKNIQNGLVS"
BASE COUNT         2196 a         5219 c         5525 g         2119 t
ORIGIN      
        1 ggaggtcagg agaggggtgc actcgtggag gacgttcgcg caccggagcg acggagcggc
       61 ctcctggcgc gccttccctt cgtgtcgagc catcccgatc gagccgaggg gttcgacggg
      121 ctcctcagga aggtgcgggc gatcaaccag aaggccgaca tccggctgat cgagcgcgcc
      181 tacgaggtag ccgagcgcgc ccaccatggc cagctccgaa agtccggcga ggccttcatc
      241 gagcacccgc tcggcgtgac tcacgtcctg gccgacctgg ggatggacac gaccacgctg
      301 gtggcgggga tcctccacga cgtcgtcgag gacacgagcg tcaccctcga ggaggtggag
      361 cgggagttcg gggccgacgt ggccgcgctg gtggacgggg tcacgaagct ggacaagatc
      421 cccctccgaa cccgcgagca gcaccaggcg gagaacgtcc ggaagatgat cgtggccatg
      481 gcccgcgaca tccgggtgct gatcatcaag ctggccgacc gcctccacaa catgcgcacg
      541 ctgaaggccc tgcgccccga gaagcagcga cagaaggcca ccgagaccct ggagatctac
      601 gcgcccctcg cgcaccggct cggcatctac tcgatcaggt gggagctcga ggacctggcc
      661 ttcgccaccc tccacccccg tcgtttcgag gagatcagca acctcgtggg gaaggccgag
      721 gacgagcgcg aatcccagct cgaggaggcc cgggcctcga tcgggaagca cctcaagaag
      781 ctgaagatca aggccgaggt cacgggccgc cccaagcacc tctacggggt ctacgagaag
      841 atggtcttgc ggggcaagga gttcagcgag atctacgatc tcctcggcat ccgcgtcctc
      901 tgcgacacgg tcaaggactg ctacgcggcg ttgggtgccg tccactcgat gtggaagccc
      961 gtccccggtc gcttcaagga ctacgtggcg atgcccaagt tcaacatgta ccagtccctg
     1021 catacggtcg tgatcgggcc cggaggcaag cccctcgagg tgcagatccg caccgagcag
     1081 atgcacaaga ccgccgaatt cgggatcgcc gcgcactggc agtacaagga ggggaagaag
     1141 gggaagcgcg acgagaccga tctggcgtgg ctccgccaga tcatggagtg gcagcaggag
     1201 acgcaggacc cgaaggactt catggagtcc ctgcggatcg atctctacga ggaccagatc
     1261 ttcgtcttca cgccgaaggg cgacgtgatc gacctgccgg ccggcgccac tccgatcgac
     1321 ttcgcctaca acatccacac ggaggtcggc caccgctgca tgggggcgaa ggtgaacggc
     1381 aagctcgtgc cgctcgacta cgcgctgcgg acgggcgaca cggtggaggt gctcacctcg
     1441 aaggcgggcg acgcgggtcc ctcgcgcgac tggctcaggg tggtgaagtc gccccgggcc
     1501 cgcaacaaga tccggcagtg gttctcccgg gagcgtcgcg aggacgccat ggagacgggc
     1561 agggacttgc tccagcgcga gctgcgccgc cagggcctcc ctttgaagcg gctcaccacc
     1621 gagcaggtgc tggccgacct ggccgagggc ctcaactacg cggacctcga ctcgctgctg
     1681 gtggcgatcg gcgaggggca cgtctctccg aagtcgatcg tggtgcggct ggagcggatc
     1741 gtcacccagg aggacgagga ggaggccgac gttccgccgg cccgcccggt ggcgatcggg
     1801 aagcaggacg cgggcggggc ggtggtcgtc caggggtcct cggacgtctg ggtgaagctg
     1861 gctcggtgct gcacgccggt gccgggcgac gagatcatgg ggttcgtgac gcgcggccag
     1921 ggcgtatccg tccaccgagc cgactgtccg aacgccaagg ccctggagag ccagcgggac
     1981 cggttcatcg aggtctcctg gcgcaccggg aagccaacct cattcatcgt ctcgatcatg
     2041 gtcgaggccc tggaccggaa gaagctgctc ggagacatcg ccacggcgct ctccgagcag
     2101 caggtcaata tcatctcggc ctcctccggg gtggggaagg accgcgtggc cacgttccgg
     2161 ttcacgttcg agctcgccga tatcgcccac ctggagagcg tcctccgcgc gatcaagaag
     2221 gtggacgccg tgttcgacgc ctaccgcgag gtccccggct gaggatcgtc ctccagcggg
     2281 tggcccgggc cgaggtccgc gtggggggcg agaccgtggc ctcctgcggc ggcgggctcc
     2341 tgctgctggc gggcgtggga gccgaggacg acggctccca gacccccggc ctcgccgcga
     2401 agatcgccgg cctgcgggtg ttcgaggacg agaagggcag gatgaaccga tcgctggacg
     2461 aggtctcggg ccaggtcctc gtcgccccta acttcactct gtacggcgac acgcggaagg
     2521 gccggcggcc gtcctgggtc ggggccgcgc cgcccgaggt ggccgagggg cacgtggagg
     2581 ccctctgccg cgccctcgag ggccatggcc tcaccgtgca tcgtggcgtg ttcggcgccc
     2641 acatggagat cgagctggtc aacgacgggc ctgtcacgct cgtcctcgag gcctgagcgt
     2701 cctcgcgtat ccactacgat gagccggatg ttcctcgacg tgcgcttcga caaccccttc
     2761 cagacgaact gctgggctgt ctcggccgac ggctccgaag agacagtggt tgtggacccg
     2821 ggcttccagc ccgagcaggt ccgcgacatg gtggagcgcg ccggacgccg catcgtggcg
     2881 gtcctggcca cgcacgggca tgccgaccac atcgcctcgg tgcccgccgt ctgcgggccc
     2941 gacatccccc tctacatcca cgaggccgac gagctggcgc tgaccgacca gggcgcctgg
     3001 ggtgccggga tgccggtccc ggaccggtac cggcccgagg aggtccgcac ggtgaaggat
     3061 ggcgacgtcc tggagttcgc gggcttccgg atccgagtcc gtcacactcc cgggcacacg
     3121 cccggcagcg tctgctacgt gaccgacggg ttcctcttct cgggagacct cgtgttccgc
     3181 gacacgatcg ggcggcacga cttccccaac tcgagctccc gggacatgtt cgagagcctg
     3241 cgctggttcc tcacgctccg ggacgacctg gacgtctacc cggggcacgg gccccagacc
     3301 accgtggggc gggagcgggc ccggaacgag tggctcaggg cgctggtgtg agcgagcggt
     3361 tcgcccctcc gcgcggcacc caggacctgc tgccgccgac ctccgaccgc ctcgaggtgt
     3421 tggcctcgga ggccgcgcgc ctggcgcggg cgttcgggta ccggcgggtc gagacgccca
     3481 ccttcgagca caccgagctg ttcgcccgag gtgtgggcga gaccaccgac atcgtcacca
     3541 aggagatgta cacgttccag gacaagggcg gtcgctcgct gaccctccgg cccgagggca
     3601 ccacgggcgt cgtccgggcg tacctgggga accggcaggg gctgcccacc cccttcaagg
     3661 gctactacgt cgcgccgatg ttccgccacg agcgtcccca ggcgggccgc atgcgccagc
     3721 acacgcagtt cgggatcgag gtcatcggca cgccggcccc ggacgcggac gccgaggtga
     3781 tcgcggtcgg gcaccgcttc ctctcccggt gggggctctc gttcgagctg cgggtgaact
     3841 cgatcgggga tccggtttgc cgtcccgcct accgcgaggc cctgctagcc ttcctccggg
     3901 agcggcgcga ccggctctcc gccgactgcc gcgagcggat ggaggagaac cccctccggg
     3961 tgctggactg caaggtgccc tcgtgccgag aggcgaccgc cgaggcaccg agcatcctcg
     4021 acttcctcga ccaggcctgc cgggagcact tcgaggccgt cctcgagcgg ctcgacgagc
     4081 aggggatcga gcacgccctt gaccccaggc tcgtgcgggg cctcgactac tacacacgga
     4141 ccgcgttcga gttcgtgagc ccggacctcg ggccgaccca gtccacgctg tgcgggggag
     4201 ggcggtacga cgggctggcc gaggcgatcg gggggccggc aacgccgggg atcggcttcg
     4261 gcctgggact ggagcgggtc ctcctggcga tggagcgagc gggcctggtc cccccggaga
     4321 cgcccggcac cacggtgttc gtcgtcgcgc tcggcgacga ggccgggcgg gagggacgcg
     4381 cgctggcccg ggccctccgc gacgagggga tcccggccga gcacgccctc gaaccccgcc
     4441 cgctcggggc ccagctgaag atggccgacc gggccggggc ggcctacgcg gcgatcctgg
     4501 gcgacgacga gctggccgcc ggcgaggtca cgatgcgcga catggagacc ggggagcagg
     4561 agcgagtcaa gacggagggt gtggcctcgt ggctgacctc ccgatgagga cgcacatgtg
     4621 cggcggtctc ggtctggagc acgccggcga gcaggtggca ctctgcggct ggatctccac
     4681 gacccgggac cacggcggcg tgatcttcgt ggacctccga gatcgggagg gagagatcca
     4741 ggtcgtggtc catccccagg aggcccctcg tgcccacgag gcggcccagc gcctcaagac
     4801 cgagagcgtg gtgcgggccg tgggcgtcgt gcggccccgc ccggagggca ccgtcaaccc
     4861 ggacctgccc accggccagg tggaggtggc cgccgaggcg atcgaggtgc tgagcgaggc
     4921 cgagaccccg ccgttcccgg tcgaggaccg gatcaccgcc gacgaggccc tccgcctgcg
     4981 tcaccgctac ctcgacctcc ggcgccccca gatgacccgc gtcctccgag tgcgccacgc
     5041 ggcggcccac gcgatccggg gctacatgga gcggaacggg ttcgttgagg tcgagacacc
     5101 gatgctgacc aggtcgacgc ccgagggggc ccgcgacttc ctcgtgccct ccaggctcca
     5161 gccgggctcg ttctacgccc tcccacagtc gccccagctc ttcaagcaga tcctcatggt
     5221 ctcggggctg gaccgctact atcagatcgt ccgctgcttc cgggacgagg acctccgggc
     5281 cgaccgccag cccgagttca cccagctcga cgtggagctt tcgttcaccg acgaggccca
     5341 ggtccaggag ctcgtggagg ggctgctggc ggaggtgtgg gaggaggtcc tgggggtgac
     5401 ggtcgccacg cggttcccca ggctcaccta cgacgagtcg atgcgtcggt tcgggaccga
     5461 caagcccgac ctccgctacg ggatggagct ggccgacctc accgaggcgt ttcgggagag
     5521 cgggttcaac gccttccgca cggtggtgga ggaggggggc ctcgtcaagg ggctcgcggc
     5581 ccccggcgcc gcgggctggt cgcggaagga cctggatcag cttgtggtcg aggcccaggc
     5641 ccgcggcgcc gggggcctgg tctggatcgc tctcgccgag ggcgaggtca agtccccggt
     5701 ggcgaagctc ctctccgggg aggagctggc cgccgcgcgc tcggcgaccg gggcagggga
     5761 gggcgacctc atcctcctcg tggccgacac cgaggcccgt gcaggcaccg tgctcgacgg
     5821 gatgcgccgg ctcatggcgg accgcctccg gttgatcccc gagggcgagt gggtgttcct
     5881 ctggatcacg gagccgcccc tgttcgagtg gagcgaggag gaagagcggt gggtgtccgt
     5941 gcaccacccc ttcacgtcgc ccgcgaccga cgacctggag cccgcctccg cgaaggcccg
     6001 cgcctacgac gtcgtgctca acggttggga gctcgggggc ggcagcatcc ggatccaccg
     6061 ccccgagatc cagcggaagg tgttcgacgc cctggggatg tcccgggagg aggcagagga
     6121 gcggttcggc ttcctcctgg aggccttccg ctacggcgtc ccgccccacg gggggatcgc
     6181 cttcggcctg gaccgcatgg tcatgctgct cgcggggatg gagaacatcc gcgacgtgat
     6241 cgccttcccc aagacccagt ccggggccga cctgctcaca ggggcgccgg ccccagtgtc
     6301 cgaggcccag ctgcgggagc tcggcctccg tctccggggg cgacccgagg ctccctgacc
     6361 ggcggcacgc cggcggcctg ggcccgtgca ggtgctcacg atgagcccgg cgctcgcgcc
     6421 gcgggcggac ccatgatctc gccgtctctt cgctccacgg gtgtaaggta ggggtgcgga
     6481 tgagatacgg ctcctgatct cgttgagcca acaccatgaa gaggggagcc tggctccgcc
     6541 aggggtgctg agccccgaga ggggttctct gaaccgggcg ggagggcacc cacctgcgag
     6601 agcaggccca tcgagagcac gagtcgcctc atccgcatcc ctatgatggg gccgtggccc
     6661 ccgagcccct ggcatcccgg atccgccccc gatccctgga cgaggtcgtc gggcagcggc
     6721 acctcctcgg cgaggggaag gcgctggccg cgctggtggg ggccgaccac ctcccatcga
     6781 tcgtgctctg ggggccggcc gggacgggga agaccaccct ggcccacctc ctggcgaagg
     6841 cggtgggggc cgagctggtc cagctgtcgg cggtgtcgtc gggcgtggcc gatgcgcgca
     6901 aggtgctgga ctccgcgcgg ggcgggatgc tccagaccgt gctcttcgtg gacgaggtgc
     6961 accgctggtc gaaggcccag caggatgttc tgctgccggg cgtcgaggcc ggggacgtca
     7021 ccttcatcgg ggccaccacc gagaacccct acttctcgct cgtctccccc ctgctgagca
     7081 ggtcgctgct gctccggctc gagcccctga ccgacgaaga ggtgcgcacg atcctggagc
     7141 gggcgctcgc cgacgagcga gggctccccg ggacgaaggt cgaggacgag gcgctggcct
     7201 atctggcgga gacggccggc ggcgacgcgc gcctggccct ggcgggcctc gaggcagcgg
     7261 cgcgcgtcgc cgccggccgt cccgtcacgc gggagatcgc cgaggacgtc gtccagcgcc
     7321 gaggcctcgt ctacgaccgc gacgagcact acgacgtgac ctcggccttc atcaagtcga
     7381 tgcggggctc ggacgcagac gcctcgctct tctggctggc ccggatgctg gaggcggggg
     7441 aggaccctcg attcatcgcc cgccgcatgg tcatcttcgc ctccgaggac gtgggcctgg
     7501 cggaccccct cgccctgcag atcgcggtgg ccgccgcgca ggcggtcgag ttcgtcggcc
     7561 tcccggaggt ccgcctcaac ctggctcacg ccgccgccta tctggccagg gcccccaagt
     7621 ccaacgccgt gatccgcgcg atcggaaagg cggacgaggc tcagcgaaga gccggccggg
     7681 tgcccaccca tctccgggac gcgcactacg ccggggccag ggacctgggc cacggcgagg
     7741 gctacgccta ccctccgagc gacccagagg gagccgagag ccagcgctac ctcccggaag
     7801 gcgtcgaggg cggcttcctc gacgagtagc gtttcggtgg ctccgccctc cacgggtatg
     7861 atgtggcgac ccgacgaagg agcgacatgt ctgcgggtga ctgggccctg atcgtcttgg
     7921 ccgtcttctg ggcggtcctc gtcgtcttcc tcgccctcgt gctcgtccag ctcgtgcgcg
     7981 tgctggagag cacgaagatc ctgatcgacg ggatccgcag cgagacggtc cccctgctcg
     8041 gtgaggtcac cacctcggtc aagggcgtga accgcgagct tgaccgcgtg gacgacatcg
     8101 tcgtctccgc cgcgagcatc acgaagtcgg cggagcgtct cacctcggtc gtcgagcggg
     8161 cgatctcgag cccgctggtg aaggcgatcg cgctcggcgc gggtgcctca agggccttcc
     8221 ggcgcctccg agggtcgtga tgaagcggat cttctggctg gccatgggcc tgggggcggg
     8281 ggtgacggcc gcggtgatgc tctcccgttg gatgaggcgg cagcaggagc gtctctcgcc
     8341 ggccgccatc ggcgcgcagg tcggcgatac ggcgcgcgac ctggggcgtc tgcttcggac
     8401 cgccctcgac gaggggcgcg aggagatgcg ccgggtcgag gccgagatcc gctcctcgat
     8461 ccaggagtag cccccgctag acttgccgca tggaaggcgc cgagatccgt cgccgcttcc
     8521 tgcggcactt cgaggagcgc gaccacacga tcgtgccctc ctcgtcgctg atcccgaacg
     8581 accccacgct cctgctgacc accgcgggca tggtccagtt caagccgtac ttcctcgggg
     8641 agcaggaacc cccgtaccct cgcgcggcca gcgcgcagaa gtcgttccgc acgaccgaca
     8701 tcgagctcgt ggggcacacg gatcgccacc tcacgttctt cgagatgctc ggcaacttct
     8761 cgttcggcga ctacttcaag gaggaggccg tccgcctggc ctgggacctc gtcaccgagg
     8821 ggttcgaaat cgaccccgac cgcctctggg ccaccgtcta cgagaccgac gacgacgccg
     8881 aggagatctg gaaggcctac ctacccgccg accgggtcgt gcggcggggg agggccgaca
     8941 acttctggga catgggcgtg gccggtccct gcgggccctg ctcggagctc tacgtggatc
     9001 gggggccgga gcacggcacg gagggcgggc cggaggccga cgaggaccgg tatctcgaga
     9061 tctggaacct cgtcttcatg cagaacgagt gcgacgcgga catcgaggtc gccggcgacc
     9121 tgccacggaa gaacatcgac accgggatgg ggctggagcg gatggccctg gtcctgcagg
     9181 aggccgggtc cgcgttcgag acggacctgc tgcgtcccct cctcgaggag gcggagcggc
     9241 tggccgaccg ccgctacggg gtcgatgaga ccgaggacgt ctccctgcgg atcctggccg
     9301 agcacgggcg cgccacgacg ttcctgatcg ccgacggggt cgtcccgtcg aacgaggggc
     9361 gcgggtacgt cctccgccgc atgcttcgcc gcgtcgtgtc gcacgctcgc cggctgggcg
     9421 tggagggcgt ggtcctccgc ccgctggtgg agcggaccgt cgagatgctg ggcgacgcct
     9481 accccgaact cgtggagcgg cgggcgttcg tcctccaggt ggtctcctcg gaggaggagc
     9541 agttcgcccg gacgctcgcg cagggcatgg agctgttcga gcgcgaggcc gagcgggccc
     9601 gcgaggccgg agagcgggca ttccccgggg aggcggcgtt cctgtaccac gacacctacg
     9661 ggttcccgat cgagctcacc caagagctgg cccgagagca cgggctcgag gtggacgagg
     9721 ccgcgttcca gcgcctgatg gaggagcagc ggacgcgggc ccgccgggcc gcgaagggcg
     9781 gcgccgacga ccgggcgctg ctcgaggcgg cccgcgcggc cgggcgcact gagttcgtcg
     9841 gctacgcgga ggcggcggcc gaggggaagg tgctggcgct ggtccggaag ggcgaacggg
     9901 tcgaggcggg gggtgagggc gaggagatcg agctcctcct cgaccgcacg cccttctacg
     9961 cggagggggg agggcaggtc ggagacgcgg gggccatccg caccgagacg ggcgtggtgt
    10021 ccgtggccga caccgtgccc gggcccggcg aggtgatcgt gcaccggggg accgtgaacg
    10081 agggcgagat ccgggcgggc caggaggccc acctccagat cgaccttccc cgacgggagg
    10141 cgaccgcccg atcccacacc gggacgcacg tcctccactg gaccctccgg cacctcctgg
    10201 gcgagcacgc gcgccaggcg gggtcgctgg ttgccccggg gcgcctccgc tttgacttca
    10261 cccacttcga gggactctcg cccgatctgc tggaggagct cgaggccacc gcgaacgccc
    10321 ggctgggcga ggacgcctcc gtgcgggcct acgagaccac cttcgagtac gcgcgctccc
    10381 agggggctct ggcgctcttc ggtgagcagt acggcgacct cgtccgggtc gtggagatcg
    10441 gcgactactc ggtggagctg tgcggcggga cccacgtgtc gcacaccggc gaggtgtcgc
    10501 tcatggtgtt cggctccgag ggaagcatcg gctcggggct gcgcagggtc gaggccctcg
    10561 tcgggccgga cgcgctggcc cacgtgcacg cggagcgacg cctccttgag gagctcaccg
    10621 agatcctggg ggcccagggc ccccagcagg ccgtggagcg ggcgaggcgg gccgtggccc
    10681 gcatcaagga gctggagggc cagctcggcg agctccgcca gcaggagcgg ggggagctcg
    10741 tggacgcgct ccttcgggag acggccgacg tggggggcgt ctccctcgtg gtggcgcgcc
    10801 tcgacgagga ccccggcacg ctgcgggaga tggccctgaa gctccgggac cgcctccagg
    10861 gcgggcccgg agcggcggtc ctggcctcgg ccgatggaag gaaggcgacc ctggtggcgt
    10921 cggcgacgcc ggagctgatc gatcgagggg tgacggcgcc cgggctgctg gaggatgccg
    10981 cccgcgccgt cggaggcggc gcgggcggca agcaccacct cgcgttcgcc ggcggaggat
    11041 cgcccgacgc cctcgacgag gcgctccggg tcctgcccga ccgcctcctg gcgctcctcg
    11101 ggggatgacg gcgcccggcc gggtcttggc cctcgacgta gggaaggccc gggtcggggt
    11161 ggccgtctcg gaccctgacc gcacggtggc gctgcccgcg ggaacgatcc gcgtcgtagg
    11221 cgggatccag gacctccggg cggtggcccg gctcgtggag cggtacgagg cggtggtggt
    11281 ggtggtgggg caccccctct cgctgtcggg ggagcgggga cctgcggctc gccacgccga
    11341 ggagctggcc gacgggctcc gagcgatcct ggacgtgccg gtgcacctcc aggacgagcg
    11401 cctgagcacg atcgaggccg agcgggggct ggccgccgcc ggggcccgcg gtcccgaccg
    11461 gaggcgctcc gtggaccagg ccgcggccag catcatcctt cgcgcgttcc tggaccgcac
    11521 cgcgggctga cgggcggacg agcttcgata tcgaatatcc tgcggtgatg ctcgatcagg
    11581 tggggggagc gcaccgcgca cgggggcgcg cgctgagggt tctgctcgtc ctcttcctcg
    11641 tcctggccct tctgctggcc ggcgccttcg gcttctacgt ctgggccacc gggggctccg
    11701 ggccctcgcg cccggtcacc ctcgagatcc cccagggggc caccgtctcc gagatgggcg
    11761 ccgcgctcga ggaggccggc gtgatccgct ccgcgttcat gttcgggatc acggtcaggc
    11821 tgcagggcat cgggggcgag ctgaaggcgg gcacctacga caacatgcgg acgaacatgc
    11881 gcctggccga ggccctggac ctactgcagg ccgggccgcc ccccgagaag ctgccgctct
    11941 cagtgacggt ccccgagggc ctgcggatcg agcaggtggc cgaggtcctg gccgagggcc
    12001 tcggcatccg ccccagggct ttcctccggg aggcgaggag cggcgagtgg gcgatcgagc
    12061 cctggctccc cgaggggccc gagaccgtgg aggggttcct cttccccaag acctacgact
    12121 tcccggaggg cgttcgggcc cgggcggtac tgcgccggat gctggcccag ttcgaggagg
    12181 aggtctcggg cctggactgg tcgcgggccg aggcgctggg tctcgagccg tacgaggtgg
    12241 tcgtgatggc gtccctgatc gagcgcgagg cccgggtccc caaggatcgc aggaagatct
    12301 ccgcggtgat ccacaaccgc ctggagcggg acatgcccct ggagatcgac gccacggtcc
    12361 agtacgccct gcccgagcac aaggagcgcc tgaccttcga cgacctcgag tacgagagcc
    12421 cgtacaacac ctacctgttc gtcggcctgc cgcccggccc gatcgccagc cccggcgtgg
    12481 cctcgatcga ggccgcgatg aacccggcca acggcgactg gctctactat gtcctgatcg
    12541 acccggagac cggcgagcac gccttcgcgg agacctacga ggagttcctc gagtaccggg
    12601 agcaggccgg cttggggtga gcacggccct ctacgccgtc atggggtggc cggtggtgca
    12661 cagcgcttcg ccggcgatcc acacggcggc cttcgatacg ctggggatcc ccgccgtcta
    12721 cctgcgtctc ccggtgcctc ccggccgcgc cgccgcggcg atcgcctccc tctgggctct
    12781 gggggctcgc ggggcgagcg tcaccgcccc ccacaaggtc gcgatcgccg gcctctgtca
    12841 ggacctggcc cgctcggcgc gcaggacggg caccgtcaac accctcctga tcgaggaggg
    12901 atcggtcacg gggcacgaca ccgacgccga gggactgagg cggttcctgc tggaggacgc
    12961 gcgcctcgac ctcgccgggc tcaccgcgct cgtcctcggg ggcggcggcg ccgcgcgcgc
    13021 ctgcgcggtg gcgctgtcgg gtctggccgg gcgactggtg gtggccgcgc gcggcgccgc
    13081 cgccggcgcc gaggtggccg atctgttcga gggcccctcc accgtcatcc cgttcgtcga
    13141 ggcgggcgag gcccaggccg atctccttct caacgccacc cccctgggat ggcgcggcga
    13201 ggacgtcgcc gtcgaccctc gcccgggcca ggtggtcgtg gatctcgtct accgggagac
    13261 cccgctcctg cggcgggcgt gggctgtcgg agccgtcgcg cacgacggcc gggggatgct
    13321 ccttcgccag gcggctctct ccttccggct gtggacgggg gaggaccccc ctctggaggc
    13381 gatgcgggca gcgctcgacg gcgttctcac ctcggctgat tgacccgcgg gcctggttcc
    13441 ctcaaggtcc cgcacggagc cgccgatgtg aatggtgaca tgcctgggga gaccatcgac
    13501 tttgaggcgt tggccgcgcg cctctccgag atcgacggcg ttcgcgcgtg cctgctgatg
    13561 tcgcgtgacg gtctgacgct cggggtgcac ccggccgacc gagaggagtc ggcccgcgcc
    13621 gtctgggagg ggatgcactc gatcggcgac ccggagcgcg gattcctgac cgtgggcgac
    13681 gagctctgga tcgtcgcccg gcgaggtccg tacgcgggga tcgtcgtcgc cggaccctcg
    13741 gcaaccccag gcttgctgct cgaccggctg gagttctcac ttcgggcggc agaggagctg
    13801 aggctccgag agggttcggc ggcgacctcc ccccgtgcgg agggcagccg gaagccccgg
    13861 agccccctcc atcccgagac cgggctggcg ccgctgactc acgagcccga cgggtcgagc
    13921 gatgctgtct ccgccgcgat ccagatcgcc agcatgcgag agggccccat cacgctctcg
    13981 ccccgggacg ggaacggcca tcggaacggt tcgaagccac ccgagctcaa gctccggccg
    14041 ctggagacgg aggcccctct cgagcccgag cccgaccccg agcccgacgc cgagcccgct
    14101 cccgagcccg acgccgagcc cgctcccgag cgcggtctcg acatcgatct ggacgcgctg
    14161 ccggagcccg aggtcgagat cgagatccgc ccggttcccg acgaggagga gcccaccctg
    14221 gggtcgggcg agcacttccc cctccccgcc ggggacgagc gggaggtgga tccggtggcc
    14281 ctggcccgag agttcaagaa cctcttcgat cgccccgatg agtgaggggg atcgatcgag
    14341 agggatgaca tgccgagcga ggggacccga tgaggaagac caaggacctg ggtgaggtcc
    14401 tcgtcgacga gggcctgatc accgaggacg atctgggcaa ggcccacgag gagcaggagc
    14461 ggagcgggcg gtcgttgggc cgcgtgctcc tcgacctcaa cctcgtcgac gagaccaagc
    14521 tcgtcgaggc gcttgcccac cagatcgggc tccagttcgt cgacctctcc gagcacggcg
    14581 tggacgcctc cgccgtcgcg ctcgttcccg agcaggtggc ccgccgccac cgcgcgatcc
    14641 cgatcgcgtt cgaggactcc accctcgtcg tggcgatgtc cgacccgtcg aacgtgatcg
    14701 cgctggacga catccgcacg atcaccggct tcgaggtccg ggcggtcgtg gccacggcgg
    14761 ccgacgtgga cgaggcgatc aacaagtacg cgaagctcga ccagtccgtc gaggacatcg
    14821 cccatcaggc cgcgggcgcc gccgacgagg acttggatct cgatgcgatg gaggccgccg
    14881 tcgaggaggc cccgatcgtc aagctcgtca acctccttat cacccaagca gttgcggacc
    14941 gcgcctcgga catccacgtg gagcccgccg agaaggacgt gcgcatccgc taccggatcg
    15001 acggcgtgct ccacgaggtc atgcgctccc cgaagaacat ccagaacggc ctcgtctcg
//
LOCUS       DASUTZ010000037         8403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1434756, whole genome shotgun sequence.
ACCESSION   DASUTZ010000037 DASUTZ010000000
VERSION     DASUTZ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8403
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434756"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1309
                     /locus_tag="VF097_01665"
     CDS             170..1309
                     /locus_tag="VF097_01665"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6261534.1"
                     /translation="MSPLLLAYLAFGSFWGTWVVVFADFLRHHGLTPGRVSIWWTLLS
                     LVAILVMTFLAPRMEPLPRHGTVGWAMVAHAIGSALMVLTSTGWLPIAFVALGFGTGL
                     VDVFVNAAAYEIEQRSRSPVLQWVHAAYGAGGVVGSLGAGIAVTAGASFRAVVLGSVV
                     LQIGAAIAALRSGGLRATHTPREPGRVSLAVFKRARFLLVPALVVAAAFFIEGSMDVW
                     SVIFLRETLGASTLAGAAGFAAFAAAITIGRVVGAGSFRLGYARTILLSGLGSLGAGL
                     IAVATQSPLVASLAFLGLGLALASAAPAAYGMAEEAGPASGLAVGAITTVGYTGFVVG
                     PPIMGWLADAVSLRATMVALVVTTLGILAGGLLSFRRERAATPPG"
     gene            complement(1293..2732)
                     /locus_tag="VF097_01670"
     CDS             complement(1293..2732)
                     /locus_tag="VF097_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6261535.1"
                     /translation="MADELRNYVDGEWVDARDGGRFDVVDPATEEVIATAPDSQRADA
                     EAAISAARRAFDAGPWPRSAERERERVLLRAAEIVRREAERLAELETKDSGKPLAESR
                     EDIEEAAFMFEYYGGWATKVMGEIPPVGPDALSLVVKEPVGVAGLITPWNYPLLMAVQ
                     KVAPALAAGCTFVLKPAEQTPLTALELPGILEEAGVPPGVVNVVAGFGPSAGAPLVEH
                     PDVDKVGFTGSVEVGKEIMRTGADTLKRVTLELGGKSPNIIFADASFEEALEGTAAGI
                     FGNQGEICSAGSRIFVERSIYDDALEALRERARAVRLGSGLDEGTTMGPLVSREQQER
                     VLSYIEAGRSEGAKLATDEPPAFDSGYFVPPTVFYDVTNDMRIAREEIFGPVAAVLPF
                     EDPGEVVRLSNDTTYGLAAAVWTRDIKKALRTARALRAGVVWINDSQPAPTEAPWGGY
                     KQSGIGRELGPQGLEDYLETKHIYVNLAE"
     gene            complement(2732..3940)
                     /locus_tag="VF097_01675"
     CDS             complement(2732..3940)
                     /locus_tag="VF097_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020510981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX6261536.1"
                     /translation="MQAAPLDVAAVRAVTRPFHEARTLPAEAYLSPDVLAWELEVFFE
                     RSWMCVGRADDLREPGDQRAVAIGDEGILLVRDGDGALVAFHNTCRHRGHELLPCGGE
                     AVNRRVIQCPYHSWVYGLDGELRGAPWFADHPSFERSDPDNALRAARVESWGGWVFVN
                     ASGDAPPLAEHLGNLVELLDPYELDRLSSVEGHDYEIASNWKIVSENYHECYHCTEIH
                     PELCKVTPPDSGVQVEPTGLFLGGSMELLDHAETMSLTGESRGAPFRRLDEKLRREVL
                     YLQLMPNLLISAHPDYVMTHRLDPVAPDRTRIECRWLWAPESLELEGFDPSYASEFWD
                     ITNRQDWTACEAVQRGVAGRGFRQGPFSEMEGGVYQLAALVAQGYLAGKATGPVPTLP
                     AKAMGLTEGA"
     gene            complement(4010..4669)
                     /locus_tag="VF097_01680"
     CDS             complement(4010..4669)
                     /locus_tag="VF097_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6261537.1"
                     /translation="MNILIVEDEERIASFVEKGLKANGFSTSVAHDGERALSLHGTGD
                     FDLVILDLGLPGMHGLEVLGQLRGRDTRLPVIILTARDEAPDVVAGLDRGADDYVTKP
                     FRFDELLARVRARLRTDREPETTVLRVGDCVLDLRTRRVTVEDRTIELTAREFALAET
                     FFRHPGQVLSREQLLSNVWGYDYDPGSNVVDVYVGYLRKKLGGDRIATVRGMGYRLEA
                     G"
     gene            complement(4666..6135)
                     /locus_tag="VF097_01685"
     CDS             complement(4666..6135)
                     /locus_tag="VF097_01685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6261538.1"
                     /translation="MTEERAAATTPEADAPEPRGPRILWGVRARMLTWYVIVLAVAIG
                     AAVLVARQVLIAQLDERVHRELLQESDELRRLSRGRDPSTGEPFGPNVRRIFEVFLQR
                     NIPGRNETFLTIVGGQPFERSFREPPYRLDRDGELVARWSGLRRADRGRVTTPAGDVE
                     YLAVPVRSGGQTRGVFVAAIFREPELADIQRAISGTIGVGLVTLLIGSILAWRVIGGV
                     LGRVKRVTDTARDFSDDLTRRLDVTGNDEISHLALTFNGMLDRLEDLLETQRRFVDDA
                     GHELRTPITVIRGHLELLDDDPEERARTMALVTDELDRMQRIVNDLLVLAKAERPDFL
                     DLEPVPLDRLTEEIHAKAKALGERDWRLQQSAAGIVIGDRQRLTQAVMQLAQNAVQNT
                     QTGSEIAIGCSMQDGEARLWVRDSGPGIPADQRDRLFARFSRGVGRRRSEGAGLGLAI
                     VRAIAEAHHGRVEVESPPGSGATVSVVIPVDQPEEGRDA"
     gene            complement(6101..6436)
                     /locus_tag="VF097_01690"
     CDS             complement(6101..6436)
                     /locus_tag="VF097_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261539.1"
                     /translation="MSLRRALSVLLILALGFGVGWALIDLTRPEIRDDEIPVIIVREE
                     EEGDEAPRGGGGSGGGGGSGRGGGGGGDGGSGGGGAPPAPAPPPPPAGDDSDDGGTDD
                     GGAGGGDDT"
     gene            6564..7460
                     /locus_tag="VF097_01695"
     CDS             6564..7460
                     /locus_tag="VF097_01695"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX6261540.1"
                     /translation="MDVQERTKESWEFEEGQEIAPGLHALDPLGGGWRYEVYLAWDDR
                     LFTVVAAKLLRPDRAGERRAMDRFRVEAEAAQRLQHPVLPRCFGVDLEGEHPHIVLEY
                     MEGPRLSSLIRKQKALAIEQVIPLALQICSALHYMSVERVVHLDVKPKNIIMGAPPRL
                     IDLSIAAPLERAREIEGPIGTDAYMAPEQCEEGPHPRIDYRTDMWGLGVTMYESISGR
                     LPYPRGEEEAAGDDRFPQIVVEPEPLPREIPSVLADPVMSCLRHDPEERPTAAELAEI
                     LQPLADALPRNPRLGRRRPRLA"
     gene            7586..8005
                     /locus_tag="VF097_01700"
     CDS             7586..8005
                     /locus_tag="VF097_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261541.1"
                     /translation="MLAKVIIAAIAIALGGAVAWVTGPGDELAPVSGVEIQDDAKRPD
                     DGDDDDDDRGRGGDGDNTRGNDGTSGGNNTGDGDSTPGNNGTSGGNNTVRAATATNTN
                     GGGGGGGGGGTNTGGGTNTGGGTNTGGGSNSGGGSDT"
     gene            complement(8058..>8403)
                     /locus_tag="VF097_01705"
     CDS             complement(8058..>8403)
                     /locus_tag="VF097_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010041310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX6261542.1"
                     /translation="YRSVMVYGVPREVTDPEEKWEAQRALVDHVIPGRSADTRMPNEE
                     ELRQTTILALPLDEASAKVRMGPPKDEAEDLGLPFWAGEVPMRIVAGDPVPDPSLPEG
                     IEAPRYARRYRR"
BASE COUNT         1176 a         3076 c         2920 g         1231 t
ORIGIN      
        1 gagggccctg gaggcgggct gacggtcgag gaagatccgc tcctgctcct cgatcagctg
       61 atcgagacga tccatgctcg ctccttcatc tttgaacggc cattcaatat catacggacc
      121 gatcgaggag ggggcaatgg cgaggacggg agcgaggagc cgagcgggga tgagcccgct
      181 gctcctggcc tacctcgcgt tcgggtcctt ctgggggacc tgggtggtag tgttcgccga
      241 cttcctccgg caccacggcc tcacgccggg cagggtgagc atctggtgga ccctgctgtc
      301 gctcgtggcg atcctcgtga tgacgttcct ggcgccgcgc atggagccgc tcccccgcca
      361 cgggacggtc ggctgggcca tggtggcgca cgcgatcgga tcggccctca tggtcctgac
      421 ctccacggga tggctgccga tcgccttcgt ggcgctgggc ttcgggacgg gcctggtcga
      481 cgtcttcgtg aacgccgccg cctatgagat cgagcagcgc tcccggtctc ccgtcctcca
      541 gtgggtccac gccgcctacg gggccggcgg ggtggtgggg tcgctggggg ccgggatcgc
      601 cgtcacggcg ggcgcttcgt tccgggcggt cgtgctcggc tcggtggtgc tccagatcgg
      661 tgccgcgatc gccgcgcttc ggtccggagg gctgcgcgcg acccacaccc cgcgggagcc
      721 cggtcgcgtc tccctggccg tgttcaagag ggcccgcttc ctgctcgtcc ccgcgctcgt
      781 cgtggccgcg gccttcttca tcgagggctc gatggacgtc tggtcggtga tcttcctccg
      841 cgagacgctc ggggccagca cgctcgccgg cgcggcgggg ttcgccgcct tcgccgcggc
      901 gatcacgatc gggagggtcg tgggagcggg ctcgttccgg ctcgggtacg cccgcacgat
      961 cctgctttcg ggcttggggt cgctgggggc cggactgatc gcggtggcca cgcagagccc
     1021 gctggtggcg agcctggcct tcctcgggct cggcctcgcg ctcgcctcgg ccgccccggc
     1081 cgcgtacggg atggccgagg aggccggacc cgcgtcgggg ctcgccgtcg gggcgatcac
     1141 cacggtgggc tacacaggat tcgtcgtcgg gccgcccatc atgggctggc tcgccgacgc
     1201 ggtgagcctg cgcgcgacga tggtcgccct cgtcgtcacc acgctgggga tcctggccgg
     1261 agggctgctc tccttccggc gtgagcgcgc cgctactccg ccaggttgac gtagatgtgc
     1321 ttcgtctcga ggtagtcctc gagaccctgc ggcccgagct cgcggccgat ccccgactgc
     1381 ttgtagccac cccagggcgc ctccgtcggc gccggctggg agtcgttgat ccacaccacg
     1441 cccgcccgca gggctcgcgc ggttcgcagg gccttcttga tgtcacgggt ccagaccgcg
     1501 gccgccaacc cgtaggtggt gtcgttggag agccgcacga cctccccggg gtcctcgaac
     1561 ggcaggacgg ccgccaccgg cccgaagatc tcctcgcggg cgatccgcat gtcgttcgtg
     1621 acgtcgtaga agacggtcgg agggacgaag tagccgctgt cgaaggccgg cggctcgtcg
     1681 gtggcgagct tcgcgccctc gctcctgccc gcctcgatgt acgagagcac acgctcctgc
     1741 tgctccctgc tcacgagcgg ccccatcgtg gtgccctcgt ccagcccgct ccccagccgg
     1801 acggcacggg cccgctctcg aagtgcctcg agcgcgtcgt cgtagatcga gcgctccacg
     1861 aagatgcggc tcccagccga gcagatctcg ccctggttgc cgaagatgcc cgccgccgtg
     1921 ccctccagcg cctcctcgaa cgaggcgtcg gcgaagatga tgttcggcga cttcccgccg
     1981 agctccagcg tgacgcgctt cagcgtgtcg gcgcccgtcc gcatgatctc cttccccacc
     2041 tcgacggacc ccgtgaatcc gaccttgtcc acgtccggat gctcgacgag cggcgcaccg
     2101 gcgctggggc cgaagcccgc gaccacgttg accacaccgg gcgggacgcc ggcctcctcc
     2161 aggatgccgg ggagctccag cgcggtgagc ggcgtttgct cggcgggctt caggacgaag
     2221 gtgcacccgg ctgccagcgc cggtgcaacc ttctggacgg ccatgaggag ggggtagttc
     2281 cacggggtga tgaggccggc cacgccgacg ggctccttca ccacgaggct gagggcgtcc
     2341 gggccgacgg gcgggatctc gcccatcacc ttcgtggccc agccgccgta gtactcgaac
     2401 atgaaggcgg cctcctcgat gtcctcacgc gactcggcga ggggcttgcc ggagtccttc
     2461 gtctcgagct ccgccaggcg ctcggcctcg cggcggacga tctcggccgc ccgcaggagc
     2521 acgcgctcgc gctcgcgctc ggcggaccgg ggccaggggc ccgcgtcgaa ggccctgcgg
     2581 gcggccgaga tcgccgcctc cgcgtcggcc cgctgggagt cgggggccgt ggcgatgacc
     2641 tcctcggtgg cggggtccac cacgtcgaac ctcccgccgt cccgcgcgtc cacccactcg
     2701 ccgtcgacgt agttgcgaag ctcgtccgcc atcacgctcc ctccgtgagc cccatcgcct
     2761 tcgccggcag ggtgggcact gggcccgtgg ccttccccgc caggtacccc tgcgccacga
     2821 gcgccgcgag ctggtagacg ccaccctcca tctccgagaa cggcccctgc cggaacccgc
     2881 ggcccgccac gccgcgctgg accgcctcgc acgccgtcca gtcctgccgg ttcgtgatgt
     2941 cccagaactc cgaggcgtag gacggatcga acccctcgag ctccagcgac tcgggggccc
     3001 acagccagcg gcattcgatg cgcgtgcggt ccggagcgac cgggtccagg cggtgggtca
     3061 tgacatagtc ggggtgggcg ctgatcagca ggttcggcat cagctggagg tagaggacct
     3121 cccggcgcag cttctcgtcg aggcggcgga agggggctcc gcggctctcg cccgtcagcg
     3181 acatcgtctc ggcgtgatcg aggagctcca tcgacccccc caggaacagc ccggtcggct
     3241 cgacctggac cccgctgtcg ggcggggtga ccttgcacag ctcggggtgg atctcggtgc
     3301 agtggtagca ctcgtggtag ttctcggaaa cgatcttcca gttcgaggcg atctcgtagt
     3361 cgtggccctc cacggaggag agccggtcga gctcgtaggg gtcgagcaac tccacaaggt
     3421 ttcccaggtg ctccgccagg ggcggggcgt cgcccgaggc gttcacgaag acccagccgc
     3481 cccacgattc gacccgggcg gcccgcagag cgttgtccgg gtcgctccgc tcgaagctcg
     3541 ggtgatcggc gaaccacggc gcgccccgga gctcaccgtc cagcccgtat acccacgagt
     3601 ggtaggggca ctggatcacg cggcggttga cggcctcgcc cccgcacggg agcagctcgt
     3661 gccctcggtg gcggcaggtg ttgtggaagg cgaccagcgc gccgtcgccg tcccgaacca
     3721 acaggatgcc ctcgtccccg atggcgacgg cgcgctggtc gccgggctcg cggagatcgt
     3781 ccgcccgtcc gacgcacatc caggagcgct cgaagaacac ctcgagctcc caggccagca
     3841 cgtcgggcga gaggtaggcc tcggcgggca gcgtgcgggc ctcgtggaag ggccgggtca
     3901 ccgcccggac ggcggcgacg tcgaggggtg cggcctgcat ccggatcctc cgttctttga
     3961 ctcgccgttc aaacattcta gcgggggacc cggctcgacg cacccgccct cagccggcct
     4021 cgagacgata gcccatgccc cgcaccgtcg cgatccgatc gccccccagc ttcttccgca
     4081 ggtagccgac gtagacgtcc accacgttgg agccggggtc gtagtcgtac ccccacacgt
     4141 tgctcagcag ctgctccctg ctgaggacct gcccggggtg ccggaagaac gtctcggcca
     4201 gggcgaactc ccgtgccgtc agctcgatcg tgcggtcctc gaccgtcacc cgccgcgtcc
     4261 gcaggtcaag cacgcagtcc cccacccgga gcacggtcgt ctcgggctcc cggtctgtcc
     4321 ggagacgcgc ccggacccgc gccagcagct cgtcgaaccg gaagggcttg gtcacgtagt
     4381 cgtcggcccc ccggtcgagc ccggccacga cgtccggcgc ctcgtcccgg gcggtcagga
     4441 tgatcaccgg gaggcgcgtg tcccggccgc gcagctgtcc cagcacctcc aggccgtgca
     4501 tccccggcag ccccaggtcg aggatgacga ggtcgaagtc cccggtcccg tggagcgaca
     4561 gcgccctctc cccgtcgtgg gcgaccgagg tcgagaaccc gttcgccttc agccccttct
     4621 ccacgaacga ggcgatccgc tcctcgtcct cgacgatgag gatgttcacg cgtccctccc
     4681 ttcctccggc tggtccacgg ggatcacgac ggagaccgtg gccccggatc ccggagggct
     4741 ctcgacctcg accctcccgt ggtgggcctc cgcgatggcc cggacgatcg ccagccccag
     4801 cccggcgccc tccgaccggc gtctccccac gccccgcgag aagcgggcga acaggcgatc
     4861 ccgctggtcg gcggggatcc ccgggcccga gtcccgtacc cacaacctcg cctcgccatc
     4921 ctgcatcgag cagccgatcg cgatctcgga gcccgtctgg gtgttctgaa cggcgttctg
     4981 ggccagctgc atcaccgcct gggtcagccg ctggcgatcc ccgatcacga tccccgccgc
     5041 ggactgctgg agccgccagt cccgctctcc aagtgccttc gccttggcgt ggatctcctc
     5101 ggtgaggcgg tccagcggca ccggctccag gtcgaggaag tcgggccgct cggccttcgc
     5161 caggaccagc aggtcgttca cgatgcgctg catgcggtcg agctcgtcgg tgacgagggc
     5221 catggtgcgg gcccgctcct cggggtcgtc gtccagcagc tcgaggtgac cccggatcac
     5281 ggtgatcggc gtgcggagct cgtggccggc gtcgtccacg aaccggcgct gggtctcgag
     5341 caggtcctcg agacggtcga gcatcccgtt gaaggtcagg gcgaggtggg agatctcgtc
     5401 gttccccgtg acgtcaagac gtcgggtgag gtcatccgag aagtcccggg ccgtgtcggt
     5461 caccctcttc acgcggccca aaaccccgcc gatcacgcgc caggccagga tcgagccgat
     5521 caggagcgtc accaggccga cgccgatcgt ccccgagatg gctcgctgga tgtcggcgag
     5581 ctccggctcg cggaagatcg ccgccacgaa aacacccctg gtctgccctc ccgagcgcac
     5641 gggaacggcg aggtactcga catcgcccgc cggggtggtc acgcgcccgc ggtcggcccg
     5701 ccgcaggccg gaccagcgcg ccaccagctc gccatcgcga tccaaccggt aggggggctc
     5761 gcggaagctc cgctcgaagg gctggccccc cacgatcgtg aggaaggtct cgttccgccc
     5821 cgggatgttc cgctggagga acacctcgaa gatccgccgc acgttgggtc cgaagggctc
     5881 tcccgtgctc gggtccctgc ctcgggagag gcgccgcagc tcatcgctct cctgaagcag
     5941 ctcccggtgg acccgctcgt cgagctgcgc gatcagcacc tggcgagcca cgaggaccgc
     6001 ggcgccgatc gccaccgcga gaacgatgac gtaccaggtg agcatccggg cacggacgcc
     6061 ccagaggatc ctggggcccc ggggctcagg agcgtccgcc tcaggtgtcg tcgccgccgc
     6121 ccgctcctcc gtcatcggtg ccgccgtcgt cagagtcgtc tcccgccgga ggcggaggag
     6181 gcgctggggc cgggggcgcg ccgccaccgc ccgagccgcc gtcgccgccc ccgccaccac
     6241 cgcggcccga gccgccgccg ccccccgagc cgccgccgcc ccgaggggcc tcgtctccct
     6301 cctcctcctc gcgcacgatg atcaccggga tctcgtcgtc ccggatctcc gggcgggtca
     6361 ggtcgatcag ggcccagccc accccgaagc ccaaggcgag gatcaggagc acggacagcg
     6421 cacgtcgcag gctcatggct cgaaggatgg cggtcgcgca tcagccctcc atgagagacc
     6481 catgaggatg ttctcatgga gggctcacac ccggctcacg gctccggggg atggttgcct
     6541 cggatcaggt gggggaggat gccatggacg tgcaggaacg aaccaaggag agctgggagt
     6601 tcgaggaggg ccaggagatc gcccccggcc tccatgcgct ggatccgctg gggggcggat
     6661 ggcgctacga ggtctatctg gcgtgggacg atcggctctt caccgtggtc gcggcgaagc
     6721 tgctccgccc ggaccgcgcg ggcgagcgga gggcgatgga ccgcttccgc gtggaggccg
     6781 aggctgctca gcgcctccag catcccgtcc tcccccgctg cttcggcgtg gacctcgagg
     6841 gcgagcatcc ccacatcgtg ctggagtaca tggaggggcc ccgcctctcc agcctgatcc
     6901 ggaagcagaa ggccctcgcc atcgagcagg tcatccctct cgccctccag atctgctcgg
     6961 cgctccacta catgagcgtc gagcgggtgg tccacctcga cgtgaagccc aagaacatca
     7021 tcatgggcgc gccgcccagg ctgatcgatc tgagcatcgc cgcgccgctc gagcgggccc
     7081 gcgagatcga gggaccgatc ggaaccgacg cctacatggc gccggagcag tgcgaggagg
     7141 gaccgcaccc ccggatcgac taccgcaccg acatgtgggg gctgggggtc acgatgtacg
     7201 agtcgatctc cgggcggctc ccctaccccc ggggcgagga ggaggcggcc ggtgacgacc
     7261 gcttccccca gatcgtggtc gagccggagc cgctgccgcg ggagatccca tcggtgctgg
     7321 cggacccggt gatgtcgtgc cttcgccacg atcccgagga gcgccccacg gcggccgagc
     7381 tcgccgagat cctgcagccc ctggccgacg ccctcccccg gaaccctcgc ctggggcggc
     7441 ggcggccccg cctcgcctag gacgacgctc cgggaggcct cctcatccat gagacctccc
     7501 tcaccggggg cttaccgagg tctcaggacc gcgcgaaagg atgggtcgtg acagagatgg
     7561 gtacggacga cggaaggagg acgacatgtt ggcgaaggtg atcatcgcag ccatcgcgat
     7621 cgccctcgga ggcgccgtcg cgtgggtcac gggcccgggc gacgagctcg cccccgtctc
     7681 cggcgtggag atccaggacg acgcgaagcg cccggacgac ggagacgatg acgacgacga
     7741 ccgtgggcgg ggtggcgacg gcgacaacac ccgcggcaac gacggcacct ccggcgggaa
     7801 caacaccggg gacggcgaca gcacccccgg gaacaacggc acctccggtg ggaacaacac
     7861 cgtccgggcg gctaccgcga ccaacacgaa cggcggcggc ggtggtggtg gtggcggcgg
     7921 caccaacacc ggcgggggca cgaacaccgg cgggggcacg aacaccggcg ggggttcgaa
     7981 cagcggcggc ggcagcgaca cctgagtcgc accgatcgtc gaactgaagg ggcgcctccg
     8041 ggcgcccctt caccgtctca gcgccggtag cggcgggcgt accgcggcgc ctcgatcccc
     8101 tccggcagag aggggtcggg gacggggtcc ccggcgacga tccgcatggg cacctcgccg
     8161 gcccagaacg gcagacccag gtcctccgcc tcgtccttgg ggggccccat tcgcaccttc
     8221 gcggaggcct cgtccagcgg cagggccagg atcgtcgtct gccggagctc ctcctcgttc
     8281 ggcatccgcg tgtccgccga ccgtcccggg atcacatggt ccacgagcgc gcgctgagcc
     8341 tcccacttct cctccgggtc ggtgacctcc cgcgggaccc cgtacaccat cacggatcgg
     8401 tag
//
LOCUS       DASUTZ010000038         4420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1045928, whole genome shotgun sequence.
ACCESSION   DASUTZ010000038 DASUTZ010000000
VERSION     DASUTZ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045928"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      <1..114
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            163..1257
                     /gene="ribD"
                     /locus_tag="VF097_01710"
     CDS             163..1257
                     /gene="ribD"
                     /locus_tag="VF097_01710"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX6261543.1"
                     /translation="MDDAYMRRALELAERGRGLVSPNPLVGAIVVAGGEVVGEGWHEG
                     PGTDHAEIRALGAAGPRARGATLYVTLEPCSHHGRTPPCAPRVADAAPARVVAAITDP
                     NPLVDGAGLRLLRERGVEVTFGVLTEEASRQNRAFLRHVRTGLPFVTLKMAASLDGKA
                     AARDGSSRWITGAEARHDVHLLRASADAILVGAGTAFRDDPGLTVRLPSYRGAPKLRV
                     VVDGRGIVPETHRLFDGEAPTLVATTERAPRERREAWRRTADVLELEDAGDGLIPLDR
                     LMQELGKRDVQHVLVEGGPTLAWEMVERDVVDELILYVAPVLVGGEGAPSVLMGGGTP
                     SIGDGRGVTIREVSRVGADLKVVADVHRDR"
     gene            1238..1816
                     /locus_tag="VF097_01715"
     CDS             1238..1816
                     /locus_tag="VF097_01715"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX6261544.1"
                     /translation="MFTGIVEERGVVRASAPRLEVEAASVGADARTGESISVDGVCLT
                     VVDRIERTLAFDVAPETARRTTLGDLAEGDPVNLERPVTLATRLGGHLVQGHVDGVGE
                     ITSVSPEGDGARMTVRVPEGLGRYLVEKGSVTVDGVSLTVAEARPDGFAVALIPHTLE
                     ATGLGGKRPGDRVNLEMDLIAKHVERLIRRDR"
     gene            1813..3072
                     /locus_tag="VF097_01720"
     CDS             1813..3072
                     /locus_tag="VF097_01720"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEX6261545.1"
                     /translation="MKQEEDVGLVAGAFASIEEAVEDIAEGKIVIVVDDADRENEGDF
                     VIAAEKVTPEAINFMATHGRGLICLPSAGWRLDELGIPLMVQRSEGSQETAFAVSIDA
                     KGVTTTGTSAHDRAATCRAVTEPSTTPEDLLMPGHVFPLRAQEGGVLRRAGHTEAAVD
                     LASMAGLYPVGVICEIMHPDGTMARLPELLRVAREHDLKIISIADLIEHRRRREQLIE
                     MVAEATIPTIYGEFWAQVYESKIDGRQHVAMVLGQVGDGRDVMVRVHSECLTGDVFHS
                     LRCDCGTQLDSALGKIASEGRGVVLYIRGHEGRAIGLKHKLQAYSLQEQGKDTVEANL
                     ELGFPPDPRDYGVGAQILYDLGVRTMRLLTNNPSKRAGLEGHGLEIVERVPLETKPTA
                     QNLDYLRAKREKLGHLLESLEADGEGS"
     gene            3072..3548
                     /gene="ribH"
                     /locus_tag="VF097_01725"
     CDS             3072..3548
                     /gene="ribH"
                     /locus_tag="VF097_01725"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX6261546.1"
                     /translation="MAREGDLDARGRRFAVVVGRFNQVVTERLLEGARSAFRRHGVAD
                     EDVEVAWVPGSFEIPLVARRFARSGEVEAVVCLGAVIRGETAHFDVVTRESASGAQQV
                     ALETGVPCILGILTTDTLDQALERSGGKHGNKGWDAAVAAIEMASLMGSLPKERDA"
     gene            3545..3895
                     /locus_tag="VF097_01730"
     CDS             3545..3895
                     /locus_tag="VF097_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HEX6261547.1"
                     /translation="MIVDKPWGKVATYSLNQPSSVRILTILPGQETSEHYHQLRDEMW
                     IVLDPGLRIQIGNRVVDAQPGEEFVVPAEEPHRITNTTETRGRVLEVAHGYTTEDDTH
                     RLQDDYGRPLEPRW"
     gene            3975..4175
                     /locus_tag="VF097_01735"
     CDS             3975..4175
                     /locus_tag="VF097_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX6261548.1"
                     /translation="MTTGTVKWFSNEKGYGFIAQSDGDDVFVHFSAITGEGYKTLQEG
                     QEVEFEIVEGPKGKQAANVRPV"
BASE COUNT          676 a         1491 c         1659 g          594 t
ORIGIN      
        1 ggggtggaac tccccaccgg tggtgacagt ccacgacccc ccgcgagcca cgcgggggtg
       61 gacccggcgg aatcccggga ccgacggtga cagtccggat gagagaaggc gaggacggcg
      121 gcgtctgccg cccgttcccc cttcccccga gagaggaacg cggtggacga cgcgtacatg
      181 cggagggccc tcgagctggc cgagcgaggc cgcgggctcg tctcgcccaa ccccctcgtc
      241 ggcgccatcg tggtggccgg cggcgaggtc gttggggagg ggtggcacga aggccccggg
      301 accgaccacg ccgagatccg ggcgctcggg gccgccgggc cgcgggctcg cggcgcgacc
      361 ctctacgtga ccctggagcc gtgcagccat cacggccgca ccccgccctg cgcccctcgc
      421 gtggccgacg ccgccccggc cagggtcgtg gcggcgatca cggaccccaa ccccctcgtg
      481 gacggcgccg gccttcgcct cctccgggag cggggggtcg aggtgacgtt cggcgtcctg
      541 accgaggagg cctcgcggca gaaccgggcc ttcctgcggc acgtccggac cggcctcccg
      601 ttcgtgaccc tcaagatggc cgcttccttg gacgggaagg cggccgcgcg cgacgggtcc
      661 tcgaggtgga tcacgggggc ggaggcccgc cacgacgtgc acctcctccg ggcctcggcc
      721 gatgcgatcc tggtgggggc aggcacggcg ttccgggacg acccgggtct gaccgtcagg
      781 cttccctcgt accggggcgc ccccaagctc cgcgtggtgg tggacggccg ggggatcgtg
      841 ccagagacgc accggctgtt cgacggggag gcccccacgc tcgtggctac gaccgagcgt
      901 gccccaaggg agcggcgcga ggcgtggcgc cggacggccg acgtgctcga gctcgaggac
      961 gccggcgacg gcctgatccc gctggatcgg ctgatgcagg agctggggaa gcgagacgta
     1021 cagcacgtgc tcgtcgaggg cggccccacg ctggcctggg agatggtgga gcgagacgtc
     1081 gtggacgagc tgatcctgta cgtggccccg gtgctcgtgg gcggcgaggg agcccccagc
     1141 gtgctgatgg ggggcgggac gccctcgatc ggggacgggc ggggcgtgac gatccgagag
     1201 gtgtctcgcg tgggcgccga tctcaaggtg gtggcggatg ttcaccggga tcgttgagga
     1261 gcggggggtg gtgcgcgcgt ccgcgcctcg cctcgaggtc gaggccgcct cggtgggcgc
     1321 ggacgcgcgc accggcgagt cgatcagtgt ggacggtgtc tgcctgaccg tcgtggaccg
     1381 catcgagagg acgctggcct tcgacgtggc cccggagacc gcgcgccgga ccacgctcgg
     1441 agacctggcc gagggcgacc ccgtgaacct ggagcggccc gtgacgctcg ctacgcgcct
     1501 gggtggacac ctcgtccagg gccacgtgga cggcgtgggc gagatcacct cggtctcgcc
     1561 cgagggggac ggcgcccgga tgacggtgcg cgtcccggag ggcctgggcc ggtacctcgt
     1621 cgagaagggc tcggtgaccg tggacggcgt cagcctcacc gtggccgagg cgcggccgga
     1681 cgggtttgcc gtggcgctga tcccccacac cctcgaggcc accggcctgg gcggcaagcg
     1741 gccgggcgac cgggtcaacc tcgagatgga cctgatcgcg aagcacgtgg aacgactcat
     1801 ccggagggat cgatgaagca ggaggaggac gtcggcctgg tcgctggcgc gttcgcctcg
     1861 atcgaggagg cagtcgagga catcgccgag ggcaagatcg tcatcgtcgt cgacgacgcc
     1921 gaccgcgaga acgagggcga cttcgtgatc gcggccgaga aggtgacgcc cgaggcgatc
     1981 aacttcatgg ccacccacgg ccgcgggctg atctgtctcc ccagcgcggg gtggcggctc
     2041 gacgagctcg ggatcccgct catggtccag cgatcggagg gctcccagga gaccgcgttc
     2101 gccgtctcga tcgacgcaaa gggcgtgacg acgaccggga cgagcgcgca cgaccgcgcc
     2161 gcgacgtgcc gcgccgtcac cgagccttcc accacccccg aggacctcct gatgccgggg
     2221 cacgtcttcc cgcttcgggc ccaggagggc ggcgtcctcc gccgcgcggg gcacacggag
     2281 gccgcggtcg acctggcctc gatggccggc ctctaccccg tgggggtcat ctgcgagatc
     2341 atgcacccag acgggacgat ggcccgtctc cccgagctcc tccgggtggc ccgcgagcac
     2401 gacctgaaga tcatctcgat cgccgacctg atcgagcacc gcagacggcg ggagcagctg
     2461 atcgagatgg tggccgaggc caccatcccc acgatctacg gcgagttctg ggcccaggtc
     2521 tacgagtcga agatcgacgg tcgccagcac gtggccatgg tcctgggcca ggtcggcgac
     2581 gggcgcgatg tcatggtccg ggtccactcg gagtgcctca cgggcgacgt cttccacagc
     2641 ctgcgctgcg actgcggcac ccagctcgac tcggctctgg ggaagatcgc cagcgagggc
     2701 cggggtgtgg tgctgtacat ccgcggccac gaggggcggg ccatcggcct caagcacaag
     2761 ctccaggcct acagcctcca ggagcagggg aaggacaccg tcgaggcgaa cctcgagctc
     2821 ggcttcccgc ccgatccccg tgactacggg gtgggggcgc agatcctcta cgacctgggg
     2881 gttcgcacca tgcgcctgct caccaacaac ccctccaagc gggcggggct cgagggccac
     2941 gggctcgaga tcgtggagcg cgtgcccctg gagacgaagc ccacggccca gaacctcgac
     3001 tacctccggg cgaagcgcga gaagctgggg cacctgctgg agagcctcga ggccgacggg
     3061 gaggggtcgt gatggcgcgg gagggggacc tcgacgctcg cggccgccgg ttcgccgtgg
     3121 tcgtggggcg gttcaaccag gtggtgaccg agcggctcct ggagggcgcg cggtcggcct
     3181 tccgtcgtca cggcgtcgcc gacgaggacg tcgaggtggc ctgggtgccc gggtccttcg
     3241 agatccccct cgtggcccgc cggttcgcgc gctcgggcga ggtcgaggcg gtagtctgcc
     3301 tcggcgcggt catccggggg gagaccgcac acttcgacgt ggtgacgcgg gagtcggcca
     3361 gcggggccca gcaggttgcg ctggagaccg gcgtgccctg catcctcggc atcctcacga
     3421 ccgacacgct cgaccaggcg ctggagcggt cgggcggcaa gcacggcaac aaggggtggg
     3481 acgcggctgt ggccgcgatc gagatggcca gcctgatggg ctccctcccg aaggagcgtg
     3541 acgcatgatc gtggacaagc cctggggaaa ggtcgccacc tactccctca accagccctc
     3601 gtccgtcagg atcctcacga tcctgccggg ccaggagacc tccgagcact accaccagct
     3661 gagggacgag atgtggatcg tcctcgaccc gggcctgcgg atccagatcg gaaaccgcgt
     3721 ggtggacgcg cagccggggg aggagttcgt cgtccccgcc gaggagccgc accgcatcac
     3781 caacacgacc gagacccggg ggcgggtcct cgaggtggcc cacggctaca cgaccgagga
     3841 cgacacccac cggctccagg acgactacgg gagaccgctc gagccacggt ggtgacctgc
     3901 ggcttttcgg tcgctgtgct aggcttctgc gcgcatcatc ggcgggacgc gcccgcctgc
     3961 aaggaggtag ctcggtgacg accggaaccg tgaagtggtt cagcaacgag aaaggctacg
     4021 gtttcatcgc ccagtccgac ggggacgacg tcttcgtcca cttctccgcc atcaccggtg
     4081 aggggtacaa gaccctccag gaggggcagg aggtggagtt cgagatcgtc gaaggtccca
     4141 agggcaagca ggcggcgaac gtacggccgg tgtagctgca acccagacgc gggatcggga
     4201 gccccctcgg gggctcctcc ctttgtgcgg aggtatcgac agagcggacg agggaaccct
     4261 atactcgtga cagattgttc atccccggag gaaccgacgc gccgccgccg ttcgccggcg
     4321 cgtttcgtat ggaggtgatt cggcgtaccg gttgaacccc gcatcaacga gcggatccgc
     4381 gctccgcagg tccgcctggt aggacccgat ggcgggcaga
//
LOCUS       DASUTZ010000039         5008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_269125, whole genome shotgun sequence.
ACCESSION   DASUTZ010000039 DASUTZ010000000
VERSION     DASUTZ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269125"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..1408
                     /gene="ffh"
                     /locus_tag="VF097_01740"
     CDS             53..1408
                     /gene="ffh"
                     /locus_tag="VF097_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010649365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX6261549.1"
                     /translation="MFEQLTDRLGATFKKLRSRGKLHPKQVDKALGDIRDALLDADVA
                     VEVADSLLDRVRDRSLGEEVMKSVTPGQQVVKVVQDEMTATLGGEHRPFVLPDASPTV
                     IMMAGVQGSGKTTTCAKLAQHLGSKGRSVLLCAADLARPAAIEQLFQLGDEIGVPVVS
                     DGKDPVKVAKSALKQAKKESHDILIVDTAGRLHVDEEMMREVGRVRAALKPHHSLMAA
                     DAMTGQDAVRQAQAFMEHADLTGFVLTKVDGDARGGAALSITEVTGRPILFAGMGESP
                     GDLEPFHPDRMASRILGMGDVVSLVEKAQREMDEDAARESAEKMLKAQFSLEDFLTQL
                     REVKKLGPLEDLLAMLPGVPGGAGLKDLQVDENELVRAEAIISSMTLEERQNPALLNG
                     SRRSRVAKGSGTSVNQVNSLLKQYDEAKKMMKRLGGGGLGGLAKGLGGLGGMKLPKGL
                     G"
     gene            complement(1412..1729)
                     /locus_tag="VF097_01745"
     CDS             complement(1412..1729)
                     /locus_tag="VF097_01745"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX6261550.1"
                     /translation="MVQPDVLSLLPEHAKGAERPLTRVDLAVKVSSPSTRRTDTVRKR
                     DLYERHGVPEYWFVDLDADAILVYRLEEGRYGKPEILDPDEELTTPLLPGFSALVGDL
                     LLG"
     gene            complement(1666..1959)
                     /locus_tag="VF097_01750"
     CDS             complement(1666..1959)
                     /locus_tag="VF097_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX6261551.1"
                     /translation="MERRAMRLTYADLLERFPEETTQRIELIDGELLMPPAPSVRHQD
                     VVLEIGVRLRLAAERRGGLVVANPMDVLLTSTTWSSPTCCPSCRSTRRGRSGR"
     gene            2044..2571
                     /gene="rpsP"
                     /locus_tag="VF097_01755"
     CDS             2044..2571
                     /gene="rpsP"
                     /locus_tag="VF097_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX6261552.1"
                     /translation="MVKIRLMRMGAKKRPFFRVVVADSRSPRDGRFIENIGKYHPLEH
                     PSLIEIDEERAMHWLQNGAQPTQPVRVLLEKTGVWDKFQGGPGTPVPPPPDEEPAPPR
                     AEAEEAEEAEEAEAAPAEGGEAEAAAEPATEAELEPEAPSAEEAPAEEPQPEEAEEAQ
                     AEPEPQAEEGEEKPD"
     gene            2571..2834
                     /locus_tag="VF097_01760"
     CDS             2571..2834
                     /locus_tag="VF097_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX6261553.1"
                     /translation="MLEVVEYVAKFLVDEPDKVEAEEFEEDDGTVVIELTASPDDMGK
                     VIGKNGRVARALRQLVRAVGNREGVRATVDIVDELSEPEDDEE"
     gene            2848..3354
                     /gene="rimM"
                     /locus_tag="VF097_01765"
     CDS             2848..3354
                     /gene="rimM"
                     /locus_tag="VF097_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004600065.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX6261554.1"
                     /translation="MTPERGTDGLLAVGRVGKPHGLRGEVTVEPLTDTPDRFAPGATL
                     RMESGELTVAASRPHQRRMLVLFEGVEDRNQAEGLRGTILFVPDEDLPDLPDDAYWPR
                     DLVGCEVVTESGDRVGPISEVLRNPANDLWVTERGMIPAVREVVVEVDLEARRVVIRD
                     VPGLLEEA"
     gene            3356..4036
                     /gene="trmD"
                     /locus_tag="VF097_01770"
     CDS             3356..4036
                     /gene="trmD"
                     /locus_tag="VF097_01770"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX6261555.1"
                     /translation="MRIDVLTIFPAILESPLRESLLGKAIEAGILDVRVHDIRDHTTD
                     KHRQADDEPFGGGPGMVMKPEPIFAAAEAIGGAPRAILLSPAGRRLDQALVKELSQEP
                     HLLLIAGRYEGVDERVVEGLLAEEISIGDYVLSGGELPALVLIEAVTRLVPGVVGREE
                     SLDRESFQEEGLLDHPHYTRPREFRGMEVPEVLVSGNHAEIEAWRRRAAEEKTRRNRP
                     DLLEDPPG"
     gene            4086..4418
                     /gene="rplS"
                     /locus_tag="VF097_01775"
                     /pseudo
     CDS             4086..4418
                     /gene="rplS"
                     /locus_tag="VF097_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019854862.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
     gene            4658..>5008
                     /gene="lepB"
                     /locus_tag="VF097_01780"
     CDS             4658..>5008
                     /gene="lepB"
                     /locus_tag="VF097_01780"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011437965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX6261556.1"
                     /translation="MASPTERPEHGDRPTVPVAPARHRTDELSRDPADTPPDASEDEE
                     EEHHGPLHFLRELPGLILIALVLALLIKTFLVQAFYIPSESMVPTLRNGDRVLVNKLI
                     FNIRDPDRGEIVVFE"
BASE COUNT          807 a         1744 c         1800 g          657 t
ORIGIN      
        1 cctcgagctc gtggagcggc tcacctcctg acccccggct acactggggg cgatgttcga
       61 gcagctcacc gaccgcctcg gcgcgacctt caagaagctc cgctcccggg gcaagctcca
      121 tccgaagcag gtggacaagg ccctgggcga catccgcgac gccctcctcg acgccgacgt
      181 ggccgtggag gtcgcggact ccctgttgga ccgggtgcgg gatcgatcgt tgggcgagga
      241 ggtcatgaag agcgtcaccc ccggccagca ggtcgtgaag gtcgtgcagg acgagatgac
      301 agccacgcta gggggcgagc atcggccgtt cgtcctgccc gacgcgtcgc ccaccgtgat
      361 catgatggcc ggcgtccagg gctccgggaa gacgacgacc tgcgccaagc tggcccagca
      421 cctcgggagc aagggccgga gcgtcctcct gtgcgcggcc gacctggccc gcccggccgc
      481 gatcgagcag ctcttccagc tcggggacga gatcggggtc cccgtcgtct ccgacggcaa
      541 ggacccggtg aaggtggcca agagcgccct gaagcaggcg aagaaggaat cccacgacat
      601 cctcatcgtg gacaccgcgg ggcgtctcca cgtggacgag gagatgatgc gcgaggtggg
      661 ccgcgtccgg gcggcgctca agcctcacca ctcgctgatg gcggccgacg ccatgaccgg
      721 tcaggacgcc gtgcgccagg cccaggcctt catggagcac gccgacctca ccgggttcgt
      781 gctgacgaag gtggacggcg acgcccgcgg cggcgccgcc ctctccatca ccgaggtcac
      841 cggccgaccc atcctcttcg cggggatggg ggagagcccg ggtgacctcg agcccttcca
      901 ccccgaccgc atggcctcgc ggatcctcgg catgggcgac gtcgtctcgc tcgtggagaa
      961 ggctcagcgc gagatggacg aggacgccgc ccgcgagtcg gccgagaaga tgctgaaggc
     1021 ccagttctcg ctggaggact tcctgacgca gctccgcgag gtgaagaagc tggggccgct
     1081 cgaggacctg ctggcgatgc tgcccggcgt gcccggcggc gcgggcctca aggatctcca
     1141 ggtggacgag aacgagctcg tgcgggccga ggccatcatc tcctcgatga cgttggagga
     1201 gcgtcagaac ccggctctgc tgaacgggag ccgccgctcg cgcgtggcca aggggagcgg
     1261 caccagcgtg aatcaggtca actcgctgct gaagcagtac gacgaggcga agaagatgat
     1321 gaagcgcctc ggcggcggcg gtctcggcgg cctggccaag ggcctgggcg gcctcggggg
     1381 gatgaagctc cccaagggcc tcggctgagg ctcagcccag gagcaagtcg ccgaccaggg
     1441 ccgagaaccc gggcagcagc ggcgtcgtca gctcctcgtc ggggtccaag atctcgggct
     1501 tcccgtaccg gccctcctcc agccggtaga cgaggatcgc gtccgcgtcc aggtccacga
     1561 accagtactc gggcacgccg tgtcgctcgt agaggtcccg cttcctcacc gtgtcggtgc
     1621 ggcgggtgct cggcgaggac accttgacgg cgaggtccac tctcgtcagc ggccgctccg
     1681 cccccttcgc gtgctccggc aggagggaca gcacgtcggg ctggaccacg tcgtactcgt
     1741 cagcagcacg tccatcgggt tggcgaccac caggccgccc cgccgctccg cggcgaggcg
     1801 gagtcgcacg ccgatctcca ggacgacgtc ctggtggcga acgctcggcg cggggggcat
     1861 cagcagctcg ccgtcgatga gctcgatgcg ctgggtcgtc tcctcgggga accgctccag
     1921 gagatccgcg taggtgagcc gcatcgctcg tcgctccaca ggtcgaaacg ctatcctgag
     1981 cctacgacac gaacccgcgc gggggtgccc tcgcgtccgg gcgaccgatc aggagacgta
     2041 cgcatggtga agatcaggct gatgcggatg ggcgcgaaga agcggccgtt cttccgcgtg
     2101 gtggtggccg actcgcgcag cccgcgagac ggccgcttca tcgagaacat cgggaagtac
     2161 cacccgctgg agcatccgtc gctcatcgag atcgacgagg agcgggcgat gcactggctg
     2221 cagaacggcg cccagcccac ccagcccgtc cgggtcctgc tggagaagac cggcgtctgg
     2281 gacaagttcc agggcggccc cggcacgccg gtcccgccgc ctcccgacga ggagccggcg
     2341 ccgcctcgcg cggaggctga ggaggccgag gaggccgagg aggccgaggc agcgcccgcc
     2401 gagggagggg aagcggaggc cgcggcagag cccgccactg aggccgagct cgagcccgag
     2461 gcgccgagcg cggaggaagc tccggcggag gagcctcagc ctgaggaggc cgaggaagcc
     2521 caggccgagc ccgagcccca ggccgaggaa ggcgaggaga agcccgactg atgctcgagg
     2581 tggtggagta cgtcgccaag ttcctcgtgg acgagccgga caaggtcgag gccgaggagt
     2641 tcgaggagga cgacggcacc gtcgtgatcg agctcactgc ctcccccgac gacatgggga
     2701 aggtgatcgg caagaacggc cgcgtggcgc gtgcccttcg ccagctcgtc cgggccgtcg
     2761 ggaatcgaga gggcgtccgg gcgaccgtcg acatcgtcga cgagctctcg gagcccgagg
     2821 acgacgagga gtagcccagg cggggccatg accccggagc gcgggaccga cgggctcctg
     2881 gcggtgggcc gcgtcggcaa gccccacggc ctccgcggcg aggtcaccgt cgagccgctt
     2941 accgacaccc ccgatcggtt cgcccccggc gccaccctgc gcatggagtc gggggagctc
     3001 accgtcgccg cctcccgtcc ccaccagcgc aggatgctcg tcctcttcga aggggtggag
     3061 gatcggaacc aggcggaagg gctccgcggc acgatcctct tcgtccccga cgaggacctg
     3121 cccgatctcc ccgacgacgc ctactggcct cgggacctcg tcggatgcga ggtcgtcacg
     3181 gagtccggcg accgagtcgg ccccatcagc gaggtcctcc gcaaccccgc caacgacctc
     3241 tgggtgaccg agcgggggat gatcccggcg gtgagggagg tcgtggtcga ggtggacctc
     3301 gaggcgcgac gcgtggtgat ccgcgacgtt cccggcctgc tcgaggaggc ctgaggtgcg
     3361 gatcgacgtc ctgacgatct tccccgcgat cttggagagc ccgctgcgcg agagcctgct
     3421 gggcaaggcg atcgaagcgg ggatcctcga cgtgagggtc catgacatcc gcgaccacac
     3481 gaccgacaaa caccggcagg ccgacgacga gccgttcggc ggcgggccgg ggatggtgat
     3541 gaagcccgag cctatcttcg cggcggcgga ggcgatcggc ggcgccccgc gggcgatcct
     3601 gctgtcgccg gcggggcgcc gcctcgacca ggcactcgtg aaggagctct cgcaggagcc
     3661 ccacctcctg cttatcgccg gtcgctacga gggtgtggac gagcgtgtgg tcgagggcct
     3721 cctcgccgag gagatctcca tcggcgacta cgttctctcg ggcggcgagc tgccggcgct
     3781 cgtcctgatc gaggccgtca cccggctcgt ccccggcgtc gtgggacgcg aggagtcgct
     3841 ggatcgcgag tccttccagg aggaggggct cctcgatcac cctcactaca cgcgaccccg
     3901 ggaattccgg gggatggagg tgcccgaggt gctcgtgtcc gggaaccacg ccgagatcga
     3961 ggcgtggcgc cgccgcgccg cggaggagaa gacccgccgc aaccgccccg acctcctcga
     4021 agaccccccg ggatagacgc cgtcgcgtag tatcattggc gcccgcgcga cctgtaggag
     4081 ttcccgtgaa caagaccgat ctcgtcgaga agccgcgcct gcgcagggac gtcccgaact
     4141 tccgccccgg cgacaccgtc aaggtgctcg tccgcgtcgt ggaggccggc cgcgagcgcg
     4201 tccaggcgtt ccagggcgtc gtcctccgcc gcaagggcgg gggcctccgc gagaccttca
     4261 ccgtccgcaa ggtgtccttc ggggtcgggg tcgagcgcac cttccccgtg cactcgccct
     4321 ccatctccaa gatcgaggtt gtctcgcgcg gcgacgtccg tcgggccaag ctctactacc
     4381 tgcgcgagcg gcgcgggaag cgtgcccgga tccgcgagct ccgcggggcc gcggccgact
     4441 cgctggcggc cgaggagtcc gccagggttg ccgagctcga ggccgaggag cgcgagcgag
     4501 ccgagaccga ggcggccgag gcgcggctgg cggaggaggc ggaggccgca gaggccgcga
     4561 agaccgagga gcctcaggcc gaggagcccc aggccgagcc cgaggccgag cgggccgagc
     4621 ccgaggccga cgccccggag aaggccgaag cctgacggtg gcttctccca ccgagcgtcc
     4681 cgagcacggg gaccggccga ccgtccccgt cgcccccgcc cgccaccgca ccgacgagct
     4741 gtcccgcgac cccgcggaca ctcccccgga cgcctcggaa gacgaggaag aggagcatca
     4801 tggcccactc cacttcctga gggagcttcc ggggctcatc ctgatcgccc tcgtcctggc
     4861 gctcctcatc aagacgttcc tcgtccaggc cttctacatc ccatctgagt cgatggtccc
     4921 cacgctgcgc aacggcgacc gagtgctcgt caacaagctg atcttcaaca tccgtgaccc
     4981 ggatcgaggg gagatcgtcg tcttcgag
//
LOCUS       DASUTZ010000040        20307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1565138, whole genome shotgun sequence.
ACCESSION   DASUTZ010000040 DASUTZ010000000
VERSION     DASUTZ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 20307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20307
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565138"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..1230
                     /locus_tag="VF097_01785"
     CDS             13..1230
                     /locus_tag="VF097_01785"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX6261557.1"
                     /translation="MLQLDQAAQIMDLPANVHEILKHPKRILQVAIPVQMDDGSLRVF
                     TGFRVHHNTSRGPSKGGIRYHPDVSLDEVKALAMWMTWKCALVGIPYGGAKGGVIVNP
                     KELSRKELERMTRRYASEILPLIGPERDIPAPDMGTDDQIMAWIMDTYSMNQGYSVPG
                     VVTGKPVSVGGSLGRGTATARGVVYVTVATLKHLGFPVDQSRVVIQGYGKVGGPVVRL
                     LHDQGCRVVGVSDVMGGVYNPRGLSPMALASYQEETRTVSGFPGGDSIASDEVLEVEA
                     DVVIPAALEGVITEDNAERIQAKVVVEAANGPTTPEADEILHDRGILVVPDILANAGG
                     VTVSYFEWVQDIQAYFWSEDEVNERLREIMESAHVQVIQLAEEKKATMRQAATMIGVQ
                     RVAEAIETRGLFP"
     gene            1305..1553
                     /locus_tag="VF097_01790"
     CDS             1305..1553
                     /locus_tag="VF097_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HEX6261558.1"
                     /translation="MEEPLDERSRAILDFERSWWQLPGRKEAAIRRRFGFSAPRYYRL
                     LNRLIDTPEALRYDPMLVKRLRRLRAERRRSRFERTMG"
     gene            1561..2121
                     /locus_tag="VF097_01795"
     CDS             1561..2121
                     /locus_tag="VF097_01795"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HEX6261559.1"
                     /translation="MAGKHEPDAKRSFYLSLATATLRAGLVIAAVVLGIFVLSRAFPG
                     AGDESQGPGPVTTDGAEESPGPEESPQEDGGGGGGGGGQAPQEVALRGVTVNVQNGTG
                     VTGLAADTAEELRGLGLRIREIGDAARHFDVTTLYFAQGSRPEAEELNATFFNGEANV
                     QRMPAELEDDVQISVVLGQDYADAQG"
     gene            complement(2118..2933)
                     /locus_tag="VF097_01800"
     CDS             complement(2118..2933)
                     /locus_tag="VF097_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261560.1"
                     /translation="MRRLASLLLVASILLPACRDPHVSLVYRLEDDTHLRYSLRLIAD
                     VERTLQDETRQERVEAVFRASQDVLETFPDGGGRVGVTLDPVSLTVNGEPQSAGPAQD
                     FVVVVSPGGEIEEIEETAEAGPAPLAPVGVDRILLRLRPVLPEGPVTPGDAWTSGAVL
                     TDEDGSFSLEAHSRLAALGRLDGRGAALVRTTYTSPVDRREVFANAVADLMGRDVGTQ
                     EAWFSLEGFLVRARGDSVGRYAVTFRPPGGEDDIEPVQGRLRVRLRTEMRLLP"
     gene            complement(2944..4377)
                     /locus_tag="VF097_01805"
     CDS             complement(2944..4377)
                     /locus_tag="VF097_01805"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX6261561.1"
                     /translation="MSPGILVASNRGPVSFVEEDGRLRATRGGGGLVTALTGVVHETE
                     GMWIAAAMSEGDRRRIEGVPHGRIEVVLEDEKYRLRLLSFDPETYERFYNTVSNRLLW
                     FLHHYLWDVPRWPRFGTPSRISWDAYRTVNLAFAEALAEEAGESGATALIQDYHLSLV
                     PAQLRARAPETKVAYFHHCPFAGPGYMRLVPTWLREEMLDGLLGADLVGFQSARWAED
                     FLFSARMLPDAEIDLERRTVTWRGRETKVGVYPITVDPEPMREHAASQAVETKRQKLA
                     EWRGDRKLLLRVDRAELSKNILRGFLAFEDLLQRHPEWRERVVFLAHITPSRTALPEY
                     QTYTQQCIDVATRINDEMGTPGWIPIQIDMSDDLDQAYAAYGLYDVLLVNPVFDGMNL
                     VAKEGPLLNEGHGVLVLSRNAGAIAELDRWALPVDPVDIAGTADALHAALEMPDEERR
                     ARARGLRAAAEARTPADWIGEQLRDLG"
     gene            complement(4374..5621)
                     /locus_tag="VF097_01810"
     CDS             complement(4374..5621)
                     /locus_tag="VF097_01810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6261562.1"
                     /translation="MLISVPVQETRSLDDYIPSAGEDSVSRLREAAEPLRGARLVHLS
                     STAFGGGVAELLPTQIAIMRDLGMDVDWQLLEGSDDFFAVTKLAHNALQGADMPWNET
                     MASTYLERVHANAERWGDDADFVIVHDPQPLAIASFVEERGKRSGRWVWRCHIDLSKP
                     MEEVWAFYSRFLGSYDASIFTLEDYVHEGADSNAHLIPPSIDPLSPKNHDLEDEVVEE
                     VLTSYEVDPGRPIVTQVSRFDPWKDPLGVMDAFRLAREHVPEVQLVMVGSMAHDDPEG
                     WHYLELAEEHRGGDPDIHLLTNLQDVGALAVNAFQRASTVVMQKSIREGFGLTCTEGM
                     WKERAVIAGRVGGLRLQIEDGHSGFLVDTIEEAADRLVELLRDREAREGMGKAARERV
                     RHRFLSTREVDDHIRLLASLGTR"
     gene            complement(5643..6071)
                     /locus_tag="VF097_01815"
     CDS             complement(5643..6071)
                     /locus_tag="VF097_01815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6261563.1"
                     /translation="MRVLIIDDEPDVILLCRVNLEFEGHEVLEAASGEEGLEIAEKEL
                     PDLIVLDLMLPRQDGFSVLRHLHSHEPTKHIPVVLLTAKSREDDQMRGWQTGADEYVT
                     KPFSPVALTEAVARVAGMTAEEREARRRERLEQLSLLHRL"
     gene            6180..7493
                     /locus_tag="VF097_01820"
     CDS             6180..7493
                     /locus_tag="VF097_01820"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX6261564.1"
                     /translation="MAEERKGPLWTPGVSRAARWGIIAWSAIGVLLILYWAFRYVIHP
                     VRVIFPPLVLALIVVYLLNPIVSWLEGHGIPRVIGALLTYLVLLGALGIGLRYLIPVL
                     VEQAQDFSRSLPSLLGRAQQAITDVLQRFGAEGDPGGLLDSGQVQAVTERLFNITLGL
                     LNGAIILVIGLILGVYLLIDIPKIQRGIVALVPERRRPEVLTIGEKVGRAIGGFFRGQ
                     LLVALFVGVLSTLGLYIVGLPYWALVGMITGLFNIIPLIGPFIGGAIAVFVAFASDAP
                     GEGLLQLEAGWPLAVGSVIALVAVQQLDNHIVSPNVVARTVKMHPVTVMLALLAGGTL
                     LGLWGMLLAVPVFASVKILVLHAWDTKMTWPPTPPPTDVEVESSAPLPEPDPPHPSPS
                     PRPTSRFSSWVDRWRSGRRRAADSGRPEEAPSEEEPASKGAEESL"
     gene            complement(7490..8326)
                     /locus_tag="VF097_01825"
     CDS             complement(7490..8326)
                     /locus_tag="VF097_01825"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEX6261565.1"
                     /translation="MRRAGDVLRRARRPRVIYTDLDGTLLGPGGSLFADPQGGVSNAA
                     ANALVALYGAGIDVVPVSGRTAEQVWEVARVIGASDFVAEMGGILSLDRDAARIRQDG
                     AHRGASTPYEAMVEAGAPGMLLESFPRRLEPHAPWAFLPRVTSMLFRGHLDTGEAEAT
                     LEEAGYGWLTLLDNGVIPRSFPGLDVEEVHAYHLLPRGVTKATGVGAHVERRGLHPEE
                     CLAVGDSLSDAALAETVGSVVIVANGREAVEASGRAPDGLLATEASFGEGFAEVVAEV
                     LA"
     gene            complement(8323..9282)
                     /locus_tag="VF097_01830"
     CDS             complement(8323..9282)
                     /locus_tag="VF097_01830"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HEX6261566.1"
                     /translation="MIAERWFARRTFHHSRFDDVEGLVEAKERQEISISACIPTLNEA
                     ETIGPIVSGIHRELIERLPLLDELVVMDSASEDGTARIAEEEGATVVQDAEVLSGLEQ
                     ASGKGEALWKSLFVLKGDLIVWLDADIQNFHPRFVYGPLGPLLADPEIAYTKAFYDRP
                     IKVGDHLQLAEGGRVTELAARPLLNMFWPELAAVVQPLSGEYAGRRSILEQLPFFTGY
                     GVEIGLLIDIAARYGVDSIAQVDLDVRVHRNQDIAALSRMAFAVLQAAMRRLEAAGRI
                     TLSEELGTSYLQLQGTTEHILERRELRLAERPPAVTIPGYASR"
     gene            9356..9829
                     /locus_tag="VF097_01835"
     CDS             9356..9829
                     /locus_tag="VF097_01835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX6261567.1"
                     /translation="MLKISQKTEYAIRAVVELALRAGQGPVPAREIADAQGIPLRFLE
                     QQLSALAKAGLIESLRGAGGGARLAKDPSQVRVADIVDAIEGPFFPMYCLDPSDHTCF
                     QDSRCGIQGLWGEVQAAVRVVFERMTVADLALRHRHIAPESAMWSPEDLLRPSRS"
     gene            complement(9842..9994)
                     /locus_tag="VF097_01840"
     CDS             complement(9842..9994)
                     /locus_tag="VF097_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020525294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HEX6261568.1"
                     /translation="MATYEGYCVKDKKKVEFEGEVVTLKNGRRAAKGKCPLCGTTVMR
                     ILGKND"
     gene            10183..11805
                     /gene="groL"
                     /locus_tag="VF097_01845"
     CDS             10183..11805
                     /gene="groL"
                     /locus_tag="VF097_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012180551.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX6261569.1"
                     /translation="MAAKIISFDEQARRALERGMDQLANAVRITLGPKGRNVVLEKKW
                     GAPTITNDGVSIAKEIELDDPEESVGAELVKEVAKKTNDVAGDGTTTATVLAHAMVKE
                     GLRNVAAGANPMGLKRGIETAVERAVEAIKNQAREIEAKEEIAHVAAISAGDPEIGEI
                     IAEAMDKVGKDGVVTVEESQTFGMELDLVEGMRFDKGYISAYFVTDPERMEVVLEEPY
                     VLLANQKISAVKDLLPILEKVMQSGKPLVIIAEDVEGEALATLVVNKIRGTFKSAAVK
                     APGFGDRRKAMLQDIAILTGGQVVSEEVGLKLENAQLDILGQARKIVITKDETTIVEG
                     AGSDDEIKGRINQIKNEIDATDSDYDREKLQERLAKLSGGVAVIKVGAATEVELKEKK
                     HRIEDAVSATKAAVEEGVVPGGGVALLAAQEALDKVDLEGDEATGARLVRHALEAPLK
                     QIAENAGLEGGVVVEKVRALDAGQGLNAAIGEYEDMMKAGIIDPAKVTRSALQNAASI
                     AALFLTTEALVAEKPEKQPPMPAMPDAGGMDF"
     gene            11894..12124
                     /locus_tag="VF097_01850"
     CDS             11894..12124
                     /locus_tag="VF097_01850"
                     /inference="COORDINATES: protein motif:HMM:NF020940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HEX6261570.1"
                     /translation="MTRRFHLTFPGHLAGEPVLFLVGKEAGVTPNIRRASVEEGGAWM
                     IVELDGGNEKISDAVARLTDRGVVVRRVDEDE"
     gene            12124..12492
                     /locus_tag="VF097_01855"
     CDS             12124..12492
                     /locus_tag="VF097_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6261571.1"
                     /translation="MAKKRILLIDHRPEHMRQPVLRLQLEGYEVDEASDGKTGIEKLR
                     ARRFDLLLLDAELPGSDGWETLRAIREDEELTDLKVIIFMAGKGETGKLHLLPVDAEL
                     RRPFPIGELLAKVHRVLGDA"
     gene            complement(12497..13495)
                     /gene="galT"
                     /locus_tag="VF097_01860"
     CDS             complement(12497..13495)
                     /gene="galT"
                     /locus_tag="VF097_01860"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEX6261572.1"
                     /translation="MSTLRQDPTTAEWVIIAPHRAARPRAAASVERPAGLPFDADCPF
                     CPGNEERTPPEIARTERDGSWDQRVFANLYPALQGNGSTERQGNALFREMPGVGQHEV
                     IVESPRHDERLDDMETERLARALGVWRDRYTALRERPWVKSVIVFRNFGARAGTSLPH
                     PHSQILGTPVFAPDALRRYSVATRYFDDTGHCVYDDLREAELGARDRVVFENETFVAL
                     APFASRNPYETWILPRRHRPSFGDVPDDELEGLAQALSRVLRALREGAGDPDYNLIVQ
                     SAPAHEKGKPFFLWHLQLFPRTTTPAGFELGSGMSINPMAPEQAAATLREAIAVRP"
     gene            13572..14390
                     /locus_tag="VF097_01865"
     CDS             13572..14390
                     /locus_tag="VF097_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6261573.1"
                     /translation="MVIDVSERDFEERVLKASREVPVIVDFWAGWCRPCLVLGPILEG
                     LAEEHEGRFVLAKLDVDANQALAARYGVQGIPAVKAFRDGEVVSEFVGAQPEDVVRLW
                     LREVLPSEAEPIVAEAVGLEEEGDLERAEGGYRRALEIDPDHEEATVGLARVLLARGE
                     AAEAGRLASRLPGSVEARAIAARAALVERIPGDGDLDDLRVRAESDPEAALAVAAADA
                     EQGQTSEALDRLLGLVGGETRDRARDLMVTIFESLGDDHPLVREYRPKLASALY"
     gene            14390..14728
                     /locus_tag="VF097_01870"
     CDS             14390..14728
                     /locus_tag="VF097_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HEX6261574.1"
                     /translation="MAEVTVEEFQRIDVRAGTVVRCERFPEARSPAYKLWIDLGPQIG
                     ERRSSARITDHYEPEELVGRQVAAVVNFPPRRIGPFSSEVLVLGFYDEDGTVVLVGPD
                     RSVPNGARLG"
     gene            14731..15135
                     /locus_tag="VF097_01875"
     CDS             14731..15135
                     /locus_tag="VF097_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HEX6261575.1"
                     /translation="MESSRFSVDTTGRLVTDLTERVQDFVRDRGGEGLLNVFLPHATC
                     GLALMETGSGSERDLQDALDGLLPPEDRYSHRHGSTGHGRDHLLPLFISPSLTLPVEG
                     GEVVLGTWQSIVLVDTNRENNRRDVVLSLLRG"
     gene            15160..16404
                     /locus_tag="VF097_01880"
     CDS             15160..16404
                     /locus_tag="VF097_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261576.1"
                     /translation="MTERDGTQGLLDRGRAFFTWASRPMRRMWTSDDPLEDYALVHMA
                     SVAGDTLLAIALADSIFFALPVGEARTQVALYLGLTMAPLAVAGPLLVPLLDRSPFRR
                     AISFGASVGRAVAVLLAARWLDSLLLFPAVFTVLVLTRIHLIAKNGLTVAYAPTGAAL
                     MRANAFLARTAIAGALLAAVPGVVMLQLGRTRGVLVLAALVYAAAALLNLRLAPVERH
                     PPRAGEVPRRGRIPALAEAALGAGGLRGAQGFLMALLAFALRDAGAPTYWFGVLLVTG
                     TAGAAIADFLVPRLPTTVREEVIVVGSLLAAGLGAFLASQAYGLPSLALFVGVAGMST
                     EAGRLAFQSLMQANAPGGAQGRVFVRYEVAFQLAWVMGAFIPAMLPMSFRGGFIALAP
                     FYLALGVVYVVRLVRARRLRAG"
     gene            16466..16900
                     /locus_tag="VF097_01885"
     CDS             16466..16900
                     /locus_tag="VF097_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261577.1"
                     /translation="MGRTLICLVLLASGCTGTEPPEAGPTGTPPAEQIQRESHLVAGH
                     DRPELPCLEAGPDDPPPRLEMGDTFFAPSCVVLRGRHDLRLANGGALEHSFGVRDAGV
                     SVDLEPGASGEVSLEDLRGSGSTEFFCRFHASAGMTGTITVA"
     gene            16951..17616
                     /locus_tag="VF097_01890"
     CDS             16951..17616
                     /locus_tag="VF097_01890"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEX6261578.1"
                     /translation="MSDEGPRIEVTQNGNYRVRGRVPIYPGRILRSEDGSSIDWELDE
                     PYPLRRTVTLCRCGRSGNKPFCDDTHQTIEWDGEETADRGPTSARRKAVPGQGVTLTD
                     DVSLCTSAAFCHDRVTDVWRLTFHRSDEPEVRRRIEGIVARCPSGRLVLETHDGAEVE
                     PEYEPSIVVQEGGPYWVRGRVPIMAVDGRTWEVRNRLALCRCGASGNKPFCDSTHLEI
                     GFA"
     gene            17724..18161
                     /locus_tag="VF097_01895"
     CDS             17724..18161
                     /locus_tag="VF097_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261579.1"
                     /translation="MAGVTGSGGALARRRTISAVSLALVTMLMVALPAVPASAAKARV
                     KAKVGANKWVKVHTYIGKGDVVIWKNPRPKMVSKRRVHDVTSIQPRRWKKRLGFKLSK
                     VLQPGQRVKRKFRRTGTYRYRCARHSAMVDGKCQGMCGFIHVL"
     gene            18487..18918
                     /locus_tag="VF097_01900"
     CDS             18487..18918
                     /locus_tag="VF097_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010059393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HEX6261580.1"
                     /translation="MATEKRADVTWRGDLATGRGTIERVGSGVIEGLGVSWAARTGES
                     DDLTSPEELIAAAHASCFSMALSKILADQGHAPDRLLVTATVSFVPGTGITRSALDVT
                     GVVPGMEEAEFGNAAEAAKENCPVSQALKGNVELTVASRLS"
     gene            18986..19225
                     /locus_tag="VF097_01905"
     CDS             18986..19225
                     /locus_tag="VF097_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261581.1"
                     /translation="MSLIRTRLISLVTVVIVALGVPSLASAAPGGDIREACGASFGQL
                     VSAGRSSGTAFHTNYAGGAMAFSDPAVLAAHGCTL"
     gene            complement(19272..>20307)
                     /locus_tag="VF097_01910"
     CDS             complement(19272..>20307)
                     /locus_tag="VF097_01910"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020646767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX6261582.1"
                     /translation="GFAYAVWDFLDVPPGGTINPDRGTFGGGLGFKGGSLISRTTDGG
                     MTWSTPRRLYNPGGVNQTIGNQIVVRPDGTLVNVFNEILNFRNDDRDAQFDFNLALKV
                     SPDAGRTWLPKGPPIRFGDMLPRTLFNPVFPFVSVYQPDADGLETLDGANAVRSGEVI
                     PEVAVDPNNGNLYVVWMDARFTLPGATTVLDLIDEVAFTMSTDGGFTWTTPIKVNQTP
                     ENIRLGNRQAFTPMVRVLDDGTVAVSYYDFRNNVDDDGVGTTDMFVAHCHPASEDCAN
                     EASWDEETQVTTSSFDITEAPFANGFFLGDYVGLETDGTDFLSLFTQSGVGGDSASEF
                     FSRIGLGKIG"
BASE COUNT         3029 a         6888 c         7262 g         3128 t
ORIGIN      
        1 tggcggcagg cgctgctgca gctcgatcag gccgcccaga tcatggacct gcccgccaac
       61 gttcacgaga tcctgaagca ccccaagcgc atcctgcagg tcgccatccc ggtccagatg
      121 gacgatggga gcctccgcgt cttcacgggg ttccgcgtcc accacaacac ctcgcgtggg
      181 ccatccaagg gagggatccg gtaccacccg gacgtctcgc tggacgaggt gaaggccctc
      241 gcgatgtgga tgacctggaa gtgcgcgctc gtcggcatcc cctatggggg cgccaagggg
      301 ggcgtgatcg tgaaccccaa ggagctctct cgcaaggagc tcgagcgcat gacccgccgc
      361 tacgcgtccg agatccttcc gctgatcggt cccgagcgcg acatcccggc cccggacatg
      421 ggaaccgacg accagatcat ggcctggatc atggacacct actcgatgaa ccaggggtac
      481 tcggtcccgg gcgtggtgac gggcaagccc gtgtccgtgg ggggctcgct ggggaggggc
      541 acggcgacgg cgcgcggggt cgtctacgtc acggtggcca ccctgaagca cctcggcttc
      601 cccgtggatc agtcgcgggt cgtgatccag gggtacggga aggtgggggg gccggtcgtc
      661 cgcctgctcc acgaccaggg gtgccgggtc gtcggggtct ccgacgtgat gggcggcgtg
      721 tacaaccccc ggggcctctc cccgatggcc ctggcgagct accaggagga gacgcggacg
      781 gtctccggct tccccggggg ggactcgatc gccagcgacg aggtcctgga ggtcgaggcc
      841 gacgtcgtga tccccgccgc gctggagggg gtcatcaccg aagacaacgc cgagcgcatc
      901 caggccaagg tcgtggtgga ggccgccaac gggccgacca cgcccgaggc cgacgagatc
      961 ctccacgacc gaggcatcct cgtcgtcccc gacatcctcg ccaacgccgg gggtgtcacc
     1021 gtctcctact tcgagtgggt gcaggatatc caggcgtact tctggtccga ggacgaggtc
     1081 aacgagcggc ttcgcgagat catggaatcg gcccacgtcc aggtgatcca gctggccgag
     1141 gagaagaagg ccacgatgcg gcaggcggcc acgatgatcg gtgtgcaaag ggtggccgag
     1201 gcgatcgaga cccgcggcct cttcccgtga catgtccttg aacggcccat atctcgtgcc
     1261 tatgctcgga cgcgacacaa gatgtagcga ccgagaggag gcgcatggag gagccgctgg
     1321 acgagcggtc ccgggccatc ctggacttcg agcggtcctg gtggcagctc cctggccgca
     1381 aggaggccgc gatccggcgc cgattcggat tctcggcccc gcggtattat cgtctgctga
     1441 atcggctgat cgacacacca gaggcgcttc ggtacgaccc gatgctggtg aagcgcctgc
     1501 ggcggctccg agcggagcgc cgtcgctcgc ggttcgagcg caccatgggg tgaggagcgt
     1561 gtggccggca agcatgaacc cgacgcgaaa aggagcttct acctctcctt ggcgaccgcc
     1621 acgctgagag ccgggctcgt catcgccgcg gtcgtcctcg gcatcttcgt cctgtccagg
     1681 gcgttcccgg gcgccggaga cgagtcacag ggccccggcc cggtcaccac tgacggggcc
     1741 gaggagagcc caggccccga ggagtcgccc caagaggacg gcggcggggg tggcggcggc
     1801 ggcgggcagg cgccccagga ggtcgccctt cggggtgtga ccgtcaacgt ccagaacggc
     1861 acgggcgtga cggggctcgc cgcggacacc gccgaggagc tgcggggcct cggcctgcgc
     1921 atccgggaga tcggggacgc cgcccgccac ttcgacgtga cgaccctcta cttcgcccag
     1981 ggctcgcggc ccgaggccga ggagctgaac gcgacgttct tcaacgggga ggcgaacgtc
     2041 cagcgcatgc cggcggagct cgaggacgac gtccagatca gtgtcgtcct cggtcaggac
     2101 tacgccgacg ctcagggcta gggcaggagg cgcatctccg tccgcaggcg cacacggagc
     2161 cgcccctgca ccggctcgat gtcatcctcc cctcccggcg gccggaacgt caccgcatac
     2221 cgacccactg agtcaccccg cgcccgaacc aggaacccct ccagcgagaa ccacgcctcc
     2281 tgggtcccga cgtcgcgacc catgaggtcg gcgaccgcgt tggcgaagac ctcccgtcgg
     2341 tcgaccggcg aggtgtaggt ggtccgcacg agcgcggccc cccgtccatc cagccggccc
     2401 agagcggcga gcctcgaatg ggcctcgagt gagaagctgc cgtcctcgtc tgtcaggacc
     2461 gcgcccgacg tccaggcgtc gcctggggtc acgggcccct cggggagaac gggccgcagg
     2521 cgcagcagga tccgatcgac ccccactggg gccaggggcg ctggcccggc ctcggccgtc
     2581 tcctcgatct cctcgatctc cccgcccggg ctcaccacga cgacgaagtc ctgtgccggg
     2641 ccggcgctct gaggctcacc gttcaccgtg agcgagaccg gatcgagtgt cacgccgacc
     2701 cggcccccgc cgtcggggaa ggtctcgagg acatcctggc tggcccggaa caccgcctcc
     2761 acccgctcct gcctggtctc gtcctggagc gtccgctcga cgtcggcgat cagacggagc
     2821 gagtaccgga gatgcgtgtc gtcctcgagc cggtacacga gcgagacgtg cgggtcccgg
     2881 caggcgggga ggaggatcga ggcgacgagg agaagggaag cgaggcgacg cacggcggcc
     2941 atcctagccg aggtcgcgga gctgctcgcc gatccagtcg gcgggcgttc gggcctcggc
     3001 cgccgcccgc aggccccggg cccgcgcccg acgctcctcg tcgggcatct cgagcgccgc
     3061 gtgcagggcg tctgccgtgc ccgcgatgtc cacgggatcc accgggaggg cccagcgatc
     3121 cagctccgcg atcgcccccg cgttgcgcga gagcacgagc accccgtggc cttcgttcag
     3181 caacggccct tccttggcca caaggttcat gccgtcgaag accgggttca ccaggaggac
     3241 gtcgtagagg ccgtaggcag cgtaggcctg gtcgaggtcg tcgctcatgt cgatctggat
     3301 ggggatccag cccggcgttc ccatctcgtc gttgatccgg gtcgccacgt cgatgcactg
     3361 ctgcgtgtac gtctggtatt cggggagggc cgtgcgagac ggcgtgatgt gagccaggaa
     3421 caccacgcgc tcccgccact cggggtgccg ctggaggagg tcctcgaacg ccaggaaccc
     3481 acgcaggatg ttcttggaga gctcggcccg gtccacgcga aggaggagct tccggtcccc
     3541 gcgccactcg gcgagcttct gtcgcttcgt ctcgacggcc tgggaggccg cgtgctcgcg
     3601 catcggctcg gggtccaccg tgatcgggta cacgccgacc ttcgtctctc gtcctctcca
     3661 ggtcacggtt cgccgctcga ggtcgatctc ggcgtccggc agcatgcggg ccgagaagag
     3721 gaagtcctcg gcccaccgcg cgctctgaaa ccctacgagg tccgcgccca ggagcccgtc
     3781 gagcatctcc tctcggagcc aggtcggcac gagcctcatg tagccgggtc cggcgaaggg
     3841 gcagtggtgg aagtaggcga ccttcgtctc gggcgccctg gcccggagct gggccgggac
     3901 gagcgagagg tggtagtcct ggatcagcgc cgtggcgccg ctctccccgg cttcctcggc
     3961 cagcgcctcg gcgaaggcca ggttcaccgt ccggtaggcg tcccaggaga tccgagacgg
     4021 ggtgccgaat cgaggccacc gcgggacgtc ccacaggtag tggtggagga accacaggag
     4081 ccggttgctc accgtgttgt agaagcgctc gtaggtctcc ggatcgaacg agaggagccg
     4141 gaggcggtac ttctcatcct ccagcaccac ctcgatgcgg ccgtggggga cgccctcgat
     4201 cctcctacgg tccccctcgc tcatggcggc ggcgatccac atcccctcgg tctcgtggac
     4261 cacgccggtg agcgcggtga cgagtccacc gcctcctcgc gtggcccgga gccggccgtc
     4321 ctcctcgacg aagctgaccg gaccgcggtt cgaggcgacg aggatgccgg ggctcaccgg
     4381 gtccccagcg aggcgagcag gcggatgtga tcgtccacct ctcgcgtgga gaggaagcgg
     4441 tgacgcaccc gctcccgcgc cgccttcccc atgccctcgc gggcctcccg atcccggagc
     4501 agctcgacga gccgatcggc cgcctcctcg atcgtgtcca ccaggaagcc gctgtggccg
     4561 tcctcgatct ggaggcggag gccccccacc cgcccggcga tcaccgcccg ctccttccac
     4621 atgccctcgg tgcacgtgag gccgaagccc tcgcggatcg acttctgcat cacaacggtc
     4681 gaggcgcgct ggaacgcgtt gacggccaag gcccccacgt cctggaggtt cgtgagcagg
     4741 tggatgtcgg ggtccccgcc gcggtgctct tcggcgagct cgaggtagtg ccagccctcg
     4801 gggtcgtcgt gcgccatcga gcccaccatc acgagctgga cctcggggac atgctcacgt
     4861 gccagccgga aggcgtccat cacgccgagg gggtccttcc aggggtcgaa gcgggagacc
     4921 tgcgtgacga tgggcctgcc ggggtcgacc tcatagctcg tgaggacctc ctcgaccacc
     4981 tcgtcctcga ggtcgtggtt cttgggtgag aggggatcga tcgacggggg gatcaggtgc
     5041 gcgttggagt cggctccttc gtgcacgtag tcctcgaggg tgaagatgct cgcgtcatag
     5101 ctacccagga accgggagta gaaggcccag acctcctcca tcggcttgga caggtcgatg
     5161 tgacagcgcc acacccatcg tccgctcctc ttcccccgct cctcgacgaa ggacgcgatc
     5221 gcgagcggct gggggtcgtg gacgatcacg aagtcggcgt cgtcgcccca ccgctcggcg
     5281 ttggcgtgca cgcgctcgag gtaggtcgac gccatggtct cgttccaggg catgtcggcc
     5341 ccctgcagcg cgttgtgggc gagcttggtg accgcgaaga agtcgtctga cccctcgagg
     5401 agctgccagt ccacgtccat gccgaggtcc cgcatgatcg cgatctgggt gggcaggagc
     5461 tcggctaccc cgccgccgaa ggcggtgctg ctgaggtgga cgagtcgggc cccgcggagc
     5521 ggctcggcgg cctcccggag gcgcgatacg gagtcttcgc cggccgaggg gatgtagtcg
     5581 tcgagggagc gcgtctcctg aaccgggacg gagatcagca tcgcggccca gtctaacggg
     5641 cctcagagac ggtggagcag gctcagctgc tcgaggcgct cgcggcgtct ggcctctcgt
     5701 tcctcagcgg tcatcccggc caccctggcc acggcctcgg tgagggcgac tgggctgaac
     5761 ggcttcgtca cgtactcgtc ggcgcccgtc tgccacccac gcatctggtc gtcctcgcgg
     5821 gacttggccg tgaggaggac cacggggatg tgcttcgtcg gctcgtgcga gtgtaggtgt
     5881 cgtagcacgg agaacccgtc ctgccggggg agcatgaggt cgaggacgat gaggtcgggg
     5941 agctccttct ccgcgatctc gaggccctcc tcgccgctcg cggcctcgag cacctcgtgc
     6001 ccctcgaact ccaggttcac gcggcacagg aggatcacgt ccggttcgtc gtcgatgatg
     6061 agcacgcgca ccgcgtcacc tccttcccat ccatcggcgc ccgggggggc accgcttgag
     6121 ccacgcggtc acacgctcgg gcggttcggg agaggagccg acggtatgat gacgcccgga
     6181 tggccgagga gcggaagggc ccgttgtgga cgcccggagt ctcccgcgcg gcacgctggg
     6241 ggatcatcgc ctggtcggcc atcggcgtcc tcctgatcct ctactgggcg ttccgatacg
     6301 tgatccaccc cgtgcgggtg atcttcccgc ccctcgtcct cgccttgatc gtggtctacc
     6361 tgctcaaccc gatcgtctcc tggctggagg ggcatggcat cccgcgggtg atcggggccc
     6421 tgctcaccta cctcgtgctg ctcggggcgc tggggatcgg tcttcggtat ctgatccccg
     6481 tgctcgtgga gcaggcccag gacttctctc gatccctgcc gagcctgctg ggtcgggctc
     6541 agcaggcgat caccgacgtc ctccagcggt tcggggcgga gggggatccc ggtggcctgc
     6601 tggacagcgg acaggtccag gcggtgacgg agcgcctctt caacatcacc cttggcctgc
     6661 tcaacggggc gatcatcctc gtgatcgggc tgatcctcgg cgtctacctc ctgatcgaca
     6721 tccccaagat ccagcgtggg atcgtggcgt tggtgccgga gcgccggcgg ccggaggtgc
     6781 tcacgatcgg tgagaaggtg ggtcgagcga tcggggggtt cttccgtggg cagctgctcg
     6841 tggccctctt cgtcggcgtc ctctcgaccc tcggcctcta catcgtgggg ctgccgtact
     6901 gggcgctcgt cgggatgatc accggcctct ttaacatcat cccgttgatc ggccccttca
     6961 tcggaggggc gatcgccgtc ttcgtggctt tcgcctccga cgccccgggg gagggcctcc
     7021 tgcagctcga ggcgggttgg ccgctggcgg tcggatcggt gatcgccctg gtcgccgtcc
     7081 agcagctcga caaccacatc gtcagcccga acgtcgtggc ccgcacggtg aagatgcacc
     7141 ccgtgacggt gatgctggcc ctgctcgccg gcggcaccct cctggggctc tgggggatgt
     7201 tgctcgcggt gccggtcttc gcgtcggtca agatcctggt cctccacgca tgggacacga
     7261 agatgacctg gccaccgacg ccgcccccga cggacgtcga ggtggagtcg tcggcgccgc
     7321 tcccggagcc ggaccccccg cacccgtcgc cgtctccgcg cccaacttcg cggttctcct
     7381 cgtgggtcga ccgatggcgt tcgggacgcc ggagggccgc ggactccggg cgaccggagg
     7441 aggccccgag cgaggaggag cccgcctcga agggcgctga ggagagtctc taggcgagga
     7501 cctccgccac gacctcggcg aacccctcgc cgaaggaagc ctcggtggcc agcaggccgt
     7561 ccggggcccg gcccgaagcc tcgacggcct cccggccgtt ggccacgatg accacgcttc
     7621 caaccgtctc cgcgagcgcc gcgtcgctga gggagtcacc cacggcgagg cactcctccg
     7681 ggtggagacc tcgccgctcg acgtgggccc ccacgcccgt ggccttcgtc acgccccggg
     7741 gaaggaggtg gtaggcgtgg acctcctcca cgtccagccc ggggaaggac cgggggatga
     7801 ccccgttgtc gaggagggtg agccatccgt acccggcctc ctcgagcgtc gcctccgcct
     7861 cgccggtgtc caggtggccc cggaagagca tggaggtcac tcgcggcagg aaggcccagg
     7921 gagcgtgggg ctcgagccgc cgagggaagg actcgaggag catcccgggc gcgcccgcct
     7981 cgaccatcgc ctcgtatggc gtcgatgcgc cgcgatgagc cccgtcctgg cggatccgcg
     8041 cggcgtcccg atcgagcgag aggatccccc ccatctcggc gacgaagtcg ctcgcgccga
     8101 tcacccgggc gacctcccac acctgctcgg cggtgcggcc cgagaccggg accacgtcga
     8161 tgcccgcccc gtacagggcg acgagggcgt tcgctgccgc gttcgacaca ccgccctggg
     8221 ggtcggcgaa gagcgagcct cccggaccga gcagggtccc gtcgaggtcg gtgtagatca
     8281 cccgggggcg gcgcgcccgc cggaggacgt ctcccgccct tctcatctcg aggcgtagcc
     8341 cgggatcgtg acggccggtg gacgctctgc gagacgcagc tccctccgct cgaggatgtg
     8401 ctccgtggtc ccctgcagct gtaggtagga ggtgccgagc tcctcggaca gggtgatcct
     8461 gcccgcggcc tcgagtcggc gcatcgcggc ctggaggacg gcgaaggcca tcctcgagag
     8521 cgcggcgatg tcctggttgc ggtggactcg gacgtcgaga tccacctggg cgatcgagtc
     8581 caccccgtag cgagccgcga tgtcgatcag caggccgatc tcgacgccgt agccggtgaa
     8641 gaagggcagc tgctcgagga tgctgcgccg acccgcgtac tcccccgaca ggggctggac
     8701 gaccgcggcg agctcgggcc agaacatatt gaggaggggg cgggcggcga gctccgtgac
     8761 ccgacccccc tcggccaact gcaggtggtc gcccaccttg atcggccggt cgtagaacgc
     8821 cttcgtatag gcgatctccg ggtcggcgag gagcgggccg agcggcccgt acacgaagcg
     8881 gggatggaag ttctggatgt cggcgtccag ccagacgatg aggtctccct tgaggacgaa
     8941 gaggctcttc cacagggcct cgcccttgcc gcttgcctgc tcgagcccgg aaaggacctc
     9001 cgcgtcctgc actacggtgg cgccttcctc ctccgcgatc ctggccgtcc cgtcctcaga
     9061 cgcggaatcc atcacgacca gctcgtccag gagcgggagg cgctcgatga gctcgcggtg
     9121 gatcccggat acgatcggtc cgatcgtctc ggcctcgttc agggtgggga tgcaggccga
     9181 gatcgagatc tcctggcgct ccttcgcttc caccaagccc tcgacgtcgt cgaaccgcga
     9241 gtggtggaag gtgcggcgag cgaaccagcg ctcagcgatc accgctcgat gatatcggcc
     9301 cgcgaggggc cgccgccccc atcgaatccg accgctgcga tatgattcat gacggatgct
     9361 caagatctcc cagaagaccg agtacgcgat ccgagcggtg gtggagctcg cgcttcgggc
     9421 gggccagggc cccgtcccgg cccgcgagat cgccgacgcc cagggcatcc ctctccgctt
     9481 cctcgagcag cagctctcgg cgctggccaa ggctggcctg atcgagtcgc tgaggggcgc
     9541 ggggggcggc gcgcggctgg ccaaggaccc ctcccaggtc cgcgtggccg acatcgtgga
     9601 cgcgatcgag gggccgttct tcccgatgta ctgcctcgac ccgtccgatc acacgtgctt
     9661 ccaggactct cgctgcggga tccaggggct gtggggagag gtccaggcgg cggttcgcgt
     9721 ggtgttcgag cggatgaccg tcgcggacct tgcgctgaga caccggcaca tcgcgcccga
     9781 gagcgcgatg tggtcgccgg aggacctgct caggccgagc aggagctagg gggttcgggc
     9841 gtcagtcgtt cttgcccagg atgcgcatca ccgtcgtccc gcagaggggg cacttaccct
     9901 tggcggcccg ccgtccgttc ttgagggtca cgacctcgcc ctcgaactcg accttcttct
     9961 tgtccttgac gcagtacccc tcgtaggtcg ccaccggcgc ctccttccga acagtcttca
    10021 gggccgctcc gtgcggcccc tgcgtggaat cgtggcccct cggggagcgg gttgcaagca
    10081 tctcccccct tgtgctcccc gggcgcggcg cgtgtagaat acggcttagc actcgaagcc
    10141 agagagtgcc agcgttcgac cacacggact caggaggaac ggatggccgc gaagatcatc
    10201 tcgttcgacg agcaggcacg gcgcgccctg gagcgcggca tggaccagct cgcgaacgcc
    10261 gtacggatca cgctgggccc caaggggcgc aacgtggtcc tggagaagaa gtggggcgcc
    10321 cccacgatca cgaacgacgg cgtctccatc gccaaggaga tcgagctcga cgaccccgag
    10381 gagagcgtcg gcgccgagct ggtgaaggag gtcgccaaga agaccaacga cgtggcaggg
    10441 gacggcacga cgaccgccac ggtgctcgcc cacgccatgg tgaaggaggg cctgcgcaac
    10501 gtggccgccg gtgccaatcc gatgggcctc aagcggggca tcgagacggc ggtcgagcgt
    10561 gccgtggagg cgatcaagaa ccaggcccgt gagatcgagg ccaaggagga gatcgcacac
    10621 gtcgccgcga tctcggccgg agacccggag atcggcgaga tcatcgccga ggcgatggac
    10681 aaggtgggga aggacggcgt ggtgacggtc gaggagtccc agaccttcgg gatggagctc
    10741 gacctcgtcg agggcatgcg gttcgacaag ggctacatct ccgcctactt cgtgaccgac
    10801 cccgagcgga tggaggtcgt cctcgaggag ccgtacgtcc tgctggcgaa ccagaagatc
    10861 tccgccgtga aggacctgct cccgatcctg gagaaggtga tgcagtccgg gaagcctctg
    10921 gtgatcatcg ccgaggacgt cgagggcgag gctctggcca cgctcgtggt gaacaagatc
    10981 cggggcacct tcaagtccgc agcggtgaag gcgcccgggt tcggtgaccg ccggaaggcg
    11041 atgctgcagg acatcgcgat cctcaccggc gggcaggtcg tctccgagga ggtcgggctg
    11101 aagctcgaga acgcccagct cgacatcctg ggccaggctc ggaagatcgt catcaccaag
    11161 gacgagacga cgatcgtcga gggagccggt tccgacgacg agatcaaggg ccggatcaac
    11221 cagatcaaga acgagatcga cgcaaccgac tccgactacg accgcgagaa gctccaggag
    11281 cggctggcca agctctccgg gggcgtggcc gtgatcaagg tcggggcggc gacggaggtc
    11341 gagctgaagg agaagaagca ccgcatcgag gacgcggtct cggccacgaa ggcggcggtc
    11401 gaggagggtg tcgtccccgg cggcggcgtc gctctgttgg cggcgcagga ggctctggac
    11461 aaggtggacc tcgagggcga cgaggccacc ggggccaggc tggtccgcca cgcgctcgag
    11521 gcgccgctga agcagatcgc cgagaacgcc ggcctcgagg gcggtgtggt ggtcgagaag
    11581 gtgcgcgccc tcgacgccgg tcagggcctg aacgcggcca tcggcgagta cgaggacatg
    11641 atgaaggccg ggatcatcga cccggccaag gtcacgcgct cggccctcca gaacgcggcg
    11701 tcgatcgcgg cgctgttcct caccaccgag gcgctggtgg cggagaagcc cgagaagcag
    11761 cctccgatgc cggcgatgcc ggacgcgggg ggcatggatt tctgagggtg gacgaaggag
    11821 aggcccccgc cgaaaggcgg gggcctctcc ttttcggcgt gtgcgggccg ccggcatggg
    11881 agacaatccg agggtgacgc ggcgcttcca cctgactttc cccggccacc tggccggcga
    11941 gccggtgctc ttcctggtgg ggaaggaagc tggggtcacg cccaacatcc gccgggccag
    12001 cgtggaggag ggaggcgcct ggatgatcgt ggagctcgac ggtggcaacg agaagatctc
    12061 ggatgcggtg gcccgcctga ccgaccgggg cgtcgtcgtg cgtcgtgtgg acgaggacga
    12121 gtgatggcga agaaacgcat cctgctgatc gatcaccggc ccgagcacat gcgccagccg
    12181 gtgctgcgcc tgcagctcga gggctacgag gtggacgagg catccgacgg caagaccggc
    12241 atcgagaagc tccgggcccg ccgcttcgac ctcctcctcc ttgacgcgga gctcccgggc
    12301 agcgacggat gggagacgct gcgcgcgatc cgcgaggacg aggagctcac cgacctcaag
    12361 gtcatcatct tcatggcggg caagggggag acggggaagc tccacctgct gccggtcgac
    12421 gccgagctgc gcaggccgtt ccccatcgga gagctcctgg cgaaggtgca cagggtcctg
    12481 ggggacgcct gagccgtcag gggcggacgg cgatcgcctc gcggagggtg gccgccgcct
    12541 gctccggcgc catcgggttg atgctcatcc ccgatccgag ctcgaacccc gcgggggtgg
    12601 tcgtgcgggg gaagagctgg aggtgccaga ggaaaaacgg cttccccttc tcgtgggcgg
    12661 gggccgactg cacgatgagg ttgtagtcgg ggtcgcccgc gccctcccgc agggcccgca
    12721 gcaccctgct cagcgcctgg gccaggccct cgagctcgtc gtccggcaca tccccgaacg
    12781 aggggcggtg gcgcctgggt aggatccagg tctcgtaggg gttccgagag gcgaacggcg
    12841 ccagggccac gaaggtctcg ttctcgaaca cgacccggtc gcgggcccca agctcggcct
    12901 cgcggaggtc gtcgtacacg cagtgcccgg tgtcgtcgaa gtaccgggtg gccacggagt
    12961 agcgccgcag ggcgtcgggg gcgaacacgg gggtccccag gatctgcgag tgggggtgcg
    13021 ggagcgaggt gccggcgcgg gccccgaagt tgcggaacac gatcacggac ttcacccatg
    13081 gcctctctcg cagcgcggtg tagcggtccc gccagacccc cagcgctcgg gccaggcgct
    13141 cggtctccat gtcgtcgagg cgctcgtcgt gccttggcga ctccacgatg acctcgtgct
    13201 ggccgacccc cggcatctct cggaagagag cgttcccctg gcgctccgtg ctcccgttcc
    13261 cctggagcgc cgggtagagg ttggcgaaga cgcgctggtc ccacgatccg tcccgctcgg
    13321 tgcgagcgat ctcggggggc gtgcgctcct cgttgccggg gcagaacggg cagtcggcgt
    13381 cgaaggggag tcccgccggg cgctccacgc tcgccgcggc ccgaggcctc gccgcgcggt
    13441 ggggggcgat gatcacccac tctgcggtcg tcggatcctg tctcagcgtc gacacgatcg
    13501 ccctaccgta ccagcctctg cgcaggcatc tcgctagatg gtatcgggag ccagcggcta
    13561 cgcttgatcc cgtggtgatc gacgtctcgg agcgggactt cgaggagcgg gtgctgaagg
    13621 cctcgcgcga ggtcccggtc atcgtggact tctgggcggg gtggtgccgt ccgtgcctgg
    13681 tcctgggacc gatcctggag gggctcgccg aggagcacga agggcgcttc gtgctggcca
    13741 agctcgacgt ggacgccaac caggcgctgg ccgcgcggta tggggtccag gggatccccg
    13801 ccgtgaaggc cttcagggac ggcgaggtcg tcagcgagtt cgtcggggct cagcccgagg
    13861 acgtggtgcg cctgtggcta cgcgaggtgc ttccctccga ggcggagccg atcgtcgccg
    13921 aggccgtcgg gctcgaggag gagggggatc tggagcgggc cgagggcgga taccgtcgag
    13981 ccctggagat cgatcccgat cacgaggagg ccacggtggg cctggcccgg gtgctcctgg
    14041 ctcgtgggga ggccgcagag gcgggtcgcc tcgcatcgcg cctgcccggg agcgtcgagg
    14101 cgcgggccat cgcggcccgg gccgccttgg tcgagcggat ccccggggac ggtgatctcg
    14161 acgacctccg ggtccgagcc gagagcgacc cggaggccgc gctcgcggtg gccgcggccg
    14221 acgccgagca gggccagacc tcggaggcgc tggatcgcct gctggggttg gtcggaggcg
    14281 agacgcgcga ccgggcccgg gacctcatgg tcacgatctt cgagtccctc ggcgacgacc
    14341 acccactcgt ccgggagtac cggccgaagc tcgccagcgc gctgtactga tggccgaggt
    14401 caccgtcgag gagttccagc ggatcgacgt ccgggcgggg accgtcgttc ggtgcgagcg
    14461 gttccccgag gcccgcagcc ccgcctacaa gctgtggatc gacctgggcc cccagatcgg
    14521 ggagcgtcga tcgagcgcca ggatcaccga ccactacgag ccggaggagc tcgtgggcag
    14581 gcaggtcgct gccgtggtga acttcccacc gcggcggatc ggccctttca gctcggaggt
    14641 gctcgtgctc ggcttctacg acgaggacgg aaccgtcgtg ctcgtcgggc ccgaccgctc
    14701 ggtgccgaac ggggccaggc tggggtgagc gtggagtcct cgcgattctc cgtggacacg
    14761 accggacggc tcgtcaccga cctcacggag cgggtccagg acttcgtccg ggatcggggc
    14821 ggagaagggc tgctgaacgt cttccttcca cacgccacgt gcggtctggc tctgatggag
    14881 acgggctcgg gctccgagcg cgacctccag gacgcgctcg acggcctcct ccctcccgag
    14941 gaccggtact cgcaccgtca cggctcgacg gggcacggcc gtgaccacct cctcccgctg
    15001 ttcatctcgc cgtccctgac cctgcccgtg gagggaggcg aggtcgtgct gggcacctgg
    15061 cagtcgatcg tgctcgtgga cacgaaccgc gagaacaacc ggcgggacgt ggtgctctcg
    15121 ctcctgcgcg ggtgaccctc ccggcgctag catccgcccg tgacggagcg cgacgggact
    15181 cagggcctcc tcgatcgcgg ccgagccttc ttcacgtggg ccagccgtcc catgcggcgg
    15241 atgtggacct cggacgatcc gctggaggac tacgcgctcg tccatatggc gagcgtcgcc
    15301 ggcgacaccc tgctggcgat cgcgctggcc gactcgatct tcttcgcgct gccggtgggg
    15361 gaggcccgca cccaggtcgc gctctacctg gggctgacca tggcccctct ggcagtggcg
    15421 ggccccctcc tggtcccgct gctggaccgg agccccttcc ggcgggcgat ctcgttcggg
    15481 gccagcgtgg ggagggccgt ggcagtgctg ctggccgctc gatggctgga ttcgctcctc
    15541 ctgttccccg ccgtcttcac cgtcctggtg ctgacgagga tccatctcat cgcgaagaac
    15601 ggcctgacgg ttgcctacgc tcccaccggc gcggcgctga tgagggcgaa cgccttcctg
    15661 gcccggacgg cgatcgccgg cgcgctgctg gctgcggtcc ccggcgtcgt gatgctgcag
    15721 ctcgggagga cccggggcgt gctcgtcctg gccgcgctgg tgtacgcggc ggccgcgctg
    15781 ctcaacctgc gcctggcgcc cgtggagcgg cacccgcctc gggccgggga ggtcccgcgc
    15841 cgcgggagga tccctgcgct cgcggaggcc gccctcggcg cgggcggcct ccgcggagcg
    15901 cagggattcc tcatggcgct cctggccttc gcgctccggg acgcgggagc cccgacctac
    15961 tggttcggcg tcctcctcgt caccggcacc gcgggcgcgg ccatcgcgga cttcctggtg
    16021 ccgcgtcttc ccacgacggt ccgcgaggag gtgatcgtgg tgggctcgct cctggcagcg
    16081 ggcctggggg cgttcctcgc gtcgcaggcc tacgggctcc cttccctcgc gctgttcgtc
    16141 ggggtcgcgg ggatgtccac ggaggccggc cggctcgcgt tccagagcct gatgcaggcg
    16201 aacgcgccgg gaggcgcgca gggtcgggtc ttcgtccggt acgaggtggc gttccagctg
    16261 gcatgggtga tgggagcgtt catcccggcg atgcttccga tgtcgttccg cggcgggttc
    16321 atcgcgctgg cgcccttcta cctcgcgctg ggggtggtct acgtggtgcg cctggtgcgg
    16381 gcccgccggc tccgggcggg ctgagggacg tcggggggac atccgaggca tggtctcggc
    16441 cggtcctggg ccgtaccctc gagccgtggg ccgcacgctg atctgcctcg tcctgctcgc
    16501 ctccggctgt acggggaccg agcctccgga ggccgggccg acgggcacgc ctcccgccga
    16561 gcagatccag cgcgagtccc acctcgtggc cgggcacgac cggcccgagc tcccctgctt
    16621 ggaggccggc ccggacgacc ccccgcctcg gctcgagatg ggcgacacgt tcttcgctcc
    16681 cagctgcgtc gtcctgcgag gccgccacga ccttcggctg gccaacggtg gcgcgctgga
    16741 gcactccttc ggggtccgag acgccggggt atcggtggac ctcgagccgg gcgcgtccgg
    16801 cgaggtgtcc ctcgaggatc tgcggggatc cggctcgacg gagttcttct gccgcttcca
    16861 cgcctcggcc gggatgacgg gcacgatcac ggtggcctga ctcccgtcgt ctcctgtcgg
    16921 agggggcgcg gggccgctac acttcccgcc gtgagcgacg agggtccccg catcgaggtc
    16981 acccagaacg ggaactaccg ggtccgcggc cgcgtgccga tctacccggg gcgtatcctg
    17041 cgctccgagg atggcagctc gatcgattgg gagctggacg agccctaccc cctccgaagg
    17101 accgtgacgc tgtgccggtg tggccgctcg gggaacaaac ccttctgcga tgacacccac
    17161 cagacgatcg agtgggacgg cgaggagacg gccgaccggg gaccgacgag cgctcggcgc
    17221 aaggcggtgc ccgggcaggg ggtgacgctc accgacgatg tctcgctgtg cacctctgcg
    17281 gcgttctgtc atgaccgcgt gaccgacgtc tggcggctta ccttccaccg ctccgacgag
    17341 ccggaggtcc gccggcgtat cgaggggatc gtggcgaggt gcccttcggg tcggctcgtc
    17401 ctcgagaccc acgacggagc cgaggtcgag cccgagtacg agccctcgat cgtcgtacag
    17461 gagggcgggc cctactgggt gcggggccgg gttccgatca tggccgtgga cgggcgcacg
    17521 tgggaggtgc gcaatcggct ggcgctctgc cggtgcggag cctcaggcaa caagcccttc
    17581 tgcgacagca cccatctcga gatcgggttc gcgtgacccc cccttgtcgt agccggggcg
    17641 cgaccataga ctgagggaac tcgatcgacg ttcgctcgat cacccaacca gtcggcgaat
    17701 cggaacggag gtgagcacta cctatggccg gagtcacggg atcgggcggc gccctcgcgc
    17761 gtcgccgaac catctcggcg gtctccctgg ccctcgtcac catgctgatg gtcgcgctcc
    17821 cggcggtccc cgcatcggcc gcgaaggcac gcgtcaaagc caaggtcggc gcgaacaagt
    17881 gggtgaaggt ccacacctac atcggtaagg gcgacgtggt catctggaag aacccccgac
    17941 cgaagatggt gagcaagcgg agggtccacg acgtcacctc gatccagccc aggcgctgga
    18001 agaagcggct ggggttcaag ctcagcaagg tgctccagcc ggggcagcgg gtgaagagga
    18061 agttccgcag gacgggcacg taccgatacc gctgcgcccg acactcggcg atggtcgacg
    18121 ggaagtgtca ggggatgtgc ggcttcatcc acgtgctctg agcggcctcc ggccgcgcgc
    18181 atgacgggga gggacggtct gaccgcccct ccccatctgt gctcacctct tcgtcgcctc
    18241 gcccgcgttc cccgagaccg gtgccgacag gtatgcgacc agcgaactcc gcgagcgcgc
    18301 acgcccggcc cttgcttcgc atggtggtca tcgcagcgga ggctctctca acgggtcgaa
    18361 cccagacacg atctgccgtc gaattcacag gagactccca gaaggaggag gcatgccgcc
    18421 cggtatagtc tgcgactacc caggatgcag cgaagttggt caactcgtct tacgcgagga
    18481 ggcatgatgg cgacggagaa gcgcgcggac gtgacgtggc ggggggatct cgccacggga
    18541 cggggaacga tcgagcgggt cggcagcggg gtcatcgagg ggttgggtgt ctcgtgggcc
    18601 gctcgtaccg gcgagagcga cgacctcacg agccccgagg agctgatcgc cgcggcgcac
    18661 gcctcgtgct tctcgatggc gctctcgaag atcctggccg atcagggcca cgcccccgac
    18721 cgactgctcg tcacggccac ggtgagcttc gttccgggca ccggcatcac gaggtctgcg
    18781 ctcgacgtca ccggtgtggt gcccgggatg gaggaagcgg agttcgggaa tgcggccgag
    18841 gcggccaagg agaactgccc cgtctcgcaa gcgctcaagg gaaacgtcga gctcacggtg
    18901 gcgtcccgac tctcctgaga ctcccggccg acgatccgtt tcgggatggg tctcctgggt
    18961 agttcgagcg cgaccaggaa ggagggtgag tctcatacgt acgcgactga tctcgctcgt
    19021 gaccgtggtg atcgtggccc tgggcgttcc gagccttgcg tcggccgctc cgggcgggga
    19081 catccgcgag gcctgcggcg cttcgttcgg acagctcgtg tcggccggca ggtcctcagg
    19141 cacggcgttc cacacgaact acgcgggggg cgccatggcg ttcagcgatc cggccgtgct
    19201 ggccgctcac ggctgcacgc tctgacggac gaggccgagc ggctccctcc cacgggaggg
    19261 agccgctcgg cctacccgat ctttcctaga cctatccggg agaagaactc gctcgccgag
    19321 tctcccccaa cgccgctctg ggtgaacagc gacaggaagt ccgtgccgtc ggtctcgagg
    19381 cccacgtagt caccgaggaa gaacccgttc gcgaacggcg cctcggtgat gtcgaaggag
    19441 gacgtcgtga cctgcgtttc ctcatcccag ctggcctcgt tcgcgcagtc ctccgacgcg
    19501 ggatggcagt gcgcgacgaa catgtccgtc gtcccgaccc cgtcgtcgtc cacgttgttc
    19561 cggaagtcgt agtagctgac ggcgaccgtg ccgtcgtcga gcacgcggac catgggcgtg
    19621 aacgcctgcc ggttccccag tcggatgttc tcgggcgtct ggttgacctt gatcggcgtc
    19681 gtccaggtga agcctccgtc ggtcgacatc gtgaaggcga cctcatcgat caggtccagg
    19741 acggtcgtgg cccccggtaa ggtgaaccgc gcgtccatcc agaccacgta caggttgccg
    19801 ttgttcggat cgaccgcgac ctccgggatc acctcgccgc tccggacggc gttggcgccg
    19861 tccagcgtct ccaggccgtc cgcgtcgggc tgatacacgc tcacgaacgg gaagacggga
    19921 ttgaacagcg tccgggggag catgtccccg aacctgatcg gggggccctt cggcagccac
    19981 gtccgcccag cgtccggcga gaccttgagc gccaagttga agtcgaactg cgcgtcccgg
    20041 tcgtcgttcc ggaagttcag gatctcgttg aagacgttga ccagcgtccc gtcgggccgc
    20101 accacgatct ggttcccgat cgtctgattg accccgcccg ggttgtagag gcgcctcggc
    20161 gtcgaccagg tcatgccgcc gtccgtcgtg cgcgagatca gcgagccacc cttgaagccg
    20221 agcccgcccc cgaacgtgcc tcggtcgggg ttgatcgttc cgcccggagg gacgtcgagg
    20281 aagtcccaca cggcgtaggc gaacccg
//
LOCUS       DASUTZ010000041         7087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_918097, whole genome shotgun sequence.
ACCESSION   DASUTZ010000041 DASUTZ010000000
VERSION     DASUTZ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7087
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918097"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VF097_01915"
     CDS             <1..198
                     /locus_tag="VF097_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6261583.1"
                     /translation="AVDVGAEALEDLEERAKEAKTSVVAQLNPRTSASEGEEIELYVD
                     TSRMHFFDKDSGLGIYDGSPS"
     gene            276..1571
                     /locus_tag="VF097_01920"
     CDS             276..1571
                     /locus_tag="VF097_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004930734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6261584.1"
                     /translation="MLALLSVLALAAAACGDGDGDGDGEAEPDAPQDLSGETVEVAAV
                     WTGTEQESFQQVLSAFSEQTGAETQFTSTGDDVAAVLGTRIEGGDPPDVAFLPQPGLM
                     ADLAEQDALIPIEDVAGDEVDANYSQDWRDLGSIDGTLYGVWFKAANKSTVWYNTNVF
                     EQAGVSAPEDWDGMMSAAQTVSDFGVTPWSIGGADGWTLTDWFENVYIRTAGPEMYDQ
                     LTNHEIPWTDQSVKDALTRLAEIFENQDMLAGNPLQTDFPTSVANVFANPDSPDAAMV
                     YEGDFVAGVISGETGSTVGEDADFFNFPSIDGSPLAVVGGGDVAVLLTDSEAGRALME
                     FLASPEAGEVWAGLGGFTSPNQNVDLSVYPDDITRRSAEALATAETFRFDMSDLQPAE
                     FGGTVGQGLFSLFQDFLADPSAIDQVTEQMEQEASQAFG"
     gene            1582..2985
                     /locus_tag="VF097_01925"
     CDS             1582..2985
                     /locus_tag="VF097_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014142755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX6261585.1"
                     /translation="MAGTDERTPDQVAATAGGPVAVEEKPPRPPGRGRRDRVKTWVVA
                     GGFLAPALIILGALVLYPIIFSVVRSLYDKPGDAFVGLANYTDMLSSGRTLTAIRNNV
                     IWVVVAPTVITALGLVFAVLTERVRWGTAFKVAIFMPMAISFLSAGVTWRVMYQQEPE
                     RGLINAAIGGVTNVFRSPGSFPGARPSQEDLLQPAGPGGEGQGRALATTRTFATGETV
                     NLGLVAVNPELVPEAAEPAQPPQVPSGGIGGVVWLDFTPGGEGERGQIDSQEVGLPGV
                     TVEAVQNGEVVGSATTESDGSYVIEDLGSGEFTMQLARSNFQQAFGGVPWLGPALVTP
                     AIIAAYIWMWAGFAMVVIGAGLAAIPRDVLEAARVDGANEWQVFRRVTVPLLAPVLAV
                     VLVTLIINVLKIFDLVLVIAPGNVQDDANVIALEMWRTAFGAQNDRGLGSALAVFLFV
                     LVVPAMLFNLRRFKSEQ"
     gene            3035..3874
                     /locus_tag="VF097_01930"
     CDS             3035..3874
                     /locus_tag="VF097_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019359660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX6261586.1"
                     /translation="MGRIMHAVSRGPVAIVLVIVGLLWLVPTFGLLITSLRSNAANLE
                     SGWWNVVTAPSQLSLESYRGILDDPTMLSGLWNTVLITVPTSLLLVMIAAGAAYAFAW
                     ARFPGRDALFLVVVGLLVVPIQIGLIPISSLFGRLNLFGTIPGVVLYHIAFGLPFAIF
                     LLRNFFIGIPKELLEAARMDGASEWRVFTRVVLPLGIPAIASLLIFQFLWTWNDLLVA
                     LVFAGTDNAPLTVIIREQTRQFGSNIGIIAPAAFLQMIVPLIVFFAFQRYFVQGLLAG
                     STK"
     gene            3871..5370
                     /locus_tag="VF097_01935"
     CDS             3871..5370
                     /locus_tag="VF097_01935"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX6261587.1"
                     /translation="MTRRLLIATIVVVLAGGGLIAARSGGLRIEVPAGADAASVLERA
                     PQGSTLVLLAGRHAPFAIDRRVTVEAEGGAVVSGPLVVRADGARLQGLTIQGGETGVL
                     VREADDVVLRGVLVEGAADHGIEVVNASAWIEGCVIRRLTGGMAQGIEIRNANGRPRS
                     VISGCAVRGGMEGIVSHVSRLEAVDNVVTGTTVRGISITEMSEGLVEGNVVRDALGSG
                     IYCGDMSHCEVRRNTIRDIESDGSGITSRSGYGVVALYYATMRVHQNELVDVPNRVGT
                     LVGSVTVDRFPLSIWAQGWQGLLPGIIVVVALALAALALARWAVTPWVRRWRRRARPI
                     DPGKALPILVGGFAVQSFHMLEHVVQVYQVHVIDAEIRSGLAGQKIDTEWVHFVYNLA
                     VLAFIVWVWRLVRPGAPLAGGVGKSAGFLLAAFAVQGYHMAEHTAKLVQHLSLGIDPA
                     PGIIGGAAGLVWFHFGINLTVYVGMAIPVVSAIRTAYGGGRDLVVEPGR"
     gene            5367..6443
                     /locus_tag="VF097_01940"
     CDS             5367..6443
                     /locus_tag="VF097_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX6261588.1"
                     /translation="MTPDSLGILRSGRGPSGAILASPSFPVYRYSWLRDGAFIAEAFA
                     ASREREAAKAFHRWAAATVERFAHKVEALERRIPDPLTDADVLHPRYTEEGEEVDDPL
                     WSNFQLDGHGFWLATLARHVEAGIAEPAEVLGAVDLTTRYLRLLWDRPCADCWEEHDG
                     LIHPTTLCAVAEGLLGAARLLDDPEPAETARAALARVRERGLTSDGSLAKHEGVEDVD
                     ASALLVLGSLGPFEPDDAIVGATLRRIEADLVADGGGVHRYLDDGYYGGGLWVVLSGS
                     LARVHALAGNSDRAREVLAWVEATADQDGALPEQVSTHLRKPDLLPVWEARWGPVAKP
                     LLWSHAMQVLARSALDQPEPPISS"
     gene            complement(6416..6664)
                     /locus_tag="VF097_01945"
     CDS             complement(6416..6664)
                     /locus_tag="VF097_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261589.1"
                     /translation="MAADEFDERFHEAVTALDEIAGEIKPPEAWDGFDPVSLEEFWQS
                     WPTVRAWGEWLWQLVEAERGEKAAPANDSPYEEIGGSG"
     gene            6730..>7087
                     /locus_tag="VF097_01950"
     CDS             6730..>7087
                     /locus_tag="VF097_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX6261590.1"
                     /translation="MPNRLQGATSPYLLQHADNPVDWYEWGPEALERARAEDRPILLS
                     VGYAACHWCHVMERESFEDEDTAALMNEYFVNVKVDREERPDVDAIYMDAVQAMTGQG
                     GWPMTVFLTPEGEPFWA"
BASE COUNT         1004 a         2387 c         2602 g         1094 t
ORIGIN      
        1 gcggtggacg tcggggccga ggcgctcgag gacctggagg agcgagccaa ggaggccaag
       61 acctcggtcg tggcccagct caaccccagg acctcggcgt cggagggaga ggagatcgag
      121 ctgtacgtgg acacgtcccg gatgcacttc ttcgacaagg attcggggct ggggatctac
      181 gacggctcgc cctcgtgagg tcggccggcg agaagagagg ggaaccgtac ccaagggggg
      241 tgcacacggg aatgagacga gccaaatggt acaggatgct tgcgctgctc tcggtcctcg
      301 cgcttgctgc ggcggcgtgt ggagacgggg acggcgacgg ggacggcgag gcggagcccg
      361 acgccccgca ggacctgtcc ggtgagacgg tcgaggtggc cgccgtctgg acggggaccg
      421 agcaggagag cttccagcag gtgctgtcgg cgttctccga gcagaccggc gcggagacgc
      481 agttcacctc gacgggcgat gacgtcgcgg cggtgctcgg gaccaggatc gagggagggg
      541 acccgcccga cgtggcgttc ctgccccagc cgggcctgat ggcggacctc gccgagcagg
      601 acgcgctcat cccgatcgag gacgtcgccg gcgacgaggt ggacgccaac tactcgcagg
      661 actggcgcga cctcgggagc atcgacggga ccctctatgg ggtctggttc aaggccgcga
      721 acaagtcgac cgtctggtac aacacgaacg tcttcgagca ggcgggcgtc tcggcccccg
      781 aggactggga cgggatgatg tcggcggccc agacggtctc ggacttcggc gtgacgccgt
      841 ggtcgatcgg gggggccgac ggctggacgc tgaccgactg gttcgagaac gtctacatcc
      901 ggacggcggg cccggagatg tacgaccagc tcacgaacca cgagatcccg tggacggatc
      961 agtcggtgaa ggacgcgctg acgaggctgg ccgagatctt cgagaaccag gacatgctgg
     1021 ccgggaaccc gctccagacc gacttcccga cctcggtggc gaacgtgttc gcgaacccgg
     1081 actcgccgga cgcggcgatg gtctacgagg gcgacttcgt ggccggcgtg atctcaggcg
     1141 agacgggctc cactgtgggt gaggacgcgg acttcttcaa cttcccctcg atcgacggtt
     1201 cgcctctcgc ggtcgtcggc ggtggcgacg tcgcggtgct cctcaccgac tcggaggcgg
     1261 gtcgggcgct catggagttc ctggccagcc cggaggccgg cgaggtgtgg gccgggctcg
     1321 gcgggttcac gtcgcccaac cagaacgtcg atctgagcgt gtaccccgat gacatcacca
     1381 ggcggtcggc ggaggccctg gcgacggccg agaccttccg tttcgacatg tcggacctcc
     1441 agcccgcgga gttcggcggc acggtcgggc agggattgtt cagcctcttc caggacttcc
     1501 tggccgaccc gtcggcgatc gatcaggtga ccgaacagat ggagcaggaa gcgtcacagg
     1561 ccttcggcta ggagggatcg gttggccggg accgacgagc gcacgcccga ccaggtagca
     1621 gccacggccg gcgggccggt ggcggtggag gagaagccgc cccggccccc cggtcgaggg
     1681 aggcgggacc gggtcaagac ctgggtggtc gcgggcgggt tcctcgcgcc cgccctgatc
     1741 atcctgggcg cgctcgtgct ctacccgatc atcttcagcg tcgttcgcag cctgtacgac
     1801 aagcccggcg acgcgttcgt ggggctggcg aactacacgg acatgctctc gtcgggccgc
     1861 acgctcaccg cgatccgcaa caacgtgatc tgggtcgtgg tagccccgac cgtgatcacc
     1921 gcgctcgggc tcgtcttcgc cgttctcacg gagcgtgtgc gctgggggac ggccttcaag
     1981 gtggcgatct tcatgccgat ggcgatctcg ttcctgtccg ccggcgtgac ctggcgggtg
     2041 atgtaccagc aggagcccga gcgcgggctg atcaacgcgg cgatcggggg cgtgaccaac
     2101 gtcttccggt cgcccgggag cttcccgggg gcccgaccat cccaggagga cctgctccag
     2161 ccggccggcc cgggaggtga gggccagggg agggccctcg ccaccacccg gacgttcgcc
     2221 acaggggaga ccgtgaacct cggcctcgtc gcggtcaacc ccgagctcgt ccccgaggcc
     2281 gcggagcccg cgcagccgcc gcaggtgccc tcgggcggga tcggaggcgt cgtgtggctc
     2341 gacttcaccc ctggaggcga gggcgagcgg gggcagatcg actcacagga ggtgggcctg
     2401 cccggcgtga cggtcgaggc cgtccagaac ggggaggtcg tcggcagcgc cacgacggag
     2461 agcgacggat cctacgtcat cgaggatctg ggctcgggcg agttcacgat gcagctcgca
     2521 cgctccaact tccagcaggc cttcggtggg gtcccgtggc tggggcccgc gctcgtcacc
     2581 cccgccatca tcgcggcgta catctggatg tgggcggggt tcgccatggt cgtgatcggc
     2641 gccggcctgg cggcgatccc ccgcgacgtg ctggaggccg cgcgcgtgga cggcgccaac
     2701 gagtggcagg tgttccggcg ggtgaccgtg cccctgctgg cccccgtgct ggcggtcgtc
     2761 ctcgtcacgc tgatcatcaa cgtcctgaag atcttcgacc tcgtcctggt catcgcgccc
     2821 ggcaacgtcc aggacgacgc caatgtgatc gcgctggaga tgtggcggac cgcgttcggc
     2881 gcccagaacg accgtggcct gggaagcgcg ctggcggtgt tcctgttcgt gctggtcgtg
     2941 cccgcgatgc tcttcaacct cagacggttc aagtcggagc agtgagatgg cgacggacgc
     3001 gacggtagcg gcgccctccc ggccctcgac cccgatggga aggatcatgc acgcggtctc
     3061 gcgcggaccg gtggcgatcg tgctcgtgat cgtggggctc ctgtggctgg tgcccacgtt
     3121 cgggctgctg atcacctcgc tccgctccaa cgcggcgaac ctggagagcg gctggtggaa
     3181 cgtcgtcaca gcgccgagcc agctctcgct ggagtcctac cgcgggatcc tcgacgaccc
     3241 caccatgctc agcggcctgt ggaacacggt cctgatcacc gtccccacgt cgctgctgct
     3301 cgtgatgatc gcggcgggcg ccgcgtacgc gttcgcttgg gcccggttcc ccggccgcga
     3361 cgccctcttc ctcgtggtcg tcgggctgct ggtggtcccg atccagatcg ggctgatccc
     3421 catctccagc ctcttcgggc ggctgaacct cttcgggacg atccccgggg tggtcctcta
     3481 ccacatcgcc ttcgggctgc ccttcgccat cttcctgcta cgcaacttct tcatcgggat
     3541 ccccaaggag ctgctcgagg cggcgcggat ggacggggcc agcgagtggc gggtcttcac
     3601 gcgcgtcgtg ctgcccctcg gtatccctgc gatcgcctcc ctgctgatct tccagttcct
     3661 gtggacgtgg aacgacctgc tggtcgccct cgtgttcgcg gggaccgaca acgcccccct
     3721 gaccgtgatc atcagggagc agacgcggca gttcggatcg aacatcggga tcatcgcccc
     3781 cgccgcgttc ctccagatga tcgtgccgct gatcgtcttc ttcgcgttcc agcgatactt
     3841 cgtccagggc ctgctggccg ggtcgaccaa gtgaccaggc gcctgctgat cgccacgatc
     3901 gtcgtcgtcc tggccggcgg cggcctgatc gcggcccgct ccggggggct gcggatcgag
     3961 gtgccggccg gcgccgacgc cgcctcggtg ctcgagcgcg ccccgcaggg ctcgacgctc
     4021 gtcctgctcg cgggacgaca cgccccgttc gcgatcgacc ggcgagtcac cgtggaggcg
     4081 gaggggggcg ccgtggtgtc ggggccgctt gtcgtgcgtg cggacggcgc gcgcctccag
     4141 ggcctcacga tccagggcgg cgagaccggg gtcctcgtgc gcgaggccga tgacgtcgtg
     4201 ctgagaggcg tcctcgtgga gggcgcagcc gaccacggga tcgaggtggt gaacgcgtca
     4261 gcctggatcg agggttgcgt gatccggcgg ctcacggggg ggatggccca gggcatcgag
     4321 atccggaacg cgaacgggcg gccccggagc gtgatctcgg ggtgcgccgt tcgcgggggc
     4381 atggagggca tcgtctccca cgtgagcagg ctggaggccg tggacaacgt cgtgacgggg
     4441 acgaccgtcc ggggcatctc gatcaccgag atgagcgagg gcctcgtcga ggggaacgtg
     4501 gtccgcgacg ccctgggctc ggggatctac tgcggcgaca tgtcgcactg cgaggtgcgc
     4561 cggaacacga tccgcgacat cgagagcgac gggtccggga tcacgagccg ctccggctac
     4621 ggggtcgtgg cgctgtacta cgccacgatg agggtccacc agaacgagct ggtcgacgtc
     4681 ccgaacaggg tggggacgct agtgggatcg gtgaccgtgg accgcttccc gctctcgatc
     4741 tgggcgcagg gatggcaggg gctgctgccc ggcatcatcg tggtcgtggc gttggcgctc
     4801 gctgcgctcg cgctggcccg ctgggcggtc acgccgtggg tgcgccggtg gcggaggcgg
     4861 gccaggccga tcgatccggg gaaggcgctg cccatcctcg tcgggggctt cgcggtgcag
     4921 agcttccaca tgctcgagca cgtcgtgcag gtctaccagg tgcacgtgat cgacgccgag
     4981 atccggagcg ggctcgcggg tcagaagatc gacaccgagt gggtgcactt cgtctacaac
     5041 ctggcagtgc tcgcattcat cgtctgggtg tggaggctcg tgcggcccgg ggccccgctg
     5101 gcggggggcg tggggaagag cgccggcttc ctgctggcgg cgttcgcggt gcagggctac
     5161 cacatggcgg agcacacggc gaagctggtc cagcacctct cgctcgggat cgaccccgcg
     5221 ccgggcatca tcgggggcgc cgcgggtctg gtctggttcc acttcgggat caacctcacg
     5281 gtgtacgtgg gcatggccat cccagtggtc tcggcgatcc gcacagcgta cggcggcggg
     5341 cgcgacctcg tggtcgagcc cggccggtga cgcccgacag cctcgggatc ctgcgatcgg
     5401 ggcgcgggcc gtccggcgcg atcctcgcct cgccctcctt tcccgtctac cggtactcgt
     5461 ggctgaggga cggggccttc atcgcggagg ccttcgccgc ctcgcgcgag cgcgaggcgg
     5521 cgaaggcctt ccaccgttgg gccgcggcga cggtcgagcg attcgcgcac aaggtcgagg
     5581 cgctggagcg ccgcatcccc gatcccctca ccgacgcgga cgtcctccac ccccgctaca
     5641 cggaggaggg ggaggaggtg gacgaccccc tctggtcgaa cttccagctc gacgggcacg
     5701 gcttctggct cgcgacgctc gcccggcacg tcgaggcggg gatcgcggag ccggccgagg
     5761 tgctgggcgc cgttgacctc acgacacgct acctccgcct cctgtgggac cgcccctgcg
     5821 ccgactgctg ggaggagcac gacggcctga tccaccccac caccctgtgc gcggtcgcgg
     5881 agggcctcct cggagcggcg cgactcctgg acgacccgga gcccgccgag acggcccgcg
     5941 cagccctggc ccgggttcgg gagcggggac tcacctccga cggctctctc gccaagcacg
     6001 agggtgtgga ggacgtggac gccagcgcgc tcctcgtgct cgggtcgctc gggcccttcg
     6061 agcccgacga cgcgatcgtc ggggcgacgc ttcgccggat cgaggccgac ctcgtcgcgg
     6121 acggcggagg cgttcatcgg tacctcgatg acggctacta cgggggcggg ctgtgggtgg
     6181 tgctgtcggg atcgctggcg cgcgtccacg cgctggcggg gaacagcgac cgagcccgcg
     6241 aggtcctggc ctgggtggag gccacagccg accaggacgg cgcgctgccc gagcaggtct
     6301 ccacccacct gcggaagccc gatctcctac cggtgtggga ggcgagatgg gggccggtgg
     6361 ccaagccact cctctggtcc cacgcgatgc aggtcctcgc gcggagcgcg ctggatcagc
     6421 ccgagccgcc gatctcctcg tagggggagt cgttggccgg cgccgccttc tcgccccgct
     6481 ccgcttcgac gagctgccac agccactcgc cccaggcccg gacggtgggc cacgactgcc
     6541 agaactcctc cagggacacc ggatcgaagc cgtcccaggc ctcgggcggc ttgatctcgc
     6601 ccgcgatctc gtcgagcgcc gtaacggcct cgtggaacct ctcgtcgaac tcgtccgcgg
     6661 ccatgctgcc tccctcgggg gcagtctagc ggggcgtcgc cggatgctcc accgggtagc
     6721 atcgcctcga tgcccaaccg actccaaggc gccaccagcc cctacctgct ccagcacgcc
     6781 gacaacccgg tggactggta cgagtggggc cccgaggccc tcgagcgcgc tcgcgccgag
     6841 gaccggccca tcctgctctc cgtgggctac gccgcctgcc actggtgcca cgtgatggag
     6901 cgggagtcgt tcgaggacga ggacacggcc gcgctcatga acgagtactt cgtcaacgtg
     6961 aaggtggacc gcgaggagcg gcccgacgtg gacgcgatct acatggacgc cgtgcaggcc
     7021 atgaccgggc agggcgggtg gccgatgacg gtcttcctca cgcccgaggg cgagccgttc
     7081 tgggccg
//
LOCUS       DASUTZ010000042         4738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1824723, whole genome shotgun sequence.
ACCESSION   DASUTZ010000042 DASUTZ010000000
VERSION     DASUTZ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1824723"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /gene="fdxA"
                     /locus_tag="VF097_01955"
     CDS             complement(<1..207)
                     /gene="fdxA"
                     /locus_tag="VF097_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX6261591.1"
                     /translation="MAYVITEPCIDVKDRACVDECPVDCIYEGGRMLYIQPDECVDCD
                     ACEPVCPVTAIFAEDDVPEEWKNFT"
     gene            complement(284..1264)
                     /locus_tag="VF097_01960"
     CDS             complement(284..1264)
                     /locus_tag="VF097_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6261592.1"
                     /translation="MGDGFAIALNGYGLRREDGHREVLAWHDVVQIARTAEEGGYRAI
                     FVPEIGAREAFSTLVGLAAETSRVRLVSGVAPIGSRDPRRMAMEAATLQDLSNGRGVL
                     GLGSREPIERTREEIAAIRALLAGEEPKVEGDGAAALPGLDLFPGRMPIYLAALGPRM
                     TELAGEMADGVILNWCTPERVSQAHEQLARGAARAHRDVSTATVCVYVRAAVGMSEEL
                     ALEALRAALAEYAGMPTYARQLEAMGLGAECRRAAEATSLGEVPESLVDALCVREGRD
                     EALARLGEYHDAGADVVVVYPVPELDPATSITGTVLGLAPSPALDPTASR"
     gene            1287..1931
                     /locus_tag="VF097_01965"
     CDS             1287..1931
                     /locus_tag="VF097_01965"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6261593.1"
                     /translation="MTTTTFETDDGVTLEGELREPEGEARGTAVICHPHPAHGGSKDH
                     PLLWAIRNELAQRGWVVLAFNFRGVMGSGGEHSGGQEETRDLRAAIDRVRDEAEGPTV
                     VVGWSFGANVAVRHSITDERVAAVALVGLPIGFDLLPVVNLPENEKLRAWETPVLLVA
                     GDDDAICPVPGMLELSGRIPNADVEVIEGGGHFFRRKEREPAKAIADFFDEVLQ"
     gene            complement(1925..2128)
                     /locus_tag="VF097_01970"
     CDS             complement(1925..2128)
                     /locus_tag="VF097_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261594.1"
                     /translation="MALLILIILIIAAVTGTLWQVLEIAAGVAIGIFLATVLLAVAAY
                     YAVRSRVRRFQRDMRRDDRPERY"
     gene            2199..2954
                     /locus_tag="VF097_01975"
     CDS             2199..2954
                     /locus_tag="VF097_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016434513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX6261595.1"
                     /translation="MEVLIGTSGWQYDDWRGVLYPERVAKKDWLAHYSERFPVVEVNN
                     TFYNLPADDTFVKWRRGTAEGFRFVVKASRFITHIRRLRECRDPVQLLWSRAERLGRK
                     LGPVLFQMPPNLRADHERLRAFLEVLPKHMEAAFEFRHRSWESDDTYDLLDRGGYAYV
                     LADRPGARVPDVVTGGWSYLRFHQGTEDGPGYTRRKLRRWADRIAGLDARTVYVFFNN
                     DQTCAAVRDARTLTHLLEDRHDLRVKGPVPEAA"
     gene            2979..3947
                     /locus_tag="VF097_01980"
     CDS             2979..3947
                     /locus_tag="VF097_01980"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6261596.1"
                     /translation="MKVFLTGGTGFVGGEVARQLRGRGDEVVALVRTRSKATSLIELG
                     CSLVEGDLNAVDAIREAVEGCDGVVHSAGMYEVGIPRAERPRMFDANVRGAERVLDAA
                     IDAGVSHILHVSTIGVFGNTRGGVHPGSPTPERRDFLSVYDETKYLAHRLAERHAESG
                     APVTIAAPGAVYGPGDHSEVGNQLDQLRAGKLRFVAFPETGFNAVHVEDAAGGILRVL
                     DRGEVGATYPLGGEITTLGDILGTAARILGRKLPPRIPAALIKASIPFGRLIGPVIGA
                     PPNLREVVRAADGVTYWASDARARDDLGYAPRDLETGLRETLTAAP"
     gene            complement(3931..>4738)
                     /locus_tag="VF097_01985"
     CDS             complement(3931..>4738)
                     /locus_tag="VF097_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6261597.1"
                     /translation="SGSKAEPLVTDPRIKKVSFTGSGPVGWRLKGLDPRKRFTLELGG
                     NAGVIVHSDADLDHAAERIAFGGYYQAGQSCIAVQRVLVQSEVYDDFVERFVKQVQAL
                     KVGDPLDPSTDVGPVIDAGALDRIAEWVDEAVKGGGEILAGGERDDPFYRPTILGQTT
                     PEMKVRCEEIFGPVTTVTSYETWEDALAEVNNSEYGLQAGVFTSSLDRAFEAHRELQV
                     GGVVVNDQSAFRADQMPYGGAKESGYGREGLRYAMEDMTEPRIMVLKVPL"
BASE COUNT          704 a         1732 c         1622 g          680 t
ORIGIN      
        1 ggtgaagttc ttccactctt cgggaacgtc gtcctcggcg aagatggccg tcaccgggca
       61 gaccggctcg cacgcgtcgc agtccacgca ctcgtcgggc tggatgtaga gcatgcggcc
      121 gccctcgtag atgcagtcca cggggcattc gtccacgcac gcccggtcct tgacgtcgat
      181 gcacggctcg gtgatgacgt aggccacgct gtcctccctg gttttcccga ttcccacggt
      241 gtcgaccgtg ggaaccccag tgtacccgcg agcgcgctcg tcctcaacgg gaagccgtgg
      301 ggtccagggc cggcgaagga gcgagcccca ggacggttcc cgtgatcgac gtggcggggt
      361 cgagctcggg gacggggtac acgacgacga cgtccgcgcc ggcgtcgtgg tactcgccga
      421 ggcgggccag ggcctcgtcc cgcccctcgc gcacgcagag cgcatcgacc agcgactcgg
      481 ggacctcgcc cagcgaggtg gcctccgccg cacgacgaca ctccgcgccc aggcccatcg
      541 cctccagctg gcgggcgtag gtgggcatcc cggcgtactc ggcgagcgcg gcccggagcg
      601 cctccagcgc gagctcctcg ctcatgccga ccgcggcccg cacgtagacg cagaccgtgg
      661 ccgtcgagac gtcccggtgc gcccgggcgg cgccacgggc cagctgctcg tgggcctgcg
      721 agacccgctc gggcgtgcac cagttgagga tcaccccgtc ggccatctcg ccggccagct
      781 cggtcatccg cggcccgagc gcggcgaggt agatcggcat gcgaccgggg aagaggtcca
      841 gccccgggag cgcggcggcg ccgtcaccct cgaccttcgg ctcctctccc gccagcaggg
      901 cccggatcgc ggcgatctcc tcacgggtcc tctcgatcgg ctcccgcgac ccgaggccga
      961 ggacccctcg gccgttcgac aggtcctgga gcgtggcggc ctccatcgcc atccgcctcg
     1021 ggtcgcggga gccgatcggc gccaccccgc tcacgagacg gaccctcgac gtctcggccg
     1081 cgagcccgac cagcgtcgag aacgcctcgc gggccccgat ctcggggacg aagatcgccc
     1141 ggtagccgcc ctcctcggcc gtcctcgcga tctgcacgac gtcgtgccac gcgagcacct
     1201 cccggtgccc gtcctccctc cggaggccgt agccgttcag cgcgatcgcg aacccatccc
     1261 ccatcgtccc cgtatcatcc tccatcgtga ccaccaccac cttcgagacc gacgacggcg
     1321 tcaccctcga gggggagctc cgcgagccgg agggagaggc gcgcggcacc gccgtcatct
     1381 gccaccccca ccccgctcac gggggcagca aggaccaccc tctgctctgg gcgatccgga
     1441 acgagctggc ccagcggggc tgggtcgtcc tcgccttcaa cttccgaggc gtgatgggct
     1501 ccgggggcga gcacagcgga ggccaggagg agacgaggga cttgcgggcg gcgatcgacc
     1561 gggttcggga tgaggccgag ggccccaccg tggtcgtcgg ctggtccttc ggagcgaacg
     1621 tggccgtccg tcactcgatc accgacgagc gcgtcgcggc cgtggccctc gtcggcctgc
     1681 caatcgggtt cgacctgctg ccggtggtca acctccctga aaacgagaag ctccgggcgt
     1741 gggagacgcc cgtcctgctc gtggccgggg acgacgacgc gatctgcccc gtgccgggga
     1801 tgctggagct ctccggccgc atccccaacg cggacgtgga ggtgatcgag ggcggaggtc
     1861 acttcttccg caggaaggag cgagagcccg cgaaggcgat cgccgacttc ttcgacgagg
     1921 tgcttcagta gcgctcgggg cgatcgtccc gccgcatgtc gcgctggaag cggcgcacgc
     1981 ggctccgcac cgcgtagtac gcggccaccg cgagcagcac ggtcgccagg aagatcccga
     2041 tggcgacgcc ggccgcgatc tccagcacct gccacagggt gcccgtgacc gccgcgatga
     2101 tcaggatgat gaggatcagc agtgccacac ccccatcctt ccccaagttc cggcgtctcg
     2161 cacgttgccg atgacgagaa acggggcaag gtgagggcat ggaggtcctg atcggcacga
     2221 gcggctggca gtacgacgac tggcgcggcg tgctgtatcc ggagagggtg gccaagaagg
     2281 actggctggc ccactactcc gagcgcttcc ccgtggtcga ggtcaacaac accttctaca
     2341 acctgccggc ggacgacacc ttcgtgaagt ggcgtcgcgg gaccgccgag gggttccgtt
     2401 tcgtggtgaa ggcgagcagg ttcatcaccc acatccgcag gcttcgtgag tgtcgggacc
     2461 cggtgcagct cctctggtcg agggccgagc ggctggggcg gaagctgggc cccgtcctgt
     2521 tccagatgcc tccgaacctg cgtgccgacc acgaacggct ccgcgccttc ctcgaggtcc
     2581 tccccaagca catggaggcg gcgttcgagt tccgccaccg ctcgtgggag agcgacgaca
     2641 cgtacgacct cctcgaccgg ggcgggtacg cgtacgtcct ggccgaccga cccggcgcgc
     2701 gcgtgcccga cgtcgtgacc gggggatggt cctacctccg cttccaccag gggacggagg
     2761 acggccccgg ctacacgagg cggaagcttc gccggtgggc cgaccggatc gccggcctcg
     2821 acgcccgcac cgtctacgtc ttcttcaaca acgaccagac ctgcgcggcg gtgcgagacg
     2881 cccgcacgct gacccatctg ctcgaggatc ggcatgacct ccgcgtgaag ggcccagtgc
     2941 ccgaggcggc ctgacgctgc catcggtagg ctcccagcgt gaaggtcttc ctcaccggcg
     3001 gcacgggatt cgtgggcggc gaggtcgccc ggcagctgcg aggccgcggc gacgaggtcg
     3061 tagcgctcgt ccgcacacgg tcgaaggcca cctcgctcat cgagctcgga tgctccctcg
     3121 tcgagggcga cctcaacgcc gtcgacgcga tccgcgaggc ggtcgagggc tgcgacggcg
     3181 tggtgcactc ggccgggatg tacgaggtgg gcatcccgcg tgcggagcgg ccccgcatgt
     3241 tcgacgcgaa cgtccggggg gcggagcgcg tgctcgacgc cgcgatcgac gccggcgttt
     3301 cccacatcct gcacgtctcc acgatcgggg tgttcgggaa cacgaggggt ggggtgcatc
     3361 cggggagtcc cacgccggag cggcgcgact tcctcagcgt ctacgacgag acgaagtacc
     3421 tcgcccaccg cttggcggag cggcacgccg agagcggcgc gccggtcacg atcgccgccc
     3481 ccggagccgt gtacgggccg ggcgaccact ccgaggtcgg gaaccagctc gatcagctcc
     3541 gcgcagggaa gctccggttc gtggccttcc cagagacggg gttcaacgcg gtccacgtgg
     3601 aggatgccgc cggcgggatc ctgcgcgtcc tggatcgggg cgaggtcggt gccacctatc
     3661 ctctcggcgg cgagatcacg acgctcggcg acatcctggg gacggcggcc cggatcctcg
     3721 gacggaagct gccgcccagg atccccgcgg cgctgatcaa ggcgagcatc cccttcgggc
     3781 gcctgatcgg gcccgtcatt ggcgcgcccc cgaacctccg cgaggtggtc cgggcggccg
     3841 acggggtgac ttactgggcg agcgacgccc gggcccgcga cgacctgggc tacgctcccc
     3901 gggacctgga gaccggcctc cgcgaaaccc tcacagcggc accttgagga ccatgatccg
     3961 gggctcggtc atgtcctcca tcgcgtagcg caggccctcg cggccatagc ccgactcctt
     4021 cgcgccgccg tatggcatct ggtcggcccg gaacgccgac tggtcgttga ccaccacgcc
     4081 cccgacctgc agctcccggt gcgcctcgaa ggcccggtcg agcgacgacg tgaacacgcc
     4141 cgcctggagg ccgtactcag agttgttgac ctcggccagc gcatcctccc aggtctcgta
     4201 cgatgtgacc gtcgtcaccg ggccgaagat ctcctcgcac cgcaccttca tctcaggcgt
     4261 cgtctgcccc aggatcgtcg gccggtagaa cgggtcgtcc cgctcgccgc cggcgaggat
     4321 ctcgcccccg cccttcacag cctcgtccac ccattcggcg atccgatcca gcgcccccgc
     4381 gtcgatcacc ggcccgacgt cggtggaggg atcgagcggg tcccccacct tcagcgcctg
     4441 cacctgcttc acgaatcgct ccacgaagtc gtcgtagacc tcggactgga cgagcacgcg
     4501 ctggacggcg atgcacgact gccccgcctg gtagtagccg ccgaacgcga tccgctcagc
     4561 ggcgtggtcg aggtcggcgt cggagtgcac gatcacaccg gcgttgccgc cgagctcgag
     4621 cgtgaaccgc ttccggggat cgagaccctt cagccgccag cccacgggcc ccgagcccgt
     4681 gaacgaaacc ttcttgatcc ggggatcggt gacgagcggc tccgccttcg atcccgag
//
LOCUS       DASUTZ010000043         2524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1306792, whole genome shotgun sequence.
ACCESSION   DASUTZ010000043 DASUTZ010000000
VERSION     DASUTZ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306792"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..654)
                     /gene="hisB"
                     /locus_tag="VF097_01990"
     CDS             complement(73..654)
                     /gene="hisB"
                     /locus_tag="VF097_01990"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX6261598.1"
                     /translation="MTRAAKAERKTKETSILVTLGVDGSGESSAETGLPFFDHMLEQL
                     GKHARWDLEVRAEGDLEVDAHHTVEDVGLALGEALREALGDKAGIRRFGSAAVPLDEA
                     LVEVALDLSARPYVVHEVPVPAEMIGTYDPGLTQDFVQAFATEAGMTIHCRLISGRSP
                     HHVVEAEFKAMAVALRSACEEIGGGTPSTKGSL"
     gene            complement(651..1718)
                     /gene="hisC"
                     /locus_tag="VF097_01995"
     CDS             complement(651..1718)
                     /gene="hisC"
                     /locus_tag="VF097_01995"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013019219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX6261599.1"
                     /translation="MTKPPLRPDLHSLSAYLAPQIDAPVRLNTNECPYPPPEAFAREL
                     GEAVARIPFHRYPDRDATALRKGLAELTGHPAEGVWAANGSNEVIQHLCLAYGGPGRG
                     AMVFEPTYSLHRLIPRMVGMEIFKARLGDRFLLDERAVESVAGYRPSIVFVCSPNNPT
                     GNAQTADLVAPLCEATDGLVIVDEAYGEFGGATAQGLVTERDNLVVVRTFSKAFSMAA
                     ARLGYALAPPAIVEDLAKVRLPYHLSSLTQAAGEVALRHLEEARALLDRIRDQRDRIL
                     AELGSLEHVEVFPSEANFVLFRTAVEAEALWRALLDRGVLVRDVSAYPGLERCLRVTA
                     GTPEETGAFLEALPEALEEVA"
     gene            complement(1715..>2524)
                     /gene="hisD"
                     /locus_tag="VF097_02000"
     CDS             complement(1715..>2524)
                     /gene="hisD"
                     /locus_tag="VF097_02000"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX6261600.1"
                     /translation="CTPPAQDGSVPDAVLYAARAGAIHEVFRIGGAQAIAAMAFGTET
                     VPSVDTIAGPGNVWVTAAKREVMGTVGVDALAGPTELAIVADGTADPSVLAVDLVAQC
                     EHDPMARTTLVALDPGLPAAIEVPLAAELEASPRGEGVRAALEHAVAVLAPDEEAAAS
                     VVDALAPEHLQVVTADPRAFLARVRSYGAAFLGPHTAVSLGDYGVGSNHVLPTMATAR
                     FASGLRAAHFVTVSSWTEASPEGAARFGPEVETVARAEGLGAHARASEVRR"
BASE COUNT          334 a          954 c          907 g          329 t
ORIGIN      
        1 cgacgcgctc caccgcgcgc tggacggacc tcaggttccc gaagccgtag tcgaggatcg
       61 ccaccgaggt ggtcacaggg accccttcgt ggacggcgtc cccccgccga tctcctcgca
      121 cgcgctgcgc agcgccaccg ccatcgcctt gaactcggcc tcgacgacgt ggtgcgggct
      181 gcggcccgag atcagccggc agtggatcgt catcccggcc tcggtggcga aggcctgcac
      241 gaagtcctgg gtgaggcccg gatcgtaggt cccgatcatc tcggccggca ccggcacctc
      301 gtgcacgacg tagggcctgg cggagaggtc gagcgccacc tcgaccagcg cctcgtccag
      361 cgggaccgcc gcgctgccga accggcggat cccagccttg tcgcccaggg cctcccggag
      421 agcctcgcca agcgcgagcc ccacgtcctc cacggtgtgg tgcgcgtcca cctcgaggtc
      481 gccctccgcc ctgacctcca ggtcccagcg ggcgtgcttc cccagctgct cgagcatgtg
      541 gtcgaagaag ggcaggccgg tctccgcgct gctctcgccg gagccgtcca cgcccagggt
      601 cacgaggatg ctcgtctcct tcgtcttccg ctcggccttc gccgctcgcg tcacgcgacc
      661 tcctccaggg cctcgggcag ggcctccagg aaggcccccg tctcctcggg cgtgcccgcc
      721 gtcacccgga ggcaccgctc cagcccaggg tacgcggaga cgtctcggac gagcacgccc
      781 cggtcgagca gagcccgcca gagcgcctcc gcctcgaccg cggttctgaa cagcacgaag
      841 ttcgcctcgg acgggaatac ctccacgtgc tcgaggctcc cgagctcggc gaggatccgg
      901 tcgcgctggt cgcggatccg gtcgaggagg gcgcgggcct cctcgaggtg gcgcagggcc
      961 acctcgcccg ccgcctgggt cagcgaggag aggtggtagg ggagccggac cttggccagg
     1021 tcctccacga tcgccggcgg cgccagcgcg tagccgagcc gcgcggcggc catcgagaac
     1081 gccttggaga aggtccggac gaccaccagg ttatcgcgct ccgtgaccag gccctgcgca
     1141 gtggcgccgc cgaactcgcc gtaggcctcg tccacgatca cgaggccgtc cgtggcctcg
     1201 cacagcggcg cgaccaggtc ggccgtttgg gcgttccccg tagggttgtt cggggagcac
     1261 acgaagacga tcgatggccg gtagccggcc acggactcga ccgcccgttc gtcgagcagg
     1321 aagcggtctc ccagcctcgc cttgaagatc tccatcccca ccatccgggg gatcagccgg
     1381 tggagcgagt acgtcggctc gaacaccatc gcgccgcgcc cgggtccacc gtaggccagg
     1441 cacaggtgct ggatcacctc gttggagccg ttcgcggccc agaccccctc ggcggggtgt
     1501 cccgtcagct cggccagccc cttccgcagg gccgtcgcgt cgcggtccgg atagcggtgg
     1561 aaggggatgc gggccaccgc ctcgcccagc tcgcgggcga aggcctcggg cggggggtag
     1621 gggcactcgt tcgtgttgag gcgcaccggc gcgtcgatct gcggggccag gtacgcggag
     1681 agcgagtgca ggtcggggcg gaggggcggc ttcgtcatcg gcgcacctcg ctggccctgg
     1741 cgtgggcgcc caggccctcc gccctggcca cggtctcgac ctcgggcccg aaccgcgcgg
     1801 ccccctcggg ggaggcctcc gtccacgagg acaccgtcac gaagtgcgcc gcgcgcaggc
     1861 cggaggcgaa ccgggccgtg gccatcgtgg ggagaacgtg gttcgagccc acgccgtagt
     1921 cgcccagcga gaccgcggtg tgcggcccga ggaacgcggc gccgtaggag cggacccgcg
     1981 ccaggaacgc cctcgggtcg gccgtcacca cctggaggtg ctcgggggcg agggcgtcga
     2041 cgaccgaggc ggccgcctcc tcgtcgggcg cgagcacggc caccgcgtgc tccagcgccg
     2101 cgcggacgcc ctcgccccgc gggctcgcct cgagctcggc cgccaagggc acctcgatcg
     2161 cggccgggag gcccggatcc agggcgacca gcgtggtccg ggccatcggg tcgtgctcgc
     2221 actgggccac gaggtccacg gcgagcaccg acggatcggc cgtcccgtcc gccacgatcg
     2281 ccagctcggt ggggccggcc agggcgtcca cgcccaccgt gcccattacc tcgcgcttcg
     2341 ccgccgtgac ccagacgttg cccgggccgg cgatcgtgtc caccgagggc acggtctcgg
     2401 tcccgaaggc catcgccgcg atcgcctggg cgccgccgat gcggaagacc tcgtggatgg
     2461 cgcccgcgcg cgccgcgtac agcaccgcgt cggggacgct cccgtcctgg gccggcgggg
     2521 tgca
//
LOCUS       DASUTZ010000044        10669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_140581, whole genome shotgun sequence.
ACCESSION   DASUTZ010000044 DASUTZ010000000
VERSION     DASUTZ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10669
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140581"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VF097_02005"
     CDS             <1..796
                     /locus_tag="VF097_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX6261601.1"
                     /translation="FRMIPYEIKTVEELGLPRQIEMFARTPRGMVLLTGPTGSGKSTT
                     LATLVDIVNQERDSHIMTVEDPIEFLHRHKRAMVNQREVGQDTQGFAQALKHVLRQDP
                     DVILIGEMRDLETIQTALTAAETGHLVFATLHTQDAPQTIDRIIDVFPPFQQQQVRVQ
                     LAATLQGVVTQQLLPTADGRGRVVAAEILIATPAVRNLIREAKIHQIYSSMQAGGKLG
                     MQTMDAALAHLVKSGRITYDTAVSQCHSLDELNRLLGKPVVGGNDG"
     gene            789..2000
                     /locus_tag="VF097_02010"
     CDS             789..2000
                     /locus_tag="VF097_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX6261602.1"
                     /translation="MAETYTYRARDAGGALRTGEIQADNDALVMSRLRELGYVPLEVK
                     QKSSGLQREFTLRPGRVKLKDLTVFSRQFATMINSGLPLLRCLSILEQQTSSKELGKI
                     IVQVRTEVEQGSSLSQALSNHPKAFSKLYVAMVRAGETSGTLDDVLLRVADTLEKEVE
                     LRRKVKSAMTYPTVVFALVILILIAMLVFVVPTFETLYEDLGGTLPVPTKVLIAVSQV
                     VRGYFLWVVAGFVLLIWGFRRWILTDKGRRAFDRFKLKLPIFGKLFHKTALSRFSRTL
                     GVLSRSGVPILQALDIVSETVGNSVMADAVRDIGASVKEGESITKPLSRHDIFPPMVV
                     QMMAVGEETGALDTMLEKIADFYDAEVEATVDALTSLIEPLLIAVVGGTVGVIVISLY
                     LPLFKIFELIK"
     gene            2121..2615
                     /locus_tag="VF097_02015"
     CDS             2121..2615
                     /locus_tag="VF097_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX6261603.1"
                     /translation="MLQAIRKRMEDDKGFTLIELMVVVLIIAILIAIAIPTFLGLRQR
                     AQDRAAQSDLRNGLTAAKAWYTDGDTYAGFNETEGEAIEPSLDWVAGGVASTTANLAV
                     SVGVGTTNSADDTVGLSRMSASGTCFHISDVSTNPGAGTFRNSGGGCTGAAAVGMTSS
                     DGSW"
     gene            2753..4462
                     /locus_tag="VF097_02020"
     CDS             2753..4462
                     /locus_tag="VF097_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261604.1"
                     /translation="MLRRLKERDPEAGFTLVELSVALGVLFLALLAMAQTAVVGFRDT
                     GFARQRQTANQIANQLIEEVRGLAYEHVTKGLADEDLAGDPTNIKLCGLEYYFKVCPP
                     DPAAEVIVHTQGLLATPVTPLVPHQGTYGPPDYPSDFDWSVYVTEAKNVPEAGAFRVW
                     AIVTWANPQNPGASNSVETQTLIFSPTGCQDTTTHPFGAPCQAYHQGTAAINGGGSQT
                     VGTAQGVTFDAFMVDMLTLNSTVQEEQVVRVDGSSALPGGRRIVAGVENTVGRVTGTS
                     QADTDPSTPAGPYDDVSLGPQGSESSAVTGTSTTITVASGGGDTGATTAATAANATNP
                     CTVQSDGRACSYAVGRHQGALTHTVRIPEDGGTATLVSVGNNSTPSLAYARRQIPVAG
                     TDGLVRARVERIVPEVRLGGLPDRASAPAGWAGYWARLVNYTATVQVEAGVNTNAPTL
                     VLTGTLEYWNGSGYTSVTVTPGGAVVTPAAVSVPVTGPNFGSGSADLSGTIEIKSSST
                     SQTVGTPNTTRASAEAIAGPPLFAEVSYEIRRGANTVVDLDLFVNLGRAVARTSYEPA
                     PTP"
     gene            4459..5079
                     /locus_tag="VF097_02025"
     CDS             4459..5079
                     /locus_tag="VF097_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261605.1"
                     /translation="MIGWRLARLRKEQSGVTIPEVTIALSVMLLVAGAFLSVVISLQA
                     NLVRQQRRSENNDQARLAIEQLDREIRSGNVLYNPATEADPYYGLRIYTQANAPTRTP
                     SFQCVQWLIEDDQLKRRSWPPNQPELAGSWRVVAEGIVNRTLGEEAFQLDPEPSKGNR
                     TVMIKLTVNGDLPDDPAATIRIQTSLTGRNTSLGFSSDVCADLPAS"
     gene            5093..6484
                     /locus_tag="VF097_02030"
     CDS             5093..6484
                     /locus_tag="VF097_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261606.1"
                     /translation="MQLFMRRANDERGVALLTAVMISAIVFIMGVTAVQIAVHNSDAS
                     ARNRRRVESIGSAEAGLDYYFSHLTDVSPTEIECSVTRQLTGSPTASFTVSAEFFDVA
                     GDPIPCPITDDDIIPDSALITSEGTTSSTDPTRTMQSFVYLVPKPPRAFGDSAIFSDA
                     DPLFNSNVTVFGAGAINADIYTNGDVILQSNVIAEGSVYAQGSAIIEGNADIKKNVWA
                     NDFVELRNQAVVRRNVTSSSSYVSVDSNQSAHIYGDAQAGTTITASANAIDGLRIQNV
                     PSDPPPQREFPDFVFEAQDWLDEGYLITPFADCTLARNFLQALPAGKHVIHITSSCDL
                     RWPSNSTWRVPDDLAVISNGSLTMQSNSRILSSGGPHTLFLIFGLGRTNPCDITFGSN
                     SDVGDGLTTTFYTPCSINMNSNSFTANGQMFGGVVNFNSNATLTFELVPIPGFAPDGF
                     DQDITFIREVRTT"
     gene            6507..7295
                     /locus_tag="VF097_02035"
     CDS             6507..7295
                     /locus_tag="VF097_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020514147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX6261607.1"
                     /translation="MEPGLPLVVRVAIFAVLGLVVGSFLTVVIHRVPRNESIVRPRSR
                     CPRCGAELRARDNLPVVGWLLLRGRCRACGERISPFYPLTELATGALFAGAAFLDPLY
                     AAAVASAFMASMVAVAVIDARHRIVPNRITYTMTPTFAAAIIVGDLAGHGLSILDGLL
                     GMVLYAVPLFLIALALPGGMGMGDVKLVAMIGLVLGAFGLRYVGVAAFLGIVGGGVGA
                     LLAMAVMGYGRKQQIPFGPFLAAGGALALLLGPPIADWYLGLLP"
     gene            7526..7738
                     /locus_tag="VF097_02040"
     CDS             7526..7738
                     /locus_tag="VF097_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016437488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6261608.1"
                     /translation="MMPKRRPFVGDRLLTVGEVAATMRVSNMTVYRLIKAGQLPAIRV
                     GKNYRIRESDVNKYLSDRAVQVEEEG"
     gene            7738..8784
                     /gene="pilM"
                     /locus_tag="VF097_02045"
     CDS             7738..8784
                     /gene="pilM"
                     /locus_tag="VF097_02045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEX6261609.1"
                     /translation="MARTRIGLDIGSTSVRAAELSVGGGVPALVRIAQVPLAPGAVEN
                     GEVRDVEAVTEAVRELWRRGGFKSREASLGVGNQRVVVREVTLPALPEKEMRQSLPFQ
                     VQELIPIPMDEAVLDYDVLEELEEEGRKVHRILVVAAQREMVDRHLAVATGAKLNPVG
                     VDLVPFALVRSIGEHDGLGLEESDAGGEAVVDVGADVTNISVHERGVPRFVRILPSGS
                     NDVTTSVASSLGVDAETAETFKRGGTASDDARRTLEQRVTALVDEIRSSLDFYRAQTP
                     GAQVSRIRTSGGGSKMPGFIELLGQRTGVATEAGRPFSKVDVRVSLSPEDLLEAEPLL
                     AVSIGLALPQEEPR"
     gene            8781..9410
                     /locus_tag="VF097_02050"
     CDS             8781..9410
                     /locus_tag="VF097_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEX6261610.1"
                     /translation="MRINLLPSEIRERQRARRRTAAAAVVGLLVLAAVGAFYFLQQVR
                     LSNVEDELEEQQAVNAELRQDIAELQRFADLEAELLATRGLLDELLKNQVLWSGVLRD
                     VSLVIPGTSWLTALEGSVTAEAAPATGDVQAAPDAQPGIVGQISFTGTAFDHRTVALW
                     LARLEDVGGFINPWLSSSQKSEIGTTEVVDFNSSVDLSEDAVTPPGGRR"
     gene            9407..10036
                     /gene="pilO"
                     /locus_tag="VF097_02055"
     CDS             9407..10036
                     /gene="pilO"
                     /locus_tag="VF097_02055"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEX6261611.1"
                     /translation="MTRRGPILVIVVSLVLAALLFFLLVLPKMREVSRTQDQVEQAEA
                     EELTLRAELARLEGVRENARQIRRQVARFRRAVPPVADLPGLINALQDVADVADVDFF
                     SVAPGTPAAFPQGTASEIPAQIQVIGGFFQVDEFLFRLETMDRAAKVITISVGPGPDE
                     LPQIQASLSVEFYTTDPAAGPGTEPEVIEESPSPGASPTPTPTASPTEG"
     gene            10040..10639
                     /locus_tag="VF097_02060"
     CDS             10040..10639
                     /locus_tag="VF097_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261612.1"
                     /translation="MSPRERNLLIVLGVVALAAVAFFFLVVRPGGAPDETAPTLGASP
                     SPDLSPSPEPEEEEEEEGELPRPRTPGIAFFSGRDPFMPLVEDTTTTTNGGTTTTNGD
                     DGGTTTPPPGNGDGDDGNGGVPSEQRGESITVGGHTVTLIDILAIGRVQVEVDGQTYT
                     VSPGETFADNFQLVSVQGGCANFRFGDQSFTLCEPGERK"
BASE COUNT         1726 a         3546 c         3701 g         1696 t
ORIGIN      
        1 gttccggatg atcccctacg agatcaagac cgtggaggag ctcgggctgc ctcggcagat
       61 cgagatgttc gccaggaccc cgaggggcat ggtcctgctc accggaccga ccggctccgg
      121 gaagtcgacc acgctggcca ctctcgtgga catcgtgaac caggagcggg actcgcacat
      181 catgaccgtc gaggacccca tcgagttcct ccaccgccac aagagggcga tggtgaacca
      241 gcgagaggtc gggcaggaca cgcaggggtt cgcccaggca ctgaagcatg tcctgcggca
      301 ggaccccgac gtcatcctca tcggtgagat gcgcgacctg gagacgatcc agaccgcgct
      361 gaccgccgcc gagaccgggc acctcgtctt cgcgaccctg cacacgcagg acgcgcccca
      421 gacgatcgac cggatcatcg acgtgttccc gccgttccag cagcagcagg tccgggtgca
      481 gctcgcggcg accctgcagg gcgtcgtgac ccagcagctg ctgccgaccg cggacgggcg
      541 gggacgggtc gtggcggccg agatcctcat cgccacgcct gccgtccgga acctgatccg
      601 cgaggcgaag atccaccaga tctactcgtc gatgcaggcg ggggggaagc tcggcatgca
      661 gacgatggac gcggcgctgg cgcacctggt gaagtcgggc cggatcacct acgacacggc
      721 agtcagtcag tgccacagcc tggacgagct gaacaggctc ctcgggaagc ctgtggtggg
      781 aggcaacgat ggctgagacc tacacgtacc gtgcacggga tgcgggcggg gctctgcgga
      841 cgggcgagat ccaggccgac aacgacgccc tggtgatgtc tcgcctccga gagttgggct
      901 acgtcccgct cgaggtgaag cagaagagct cggggctcca gcgggagttc acgctgcgcc
      961 cgggccgcgt gaagctgaag gacctcacgg tcttcagcag gcagttcgcc acgatgatca
     1021 actccgggct tcccctcctg cggtgccttt cgatcctgga gcagcagacg tcgagcaagg
     1081 agctggggaa gatcatcgtc caggtccgca ccgaggtgga gcaggggagc tcgctctccc
     1141 aggcgctctc caaccacccg aaggcgttct cgaagctcta tgtggcgatg gtgcgggccg
     1201 gcgagacgag cggcacgctg gacgacgtcc tgcttcgggt cgccgacacc ttggagaagg
     1261 aggtcgagct tcgccgcaag gtgaagtcgg cgatgaccta cccgaccgtg gtcttcgcgc
     1321 tcgtcatcct gatcctcatc gcgatgctgg tgttcgtggt cccgaccttc gagacgctgt
     1381 acgaggacct gggcggcacc ctgccggtgc cgacgaaggt gctgatcgcg gtgtcgcagg
     1441 tcgttcgggg ctacttcctg tgggtggtgg ccggcttcgt gttgctgatc tggggcttcc
     1501 gtcggtggat cctcaccgac aaggggcggc gggcgttcga tcgcttcaag ctgaagctcc
     1561 cgatcttcgg gaagctgttc cacaagacag cgctgtcgcg gttctcgcgg accctcggcg
     1621 tcctctcgcg ttcgggcgtc ccgatcctcc aagcgctgga catcgtctcg gagaccgtgg
     1681 gcaactcggt gatggccgac gcggtccggg acatcggggc gagcgtcaag gaaggggaga
     1741 gcatcacgaa gccactctcc aggcacgaca tcttcccgcc catggtcgtc cagatgatgg
     1801 cggtggggga ggagaccggc gccctggaca cgatgctgga gaagatcgca gacttctacg
     1861 acgcggaggt cgaggccacg gtagacgccc tgacgtcgct gatcgagccg ctgctgatcg
     1921 cggttgtcgg tggaaccgtg ggcgtcatcg tgatctcgct ttacctgccg ctgttcaaga
     1981 tcttcgagct gatcaagtaa cgacgcatca tcctcctggg aggcggggcc ctcgggcccc
     2041 gcctccaact ttttcctcaa ccttcgaccg atcgtgccga tgctcatgag gagactgcag
     2101 tagagcctta ggaggtgaac atgctgcagg cgattcgcaa gaggatggag gacgacaagg
     2161 gcttcacgct catcgaactc atggtcgtcg tcctcatcat cgcgatcctg atcgcgatcg
     2221 cgatccccac gttcctgggg cttcggcagc gggcgcagga ccgggccgcg cagtcggacc
     2281 ttcggaacgg cctcacggcc gccaaggcct ggtacacgga cggggacacg tacgccgggt
     2341 tcaacgagac cgagggcgag gccatcgagc cctctctcga ctgggtcgcc ggcggcgttg
     2401 cgtccacgac cgcgaacctg gcggtcagcg ttggcgtcgg taccacgaac agcgccgatg
     2461 acacggtggg cctgtcccgg atgtccgcgt cggggacgtg cttccacatc tcggatgtct
     2521 cgacaaaccc gggtgcgggc acgttccgga actccggcgg cggatgcaca ggggccgctg
     2581 cggtgggcat gacctccagc gacggcagct ggtaagggat cgctgcaaga ccctggaaga
     2641 gggcggcctt cgcgccgccc tcttcctctt ttccgcttag gtcggccgac cgaccgaccg
     2701 atagataatc gaggagcggc gaccgccgtt ctccaccgac gctgggatga ccatgctgcg
     2761 aaggctgaag gagcgggatc cggaggcggg gttcacgctg gtcgagctct ccgtcgcgct
     2821 gggcgtgctc ttcctcgccc tgttggcgat ggcccagacc gccgtggtcg ggttccgcga
     2881 cacgggcttc gcccgccagc gccagacggc caaccagatc gccaaccagc tcatcgagga
     2941 ggttcgcggc ctcgcctacg agcacgtgac caaggggttg gccgacgagg atcttgcagg
     3001 cgacccgacg aacatcaagc tgtgcggact cgagtactac ttcaaggtgt gcccgccgga
     3061 cccggccgcc gaggtgatcg tgcacacgca ggggctgctg gccactcccg ttacaccgct
     3121 cgtcccccat caggggacgt acggaccgcc cgactacccc tcggatttcg actggagcgt
     3181 gtacgtgacc gaggccaaga acgtgcccga ggccggcgcc ttccgcgtgt gggccatcgt
     3241 cacctgggcg aatccgcaga accccggagc cagcaactcc gtggaaaccc agacgctcat
     3301 cttctcgcct accgggtgcc aggacaccac gacccacccg ttcggggctc cctgtcaggc
     3361 ctatcaccag ggaacggcgg cgatcaacgg cggcggatca cagacggtgg gcacggcgca
     3421 gggcgtgacc ttcgacgcgt tcatggtgga catgctgacg ctgaactcga cggtgcagga
     3481 ggagcaggtg gtgcgggtcg acggatcctc cgcgctgcca ggcggtcggc gcatcgtggc
     3541 cggtgtggag aacaccgttg ggcgcgtcac cggcaccagc caggcggaca ccgacccgtc
     3601 caccccggcc ggcccctacg acgacgtctc cctcggcccg caggggagcg agtcgtccgc
     3661 ggtgacgggg acgagcacga cgatcacggt tgcgtccggg ggtggcgaca ccggcgcgac
     3721 caccgcggct acggccgcga acgccactaa cccgtgcacc gtgcagtcgg acggacgagc
     3781 gtgctcctac gccgtgggcc gtcatcaagg cgcgctcacc cacaccgttc ggatccccga
     3841 ggacggtggc acggccacgc tcgtcagcgt gggaaacaac agcacaccgt cgttggccta
     3901 cgctcggagg cagatcccgg tcgccgggac cgacgggctc gtgcgagctc gcgtcgagcg
     3961 gatcgtcccc gaggtgcggc tgggcggtct tcccgaccgc gccagcgccc cggccggatg
     4021 ggccggctac tgggcccggc tggttaacta caccgccact gtccaggtgg aggccggcgt
     4081 aaacaccaac gccccgacgc tggtcctgac cggaacgctc gagtactgga acggctcggg
     4141 gtacacgagc gtcacggtga cgcccggagg agccgtcgtc acgcctgcgg ccgtcagcgt
     4201 ccccgtcacc ggaccgaact tcggaagcgg cagcgcggac ctgtccggga cgatcgagat
     4261 caagtcctcg tcgacgtcgc agaccgtggg cacgcccaac acgacccgcg ccagcgccga
     4321 ggcgatcgcg ggccctccgc tcttcgcgga ggtcagctac gagatccgcc ggggcgcgaa
     4381 cacggtcgtc gacctcgacc tgttcgtcaa cctgggccgc gccgtggccc gaacctccta
     4441 cgagcccgcc ccgacgccat gatcggctgg aggctggctc ggctccgcaa ggagcagtcg
     4501 ggggtcacga tcccggaggt cacgatcgcc ttgtcggtga tgctcttggt cgccggggca
     4561 ttcctctcgg tcgtcatctc gctccaggcg aacctcgtgc gacagcagcg gcgcagtgag
     4621 aacaacgacc aggcccgtct cgcgatcgag caactcgatc gcgagatcag gtcgggcaac
     4681 gtgctttaca accccgccac ggaagcggat ccgtactacg ggctccgcat ctacacgcag
     4741 gcgaacgcgc cgacgcggac cccgtccttt cagtgcgtcc aatggctgat cgaggacgac
     4801 cagctgaagc ggcggagctg gcctcccaac cagcccgagc tggcgggctc atggcgggtc
     4861 gtcgccgagg gcatcgtcaa ccggacgctg ggagaggagg cgttccagct cgatcccgag
     4921 ccaagcaagg gcaatcgcac agtgatgatc aagctgaccg tgaacggaga ccttcccgac
     4981 gacccggcgg ccacgatcag gatccagacc tcgctgaccg gccggaacac gtcgctgggg
     5041 ttctcttcgg acgtgtgcgc cgacctgccg gcctcgtgac gtaggaggag agatgcagct
     5101 gttcatgcgg agggcgaatg acgagcgagg ggtggcgctc ctcacggcgg tcatgatctc
     5161 ggccatcgtg ttcatcatgg gtgtgacggc cgttcagatc gccgtgcaca acagcgacgc
     5221 gagcgcacgc aaccgccgcc gggtggagtc gatcggctcg gctgaggcag gcctcgacta
     5281 ctacttctcg cacctcacgg atgtctcgcc cacggagatc gagtgctcgg tcacgcggca
     5341 gctgacgggg tctcccacgg cctccttcac cgtcagcgct gagttcttcg acgtcgcggg
     5401 cgatcccatc ccctgcccca tcaccgacga cgacatcatc cccgactccg cgctcatcac
     5461 gtcggagggg accacgagct cgacggatcc cacccggacg atgcagtcgt tcgtctacct
     5521 ggtccccaag cccccccggg cctttggcga ctcggcgatc ttctcggacg cggatcccct
     5581 cttcaactcc aacgtgacgg tgtttggggc tggagcgatc aacgcggaca tctacacgaa
     5641 cggcgacgtg atcctgcaga gcaacgtgat cgccgagggc agcgtctacg cgcaggggag
     5701 cgccatcatc gaggggaacg ccgatatcaa gaagaacgtg tgggcgaacg atttcgtgga
     5761 gctccgcaac caggccgtgg ttcgacggaa cgtcacgtcg tcgtcctcgt acgtctcggt
     5821 cgactcgaat cagtcggcac acatctacgg cgatgcccag gccggcacga cgatcaccgc
     5881 gagcgcgaac gcgatcgacg gactgcggat ccagaacgtc ccttcggatc ctccgccgca
     5941 gcgggagttt cccgacttcg tcttcgaggc gcaggactgg ctcgacgaag gctacctgat
     6001 cacgcccttc gcggactgca cgctcgcccg caacttcctc caggcgcttc cggccggcaa
     6061 gcacgtcatc cacatcacgt cgtcctgcga cctccggtgg ccgtcgaaca gcacatggag
     6121 ggtgccggac gacctcgcgg tcatcagcaa cgggtcgctc acgatgcaga gcaactcacg
     6181 gatcttgagc agcggcggtc cacacaccct gttcctgatc ttcgggctgg gcaggacgaa
     6241 tccatgcgac atcacgttcg gctccaactc ggacgtgggg gacggcctca ccacgacctt
     6301 ctacacgccg tgcagcatca acatgaactc caactcattc acggcgaacg ggcagatgtt
     6361 cgggggagtg gtcaacttca actcgaacgc cacgctgacg ttcgagctgg tcccgatccc
     6421 cgggttcgcg cccgacgggt tcgaccagga catcaccttc atccgcgagg tccggacgac
     6481 gtaggctcac gccgggaggg gacccgatgg agcctggcct gccgctcgtc gtgcgcgtcg
     6541 cgatcttcgc ggtcctgggg ctggtcgtgg gctccttcct cacggtggtg atccaccgcg
     6601 tcccgaggaa cgagtcgatc gtgcggccgc gctcgaggtg cccccgatgc ggcgccgagc
     6661 tccgggcccg cgacaacctt cccgtcgtcg gctggctgct gctgcgggga cggtgccggg
     6721 cctgcggcga gcggatctcg ccgttctatc cgctgaccga gctggcgacg ggagctctct
     6781 tcgcgggggc cgccttcctc gatcctttgt acgcggcggc ggtggcgtcg gccttcatgg
     6841 catcgatggt ggcggtggcc gtgatcgacg cccgccaccg gatcgtaccc aaccggatca
     6901 cctacacgat gaccccgacg ttcgcggcgg cgatcatcgt cggcgatctg gccgggcacg
     6961 gcctcagcat cctcgacggc ctgctgggaa tggtcctgta cgccgtgccc ctgttcctca
     7021 tcgccctggc cctcccgggt gggatgggga tgggggacgt gaagctcgtc gccatgatcg
     7081 gcctggtcct gggggcgttc ggcctgcggt acgtgggcgt ggccgccttc ctcggcatcg
     7141 tcgggggagg ggtgggggcg ctcctggcga tggcggtgat ggggtacggc aggaagcagc
     7201 agatcccttt cgggccgttc ctggccgcgg ggggagcgct cgctctcttg ctcgggccgc
     7261 cgatcgcgga ctggtatctc ggtctgctgc cctgatcgcg gcataacaac acctttgtca
     7321 ttccccgagg cgtcgttaaa tgacagtgat gtggttctac atgcgttaaa gattgtcgga
     7381 cgcaatgccg attgacaagg ggtgggtcag ttgttaaggt tcgcatcctt cgtgatggat
     7441 gagcaggaag aagcacgaga gagcggtgtg acggatggtg aacatgtggc aagcgttgtc
     7501 gttggtgagc ttggggagga tgtgaatgat gcccaagcgc cgcccgttcg tgggtgaccg
     7561 gctcctgacg gtgggagagg tggccgcgac gatgcgcgtg tccaacatga cggtctaccg
     7621 cctcatcaag gcgggtcagc tgccggcgat ccgggtcggg aagaactacc ggatcaggga
     7681 gtcggacgtg aacaagtacc tctctgatcg ggcggttcag gtcgaggagg agggctagtg
     7741 gctcgcacga ggatcggcct ggacatcggg tccacgagcg tccgagcggc cgagctttcg
     7801 gtcggcggcg gcgtcccggc gctggtgcgc atcgcccagg tccccctggc tcccggcgcc
     7861 gtcgagaacg gcgaggtccg cgacgtagag gcggtgaccg aggcggtccg cgagctgtgg
     7921 cggcggggcg gcttcaagag ccgcgaggcg tcgctcggcg tggggaacca gcgggtcgtc
     7981 gtccgcgagg tgaccctgcc ggcgctcccc gagaaggaga tgcgccagtc gctgccgttc
     8041 caggtccagg agctgatccc gatcccgatg gacgaggcgg tgctcgacta cgacgtcctc
     8101 gaggagctgg aggaggaggg ccggaaggtc caccggatcc tcgtcgtggc cgcccagcgc
     8161 gagatggtgg accggcacct cgcggtcgcc accggcgcga agctgaaccc cgtgggcgtg
     8221 gacctcgtcc cgttcgccct cgtgcgctcg atcggcgagc acgacggcct ggggctcgag
     8281 gagtccgacg ccgggggcga ggccgtcgtg gacgtcggcg cggacgtgac gaacatcagc
     8341 gtgcacgagc gtggcgtccc gcggttcgtg cggatcctcc catcgggctc caacgacgtg
     8401 accacgtcgg tcgcctcgtc gctcggcgtg gacgccgaga cggcggagac ctttaagcgc
     8461 ggcggtacgg cgtcggacga cgcccgccgc acgctcgagc agcgggtgac ggcgctcgtg
     8521 gacgagatcc gctcgtcgct cgacttctac cgggcgcaga cgccgggggc ccaggtctcc
     8581 cggatccgga cgtccggcgg cgggtcgaag atgcccggct tcatcgagct cctcgggcag
     8641 cgcaccggcg tggccactga ggcggggcgc cccttctcca aggtggacgt ccgggtctcg
     8701 ctgagccccg aggacctcct ggaggccgag ccgctgctcg cggtctcgat cgggctcgcc
     8761 ctgccgcagg aggagcctcg atgaggatca acctccttcc ctcagagatc cgtgagcgcc
     8821 agcgcgcccg gcgtcgcacg gcagcggccg cggtcgtggg gctgctggtc ctcgcggcgg
     8881 tgggcgcctt ctacttcctg cagcaggtgc gactgtccaa cgtcgaggat gagctcgagg
     8941 agcagcaggc ggtcaacgcc gagcttcggc aggacatcgc cgagctccag cggttcgccg
     9001 acctcgaggc agagctgctg gcgacgcgcg ggctgctcga cgagctgctg aagaaccagg
     9061 tcctgtggtc gggggtcctg cgtgacgtct ccctcgtgat cccggggacc tcgtggctga
     9121 cggctctcga ggggtcggtg acggccgagg ccgcccccgc gacgggcgac gtccaggccg
     9181 cgccggacgc gcagccgggg atcgtgggtc agatcagctt cacggggacc gccttcgatc
     9241 accggacggt ggccctgtgg ctggctcggc tggaggacgt gggcggcttc atcaacccgt
     9301 ggctcagctc ctcgcagaag agcgagatcg ggacgacgga ggttgtggac ttcaacagct
     9361 cggtggatct gtccgaggac gccgtgacac ctccgggagg tcgacgatga ctcgccgtgg
     9421 tcccatcctc gtgatcgtgg tcagcctcgt cctggccgcc ctcctgttct tcctgctcgt
     9481 gttgccgaag atgcgcgagg tgtcccggac ccaggaccag gtcgagcagg ccgaggcgga
     9541 ggagctcacg ctccgggccg agctggcccg cttggagggc gtccgggaga acgcgcggca
     9601 gatccgccgc caggtggccc ggttccgccg ggccgtgccc ccggtggccg acctgccggg
     9661 tctgatcaac gcgctccagg acgtcgcgga cgtcgcggac gtggacttct tctccgtggc
     9721 gccggggacg ccggcggcgt tcccgcaggg caccgcttcg gagatccccg cccagatcca
     9781 ggtgatcggc ggcttcttcc aggtggacga gttccttttc cggctggaga cgatggatcg
     9841 agcggcgaag gtcatcacca tctcggtggg gcccggcccc gacgagctgc cgcagatcca
     9901 ggcgagcctg tccgtggagt tctacacgac cgacccggct gccggtccgg ggaccgagcc
     9961 ggaggtgatc gaggagagcc cctcgcctgg cgcctcgccc accccgaccc cgaccgcttc
    10021 gccgacggag ggctgagcga tgtctccgcg tgagcgcaac ctgctgatcg tcctgggggt
    10081 cgtggccctg gcggcggtgg ccttcttctt cctcgtggtc cggcccggtg gggcgcccga
    10141 cgaaaccgcc cccacgctcg gcgcctcacc gagcccggac ctctccccgt cgcccgagcc
    10201 ggaggaagag gaagaggagg agggtgagct gcctcgcccc cggaccccgg ggatcgcgtt
    10261 cttctcgggc cgggacccct tcatgcccct ggttgaggac acgacgacga cgacgaacgg
    10321 cggcacgacc acgacgaatg gggacgacgg cggcacgacg accccgcctc ccgggaacgg
    10381 tgacggcgac gatgggaacg gcggcgtgcc gagcgagcag cgaggcgagt cgatcaccgt
    10441 cggggggcac accgtgacgc tgatcgacat cctggcgatc ggacgggtcc aggtcgaggt
    10501 ggacgggcag acgtacacgg tgtcgccggg agagacgttc gccgacaact tccagctcgt
    10561 ctcggtgcag ggagggtgcg ccaacttccg gttcggcgac cagtccttca cgctctgcga
    10621 gccgggagag aggaagtgac gcccccgcgc ggcccgatgg gacgcggcc
//
LOCUS       DASUTZ010000045         5047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1825122, whole genome shotgun sequence.
ACCESSION   DASUTZ010000045 DASUTZ010000000
VERSION     DASUTZ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1825122"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VF097_02065"
     CDS             <1..187
                     /locus_tag="VF097_02065"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX6261613.1"
                     /translation="AVDELAPHLREAFVLIEVMGLSYRETAVVLGVPPGTLKSRMHAA
                     RKALMESLAFEEDADEV"
     gene            177..2057
                     /locus_tag="VF097_02070"
     CDS             177..2057
                     /locus_tag="VF097_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261614.1"
                     /translation="MRCDAAERELSAALDGEPFDAAARAHVETCAACRRFEAQARRLR
                     DAVRLEPAPAVPDLVGPIMERVRAEPARSGWGREMAAFAAAAVVVALVAGGLPGVRRG
                     PVPVEASEISQRVAEASREITSYRAVFEITERSFADDVPLRRFEARVTFRAPERFLAE
                     VRDRTAYPGDAWPRNDVRLAVDGGRWSLTAPVPCPREALPACASGETRTLAVRGRPPF
                     DGDAPIPTDVILPMKALAGAGRLRVVGESSVSGRSVVRVELDYRDATPLLAFLQAGGS
                     WRPFFPLDRVLVSLDAETWFPLGYRVLAAGSAEREAWALRNGLPRERDGATLLDVRAR
                     TFVESVPEGWRPSVEGSGAVDRGFREAGRGELPFEPAEPATLAGLEPYREGLLGPDEI
                     LTSYARGLSWLRIRESRSWDQEALFGDVGPLAAPVRLPGGGLAYHEPPTDDLGHRLAI
                     HAADTDLYIESNLPFDRLLEMAASVPVRGLPIPDAWRTRRLPGGVQEVGVPLKRAVRV
                     FPGLALPRWLPAEYRLWTLHLFHARSGAAVTTYFRRPGGELDGVGIRLHQAEGTGLPP
                     PTDPGAVGVRIDAEVGRYSPIRGELEWVEDGIYRSLRAEGLDLDHLVAIARSMTPPGG
                     GT"
     gene            2054..3232
                     /locus_tag="VF097_02075"
     CDS             2054..3232
                     /locus_tag="VF097_02075"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX6261615.1"
                     /translation="MRSLRVLVAALVGALVGGAVTLGMPLEDSRPPARRPPPAQLVKQ
                     APIRTLLVWTPGGLPPGYAEAVQRLPSVRAAAEVRSGTAWLTAWRPEGSPRRTPPSGL
                     AYPTEVAAFRPLSYRRFVPPADRPLVESLARGGALLSRGAARLRGIEAGGTLRFGGLG
                     LPVVGVLDDELVGAHEVVVSHETGARLGISRARYLLVAPRAKAERREVEEGIRGALPP
                     GVRVRVRGPGETPVFRHGDAVLPPLRLKELFGEFAAAPAAGGMLRMEGGWVRRNIRTT
                     TLPVVGPVTCHRTIVPAMRTAFAELQRRALDDLIDPGDFGGCYSPRFILRSPGAGISH
                     HGWGIAFDLNVSQNPFGREPRMDPRVVEVFERHGFTWGGRWLVPDGMHFEFLRFAEGD
                     "
     gene            3524..4285
                     /locus_tag="VF097_02080"
     CDS             3524..4285
                     /locus_tag="VF097_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261616.1"
                     /translation="MDWSIVRQAMGTQEPRDWWREPDVAIPTGRVDVWGGSRSFALIR
                     VHFDVPGVEPGTYSLMLCSAGCVEPLGDILPSRVTVAADPVAARTAREVGLLTMQGRY
                     LQNRVGRIQRQIRAMRSADQEMLPMMDGLTTRADEQARRIRELEARVEELEGAAPAPA
                     WLVVGAAGAALLLLLPRRRRPRRPPPEPLVEMKLEALPDPLDDLEPIGERAPVPAADA
                     QPAARPLTPVRARAAAPHRAATARAVSGASPFASP"
     gene            complement(4177..>5047)
                     /locus_tag="VF097_02085"
     CDS             complement(4177..>5047)
                     /locus_tag="VF097_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015108491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX6261617.1"
                     /translation="STSGSPEPLVADERVTPLRVAVVGHVEWVEFVRVDRVPSAGDIA
                     HGLESWEEAGGGGGVSAVQLAMLAGSSTLFTALGDDEVAGEARKSLERSGVRVEATSR
                     RAPTRRAITMLDPGGERTITTVGERLSPMGSDGLPWDELEAADGVFFTAGDEEALRAA
                     RRARVLVATSRVLEEIRRWRVPIDALVGSARDPLETFDVGDLDAPPRLAVLTEGVRGG
                     RWWTADEGWSRYEAAALPGPGGDAYGAGDSFAAGLTYGLAKGLAPDTALAVAARCGAA
                     ARARTGVSGRAAG"
BASE COUNT          599 a         1849 c         1907 g          692 t
ORIGIN      
        1 cgccgtggac gagctcgccc cgcacctccg ggaggccttc gtcctgatcg aggtgatggg
       61 gctgagctac cgggagacgg ccgtcgtgct gggcgtgccg cccggcacgc tgaagagccg
      121 gatgcacgcc gcgaggaagg ctctgatgga gagcctcgcc ttcgaggagg acgccgatga
      181 ggtgtgacgc cgccgagcgc gagctttcgg ccgccctgga cggtgagccg ttcgacgcgg
      241 cggcacgcgc gcacgtcgag acgtgcgccg cgtgccgccg cttcgaggcc caggcccgtc
      301 ggctccggga tgcggtccgt ctcgagcccg ccccggcggt gcccgacctc gtgggcccga
      361 tcatggagcg ggtccgggcg gagccggctc ggagcgggtg gggaagggag atggccgcgt
      421 tcgccgccgc cgcggtggtg gtggccctcg tggcgggagg cctgccgggg gtccggagag
      481 ggcccgttcc cgtcgaggcg tccgagatct cccagcgggt ggccgaggcc tcgcgcgaga
      541 tcacctccta ccgagcggtg ttcgagatca ccgagcggag cttcgcggac gacgtgccgc
      601 tgcgccgttt cgaggcccgc gtcacgttcc gggccccgga gcggttcctg gccgaggtgc
      661 gggaccgcac cgcctacccc ggcgacgcct ggccccggaa cgacgtgagg ctggcggtcg
      721 acggaggacg ttggtcgctg accgcgcccg ttccgtgccc gcgggaggcc ctgcccgcct
      781 gcgcctcggg cgagacgagg acgctggccg tcaggggtcg gccgccgttc gacggcgacg
      841 cgccgatccc cacggacgtg atcctcccca tgaaggcgct cgcgggcgcg gggaggctgc
      901 gcgtcgtggg cgagagctcg gtctccggcc ggtccgtggt ccgggtcgag ctcgactacc
      961 gggatgcgac gccgctgctc gccttcctcc aggcgggggg atcctggcgc cccttcttcc
     1021 cgctggaccg cgtgctggtc tcactcgatg ccgagacgtg gttccccctg ggataccggg
     1081 tgctggccgc ggggagtgcg gagcgagagg cttgggccct ccgcaacggc ctccctcggg
     1141 agcgggacgg cgcgacgctg ctggacgtgc gggccaggac cttcgtcgag tcggtgcccg
     1201 aggggtggcg acccagcgtc gaggggtcgg gagcggtgga tcggggcttc cgggaggcag
     1261 gccggggcga gcttcccttc gagcccgccg agccggcgac ccttgcgggc ctcgagccgt
     1321 accgcgaggg gctcctgggg ccggacgaga tcctgacctc ctacgcccgg ggcctcagct
     1381 ggctccggat ccgtgagagc cggtcgtggg atcaggaggc gctgttcggc gacgtcggcc
     1441 ccctcgccgc cccggtgcgt ctgcccggcg gcggcctcgc ctaccacgag cccccgacgg
     1501 acgacctcgg ccaccggctg gcgatccacg cggccgacac ggacctctac atcgagtcca
     1561 acctcccctt cgaccggctg ctggagatgg ccgcctcggt gccggtgcgc gggctcccca
     1621 tcccggatgc ctggcggacc cgtcgcctcc cgggtggcgt ccaggaggtc ggggtcccgc
     1681 tgaagcgggc cgtccgggtc ttcccgggcc tcgccctgcc gcgatggctg cccgccgagt
     1741 accgcctctg gaccctgcac ctgttccacg ccaggagcgg agcggccgtg accacctact
     1801 tccgccggcc cgggggcgag ctcgacggcg tggggatccg cctccaccaa gctgagggga
     1861 cgggcctccc tccccccacc gacccgggag cggtcggggt ccggatcgac gccgaggtcg
     1921 gcaggtactc accgatccga ggcgagctgg agtgggtcga ggacgggatc taccgctcgc
     1981 tgcgcgcgga gggcctcgac ctggaccacc tcgtggccat cgcgcggtcg atgacgcccc
     2041 ccggaggcgg gacgtgaggt cgctccgagt cctcgtggcc gccctcgtcg gagcgctggt
     2101 gggcggcgcc gtgacgctgg ggatgccgct ggaggactct cgcccgccgg ctcgccggcc
     2161 gccaccagcc cagctcgtga agcaggctcc gatccgcacc ctcctggtct ggacgcccgg
     2221 tggcctcccc cccggctacg ccgaagccgt gcagcgcctg ccgtccgtgc gggcggcggc
     2281 cgaggtccga agcggcaccg cgtggctcac cgcctggagg ccggaggggt cgccgcgccg
     2341 aacgcctcct tcgggcctgg cctacccgac cgaggtggcg gcgttccggc cgctctcgta
     2401 ccggcgcttc gtcccccccg ccgaccggcc cctcgtcgag tcgctggccc gaggcggggc
     2461 gctcctgagc cgcggggcgg cccggctgcg aggcatcgag gccgggggca ccctgcgctt
     2521 cggcgggctg ggcctcccgg tggtaggggt gctggacgac gagctcgtag gggcgcacga
     2581 ggtggtggtc tcccacgaga ccggtgcccg gctagggatc tcccgcgcgc gctacctgct
     2641 cgtcgcgccg cgagcgaagg ccgagcggag agaggtcgag gaggggatcc gcggggcgct
     2701 cccgcctggg gtgcgggtgc gggtgcgagg tcccggggag acgccggtct tcaggcacgg
     2761 cgacgcggtg ctgccgcctc tgcgcctgaa ggagctgttc ggcgagttcg cggcagcgcc
     2821 ggcggcgggg gggatgctga ggatggaggg cggctgggtg cgaaggaaca tccggaccac
     2881 gacgcttccg gtcgtcggcc ccgtcacctg ccaccggacg atcgtgccgg cgatgcgcac
     2941 ggccttcgcg gagctgcagc gacgggccct cgacgatctc atcgatccgg gtgacttcgg
     3001 cggctgctac tccccgcggt tcatccttcg gagccccggt gccggcatct cgcatcacgg
     3061 ctgggggatc gccttcgacc tcaacgtctc gcagaacccg ttcgggcggg agccccggat
     3121 ggatcctcgc gtcgtcgagg tcttcgagcg ccacggcttc acctggggag ggcggtggct
     3181 ggtgccggac gggatgcact tcgagttcct caggttcgcc gagggcgact gaaccatcgg
     3241 gggctcccgc gcgtcggtag ggcagagctt gaggaaggag gcgctcatgc gaccgctgcc
     3301 ccgaacgatg acatcaatcg cggtcacggc cctggtgctg gttgtcgccc agcctgttcg
     3361 tgcgggaggg ggatggggat cctccattcc actgtctggg gacgagctcg ggatcggaga
     3421 gcgcttcacg acgagtcgga gctcggttct cttcgattca gtcgaggcgg cgacgcgagc
     3481 ccgaggcggg accgttcctt actacgccta cctgatcccc gagatggact ggagcatcgt
     3541 gcgccaagcg atgggcaccc aggagccacg cgactggtgg agagagcccg acgtcgcgat
     3601 ccccacggga agggttgacg tgtggggagg ctcccgcagc ttcgcgctga tccgcgtcca
     3661 cttcgacgtt cctggcgtcg agccgggaac gtactcgctg atgctatgta gcgcgggctg
     3721 tgtggagccg ctgggcgaca tcctgccgag tagggtcacc gtggcggcgg atcctgtcgc
     3781 ggcccggacc gcccgggagg ttgggctgtt gaccatgcag ggccgctacc tgcagaaccg
     3841 cgtgggccgg atccagcggc agatccgtgc catgcgctcc gccgaccagg agatgctccc
     3901 gatgatggac ggcctcacga ctcgagccga cgagcaggcc cggaggatcc gggagctcga
     3961 ggcccgggtc gaggagctgg agggggcggc gccggcgccg gcctggctgg tggtgggcgc
     4021 cgcgggtgcc gcgctcctgc tgctcctccc gcgccggcgc cgcccccgcc ggcccccgcc
     4081 cgagcccctg gtcgagatga agctcgaggc gctgcccgac ccgctcgacg acctggagcc
     4141 gatcggggag cgggccccgg tcccggcggc cgacgctcag ccggcggcgc ggccgctcac
     4201 gcccgtccgg gcgcgcgcgg ccgcgccgca tcgtgcggcc accgccaggg ccgtgtcggg
     4261 ggccagcccc ttcgccagtc cgtaggtcag ccccgcggcg aaggagtcgc cggctccgta
     4321 ggcgtcgccc cccggccccg gcagcgcagc ggcctcgtac cggctccacc cctcatccgc
     4381 ggtccaccat cggccgccgc gaacgccctc ggtcaggacg gcgagcctcg gaggcgcgtc
     4441 gaggtcgccc acgtcgaagg tctccagggg gtcgcgggcg ctccccacca gggcgtcgat
     4501 cggcacccgc cagcgccgga tctcctccag cacccgcgac gtcgccacca ggacgcgggc
     4561 ccggcgcgcg gctcgcagcg cctcctcgtc ccccgcggtg aagaagacgc cgtccgccgc
     4621 ctcgagctcg tcccacggca ggccgtccga gcccatcggt gagaggcgct cgcccacggt
     4681 ggtgatcgtc cgctctcccc ccgggtcgag catcgtgatg gcgcggcggg tgggggcccg
     4741 gcggctcgtg gcctcgaccc ggacccccga ccgctccagg ctcttccggg cctcccccgc
     4801 cacctcgtcg tcccccaggg ccgtgaagag ggtggaggag ccggccagca tagcgagctg
     4861 caccgcggac acgccgcccc cgccgccggc ctcctcccag ctctccaggc cgtgcgcgat
     4921 gtccccggcc gatggcacgc ggtccacgcg cacgaactcc acccactcca cgtggcccac
     4981 gacggccacg cggagcgggg tcacccgctc gtcagcgacg agcggctccg gggatcccga
     5041 ggtcgac
//
LOCUS       DASUTZ010000046         6475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1695462, whole genome shotgun sequence.
ACCESSION   DASUTZ010000046 DASUTZ010000000
VERSION     DASUTZ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6475
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1695462"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VF097_02090"
     CDS             <1..279
                     /locus_tag="VF097_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003937200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX6261618.1"
                     /translation="ESPYSYLMTRRIERAMALLRRGDLSVTDVCFEVGFSSLGTFSTR
                     FTELVGVPPSVYRQRAGEEMAGIPPCVAKQVTRPIRNREARVAEPRLA"
     gene            276..722
                     /locus_tag="VF097_02095"
     CDS             276..722
                     /locus_tag="VF097_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6261619.1"
                     /translation="MTAMDDITIYQAMLPHGDPNASLAFYRDTLGFEVRGDVEYQGMH
                     WITVGPPGQPGTSIVLYPPQAAPGLTDEERRTIAEMMAKGTFASINLATKDVDAAFER
                     LQAGDAEVVQEPTDQPYGIRDCAFRDPAGNLIRIQELREDEGDRDG"
     gene            715..1167
                     /locus_tag="VF097_02100"
     CDS             715..1167
                     /locus_tag="VF097_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX6261620.1"
                     /translation="MAETKTSRKSTRKKPSKGFTAEERAAMKERAKELKGRGGKEAGR
                     KALFEKIAEMPEPDRSMAQRIHEIVGEAAPDLEPRTWYGMPAYAKDGKVLCFFTPASK
                     FKERYATFGFNAVANLDQGNMWPTSFALTELSAAEEKTIASLVKKAVR"
     gene            complement(1273..1923)
                     /locus_tag="VF097_02105"
     CDS             complement(1273..1923)
                     /locus_tag="VF097_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX6261621.1"
                     /translation="MGAQPSSEGKVMPEILVPTVVEHTSRGERSFDVYSRLLNERIVF
                     LGTPIDDTVANLATAQLLHLDSVDSSKDVNLYINSPGGSATALLAIYDTMQYLRSDVS
                     TICIGQAASAAAVLLACGTKGKRYALKHARVLMHQPSGQVGGQAADIDIHAKEILRQR
                     ELVEEILVRHTGQTREKISADTERDFILTADAAKDYGLVDEVIESRAGLTPVATDK"
     gene            1977..3185
                     /locus_tag="VF097_02110"
     CDS             1977..3185
                     /locus_tag="VF097_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261622.1"
                     /translation="MSDHPTESSLTSVPVPAAAPIAVLGALLHQVAVYVPVLRFRAGP
                     DSFTESIVSFTDGFRAALPHLAVTVLGLAGAFLILRRRELAAGLFFAGGTMSLVTFAI
                     AIRGAWWAASAGSLPIVADVGLYLGLAGAALLLAAGVLSLRGLLGGGRLLPAVALASF
                     FLGAWLIPWLAWRAVEQPPPGLDVGAMGWFGRALIATTFAALALAVALMSWTLSPTRG
                     RGLGTIAGLALVLLVVGIVAGWSLVRILHAGAWGAIGVFLGLVPLAFSRAVAVPTLPG
                     AFGAGSLILLLTGILVPVLATERGGSFRPIPWGDVPVADLLPNAVNELPAIALAALGV
                     ALIRHRDLAAGLLIGAAGAAIPWVLLYSVEGIEVGMWLRIGGLLLAAAAAWALLATSG
                     RPGSVPPSPR"
     gene            complement(3142..4605)
                     /gene="gcvPB"
                     /locus_tag="VF097_02115"
     CDS             complement(3142..4605)
                     /gene="gcvPB"
                     /locus_tag="VF097_02115"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX6261623.1"
                     /translation="MSGAPVRTGDPPTAGLLRDLSRPGRRASTFPALDVPEAELPAEH
                     RRDQPPPLPEVAERDLVSHFTRLSQRNYGVDTGFYPLGSCSMKYNPKIADTVAALPGF
                     QRLHPHQPEATIQGALEMLWRLERALCEVTGMARATFQPAAGASGELTGLLIMRAFHQ
                     RNGDARRRIVIPDSAHGTNPASVTLAGYEVVEVPSDARGLVDVSALEKLVDEDVAGLM
                     ITNPNTLGLFEEGILDIARVIHDAGGLLYYDGANLNAILGRVRPGDMGFDIVHMNTHK
                     TFATPHGGGGPGAGPVAVMERLAPFLPAPVVARDDDGAFRWDHDRPDSIGRVHGFHGN
                     VGVLVRAYAYVFAHGGDGLREVGERAVLNARYLAELVKGGFPLAYDQPPMHEFVSTAK
                     PLKRTGVRAMDVAKRLIDLGFHPSTVYFPLIVEEAMMVEPTETESKETLDAFAEAMLR
                     AAREAREDPELLHRAPTTTPVGRLNEAKAARSLDVRW"
     gene            complement(4602..5915)
                     /gene="gcvPA"
                     /locus_tag="VF097_02120"
     CDS             complement(4602..5915)
                     /gene="gcvPA"
                     /locus_tag="VF097_02120"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006446666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX6261624.1"
                     /translation="MRFAPHTDTDVREMLEAIGLSSVDDLYAHIPAEVLLDRPLALPQ
                     SVSEMELLGDLRELASRNHHADDLVCFAGGGAYDHYVPAVVWALAARSELQTSYTPYQ
                     PELSQGVLQALYEYQSMICDLTALEVSNASLYDGPTALVEAVNLARAATGRERVLVAG
                     TVDERYVETLRTYGRGAGYVPEVLPGDGVSPVPEVGGDAACVVLQHPNANGGLEPARE
                     VFEAARSAGARAIQVFDPLSLGVLAPPGELGADVAVAEGQSLGNHLSLGGPYLGVIAA
                     RLEDVRRLPGRIAGRTEDEEGNLGFVLTLQAREQHIRRAKATSNICTNQTLMAIAAAI
                     YLGWLGPQGLRELGEASAAKARHAAGLLAEIDGVGLAYPEVPFFKEFALRFGSREPSS
                     VRDALLGHGYLVGPVSGDALLVACTERRTREEIEGLARAMKEVLG"
     gene            complement(5925..6296)
                     /locus_tag="VF097_02125"
     CDS             complement(5925..6296)
                     /locus_tag="VF097_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261625.1"
                     /translation="MRGITLDGDQALKSETVDFAHASERQFAQLLDFYQIEWEYEPRS
                     FDLEFDREGNAVQRFTPDFYLPQYDLFIEITTMNQKLVTRKNRKVRRLRELYPHVNCK
                     IFYQRDYLSLVTKYGLEDRAG"
     gene            complement(6293..>6475)
                     /locus_tag="VF097_02130"
     CDS             complement(6293..>6475)
                     /locus_tag="VF097_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261626.1"
                     /translation="HLGEAPQEMTLLSDGRNIYAAKSRDEIIDLMQQGQGTMFTLQVD
                     RVWTDLEKTLGRKTGS"
BASE COUNT          880 a         2337 c         2256 g         1002 t
ORIGIN      
        1 gagtcgccct acagctacct gatgacgcgt cggatcgagc gcgccatggc gctgctgcgc
       61 cgtggggatc tgagcgtcac cgacgtgtgc ttcgaggtgg ggttctcgtc gctggggacc
      121 ttcagcaccc gtttcaccga gctggtcggc gtgccgccca gcgtctaccg gcagcgggcg
      181 ggggaggaga tggcggggat cccgccgtgc gtggccaagc aggtgaccag accgatcagg
      241 aatcgagaag cgcgcgtcgc cgagccgcgg ctagcatgac cgccatggac gacatcacga
      301 tctaccaggc catgctgccg cacggcgatc cgaacgcctc cctggccttc taccgggaca
      361 ccctcggctt cgaggtccgg ggagacgtcg agtatcaggg gatgcactgg atcacggtcg
      421 gcccccctgg ccagcccggc acgtcgatcg tcctgtaccc gccgcaggcg gcccccggcc
      481 tcacggacga ggagcgacgg accatcgccg agatgatggc caagggcacc ttcgccagca
      541 tcaacctggc cacgaaggac gtcgacgcgg ccttcgagcg gctccaggcc ggcgacgccg
      601 aggtcgtcca ggagcccacc gaccagccgt acgggatccg ggactgcgcg ttccgcgatc
      661 ccgccggcaa cctgatccgc atccaggagc tgcgagagga cgagggagac cgagatggct
      721 gagacgaaga cgtcgcggaa gagcacgagg aagaagccct ccaaggggtt cacggccgag
      781 gaacgagccg ccatgaagga gcgcgccaag gagctgaagg ggcggggcgg caaggaggcc
      841 ggaaggaagg ccctcttcga gaagatcgcc gagatgccgg agccggaccg gtccatggcc
      901 cagcggatcc acgagatcgt cggggaggcc gccccggacc tcgagccgag gacgtggtac
      961 gggatgcccg cctacgccaa ggacggcaag gtgctctgct tcttcacgcc cgcgtcgaag
     1021 ttcaaggagc ggtacgcgac gttcggcttc aacgccgtgg cgaacctgga ccagggcaac
     1081 atgtggccca cctccttcgc gctgacggag ctgtccgcgg ccgaggagaa gacgatcgcc
     1141 tccctcgtga agaaggcggt gcgctgaggc tcgacctcgg cctccctccg cccgcttcgc
     1201 ccataacccg cattatgaca agtaaccttg agcgggaccc tcccgctcgc ctctcccgct
     1261 cgtcgggggg ccctacttgt cggtggccac gggggtcagg ccggcccggg actcgatcac
     1321 ctcgtccacg aggccgtagt ccttggccgc gtcggccgtc aggatgaagt cccgctcggt
     1381 gtcggcggag atcttctccc tcgtttgccc cgtgtggcgg acgaggatct cctccaccag
     1441 ctcgcgctgg cgcaggatct ccttggcgtg gatgtcgata tcggcggcct gtccgcccac
     1501 ctgcccgctg ggctggtgca tcagcacccg ggcgtgcttc agcgcgtagc gcttcccctt
     1561 cgtgccgcag gccagcagca ccgcggcagc ggaggcggct tggccgatgc agatcgtgct
     1621 cacgtcggac cggaggtact gcatcgtgtc gtagatcgcc agcagggccg tggccgaccc
     1681 ccccggcgag ttgatgtaca ggttgacatc tttgctggaa tcgaccgaat cgaggtggag
     1741 gagctgggcc gtggccaggt tggccacggt gtcgtcgatc ggggtgccca ggaagacgat
     1801 gcgctcgttc aggagccgcg agtagacgtc gaaggagcgc tcgcccctcg aggtgtgctc
     1861 cacgacggtg gggaccagga tctccggcat gaccttccct tcgctgctcg gctgcgcccc
     1921 catggtaccc tccccctccc ccaacggcgc gtccctcgtc ggtaaactcg ggccaggtga
     1981 gcgaccaccc caccgagtcg agtctcacga gcgtccccgt cccggcggcc gcgcccatcg
     2041 cggtcctcgg cgccctgctc caccaggtcg ccgtctacgt gcccgtcctc cggttccggg
     2101 cgggtcccga ctccttcacg gagtcgatcg tctcgttcac cgacggcttc cgggcggccc
     2161 tgccccacct cgccgtgacc gtcctcggcc tcgcgggcgc ctttctgatc cttcggcggc
     2221 gagagcttgc cgccggcctg ttcttcgccg gcggcacgat gtccctcgtc accttcgcca
     2281 tcgccatccg cggggcctgg tgggccgcct cggccggctc gctcccgatc gtggcggacg
     2341 tcggcctgta cctgggcctg gccggcgcgg ccctgctcct cgcggccggc gtcctctccc
     2401 tgcggggcct cctgggcggc gggcggctcc tgcctgcggt ggcgctggcg tcgttcttcc
     2461 tcggggcctg gctgatcccc tggctcgcct ggagggccgt ggagcagccc ccgccggggc
     2521 tggacgtggg cgccatgggg tggttcgggc gggccctgat cgccacgacc ttcgccgcgc
     2581 tcgccctcgc cgtcgccctc atgagctgga cgctcagccc cacccggggc cgcggcctgg
     2641 ggacgatcgc ggggttggcg ctcgtcctgc tcgtcgtggg catcgtagcc ggctggagcc
     2701 tggtcaggat cctccacgcc ggggcgtggg gagcgatagg cgtcttcctg ggcctcgttc
     2761 cgttggcctt ctcccgggct gtcgccgtgc cgaccctccc cggagccttc ggcgccggaa
     2821 gcctgatcct gctcctgacg gggatcctcg tccccgtcct ggcgaccgag cgcggtggga
     2881 gcttccgccc aatcccctgg ggcgacgtgc cggtggccga cctcctcccc aacgccgtga
     2941 acgagctccc ggccatcgcg ctggcggcgc tcggagtggc gctgatccgg caccgagatc
     3001 tggccgccgg gctcctgatc ggggccgcgg gggcggcgat cccctgggtg ctcctctact
     3061 ccgtagaggg gatcgaggtc gggatgtggc tgcggatcgg cgggctgctg ctcgccgccg
     3121 ccgcggcctg ggccctgctg gctaccagcg gacgtccagg ctccgtgccg ccttcgcctc
     3181 gttgagccgc cccacagggg tcgtggtcgg cgcccggtgg agcagctcgg gatcttcccg
     3241 ggcctccctc gccgcccgaa gcatcgcctc ggcgaacgcg tcgagcgtct ccttcgattc
     3301 ggtctcggtg ggctcgacca tcatcgcctc ctccacgatc agcgggaagt agacggtcga
     3361 ggggtggaag cccaggtcga tgagccgctt cgccacgtcc atcgcccgaa cccccgtcct
     3421 cttgagcggc ttcgcggtcg agacgaactc gtgcatcggc ggctgatcgt aggcgagggg
     3481 gaagcccccc ttcacgagct cggccaggta ccgggcgttg aggacggccc gctcccccac
     3541 ctcccgcagc ccgtcgcctc cgtgggcgaa gacgtaggcg tacgcgcgca cgagcacccc
     3601 gacgttcccg tggaagccgt gcacacgacc gatcgagtcc ggacggtcgt ggtcccagcg
     3661 gaaggcgccg tcgtcgtccc gggccacgac cggggccggc aggaacggcg ccagccgctc
     3721 catcaccgcc accgggcccg cgcccggccc tcccccaccg tggggcgtgg cgaaagtctt
     3781 gtgcgtgttc atgtgcacga tgtcgaagcc catgtcgccc ggccgcaccc tgcccaggat
     3841 cgcgttgagg ttggcgccgt cgtagtacag gagtccgccc gcgtcgtgga tcacgcgggc
     3901 gatgtccagg atcccctcct cgaacagccc gagcgtgttg gggttggtga tcatcagccc
     3961 ggccacgtcc tcgtccacaa gcttctccag ggccgagacg tcgacgagcc cccgcgcgtc
     4021 ggaggggacc tccaccacct cgtacccggc cagcgtgacg ctcgcggggt tcgtgccgtg
     4081 ggccgagtcc gggatcacga tgcggcggcg ggcgtcgccg ttccgctggt ggaaggcccg
     4141 catgatgagg aggcccgtca gctcgccgct ggcgccggcg gccggctgga aggtggcccg
     4201 ggccatcccc gtgacctcgc acagcgcccg ctccaggcgc cagagcatct cgagcgcccc
     4261 ctggatcgtg gcctcgggct ggtggggatg gagtcgctgg aagcccggga gggccgccac
     4321 cgtgtcggcg atcttggggt tgtacttcat cgaacacgac cccagcgggt agaagcccgt
     4381 gtccaccccg tagttccgct ggctgagccg cgtgaagtgg ctcacgaggt cgcgctcggc
     4441 cacttcgggc agcggcggag gctgatcccg ccggtgctcg gcggggagct cggcctcggg
     4501 aacgtcgagc gcgggaaacg tcgaggcccg tcgtccaggc cgcgagaggt cccggaggag
     4561 ccccgcggtg ggcgggtccc ccgtccggac cggcgccccg ctcaccccag cacctccttc
     4621 atggcccggg ccagcccctc gatctcctcc cgcgtcctcc gctcggtgca ggccacgagc
     4681 agcgcgtcgc cggagaccgg gcccacgagg tacccgtggc ccaggagggc gtcccgcacg
     4741 gaggacggct cccgggaccc gaaccgcagc gcgaactcct tgaagaacgg cacctcggga
     4801 taggccaggc ccaccccgtc gatctcggcg agcaggccgg ccgcgtggcg agccttggcc
     4861 gccgaggcct cccccagctc ccggagcccc tgcggcccca gccagccgag gtagatcgcg
     4921 gccgcgatcg ccatcagcgt ctggttcgtg cagatgttcg acgtggcctt ggcccggcgg
     4981 atgtgctgct cccgggcctg aagggtgagc acgaagccga ggttcccttc ctcgtcctcg
     5041 gtccggccag cgatgcgccc cggcaggcgg cgcacgtcct cgagccgggc cgcgatcacc
     5101 ccgaggtaag gcccgcccag gctgagatgg ttgccgagcg actggccctc ggccacggcc
     5161 acgtcggccc ccagctcgcc gggcggcgcc agcacgccga gcgacagcgg atcgaagacc
     5221 tggatcgccc gcgcccccgc cgaccgggcc gcctcgaaga cctcccgggc cggctcgagg
     5281 ccgccgttgg cgttggggtg ctggaggacg acgcaggccg cgtcgccgcc cacctcgggc
     5341 accgggctga ccccgtcccc ggggaggacc tcgggcacgt accccgctcc cctgccgtag
     5401 gtccggaggg tctccacgta ccgctcgtcc accgtccccg ccacgaggac ccgctcgcgg
     5461 ccggtcgccg cccgggccag gttcaccgcc tccacgagcg ccgtcggccc gtcgtacagc
     5521 gacgcgttgg agacctccag cgcggtcagg tcgcagatca tcgactggta ctcgtacagc
     5581 gcctgcagga caccctgtga cagctcgggc tggtagggcg tgtacgaggt ctggagctcc
     5641 gagcgggccg ccagcgccca cacgacggcg ggcacgtagt ggtcgtaggc ccccccgccc
     5701 gcgaagcaga cgaggtcgtc ggcgtggtgg ttcctgctcg ccagctcgcg cagatcgccg
     5761 agcagctcca tctccgagac gctctgcggg agggcgagag ggcggtccag cagcacctcg
     5821 gcggggatgt gcgcgtagag gtcgtccaca gaggagagcc cgatggcctc cagcatctcg
     5881 cggacgtccg tgtcggtgtg gggggcgaag cgcacggggg aatcctaccc cgcgcgatcc
     5941 tcgagcccgt acttggtcac gagcgacagg tagtcgcgct ggtagaagat cttgcagttg
     6001 acgtgcgggt acagctcccg cagcctgcgg accttgcgat tcttccgcgt gaccagcttc
     6061 tggttcatcg tcgtgatctc gatgaagagg tcgtactgcg ggaggtagaa gtcgggggtg
     6121 aagcgctgga cggcgttgcc ctcacggtcg aactccaggt cgaaggagcg cggctcgtac
     6181 tcccactcga tctggtagaa gtcgagcagc tgcgcgaact ggcgctcgct ggcgtgggcg
     6241 aagtcgaccg tctcgctctt gagggcctgg tccccgtcga gcgtgatgcc cctcaactcc
     6301 cggtcttcct ccccagcgtc ttctcgaggt cggtccacac ccggtccacc tggagggtga
     6361 acatcgtccc ctgaccctgc tgcatcaggt cgatgatctc gtccctcgac ttggcggcgt
     6421 agatgttccg gccgtcggag aggagggtca tctcctgggg cgcctcgccg aggtg
//
LOCUS       DASUTZ010000047         4701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_919772, whole genome shotgun sequence.
ACCESSION   DASUTZ010000047 DASUTZ010000000
VERSION     DASUTZ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919772"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..2586)
                     /locus_tag="VF097_02135"
     CDS             complement(34..2586)
                     /locus_tag="VF097_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEX6261627.1"
                     /translation="MSQASPHGETAVRAGLSTEEARRRLEEHGPNELPEPEHEGPAAR
                     LLRQLREPMAILLLVAAGVAGIGLGERLDGIAIVAIVILNAVIGFVTEGRAQRALEAL
                     RSMETPRARVRRSGRVTTVPAREVVPGDVVLLSPGDRVPADLRLLDASGLEIDESMLT
                     GESLPVAKEPGAEEAPDAGLGERPGSAHSGTLVSRGVGEGEVVATGADTALGEIGAQL
                     GGPEQRTPLQDDLARLTKILGLVAVVVAAGVMALTLIRTGLSGESIQEAFLAAVALAV
                     AAVPEGLATVVAIGLALGVRRMAEHGAIVRRLPAVETLGSTTVILTDKTGTLTQNRMR
                     LAVVAAGDEEVRAVDDLPSVVADRVRRVATLCSDAELDPPEGDPIEVALLEAVGADEV
                     ERLREQHPRISQLAFDAERRRMTTAHRAGDGVLLLMKGAPEVVIERSTRLLEADGEEV
                     ALGDERRRRLVERAESLAGRGMRLLALADRGPMDLPDDLEDAERDLTLVGLAGLADPV
                     RDEASDAVGEARQAGIDVLMVTGDHAGTAASVAREVDLRESGVVTGADLRRDGLPEDP
                     RAVSVYARVDPGEKLRLVDALQGQGEIVAVTGDGVNDAPALQRAHIGVAMGRGSDVAR
                     ESSDMVLTDDNLATIVRAVRQGRGIYDNIRKVVDYLIGGNLSEIAVVVGAFLLFPALG
                     TPLLPLQLLWINLLTDGLPALALGADAPDPGLMRHPPRDPSAGILPLRKIGVLSVRGL
                     LIATASIAGLALARFAWDGSWDRARGLMFTVLVIAHLWYAFVVRGRGVGLFSNPWLVS
                     AVLSGIALQIGVVTWEPARDLFGIEALEARDWAAAAVLGVLPALAMMPFRRP"
     gene            complement(2583..3179)
                     /locus_tag="VF097_02140"
     CDS             complement(2583..3179)
                     /locus_tag="VF097_02140"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6261628.1"
                     /translation="MHVKDVMTTPVVTLRPETPIKEAAALLVRHGVSGAPVVDEHEQL
                     VGIVSESDLMALETTPDPRSRIRLPRWRRVRVPRRVEQVMTRDVVTLPERADVADAAR
                     LMLERRVKRIPVVAEGRVVGIVSRRDVLRLLARTDGEIRAELQELLSEEILILGRFEA
                     QVEDGVVTLAGPPDPRARRLAELLARSVGGVVGVRFTT"
     gene            3345..3755
                     /locus_tag="VF097_02145"
     CDS             3345..3755
                     /locus_tag="VF097_02145"
                     /inference="COORDINATES: protein motif:HMM:NF025066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEX6261629.1"
                     /translation="MTESLSLVLFSGTDDRLTAAATMATGAAAMGRRVDVFLQFWALD
                     AFRADRIEKDHGVVHEAGPEGAAALRRMGEKGGAHWSELFRQAKDIGELEITACAHSM
                     EMLELERDDLDPLVTDVIGIAAYMARVDGPVVFI"
     gene            3768..4007
                     /locus_tag="VF097_02150"
     CDS             3768..4007
                     /locus_tag="VF097_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012966902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX6261630.1"
                     /translation="MEATKTQETIDARGMPCPGPLMSLIGSIRQAEVGAVIEVLSSDE
                     GSLTDIPAWVEKAKQEMLEVASEEGYTRFVVRKAR"
     gene            4007..>4701
                     /locus_tag="VF097_02155"
     CDS             4007..>4701
                     /locus_tag="VF097_02155"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6261631.1"
                     /translation="MPARIVVLGGGVGGTLAANLLSKELGRDADVKVVDPTGMHVYQP
                     GFLYVALGQAHGRWLARDERTLLRKDVELVVEAATRVDPEAGTVELERGGTLPFDYLV
                     VATGARLMKDEIPGLIEGSHDFYSLDGALRLREALLAFPGGRILVGVAGIPYKCPPAP
                     VEFVLMLEETLRKRGVRDRSEVTLLSPLNRAFTIESASKLVQPIMERRGIGLETFFNV
                     EAVDPAAHVVESL"
BASE COUNT          656 a         1720 c         1726 g          599 t
ORIGIN      
        1 tcggtcccgc gccatgcggc ccgagcggcc ccctcagggt cggcggaagg gcatcatggc
       61 gagggccggc agcaccccga gcacggccgc ggcggcccag tcgcgggcct ccagcgcctc
      121 gatcccgaag aggtccctgg ccggctccca ggtcaccacc ccgatctgca gggcgatgcc
      181 cgagaggacc gcgctcacga gccaggggtt cgagaacaga cccacgcccc gcccccggac
      241 cacgaacgcg taccagaggt gggcgatcac gaggaccgtg aacatcaggc cccgggcccg
      301 gtcccacgag ccgtcccagg cgaagcgggc cagggccagg ccggcgatcg acgcggtggc
      361 gatcagcagc ccccggaccg agaggacgcc gatcttccgg agcgggagga tgcccgcgct
      421 gggatctcgc ggcgggtggc gcatcaggcc ggggtcggga gcgtcggcgc ccagtgcgag
      481 ggccgggagc ccatccgtga ggaggttgat ccacaagagc tggagcggga ggagcggggt
      541 gcccagcgcc gggaagagca ggaacgcgcc gacgaccacg gcgatctcgg acaggtttcc
      601 tccgatcagg tagtccacga ccttccggat gttgtcgtag atgccgcggc cctggcgcac
      661 ggcccgcacg atcgtggcca ggttgtcgtc ggtgagcacc atgtccgagg actcgcgggc
      721 cacgtcgctg ccccgaccca tcgccacccc gatgtgggcc cgctgcaggg cgggcgcgtc
      781 gttcacgccg tccccggtca cggcgacgat ctcgccctgc ccctggaggg catcgaccag
      841 ccggagcttc tcgccggggt ccaccctggc gtagacagag acggcccggg ggtcctcggg
      901 gaggccgtcg cggcgcaggt cggcgccggt gaccacaccg ctctcccgga ggtccacctc
      961 cctggccacc gaggccgcgg tcccggcgtg gtcgccggtc accatcagga cgtcgatgcc
     1021 ggcttggcgg gcctcgccga cggcgtccga ggcctcgtct cggacggggt cggcgaggcc
     1081 cgccagcccc accagcgtga ggtcgcgctc cgcgtcctcc agatcgtccg gaaggtccat
     1141 cggtcccctg tccgcaagcg ccagcagccg catccccctc cccgcgaggc tctcggcccg
     1201 ctctacgagg cgccgccgcc gctcgtcccc cagcgcgacc tcctcgccgt ccgcctccag
     1261 caggcgggtc gaccgctcga tcacgacctc gggcgcgccc ttcatcagga gcaggacccc
     1321 gtcccccgct cggtgcgccg tcgtcatccg gcgacgctcc gcgtcgaagg cgagctgcga
     1381 gatcctcggg tgctgctcgc gcagccgctc gacctcgtcc gcgcccacgg cctcgagcag
     1441 cgccacctcg atcggatcgc cctcgggcgg gtcgagctcc gcgtcgctgc agagggtggc
     1501 cacgcgccgc acgcggtcgg ccacgacgga ggggaggtcg tccacggccc ggacctcctc
     1561 gtccccggcg gccaccacgg cgagacgcat ccgattctgg gtcagggtcc ccgtcttgtc
     1621 ggtgaggatc acggtcgtgg acccgagcgt ctccaccgcc ggaagccggc gcacgatcgc
     1681 cccgtgctcg gccatccggc gcacgccgag ggccagcccg atcgccacga cggtggccag
     1741 gccctcgggg acggcggcca cggccagggc cacggccgcc aggaaggcct cctggatcga
     1801 ctcgccggag agcccggtgc ggatgagggt gagcgccatc acccccgccg ccacgaccac
     1861 ggcgacgagc cccaggatct tcgtcagacg ggccaggtcg tcctggagcg gggtccgctg
     1921 ctccggcccg cccagctggg cgccgatctc gcccagcgcg gtgtcggcgc cggtggccac
     1981 gacctcgccc tcgcccaccc ctcggctgac gagggttccc gaatgagcgg acccgggccg
     2041 ctccccgagg cccgcgtcgg gggcctcctc ggcccccggc tccttggcca cgggcagcga
     2101 ctccccggtg agcatggact cgtcgatctc caggccggac gcgtcgagga gccgcaggtc
     2161 ggcggggacg cggtcgcctg gcgagaggag cacgacgtcg ccggggacca cctcgcgggc
     2221 gggcacggtc gtgacccgtc ccgagcgtcg gacgcgggcg cggggcgtct ccatcgagcg
     2281 gagggcctcg agcgcgcgct gggcgcggcc ctcggtgacg aagccgatga ccgcgttgag
     2341 gatcacgatc gccacgatgg cgatgccgtc gaggcgctcg cccagaccga tcccggccac
     2401 gccggcggcg acgagcagaa ggatcgccat cggctcccgg agctggcgga ggaggcgcgc
     2461 cgccggaccc tcgtgctcgg gctcgggaag ctcgttcggc ccgtgctcct ccagccggcg
     2521 gcgcgcctcc tccgtgctca ggcccgcgcg gaccgccgtc tccccgtggg gggacgcctg
     2581 gctcatgtcg tgaaccgtac ccccaccacg cccccgacgc tcctggccag gagctcggcc
     2641 aggcgacggg cccgggggtc gggcggcccg gccagtgtga ccacgccgtc ctccacctgg
     2701 gcctcgaacc ggcccaggat caggatctcc tcgctcagca gctcctggag ctcggcccgg
     2761 atctcgccgt cggtgcgggc cagcaggcgg agcacgtccc gtcggctgac gatgcccacg
     2821 acgcgaccct ccgcgacgac cgggatgcgc ttgacgcgcc gctccagcat gagcctcgcg
     2881 gcgtcggcca cgtcggcgcg ctcgggcagg gtcacgacgt cgcgggtcat cacctgctcg
     2941 acgcgccggg gcacgcggac ccggcgccag cgggggagcc ggatgcggct cctcggatcg
     3001 ggggtcgtct ccagcgccat caggtccgac tcggagacga tccccacgag ctgctcgtgc
     3061 tcgtccacga ccggggcgcc gctcacgccg tggcggacga ggagggccgc agcctccttg
     3121 atcggggtct cggggcgcag cgtgacgacc ggtgtcgtca tcacgtcctt gacgtgcatc
     3181 gcgccacctc ctggctccac cgtagcgagg ccccctcccg gggagcaggg ccgaaggtcc
     3241 ttcccgcgga ggggcggggg cccaaaggga gggggccggc ggaccctacg acgtggccgg
     3301 gccgcctggg acgcttggga ccacacgcac gagaggagct cgagatgacg gaatcgctct
     3361 ccctggtcct cttctctggg accgacgaca ggctgacggc ggccgcaacg atggccaccg
     3421 gcgccgcggc gatgggcaga cgggtggacg tcttcctcca gttctgggcc ctcgacgcct
     3481 tccgcgcgga ccgcatcgag aaggaccacg gggtcgtcca cgaggccggg cccgaggggg
     3541 ccgcggcgct gcggcgcatg ggcgagaagg gcggggcgca ctggagcgag ctcttccgtc
     3601 aggcgaagga catcggcgag ctcgagatca ccgcctgtgc ccactcgatg gagatgctcg
     3661 agctcgagcg agacgacctc gacccgctgg tgaccgacgt gatcgggatc gcagcgtaca
     3721 tggcccgcgt ggacgggccg gtggtcttca tctgagagga gcgaacgatg gaagcgacga
     3781 agacgcaaga gacgatcgac gcccgaggga tgccgtgccc cggaccgctg atgagcctga
     3841 tcggctcgat ccgccaggcc gaggtgggcg cggtgatcga ggtgctgtcg tccgacgagg
     3901 ggtcgctgac cgacatcccc gcctgggtcg agaaggccaa gcaggagatg ctggaggtcg
     3961 cctccgagga aggctacacg cgcttcgtgg tccggaaggc gcgctgatgc ccgccaggat
     4021 cgtcgtgctg ggaggggggg tcggggggac cctcgccgcg aacctcctgt cgaaggagct
     4081 ggggagggac gccgacgtca aggtcgtgga ccccaccggg atgcacgtgt accagccggg
     4141 gttcctctac gtggcgctgg gccaggccca cgggcgctgg ctggcccgcg acgagcggac
     4201 gctgctgcgg aaggacgtcg agctggtggt ggaagcggcg acgcgcgtgg atccagaggc
     4261 ggggacggtg gagctggagc gaggcggcac gctcccattc gactacctcg tggtggccac
     4321 cggggcccgc ctcatgaagg acgagatccc ggggctgatc gagggatccc acgacttcta
     4381 ctccctggac ggagctctcc ggctccgcga ggccctcctc gcctttccag ggggccggat
     4441 cctcgtgggc gtcgccggga tcccctacaa gtgccccccg gcgcccgtgg agttcgtcct
     4501 gatgctggag gagaccctgc ggaagcgcgg cgtgcgcgac cgctcggagg tcaccctgct
     4561 ctcgccgctc aaccgggcgt tcacgatcga gtcggcctcg aagctcgtcc agccgatcat
     4621 ggagcgtcgc ggcatcggct tggagacctt cttcaacgtc gaggccgtgg acccggcggc
     4681 ccacgtggtg gagtcgctgg a
//
LOCUS       DASUTZ010000048        31537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_530669, whole genome shotgun sequence.
ACCESSION   DASUTZ010000048 DASUTZ010000000
VERSION     DASUTZ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 31537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31537
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530669"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..154)
                     /locus_tag="VF097_02160"
     CDS             complement(<1..154)
                     /locus_tag="VF097_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011904634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX6261632.1"
                     /translation="MTERVTRPRRSCLAVPGSSPKMLAKAASLPADQVFLDLEDAVAP
                     DAKEESR"
     gene            complement(221..952)
                     /locus_tag="VF097_02165"
     CDS             complement(221..952)
                     /locus_tag="VF097_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261633.1"
                     /translation="MDDDRLFEIPDDPDPPDGGHRGTERDLDRRLATIPAELSDVFGL
                     RIRAALDAVQDRTAGSIEGLRSAVAGVEERVTAHLTVIAELLEQSPLAVRDAVRNAAA
                     SIRGDLEVVQTLPQHVARALKVMQEAVAEIAGTEEAVGRQLEALNGAVERIRGDVAAL
                     AARVDQRITRLDALSGVLESLAQKRGFKDLIRSERKAVEQQERFVRDLTDLGGKLSTQ
                     LTELSVRVEHLAERIGRVESQPPRA"
     gene            1069..3531
                     /locus_tag="VF097_02170"
     CDS             1069..3531
                     /locus_tag="VF097_02170"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX6261634.1"
                     /translation="MTRPSIRILIVALLAAGFALGLLPPAGGSGPPETMLSTVPLHEA
                     ELAGRGLLASGQALSATRTSRTPRHEVCADFAFTAVGLTWRQTGDELVPVSFRWDEGS
                     GVRGVAVYADPDHAPDPGTGERARGGTPLVWTGEARCVELTLELQAGVEVRDLRAVFV
                     NTSGTSREGSILGTIATGIGRGMALLWGMETSEAVAPKPKIIRRSGWGANESLRNCGP
                     SYAPELKMAYVHHTASGNGYSKAQADDVIRAIYAYHTQGRGYCDIAYNFLVDRFGRIY
                     EGRFGGMTEPVIGGHAMGFNTGSTGVAVVGNFVSVNPGTAAISALKRLLAWRLDIAHL
                     NPKGTATMVSAGGPNQKYEEGEEVKLPVIAGHRHTGYTTCPGDRLFAKLGAIRTGAQA
                     RGLPKIWNPRATSDTLELGHGGVRYRAKLSAALPWSVEIAGPSGIVRTWTGTGQGVDV
                     VWEGFDDRGFPADEADYIVTMRAGGAGQPQARPAALRTALTGSCDITTTRSSPTAIGT
                     DEDEVLCGLNGQADVLDGGGGNDILMGFGGNDQLTGGPGQDTLVGGNGSDMLQGGTGA
                     DVLFGGAANDTLVGGANADVLHGGPGDDQLDGGGGADTVSHATSSTAVRVDLAAGTAI
                     GEGTDDLTGFVNAIGSPRNDTLIGSDGHNHLVGGGGRDVLTGGAGDDLLEAGPGADRL
                     AGGAGDDALVGGGGSDTARFAGAPGPVVVDLRAGSAIGDGADTLRAIQNVVGGPGKDT
                     LRGDAKANRLLGKGGWDILVGKGGPDLLVGGAGRDDLRGGSGRDTLRAKDGKRDSVDG
                     GGGFDAAHVDPKDDVRRVEQHR"
     gene            complement(3533..4855)
                     /gene="gabT"
                     /locus_tag="VF097_02175"
     CDS             complement(3533..4855)
                     /gene="gabT"
                     /locus_tag="VF097_02175"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEX6261635.1"
                     /translation="MGTINLVTEIPGPRSRELIERKEQVVAEPFDLHMPGFIEEGHGA
                     SFTDVDGNTFLDFAGGLGALLTGHSHPKVVEAARAQLERFAHTDFTVVPYESWVELAE
                     RLVAAVGGGDRKAAFFNSGAEAVENAVKIARRATGRSAIIAFEGAFHGRTLMAMTLTS
                     RHAPYKSGFGPFAPEVYRVPFPYAYRSPEPENAGALALEAFERSLTTQVDPDTVAAVI
                     IEPVLGEGGFVPVTPDFLPGLAEICRRHDILLIADEVQTGCGRTGTFLAVEQFGVEPD
                     LITLAKAMGSGYPISAVVGRADVMDAPGPSEIGGTYIGNPVACAAANATLQVIEEEEL
                     VERAQVVGKTIRAAWEEIARDVPEIGDIRGIGAMIGVEMVKDPETKEPDKELVGGVIG
                     EAMQRGLLAVNCGIYKNVLRHLLPLVISDEELDEGLEVLAESVTAARR"
     gene            complement(4899..5345)
                     /locus_tag="VF097_02180"
     CDS             complement(4899..5345)
                     /locus_tag="VF097_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6261636.1"
                     /translation="MKVHELGHLVLYVRDLERSVRFYRDVLGWEEIGRFGSQGAAFSS
                     GRTHHELLLLEVGEDATPVPPGRRVGMYHFGLKVGTTDDELREAIRALEAAGVQIMGM
                     ADHVVTHSVYIADPDGNEIELYIDVQPERWREDPAAIMSPPRPLRL"
     gene            5445..6452
                     /locus_tag="VF097_02185"
     CDS             5445..6452
                     /locus_tag="VF097_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007976273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6261637.1"
                     /translation="MTKLIEVESLTKVYPDGTRAVDDVSFHVEEGEMFGFLGPNGAGK
                     TTTIRILVTLLPKTAGSARVGGIEVSEDPDTVRRSIGYAAQFIGVDDDLTAHENLVLQ
                     ARLHGIGKAEARRRAEELLEVFALSPVAGRRAATLSGGMRRRLDLAQALAHRPPLVFL
                     DEPTTGLDPQTRNAMWRYLEELNRGGTTVFLTTQYLEEADRLCTRLAIIDHGRIVVEG
                     SPAELKKQVGGDVVTLSLEDHEPETVRRALEAVTAFPGAGEPRVFDHSISLPVKDAGE
                     ALSALVRRLDAAGIPVSRLSMSSPTLDEVFLRHTGERMRVEDPNTRASSPAFRVMRGR
                     R"
     gene            6452..7228
                     /locus_tag="VF097_02190"
     CDS             6452..7228
                     /locus_tag="VF097_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6261638.1"
                     /translation="MRTVAESAILFRRNITKLVRVPMMIFFSLFQPLLWLILFTQIFQ
                     RLGEFPQFQQQGYDSYLMFFAPSVLTMTVLTSAFQSGMGMVTDLETGMLDKFLISPIR
                     RSSVLLGKVMADGFRMLLQGATVLVIALLMGARIETGIPGASIMLVVAVLFGIVWAGL
                     SNIVALRTRHAEMTMMIGILLTFPLLFLSTAMMPEGLLPGWLETVGRFNPVSYVIDTA
                     RAFMNDGYDWGQLGITLAVIAAVGAFTLTGATRAFRRATT"
     gene            7225..7740
                     /locus_tag="VF097_02195"
     CDS             7225..7740
                     /locus_tag="VF097_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEX6261639.1"
                     /translation="MTTVWTVGHSTHPIETFLELLRGAGIRRLVDVRTAPGSRRHPQF
                     GREALAASLEQAGVGYEWRGEELGGWRKARPDSRHTALRSAGFRGYADHMESESFREA
                     LDGLLDEAEERPTAVMCAEALWWRCHRSMIADAVAARGHEARHLMPDGSIAPHRPRFS
                     RVEGGVPVYDP"
     gene            complement(7737..8111)
                     /locus_tag="VF097_02200"
     CDS             complement(7737..8111)
                     /locus_tag="VF097_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261640.1"
                     /translation="MALGRRRRIPRGFERVAAEVERAKDALVAAVPASRREPEPLATS
                     ILAFEDALAEAARVLGAWEGAPDDLARRCRSAIEEAARRSEWLRLEAPSLDFESLVLV
                     LGDVIAPLDAFGDVEHEVRRRA"
     gene            8164..9075
                     /locus_tag="VF097_02205"
     CDS             8164..9075
                     /locus_tag="VF097_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX6261641.1"
                     /translation="MRLLLVVNTRAGTVTPRTVRFIERALSDQAKVRIELTREPGHGV
                     ELAGKAAAGDHDVVAVMGGDGTVNEVANGLAGSDVPMAIIPGGGTNVLARSLGIPQEP
                     IRAAGFLLARLSGPGRRIPLGRAAERYFTFSCGFGLDAEIVRRVERRQRLKRAAGHGY
                     FVWTALRVGLVSYDFKTPRIRARWGSALEHERDGLTFLISQNVDPYSYLGSLPIKVCP
                     RASLDLGLDAFAGGPVGRIRIVRWALKTLGSGRHARDRRALSLHDQGRIEVVAASPHP
                     VQMDGEYVGDHDRLLLESVPGALSILA"
     gene            complement(9079..9840)
                     /locus_tag="VF097_02210"
     CDS             complement(9079..9840)
                     /locus_tag="VF097_02210"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX6261642.1"
                     /translation="MEPRRPLVIAHRGASADHPENTLTAFAVAAEVGADLVELDARLT
                     ADGAVVVLHDPELERTTDARGLVHEVRLAQVKEADASGGRGPREEVPTLGEVLELLEG
                     TGTGVDVEIKNLPGEPSYRPGGDEIVEAVTEALEGFPGAGLITSFDPPTLRTAVEAAP
                     DVPTGLLVLPGGELLGLDLAHEDGHRWLLPHAGVFDHGGDDLLDAAARLGIEVGTWTV
                     DEEGQMERLFARGLAAVATNRPALAVAARERALGT"
     gene            9989..10246
                     /locus_tag="VF097_02215"
     CDS             9989..10246
                     /locus_tag="VF097_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX6261643.1"
                     /translation="MDWVQRARCRDEDPELFFPVGTSGPALEQIERARAVCMQCEVRE
                     ECLEWALASGQDAGVWGGTSEEERKAMRRTRRLGPRVPVLR"
     gene            10252..10410
                     /locus_tag="VF097_02220"
     CDS             10252..10410
                     /locus_tag="VF097_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261644.1"
                     /translation="MRALPEPNHEPIGFSHEVFPTSRRWLGLALLMILTIATAVAAAV
                     RLVGIADP"
     gene            10445..11059
                     /locus_tag="VF097_02225"
     CDS             10445..11059
                     /locus_tag="VF097_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEX6261645.1"
                     /translation="MNALAQALPMPRARGARLAFQAALAIGGSLLIAALAQVSVRLPF
                     TPVPISGQTLGVLLVGTAFGWLLGGLTLALYLAEIAVGLPFAAEGNAGYEVLTLVTPS
                     GGYIWGFLAAAILMGWLAGRGWDRSIRSSISVMLLGHIVIYVVGIVWLHASPTFAAFL
                     GHEPSVKETLTAGLYPFVVGDVLKLLLAAGALPLAWRLVGRRGD"
     gene            complement(11056..11730)
                     /gene="nth"
                     /locus_tag="VF097_02230"
     CDS             complement(11056..11730)
                     /gene="nth"
                     /locus_tag="VF097_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX6261646.1"
                     /translation="MARALTRESPLAERGPEVIDLLKREYRGARVALAFETPLECLVA
                     TILSAQSTDKKINEVTATLFQKYRTAEDYLKVPDDELKEDLRPTGFFNQKTKAVRGAC
                     RMIVEEFGGEVPDSMDDLVRLPGVARKTANIVLWNAYPEKAKDDPDAGIAVDTHVKRV
                     SGRLGLTSQKDPDKIERDLMALLPRDDWSGFSYMLIEHGRRICDAKRPRCEDCVVSHL
                     CPASRV"
     gene            complement(11730..12521)
                     /locus_tag="VF097_02235"
     CDS             complement(11730..12521)
                     /locus_tag="VF097_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX6261647.1"
                     /translation="MTLVRTQPGRIWRITLARPDQSNAVNAAMLGELMRALGDAAADR
                     DARVVLLSGEGRHFCAGADIDELARATEGPDGIDYGRALEEVMRSIEDLPVPALAVVQ
                     GSALGAGCQLVVACDLGIAAVDATFGIPSGRLGVVVGYENIQRLVLAVGPKRAGALLL
                     GTRTISGQEAADWGLVGEAVPPEDLPDRALFYAEEIADAAPLSVQASKRGIRQVLSRL
                     SIDREAEGFRVADFDMMAAHAFGSEDLQEGIAAFRERRDPDFRGR"
     gene            complement(12553..13176)
                     /locus_tag="VF097_02240"
     CDS             complement(12553..13176)
                     /locus_tag="VF097_02240"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HEX6261648.1"
                     /translation="MRGASRLLAAGVGILVAALLGSTTAAFSTVTENPANTFQAAPSF
                     CGAQTLAAIGDAWVVTNPSGTNHGGEAELSVVSRAQDRRTFVAFDLPPVPAGCALTDA
                     RLRLHATSSTGARTILAYRAASSWTEGGVTWANQPATSGSPASSPSGTGWREWPVLQQ
                     VLEMYASGNHGFMLRDQVEGSGGQGFEQAYDSRESGPDGPALVLTFG"
     gene            complement(13189..13698)
                     /locus_tag="VF097_02245"
     CDS             complement(13189..13698)
                     /locus_tag="VF097_02245"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX6261649.1"
                     /translation="MDATLQARASARPRIPGVSLVVLGTLTILLFVMVTMMVAGFRFL
                     VVRSGSMEPAIGTGDVVVVKTVRPGEVAPGDVVTFRDATREGSLVTHRVVSVERRHGR
                     YDFVTRGDANTGQERWSVGAEGTIGVVRSAIPMVGYPLHWLGSATVRVVLLVGGSILV
                     GGALLRRVW"
     gene            complement(13698..14303)
                     /locus_tag="VF097_02250"
     CDS             complement(13698..14303)
                     /locus_tag="VF097_02250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SipW-dependent-type signal peptide-containing
                     protein"
                     /protein_id="HEX6261650.1"
                     /translation="MTDAHASPAAGRRERRTGAKIGLTMLILALLGAVVGLGTLSAFS
                     ATTENPGNAFDAGTVSITDDDSGTAMLSLANARPGDSDTACIVVTYTGTLPATVRLYG
                     TTGGTGLDQYLDLTVTRGMIAGPSFDSCAGFTPDGGGGLIYSGTMAGYPDAYGAALVD
                     PDGSWTSGESRAYRFEVSVQDDGGAQGLNATQSFTWEARNT"
     gene            complement(14441..15178)
                     /gene="trpA"
                     /locus_tag="VF097_02255"
     CDS             complement(14441..15178)
                     /gene="trpA"
                     /locus_tag="VF097_02255"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX6261651.1"
                     /translation="MSTLAIYLMASRGLPGLVEAAVEAGADLVEVGFPHSDPLADGPV
                     IRRAGERALAEGMGPAACLEALSRARRRVEVPLVPMTYSAVLEAYGWDRFAAEALEAG
                     ASSLIVADLPSDAMPELRRVHLVAPTTPTTRIRLASGRTDGWLYLVSVAGTTGPRGEA
                     PPLLPDLIRRARAVTALPLLAGFGIASPAHAAAAARLADGVVVGSRAVEAVEEGGPEG
                     LRDLVAALRAALDSADSGRGVSVGSQP"
     gene            complement(15175..16287)
                     /gene="trpB"
                     /locus_tag="VF097_02260"
     CDS             complement(15175..16287)
                     /gene="trpB"
                     /locus_tag="VF097_02260"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX6261652.1"
                     /translation="MRVGIGRGFGGRFVPETLIPALDELAEAWEEARADPGFRQELDR
                     LGRTYGGRPTPLFPAERLAPERRLYLKREDLLHTGAHKLNNALGQGLLARRLGKRRVV
                     AETGAGQHGVAAAAVCALLGLDCVVYMGQEDARRQHPNVERMRLLGAEVRLVSFGTRT
                     LREAVSEAIRDWIEHVDDTHYLLGSCVGPEPYPDLVATLQEVIGREAREQILAAEGRL
                     PDVVVACVGGGSNAIGLFRAFVDDPGVRLIGVEAEGAASLTRGRPAVLHGARSYALAD
                     EDGQIHDARSISAGLDYPGVGPEHARLRESGRVEYVTASDEEALEAFRLLSRAEGIIP
                     ALEPAHAIARALELDAQLVVVGLSGRGDKELGTVLG"
     gene            complement(16287..17447)
                     /locus_tag="VF097_02265"
     CDS             complement(16287..17447)
                     /locus_tag="VF097_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261653.1"
                     /translation="MRRFRHALSTPGLSAIAEIKRRSPSAGDLRPDADVPRLARAFER
                     AGAVALSVLVDERFAGSLGDLRAARAATDLPILAKGFFRTEEELEAVRRAGADAALLL
                     LRDLDDRATRRLMARAEEMGLETLVEAHDGDELGRAAGLGADPIGVNARDLSTFRVDR
                     ARQLGLVVRARGGVVVAESGVTSRAQGAEAELAGADAILVGTALMRAPDPAAKLRELI
                     ARPLVKVCGLTRDEDVAAAAEAGADMAGFVLAESPRRVGRPLDVPDEMLSVGVVVGAT
                     RPIGTDLVQAYASEEGHRGRDGLLLRRGRVVARVPDLPWTGDDPAHLGRAARVPGRVM
                     LAGGLTPGNVAAAVRMVRPWAVDASRGLESAPGIKDPGTIRAFVARAREAAS"
     gene            complement(17444..18472)
                     /gene="trpD"
                     /locus_tag="VF097_02270"
     CDS             complement(17444..18472)
                     /gene="trpD"
                     /locus_tag="VF097_02270"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX6261654.1"
                     /translation="MSITDALARLLEGSDLSREEARAVTGEIMRGEATPAQIGGFLVG
                     LRMKGESPDEIAGCAEAMREHVLPVAPRRRDLIDTAGTGGDGAGTLNLSTAAGLVAAG
                     AGAGVAKHGNRAVSSASGSADVLEALGFSLDLSPERIAESIDEHGFGFLFAPAHHPAM
                     RHAGPVRRELAARTVFNVLGPLTNPAGARAQLVGVYARELAGTVARVLALLGADRALV
                     VHGAGGIDELSPAGPNLVFEVEEGRVEETIVDPADLGIDGCAPDDLRGGTPEENAAAI
                     REVFDGAAGPKRDAVLLNAAAALRIAGLVSDLREGLAAAAEVVDGGAAAEKLETLVAF
                     SRAEEAVA"
     gene            complement(18478..18717)
                     /locus_tag="VF097_02275"
                     /pseudo
     CDS             complement(18478..18717)
                     /locus_tag="VF097_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003961131.1"
                     /note="TrpG; with TrpE catalyzes the formation of
                     anthranilate and glutamate from chorismate and glutamine;
                     TrpG provides the glutamine amidotransferase activity;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate/aminodeoxychorismate synthase
                     component II"
     gene            complement(18915..19667)
                     /locus_tag="VF097_02280"
     CDS             complement(18915..19667)
                     /locus_tag="VF097_02280"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEX6261655.1"
                     /translation="MSGARRLGLLGSGEFEPWAEEVDRWLLDGASDGRVLVAPAASAA
                     EGEEVFSRWGRRGVAHYERLGYAAEVLPLKTRDDAADPRLASRLDEASMVFFSGGNPA
                     DLAATLAGTFFWDRLLQRLGEGLAFGGCSAGVAFLGELAPDSSIADPPGGDPWRPGLS
                     AFGGVVLAPHWDTVDAHVPGFRTAVERMVGPGLVLVGVDERTALIGDGRTWRVTGEGS
                     VHVRFQDAWTSYPPGRTLPSGVLGLTSAARPA"
     gene            complement(19664..19849)
                     /locus_tag="VF097_02285"
     CDS             complement(19664..19849)
                     /locus_tag="VF097_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261656.1"
                     /translation="MQLLLGLVILQFGLGILLAGVAGRVRRDAGGSFWAGAALSALLG
                     LVGFWIVVASARRASRS"
     gene            complement(19881..20090)
                     /locus_tag="VF097_02290"
     CDS             complement(19881..20090)
                     /locus_tag="VF097_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6261657.1"
                     /translation="MTNGSANAEYLKTAEVAEILHVSPKTVTRWAKDGKLPHSRTLGG
                     HRRFPAEAIRRLAEGLVQVDEIETP"
     gene            complement(20188..20349)
                     /locus_tag="VF097_02295"
     CDS             complement(20188..20349)
                     /locus_tag="VF097_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261658.1"
                     /translation="MPRPSKLEAAGRVCAAPGCETGLSIYNAKSTCWQHTDLTFPNFR
                     GKRLAKGSA"
     gene            complement(20441..20695)
                     /locus_tag="VF097_02300"
     CDS             complement(20441..20695)
                     /locus_tag="VF097_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX6261659.1"
                     /translation="MSGTECEEILRQVEVYLDGELPAEEAARVEGHLAGCSPCLERQE
                     FVSNLRRILREKCGGVERLPDAARERIHRTVAREAASETD"
     gene            complement(20682..21302)
                     /locus_tag="VF097_02305"
     CDS             complement(20682..21302)
                     /locus_tag="VF097_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6261660.1"
                     /translation="MPDRETSEPPRSVTTVRTDASREELDRRFELEAIPLLEDMYGAA
                     MRLTRNPADAQDLLQETYLRAYRGFAGFEQGTNLKAWLYRILTNSYINLYRKRQREPQ
                     TVSDDEVEEWYLYSRLRESDLEPSAEAEVIERLPDEDVQAALASLPDVYRMAVILADI
                     EGFSYKEIASILDVPIGTVMSRLHRGRKALERALWDIVRERGVVRD"
     gene            21365..23377
                     /gene="ftsH"
                     /locus_tag="VF097_02310"
     CDS             21365..23377
                     /gene="ftsH"
                     /locus_tag="VF097_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX6261661.1"
                     /translation="MGDDERRGRWQGFDFGGGSGDGRRWRFPIGYLLLAFLLLVFLQS
                     LFVRSTDEISYSRFLALVQEERIEGLVRISSGSIEGEFQNQEGDVERFQVIRPPDVQD
                     EQLISLLQENGVEFTGEQPSGFVNFLAVWGPFILIFGLMFLFFRRMAGGGAGSALNLG
                     KKGVKIYDRKDLKTTFDDVAGLDEAIEELREVVDFLANPKKYQRLGGRIPKGVLLMGP
                     PGCGKTLLARAVAGEAGVPFFYMSGSDFVEMFVGLGAARVRELFQQAKEKAPVLIFID
                     EIDTLGKSRSGAMGGSLGSHDEREQTLTQLLSEMDGFDTSKGVIIMAATNRPDVLDPA
                     LLRPGRFDRQVVVDRPDLKGREEILKVHAKDVVMSPDVDLHLLAARTPMLTGADLANI
                     INEAALLAARREKDAVGMAELEEAIDRVTVGVERRSKVMSDRERELSAVHELGHALVA
                     LDLPGTNVLHRVTIIPRGRAGGVTYFRPTEDRTYHTDAELNDQLAIALGGRVAEGIAF
                     GDVSTGAHNDLANATQIARAMVIEFGMSEKVGPISFGNDGFRDAQGRPLFPGADRPNI
                     SDDTMRLIDEEVTRLLTQARERARSILEPKKDLMLRLAKVLMARETIEGRELKEWVDG
                     EEPIPEPGEEPRAEETTRRLEPSEAEGDGEAVGDGARETEEIHRPT"
     gene            23374..24243
                     /locus_tag="VF097_02315"
     CDS             23374..24243
                     /locus_tag="VF097_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX6261662.1"
                     /translation="MSRLSGTLAASVTPLEDGGARPDEDAVGPVVDFLHQGGVDGILA
                     LGTTGEGIMLSGEERRRVAERFVEASAGRLRVAVHCGAQTTSETAALAAHAAEAGADA
                     VAVISPPYYPFEPVGLLRHFREAAAAAAPLPFYVYEFADRSGYAVPVEVLSDLRGVAD
                     NFLGMKVSDAPLERFAPYLLEGLDVLVGPEALILEGLGRGAIGAVSGLASAFPELVAE
                     LVRSRDPALGERVGALRARLQRLPVPAVFKAALRLRGVPVREDVRPPLRTLTDEERAE
                     VAAAVEGAATPAG"
     gene            complement(24227..25117)
                     /locus_tag="VF097_02320"
     CDS             complement(24227..25117)
                     /locus_tag="VF097_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261663.1"
                     /translation="MSEPPGEVRRLAEERAEARAARDFDRADALRERLVALGWEVRDA
                     PDGYDLSPAGPAPRVPPEAVPSRLEEPPTHDLTVHWLAERWWDDVLRGVASFRRYEGD
                     RRVEHLVVEADPAPPGTWPEGVRVLPLDRDPGFGSARAAGLRQTMGRIAIVADASVEA
                     VGDPYAPIERALADETIGVCGPVGAVTRDLRSFEESIAPDVDAIQGYLMALRRDLLAR
                     VSFDPGYRFYRWADVDLSFEVKALGLRAVRIDVPIRRHEHRGWAALDPAERGRRSRRN
                     HARFLRRFGERSDLLVNPPA"
     gene            complement(25114..26118)
                     /locus_tag="VF097_02325"
     CDS             complement(25114..26118)
                     /locus_tag="VF097_02325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX6261664.1"
                     /translation="MPDIGIVVGGHMSLIADLAKAAEAGGYESVWVAETSHTPFVQAA
                     VAAQSTSRVTIGTAIALAFPRSPTVVASTARDLQELSGGRFVLGLGSQVKRVNVQRFG
                     VPFEHPAPKMAEYAEVVRGVLATFDGEPIDHRGRFYEITMPPFPGASSVPRIPLYLAG
                     VNEKMIETAGRVYDGLLGHPMTSPAYVAEVVRPAVERGAAAAGRDPSEVDLTTSVIMQ
                     VHDDVETARRQAAYQVAFYATTRTYKPVLALHGFEELSAPLREAHAARDLERMADLAM
                     SMVDTYAICGPPEACREQLAAYDGLVDRIVLGGAWVGPDRSAIVENYRRILSDFTPHG
                     "
     gene            26258..26716
                     /locus_tag="VF097_02330"
     CDS             26258..26716
                     /locus_tag="VF097_02330"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEX6261665.1"
                     /translation="MPTLYEWLKFAHVLLAIIAVGFNASYAIWLARAARHPERESFAL
                     RTIKTLDDRFANPAYGLLLVTGLLMIWNADIPISTFWISTSIGIYVGVLLLGLGVYTP
                     TLRKQIRELEDDGPRSPAYQRLSTRGTVVGILLGVMVVVIVFLMVTKPTL"
     gene            26721..27440
                     /locus_tag="VF097_02335"
     CDS             26721..27440
                     /locus_tag="VF097_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEX6261666.1"
                     /translation="MDARAAAQELERRLGAVARAERAEQERRYLKSSLRHLGVSVPAI
                     RRTALGFLEEHELDRSELLALARALWKEPVHERRMAAVEILAERADLLRPDDLPFVEE
                     LLRESRTWALVDPLAIQVAGSLVDRWPELGSTLDRWAGDDDFWIRRSALLALLPGLRA
                     GAGSLNRFLGYADAMLEEREFFIRKAIGWVLRETGKRRPGVVARWLGPRAGRASGVTL
                     REAVKHLSEGQRERILRAAGR"
     gene            27453..27890
                     /locus_tag="VF097_02340"
     CDS             27453..27890
                     /locus_tag="VF097_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261667.1"
                     /translation="MMRNRVPALALLVALVAAGCGGGEETVEVTLQEFAVLPAQDTVS
                     AGSVTFDATNEGPDDPHELVVIQTDLAPEELPTTDEGGVDEEGEGIEVIGEIEEFPVG
                     ETESATFDLDAGDYVLICNIVEEEDGELEAHYELGMRVGFTVE"
     gene            complement(27898..28986)
                     /locus_tag="VF097_02345"
     CDS             complement(27898..28986)
                     /locus_tag="VF097_02345"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX6261668.1"
                     /translation="MSVPTTMRAARLHSVRSDDERELISVDEVPVPEPAPGEVLVRIA
                     ACGICASDLHVVDGTTPGGPTPLILGHEAAGVVEMLGEGVTSWSVGDRVAVLMARACG
                     ECPACAAGRESLCARLEIPGIDGDGSQAEYAVARDRDLLPVPGGIPLEQAAILTDAVA
                     TPFHAMRRSGFEPGMSCAIFGLGGLGMHALLLARALGASWVAGVDLDPANLERALAWG
                     ADETIDADDGKPAREIRGRSGGGVDRAFEFVGSPASQSQAVKSLRPGGRATIVGLTPA
                     PLDLLPSALLVSQELEVVGSFGSSPRDLEELLAMVERGDLDLGRSVTHRWPLEAFSEG
                     LRALETREGHPIRSVVVYDEEPGAAPPR"
     gene            29031..29801
                     /locus_tag="VF097_02350"
     CDS             29031..29801
                     /locus_tag="VF097_02350"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX6261669.1"
                     /translation="MQRIVETIVLTEEDLRVLGETDFGVPGLIAHEWIGPFVEVQAVG
                     PLIMVHDGIFDPRKGITHHPHRLNERLFYILEGTVDHDDALNGITGHMGTGDLGRLTE
                     GRRGMFHQEWNNTDGRARAFILVYGTDPVPPTASFQALRDADAPRYQAFPGVESKEIV
                     GPKADLPLHGDVRLWTDDRMEQESESALELEEGEGGLVFPIEGAVVVDGSELRPEAMA
                     VLPPGGSSRSVAVSSPEGARVLRVVFGPGYGLALRSAT"
     gene            complement(29798..30112)
                     /locus_tag="VF097_02355"
     CDS             complement(29798..30112)
                     /locus_tag="VF097_02355"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HEX6261670.1"
                     /translation="MRAQHVVLFKFARPLSEDEEREMFDRVRAWPETIPGFTGLRLGR
                     DVSGRNEGYEYLLLTEFESEEAHQAYYSQPAHTSFSEWVFSRDCEVIRVDYPLDDRNL
                     IV"
     gene            30160..31341
                     /locus_tag="VF097_02360"
     CDS             30160..31341
                     /locus_tag="VF097_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX6261671.1"
                     /translation="MRATTDWGFARRTRVDVGEGLASILALSGATDLISFAGGFPDPE
                     TFPTDELAALVQRVSQDPAALQYAPTPGLPGPRAFLADRLETREGQRPSDEELLITSG
                     GIEALELLGKSLIDPGDLVLVEGPTYLGAIMAFRSFEADVVAVPMDDDGLDVGRLEAT
                     VRERGRPKLLYTIPDHQNPAGVSLAEERRSALVEVARRHGFLVVEDVAYRELGFTEDR
                     PPSLWALGPDVVVQAGTFSKTFFPGVRLGWAAGPAEVVAQMVNAKQNTDQCAGALGQR
                     LLEEYGRRGLLDEQIDRARGIYRSRRDTAMSALERHVGDRATWTHPHGGFFTWLELAE
                     DGADVARRALQAGVAVVPGRPFFSGVGGERNIRISYSQASEERLEEGIALLGPIVRAR
                     A"
     gene            complement(31325..>31537)
                     /locus_tag="VF097_02365"
     CDS             complement(31325..>31537)
                     /locus_tag="VF097_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX6261672.1"
                     /translation="LVERCVSDLAGAGVQFLSVLTVVESEPDWRETDNYADTRAFYRA
                     LGFLPLRELALEGWDERAVILVRPVP"
BASE COUNT         4469 a        11471 c        11238 g         4359 t
ORIGIN      
        1 cgcgggactc ctccttggcg tcgggggcca cggcgtcctc gaggtcgagg aagacctggt
       61 cggcggggag gctcgccgcc ttggccagca tcttcgggct ggagcccggc accgcgaggc
      121 acgagcggcg gggccgggtg acgcgctcgg tcatgcgtgg gctccttctg gtcaggacgg
      181 gctcatggta gacggcccgg tcgcgggacg cccaggccga tcaggcgcgc gggggctggg
      241 actcgacccg gccgatgcgc tcggcgaggt gctcaacccg gacggagagc tcggtgagct
      301 gggtggagag cttccctccc aggtcggtga ggtccctgac gaaccgctcc tgctgctcga
      361 cggccttccg ctcgctgcgg atcaggtcct tgaacccccg cttctgcgcc agcgactcca
      421 ggacgccgga gagggcgtcg aggcgcgtga tgcgctggtc cacgcgggcg gcgagggcgg
      481 ccacgtcgcc gcggatcctc tccaccgcac cgttcagggc ctcgagctgg cggcccacgg
      541 cctcctcggt gccggcgatc tcggccacgg cctcctgcat gaccttgagc gccctggcca
      601 catgctgcgg gagggtctgg accacctcga ggtcaccgcg aatcgaggcc gccgcgttgc
      661 gcacggcgtc ccgcacggcg agcggtgact gctcgagcag ctccgcgatc acggtgaggt
      721 gagccgtgac ccgctcctcg acgcccgcca cggcgctcct cagaccctcg atgcttccgg
      781 cggtgcgatc ctgaaccgcg tcgagggccg ctcggatcct caggccgaag acgtccgaga
      841 gctcggccgg gatcgtggcc agacgccggt caaggtctcg ctcggtcccc cggtgccctc
      901 catccggggg atcgggatcg tcggggatct cgaagagccg gtcatcgtcc atgctcggcc
      961 ggattgtatg tcccgaaccc cttccgaccg gggatttccg gggagggggt tcacaacctg
     1021 cctcaagcat gagataggtg tggacgaaac cctctatagg gacccatgat gacgcgcccg
     1081 tccatccgca tcctcatcgt cgcgctcctc gccgcggggt tcgcgctggg cctcctgccg
     1141 cccgcgggcg gctccgggcc tccggagacg atgctctcca cggttcccct gcacgaggcc
     1201 gagctggccg ggcgtgggct cctggcctcc ggccaggccc tctcggccac gcggacgtcc
     1261 cggacccccc gccacgaggt gtgcgcggac ttcgccttca cggcggtggg cctcacctgg
     1321 aggcagaccg gggacgagct cgtgccggtg agcttccgat gggacgaggg aagcggcgtc
     1381 cgtggggtcg ccgtctacgc ggaccccgac cacgcccccg acccgggcac cggcgagcgc
     1441 gcgcggggtg gaaccccgct cgtgtggacc ggcgaggcgc ggtgcgtcga gctgacgctc
     1501 gagctgcagg ccggcgtgga ggtccgcgac ctgcgagccg tcttcgtcaa cacctcgggg
     1561 acgtcgcggg aggggtcgat cctggggacg atcgccacgg ggatcggccg gggcatggcc
     1621 ctcctctggg gcatggagac ctcggaggcc gtggccccca agcccaagat catccggcgc
     1681 agcggatggg gggcgaacga gagcctccgg aactgcggcc cctcgtacgc ccccgagctc
     1741 aagatggcct acgtccacca caccgcgagc gggaacgggt actcgaaggc gcaggccgac
     1801 gacgtgatcc gcgccatcta cgcctaccac acgcagggcc gcggctactg cgacatcgcc
     1861 tacaacttcc tcgtggaccg gttcggccgg atctacgagg gccgcttcgg tggcatgacc
     1921 gagccggtga tcggcggcca cgcgatgggc ttcaacacgg ggagcaccgg ggtcgccgtg
     1981 gtcgggaact tcgtctcggt taatccgggg acggccgcga tcagcgccct gaagcgcctt
     2041 ctggcctggc gcctcgatat cgcgcacctg aacccgaagg gcacggccac gatggtgtcc
     2101 gcgggcgggc cgaaccagaa gtacgaggag ggtgaggagg tgaagctgcc ggtgatcgcc
     2161 ggtcaccggc atacggggta cacgacctgc ccaggcgacc gcctcttcgc caagctcggg
     2221 gcgatccgca cgggcgccca ggcacggggc ctccccaaga tctggaaccc gcgggccacg
     2281 agcgacaccc tggagctcgg ccacgggggc gtccgctacc gagccaagct ctccgcagcc
     2341 ctgccctgga gcgtggagat cgccggtccg agcgggatcg tgcggacctg gacgggcacc
     2401 ggccaggggg tcgacgtggt gtgggagggc ttcgacgatc gagggttccc ggcggacgag
     2461 gccgactaca tcgtcacgat gcgagcgggt ggcgccgggc agccgcaggc ccgaccggcc
     2521 gcgctccgga ccgcgctgac cgggtcctgc gacatcacga cgacccgctc ctccccgacg
     2581 gcgatcggga ccgacgagga cgaggtcctc tgcggcctca acgggcaggc cgacgtcctc
     2641 gacggcggcg gaggcaacga catcctcatg ggcttcgggg ggaacgatca gctgaccggc
     2701 gggcccggac aggacaccct tgtcgggggg aacgggtcgg acatgctcca gggcgggacg
     2761 ggggccgacg tcctgttcgg aggggccgcg aacgacacgc tcgtgggggg agcgaacgcc
     2821 gacgtcctcc acgggggacc gggcgacgac cagctcgacg gaggaggcgg cgccgacacg
     2881 gtgagccacg ccacgtcgtc cacggcggtg cgggtggacc tggccgcggg aacggccatc
     2941 ggcgagggga cggacgacct cacggggttc gtcaacgcga tcggctcgcc acggaacgac
     3001 acgctcatcg gtagcgacgg ccacaaccac ctcgtgggcg ggggaggacg ggacgtcttg
     3061 accggaggcg cgggagacga cctcctcgag gccggccccg gcgccgaccg ccttgcgggc
     3121 ggcgccggag acgacgctct cgtgggaggc ggcgggagcg acaccgcccg cttcgcgggg
     3181 gcgccgggcc ccgtcgtggt cgatctgcga gctggctcgg cgatcggcga cggcgccgac
     3241 acgctgcgcg cgatccagaa cgtggtgggc ggccccggga aggacacgct caggggcgac
     3301 gcaaaggcga accgcctgct ggggaagggc ggctgggaca tcctcgtggg gaagggcggg
     3361 cccgacctgc tcgtaggcgg ggccgggcgc gacgacctgc gaggcggctc cgggagggac
     3421 acactccggg ccaaggacgg gaagcgggac tcggtggacg ggggcggcgg gttcgacgcg
     3481 gcgcacgtcg atccgaagga cgacgtgcgc cgcgtcgaac agcaccgctg attcagcgcc
     3541 gggccgcggt caccgactcg gccaggacct ccagcccctc gtcgagctcc tcgtcggaga
     3601 tcacgagcgg cagcaggtgg cggaggacgt tcttgtagat cccgcagttc acggcgagga
     3661 gcccgcgctg catcgcctcc ccgatgaccc ctcccaccag ctccttgtcg ggctccttcg
     3721 tctcggggtc cttcaccatc tccacgccga tcatcgcccc gatgccgcgg atgtcgccga
     3781 tctcggggac gtcgcgcgcg atctcctccc acgcggcgcg gatcgtcttg cccacgacct
     3841 gggcccgctc gaccagctcc tcctcctcga tcacctggag cgtggcgttc gccgccgcgc
     3901 aggcaacggg gttcccgatg taggtgccgc cgatctcgga ggggccgggg gcgtccatga
     3961 cgtcggcccg tcccacgacc gccgagatcg ggtaccccga tcccatcgcc ttggccagcg
     4021 tgatcaggtc cggttccacg ccgaactgct ccaccgcgag gaaggtgccc gtgcggccgc
     4081 accccgtctg gacctcgtcc gcgatcagga ggatgtcgtg ccgcctgcag atctcggcca
     4141 gcccggggag gaagtcgggg gtgacgggca cgaacccacc ctcccccagc accggctcga
     4201 tgatcacggc ggcgacggtg tccggatcca cctgcgtcgt cagcgagcgc tcgaacgcct
     4261 cgagggcgag cgccccggcg ttctcgggct cggggctccg gtacgcgtag gggaacggga
     4321 cccggtacac ctcgggggcg aagggcccga acccgctctt gtagggcgcg tggcggctgg
     4381 tgagcgtcat cgccatcagg gtgcggccgt ggaaggcgcc ctcgaaggcg atgatggccg
     4441 agcggccagt cgcccgtcgc gcgatcttga ccgcgttctc gacggcctcc gccccagagt
     4501 tgaagaaggc ggccttccgg tctccgccgc ccacggcggc cacgaggcgc tcggccagct
     4561 ccacccacga ctcgtacggc accacggtga agtcggtgtg ggcgaaccgc tccagctggg
     4621 cccgggcggc ctcgacgacc ttggggtggg agtggccggt gaggagggcg ccgagcccgc
     4681 cggcgaagtc gaggaaggtg ttgccgtcca cgtccgtgaa cgacgccccg tgtccctcct
     4741 cgatgaagcc gggcatgtgg aggtcgaacg gctccgcgac gacctgctcc ttccgttcga
     4801 tcagctcgcg ggacctcggc cccgggatct cggtgaccag gttgatcgtg cccaactccc
     4861 cctcctccct ctcgcgcgcc tcggaggcta ccagaggatc acagacgcag aggccgcggc
     4921 gggctcatga tcgcggccgg atcctcccgc cagcgctccg gctggacgtc tatgtacagc
     4981 tcgatctcgt tgccgtcggg gtctgcgatg tagacgctgt gcgtgacgac gtggtccgcc
     5041 atgcccatga tctggacgcc ggccgcctcc agcgcccgga tcgcctcgcg cagctcgtcg
     5101 tcggtcgtgc ccaccttcag cccgaagtgg tacatgccga cgcgcctgcc gggcgggacc
     5161 ggcgtggcgt cctcccccac ctccagcagc agcagctcgt ggtgggtgcg gccggacgag
     5221 aaggccgcgc cctggctgcc gaacctgccg atctcctccc accccagcac gtcgcggtag
     5281 aagcggaccg agcgctccag gtcccggacg tagagaacga gatggccgag ctcgtgcacc
     5341 ttcatcgagg cctccttgac gcgtgagcct ccaggatagc ctgggaacat gccgtcctct
     5401 cgcggtgttt ctccgaatgc ccgcgtacgc ttgacgagga gcccatgacg aagctgatcg
     5461 aggtcgaatc cctcacgaag gtctacccgg acggcacccg tgccgtggac gacgtctcct
     5521 tccacgtcga ggagggagag atgttcggct tcctcggccc gaacggggcc gggaagacca
     5581 ccaccatccg catcctcgtc accctgctcc ccaagacggc cggctcggcc cgggtgggcg
     5641 ggatcgaggt gagcgaggac cccgacacgg tccgccgctc gatcggttac gccgcccagt
     5701 tcatcggagt ggacgacgac ctgaccgcgc acgagaacct ggtgctccag gcccgcctgc
     5761 acgggatcgg caaggccgag gcccggcggc gcgcggagga gctgctcgag gtgttcgctc
     5821 tctcccccgt cgccggcagg cgcgccgcga ccctctcggg cgggatgcgc cggcgcctcg
     5881 acctggccca ggccctggcg caccggccgc cgctcgtctt cctggacgag ccgaccacag
     5941 ggctcgaccc gcagacccgc aacgcgatgt ggcggtacct cgaggagctg aaccggggcg
     6001 ggaccaccgt gttcctcaca acccagtacc tggaggaagc cgaccgcctc tgcacgcggc
     6061 tggcgatcat cgaccacggg aggatcgtgg tcgagggatc ccccgccgag ctgaagaagc
     6121 aggtgggggg cgacgtggtc acgctctcgc tggaggacca cgagcccgag acggtgcggc
     6181 gcgcgctcga ggccgtgacc gcgttccccg gggccgggga gccgcgggtc ttcgaccact
     6241 cgatctcgct cccagtgaag gacgccggcg aggcgctgtc ggcgctcgtg cgccgcctgg
     6301 acgcagcggg catcccggtg tccaggctgt cgatgtcctc gcccaccctc gacgaggtct
     6361 tcctccgcca cacgggcgag cggatgcggg tggaggaccc gaacacgagg gcctcgagcc
     6421 cggcgttccg cgtcatgcgg gggaggcgct gatgcgcacc gtggccgagt cggcgatcct
     6481 cttccgccgg aacatcacga agctcgtccg ggttccgatg atgatcttct tctcgctctt
     6541 ccagcccctg ctgtggctga tcctcttcac ccagatcttc cagcggctcg gggagttccc
     6601 gcagttccag cagcagggct acgactccta cctcatgttc ttcgctccga gcgtcctgac
     6661 gatgacggtg ctgacctcgg ccttccagtc gggcatgggg atggtcaccg acctcgagac
     6721 gggcatgctc gacaagttcc tcatcagccc gatccggcgg tcgagcgtcc tgctggggaa
     6781 ggtgatggcc gacggcttcc ggatgctgct gcagggggcg acggtgctcg tcatcgcgct
     6841 cctcatgggg gccaggatcg agaccgggat cccgggagca tcgatcatgc tggtcgtcgc
     6901 cgttctgttc gggatcgtct gggccgggct ctcgaacatc gtggccctgc ggacgcggca
     6961 cgcggagatg acgatgatga tcgggatcct gctgacgttc cccctcctct tcctctccac
     7021 ggcgatgatg ccggagggcc tcctgcccgg ctggctggag acggtggggc gcttcaaccc
     7081 ggtgtcgtac gtgatcgaca cggcccgcgc gttcatgaac gacgggtacg actggggtca
     7141 gctcgggatc acgctggccg tcatcgcggc ggtcggcgcc ttcacgctga ccggcgccac
     7201 gcgcgccttc cgccgcgcca ccacgtgacg acggtctgga cggtcggtca cagcacgcac
     7261 ccgatcgaga cgttcctcga gctgctgcgc ggggccggca tccggcggct cgtggacgtc
     7321 cggaccgccc cggggtcccg gaggcacccc cagttcggac gggaggcgct ggccgcgtcg
     7381 ctggagcagg cgggcgtcgg ttacgagtgg cgaggtgagg agctcggcgg ctggcggaag
     7441 gcccggccgg actcccgcca caccgccctg cggtcggccg ggttccgcgg ttacgccgac
     7501 cacatggagt ccgagagctt ccgcgaggcg ctggacgggc tgctcgacga ggctgaggag
     7561 cggcccacgg ccgtcatgtg cgccgaggcg ctctggtggc ggtgccaccg ctcgatgatt
     7621 gccgacgcgg tcgcggcccg gggccacgag gctcgacacc tgatgcccga cggctccatc
     7681 gccccccacc ggccgaggtt ctcccgggtc gagggcggcg tccccgtcta cgatccctag
     7741 gcgcgccggc ggacctcgtg ctcgacgtcg ccgaatgcgt cgagaggggc gatcacgtcg
     7801 ccgagcacga ggacgagcga ctcgaagtcg agcgagggcg cctcgagcct gagccactcg
     7861 gaccttcgcg ccgcctcctc gatcgccgac cggcaccgcc gggccaggtc gtcgggcgca
     7921 ccctcccagg ccccgagcac ccgggccgcc tcggccagcg cgtcctcgaa ggccaggatc
     7981 gaggtggcca gaggctcggg ctcgcggcgg gaggcgggca cggcggccac gagggcgtcc
     8041 ttcgctcgct ccacctcggc ggccacccgc tcgaacccgc gcgggatcct tcgtcgcctc
     8101 cccagggcca tgcggccgag tctacgggtt ccatctgggc cctccttccg tgagaatggc
     8161 gcgatgcggc tcctcctggt cgtcaacacg cgggcgggca ccgtcacgcc ccgcacggtg
     8221 cggttcatcg agcgagccct gtcggatcag gccaaggtcc ggatcgagct gacgcgggag
     8281 ccgggccacg gggtcgagct ggccgggaag gcggccgcgg gcgatcacga cgtggtggcc
     8341 gtgatgggcg gcgacggcac cgtcaacgag gtggcgaacg ggctcgcggg ctcggacgtt
     8401 cccatggcca tcatccccgg agggggcacg aacgtcctcg cccgctcgct cggcatcccc
     8461 caggagccca tccgcgccgc ggggttcctc ctggccaggc tctcggggcc cggccgccgc
     8521 atccccctgg gtcgggcggc ggaacgctac ttcaccttct cgtgcggctt cgggctcgac
     8581 gccgagatcg ttcgccgcgt ggagcgcaga cagcgcctga agcgggccgc cggccacggc
     8641 tacttcgtct ggaccgcgct ccgagtgggc ctcgtctcct acgacttcaa gacgccgagg
     8701 atccgtgcac gctggggctc cgcgctcgag cacgagcgag acggcctcac gttcctcatc
     8761 tcccagaacg tcgacccgta cagctacctg ggctccctcc ccatcaaggt ctgccccagg
     8821 gcgagcctgg acctcggcct ggacgcgttc gccggcgggc cggtcggcag gatccggatc
     8881 gtccgctggg ccctcaagac cctgggaagc gggcgccacg cgcgcgaccg ccgcgccctc
     8941 tcgctccacg accaggggcg gatcgaggtc gtcgcggcct ctccgcaccc cgtgcagatg
     9001 gacggggagt acgtcggcga ccacgaccgc ctcctcctgg agtcggtccc cggggccctg
     9061 tcgatcctgg cgtgaggatc aggtgccgag cgcgcgctcg cgcgcggcca cggcgagcgc
     9121 cggccggttc gtggccacgg cggcgagccc gcgggcgaac aggcgctcca tctgtccctc
     9181 ttcgtccacg gtccacgtgc ccacctcgat acccagccgg gccgcggcat cgagcaggtc
     9241 gtccccaccg tgatcgaaga ccccggcgtg tgggaggagc cagcgatggc cgtcctcgtg
     9301 agccaggtcc agcccgagca gctcgccccc cggcagaacg agcagtccgg tggggacgtc
     9361 cggtgccgcc tccacagccg tgcggagggt gggcggatcg aacgacgtga tcagcccggc
     9421 tcccgggaac ccctcaagcg cctccgtgac tgcctcgacg atctcgtcgc cccctggccg
     9481 gtacgagggc tcgcccggca ggttcttgat ctccacgtcc acccccgtcc ccgtcccctc
     9541 gagcagctcg aggacctctc cgagcgtcgg gacctcctcc cgcggtcccc gccctcccga
     9601 cgcatcggcc tccttcacct gggccagccg cacctcatgc accaggcccc gcgcgtcggt
     9661 ggtccgctcg agctccgggt cgtgcagaac tacgacggcg ccgtcggccg tgagtctcgc
     9721 gtcgagctcc acgagatcgg cgccgacctc cgccgccacg gcgaaggccg tcagggtgtt
     9781 ctccgggtga tcggcggagg ccccacggtg ggcgatcacc agggggcggc ggggttccac
     9841 gtccggccct ccgatgcgta cgagaaatag gtctttacac gagcggcaga ccgacactat
     9901 actgccgcac gcgccggccg gggcctctct tcaccgggcc ttgtgaagcg cttcacgaga
     9961 ccgggccaaa ggcccgggaa ggggacggat ggactgggta cagcgggcga ggtgcaggga
    10021 cgaggacccc gagctgttct tccccgtcgg gacctccggt ccggctctcg agcagatcga
    10081 gcgcgctcgc gccgtctgta tgcagtgcga agttcgtgaa gagtgcctcg agtgggccct
    10141 ggcgtccggt caggacgcgg gggtctgggg aggcacctcc gaggaggagc ggaaggcgat
    10201 gcgccgcacc cgccgtctcg ggccgcgggt gccggttctg cgctgaccgc cgtgcgggcc
    10261 ctccccgagc ccaaccacga gccgatcggc ttctcccacg aggtcttccc gacctctcgc
    10321 cggtggctgg gtctggccct gctcatgatt ctgacaattg cgacggccgt cgccgcggcc
    10381 gtccggctcg tcgggatcgc ggatccgtga ccgtgggtcc gccgcgaccg aaggaggagg
    10441 gaatgtgaat gcgctcgccc aagccctgcc catgccccga gcccgaggcg ctcgactcgc
    10501 gttccaggcc gcgttggcga tcggcgggag cctgctgatc gcggcactgg cccaggtctc
    10561 ggttcggctg ccgttcacgc cggtgcccat cagcggacag accctcgggg tgctcctcgt
    10621 cggcaccgcg ttcggctggc tgctcggggg gctcaccctg gcgctgtacc tggccgagat
    10681 cgccgtgggg ctcccgttcg ccgccgaggg caacgcgggg tacgaggtgc tgaccctcgt
    10741 cacccccagc ggcgggtaca tctgggggtt ccttgcggcc gcgatcctga tgggctggct
    10801 ggccggccga ggatgggacc gcagcatccg ctcctcgatc agcgtgatgc tgctggggca
    10861 catcgtcatc tacgtcgtgg ggatcgtgtg gctccatgcc tcaccgacgt tcgccgcctt
    10921 cctcggccac gagccgagcg tcaaggagac cctcaccgcc ggcctctacc cgttcgtcgt
    10981 gggcgacgtc ctcaagctgc tgctggcggc cggcgccctg ccgctcgcgt ggcggctcgt
    11041 ggggaggcgc ggcgactaga cccggctcgc cgggcagagg tggctgacga cgcaatcctc
    11101 gcaccggggt cgcttcgcgt cgcagatccg tcgcccgtgc tcgatcagca tgtagctgaa
    11161 accggaccag tcgtcccgcg ggagcagggc catcaggtcg cgctcgatct tgtcggggtc
    11221 tttctgtgag gtgaggccca gccgcccgga gacgcgtttc acgtgggtgt ccaccgcgat
    11281 gcccgcgtcc gggtcgtcct tcgccttctc cggataggcg ttccagagca cgatgttcgc
    11341 ggtcttgcgc gcgacgccgg gcagccgcac gaggtcgtcc atcgagtcgg ggacctcgcc
    11401 gccgaactcc tccacgatca tccggcaagc cccgcggacc gccttcgtct tctggttgaa
    11461 gaacccggtc gggcggagat cctccttcag ctcgtcatcg gggaccttca ggtagtcctc
    11521 ggccgtccgg tacttctgga agagggtcgc ggtcacctca ttgatcttct tgtcggtgga
    11581 ctgcgcggag aggatcgtcg ccaccaggca ctccaggggc gtctcgaagg cgagggccac
    11641 gcgggcgccc cggtactcgc gcttgagaag atcgatcacc tcggggccgc gttccgcgag
    11701 cggcgactcc ctggtgaggg ccctcgccat cagcgcccgc ggaagtcggg gtctcgccgc
    11761 tcgcggaacg cggcgatccc ctcctgaagg tcctcgctcc cgaacgcgtg ggcggccatc
    11821 atgtcgaagt cggcgacccg gaacccctcg gcctcgcggt cgatcgagag acgagagagc
    11881 acctgccgga tcccacgctt cgaggcctgg acggacagcg gggcggcgtc cgcgatctcc
    11941 tccgcgtaga aaagcgcgcg gtcgggaagg tcctccggcg ggaccgcctc gccgaccagg
    12001 ccccagtcgg ccgcctcctg gccggagatc gtcctcgtcc ccagcagcag ggcgcctgcg
    12061 cgcttgggcc cgaccgcgag cacgagccgc tggatgttct cgtacccgac gaccaccccg
    12121 agacgcccag aggggatgcc gaacgtggcg tcgacggcgg cgatccccag gtcgcaggcc
    12181 acgacgagct ggcatcccgc gccgagcgcg ctcccctgca ccaccgccag ggcaggaacg
    12241 gggaggtcct cgatcgagcg catgacctcc tcgagggcgc ggccgtagtc gatgccgtcg
    12301 ggtccctccg tggcgcgggc cagctcgtcg atgtcggcgc ctgcgcagaa gtgccgtccc
    12361 tcccccgaga gcagaacgac ccgggcgtcc cggtcggcgg cggcatcgcc cagcgcccgc
    12421 atcagctctc ccagcatcgc cgcgttcacg gcgttgcttt ggtccgggcg cgccagggtg
    12481 atccgccaga tgcgccccgg ctgggttcgg acgagcgtca tgcggccatg ctagcgcggc
    12541 ccgcacgggt ggtcagccga aggtgagcac gagggcgggg ccgtcggggc ccgactcccg
    12601 gctgtcgtag gcctgctcga agccctgccc gcccgagccc tcgacctggt ccctcagcat
    12661 gaagccgtgg ttccccgagg cgtacatctc gaggacctgc tggagcaccg gccactcccg
    12721 ccagcccgtt cccgaggggc tcgaggcggg cgaccccgag gtggccggct ggttcgccca
    12781 ggtcacgccg ccctcggtcc agctcgaggc cgcgcggtac gcgaggatcg tccgggcgcc
    12841 ggtcgaggac gtggcgtgca gccgcagcct ggcgtcggtc agggcgcacc ccgccggcac
    12901 cggaggcaga tcgaaggcca cgaacgtccg ccggtcctga gcccgggaga cgacggagag
    12961 ctcggcctcc ccaccgtggt tggtgccgga gggattcgtg acgacccagg cgtcgccgat
    13021 ggcggcgagc gtctgggccc cgcagaagga aggggcggct tggaaggtgt tggcggggtt
    13081 ctccgtgacc gtggagaacg cggcggtcgt cgagccgagg agcgccgcga ccaggatccc
    13141 gacccccgcg gccagcagac gcgacgctcc gcgcacggcc cctcaccctc accagacgcg
    13201 tcggaggagc gccccaccga ccaggatcga gcccccgacg aggagcacga cccgcacggt
    13261 cgcggagccc agccagtgga gggggtatcc gaccatcggg atcgcggacc gaaccacccc
    13321 gatcgtgccc tccgctccga ccgaccagcg ttcctggccg gtgttggcgt cgcccctggt
    13381 gacgaagtcg tagcggccgt gccgccgctc gacgctcacc acccggtgcg tgaccaggct
    13441 gccctccctc gtggcgtcgc ggaacgtgac gacgtctccg ggggcgacct cgcccggcct
    13501 cacggtcttg accacgacca cgtccccggt cccgatcgcc ggctccatgc tcccgctccg
    13561 cacaaccagg aatcggaacc cggccaccat catcgtcacc atcacgaaga ggagaatcgt
    13621 gagcgtcccc aggacgacga gcgagacccc ggggatccgg ggcctcgcgc tcgcccgagc
    13681 ctggagcgtg gcgtccatca ggtgttccga gcctcccagg tgaagctctg ggtcgcgttc
    13741 agcccctggg ctccgccgtc gtcctgcacg gagacctcga agcggtaggc ccgcgactcg
    13801 ccgctcgtcc acgacccgtc gggatcgacg agcgctgcgc cgtaggcatc cgggtagccc
    13861 gccatcgtcc ccgaatagat caggccgccg cccccgtcgg gcgtgaagcc cgcgcacgag
    13921 tcgaacgacg gcccggcgat catcccccgg gtcacggtga gatcgaggta ctgatcgagc
    13981 ccggtgccgc cggtggtgcc gtacagccga acggtggccg gcagcgtgcc cgtgtaggtc
    14041 accacgatgc aggcggtgtc cgagtcgccc ggcctcgcgt tggccaggga cagcatcgcg
    14101 gttccgctgt cgtcgtccgt gatggagacg gtccccgcgt cgaaggcgtt cccggggttc
    14161 tccgtggtcg cgctgaaggc tgacaaggtc ccgaggccca ccacggcgcc gaggagtgcc
    14221 aggatgagca tcgtcaggcc gatcttcgcc cccgtgcgcc tctctcgccg tcccgcggcg
    14281 ggcgatgcgt gtgcgtcggt catcctcttc ctcccgatcg tgcgaaccga tgcgggtccg
    14341 cgcttccaca agggatatcg gagggggagc aagccgcttg atgacgaagc gggagggttc
    14401 tcgggagtgg ttgtcctgtc gggcggatgg gggacgccct ctagggctgt gacccgacgg
    14461 aaacgcctcg tccggaatcg gccgagtcga gggcggcccg gagggccgcg acgaggtcgc
    14521 gcaggccctc cgggccgccc tcctccaccg cctcgaccgc ccggctgccc acgaccacgc
    14581 cgtcggcgag ccgcgcggcg gccgcggcgt gtgcggggga agcgatcccg aacccggcca
    14641 ggagcgggag cgccgtcacc gccctggccc gcctgatcag gtcggggagg aggggcggcg
    14701 cctccccccg gggcccggtg gtgcccgcca cgctcaccag gtacagccag ccgtcggtgc
    14761 gtccggacgc gaggcggatc cgcgtcgtcg gcgtcgtcgg cgccacgagg tgcacccgcc
    14821 tgagctcggg catggcgtcg gagggcaggt cggccacgat gaggctcgac gcacccgcct
    14881 cgagggcttc cgccgcgaat cggtcccagc cgtaggcctc gagcaccgcc gagtacgtca
    14941 tcgggaccag cgggacctcc acccgccgcc gggcccggga caacgcctcc aggcacgcag
    15001 ccgggcccat cccctcggcc agcgcgcgct cccctgcccg gcggatcacc ggaccgtcgg
    15061 cgagtgggtc cgagtggggg aagccgacct cgacgaggtc tgcccccgcc tccaccgcgg
    15121 cctcgaccag gcccgggagg ccccgcgacg ccatcaggta gatcgcgagc gtgctcatcc
    15181 cagcaccgtt cccagctcct tgtccccgcg gccggagagg ccgacgacga cgagctgggc
    15241 atccagctcc agcgcccggg cgatcgcgtg cgcgggctcc agcgcgggga tgatgccctc
    15301 ggcccgcgac aggagccgga acgcctccag agcttcctcg tccgaggccg tcacgtactc
    15361 gacccggccc gactcgcgca gccgggcgtg ctcgggcccc actcccggat agtcgaggcc
    15421 cgcggagatc gaacgcgcat cgtggatctg cccatcctcg tcggcgagcg cgtaggagcg
    15481 cgcgccgtgc agcacggcgg gcctgccccg ggtgaggctc gccgccccct cggcctcgac
    15541 gccgatcagg cgcacgccgg gatcgtccac gaaggcccgg aagaggccga tggcgttcga
    15601 ccctcccccc acgcaggcca cgaccacatc ggggaggcgg ccctccgcgg ccaggatctg
    15661 ctccctcgcc tccctgccga tcacctcctg gagcgtggcc acgaggtcag ggtagggctc
    15721 gggcccgacg caggagccga ggaggtagtg cgtgtcgtcc acgtgctcga tccaatccct
    15781 gatcgcctcc gagacggcct cgcggagcgt ccgggtcccg aacgagacga gccgcacctc
    15841 cgcgccgagg agccgcatcc gctccacgtt ggggtgctga cggcgcgcgt cctcctggcc
    15901 catgtagacg acgcagtcca ggccgagaag ggcgcacacg gcagcggccg ccacgccgtg
    15961 ctggcccgcg cccgtctcgg ccacgacgcg ccgcttcccc aaccgccggg cgaggaggcc
    16021 ctgccccagc gcgttgttca gcttgtgcgc ccccgtgtgt aggaggtcct cccgcttcag
    16081 gtagaggcgc cgctccgggg cgagacgctc ggccgggaac aggggcgtgg gtcgaccccc
    16141 gtacgttcgt cccagccggt ccagctcctg ccggaacccg gggtcggccc tcgcctcctc
    16201 ccaggcctcc gccagctcgt ccaacgcggg gatcagcgtc tcggggacga agcgaccgcc
    16261 gaacccccga ccgatcccga cgcgcatcac gacgccgcct cccgagccct ggccacgaac
    16321 gcgcggatcg tcccgggatc cttgatcccc ggcgcggact cgaggccgcg actggcgtcg
    16381 acggcccagg gccgtaccat gcggacggcc gcggccacgt tcccgggtgt cagccctccc
    16441 gccagcatca cgcgacccgg cacgcgggcc gcgcgaccga ggtgggccgg gtcgtcgcct
    16501 gtccagggca ggtccgggac tcgggcgacg acccggccac gtcgcaggag gaggccgtcg
    16561 cggccccggt ggccctcctc ggacgcatag gcctgcacga gatccgtgcc gatggggcgc
    16621 gtggctccca cgacgacgcc caccgagagc atctcgtcgg ggacatcgag cggccgcccc
    16681 accctgcgcg ggctctcggc caggacgaac ccagccatgt ccgcgccggc ctcggccgcg
    16741 gcggccacgt cctcgtcccg ggtgagcccg cacaccttga cgagggggcg ggcgatgagc
    16801 tcacgcagct tggccgcggg gtcgggagcg cgcatcaggg ccgtgcccac caggatcgcg
    16861 tcggcgcccg ccagctcggc ctcggcgccc tgcgcgcggc tcgtgacgcc agactcggcc
    16921 accacgacgc ctccgcgcgc ccgcacgacg aggccgagct ggcgggcgcg gtccacgcgg
    16981 aaggtggaga ggtcccgggc gttgaccccg atcgggtcgg ccccgagccc ggccgcccgc
    17041 cccagctcgt ccccatcgtg cgcctccacg agcgtttcca gccccatctc ctcggcccgg
    17101 gccatcagcc ggcgcgtcgc ccgatcgtcc agatcgcgca gcaggaggag ggcggcgtcg
    17161 gcgcccgccc ggcgcaccgc ctccaactcc tcctcggtgc ggaagaaccc cttggccagg
    17221 atgggcaggt ccgtggcggc tcgggcggcg cggaggtccc caagggatcc tgcgaagcgt
    17281 tcgtccacga ggacggacag cgccaccgcc cccgcgcgct cgaaggcgcg ggcgagccgg
    17341 gggacgtccg cgtccggccg cagatcgccg gccgaggggg agcgccgctt gatctcggcg
    17401 atcgcggaga ggcccggggt cgagagcgcg tggcggaacc gcctcacgcc acggcctcct
    17461 cggcgcggct gaacgccacc agcgtctcga gcttctcggc ggccgcgccg ccgtccacga
    17521 cctcggccgc cgcggccagg ccctcccgga ggtccgagac gagcccggcg atccgcagcg
    17581 cggccgccgc gttcagcagc accgcgtccc gcttcggccc agccgcccca tcgaacacct
    17641 cccgaatcgc cgcggcgttc tcctccggcg tcccgccccg gaggtcgtcg ggagcgcatc
    17701 cgtcgatccc gagatcggcc gggtccacga tcgtctcctc cacacgaccc tcctcgacct
    17761 cgaacacgag gttgggcccc gcgggcgaga gctcgtcgat cccgcccgcc ccgtgcacca
    17821 cgagggcgcg gtccgcgccg agcagggcca ggaccctagc tacggtcccg gccagctccc
    17881 gcgcgtacac gcccacgagc tgggcccggg ccccggccgg gttcgtcagc ggcccgagca
    17941 cgttgaagac ggtgcgcgcg gccagctccc ggcggaccgg acccgcgtgc cgcatcgcgg
    18001 ggtgatgcgc cggcgcgaag aggaacccga acccatgctc gtcgatcgac tcggcgatcc
    18061 gctcgggcga gaggtcgagg gagaacccca gcgcctccag gacgtctgcc gagcccgagg
    18121 ccgacgacac ggcgcggttc ccgtgcttgg ccaccccggc tccggcaccg gcggcgacga
    18181 gcccggccgc cgtcgagagg ttgagcgtgc cggccccgtc gccgccggtg ccggcggtgt
    18241 cgatgagatc ccgacggcgg ggagcgacgg ggagcacgtg ctcacgcatc gcctcggcgc
    18301 acccggcgat ctcgtccggg gactccccct tcatccggag gcccaccagg aaccccccga
    18361 tctgagcggg agtcgcctcc ccccgcatga tctcgcccgt cacggcccgc gcctcctcgc
    18421 gggacaggtc cgagccctcc agcaggcgtg cgagcgcgtc cgtgatcgac atctgcctca
    18481 cctccactcc aggaagttgc gtacaaggga tggcccgagc ggcgtcagca ccgattcggg
    18541 gtggaactgc acgccctcca ccggcagtga ccggtgtcga acgcccatga cctcgccgtc
    18601 ctccgcccgg gcgacctcca gcgcgtcggg caggcggcgg gcggcgagcg agtgattgcg
    18661 gccggcgacg aagccgtggg gcgcgcctcg gaacacgccg cgcccgtggt ggcggatcga
    18721 gcgggtgtgg agatcgatgt cggtgtcggt gatcgcggcc aagctcctcc gggaacgaga
    18781 aaggaccccc gatcggaggt cctcgagtgg tgcggctgct acggcgtgat caggccatga
    18841 gcggtcccgc cccacccgag gggcgccagg cccagcgcca ggtgcgaacg ggactcgtca
    18901 tcgtgagcgg aaccttacgc gggccgggcg gcgctcgtca aaccgagcac gccggagggc
    18961 agcgtccggc cgggcgggta cgaggtccat gcgtcctgga atcggacgtg gaccgacccc
    19021 tcgcccgtca cgcgccacgt ccgaccgtcc ccgatcaagg cggtccgctc gtccaccccg
    19081 accaggacga ggcccggacc gaccatccgc tccaccgccg tccggaatcc ggggacgtgc
    19141 gcgtccacgg tgtcccagtg tggcgcgagc acgacgcccc cgaacgcgct cagccccggc
    19201 ctccagggat cgcctcccgg cggatcggcg atcgaggagt cgggcgccag ctccccgagg
    19261 aaggccacgc ccgcgctgca gccgccgaac gcgagcccct ccccaagccg ctggaggagt
    19321 cgatcccaga agaaagtccc ggcgagcgtc gcggcgagat cggcaggatt gccgcccgag
    19381 aagaacacca tcgaggcctc gtcgagccgc gacgcgagcc gggggtcggc ggcgtcgtcc
    19441 cgcgtcttca ggggcagcac ctcggccgcg tacccgaggc gctcgtagtg cgcgacgccc
    19501 cggcggcccc agcgtgagaa gacctcctcc ccctccgcgg cgctggcggc gggcgccacg
    19561 aggacccggc cgtccgatgc accgtccagc agccagcggt cgacctcctc ggcccagggc
    19621 tcgaactccc ccgacccgag gagaccgaga cggcgggcgc cgctcacgat cgcgaggctc
    19681 gccgggcgga ggccaccacg atccagaacc ccaccagacc gagcagggcc gacagggcgg
    19741 cgcccgccca gaaggagccc cccgcatccc gacgcacccg cccggccacg ccggccaaca
    19801 agatgcccag accgaactgg aggatcacga ggccgaggag gagctgcatc tgtcccccgg
    19861 ccggagcccg ctcgtgaccg ctacggcgtc tcgatctcgt cgacctgcac caggccctcg
    19921 gccaggcgcc tgatggcctc ggccgggaac cgacggtgcc cgccgagcgt cctcgagtgg
    19981 ggcagcttgc cgtccttcgc ccatcgcgtc accgtcttcg gcgagacgtg gagaatctcg
    20041 gcgacctccg cggtcttcag gtactcggcg tttgcgctcc cgttcgtcac gtgttcctcc
    20101 gttccgtgga cctacgtccg tcggggcgca gagcgggttt ctcgcaggag tataggtgac
    20161 cttgtgggac tcccgccacc gacgcgctca ggcggagccc ttggcgagcc gcttcccgcg
    20221 gaagttcggg aacgtcagat cggtgtgctg ccagcaggtg ctcttggcgt tgtagatcga
    20281 gagccccgtc tcgcacccgg gggccgcgca gaccctgcct gcggcctcca gcttggatgg
    20341 gcggggcatc cttcccggtc gctttccctg catgacgcga tcggccgggg acgtccggac
    20401 cgctccttgc agggtgatac gccgccgaga cgggctcggt tcagtccgtt tccgacgcgg
    20461 cctctcgcgc caccgtccga tggatccgct cacgggcggc gtccggcaga cgctcgacgc
    20521 ccccgcactt ctcccggagg atgcgccgga ggttcgacac gaactcctga cgctcgagac
    20581 aggggctgca cccggcaaga tggccctcga cgcgggcggc ctcctccgcc ggcagctcgc
    20641 cgtcgaggta cacctcaacc tggcggagga tctcctcgca ctcagtccct gacaacgcca
    20701 cgctcccgca cgatgtccca caacgcccgc tcgagcgcct ttcttccccg atggaggcgc
    20761 gacatgaccg tcccgatcgg gacgtcaagg atggatgcga tctccttgta cgagaagccc
    20821 tcgatatcgg cgaggatcac cgccatcctg tagacgtccg gcagcgaagc gagcgcagcc
    20881 tggacgtcct cgtcgggcag ccgctcgatc acctcggcct cggccgaggg ctcgaggtcg
    20941 gactcgcgaa gcctcgagta caggtaccac tcctccacct catcgtccga gacggtctgg
    21001 ggctctcgct ggcgcttccg gtagaggttg atgtagctgt tcgtgaggat ccggtacagc
    21061 cacgccttga ggttcgtgcc ctgctcgaag ccggcgaacc cgcggtaggc ccggaggtag
    21121 gtctcctgga ggaggtcctg ggcgtcggcc gggttcctgg tgagccgcat ggccgccccg
    21181 tacatgtcct cgagaagagg gatcgcctcg agctcgaagc gccgatcgag ctcctcacgg
    21241 gacgcgtcgg tccgaacggt cgtcacgctc cgtgggggct cggaggtttc ccgatcgggc
    21301 atgctggcaa gcatactcgc ccggagcgct cgtctcccgg cccgtgtggg aagatggtcg
    21361 ggacatgggt gacgacgagc gtcgcggccg ctggcagggc ttcgacttcg gcggcgggag
    21421 cggggacggc cgtcgctggc gcttcccgat cgggtacctt ttgctcgcct tcctcctcct
    21481 cgtcttcctc cagagcctgt tcgtgcgctc gaccgacgag atctcctact cgaggttcct
    21541 cgcgctggtc caggaggaac gcatcgaagg gctcgtgcgc atctcgagtg gctccatcga
    21601 gggggagttc cagaaccagg aaggcgatgt cgagcggttc caggtcatcc gtccgccgga
    21661 cgtccaggat gagcagctga tcagcctgct ccaggagaac ggcgtcgagt tcaccggcga
    21721 gcagccgagc ggattcgtga acttcctggc cgtctggggg cccttcatcc tgatcttcgg
    21781 gctgatgttc ctcttcttcc gacgcatggc cgggggtggc gcggggagcg cgctcaacct
    21841 cgggaagaag ggcgtgaaga tctacgaccg gaaggacctc aagaccacgt tcgacgacgt
    21901 ggcggggctc gacgaggcga tcgaggagct ccgcgaggtc gtggacttcc tggccaaccc
    21961 caagaagtac cagcggctcg gcgggcgcat ccccaagggc gtcttgctga tgggccctcc
    22021 cggctgcggg aagaccctgc tggcccgggc ggtggccggc gaagcaggcg tgccgttctt
    22081 ctacatgtcc ggctccgact tcgtcgagat gttcgtgggc ctgggcgcgg cgcgcgtccg
    22141 tgagctgttc cagcaagcga aggagaaggc gccggtcctg atcttcatcg acgagatcga
    22201 caccctgggg aagagccgct cgggcgcgat gggtgggagc ctcggatcgc acgacgagcg
    22261 cgagcagacc ctgacccagc tgctctccga gatggacggg ttcgacacgt cgaagggcgt
    22321 catcatcatg gcggcgacga accgccccga cgtgctggac cccgcgctgc tgaggccagg
    22381 ccgcttcgat cggcaggtcg tggtggaccg ccccgacctc aagggccgcg aggagatcct
    22441 gaaggtccac gcgaaggacg tcgttatgag ccccgacgtc gacctccatc tgctggcggc
    22501 gaggacaccg atgctcaccg gcgccgacct cgcaaacatc atcaacgagg ccgccctgct
    22561 cgcagcccgt cgcgagaagg acgccgtcgg catggccgag ctcgaggagg cgatcgaccg
    22621 cgtcacggtc ggggtggagc gccggagcaa ggtcatgagc gaccgggagc gcgagctgag
    22681 cgcggtccac gagctgggtc acgccctcgt cgcgctcgac ctgccgggga cgaacgtcct
    22741 ccaccgggtc acgatcatcc cccgaggccg ggcgggggga gtgacgtact tccgtccgac
    22801 ggaggatcgg acctaccaca ccgacgccga gctgaacgac cagctggcca tcgcgctcgg
    22861 aggccgcgtc gccgagggca tcgcgttcgg cgacgtctcc acaggcgcgc acaatgacct
    22921 cgccaacgcc acccagatcg cccgggccat ggtgatcgag ttcgggatga gcgagaaggt
    22981 ggggccgatc tccttcggga acgacggctt ccgcgacgcg cagggccgcc cgctgttccc
    23041 cggcgccgac cggcccaaca tctccgacga cacgatgcgc ctgatcgacg aggaggtcac
    23101 gcggctcctc acacaggccc gggagcgggc ccgctcgatc ctcgagccga agaaggacct
    23161 catgctccgg ctggccaagg tcctgatggc ccgggagacg atcgagggcc gggagctgaa
    23221 ggagtgggtc gacggcgagg agccgatccc cgagcccggg gaggagcccc gggccgagga
    23281 gaccacgcgg cggctcgagc cctcggaggc cgagggcgac ggggaggccg tgggcgacgg
    23341 ggcgcgggag accgaggaga tccaccggcc cacgtgagca ggctctccgg cacgcttgcc
    23401 gcctcggtga ccccgctgga ggacggcggg gctcggccgg acgaggacgc ggtcggaccc
    23461 gtcgtcgact tcctccacca gggaggggtg gacggcatcc tcgcgctcgg caccacaggc
    23521 gagggcatca tgctctccgg cgaggagcgg cggcgcgtgg ccgagcggtt cgtcgaggcc
    23581 tccgcggggc ggctgcgagt cgccgtccac tgcggagcgc agaccacgag cgagacggcg
    23641 gcgctggcgg cgcacgcggc cgaagccggc gccgacgccg tggcggtgat ctcgcccccc
    23701 tactacccct tcgagcccgt gggcctcctc cggcacttcc gtgaggcggc cgccgccgcg
    23761 gcgcccctcc ccttctacgt ctacgagttc gccgaccgca gtgggtacgc ggtgccggtc
    23821 gaggtcttga gcgacctccg gggtgtggcc gacaacttcc tggggatgaa ggtgtccgac
    23881 gcgccgctgg agcggttcgc tccctacctg ctcgaggggc tggacgtgct ggtcgggccc
    23941 gaggctctga tcctcgaggg cctggggcgc ggagcgatcg gggcggtttc cggactggcc
    24001 agcgccttcc ccgagctcgt ggccgagctc gtccggtccc gggaccccgc cctcggggag
    24061 cgggtcgggg ccctccgggc tcggctgcag cgcctcccgg tcccggccgt gttcaaggcc
    24121 gcgctgaggc tgcggggcgt ccccgtgcga gaggacgtgc ggccgccgct gcggaccctg
    24181 accgacgagg agcgggccga ggtggccgcc gccgtggagg gggcggctac gccggcgggt
    24241 tgacgaggag gtcgctccgc tcgccgaacc gccgcaggaa cctggcgtgg ttgcgcctgg
    24301 agcgccggcc acgctccgcg gggtcgaggg ccgcccaccc tcggtgctcg tgccgccgga
    24361 tcggcacgtc gatgcggacg gcccggagcc ccagggcctt cacctcgaag ctgaggtcga
    24421 cgtccgccca ccggtagaag cggtagccgg gatcgaagga cactcgggcc agaaggtcgc
    24481 gccgcagggc catcaggtag ccctggatcg cgtcgacgtc cggggcgatg gactcctcga
    24541 aggagcggag gtctcgcgtg acagcgccca cgggcccgca gaccccgatc gtctcgtcgg
    24601 cgagtgcccg ctcgatcgga gcgtagggat cgcccacggc ctctaccgag gcgtcggcca
    24661 cgatcgcgat ccgccccatc gtctgccgga ggccggccgc cctcgccgac ccgaacccgg
    24721 gatcgcggtc gagcggcagg acccggacgc cctcgggcca ggtgcccggc ggggcgggat
    24781 cggcctccac gacgaggtgc tcgacccgcc gatcgccctc gtagcggcgg aacgaggcca
    24841 cgccccggag cacgtcgtcc caccaccgct ccgccagcca gtggacggtc aggtcgtggg
    24901 tggggggctc ctccaggcgc gacggaacgg cctcgggggg cacgcgcggc gcgggaccgg
    24961 ccggcgacag gtcgtagccg tccggcgcgt cgcggacctc ccagcccagc gccaccaggc
    25021 gctcgcggag cgcgtcggcc cggtcgaagt cccgcgccgc gcgtgcctcg gcccgctcct
    25081 ccgccagccg gcggacctcg cccggcggct cgctcacccg tggggggtga agtccgagag
    25141 gatccgccgg tagttctcca cgatcgccga gcgatccggg cccacccagg ccccgcccag
    25201 gacgatccga tccacgaggc cgtcgtaggc ggcgagctgc tcgcgacagg cctcgggcgg
    25261 cccgcagatc gcatacgtgt ccaccatcga catggcgagg tcggccatcc gctcgagatc
    25321 gcgagcggcg tgagcctccc ggagcggcgc cgacagctcc tcgaacccgt ggagggccag
    25381 caccggcttg tacgtccggg tcgtggcgta gaaggcgacc tggtacgcgg cctgacggcg
    25441 agccgtctcg acgtcgtcgt ggacctgcat gatcacgctc gtggtgaggt cgacctcgct
    25501 cggatcgcgc ccggcggcgg ccgctccccg ctccacggcc ggcctgacca cctcggccac
    25561 gtaggccggc gacgtcatcg ggtgccccag gagcccgtcg tagacgcggc ccgccgtctc
    25621 gatcatcttc tcgttgaccc ccgcgaggta gagggggatg cgaggtacgg acgaggcgcc
    25681 ggggaaggga ggcatcgtga tctcgtagaa gcggccgcgg tgatcgatcg gctccccgtc
    25741 aaaggtggcg agcaccccgc gcacgacctc cgcgtactcg gccatcttcg gcgcggggtg
    25801 ctcgaacggc accccgaacc gctggacgtt cacccgcttc acctgcgagc cgagaccgag
    25861 cacgaagcga ccccccgaca gctcctgaag atcgcgggcc gtggaggcca cgacggtggg
    25921 gctccggggg aaggcgagcg cgatcgcggt accgatcgtg accctcgagg tcgactgcgc
    25981 ggccaccgcg gcctggacga acggcgtatg cgaggtctcc gccacccaga cgctctcgta
    26041 gccgccggcc tcagcggcct tcgccaggtc cgcgatgagg gacatgtgcc ccccgacgac
    26101 gatgccgatg tcgggcacgg gcggcagtct acccgccgca gggggacacc gccgttcagc
    26161 ccagctcgtc ggaacggcga cccgccgaac tcccggtcgt gctcctcgat gtgccggggc
    26221 cttgcacccg gcttccacgc cccggtagcc tgcgaccgtg ccgacgctct acgagtggct
    26281 gaagttcgct cacgtgctgc tggccatcat cgccgtcggc ttcaacgcct cgtacgcgat
    26341 ctggctggcc cgagcggcga ggcacccgga gcgcgaatcg ttcgccctgc gcacgatcaa
    26401 gacgctggac gaccggttcg ccaaccccgc ctacgggctc ctgctcgtca ccgggctcct
    26461 gatgatctgg aacgccgaca tcccgatctc caccttctgg atctcgacct cgatcgggat
    26521 ctacgtcggg gttctcctcc tcggcctcgg ggtgtacacc ccgaccctcc gcaagcagat
    26581 ccgcgagctg gaggacgacg gtccgcgctc ccccgcctat cagcgcctgt cgacccgagg
    26641 gacggtggtc gggatcctcc tgggagtgat ggtcgtcgtg atcgtcttcc tgatggtgac
    26701 gaagccgacg ctctgaccga gtggacgcgc gggcggcggc ccaggagctc gagcggcgcc
    26761 tcggagcggt agcccgggcc gaacgggccg agcaggaacg acgctacctg aagagctcgc
    26821 tgcgccacct cggcgtcagc gtcccggcga tccgccggac ggccttgggc ttcctcgagg
    26881 aacacgagct cgatcggtcc gagctcctgg cgcttgcccg ggccctgtgg aaggagccgg
    26941 tccacgagcg gaggatggcg gcggtcgaga tcctcgccga gcgggccgac ctgctgcggc
    27001 ccgatgacct tccctttgtc gaggagctgc tccgcgagtc ccgtacgtgg gccctcgtgg
    27061 atcccctcgc gatccaggtg gccggctcgc tcgtcgatcg gtggcccgag ctcggctcga
    27121 ccctggatcg gtgggccggc gacgacgact tctggatccg tcggtcggcc ctgctcgcgc
    27181 tgctgcccgg cctgcgagcc ggcgcgggga gccttaaccg cttcctgggc tacgctgacg
    27241 ccatgctcga ggagcgcgag ttcttcatcc ggaaggcgat cgggtgggtg ctgcgcgaga
    27301 cgggcaagcg tcgcccgggc gtcgtggccc ggtggctcgg gccgcgagcg ggccgagcat
    27361 cgggcgtgac cctgcgcgag gcggtcaagc atctgtccga ggggcagcgg gaacggatcc
    27421 tgcgagccgc cggacgctga ggaggaggcg ccatgatgag gaaccgggtg ccggccctcg
    27481 ccctgctcgt cgccctcgtg gcggcgggat gcgggggcgg cgaagaaacg gtggaggtca
    27541 ccctccagga gttcgcggtg ctgcccgccc aggacaccgt ctccgccggg tcggtgacct
    27601 tcgacgccac gaacgagggg cccgacgacc ctcacgagct cgtggtcatc caaaccgacc
    27661 tcgcccccga ggagctcccg accaccgatg agggcggggt cgacgaggag ggcgagggca
    27721 tcgaggtcat cggagagatc gaggagttcc ccgtcgggga gaccgagtcg gcgaccttcg
    27781 acctcgacgc gggcgactac gtgctcatct gcaacattgt cgaggaggag gacggcgagc
    27841 tcgaggctca ctacgagctg gggatgcggg tcggcttcac cgtggagtga gcggggttca
    27901 gcgcgggggt gcggcgcccg gctcctcgtc gtagacgact acggagcgga tcgggtggcc
    27961 ctcgcgcgtc tcgagagcgc gtaggccctc ggagaaggcc tcgagcggcc accggtgggt
    28021 gaccgagcgg cccaggtcga ggtcgccccg ctcgaccatc gccagcagct cctcgaggtc
    28081 gcggggagac gacccgaacg agccaaccac ctcgagctcc tgcgagacga gcagggccga
    28141 ggggaggagg tcgagcggag ccggcgtgag gcccacgatc gtggcccggc cccccgggcg
    28201 gagggacttc accgcctggc tctgcgaggc ggggctcccc acgaactcga atgctcgatc
    28261 caccccgccg ccggagcggc cgcggatctc gcgggccggc ttgccgtcgt cggcgtcgat
    28321 cgtctcgtcc gctccccacg ccagggctcg ctccaggttg gccgggtcga ggtccacgcc
    28381 cgccacccat gaggccccca gagctcgcgc cagcaggagc gcgtgcatcc ccagacctcc
    28441 caggccgaag atcgcgcacg acatccccgg ctcgaacccc gagcgccgca tcgcgtggaa
    28501 cggggtggcc accgcgtcgg tcaggatggc cgcctgctcg agggggatcc cccccggcac
    28561 cggcaggagg tcccgatcgc gggccaccgc gtactcggcc tgcgagccgt cgccgtcgat
    28621 ccccgggatc tccagccgcg cgcagagcga ttcgcgaccg gccgcgcagg cggggcactc
    28681 gccgcaggcc ctggccatca gcaccgccac gcggtccccg accgaccacg acgtcacgcc
    28741 ctcaccgagc atctccacca caccggcggc ctcgtgcccc aggatgaggg gcgtcggacc
    28801 gcccggcgtg gtcccgtcca caacgtggag gtcgctggcg cagatgccgc aggcggcgat
    28861 ccgcaccagg acctcgcccg gtgccggctc ggggaccggg acctcgtcca ccgagatcag
    28921 ctcgcgctcg tcgtcggacc ggacgctgtg caggcgggcc gcccgcatcg tggtcggtac
    28981 gctcatcggc gccagcctac ccgccctccc gtgacgggta cagtgggggc gtgcagcgga
    29041 tcgtcgagac catcgtgctg accgaggagg acctccgggt cctgggcgag accgacttcg
    29101 gcgtcccggg cctgatcgcc cacgagtgga tcggcccctt cgtcgaggtg caggccgtgg
    29161 ggccgctcat catggtccac gacgggatct tcgatccccg gaagggcatc acgcaccacc
    29221 cccaccggct gaacgagcgg ctcttctaca tcctcgaggg cacggtggac cacgacgatg
    29281 cgctgaacgg catcaccggc cacatgggca ccggcgacct ggggcggctc acggagggac
    29341 gccgcgggat gttccaccag gagtggaaca acaccgacgg gcgagcccgg gccttcatcc
    29401 tcgtctacgg gaccgacccc gtgcccccga ccgcctcctt ccaggccctg cgcgacgccg
    29461 acgcccctcg ctaccaggcg ttcccgggcg tggagtccaa ggagatcgtg ggcccgaagg
    29521 ccgacctgcc gctgcacggc gacgtccgtc tgtggaccga cgaccggatg gagcaggagt
    29581 ccgagagcgc gctggagctc gaggagggcg aaggcggcct ggtgttcccg atcgaggggg
    29641 ccgtggtcgt ggacgggtcg gagctccggc ccgaggcgat ggcggtcctt cccccgggcg
    29701 ggtcctcgcg gagcgttgcg gtctcgtcgc cggagggagc tcgcgtgctg cgcgtcgtgt
    29761 tcgggcccgg ctacggcctg gccctgcgtt ccgcgaccta gacgatcagg ttccgatcgt
    29821 cgagcgggta gtccacgcgg atcacctcac agtcgcgtga gaacacccac tcgctgaacg
    29881 acgtgtgcgc gggctgcgaa tagtaggcct ggtgggcctc ctcgctctcg aactcggtga
    29941 gcagcaggta ctcgtagccc tcgttgcggc cgctgacgtc cctgccgagc cgcaggcccg
    30001 tgaatccggg gatcgtctcg ggccacgccc ggacccgatc gaacatctcc cgctcctcgt
    30061 cctccgacag cggacgagcg aacttgaaca acaccacgtg ctgcgctcgc acggctccac
    30121 ctccaggccg catggtagcg tgcggccctg gaggtgagca tgagggcgac gaccgactgg
    30181 gggttcgcgc gcaggacgcg cgtcgatgtg ggcgagggtc tggcgtcgat cctcgcgctg
    30241 tcgggcgcca ccgacctcat ctcgttcgcg gggggcttcc ccgaccccga gaccttcccc
    30301 accgacgagc tcgccgcgct ggtgcagcga gtgtcccagg accccgcggc actccagtac
    30361 gcgccgacgc cgggcctgcc gggtccgcgg gccttcctgg cggaccggct cgagacgcgc
    30421 gagggccagc gcccgagcga cgaggagctg ctcatcacga gcggcgggat cgaggccctc
    30481 gagctgctcg ggaagagcct gatcgacccc ggggacctcg tgctcgtcga agggccgacc
    30541 tacctcggag cgatcatggc cttccgcagc ttcgaggcgg acgtggtggc ggttccgatg
    30601 gacgacgacg ggctcgacgt ggggcggctc gaggcgacgg tccgggagcg cggccggccg
    30661 aagctcctgt acacgatccc cgaccaccag aaccccgcgg gggtcagcct ggccgaggag
    30721 cggcgttccg cgctggtgga ggtggcgcgg cgccacgggt tcctcgtggt ggaggacgtc
    30781 gcataccggg agctcgggtt cacggaggat cgccccccga gcctctgggc gctgggaccc
    30841 gacgtggtcg tgcaggccgg gacgttctcc aagaccttct tccctggggt gcggctgggc
    30901 tgggcggccg gcccggccga ggtcgtggcc cagatggtga acgcgaagca gaacaccgac
    30961 cagtgtgcgg gcgcgctcgg ccagcggctg ctcgaggagt acggccgccg cggcctcctc
    31021 gacgagcaga tcgaccgggc gcgcggcatc taccgctcgc ggcgagacac cgcgatgagc
    31081 gctctcgagc gccacgtcgg cgaccgcgcc acctggacgc acccgcacgg cggcttcttc
    31141 acctggctgg agctggccga ggacggcgcg gatgtcgcga ggcgtgcgct ccaggcgggc
    31201 gtcgcggtcg tccccgggag gccgttcttc agcggcgtgg gcggggagcg caacatccgc
    31261 atctcctaca gccaggcctc ggaggagcgg ctcgaggagg gaatcgcttt gctcggcccg
    31321 atcgtcaggg cacgggcctg acgaggatca cggcccgttc gtcccagccc tcgagcgcga
    31381 gctcgcgcag ggggaggaag ccaagcgctc tgtagaacgc cctggtgtcg gcgtagttgt
    31441 cggtctcccg ccagtcgggc tcggactcca ccacggtcag gaccgagaga aactgtactc
    31501 cggcgcccgc gagatcgctc acacagcgct cgaccag
//
LOCUS       DASUTZ010000049         2725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_142340, whole genome shotgun sequence.
ACCESSION   DASUTZ010000049 DASUTZ010000000
VERSION     DASUTZ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142340"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VF097_02370"
     CDS             complement(<1..785)
                     /locus_tag="VF097_02370"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX6261673.1"
                     /translation="MRVCLMVEGQEGVTWEHWLALARACEEHGLEGLFRSDHYLSVED
                     DETRGSLDAWTTLAALAAETERIRLGTLVSPVTFRHPSLLAKAAVTADHISGGRVELG
                     IGAGWHLREHEAYGFSFPEPPERLDLLAEQIEIVHRQWSEQMVSFQGKHYRLEAVKAL
                     PKPVQDPHPPLIMGGHAKRRSVELSARWADEYNVFFSTPEECRAKRARVEEAWRVAGR
                     EGLVFSLMTPCVVGRDEGEVEERVAAVQRMEGEEPDPVAYRSA"
     gene            complement(862..1185)
                     /locus_tag="VF097_02375"
     CDS             complement(862..1185)
                     /locus_tag="VF097_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013602013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261674.1"
                     /translation="MRRMLVASIVAAFVVGASGIAQADHTHVRILPTGDCVILAADGN
                     EKYVVLPDENPHVDAGLPENRKHPLHVLVHLGQPGSDGSIAVMGSAQDPCSEAGGQTG
                     SYVNP"
     gene            complement(1256..>2725)
                     /locus_tag="VF097_02380"
     CDS             complement(1256..>2725)
                     /locus_tag="VF097_02380"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX6261675.1"
                     /translation="AASAFAGPGILALGLGALLWRLVPRRPLGSVLGLAVLGWGIFGP
                     TLAPDVFGEADIPVFVIQGVLLTASAVAVLGFNQESVGHAVRAVAGGARNLAARLGLA
                     YPLAKRFRTVMTLSMYALVVFTLVFISVLSNIFGGQVEDFTRQEAGGYDLLVRSSAAN
                     PLTAEAAAAEDGVEAAAPIRYAAFSVEFSTELQPEAEPWALSGIEEGFLAQGPPALEK
                     LAPEYGSEREAWEALLADPSLIILDGFFLQQGGPEAPPADLGDAVTIRDPLTGTEVER
                     TVIGVTRGGFSFSGPFVSEESILELAGPRAVATRLYLTLEPGADPVEVATRLEEEHIP
                     SGVEARTFRAIVSESQSVNLQFIRLMQGYLALGLVVGISGLGVIMVRAVRERRREIGI
                     LRSLGFQSRTVGRSFLLESAFVALEGLLVGAALAIVTSYQLVENAEVFGDLDVTFVIP
                     WGEIAIVLAATLVASLLATGWPARQASRIRPAVALRVAD"
BASE COUNT          426 a         1020 c          925 g          354 t
ORIGIN      
        1 ttggccgagc ggtaggccac ggggtcgggc tcctccccct ccatgcgctg gacggccgcc
       61 actcgctcct ccacctcgcc ctcgtcccgg cccaccacgc acggcgtcat cagggagaag
      121 acgagcccct cccggcccgc caccctccac gcctcctcga cgcgcgcccg cttcgcccgg
      181 cactcctcgg gcgtcgagaa gaacacgttg tactcgtcgg cccaccgggc ggagagctcg
      241 accgagcgcc gtttcgcgtg gcctcccatg atcagcggcg ggtgcgggtc ctgcaccggc
      301 ttcggcaacg ccttgacggc ctcgagccgg tagtgcttcc cctggaacga gaccatctgt
      361 tcgctccatt gccggtggac gatctcgatc tgctcggcca gcaggtccag ccgctcgggg
      421 ggctccggga aggaaaaccc gtacgcctcg tgctcccgca ggtgccaccc ggccccgatc
      481 cccagctcga ccctcccccc cgagatatgg tccgccgtca cggccgcctt cgccagcagc
      541 gacggatggc ggaaggtcac aggggagacg agcgtgccga gccggatccg ttcggtctcg
      601 gcggccagcg ctgccagggt cgtccaggcg tcgagcgatc cccgcgtctc gtcgtcctcc
      661 acggagaggt agtgatcgga gcggaagagc ccctccaggc cgtgctcctc gcaggcccga
      721 gcgagcgcca gccaatgctc ccaggtgacg ccctcctggc cctcgaccat cagacagacg
      781 cgcacgaagg tttcctacca cgaacgtcga gggcccggcc gcgctgcgcg cggccgggcc
      841 ctcgacacac ggatcccgtc gctaggggtt gacgtagctg ccggtctgtc cacccgcctc
      901 gctgcagggg tcctgggcgg aacccatgac ggcgatcgag ccgtccgagc cgggctggcc
      961 gagatgcacc aggacgtgta ggggatgctt ccggttctcg gggagcccgg cgtcgacgtg
     1021 cgggttctcg tcgggcagca cgacgtactt ctcgttgccg tcggcggcca ggatcacgca
     1081 gtcgccggtc ggcaggatcc taacgtgcgt gtggtcggcc tgggcgatcc cagacgcgcc
     1141 gaccacgaac gccgccacga tggaggcgac gagcatccgt cgcatggatg atccctcctt
     1201 ccggttcgcg gacactgctc gtacctctac cccgagaggg tgtcccgaga caagttcagt
     1261 ccgcgacgcg gagggccacc gccggacgga tccggctggc ctgccgcgca ggccaccccg
     1321 tcgccagcag cgacgccacg agcgtggcgg ccaggacgat cgcgatctcg ccccacggga
     1381 tcacgaaggt gacgtcgagg tccccgaaga cctccgcgtt ctccacgagc tggtaggagg
     1441 tgacgatcgc cagggcggcc ccgaccagca agccttccag cgcgacgaag gccgactcga
     1501 ggaggaacga gcgccccacc gtcctggact ggaacccgag gctgcggagg atcccgatct
     1561 ctcggcgtcg ctccctaacc gcgcgcacca tgatcacgcc cagccccgag atccccacga
     1621 ccaggccgag cgccaggtag ccctgcatca gccggatgaa ctgcaggttc accgactggg
     1681 actcggagac gatggcccgg aacgtcctgg cctccacccc gctcgggatg tgttcctcct
     1741 cgagccgcgt ggccacctcc acggggtcgg cgccgggctc gagcgtcagg tagaggcggg
     1801 tggccacggc ccgggggccg gcgagctcca ggatgctctc ctccgacacg aaggggcccg
     1861 agaacgagaa gccgccccgc gtcacgccga tcaccgtgcg ctcgacctcc gtgcccgtca
     1921 ggggatcgcg gatcgtgacg gcgtcgccga gatcggcggg cggagcctcg ggccctccct
     1981 gctggaggaa gaacccgtcc aggatgatca gcgacggatc ggcgagcagg gcctcccagg
     2041 cctcgcgctc gctcccgtac tcgggggcga gcttctccag cgccggggga ccctgggcca
     2101 ggaagccctc ctcgatcccc gagagcgccc acggctcggc ctcgggctgg agctccgtgc
     2161 tgaactccac gctgaaggcc gcgtagcgga tgggggccgc ggcctccacg ccgtcctcgg
     2221 ctgcggccgc ctcggccgtc agggggttcg ccgccgacga ccggaccagc aggtcgtagc
     2281 cgcccgcctc ctgccgggtg aagtcctcga cctggcctcc gaagatgttc gacagcaccg
     2341 agatgaagac gagagtgaac acgacgagcg cgtacatcga gagggtcatc acggtccgga
     2401 accgcttggc cagcgggtag gcgagcccca gccgcgcggc gaggttgcgg gcgcccccgg
     2461 ccaccgcgcg gaccgcgtgg ccaaccgatt cctggttgaa gccgaggacc gccacggcgg
     2521 acgccgtcag cagcacgccc tggatcacga acacggggat gtcggcctcc ccgaagacgt
     2581 cgggagccag cgtgggcccg aagatccccc agcccaggac cgccagcccc aggaccgagc
     2641 ccagcggccg tcgcgggacg agccgccaga gcagggcacc cagccccagg gcgaggatgc
     2701 ccgggccggc gaaggccgag gccgc
//
LOCUS       DASUTZ010000050        15816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1309125, whole genome shotgun sequence.
ACCESSION   DASUTZ010000050 DASUTZ010000000
VERSION     DASUTZ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 15816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15816
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309125"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2564
                     /locus_tag="VF097_02385"
     CDS             <1..2564
                     /locus_tag="VF097_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX6261676.1"
                     /translation="MRLLEWFVAERRAGNPEVKQLLETRELWFVPVVNPDGYEYTFTD
                     DRLWRKNLRDNNGDGVITPGDGVDLNRNYPEHWNYDEEGSSSQFASETYRGPAPASEP
                     EVQAGVRLFDMLPKLRFAISYHSFGQLLLYTQGWQVQTPSADDPIYVALTGTDDDPAI
                     PDFDPGVGADLYTTNGEFTDWAHGERDVLAWTPELSEGCPGCGFVFPDDEDLVQEEFE
                     KNLEFALNVARSALDPDDPASHMDLDTEDFYLDVSEIDPWKSNNPSSDLKVDVSYAGE
                     SSQPVEVLAKRGLGAVTLHYSVNGGPAQTASTSPSPDGEVFGGNNAYDTYYHYLRGEI
                     PGLSVGDSVEYWFSADSSSSDHQTFEVVEDADADVLIVANEDRTGAVNIPGYASTDPN
                     VPNYLSSYTDALDAAGHSYDVWDVDAMGREAPDHLGVLSHYDAAIWYMGNNFVTRTAG
                     RGPGNIDRLANDLILESRAYLNEGGKLLYTGQWAGAAENGLAGTQLYDPVAGEACVVG
                     GVLVLDRCQVFSDKNDFLQYYLGAYIYNSDAGTDGAGNPFQVLGVDDPYTGTNWEFNG
                     ADSAQNQVHTASFITASSLLPPDEYPQFASTGPAVWDTGAAGAFEPFDGDWYVYSNMG
                     DVSYKRLMRTIDLTGVSPADNPTLTFRFSYDTEPDWDFVFVEAHTVGVDDWTTLPDQN
                     GNTNNNTGSSCASGWFELHPWLERYQGSDCSGSNSDTGGEWNAHSGRSAGWEEWEIDL
                     SAYAGSEVEVSISYASDWAVQGLGAWVDHVGVSTEAGVESFETDLGAWTVAGPPEGSD
                     PNPNDWERTQSVGFEEGAVVATDDTLYFGFGFEGITGASSRAAVMEKSMGYLLGP"
     gene            complement(2670..3137)
                     /locus_tag="VF097_02390"
     CDS             complement(2670..3137)
                     /locus_tag="VF097_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6261677.1"
                     /translation="MAEHVTLRDVTDEDLDVFYQHQLDPEAAGMAAATPRGRGAFMAH
                     WRRILADPTVVTKAVLVDGELAGNVVSFLREEKREVGYWIGKRYWGRGVATTALAWFL
                     ELIDERPLHAYVATHNLASVRVLEKCGFEVVGEDPAFTTIAGEPVAGLILRLG"
     gene            3216..4253
                     /locus_tag="VF097_02395"
     CDS             3216..4253
                     /locus_tag="VF097_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261678.1"
                     /translation="MRKHIQKILVGALAVLVVAVAPAGAHLNKKLKHLIKHLNPVFLN
                     QNEKAADAQLLDGIDSAEFLRADATAADSDLLDGMDSSAFLPAGGTAADSDLLDGMDS
                     GAFQRSFVRTVIVDTAAELIAAVGSASPNTMIQLEPGTFNVGTTMLSVPSGVTLQGAG
                     IRTLIVGAGSADPNHAVLAAGGNTEIRSLVILNTGNQPFAVAARIQANARLFDVNAQA
                     QNGTTESVAIDAVTGGGSVVPLLRQVGAFTLGAPSAEAIAMRAAGVNVHVVDSILQAF
                     QGSGGTGTAFRGIGGLHRIERSILFGDTAAVNVTNGGQARVALTRLEGTIFGAVCFDV
                     YDDDFAPVTCA"
     gene            4388..7246
                     /locus_tag="VF097_02400"
     CDS             4388..7246
                     /locus_tag="VF097_02400"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEX6261679.1"
                     /translation="MRRAATLLALLALAAATTAVSPLAAAVQPEEEIQPLYKQPPGLG
                     AHEAIASVSAPTLAAFRAEREAARSAALAGDVPAGPNVRTTNSLGTQAEISMAATGGG
                     GQNIVITYNGGPNGLGFVSSQDGGRNFQAEQSPPTPAGSNPCCDPSVVADAVSSFFLI
                     QLFRDDGANAPAAGNCTNSLHVSTDGGQTFSNIVSSPFSYPPGGPDFPDQPHIGIDRG
                     NPNNLYVFTRHFTSGINCPQTGGSGTVQGEVVCSNDGGATWSNPFVFPQFTDTAHLDV
                     APDGTAYVVGNGVGTNMGTTRVILWRATATTCPGAGNTPNFAGPTVVADNLTFGNVGI
                     DREFPQPTVLADPTNSNRVYVNFSADSTQGSGDREALLSRCDFPAATCTALLTVNDNP
                     ADGSDQYFPMACIDLATNNVFVSWNDDRSGQEEIRATTVTNNGTTTGASRRVSEVTWP
                     IINPGGTPDYGDYNENNGACRANHHYAAWSSQVSPPGITPASTDIDVFFTVVNEPPEA
                     DAGGPYMTVEGTDVGLSGSATDPENDTPFTFEWDLDGDGEFDDATGQTPTFTDVGQDG
                     VFPVCLRVTDSIGDAGEDCTTVSVANVAPSLVGLSQDGPKPEGDAVTVSGTVTDPGWE
                     EMLSVTIDWGDGTPVETVGGILENERPDATLTFSAPHVYGDNGTFPVEVCGHDDDTQT
                     CQMIDVIFTNVPPTAGFDLTGTEIVNGVPTVIGEAGEPVELTGRATDPGSDDLFLRWD
                     FGDGSPIVLVTSLVNPPLPDPPNSPSVQPRDVTDTQSHTYGDACIYEVTFSATDDDGG
                     ASGPATLAVLIAGNADRTRPTGWWHRQYMRDGARSDFDVATLECYLAIARFGSAVFDE
                     ATALASLAHAADVLKGGGKPQHLSRFDRELLTAWLNLANGAVGFTELVHDADGDGTLD
                     TTFGAAVAAAESVRLGASTPAQVRAQQRILHRINVPAS"
     gene            complement(7299..7655)
                     /locus_tag="VF097_02405"
     CDS             complement(7299..7655)
                     /locus_tag="VF097_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX6261680.1"
                     /translation="MAIEDLAAEQFCYVTSIGRVTGRPHTIEIWFALDGRTLYLLSGG
                     GRASDWVRNLERTPEATVRLGDRTFPGRARVVTDAEEDERARRLVHDKYQPGYGGDLA
                     GWRRRSLPVAIDLHED"
     gene            7722..9203
                     /locus_tag="VF097_02410"
     CDS             7722..9203
                     /locus_tag="VF097_02410"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6261681.1"
                     /translation="MACDDEDLGLPIKLGPCSNAEYDPEPLPPVLREAIRRTRASSER
                     HARRLAISRRDFLRSSMAAAAMLFALDACTRDRMRAVERRDPGGRYRIPDEAPEDAPL
                     AEDTVEGEEFVFDVQGHLLEYDLDPATRWEPFWGDGFPQVACGEDDPRACFSMEHFME
                     EFFLRSDTSMVALAGLPIAPAGSPLSPEIMEEARRVAEGLCRDERVLLHALVLPNVGP
                     LAANLDAMEEGVGRYPIAAWKVFTHFPDVYEGSGSGWRLDDGDPSLATVGTPFIRKAV
                     ELGVPRIAVHKGLSGGSALASPIDVGAAARAHPDVAFIVYHSGFETGISEGPFTRRTA
                     GGGVNRLIASLKRNGIRPGGNVYAELGSTWWYVMRYPDQAAHVLGKLLKHLGEDNVLW
                     GTDSIWYGSPQDQIQALRSFHISEEYQERFGYPALTKRIKRKILGLNAADLYGIDPTS
                     VPCTFTRPELRRIRRQLRRPDRTNGPSTSAEARRVREHHRGMP"
     gene            complement(9225..10865)
                     /locus_tag="VF097_02415"
     CDS             complement(9225..10865)
                     /locus_tag="VF097_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6261682.1"
                     /translation="MRLAEAIGLALAKLEVRDAFGLLGSGNFLVTQAMVDRGVRFVAA
                     RHESAAVSMADGYARVSGRPGVVTVHQGPGLTNAMTALTEAAKARTPLMVLAADTSAG
                     AGRSNFAVDQAGLATAVGAVAERARSPETALEDLARAWRTAVVGRRAVLFSMPLDVQA
                     GEAAGDEIEAPEAPEPAAPAHEDVERIADLLAAAASPVLVGGRGAVLAGAGPAIEALG
                     NRVGGLLATSANGHGLFAGNPWSLGISGGFASPTAGRLIREADVVVSFGASLNMWTTR
                     HGELVGPGARVAQVDRDADELGAHRPIDLGVVGDARLTAEAVAAELDRRGHRVPGRRS
                     DDVAERIAGGRWADEPHEDASKDGRLDPRTFSKALEEALPPERVVAVDSGHFMGWPPM
                     YLSVPDPAGFVFTQAFQSIGLGLSSAIGAAVARPDRLTVACLGDGGAMMAAGEFETLV
                     RLGLPLVVVIYNDSAYGAEVHHFGPQGYPVDLVRYPDPDLAGMARGLGAEAATVRSLD
                     DMSVVDGWLERRDGPLVLDVKIAPDVVAEWLEEAFRSH"
     gene            complement(11090..11785)
                     /locus_tag="VF097_02420"
     CDS             complement(11090..11785)
                     /locus_tag="VF097_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HEX6261683.1"
                     /translation="MAHERVDARTEEEIRADERRRVLDSDRDNDGVDDRVERREVHDD
                     DHVHERPAYGPGYVRGGVSFGSIVTGTAVAFGAMSLLTMLIAGIAVAVGVTAEGVDAS
                     ATEIGWGVGIALVVTQFIAYFWGGYTAGRMARGAGLLNGLLVPVLALLLMGLAVGLVA
                     AFGGEARFAVPFTDFRVPVEEDVVLTWGIGLGITTLVVMFLGGILGGMFGARWHDKLE
                     AVAGDRYDYRRAS"
     gene            11968..12561
                     /locus_tag="VF097_02425"
     CDS             11968..12561
                     /locus_tag="VF097_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261684.1"
                     /translation="MRTLRTPIPFVLLVAVLATTACASDPAPFRAAAPTAAPTASSGA
                     TPAAAPQPGSPRSQIYAAVIRQLVTVDHTFGGAKPPFDRVLVVDGVFPEPKRAPWGAA
                     SPRHPFPEPLKTAIEGHLEDLPPLCFIGEEQAPRDLPTAQAAVIRLGPIVQDWEHHRV
                     PASLECGSVCGIWLSYILADEDGRWVIIDRSGPLAIA"
     gene            12601..13806
                     /gene="lhgO"
                     /locus_tag="VF097_02430"
     CDS             12601..13806
                     /gene="lhgO"
                     /locus_tag="VF097_02430"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HEX6261685.1"
                     /translation="MERFDVAVIGGGLLGLATARHLLERRPDLRLAVLEKESALALHQ
                     SGRNSGVVHSGIYYKPGSLKARLCREGRSMIERFAADHDIPYEACGKLIVAVEESELG
                     RLAELARRAGANGVEIEEVGPERIRELEPHAVGIRGLHSPGTAITDFRRVALALGEEV
                     GRMGGTILLEHGVTSIRGRGERRRLETRRGPVGARFVVSCAGLQSDRVAALTGDRGGQ
                     RIVPFRGAYQRLGARAAGLVQGLVYPVPDPRLPFLGVHFTRRVDGEVWVGPSAVLALA
                     REGSRPWQVNLRDVASTLRFGGFWRMAARHWRTGLEEVTLDLSKRAFLKACRRYLPEL
                     ERQDILPGPFGVRAQAVNRDGSLEDDFSLPESHGVLHVRNAPSPGGTASLAIGRTLGE
                     RVLARLDER"
     gene            complement(13820..14698)
                     /locus_tag="VF097_02435"
     CDS             complement(13820..14698)
                     /locus_tag="VF097_02435"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6261686.1"
                     /translation="MSGRVAYLGMGRMGAAMAATLRREGFDVVVWNRSPEKAEAVAEA
                     SGCEVAATAPEAVGSAEIVLSSLADDEAVRDVYLGSGAVAATLRPGTVVCEMSTIARE
                     TVLGLREPIEERGASLLDAPVSGSVSLAETGKLTIMVGGEEEALARARPVLEALSGKL
                     MHVGELGAGATMKLAVNAVVHALDVALSEALVLAERAGVDRPAAYDVFANSAVAAPFV
                     LYKQEAFLKPDETPVAFSLDLVAKDLDLIFDLARQVGARMDQGETNREIVRRALEAGL
                     AELDLSAIAVLLRDST"
     gene            complement(14695..15396)
                     /locus_tag="VF097_02440"
     CDS             complement(14695..15396)
                     /locus_tag="VF097_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018839527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6261687.1"
                     /translation="MADGSVVVIGGTSGLGREVAAHYAAAGRPVVISGRDEERCSAIA
                     EEIGGDTTAIGLDLHEPEKIGAALEGVGGVDHLVIASIYRDENRVADYDVQAAIRLVT
                     LKLVGYTEVVHALTSRLHDESSILLFGGLAKDKPYPGSTTVTTINAGVVGLVRTFVSE
                     LAPIRTNAIHPGIVADSPFWAAKPPEVLEAVREKTPTGRPLRMQDIVHASVFLLENPA
                     VNGVNLAVDGGWSLT"
     gene            complement(15389..>15816)
                     /locus_tag="VF097_02445"
     CDS             complement(15389..>15816)
                     /locus_tag="VF097_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003994430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6261688.1"
                     /translation="DKQTQDMLSVSASMRTALLVYSGITVKMMVDSDLGAQLSTMFMV
                     QYEPDGVAGPHDHPLEETYLIVEGEVDATFDGERYRMRPGDVAWAGVGCVHSFSNPGP
                     GIVRWLETQAPQPPARHSYRFARDWDYLKDALSKEAGRG"
BASE COUNT         2433 a         5790 c         5364 g         2229 t
ORIGIN      
        1 ccatgcgcct cctcgagtgg ttcgttgccg agcgtcgcgc gggcaacccc gaggtcaagc
       61 agctcctcga gacccgggag ctctggttcg tcccggtggt caacccggac ggctacgagt
      121 acacgttcac ggacgaccgc ctgtggcgga agaacctccg ggacaacaac ggcgacggcg
      181 tgatcacgcc gggcgacggg gtggacctga accggaacta ccctgagcac tggaactacg
      241 acgaggaggg gtcctcctcg cagttcgcca gcgagacgta tcgcggcccg gctcccgcat
      301 ctgagcccga ggtgcaggcg ggcgtcaggc tgttcgacat gctgccgaag cttcgcttcg
      361 cgatcagcta ccactcgttc ggacagctcc tcctctacac gcagggctgg caggtgcaga
      421 ccccctcggc cgacgatccc atctatgtcg ccctgacggg gaccgacgac gaccccgcga
      481 tcccggactt cgaccccggc gtcggcgcgg atctctacac gacgaacggt gagttcaccg
      541 actgggccca cggggagcgg gacgtgctcg cctggacccc cgagctgtcc gagggatgcc
      601 cgggctgcgg cttcgtgttc cccgacgacg aggaccttgt gcaggaagag ttcgagaaga
      661 acctcgagtt cgcgctgaac gtggcgcgct cggcgctcga cccggacgac ccggcctcgc
      721 acatggacct cgacaccgag gacttctacc tcgacgtctc ggagatcgac ccgtggaagt
      781 cgaacaaccc ttcgtcggac ctgaaggtcg acgtttcgta cgcgggtgag tcctcgcagc
      841 cggtggaggt cttggcgaag cggggcctcg gggccgtgac gctccactac agcgtcaacg
      901 gcgggccggc gcagaccgcg tcgacgagtc cgtcgcccga cggtgaggtg ttcggcggca
      961 acaacgccta cgacacctac taccactacc tccgcgggga gatccccggc ctgtcggtcg
     1021 gcgactccgt ggagtactgg ttctcggcgg actcgtcgtc gagcgaccac cagacgttcg
     1081 aggtggtcga ggacgcagac gcggacgtgc tgatcgtcgc caacgaggac cgcacggggg
     1141 ccgtcaacat ccccgggtac gccagcaccg acccgaacgt cccgaactac ctctcgtcgt
     1201 acacggacgc gctggacgcg gccggccaca gctacgacgt gtgggacgtg gacgcgatgg
     1261 gccgggaggc cccggatcac ctcggcgtgc tgagccacta cgacgcggcg atctggtaca
     1321 tggggaacaa cttcgtgacc cggacggccg gccgcggccc cggcaacatc gaccggctgg
     1381 ccaacgacct gatcctcgag tcgagggcct acctcaacga gggcggaaag ctcctgtaca
     1441 cgggccagtg ggcaggcgcg gcggagaatg gcctcgcggg cacccagctc tacgacccgg
     1501 tggccggcga ggcctgcgtg gtcggcgggg tgctggtgct ggatcggtgc caggtgttct
     1561 cggacaagaa cgacttcctc cagtattacc tgggcgccta catctacaac agcgacgcgg
     1621 gcaccgacgg ggccgggaac cccttccagg tgctgggggt ggacgatccg tacaccggga
     1681 cgaactggga gttcaatggg gcggacagcg cccagaacca ggttcacacg gcatcgttca
     1741 tcacggcgag cagcctgctg ccgcccgacg agtacccgca gttcgccagc acggggccgg
     1801 cggtgtggga tacgggagca gcgggcgcct tcgagccctt cgacggggac tggtacgtct
     1861 actcgaacat gggggacgtc agctacaagc gcctgatgag gacgatcgac ctcacgggcg
     1921 tgtcgccggc ggacaacccc acgctgacgt tccggttctc ctacgacacg gagccggact
     1981 gggacttcgt cttcgtggag gcgcacacgg tgggggtgga cgactggacc accctgccgg
     2041 accagaacgg caacaccaac aacaacaccg ggtcgagctg cgcgagcggc tggttcgagc
     2101 tgcacccgtg gctcgagcgg taccagggct cggactgctc cggctcgaac tccgacacgg
     2161 gcggtgagtg gaacgcgcac tccggccgct ccgccggctg ggaggagtgg gagatcgacc
     2221 tctccgccta cgccggcagc gaggtcgagg tctcgatctc ctacgcgagc gactgggccg
     2281 tccagggcct cggcgcgtgg gtcgaccacg tcggggtctc cacggaggcg ggcgtcgagt
     2341 ccttcgagac cgacctcggc gcctggaccg tcgcgggccc gcccgagggc agcgacccca
     2401 accccaacga ctgggagcgg acccagtcgg tcgggttcga ggagggcgca gtcgtcgcca
     2461 ccgacgacac gctctacttc gggttcgggt tcgagggcat caccggcgcc tcgtccaggg
     2521 cggccgtgat ggagaagagc atggggtacc tgctcgggcc gtagagccca gccggtacga
     2581 ggtgaggggc cggcggcccg ttcgggccgc cggcccctca ccctttctcg gcgagcgctc
     2641 tcgcccggcc tgtacgcctc tcgcgaggcc tatcccagcc gcagaatgag ccccgcgacc
     2701 ggctctccgg cgatcgtcgt gaacgccggg tcctcgccca cgacctcgaa gccgcacttc
     2761 tcgagcaccc tgaccgaggc gaggttgtgc gtggccacgt aagcatgcag gggccgctcg
     2821 tcgatcagct ccaggaacca ggccagggcc gtcgtggcca cccccctccc ccagtagcgc
     2881 ttcccgatcc agtagccgac ctcgcgcttc tcctctcgca ggaagctgac gacgttcccc
     2941 gcgagctccc cgtccacgag caccgccttc gtgaccacgg ttgggtcggc caggatccgt
     3001 cgccagtggg ccatgaacgc gccgcgcccc cggggcgtgg ccgccgccat cccggccgcc
     3061 tccgggtcca gctggtgctg gtagaagacg tccaggtcct cgtcggtcac gtcgcgcagg
     3121 gtgacgtgct cggccacggc gcatacccta ccgaccttca gccctcccga agcgcctgga
     3181 ggatgatgag cgccaccgat ccaagggagg aggagatgcg gaagcacatc cagaagatcc
     3241 tcgtgggcgc gctcgcggtc ctcgtggtag ccgtcgcccc ggcaggcgcc cacctcaaca
     3301 agaagctcaa gcacctgatc aagcacctca accccgtgtt cctgaaccag aacgagaagg
     3361 ccgcggacgc gcagctgctg gacgggatcg actccgccga gttcctccgg gccgacgcca
     3421 ccgcggcgga ctcggacctg ctggacggga tggattcgtc cgcgttcctc ccggcgggcg
     3481 ggacggccgc ggattccgat ctgctggacg ggatggattc cggcgcgttc cagcgctcgt
     3541 tcgtcaggac ggtgatcgtg gacacggccg ccgagctgat cgccgcggtg ggctcggcga
     3601 gccccaacac gatgatccag ctcgagcccg gcacgttcaa cgtgggcacg acgatgctct
     3661 ccgtcccgag cggcgtgacc ctccagggcg ccggcatccg cacgctgatc gtgggggccg
     3721 gttccgcgga tccgaaccat gccgtgttgg cggccggcgg caacaccgag atccgttccc
     3781 tggtgatcct gaacaccggc aatcagccgt tcgccgtggc ggctcgcatc caggcgaacg
     3841 ctcgtctctt cgacgtcaac gcgcaggccc agaacgggac gaccgagtcg gtggcgatcg
     3901 acgccgtcac cggaggaggt tcggtggtgc cgctgcttcg ccaggtgggc gccttcacgc
     3961 tcggcgctcc cagcgccgag gcgatcgcca tgcgggccgc cggcgtgaac gttcatgtgg
     4021 tcgactcgat cctccaggcc ttccagggat ccgggggcac ggggacggcc ttccgcggga
     4081 taggcgggct ccacaggatc gagcgttcga tcctcttcgg agacaccgcc gcggtcaacg
     4141 tgacgaacgg cggtcaggcg agggtcgcgc tcacgcgcct ggaggggacc atcttcggag
     4201 cggtctgctt cgacgtctac gacgacgact tcgcgccggt cacctgcgcg tgaggcggag
     4261 gggtgcatcc cccctcccac cgacggccga acggccctct tgacccgtgc gcggagcaag
     4321 cgtaggacgg gacccgggcc ggcgagcctc gtgcggacga ggccggcggc tcgtggaggg
     4381 aggtcccatg cgacgtgccg ccaccctgct cgctctcctc gcgctggcag ctgcgacgac
     4441 cgccgtgtcg ccgctcgcgg cggcggtgca gcccgaggag gagatccaac cactgtacaa
     4501 gcagcccccc ggcctcggtg cacacgaggc gatcgccagc gtgagcgccc cgaccctggc
     4561 cgccttccgg gcggagcgcg aggccgcccg ttcggcggcg ctcgcagggg acgtgccggc
     4621 gggacccaac gtccgaacga ccaactcgct tgggacgcag gccgagatct cgatggccgc
     4681 cacgggcggt ggtggccaga acatcgtcat cacctacaac ggtgggccga acggcctggg
     4741 cttcgtgtca tcccaggacg gcggtcggaa tttccaggcc gagcagagcc cgccgacgcc
     4801 ggcggggagc aacccgtgct gtgacccttc ggtggtggcc gacgccgtca gcagcttctt
     4861 cctgatccag ctcttccgcg acgacggagc gaacgccccg gcggcgggga actgcacgaa
     4921 ctctctgcac gtgtccacgg acggcgggca gacgttctcg aacatcgtca gctctccgtt
     4981 ctcgtacccc cccggaggcc cagacttccc ggatcagccc cacatcggga tcgaccgggg
     5041 gaaccccaac aacctctacg tgttcacgcg ccacttcacc tcggggatca actgccccca
     5101 gacgggcggg agcgggaccg tgcagggcga ggtggtttgc tcgaacgacg gcggagcgac
     5161 ctggagcaac ccgttcgtct tcccgcagtt caccgacacc gcccacctcg acgtggcgcc
     5221 cgacggcacc gcctacgtcg tgggcaacgg cgtcgggacg aacatgggaa cgacgagggt
     5281 gatcctgtgg cgagcgacgg caacgacgtg cccgggagcc ggtaacacgc cgaacttcgc
     5341 cggccccacg gtggtcgccg acaacctcac tttcggcaac gtggggatcg atcgggagtt
     5401 cccgcagccg acggtgctcg ccgacccgac gaacagcaac cgcgtctacg tgaacttctc
     5461 ggccgattcg acccagggca gcggcgaccg cgaggccctc ctctcgcggt gcgacttccc
     5521 cgcggcgacc tgcacggccc tgctcacggt gaacgacaac ccggccgacg ggtccgatca
     5581 gtacttcccc atggcctgca tcgacctcgc gacgaacaac gtgttcgtct cgtggaacga
     5641 cgaccgctcg ggccaggagg agatccgggc caccaccgtc acgaacaacg ggacgacgac
     5701 gggggccagc cgccgcgtct ccgaggtgac ctggcccatc atcaatcccg ggggaacgcc
     5761 cgactacggc gactacaacg agaacaacgg tgcgtgtcga gcgaaccacc actacgcggc
     5821 gtggagctcg caggtcagcc cgccgggcat cacgccggcc tccaccgaca tcgacgtgtt
     5881 cttcaccgtc gtcaacgagc caccggaggc ggacgcgggt ggtccctaca tgacggtcga
     5941 ggggacggac gtcggcctct ccgggagcgc cacggatccc gagaacgaca cgcccttcac
     6001 attcgagtgg gacctcgacg gcgacgggga gttcgatgac gcgaccgggc agacgccgac
     6061 ctttacggac gtgggccagg acggggtgtt ccctgtctgc ctccgggtga ccgactcgat
     6121 cggtgacgcc ggggaggact gcacgaccgt ctcggtcgcg aacgtggctc cctcgctggt
     6181 ggggctctcg caggacgggc cgaagcccga gggcgatgcg gtcacggtct cggggaccgt
     6241 caccgacccc ggctgggagg agatgctcag cgtcacgatc gactgggggg acggcacccc
     6301 ggtggagacg gtcgggggga tcctggagaa cgaacgaccc gacgcgacgc tgaccttctc
     6361 ggcgccccac gtgtacgggg acaacggcac cttcccggtg gaggtctgcg ggcacgacga
     6421 cgacacgcag acgtgccaga tgatcgacgt gatcttcacc aacgtgccgc ccacggccgg
     6481 cttcgacctc acggggacgg agatcgtgaa cggcgtcccg accgtgatcg gtgaagcggg
     6541 cgagccggtg gagctcaccg gacgagccac cgacccgggg tcagacgacc tcttcctgcg
     6601 gtgggacttc ggggacggat cgccgatcgt cctcgtcacc tcgctggtga acccgcccct
     6661 ccccgacccg ccgaacagcc cgtcggtcca gccccgcgac gtcaccgaca cccagagcca
     6721 cacgtacggg gacgcgtgca tctacgaggt cacgttctcg gcgacggacg acgacggagg
     6781 ggcgagcggg ccggccaccc tcgccgtcct catcgcgggg aacgcggacc ggacgcggcc
     6841 cacgggctgg tggcaccggc agtacatgcg ggatggcgcg cggtcggact tcgacgtggc
     6901 cacgctcgag tgctacctgg ccatcgcgcg gttcggcagc gcggtcttcg acgaggccac
     6961 ggcgctggcg agcctggccc acgccgcgga cgtcctcaag ggcggaggca agcctcagca
     7021 cctcagccga ttcgaccgag aactgctcac cgcctggctg aacctggcca acggcgccgt
     7081 gggcttcacc gagctggtcc acgacgcgga cggtgacggg acgctcgaca cgacgttcgg
     7141 ggccgcggtg gcggccgccg agagcgtccg gctgggcgcc tccaccccgg cccaggtcag
     7201 ggcccagcag cggatcctgc accgcatcaa cgtgccggct tcatgacggg gtgccggtgc
     7261 tcccgccccc tcccgggggg cgggagcacc ggcaccggtc agtcctcgtg gagatcgatc
     7321 gccaccggca gtgagcgtcg ccgccacccg gccaggtcgc ccccgtagcc cggctggtac
     7381 ttgtcgtgga cgagacggcg agctcgttcg tcctcctcgg cgtccgtgac cacccgcgcg
     7441 cggccgggga aggtcctgtc tcccaaccga accgtggcct cgggcgttcg ctccaggttc
     7501 cgcacccaat cagaggcccg cccgcccccc gacaggagat agagcgtcct cccgtccagc
     7561 gcgaaccaga tctcgatggt gtgcgggcgg cccgtgaccc gtccgatgga ggtgacgtag
     7621 cagaactgtt cggccgcgag atcctcgatc gccatctcga gcccacctta gccgcgtgaa
     7681 cctccccgca ctgtggagcg tctacctctc gggggacggc catggcgtgt gacgacgagg
     7741 acctcgggct gccgatcaag ctggggccgt gctcgaacgc cgagtacgac ccagagcccc
     7801 tgcccccggt cctgcgagag gcgatcaggc gcacccgggc ctcctctgag cgacacgccc
     7861 gtcgcctggc gatcagccgt cgcgacttcc tgcgctcctc gatggctgcg gccgcgatgc
     7921 tcttcgcgct cgatgcctgc acccgcgatc ggatgcgagc ggtcgagcga cgcgatccgg
     7981 gaggccgcta ccgcatcccc gacgaagccc ccgaggacgc gcccctcgcg gaggacacgg
     8041 tcgagggcga ggagttcgtc ttcgacgtcc agggtcacct gctcgagtac gacctcgacc
     8101 ccgccacgcg ctgggagccg ttctggggcg acggcttccc ccaagtagcc tgcggcgagg
     8161 acgacccgag agcctgcttc tccatggagc acttcatgga ggagttcttc ctcaggagcg
     8221 acacgagcat ggtcgcgctc gccggcctcc ccatcgcccc ggccgggagc cccctgtccc
     8281 cggagatcat ggaggaggct cgacgggtcg ccgagggcct ctgccgggac gagcgggtcc
     8341 tcctccacgc cctggtgctc ccgaacgtcg ggccgctcgc cgccaacctc gatgcgatgg
     8401 aggaaggtgt ggggcgctac cccatcgccg cttggaaggt gttcacgcac ttccccgacg
     8461 tctacgaggg ctcggggagc gggtggcgcc tcgacgacgg cgatccgagc ctggcgacgg
     8521 tggggacgcc gttcatcagg aaggccgtcg agctcggcgt gccccggatc gccgtgcaca
     8581 agggtctcag cggcggaagc gccctggcct cgccgatcga tgtgggggcg gctgcccgcg
     8641 cccatcccga cgtggccttc atcgtgtacc actcggggtt cgagaccggg atctccgaag
     8701 gccccttcac caggaggacg gcaggcgggg gcgtcaaccg gctgatcgca tccctcaagc
     8761 ggaacggcat ccggcccgga ggcaacgtct acgcggagct cgggtcgacc tggtggtacg
     8821 tgatgcgcta cccggaccag gccgcccacg tgctggggaa gctcctgaag cacctcgggg
     8881 aggacaacgt cctgtggggc acggattcga tctggtacgg ctcccctcag gatcagatcc
     8941 aggccttgcg atcgttccac atcagcgagg agtaccagga gcgcttcggc tatccggccc
     9001 tcaccaagag gatcaagcgg aagatcctgg ggctcaacgc cgccgacctc tacgggatcg
     9061 accccacctc ggttccctgc acgttcacga gacccgagct tcgcaggatc cggcgacagc
     9121 tgcggcgccc ggaccgcacc aacgggccca gcacttccgc cgaggccagg cgggtccggg
     9181 agcatcaccg cgggatgccc tgacaggccg tctcgccgtg ccggtcagtg cgagcggaag
     9241 gcctcctcga gccactccgc cacgacgtcc ggggcgatct tgacgtcgag caccaggggg
     9301 ccgtcgcggc gctcgagcca gccgtcgacc acgctcatgt cgtcaaggga ccgaacggtc
     9361 gcggcctcgg cccccagccc gcgcgccatc ccggccagat ccggatcggg gtagcgcacg
     9421 aggtcgacgg ggtagccctg cgggccgaag tggtggacct cggccccgta cgccgagtcg
     9481 ttgtagatga ccacgactaa cgggagcccg aggcgaacga gcgtctcgaa ctcccccgcg
     9541 gccatcatcg cgcccccgtc cccgaggcag gccacggtga gacgatcggg ccgggccacg
     9601 gccgccccga tcgccgacga cagccccaac ccgatcgatt ggaacgcctg cgtgaacacg
     9661 aagccggccg ggtccgggac cgagaggtac atcggcggcc agcccatgaa gtggccggag
     9721 tccaccgcca cgacgcgctc gggcgggagc gcctcctcca gggccttgga gaacgtccgg
     9781 gggtcgaggc ggccgtcctt cgaggcgtcc tcatggggct cgtcggccca ccgaccgccc
     9841 gcgatccgct ccgcgacgtc atcactgcgg cgccccggca cccgatggcc gcgccggtcg
     9901 agctccgcag ccaccgcctc ggccgtcagc cgagcgtcgc cgaccacgcc cagatcgatt
     9961 gggcggtgcg cccccagctc gtccgcgtcg cgatcgacct gggcgaccct cgccccgggc
    10021 ccgacgagct cgccgtggcg cgtggtccac atgttgaggg aggcgccgaa cgagacgacg
    10081 acatccgcct cccggatcag gcgaccggcc gtcggtgagg cgaagccgcc cgagatgccc
    10141 aggctccacg ggtttcccgc gaagagtccg tggccgttgg ccgaggtggc gagcagcccc
    10201 ccgacccggt tgccgagcgc ctcgatcgcg ggacccgccc cggccagcac cgcgccgcgc
    10261 ccgcccacga ggacggggga ggcggcggcg gccagcaggt cggcgatccg ctcgacgtcc
    10321 tcgtgggcgg gcgcggccgg ctccggcgcc tccggcgcct cgatctcatc gcccgccgcc
    10381 tcgccggcct ggacgtccag cggcatgctg aacaagaccg cgcgccggcc cacgaccgcc
    10441 gtccgccagg ccctcgccag atcctcgagc gccgtctcgg gcgaccgggc tcgctcggcc
    10501 accgccccca cggctgtggc caggccggcc tggtccacag cgaagttgga tcgtcctgcc
    10561 ccggccgacg tgtccgccgc caggaccatc agcggcgtcc tggccttggc cgcctccgta
    10621 agcgcggtca tcgcgttcgt gagccccgga ccctgatgta ccgtcaccac gcccgggcga
    10681 ccgctcaccc gggcgtatcc gtccgccatg ctcaccgccg cgctctcgtg ccgggccgcc
    10741 acgaagcgca cgcctcggtc caccatggcc tgcgtcacga ggaagttccc gctccccagg
    10801 agaccgaagg cgtcacggac ctccagcttc gccagggcca acccgatcgc ctccgctagg
    10861 cgcaccgctt cctcccccga ccgggcatct cgacctcggc actatggaat gggccgagac
    10921 gccgggtcaa ggccgcggct ccgctcggca ggcctctgcg gtcttggccg tgatggcggc
    10981 agggctcggg cagggcgacg ccagaagggg tgagcaggga acgagcgagg ggggcaaacg
    11041 gccgggtcat cgacccggcc gcttgccccc ctcagcgctc gccgtctgct caggaggcgc
    11101 gacggtagtc gtaccggtcg ccggccacgg cctcgagctt gtcgtgccag cgcgccccga
    11161 acatcccgcc gaggatcccg cccaggaaca tcaccacgag cgtggtgatc cccaggccga
    11221 tgccccaggt caggacgacg tcctcctcta cggggacgcg gaagtccgtg aacggcaccg
    11281 cgaagcgagc ctcaccgccg aacgcggcga cgaggccgac cgccagcccc atcaggagca
    11341 gggccagcac cgggacgagc aggccgttca ggagtcccgc cccgcgggcc atccttccgg
    11401 ccgtgtaccc gccccagaag tacgcgatga actgcgtcac gacgagggcg atccccacgc
    11461 cccatccgat ctcggtggcg ctggcgtcca cgccctcggc ggtcacgccg accgctaccg
    11521 cgatgcccgc gatcagcatc gtcaggagcg acatcgcccc gaacgccacg gcggtccccg
    11581 tgacgatcga gccgaagctc acccctccac gcacgtatcc ggggccatag gcagggcgct
    11641 cgtgcacgtg gtcgtcgtcg tgcacctcgc gccgctccac gcggtcgtcg accccgtcgt
    11701 tgtctcggtc cgaatcgagg acccggcgcc gctcgtcggc ccggatctcc tcctcggttc
    11761 tcgcatccac tcgctcgtgt gccatggtct tccacctccc gcatcgggct cccccatccc
    11821 ttacccgggt catcccccca acgaaccgcg cgttctcgcc cgctcccgag caggaagaag
    11881 gatctgatcc ggcacgacgt cgcccccgca agcgcatcga gcctctgctc gaccctctcg
    11941 caccgcacag ggagcataat ggggtcgatg cggacactgc gcaccccgat cccgttcgtc
    12001 cttctcgtcg cggtgctcgc cacgacagcg tgcgcctcgg accccgcccc cttccgggcc
    12061 gctgcaccga cggccgcacc gaccgcctcg tcgggagcta cgccggccgc tgccccccaa
    12121 cccggatccc cccggagcca gatctacgcg gcggtcatcc gccagctcgt gaccgtcgat
    12181 cacaccttcg gcggggcaaa gccccccttc gaccgcgtcc tcgttgtgga cggcgtcttc
    12241 cccgagccga agcgcgcgcc ctggggggcc gcgtccccgc gacatccctt ccccgagccc
    12301 ctcaagacgg cgatcgaggg ccatctcgag gatctcccgc cgctctgttt cattggcgag
    12361 gagcaggccc ctcgggacct cccgaccgcg caggcagccg tcatccggct gggcccgatc
    12421 gttcaggact gggagcatca ccgcgtccct gcctcgctgg aatgtggcag cgtctgcggc
    12481 atctggctca gctacatcct cgccgacgag gatgggcgct gggtcatcat cgaccgatcc
    12541 ggccccctcg cgatcgccta ggttccggcg accggttcgc cgatgggata tgactctccc
    12601 gtggagcggt tcgacgtcgc ggtgatcgga gggggcctcc tcgggctggc caccgcgcgt
    12661 catctcctag agcgccgtcc cgacctgcga ctggccgtcc tggagaagga atccgcgctc
    12721 gccctccacc agagcggccg gaactcgggc gtcgtccact cgggcatcta ctacaagccc
    12781 ggatcgctga aggctcggct gtgccgcgag gggcggtcga tgatcgagcg cttcgcggcc
    12841 gaccacgaca tcccctacga ggcctgcggc aagctcatcg tcgcggtcga ggaatcagag
    12901 ctcggtcggc tggctgagct ggcccgtcga gccggggcca acggcgtcga gatcgaggag
    12961 gtcggaccgg agcggatccg ggagctcgag ccgcacgccg tcggcatccg cggcctccac
    13021 tcgcccggca ccgccatcac ggacttccga cgggtcgccc tcgcgctcgg ggaggaggtg
    13081 ggacgcatgg gcgggacgat cctcctcgag cacggcgtca cgtcgatccg cggtcgaggg
    13141 gagcggcgac ggctcgagac gcgacgcgga cccgtcgggg cgcgcttcgt ggtcagctgc
    13201 gcggggctcc agtccgatcg ggtcgccgct cttacgggtg atcgcggcgg ccagcgcatc
    13261 gtgccgttcc gaggcgcgta ccagcggctc ggcgctcgcg ccgcgggcct ggtccagggg
    13321 ctcgtttacc cggtccccga tcctcgactc cccttcctgg gggtccactt cacgaggcgg
    13381 gtcgacggtg aggtctgggt ggggccgagc gccgtcctcg cgctggcccg cgagggctcg
    13441 aggccctggc aggtgaacct ccgcgacgtg gcctcgacgc tgcgcttcgg gggcttctgg
    13501 cggatggcgg cccgtcactg gcggacgggg ctggaggagg tgacgctcga tctcagcaag
    13561 cgtgccttcc tgaaggcgtg ccgccggtac ctgcccgagc tcgagcgcca ggacatcctc
    13621 cccggcccgt tcggcgtccg ggcccaggcg gtgaaccggg acgggtcgct ggaggacgac
    13681 ttctccctac ccgagagcca cggcgtcctc cacgtccgca acgccccgtc gcccggaggg
    13741 acggcctcgc tggcgatcgg caggacgctc ggcgaacggg tgctggctcg gctggacgag
    13801 cggtagctgc agggccggat caggtcgagt cgcgcaggag taccgcgatc gcgctgaggt
    13861 ccaactcggc gagcccggcc tccagcgccc gccggacgat ctcgcggttc gtctctccct
    13921 ggtccatgcg ggcgcccacc tgccgtgcca gatcgaagat gagatcgagg tccttcgcca
    13981 ccaggtcgag gctgaaggcg accggcgtct cgtccggctt caggaacgcc tcctgcttgt
    14041 agagcacgaa cggggcggcg accgcgctgt tggcgaagac gtcgtaggcg gcggggcgat
    14101 ccacccccgc cctctcggca agcacgagcg cctccgacag ggccacgtcg agggcgtgca
    14161 cgacggcatt gacggcgagc ttcatggtgg cccccgcccc gagctcgcct acgtgcatca
    14221 gcttccccga gagggcctcg aggacgggcc gggcccgggc gagcgcctcc tcctccccgc
    14281 ccaccatgat cgtcagcttg ccggtctcgg ccagcgagac gctgccagag acaggcgcat
    14341 ccaggaggct cgcgccgcgc tcctcgatcg gctctcggag cccgaggacc gtctcgcggg
    14401 cgatcgtgct catctcgcag acgacggtgc ccgggcggag ggtggcggcg accgcgcccg
    14461 agccgaggta gacgtcccgc accgcctcgt cgtcggccag gctgctcagg acgatctccg
    14521 cgctcccgac cgcctcgggc gcggtcgccg ccacctcgca cccgctggct tcggccaccg
    14581 cctcggcttt ctccggtgac cggttccaaa cgacgacgtc gaagccctcg cggcggaggg
    14641 tggcggccat ggcggcaccc atccggccca tgccgaggta ggcgaccctg ccgctcacgt
    14701 cagcgaccag cccccgtcca cggcgaggtt gacgccgttc accgcggggt tctccaacag
    14761 gaacaccgaa gcgtggacga tgtcctgcat ccggagcgga cgccccgtgg gggtcttctc
    14821 tcggaccgcc tcgagcacct ccggcggctt ggcggcccag aaggggctgt cggccacgat
    14881 cccggggtgg atcgcgttcg tgcggatagg cgccagctcc gacacgaacg tccggaccag
    14941 cccgaccacg cccgcgttga tcgtggtcac cgtggtcgag ccggggtagg gcttgtcttt
    15001 ggccagaccc ccgaacagga ggatcgagct ctcgtcgtgg aggcgcgagg tcagcgcgtg
    15061 caccacctcg gtgtagccca cgagcttcag cgtgaccagc cggatcgcgg cctgcacatc
    15121 gtagtcggcc acgcggttct cgtcccggta gatcgaggcg atcaccagat ggtcgacacc
    15181 ccccaccccc tccagcgcgg ccccgatctt ctccggctcg tgcaggtcga gcccgatcgc
    15241 ggtcgtgtcc ccgccgatct cctcggcgat cgccgagcag cgctcctcgt cgcgacccga
    15301 gatcacgact gggcggcccg ccgccgcgta gtgcgcggcc acctcccggc ccaggcccga
    15361 cgtcccgccg atcaccacca cgctgccgtc agccacgtcc cgcctccttc gagagcgcgt
    15421 ccttcaggta gtcccagtcc cgggcgaaac ggtaggagtg ccgcgcgggg ggctgaggcg
    15481 cctgggtctc gagccagcga acgatccccg ggcccgggtt cgagaacgaa tggacgcaac
    15541 ccaccccggc ccaggccacg tcgccggggc gcatccggta tcgctccccg tcgaacgtcg
    15601 cgtcgacctc gccctccacg atcaggtagg tctcctccag agggtggtcg tgaggcccgg
    15661 ccaccccgtc gggctcgtac tgcaccatga acatcgtgga gagctgagcg ccgagatccg
    15721 agtcgaccat catcttcacg gtgatcccgc tgtagacgag gagagccgtg cgcatgctgg
    15781 ccgagaccga gagcatgtcc tgggtctgct tgtcga
//
LOCUS       DASUTZ010000051         8034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_661766, whole genome shotgun sequence.
ACCESSION   DASUTZ010000051 DASUTZ010000000
VERSION     DASUTZ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8034
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661766"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..544)
                     /locus_tag="VF097_02450"
     CDS             complement(44..544)
                     /locus_tag="VF097_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261689.1"
                     /translation="MSWALEPAANPSILRFHVREELTKRTIETCPPATPPEMLDPVLA
                     LDDVRSVDLHRYRARINLSPGVEALGIMPSIERLLAERLGPAEYLPPDPPPQAYPVDH
                     EEPRIVAESLEMAGDQPILRALFETEGVAEAILGTGLLLVRLGRLFTWEESGDAVQRA
                     LDSAAG"
     gene            complement(541..1449)
                     /locus_tag="VF097_02455"
     CDS             complement(541..1449)
                     /locus_tag="VF097_02455"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEX6261690.1"
                     /translation="MDRRDRARGTMVGLVVGNALGIPFEGSAGASIPAGAVGEIPESE
                     ADAPWDDDVALAVMLAEAAIERGAMEVDDLAARLLAWYRENPRGIGILTSRVLVRLDR
                     GADPHAAAREVWEESGRTAAGNGAVMRCAPLALRWWRDPERLAAETLASAGITHADPR
                     CGWSAVTLNRALAAVLSDQAPDLEALAEDAWRGAGEEYGPEIAEAVVASAEAARPADL
                     SLDGGDMGYTLKTMQVGLWALVRAGDLEASLVSVIEAGGDTDTNGAVGGASLGCRDGL
                     EAIPARWLERAPRRERLLELADALIG"
     gene            1493..2815
                     /locus_tag="VF097_02460"
     CDS             1493..2815
                     /locus_tag="VF097_02460"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HEX6261691.1"
                     /translation="MLVRIRAAWEEFRSNLFAVPLIYLLLAVVVAQTMLEIDQRITQD
                     ELDVPLIFRSTVDSARALLSAVATATITVAGIAFSISLLLFQLASNQFSPRVLHGFFR
                     DTFTKQVLGLVVGTFTYCLLVLRAVRGAVETNGTPIIPNLSVALGLVAGIASILAIVA
                     FINHTAHSMHATEIIARITRETREQIDRLLESPEESDGRDPNPEDAPEAPEDAFVVTA
                     RSDGWVQQVDDGGLLDAAEDGGTVRLETFVGDFVPEGAALCSIWPPPADEERAVREAR
                     EAVRLGKSRTLQQDLGFGLRQLVDIALRALSTGINDPTTASEVVSHLGGLVHDVLVRD
                     LPPSRHADDRGRRVFRPREISHRGFVELAFAQIRVAAAGQPGVVRHLLDVLGPLAMMV
                     ERRGYPDRAEMVRQQARLALRGAEAAGILDVDMLDLLEQARRLDLSPA"
     gene            2859..3521
                     /locus_tag="VF097_02465"
     CDS             2859..3521
                     /locus_tag="VF097_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261692.1"
                     /translation="MTRRFGWGRVVALSSLVALGLTACGESDFDFVGSPDRKAYFKVP
                     LEWKEYSKRDLLAASGLDLSDVTQQTLPWLVAYDSAPDPSADHILELGAAPDHPVVLA
                     QARVLDFASRDQTSLGSIRNELYPVDELVQGDAAEIRDYEELVLDRGYRGIRIEFDVT
                     LGGNAEITAGNEVIRVHQIGVVDPGTETLYLFAIRCESHCFDENGKLIDQIVDSWTVK
                     EL"
     gene            3526..5994
                     /locus_tag="VF097_02470"
     CDS             3526..5994
                     /locus_tag="VF097_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6261693.1"
                     /translation="MSDDDKKGLEPMSHGQPALEYRSREREEERATRKPLDWWDRVKF
                     LALLGTAFLVMTWGTMARFAPQIGFVDAVRETWRDYWWMLALAGAELIRQLHFVTSEH
                     WAGYYRLWSQRIFGGFDRWTARWKDWTRFRISRVAKLLFFLAILSIVLGGILGRSPFT
                     VLFELPFLLFSWLPYVFQLAFGFFFIMVQFLGLFWFLSKGGVDVYYPDDVKTRFTDVW
                     GQDNVLDKIKENMVFLEEPEKIEEQGGYVPGGVLLWGPPGTGKTLMAEAVAGETGKPF
                     VFVEPGAFMNMFFGVGILKVKGLFRRLRKLALRYGGVIAFFDEADSLGNRGMVGQPGP
                     GGQPVGSTAFGSLCNGLSYLSPESAWQVLRAATEGGDAPEQPRRVVDRIMMGAGMGGG
                     GMGTLQALLTEMSGLKKPRGFFNRIVRRWLGMRPKPPPKYRILIMMATNRPDVLDPAL
                     LRPGRLDRIYKVGYPSKAGRIRTYQGYLEKVRHELTDEQVDKLATITPYYAGANIKDI
                     VNEALINAIRDGRETIQWKDVVKAKQLKDLGPPEDVEYIERERHAVAVHEACHAVAAL
                     RARQHLTIDIATIEKGGSYLGMVASIPPEDQFTRWRSEYEADIIVSLASLAGERLFFG
                     GDSSSGVSGDLETATQLMTLMEGYWGMGGTVASHGVTHRVGIGGGGRPGQGEQKKKDL
                     LQGGLGERIEERLHELYRRAEKLLRDNRAAVLAVAHALETRKTVTGDDIAAIVDGEEG
                     PIVDGRPYGTAAFRRAAETYHKAAVLAHKEHRAPDVTLPDLGELGNGQRRASGGGRGN
                     GRRSGNGRRAGTKRTGTRSSRKTK"
     gene            6021..6401
                     /locus_tag="VF097_02475"
     CDS             6021..6401
                     /locus_tag="VF097_02475"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEX6261694.1"
                     /translation="MVGRALLRRCPRCGRGGLFRRWFTMVEACPRCGLRFEREEGAFL
                     GSLSLNYGVTGLTLIALLAIWIVVSYPDLNARVATIGSLVVLAIVPALFYPFSKTLWA
                     AIDMLLHRMDPQDADTAWGGDPRS"
     gene            6405..7253
                     /locus_tag="VF097_02480"
     CDS             6405..7253
                     /locus_tag="VF097_02480"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6261695.1"
                     /translation="MAILLGLGAAAAWGVADFFGGFASRWVRATLAVVISQAIGLASL
                     LLLLPLFRHETMTSGAVLWGALAGMAGGFGLLNLYRGLGRGRMAVVAPVSAVIGAVVP
                     VAFDLAIGDPVGALTLAGVVIALAALALVSWHPGVEGRADGDSLLRSGLPEGLLAGLG
                     FGTFFVLFAQAGDASTYWPLLSLRVASVLMLSAIALATGSFARPGRRALGILVAAGVL
                     DVTANLLYLLGTQRGLLSVVAVLAALYPAATVLMARAILKERLHRTQVLGLAAAGLGI
                     VLITAG"
     gene            complement(7240..>8034)
                     /locus_tag="VF097_02485"
     CDS             complement(7240..>8034)
                     /locus_tag="VF097_02485"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018385731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX6261696.1"
                     /translation="GEPWPAPGKPFFFRAPATAVETLVDLGVDAVTLANNHALDFGEV
                     ALLDTMDLLDEGGIAHVGAGRDVDAARSPVLLTAKGFRLRVIGVTDHPEDFAASGRRA
                     GVALDALAGAPPPWLADAIAAPGADAVLVTPHWGPNMTPEPIPRVRAAAEALVDAGAT
                     VVAGHSAHVFHGVGGRVLFDLGDFLDDYAVDPDLRNDLGLLWLVTVDETGPGRIEAIP
                     LGLDFCFTRLADPEEAAWIGRRFREACSAFATQVAEEDGRLAVSPR"
BASE COUNT         1125 a         2866 c         2862 g         1181 t
ORIGIN      
        1 ccgctgagct aagcgcccga gactcccatg ctaccggcct cggtcacccg gcggccgagt
       61 ccagcgcccg ctgcacggcg tcgccggact cctcccaagt gaacaacctc ccgagccgga
      121 ccaggaggag gccggtgccg aggatcgcct cggccacgcc ctcggtctcg aagagcgctc
      181 ggaggatcgg ctggtccccc gccatctcca ggctctcggc cacgatccgc ggctcctcgt
      241 ggtccaccgg gtaggcctga ggcggcgggt ccggcggcag gtactccgcc gggcccagcc
      301 gctccgccag gagccgctcg atcgacggca tgatcccgag cgcctccaca cccggggaga
      361 ggttgatgcg ggctcggtag cggtggagat cgaccgacct cacgtcgtcg agcgccagca
      421 ccgggtccag catctcgggg ggcgtggccg gagggcaggt ctcgatcgtc ctcttcgtga
      481 gctcctcccg cacgtggaat cggaggatgg aggggttggc ggcaggctcg agcgcccagc
      541 tcatccgatc agcgcgtcgg ccagctcgag aaggcgctcg cgccgcggcg ctcgctccag
      601 ccaacgggcc gggatcgcct cgaggccgtc ccggcacccg agcgacgcgc cccccacggc
      661 gccgttcgta tccgtgtcgc cgcccgcctc gatcaccgag acgagcgagg cctcgaggtc
      721 gccggcgcgg accagcgccc acagcccgac ctgcatcgtc ttgagcgtgt agcccatgtc
      781 gccgccgtcg agcgagaggt cggccggccg agccgcctca gcggaggcca cgaccgcctc
      841 cgcgatctcg ggaccgtact cctcccccgc gccgcgccac gcgtcctccg cgagcgcctc
      901 cagatcgggc gcctggtccg acaggacggc ggccagcgcg cgattcagcg tcacggccga
      961 ccacccgcac cggggatcgg cgtgggtgat cccggcgctg gcgagcgtct cggccgccaa
     1021 tcgctcgggg tcccgccacc aacggagggc cagcggcgcg caccgcatca cggcgccgtt
     1081 cccggccgca gtccgtccgc tctcctccca gacctcgcgg gccgccgcgt gcgggtccgc
     1141 cccgcggtcg agccgaacca gaaccctcga ggtcaggatg ccgatcccac gcggattctc
     1201 ccgataccag gccaggaggc gggccgccag gtcgtccacc tccatcgccc cccgctcgat
     1261 cgcggcctcg gccagcatca ccgccagggc cacgtcgtcg tcccagggag catcggcctc
     1321 cgactcgggg atctccccca ccgctccggc cgggatcgaa gcccccgccg atccctcgaa
     1381 ggggatgccc agcgcgttgc ccacgacgag cccgaccatc gtccctcgag cccgatcccg
     1441 ccggtccatg ctcctcatcc ttccatgagc ggccgtggcc tatcatcggg gcgtgctggt
     1501 ccggatccgc gccgcgtggg aggagttccg cagcaacctc ttcgcggtgc ccctgatcta
     1561 cctcctgctc gcggtcgtcg tcgcccagac gatgctcgag atcgaccagc gcatcacgca
     1621 ggacgagctc gacgtccccc tgatcttccg ctcgacggtg gacagcgcgc gggcgctgct
     1681 gagcgccgtg gccacggcca cgatcactgt ggcgggcatc gcgttctcga tctcgctcct
     1741 cttgttccag ctcgcgtcga accagttctc gccgagggtc ctgcacggct tcttccgcga
     1801 cacgttcacg aagcaggtcc tcggtctcgt ggtggggacc ttcacgtact gtttgctcgt
     1861 tctgcgggcc gtgcgcggcg cggtggagac gaacggcacc ccgatcatcc ccaacctgtc
     1921 ggtcgcgctg ggcctggtgg cggggatcgc ttcgatcctg gccatcgtcg ccttcatcaa
     1981 ccacacggcc cactcgatgc acgccaccga gatcatcgcc cgcatcacca gggagacgcg
     2041 ggagcagatc gatcgcctgc tggagtctcc cgaggagagc gacgggcggg acccgaaccc
     2101 cgaggacgcg cccgaggcgc ccgaggacgc cttcgtcgtg acggcgcggt cggacggctg
     2161 ggtccagcag gtagacgacg ggggcctcct ggatgcggcc gaggatggcg ggaccgtccg
     2221 cctcgagacc ttcgtcggcg acttcgtccc ggagggtgcc gcgctctgct cgatctggcc
     2281 cccgccggcc gacgaggagc gcgccgtgcg cgaggcacgc gaggcggtcc ggctcgggaa
     2341 gtcgaggacg ctccagcagg atctcgggtt cggcctgcgc cagctcgtgg acatcgccct
     2401 tcgggccctc tccacaggca tcaacgaccc caccaccgcc agcgaggtgg tgagtcacct
     2461 ggggggactg gtccacgacg tcctcgtcag ggacctgccg ccgagccgac acgccgacga
     2521 ccgcggccgc cgcgtcttcc ggccccggga gatctcgcat cgaggattcg tcgagctcgc
     2581 cttcgcccag atccgcgtgg cggcggcggg ccagcctgga gtcgtgcgtc acctgctcga
     2641 cgtgctaggc ccgctggcga tgatggtgga gcggcgcgga taccccgatc gggcggagat
     2701 ggtgcgtcag caggcgcgcc tcgccctccg gggagccgag gccgccggga tcctcgacgt
     2761 ggacatgctg gacctcctgg agcaggcccg gcgcctcgac ctctcgcccg cttaggatcg
     2821 acccgacccg acgagccatc gagcggcata atgggcggat gacccgtcgg ttcggctggg
     2881 ggcgcgtcgt cgcgctctcc tctctcgtgg cgctcgggct caccgcatgc ggcgagtccg
     2941 acttcgactt cgtggggagc cccgaccgca aggcctactt caaggtgccg ctggagtgga
     3001 aggagtactc gaagcgggac ctgctggccg cctcgggcct ggacctcagc gacgtgaccc
     3061 agcagaccct gccgtggctg gtcgcgtacg actcggcgcc cgacccgtcc gccgaccaca
     3121 tcctcgagct gggcgccgcc cccgaccatc cggtcgtcct cgcgcaggcc cgcgtcctcg
     3181 acttcgccag ccgcgaccag acgagcctgg gctcgatccg caacgagctc tacccggtgg
     3241 acgagctggt ccagggcgac gccgccgaga tccgcgacta cgaggagctc gtgctcgacc
     3301 gaggctaccg ggggatccgg atcgagttcg acgtgacgct gggagggaac gccgagatca
     3361 ccgcgggcaa cgaggtgatc cgggtgcacc agatcggggt cgtggacccg ggaaccgaga
     3421 cgctctacct gttcgcgatc cgctgcgagt cccactgctt cgacgagaac ggcaagctga
     3481 tcgaccagat cgtggattcc tggaccgtga aggagctctg agacgatgag cgacgacgac
     3541 aagaagggtc tcgagccgat gagccacggc cagccggccc tcgagtaccg cagccgcgag
     3601 cgggaggagg agcgggccac ccgcaagcct ctcgactggt gggaccgggt gaagttcctg
     3661 gccctgctgg gcaccgcctt cctggtgatg acctggggga cgatggcccg gttcgcccca
     3721 cagatcgggt tcgtggacgc ggtgcgggag acgtggcgcg actactggtg gatgctcgcg
     3781 ctggccggcg ccgagctgat ccgccagctc cacttcgtca ccagcgagca ctgggcgggc
     3841 tactaccgcc tctggtccca gaggatcttc ggagggttcg atcgctggac cgcccggtgg
     3901 aaggactgga cccggttccg gatcagccgc gtggcgaagc tgctcttctt cctggcgatc
     3961 ctgagcatcg tgctcggcgg gatcctgggc cgctcgccgt tcacggtgct gttcgagctg
     4021 ccgttcctcc tgttctcgtg gctgccctac gtgttccagc tcgccttcgg cttcttcttc
     4081 atcatggtcc agttcctcgg gctgttctgg ttcctgtcga aggggggcgt ggacgtctac
     4141 taccccgacg acgtcaagac ccggttcacc gacgtctggg gccaggacaa cgtcctcgac
     4201 aagatcaagg agaacatggt cttcctcgag gagcccgaga agatcgagga gcaggggggg
     4261 tacgtgcccg gcggcgtcct gctgtggggc ccgcccggga ccgggaagac gctgatggcc
     4321 gaggccgtgg ccggcgagac cggcaagcct ttcgtcttcg tggagcccgg cgcgttcatg
     4381 aacatgttct tcggggtggg catcctcaag gtcaaggggc tgttccgcag gctacggaag
     4441 ctcgcgctgc gctacggcgg ggtcatcgcg ttcttcgacg aggcggactc gctggggaac
     4501 cggggcatgg tgggtcagcc gggccccggg ggccagcccg tgggctccac ggctttcggg
     4561 tcgctgtgca acgggctctc ctacctctcc ccggagtccg cgtggcaggt cctgcgagcc
     4621 gcgaccgagg gtggcgacgc gcccgagcag cctcggcgcg tggtggatcg catcatgatg
     4681 ggagcgggga tgggcggcgg cggcatgggc accctccagg ccctcctcac cgagatgtcc
     4741 gggctgaaga agccccgcgg gtttttcaac cggatcgtca ggcgatggct cgggatgcgt
     4801 cccaagccgc cccccaagta ccggatcctc atcatgatgg ccacgaaccg gccggatgtg
     4861 ctggaccccg ccctgctgcg tccgggccgc ctggaccgca tctacaaggt cggctacccg
     4921 tcgaaggcgg gccggatccg cacctaccag ggctacctgg agaaggtgcg gcacgagctc
     4981 accgacgagc aggtcgacaa gctcgccacg atcacgccgt actacgccgg cgcgaacatc
     5041 aaggacatcg tcaacgaggc gctgatcaac gcgatccggg acgggcgaga gacgatccag
     5101 tggaaggacg tcgtcaaggc caagcagctg aaggacctcg gaccgcccga ggacgtcgag
     5161 tacatcgagc gcgagcgcca cgcggtcgcg gtgcacgagg cgtgccacgc ggtggccgcc
     5221 ctccgcgcgc ggcaacacct gacgatcgac atcgccacga tcgagaaggg cggctcgtac
     5281 ctcggcatgg tcgcctcgat cccgcccgag gaccagttca ccaggtggcg gtccgagtac
     5341 gaggccgaca tcatcgtcag cctcgcctcg ctggccgggg agcggctgtt cttcggcggc
     5401 gactcctcct cgggggtctc gggcgacctg gagacggcca cccagctcat gaccctgatg
     5461 gagggctact ggggcatggg cggcaccgtg gcctcccacg gcgtcaccca ccgcgtgggg
     5521 atcggcggcg gcggacggcc ggggcagggc gagcagaaga agaaggacct gctgcaggga
     5581 gggctcggcg agcggatcga ggagcgcctc cacgagctct accggcgtgc cgagaagctg
     5641 ctccgcgaca accgcgccgc cgtgctcgcc gtggcgcacg ctctcgagac gcggaagacc
     5701 gtgaccggtg acgacatcgc cgcgatcgtg gacggagagg aggggccgat cgtcgacggg
     5761 cggccgtacg ggaccgcggc gttccggcgg gcggccgaga cctaccacaa ggcggcggtg
     5821 ctggcacaca aggagcaccg cgcccccgac gtaaccctcc ccgacctcgg cgagctgggg
     5881 aacgggcagc gccgggcctc gggaggcggt cgcgggaacg gacgacgatc ggggaacggc
     5941 aggcgggccg gcaccaagcg gacggggacc cggtcctcac ggaagacgaa gtgagggtcg
     6001 ctcgtcccgg gatcggccgg atggtcggcc gcgccctcct ccggcgatgc ccccgctgcg
     6061 gccggggcgg gctcttccgc cgctggttca cgatggtcga ggcctgcccc cgctgcggcc
     6121 tgcgcttcga gcgcgaggag ggagccttcc tcgggtcgct ctcgctcaac tacggagtca
     6181 ccgggctgac gctgatcgcg ctcctagcga tctggatcgt cgtctcgtat cccgacctca
     6241 acgcgcgggt ggcgacgatc ggctcgctcg tcgtgctggc gatcgtcccc gccctcttct
     6301 atccgttctc caagacgctc tgggcggcga tcgacatgct gctgcatcgg atggacccgc
     6361 aggacgccga cacggcgtgg ggcggggatc ccaggagctg acgggtggcc atcctgctgg
     6421 ggctcggggc ggcggcggcc tggggcgtgg ccgacttctt cggcggcttc gcctcccgct
     6481 gggtgcgggc cacgctggcc gtcgtcatct cgcaggcgat cggcctggcc tcgctcctcc
     6541 tcctgctccc gctgttccgc cacgagacca tgacgagcgg ggcggtgctg tggggcgcgc
     6601 tcgcggggat ggccggcggg ttcgggctgc tgaacctgta ccggggcctg ggacggggcc
     6661 ggatggccgt ggtggcgccc gtgtcggccg tgatcggcgc ggtcgtgccg gtcgctttcg
     6721 atctggcgat cggggatccg gtgggggcgc tgacgctggc cggggtcgtg atcgcgctgg
     6781 cggcgctggc gctcgtgtcc tggcaccccg gagtcgaggg gcgagcggac ggagactccc
     6841 tgctccggag cgggctgccc gagggccttc tggcgggcct cgggttcggg acgttcttcg
     6901 tgctcttcgc ccaggccggc gacgcctcca cctactggcc cctgctctcc ctgcgcgtgg
     6961 cctcggtgct gatgctcagc gcgatcgcgc tggccacggg cagcttcgcg aggccgggtc
     7021 ggcgagcgct cgggatcctc gtcgccgctg gcgtgctcga cgtgacggcg aacctcctct
     7081 acctgctcgg gacacagcgg ggcctgctct ccgtggtggc cgtgctcgcg gcactgtacc
     7141 cggcggccac ggtcctcatg gcgcgagcga tcctgaagga gcgcctgcac cggacgcagg
     7201 tccttgggct ggccgcggcg ggactaggga tcgtgctgat caccgcgggc tgacggccag
     7261 ccggccgtcc tcctcggcca cctgggtggc gaaggccgag caggcctcgc ggaagcgcct
     7321 cccgatccat gcggcctcct cggggtcggc gaggcgcgtg aagcagaagt cgaggccgag
     7381 ggggatcgcc tcgatccgcc cgggtccggt ctcgtccacg gtcacgagcc acaggagccc
     7441 gagatcgttg cgcaggtcgg gatccacggc gtagtcgtcg aggaagtctc cgaggtcgaa
     7501 caggaccctg cccccgacgc cgtggaagac gtgggccgaa tggccggcca caacggtggc
     7561 gcccgcatcg acgagggcct ccgcggcggc ccgtacccgg gggatcggct cgggggtcat
     7621 gttgggtccc cagtggggcg tgaccagcac cgcgtccgcg ccgggggcgg cgatcgcgtc
     7681 ggccagccag gggggcggcg cgccggcgag cgcgtccagc gcgacgccgg cgcgacgccc
     7741 cgacgccgcg aagtcctcgg ggtggtccgt cacgccgatc acccgcagcc ggaagccctt
     7801 cgccgtcagc aggaccgggg accgggcggc gtcgacgtcg cgccccgctc ccacgtgcgc
     7861 gatcccccct tcgtccagga gatccatcgt gtcgagcagg gccacctcgc cgaagtcgag
     7921 cgcatggttg ttggccagcg tcacggcgtc cacgcccagg tccaccaggg tctcgaccgc
     7981 cgtggccggc gcccggaaga agaacggctt cccgggcgcc ggccacggct cccc
//
LOCUS       DASUTZ010000052         6504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1827545, whole genome shotgun sequence.
ACCESSION   DASUTZ010000052 DASUTZ010000000
VERSION     DASUTZ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6504
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1827545"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..452
                     /locus_tag="VF097_02490"
     CDS             48..452
                     /locus_tag="VF097_02490"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX6261697.1"
                     /translation="MAALQELLDSAPEDRRPILRAVHKAIRAAAPKLKMKVWRQKFWG
                     GTDQTILGYGDITYQSSAGEDVEWFKIGLANQKGYVSVYVSVTKDRRYLPEIYGSRLG
                     KVKVGRSSVSFRKLEDLDLEVLREMVAEAATA"
     gene            complement(583..777)
                     /locus_tag="VF097_02495"
     CDS             complement(583..777)
                     /locus_tag="VF097_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX6261698.1"
                     /translation="MELTSQEGINHTFVVEEAGDEVVAAADPGQTDTGTIELDAGSYT
                     FYCDVPGHREARMEGTLEVR"
     gene            complement(768..956)
                     /locus_tag="VF097_02500"
     CDS             complement(768..956)
                     /locus_tag="VF097_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261699.1"
                     /translation="MRARMLIMLVAGALVLGACGGDDGGDDGAGGGGSADVSVTGTDA
                     LAFEPSELSAERARSPWN"
     gene            1289..1756
                     /locus_tag="VF097_02505"
     CDS             1289..1756
                     /locus_tag="VF097_02505"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1418..1420,aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX6261700.1"
                     /translation="MLEEGARAPTFTLPDQDGNPVSLSDYPGSWVILWWFVVADTPGU
                     TMEGQGFRDRAPEFDELGAEILGVSYDPPEAQRAFAQKQGFAFRLLSDGDRTVAAAYG
                     TRRPDDHERAAFPRRFTYLIDPEGKVARSYVVKDVAAHPGEVLEDLRGLARGA"
     gene            1753..2541
                     /locus_tag="VF097_02510"
     CDS             1753..2541
                     /locus_tag="VF097_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015577189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01458 family HAD-type hydrolase"
                     /protein_id="HEX6261701.1"
                     /translation="MSRGAAGVLLDVDGVLVTSWEPLPGAVETIAWLRGANVPFLLVT
                     NTTTRSRASLARDLAPFEVGPDDLLTAPMATAAYLRTEHPGARVHLLAAGEVAEDLEG
                     IELVDSRAEVVVIGGASEPDPEHPDATFTYENLNRAFGMLLDGARLVAMHRNLSWRTS
                     RGIVLDSGAFVVGLERAAKVEAEVVGKPSAAFFRAALERLGMPADRALMVGDDIEADV
                     EGARSAGIRGVLVRTGKFRPEHLEVARPDHVIDSIADLPGLLGG"
     gene            2634..2804
                     /locus_tag="VF097_02515"
     CDS             2634..2804
                     /locus_tag="VF097_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261702.1"
                     /translation="MNEDPLKRRLRDRDFARAYRSWVRLLVDRARHRGSTRRVVRLPK
                     GESLRPPKSLAG"
     gene            complement(2806..3924)
                     /locus_tag="VF097_02520"
     CDS             complement(2806..3924)
                     /locus_tag="VF097_02520"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX6261703.1"
                     /translation="MFGNSWRVGRVRGIELRIDISWAIIAVLISYSLYWRYVEIYRGI
                     DNAYAILLGVGSAILFFGSVVFHEFAHAFTAQSRGIPVKSITLFLFGGATHAKVEAKG
                     PGAEFLIALVGPLSSLVLAGAFALLGTLGDAFLPREVAGAFGYLAFVNLALAVFNLVP
                     GFPLDGGRVLRAIIWGATGSLSRATRIASIAGQGFGYLLVAAGLFFVFNGALGAGIWF
                     GAIGWFLTQAARMSYEQLQLRRMLEDVDAEDVMASTVSSIPADITLRQAVDDHFMRSD
                     HSAFPVVDGDEAVGLLSLRRVKQVPRQEWGSRTVRETMGKLDEGCTVSPMTSMADVVE
                     RLEGENRRVVVVEDGEVVGIISPSDVARWLQRRQLVES"
     gene            3999..4775
                     /locus_tag="VF097_02525"
     CDS             3999..4775
                     /locus_tag="VF097_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014057000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX6261704.1"
                     /translation="MPELPDVEGYRRYLARHAQGRRVERVRADPTILRNTTPQALGRA
                     LVGGVLGPPRRHGKWLSCPVDGPTLVLHFGMTGSLVWSGEEPDRHPYDRVILEVEGGE
                     LRYRDMRKLGGVWVVPEGVDAESVTGHLGPDAQRLERDDLVELLARRRGGVKAALMDQ
                     RMVAGLGNLLVDEILWRARVHPRRAIGDLGRQDLERIADRTEEVLRLSIELGGVPPDE
                     TWLMGIRDGRDATCPRCGTPLERVKAGGRTSVFCPRCQRP"
     gene            4772..5665
                     /gene="ligD"
                     /locus_tag="VF097_02530"
     CDS             4772..5665
                     /gene="ligD"
                     /locus_tag="VF097_02530"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020039395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX6261705.1"
                     /translation="MKLTIGERTIDVGNEGKVFFPESGITKGDVVRYYARVAEAMLPH
                     VRGRPVSMERLPDGLGGTRWYQKEAPDHFPDWIERAEVPKKDGVVHHVVLREAADLVY
                     LANQGCLTPHVLLSTAEHLDRPDRMIFDLDPSVEDVGVLRDGARALRDLLEALELAPF
                     VMTSGSRGFHVTVPLAAEDDTEAVHRFAYDVSRLIVRDDPDRFTIEHRKAKRGDRVLV
                     DYFRNGWAQTAVPPYAVRARPGAPIAAPLDWEELGRTHPQRYTVRNAFRRLGRKPDPW
                     REIERHARHLGGPRKLLDAMR"
     gene            complement(5668..6249)
                     /locus_tag="VF097_02535"
     CDS             complement(5668..6249)
                     /locus_tag="VF097_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEX6261706.1"
                     /translation="MPEGARGLVVFAHGSGSSRHSPRNQQVAGALQPRALGTLLFDLL
                     TPEEDEVDRGTASLRFDIPLLAERVEGAVAWVREEVPGLPVGLFGASTGAAAALVAAE
                     HVPDDVRAVVSRGGRPDLAGDSLPQVVVPTLLIVGSLDTEVLGMNRDAMARMERADVA
                     LEVVEGASHLFEEPGKLELVGELARDWFAAHLS"
     gene            complement(6314..>6504)
                     /locus_tag="VF097_02540"
     CDS             complement(6314..>6504)
                     /locus_tag="VF097_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020519067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6261707.1"
                     /translation="VTLSPSDAVAQAVAEMRDKAIRRLPVVDEEGRPVGIVSIGDLAV
                     ERDADSALADISAAPPSD"
BASE COUNT          952 a         2309 c         2365 g          878 t
ORIGIN      
        1 cgtggcgtcg cgcccccgcc cccgccccct cgggtacgat ccggttcatg gctgctctgc
       61 aggagctcct cgactccgcc cccgaggacc gccgccccat cctccgcgcc gtccacaaag
      121 cgatccgcgc cgccgccccc aagctgaaga tgaaggtctg gcggcagaag ttctggggcg
      181 gcaccgacca gacgatcctc gggtacggcg acatcaccta ccagagctcc gcgggggagg
      241 acgtcgagtg gttcaagatc ggcctggcca accagaaggg ctatgtcagc gtctacgtga
      301 gcgtcaccaa ggaccgcagg tacctccccg agatctacgg ctcccgcctg ggcaaggtga
      361 aggtcgggag gtcctcggtc tcgttccgga agctggagga cctcgacctg gaggtcctca
      421 gggagatggt ggccgaggcg gccacggcct gagggtgcgc cctaccgacc gcgacgggtg
      481 gcggcgatgg ccgcggccgc cggcagcgag aagaacgcac ccgtcgctcc ctccctcgat
      541 cgaccgtcga agcctggcat gggaggaagc gacggtgctt cgtcaccgga cctcgagggt
      601 cccctccatc ctggcctcgc ggtggcccgg gacgtcgcag tagaacgtgt agctgccagc
      661 gtcgagctcg atcgtgcccg tgtcggtctg gccgggatcc gcggcggcca cgacctcgtc
      721 gccggcctcc tcgaccacga acgtgtggtt gatgccctcc tgggacgtca gttccacggt
      781 gatcgagccc gctcggcgct cagctcgctc ggctcgaacg ccagcgcgtc ggtccccgtc
      841 accgagacgt cggcgcttcc gccgccgccg gccccgtcgt cgcctccgtc atctcctccg
      901 caggcgccca gcaccagcgc gcccgcgacc agcatgatca gcatgcgtgc tcgcatcgct
      961 ctccttccgg ttgtcggatg ggccgtcctg ggttcgcggg cctccggacg accccctcga
     1021 tgctaacggc cctccctcgc tatcgtctgc gttccatcct tcaggcgccc tccgaacctg
     1081 ccgaaggggg aggtatgagc tggcgcagcg actcggcggg cctcgcgctc cttctgaccc
     1141 gaccgacggg cgaggaagac cgcgaacggg cccgcgctcg cgacggccgc cgccgcggcg
     1201 ttcgccgagg cctgcgacgc cgacgaccgc tccagacccg cgatccccgg cactgacccc
     1261 gccgcggcgt ccgagtagga tgccggacat gctcgaagag ggcgcacggg cgccgacgtt
     1321 cacgcttccc gaccaggacg gaaatcccgt gtccctgagc gactacccgg ggagttgggt
     1381 gatcctctgg tggttcgtcg tcgccgacac ccctgggtga acgatggagg gccaggggtt
     1441 ccgtgatcga gcccccgagt tcgatgagct gggcgccgag atcctgggcg tctcctacga
     1501 ccctcccgag gcgcaaagag cgttcgccca gaagcagggg ttcgccttca ggctgctctc
     1561 ggacggggac cggaccgtcg ccgcggccta cgggacccgg cgccccgacg accacgagcg
     1621 ggcggcgttc cccaggcggt tcacctacct catcgacccc gaggggaagg tggcccggag
     1681 ctacgtggtg aaggacgtgg cggcgcatcc cggggaggtc ctggaggatc tgcggggcct
     1741 ggcccgcggg gcatgagccg gggcgccgcg ggcgtgctgc tcgacgtgga cggtgtcctc
     1801 gtgacgtcat gggagccgct cccgggggcg gtggagacga tcgcgtggct gcgcggtgcg
     1861 aacgtcccct tcctgctcgt cacgaacacg acgacccggt cgcgcgcctc gctggcccgg
     1921 gacctcgccc cgttcgaggt gggtccggac gacctcctga ccgcgccgat ggccacggcg
     1981 gcgtaccttc gcaccgagca ccccggggcc agggtccacc tgctggctgc cggcgaagtg
     2041 gccgaggacc tcgagggcat cgagctggtg gacagccggg ccgaggtcgt ggtgatcggg
     2101 ggtgcgagcg agcccgaccc ggagcacccg gacgcgacgt tcacatacga gaacctgaac
     2161 cgggcgttcg gcatgctcct cgacggggcc cggctcgtgg cgatgcaccg caacctctcg
     2221 tggaggacga gccgcggtat cgtcctcgac tcgggggcgt tcgtcgtggg gctggagcgg
     2281 gcggcgaagg tcgaggccga ggtcgtgggg aagccgtcgg cggccttctt ccgggcggcg
     2341 ctggagcggc tggggatgcc cgccgaccgc gcgctcatgg tgggcgacga catcgaggcc
     2401 gacgtcgagg gcgcgcggtc ggcgggcatc cggggggtcc tcgtccgcac cggcaagttc
     2461 cggcccgagc acctcgaggt cgcccgcccc gaccacgtga tcgactcgat cgccgatctc
     2521 ccgggcctcc tcggagggtg aggtccgtcg cgaatgggcc gaacggccga taccctctct
     2581 cgccatctgt cgtaaggttg ggattcgaga cgaagggtag ggcccggatg cccatgaacg
     2641 aggacccgct caagaggcgc ctccgtgatc gggacttcgc ccgcgcctac cgctcctggg
     2701 tgaggctcct ggtggatcgt gcgaggcacc gcggatcgac gcgccgcgtg gtccggctgc
     2761 cgaagggcga gtcgctgagg ccgcccaagt cgctcgccgg ctgactcagg actcgaccag
     2821 ctggcggcgc tggagccagc gtgccacgtc cgacggcgag atgatgccca cgacctcgcc
     2881 gtcctcgacc acgacgaccc ggcggttctc gccctccagc cgctccacga cgtcggccat
     2941 cgaggtcatg ggcgagaccg tgcagccctc gtcgagcttg cccatcgtct cccgcaccgt
     3001 cctcgagccc cactcctgac gcggcacctg cttcacgcgt cgcaggctga gcagacccac
     3061 ggcctcgtct ccgtccacga ccgggaacgc gctgtggtcc gagcgcatga agtggtcgtc
     3121 cacggcctgg cgcagggtga tgtccgccgg gatcgagctg acggtggagg ccatcacgtc
     3181 ctcggcgtcc acgtcctcga gcatccgccg gagctggagc tgctcgtagc tcatccgggc
     3241 ggcctgcgtc aggaaccatc cgatcgcccc gaaccagatg cccgcgccga gcgccccgtt
     3301 gaaaacgaag aagaggccgg cggccacgag gaggtacccg aagccctggc cggcgatgct
     3361 ggcgatccgg gtcgccctcg acaggctgcc cgtggcgccc cagatgatcg cgcgcagcac
     3421 ccgcccgccg tcgagcggga acccggggac gaggttgaag acggccaggg ccaggttgac
     3481 gaaggcgagg tagccgaacg cgccggccac ctcccggggg aggaaggcgt cgcccagggt
     3541 ccccaggagg gcgaaggccc ccgccaggac caggctcgac agcgggccca ccagggcgat
     3601 caggaactcc gcaccgggtc ctttcgcctc caccttggcg tgcgtggcgc ccccgaagag
     3661 gaagagcgtg atgctcttga cggggatgcc tcgcgactgc gcggtgaacg cgtgggcgaa
     3721 ctcgtggaag acgaccgagc cgaagaagag gatggcggag ccgacgccga gcaggatggc
     3781 gtaggcgttg tcgatgcccc ggtagatctc gacgtaccgc cagtacagcg agtaggagat
     3841 gagaacggcg atgatggccc acgagatgtc gatgcggagc tcgatgccgc ggacgcggcc
     3901 cacgcgccag gagttgccga acatgggccc agcctacccg agtccatccg aagggaaccc
     3961 ggagcgtagg ctcgcggggt cgcgggaagg tcaccgggat gcctgagctg cccgacgtcg
     4021 aggggtaccg ccgctacttg gcccgccacg cgcaggggag acgtgtggag cgcgtccgcg
     4081 cggatcccac gatcctcagg aacaccactc cccaggccct gggcagagcg ctcgtgggcg
     4141 gcgtgctggg cccgccccgg cgccacggga agtggctctc gtgccccgtc gacggcccga
     4201 cgctcgtcct gcacttcggc atgacgggga gcctcgtctg gtcgggggag gagcccgacc
     4261 gccaccccta cgaccgcgtg atcctggagg tcgagggcgg cgagctgcgc taccgcgaca
     4321 tgcggaagct ggggggcgtc tgggtggttc ccgagggcgt ggacgccgag tcggtcacgg
     4381 gccacctggg ccccgacgcc cagcgcctcg agcgggacga cctcgtggag ctcttggcgc
     4441 gcaggcgagg gggcgtgaag gcggcgctga tggaccagcg gatggtggcc gggctaggga
     4501 acctcctggt ggacgagatc ctgtggcggg cccgggtcca ccccaggcgg gcgatcgggg
     4561 acctgggccg gcaggacctg gagcgcatcg ccgatcgcac cgaggaggtc ctccgcctct
     4621 cgatcgagct cggtggggtg ccgccggacg agacgtggct catggggatc cgcgacgggc
     4681 gcgacgcgac ctgcccccgc tgcggcacgc cgctggagcg ggtcaaggcc ggggggagga
     4741 cgtcggtctt ctgcccgagg tgccagcggc catgaagctg acgatcggcg agcgaacgat
     4801 cgacgtgggg aacgagggca aagtcttctt cccggagagc ggcatcacga agggggacgt
     4861 cgtccggtac tacgcgcggg tcgccgaggc gatgctgccc cacgtgcggg gccggccggt
     4921 ctcgatggag cggctccccg acggcctggg cgggacccgc tggtaccaga aggaggcccc
     4981 cgaccacttc ccggactgga tcgagcgggc ggaggtcccc aagaaggacg gcgtcgtgca
     5041 ccacgtcgtg ctgcgggagg cggccgacct cgtctacctg gcgaaccagg ggtgtctcac
     5101 cccgcacgtc ctgctctcga ccgccgagca cctcgaccgg cccgatcgga tgatcttcga
     5161 cctggacccc tcggtcgagg acgtgggcgt gctgcgggac ggggcccgag ccctgcggga
     5221 cctgctggag gcgctcgagc tggctccctt cgtgatgacg agcggctcgc gaggcttcca
     5281 cgtgacggtg ccgctggcgg ccgaggacga cacggaggcg gtccaccgct tcgcctacga
     5341 cgtgtccagg ctgatcgtcc gggacgaccc cgaccggttc acgatcgagc accgcaaggc
     5401 gaagcgggga gaccgcgtgc tcgtggacta cttccggaac ggctgggcgc agacggcggt
     5461 cccgccctac gccgtgcggg cccggcccgg cgcgccgatc gcggcgcccc tggactggga
     5521 ggagctgggc cggacgcatc cccagcggta cacggtccgg aacgccttcc ggcggctggg
     5581 ccggaagccc gatccgtgga gggagatcga gcggcacgcg cggcacttgg gggggccgag
     5641 gaagctcctc gacgcgatgc gctgacgtca ggacaggtgg gccgcgaacc agtcgcgggc
     5701 cagctcgccg accagctcca gcttccccgg ctcctcgaac aggtgggagg cgccctccac
     5761 cacctccaga gccacgtcgg cccgctccat ccgggccatc gcgtcgcggt tcatgccgag
     5821 cacttcggtg tcgagggatc ccacgatcag cagggtggga accacgacct gggggagcga
     5881 gtcgccggcg aggtcggggc ggccgccgcg cgacaccacc gcgcgcacgt catccgggac
     5941 gtgctcggcc gcgacaagtg cggcggccgc cccggtgctg gccccgaaca gccccacggg
     6001 gaggcccggg acctcctccc ggacccacgc cacggcgccc tccacccgct cggccagcag
     6061 ggggatgtcg aagcgcaggg acgccgtccc tcggtcgacc tcgtcctcct cgggcgtcag
     6121 gaggtcgaac aggagcgtcc ccagggcccg cggttggagg gccccggcca cctgctggtt
     6181 cctcgggctg tggcggctgg acccgctccc gtgggcgaag acgacgaggc cgcgggcgcc
     6241 ctccggcacg tcgaggaacc ccccgacgcg gaccggaccc gcctcgacct cgatctcgtc
     6301 cgttcctgcc tcctcagtcg ctcggcggag ccgcgctgat gtcggccagg gccgagtcgg
     6361 cgtcgcgctc cacggcgagg tccccgatcg agacgatccc cacgggccgg ccttcctcgt
     6421 ccacgacggg gagccggcgg atcgccttgt cccgcatctc ggccacggcc tgggccacgg
     6481 cgtcggatgg cgagagcgtc acga
//
LOCUS       DASUTZ010000053        14294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1050235, whole genome shotgun sequence.
ACCESSION   DASUTZ010000053 DASUTZ010000000
VERSION     DASUTZ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14294
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050235"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VF097_02545"
     CDS             <1..508
                     /locus_tag="VF097_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261708.1"
                     /translation="AAAAEVEALEAEEDPAAREPIARRLEDEVAKRAREVASYLEPSL
                     TTPFAVACVPDAAYAACRKAHAVAFRHGVVIVAYSAALPVLLSLYALGSRYGQSGDVE
                     ACLAELESVLEGMEQTLENRVARATTMLQNAADEWRAGLGRARWAVAKGRGTAGALED
                     ERRLEAVE"
     gene            531..860
                     /locus_tag="VF097_02550"
     CDS             531..860
                     /locus_tag="VF097_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261709.1"
                     /translation="MSFEDALRDLGFVFSRRGRGGETWEHRATPYLTHYVQLLEDGTA
                     LFTWEVAIGELMAAHGLQVGSDEHLNTFLFPREDARGPRDAAWVSSQMDAVEELLAAI
                     SLLAAQP"
     gene            888..1592
                     /locus_tag="VF097_02555"
     CDS             888..1592
                     /locus_tag="VF097_02555"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEX6261710.1"
                     /translation="MEIERALVAFAHPDDAEFLCGGTVALWAKQGVEVTYVCATDGSA
                     GWNAPGMTREEIAARREEEMREVGDLLGVTEVRFLGYPDGMLEPSLDLRRSLTREVRR
                     ARPDVLVSFDPSELWSGRSYISHPDHRAVGEAVLAVTACDAPTRPQFPELLEEGLEPF
                     KVRRVWLASDEGEHIVDIGEAIDLKVQAVQAHQSQMENMGEFDADARVRKWARDQAEG
                     TPHEFVERFRAFRIEV"
     gene            1595..2101
                     /locus_tag="VF097_02560"
     CDS             1595..2101
                     /locus_tag="VF097_02560"
                     /inference="COORDINATES: protein motif:HMM:NF036411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5319 family protein"
                     /protein_id="HEX6261711.1"
                     /translation="MADEEARPLDPDERRNIEADLEDLSAMRSVFEGQGMRGVAIACP
                     ECQTDHFYGWDLIIESLEHMLETGEPRMHEPAFDPREEDYVLWDYGKGYVDALADAGL
                     KETPGSEVTLLACAWCDTPVEAHFSYCPKCGRSMAPARLLRELVSRGMDEREARALLV
                     KAGFDPFR"
     gene            complement(2073..2645)
                     /locus_tag="VF097_02565"
     CDS             complement(2073..2645)
                     /locus_tag="VF097_02565"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6261712.1"
                     /translation="MRGGVPPRDEGRQAARPLLQGPPPRQGAAGLHPRGGPLSLNRYR
                     FRSVWRIPAPPEVVLDLLHELDAWTRWWPEIRESRRVDEDRAVVRVRSALPYDLHLLL
                     TRRRDTETVLEAGIEGDLRGFARFRLRPDGDGSTRLLFEQEVEARKPLMRRLAPIARP
                     LFRWNHARMMRSGRRGLAALVATGTGRSRP"
     gene            complement(2531..5026)
                     /gene="ligD"
                     /locus_tag="VF097_02570"
     CDS             complement(2531..5026)
                     /gene="ligD"
                     /locus_tag="VF097_02570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX6261713.1"
                     /translation="MARGEPFTVETPRRLEAEREGDHWWVEAAGRRVRLSNLPKAYWP
                     DDGYTKGDLLAYYWNVADTVLPYLRGRPLTMRRMPDGIEGGEFYEKNAPSHTPEWMPR
                     CPVLDEEGSKTIEYLRVEDEAGLLFVANLGAIEFHPLHSRCESVDRPDYLFFDLDPFE
                     PITFGDVRAVARHVRAALEALGLTSYPKTSGATGMQIYVPIELGPSYQQVRDFVGAVG
                     RLILKADPDRVTMEWEVSKRAGKVFIDHNMNRQGANIAAAYSVRPQPGATVSAPVTWD
                     EVEEGIEPGDFTIVTMHKRLAEVGDLFRGVLEEPVDLRPALEALGMDPRVEEAEATGA
                     PTAGEGEPASEELTADPKTDDGDPAARLAKYGEMRDFAETPEPPPVPAGGDGRSFVIQ
                     KHEATRLHYDLRLEEGGVLVSWAVPRGLPLKPGDRRLAVHTEDHPLDYGEFQGWIPEG
                     HYGAGEVRIFDRGTYEPLDWTDDKVSFRLHGTRHRGEYHLVKTDRDWLIFLGKGSDAP
                     QPEPPPAFEPMLADPGHDPFDDPGWRFEPKLDGVRTLAYVTTDATRLLSRKGRDQTAQ
                     YPELDNLARWTNALWAVLDGEIVAVDERGRPSFERLQERINLSAPSDIEKARRRIPVR
                     LYAFDLLWLDERDLTNEPLEERRRLLEGIVTESDRLGLTLYLDGEGERLFEAAKGLGY
                     EGIVAKKLGSRYQPGRRSKDWGKVKAVKRQDCVILGWTRGEGSRASSFGALLLGAYVG
                     DELRWVGQVGTGFTEQLTLQVLDRLREIREEEPPIGDRELASVKGATWVRPELVCEVE
                     YLHVTKAGKLRAPSFKGLRPDKAPQDCILEEAP"
     gene            5108..5929
                     /locus_tag="VF097_02575"
     CDS             5108..5929
                     /locus_tag="VF097_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018350239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX6261714.1"
                     /translation="MPRTMWKGAISFGLVSIPVRVFPATEEKTLRFNQLHDEDHGRIK
                     YQRVCAKCGKEVPYENIVKGYEYEKDRYVVLTDDEIDQIPVKSTRAIDITQFVDIDEI
                     DPVYFKKTYYLVPEETGIKAYQLMREALREDDKVGIAKVSFRDKEHLAALRLMGDVIV
                     LDTMYWPDELRAQDFEELDAKSEVRPKEVQMARSLIENLTEPWNPEQYGDEYREALLE
                     IVEKKVAGEEIEVITEEEAPEKVVDLMEALQKSVEATKTEKKPARKRAAKKQAAS"
     gene            5940..6782
                     /locus_tag="VF097_02580"
     CDS             5940..6782
                     /locus_tag="VF097_02580"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX6261715.1"
                     /translation="MRWDLLPDISAFDSARRKEAYRRLARSVRGGRQAPTLLDLDEVR
                     ARLGVFEESYDGIRAIPVAHIVGTAGRSRDFDGDFLPRDERTRERWRNVEQTFPRGDF
                     PPIVAYEVDGRYFVVDGHHRVAVAKQRGVDYVDAEVTRLRTRRPLPEDADVGALIIAE
                     QERRFLEEAGLDDLDPPVHIPMSSPVGYPQLLELVKVHGFHLSGERGEVVSLREAGRD
                     WYERVYLPAMEAVRETGLRDALPGAREGDLFLWVYDRHRALRPEDPEAGLAEAARRIL
                     EERR"
     gene            6810..7148
                     /locus_tag="VF097_02585"
     CDS             6810..7148
                     /locus_tag="VF097_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016654695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX6261716.1"
                     /translation="MKLVTAIIKPHKLDEVKDALREIGVEGMTATEAQGFGRQRGHTE
                     IYRGAEYQVDFVPKVKIEIVTEDARAQEVVETIQRTARTGKIGDGKIFVIEAEQVIRI
                     RTGERGPDAL"
     gene            7258..9591
                     /locus_tag="VF097_02590"
     CDS             7258..9591
                     /locus_tag="VF097_02590"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX6261717.1"
                     /translation="MDGAVRELAEGIEGRVAIVALGGYGRRELTPGSDVDLMLLHDGV
                     EGLEDASSRLFYPFWDAGIPLGHSVRTVAECQDLVEDLDVATSLLDARLVAGHEAVLE
                     DLTEGLLARIGRDRGAFLASVAESVEARRERLGSCAWLLEPDLKASGGGVWDVAAVGW
                     VLKALQVEDPVQVGLLRARDVEALDDAREFLIRLRSGLHLETGRRGDRLLLEHQPAIA
                     EAFGFGPEPGMSPPDVLMRSLFEHARSVEHVLRLLLDRAARWVEGGEQEEEARPGSPE
                     EVLDLLALAAERERLPSPATLDRLERAVPARVTWTPAMRAALVRLLAAPGAVDALEAM
                     DRGGILMRLLPEWAAVRCRPQRDPYHRFTVDLHLIHTAGGVAALLRGGAEDPMTAEAV
                     SAIRDREALLLGALLHDIGKRGEGPHVPAGVRVVEPILERLAVAPETADRVRFLVAEH
                     LLLSDTATRRDLTDENLVLDVAGRVGDGGRLASLFLLTTADAEATGPHASTPWRRALV
                     RDLVGKVQRALDHPEEQPDELATIAERLERMAALLAGEHPDPVAAYVDRLPRPYVLAV
                     APEKAARHFRLLEPPPAAGEIRTDVLEGERPGVYELAVAAADRPGLLADIAGALALQG
                     LTILSAQAFTSADGIALDLFAVQGVFEPEVTEERWRRFRGDLRRALEGRVSLERRVEE
                     KRRHYPPPALVEPEVRVDDRVSDFSTVVEVSAGDRLGLLFDLSLAFRDLGLTVHVAKV
                     ATFGPRVVDVFYVRDLEGRKVGKRAEEIREAVLARLG"
     gene            complement(9592..9909)
                     /locus_tag="VF097_02595"
     CDS             complement(9592..9909)
                     /locus_tag="VF097_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010530012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261718.1"
                     /translation="MDTLTATVNAIFAAAVVAILGWIVRGGFRDVHRRIDDLERRFTD
                     RFEQVDRRFEQVDKRFEQVDKRFEQVDRRFEVVEERLNQIRSDISALAFRLVGPPRAE
                     GEP"
     gene            9958..10596
                     /locus_tag="VF097_02600"
     CDS             9958..10596
                     /locus_tag="VF097_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018777512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEX6261719.1"
                     /translation="MTRTIDTPKGSVSALLEGPEEGPLLVLAHGAGNDMRTPLFDGMA
                     RGLSSRSVAVLRFNFPYKEQGRRAPDPERTLREAWLAAFEYGAALRDVVWVGGKSLGG
                     RIASLVVADGLAAAGIVFVGYPLHPPGKPERIRDAHLPGIDVPMLFLQGTKDPFARWE
                     LLEPLVARLPDATLHPVDGGDHSFRVRGQPKDDAATGRTLGEVAGDFIRERS"
     gene            10596..10859
                     /locus_tag="VF097_02605"
     CDS             10596..10859
                     /locus_tag="VF097_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004600793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261720.1"
                     /translation="MPRRHRRDRTGPPPLRPIGAVTPPGEHVEGWSVRFSSKGGATCP
                     YCNRPIQDGEQHVVAWPEDQTDYRRHWHRGCWGREKRRGGPTL"
     gene            10856..11884
                     /locus_tag="VF097_02610"
     CDS             10856..11884
                     /locus_tag="VF097_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX6261721.1"
                     /translation="MRPWSEELVGRFDERTIESEALKGNPLGDPHERPIWVYLPPGYD
                     GDPERRYPAIYEIQGYTGQLDMWRNRMPWRRTFPELLDRMYAEENPPPSAIVVFVDCW
                     TSLGGSQYLDSPGTGRYHTYLCEEVVSWVDAEYRTLADRNHRGIAGKSSGGYGAMVTP
                     MLRPDLFGGFATHAGDALFEVCYWPEFREAARYLRDHYGGSYEAFLEDFRSRPAFSKK
                     QDGTLIELYGYAACYSADEDGTVRLPFDTDTGRLIPEVWERWLAWDPVRMAPERADAL
                     RSMRAIYIDAGKRDEPYLDLGAEAFRRELEAIGVTDYFFEIFDAGHMGIEYRYPLSVR
                     YLAERLSD"
     gene            complement(11857..12915)
                     /locus_tag="VF097_02615"
     CDS             complement(11857..12915)
                     /locus_tag="VF097_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020547654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2578c family radical SAM protein"
                     /protein_id="HEX6261722.1"
                     /translation="MRWDNLRLEAVPSDDRLTAPLFEQGAVARTFDTPEFRGMTFYEV
                     HARSIINRVPEASRVPFRYTINPYRGCSHACRYCFARKTHTYLDLDHGVDFDTKIVVK
                     VNAAQLLRRELSAPRWSGEHIAMGTNVDPYQRAEGRYRLMPGIIEALRDFANPFSILT
                     KGTLILRDLDLLREASEVTEVGTNFSVGFVDDELSRLVEPGTPRPSSRLESVRRLNEA
                     RIPCGVLMAPILPFLTDDDEHLEATVAAIAGAGATHVSPIVLHLRPGAREWWLAWIER
                     ERPDLLPRYRKLYGRGAYAPKVYQAEIGEKVRALARRHGVGRNAPREARNPRRIPEEP
                     PQPEQLKLDAQSESRSAR"
     gene            12984..13355
                     /locus_tag="VF097_02620"
     CDS             12984..13355
                     /locus_tag="VF097_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261723.1"
                     /translation="MEIEVALRRENGGWTALASDPPVSVRAESREDCVRRVRDAVLAR
                     RPNGSDPVTVVVEVAPDLVGVAEAASILGWDKRRVITYVDRGSFPEPVARLAGGRVWA
                     RADVEAFRLAFLARQARRRRR"
     gene            13396..13851
                     /locus_tag="VF097_02625"
     CDS             13396..13851
                     /locus_tag="VF097_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261724.1"
                     /translation="MLRSERGGGSVLTIATILLGVALAIGFPVWMLIRGTQMQAESAG
                     VGVRQAEDVQADALLTGAAGVAHSYFATQGSFQGFSPQAAAALEPSFRWNADPVASPG
                     MVSIRGVTATSVALVTLGSGRPLCLAIDDGQTRRGTTDARSAADCTGGS"
     gene            13879..>14294
                     /locus_tag="VF097_02630"
     CDS             13879..>14294
                     /locus_tag="VF097_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX6261725.1"
                     /translation="MSEAHPAHVDVLRRVPLFADLDGEALRDIAGKAAEVEVPRGHVL
                     LERGQEGAGLFVVLSGTAEVLVGESRIEAGEGEILGELALLVPGLRHTARVQAGEGFR
                     ALAVRRDDFESLLEDHPRMAVAMLRVLARRLAETLS"
BASE COUNT         2022 a         4861 c         5321 g         2090 t
ORIGIN      
        1 ggccgccgcc gcggaggtcg aggccctgga ggccgaggag gaccccgccg cgcgcgagcc
       61 catcgcccgt cggctcgagg acgaggtggc gaagcgggcg cgcgaggtcg cgtcgtacct
      121 cgagccgtcg ctgaccaccc cattcgccgt ggcctgcgtc cccgacgcag cctacgcggc
      181 gtgccggaag gcgcatgcgg tcgccttccg gcacggggtg gtcatcgtgg cctactctgc
      241 ggcgctgccg gtcctgctct ccctgtacgc gcttggatcg cggtacggac agagcggcga
      301 cgtcgaggcc tgcctggcgg agctggagag cgtgctcgag ggcatggagc agaccctgga
      361 gaaccgggtc gctcgcgcca cgacgatgct ccagaacgcc gccgacgagt ggcgagccgg
      421 gctgggtcgg gctcgctggg cggtggcaaa ggggcggggg accgcgggag ccctcgagga
      481 cgagcggcgg ctcgaggcgg tggagtagcg gcggcgatat cctgcgagcc gtgtcgttcg
      541 aggacgcgct gcgggatctg ggattcgtct tctcccgacg ggggaggggc ggggagacct
      601 gggagcaccg tgccacgccg tacctcaccc actacgtgca actcctcgag gacggcaccg
      661 cgctcttcac ttgggaggtc gcgatcgggg agctgatggc ggcccacggg ctccaggtgg
      721 gctccgacga gcacctcaac acgttcctct tccctcgcga ggacgcccgg ggcccccgcg
      781 acgcggcgtg ggtctcgtcc cagatggatg ccgtcgagga gctcctggcg gccatctcgt
      841 tgctcgcggc ccagccctga gcccgtcgct cgcgctacgc ttggcgggtg gagatcgagc
      901 gcgccctcgt cgccttcgcc cacccggacg acgccgagtt cctctgcggt gggacggtgg
      961 cgctctgggc caagcagggg gtcgaggtca cgtacgtctg cgccaccgac ggctcggccg
     1021 gctggaacgc gcccggcatg acccgcgagg agatcgccgc gcgccgcgag gaggagatgc
     1081 gcgaggtggg cgacctcctc ggggtgacgg aggtccgctt cctcggatac ccggacggga
     1141 tgctcgagcc ctcgctcgac ctgcgccggt cgctgacccg ggaggtgcgc cgggcccgcc
     1201 ccgacgtcct cgtctcgttc gacccatcgg agctgtggag cggccgcagc tacatcagcc
     1261 atcccgatca ccgggcggtg ggggaggccg tcctcgccgt cacggcatgc gacgcgccga
     1321 cccgccccca gttccccgag ctcctggaag agggcttgga gccgttcaag gtccgccgcg
     1381 tgtggctcgc ctccgacgag ggcgagcaca tagtggacat cggcgaggcg atcgacctga
     1441 aggtccaggc ggtgcaggcc caccagagcc agatggagaa catgggcgag ttcgacgccg
     1501 acgcccgcgt ccggaagtgg gcgcgcgacc aggcggaggg cacgccccac gagttcgtgg
     1561 agcggttccg cgcgttccgg atcgaggtct gaggatggcc gacgaggaag cgcgccccct
     1621 ggatcccgac gagcgccgga acatcgaggc cgacctcgag gacctatcgg cgatgcggtc
     1681 cgtcttcgag gggcagggca tgcggggcgt cgccatcgcc tgccccgaat gccagaccga
     1741 tcacttctac gggtgggacc tgatcatcga gtccctggag cacatgctcg agaccgggga
     1801 gccgcggatg cacgagcccg cgttcgaccc ccgtgaggag gactacgtcc tctgggacta
     1861 tggcaagggg tacgtggacg cgctggccga cgccggtctc aaggagaccc ccggctccga
     1921 ggtgaccctc ctggcctgcg cctggtgcga cacccccgtg gaggcgcact tctcgtactg
     1981 cccgaagtgc ggccgctcca tggcgccggc ccgcctcctc cgcgagctcg tgagccgggg
     2041 gatggacgag cgcgaggcgc gggccctgct ggtcaaggcc ggcttcgacc cgttccggta
     2101 gcgacgagcg ccgcgagccc gcgccgaccc gagcgcatca tcctggcgtg gttccagcgg
     2161 aagagcggac gggcgatcgg ggcgagcctt cgcatcagtg gcttcctcgc ctcgacctcc
     2221 tgctcgaaga ggagccgggt cgagccgtcg ccgtctggac gtagccggaa cctggcgaag
     2281 ccccggaggt ccccctcgat ccccgcctcg aggacggtct cggtgtcgcg ccggcgggtc
     2341 agcaggaggt gcaggtcgta ggggagcgcg gagcggacac ggacgaccgc gcggtcctcg
     2401 tccacgcggc ggctctcgcg gatctcgggc caccagcggg tccaggcgtc gagctcgtgg
     2461 aggaggtcga ggacgacctc gggcggggcg gggatgcgcc agaccgagcg gaagcggtac
     2521 cggttcaggc tcagggggcc tcctcgagga tgcagtcctg cggcgccttg tcggggcgga
     2581 ggcccttgaa ggagggggcg cgcagcttgc cggccttcgt cacgtggagg tactccacct
     2641 cgcagacgag ctcgggccgc acccaggtcg cgcccttcac cgaggccagc tcgcggtcgc
     2701 cgatcggggg ctcctcctcg cggatctcgc ggaggcggtc cagaacctgg agggtcagct
     2761 gctcggtgaa gcccgtgccc acctgtccca cccagcggag ctcgtcgccc acgtaggcgc
     2821 cgagcagcag cgccccgaac gacgacgccc gcgagccctc acctcgggtc cagccgagga
     2881 tcacgcagtc ctgccgcttc accgccttca ccttccccca gtccttcgac cgccggcccg
     2941 gctggtagcg gctgccgagc ttcttggcca cgatgccctc gtagccgagg cccttcgccg
     3001 cctcgaacag ccgctccccc tcgccgtcga ggtagagggt gagccccagg cgatccgatt
     3061 cggtgacgat cccctcgagc aggcggcggc gctcctccag cggctcgttc gtcaggtctc
     3121 gctcgtcgag ccacagcagg tcgaaggcgt acagcctcac ggggatgcgg cggcgggcct
     3181 tctcgatgtc ggacggagcg gagaggttga tacgctcctg gagccgctcg aacgacggcc
     3241 ggccccgctc gtccacggcc acgatctcgc cgtcgaggac ggcccacagc gcgttcgtcc
     3301 agcgggccag gttgtcgagc tcggggtact gggccgtctg gtcccgaccc ttccgcgaga
     3361 ggagccgcgt ggcgtcggtg gtgacgtagg cgagcgtccg gacgccgtcg agcttgggct
     3421 cgaaccgcca cccggggtcg tcgaacgggt cgtggccggg gtcggcgagc atgggctcga
     3481 acgccggcgg cggctcgggc tggggcgcat ccgacccctt gcccaggaag atcagccagt
     3541 cgcggtcggt cttcacgagg tggtactcgc cgcggtgccg cgtgccatgg aggcggaagg
     3601 agaccttgtc gtcggtccag tccagcggct cgtaggtgcc gcggtcgaag atgcggacct
     3661 cgccggcccc gtagtgaccc tcggggatcc atccctggaa ctccccgtag tcgagggggt
     3721 ggtcctcggt gtggacggcg aggcgacggt cgccgggctt caaggggagg ccgcgaggga
     3781 ccgcccagga gaccagcacc ccgccctcct ccagacgcag gtcgtagtgg aggcgggtgg
     3841 cttcgtgctt ctggatcacg aaggagcgcc cgtcgccgcc ggcggggacg ggcgggggct
     3901 cgggcgtctc cgcgaagtcg cgcatctcgc cgtacttggc gaggcgggcc gcggggtccc
     3961 cgtcgtcggt tttcggatcg gcggtgagct cctccgacgc gggctcaccc tccccagcgg
     4021 tcggagcccc ggtcgcctcc gcttcctcaa cgcgcgggtc catccccagc gcctcgagcg
     4081 ctgggcgcag gtccacgggc tcctccagga ccccgcggaa caggtcgccc acctcggcca
     4141 gccgcttgtg catcgtgacg atcgtgaagt caccgggctc gatgccctcc tcgacctcgt
     4201 cccacgtcac cggggccgac accgtggccc ccggctgagg acgcaccgag tacgcggcgg
     4261 cgatgttcgc cccctgccgg ttcatgttgt ggtcgatgaa gaccttgccc gcgcgcttgg
     4321 agacctccca ctccatcgtc acgcggtcgg ggtcggcctt caggatcagc cgccccaccg
     4381 cgccgacgaa gtccctgacc tgctggtagc ttggcccgag ctcgatcggc acgtagatct
     4441 gcatcccggt cgcgcccgag gtcttcgggt aggaggtgag cccgagcgcc tcgagggccg
     4501 cgcggacgtg ccgggccacc gcccgaacgt cgccgaacgt gatcggctcg aacggatcga
     4561 ggtcgaagaa gaggtagtcg ggccggtcga cgctctcgca cctggagtgg agcgggtgga
     4621 actcgatcgc ccccaagttg gccacgaaca ggaggcccgc ctcgtcctcg acccggaggt
     4681 actcgatcgt cttcgagccc tcctcgtcga gcacggggca gcgcggcatc cactcggggg
     4741 tgtgggacgg ggcgttcttc tcgtagaact cgcctccctc gatcccgtcc ggcatccggc
     4801 gcatcgtgag gggcctcccc cgcaggtagg ggaggaccgt gtcggcgacg ttccagtagt
     4861 aggcgagcag gtcgcccttc gtgtagccgt cgtcgggcca gtaggccttg gggaggttcg
     4921 agaggcgcac gcggcggccg gccgcctcca cccaccagtg gtctccctcc cgctcagctt
     4981 cgagcctccg gggagtctct acggtgaagg gctcgccgcg cgccatgccc cgatggtagg
     5041 gcctggccgc catcccggcc gctcgccggg tatcctgggc cggcgagccg agaacaggga
     5101 ggtcgtgatg ccgaggacca tgtggaaggg ggccatctcc ttcggcctcg tgtcgatccc
     5161 cgtgcgggtg ttcccggcga cggaggagaa gaccctccgc ttcaaccagc tgcacgacga
     5221 ggaccacgga cgcatcaagt accagcgggt ctgcgcgaag tgcggcaagg aggtcccgta
     5281 cgagaacatc gtcaagggct acgagtacga gaaggaccgc tacgtcgtcc tgaccgacga
     5341 cgagatcgat cagatcccgg tgaagtccac acgcgccatc gacatcacgc agttcgtgga
     5401 catcgatgag atcgacccgg tctacttcaa gaagacctac tacctggtgc ccgaggagac
     5461 cggcatcaag gcctaccagc tgatgcggga ggcgctccgc gaggacgaca aggtcgggat
     5521 cgccaaggtc tcgttccgcg acaaggagca cctggccgcg ctgcggctga tgggcgacgt
     5581 gatcgttctc gacacgatgt actggcccga cgagctccgg gcccaggact tcgaggagct
     5641 cgacgcgaag agcgaggtac ggcccaagga ggtccagatg gcccgctcgc tgatcgagaa
     5701 cctcaccgag ccctggaacc ccgagcagta cggcgacgag taccgcgagg cgctcctcga
     5761 gatcgtggag aagaaggtgg ccggcgagga gatcgaggtc atcaccgagg aagaggcgcc
     5821 ggagaaggtc gtcgacctca tggaggcgct ccagaagagc gtcgaggcca cgaagacgga
     5881 gaagaagccg gcgaggaagc gcgcggcgaa gaagcaggcc gcctcctgag gggccggccg
     5941 tgcggtggga cctcctgccc gacataagcg ccttcgacag cgccagacgc aaggaggcct
     6001 accggcgtct ggcgcgaagc gtccgcgggg ggcgtcaggc ccccacgctg ctcgatctgg
     6061 acgaggtccg cgcgcggctc ggcgtcttcg aggagtcata cgacgggatc cgggccatac
     6121 ccgtggctca catcgtcggc accgccgggc ggtcccgcga cttcgacggc gacttcctgc
     6181 cacgcgacga gcgaacgcgc gagcggtggc gaaatgtcga gcagacgttc ccgcggggtg
     6241 acttccctcc gatcgtggcc tacgaggtgg acgggcgata cttcgtcgtg gacggtcacc
     6301 accgcgtggc ggtcgccaag cagcgcggcg tggactacgt ggacgcggag gtgacgcgcc
     6361 tccggacccg ccgcccgctg ccggaggacg cggacgtggg cgcgctgatc atcgcggagc
     6421 aggagcggcg gttcctggag gaggcggggc tggacgacct cgatccgccg gtgcacatcc
     6481 cgatgtcgag cccggtcggg tacccgcagc tgctcgagct ggtcaaggtc cacggcttcc
     6541 acctctccgg cgagcggggg gaggtcgtgt cgctgcggga ggcaggccgc gactggtacg
     6601 agcgcgtcta cctcccggcg atggaggccg tgcgggagac ggggctgcga gacgccctcc
     6661 ccggggcccg ggagggcgac ctcttcctgt gggtctacga tcgtcaccgg gcgctgcggc
     6721 ccgaggatcc ggaggccggg ctcgcggagg cggcacgccg gatcctggag gagcggcggt
     6781 aggatgctcg ggcacgcgag gagggacgca tgaagctggt gacggcgatc atcaagccgc
     6841 acaagctcga cgaggtgaag gacgccttgc gtgagatcgg ggtcgagggc atgacggcta
     6901 ccgaagctca gggcttcggc cgtcagcgcg gccacaccga gatctaccgc ggggccgagt
     6961 accaggtcga cttcgtcccc aaggtgaaga tcgagatcgt gaccgaggat gcgcgggctc
     7021 aggaggtcgt ggagacgatc cagcggacgg cgcggaccgg gaagatcggc gacgggaaga
     7081 tcttcgtcat cgaggccgag caggtgatcc ggatccgcac gggagagaga ggccccgacg
     7141 cgctctgatc gggcgaccgg cccgatcgac cggctcgagc gtgggctacg ctcgctggag
     7201 gaggcctact cgagcggcca ccacgggggc tggtcggcga ggcgacggac cgagctcgtg
     7261 gacggggccg tccgggagct ggccgagggc atcgaggggc gcgtcgcgat cgtggcgctc
     7321 ggaggctacg gtcgcaggga gctgacgcca gggtcggacg tcgacctgat gctccttcac
     7381 gacggcgtcg aggggctcga ggatgcgtcc tcccgcctct tctatccgtt ctgggacgcg
     7441 gggattccgc tggggcactc ggtccggacc gtggcggagt gccaggatct ggtcgaggac
     7501 ctcgacgttg ccacgtcgct cctcgacgcg aggctcgtgg cgggccatga ggcggtgctc
     7561 gaggacctca ccgaggggct gctggcgagg atcgggcggg accggggggc cttcctggcc
     7621 tcggtcgccg agagcgtgga ggcgcgtcgc gaacggctcg gctcgtgcgc atggctgctc
     7681 gagccggacc tcaaggcgag cggaggcggc gtatgggacg tggcggcggt ggggtgggtg
     7741 ctgaaggccc tgcaggtgga ggacccggtc caggtgggcc tgctgcgggc ccgcgacgtc
     7801 gaggccctgg acgacgcccg cgagttcctg atccgcctgc ggagcgggct gcacctcgag
     7861 acgggtcggc gcggcgaccg tctgctcctg gagcaccagc cggcgatcgc cgaggcgttc
     7921 ggcttcggtc ccgagccggg gatgagcccg cccgacgtgc tgatgcgatc cctgttcgag
     7981 cacgcccgat ccgtggagca cgtcctccgg ctcctgctcg accgggcggc tcgctgggtg
     8041 gaggggggcg agcaggagga ggaggcccgg cctggctcgc ccgaggaggt gctggacctg
     8101 ctggcgctgg ccgcggagcg agagcggctc ccgtcgccgg ccacgctcga ccggctggag
     8161 cgggccgtcc ccgcgcgggt gacgtggacg ccggcgatgc gcgcggccct cgtccgcctg
     8221 ctggcggcac ccggtgcggt cgacgcgctc gaggcgatgg accgaggcgg gatcctgatg
     8281 cgacttctgc cggagtgggc cgcggtccgg tgccgcccgc agcgcgatcc ctaccaccgg
     8341 ttcacggtgg acctccatct gatccatacc gctgggggcg ttgcggcgct gctgcggggc
     8401 ggggccgagg atccgatgac ggccgaggcg gtctccgcga tccgggatcg cgaggccctg
     8461 ctgctgggcg cgctgctgca cgacatcggg aagcggggcg aggggccgca cgtcccggcc
     8521 ggggtccgcg tggtggagcc gatcctggag cggctcgccg tcgcgcccga gaccgcggac
     8581 cgggtgcgct tcctcgtggc ggagcacctc cttctgtcgg acacggcaac gcgccgagac
     8641 ctcaccgacg agaacctcgt gctcgacgtg gccgggcggg tgggcgacgg ggggcgactg
     8701 gcctcgctgt tccttctcac gacggcggac gccgaggcga ccggcccgca cgcttcgacg
     8761 ccctggcgcc gggctctcgt gcgcgatctc gtggggaagg tccagagggc gctcgaccat
     8821 cccgaggagc agcccgacga gctggcgacg atcgcggagc ggctggagcg gatggcggcc
     8881 ctgctggcgg gcgagcaccc cgatccggtg gccgcctacg tcgatcgcct gccgcggccc
     8941 tacgtcctcg ctgtggcccc cgagaaggcg gcccgccact tccggctgct ggagcccccg
     9001 ccggcggcgg gcgagatccg gaccgacgtc ctggagggcg agcgccccgg cgtctacgag
     9061 ctggccgtcg ccgcggcgga ccggccgggc ctgctggccg acatcgccgg ggcgctcgcc
     9121 ctccagggcc tgacgatcct ctcggcgcag gccttcacga gcgcggacgg gatcgccctc
     9181 gatctgttcg cggttcaggg cgtgttcgag ccggaggtca ccgaggaacg ctggcgccgg
     9241 ttccggggcg acctgcggcg cgcgctcgaa ggacgggtct cgctggagcg gcgtgtggag
     9301 gagaagaggc gccactaccc gccgccggcg ctagtggagc cggaggttcg ggtggacgat
     9361 cgcgtctcgg acttctccac cgtggtagag gtgtcggccg gcgatcgcct cggcctcctc
     9421 ttcgacctct cgctcgcctt ccgcgaccta gggctcaccg tgcacgtggc gaaggtcgcc
     9481 accttcggtc cgcgcgtcgt ggacgtcttt tacgtgcggg atctcgaggg gcggaaggtg
     9541 gggaagcggg cggaggagat ccgtgaggcc gtcctggctc ggctcggcta gctagggctc
     9601 tccttcggct ctcggggggc ccacgagacg gaacgcgagg gccgagatgt cggaccggat
     9661 ctggttgagc cgctcctcga ccacctcgaa cctccggtcg acctgctcga atcgcttgtc
     9721 cacctgctcg aatcgcttgt ccacctgttc gaacctccgg tcgacctgct cgaaccggtc
     9781 ggtgaagcgc cgctcgaggt cgtcgatgcg gcggtggacg tcccggaacc cgcccctgac
     9841 gatccacccc aggatggcga cgaccgccgc ggcgaagatc gcattgaccg tggccgtcag
     9901 tgtgtccatg cagctccttc ccgggggagg atgccgggct caggctacca tcgcggggtg
     9961 acgcgaacga tcgacacccc caaggggtcg gtctcggccc tgcttgaggg gcccgaggaa
    10021 ggcccgctgc tcgtcctggc ccacggagcc gggaacgaca tgcggacgcc gctcttcgac
    10081 gggatggccc gcgggctctc ctcgcggagt gtggccgtcc tccgcttcaa cttcccctac
    10141 aaggagcagg gccgccgggc cccggatccc gagcgcacgc ttcgggaggc ctggctggcc
    10201 gccttcgaat acggcgctgc gctccgtgac gtcgtctggg tggggggcaa gtcgctgggc
    10261 ggaaggatcg cctcgctcgt ggtggcggac gggctcgccg ccgcggggat cgtcttcgtt
    10321 ggctatcccc tccacccccc ggggaagccc gaacggatcc gtgacgccca cctccccggc
    10381 atcgacgtcc caatgctgtt cctgcagggg acgaaggatc cctttgcgcg gtgggagctg
    10441 ctggagccgc tggtggcgcg gctccccgac gcgaccctgc acccggtgga cggcggagac
    10501 cactccttcc gggtccgcgg gcaacccaag gacgacgcgg cgaccggacg gacgctcggc
    10561 gaggtcgccg gggacttcat ccgggagcgg agctgatgcc gcgccgccac cggcgcgacc
    10621 ggaccggccc gccgccgctc cgaccgatcg gcgccgtcac gccccccggc gagcacgtcg
    10681 aggggtggag cgtccggttc tcgagcaagg gcggcgccac ctgtccatac tgcaaccggc
    10741 cgatccagga cggggagcag cacgtcgtgg cctggcccga ggatcagacg gactaccggc
    10801 ggcactggca ccgcgggtgc tggggccggg agaagcggag aggagggccc acgctgtgag
    10861 gccgtggtcg gaggagctcg tggggaggtt cgacgagcga acgatcgaga gcgaggcgct
    10921 gaagggcaat ccgctgggcg atccgcacga gcggcccatc tgggtgtacc tgccgcccgg
    10981 ctacgacggc gacccggagc gccgctaccc ggcgatctac gagatccagg gctacaccgg
    11041 tcagctcgac atgtggcgga accgcatgcc gtggcgccgt accttccccg agctgctcga
    11101 ccgtatgtac gcggaggaga acccgccccc atccgcgatc gtcgtcttcg tcgactgctg
    11161 gacgtcgctc gggggcagcc agtacctcga ctcgccgggg accggccggt accacaccta
    11221 cctctgcgag gaggtcgtct cctgggtcga cgccgagtac cggacgctgg ccgatcgcaa
    11281 ccaccggggg atcgcgggga agtcgagcgg cggctacggg gcgatggtga cccccatgct
    11341 gcggccggac ctgttcgggg gcttcgccac gcacgccggc gacgccctgt tcgaggtctg
    11401 ctactggccg gagttccgcg aggccgcccg atacctgcgc gaccactacg gcggctccta
    11461 cgaggccttc ctcgaggatt tccgctcccg tccggccttc tccaagaagc aggacgggac
    11521 gctgatcgag ctgtacgggt acgcggcctg ctactcggcg gacgaggacg gcacggtgcg
    11581 gctccccttc gacaccgaca cgggccgcct gatccccgag gtctgggagc gatggctggc
    11641 ctgggatccc gtccggatgg cgcccgagcg ggccgacgcg ctgcggtcga tgcgcgcgat
    11701 ctacatcgac gcggggaagc gcgacgagcc gtacctcgat ctcggcgccg aggccttccg
    11761 ccgggagctg gaggcgatcg gagtcaccga ctacttcttc gagatcttcg acgcggggca
    11821 catggggatc gagtaccggt acccgctctc ggtgcgctac ctggccgagc ggctctcgga
    11881 ctgagcgtcc agcttgagct gctcgggctg cgggggctcc tctgggatgc gcctggggtt
    11941 gcgcgcctct cgcggagcgt tccttccgac gccgtgccgc cgggcgaggg cccggacctt
    12001 ctcgccgatc tcggcctggt acaccttggg ggcgtaggcg cctcgcccgt acagcttccg
    12061 atagcgaggg aggaggtcgg gccgctcgcg ctcgatccag gccagccacc attcgcgggc
    12121 gccggggcga agatggagga cgatcgggga cacgtgcgtc gccccggcgc ccgcgatcgc
    12181 ggccaccgtg gcctcaagat gctcgtcgtc gtcggtgagg aacgggagga tcggcgccat
    12241 cagcacgccg caggggatcc gggcctcgtt cagcctgcgg acagattcga gtcgagatga
    12301 cggcctcggc gtccccggct ccaccagcct cgacagctcg tcgtccacga atcccaccga
    12361 gaagttcgtc cccacctcgg tgacctccga tgcctcccgc agcaggtcga ggtcgcgcag
    12421 gatcagcgtc cccttcgtga ggatcgagaa ggggttggcg aagtcccgga gcgcctcgat
    12481 gatccccggc atcagccggt accgtccctc ggctcgctgg tacgggtcca cgttcgtccc
    12541 catcgcgatg tgctcgccag accaccgcgg cgccgacagc tccctccgca gcagctgggc
    12601 cgcgttgacc ttcaccacga tcttcgtgtc gaagtccacg ccgtggtcga ggtccaggta
    12661 cgtgtgcgtc ttccgagcga agcagtagcg acaagcatga ctgcatcctc tgtaggggtt
    12721 gatcgtgtag cggaagggga cgcgggaggc ctcgggcacc cggttgatga tcgagcgcgc
    12781 gtgcacctcg tagaaggtca tcccgcggaa ctccggcgtg tcgaaggtcc tggccaccgc
    12841 cccctgctcg aagaggggcg cagtgagtcg gtcgtccgag ggaactgcct cgagtcggag
    12901 gttgtcccac cgcatgggac ggcaaggata gaacacgtgt tcgattcggg caagcgtgcc
    12961 gatgatcgta tgatgccgtc gtcgtggaga tcgaggtggc gctccgccgg gagaacggag
    13021 gctggacggc cttggcctcc gaccccccgg tctccgtccg agccgagtcc cgagaggact
    13081 gcgtgcgtcg agtccgggac gcggtgctcg cccgccgccc caacggcagc gatcccgtca
    13141 cggtggtcgt cgaggttgcg cccgatctgg ttggcgtggc ggaggccgct tcgatcctgg
    13201 gatgggacaa gcgccgggtg atcacctacg tggaccgggg ctccttcccg gagccggtgg
    13261 cacgtctggc cgggggccgg gtgtgggcac gggccgacgt cgaggcgttc cgcctcgcct
    13321 tcctcgcacg gcaggcccgg cgccggcgcc gctgaccctc ttcaggaccg cgccgacagg
    13381 gtcgatacct ggggcatgct tcggtcggag cgcgggggcg gcagcgtctt gacgatcgcc
    13441 acgatcctcc tgggggtggc gttggcgatc ggattccccg tgtggatgct gatccggggg
    13501 acccagatgc aggccgagag cgcgggcgtc ggggtgcggc aggccgagga tgtgcaggcg
    13561 gacgcgctcc tgaccggcgc cgcaggggtg gcccactcgt acttcgcgac gcagggctcg
    13621 ttccagggtt tctccccgca ggcggcggcg gcgctcgaac cctcgttccg ctggaacgcg
    13681 gacccggttg cgtcgcccgg gatggtcagc atccggggcg tgaccgcgac ctcggttgcc
    13741 ttggtcaccc tgggctcggg gcggccgctc tgcctcgcga tcgacgacgg tcagacccgc
    13801 agagggacga ccgacgcccg ttcggccgcc gactgcaccg gcggatcctg accggcgccc
    13861 gcggtgtagt cttcgcgcat gagcgaggct catcccgccc acgtggacgt cctgcgccgc
    13921 gtcccgctgt tcgccgatct cgacggtgag gcgctccgcg acatcgcggg gaaggccgcg
    13981 gaggtcgagg tgccccgggg ccacgtcctg ctggagcgag gacaggaggg tgcggggctc
    14041 ttcgtcgtgc tgagcgggac ggcggaggtc ctcgtggggg aatcccggat cgaggcgggt
    14101 gagggcgaga tcctcggcga gctcgcgctg ctcgtcccgg gcctccgcca cacggctcgc
    14161 gtccaggccg gggagggctt ccgggccctg gcggtgcgcc gcgatgactt cgagtcgctg
    14221 ctcgaggatc acccgaggat ggccgtggcg atgcttcggg tgctggcccg gcgcctggcc
    14281 gagaccctct ccta
//
LOCUS       DASUTZ010000054         3262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1439591, whole genome shotgun sequence.
ACCESSION   DASUTZ010000054 DASUTZ010000000
VERSION     DASUTZ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439591"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..139
                     /locus_tag="VF097_02635"
     CDS             <1..139
                     /locus_tag="VF097_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008424849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bL28 family ribosomal protein"
                     /protein_id="HEX6261726.1"
                     /translation="SHSHRRSPRRWSPNVQKVRVLVNGAPRRLHVCTKCLKAGKVQRA
                     V"
     gene            complement(136..969)
                     /gene="thiD"
                     /locus_tag="VF097_02640"
     CDS             complement(136..969)
                     /gene="thiD"
                     /locus_tag="VF097_02640"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX6261727.1"
                     /translation="MAESDDSRRRGGITLGEVRTALTIAGSDSGGGAGIQADLKTFTA
                     LGVFGTTAITAVTIQNTKGVEGYEELSPETVGGQIRAVATDIGVHAAKTGMLASAGII
                     EAVADALVETRVPNLVVDPVFVSKHGHPLLQDDAVEALRTRILPLATLVTPNLPEAAG
                     LTGLQVEAEQDMRRAGEAILELGAGAVLVKGGHLSGPEATDLLVTGDGAAPFSSPRVD
                     TPHTHGTGCVLSAAIAARLALGDELQDAVRGGKEFVTGAIRGGLEIGEGIGPVDPAWR
                     S"
     gene            complement(920..1915)
                     /gene="thiL"
                     /locus_tag="VF097_02645"
     CDS             complement(920..1915)
                     /gene="thiL"
                     /locus_tag="VF097_02645"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX6261728.1"
                     /translation="MATEDELVRAIKKLLSGEQPGVVVPIGDDAAVVEPGGHHLVVTV
                     DTLVERVHFDPDVAQPDDLGYKAVVVNVSDVAAMGGSPRYAVASLAVPPDTRPAWVIE
                     LYGGMRAAVEDHGMALVGGDTVRADQCVLTVTVLGTVAEGRAVTRSGARPGDRIVVTG
                     SLGAAAGGLRLVQGREGATRRAVMTDPGRQLVAALDRPVARVGEGEALAQAGATSMID
                     VSDGLALDLSRVCEASGVGARVSLPDVPVAEALEELAEEIGVDPLDLALHGGEDYELV
                     ATLPGEAVEDARARLLDRFGTPLTDIGEIIETGLVAVGEDGRERRLEAKGWDHFG"
     gene            2013..2222
                     /locus_tag="VF097_02650"
     CDS             2013..2222
                     /locus_tag="VF097_02650"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX6261729.1"
                     /translation="MPVINTLDLEGVSEKSWGDAAREALKEAAKTIRHINRLEVLDYG
                     TEVEDEKIARYRTHVRIYFQVESER"
     gene            2325..>3262
                     /locus_tag="VF097_02655"
     CDS             2325..>3262
                     /locus_tag="VF097_02655"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF013461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEX6261730.1"
                     /translation="MIEAKRVHDNPSPEELRAFTEEMPSCRITEYDNPNVQTRAVSRS
                     AASTYVATDDPSITDSQTITREEYARIVAMQDEYIREQEMLVIDGFIGNDPGFRTRAR
                     LLIERAYANIAGMQQKLYYPPERDGEPEVTVIYTPNLSAPGYPDDRIIAVDLEAGVTR
                     ILNSDYFGESKKGGLRMWNKLVYDRGGLSLHAGLKMIPVDGERRVGLIIGLSGTGKTT
                     TTFTNQNDSLPAQDDFIALMPGGLVYGTENGCFAKTFSLDPEYEPAIWSAVTKPSAYL
                     ENVYQDEQGKVNFFETSYTQNGRAVFGLEDLGDYADP"
BASE COUNT          512 a         1246 c         1064 g          440 t
ORIGIN      
        1 gagccactcc catcggcgct ccccgcgccg gtggagcccg aacgtgcaga aggttcgggt
       61 gctcgtgaac ggcgctcccc gccggctcca cgtgtgcacg aagtgcctca aggccggcaa
      121 ggtccagcgc gccgtctagg agcgccaggc cgggtccacc ggcccgatcc cctcgccgat
      181 ctccagcccc ccacggatcg cccccgtgac gaactccttg ccacctcgca ccgcgtcctg
      241 gagctcatcg cccagggcga gccgggccgc gatcgccgct gaaagcacgc agccggtgcc
      301 gtgcgtgtgc ggcgtgtcga ccctcgggct cgagaacggc gccgctccgt ccccggtgac
      361 cagaaggtcg gtggcctcgg gccccgacag gtgtccgccc ttgacgagca ccgcccccgc
      421 cccgagctcg aggatcgcct cgcccgcccg gcgcatgtcc tgctcggcct cgacctggag
      481 cccggtgagg ccggccgcct cggggaggtt cggcgtgacc agcgtggcca gggggaggat
      541 gcgcgtccgc agggcctcga ccgcgtcgtc ctggagcagg ggatggccgt gcttcgagac
      601 gaagaccggg tcgaccacga ggttgggcac ccgcgtctcg acgagggcgt cggccactgc
      661 ctcgatgatg cccgccgacg ccagcatccc cgtcttggcc gcgtgcacgc cgatgtcggt
      721 ggccaccgcc cggatctggc cacccaccgt ctcgggcgac agctcctcgt agccctcgac
      781 ccccttcgtg ttctggatgg tgaccgcggt gatcgcggtc gtcccgaaca cgcccagcgc
      841 ggtgaaggtc ttgaggtcgg cctggatccc ggcaccgccc cccgagtccg agccggcgat
      901 cgtcagcgcc gtgcggacct cacccaaagt gatcccaccc cttcgcctcg agtcgtcgct
      961 ctcggccatc ctcccccacc gcgaccaggc ccgtctcgat gatctccccg atgtcggtca
     1021 ggggcgtccc gaaccgatcg agcagcctcg cccgcgcgtc ctccacggct tcgccgggca
     1081 gggtcgcgac gagctcgtag tcctctcccc cgtgaagggc gaggtccagg gggtccacgc
     1141 cgatctcctc ggccagctcc tccagcgcct cggcgaccgg cacgtcgggc agcgacacgc
     1201 gggccccgac cccgctcgcc tcgcacacgc gcgagaggtc cagcgccagc ccgtccgaga
     1261 cgtcgatcat cgaggtggcc ccggcctgcg ccagcgcctc gccctcgccc accctggcca
     1321 ccggacggtc cagggcggcc acgagctggc gccccggatc cgtcatgacc gcgcgcctcg
     1381 tcgccccctc ccggccctgc acgagccgca ggccgccggc cgccgcgccc aggctcccgg
     1441 tgaccacgat ccggtccccc ggccgggctc ccgacctggt cacggcccgc ccctcggcca
     1501 cggtccccag gaccgtcacg gtcaggacgc actggtccgc ccggaccgtg tcccccccga
     1561 cgagggccat cccatggtct tcgaccgccg cccgcatgcc cccgtagagc tcgatcaccc
     1621 acgccggcct ggtgtcgggc ggaacggcga gcgaggcgac cgcgtagcgg gggctcccgc
     1681 ccatggccgc cacgtcggag acgttgacga ccacggcctt gtagccgagg tcgtcgggct
     1741 gggccacgtc gggatcgaag tggacgcgct ccacgagcgt gtccacggtc acgaccagat
     1801 ggtgcccgcc cggctcgacg accgcggcgt cgtccccgat ggggacgacg acccccggct
     1861 gctcgcccga gagcagcttc ttgatggcgc ggaccaactc gtcctctgtg gccacggctt
     1921 cccttcctcg gtccctctcg gggataatcc tagaggcgcg ggcccgctcc ggccccgccc
     1981 cgaaggacga ccccgtcgag aggagacgag acatgccggt gatcaacacg ctggacctcg
     2041 agggcgtttc cgagaagtcg tggggcgacg ccgcccgcga ggcgctcaag gaggcggcga
     2101 agacgatacg acacatcaac cgcctggagg tgctcgacta cggcaccgag gtggaggacg
     2161 agaagatcgc gcgctaccgg acccacgttc gcatctactt ccaggtcgag tccgagcggt
     2221 agttcgagga gcgccccgag cgggtacacc cccggagcgc gcctgggggg cccgctccgc
     2281 tgggtgtacc atgggcgtcc ccgacatcgc cgtaggagga ggccgtgatc gaggcgaaga
     2341 gggtccacga caatccgtct cccgaggagc tccgcgcgtt caccgaggag atgccgagct
     2401 gccggatcac cgaatacgac aacccgaacg tacagacccg cgccgtctcg cgcagcgcag
     2461 ccagcaccta cgtcgccacc gacgatccct cgatcaccga ctcccagacg atcacccgcg
     2521 aggagtacgc gcgcatcgtg gcgatgcagg acgagtacat ccgcgagcag gagatgctcg
     2581 tgatcgacgg cttcatcggg aacgaccccg ggttccgcac cagggccaga ctcctgatcg
     2641 agagggccta cgccaacatc gcggggatgc agcagaagct ctactatccg ccggagcgtg
     2701 acggcgagcc cgaggtcacc gtgatctaca cgccgaacct ctccgccccc ggctaccccg
     2761 acgaccgcat catcgcggtg gatctcgagg ccggcgtcac gcggatcctc aactcggact
     2821 acttcgggga atcgaagaag ggcgggctgc ggatgtggaa caagctcgtc tacgaccgcg
     2881 gcggcctctc gctccacgcg ggcctcaaga tgatcccggt ggacggggag cggcgtgtgg
     2941 gcctcatcat cgggctctcg gggaccggca agaccacgac gaccttcacg aaccagaacg
     3001 actccctccc cgcccaggac gacttcatcg cgctgatgcc gggcgggctc gtctacggca
     3061 ccgagaacgg ttgcttcgcc aagaccttct cgctggaccc cgagtacgag ccggcgatct
     3121 ggagcgccgt caccaagccc agcgcgtacc tcgagaacgt gtaccaggac gagcagggca
     3181 aggtgaactt cttcgagacc tcctacacgc agaacggacg agcggtgttc gggctcgagg
     3241 acctcggcga ctacgccgac cc
//
LOCUS       DASUTZ010000055         6409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1050701, whole genome shotgun sequence.
ACCESSION   DASUTZ010000055 DASUTZ010000000
VERSION     DASUTZ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6409
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050701"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="VF097_02660"
     CDS             complement(<1..860)
                     /locus_tag="VF097_02660"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX6261731.1"
                     /translation="MGAIARLRDSGAWRDVFEGEAGRRVGALTRQTPVVRAPGMLVKA
                     EGLQRTGSYKARGAIAKLATIPAGGEVVTASAGNHGLGVAYASAALGHRATVVVSEAA
                     PEVKRRGIEGLGARLVVHGAGYDDAEAYARSLAEGEAALFVGYADDHVIVGNGGTIAQ
                     ELLEQVEGLGEGDAVVIPVGGGGLASGIGAALAGSGVGLVGVEPETNRAMSISFDEGA
                     ARTSYPEGASTLAEGLEGAVAENTYELCRESLDEIVLVGEEEIRRAIARAYTEMGLVL
                     EASAAVAPAAV"
     gene            complement(865..1260)
                     /locus_tag="VF097_02665"
     CDS             complement(865..1260)
                     /locus_tag="VF097_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261732.1"
                     /translation="MGWEDLGGQLTSVAAAAWWGDGEVQVFAVHDDGEVWNRYWDGRR
                     WHDWESLGGSFTGHPAAAARDGSQIDVFAVGADGVLRHRWWDGVRWVDWEDVEGAPHD
                     GSAVSCAWVGDRLHLLVTGPDGRLWHATL"
     gene            complement(1308..2105)
                     /locus_tag="VF097_02670"
     CDS             complement(1308..2105)
                     /locus_tag="VF097_02670"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX6261733.1"
                     /translation="MDRLRFGTYLAPALLPVYEAITEHVGERVGIDTELVVETSYDSC
                     ENDENEVCFVCSLPYVMLERRGIDVALPVAAPVVADPRYEGRPIYYSDVIVSADAHFR
                     SFDDLRGRSWAYNEPLSHSGYGITRFHLLTMGETNGFFDRVVEAGFHEEAIRMVARGE
                     VDGSAIDSHLLAVELRSEPELAERLRVVASLGPSTIQPVAVSRRVPPDLRDAIRETLI
                     TMHWDLGMRRRLGESLIERLVPVDASSYDDIRMMVDVCEQAGFLQLR"
     gene            complement(2135..3622)
                     /locus_tag="VF097_02675"
     CDS             complement(2135..3622)
                     /locus_tag="VF097_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261734.1"
                     /translation="MSPTPLGWRSLRQTSRRSERPGRLLGLTLTVVLLSFACTGGLVV
                     APGGTPPATRGEFAELPGSFVLIGQEPLFERGMNAGLAVWRDFAYVGNRTDGSSGHPH
                     PGILVVTVEDPGAPEVVGEIRRLHVADPGATSRELRIWPGGELLIALTFGCDAVLHGC
                     APDLVRPSFSFYDIGGDRAARPRFVSEYQPQGQPHKFYLWQDPRDRGRALLFVSTPTP
                     IGENLLVVDISGARSGRVRELATWSAEFPDPGPDDALHSLSVSIDGRRAYLAHLTAGF
                     LILDTSELARGAEEPQIRTITPLDQPARWPGVGPHSAVKVPGRDLVLTTDEVYGRSAG
                     GGCPWGWGRLISIAEETSPEVISEFGVSPYNDLSYCEGVSSERDAAASFSSHNPTVTE
                     HLALISWHGAGLQAFSTEDPAAPERVAEFLPDPLLTVATEDPPLSSGPDKVVMWSYPI
                     ILNGLIYVVDVRNGLYVLEYEGPFDEELAEVSFLEGNSNLGDASP"
     gene            complement(4715..5308)
                     /locus_tag="VF097_02680"
     CDS             complement(4715..5308)
                     /locus_tag="VF097_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261735.1"
                     /translation="MLIDRFLPRYDFALAHAVVLPASSEECWAAARRVDFLRHPVFRV
                     LLAGRALPLRVAEVVTRAQRASGTAAEALHAFRLFDMPRLGFVLLGEEPGVEVVYGQV
                     SRPWQAAASEGPPIDPAGFTEFRTPGFAKIVFSLSVISRGGGSSILVVETRVALTDDD
                     SSRRFRRYWRVIRPFSDVLRRIVLRMIAAEVRRSRAD"
     gene            complement(5648..5836)
                     /locus_tag="VF097_02685"
     CDS             complement(5648..5836)
                     /locus_tag="VF097_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007298843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261736.1"
                     /translation="MGKGSAGAAAGGGAIYGLGIFGALFYYWQQADAFWEYIWAIFPK
                     AILWPAFMVYEAFRALGA"
     gene            complement(5951..6037)
                     /locus_tag="VF097_02690"
     tRNA            complement(5951..6037)
                     /locus_tag="VF097_02690"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6001..6003),aa:Leu,seq:gag)
     gene            complement(6068..>6409)
                     /locus_tag="VF097_02695"
     CDS             complement(6068..>6409)
                     /locus_tag="VF097_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261737.1"
                     /translation="QEGEQEAFCLVWVADAPTAQAILDPLFDPDSPDNRTGIEDEDLA
                     RLLARARATESAQEREELYARAERRALELMPLVPLAWFRSNVAAQDYVTGLEVDPLGR
                     FDVAALAFDEG"
BASE COUNT         1028 a         2387 c         2102 g          892 t
ORIGIN      
        1 acggcggcgg gcgcgaccgc ggccgacgcc tccagcacga gccccatctc cgtgtacgca
       61 cgggcgatcg ctctgcggat ctcctcctcg cccaccagca cgatctcgtc cagcgactcc
      121 cggcacagct cgtaggtatt ctcggccacc gccccctcca ggccctcggc cagcgtggag
      181 gccccctccg gataggaggt cctggcggcc ccctcgtcga acgagatgct catggcgcgg
      241 ttcgtctctg gctcgacgcc cacaagcccg accccgctcc ccgccagcgc cgccccgatc
      301 cccgaggcca gccctccccc gcccaccggt atcacgaccg cgtcgccctc gcccagcccc
      361 tcgacctgct ccaggagctc ctgcgcgatc gtccccccat tgccgacgat cacgtggtcg
      421 tcggcgtacc cgacgaagag ggcggcctcg ccctcggcca gcgagcgggc gtacgcctcc
      481 gcgtcgtcgt agccggcgcc gtggacgacg agccgcgcgc cgagcccctc gatcccacgc
      541 cgcttcacct cgggagccgc ctccgagacc accacggtgg ccctgtggcc aagcgcggcc
      601 gacgcgtagg cgacgcccag tccgtggttg ccggccgagg ccgtcacgac ctccccgccc
      661 gcggggatcg tggcgagctt ggcgatcgct ccccgggcct tgtaggaccc cgtgcgctgg
      721 aggccctccg ccttcaccag catcccgggg gcccggacca cgggggtctg tcgcgtgagc
      781 gctccgacgc ggcgcccggc ttccccctcg aacacgtcgc gccaggcgcc gctgtcgcgg
      841 agccgggcga tcgctcccat gcgatcagag ggtcgcgtgc cagaggcgcc cgtccggacc
      901 ggtcaccagg aggtggaggc gatccccgac ccacgcgcac gagacggccg agccgtcgtg
      961 cggcgccccc tcgacgtcct cccagtcgac ccagcggacg ccgtcccacc agcgatgacg
     1021 gagcaccccg tcggcgccca cggcgaagac gtcgatctgc gacccatcgc gcgcggcggc
     1081 ggccgggtga ccggtgaagc tcccccccag cgactcccag tcgtgccacc ggcggccgtc
     1141 ccagtaccgg ttccagacct cgccgtcgtc gtggacggcg aagacctgca cctcgccgtc
     1201 gccccaccag gccgccgcgg cgaccgaggt gagctgccct cccagatcct cccagcccat
     1261 cgcgccctcc cggaagaacc ctatcgctcc cccgaacccg acgcaggtca gcgaagctgg
     1321 aggaagccgg cctgctcgca gacgtcgacc atcatgcgta tgtcgtcgta ggacgaggcg
     1381 tccacaggca cgagccgctc gatcaggctc tcccccagcc tgcgtcgcat cccgaggtcc
     1441 cagtgcatcg tgatcagcgt ctcccggatc gcgtcccgga ggtccggcgg cacccgcctc
     1501 gagacggcca ccggctggat cgtcgacggc ccgagagacg cgacgaccct cagacgctcc
     1561 gccagctcgg gctcgctgcg cagctccacc gcgagcaggt gcgagtcgat cgccgacccg
     1621 tccacctcgc cccgggccac catccggatc gcctcctcgt ggaacccggc ctccacgacc
     1681 cggtcgaaga agccgttcgt ctcgcccatg gtcagcaggt ggaagcgggt gatgccgtag
     1741 cccgagtggg agagcggctc gttgtacgcc cacgatcgcc cgcggagatc gtcgaacgat
     1801 cggaagtgcg cgtccgcgga cacgatcacg tccgagtagt agatcggccg cccctcgtag
     1861 cgggggtcgg ccaccaccgg cgcggcgacc gggagcgcga cgtcgatccc ccgccgctcg
     1921 agcatcacgt agggcaggct gcagacgaag cagacctcgt tctcgtcgtt ctcgcaggaa
     1981 tcgtaggacg tctccaccac gagctcggtg tcgataccca cccgctcgcc cacgtgctcc
     2041 gtgatcgctt cgtacacggg gagcagcgcc ggcgccaggt aggtcccgaa ccgcaggcga
     2101 tccatgcccc gagtctaggc ctggaaccgg cagactaggg tgaggcgtct cccaggttgg
     2161 agttgccctc caggaacgag acctcggcga gctcctcgtc gaagggtccc tcgtactcga
     2221 gcacgtagag cccgttccgc acgtcgacga cgtagatcag gccgttcagg atgatcgggt
     2281 agctccacat cacgaccttg tcgggccccg agctgagggg cggatcctcg gtcgccaccg
     2341 tgagcagggg gtctgggagg aactccgcaa cccgttccgg cgcggccgga tcctccgtgg
     2401 agaacgcctg cagccccgcc ccgtgccagc tgatcagcgc gagatgctcg gtgacggtcg
     2461 ggttgtgcga cgagaaagac gcggcggcat cccgctccga ggagacgccc tcgcagtacg
     2521 agagatcgtt gtatggggag acaccgaact ccgagatcac ctcgggagag gtctcctcgg
     2581 cgatgctgat gaggcgaccc catccccacg ggcatccgcc gccggccgat cgaccgtaga
     2641 cctcgtcggt cgtgaggacc aggtcgcgtc cggggacctt caccgcgctg tgcgggccca
     2701 cgcctggcca ccgggccggc tggtccaggg gcgtgatcgt ccggatctgc ggctcctcgg
     2761 cgcctcgagc cagctccgaa gtgtcgagaa tcaggaagcc cgccgtcaga tgcgccaggt
     2821 aggcgcgccg gccgtcgatc gagaccgaca gcgagtgcag ggcatcgtcg gggccgggat
     2881 ccgggaactc ggccgaccat gtggcgagct ctcggacccg cccactcctc gccccggaga
     2941 tgtccacgac gagcaggttc tcgccgatgg gggtgggagt ggagacgaag agcagagcgc
     3001 gtcctcggtc cctcggatcc tgccagaggt agaacttgtg gggctgaccc tggggctgat
     3061 actcggagac gaaacgtgga cgggcggctc gatcgcctcc gatgtcgtag aagctgaagg
     3121 acggccggac caggtccggc gcgcatccgt ggaggacggc gtcgcatccg aacgtcagcg
     3181 cgatcagtag ctcgccgccg ggccagatcc gcagctctcg tgacgtcgct cccggatccg
     3241 ccacgtgcag acggcggatc tcccccacca cctcgggagc gccgggatcc tccacggtga
     3301 ccacgaggat ccccgggtgc ggatgcccgg atgagccgtc cgtccggttg ccgacgtacg
     3361 cgaagtctcg ccacacggcg agccctgcgt tcatcccgcg ctcgaagagg ggctcttgac
     3421 cgatgaggac gaaggacccg gggagctcgg caaactctcc tcgggtcgcg ggcggggtgc
     3481 cgccgggagc gaccacgagc cccccggtgc acgcgaagga gaggagaacg accgtgagcg
     3541 tcaggccgag cagacgcccg gggcgctcac tcctccgact cgtctgccgc aggctcctcc
     3601 aacccagagg ggttgggctc accgagccac gcctcatccc ccgcgctctc gccctgggcc
     3661 gctcgctctt cccgccgccg ggcgcgcgcg agacgacgtt cttccctctc agaggccttc
     3721 ctggccttca cggccttccg ctgtcgcttc ccataggtct ccggcatacc ccccaccgta
     3781 tcgcacccgc gtcgctccgg gtcgcaaccg atgcgcggtt ccaccgacgg cgtcggcagg
     3841 cccctcgagc agccgtagcc cgccgtcctg gcgaggtgca cgaccgtcgc atacagtcga
     3901 gggaggctgt ggacacgacc gcaggggagg atctgggaca tggccgatcg gcgagcaccc
     3961 ctcgaaaccc tcagcgagtt cccgctcatg cacgcgttgt tcggccggag ggcccgacgc
     4021 ttcgggttgg ggatggagat cccctccggg ccgttggcct tcagctcgcg tcggcgttcg
     4081 cgttgcaggt cgccgggggt gtggtcgatc ccccgggccg ctccgaaccc aaccgctcgg
     4141 ccgcgagagt gcccgagagt ggccagaagt gtgagattat gccgcactac gcttcatgcc
     4201 tgagcgcagg cggaccggca gggtgtgacc aagtgtgacc acaccgacaa cgctcgagcc
     4261 gggaacggga tgcgcgtgcg atcaccacct cggcaaggca gcgaagaacg ccgccgagac
     4321 ctgaccgacg cacacgaccc cggccccaat cggccgccag caggaagggg gctggccgga
     4381 tgaggtggtc acagccgtgt tgtggctggc gagcgacgcg gcatcgaacg tgaacggtgc
     4441 aacgctgggc gtcgatggcg tatggcctgc cgcctgaggg actctcgcca cgacgaggac
     4501 tcgactggag gcaggaactc ctccttcgcg tgcgccgaac cgcagcccct cccgcagtca
     4561 gccagccacc gcaagagagc cgacatcgac gcttgcgccg ggacctcgaa gggtgcaggt
     4621 atctcctcgg aaccgctgga gccttcgggc tccggtggtt ccatcctttc ccccgcctct
     4681 tggtcagcgt cgcgtacgcg agcggccgcg cgcctcagtc ggcgcgggag cgacgcacct
     4741 cggccgcgat catgcgcagg acaatacgcc ggagtacgtc gctgaacgga cggatcaccc
     4801 gccagtagcg gcggaaccgg cgcgaactgt catcgtccgt caacgcgacg cgtgtctcga
     4861 cgacgaggat cgacgagccg ccgccacggg aaatgacgct aagggagaag acgatcttcg
     4921 cgaaccccgg cgtgcggaac tccgtgaatc ccgccggatc gattggcggc ccctcggacg
     4981 ccgccgcctg ccacggccgg ctgacctgcc cgtacacgac ctcgacgccc ggctcctctc
     5041 ccaggaggac gaagccgagg cgcggcatgt cgaacaaccg gaacgcgtgc agcgcctccg
     5101 cggccgtacc cgacgcacgc tgggcccgcg tcacgacctc ggccacgcgc agcggaagcg
     5161 ctctccccgc gagcagcacg cggaagaccg ggtgccgcag gaaatcgact cgccgcgccg
     5221 ctgcccagca ctcctccgaa gacgccggaa gaacgacggc gtgcgccagg gcgaagtcgt
     5281 agcgcggcag gaaccggtcg atcagcacgt cagtctctcc cctgcgcgcc gccccgcgct
     5341 tgcctgagga acgcggtgcc ccgcagacgg tttcagaggc ctgcgaacag ctcaccactg
     5401 cagtgaccac ccgaggcaac catagcagtc gtacgacacc gccctggtcg tcgatgaagc
     5461 cgggcaccta catcggtggt gcagccgtcc gcaatcgccg cccgggtggt ggcctccgcc
     5521 gaaccggtga cgtgtctcga gcacggacag actctctccg cgatgacgat gcggcgaaca
     5581 aaggggcgtt gttagccgaa gcaccaggtc aggcaggtgg cggccgaggc gcctcctaga
     5641 ctggccccta tgcgccgagg gccctgaacg cttcgtagac catgaacgcc ggccagagaa
     5701 tggccttcgg gaagatcgcc caaatgtact cccagaacgc gtcggcctgc tgccagtagt
     5761 agaaaagcgc gccgaagatc ccgagcccgt agatcgctcc gccacccgcg gcggcgcctg
     5821 cgctgccctt ccccatagcc gccctcctcc ctcgtcggtg ctgcacgagg ctatatcgag
     5881 cacctcaccg agtcgagcat gggtgtggga gggcgttcta tccggccttc gcgtttctcc
     5941 tggtagtcgg tgtcggaggg gagagtcgaa ctcccacggg gtggttagcc ccactaggcc
     6001 ctcaacctag cgcgtctgcc agttccgcca ctccgacgac catccgatta tagcgacggg
     6061 cgggccctca accctcgtcg aaggccagcg cggcaacatc gaagcgtccc agcggatcca
     6121 cctcgaggcc cgtgacgtag tcctgggccg ccacgttcga tcggaaccag gccaacggca
     6181 cgagcggcat cagctcgagg gcccgtcgct ccgctcgcgc gtacagctcc tcgcgctcct
     6241 gggccgactc ggtggcccgg gcccgcgcca gcagccgcgc gaggtcctcg tcctcgatcc
     6301 ccgtccggtt gtccggggag tcgggatcga agagcggatc caggatcgcc tgcgcggtcg
     6361 gcgcgtccgc cacccacacc aggcagaagg cctcctgctc cccctcctg
//
LOCUS       DASUTZ010000056         2867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1828986, whole genome shotgun sequence.
ACCESSION   DASUTZ010000056 DASUTZ010000000
VERSION     DASUTZ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1828986"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VF097_02700"
     CDS             <1..568
                     /locus_tag="VF097_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX6261738.1"
                     /translation="HSYPDEHPIVAFEDVRVPAANLIGEEGDGMSFTHEWFRYERLMI
                     AARCCGAADRLIEEAEGFARERMAFGKPIAEYQGVAFPLADSIVELWAARLMTYRTAR
                     GMDEGADVKLLHAHCSMTKLYASEMANRVADRAVQIFGGRGYMRENVAERFFRELRVD
                     RIWEGTSEVQRAIIADQLGKRGTGIAEG"
     gene            573..1016
                     /locus_tag="VF097_02705"
     CDS             573..1016
                     /locus_tag="VF097_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018530610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6261739.1"
                     /translation="MEWRLEVVFVPVSDVDRAKAFYRDAVGFHEDFDTRFNDDLRMVQ
                     LTPPGSGCSIALMSGAAAGRPGPPDIEPGSLQGLMVVVDDLTAARERLLEHGVEVSEV
                     LEVVHEEGGSVYRPVTGEPIGWNAYAFFQDPDGNGWTLQASSPEG"
     gene            complement(1019..2440)
                     /locus_tag="VF097_02710"
     CDS             complement(1019..2440)
                     /locus_tag="VF097_02710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6261740.1"
                     /translation="MRGRDIPASADRSYWYREAQAHAPLEPAAPLQGEVEADVLIVGG
                     GYTGLWTAWHLTEREPDARVVLLERDLCGAAASGRNGGFLTGWWDDLPDMAARYGMEP
                     AVAACLALDESIEAIGRWIENRGADVWLARAGYLAASSSPAQDGLWREATEAAAVAGH
                     PDAYREVTGDEVREICDSPALRGGAFMPAGASVQPARLGLALRAALLERGVRIHEHTH
                     VRRLQTKEPVVAETEGGRVRARRAVVATNAWAAGWRALRGRLVVRGSYVLVTEPAPER
                     LEVIGWTGGEPITDLRAALFYFRTTPDGRIAFGGAGRAGLGSKVGPRYDHDPVSLDHV
                     YAGFRRFFPSFEGVGITEAWGGAVDVAALHHPVVGTLRGGAVHHATGYSGNGVAPSHL
                     MARALAALALEVEDESLTLPMVGAKAKRFPPEPFRSVGAAIVQSAIMRRERLEDEGRR
                     VGWFTRLLSTMPRRMGYALGRSD"
BASE COUNT          398 a         1006 c         1060 g          403 t
ORIGIN      
        1 ccactcctac cccgacgagc acccgatcgt ggccttcgag gacgtgcgcg tgcccgccgc
       61 caacctgatc ggggaggagg gcgacggcat gtccttcacc cacgaatggt tccgttacga
      121 gcgcctgatg atcgcggccc ggtgctgtgg ggcggccgac cggctgatcg aggaggcgga
      181 ggggttcgcg cgggagcgga tggccttcgg gaagcccatc gccgagtacc agggggtggc
      241 gttcccgctg gccgactcga tcgtggagct gtgggcggcg cgcctcatga cctaccggac
      301 cgcgcggggg atggacgagg gggcggacgt gaagctgctg cacgcccact gctcgatgac
      361 caagctctac gcgtcggaga tggcgaaccg ggtcgccgac cgggcggtcc agatcttcgg
      421 gggccgcggc tacatgcgcg agaacgtggc cgagcggttc ttccgggagc tacgggtgga
      481 ccggatctgg gaggggacct cggaggttca gcgggccatc atcgccgatc agctgggcaa
      541 gcgcgggacg ggcatcgcgg aggggtgaag gggtggagtg gcggctcgag gtcgtgttcg
      601 tccccgtgtc cgacgtggac cgtgccaagg cgttctaccg tgacgcggtc gggttccacg
      661 aggacttcga cactcgattc aacgacgacc tgcggatggt ccagctgacc ccgcccggct
      721 ccggatgctc gatcgcgctg atgtcggggg cggcggcggg gcggcccggg ccgcccgaca
      781 tcgagccggg ctcgctccag ggactgatgg tcgtcgtcga cgatctcacc gcggcgcggg
      841 agcgcctcct cgagcacggc gtcgaggtca gcgaggtcct ggaggtcgtc cacgaggagg
      901 gcggctccgt ctaccgcccc gtcacgggag agccgatcgg gtggaacgcc tacgccttct
      961 tccaggatcc cgacggcaac ggctggaccc tccaggccag ctctcccgag ggctagcttc
     1021 agtcgctgcg gcccagcgcg tatcccatgc gccggggcat cgtggagagc aggcgcgtga
     1081 accatcccac gcgccgcccc tcgtcctcga ggcgctcccg ccgcatgatc gcggactgca
     1141 cgatcgccgc gcccacggac cggaagggct cgggggggaa gcgcttggcc ttggccccga
     1201 ccatggggag cgtcagcgac tcgtcctcga cctcgagcgc cagcgcggcg agggcccggg
     1261 ccatcaggtg cgagggcgcc accccgttgc ccgagtagcc cgtcgcgtgg tggacggcgc
     1321 cgccccggag cgtgcccacc acgggatggt ggagggcggc gacgtccacc gcgccgcccc
     1381 aggcctcggt gatcccgacc ccctcgaacg aggggaagaa gcggcggaag cccgcgtaga
     1441 cgtggtccag cgagaccggg tcgtggtcgt agcgggggcc gaccttcgag ccgaggccgg
     1501 cccgccccgc gcccccgaag gcgatccgcc cgtcgggcgt ggtgcggaag tagaagaggg
     1561 cggcccgcag gtcggtgatg ggctcgccgc cggtccaccc gatcacctcg aggcgctccg
     1621 gggccggctc ggtgaccagc acgtagctgc cccggacgac gagccggccc cggagcgccc
     1681 tccatcccgc cgcccacgcg ttcgtggcca cgacggcccg ccgggcccgg acgcgccccc
     1741 cctcggtctc ggcgacgacc ggctccttcg tctggagccg gcgaacgtgc gtgtgctcgt
     1801 ggatccggac cccccgctcc agcagggcgg cgcggagggc gagccccagc ctcgccggct
     1861 ggaccgaagc cccggccggc atgaaggcgc cgccgcgcag ggcgggggag tcgcagatct
     1921 cgcggacctc gtccccggtg acctcgcggt aggcgtcggg atggccggcc acggccgcgg
     1981 cctccgtggc ctcgcgccag aggccgtcct gggccgggct cgaggaggcc gcgaggtagc
     2041 ccgccctcgc gagccatacg tcggccccgc gattctcgat ccaccgcccg atcgcttcga
     2101 tgctctcgtc gagggccaga cacgcggcga ccgcgggctc catcccgtag cgcgccgcca
     2161 tgtcggggag gtcgtcccac cacccggtga ggaagccccc gttccgcccg ctggccgcgg
     2221 ccccgcagag atctcgctcc agcaggacga cccgcgcgtc aggctctcgc tcggtgaggt
     2281 gccaggccgt ccagagtccc gtgtacccgc ctccgacgat caggacgtcg gcctcgacct
     2341 cgccctgcag cggcgccgcg ggctcgagcg gtgcatgtgc ctgcgcctcc cggtaccagt
     2401 agctccgatc ggcgctcgcc gggatgtctc tccctcgcat cgctggagag tctacgctgg
     2461 gattgaacgg tggcagtggg atcgtcctac gagcaggggg cagcagcatg gggtgaccga
     2521 tcagctttgg tgttgctgag atgagccgcg acgaggccta ctacgagaat ctcatagcgc
     2581 gagttcgcgg tgtgctcatc gaagtcatgg accacctgcc agcccggacg gcgagtctga
     2641 ggggcggctc gacctggagg cgtcgagccg cggaatagcg aatggagatg gttgcctccc
     2701 tcctccagat gggttggggg ttggcgccgt tgggtccgcg cggcgaaact gtgcgccgtg
     2761 tcgaagcaga tcaggattcc cctgcgagga actggtggcg agccggtgga cctccaccgc
     2821 acgctcgtct cgcacgggac gttctcgctg ccgcccgcgc tcctcga
//
LOCUS       DASUTZ010000057         4702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1440426, whole genome shotgun sequence.
ACCESSION   DASUTZ010000057 DASUTZ010000000
VERSION     DASUTZ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440426"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1419
                     /locus_tag="VF097_02715"
     CDS             <1..1419
                     /locus_tag="VF097_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX6261741.1"
                     /translation="EVADAPYLHRGLNLADMAHVLVLLEQGIAPPEPAQRLLRLLLEV
                     HGTSHEDFPYDPSLGDAYNSRERFFIERLGTDAGWLHAGRPRREAGRVALRICLRDLV
                     VSLVSDAAGFAESLADAAEEHADVVMPDQTYLQQAQPSTFGHYLLTFAGPSLRETDRM
                     LLEDDWVNRSPGGAGCVNGSRLAVDRDRTASLLGFRGVIEHTRDAMWQTDGLVALLSG
                     STSLVSNLSKLAEDLEIWDSQEFDFVELAGPYTRSSILMPQKRNPYALSIVRGASGVL
                     IGRLSGFLAVVKTPSARSDNLIFAYGEVPRALELAGRVTRLMRGVVATMRPHPERMWE
                     SLTQGFSQATDLAEHVMQTAEVDYRTAYLVVGEAIQRAAREGLRGMDVTGELLDEAAK
                     DVVGRPLGLAERDLSDVLDPRAIVRTRTSRGGSAPEVVREMAAGFRADAERVRAEAAG
                     RAEAYARAEADLLSLARRTAEG"
     gene            complement(1388..1819)
                     /locus_tag="VF097_02720"
     CDS             complement(1388..1819)
                     /locus_tag="VF097_02720"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX6261742.1"
                     /translation="MEVTAYWEGGYTTRVPIRDFEIRIDEPERYGGRDTGPMPTEMLL
                     GTLAACFAMAFHHAAKKHDVELPDLEVSARGEYGNLRFDRIAVEVRTSHPDREQLEGF
                     LEKAIDYCYVTNTLAGGPELEYHVAGGEAHEVSPPPSAERG"
     gene            1818..3239
                     /locus_tag="VF097_02725"
     CDS             1818..3239
                     /locus_tag="VF097_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007581485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HEX6261743.1"
                     /translation="MTVESSNSEQLALPEEVRAVNVGLGVFGEAVRAQGGEAVDVEWR
                     IPAGGDRESVEALTGLYGPRAAAIDEANEEVLRRIDGSAPVLVGVGSAGRDVPGMGDR
                     TILHPGPPLPWEEFCDPLRRSVHAAVMAEGWAETPEEAGRLVAGGDVELDAANHHATV
                     LPMATTIGPSAPVLLVESPQGGTRAFAAINQGPGDQQWLGVDRPEAVERLVFLREAAA
                     PVLDEAIRSGDPLDVFSIAAQALQMGDDAHMRSQAATNLTIRFLLPRLLRLDDERAPE
                     VGTFLSENHLFFLTVGMAAAKATMLWAEQVEGSSIVTGMARNGTTFGVRLPGLDEWHV
                     APAPPVEDALFHGDYGPDDAAPDIGDSAILELVGLGGAAAAASPAVAAFVGGTMADAV
                     AATEEMIRIGLGTSSRFKLPFMGFRGSPIGVDVRRVVELEITPSVNTGILHASEGTGQ
                     IGAGVAHAPVECFRDAMLALARA"
     gene            3236..3994
                     /locus_tag="VF097_02730"
     CDS             3236..3994
                     /locus_tag="VF097_02730"
                     /inference="COORDINATES: protein motif:HMM:NF022834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HEX6261744.1"
                     /translation="MTSARFIGGGAREVLGSGELGQVIAWFPRACYAAFPAGLLVLVG
                     EGVHPGPLYVGIEGDLPRVPVGDPVGVGMDRVSLGGVEVRVGDRTWRGWTPRPEQMEA
                     AASVAASAAEEAGERSLLDPGARAAAEQALLAGDVEAAARALEGRGPGLTPSGDDALA
                     GILFARRLLGGLGAEHELLPIAERAASSPISRGFATWAARGQALAPAHRLVAASVAGD
                     AGGARRAARVLTLVGESSGADFCAGMALGLRAVA"
     gene            complement(3991..>4702)
                     /locus_tag="VF097_02735"
     CDS             complement(3991..>4702)
                     /locus_tag="VF097_02735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6261745.1"
                     /translation="EEHGIEAAVVPNGVDLARFAAPLDPEAVRDLRRRVGSADRFLFL
                     TVGGIEPRKGTMELVEALADLRAGEGPGPVVAVVGGHSFQDHTPYRERVLARMEELGL
                     ELGRDLVVLGTVPDQELDAWYRAADAFVFPSAKEGFGLVVLEALASRLPVVATDIPVF
                     AEYLTHGIDALLAKAGDAGSLAEQMRRAMLDEELRGRLADAGPELASRYSWEASASRH
                     REVYAEVIRAAAAPAPAG"
BASE COUNT          580 a         1655 c         1823 g          644 t
ORIGIN      
        1 gaggtcgccg acgcgcccta cctgcaccgc gggctgaacc tggccgacat ggcgcacgtg
       61 ctcgtgctgc tggagcaggg gatcgcgccc cccgagccgg cgcagcggct gctgcggctg
      121 ctgctcgagg tccacggcac gtcccacgag gacttcccct acgacccgtc gctgggcgac
      181 gcgtacaact cccgggagcg gttcttcatc gagcggctgg ggaccgacgc tgggtggctc
      241 cacgccggcc gtccccgccg cgaggccggg agggtcgcgc tccgtatctg cctccgggac
      301 ctggtggtct cgctcgtctc ggacgccgcc gggttcgccg agtccctggc cgacgcggcc
      361 gaggagcacg ccgacgtcgt gatgcccgat cagacgtacc tccagcaggc ccagccgtcg
      421 acgttcggtc actacctcct gaccttcgcc ggcccctccc tccgggagac ggaccgcatg
      481 ctgctcgagg acgattgggt gaatcggagc ccgggcgggg ccggctgcgt caacggcagc
      541 cggctggcgg tcgaccgcga ccggacggcc tcgctcctcg ggttccgagg tgtgatcgag
      601 cacacgcgag acgcgatgtg gcagaccgac ggcctggtgg cgctgctctc gggctccacg
      661 agcctggtca gcaacctcag caagctcgcg gaggatctcg agatctggga cagccaggag
      721 ttcgacttcg tggagctggc cggtccctac acgcgatcca gcatcctcat gccccagaag
      781 cggaacccat acgcgctgtc gatcgtccgc ggcgcctcgg gggtgctgat cgggcggctc
      841 tcgggcttcc tggccgtggt gaagacgccc tcggcccgca gcgacaacct gatcttcgcc
      901 tacggcgagg tcccgagggc cctggagctc gccgggcgcg tgacgcggct gatgcgcggg
      961 gtcgtggcca cgatgcgtcc ccaccccgag cggatgtggg agtcgctgac gcagggcttc
     1021 tcccaggcca cggacctggc cgagcacgtg atgcagaccg cggaggtcga ctaccggacg
     1081 gcatacctgg tggtgggcga ggcgatccag cgggcggcgc gggaggggct tcgggggatg
     1141 gacgtcaccg gggagttgct cgacgaggcg gccaaggacg tggtggggag gccgctgggc
     1201 ctggccgagc gggacctgtc ggacgtgctc gaccccaggg cgatcgtccg gacgaggacc
     1261 tcgcggggcg gctcggcgcc ggaggtggtc cgggagatgg cggccgggtt ccgggccgac
     1321 gcggagcgcg tgcgggccga ggcggcgggg cgcgccgagg cctacgcccg ggccgaggcc
     1381 gacctgctca gcctcgctcg gcggacggcg gagggctgac ctcgtgcgcc tccccgccgg
     1441 ccacgtggta ctcgagctcc gggcctccgg cgagcgtgtt cgtcacgtag cagtagtcga
     1501 tcgccttctc caggaacccc tcgagctgtt cccggtcggg gtggctggtg cgcacctcga
     1561 ccgcgatccg gtcgaaccgg aggttgccgt actcgccgcg ggccgagacc tcgaggtcgg
     1621 ggagctcgac gtcgtgcttc ttggccgcgt ggtggaaggc catggcgaag caggcggcca
     1681 gtgtgcccag gagcatctcg gtgggcatgg ggccggtgtc acgtccgccg taccgttcgg
     1741 gctcgtcgat cctgatctcg aagtctcgga tcgggacgcg cgtcgtgtat ccgccctccc
     1801 agtaggcggt aacctccatg acggtggagt ctagcaacag cgagcagctc gccctcccgg
     1861 aggaggtccg cgccgtcaac gtcggtctgg gcgtgttcgg cgaggccgtg cgggcccagg
     1921 gcggggaggc ggtggacgtg gagtggcgca tccccgccgg cggcgaccgg gagtcggtcg
     1981 aggcgctgac cggcctctac gggcctcggg ccgccgcgat cgacgaggcg aacgaggagg
     2041 tccttcggcg catcgacgga tcggcccccg tcctcgtggg cgtgggctcg gccgggcgag
     2101 acgtgccggg catgggcgac cggacgatcc tccacccggg ccccccgctc ccgtgggagg
     2161 agttctgcga tcccctgcgc cgctcggtgc acgcggcggt gatggccgag gggtgggccg
     2221 agacgccgga ggaggccggg aggctcgtgg cgggcggcga cgtggagctc gacgctgcga
     2281 accatcacgc caccgtcctc ccgatggcga cgacgatcgg acccagcgcc cccgtgctgc
     2341 tcgtggagag cccccagggc gggacccgcg ccttcgccgc catcaaccag ggcccgggcg
     2401 accagcagtg gctgggcgtg gatcgtcccg aggcggtgga gcggctcgtc ttcctccggg
     2461 aggcggcggc tccggtgctc gacgaggcga tccggtcggg cgacccactg gacgtcttct
     2521 cgatcgccgc gcaggcgctc cagatggggg acgacgccca catgcggtcg caggcggcca
     2581 ccaacctcac gatccgcttc ctcctcccgc gactgctccg tctcgacgac gagcgcgcgc
     2641 cagaggtggg gaccttcctg tccgagaacc acctgttctt cctgaccgtg gggatggcgg
     2701 cggcgaaggc cacgatgctc tgggcggagc aggtcgaggg ctcgagcatc gtcacgggga
     2761 tggcgcggaa cggcaccacg ttcggggtgc gcctgcctgg gctcgacgag tggcacgtgg
     2821 cgccggctcc tcccgtcgag gacgcgctct tccacggcga ctacggtccc gacgacgccg
     2881 cgcccgacat cggggacagc gcgatcctcg agctcgtggg cctgggggga gcggcggcgg
     2941 cggcctcccc ggcggtggcg gcgttcgtgg ggggcacgat ggccgacgcc gtggccgcca
     3001 ccgaggagat gatccgcatc gggctgggga ccagcagccg tttcaagctc cccttcatgg
     3061 gcttccgcgg ctcgccgatc ggcgtggacg tgcggcgggt ggtcgagctc gagatcacgc
     3121 cgtcggtcaa caccggcatc ctccacgcga gcgaggggac cgggcagatc ggcgcggggg
     3181 tggcccacgc gccggtggag tgcttccggg acgcgatgct cgccctcgcc cgggcgtgac
     3241 ctcggcccgc ttcatcgggg gcggcgccag ggaggtgctg gggtccggcg agctgggaca
     3301 ggtgatcgcc tggttccccc gagcctgcta tgcggccttc cccgccggcc tgctcgtgct
     3361 cgtcggggag ggcgtccatc cgggcccgct ctacgtcggg atcgaggggg acctcccgag
     3421 ggtgcccgtg ggggacccgg tgggggtggg gatggatcgg gtctcgctgg gcggcgtcga
     3481 ggtacgggtg ggcgaccgca cctggcgggg gtggacgccc aggcccgagc agatggaggc
     3541 cgcggcgagc gtcgcggcgt ccgcggcgga ggaggcgggg gagcggtcgc tgctcgatcc
     3601 gggggcgcgc gccgccgccg agcaggcgct tctcgccggc gacgtcgagg cggcggcgcg
     3661 cgccctggag gggcgggggc ccggcctgac gccgtcgggt gacgacgcgc tggccgggat
     3721 cctcttcgcc cggcggctcc tgggcggcct cggggccgag cacgagctcc tccccatagc
     3781 cgagagggcc gcgagctccc ccatctcccg gggcttcgcc acgtgggccg cgagggggca
     3841 ggccctggcc ccggcgcatc ggctggttgc ggcctcggtg gccggggacg ccggaggcgc
     3901 ccggagggcg gcccgcgtcc tgacgctcgt gggtgagtcg tctggagccg acttctgcgc
     3961 ggggatggcg ctggggctgc gcgccgtggc ctagcctgcc ggggcgggcg cggcggcggc
     4021 ccggatcacc tcggcgtaca cctcgcggtg gcggctcgcc gacgcctccc agctgtagcg
     4081 tgaggcgagc tccggcccgg cgtcggcgag ccgcccccga agctcctcat ccagcatcgc
     4141 ccgccgcatc tgctcggcca gcgatccggc gtcgcccgcc ttcgccagca gcgcgtcgat
     4201 cccgtgggtg aggtactcgg cgaagaccgg gatgtcggtg gccaccaccg gcagccgcga
     4261 cgccagcgcc tcgagcacca cgagcccgaa cccctccttg gctgacggga agacgaacgc
     4321 gtcggcggcc cggtaccagg cgtcgagctc ctggtcgggg accgtgccaa gcaccaccag
     4381 gtcccgtccg agctccagcc ccagctcttc catccgggcc aggacccgct cgcggtaggg
     4441 ggtgtggtcc tggaacgagt ggccgccgac cacggccacg acggggccgg gcccctcacc
     4501 ggccctgagg tccgcgaggg cctcgaccag ctccatcgtg cccttccggg gctcgatccc
     4561 acccaccgtc aggaagagga accggtccgc cgagccgacg cggcgccgca ggtcgcgaac
     4621 cgcctcgggg tcgagcggcg cggcgaagcg agcgaggtcc acgccgttgg gcaccacggc
     4681 ggcctcgatc ccgtgctcct cg
//
LOCUS       DASUTZ010000058         3340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_274317, whole genome shotgun sequence.
ACCESSION   DASUTZ010000058 DASUTZ010000000
VERSION     DASUTZ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274317"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VF097_02740"
     CDS             <1..264
                     /locus_tag="VF097_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016443178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEX6261746.1"
                     /translation="VDCIYEGGRMLYIQPDECVDCDACEPVCPVTAIFAEDDVPEEWK
                     NFTPVNAEFFTEEIAGYTSPGGAAQVGPVDADHPVVAGWEAPA"
     gene            complement(268..768)
                     /gene="msrA"
                     /locus_tag="VF097_02745"
     CDS             complement(268..768)
                     /gene="msrA"
                     /locus_tag="VF097_02745"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX6261747.1"
                     /translation="MGETQKATFAAGCFWQVEADYRKLDGVVSTEVGYMGGSLDNPSY
                     KDVCTDRTGHAEVTQVEFDPERVSYEDLVELFWTIHDPTQVNRQGPDVGTQYRSAIFT
                     HDEDQALIAKRSLAKAQARFSKPIATEIVPAQAFWLAEDYHQRYLEKRGMASCTIQLA
                     ETDASD"
     gene            962..1741
                     /locus_tag="VF097_02750"
     CDS             962..1741
                     /locus_tag="VF097_02750"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6261748.1"
                     /translation="MSSGSLSFDRAASYYDRTRPISRTALPRVMELLVRELEGRGHCL
                     DMGVGTGIVSIPLAERGIPVIGLDLSRPMLERLREKAGGAAPLPLVQADATAMPFRDG
                     AFGGAVIRHVLHLIPDWERALGELLRVVRPGGVVLVNHGEYPEGLRELFDRFVREARV
                     ERPWVGLGHDELDRVHGWMRSRGAAIRPLEPVREEVEEPLERFVEGMREGHYSWTWEV
                     EDRVRLEAANRVAAWAARELGPLDRPMRREAEIAFWAYDVP"
     gene            complement(1726..>3340)
                     /locus_tag="VF097_02755"
     CDS             complement(1726..>3340)
                     /locus_tag="VF097_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX6261749.1"
                     /translation="GALDAEAVAAVREEAMPEVRDADELHDLLLSLGVLPLEAGWVEM
                     AEALRAAGRATVAHWDGGSAWVAAERLARVRAAIPGLRLEDELPSLGDEAPPREEAVR
                     DLVHGWMLVAGPVTAGALSGRIGLPQGDVDRALLSLEASGIVLRGRFTGAPELEWCER
                     RLLSRIHRRTIRRLRAEIEPVDPAALMRFLLRWQHVQPGTQLHGREGMLQVLNQLQGF
                     ELPAPAWEAHVLPARVKGSRGSDLEELCLSGTVTWGRIAPPDEEAPRRGRAGRGTPLT
                     FVLREDLHSLRPPGPGLDEILPRLSTRAADVTAHLRDRGASFLPDIVRATGRLPAEVE
                     EALWELVAHGIVSGDGVAGLRSLLAGSAKRPARRLRAIPGGRPRHLPAGRWALFGDGE
                     PGPEEERLAALAGQLLRRYGVVFRELLARERGLPPWRDLVRLYRRWEARGEIRGGRFV
                     AGFVGEQFALPEAVESLRGARRAEPDREPLLLSAADPLNLVGILLPGERISPRSGSWI
                     ALQDGAAVDTGRLGEVRSRLRTAQAQGTS"
BASE COUNT          430 a         1207 c         1242 g          461 t
ORIGIN      
        1 gtggactgca tctacgaggg cggccgcatg ctctacatcc agcccgacga gtgcgtggac
       61 tgcgacgcgt gcgagccggt ctgcccggtg acggccatct tcgccgagga cgacgttccc
      121 gaagagtgga agaacttcac cccggtgaac gcggagttct tcaccgagga gatcgccggg
      181 tacacctcgc cgggtggggc cgcgcaggtc gggcccgtgg acgcggatca cccggtcgtg
      241 gccgggtggg aggcgccggc ctaagcgtca gtccgaggcg tcggtctctg cgagctggat
      301 ggtgcagctc gccatcccgc gcttctccag gtaccgctgg tggtagtcct cggccagcca
      361 gaaggcctgg gccgggacga tctccgtcgc gatcggcttg gagaacctgg cctgggcctt
      421 ggccagcgag cgcttcgcga tcagcgcctg gtcctcgtcg tgggtgaaga tcgccgagcg
      481 gtactgcgtc ccgacgtcgg gtccctggcg attcacctgg gtggggtcgt ggatcgtcca
      541 gaacagctcc acgaggtcct cgtaggagac gcgctcggga tcgaactcga cctgggtgac
      601 ctcggcgtgc ccggtgcggt cggtgcagac gtccttgtag ctggggttgt cgagggagcc
      661 gcccatgtaa ccgacctcgg tgctcacgac cccgtccagc ttccggtagt ccgcctcgac
      721 ctgccagaag cagcccgccg cgaacgtcgc cttctgcgtc tcgcccatgc agtcctcctc
      781 ccgtgggtct tccatgctaa ccggaggggg gccgctctgt gttcccgggc ccggaacgac
      841 ggaaccgccg aggcccgaag acctcagcgg ttccagggac gtttgtactc cgggaaggct
      901 ggccggcgca acccctcccg ctcgttttgt cactcttctt cggctcgtgg atgatgtgcg
      961 cgtgagctcc ggatccctct ccttcgatcg cgccgcgtcg tactacgacc ggacccggcc
     1021 gatctcgcgg acggccctgc ctcgtgtgat ggagctgctc gtcagggagc tcgagggtcg
     1081 gggccactgc ctcgacatgg gcgtgggcac cggcatcgtg tcgatcccgc tggcggagcg
     1141 ggggatcccc gtgatcgggc tggatctctc gcgtcccatg ctagagcggc tccgggagaa
     1201 ggcgggcggc gcggcgcccc tccccctggt ccaggcggat gcgaccgcga tgccgttccg
     1261 cgacggggcc ttcgggggcg ccgtgatccg tcacgtcctc catctgatcc cggattggga
     1321 gcgggcgctg ggggagctcc tgcgggtcgt gcggcctgga ggcgtcgtgc tcgtgaacca
     1381 cggggagtac ccggaggggc tgcgcgagct cttcgatcgc ttcgtccggg aggcgagggt
     1441 ggagcgtccg tgggtggggc tgggccacga cgagctcgac cgggtccacg gctggatgcg
     1501 gtcgaggggc gccgctatcc ggcccctcga gccggtccgt gaggaggtgg aggagccgct
     1561 ggagcgcttc gtggagggga tgcgcgaggg ccactactcc tggacctggg aagtggagga
     1621 tcgggtgcgc ctcgaggccg cgaatcgcgt ggctgcttgg gccgcccggg agctggggcc
     1681 cctcgaccgt ccgatgagac gggaggccga gatcgccttc tgggcctacg acgtgccctg
     1741 agcctgggcg gtgcggagcc ggctgcggac ctcgcccagg cgaccggtgt cgaccgcggc
     1801 gccgtcctga agcgcgatcc acgaccccga gcgggggctg atgcgctccc cgggaaggag
     1861 gatcccgacc aggttgagcg ggtcggccgc cgagagcagg aggggctcgc ggtcgggctc
     1921 ggcccggcgg gccccccgga gcgattcgac ggcctcgggc agcgcgaact gctccccgac
     1981 gaagccggcc acgaagcgcc cgccccggat ctcgccgcgg gcctcccagc ggcggtagag
     2041 gcgcacgagg tcgcgccacg gggggaggcc gcgctcgcgg gccagcagct cgcggaacac
     2101 gaccccgtag cggcggagca gctggcccgc gagcgcggcc aagcgctcct cctcgggccc
     2161 gggctccccg tccccgaaca gcgcccaccg ccccgcgggg aggtgccgag ggcggccacc
     2221 ggggatggcc cggaggcgcc gggccggccg cttggcagag cccgccagca ggctgcggag
     2281 gccggccacg ccgtccccac tcacgatccc gtgggccacg agctcccaga gggcctcctc
     2341 cacctcggcc gggaggcgtc cggtggcccg gacgatgtcc ggcaggaagg acgcgccgcg
     2401 gtcgcgcagg tgcgcggtca cgtcggcggc cctcgtcgac aggcgcggca ggatctcgtc
     2461 caggcccggc cctggggggc ggagcgagtg gaggtcctcg cggagcacga aggtcagggg
     2521 ggtgccgcgc cctgcgcgac cgcggcgcgg cgcctcctcg tcgggcgggg cgatcctgcc
     2581 ccaggtgacc gtccccgaga ggcacagctc ctcgaggtcc gatccgcgcg accccttcac
     2641 ccgggccggc agcacgtgcg cctcccaggc gggcgcgggg agctcgaagc cctggagctg
     2701 gttcagcacc tgcagcatcc cctcccgacc atgcagctgg gtcccgggct ggacgtgctg
     2761 ccaccggagg aggaaccgca tcagcgccgc ggggtccacc ggctcgatct cggctcgcag
     2821 gcgccggatc gtccggcggt ggatccgtga gagcaggcgc cgctcgcacc actcgagctc
     2881 gggcgccccg gtgaagcggc cccgcaggac gatccccgag gcctcgaggc tcagaagggc
     2941 ccgatccacg tcgccctgag ggaggccgat ccgcccggac agcgctccgg ccgtcaccgg
     3001 gcccgccacc agcatccatc cgtggacgag gtcccggacc gcctcctcgc gcggcggcgc
     3061 ctcgtcgccc agcgagggca gctcgtcctc caggcggaga cccggtatcg cggcccggac
     3121 cctcgccagc cgctccgcag cgacccaggc gctgcccccg tcccaatgag ccacggtcgc
     3181 ccgtccggcg gcgcgcagtg cctcggccat ctcgacccac cccgcctcca gcgggagcac
     3241 ccccagcgac aggaggaggt cgtgcagctc gtcggcgtcg cgcacctcgg gcatcgcctc
     3301 ctcgcgcacg gccgccaccg cctcggcgtc cagcgcgccg
//
LOCUS       DASUTZ010000059         5588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_922938, whole genome shotgun sequence.
ACCESSION   DASUTZ010000059 DASUTZ010000000
VERSION     DASUTZ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922938"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /gene="nadA"
                     /locus_tag="VF097_02760"
     CDS             complement(<1..327)
                     /gene="nadA"
                     /locus_tag="VF097_02760"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX6261750.1"
                     /translation="MSIVEPDLRDPALESFEAFQGAVRSLAEERDAVILAHNYELPWI
                     QDVADVVGDSLELSRKAAETGASTIVFCGVHFMAETAKILSPDRTVLIPDLRAGCSLA
                     ESITADQ"
     gene            complement(350..1564)
                     /locus_tag="VF097_02765"
     CDS             complement(350..1564)
                     /locus_tag="VF097_02765"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6261751.1"
                     /translation="MSDPVVIVGGGLAAGAAVQTLREEGEDGPIVLIGAERHLPYERP
                     PLSKQYLRGEADAAALPLHEDASYEEWGIDARIGVRATGLDVADRTVELEGGSTVGYR
                     VLLLATGGRARILGAEPSDRVCYLRTVEDADRLRRHLGGGKHLLVVGAGFIGSEVAAS
                     ARAIGTDVTMIERAASPLNRVLGEGMGRVLAEIHRERGVDLRLETGLTEVRETGESVI
                     VETDEGDALEGDAVVIGAGIIPNTELAEAAGLEIDNGIAVDARCAASAEGVFAAGDVA
                     AHDHPVFGRIRVEHFDNALKMGAHAAKAMLGSPEPFDDAHWFWSDQYDVNLQMGGFAA
                     TWDEIVVRGSIEDRDFTAFYMKDGMVRSVLSIGRPRDVRRGMKVISAQVHPDVEALRD
                     DDVDVGSLLPGA"
     gene            complement(1561..1869)
                     /locus_tag="VF097_02770"
     CDS             complement(1561..1869)
                     /locus_tag="VF097_02770"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEX6261752.1"
                     /translation="MEETRIAGGADIPEGEVRAFEVGDAEIAVANVGSELLAFSNVCT
                     HQQCSLDDGDLEDASIICACHGSQFDLRTGEVISPPATEPIEVYGVAVEGDDLVISGS
                     "
     gene            1953..3485
                     /gene="hutH"
                     /locus_tag="VF097_02775"
     CDS             1953..3485
                     /gene="hutH"
                     /locus_tag="VF097_02775"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEX6261753.1"
                     /translation="MEPVLLGPDPLATADVVAVARGDRAVAIDPAALDDVAAARSRIE
                     EHLGSGQAIYGITTGFGALAGVRVEEEDLARLQRNLIRSHAAGAGEPLPREIVRAMLL
                     LRARALASGRSGVRPEVVERYVALLNEGITPAVPDRGSVGASGDLAQLAHIALAAMGE
                     GEVLAEAGTEPSRLALARAGIEPLSLQAKEGLALINGTEGMLAVGVLAHHDAAQLLRL
                     ADVTAAMTIESALGTDRPFAEDLMALRPHPGQAASAENLRRLLARSEIVAGHRESEHA
                     VQDPYSMRCAPQVHGATRDAHGFAGEVFARELGSVVDNPVILPDGRVESNGNFHGEPL
                     AQALDFLGIALTGLASIAERRTAWILGIGAERGLPPFLSREPGLGSGYMLAQYTQAAL
                     VSECKGLAHPSSVDSIPTSGLQEDHVSMGWHGALRVREIVGRLRTVLAAEAMCGTQAL
                     DLRRPARPGEGTEAARAAIRERVPFLEHDREVAADLRAVEEMLQDGSLLGAVEAATGE
                     LR"
     gene            3482..5137
                     /gene="hutU"
                     /locus_tag="VF097_02780"
     CDS             3482..5137
                     /gene="hutU"
                     /locus_tag="VF097_02780"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005168430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEX6261754.1"
                     /translation="MTREVRAPRGTELSCKGWPQEAALRMLMNNLDPEVAERPEDLIV
                     YGGTGRAARSWDAFDAIVRSLRELEDDETLLVQSGKPVGVFRTHPWAPRVLISNAMLV
                     PKWADWETFRRLEREGLTMYGQMTAGSWIYIGTQGILQGTYETLAELAAQRFQGTLGG
                     RIVLTAGLGGMGGAQPLAVTMNEGVALVVEVDPARIKRRIKTRYLDRETDDLNEAVRW
                     AEEARAEGEAVSIGLLGNAAETHPELLRRGFHPDVVTDQTSAHDPLEGYVPAGLTLEE
                     AAELRRTAPDDYVWRARASMAAHVEAMVGFMDAGAVTFDYGNNLRAEALQGGAERAFE
                     YPGFVPAFVRPMFCEGKGPFRWAALSGDPEDILTTDRVIAELFPEDERLHRWLRMAEE
                     RVAFQGLPARICWLGYGERARAGLRFNELVASGELRAPIVIGRDHLDSGSVASPNRET
                     EGMADGSDAIADWPILNALVNTAAGAHWVSVHHGGGVGIGSSIHAGMVVVADGTDEAA
                     LRLERVLTTDPGTGVVRHADAGYERAIEVARERGIRLPMDEEG"
     gene            5140..>5588
                     /locus_tag="VF097_02785"
     CDS             5140..>5588
                     /locus_tag="VF097_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEX6261755.1"
                     /translation="MSEDPHWPRASEWLSGSHAPHPRRRLAVLGVPLHLGSITPSRAD
                     LAPAAIREALGRYSTWDGETRGDIEIVAAEDHGDLPLADVKPDHALEPVVEAMQGLDA
                     HALVLLGGDNSVTRPGVHGLGVPLDRVGLVTLDAHFDLRDTDGGMRNG"
BASE COUNT          776 a         2045 c         2013 g          754 t
ORIGIN      
        1 ctggtctgcc gtgatcgact cggccagcga gcagccggct cggagatcgg ggatcagcac
       61 cgtccggtcc ggcgagagga tcttcgccgt ctcggccatg aagtggaccc cgcagaagac
      121 gatggtcgag gcgcccgtct ccgcggcctt cctcgacagc tccaacgagt cgcccacgac
      181 gtcggccacg tcctggatcc agggaagctc gtagttgtgg gccaggatca cggcgtcgcg
      241 ctcctcggcc agcgagcgca cggcgccctg gaacgcctcg aacgactcga gggccggatc
      301 ccgcaggtcg ggctcgacga tgctcatcgc atacccatga tacggaggct caggcgcccg
      361 ggaggaggga gccgacgtcc acgtcgtcgt cgcggagcgc ctccacgtcg ggatggacct
      421 gtgccgagat gaccttcata ccccttcgga catctcgggg acgaccgatc gagaggaccg
      481 accgcaccat cccgtccttc atgtagaacg ccgtgaagtc gcggtcctcg atcgagcccc
      541 gcaccacgat ctcgtcccac gtcgcggcga acccgcccat ctgcaggttg acgtcgtact
      601 ggtccgacca gaaccagtgc gcatcgtcga aaggctccgg cgaccccagc atggccttgg
      661 ccgcgtgcgc tcccatcttc agggcgttgt cgaagtgctc cacgcggatc cggccgaaca
      721 cggggtggtc gtgggccgcc acgtcgccgg cggcgaacac cccctcggcc gacgcggcgc
      781 agcgtgcgtc gaccgcgatc ccgttgtcga tctcgagccc cgcggcctcc gcgagctcgg
      841 tgttcgggat gatgccggcg ccgatcacca cggcgtcgcc ctcgagggcg tcgccttcgt
      901 cggtctccac gatcaccgac tcgcccgtct cccgcacctc ggtcagaccg gtctccaggc
      961 ggaggtccac gccccgctcc cggtggatct cggccagcac ccggcccatc ccctcgccga
     1021 gcacccggtt gagcggcgag gcggcccgct cgatcatcgt cacgtcggtg ccgatggccc
     1081 gggccgaggc cgcgacctcg gacccgatga agccggcgcc cacgacgagg aggtgcttcc
     1141 cgccgccaag gtgtcggcgc agccggtcgg cgtcctccac cgtccgtagg tagcagacgc
     1201 ggtcggaggg ctccgccccc aggatccggg cccggccgcc cgtggccagc agcagcacgc
     1261 ggtagccgac cgtggaccca ccctcgagct cgaccgtccg atcggcgacg tccagccccg
     1321 tggcccggac cccgatccgc gcgtcgatcc cccactcctc gtaggaggcg tcctcgtgga
     1381 ggggcagggc ggcggcgtca gcctcccccc tgaggtactg cttggagagc ggcgggcgct
     1441 cgtagggaag gtggcgctcg gcgccgatca gcacgatcgg gccgtcctcg ccctcctcgc
     1501 gcagcgtctg caccgcggcc cccgccgcca gcccgccgcc gacgatgacg acaggatcgc
     1561 tcacgatccg gagatgacga ggtcgtcgcc ctcgacggcg accccgtaaa cctcgatcgg
     1621 ctccgtggcg ggagggctga tcacctcgcc ggtccgcagg tcgaactggc tcccgtgaca
     1681 ggcgcagatg atcgacgcgt cctccagatc cccgtcgtcg agggagcact gctgatgcgt
     1741 gcagacgttg ctgaaggcga gcagctcgct ccccacgttg gctacggcga tctccgcatc
     1801 gcccacctcg aacgcgcgga cctccccctc ggggatgtcc gcgccgccgg cgatcctcgt
     1861 ctcctccatg cgaccccctt ggtcgtgcga ccgtcccatc ctagggcacg gaccggattg
     1921 cggtccgcgg gccgggggcg tgaaatgtgg ccgtggaacc cgttctgctc ggccccgatc
     1981 cactcgccac ggccgacgtc gtcgccgtgg ctcgcgggga tcgagccgtc gcgatcgacc
     2041 ccgctgcgct cgatgatgtg gccgcggccc ggtcccggat cgaggagcac ctgggcagcg
     2101 ggcaggccat ctacggcatc accaccgggt tcggtgccct ggccggcgtg cgggtggagg
     2161 aggaggatct cgcccggctc cagcggaacc tgatccggag ccacgcggcc ggtgccggcg
     2221 agcccctccc ccgggagatc gtgcgcgcca tgctcctcct gcgggcgagg gctttggctt
     2281 cgggccgctc cggcgtccgt cccgaggtcg tcgagcggta cgtcgccctc ctcaacgagg
     2341 ggatcacccc ggccgtgccc gaccggggct ccgtgggcgc cagcggcgac ctggcccagc
     2401 tggcccacat cgctcttgcc gcgatgggtg agggtgaggt gctggccgag gccggcaccg
     2461 agccctcccg cctggcgctg gcccgcgcgg ggatcgagcc gctctcactc caggccaagg
     2521 agggtctggc gctgatcaac ggcaccgagg ggatgctcgc ggtgggcgtg ctcgcgcacc
     2581 acgacgcggc gcagctgctg cgcctggccg acgtcaccgc ggcgatgacg atcgagagcg
     2641 cgctcgggac cgaccgcccc ttcgccgagg acctgatggc gctccggccc catccggggc
     2701 aggccgcctc cgcggagaac ctccggcggc tcctggcgag gagcgagatc gtggccggcc
     2761 accgcgagag cgagcacgcc gtccaggacc cctactcgat gcggtgtgcc ccccaggtcc
     2821 acggcgcgac gcgggacgcc cacggcttcg ccggcgaggt cttcgctcgg gagctcggct
     2881 cggtggtcga caaccccgtg atcctccccg acgggcgggt cgagtcgaac ggcaacttcc
     2941 acggcgagcc tctggcccag gccctcgact tcctcgggat cgccctgacc ggcctggcca
     3001 gcatcgccga gcgccggacc gcctggatcc tcggcatcgg cgctgagcgc ggcctccccc
     3061 cgttcctctc gagggagccc ggcctcggct caggctacat gctcgcccag tacacgcagg
     3121 cggcccttgt gtccgagtgc aagggcctgg cccatccgtc gagcgtcgac tcgatcccca
     3181 cctccgggct ccaggaggac cacgtgagca tgggatggca cggcgccctg cgggtccgcg
     3241 agatcgttgg gcgcctgagg accgtgctcg cggccgaggc gatgtgcggc acgcaggcgc
     3301 tcgatctccg tcgcccggcc cgcccgggag agggcaccga ggcggcccgg gcggcgatcc
     3361 gggagcgcgt cccgttcctg gagcatgacc gggaggtggc cgcggacctc cgcgcggtcg
     3421 aggagatgct ccaggacggg tccctgctgg gcgccgtgga agcggccacg ggggagctgc
     3481 gatgacgcgc gaggtgcggg cgccgcgagg gaccgagctc tcgtgcaagg gctggccgca
     3541 ggaggccgcc ctccggatgc tgatgaacaa cctcgatccc gaggtggccg agcggcccga
     3601 agacctgatc gtctacggag gcacgggccg agccgcccgc tcatgggacg ccttcgacgc
     3661 gatcgttcgg tcgctgcggg agctcgagga cgacgagacc ctcctggtcc agtccgggaa
     3721 gccggtcggc gtcttccgga cgcacccgtg ggcgccccgg gtgctgatct cgaacgccat
     3781 gctggtgccg aagtgggccg actgggagac cttccggcgc ctggagcgcg agggcctgac
     3841 gatgtacggg cagatgacgg ccggctcgtg gatctacatc gggacccagg ggatcctcca
     3901 gggcacctac gagacgctgg cggagctggc cgcccagcgc ttccagggga ccctgggcgg
     3961 ccggatcgtc ctgaccgcgg gcctcggggg catgggcggc gcccagccgc tcgcggtgac
     4021 gatgaacgag ggcgtggcgc tggtggtcga ggtcgacccc gcgcggatca agcgccgcat
     4081 caagacgcgc tacctcgacc gcgagaccga cgacctcaac gaggcagtgc gctgggccga
     4141 ggaagcccgt gccgagggcg aggccgtgag catcgggctc ctcgggaacg cggccgagac
     4201 ccaccccgag ctcctccgcc gaggcttcca ccccgacgtc gtgaccgatc agacctccgc
     4261 ccacgacccg ctcgagggct acgtccccgc gggactgacg ctcgaggagg cggccgagct
     4321 ccgccggacg gcccccgacg actacgtgtg gcgggcccgc gcctcgatgg ccgcgcacgt
     4381 cgaggcgatg gtcggattca tggacgccgg cgcggtcacg ttcgactacg ggaacaacct
     4441 cagagccgag gcactgcagg gcggcgcgga gcgggccttc gagtacccgg ggttcgtccc
     4501 ggcgttcgtc cggccgatgt tctgcgaggg caaggggccg ttccgctggg cggccctctc
     4561 cggtgatccg gaggacatcc tcacgaccga ccgcgtgatc gcggagctct tccccgagga
     4621 cgagcggctc caccggtggc tccggatggc cgaggagcgg gtggccttcc aggggctgcc
     4681 cgccaggatc tgctggctcg gctacggcga gcgggcccgg gccggcctcc gcttcaacga
     4741 gctcgtggct tccggggagc tgagggcccc gatcgtgatc gggcgcgacc acctcgactc
     4801 gggcagcgtg gcctcgccga accgcgagac ggaggggatg gccgacggct ccgacgcgat
     4861 cgccgactgg ccgatcctca acgccctcgt caacacggcc gccggcgcgc actgggtgag
     4921 cgtccaccac ggcggcggcg tggggatcgg gtcgtcgatc cacgcgggga tggtcgtggt
     4981 ggccgacggc accgacgagg cggcgctgcg gctggagcgg gtgctcacca cggatccggg
     5041 gacgggcgtg gtccggcacg cggacgcagg ctacgagcgg gcgatcgagg tggcccggga
     5101 gcgcggcatc aggctcccga tggacgagga gggctgagca tgagcgagga tccgcactgg
     5161 cctcgagcga gcgagtggct gtccggctcc cacgcgccgc atccacggcg gaggctggcc
     5221 gtcctcggcg tcccgctgca cctcggctcg atcacgccca gccgtgccga cctggccccc
     5281 gccgcgatcc gcgaggcgct cggccggtac tccacctggg atggcgagac gaggggcgac
     5341 atcgagatcg tcgcggccga ggatcacggc gacctcccgc tggcggacgt gaagccggac
     5401 cacgccctcg agccggtggt cgaggcgatg cagggcctcg acgcgcacgc cctcgtcctg
     5461 ctcgggggcg acaacagcgt gacgaggccc ggggtccacg ggctcggcgt cccgctcgac
     5521 cgggtgggtc tggtgacgct cgacgcccac ttcgacctca gggacacgga cgggggcatg
     5581 cgcaacgg
//
LOCUS       DASUTZ010000060         2960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1311445, whole genome shotgun sequence.
ACCESSION   DASUTZ010000060 DASUTZ010000000
VERSION     DASUTZ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311445"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VF097_02790"
     CDS             <1..193
                     /locus_tag="VF097_02790"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX6261756.1"
                     /translation="RQVVTEALASLSPRQRAALVLTEGLGYSAEDAGRMLGVKGSTVR
                     ALNFQARAALRRSQEDGDE"
     gene            186..986
                     /locus_tag="VF097_02795"
     CDS             186..986
                     /locus_tag="VF097_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261757.1"
                     /translation="MSRYGALVEREMARIAPPSFSLEDLSRLRARRQRRQRVAARAVA
                     LLVAAVAVGLGIALTRVLSPGEGQPAGPRPQTRVFAPADLETVLLGPDETPAGAPHRR
                     TATGRSAIEAPLKNASKLSIPDLMGPGTGFVGARTVEFLRDFQGVPDSLFFASVGVVY
                     DDAESAGRALLLLVEDFQGRAADASREPVASLGMQAFVVRGTSMIKPPSSTDVVYIWR
                     IENLVLLAAGGDSVGRGAPEEARLVAHEMHARAMEAGAGRPGALGGAG"
     gene            1118..2407
                     /locus_tag="VF097_02800"
     CDS             1118..2407
                     /locus_tag="VF097_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX6261758.1"
                     /translation="MSTDWPAYGLDHANSMHNLAEDTLSPENVAELREAWRLEGIGGV
                     TGTPLVLDGVVYLADWHGAVHAVDAGDGEVLWQEQVADVPISGTVAITGEVVVVGDLG
                     GVLHARDRGSGEKVWSTTLNPAGASLFASPVVIEDTVVIGKTDTELERDDPDFRASIV
                     AVDLEDGSERWRLHTDPGDRHGYWVPVWSSVAYDAERRLIYVGTGNTNQPEAGGAGGP
                     GERAPIDLPLADGVLAIEQQTGEMAWFFKLIEEDAQRDFDVGASPNLFTVGGHDVVGA
                     GGKSGDYVLLDRDTGEMIWKTHLSEGSAGGGVMSTAAVGDGVVYVGSNDAGAEGTIFA
                     LAAEDGSIIWERALDEPIIGGSMAMANGVLYRGTWGGSVLALDAEDGTVLWAEDLDAP
                     LAGGFSIVNGTLYVGYGAGAPPVLGPVDGGLVAYRLP"
     gene            complement(2588..>2960)
                     /locus_tag="VF097_02805"
     CDS             complement(2588..>2960)
                     /locus_tag="VF097_02805"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX6261759.1"
                     /translation="LQAGGLALWMLGVAVLLWASWVMKRYLAVDGLAEEHELVTHGPY
                     RYLRHPVYVSFAAIAIGTALVFRSFIVLGLSVVLMATGRWWADAEERLLASPEAFGDA
                     YRTYAVRTGRFLPRLRRPLGE"
BASE COUNT          456 a          980 c         1074 g          450 t
ORIGIN      
        1 tcgtcaggtc gtgaccgaag cgctcgcatc gctgagcccc cgccagcggg ccgccctcgt
       61 cctcaccgag gggctcggtt acagcgccga ggacgcgggg cggatgctgg gggtgaaggg
      121 ctcgacggtt cgggcgctga acttccaagc gcgggcggcg ctcaggagga gccaggagga
      181 cggggatgag taggtacggg gctctcgtcg agcgcgagat ggcgcgcatc gccccaccgt
      241 ccttctccct cgaggatctc agccgactgc gagcgcggag gcagcggagg cagcgcgtcg
      301 ccgcgcgggc ggttgcgctc ctcgtcgccg cggtggctgt cgggctcgga atcgcgctga
      361 cgcgggtgct ctcccccggc gaaggacagc ccgccggtcc ccgtccccag acgcgagtgt
      421 tcgcgcctgc ggatctggag acggtcctcc tgggtcccga tgagaccccc gcgggcgccc
      481 cgcacaggcg gacggcgacc ggtcgctcgg ccatcgaggc gccgctcaag aatgcctcga
      541 agctctcgat cccggatctg atgggaccgg gcacggggtt cgtgggcgcc cgaaccgtgg
      601 agttcctgcg cgacttccag ggcgtccccg actcgctgtt cttcgcctcc gtgggcgtgg
      661 tctacgacga tgccgagagc gctggacgag ccctcctgct cctcgtcgag gacttccagg
      721 ggcgcgcggc ggacgccagc cgggagcccg tcgccagcct ggggatgcag gcgttcgtcg
      781 tgcgggggac gtcgatgatc aagcccccct cgtcgacgga cgtcgtctac atctggcgga
      841 tcgagaacct ggtcctcctg gcagcggggg gcgactccgt cggccgcggc gctcccgagg
      901 aggctcgttt ggtggctcac gagatgcacg cgcgagcgat ggaggccggt gcggggaggc
      961 ccggtgcact tgggggtgcg ggatgaaccg tagccatctc tgccgagtcg ctggcatcct
     1021 caccaccctg gccctggtcc tcggggcctg tgggggggag cggaccgatc gcgccgatgc
     1081 gaccgacccg gccgaggacg ggacgaatag gagcgagatg agcacggact ggcccgccta
     1141 cggactggat cacgccaact cgatgcacaa ccttgccgag gacaccctct cgcccgagaa
     1201 cgtcgcagag ctgagggaag cctggcggct ggagggcatc ggtggcgtga ccggcacgcc
     1261 gctggtcctg gatggtgtcg tctacctcgc cgactggcac ggggcagtcc acgcggtcga
     1321 tgccggcgac ggcgaggtgc tgtggcagga acaggtagcg gacgtgccga tcagcggcac
     1381 ggtggcgatc acgggtgagg tcgtcgtcgt gggtgacctc ggtggtgtgc tccatgctcg
     1441 cgaccgggga tccggtgaga aggtctggtc gacgaccctg aatccggccg gagcgagcct
     1501 gttcgcctcc cccgtcgtga tcgaggacac ggtcgtgatc gggaagacgg acaccgaact
     1561 ggagcgggac gaccctgact tccgcgcgag catcgtggcg gtggatctcg aggacgggag
     1621 tgagcggtgg cgcctgcaca ccgacccggg agatcggcac ggctactggg tgccggtgtg
     1681 gtcctcggtc gcctacgacg ccgagcgcag gctgatctac gtcggcacgg ggaataccaa
     1741 ccagccggaa gctggaggag cggggggtcc cggcgagcga gccccgatcg accttcccct
     1801 cgccgacggc gtcctcgcca tcgagcagca gacgggtgag atggcctggt tcttcaagct
     1861 catcgaggaa gacgcgcaga gggacttcga cgtgggcgcc tcccctaacc tcttcacggt
     1921 cggcggccac gacgtggtcg gggcaggggg caaatctggg gactacgtcc tcctcgaccg
     1981 cgacaccggc gagatgatct ggaagacgca cctcagcgaa ggaagcgccg gagggggagt
     2041 gatgtccacc gcggccgttg gggacggcgt tgtctatgtg ggctcgaatg acgccggcgc
     2101 tgaagggacc atcttcgccc tggccgcgga ggacgggtcc atcatctggg agcgagcgct
     2161 cgatgaaccc atcatcggag gaagcatggc catggccaac ggcgtcctct accgcggcac
     2221 ctggggaggc tcggtcctgg cccttgacgc agaggacggc acggtcctct gggccgagga
     2281 tcttgatgcc cccctcgcgg gagggttctc catcgtcaac ggcactctct acgtcggcta
     2341 cggggccgga gcccctcccg tcctgggacc ggtggacggc ggcctggtcg cctacaggtt
     2401 gccctagcac cacagggtcc ggtcgggcgg actcattcgg ctatctcgtt cccctccttg
     2461 ggcatacgtg gaggaagctc gcccgggggg ccctcgcgcg tcttcggcaa agctctccgt
     2521 ggatgggtcg cagccgtgct ccgatctccc agatctgacc cgctacctgc gtcaccggct
     2581 tcggccatca ctcccccagg gggcgtctga gcctcgggag gaatcggccg gttcggacgg
     2641 cgtaggtccg gtatgcgtcg ccgaacgcct caggggaggc cagcaatcgt tcttcggcat
     2701 ccgcccacca ccggcccgtg gccatcagca ccaccgacag ccccagcacg atgaagctgc
     2761 ggaagaccag ggcggtcccg atcgcgatcg ctgcgaacga cacgtagacc gggtggcgaa
     2821 ggtaccggta ggggccatgg gttaccagct cgtgctcctc cgccagcccg tcgacggcca
     2881 ggtatcgctt catcacccac gaagcccaga gcaggaccgc gacgcccagc atccagagag
     2941 ccaggccgcc tgcctgcagt
//
LOCUS       DASUTZ010000061        11295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1441634, whole genome shotgun sequence.
ACCESSION   DASUTZ010000061 DASUTZ010000000
VERSION     DASUTZ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11295
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441634"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..1006
                     /locus_tag="VF097_02810"
     CDS             89..1006
                     /locus_tag="VF097_02810"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6261760.1"
                     /translation="MQQRERLSAAADAVGGVAAAASAFAFGSAVVLGVVATERGLPIP
                     ALLAIRFTIAAAAVALVLVSLRRSLRAAPGEGLWLLLLGGIGYGAEAGFFYLALQHGT
                     AAAVSLLFFTYPVWVTVFVALMGRGGPGLLVGGSLAAAVAGAALVVASSGGLDIDLTG
                     ILLSFASAVTFALYLILVDWRLRRTPSLVASMWVTAGAAVALALYALVSGEGRMPRDR
                     TEWWAVVGQGVVTSAAFLGLFIGLKRLGPVRVSIVASLEPMATAILAFLVLGQAVRPL
                     TAVGGALIMGAAVTAALARGREKLEQTPP"
     gene            complement(1010..2902)
                     /locus_tag="VF097_02815"
     CDS             complement(1010..2902)
                     /locus_tag="VF097_02815"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HEX6261761.1"
                     /translation="MRRLGPLLAALALSLSPIAPAAGQGAEVSAIDDVFRPETIRIPV
                     GGTVTWINEGRNPHTAAADDEAWDSGNLDPGDTFSRTFDEQGAIPYYCRYHGAAGGLG
                     MAGTILVGDAPEPEDEAGPPQPPPGEGETLDVPADHPTIQAAVDAAEPGDLVLVSPGI
                     YPESVRVTTPFLTIRGTDRNEVVVDGEFSRENGIHVLEADGVTVENMTARNHTLNGFY
                     WTGVEGYRGSYLTAYNNGDYGIYAFDSVYGQFDHSYASGSPDAGFYIGQCRPCHAVIS
                     DVVAEGNALGYSGTNAGGDLYIVRSEWRKNMSGIVPNTLDSQLLAPQGEVVIAGNWIH
                     DNNNRGAPAKRLQYPTFGSGVLLAGGTDNIVVRNRVEGHERYGIAAVPNLDANLWVTE
                     GNEVRANLVRGSGEADLALGGPAAGGDCFAENDHGTSLPPAIEALAGCGLRLTGGAGD
                     LGVTFSLLGRFAQALSGEFPRGDYRTATVPPPQQTMPGAETSPPAPAVDVPMEISLDE
                     VGAPQGAGERIDHQEVTVLGIGVAAPTWWGLLISVYGYLLPLVLYAAWVSVAAWDLVR
                     RDGPTTRARVGWLAVVLLVPFLGPILYLAFGRSPIPAALRWMLVAGGLGAYLIFAGVS
                     FLLASS"
     gene            2991..3380
                     /locus_tag="VF097_02820"
     CDS             2991..3380
                     /locus_tag="VF097_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6261762.1"
                     /translation="MDVERVKSIPLFADLSQSERELVARHADEVEVEAGRHLIEEGEI
                     GWEFFVIESGAVEVKVGDDPVATLGPGDFFGEVALKDESHRRTATVVATEPSTLIVMT
                     GQAFRTFEGDMPALAERIREAAERYGR"
     gene            complement(3382..5340)
                     /locus_tag="VF097_02825"
     CDS             complement(3382..5340)
                     /locus_tag="VF097_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6261763.1"
                     /translation="MDTERSVEHLLIEGREAAARHAWREARELLEAADAVEPLTASDA
                     EVLARSAWWLGDLAACIAFRERAHRTYRKAGQILGAARVALDLSHDYLMKGDGAMGNG
                     WRRRAERLLADQAPAPEHGYLERMRAGAAMAAGDLDGALEHAIRCQELGERFADPDLQ
                     ALGLHEQGRILIRLGRAPEGLAVLDEAIVAAVSGELDPVVTGIIYCNAIEICRDLTDY
                     ARAGEWNDAAKRWCERMAVSGFPGICRVRRAEIGKLRGAWPEAEREARQAVSELKEWY
                     VDVAAAGLYEIGEIRLRMGDFAAAEEAFRDANGLGHDPQPGLALLRLREGEVGAAWAA
                     IQRSLAATSDADRLRRARLLPAAVEIALENGDVGAASGAAAELEAIAETFLSTALRAA
                     ATVARGRILLAEGKAREAGEALREGRGLWAEVDAPYEVAEARRLLGLAYREAGDEDGA
                     RLELEAALTAFETMGGVPDAARVAEELGVAERRAAPGRAVRTLMFTDIVGSTGLIEAI
                     GDDAWSDLQRWHDETLRAAFAQHGGEEVDHAGDGFFVAFHDPASAIECAVAIQRLLAQ
                     HRRTSGFAPRVRIGIHTTEASTKPGGYGGRGVHEAARIGSLGGADEIVVSRGSLPEHT
                     RFPVSEPRSAELKGIEGPVEVVTVDWRP"
     gene            complement(5398..6390)
                     /gene="speB"
                     /locus_tag="VF097_02830"
     CDS             complement(5398..6390)
                     /gene="speB"
                     /locus_tag="VF097_02830"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEX6261764.1"
                     /translation="MDVTRPNEPAAGSSGATFSRLPLVLDPGELKGADVAILGAPIDE
                     TVSYRPGSRFGPRAIRLADVSGGSPPGFPHMELGIDPVQELAVVDHGDIEAVPGGQER
                     SHAILREAVGRVLQAGCIPVILGGDHSILHPDAGAVADHHGPGTVGVIHFDAHADDAE
                     EIWGVRLSHGTPVRRLVEDGSIPGDRILQIGLRGYWPGPEEFAWAREQGLVWYTQGEI
                     DERGLRDVLDDVVERARAWEHVYLSIDIDVVDPGSAPGTGTPEPGGLTPRELLTAVRH
                     LSVELGFAGMEVVEVSPPYDHADVTALLAHRAVLEALSGLALRKSGREASPERP"
     gene            6450..7244
                     /locus_tag="VF097_02835"
     CDS             6450..7244
                     /locus_tag="VF097_02835"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6261765.1"
                     /translation="MGEWVESGGVRLAVEVLGEGEPVTVVAHGLTQSRLELSLFAPFV
                     PGTKVLFDFRGHGESERPGPGAYRMDDFAADVEAVADAHGATVLIGASLGGGATLRLL
                     RRRPDRFERMVILLPARLERGVGSEDARASLFRVAEHLERYPIEEAAERILAAEEAEG
                     AFDGSAAARELRLQALLRMNREGLPHAIRESLDDPPVTDLAPLRLVQAPVLVIGQEGD
                     PVHESWVARELAEALPGGELMLFPDPFAMVQEIPALVRRITSFIAG"
     gene            complement(7285..7638)
                     /locus_tag="VF097_02840"
     CDS             complement(7285..7638)
                     /locus_tag="VF097_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007382341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX6261766.1"
                     /translation="MQAKPVHPIPEGEPLKAVRMMNDRVLVEPPDGDEERRSRGGLVI
                     PATAAGLDRKGVWGRVVAVGPNARTVEVGDEVLLLPESGIEVEIRGRDYVLVREREIH
                     AVATEREDPGSGLYL"
     gene            7673..8542
                     /locus_tag="VF097_02845"
     CDS             7673..8542
                     /locus_tag="VF097_02845"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HEX6261767.1"
                     /translation="MRAQELVREGERRLKRSPAIDHWPADRERREAAALLAKALGRDE
                     EEPEDLDASVPGRVAARFRRLVRRRAGGEPMGHILGWTEFRGLRFRVRPGAFVPRQSS
                     EWTVEQTVRRLRPRRRPVHVDVATGIGPIALAVASEVPKAQVHGTDLMPEGLRQARSN
                     ARELGIRNASFHVGDLLEPLPGRLRGRVDAVTGHIPYVPLGEVDDLPLEVRGFEPEVT
                     LTDYTDHGMSLLGRAVLEARGWLRSGGWLLMEVSPDRSRAVKALMQRAGYGDVRSTKG
                     WPGISRTVVGRWR"
     gene            8533..9180
                     /locus_tag="VF097_02850"
     CDS             8533..9180
                     /locus_tag="VF097_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX6261768.1"
                     /translation="MALARGLLIDFGGVLTTPLGGALRRWCEREGVDPERLAELLKTS
                     YPVTDPTSIVIQIETGRMSVQEFERRLAEVLSEGLHRPVDPDGLLDRMGESIELEPRM
                     IDAVRTAHAAGVHTALISNSWGLHYYPHDLLGELFDEILISGQVGTRKPEDEIYLLAA
                     RGLGLEPPECVFVDDVEVNLHTAERLGMRTLLHEGPERTIAELEALLGVPLGDAR"
     gene            9197..9535
                     /locus_tag="VF097_02855"
     CDS             9197..9535
                     /locus_tag="VF097_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX6261769.1"
                     /translation="MEPDLTTPKGVAERHPHEVVILDVREPAEWDAGRVEGSIHVPLS
                     RLMAGAMPDLDEDRPIVAVCTVGTRSQVAALLLQARGYEAHNLEGGLERWVAEGLPLT
                     TPAGEPGRVA"
     gene            complement(9539..>11295)
                     /locus_tag="VF097_02860"
     CDS             complement(9539..>11295)
                     /locus_tag="VF097_02860"
                     /inference="COORDINATES: protein motif:HMM:NF013682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX6261770.1"
                     /translation="FVNVKVDREERPDVDAIYMDAVQAMTGQGGWPMTVFLTPEGEPF
                     WAGTYFPKEERHGMPAFTRVLRAIAEAWRERRDEIGEQGRKVVEAIGRRVRPSEEPLT
                     EEVLDEAFAGLRASFDPEHGGFGGAPKFPQPMTLEFLFRMHLRGREGALEIATRTLGG
                     MAAGGIRDVLGGGFHRYSVDAAWLVPHFEKMLYDNAQLTLAYLHAFQLTGEERHRDVA
                     SETLEYLLRDLRHERGGFFSSEDADSEGEEGRFYVWSWNELVSAVGQDEARALGGRPE
                     GNWEGTNVLHGESPPRAVRERLLAIRAERVRPATDDKVLAGWNGLAIRALAEAGAALE
                     EPRYVEAAVLAARFVLEEMRREDGRLLRAWRDGRTSGPAYAHDHALLSAGLLALYEAT
                     LDHRWFEAARVLADDLLRLFHDEEHGGFFETGADAEELVIRPKELLDNAVPAAGSVAA
                     EVLQRLSMLTGDARYERAATSALEQVRGVMGRAATGLGHALSAADLSLSPSREIAIVG
                     DPADERTGALLREARRRYLPNAVLAGGGPDDTAAVAAIPLLQDRELVDGAPAAYVCEG
                     FVCRRPITEPAELAAELG"
BASE COUNT         1469 a         4108 c         4108 g         1610 t
ORIGIN      
        1 atgtgggttc ctaacgaggc cggacggcct ccggcgggca actgtagcac acggccgcgg
       61 gacctcccgc gcccgaggaa gggggagcgt gcagcagcga gagcgactct cggcggcggc
      121 cgatgcggtg ggcggggtcg cggccgccgc gtcggcgttc gccttcggct cggccgtcgt
      181 gctgggcgtg gtggccaccg agcggggcct gccgatcccg gccctcctcg cgatccgctt
      241 cacgatcgcg gcggcggccg tcgccctggt gctggtctcg ctgcgccgct ctctgcgcgc
      301 cgcaccgggg gaggggttgt ggctcctcct gctcggtggg atcgggtacg gggccgaggc
      361 cgggttcttc tacctggccc tccagcacgg gacggccgcc gcggtctccc tcctgttctt
      421 cacgtatccc gtgtgggtga cggtcttcgt ggcgctgatg gggcgaggcg gcccggggct
      481 gctcgtcggc gggtcgctgg ccgcggcggt ggcgggcgcc gcgctcgtgg tggcctcctc
      541 gggcgggctg gacatcgacc tcaccggcat ccttctctcc ttcgcctccg cggtgacctt
      601 cgccctctac ctgatcctcg tggactggcg tctgcggcgg acgccctcgc tcgtggcctc
      661 gatgtgggtg acggccggcg ccgccgtcgc gctggccctg tacgcgctcg tcagcgggga
      721 agggaggatg ccccgggatc ggaccgagtg gtgggcggtc gtcggccagg gggtcgtgac
      781 ctccgcggcg ttcctcgggc tcttcatcgg cctgaagcgg ctgggaccgg ttcgcgtctc
      841 gatcgtcgcg tcgctggagc cgatggcgac ggcgatcctc gcgttcctcg tgctggggca
      901 ggccgtgcgc ccgctcacgg ccgtgggcgg ggccctcatc atgggggccg cggtgaccgc
      961 ggcgctggcc cgcggccggg agaagctgga gcagacgcct ccgtgaacgc taggagctgg
     1021 ccaggaggaa cgagaccccg gcgaagatca ggtacgcgcc gaggcccccg gccacgagca
     1081 tccagcgcag cgccgccggg atcggggacc ggccgaaggc gaggtagagg atcgggccga
     1141 ggaagggcac gagcaggacg acggccagcc agccgacgcg cgctcgggtc gtcgggccgt
     1201 cgcgacgcac gaggtcccac gcggcgacag agacccatgc cgcgtagagg acgaggggca
     1261 gcaggtaccc gtagaccgag atgagcaggc cccaccacgt gggggctgcg acgccgatgc
     1321 cgagcacggt cacctcctgg tggtcgatgc gctcgccggc gccctgcggg gcgccgacct
     1381 cgtccagaga gatctccatc gggacgtcga cggccggggc cggcggggac gtctccgccc
     1441 cgggcatggt ctgctggggt ggaggcacgg tggcggtccg gtagtcgccg cgagggaact
     1501 cgcccgacag cgcctgcgcg aacctcccca gcagcgagaa cgtgaccccg aggtccccgg
     1561 ccccgcccgt cagccgcagc ccgcacccgg cgagcgcctc gatggccggc ggcaggctcg
     1621 tgccgtggtc gttctccgcg aagcagtccc cgcctgccgc cggcccgccc agcgccagat
     1681 cggcctcccc cgagccgcgc acgaggttcg ctcgcacctc gttcccctcc gtgacccaca
     1741 ggttggcgtc gaggttgggc acggcggcga tcccgtaccg ctcgtgcccc tcgacccggt
     1801 tccgtacgac aatgttgtcg gttcccccag ccagaagcac cccgctcccg aacgtcgggt
     1861 actggaggcg cttcgccggc gccccccggt tgttgttgtc gtggatccag ttcccggcga
     1921 tcacgacctc gccctggggc gcgagcagct gggagtccag cgtgttggga acgatccccg
     1981 acatgttctt ccgccactca gaccgcacga tgtacaggtc gccgccggcg ttcgtccccg
     2041 agtagccgag cgcgttgccc tcggccacca cgtcgctgat caccgcgtgg cacggccggc
     2101 actgcccgat gtagaagccc gcgtcggggc tccccgaggc gtaggagtga tcgaactgcc
     2161 cgtacacgga gtcgaacgcg tagatcccgt agtcgccgtt gttgtaggcc gtcaggtaag
     2221 agccccggta gccctcgacc cccgtccagt agaagccgtt cagggtgtgg ttccgcgcgg
     2281 tcatgttctc cacggtgacg ccgtcggcct cgagcacgtg gatcccgttt tcgcgggaga
     2341 actccccgtc gacgaccacc tcgttccggt cggtccccct gatcgtgagg aacggcgtcg
     2401 tgacgcgcac gctctccggg tagatgccgg gcgagaccag cacgagatcg ccgggctccg
     2461 cggcgtccac cgccgcctgg atcgtggggt ggtccgcggg cacgtccagg gtctcgccct
     2521 cgcccggcgg gggttgcggg gggccggcct cgtcctcggg ctcgggggcg tctcccacca
     2581 ggatcgtccc cgccatgccc agaccaccgg ccgcgccgtg gtaccggcag tagtagggga
     2641 tcgctccctg ctcgtcgaac gtccgggaga acgtgtcgcc cggatcgagg ttccccgaat
     2701 cccatgcctc gtcgtcggcc gccgccgtat gcgggttccg gccctcgttg atccaggtca
     2761 ccgtgccgcc cacggggatc cggatcgtct cggggcggaa gacgtcgtcg atcgccgaga
     2821 cctcggctcc ctgccccgcc gccggagcga tggggctcag gctcagcgcg agagccgcga
     2881 ggagcggacc gagccggcgc atcggcggga gcctaccaga ggcccgctcc cgagtggccg
     2941 cccgggtcgg atgctggaaa gataccgagg aaccaggacg aggaggctgc atggacgtcg
     3001 agcgtgtgaa gtcgatcccg ctgttcgccg acctctcgca gagcgagcgc gagctggtcg
     3061 cccgccacgc cgacgaggtg gaggtcgagg cgggcaggca cctcatcgag gagggcgaga
     3121 tcggctggga gttcttcgtg atcgagtcgg gcgcggtcga ggtcaaggtc ggggacgatc
     3181 ccgtggccac cctgggcccg ggggacttct tcggcgaggt ggctttgaag gacgagtccc
     3241 accggcgcac ggcgaccgtc gtcgccaccg agccctccac gctgatcgtg atgacggggc
     3301 aggccttccg caccttcgaa ggagacatgc ccgcgctggc cgagaggatc cgggaggcgg
     3361 ccgagcgcta cggtcggtag ctcagggccg ccagtcgacg gtcacgacct cgacgggtcc
     3421 ctcgatcccc ttgagctcgg ccgatcgcgg ctcgctgacc gggaagcgag tgtgctcggg
     3481 gagagaccct cgactgacca cgatctcgtc cgcccctcca agagagccga tccgcgcggc
     3541 ctcgtgtacg ccccggcccc cgtagcctcc gggcttcgtc gacgcctcgg tcgtgtggat
     3601 gccgatgcgc acccgaggcg cgaagccgct cgtccgccgg tgctgcgcca gcaaacgctg
     3661 gatcgcgacc gcgcactcga tcgcggatgc cgggtcgtgg aacgccacga agaagccgtc
     3721 cccagcgtgg tccacttcct cgcccccgtg ctgcgcgaac gcagcgcgca gggtctcgtc
     3781 gtgccagcgc tgcaggtcgc tccacgcgtc gtccccgatg gcctcgatca ggcccgtcga
     3841 gccgacgatg tcggtgaaca tgagcgtgcg gacggctcgg cccggagccg cccgccgctc
     3901 cgcgacgccg agctcctcgg cgacgcgggc ggcatcggga acgcccccca tcgtctcgaa
     3961 ggctgtgagc gcggcctcca gctcgagccg cgccccgtcc tcgtcgccgg cttcccgata
     4021 ggccagcccc aggagccggc gcgcctccgc cacctcgtac ggcgcatcca cctcggccca
     4081 gaggccacgc ccctcccgga gcgcctcgcc cgcctctcgg gcctttccct cggcgaggag
     4141 gatccggccc cgcgcgaccg tcgccgccgc ccgcaaggcc gtggagagga aggtctcggc
     4201 gatcgcttcg agctccgcgg cggctcctga cgcggctccc acatcaccgt tctctagggc
     4261 gatctcgacg gccgccggca gaagccgggc gcggcggagc cggtcggcat ccgaggtcgc
     4321 ggccagggac ctctggatcg ccgcccaagc cgcgccgacc tctccctcgc gcagccggag
     4381 gagcgccaag ccgggctgcg ggtcgtgccc cagcccgttc gcgtcgcgga aggcctcctc
     4441 ggccgctgcg aagtcgccca tccggaggcg gatctctccg atctcgtaga ggccggccgc
     4501 tgcgacgtcc acgtaccact ccttcagctc ggagaccgcc tgccgagcct cccgctccgc
     4561 ttccggccac gccccgcgca gcttgccgat ctccgctcgt cggacgcgac agatcccggg
     4621 gaagcccgag actgccatcc gctcgcacca ccgcttggct gcatcgttcc actcgcccgc
     4681 ccgcgcgtag tcggtgaggt cgcggcagat ctcgatcgcg ttgcagtaga tgatccccgt
     4741 tacgaccggg tcgagctcac cgctcacggc ggccacgatc gcctcatcga ggaccgcgag
     4801 cccctccggg gcgcgtccca ggcggatcag gatccgtccc tgctcgtgaa ggccgagggc
     4861 ctggaggtcg gggtcggcga accgctcgcc gagctcctgg cagcggatcg cgtgctccag
     4921 ggccccgtcc aggtccccgg cggccatcgc ggcgcccgcc cgcatccgct cgaggtaccc
     4981 gtgctcgggg gcgggggcct gatccgccag cagtcgctct gcccgccggc gccatccgtt
     5041 ccccatcgcg ccgtcgccct tcatcaggta gtcgtggctc aagtcgagag ccacgcgggc
     5101 cgctcccagg atctggcccg ccttccgata ggtcctgtgg gcccgctccc ggaaggcgat
     5161 gcaggcggcg aggtccccga gccaccaggc ggacctcgcg aggacctcgg cgtctgaggc
     5221 cgtgagcggc tccacggcat cggccgcttc cagcagctcc cgggcctcgc gccaggcgtg
     5281 ccgggccgcg gcctcccgac cttcgatgag gagatgctcg accgaccgct ccgtgtccat
     5341 gaagcacatg gtcgcacgct cgggctctgg gcggctcgcc gaccgtgtgc gcgagggtta
     5401 cggacgctcg ggcgaggcct ctcgccccga cttccgcagg gccaggcccg agagcgcctc
     5461 caggacggct cggtgcgcca gcagcgcagt cacgtcggcg tggtcgtagg ggggcgacac
     5521 ctcgacgacc tccatcccag cgaatcctag ctcgacggag aggtggcgga cggccgtcag
     5581 gagctcgcgg ggggtgagcc cgcccggctc gggggtcccg gtccccggcg ccgacccggg
     5641 gtccacgacg tcgatgtcga tcgagaggta cacgtgctcc caggcccggg cccgttccac
     5701 gacgtcgtcg aggacgtctc gtagccctcg ctcatcgatc tccccctgtg tgtaccagac
     5761 gaggccctgc tcccgggccc aggcgaactc ctccgggccc ggccagtagc cgcggaggcc
     5821 gatctggagg atccggtcgc cggggatcga gccatcctcg accagcctcc tgacgggcgt
     5881 cccgtgcgag agccgcacgc cccagatctc ctcggcgtcg tcggcgtgag cgtcgaagtg
     5941 gatcacgccc acggtgccgg gcccgtggtg gtcggccacg gcgccggcgt ccgggtgaag
     6001 gatcgagtgg tcgcccccga ggatcaccgg gatgcagccg gcctggagca cccgccccac
     6061 ggcctcccgg aggatcgcgt gcgaacgctc ctgcccgccg gggaccgcct cgatgtcgcc
     6121 gtggtccacg actgcgagct cctggacggg gtcgatcccc agctccatgt gggggaaccc
     6181 cggcggcgat cccccgctga cgtcggccag ccggatcgcc cgcggcccga agcgcgatcc
     6241 cggccggtag ctgaccgtct cgtcgatcgg cgcgcccagg atggccacgt cggcgccctt
     6301 cagctcgccc ggatcgagga ccagcgggag gcgggagaag gtggccccgc tggatccggc
     6361 ggccggctcg ttcggtctcg tgacgtccac ggctgggcca gcctcgccgg tcgagcccgc
     6421 gcacgcaagc ggctggtatc gtccccgcca tgggggagtg ggtcgagtcg ggaggcgtcc
     6481 gcctcgccgt ggaggtcctg ggcgaggggg agccggtcac ggtcgtggcg cacgggctga
     6541 cgcagtcgag gctcgagctg agcctgttcg cgccgttcgt gccgggcacg aaggtgctct
     6601 tcgacttccg agggcacggc gagagcgagc ggcccgggcc gggcgcctac cggatggacg
     6661 acttcgcggc cgacgtcgag gccgtggccg acgcgcacgg agcgacggtg ctgatcgggg
     6721 cctcgctggg cggaggagcg accctgcggc tcctccgacg gaggcccgac cggttcgagc
     6781 ggatggtgat cctgctgccg gcccggctgg agcggggcgt ggggtccgaa gacgcccgag
     6841 ccagcctgtt ccgggtggcc gagcacctgg agcggtaccc gatcgaggag gcggcagagc
     6901 ggatcctcgc ggcggaggag gcggagggag ccttcgacgg gtcggcggcg gccagggagc
     6961 ttcgactgca ggcgctgctc cggatgaacc gcgaggggct gccgcacgcg atccgcgaat
     7021 cgctcgacga tccgccggtc accgatctgg cccccttgcg gctcgtgcag gccccggtgc
     7081 tggtgatcgg gcaggagggg gatcccgtgc acgagtcctg ggtggcgcga gagctggcgg
     7141 aggcgctgcc gggcggagag ctgatgctgt tccccgaccc gttcgcgatg gtgcaggaga
     7201 tccctgccct ggtgagacgc atcacgtcgt tcatcgccgg ctgaaggtcc cgcgagcctt
     7261 ccacgaggtc tcgtcggagg ggcgctagag gtacaagccc gagccggggt cctcgcgctc
     7321 ggtggccacc gcgtggatct cgcgctctct tacgagcacg tagtcccggc cgcggatctc
     7381 gacctcgatg cccgactccg ggaggagcag cacctcgtcg cccacctcga ccgttcgggc
     7441 gttcggcccg acggccacca cgcgccccca cacgcccttc ctgtcgaggc ccgcggcggt
     7501 ggccgggatc accagtccgc cgcgcgaccg ccgctcctcg tcgccgtcgg gcggctcgac
     7561 caggacgcgg tcgttcatca tccggaccgc cttcagcggc tcgccctcgg ggatggggtg
     7621 gaccggcttc gcctgcatcg cggaccagga tactcggtat cgttcatcgt cgatgcgcgc
     7681 acaggagctg gttcgggagg gcgagcggcg gctgaagcgc tcgccggcga tcgatcactg
     7741 gcccgccgac cgggagcggc gggaggcggc cgcgctgctg gccaaggcct tggggcgcga
     7801 cgaggaggag ccggaggacc tcgacgcctc ggtgccgggg cgggtcgccg cccgcttccg
     7861 gcggctggtc cggcgccggg ccggcggcga gccgatgggg cacatcctcg gatggaccga
     7921 gttccggggg ctgcggttcc gggtccgtcc gggtgccttc gtcccccgtc agtcgagcga
     7981 gtggaccgtg gagcagacgg tccggcggct ccggccccga cggcgacccg tgcacgtgga
     8041 cgtggccacc gggatcggcc cgatcgccct cgcggtggcg agcgaggtcc cgaaggccca
     8101 ggtccacggg acggatctca tgcccgaggg gctgcgtcag gcccggagca atgcccggga
     8161 gctcgggatc cgcaacgcga gcttccacgt cggggatctc ctggagcctc tcccgggacg
     8221 gctcaggggg agggtggacg cggtgacggg ccacatcccg tacgttcctc tgggggaggt
     8281 ggacgacctt ccgctcgagg tccgcgggtt cgagcctgag gtcacgctga ccgactacac
     8341 cgaccacggg atgtcgctgc tggggcgcgc cgtcctcgag gcgcgcggat ggcttcgttc
     8401 cggcggctgg ctcctcatgg aggtgagtcc cgaccgctcc cgcgcggtca aggccttgat
     8461 gcagcgcgcg gggtacgggg atgtgcgaag cacgaagggc tggccgggga tctcccggac
     8521 ggtggtgggc cgatggcgct agcgcggggc ctgctgatcg acttcggcgg ggtgctgacc
     8581 acgccgctcg ggggtgcgct gcggcgatgg tgcgagcgcg agggcgtgga cccggagcgc
     8641 ctggccgagc tcctgaagac gtcgtacccg gtgacggacc caacgtcgat cgtgatccag
     8701 atcgagaccg gcaggatgtc ggtccaggag ttcgagcgcc ggctggccga ggtgctctcc
     8761 gaggggctgc accgaccggt ggacccggac gggctcctcg atcggatggg ggagtcgatc
     8821 gagctcgagc cccggatgat cgacgccgtt cggaccgccc acgcggccgg cgtgcacacc
     8881 gcgctgatct ccaactcgtg ggggctccac tactacccgc acgacctgct cggggagctg
     8941 ttcgacgaga tcctgatctc gggccaggtc ggcacgcgca agcccgagga cgagatctac
     9001 cttctcgcgg cccggggcct cggcctggag ccgccggaat gcgtcttcgt ggacgatgtc
     9061 gaggtcaacc tccacacggc cgagcggctg gggatgcgca cgctgctgca cgaggggccc
     9121 gagcgcacga tcgccgagct cgaggccctg ctgggggtgc cgctcggcga cgcccgctga
     9181 tacgatcgct gtccggatgg aacctgatct gaccacgccg aagggcgtcg ccgagcggca
     9241 cccccacgag gtcgtgatcc tcgacgtccg cgagcccgcg gagtgggacg cggggagggt
     9301 cgagggctcg atccacgtgc cgctgagccg gctgatggct ggggcgatgc ccgacctcga
     9361 cgaggatcgc ccgatcgtgg cggtctgcac ggtgggcacc cgcagccagg tcgcggcgct
     9421 gctgctccag gccaggggct acgaggcgca caacctcgag ggaggcctcg agcggtgggt
     9481 ggccgagggc ctgccgctca ccaccccggc cggcgagccg ggcagggtcg cctgagggtc
     9541 agcccagctc cgcggccagc tccgccggct ccgtgatcgg tcggcgacag acgaatccct
     9601 cgcagacgta ggccgcaggt gccccgtcca cgagctcccg gtcctggagg agagggatgg
     9661 ccgccaccgc ggcggtgtcg tccggccctc cgccggccag gacggcgttc ggcaggtacc
     9721 ggcgtcgcgc ctctcggagc agcgccccgg tccgctcgtc ggccgggtcg cccacgatcg
     9781 cgatctcgcg ggagggcgag agcgagagat ccgccgccga gagggcgtgc ccgaggccgg
     9841 tggccgcccg gcccatcacc ccgcgcacct gctccagggc cgacgtcgcg gctcgctcgt
     9901 agcgcgcgtc gccggtgagc atcgagagcc gctgcagcac ctctgcggcg accgagccgg
     9961 cggcagggac cgcgttgtcg aggagctcct tcggtcggat cacgagctcc tcggcgtcgg
    10021 cccccgtctc gaagaagccc ccatgctcct cgtcgtggaa gaggcggagg aggtcgtcgg
    10081 ccagaacccg ggcggcctcg aaccaccggt ggtcgagcgt ggcctcgtag agtgcgagga
    10141 ggccggccga gagaagcgcg tggtcatgcg cgtacgcggg gccgctcgtc ctcccgtcgc
    10201 gccaggcccg gaggaggcgg ccgtcctcgc ggcgcatctc ctcgaggacg aagcgcgccg
    10261 cgaggacggc cgcctccacg tacctcggct cctcaagcgc ggcgccggcc tcggccaggg
    10321 ctcggatcgc cagcccgttc caccccgcca gcaccttgtc gtctgtcgcc gggcggacgc
    10381 gctcggcccg gatcgccagc agtcgctcgc gcacggccct gggcgggctc tccccgtgga
    10441 ggacgttcgt tccttcccag ttcccctcgg gccggccccc cagcgcccgc gcctcgtcct
    10501 ggcccaccgc gctcacgagc tcgttccacg accagacgta gaaccgcccc tcctccccct
    10561 cggagtccgc gtcttcggac gagaagaagc cgccgcgctc gtgccggaga tcccgaagga
    10621 ggtactcgag cgtctcggac gcgacgtctc ggtgccgctc ctcgcccgtg agctggaacg
    10681 cgtggaggta ggcgagcgtc agctgcgcgt tatcgtagag catcttctcg aagtgtggga
    10741 cgagccacgc cgcgtccacg ctgtaccggt ggaagccacc cccgagcaca tctcggatcc
    10801 cgccggccgc catcccgcct agggtccgcg tggcgatctc caacgcgccc tcgcggccgc
    10861 gtagatgcat ccggaagagg aactcgaggg tcatcggctg ggggaacttg ggtgccccgc
    10921 cgaagccccc gtgctccggg tcgaaggagg cccggaggcc ggcgaacgcc tcatccaaca
    10981 cctcctcggt cagcggctcc tcggaggggc gcacccgccg cccgatcgcc tccacgacct
    11041 tccgcccctg ctccccgatc tcgtccctgc gctcccgcca cgcctcggcg atcgcccgca
    11101 gcactcgagt gaaggcgggc atcccgtgac gctcctcctt ggggaagtag gtcccggccc
    11161 agaacggctc gccctcgggc gtgaggaaga ccgtcatcgg ccacccgccc tgcccggtca
    11221 tggcctgcac ggcgtccatg tagatcgcgt ccacgtcggg ccgctcctcg cggtccacct
    11281 tcacgttgac gaagt
//
LOCUS       DASUTZ010000062         7161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_793081, whole genome shotgun sequence.
ACCESSION   DASUTZ010000062 DASUTZ010000000
VERSION     DASUTZ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7161
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793081"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VF097_02865"
     CDS             <1..678
                     /locus_tag="VF097_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261771.1"
                     /translation="TLSVSEGMLQGTTTGTILKDQSGTTISGVIYPDTANVTLFASAK
                     QIEPGSWFLSVQKSAVAFGTSPAAFTVSTCGPGDPSPANPKCIVAEFHNGAPSGYAAH
                     GSCLGYPGVLEGTCPNAETAADVSLISLISDLGGLYTKTDPLKVSWFCDQSACPRGTG
                     GPTSGNPFTTLFVEVDGSFVASPACPSKGTIGPDQKFCTDYVQSSGLAGGDLLLVILF
                     DGDPRWM"
     gene            complement(644..4510)
                     /locus_tag="VF097_02870"
     CDS             complement(644..4510)
                     /locus_tag="VF097_02870"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6261772.1"
                     /translation="MPGTVTDARERLRPYLPRLLLRWIEESPGEASRQVAGSVAFADI
                     SGFTRLSERLAELGKLGAEEVAEAIDSSFTTLLAVAYEEGGSLIKFGGDALLLLFEDD
                     GHAVRASRAAAGMRRALREMGAIRTPRGRVTLRMSIGVHSGTFHFFLVGESHRELVLA
                     GPGTTEAVAMETAAEAGEIVLSPGAAAALPERLLGEAKGPGSLLRRRPPGSPSGLPSF
                     DVTGPPEALARCLPVAVRKAVLGGSLDREHRLVTVAFLRFEGIDRVVEEEGPKAAATA
                     LAAVVEGVQRAVDRHDVTFLGTDVDRDGGKIILASGAPRTVGNDEERILLAVREIAEA
                     GLPLPIRIGVHGGRVFAGDIGPAYRRTYTVMGDAVNLAARLMARAEPGQVISSRWVLR
                     RSRTIFRAERLPAFRVKGKEAPITACTVGAAEGAKAGSSQEHPFVGREREMAVLLEGL
                     ASARAGAGRAVEVIGDAGIGKSRLVRELRDRAGDAAVYTATCELYESQTPYRPFRDLL
                     QGALGVPHDASEREVETTLRGLVERHAPQLLPWLPLLALPLHVDLPPTPEVEELEERF
                     RRERAEQATAELLGALLSGPTMITIEDAHWMDEASASLLSNLVRTVADRPWLLVITRR
                     EHDSSFAAPEGPATIALRPEPLDAAAAQSLLTAASEESPLRPHEVKALAERAGGNPLF
                     LIELLAAARRVGDVEAIPDSVEALIAARIDRLDPVDRALLRRASVLGLAFEPSLVEEL
                     SADPAGPEAWEQLGEFVERENGTLRFRHALIREAAYEGLPYRVRRSLHASAAESIERA
                     SGDRPEEAAELLSLHFFNAGRYPEAWRYSTVAGDQARAVYANVEAADLYQRALDAARR
                     AGGVAPRRIAEVLEALGEVKSRSGLYEDAARAYREARRRADGGPVRDAQLMLKEAETL
                     HWLGLFPRALGWVTRALHTLEGLPGEETAATVAKLSSLYALCRFRQGRLREAGRWAQR
                     AVAEAEQAGDKAALGEAYRVLDMVEWSSGGEAGDTYGRVALGIFEELGDRSMQANLLN
                     NLGVRAVLEARWDEALELLERAEESFSRIGHSQGVIIAAFNRGDLLVRRGQLEEAERR
                     LHEARRMSRAVGDREGVAFATRELGRAAARAGRHREGLDLLGEAREAFTTLGARNEVL
                     ETDAAIAEARLLAGDWSGALELATTSISHALTIEGEPLLPILHRVRGYALLEGGRPEE
                     ARAALEEAVEMGRAQGADDEVAFALAALAGAARVLGDPEAERFDQEAGELNERLGVAF
                     MPPIRGRGTEPRPRRVTSSADPRRRG"
     gene            4703..5113
                     /locus_tag="VF097_02875"
     CDS             4703..5113
                     /locus_tag="VF097_02875"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03667 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEX6261773.1"
                     /translation="MALDRQLEGIAGKLQDEIVVWLTTVSPEGQPQSVPVWFLWRDGA
                     FLIYSQPDKPKLRNLAANPRVSLHFRGTRNGEDIATLEGEARRAEDHPPADQVPPYVE
                     KYAAEIEKLRWTPQSFAADYSQPILVRPTHVLTW"
     gene            complement(5115..5858)
                     /locus_tag="VF097_02880"
     CDS             complement(5115..5858)
                     /locus_tag="VF097_02880"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6261774.1"
                     /translation="MRALLRTWWIEMKLFAREPLTVVFTLALPIVMLFVLASVFGNTP
                     EADVYRGVGPIDYYVPAYAALVTASLGLIALPTHLAEYRERGILRRFRASTVPLWTVF
                     AAHALVAVVASAVGSALLIAVASASYDIAAPVSPALFVGAFALGALTFAAIGGLLGAT
                     MPTTRAAQGAGVLLWFVMLIIAGAGPPPEVLTDATRTVADATPLRHVILLMQDPWLGF
                     GWNGIQTLIVLAWLVGSALLAGLLLRRRV"
     gene            complement(5858..6775)
                     /locus_tag="VF097_02885"
     CDS             complement(5858..6775)
                     /locus_tag="VF097_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6261775.1"
                     /translation="MSPAEPVVLVDALHKRYGDAVAVEDVSFDVRRGEIFGLLGPNGA
                     GKTTTVECLQGLRAPDRGSLRVLGLDPTRQAAELRRRIGSQLQQSALPDRIKVREALH
                     LFGSGADGAEAERLLVEWGLAQKRGARFQALSGGQRQRLFIALALLNHPEVVFLDEMT
                     TGLDPGARRVAWQLISAIRERGTTVVLVTHFMDEAERLCDRVAIVDRGRVVAMGSPQE
                     LIVRHAGAVRVRFTAELRDASFLEAIDGVTSVASRGSEVAVDGSGAVLARVAAALSTR
                     GIEPEDLRLERSTLEEVFLALVGTSEGMG"
     gene            complement(6772..>7161)
                     /locus_tag="VF097_02890"
     CDS             complement(6772..>7161)
                     /locus_tag="VF097_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016211236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX6261776.1"
                     /translation="MVCVQVMGNDAAVAIAGSQGNFELNVFKPVMIRNFLHSVAILAD
                     AMGAFREFCVDGIRPNRERIAELVDRSLMLVTALTPKIGYDKAAEIAKKAHAEGSTLK
                     EAALSLGYLTGEEYDEAVRPERMVGPE"
BASE COUNT         1021 a         2670 c         2513 g          957 t
ORIGIN      
        1 acgctcagcg tgagcgaggg gatgctgcag gggaccacca cgggaacgat cctcaaggat
       61 cagagcggca cgacgatctc cggcgtgatc tatcccgaca cggccaacgt cacgctcttc
      121 gcctccgcca agcagatcga gccgggctcg tggttcctca gcgtccagaa gagcgcggtg
      181 gccttcggga cctcgccggc cgctttcacg gtgtcgacct gcggcccggg cgatcccagc
      241 ccggccaacc ccaagtgcat cgtcgcggag ttccacaacg gggctccctc cggctacgcg
      301 gcccacgggt cgtgcctcgg atacccgggg gtcctggagg ggacctgccc caacgcggaa
      361 accgcggcgg acgtctcatt gatctcgctg atctcggacc tgggcggcct ctacacgaag
      421 acggatccgc tcaaggtctc gtggttctgt gatcagtcgg cctgccccag gggtaccggc
      481 ggcccgacct cgggcaaccc gttcaccacg ctgttcgtcg aggtggacgg ctcgttcgtg
      541 gcctcgccgg cctgcccgtc gaaggggacg atcgggcccg atcagaagtt ctgcaccgac
      601 tacgtgcaga gctcgggcct ggccgggggc gacctcctcc tggtcatcct cttcgacggg
      661 gatccgcgct ggatgtgacc cgtcgggggc ggggctccgt gccccgcccc cggatcgggg
      721 gcatgaaggc gacgcccagg cgctcgttca gctccccggc ctcctgatcg aaccgctccg
      781 cctcggggtc gccgaggacg cgggcggcgc ctgccagggc cgccagcgcg aacgccacct
      841 cgtcgtcggc accctgcgct cggcccatct cgacggcctc ctccagcgcc gcccgcgctt
      901 cctccgggcg gccgccctcg aggagcgcat acccgcggac ccggtgcagg atcgggagga
      961 ggggctcgcc ctcgatcgtc agagcgtggg agatcgaggt ggtggcgagc tccagcgctc
     1021 cggaccagtc gcctgcaagc aggcgggcct cggcgatggc cgcgtccgtc tccaggacct
     1081 cgttccgggc ccctagggtc gtgaaggcct cccgcgcttc cccgagcagg tcgaggccct
     1141 cccggtgacg acccgcccga gcggccgccc ttcccagctc cctggtggcg aaggcgacgc
     1201 cctcgcggtc gcctactgcg cgagacatcc tgcgggcctc gtggagccga cgctcggcct
     1261 cctcgagctg gccccgccgg accaggaggt cgcccctgtt gaacgccgcg atgatcacgc
     1321 cctgtgagtg cccgatcctc gagaagctct cctccgcccg ctccagcagc tcgagcgcct
     1381 catcccagcg agcctcgagg acggctctga ccccgaggtt gttcagcagg ttggcctgca
     1441 tcgagcgatc gcccaactcc tcgaagatcc ccagcgccac gcgcccgtac gtgtctccgg
     1501 cctctccgcc cgaggaccac tccaccatgt cgagaacgcg gtaggcctct cccagcgccg
     1561 ccttgtcgcc ggcctgctct gcctcggcga ccgctcgctg cgcccatcgg ccggcctcgc
     1621 ggaggcgtcc ctgccggaag cggcacagcg cgtacagcga cgagagcttc gccacggtgg
     1681 cggcggtctc ctcgccgggc aggccctcga gcgtgtggag ggcccgggtc acccatccca
     1741 gcgccctcgg gaagaggccc agccagtgaa gggtctcggc ctccttcagc atgagctgcg
     1801 cgtctcgcac cggcccgccg tcggctcggc gcctagcctc ccgatacgcg cgggccgcgt
     1861 cctcgtacag gccggatcgg ctcttcacct cgcccagggc ctccaaaacc tctgcgatgc
     1921 gtcggggggc gacgccgccc gctctccggg ccgcatcgag cgcccgctga tagaggtcgg
     1981 ctgcctcgac gttggcgtag acggcccggg cctgatcgcc ggcgacggtc gaataccgcc
     2041 aggcctccgg atagcgccca gcgttgaaga agtgcaggga gaggagctcc gcggcctcct
     2101 cgggacggtc gcccgatgca cgctcgatgc tctccgcggc cgacgcgtgg agcgaacgcc
     2161 ggacgcggta gggcaggccc tcgtaggcgg cctcgcggat gagggcgtgc cggaagcgga
     2221 gcgtgccgtt ctcccgctcg acgaactcgc ccagctgctc ccaggcctcc ggacccgcgg
     2281 gatcggccga gagctcctcg accaggctgg gctcgaaggc gagtcccagg accgaggccc
     2341 gtcgcaggag ggcccgatcg acgggatcga gccgatcgat gcgggcggcg atcagcgcct
     2401 cgaccgaatc gggtatcgcc tcgacgtccc cgacccgccg ggcggcggcg aggagctcga
     2461 tgaggaagag ggggttcccg ccggcgcgct cggccaaggc cttcacctcg tgcggccgga
     2521 ggggggactc ctcggaggcc gcggtcagga gcgactgggc cgcggccgca tcgagaggct
     2581 cgggccgcag ggcgatggtg gcgggtccct cgggagctgc gaacgaggag tcgtgctcgc
     2641 gccgggtgat gacgagcagc cacggacggt ccgcgacggt gcggacgagg ttcgagagga
     2701 ggctggcgga ggcctcgtcc atccagtggg cgtcctcgat cgtgatcatc gtcggtccag
     2761 agagcagcgc acccaggagc tcggccgtgg cctgctccgc tcgctcccgg cggaagcgct
     2821 cttcgagctc ctcgacctcg ggggtgggag ggagatcgac gtggagcggg agcgccagga
     2881 gcggcagcca ggggaggagc tggggggcgt gccgctcgac aagccctcgg agggtcgtct
     2941 cgacctcgcg ctcggacgcg tcgtgaggga ccccgagcgc gccctggagg aggtcgcgga
     3001 acggtcggta aggcgtctgc gactcgtaca gctcgcaggt cgccgtgtag accgcggcgt
     3061 caccggcccg atcgcggagc tcccggacga gccgcgactt cccgatgccc gcgtcgccga
     3121 tcacctcgac ggcccggccg gcgcccgccc gggcggacgc gagcccctcg agcagcacgg
     3181 ccatctcgcg ctctcggccc acgaacgggt gctcttggga agacccggcc ttcgccccct
     3241 cggcggctcc cacggtgcag gcggtgatcg gcgcctcctt gcccttgacg cggaacgccg
     3301 ggagccgctc ggcgcggaag atcgtgcgcg accggcggag gacccacctg ctcgagatca
     3361 cctgccccgg ttctgccctc gccatcagcc tcgccgcgag gttcaccgcg tcgcccatga
     3421 cggtgtaggt ccgccggtag gcggggccga tgtcgccggc gaagactcgc cctccgtgca
     3481 ccccgatcct gatcgggagc ggcaggcctg cctccgcgat ctcgcgcacc gcgagcagga
     3541 tccgctcctc gtcgttcccc accgtccggg gcgcgccgga ggccaggatg atcttccccc
     3601 cgtcgcggtc gacatcggtg cccaggaagg tcacgtcgtg ccggtcgacg gcgcgctgga
     3661 ccccctccac cacggccgcc agcgccgtgg ccgccgcctt cggcccctcc tcctcgacca
     3721 cgcggtcgat gccctcgaac cgcaggaagg cgacggtgac caggcggtgc tcccggtcga
     3781 gggagcctcc gagcacggcc ttccgcacgg cgaccgggag gcagcgggcc agcgcctcgg
     3841 gggggcccgt gacgtcgaac gacgggaggc cgctcgggga cccgggaggc cggcgccgaa
     3901 ggaggcttcc ggggcccttg gcctcgccca gcaggcgttc cggcagcgcg gcggccgcgc
     3961 cggggctcag cacgatctcc cctgcctcgg cggcggtctc catggccacg gcctcggtgg
     4021 tgcccgggcc tgccagcacg agctcgcggt gggactcgcc caccaggaag aagtggaagg
     4081 tcccgctgtg cacgccgatc gacatccgga gggtcacccg tccccggggc gtccggatcg
     4141 cacccatctc gcggagcgcg cgacgcatcc ccgcggcggc gcgactggcc cggaccgcat
     4201 gcccgtcgtc ctcgaagagg aggagcaggg cgtcgccccc gaacttgatc aggctgccgc
     4261 cctcctcgta ggccacggcc agcagcgtgg tgaacgagga gtcgatcgcc tccgcgacct
     4321 cctccgcgcc cagcttcccg agctcggcga gccgctcgga gagcctggtg aacccagaga
     4381 tgtcggcgaa agcgacggac ccggccacct ggcggctcgc ctcgcccggc gactcctcga
     4441 tccaccgcag gaggaggcgg ggcaggtagg gacggaggcg ctcgcgggcg tcggtgacgg
     4501 tccccggcat caccacctgt tccgcgtctc ctcccgggcg ctctcggtcc gcctcgtgcc
     4561 cacgcgggat gggccgtcga cgccgcaacc ctcgttgggc ggcctcgctg tatcaactgt
     4621 gccccatcgg gcctcgtcgc gccatggtca cacgtgtccg tccgccctcg ccgcgagcgc
     4681 cctctcccgc taggctgtgc tcgtggccct ggaccgtcaa ctcgagggga tcgccggcaa
     4741 gctgcaggac gagatcgtcg tgtggctgac gaccgtctcg ccggaggggc agccgcagag
     4801 cgtcccggtc tggttcctct ggcgcgacgg cgccttcctc atctacagcc agccggacaa
     4861 gccgaagctc cggaacctgg cagccaaccc ccgggtctcg ctccacttcc ggggcacccg
     4921 gaacggcgag gacatcgcca cgctcgaggg ggaggcccgg cgggccgagg accatccgcc
     4981 cgccgaccag gtccctccct acgtcgagaa gtacgccgcg gagatcgaga agctccgatg
     5041 gaccccccag agcttcgccg ccgactactc gcagccgatc ctcgtcaggc ccacgcacgt
     5101 cctgacctgg tgatctagac gcgacgccgc agcaggaggc ccgccagcag ggccgaccct
     5161 acgagccagg ccaggacgat gagcgtctgg atgccgttcc agccgaaacc gagccacgga
     5221 tcctgcatca ggaggatgac gtgccgcagc ggcgtggcat cggccaccgt cctcgtggcg
     5281 tcggtgagga cctcgggcgg ggggccggcc cccgcgatga tgagcatcac gaaccaaagg
     5341 aggactcccg cgccctgcgc cgctcgcgtc gtcggcatcg tcgccccgag cagcccaccg
     5401 atggcggcga aggtcagagc gcccagcgcg aacgcgccga cgaacagtgc gggggagaca
     5461 ggcgcggcga tgtcgtatga ggccgacgcc acggcgatca gcagggcgct cccgaccgcc
     5521 gaggccacca ccgcgacgag cgcgtgagcg gcgaagaccg tccacagcgg gaccgtggag
     5581 gcccggaacc ggcgcaggat gccccgctcc cgatactcgg ccaggtgcgt ggggagggcg
     5641 atcaggccca gggaggcggt caccagcgcg gcgtaggccg gcacgtagta gtcgatcggc
     5701 cccacgcccc gatacacgtc cgcttcgggc gtgttgccga acacgctggc caggacgaag
     5761 agcatcacga tcggcagcgc gagcgtgaag acgacggtga gcggctcgcg ggcgaagagc
     5821 ttcatctcga tccaccaggt gcggaggagc gccctcatca ccccatcccc tcgctcgtgc
     5881 cgacgagcgc caggaagacc tcctccaggg tggagcgctc gaggcggagg tcctcgggct
     5941 cgatcccgcg ggtcgagagc gcggcggcca cccgggccag caccgccccg gacccgtcca
     6001 cggcgacctc gctcccgcga gacgccacgg aggtcacgcc gtcgatggcc tcgaggaacg
     6061 acgcgtcgcg cagctcggcc gtgaaccgca cccggacggc gccggcgtgc cgcacgatga
     6121 gctcttgggg cgatcccatc gcgaccaccc ggccccggtc gacgatcgcg acccggtcgc
     6181 agagccgctc ggcctcgtcc atgaagtgcg tcacgaggac gacagtcgtg ccgcgctctc
     6241 ggatcgccga gatcagctgc cacgccaccc gtcgcgcccc cgggtcgagg cccgtggtca
     6301 tctcgtcgag gaacacgacc tcggggtggt tgagcagggc cagggcgatg aacagtcgct
     6361 ggcgctggcc tccggagagg gcctggaacc tggcgccccg cttctgggcc agcccccact
     6421 ccacgaggag gcgctcggcc tccgccccgt cggcccccga accgaacagg tggagcgcct
     6481 cgcgcacctt gatccggtcg gggagcgccg actgctggag ctggctcccg atccgccgcc
     6541 gcagctcggc ggcctggcgc gtggggtcga ggccgaggac gcggagcgat ccgcggtcgg
     6601 gcgcgcgcag gccctggagg cactccacgg tggtggtctt ccccgcgccg ttggggccga
     6661 ggaggccgaa gatctcgccg cgccgcacgt cgaagctgac gtcctcgacc gcgaccgcgt
     6721 ccccgtaccg cttgtgcagc gcgtcgacca ggacgacggg ctcggccggg ctcactccgg
     6781 cccgaccatc cgctcggggc ggaccgcctc gtcgtactcc tccccggtga ggtacccgag
     6841 cgagagggcg gcctccttca gcgtcgagcc ttcggcgtgc gccttcttcg cgatctcggc
     6901 ggccttgtcg tagccgatct tcggcgtgag ggcggtcacg agcatcagcg atcgatcgac
     6961 gagctcggcg atccgctcgc ggttcggccg gatcccatcg acgcagaact cgcggaacgc
     7021 ccccatcgcg tcggccagga tcgccaccga gtgcaggaag ttccggatca tcaccggctt
     7081 gaagacgttg agctcgaagt tgccctggga gccggcgatc gccacggccg cgtcgttccc
     7141 catcacctgg acgcagacca t
//
LOCUS       DASUTZ010000063         9256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1441763, whole genome shotgun sequence.
ACCESSION   DASUTZ010000063 DASUTZ010000000
VERSION     DASUTZ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9256
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441763"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..700)
                     /locus_tag="VF097_02895"
     CDS             complement(53..700)
                     /locus_tag="VF097_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX6261777.1"
                     /translation="MAVARGLLIDFGGVLTTPLGDALRRWCEREGVDADRLRELLQSS
                     YPVTDPDSIVIQVETGRMPPEEFERRLAEVFSEGLHRPLEPDGLLERMGEAIELEPRM
                     IDAVRIAHAAGVRTALISNSWGLHYYPHDLLAELFDEILISGQVGLRKPEDEIYLLAA
                     RGLGLEPPECVFVDDVEINLHTAERLGMRTLLHEEPERTIVELEALLGVPLGDAR"
     gene            complement(691..1557)
                     /locus_tag="VF097_02900"
     CDS             complement(691..1557)
                     /locus_tag="VF097_02900"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HEX6261778.1"
                     /translation="MRARELVREGERRLKRSPAIDHWPADRERREAAALLAKALGHDE
                     EEPEDLDASVSGRAAARFRRLVRRRAGGEPMGHILGWTEFRGLRLRVRPGAFVPRQSS
                     EWTVEQTVRRLRPRPRPVHVDVATGIGPIALAVASEVPKAEVHGTDLMPEGLRQARRN
                     ARALGIRNASFHVGDLLDPLPGRLRGKVDAVTGHIPYVPLAEVDDLPLEVRGFEPEAT
                     LTDYSDGMSLLERAVLEACGWLRPGGWLLMEVSPDRSRAVKALMQRAGYAEVRSTKGW
                     PEISRTVVGRWR"
     gene            1592..1945
                     /locus_tag="VF097_02905"
     CDS             1592..1945
                     /locus_tag="VF097_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX6261779.1"
                     /translation="MQAKPVHPVPEGESLKAVRMMNDRVLVEPPASDEERRSRGGLVI
                     PATAASLNRKGVWGRVVAVGPNARTVEVGDEVLLLPESGIEVEIRGRDYVLVREREIH
                     AVATEREDPGSGLYL"
     gene            complement(1999..2793)
                     /locus_tag="VF097_02910"
     CDS             complement(1999..2793)
                     /locus_tag="VF097_02910"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6261780.1"
                     /translation="MGEWVESRGVRLAVEVLGEGEPVSVVAHGLTQSRHELSLFAPFV
                     PGTKVLFDFRGHGESERPGPGTYRMDDFAADVEAVADAYGATVLIGASLGGGATLRLL
                     RRRPDRFERMVILLPARLERGVGSEGARARLLRVAEHLERYPIEEAAERILAAEEAEG
                     TFDGTEAARDLRRRALLRMNREGMPHAIREILDDPPVTDPAPLRLVRAPALVIGQEGD
                     PVHESWVARELAETLPKGELMLFPDPFAMVQEIPALVSRIGSFIAG"
     gene            2853..3845
                     /gene="speB"
                     /locus_tag="VF097_02915"
     CDS             2853..3845
                     /gene="speB"
                     /locus_tag="VF097_02915"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006542321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEX6261781.1"
                     /translation="MDVTRPNEPAAGSSGATFSRLPLVLDPDELRGADVAILGAPIDE
                     TVSYRPGARFGPRAIRLADVSGGSPPRFPHMELGIDPVQELTIVDHGDIEAVPGGQER
                     THAILREAVGRVLQAGCVPVILGGDHSILHPDAGAVADHHGPGTVGVIHFDAHADDAE
                     EIWGVRLSHGTPVRRLVEEGSVPGDRILQIGLRGYWPGPEEFAWAREQGLVWYTQGEI
                     DERGLRAVLDDVVERARAWEHVYLSLDIDVVDAGSAPGTGTPEPGGLTPRELLTAVRR
                     LCVELGFAGMEVVEVSPPYDHADVTALLAHRAVLEALSGLALRKAGREPRPERP"
     gene            3910..5865
                     /locus_tag="VF097_02920"
     CDS             3910..5865
                     /locus_tag="VF097_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6261782.1"
                     /translation="MDTKSLEELLAEGRDAAARHAWREARELLEAADAVEPLTASDAE
                     VLARSAWWLGDLAACIGLRERAHRAYKKAGQILGAARVALDLSHDYLMKGDAAMGSGW
                     HRRAERLLADQAPAPEHGYLERMRASAAMAAGDLDGALEHAIRCEELGERFADPDLQA
                     LGLHEQGRILIRQGRAPEGLAALDEAIVAAVSGELDPVVTGIIYCNAIEICRDLTDYA
                     RAGEWNDAAKRWCERMAVSGFPGICGVRRAEIGKLRGAWPEAERQARQAVSELKGWYV
                     DVAAAGLYEIGEIRLRMGDHAAAEEAFRDANGLGHDPQPGLALLRLREGEVGAAWTAI
                     QRSLAATSDADRLRRARLLPAAVEIALENGDVGAASGAAEELEAIAETFLSTALRAAA
                     RVAQGRVLLAGGEARKAGEALREGRGLWAEVEAPYEVAEARRLLGLAYREAGDEDGAR
                     LELEAALTAFETIGGAPDAARVAAELGLAERRASPGRAVRTLMFTDIVGSTGLIEAIG
                     DDAWSDLQRWHDETLRAAFAQHGGEEVDHAGDGFFVAFHDPASAIECAVAIQRLLAQH
                     RRTSGFAPRVRIGLHTTQASTKPGGYGGRGVHEAARIGSLGGADEIVVSRGSLPEHTR
                     FPVSEPRSAELKGIEGPVEVVTVDWRP"
     gene            complement(5869..6258)
                     /locus_tag="VF097_02925"
     CDS             complement(5869..6258)
                     /locus_tag="VF097_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6261783.1"
                     /translation="MDVERVKSIPLFADLSQSERELVARQADEVEVEAGRHLIEEGEI
                     GWEFFVIESGAVEVKVGDDPVATLGPGDFFGELALKDQTHRRTASVVTTEPSTLIVMT
                     GQAFRTVEGDMPSVAERIREAVERYGN"
     gene            6348..8243
                     /locus_tag="VF097_02930"
     CDS             6348..8243
                     /locus_tag="VF097_02930"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX6261784.1"
                     /translation="MRRLGSLLAALALSLSPIAPAAGQGVEVSAIDDVFRPETIRIPV
                     GGTVTWINEGRNPHTATADDGAWDSGNLDPGDTSSLRFDEPGAIPYFCRYHGAAGGLG
                     MAGTILVGDVQSPEAQEGPPQPPPGEGDTLRVPADHPTIQAAVNAAVPGDLVLVSPGV
                     YRESVRVTTPFLTIRGTDRNEVIVDGEFSRENGVHVLEADGVAVENMTARNHTLNGFY
                     WTGVEGYRGSYLTAYNNGDYGIYAFDSVYGQFDHSYASGSPDAGFYIGQCRPCHAVIS
                     DVVAEGNALGYSGTNAGGKLYIVRSEWRNNMSGIVPNTLDSQLLAPQGEVVIAGNWIH
                     DNNNREAPAKRLQYPTFGSGVLLAGGTDNLVVRNRVEGHERYGIAAVPNLDANLWVTE
                     GNEVRANLVSGSGEADLALGGPAAGRDCFAENDHRRSLPPAIEALAGCGLRLTGGAGD
                     LGVTFSLLGRFAQALSGDFPQGDYRTAPVPPPQETMPDAETAPPAPAVDVPMKIDVAG
                     VALPPGGSATPARHTEVTVLGISVAAPTWWGLLISVYGYLLPLVLYAAWVSVAAWDLV
                     RRDGPTTRARIGWLAVVLLVPFLGPILYFVFGRSPIPAALRWMLVGGGLGAYLIFAGI
                     SFLLASS"
     gene            complement(8251..9168)
                     /locus_tag="VF097_02935"
     CDS             complement(8251..9168)
                     /locus_tag="VF097_02935"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6261785.1"
                     /translation="MQQRERLSAAADAVGGVAAAASAFAFGSAVVLGVVATERGLPIP
                     GLLAIRFTIAAAAVALVLASLRRSLRAAPGEGWWLLLLGGIGYGAEAGFFYLALQHGT
                     AAAVSLLFFTYPVWVTVFVALMGRGGPGLLVGGSLAAAVAGAALVVASSGGLDIDLTG
                     ILLSFASAVTFAVYLILVDWRLRRTPSLVASMWVTAGAAVALALYALVSGEGRMPRDR
                     TEWWAVVGQGVVTSAAFLGLFIGLKRLGPVRVSIVASLEPMATAILAFLVLGQAVRPL
                     TALGGALIMGAAVTAALARGREKLEQTPP"
BASE COUNT         1317 a         3431 c         3316 g         1192 t
ORIGIN      
        1 ggcgacgccc ttcggcgtgg tcagatcagg ttccatccgg acagcgatcg tatcaacgag
       61 cgtcgccgag cggcaccccc agcagggcct cgagctcgac gatcgtgcgc tcgggctcct
      121 cgtgcagcag cgtgcgcatc cccagccgct cggccgtgtg gaggttgatc tcgacgtcgt
      181 ccacgaagac gcattccggc ggctcgaggc cgagaccacg ggccgcgagc aggtagatct
      241 cgtcctcggg cttgcgcagg cccacctggc ccgagatgag gatctcgtcg aacagctcgg
      301 ccagcaggtc gtgcgggtag tagtgcagtc cccacgagtt cgagatcagc gccgtgcgca
      361 cccccgcggc gtgcgcgatc cgtaccgcgt cgatcatcct cggctcgagc tcgatcgcct
      421 cgcccatacg ctccaggagc ccgtccggct cgagcggccg gtgcaggccc tcggagaaga
      481 cctcggccag gcgccgctcg aactcctcgg gcggcatccg gcccgtctcg acctggatca
      541 cgatcgagtc gggatcggtc acggggtacg aactctgcaa cagctcgcgg agccggtcgg
      601 cgtccacgcc ctcgcgctcg caccatcgcc gcagcgcgtc cccgagcggt gtggtcagca
      661 ccccgccgaa gtcgatcagc aggccccgcg ctaccgccat cggcccacca ccgtccggga
      721 gatctccggc cagcccttcg tgctccgcac ctccgcgtac cccgcgcgct gcatcaaggc
      781 cttgaccgcg cgggagcggt cggggctcac ttccatgagg agccagccgc cggggcgaag
      841 ccacccgcat gcctcgagga ccgcgcgctc cagcagcgac atcccatcgg agtagtcggt
      901 gagcgtggcc tcgggctcga accctcggac ctcgagcggg aggtcgtcca cctccgccag
      961 gggaacgtac gggatgtggc ccgtgaccgc gtccaccttc cctctgagcc gtcccgggag
     1021 cgggtccagg aggtccccga cgtggaagct cgcgttgcgg atcccgagcg cccgtgcgtt
     1081 gcgccgggcc tggcgcagcc cctcgggcat cagatccgtc ccgtgaacct cggccttcgg
     1141 gacctcgctc gccaccgcga gggcgatcgg gccgatcccg gtggccacgt ccacgtgcac
     1201 gggtcgcggt cgtgggcgga gccgccggac cgtctgctcc acggtccact cgctcgactg
     1261 gcgggggacg aaggcgcccg gacggacccg gagccggagc ccccggaact cggtccatcc
     1321 gaggatgtgc cccatcggct cgccgccggc gcggcgccgg accagccgcc ggaagcgggc
     1381 ggcagcccgc cccgacaccg acgcgtcgag gtcctcgggc tcctcctcgt cgtgccccag
     1441 cgccttcgcc agcagcgcgg ccgcctcccg ccgctcccgg tcggcgggcc agtgatcgat
     1501 cgccggcgac cgcttcagcc gccgctcgcc ttcccgaacc agctcccgtg cgcgcatcga
     1561 cgatgaacga taccgagtat cctgtcctgc gatgcaggcg aagccggtcc accccgtccc
     1621 cgagggcgag tcgctgaagg cggtccggat gatgaacgac cgcgtcctcg tcgagccgcc
     1681 cgccagcgac gaggagcggc ggtcgcgcgg cgggctggtg atcccggcca ccgccgcgtc
     1741 gctcaacagg aagggcgtgt ggggccgcgt ggtggccgtc gggccgaacg cccggacggt
     1801 cgaggtgggc gacgaggtgc tgctcctccc ggagtcgggc atcgaggtcg agatccgcgg
     1861 ccgcgactac gtgctggtga gggagcgcga gatccacgcg gtggccaccg agcgcgaaga
     1921 ccccggctcg ggcctgtacc tctagcgctc cgagcaggca cccgtccgac gagacctcgt
     1981 ggatgccccg cagggccctc agccggcgat gaacgacccg atgcgactca ccagcgccgg
     2041 gatctcctgc accatggcga acgggtcggg aaacagcatc agctccccct tgggcagcgt
     2101 ctccgccagc tctcgcgcca cccaggactc gtgcacggga tccccctcct gtccgatcac
     2161 cagcgccggg gctcgcacga gccgcagggg ggccggatcg gtgaccggtg gatcgtcgag
     2221 gatctcgcgg atcgcgtgcg gcatcccctc gcggttcatc cgaagcagcg cccggcgtcg
     2281 aaggtctctg gccgcctccg tcccgtcgaa ggttccctcc gcctcctccg ccgcgaggat
     2341 ccgctccgcc gcctcctcga tcgggtaccg ctccaggtgc tccgccaccc ggagcaggcg
     2401 ggctcgggcg ccttcggacc ccacgccccg ctccagccgc gccggcagca ggatcaccat
     2461 ccgctcgaac cgatcgggtc tccgtcggag gagccgcagg gtcgctcccc cgcccagcga
     2521 cgctccgatc agcaccgtgg ccccgtacgc gtcggccacc gcctcgacgt cggccgcgaa
     2581 gtcgtccatc cgataggtgc ccggcccggg gcgctcgctc tcgccgtgcc cccggaagtc
     2641 gaagagaacc ttcgtgcccg gcacgaacgg cgcgaacagg ctcagctcgt gtctcgactg
     2701 cgtcagcccg tgcgccacga ccgagaccgg ctccccctcg cccaggacct ccacggcgag
     2761 gcggacacct cgcgactcga cccactcgcc catggcgggg acgataccag ccgcttgcgc
     2821 gcgcgggtcc gagcggcgag gctggcctcg ccgtggacgt cacgagaccg aacgagccgg
     2881 ccgccggatc cagcggggcc accttctccc gcctcccgct ggtcctcgat cccgacgagc
     2941 tgcggggcgc cgacgtggcg atcctgggcg cgccgatcga cgagacggtc agctaccgcc
     3001 cgggggcgcg cttcgggccg cgggcgatcc ggctggccga cgtcagcgga ggatcgccgc
     3061 cgcggttccc ccacatggag ctggggatcg accccgtcca agagctcacg atcgtggacc
     3121 acggcgacat cgaggcggtc cccggcggac aggagcgtac ccatgcgatc ctccgggagg
     3181 ccgtgggtcg ggtgctccag gccggctgtg tccccgtgat cctcgggggc gaccactcca
     3241 tcctccatcc ggacgccggc gccgtggccg accaccacgg ccccggcacc gtgggcgtga
     3301 tccacttcga cgctcacgcc gacgacgccg aggagatctg gggcgtgcga ctctcgcacg
     3361 ggacgccggt caggaggctg gtcgaggagg gctcggtccc cggcgaccgg atcctccaga
     3421 ttggcctccg cggctactgg cccggcccgg aggagttcgc ctgggcccga gagcagggcc
     3481 tcgtctggta cacgcagggg gagatcgacg agcgagggct gcgagcggtc ctggacgacg
     3541 tcgtggagcg ggcgcgggcc tgggagcacg tgtacctctc gctcgacatc gacgtggttg
     3601 acgcgggatc ggcgccgggg accggcaccc ccgagccggg cgggctcacc ccccgcgagc
     3661 tcctgacggc tgtccgccgc ctctgcgtcg agctgggctt cgccggcatg gaggtcgtcg
     3721 aagtgtcgcc gccctacgac cacgccgacg tgacggcgct gctggcccac cgagccgtcc
     3781 tggaggcgct ctcgggcctg gccctccgga aggcggggcg ggaaccgcgt cccgagcggc
     3841 cctgagccgg gacgggcgac ggaaaccctg ggaagcccac ctccgccccg ggcgtgggac
     3901 catggcctgg tggacacgaa gtcgctcgag gagctcctcg ccgaggggcg cgacgccgcg
     3961 gctcggcacg cctggcgcga ggcccgcgag ctgctggagg cggccgacgc cgtggagccg
     4021 ctcacggcct ccgacgccga ggtcctggcg aggtccgcgt ggtggctcgg ggacctcgcc
     4081 gcctgcatcg gcctccgaga gcgggcccac agggcgtata agaaggcggg ccagatcctc
     4141 ggagcggccc gcgtggctct cgacctgagc cacgactacc tgatgaaggg cgacgccgcg
     4201 atggggagcg gatggcaccg gcgggcggag cgactgctgg cggaccaggc ccccgccccc
     4261 gagcatgggt acctcgagcg gatgcgagcg agcgccgcga tggccgcggg ggacctggac
     4321 ggggccttgg agcacgcgat ccgctgcgag gagctcggcg agcggttcgc cgaccccgac
     4381 ctccaggccc tcgggctcca cgagcaggga cggatcctga tccgccaggg acgcgccccg
     4441 gaggggctcg cggccctcga cgaggcgatc gtggccgccg tgagcggcga gctcgacccg
     4501 gtcgtgacgg ggatcatcta ctgcaacgcg atcgagatct gccgcgacct taccgactac
     4561 gcgcgggcgg gcgagtggaa cgatgcggcc aagcggtggt gcgagcggat ggccgtctcg
     4621 ggcttccccg ggatctgcgg ggtccgacgg gcggagatcg gcaagctgcg cggggcgtgg
     4681 ccggaggcgg agcggcaggc tcgccaggcc gtctccgagc tgaaggggtg gtacgtggac
     4741 gtcgcggccg ccggcctcta cgagatcggg gagatccgcc tccgcatggg cgaccacgcc
     4801 gcggccgagg aggccttccg cgacgcgaac ggcctggggc acgacccgca gcccggcttg
     4861 gcgctcctcc gcctgcgcga gggagaagtc ggcgcggcat ggacggcgat ccagcggtcc
     4921 ctggccgcga cctcggatgc cgaccggctc cgccgcgccc gactactgcc ggcggctgtc
     4981 gagatcgccc tcgagaacgg cgatgtggga gccgcctcag gagccgccga ggagctcgag
     5041 gcgatcgccg agaccttcct ctccacggcc ttgcgggcgg cggcgagggt cgcgcagggc
     5101 cgggtcctcc tcgccggggg ggaggcccgc aaggcgggcg aggcgctccg ggaggggcgt
     5161 ggcctctggg ccgaggttga ggcgccgtac gaggtggcgg aggcgcgccg gctcctcggg
     5221 ctggcctacc gtgaagccgg cgacgaggac ggggcgcggc tcgagctgga ggccgcactc
     5281 acggccttcg agacgattgg gggcgctccc gatgccgccc gcgtcgccgc ggagctcggg
     5341 ctcgcggagc ggcgggcgtc tccgggccgg gccgtccgca cgctcatgtt caccgacatc
     5401 gtcggctcga cgggtctgat cgaggccatc ggggacgacg cgtggagcga cctgcagcgc
     5461 tggcacgacg agaccctgcg cgccgcgttc gcgcagcacg ggggcgagga ggtggaccac
     5521 gccggggacg gcttcttcgt cgcgttccac gacccggcat ccgcgatcga gtgcgcggtc
     5581 gcgatccagc gtttgctggc gcagcaccgg cggacgagcg gcttcgcgcc tcgggtccgt
     5641 atcggcctcc acacgaccca ggcgtcgacg aagccgggag gctacggggg gcgaggcgtt
     5701 cacgaggccg cacggatcgg ctctctcgga ggggcggacg agatcgtggt cagtcgaggg
     5761 tctctccccg agcacacgcg cttcccggtc agcgagccac gatcagccga gctcaagggg
     5821 atcgagggac cggtcgaggt cgtgaccgtc gactggcggc cctgacattc agttgccgta
     5881 gcgctcgacc gcctcccgga tccgctcggc aaccgacggc atgtcgccct cgacggtgcg
     5941 gaaagcctgt cccgtcatca caatcagtgt cgagggctcc gtcgtgacca ccgacgccgt
     6001 ccggcgatgc gtctggtcct tcagcgcgag ctcgccgaag aagtcccccg ggcccagggt
     6061 ggccacggga tcgtccccga ccttgacctc gaccgcgccc gactcgatca cgaagaactc
     6121 ccagccgatc tcgccctcct cgatgaggtg cctccccgcc tcgacctcca cctcgtcggc
     6181 ctggcgggcg accagctcgc gctcgctctg cgagaggtcg gcgaacagcg ggatcgactt
     6241 cacacgctcg acgtccatgc agcctccctc gtcgtggttc ctgggtatct ttccagcatc
     6301 cgacccgggc ggccactcgt gagcgggcct ctggtaggct cccgccgatg cgccggctcg
     6361 gttcgctcct cgcggctctc gcgctgagcc tgagccccat cgctccggcc gcggggcagg
     6421 gagtcgaggt ctcggcgatc gacgacgtct tccgccccga gacgatccgg atccccgtgg
     6481 gcggcacggt gacctggatc aacgagggtc ggaacccgca cacggcgacg gccgacgacg
     6541 gggcctggga ctccgggaac ctcgacccgg gcgatacgtc ctccctgagg ttcgacgagc
     6601 cgggagcgat cccctacttc tgccggtacc acggggcagc cggggggctc ggcatggcgg
     6661 gcacgatcct cgtgggagac gtgcagtccc cagaggccca ggagggccct ccgcagcccc
     6721 cgccgggcga gggggacacc cttcgggtgc ccgcggacca tcccacgatc caggcggcgg
     6781 tgaacgccgc ggtgcccggc gacctggtcc tggtctcccc cggcgtctac cgcgagagcg
     6841 tccgggtcac cacgccgttc ctcacgatca gaggcaccga ccggaacgag gtgatcgtcg
     6901 acggggagtt ctcccgcgag aacggcgtcc acgtgctgga ggccgacggc gtcgcggtgg
     6961 agaacatgac cgcccggaac cacaccctga acggtttcta ctggaccggg gtcgagggct
     7021 accggggctc gtacctgacg gcctacaaca acggggacta cgggatctac gccttcgact
     7081 cggtgtacgg gcagttcgat cactcctacg cgtcgggcag ccccgacgcg ggcttctaca
     7141 tcgggcagtg ccggccctgc catgccgtga tcagcgacgt ggtggccgag gggaacgccc
     7201 tcggctactc ggggacgaac gcaggcggca agctgtacat cgtgcggtcg gagtggcgga
     7261 acaacatgtc ggggatcgtc cccaacacgc tcgactccca gctgctggcc ccacagggcg
     7321 aggtcgtgat cgccgggaac tggatccacg acaacaacaa ccgggaggcg ccggcgaagc
     7381 ggctccagta cccgaccttc gggagcgggg tgctgctggc cggggggacc gacaatctgg
     7441 tggtgcggaa ccgggtcgag gggcacgagc ggtacgggat cgccgccgtg ccgaacctcg
     7501 acgcgaacct gtgggtcacc gaggggaacg aggtgcgagc gaacctcgtg agcggttcgg
     7561 gggaggccga tctggcgctg ggcgggccgg cggcaggccg ggactgcttc gcggagaacg
     7621 accacagaag gagcctgccg ccggccatcg aggcgctcgc cgggtgcggg ctgcggctga
     7681 cgggcggggc gggggacctc ggggtcacgt tctcgctgct ggggaggttc gcgcaggcac
     7741 tgtcgggcga cttccctcag ggcgactacc ggaccgcccc cgtgcccccg ccccaggaga
     7801 ccatgcccga cgcggagacg gccccgccgg ccccggccgt cgacgtcccg atgaagatcg
     7861 atgtggcggg ggtcgcgcta ccgcccggcg gcagcgcgac ccccgcccgg cacacggagg
     7921 tgaccgtgct cggcatcagc gtcgcggccc ccacctggtg gggcctgctc atctcggtct
     7981 acggatacct gctcccgctc gtcctgtacg cggcctgggt ctcggtcgcc gcgtgggacc
     8041 tcgtgcgccg cgacggcccg acgacccgag cacgcatcgg ctggctggcc gtcgtcctgc
     8101 tcgtgccctt cctcggcccg atcctctact tcgtcttcgg ccggtccccg atcccggcgg
     8161 cgctgcgctg gatgctcgtg ggtgggggcc tcggcgcgta cctgatcttc gccgggatct
     8221 cgttcctcct ggccagctcc tagcgggcct tcacgggggc gtctgctcca gcttctcccg
     8281 gccgcgggcc agcgccgcgg tcaccgcggc ccccatgatg agcgccccgc ccaaggccgt
     8341 gagcgggcgc accgcctgcc ccagcacgag gaacgccagg atcgccgtcg ccatcggctc
     8401 cagcgacgcg acgatcgaga cgcggaccgg ccccagccgc ttcaagccga tgaagagccc
     8461 caggaacgcc gcggaggtca cgaccccctg gccgacgacc gcccaccatt cggtccgatc
     8521 ccgcggcatc ctcccctccc cgctcacgag cgcgtagagg gccagcgcga cggcggcgcc
     8581 ggccgttacc cacatcgatg cgacgagcga aggcgtccgc cgcagacgcc agtccacgag
     8641 gatcaggtag acggcgaagg tcaccgccga ggcgaaggac aggaggatgc cggtgaggtc
     8701 gatgtccagc ccacccgagg aagccaccac gagcgccgcg cccgccaccg ccgcggccag
     8761 cgaccccccg acgagcaggc ccgggccgcc ccgccccatc agcgccacga agaccgtcac
     8821 ccacacggga tacgtgaaga agaggaggga gaccgccgcg gccgtcccgt gctggagcgc
     8881 caggtagaag aacccggcct cggccccgta cccgatccca ccgaggagga ggagccacca
     8941 cccctccccc ggtgcggcgc gcagcgagcg gcgcagagag gccagcacca gcgcgacggc
     9001 cgccgccgcg atcgtgaagc ggatcgcgag gaggcccggg atcggcaggc cccgctcggt
     9061 ggccaccacg cccagcacga cggccgagcc gaaagcgaac gccgacgcgg cggcggcgac
     9121 cccgcccacc gcatcggccg ccgccgagag tcgctctcgc tgctgcacgc tcccccttcc
     9181 tcgggcgcgg gaggtcccgc ggccgtgtgc tacagttgcc cgccggaggc cgtccggcct
     9241 cgttaggaac ccacat
//
LOCUS       DASUTZ010000064         6024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1052474, whole genome shotgun sequence.
ACCESSION   DASUTZ010000064 DASUTZ010000000
VERSION     DASUTZ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052474"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="VF097_02940"
     CDS             complement(<1..1049)
                     /locus_tag="VF097_02940"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX6261786.1"
                     /translation="MPDLRPMLEARAVAVVGASARPGSVGEQMMRQLLVGGFEGEVHA
                     VNPKYDEVLGVPCVPSLTDVPGGVDLALLGVANERLEDQLRLAAEAGVRSVVIFASGY
                     EESPGDPPLLERWRSIVDESGIAVCGGNCMGFVNVERRLRAVAFAEREDLEPGPITFL
                     SHSGSVFSALLRNDRGLRFNLAVSAGQELTTTVSDYLLYALDLPSTKVAALFIETVRD
                     PERFREALDRAEERDVPVVVLKVGRESATRDLIEAHSGALAGEDGAYEAVFEAHGALR
                     VETLDEMADALELFVSGRRARSGGLAAIHDSGGERVHLIDRAAVAGVRFAQISDRTKA
                     RLTELLDPGLPAVNPL"
     gene            complement(1049..2659)
                     /locus_tag="VF097_02945"
     CDS             complement(1049..2659)
                     /locus_tag="VF097_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013017245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6261787.1"
                     /translation="MRYDAIVIGGGHNGLAAAAYLARLGAKTVVLEARHKTGGAAATD
                     APWPEAPDLKVTTYSYVMSLMPERIIKDLRLRRHGYTVHPMGPYFLAFPDGGSLTFAG
                     PDESLDRESLARFSKRDADAYPEWRAWLAGLADVLAPLLLTTPPRVGSMRPGQLRRLL
                     ATAWRFRGLGVRGVGDVTRLMTMSISDLLDDWFESPQIKGALAIDGIIGTWAGPAAPG
                     TAYVLAHHEIGDAGGGLGTWGFPEGGMGAVADAIRSSAESFGAEVRTGARVARILTTN
                     GRAQGVALESGEELHSDLVVASTHPRITFLDQLERSELPEDFVRDIEQWNSRSGTVKI
                     NLALAELPRFTAKPEPGDHMGGAIELAHSIEYLEEAFQDARAGRPAARPFSDGVIPTV
                     YDRSLAPEGTHIMSLFTQWVPHQWAAEPHRDELEAYADRVIDGYAELAPNLKGAILHR
                     QVLGPYDMEQELGLIGGNIFHGELSADQLFHMRPAPGYADYRTPIRGLYQCSSATHAG
                     GGVTAIPALNCVREIRRDRARLRLRRAR"
     gene            2838..3482
                     /locus_tag="VF097_02950"
     CDS             2838..3482
                     /locus_tag="VF097_02950"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX6261788.1"
                     /translation="MEILEAAVEMIVERGLPETRVADVAERAGTSAALVLYYFESKDR
                     LLTEALSYVEDRFYLQAFHDLTAIDSARERLAVLIARSCPPTGGDVHGDWRLWFELWT
                     RALRDPETARKRAALDRRWRATIADVIRDGQRAGEFGANDPDDLALELASLIDGLAIQ
                     VVLEDPEVDPERMRRICLETAGRALGVDLSVHDLAPSTRAAPRTARAHVSASEE"
     gene            3541..4800
                     /locus_tag="VF097_02955"
     CDS             3541..4800
                     /locus_tag="VF097_02955"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6261789.1"
                     /translation="MDELWGRGMRRRDFLRLSGGSALAAVLAACGGGNGSSGGGGGGT
                     QTEVQVGTPENPVTHPITDDNPMIESGLEPEAGPLRIYNWADYIWPRVLKDFAEEYNV
                     EFELTTFYNLEEALRKLRTEDISFDIFFPTAENIPKVIAGGLLQPLNHDYLPNLQANI
                     WPRLADPFYDSGSRYTVPYAVYQTGIGWRTDMIDTDIESMENPWLAFQDPAHRDITGL
                     YDDYRETMGAGVYMAGSTDINTDDPSLIEESKQNLIRAIQDVNVAFTIDGAYIQLPEA
                     QLGLHHAWSGDLAAAPYYAPKGVDPTALRYVWPPKGPNSTTGGYISNDAMGIPSNAQN
                     PVLAHHFLNYMLDRDAALKNFSWVLYQPPLVDLDPEQLVADEYIFDYLQSTLVFEEDF
                     QMGQLPLQLPPPVDARWIKAWAEVQSG"
     gene            4820..5734
                     /locus_tag="VF097_02960"
     CDS             4820..5734
                     /locus_tag="VF097_02960"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6261790.1"
                     /translation="MTTERGAPGERRWLWPSLAAPGLLWLLIFFVTPVYAILAVAMGR
                     LDPIFGDALPVWNPIQWDPSTFGSVFQAIFGGRLTELFLRTIAFVAIASVTTILIGYP
                     VAYYVSRRAGRWKGLLLLLIVAPFWINYIMRMLAWRNLLDTDGLVNQALMGLGLMGEA
                     RNWLAGNPETVILGLVYGYIPFFILPLYAALDRIDKSVLEAARDLGASPFWAFVRVTL
                     PLSKHGILAGLVIITLPMFGDYYTPNLMGLGRSSVRMIGNEIELFVTSGIGGAEGAAL
                     TLVLMTFVAILMLYYLFSVLRAQREAAG"
     gene            5731..>6024
                     /locus_tag="VF097_02965"
     CDS             5731..>6024
                     /locus_tag="VF097_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261791.1"
                     /translation="MSTETVARPAALPRTGLAARLGPRLRNPWGKPRFLAAWTWIYIV
                     WSIVPVVIAVVFSFNAGRSRSAWQGFSFRWYWEDPDLSVLHDPSLQSAMIQSLK"
BASE COUNT          911 a         2140 c         2059 g          914 t
ORIGIN      
        1 agggggttga ccgccgggag gcccgggtcg aggagctcgg tcagccgggc cttggtccga
       61 tcggagatct gcgcgaaccg cacccccgcc accgcggccc ggtcgatcag gtgcacgcgc
      121 tctcctccgg agtcgtggat cgcggccaga ccgcctgaac gggccctcct ccccgagacg
      181 aacagctcca gcgcgtcggc catctcgtcg agggtctcca cgcggagcgc cccatgggcc
      241 tcgaagacgg cctcgtacgc gccgtcctcg ccggccagcg ccccggaatg ggcctcgatc
      301 aggtcgcgcg tggccgactc gcgtcccacc ttgagcacga cgacgggcac gtcgcgctcc
      361 tccgcccggt ccagcgcctc ccggaacctc tcggggtccc ggacggtctc gatgaacagc
      421 gcggcgacct tcgtcgacgg cagatccagg gcgtacagga ggtagtccga gaccgtggtg
      481 gtcagctcct ggcccgcgga cacggccagg ttgaagcgga ggcctcgatc gttgcggagc
      541 agggccgaga agaccgagcc cgagtggctg aggaacgtga tcggtccggg ctcgagatcc
      601 tcgcgctcgg cgaaggccac cgcccggagg cggcgctcca cgttgacgaa gcccatgcag
      661 ttgcctccgc agaccgcgat cccgctctcg tccacgatcg agcgccatcg ctccagcagg
      721 ggcgggtccc cgggcgactc ctcgtacccg ctggcgaaga tcacgaccga gcgcaccccc
      781 gcctcggccg cgagccggag ctggtcctcg aggcgctcgt tggccacgcc gaggagggca
      841 aggtccacgc cgccggggac gtcggtcagg gaggggacac agggaacccc gagcacctcg
      901 tcgtacttgg gattgacggc gtggacctcg ccctcgaacc ccccgacgag gagctgacgc
      961 atcatctgct cccccaccga ccccgggcgg gcgctggcac ccacgaccgc gaccgcgcgc
     1021 gcctcgagca tggggcgcag gtcgggcatc agcgagcgcg gcgcaagcgc aagcgcgcgc
     1081 ggtcgcgccg gatctcgcgc acgcagttga gcgcggggat ggcggtcacg ccgccgccgg
     1141 cgtgcgtcgc cgacgagcac tggtacaggc cccggatcgg cgttcggtag tccgcgtatc
     1201 cgggcgccgg ccgcatgtgg aacagctgat ccgccgagag ctccccgtgg aagatgttcc
     1261 cgccgatcag cccgagctcc tgctccatgt cgtagggccc gagcacctgc cggtgcagga
     1321 tcgcgccctt gaggttgggg gcgagctccg cgtatccgtc gatcacgcga tccgcgtagg
     1381 cttcgagctc atcccggtgc ggctcggcgg cccactggtg gggcacccac tgcgtgaaga
     1441 gcgacatgat gtgcgtgcct tcgggagcga ggctgcggtc gtacacggtg gggatcacgc
     1501 cgtcggagaa gggccgggcg gcgggacggc cggcccgggc gtcctggaag gcctcttcca
     1561 gatactcgat cgagtgcgcc agttcgatcg cgccgcccat gtggtcgccg ggctcgggct
     1621 tggccgtgaa cctggggagc tcggccaggg ccaggttgat cttcaccgtg ccgctgcggc
     1681 tgttccactg ctcgatgtcc cgcacgaagt cctcgggaag ctccgagcgc tccagctggt
     1741 cgaggaaggt gatccgcgga tgcgtcgagg ccacgacgag gtccgagtgc agctcctcgc
     1801 cgctctcgag cgccacgccc tgggcccgac cgttggtcgt caggatcctg gccacccgtg
     1861 ccccggtgcg gacctcggcc ccgaaggact ccgccgacga ccggatcgca tccgccaccg
     1921 cgcccatccc gccctcgggg aacccccagg tcccgaggcc tcccccggcg tcaccgatct
     1981 cgtgatgcgc gagcacgtag gcggtccccg gcgccgcggg cccggcccag gtcccgatga
     2041 tcccgtcgat cgccagggcg cccttgatct ggggcgactc gaaccagtcg tccagaaggt
     2101 cggagatgct catcgtcatc agccgggtga catcgcccac gccgcgcacc cccagccccc
     2161 ggaagcgcca cgcggtggcc aggagtcgtc ggagctggcc cggcctcatg gagcccaccc
     2221 ggggcggggt cgtcaggagc agcggggcca gcacgtcggc cagtcccgcc agccaggccc
     2281 gccactcggg ataggcgtcg gcgtcgcgct tggagaacct cgccagcgac tcccggtcaa
     2341 gcgactcgtc gggtccggcg aaggtcagcg accccccgtc ggggaaggcg aggaagtacg
     2401 ggcccatcgg gtgcaccgtg tagccgtgcc gccgtaggcg gaggtccttg atgatgcgct
     2461 cgggcatcag gctcatcacg tacgagtacg tggtgacctt cagatccgga gcctcgggcc
     2521 acggggcgtc ggtggccgca gccccgccgg tcttgtgcct ggcctccagg accaccgtct
     2581 tcgccccgag gcgggcgaga tacgcggcgg cggccagccc gttgtggccc ccgccgatca
     2641 cgatcgcgtc atacctcatc ggcagactcc tgactcgccg ttcaatgcac gggagtctac
     2701 cgcgacctcg cgcggcgaag aaacttggct tccgtgcgcg tgaacgtcat cccgccttga
     2761 caacgcggtg gccccctgcc tgaatgaacg ttcataaacc tatggcctca cgtcccaagc
     2821 tgacgagaca gcgccgcatg gagatcctcg aggcggcggt cgagatgatc gtcgagagag
     2881 gactccccga gacccgggtc gccgacgtgg ccgagcgggc cgggacgagc gccgcgctcg
     2941 tcctctacta tttcgaatcg aaggatcgcc tcctcaccga ggcactcagc tacgtcgagg
     3001 accgcttcta cctgcaggcg ttccacgacc tcaccgcgat cgactcggcg cgcgagcggc
     3061 tggccgtctt gatcgctcgc tcgtgccccc cgacgggggg cgacgtccac ggcgactggc
     3121 gcctctggtt cgagctctgg acccgcgcgc tccgcgaccc ggagaccgcg cggaagcggg
     3181 ccgcgctcga ccgccggtgg cgcgccacga tcgccgacgt gatccgcgac gggcagcgag
     3241 cgggcgagtt cggcgcgaac gatccggacg acctcgctct cgagctggcc tcgctgatcg
     3301 acgggctggc gatccaggtc gtcctggagg accccgaggt ggacccggag cggatgcgac
     3361 gcatctgcct cgagaccgct gggcgcgccc tcggggtcga tctctccgtg cacgacctcg
     3421 cgccctcgac ccgggcagcg ccgcggaccg cccgggccca cgtgtcggca tccgaagagt
     3481 agagggggga tcagcatggt gttccatccg cgacagaact ggccgggaag acgggcccgg
     3541 atggacgagc tgtggggacg agggatgcgc aggcgggact tcctgcgctt gtcgggcggc
     3601 tccgccctgg cggccgtgct ggccgcgtgt ggcggtggga acggctcttc gggcgggggc
     3661 ggcggtggca cccagaccga ggtgcaggtg ggaacgcccg agaacccggt cacgcacccg
     3721 atcaccgacg ataacccgat gatcgagtcg ggcctcgagc ccgaggcggg ccctctccgg
     3781 atctacaact gggccgacta catctggccg agagtcctga aggacttcgc cgaggagtac
     3841 aacgtcgagt tcgagctcac gaccttctac aacctggagg aggccctccg gaagctccgc
     3901 accgaggaca tctccttcga catcttcttc cccacggccg agaacatccc gaaggtgatc
     3961 gccggcgggc tcctccagcc gctcaaccac gattacctgc ccaacctgca ggcgaacatc
     4021 tggccccggc tggccgaccc cttctacgac agcggctccc gatacacggt gccctacgcc
     4081 gtgtaccaga cggggatcgg gtggcgcacc gacatgatcg acaccgacat cgagtcgatg
     4141 gagaacccct ggctcgcttt ccaggacccg gcgcaccggg acatcaccgg gctctacgac
     4201 gactatcgcg agacgatggg cgccggtgtg tatatggcgg gcagcaccga catcaacacc
     4261 gatgacccgt cgctcatcga ggagtcgaag cagaacctga tccgggcgat ccaggacgtg
     4321 aacgtagcgt tcacgatcga cggcgcgtac atccagctcc ccgaggccca gctcggcctg
     4381 caccacgctt ggtccgggga cctggcggcc gccccgtact acgcgccgaa gggggtcgac
     4441 cccaccgccc ttcggtacgt gtggccgccg aaggggccga actcgacgac ggggggctac
     4501 atctccaacg acgcgatggg gatcccgagc aacgcgcaga acccggtgct cgcccaccac
     4561 ttcctgaact acatgctcga ccgggacgcg gcgctgaaga acttcagctg ggtcctgtac
     4621 cagccgccgc tcgtcgacct cgacccggag cagctcgtgg ccgacgagta catcttcgac
     4681 tacctgcagt cgacgctcgt gttcgaggag gacttccaga tgggacagct gccgctgcag
     4741 ctcccgccgc cggtcgacgc tcggtggatc aaagcctggg ccgaggtgca gtcgggctga
     4801 ggcccggagg gccgggaggg tgacgacgga gcgcggggcc ccgggggagc ggcgatggct
     4861 gtggccgtcg ctcgccgccc cggggctcct gtggctgctc atcttcttcg tcacccccgt
     4921 ctacgcgatc ctcgcggtgg cgatggggcg tctggacccg atcttcgggg acgccctgcc
     4981 cgtctggaat ccgatccaat gggacccctc gaccttcggc tcggtcttcc aggcgatctt
     5041 cggcgggagg ctgaccgagc tcttcctccg gacgatcgct ttcgtggcga tcgcctccgt
     5101 gaccacgatc ctgatcggct acccggtcgc gtactacgtg tcgaggcgcg cggggcgatg
     5161 gaagggtctg ctcctgctgc tgatcgtggc gcccttctgg atcaactaca tcatgcggat
     5221 gctggcgtgg cggaacctgc tcgacaccga cgggctggtg aaccaggcgc tgatggggct
     5281 ggggctcatg ggcgaggcca ggaactggct ggccgggaac cccgagaccg tgatcctggg
     5341 gctcgtctac ggttacatcc cgttcttcat cctgccgctg tatgcggcgc tggaccgtat
     5401 cgacaagagc gtgctggagg cggctcgcga cctcggggcg agccccttct gggccttcgt
     5461 ccgagtgacg ctcccgctct ccaagcacgg gatcctggcc ggactcgtca tcatcacgct
     5521 tccgatgttc ggcgactact acacgcccaa cctgatgggg ctgggccggt cgtcggtgcg
     5581 catgatcggc aacgagatcg agctgttcgt caccagcggc atcggcgggg cggaaggggc
     5641 cgcgctgacc ctcgtcctca tgacgttcgt ggcgatcctg atgctctact acctcttctc
     5701 cgtgctccgg gcccagaggg aggcggcggg atgagcaccg agaccgtggc ccggcccgcg
     5761 gccctccccc gcaccggtct cgcggccagg ctcggccctc ggctgcgcaa cccgtggggg
     5821 aagccccgct tcctggccgc gtggacctgg atctacatcg tgtggtccat cgtcccggtc
     5881 gtgatcgccg tcgtgttctc cttcaacgcc gggcgctctc gcagcgcgtg gcaggggttc
     5941 tcgttccgct ggtactggga ggaccccgac ctgtccgtcc tgcacgaccc ctcgctgcag
     6001 tccgccatga tccagagcct caag
//
LOCUS       DASUTZ010000065        12325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_406952, whole genome shotgun sequence.
ACCESSION   DASUTZ010000065 DASUTZ010000000
VERSION     DASUTZ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12325
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406952"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..817
                     /gene="whiG"
                     /locus_tag="VF097_02970"
     CDS             29..817
                     /gene="whiG"
                     /locus_tag="VF097_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008745397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HEX6261792.1"
                     /translation="MASDQQAVEQDELTALWKEYKANGSQEIRERLILHYAPLVKFVA
                     GRLAAGLPQNVDQADLTSFGMFGLIDALEKFDLARAVKFETYAIPRIKGAIIDELRAL
                     DWVPRSVRFKARELDKAYQTLEAKLGRSPSDEEVAEHLGIGLDELHEVVSQISFVSVL
                     ALDDVVAGGEDRGESRTLLDTLADAANADPTAELEGQEMRGLLSHAINSLAEREKVVV
                     TLYYFEGLTLAEIGEILGVTESRICQIHTKAVGNLRRSLDDRDA"
     gene            880..1413
                     /locus_tag="VF097_02975"
     CDS             880..1413
                     /locus_tag="VF097_02975"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX6261793.1"
                     /translation="MHGSVVVAVTGILTAIVGSVPGPSAGEAVAAGDYAWPVLGRVLR
                     GFEPAATPYGPGHRGIDIGAERGTRVRAAERGVVAFAGRIAGQRFISILHPGGIRTTY
                     SWLSRVDVAEGQTVLRGAVIGRTGDGHPGSDAPHLHFGAKIDDVYLDPLTLLEPVSLV
                     GIVRLAPLGGAWARSPP"
     gene            1425..2720
                     /locus_tag="VF097_02980"
     CDS             1425..2720
                     /locus_tag="VF097_02980"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX6261794.1"
                     /translation="MRLRAPLVLGLLLSAAWIPLPAGAQDAPWPVAGRTPAHAAVAEG
                     PAPPYRVVWETELEDGPPAAAPVLGEAMLVVLGRSEVAALDPESGDELWSVERAEGTG
                     GAPAVAGDLVLFVEGEGEEAALVAVGAADGEEAWRTEIEGHAPGGVAVAGGRAYVASR
                     RGTVYAVDLEDGSTAWTFPEDPAGRRFESAPAVAGDLLLVTGQITGDRTSFLQALDRE
                     TGEPAWSYRPEGIGLGISSPSEQGSLVLTGTVDGRPRASAFDLAGGASEWTTDIRFPF
                     AGRQMPIVTESDVIIADAAGHLYRLDRSDGELRWTYRVPGFLIGGSPTVAGGSVVVGD
                     LSGQVSAIDLESGLLVWRRSFGDDQVHPVASDGDRLYVAARGGTVAALEHDPDAPLLE
                     EPSPTTLFLDRALLNFGAAFVIVGGALALLSLLWRRRRG"
     gene            2720..3718
                     /locus_tag="VF097_02985"
     CDS             2720..3718
                     /locus_tag="VF097_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261795.1"
                     /translation="MGRKGKRRKRPRGTERSPLRREELVDSAGPEPDGETPAPDPAAT
                     EEQGSDAPRRRGLFSGFSIPSPHPPLLETLGRGVPPAADPRVLAVAFLFVLALWGLVV
                     GLGSEAPPALMAGFWAVPPISVFFDANVVSTIVPGAVASLGLLIVVLALRSLSLTAII
                     RWIAGEQWDPARTEERRGFLATSLSMFAIHSAELGLVLIVFTLAQGLLGGQIGALLAW
                     GLGAYFLGFVPAVVAIEGATAAEGLRRGLRAARLPGARHLTFVMIYLLATLWMSQFAP
                     GGILPPATPSIGIWAYVLAATFVHLAAIGALTYRWIVVRGVVPTGPAPRRRREQAA"
     gene            3855..4874
                     /gene="rpsB"
                     /locus_tag="VF097_02990"
     CDS             3855..4874
                     /gene="rpsB"
                     /locus_tag="VF097_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX6261796.1"
                     /translation="MPVVTRRQLLEAGVHFGHQTRRWNPKMRRYIFGERSGIYIVDLE
                     KTLHGLERAYEFVRDLARRDGTVLFVGTKKQAQDVVAEQAGRVGMPFVNTRWLGGMLT
                     NFRTIFARLQRLTELRRMEESGAFEFLPKKEVLKLRHEKEKLERNMGGIQDMKRVPDA
                     VFVIDTLKEHIAVTESNKLGVPLIAVVDTNCDPDQVDYVIPGNDDAIRSVSLVASVIA
                     DAIEEGKQLAAKDFAERAMGEEEAAPQRPAAPKAAAPEPAPTAAAPAADTAVSESSPA
                     ADAERTGAPAEPEREPATPGGTSSQPGTGNRASARDPEPEGASEAEETTVQPAEGEDE
                     RRGES"
     gene            4904..5509
                     /gene="tsf"
                     /locus_tag="VF097_02995"
     CDS             4904..5509
                     /gene="tsf"
                     /locus_tag="VF097_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX6261797.1"
                     /translation="MEITTAMVKELREATGAGMMDCKRALEEAGGDMERATELLRARG
                     LADAAKRADRVAAEGLVEAYIHMRGSLGVLVELNCETDFVANTDDFRSLARDVAMQIA
                     AIAPRWISRDEVPGDVVDGERKVYEEQARQDGKPDDVIPRIVEGKLEAFYRDHVLLDQ
                     SWVRDDSVTIGELISQAGAKVGEKIEVGRFARFKVGEAAQS"
     gene            5520..6269
                     /gene="pyrH"
                     /locus_tag="VF097_03000"
     CDS             5520..6269
                     /gene="pyrH"
                     /locus_tag="VF097_03000"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX6261798.1"
                     /translation="MTAESGTEATPERPLYGRVLLKLSGDAFALPDREFGISEQSVEL
                     LSAQLADVVSVGTQTAVVVGGGNIFRGRDAPNMDRARADYMGMLATVMNALALQDALE
                     RAGVPTRVQTAIQMAQVAEPYIPRRAIRHLEKGRIVIFAAGIGQPYFSTDTTAAQRAL
                     EIHAEAILKGTQVDGVYDMDPRKNPDAVRFEELEYIEVLQRGLQVMDATAISLCMENG
                     LPIIVFDFRVEGNIKRALTGERIGTLVHAGG"
     gene            6274..6834
                     /gene="frr"
                     /locus_tag="VF097_03005"
     CDS             6274..6834
                     /gene="frr"
                     /locus_tag="VF097_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020630578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX6261799.1"
                     /translation="MAEADVLKEADHKMKRAAEVAQEEFSGVRTGRATPAILNRVMVD
                     YYGTPTPLNQLASMAVPEPRVLTIQPFDPNSVSAIEKAIMQSDLGLTPSNDGHLIRLV
                     FPQLTEERRKELIRVVRERAEEGRVSIRNVRRHAKDELEKMKKDGEISEDDLVRAEKE
                     LQQMTDKHVAEVDELLQRKEQELMEV"
     gene            6834..8684
                     /locus_tag="VF097_03010"
     CDS             6834..8684
                     /locus_tag="VF097_03010"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX6261800.1"
                     /translation="MTEDERREDVDDLFGGLDDFFAPIDDVEWPEDAEREQADEGRGE
                     KAEAEAEPEEAEAAEPNEWTPAIDIPDEEELVGRGEPEEPDAEAAEVAEPPEAPPPDE
                     PETEPALTVDDFFEDEGGEDEGLGEEEEVQPTAEMSGEDWARLRSTLGEDEELEETVE
                     PREGEAAEPRELTIDDLAAGPDQYEELRAEEPADEEAVLAEEAIFEPAEVQEPEGAQV
                     SADEPATAEEVEAAAAHFASGIEPEDVERDLLSDLDEEAPARMAPEPEPVEEPEPEPV
                     EQPPAEDVFEDEEGRKVMRVTPRPSAPLEGEEEPLPLGGTGEPTWQEPAAYAADEDAA
                     AEAGAPPPGRNLGAAVASGVILAAAAVALLAIGKGPFAWLAIAVVLLGQGELYAVLRT
                     RGFQPAAAVGLLAGLFVMVGAYVRGEEGQGEAAMLFGLALATMFSALWYMAAAPKARR
                     GLVANVAATVFGVLYAPFLASFALLLLAQPGNEGRNLLLTVVGLTVLYDVCAYGVGTF
                     WGDRPLAPTISPRKSWEGAIGATFIVVLVALAIVPLFEPFDHPGPAVGLAIVIAIAAP
                     LGDLAESAIKRDLGVKDMSSLLPGHGGVLDRLDAILFTAPAAYYFLRLVL"
     gene            8750..9904
                     /locus_tag="VF097_03015"
     CDS             8750..9904
                     /locus_tag="VF097_03015"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX6261801.1"
                     /translation="MRRVVILGSTGSIGTQALDVIRRNPERFEVVGIAAGSSHELLVG
                     QVREFMPSLVAVSDQDAVTDLRSGLEGLRTVEILTGPDAPVQMARDLDADLVLNAMVG
                     RVGLEPTLTALQTGKTVALANKESLVVGGELVMDLVKGEPERLVPVDSEHSALSQCLR
                     GERREDLRRILITASGGPFRGWTRDELEKASVKEALAHPTWKMGPKITIDSATLMNKG
                     LEIIEAHYLFALEYSRIDVVVHPESIVHSLAEFSDGSTMAQLSNPDMRLPIQLALGFP
                     DRMPTGIEPLDLPSLGRLTFQELDREAFPAVDLCYQAGRAGLTYPAVLNAANEVAVMA
                     FLEGKIPLTGITDVVSRVIDEHDPAGVVSVVYLDRADTWARQRAAELIEQ"
     gene            9905..11104
                     /locus_tag="VF097_03020"
     CDS             9905..11104
                     /locus_tag="VF097_03020"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEX6261802.1"
                     /translation="MRLLRGFLGFLVGLLALNLLIALVVPEGWRGVVLFVEALLLMIL
                     LHEAGHFFTAKWFDIKVEEFFVGFGPRLWSTRRGETEYGIKAIPAGGYVRIAGMNPFQ
                     EPAPEDRDRIFGAKPAWQRAIVLAAGSFTHFVLAILLLASLVAFLGVPVEGPPLVGMV
                     EPRLSGRVSPATSSGMEEGDQIVEVNGEPIDSSEELVEITSGSEGQEMVIVVVRDGER
                     VTLRATPVLDEIQGEQVARLGIRLELPRETEPIHLALWRGIEGTGEIAVESFKALGRV
                     FSPSGLARIGELVTGQAERSVEDPLSIVGAGRLAGQAASTDNYDILLAFLAALNVFVG
                     IVNILPLPPLDGGHLAVLAWEKVSGRKVDMARLIPLTAAVAGLLILLTLALAYLDVAN
                     PVPDPFR"
     gene            11348..>12325
                     /gene="ispG"
                     /locus_tag="VF097_03025"
                     /gene_synonym="gcpE"
     CDS             11348..>12325
                     /gene="ispG"
                     /locus_tag="VF097_03025"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX6261803.1"
                     /translation="MQTERRRSRKVKVGWVEVGGDAPIRVQSMTTTKTADVNATLQQM
                     ASLVAAGCEIVRVAVPHKEDAEALKQIAEHATIPVIADIHFQWRYAMMALEAGIHGLR
                     INPGNIKYQDKVRLIAREAQARGVPIRIGVNAGSLEKDLLAKHGRPTPEALVESAMNE
                     VRILEDEGFHDIKISVKHSDPREMIEAYRLLAERCDYPLHLGVTEAGPMPAGGVKSAV
                     GIGALLAEGIGDTIRVSLTDDPVEEVKVGNWILRSLGLKELGLDLVACPSCGRAEVDV
                     LGLTKQVNEALEREGITEPMRVAVMGCVVNGPGEAREADLGIAAGKGYGQ"
BASE COUNT         1823 a         4241 c         4527 g         1734 t
ORIGIN      
        1 cccgcgagcc tgacgaggag gtcgaccgat cgccagcgac cagcaggccg tcgagcagga
       61 cgagctgacc gcgctctgga aggagtacaa ggcgaacgga tcccaggaga tccgggagcg
      121 cctcatcctc cactacgccc cgctggtcaa gttcgtggcg ggaaggctcg ccgccggcct
      181 cccccagaac gtggaccagg ccgacctcac gtccttcggc atgttcgggc tcatcgatgc
      241 cctggagaag ttcgatctgg cccgcgccgt gaagttcgag acgtacgcga tcccgcgcat
      301 caagggcgcg atcatcgacg agctgcgggc cctggactgg gttcctcggt cggtgcggtt
      361 caaggcccgg gagctcgaca aggcctacca gaccctggag gcgaagctcg gccgctcacc
      421 gtcggacgag gaggtggccg agcacctcgg catcggcctg gacgagctgc acgaggtggt
      481 gagccagatc tcgttcgtct ccgtgctcgc cctcgacgac gtcgtggccg ggggagagga
      541 ccgtggcgag tcgaggacgc tgctggacac gctggccgac gcggcgaacg ccgaccccac
      601 ggcggagctc gagggccagg agatgcgggg cttgctctcc cacgccatca actcgctggc
      661 ggagcgggag aaggtcgtcg tcacgctcta ctacttcgag gggctcacgc tggctgagat
      721 cggggagatc ctgggggtca ccgagtcccg gatctgccag atccacacga aggcggtggg
      781 gaacctccgc cgctcgctgg acgaccggga cgcctgaccg gcgtccgtgt cggaccctcc
      841 tgctagggtc gtcccgtccc cctcgaagga ggtggacgca tgcacgggtc cgtggtcgtg
      901 gccgtcacgg ggatcctcac ggcgatcgtc ggatcggtcc ccggcccctc ggcgggcgag
      961 gcggtcgcgg cgggggacta cgcctggccc gttctgggcc gggtgctgcg gggcttcgag
     1021 ccggcggcga ccccgtacgg accgggccat cgagggatcg acatcggagc cgagcgcggg
     1081 acccgcgtgc gcgccgccga gcggggcgtg gtggcgttcg ccggaagaat cgccggccag
     1141 cgcttcatct ccatcctcca tccaggcggg atccgcacca cctactcgtg gctgtcccgc
     1201 gtggacgtgg cggaaggcca gaccgttctg cgcggggccg tgatcgggcg gaccggcgac
     1261 gggcacccgg ggtccgacgc tccccacctt cacttcggcg ccaagatcga cgacgtctat
     1321 ctcgatcccc tgacgctcct cgagccggtg agcctggtgg ggatcgttcg gctcgccccg
     1381 ctgggagggg cgtgggcccg ctcgcccccc tagaatgggc gaccgtgcgt ttgcgagccc
     1441 ccctcgtcct cggactgctg ctctcggcgg cctggatccc tctgcccgca ggcgcccagg
     1501 acgccccatg gccggtggcc ggccgcacgc ccgcccacgc ggccgtggca gaaggcccgg
     1561 cacccccgta tcgggtggtc tgggagacgg agctcgagga cggacctccc gccgccgccc
     1621 cggtgctcgg cgaggcgatg ctggtcgtcc tgggccgatc cgaggtcgcg gcgctcgatc
     1681 ccgagagcgg cgacgagctg tggtcggtgg agcgtgccga gggcaccgga ggcgcccccg
     1741 ccgtggccgg cgacctcgtc ctcttcgtgg agggggaggg ggaggaggcc gccctggtcg
     1801 ccgtgggcgc cgcggacggc gaggaggcat ggcgaaccga gatcgagggc catgctcctg
     1861 gcggcgtcgc cgtcgccggg gggcgggcgt acgtcgcctc gcgtcgcggg accgtgtacg
     1921 cggtggacct cgaggacggc tcgaccgcgt ggacgttccc cgaggacccc gccgggcgac
     1981 ggttcgagag tgccccggcg gtggccggtg atcttctcct cgtcaccggg cagatcacgg
     2041 gcgaccgaac ctcgttcctc caggccctcg atcgggagac cggcgagccc gcctggagct
     2101 accgccccga gggcatcggc ctcgggatct cctccccctc cgaacagggg agcctcgtgc
     2161 tgacggggac cgtggacgga cggcctcgag ccagcgcctt cgacctggcc ggcggcgcga
     2221 gcgagtggac cacggacatc cgcttcccct tcgcggggcg gcagatgccg atcgtgaccg
     2281 agtccgacgt gatcatcgcc gacgccgcgg gacacctcta ccggctggac cggtcggacg
     2341 gcgagctgcg ctggacctac cgggtcccgg ggttcctcat cggcgggtcc cccacggtcg
     2401 ccgggggctc ggtggtcgtg ggcgacctca gcggccaggt ctcggcgatc gacctggaga
     2461 gcgggctcct cgtctggagg cgctccttcg gcgatgatca ggtccatccc gtcgcctcgg
     2521 acggagaccg tctgtacgtg gccgcccgag gagggacggt ggccgctctc gagcacgacc
     2581 ccgacgcccc cctcctcgag gagccctcgc ccacgaccct cttcctggac cgggcgctgc
     2641 tgaacttcgg ggcggccttc gtcatcgtgg ggggagcgct cgcgctgctg agcttgctgt
     2701 ggaggaggag gaggggctga tgggccgcaa gggtaagcga cggaagcggc cccgcggcac
     2761 ggagcgatct ccgctgcggc gcgaggagct ggtggactcc gccggccccg agccggacgg
     2821 agagaccccc gccccggacc cggcggcgac cgaagagcag ggctccgacg ctccccggcg
     2881 gcgcggcctc ttctcgggct tctcgatccc gtccccccac ccacctctcc tcgagaccct
     2941 cggacgcggc gtccctcccg ccgccgaccc tcgcgtcctg gccgtggcct tcctcttcgt
     3001 gctcgcgctc tggggtctcg tggtagggct ggggagcgag gcccccccgg cgctgatggc
     3061 ggggttctgg gcggtcccgc cgatctcggt cttcttcgac gcgaacgtcg tctcgacgat
     3121 cgtgccgggc gcggtggcct ccctgggact cctcattgtc gtgctcgctc tccgctcgct
     3181 ctcgctgacg gcgatcatcc gctggatcgc cggggagcag tgggaccccg cccggacgga
     3241 ggagcggcgg gggttcctcg ccacgtcgct gtccatgttc gcgatccact ccgccgagct
     3301 cggcttggtg ctgatcgtct tcacgcttgc ccaggggctc ctggggggtc agatcggcgc
     3361 cctcctcgcc tggggcctcg gcgcctactt cctgggattc gttcccgccg tggtcgccat
     3421 cgagggagcc acggcggccg agggcctgcg ccgggggctc cgtgccgcga ggctcccggg
     3481 cgcccgccac ctcacgttcg tcatgatcta cctgctggcc accctctgga tgagccagtt
     3541 cgcccccggg gggatcctcc cgccggccac gccctcgatc gggatctggg cctacgtgct
     3601 cgcggcgacg ttcgtgcact tggccgcgat cggcgcgctg acctaccgct ggatcgtggt
     3661 ccgcggcgtc gtgccgacgg gccccgcccc gcggcgccgg cgcgagcagg ccgcctgacc
     3721 agccgttctg ctaccatcgc ctccggcccc tcagggggta ctacgcacgc ccggggacct
     3781 gctccccggt cccgcgaagc gggttggagc ggggtggagc cgggcggagg accgaaccgg
     3841 aaggaggaag caccatgcct gtggtcacgc gccgccagct gctcgaggcc ggcgtccact
     3901 tcggccacca gacccgcagg tggaacccga agatgcgccg ctacatcttc ggtgagcgga
     3961 gcggcatcta catcgtcgac ctcgagaaga ccctgcacgg cctggagcgc gcctacgagt
     4021 tcgtccgcga cctcgcgcgt cgcgacggca cggtcctctt cgtcgggacg aagaagcagg
     4081 cccaggacgt cgtcgcggag caggccgggc gggtcggcat gccgttcgtc aacacgcggt
     4141 ggctcggcgg gatgctcacg aacttccgca cgatcttcgc gcggctccag cgcctgacgg
     4201 agctgcggcg catggaggag tccggggcct tcgagttcct ccccaagaag gaggtcctga
     4261 agctccgcca cgagaaggag aagctcgagc gcaacatggg cgggatccag gacatgaagc
     4321 gggtgcccga cgccgtgttc gtgatcgaca cgctgaagga gcacatcgcg gtcacggagt
     4381 cgaacaagct cggcgtcccc ctgatcgcgg tcgtggacac gaactgcgat cccgaccagg
     4441 tcgactacgt gatccccggg aacgacgacg cgatccggtc cgtctcgctc gttgcgagcg
     4501 tgatcgccga tgcgatcgag gagggaaagc agctcgcggc gaaggacttc gccgagcggg
     4561 ccatggggga ggaggaggcc gcgccccagc ggccggccgc accgaaggcc gcggcgcccg
     4621 agccggcgcc caccgccgcc gctcccgccg ccgacaccgc ggtctcggag tcgagccccg
     4681 cggccgatgc cgagcggacc ggcgcccccg ccgagcccga gcgcgagccg gccacgcccg
     4741 gagggacctc gtcccagccc gggacgggga accgggcctc ggcacgagat cccgagccgg
     4801 agggcgcctc cgaggccgag gagacgaccg tgcagccggc cgagggagag gacgagcggc
     4861 gaggggaatc ctgaccctca ctatgggaga atcgccagga ccgatggaga tcacgaccgc
     4921 gatggtgaag gagctccggg aggccaccgg ggccggcatg atggactgca agcgcgccct
     4981 cgaggaggcc gggggcgaca tggagcgcgc caccgagctg ctccgggccc gggggctcgc
     5041 ggacgcggcg aagcgcgccg atcgcgtcgc ggccgagggg ctcgtcgagg cctacatcca
     5101 catgcggggc agcctcggcg tgctcgtgga gctcaactgc gagaccgact tcgtggccaa
     5161 caccgacgac ttccggtccc tggctcgcga cgtggccatg cagatcgcgg cgatcgcccc
     5221 gcgctggatc tcgcgcgatg aggtaccggg cgacgtcgtg gacggtgagc ggaaggtcta
     5281 cgaggagcag gctcgccagg atggcaagcc ggacgacgtg atcccccgca tcgtggaggg
     5341 gaagctcgag gccttctacc gggaccacgt gctcctcgac cagtcgtggg tgcgtgacga
     5401 ttcggtcacg atcggggagt tgatctccca ggccggcgcg aaggtcggag agaagatcga
     5461 ggtcggccgg ttcgcacgct tcaaggtggg cgaggcggcc cagagctgag ggagggccat
     5521 tgacggccga gtcggggacg gaggcgacgc ccgagcggcc gctgtacggg cgggtgctcc
     5581 tgaagctctc cggcgacgcc ttcgcccttc ccgaccgcga gttcggcatc tccgagcagt
     5641 cggtcgagct tctgtccgcc cagctggcgg acgtcgtctc ggtgggcacc cagaccgccg
     5701 tggtggtggg gggcggcaac atcttccgcg gccgggacgc gcccaacatg gaccgggcgc
     5761 gggccgacta catggggatg ctggccacgg tgatgaacgc cctggctctc caggacgcgc
     5821 tggagcgcgc gggcgtcccc accagggtgc agaccgcgat ccagatggca caggtcgccg
     5881 agccctacat cccccggcgc gcgatccgcc acctggagaa ggggcggatc gtgatcttcg
     5941 ccgcggggat cggccagccc tacttctcca ccgacacgac cgcggcccag cgggccctcg
     6001 agatccacgc cgaggcgatc ctcaagggga cgcaggtcga cggggtctac gacatggacc
     6061 cgcggaagaa ccccgacgcc gtgcgcttcg aggagctcga gtacatcgag gtcctccagc
     6121 gggggctcca ggtgatggac gccacggcca tctccttgtg catggagaac ggcttgccca
     6181 tcatcgtctt cgacttcagg gtggagggga acatcaagcg cgccctgacc ggcgagcgga
     6241 tcgggaccct ggtgcacgcg ggaggataga cggatggcgg aagcggacgt cctgaaggaa
     6301 gccgaccaca agatgaagcg ggcggcggag gtggctcagg aggagttctc tggggtgcgc
     6361 acgggacggg ccacgccggc gatcctcaac cgcgtgatgg tcgactacta cggcacgcca
     6421 accccgctga atcagctcgc ctcgatggcg gtgcccgagc cgcgggtcct cacgatccag
     6481 ccgttcgatc cgaactcggt gtcggcgatc gagaaggcga tcatgcagag cgacctcggg
     6541 ctcaccccct cgaacgacgg gcacttgatc cggctcgtct tcccccagct cacggaggag
     6601 cgccgcaagg agctgatccg cgtcgtgcgg gagcgcgccg aggaggggcg cgtctcgatc
     6661 cgcaacgtcc gccgccacgc caaggacgag ctggagaaga tgaagaagga cggcgagatc
     6721 tccgaggacg acctcgtccg ggccgagaag gagctccagc agatgacgga taagcacgtc
     6781 gccgaggtgg acgagctcct gcagcggaag gagcaggagc tcatggaggt ctgatgaccg
     6841 aggacgagag acgagaggac gtcgacgacc tgttcggcgg ccttgacgac ttcttcgcgc
     6901 cgatcgacga cgtggagtgg cccgaggatg ccgagcgcga gcaggcggac gagggccggg
     6961 gcgaaaaggc ggaggccgag gccgagcccg aggaggccga ggcggcggag ccgaacgagt
     7021 ggacccccgc gatcgacatc ccggacgagg aggagctcgt gggccggggc gagcccgagg
     7081 agcccgacgc cgaggccgcc gaggtcgcgg aaccccctga ggcgccaccg cccgacgagc
     7141 ccgagaccga gcccgccctg accgtggacg acttcttcga ggatgaaggg ggcgaggacg
     7201 agggtctcgg ggaggaggag gaggtccagc ccaccgccga gatgtccggg gaggactggg
     7261 cccggctccg ttccaccctg ggggaggacg aggagctcga ggagaccgtc gagcctcgag
     7321 agggggaggc ggccgagccg cgcgagctga cgatcgacga cctggccgcc ggccccgacc
     7381 agtacgaaga gctgcgagcc gaggagccgg cggatgagga agccgtgctg gccgaggagg
     7441 cgatcttcga gccggccgag gtgcaggagc cggagggcgc gcaggtctcc gccgacgagc
     7501 ccgccacggc ggaggaggtg gaggccgccg ccgcgcactt cgcatccggg atcgagccgg
     7561 aggacgtcga gcgcgatctg ctctccgacc tggacgagga ggcgccagcc cggatggcgc
     7621 ccgagcccga gcccgtcgag gagcccgagc ccgagcccgt cgagcagccc cccgccgagg
     7681 acgtcttcga ggacgaggag gggcggaagg tgatgcgggt gacgcctcgt ccgtccgctc
     7741 cgctggaggg agaggaggag cccctcccgc tgggcggcac gggggagccg acgtggcagg
     7801 agcctgcggc ttacgccgcc gacgaggacg ccgcggcgga ggccggcgcc ccgccccccg
     7861 gacgcaacct gggggccgcc gtggcgtccg gcgtgatcct ggccgcggcc gccgtcgcgc
     7921 tcctggcgat cggcaagggc cccttcgcct ggctggccat cgccgtcgtc ctcctggggc
     7981 agggtgagct gtacgccgtc ctgaggaccc gtgggttcca gccggccgcc gcggtcggcc
     8041 tgctcgccgg cctcttcgtc atggtcggag cgtacgtgag gggcgaggag ggccagggcg
     8101 aggccgcgat gctcttcggc ctggcgctgg ccacgatgtt ctcggcactc tggtacatgg
     8161 ccgctgcacc gaaggcccgg cgaggcctgg tggccaacgt ggccgcgacg gtcttcggcg
     8221 tgctgtacgc gcccttcctc gcctccttcg ccctcctcct gctcgcgcaa cccggcaatg
     8281 aggggcggaa cctgctgctc accgtggtcg ggctgacggt gctctacgac gtctgcgcct
     8341 acggggtggg caccttctgg ggcgatcgcc cgctggcgcc gacgatcagc ccgaggaagt
     8401 cgtgggaggg agcgatcggc gccacgttca tcgtggtgct cgtggccctg gcgatcgtgc
     8461 cgctgttcga gcccttcgac catccgggcc cggcggtggg cctggccatc gtgatcgcga
     8521 tcgccgcgcc gctcggcgac ttggccgagt cggcgatcaa gcgcgacctc ggcgtcaagg
     8581 acatgagctc cctgctcccg ggccacgggg gcgtgctgga tcgactcgat gccatcctct
     8641 tcaccgctcc cgcggcgtac tacttcctga gactcgtcct gtagcccctc cgggggatgc
     8701 ggacgacctc gatgtaattg ccaacgcgag cgggtaccct gggagcggta tgcggcgagt
     8761 cgtcatcctc ggttcgacgg gctcgatcgg gacccaggcg ctggacgtga tccggcgcaa
     8821 tccggagcga ttcgaggttg tcggcatcgc ggcgggctcc agccacgagc tgctggtggg
     8881 tcaggtccgc gagttcatgc cctcgctggt ggcggtgagc gaccaggacg cggtgacgga
     8941 cctgcggtcg gggctggagg gcctgcgcac ggtcgagatc ctgaccggcc ccgacgcgcc
     9001 ggttcagatg gctcgggacc tcgacgccga cctggtcctc aacgcgatgg tgggtcgggt
     9061 cggcctggag ccgacgctga ccgcgctcca gaccgggaag acggtggcgc tcgccaacaa
     9121 ggagagcctc gtcgtcgggg gcgagctcgt catggacctg gtgaagggcg agcccgagcg
     9181 gctcgtcccg gtggactcgg agcactctgc gctctcccag tgcctgcggg gcgagcgacg
     9241 ggaggacctc cgtcgcatcc tgatcacggc ctcgggcggg cccttccggg ggtggacccg
     9301 ggacgagctc gagaaggcct cggtgaagga ggccctggcc cacccgacct ggaagatggg
     9361 tcccaagatc acgatcgact cggccacgct gatgaacaag ggcctcgaga tcatcgaggc
     9421 tcactatctc ttcgccctcg agtactcgag gatcgacgtg gtcgtgcacc cggagagcat
     9481 cgtccacagc ctcgccgagt tcagcgacgg cagcacgatg gctcagctct cgaaccccga
     9541 catgcgcctg cccatccagc tcgcgctggg gttccccgac cgcatgccga ccggcatcga
     9601 gccgctcgac ctcccctcgc tggggcgcct caccttccag gagctcgatc gcgaggcgtt
     9661 ccccgccgta gacctctgct atcaggcggg gcgggcaggc ctgacgtatc cggcggtgct
     9721 caacgccgcg aacgaggtcg cggtcatggc cttcctcgag gggaagatcc cgctgaccgg
     9781 catcaccgac gtcgtgtcgc gggtcatcga cgagcacgat cccgcagggg tcgtctcggt
     9841 cgtgtacctc gaccgcgcgg acacctgggc gcgccagcgc gcggccgagc tcatcgagca
     9901 gtagatgcgc ctgctccgcg gcttcctcgg gttcctcgtg ggcctgctcg ccctcaacct
     9961 cctgatcgcc ctggtcgtgc ccgagggatg gcgcggcgtc gtgctgttcg tggaggccct
    10021 cctcctgatg atcctgctcc acgaggccgg ccacttcttc accgcgaagt ggttcgacat
    10081 caaggtcgag gagttcttcg tcgggttcgg ccccaggctc tggtccacca ggcgagggga
    10141 gacggagtac gggatcaagg ccatccccgc aggcggctac gtgcggatcg cgggcatgaa
    10201 cccgttccag gagcccgcgc ccgaggaccg cgatcggatc ttcggagcca agccggcgtg
    10261 gcagcgggcc atcgtcctgg cggcggggtc cttcacccac ttcgtcctgg cgatcctcct
    10321 gctggcctcg ctcgtggcct tcctcggggt gccggtggag ggcccgccgc tcgtcgggat
    10381 ggtggagccg aggctctcgg gccgggtctc cccggcgacg agctcgggga tggaggaggg
    10441 cgatcagatc gtggaggtga acggagagcc gatcgactcc agcgaggagc tcgtcgagat
    10501 cacctcgggg agcgagggcc aggagatggt gatcgtggtt gtgcgggacg gtgagcgggt
    10561 cacgctccgg gccacgcccg tcctcgacga gatccagggg gagcaggtgg ctcgcctcgg
    10621 gatccgcctc gagctccccc gcgagaccga gcccatccac ctggctctgt ggcgcggcat
    10681 cgaggggacc ggggagatcg cggtcgagtc cttcaaagcc ctggggcggg tgttcagccc
    10741 gagcggcttg gccaggatcg gggagctggt caccggccag gccgagcgct ccgtcgagga
    10801 ccccctcagc atcgtcggag cggggcgact cgcgggccag gccgcctcca ccgacaacta
    10861 cgacatcctc ctggccttcc tggccgcgct caacgtgttc gtggggatcg tcaacatcct
    10921 cccgctgccg ccgctcgacg gcggccacct cgccgtgctg gcgtgggaga aggtctctgg
    10981 gcgcaaggtc gacatggcca ggctgatccc gctcaccgcc gctgtggccg gcctgctgat
    11041 cctcctcacc ttggcgctgg cctacctgga cgtggccaac ccggtgcccg acccgttccg
    11101 gtaaggctcg cccccagacc gaccggcctt aaggcacccg aaggggccac ccgacaatcg
    11161 gtccggtcgg cggttgggtg ccgccgccca tggaggtcgg gatcatgagg agggtgatca
    11221 ccaacctcag gatcctggtc gtcggctata ccgagatcgc gggaggcgac atcctggcag
    11281 ctcggtccct gggctgaacg aagcccctcc gggccgggtt cggaggggcc gtcggtacac
    11341 tggagccgtg cagaccgaac gccgaaggtc ccggaaggtc aaggtgggct gggtcgaggt
    11401 cggcggcgac gccccgatcc gcgtccagtc gatgacgacc acgaagaccg cggacgtcaa
    11461 cgccaccctc cagcagatgg cgtcgctcgt cgcagcgggg tgcgagatcg tgcgcgtcgc
    11521 cgtgccccac aaggaagacg ccgaggcgct gaagcagatc gccgagcacg ccacgatccc
    11581 cgtcatcgcc gacatccact tccagtggcg ctacgcgatg atggccctcg aggccgggat
    11641 ccacggcctg cgcatcaacc cgggcaacat caagtaccag gacaaggtcc ggctgatcgc
    11701 ccgcgaggct caggcccgcg gcgtcccgat ccggatcggg gtcaacgccg ggtcgctgga
    11761 gaaggacctc ctggccaagc acggacgccc cacccccgag gccctcgtcg agtcggccat
    11821 gaacgaggtc cgcatcctcg aggacgaggg cttccacgac atcaagatct ccgtgaagca
    11881 ctcggacccg agggagatga tcgaggccta tcggctgctc gccgagcggt gcgactaccc
    11941 gctgcacctg ggggtgaccg aggcgggccc catgcccgcg ggcggggtga agtcggccgt
    12001 cggcatcggg gccctgctgg ccgagggcat cggcgacacg atccgcgtct ccctcaccga
    12061 cgacccggtc gaggaggtca aggtcgggaa ctggatcctc cgctcgctgg ggctgaagga
    12121 gctcgggctc gacctcgtgg cctgtccctc gtgcggccgg gcggaggtgg acgtcctcgg
    12181 cctcacgaag caggtcaacg aggcgctgga gcgcgagggc atcaccgagc cgatgcgggt
    12241 ggccgtgatg ggctgcgtcg tgaacgggcc cggcgaggct cgcgaggccg acctggggat
    12301 cgcggccggg aagggctacg gccag
//
LOCUS       DASUTZ010000066        10814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1833261, whole genome shotgun sequence.
ACCESSION   DASUTZ010000066 DASUTZ010000000
VERSION     DASUTZ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10814
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1833261"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1349)
                     /locus_tag="VF097_03030"
     CDS             complement(<1..1349)
                     /locus_tag="VF097_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX6261804.1"
                     /translation="MGTQSPAPHVADTHDLIRVHGARENNLKDVSIEIPKRRLTVFTG
                     VSGSGKSSLVFDTIAAESQRLINETYSAFVQGFMPTLARPDVDLLEGLTTAIIVDQER
                     MGANARSTVGTATDANALLRILFSRLGQPHVGPATAFSFNVPTRKASGVMTSDTGERR
                     VARGVVYLGGMCPRCEGMGSVTDIDVAQLYDETKSLNEGAITVPGYKAGGWSVKLFAE
                     SGFLDPDKPIRDYTRKELHDFLYREATKVKLGGVNLTYEGLVPRIQQSFLSKDREAMQ
                     PHIRAFVDRAVTYITCPECDGTRLNEAARSSKIAGLNIAEVCAMQISDLAGWVSDLDE
                     PSVAPLLATLRHTLDSFVEIGLGYLSLDRPSGTLSGGEAQRTKMIRHLGSALTDVTYV
                     FDEPTIGLHPHDVQRMNDLLLRLRDNGNTVLVVEHEPETIAIADHVVDLGPGAGTSGG
                     "
     gene            1520..3460
                     /locus_tag="VF097_03035"
     CDS             1520..3460
                     /locus_tag="VF097_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX6261805.1"
                     /translation="MFREKFWLSLALTLPVVVWSAHIQELLKYRAPEFPGSDWIPPVL
                     GTAVFLYGGLVFLQGAVRELRARLPGMMVLIALAITVAFVFSWVVQLGLLDAEALWWE
                     LATLVTIMLLGHWIEMRSISQAQGALQELAKLLPDTATRITDAGEETVSVTELRDGDV
                     VLVRPGESIPADGVVRRGESDLNEAMITGESRPVKKEEDDKVIAGTINGEGSLRVEVT
                     GTGEETKLSGIMRLVADAQTSKSRAQHLADRAAQILTGVAILAGVLTLIVWQLLGAAI
                     DFSIVRTVTVLVIACPHALGLAVPLVVAISTTLGARNGLLVRDRRGLEEARNVDIVIF
                     DKTGTLTLGEFRVVDMAAADGSSEEDMLRVAASVESESEHPIARGILRTAEDRGLEVP
                     PAEGFRALTGRGVAASVDGTEYHLGGPALLQAEDARVPEHLEKAASAAADRGQAAIYL
                     LGDGEAMAVFAVADAIREESREAIRALHEAGIEVAMLTGDAQAVADAVAEELGIDRVF
                     AEVRPEDKASKVKELQAQGKRVAMVGDGVNDAPALATAEVGIAIGAGTDVAVEAGDIV
                     LVRSDPRDIPRIVTLSRGTYRKMLQNLWWAAGYNIFAIPLAAGALAAWGILLTPALGA
                     VLMSASTVIVAINAQLLRRARL"
     gene            3586..4044
                     /locus_tag="VF097_03040"
     CDS             3586..4044
                     /locus_tag="VF097_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261806.1"
                     /translation="MRRLASLLPYIPVLVGVGWLATRFRIVLGVFLIAHGLVHVMFVV
                     PEPAREEGGLEWPFHLDRSWALSGLGSGPARAIGILLMIATIAAFAVAGIALLADLGW
                     WSGAAIAGAAASTALLGLYFHPMLVLGLAINAFVLTVAGLGWPTLSYSEA"
     gene            complement(4144..4493)
                     /locus_tag="VF097_03045"
                     /pseudo
     CDS             complement(4144..4493)
                     /locus_tag="VF097_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003953106.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4872..5960
                     /locus_tag="VF097_03050"
     CDS             4872..5960
                     /locus_tag="VF097_03050"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX6261807.1"
                     /translation="MIIDEPVRGRYAYLEHPWLFALPPHEQMRPFIEGDVPRPPIHHL
                     TGLIPVEAGPTSSVWSMPASPWWRSAAGIFPGGALAIVADAALSGAIYGGLPKGTALA
                     TSELTMNFVRPATSESGELIATGSLIHSGSRQGLSDGRIRDASGRLLAHATSRCVLRR
                     LPFDPPDPPATLPPYKQSDHDTPDPYLRPPEGVVIPQEEWDASPGLDLVRSWSGGARG
                     TPPVCRLSGWRLREAEEGATTWAMPASEWFCTGFGTFYGGIITLFLDGAINTAVTTTL
                     PPRASFGTLDLKVNFLRPVSPDGRDLVVRAKVAHRGRTVAVTTAEMHDADGKRVAMAT
                     SSAMILPGVPWNAAKPPETLDEAPDEQG"
     gene            6002..6295
                     /locus_tag="VF097_03055"
     CDS             6002..6295
                     /locus_tag="VF097_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013415177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX6261808.1"
                     /translation="MAARVSDVYRAIADPSRRAILDELVDRDGQTLFEVCARLAMRHG
                     LTSSRQAVSQHLAVLEEAGLVRTKREGRYTFHFLDTRPLGEVIERWPIERRTR"
     gene            6292..6678
                     /locus_tag="VF097_03060"
     CDS             6292..6678
                     /locus_tag="VF097_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019523683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6261809.1"
                     /translation="MRINLTSVYVDDQEKALRFYTEVLGFRKKSDMPLGEGARWLTVV
                     SPEDPDGPELLLEPSEHPAATPFKEALVSDGIPFTSFAVKDVRAEAERLKGLGVRFTQ
                     EPVEMGPVTMAVFDDTCGNLIQIAAT"
     gene            complement(6675..7052)
                     /locus_tag="VF097_03065"
     CDS             complement(6675..7052)
                     /locus_tag="VF097_03065"
                     /inference="COORDINATES: protein motif:HMM:NF025852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6261810.1"
                     /translation="MEPGRAIIADALASLELRPSHPVIYDREIGLRLLYQDPASGAEH
                     YLVRYPAGLRTRLHRHTASHTVVVLEGRLRVNEVVVGPGAYCHFPAGEAMRHAPADDA
                     GCLFVTIFHGPFDVEPLEDEAKS"
     gene            7163..7675
                     /locus_tag="VF097_03070"
     CDS             7163..7675
                     /locus_tag="VF097_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261811.1"
                     /translation="MRRLIVSVATLGAIALASPASAVPPQHFSVDPPPNIVEDNGEAC
                     GFPVRWTIDLEVQGTNFFDQDGVIVRQQAHVQEDNTIENLDTGLTLREGPDSFMQTIH
                     FENGVPTHFVATGLGLNVFGEQLKDVGRVAWLPGTNEIIFSAGPHEARELLQFGTFED
                     VLAFFCDVLA"
     gene            7747..9249
                     /locus_tag="VF097_03075"
     CDS             7747..9249
                     /locus_tag="VF097_03075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6261812.1"
                     /translation="MRILVWHGWILEGSGSNVYTARIVEAWRARGHDVLLVAQERHPE
                     RFAFLDAWADGNLTEPHPTGVEPSLGRATLLRPDIGDLLPVFVWDAYEGFRVKTFVDL
                     SDEELEGYLDRNVAALREAAEAHGTDVAIAGHAIPGANVARRALGSIPFAAKIHGSDV
                     EYAMRGQRRYVELAREGLEGAVVIAGASRDVLDRLIELVPSVAGRTKVVPPGVEVERF
                     HPMPRAEALRGAASLLERDPALARGRPETAASDVAEALRRRDGDALDRLANTYEQTSP
                     DRDAPRRLRALAGYDGPLVGYLGKLIPQKGVHDLLAALALLPSRPRALVVGFGTFREW
                     LEALVAALDAGDPVAAAFLEERMPLGLSPGEVAAAAGLAERVVFTGRLDHRYAGPAAA
                     ALDVLVVPSILEEAFGMVALEGAAAGALPLVARHSGLAEVADALETEIGRPGLLSYEP
                     GLSAPRALGRALEGLLALPDRRELAAHLSSHVRERWSWGRTADLLLEPLR"
     gene            complement(9246..10292)
                     /locus_tag="VF097_03080"
     CDS             complement(9246..10292)
                     /locus_tag="VF097_03080"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX6261813.1"
                     /translation="MLERVRAWTYARQRLGRPARGALEALRAVVAVYATHPTAPLALR
                     ARTRSLTGTAYRRLDRERRGLRIPGMRGTVFLVPREAAARIFTAVRPPRSKVVARLKR
                     HDMSEEEYEAIARRVVAVAGEPARAEDLREATGLEGPVLATILRGLRLEGRLLALAGE
                     PLTSSPHRYVATEAWAEDGLDAGDREEALAWLAGAYLEAYGPARVEDFAWWAGVGKRA
                     SAAAVAEHDTIDAGEGLLLPARHEAAFERTRRLKGTLALLPRWDAYTMGHAPDGRARF
                     VHPDVQQRVYEPIGVGLPGDGNPVVLVDGEVVATWTYSLKDGAEVQPFDTLGPRTRGR
                     IEGEVDAIAALLAT"
     gene            complement(10293..10565)
                     /locus_tag="VF097_03085"
     CDS             complement(10293..10565)
                     /locus_tag="VF097_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019158379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HEX6261814.1"
                     /translation="MNETALGVVAASWGVVMALSPVLQIRRILTYRSSRDVSIGYLTV
                     IAIGFALWIVYGLAIENVALIVPNTLAFIVGVIVIGVALRFRSPED"
BASE COUNT         1554 a         3799 c         3849 g         1612 t
ORIGIN      
        1 ccgcccgacg tcccggcgcc ggggccgagg tcgacgacgt gatcggcgat cgcgatcgtc
       61 tcgggctcgt gctccaccac gagcaccgtg ttgccgttgt cccgcaggcg cagcaggaga
      121 tcgttcatcc gctggacgtc atgagggtgc aggccgatgg tcggctcgtc gaagacgtag
      181 gtgacgtcgg tgagggcgga gccgaggtgg cggatcatct tcgtgcgctg cgcctcaccg
      241 cccgagaggg tgcccgacgg ccggtcgagc gagaggtagc ccagcccgat ctccacgaac
      301 gagtcgaggg tgtgccgcag cgtggcgagc agcggcgcca ccgacggctc gtcgaggtcg
      361 ctgacccatc ccgccaggtc gctgatctgc atcgcgcaga cctcggcgat gttgagcccc
      421 gcgatcttcg aggagcgggc agcctcgttg agtcgcgtgc cgtcgcactc ggggcacgtg
      481 atgtaggtga ccgcccggtc cacgaacgcc cggatgtgcg gctgcatggc ctccctgtcc
      541 tttgagagga acgactgctg gatccgcggg accagtccct cgtaggtgag gttgacgccg
      601 cccagcttga ccttggtcgc ttcccggtac aggaagtcgt gcagctcctt cctcgtgtag
      661 tcccggatcg gtttgtcggg gtcgaggaat cccgactcgg cgaagagctt taccgaccac
      721 ccgccggcct tgtagccggg gacggtgatg gcgccctcgt tgagcgactt ggtctcgtcg
      781 tagagctggg ccacgtcgat gtccgtcacg ctgcccatgc cctcgcagcg ggggcacatg
      841 ccgccgaggt acacgacgcc gcgggcgacc ctgcgctcgc cggtgtcgga ggtcatcacg
      901 ccgctcgcct tcctcgtggg gacgttgaag gagaaggcgg tggccggccc gacgtggggc
      961 tgccccaggc ggctgaagag gatgcgcagc agcgcgttgg cgtccgtcgc ggtaccgacc
     1021 gtggagcgcg cgttggcgcc catccgctcc tggtcgacga tgatggcggt ggtgagcccc
     1081 tccagcaggt cgacgtcggg ccgcgccagc gtcggcatga agccctggac gaaggcgctg
     1141 tacgtctcgt tgatcagcct ctgagattcg gcggcgatcg tgtcgaagac cagggagctc
     1201 ttgcccgagc cggagacgcc cgtgaacacc gtcagccggc gcttggggat ctcgatgctg
     1261 acgtccttga ggttgttctc gcgcgcgccg tgcacgcgga tcagatcgtg ggtgtcggca
     1321 acgtgcggcg cgggggactg cgttcccatg cgttcagagt acggtgtcag gcgaggacaa
     1381 tcggatcctc accatgagcg ataccacgtc acaccagcgg ggtcacggag ccgcccctaa
     1441 cgccgcgacg ccggcgcggg acggggaggc gtcggcggag cacacgggcc acgataagca
     1501 cgagggccac agcccggaga tgttccggga gaagttctgg ctctcccttg cgctcaccct
     1561 cccggtggtc gtctggtcgg cacacatcca ggagctcttg aaataccggg cgccggagtt
     1621 ccccggctcg gactggatcc caccggtgct ggggacggcg gtattcctct atggcggtct
     1681 ggtcttcctc cagggggccg tgcgcgagct ccgggctcgc ctgccgggga tgatggtcct
     1741 catcgccctg gccatcaccg tggccttcgt cttctcctgg gtggtgcagc tggggctcct
     1801 cgacgccgag gccctctggt gggagctggc caccctggtg accatcatgc tgctggggca
     1861 ctggatcgag atgcgctcga tctcccaggc gcagggtgcg ctccaggagc tggcgaagct
     1921 ccttcccgac accgccaccc gcatcaccga cgccggggaa gagaccgttt ccgtcacgga
     1981 gcttagggat ggggacgtcg tgctggttcg tcccggcgag agcatcccgg cggacggcgt
     2041 ggtgcgaagg ggcgagagcg acctcaacga ggccatgatc accggcgagt ccaggccggt
     2101 gaagaaggag gaagacgaca aggtcatcgc ggggacgatc aacggcgagg gctccctccg
     2161 ggtggaggtc accgggaccg gagaggagac gaagctctcg gggatcatgc gcctcgtggc
     2221 cgacgcccag acatccaaat ctcgggccca gcacctggcc gatcgggcgg cccagatcct
     2281 cacgggggtg gccatcctag cgggggtcct caccctgatc gtgtggcagc tcctcggcgc
     2341 ggcgatcgac ttctccatcg tcaggacggt gacggtcctg gtcatcgcct gcccccacgc
     2401 cctggggctc gccgtccccc tggtcgtggc catctccacc accctggggg cgcggaacgg
     2461 cctcctggtg cgcgatcgcc gagggctgga ggaagcccgg aacgtggaca tcgtgatctt
     2521 cgacaagacc gggaccctca ccctgggcga gttccgggtc gtggacatgg ccgcggccga
     2581 cgggagctcg gaggaggaca tgctccgggt tgccgcctca gtggagtccg agtccgagca
     2641 tcccatcgcc cgggggatct tgaggacggc ggaggaccgg gggctggagg tgcccccggc
     2701 ggaggggttc cgggcgctga ccgggagagg cgtcgccgcc tccgtcgacg ggaccgagta
     2761 ccacctcgga ggaccggcgc tcctccaggc ggaggacgct cgggtgcccg aacacctgga
     2821 gaaggcggcc tccgcagcgg cggaccgcgg ccaggctgcg atctacctgc tcggtgacgg
     2881 cgaggccatg gcggtcttcg ccgtggccga cgcgatccgg gaggagagcc gcgaggcgat
     2941 ccgcgccctc cacgaagccg gcatcgaggt ggccatgctc accggcgacg cgcaggcggt
     3001 ggcggacgcg gtcgcggagg agctcgggat cgatcgggtg ttcgcggagg tgcgtcccga
     3061 ggacaaggcg tccaaggtga aggagctcca ggcgcagggc aagcgggtgg ccatggtggg
     3121 ggacggggtg aacgatgcgc cggccctcgc cactgccgag gtcggcatcg cgatcggggc
     3181 ggggacggac gtggcggtgg aggccgggga catcgtgctg gtgcgctcgg accctcgcga
     3241 catcccccgg atcgtcaccc tctccagggg aacctaccgg aagatgctcc agaatctctg
     3301 gtgggcggcc ggctacaaca tctttgccat ccccctggcg gcaggggcgc ttgccgcgtg
     3361 ggggatcctg ctgacgcccg ccctgggtgc cgtgctcatg tccgcgagca ccgtgatcgt
     3421 ggcgatcaac gcacagctct tgaggagagc tcgcctttga ccgccttccc cgtgtccgtc
     3481 accgggccca tgggacgcga gtcctcccgg ccgggggccg aacgcctccc ctcctgaggg
     3541 gtggccatcc acgagcattg gggcgagcag agaggagtga tcgacatgcg acgcctcgcg
     3601 tccctgctgc cgtacatccc ggtgctcgtc ggcgtcggct ggctcgccac ccggttccgc
     3661 atcgtcctcg gcgtcttcct gatcgcgcac ggcttggtcc acgtcatgtt cgtcgtcccc
     3721 gagccggccc gggaggaggg cggcctcgag tggccgttcc acctcgaccg gtcgtgggcg
     3781 ctgtcgggcc tcggctcggg gcccgctcgg gcgatcggga tcctgctcat gatcgccacg
     3841 atcgccgcgt tcgccgtggc cgggatcgcc ctgctggccg atctcggctg gtggagcgga
     3901 gccgcgatcg ccggcgccgc ggcctcgacc gcgctgctgg ggctgtactt ccaccccatg
     3961 ctcgtgctcg gactggccat caacgccttc gtcctgacgg tcgccgggct cgggtggccc
     4021 accctcagtt actcggaggc atgagcgcga ggagctccct gacggccgcc gagaaccgtc
     4081 ccccgccgac gcctatcgga tcctcgtggc cgtggtcgag tagccgccgc cgcccgggta
     4141 cacctaggcg ccggccagcg tgttgccgtc cgggctgaaa gaccctggta gaaggcggga
     4201 gagccccgct cgccgcccca gatcgtgagc tgctcgccct cgaggtcgta cacgtagtcg
     4261 aacgtgttgc cgaggttgtc gtagtagcgg gaccgcacgt cctcgcccgg ctcctcggcg
     4321 ccgaagggcc gttcgcggcc gatgatctcg atccccgtcg tgcgctggcc gaactgcacg
     4381 agatcgaccc gctgcagcag gaagtgtcgc ccctcgagcc actcgtagct gacctccccc
     4441 tcggcctcgc ccgcgaccct ccacgtgccc accagccgat cgaggccctg catcgccgga
     4501 tcgccctcca ccgccaaccg gtccttctcc gtcttggaca cgatctcctc ctcgttcgtg
     4561 acggacgcga tcagtccgcc atccccttcg accgaggggg caggagggaa tcatcgcccg
     4621 cgccgcccgg attcccgacg atcggaaggc cccacgcagg acgacggagg ttctcgagaa
     4681 cctcttcgaa ggcaaccgat cccgccgggg aagttcaaca agaggaagct gaacaaggcc
     4741 ctcctggccg gcatgggcaa gaaggtgagg ggcaggatcc tcaactggaa gctgacataa
     4801 agggagggga gggggcccgg ctggagcctg gagcggcccc gacccgatcg gcgcgcgtct
     4861 atcctctcgg ggtgatcatc gacgagccgg tgcgaggacg ctacgcgtac ctcgagcacc
     4921 cgtggctctt cgccctcccc ccgcacgagc agatgcggcc gttcatcgaa ggcgacgtcc
     4981 ctcgtccgcc gatccaccac ctcacgggtc tgataccggt cgaggcgggt ccgacgtcgt
     5041 cggtctggtc catgccggcg agcccgtggt ggaggtcagc ggccggcatc ttccccggcg
     5101 gcgcgctcgc gatcgtcgcc gacgccgcgc tcagcggagc gatctacggc ggcctcccga
     5161 agggcaccgc cctcgcgacc tccgagctga cgatgaactt cgtccgtccg gccacctccg
     5221 agagcggtga gctgatcgcc accggcagct tgatccactc gggctcgcgg cagggcctct
     5281 ccgacggacg gatccgggac gccagcggcc ggctcctggc ccacgccacc tcccgatgcg
     5341 tgctgcgcag gctcccgttc gatccgcccg atcccccggc cacgctgccg ccgtacaagc
     5401 agtccgacca cgacacgccc gacccttacc ttcgcccgcc cgagggcgtg gtgatccccc
     5461 aggaggagtg ggacgcgtcc cccggcctcg acctggtccg ttcgtggagc ggcggcgctc
     5521 gagggacccc acccgtctgc cggctctcgg gctggcggct ccgggaggcc gaggagggcg
     5581 ccacgacgtg ggcgatgccc gctagcgaat ggttctgcac cggcttcggg accttctacg
     5641 gggggatcat caccctgttc ctcgacggcg cgatcaacac cgccgtgacg accacgcttc
     5701 ctccgcgggc gtccttcggc acgctggatc tcaaggtgaa cttcctgcgc ccggtcagcc
     5761 ccgacggaag ggacctcgtc gtccgggcca aggtcgcgca tcggggccgg accgtggccg
     5821 tgaccacggc ggagatgcac gacgccgacg ggaagcgggt tgcgatggcc accagcagcg
     5881 ccatgatcct cccgggcgtc ccctggaacg ccgcgaagcc cccggagacc ctcgacgagg
     5941 caccggacga gcagggctga gccgcgccca cgcttgacag gcaagtccct acttgcctgt
     6001 cgtggcggca cgagtgtcgg acgtgtaccg ggcgatcgcc gacccgtcga ggagagccat
     6061 cctcgacgag ctggtcgaca gggacgggca gacgctgttc gaggtctgcg cccggctggc
     6121 catgaggcac gggctcacgt cgtctcgcca ggccgtctcc cagcacctcg cggtgctgga
     6181 ggaggcgggg ctcgtgagga ccaagcgaga gggccggtac acgttccact tcctcgacac
     6241 acgcccgctc ggcgaggtga tcgagcggtg gccgatcgag aggaggacgc gatgaggatc
     6301 aacctgacgt ccgtctacgt ggacgaccag gagaaggccc tgcgcttcta cacggaggtg
     6361 ctggggttcc ggaagaagag cgacatgccg ctcggagagg gcgcccggtg gctgaccgtg
     6421 gtctcgccgg aggatcccga cggccccgag ctactgctcg agccgagcga acatcctgcg
     6481 gcgacgccgt tcaaggaggc gctcgtctcg gacggcatcc cgttcacgtc cttcgccgtg
     6541 aaggacgttc gggccgaggc ggagcggctg aagggcctcg gggtgcggtt cacgcaggag
     6601 cccgtcgaga tggggccggt cacgatggcg gtcttcgacg acacctgcgg gaacctgatc
     6661 cagatcgcgg ccacctagga cttcgcctcg tcctccagcg gctccacgtc gaaggggccg
     6721 tggaagatcg tgacgaacaa gcatcccgca tcgtcggcgg gggcatggcg catcgcctcc
     6781 cccgccggga agtggcagta agcgcccggc ccgactacga cttcgttcac tcgcagccgc
     6841 ccctcgagca cgacgaccgt gtgcgaggcg gtgtgtcggt ggaggcgggt cctgaggccg
     6901 gccgggtacc ggacgaggta gtgctcggcg cccgaggctg ggtcctgata caggagccgg
     6961 agaccgatct cccgatcgta gatgacgggg tgggacggcc tcagctccag cgaggcgagc
     7021 gcgtccgcga tgatcgctcg gcccggctcc atgcgtcatc ctggcacagc ccccccggac
     7081 caccgcggcg aaagcggtgg gtcgctttgc cgaaatcgcc ttgcttcgat agggtcgctt
     7141 cttccacccg aaaggaggcc tcatgaggag gctgatcgtg tccgtggcga ccctgggcgc
     7201 catcgccctg gcgtcgcccg cgagcgccgt accgccccag cacttctccg tcgatccacc
     7261 gcccaacatc gtcgaggaca acggcgaagc ctgcgggttc ccggtccgct ggacgatcga
     7321 cctcgaggtt caaggcacga acttcttcga ccaggacggg gtcatcgtgc gccagcaggc
     7381 ccacgtccag gaggacaaca cgatcgagaa cctggacacg gggctgacgc tgcgggaggg
     7441 tccggactcg ttcatgcaga cgatccactt cgagaacggc gtgccgaccc acttcgtcgc
     7501 gacggggctc ggtctcaacg tcttcggcga gcagctgaag gacgtgggac gcgtcgcgtg
     7561 gctcccgggg acgaacgaga tcatcttctc ggccggcccc cacgaagctc gagagctgct
     7621 ccagttcggg accttcgagg acgtcctggc gttcttctgc gacgtgctcg cctgatccct
     7681 gctcgatcgg ggcgcggcgc ccggcgggat ccatcccgtc gggcgccgcg ctacgcttcc
     7741 ccccgcatgc gcatcctcgt ctggcacggc tggatcctcg agggatcggg ctccaacgtc
     7801 tacacggcga ggatcgtgga agcgtggcgg gcgcgggggc acgacgtcct cctcgtggcc
     7861 caggagcggc acccggagcg cttcgccttc ctcgacgcgt gggccgacgg gaacctcacc
     7921 gagcctcacc ccacgggcgt cgagccctcc ctcggccgcg ccaccctcct ccgccccgac
     7981 atcggcgatc tcctcccggt gttcgtctgg gacgcgtacg aggggttccg cgtgaagacc
     8041 ttcgtggatc tgagcgacga ggagctcgaa ggctacctcg accggaacgt ggcggccctg
     8101 cgcgaggcgg cggaggctca cggcacggac gtcgcgatcg ccgggcacgc gatcccgggc
     8161 gccaacgtgg cccggcgcgc cctcggctcg atccccttcg ccgccaagat ccacgggagc
     8221 gacgtcgagt acgcgatgcg cggacagcgg cggtacgtgg agctcgcgcg cgagggcctg
     8281 gagggcgcgg tggtgatcgc cggcgcctcg cgcgacgtgc tcgaccgtct catcgagctc
     8341 gtgccctcgg tggccggccg gaccaaggtc gtccccccgg gcgtggaggt cgagcgcttc
     8401 cacccgatgc cccgcgccga agcgctgcgc ggagcggcga gcctgctgga gcgggacccg
     8461 gccctggcac ggggccggcc ggagacggcc gcgagcgacg ttgccgaggc gctccgacga
     8521 cgagacggag atgcactgga caggctggcg aacacctacg agcagacctc ccccgatcgg
     8581 gacgcacccc gacggctccg ggcgctcgcc ggctacgacg gtccgctcgt gggctacctg
     8641 gggaagctga tcccccagaa gggcgtgcat gacctgctcg ccgctctggc gctcctgccc
     8701 tcgaggccca gggcgctcgt cgtcgggttc gggaccttcc gggagtggct cgaagccctg
     8761 gtcgccgcgc tcgacgcggg cgacccggtg gcggcggcgt tcctggagga gcggatgccc
     8821 ctcggcctct cccccgggga ggtggccgcg gcggccggcc tggccgagcg ggtcgtgttc
     8881 acgggacgcc tcgaccaccg ctacgcgggg ccggccgccg cggccctcga cgtcctcgtc
     8941 gtcccgtcga tcctggagga ggcgttcggg atggtggctt tggagggggc ggcggcgggc
     9001 gcgctgccgc tggtggcccg gcacagcggg ctggccgagg tggccgatgc cctcgagacc
     9061 gagatcggcc ggccgggcct cctctcgtac gagcctgggc tttcggcccc ccgagccctg
     9121 ggccgcgccc tggaggggct tctggccctc ccggaccgcc gtgagctggc cgctcacctc
     9181 tcgtcccacg tccgggagcg gtggagctgg gggcgcaccg ccgacctcct cctggagccc
     9241 ctccgctagg tggcgaggag ggccgcgatc gcgtcgacct ccccctcgat cctcccccgg
     9301 gtccgggggc ccagcgtgtc gaacggctgg acctcggccc cgtccttcag cgagtacgtc
     9361 caggtggcca cgacctcgcc gtccacgagc acgaccgggt tgccgtcgcc gggcaggccc
     9421 accccgatcg gctcgtacac ccgctgctgg acgtcagggt gcacgaaccg ggcccggccg
     9481 tcgggcgcgt gccccatcgt gtacgcgtcc cacctcggga gcagggcgag ggtgcctttc
     9541 agtcggcggg tgcgctcgaa ggccgcctcg tggcgggccg gcagcagcag accttcgccg
     9601 gcgtcgatcg tgtcgtgctc tgcgaccgcg gcggccgagg cgcgcttgcc cacgcccgcc
     9661 caccaggcga agtcctcgac ccgggccgga ccgtaggcct cgaggtaggc gccggccagc
     9721 caggccaggg cctcctcccg gtctcccgcg tcaagtccgt cctccgccca ggcctcggtc
     9781 gcgacgtacc ggtgggggct cgaggtcagg ggctcgcccg ccagggcgag cagccgcccc
     9841 tcgaggcgca ggccgcggag gatcgtcgcc aggaccgggc cctccagtcc cgtcgcctca
     9901 cggagatcct ccgccctggc cggctccccg gcgacggcca ccacccgtcg cgcgatcgcc
     9961 tcgtactcct cctccgacat gtcgtgccgc ttgagcctgg ccacgacctt cgaccgggga
    10021 ggccggacgg cggtgaagat tcgagcggcc gcctcgcgcg ggacgaggaa gaccgtgccc
    10081 cgcatgccgg ggatccgcag gcctcgccgc tcgcggtcca gccgccggta cgcggtgccc
    10141 gtgagggacc gcgtccgggc ccggagggcc aggggggcgg tggggtgcgt ggcgtagacg
    10201 gcgaccacgg ccctgagggc ctcgagggct cctcgggcgg gccggcccag gcgctggcgc
    10261 gcgtaggtcc aggcccgaac gcgctccagc actcagtcct ccggagagcg gaaccgcagg
    10321 gccaccccga tcacgatcac ccccacgatg aacgcgagcg tgttgggcac gatcagggcc
    10381 acgttctcga tggcgagccc gtagacgatc cagagggcga agccgatcgc gatgacggtc
    10441 aggtacccga tggagacgtc cctcgacgag cggtaagtca ggatccggcg gatctgaagg
    10501 acgggcgaga gcgccatcac gaccccccac gaagcggcga cgaccccgag cgcggtctcg
    10561 ttcacgatga acctcctgtg caaacgacgg tgcgctcggt acgagggctg cggtcgctgc
    10621 cgtgggcagg aggagcgcac ccgcagccgt ttcgagtcta ccaacgtgag cgcgctcgag
    10681 aaagagtgac aagacgagcg tcccgggttg cgcccgcacg tccgggcggt gtacaaacgc
    10741 ttcggaaccg ctggggtctt cgggccccgg cggttccgtc atctccgccc gactttcctc
    10801 caccggtcct gctg
//
LOCUS       DASUTZ010000067         7448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1445035, whole genome shotgun sequence.
ACCESSION   DASUTZ010000067 DASUTZ010000000
VERSION     DASUTZ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7448
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445035"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VF097_03090"
     CDS             complement(<1..492)
                     /locus_tag="VF097_03090"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX6261815.1"
                     /translation="MLRAVDVGNTETVLGVFSDRDLTRQWRISTVADRTADELALMFG
                     GLLEHQDLSFDRNVTAVVIASVVPTATQALREMVRRYFGFRPLVVEPGVKTGIPVLTD
                     NPKEVGADRVLNAVAAIDLHGSPAVVVDFGTATTFDAVSEKGEYVGGVIAPGVQISAA
                     ALYE"
     gene            complement(494..1357)
                     /gene="nadC"
                     /locus_tag="VF097_03095"
     CDS             complement(494..1357)
                     /gene="nadC"
                     /locus_tag="VF097_03095"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014515620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX6261816.1"
                     /translation="MIELSREVVANAVLAALREDLGAEGDLTSAAVIPEVQTARARIV
                     SRSRGVLAGIPVAKECFSRVNARLRPLKEDGDRIVPGDEVAVVGGALRAILAAERTAL
                     NFLGRLSGVATQASRFVEAVRGTGVVVRDTRKTTPGLRLLEKYAAELGGATPHRAGLF
                     DGVFIKDNHVAAAGGVAEAVRRAKVARPDLPVVCEVETSDQAEEAASAGAEDVLLDNM
                     SLEAMRAAVERVGGRARVEASGGVTLDTARAVAETGVDALSSGAITHAAPWIDLSLEV
                     LEDVDRPAEEG"
     gene            complement(1354..2196)
                     /gene="panC"
                     /locus_tag="VF097_03100"
     CDS             complement(1354..2196)
                     /gene="panC"
                     /locus_tag="VF097_03100"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX6261817.1"
                     /translation="MIRRREPLRDAADDGRLEGRTVGFVPTMGFLHEGHLSLVRKARR
                     EADFVVASIFVNPLQFGPGEDLDAYPRDEARDLELLEEAKTDLAFVPAVEEMYPDGSP
                     AVTIDPGGLGDVLEGASRPGHFRGVCTVVAKLFGIVGPCRAYFGEKDAQQLAVVRRMV
                     GDLDLPVSVLSGETVREPDGLAMSSRNVYLSPEEREAARAIPRALAAAEALVDGGERD
                     AHVVRAEMAKHVGVEPTAELDYVAVVDEATFEGVDRLERPARALVAARFGRARLLDNV
                     RLAP"
     gene            complement(2193..3059)
                     /locus_tag="VF097_03105"
     CDS             complement(2193..3059)
                     /locus_tag="VF097_03105"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEX6261818.1"
                     /translation="MKVAVVGAGRVGTAMGVLLSRAGHTVVAVSGRGVGSRERAERHL
                     PGTAFVGSVEAARRGEVVLLTVPDDRILDVCGTLVKRGALESKPSLVHCSGANPLQDL
                     APAAEIGASILSVHPLQTAPSVEAAIERFPGSGFAVTARDEDSFLLGERLAADAGGRP
                     FRLLDEHKGLYHAAAVFASNYLVVVEGLADDLFELAGIPDPRPLFGPLAEGTLANVME
                     QGAARALTGPVARGDAGTVRRNLEALRVRAPSCVAPYAELARAAVRIASEAGRLNDRD
                     RAEIEEVLREWT"
     gene            complement(3123..3701)
                     /locus_tag="VF097_03110"
     CDS             complement(3123..3701)
                     /locus_tag="VF097_03110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6261819.1"
                     /translation="MTLQVRPVTPDRWDDLVALFGLDRGAYSGCWCMWWRQTAKEYDR
                     DHGAPNREAMRAIVREGREPGLLAYRRGEPVGWVSLGPRGDFGRLDRSRVLGRVDDEE
                     GVWSIVCFYIHRRHRRDGVAKALLAAAVARAAERGARCVEAYPVDLGAAAAKKASAEL
                     FTGTLAMFEDAGFREVARRGTARPIVRAPALR"
     gene            complement(3698..4243)
                     /locus_tag="VF097_03115"
     CDS             complement(3698..4243)
                     /locus_tag="VF097_03115"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEX6261820.1"
                     /translation="MSTDLDLVAAFVNTLDVESGHDALESAEALRGWLVQRGLLGGDH
                     PVTDADLGRALAVREAIRGLAERNNPGAGTPPDAEPVETLNRLADGSRLILSFGPEGD
                     ARLESVDEGVPGALGRILAAVFVAMTDGRWDRFKACARDSCRWVYVDRSRNRSRTWCS
                     MDVCGNREKARAFRERRKAKR"
     gene            4379..4543
                     /locus_tag="VF097_03120"
     CDS             4379..4543
                     /locus_tag="VF097_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261821.1"
                     /translation="MQGHTIWELSRMHAADLLLDAGPVRRRPRRRMVRRAVGTGMVRA
                     GYRLIGARAS"
     gene            4540..4857
                     /locus_tag="VF097_03125"
     CDS             4540..4857
                     /locus_tag="VF097_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX6261822.1"
                     /translation="MIARIWTGQTRAEDAEAYAGYLARTGFPDYRATPGNRGVLGLRR
                     IEGDRATFTLITFWDSIDAIRAFAGEDPERARYYDEDDRFLLSFPERASHYEVIEGRQ
                     TAR"
     gene            4854..5510
                     /locus_tag="VF097_03130"
     CDS             4854..5510
                     /locus_tag="VF097_03130"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEX6261823.1"
                     /translation="MKSVEQGYRQGARAILPLVVPVVGFGVAFGVLAREAGMGILAPV
                     VMSATTFAGSAQFAASSILGVGGGAVAAIVAALLLNARYTPIGLTVAGAIEGPSWLRF
                     LRSQLIVDESWAVATTPEGVDGGRLIGAGLVLYATWVTSTLVGALGARILGDPATLGL
                     DAAFPAVFVALLVPQLTGHRPRLAAGLGALIALALLPWAPAGVPVIAATAAILVGWSR
                     "
     gene            5507..5809
                     /locus_tag="VF097_03135"
     CDS             5507..5809
                     /locus_tag="VF097_03135"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEX6261824.1"
                     /translation="MKIWVVVAAAGAGTAVLKAAGPLLLHGRPMSPRAQRMLSLLAPA
                     LLSALILVQVLGVEDGIRFDERLLGVGAAIVAAALRAPIWVVVGVAALVTALARLP"
     gene            5815..6183
                     /locus_tag="VF097_03140"
     CDS             5815..6183
                     /locus_tag="VF097_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018779814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX6261825.1"
                     /translation="MVERDDVTRWIEGYERAWTSNERGDIEALFTEDARYFTAPYRKP
                     WEGREAIVEGWLHDRDQPGQWRFASEVLAIAGELAFVQGRTEYLDEPRRAYSNLFVIR
                     FSGDGRASVFTEWFMPIKDT"
     gene            complement(6180..7223)
                     /locus_tag="VF097_03145"
     CDS             complement(6180..7223)
                     /locus_tag="VF097_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6261826.1"
                     /translation="MGYARIVVGTDGSETAGRAVDRAARLSKDLGSTLILVTASAPIG
                     MIDTVAEEIAVAARDRVRAKGIEAEAVVQEGDPAEVLTAVAAAQDAGLVVVGDQGLGR
                     ARRFRLGGVAERVAVMAPCDVLIVETQNPREEPPEGPLYRRLLVGTDGSPTATAAVQR
                     AYDFGMMIGVGVLVATVTDDDVLAAIHLEDARKIKPRALGVEAKVLRGEPAKALVKEA
                     DREGCELIVVGNRGLTGARRVLLGSIPSRVAHRADRDVLIARTVGRTIKDLRPGQGAV
                     VDAGSGKMVAAFLDEDGGVLALDPRCTHMGCTVRFNQSERTWDCPCHGSRYDLEGQVL
                     QGPAKKPLSKTTL"
     gene            complement(7223..>7448)
                     /locus_tag="VF097_03150"
     CDS             complement(7223..>7448)
                     /locus_tag="VF097_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261827.1"
                     /translation="SIGILVAIIVTMYAIAGPYYRRVRTIVQAMARGSEAVTPQQLDE
                     ILRAPSALWNAIFGFAALLAIPYLMVFKPF"
BASE COUNT         1015 a         2849 c         2561 g         1023 t
ORIGIN      
        1 ctcgtagagg gcggcggccg agatctgcac gccgggcgcg atcacgccgc cgacgtactc
       61 gcccttctcg gagacggcgt cgaaggtcgt ggcggtcccg aagtcgacga ccacggccgg
      121 ggagccgtgg aggtcgatcg cggcgacggc gttgagcacc cgatccgcgc cgacctcctt
      181 cgggttgtcg gtcagcacgg ggatcccggt cttcaccccg ggctccacga cgagcgggcg
      241 gaagccgaag tagcggcgga ccatctcgcg aagggcctgg gtcgcggtcg gcacgacgct
      301 ggcgatcacc acggcggtga cattgcgatc gaaggagagg tcctggtgct cgaggaggcc
      361 cccgaacatc agggcgagct cgtcggcggt gcggtcggcc acggtggaga tgcgccactg
      421 gcgcgtgagg tcacgatcgc tgaagacccc cagcaccgtc tcggtgttgc cgacgtccac
      481 ggcccgcagc atctcagccc tcctccgcgg gccggtccac gtcctcgagc acctcgagcg
      541 acaggtcgat ccagggcgcg gcgtgcgtga tcgcgccaga cgacagcgcg tccacgcccg
      601 tctccgcgac cgccctcgcg gtgtcgaggg tcacaccgcc cgaggcctcg acccgggccc
      661 gcccgcccac ccgctcgacg gctgcccgca tcgcctcgag tgacatgttg tccaggagca
      721 cgtcctcggc gcccgccgac gccgcctcct cggcctggtc cgacgtctcg acctcgcaca
      781 cgaccggcag atcggggcgc gcgaccttcg cccggcgaac cgcctcggcc acgccccccg
      841 ccgcggccac gtgattgtcc ttgatgaaca ctccgtcgaa cagcccggcg cgatgcggcg
      901 ttgccccgcc cagctccgcc gcgtacttct ccaggaggcg gaggccgggc gtggtcttcc
      961 gcgtgtcgcg gacgacgacg ccggtgccgc ggaccgcctc cacgaaccgc gaggcctggg
     1021 tggcgacgcc cgagagcctc ccgaggaagt tgagggcggt gcgctccgcg gcgaggatcg
     1081 cgcggagcgc accgcccaca accgccacct cgtcacccgg gacgatccga tcgccgtcct
     1141 ccttcagagg gcggagccgc gcgttcaccc gcgagaagca ctccttcgcc acggggatcc
     1201 cggccagcac ccctcgggac ctcgagacga tccgggcccg agccgtctgg acctccggga
     1261 tcaccgcagc cgacgtcagg tcaccctcgg cgcccaggtc ctcccggagc gccgccagga
     1321 cggcgttcgc caccacctcc cgcgacagct cgatcacggc gccagcctca cgttgtcgag
     1381 gaggcgagcc cggccgaacc tcgccgccac gagcgcccga gcggggcgct ccaggcgatc
     1441 gaccccctcg aacgtcgcct cgtccaccac cgccacgtag tccagctccg ccgtgggctc
     1501 gacccccacg tgcttcgcca tctcggcccg cacgacgtgc gcgtcccgct cccctccgtc
     1561 gaccagggcc tccgcggccg ccagggcccg cgggatcgcc ctcgccgcct cccgctcctc
     1621 cggagagagg tagacgttcc gcgaggacat cgccaggccg tcgggctccc gcacagtctc
     1681 ccccgacagc acggagaccg gcagatcgag gtccccgacc atccgccgca cgaccgcgag
     1741 ctgctgggcg tccttctcgc cgaagtaggc tcggcacggc cccacgatcc cgaagagctt
     1801 cgccacgacc gtgcagaccc cccggaagtg gccgggccgt gaggctccct ccaggacgtc
     1861 gcccaggccc ccggggtcga tcgtgacggc cggcgatccg tccgggtaca tctcctcgac
     1921 cgccgggacg aaggcgaggt cggtcttggc ctcctcgagg agctcgaggt ccctggcctc
     1981 gtcccggggg taggcgtcca ggtcctcgcc cggaccgaac tggagcggat tcacgaagat
     2041 cgaggccacc acgaaatcgg cctcgcggcg ggccttccga acgagcgaca ggtgcccctc
     2101 gtggaggaac cccatcgtgg gcacgaagcc cacggtgcgg ccctcgagcc ggccgtcgtc
     2161 cgccgcgtcg cggaggggct cgcggcgccg gatcacgtcc actcccggag cacctcctcg
     2221 atctcggccc ggtcccggtc gttcaaccgg ccggcctcac tcgcgatgcg caccgcggcc
     2281 cgggccagct cggcgtaggg agccacgcac gacggcgccc ggacgcgaag ggcctcgagg
     2341 ttcctccgga cggtccccgc gtcgcctcgg gccaccgggc ccgtgagcgc gcgggcggcg
     2401 ccctgctcca tgacgttcgc cagcgtcccc tcggccaggg gcccgaacag cggcctggga
     2461 tcggggatcc ccgccagctc gaacaggtcg tcggccaacc cctccaccac caccaggtag
     2521 ttcgaggcga acaccgccgc cgcgtgatag agccccttgt gctcgtccag gagccggaag
     2581 ggcctcccgc cggcgtcggc ggccagccgc tctcccagca ggaacgagtc ctcgtcgcgc
     2641 gccgtcacgg cgaagccgct cccggggaag cgctcgatcg cggcctccac gctgggagcc
     2701 gtctggagcg gatggacgga gaggatggag gccccgatct ccgccgccgg ggcgaggtcc
     2761 tggagcgggt tcgctccgga gcagtggacg agcgacggct tcgactccaa ggctccgcgc
     2821 ttgaccaggg tgccgcacac gtcgaggatc cggtcgtcgg ggacggtgag gagcaccacc
     2881 tcgcccctcc gggcggcctc cacggagcca acgaaggccg tcccgggcag gtgcctctcc
     2941 gcgcgctcgc gagagccgac cccccggccg gagacggcca cgaccgtatg ccccgcgcgg
     3001 gagaggagca cccccatcgc ggtcccgacc ctgcccgccc cgacgacggc caccttcacg
     3061 gggtcgcgtg ggtctcgagt ggacgcgcgc ggacgatcgt gcttcgacgc gaagtataga
     3121 ggtcagcgga gggctggggc ccggacgatg ggccgtgccg tcccgcgccg ggccacctcg
     3181 cggaacccgg cgtcctcgaa catcgccagg gtgccggtga agagctccgc cgaggccttc
     3241 ttcgcggcgg cggcccccag gtccaccggg taggcctcca cacatcgggc cccccgctcc
     3301 gcggcgcggg ccacggcggc cgccaggagg gccttggcca ccccatcccg ccggtgccgg
     3361 cggtggatgt agaagcagac gatcgaccag accccttcct cgtcgtccac gcgccccagg
     3421 acgcgcgagc gatccagccg cccgaagtcg ccccgcggcc cgagcgacac ccatcccacg
     3481 ggctcgcccc ggcgatacgc gaggagcccg ggctccctcc cctcccggac gatggcgcgc
     3541 atcgcctcgc ggttcggcgc gccgtggtcg cggtcgtact ccttcgcggt ctgccgccac
     3601 cacatgcacc agcagccgga gtacgcgccg cggtcgaggc cgaacagcgc caccaggtcg
     3661 tcccagcggt cgggggtcac cggccggacc tggagggtca ccgcttcgcc ttcctccgct
     3721 cccggaatgc ccgggccttc tcgcggttgc cgcagacgtc catcgagcac caggtccgtg
     3781 agcggttcct cgagcggtcg acgtagaccc agcgacacga gtcgcgggcg caggccttga
     3841 agcggtccca tcggccgtcg gtcatggcga cgaagacggc ggccaggatc cggcccaggg
     3901 ccccgggcac cccctcgtcc accgactcga ggcgcgcgtc cccctccggg ccgaacgaga
     3961 ggatcagcct cgacccgtcc gcgagccggt tcagggtctc caccggctcc gcgtccgggg
     4021 gcgtccccgc gcccgggttg ttccgctcgg cgaggccgcg gatcgcttcc cggacggcca
     4081 gagctctccc caggtcggcg tcggtgacag gatggtcgcc ccccagcagg ccgcgctgga
     4141 ccagccagcc cctcagggcc tccgcgctct ccagcgcgtc gtggccggac tccacgtcca
     4201 gcgtgttcac gaacgccgcc acgagatcga ggtccgtcga catcccaacc agtgtatccc
     4261 tctcgttggt tatggagcaa gcgttccgcg ctcgctctcc taaccttcgt cggtacttga
     4321 caggtgatgc gccaccagtc taccatgatg accggttacg acaggcagag gagggaagat
     4381 gcagggtcac acgatctggg agctctcccg gatgcacgcc gccgacctgc tcctcgacgc
     4441 cggacccgtg cggcgccggc cgaggcggcg gatggtcagg cgggccgtgg ggaccgggat
     4501 ggtgcgggcc gggtaccggc tgatcggagc gcgggcctcg tgatcgctcg catctggacg
     4561 gggcagacgc gggccgagga cgccgaggcc tacgcgggct atctggcccg gaccgggttc
     4621 cccgactacc gcgccacgcc ggggaaccgc ggcgtcctcg gcctgcgccg gatcgagggt
     4681 gaccgggcga ccttcacgct gatcaccttc tgggactcga tcgacgcgat ccgcgccttc
     4741 gccggagagg accccgagcg cgcgcgctac tacgacgagg acgaccggtt cctgctctcc
     4801 ttccccgagc gggcctccca ctacgaagtc atcgagggtc gccagacggc aagatgaagt
     4861 ccgtggagca ggggtaccgg cagggcgctc gcgccatcct ccctctcgtc gttcccgtcg
     4921 tggggttcgg cgtggccttc ggcgtgctcg cgcgggaggc gggcatgggg atcctggccc
     4981 cggtcgtgat gtcggccacg acgttcgcgg gatcggcgca gttcgcggcg tcctcgatcc
     5041 tcggcgtcgg cggcggcgcc gtggcggcca tcgtcgccgc gctcctcctc aacgcgcgct
     5101 acacgccgat cgggctcacc gtggcggggg cgatcgaggg accgagctgg ctccgcttcc
     5161 tccgatccca gctgatcgtg gacgagtcct gggccgtggc caccaccccg gagggtgtgg
     5221 acggcgggcg gctgatcggc gccgggctcg tcctctacgc gacctgggtg acctcgaccc
     5281 tcgtgggcgc tctcggcgcg aggatcctgg gggaccccgc gacactcggc ctcgatgccg
     5341 ccttccccgc cgtgttcgtg gccctgctgg tcccccagct cacgggacat cgtccccggc
     5401 tcgccgccgg cctgggcgcg ctgatcgccc tggccctgct cccgtgggcg ccggcgggcg
     5461 tcccggtgat cgcagccacc gccgcgatcc tcgtggggtg gtcccgatga agatctgggt
     5521 cgtggtggcc gcggcggggg cggggaccgc ggtcctcaag gcggcggggc ccctgctcct
     5581 ccacggtcgc ccgatgtctc ccagagccca gcggatgctc tccctgctcg cccccgccct
     5641 cctctccgcc ctgatcctgg tccaagtgct cggggtcgag gacgggatcc gcttcgacga
     5701 gcgtcttctc ggggtgggcg ccgcgatcgt ggctgctgcg ctccgggcgc cgatctgggt
     5761 cgtggtcggg gtggcggccc tcgtgaccgc gctggctcga ctaccctgag ggggatggtc
     5821 gagcgagacg acgtgacccg ttggatcgag ggctacgagc gggcttggac ctccaacgag
     5881 cgcggggaca tcgaggcgct cttcaccgag gatgcgaggt acttcaccgc gccctaccgc
     5941 aagccgtggg aggggcgcga ggcgatcgtc gaggggtggc tccacgaccg cgaccagccc
     6001 ggacagtggc gcttcgcgtc ggaggtgctg gcgatcgccg gcgagctggc cttcgtccag
     6061 ggacggaccg agtacctcga cgagccccgc cgcgcgtact cgaacctgtt cgtcatccgc
     6121 ttctcggggg acgggcgcgc ctccgtgttc acggagtggt tcatgccgat caaggacacc
     6181 taaagggtcg tcttcgagag cggcttcttg gcgggcccct gcagaacctg cccttccagg
     6241 tcgtagcgcg agccgtggca cggacagtcc cacgtccgct ccgactggtt gaagcggacc
     6301 gtgcacccca tgtgggtgca gcggggatcc agggccagga cccccccgtc ctcatccagg
     6361 aaggccgcga ccatcttccc gctcccggcg tccacgaccg cgccctgccc cggcctgagg
     6421 tccttgatgg tccgccccac ggtccgggcg atcagcacgt cacggtcggc gcgatgcgcg
     6481 acgcggctgg ggatcgatcc gagcaggacc ctgcgggccc cagtcagccc ccgattcccg
     6541 accacgatga gctcgcaccc ctcccggtcg gcctccttca ccagagcctt ggccggctcg
     6601 ccgcgcagca ccttcgcctc cacccccaga gcccggggct tgatcttccg cgcgtcctcg
     6661 aggtggatcg cggccaggac atcgtcgtcg gtcaccgtgg ccacgagcac cccgaccccg
     6721 atcatcatcc cgaagtcgta cgcgcgctga accgcggccg tggccgtggg cgagccgtcg
     6781 gtccccacga ggagacggcg gtagaggggt ccttcggggg gctcctcccg cgggttctgc
     6841 gtctccacga tgaggacgtc gcagggcgcc atcaccgcca cccgctcggc cacgcccccg
     6901 agcctgaacc ggcgggccct tcccaggccc tggtccccca ccaccaccag cccggcgtcc
     6961 tgcgccgcgg ccaccgcggt gaggacctcg gccggatccc cttcctgcac gacggcctcg
     7021 gcctcgatcc ccttggcccg cacccggtcg cgagccgcga cggcgatctc ctcggccacc
     7081 gtgtcgatca tcccgatcgg ggcggaggcc gtgaccagga tcaaggtgct tcccaggtcc
     7141 ttggacagcc gggccgcccg gtccacggca cgtccggcgg tctcggagcc gtcggtgccg
     7201 acgacgatcc tggcgtagcc catcagaacg gcttgaagac catcaggtag gggatggcga
     7261 gcaacgccgc gaacccgaag atcgcgttcc agagcgcgga cggggcacgg aggatctcgt
     7321 cgagctgctg aggcgtcacg gcctcggagc cgcgggccat ggcctggacg atcgtcctca
     7381 cgcgccggta gtaggggccg gcgatcgcgt acatcgtcac gatgatagcc acgaggatcc
     7441 cgatcgag
//
LOCUS       DASUTZ010000068         2739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1315212, whole genome shotgun sequence.
ACCESSION   DASUTZ010000068 DASUTZ010000000
VERSION     DASUTZ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315212"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2186
                     /locus_tag="VF097_03155"
     CDS             <1..2186
                     /locus_tag="VF097_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX6261828.1"
                     /translation="RFSADEASAIAARLFGVEGPATPLASERDQGFLLGGGVLKISNA
                     GERPEVLDMETAAALHVRRVDPDLPLAMPIPLVGTDPPAYRAEVDGHLIRMFERLPGR
                     ASVPGAELGLDAVRAFGEMLARLGRALRGFFHPAAGRVILWDVQHALSLRPLLREIED
                     EGARALADRALRRFGERALPAWPHLRAQVIHGDLSLDNALLDEEGRVTGIVDLGDMSH
                     TALLCDLSSALASVLGGRDDVFRAARVLVDGYERITPLEPEEIELLPILLGARLAATV
                     VISAWRVRRYPENAEYIGGWDEETWPLLRLLDEVGAEEAAARLGAPRVAPTEDLLERR
                     RRLLGPALSPLTYRRPVHAARAEGVWIVEADGRRLLDAYNNVPTVGHSHPRVAEAVAR
                     QSRRLATNARYLYEPLIELAERLAGTMPAGLDTVTLVNSGTEANDLAWRMAVAWTGAS
                     GGIVTAHAYHGVSAVTADLSPETWGEPPDHVETFGPPGEGAGPAIERLGARGLEPAAL
                     FLDGGFTSDGILVPGSPYVRDLAREIRSAGGLFVADEVQVGHGRTGEHLWSFAADGVT
                     PDLVTMGKPMGNGFPVAAVVTRREVAARLGESTEVFSTFGGNPVAAAAALAVLDVIED
                     EGLIERAAEVGGLLLEELRALARRHDEVRDVRGRGLLIGVELASPAAPVADAMREAGV
                     LVGVTGPRGDVLKVRPPLGFGPDHATRLVETLDRILALRDATPGE"
     gene            2233..2673
                     /locus_tag="VF097_03160"
     CDS             2233..2673
                     /locus_tag="VF097_03160"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX6261829.1"
                     /translation="MDALKLLKEDHEKVKKILDELESTTERGVKTRQDLFERLRRELD
                     VHEAIEEEILYPALKEHPKTKELALEAYEEHHVVDMVMEELEDVPFDDESWGAKLMVA
                     KENLEHHIEEEEGEMFQQARQVFDDEELGELGDRMEARKSELLG"
BASE COUNT          364 a          959 c         1050 g          366 t
ORIGIN      
        1 cgaggttctc ggctgacgag gcctccgcga tcgcggctcg gctcttcggc gtggaggggc
       61 cggcgacgcc gctggccagc gagagggacc agggcttcct gctgggcgga ggcgtcctca
      121 agatctcgaa cgccggcgag cggcccgagg tcctcgacat ggagacggcc gcggcgctgc
      181 acgtccggcg ggtggacccc gacctgcccc tggcgatgcc gatcccgctg gtggggaccg
      241 acccgcctgc gtaccgtgcc gaggtcgacg ggcacctcat ccggatgttc gagcggctgc
      301 cggggcgcgc ctcggtgccg ggggcggagc tcggcctcga cgccgtgcgg gccttcggcg
      361 agatgctggc ccggctgggg cgcgccctgc gcggcttctt ccacccggcg gcggggcggg
      421 tcatcctgtg ggacgtccag cacgcgctct ccctccggcc gctcctgcgc gagatcgagg
      481 acgagggcgc tcgggcgctg gccgaccggg cgctgcgtcg gttcggggag cgagccctcc
      541 cggcgtggcc tcaccttcgg gcccaggtga tccacggcga tctctcgctc gacaacgcgc
      601 tcctcgacga ggagggccgc gtgaccggga tcgtggacct cggggacatg agccataccg
      661 cgctcctgtg cgacctgtcg tcggcgctgg cgtccgtgct ggggggtcgc gacgacgtgt
      721 tccgggcggc tcgggtgctt gtggacggct acgaacggat cacgccgctg gagcccgagg
      781 agatcgagct gctgcccatc ctgctcgggg cgaggctggc ggccaccgtg gtgatcagcg
      841 cctggcgcgt tcgccggtac ccggagaacg ccgagtacat cgggggctgg gacgaggaga
      901 cctggcccct gctgcggctg ctggacgagg tgggggccga ggaggcggcg gcgcggctgg
      961 gagcgccgcg ggtcgcgccg accgaggacc tgctcgaacg gcgccgccgc ctcctcggcc
     1021 ccgcgctctc gcccctcacc taccgccgcc cggtccacgc ggcccgggcc gagggggtct
     1081 ggatcgtcga ggccgacggt cgccgcctcc tcgacgccta caacaacgtc ccgaccgtgg
     1141 ggcactcgca cccgcgggtg gccgaggccg tagcccggca gtctcgccgg ctggcgacga
     1201 acgcccgcta cctctacgag ccgctgatcg agctcgccga gcgtctcgcg ggcacgatgc
     1261 cggcgggtct cgacaccgtg acgctcgtga actcggggac cgaggccaac gacctcgcct
     1321 ggcggatggc ggtggcctgg accggcgcct cgggcgggat cgtcaccgcg cacgcctatc
     1381 acggggtgag cgcggtcacg gccgacctct ccccggagac gtggggggag ccgcccgatc
     1441 acgtggagac cttcggtccc ccgggcgagg gggccggccc cgcgatcgag cggctcggcg
     1501 cccgcggcct ggagcccgcc gccctcttcc tcgacggggg cttcaccagc gacgggatcc
     1561 tcgttcccgg atctccgtac gtccgggacc tggcccggga gatccggagc gcgggtggac
     1621 tcttcgtcgc cgacgaggtc caggtcggcc acggccgcac cggcgagcac ctctggtcgt
     1681 tcgccgccga cggcgtgacg ccggacctcg tcacgatggg caagccgatg gggaacggat
     1741 tcccggtggc ggccgtcgtc acgcgccgcg aggtcgccgc taggctcggc gagtcgaccg
     1801 aggtcttctc cacgttcggc ggcaacccgg tcgccgcggc cgcggccctg gccgtgctcg
     1861 acgtgatcga ggacgagggc ctgatcgagc gcgcggcgga ggtgggcggc ctgctgctcg
     1921 aggagctccg tgcgctggcc cgccgtcacg acgaagtgcg ggacgtgcgg ggacgggggc
     1981 tcctgatcgg cgtcgagctg gcctcgcccg ccgctccggt cgccgacgcg atgcgcgagg
     2041 ccggggtgct cgtcggggtg accgggccgc gtggcgacgt cctcaaggtc cggcccccgc
     2101 tcggcttcgg cccggaccac gccacccggc tcgtggagac cctggaccgg atcctggcgc
     2161 ttcgagacgc gactcccggg gaatgatgcg ctggcggcgc agcgggcgcc gccacgaccc
     2221 gggaggtgcc ggatggacgc gctgaagctg ctgaaggaag accacgagaa ggtcaagaag
     2281 atcctcgacg agctcgagtc gacgaccgag cgaggcgtca agacccgcca ggacctgttc
     2341 gagaggctgc gtcgcgagct cgacgtccac gaggcgatcg aggaggagat cctctacccc
     2401 gccctcaagg agcaccccaa gacgaaggag ctcgcgctcg aggcctacga ggagcaccac
     2461 gtcgtggaca tggtgatgga ggagctcgag gacgtgccct tcgacgacga gagctggggc
     2521 gcgaagctca tggtcgccaa ggagaacctc gagcaccaca tcgaggagga agagggcgag
     2581 atgttccagc aggcccgtca ggtcttcgac gacgaggagc tgggcgagct gggcgaccgg
     2641 atggaggccc gcaagagcga gctgctgggc tgaggccggt cgggcccggc tatcctcggg
     2701 tccgatgcca cgcttcctca cccccaggtg gctggtcgc
//
LOCUS       DASUTZ010000069         7319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_148470, whole genome shotgun sequence.
ACCESSION   DASUTZ010000069 DASUTZ010000000
VERSION     DASUTZ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7319
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148470"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VF097_03165"
     CDS             <1..314
                     /locus_tag="VF097_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261830.1"
                     /translation="IRLFLAWLDLVGIPSEDLRFRLQIHEGADVEAALGHWAEVVDAP
                     MERFLRPILKRHNPKTRRKNVGASYVGCLLVLVPRSTDLNSRIEGWFRGIVTATGAPP
                     N"
     gene            351..423
                     /locus_tag="VF097_03170"
     tRNA            351..423
                     /locus_tag="VF097_03170"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:385..387,aa:Gln,seq:ttg)
     gene            471..806
                     /locus_tag="VF097_03175"
     CDS             471..806
                     /locus_tag="VF097_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX6261831.1"
                     /translation="MEQALRAIAEPRRRDILRLVLDRELTSGEIAGHFPEVTGPAISQ
                     HLRVLVDAGLVTVRREGPRRWYRARPEGLRGLHEYLEEFWRTSLATLTDEAETEEQRR
                     RHGEHPERS"
     gene            781..1242
                     /locus_tag="VF097_03180"
     CDS             781..1242
                     /locus_tag="VF097_03180"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX6261832.1"
                     /translation="MANIRNAAEVVEVETRIKASPETLWDFFTEPELMARWMGRANDL
                     DPRPGGVYRVEISDQVKAGGEYLELEPHSRILLTFGWEGHAVSPGSTKVEITLQPDGD
                     ETIVRLRHLDLPTDEREVHADGWRHYLERLSMAGAGEDPGPDPNASAQPGR"
     gene            1315..1530
                     /locus_tag="VF097_03185"
     CDS             1315..1530
                     /locus_tag="VF097_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261833.1"
                     /translation="MGNYSVYLHDLVKGRTRLLSRRNGGGISPDTNEQPVVSKVGLFV
                     AWTSDDEMIVGGDSNTSDDVFRWGPRK"
     gene            1575..2972
                     /gene="glmU"
                     /locus_tag="VF097_03190"
     CDS             1575..2972
                     /gene="glmU"
                     /locus_tag="VF097_03190"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX6261834.1"
                     /translation="MAKPSRRLAVVVMAAGKGKRLRSSLPKVLHPVAGKPVLWHVLRA
                     ARRLRPSRLIVVVSHGRDHVEEKVRSWDLDVTFVDQGEPLGTGHAVMVAEGAVGRADD
                     VLVLPGDNPLVTTEMLRDLMRLHRRRLPAATVQTTVLDDATGYGRVIRDGDRFERIAE
                     ERDASVAERRIHEIATSVYAFRREDLFGALPLVGRENVQGEHYLPDVLGILVDKDEEI
                     RVIGADFEGALDVNSRVALARAAAVMRRRINEELMASGVTMTDPDRTYVDAGVKVGSD
                     TTILPGVHLEGDTRIGAGCTIGPDTRIVDSRIDDEVEVTSSTVRGARIGQGSTVGPYA
                     HIRPGTLLGRRTKAGSFVEIKASRIGDGSKVPHLAYVGDATIGKDVNVGAGTITCNYD
                     GYEKHRTVIGDGAFIGSDTMLVAPVRIGRNAVTGAGSTITRDVADGELAVERSEQRNV
                     RGYAERRAKRRRGRA"
     gene            2969..3961
                     /locus_tag="VF097_03195"
     CDS             2969..3961
                     /locus_tag="VF097_03195"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0044249 - cellular biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEX6261835.1"
                     /translation="MSTIREIVTKKRMMIFAGTSHPELAQEIATHLGVELAPAKLFRF
                     ASSEIYMRSTESARGSDAFVIQTHADPVNESIMEQLIMIDALKRASAKRITAVIPYYG
                     YARQDKKSLAREPISARLVADLISVAGADRVVSVDLHSGQIQGYFDFPFDHLTALPLL
                     SDFLKDDLGLGGEEMVVVAPDAGRVKTAERLREHLDSELAFIYKRRSRHEAHKIEEMV
                     VEGDVDGRACILIDDMIDTAGTMVEGAEALQREGASRIYAAATHAVFSGKASQRLEES
                     VIQKVVVTNTLPVPAEKQLDKLTVLSIAPIIASALQAVFEDESVSEIFHGQNMP"
     gene            complement(3965..5257)
                     /locus_tag="VF097_03200"
     CDS             complement(3965..5257)
                     /locus_tag="VF097_03200"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6261836.1"
                     /translation="MSDREDAPLIASRRARAVARMRGIAVDLSPLRASRDYRLLWLGE
                     MVSHTGRHITVVALPFQIYQLTGSALAIGLIGLAQVVPLVAFSLVGGVIADRVDRRKL
                     LAATQVGLAVASVLLAGAAISGSPPLWFLYLVAGLIGALSGLDSPTRTATVPRLVGEA
                     YLPSAIALEQTLFQLSDVVGPAVGGVVLGQLGLAWAYALDAATFLVALGTVLAMRPLP
                     PDPAQREVKSVAAFREGFRYLRGRRVLQATFQVDLIAMVFGMPRALFPVLAAEVFQVG
                     PEALGLLYAAPGAGALLGALTSGWVRHVRRQGRAVLWAVAVWGLAVALFGLTDILWLA
                     LLLLGVAGAADMLSAIFRSTILQRSVPDRLRGRLSAIHIAVVVGGPRLGDLEAGVVAA
                     AVSPTFSVVSGGVATVVGVGVLAALVPAFVRYRSGDPA"
     gene            5343..6041
                     /locus_tag="VF097_03205"
     CDS             5343..6041
                     /locus_tag="VF097_03205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX6261837.1"
                     /translation="MEVKLAAEKREESGKGPARRARAAGRVPGVLYGPQLDPVNFTVD
                     LMDLWHVLHTDAGVNVLVDLKVDGEEYLTIPREVQRDILKGTLLHVDFIQIDRNKPIQ
                     VRVPIVLTGEAEGQKEGGVVEHHLWELHVEVLPLEVPESVEAEITELEMNDTLHVSDL
                     TVPEGVTVLSSEEEPVVSIVPPPVIELPEPEVEEEELLEGEEGELVEGEEAGEAAEGE
                     GEEARAEEGESEEG"
     gene            6042..6620
                     /gene="pth"
                     /locus_tag="VF097_03210"
     CDS             6042..6620
                     /gene="pth"
                     /locus_tag="VF097_03210"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011607316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX6261838.1"
                     /translation="MSAWLVVGLGNPGERYAETRHNIGGWVVERLSDRLNARLRKVRF
                     MPLLVGETTVDGERVYLLRGQTFMNVTGPPVASFIRKRKLDLDRVVVVHDEIDLPFGS
                     LKVKQGGSTAGHNGLRSLVGAIGSPDFLRVRLGVGRPPGRQDPADYVLRPFAKAERED
                     AELLAEEAADALLTLIREGLTAAQDRHNRSVR"
     gene            6628..6768
                     /locus_tag="VF097_03215"
     CDS             6628..6768
                     /locus_tag="VF097_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261839.1"
                     /translation="MSDRKPKRRLIERAILGTFMALAAFIIERRVRKALRRAEAERGG
                     DR"
     gene            6765..7166
                     /gene="msrB"
                     /locus_tag="VF097_03220"
     CDS             6765..7166
                     /gene="msrB"
                     /locus_tag="VF097_03220"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015763575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX6261840.1"
                     /translation="MSKRAVDKTDEEWRRELSPERYAVLRQQATEAPFTSELLEVKED
                     GTFRCAACGTELFSSQTKYDSGTGWPSFYEPMAEDNVELRPDTSHGMMRTEVVCATCG
                     GHLGHVFDDGPEPTGLRYCINGCALEFDSDE"
BASE COUNT         1213 a         2495 c         2619 g          992 t
ORIGIN      
        1 tgatccgctt gttcttggcc tggctggacc ttgttgggat cccgtcggag gatcttcgct
       61 tccgccttca gatccacgag ggagccgacg tcgaggcagc tctcgggcat tgggccgagg
      121 tcgtcgatgc tccgatggag cgcttcctcc gcccgatcct caagcgccac aatccgaaga
      181 cgcgacggaa gaacgtcggc gcctcatacg tggggtgtct cctggttctg gtccctcgaa
      241 gcacggacct gaacagccgg atcgagggct ggttccgggg gatcgtgacg gccacaggtg
      301 cacctcccaa ctgaatcgag cgctcctata gcgcatactt ggagacggat tccggggtgg
      361 tgtaatcagg cagcaccgtc ggctttggac cggcgagtct gggttcgaat cccagccccg
      421 gagcgaggct tcagttgaca cttaagtaag tcttcgctta gcatcgtgca atggagcagg
      481 cccttcgagc gatcgcagag ccccggcggc gcgacatcct ccggctcgtt ctcgaccgcg
      541 agctcacctc cggagagatc gccgggcact tcccagaggt cacgggcccc gcgatctccc
      601 agcatctccg cgtgctcgtg gacgccgggc tggtgaccgt gcgccgggag ggccctcgac
      661 gctggtaccg cgctcgcccc gagggcctgc gcggtctcca cgagtacctg gaggagttct
      721 ggcggacctc gctggcgacg ctgaccgacg aggccgagac cgaggagcag aggaggcggc
      781 atggcgaaca tccggaacgc agctgaggtg gtcgaagtcg agactcggat caaggcgagc
      841 cccgagaccc tctgggactt cttcaccgag ccggagctga tggcccggtg gatgggtcgg
      901 gccaacgacc tggacccgcg tccgggcggg gtctaccggg tggagatcag cgaccaggtg
      961 aaggccgggg gcgaatacct cgagctggag cctcactcga ggatcctcct gaccttcggg
     1021 tgggaggggc acgcagtgag ccccggatcc acgaaggtcg agatcacgct ccagccggac
     1081 ggagacgaga cgatcgtgcg cctgcggcac ctggacctcc ccaccgacga gcgagaggtc
     1141 cacgccgacg gctggaggca ctacctcgag cggctgtcga tggcgggagc gggagaggat
     1201 cccggccccg accccaacgc atcggcccag cccgggaggt aggggtcgtg gcggcgggcc
     1261 gggcccagcg ggcccggccc gccgccccct tgcggggacg atctgggcaa cccgatgggc
     1321 aactacagcg tctacctcca cgacctcgtc aagggcagga cgcggctcct cagccggagg
     1381 aacgggggcg ggatctcgcc cgatacgaac gagcagccgg tcgtctcgaa ggtcgggctc
     1441 ttcgtggcct ggacctccga cgacgagatg atcgtcggag gggactcgaa cacgtccgat
     1501 gacgtcttcc gatgggggcc caggaagtag cgtcgtcgga tgctcgccgg gccctcggcg
     1561 ggtaccctgt ggccgtggcc aagccctccc gtcgcctcgc cgtggtcgtg atggccgcgg
     1621 gcaaggggaa gcgtctgcgg tcgtccctgc ccaaggtcct gcatccggtc gcggggaagc
     1681 ccgtgctgtg gcacgtcctc cgggcggccc gccggctcag gccttcgcgc ctgatcgtgg
     1741 tggtcagcca cgggcgtgat cacgtcgagg agaaggtgcg atcgtgggac ctggacgtga
     1801 ccttcgtgga tcagggcgag ccgctcggca ccggccacgc cgtgatggtg gccgaggggg
     1861 cggtggggcg ggccgacgac gtgctcgtcc tccccggcga caaccccctc gtcaccaccg
     1921 agatgctgcg agacctgatg cggctccacc ggcggcgcct cccagccgcc accgtccaga
     1981 ccaccgtgct cgacgacgcc accggctacg ggcgcgtgat ccgggacggc gatcgtttcg
     2041 agcggatcgc cgaggagcga gacgcctcgg tggccgagcg ccgcatccac gagatcgcca
     2101 cgagcgtcta cgccttccgc cgggaggatc tcttcggcgc cctccccttg gtgggccgcg
     2161 agaacgtcca gggcgagcac tacctccccg acgtcctggg gatcctggtg gacaaggatg
     2221 aggagatccg ggtgatcggg gccgacttcg agggcgcgct cgatgtgaac tcgcgggtgg
     2281 ctctggcccg cgccgcggct gtgatgcgcc gccgcatcaa cgaggagctg atggcctcgg
     2341 gcgtgacgat gaccgacccc gaccgcacgt acgtggacgc gggggtgaag gtcgggtccg
     2401 acaccacgat cctccccggc gtgcatctcg agggcgacac ccggatcggc gcgggctgca
     2461 cgatcggccc cgacacccgg atcgtggact cgaggatcga cgacgaggtc gaggtcacct
     2521 cctccaccgt ccggggcgcg aggatcggcc agggatcgac ggtcgggccc tatgcccaca
     2581 tccggccggg gaccctgctc ggccgccgca ccaaggcagg ctccttcgtc gagatcaagg
     2641 cctctcggat cggggacggc tcgaaggttc cccacctcgc ctacgtgggg gacgccacga
     2701 tcgggaagga tgtcaacgtg ggggccggga cgatcacctg caactacgac ggatacgaga
     2761 agcacaggac cgtgatcggg gacggggcct tcatcggttc cgatacgatg ttggtcgcac
     2821 cggtgaggat cggccgtaac gcggtgacgg gcgcggggtc gacgatcacg cgcgacgtgg
     2881 ccgacgggga gctggcggtg gagcgatccg agcagcggaa cgtccggggg tacgcggagc
     2941 ggcgcgcgaa gcgccggagg gggagggcgt gagcaccatc cgggagatcg ttacgaagaa
     3001 gcggatgatg atcttcgccg ggacctccca tcccgagctg gcgcaggaga tcgccacgca
     3061 tctcggggtg gagctggcgc cggcgaagct cttcaggttc gccagcagcg agatctacat
     3121 gcggtcgacg gagagcgcga ggggctccga cgccttcgtg atccagaccc acgccgaccc
     3181 ggtgaacgag tcgatcatgg agcagctcat catgatcgat gccctgaaac gggcgtcggc
     3241 caagcggatc accgcggtca tcccgtacta cggctacgcg cggcaggaca agaagtcgct
     3301 ggcccgcgag ccgatctcgg cccggctcgt ggccgacctc atctcggtgg cgggcgctga
     3361 ccgcgtggtc tcggtggatc tccactcggg ccagatccag ggctacttcg acttcccctt
     3421 cgatcacctg acggcgctgc cgctgctgtc ggacttcctc aaggacgatc tgggcctcgg
     3481 tggcgaggag atggtcgtcg tcgctcccga tgccggccgg gtcaagacgg ccgagcgcct
     3541 ccgcgagcat ctcgactcgg agctggcctt catctacaag cggcgctcgc gccacgaggc
     3601 gcacaagatc gaggagatgg tggtcgaggg cgacgtggac ggacgggcct gcatcctgat
     3661 cgacgacatg atcgacacgg cggggacgat ggtcgagggg gcggaggccc tgcagcgcga
     3721 gggcgcctcg cggatctacg cggctgccac ccacgcggtc ttcagcggga aggcttccca
     3781 gcgcctcgag gagtccgtga tccagaaggt cgtcgtgacg aacacgcttc ccgtccccgc
     3841 ggagaagcag ctcgacaagc tcaccgtgct ctcgatcgcg ccgatcatcg cgtcggcgct
     3901 ccaggccgtg ttcgaggacg agtcggtctc ggagatcttc cacggccaga acatgccgta
     3961 gggctcaggc cgggtcgccc gagcggtagc gaacgaaggc ggggacgagg gcggcaagca
     4021 cgccgacgcc gaccacggtg gcgacgccgc cggagaccac cgagaacgtc ggagagaccg
     4081 ccgcggcgac cacgcccgcc tccaggtcac ccagcctcgg acccccgacg acgaccgcga
     4141 tgtggatcgc cgagagcctt ccgcggagcc ggtcgggcac cgagcgctgg aggatcgtcg
     4201 accggaagat cgcgctgagc atgtcggcgg ctccggccac ccccaggagc aggagcgcca
     4261 gccacaggat gtcggtcagc ccgaacaacg ccaccgccag cccccacacg gcgaccgccc
     4321 acaacaccgc ccgcccctgc cggcgcacgt gccgcaccca tccgctcgtg agcgcgccga
     4381 gcagcgcgcc ggctcccggg gccgcgtaga gcaggccgag cgcctccggg cccacctgga
     4441 acacctccgc cgccaggacc gggaacagcg ccctcggcat cccgaagacc atggcgatga
     4501 ggtccacctg gaaggtcgcc tgcaggaccc gtcggccgcg gaggtagcgg aacccctcgc
     4561 ggaacgccgc cacgctcttc acctcgcgct gggccgggtc gggcggcagg ggacgcatcg
     4621 cgagcacggt ccccagggcc acgaggaacg tcgcggcgtc gagcgcgtag gcccaggcca
     4681 gccccagctg tcccaggacc acgccgccca ccgcgggccc caccacgtcg ctgagctgga
     4741 acagcgtctg ctccagcgcg atcgccgagg gaaggtaggc ctcccccacg agccgcggca
     4801 ccgtggccgt ccgcgtcggg gagtccaggc cggagagcgc gccgatcagg ccggccacga
     4861 ggtagaggaa ccagagcggc ggcgatcccg agatcgccgc gcccgcgagc aggacggatg
     4921 ccacggccag gcccacctgc gtcgctgcca acagcttcct tcggtccact cggtcggcga
     4981 tcacccctcc cacgagcgag aaggccacca gcggcacgac ctgagccagg ccgatgagtc
     5041 cgatcgcaag cgccgagccc gtgagctggt agatctggaa cggcagcgcc accacggtga
     5101 tgtggcggcc cgtgtgcgag accatctcgc ccagccagag gagccggtag tcccgcgagg
     5161 cccggagcgg cgacaggtcc acggcgatcc cgcgcatccg ggcgaccgcc cgggccctcc
     5221 tcgatgcgat gagcggagcg tcctctcgat ccgacacggc gaagggatgc tagcgggtcg
     5281 gtgagggctc cgaccctccg ctacaatgtt ggctccgccg caagcagacc gcgagggaag
     5341 cgatggaagt caagctcgcc gcagagaaac gtgaggagtc cgggaagggt cccgcccgca
     5401 gggcgcgggc cgcgggccgc gtgcccgggg tcctctacgg acctcagctg gaccctgtga
     5461 acttcaccgt cgacttgatg gacctctggc acgtcctcca caccgacgcc ggggtcaacg
     5521 tcctcgtcga cctgaaggtg gacggggagg agtacctcac gatcccccgc gaggtccagc
     5581 gcgacatcct caagggaacc ctcctccacg tggacttcat ccagatcgat cgcaacaaac
     5641 cgatccaggt ccgcgtcccg atcgtcctca ccggcgaggc cgaaggccag aaggagggcg
     5701 gcgtcgtcga gcaccacctg tgggagctcc acgtcgaagt cctcccgctg gaggtgccgg
     5761 agtcggtcga ggccgagatc accgagctcg agatgaacga cacgctccac gtctcggacc
     5821 tcacggtgcc cgagggcgtc acggtgctca gctccgagga ggagcctgtg gtctcgatcg
     5881 tgccgccgcc ggtcatcgag ctgcccgagc cggaggtcga ggaggaggag ctcctcgagg
     5941 gcgaggaggg cgagctcgtg gagggcgagg aggccggaga ggccgccgag ggcgagggcg
     6001 aggaggcccg cgccgaggaa ggcgagtccg aggaaggcta ggtgtccgcc tggctcgtcg
     6061 tcgggctggg caatccgggc gagcgctacg cggagacccg tcacaacatc gggggctggg
     6121 tcgtcgagcg gctctccgat cgcctgaacg cccgcctccg gaaggtgcgg ttcatgcccc
     6181 tgctggtcgg cgagaccacc gtggacggcg agcgcgtcta cctcctgcgg ggccagacct
     6241 tcatgaacgt gaccggcccg cccgtggcca gcttcatccg caagcgcaag ctcgacctgg
     6301 accgggtggt cgtggtccac gacgagatcg acctgccctt cgggtcgctc aaggtgaagc
     6361 agggcgggtc cacggcgggc cacaacggcc tgcgctccct cgtgggcgcg atcggctcgc
     6421 ccgacttcct gcgggtgcgg ctcggcgtgg ggaggcctcc gggtcgacag gacccggccg
     6481 actacgtgtt gcggccgttc gccaaggcgg agcgcgagga tgccgagctg ctggccgagg
     6541 aggcggccga cgcgctcctc accctgatcc gcgaggggct gaccgccgcc caggatcgcc
     6601 acaaccgctc cgtgcgatag tgccggggtg agcgatcgca agcccaagcg ccgtttgatc
     6661 gagcgagcca tcctgggaac gttcatggcc ctcgccgcgt tcatcatcga gcggcgggtg
     6721 cggaaggcgc tgcgccgggc ggaggccgag cggggaggag atcgatgagc aagcgagcgg
     6781 tcgacaagac ggacgaggag tggcgccggg agctgagccc cgagcggtac gcggtcctgc
     6841 gccagcaggc caccgaggcg ccgttcacca gcgagctgct ggaggtgaag gaggacggga
     6901 cgttccgctg cgcggcctgc gggaccgagc tcttctcgtc acagacgaag tacgactcgg
     6961 gcaccggctg gccctcgttc tacgagccga tggccgagga caacgtcgag cttcggcccg
     7021 acacctcgca cgggatgatg cggaccgagg tggtctgcgc cacctgcggt gggcacctgg
     7081 gccacgtctt cgacgacggc cccgagccca cggggctccg ctactgtatc aacggctgcg
     7141 ccctggagtt cgactcggac gagtagtcag cggcggcggc gcgcgatcag gtcgtacgcc
     7201 agtcccaagg tcagcgcgcc gccgacgcct ccgagcgtca tcggcaccca tgaggccacg
     7261 tctgccgcga ccgccgtggc gctcgtggcg atgaaggccg cccccgcccc gatcgccat
//
LOCUS       DASUTZ010000070         9028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_148539, whole genome shotgun sequence.
ACCESSION   DASUTZ010000070 DASUTZ010000000
VERSION     DASUTZ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9028
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148539"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VF097_03225"
     CDS             <1..349
                     /locus_tag="VF097_03225"
                     /inference="COORDINATES: protein motif:HMM:NF044544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261841.1"
                     /translation="NARDALRYLAAMLGAREQPLAILGGVAARVRDLLRVRSLPPSMP
                     LKDVAREAGLRFDWQARRYREQAGRYDPEDLADIHRRVVEADAQLKQGGGGDVVLTML
                     VTRIANGRARVPR"
     gene            complement(351..620)
                     /gene="rpsT"
                     /locus_tag="VF097_03230"
     CDS             complement(351..620)
                     /gene="rpsT"
                     /locus_tag="VF097_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010468502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX6261842.1"
                     /translation="MANIQSQIKRNRQNERRRVRNKAVRTRLKTEAKRTLEVAEQGDL
                     EAARSAYRKTARELDRAASKGVLHPRTAARRKSRLAKRLNARQGE"
     gene            670..2490
                     /gene="lepA"
                     /locus_tag="VF097_03235"
     CDS             670..2490
                     /gene="lepA"
                     /locus_tag="VF097_03235"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX6261843.1"
                     /translation="MTSVTHPIRNFAIISHIDHGKSTLADRFLELTHAVAERQMREQF
                     LDRMDLERERGITIKAQAVRLAYELDGEPHVLNLIDTPGHVDFSYEVSRALAAAEGAI
                     LLVDAAQGIEAQTLANFHLAEQAGLTILPAVNKIDLPQADVAGAAREIAELTGVPADE
                     VLHISGKTGEGVEALLRAVVERIPPPGGDPDASTRALIFDSTYDQYRGVVAYVRVVDG
                     ILPSNARVRMVATGHESDAEEVGVFAPDPVAATALAAGEVGYLVTGVKDVRQAKVGDT
                     ITLARTEVEQLPGYREPKPMVWSGLFPEDGDEYPALRDALDRLQLNDAALVFEPETSR
                     ALGFGFRTGFLGLLHMEIVRERLEREFGLQLIATAPNVELHVIGEGGEALEVHNPSEM
                     PVPGTYEHVEEPYVLATILTPSAYLGPVMELVQDRRGTMEEMRYLSPERVEIRYLLPL
                     AEIIFDFFDQLKSGTRGYASLDYEPFGYQEGDLVRVDLLLHGEPVDAFSAVVHRDKAQ
                     AYGRRMVERLKELIPRQLFDVAVQAAIGNRIVARETVRAKRKDVTAKCYGGDITRKRK
                     LLERQKEGKRRLRRVGKVDVPHEAFIAALRVDAEARGKGR"
     gene            2487..3608
                     /gene="hemW"
                     /locus_tag="VF097_03240"
     CDS             2487..3608
                     /gene="hemW"
                     /locus_tag="VF097_03240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX6261844.1"
                     /translation="MRRPEARAGDAGIYVHIPFCLTRCGYCDFNAHSGLDHLKSPFVE
                     ALLSETELAAPSWEGVRVVSIFLGGGTPTTLAPEALGSILDRLRERFDVASDAEVTCE
                     ANPDTLDLPYLQRLRERGVTRLSVGAQSFDPAVLSALQRIHSVSSVRRAFAAARAAGF
                     DDVSLDLIYGARGETLASWERTLGEALALGPDHLSCYALTIERGTLLGREVALGLLAP
                     PEPDLQADMYEAACDRLVSAGYEHYELSNWARPRAASLHNLGYWTGRPYLGLGPGAHS
                     FREGVRRWNLRSPERYVEQLRGGMLPTGGEERPSEDERRMERLLLGLRLAEGVPASWL
                     PIDVAEDLVARGLARRYDGRVALTDPGMLLANEVVLASS"
     gene            3645..4673
                     /gene="hrcA"
                     /locus_tag="VF097_03245"
     CDS             3645..4673
                     /gene="hrcA"
                     /locus_tag="VF097_03245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX6261845.1"
                     /translation="MTIEERKAAVLRAVVRDHIRTGEPVGSKTIVSRYGLGVSAATIR
                     NELAMLEDLGYLTQPHTSAGRIPTDLGYRFYVDSLPETVALPRTLQDQIETFFGPPAG
                     DVDEVLHGTASLLSRLTGYAAVAVTPRATSSRVLRTELVPVGSSVMLLIVSDTGRVDK
                     RMLEVGHEPDERIVERASRMLSERLVGLSYDEAARRAAALIREMADEERRLVRDVASA
                     IRHMGDASGEIYLGGVANIADEAAFESPDTLREMVGALEEGSAVLEILRAASGAIDDV
                     TVRIGRENTLRAMAEASVVLAWYGPRSRPVGAIAVVGPTRMEYPKAISSTRVVAGHLS
                     DLLRGLAG"
     gene            4677..5768
                     /gene="dnaJ"
                     /locus_tag="VF097_03250"
     CDS             4677..5768
                     /gene="dnaJ"
                     /locus_tag="VF097_03250"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX6261846.1"
                     /translation="MAQTRDLYEVLGVSRNATQDEIRKAYRRLVREYHPDVSDDPHAE
                     HRIKEINLAYQTLSDPARRRQYDTYGGEGFTPEMFDFMGDVSDIFEMFFGSPFRRGRR
                     SPRRERGEDARLAIALSFEEAAFGVEKGIEVETLAPCEACDGTGREPGTEAVRCAHCG
                     GNGEVSDVRRSVFGTVMTSRICSACGGTGQQIPSPCQSCRGEGRVPRTKTITVEVPAG
                     VADGVELRIEGSGHQGRRGTPPGDLYVGLRVRPHPVFERRGQDLVAVLEVPLTQAILG
                     GEVEIETLDGPEVLKIRPGTKPGTVVRLGGRGVPNLGRRGRGDLYVELDVALPAKLSK
                     KERALVEQLAELRDEPTGKGAAPGRLRRP"
     gene            5765..6532
                     /locus_tag="VF097_03255"
     CDS             5765..6532
                     /locus_tag="VF097_03255"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEX6261847.1"
                     /translation="MSLPHFFAGAPEPGDEVVLDRDDARHATRSLRLGPGDRLTSSDG
                     RGGVAVARITEVGKDAVRALVEERRQVPPETPRLTVVLAAPKGERLTWAIQKLTEVGT
                     DRIVLAESGRSVRRWSGARAARARERAEAIAREAAKQSRRARLPDVEGPTTWAEALDG
                     AEGPVILLWEGATEGLAAVLPEEPPEALTLAVGPEGGITEEEARDAESRGAALAALGL
                     TILRTETAALAGAAIALSRYGRLGERGAPLDPGDASG"
     gene            6543..6887
                     /locus_tag="VF097_03260"
     CDS             6543..6887
                     /locus_tag="VF097_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEX6261848.1"
                     /translation="MEDCLFCRIARGEQEAEIVHAGEDVVAFRDINPQAPTHILIVPK
                     EHVESARAIRDRDGDLLVDVFHVAEHLAKAEGIERSGWRLVTNVGPDAGQSVHHLHFH
                     LLGGRPMAWPPG"
     gene            7019..7945
                     /locus_tag="VF097_03265"
     CDS             7019..7945
                     /locus_tag="VF097_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX6261849.1"
                     /translation="MVALLGERDEFLKIIESAFGSSIIVRGNEIAISGPPEESERVAV
                     LFEELLTLLERGQPLSPESVERTIDMIKRDEGRPSEVLTEVLRTARRKTIAPKTVGQQ
                     QYVDAIHRSTVTFAIGPAGTGKTYLAVAAAVRALQEKEISRIILTRPAVEAGERLGFL
                     PGTLYEKIDPYLKPLYDALYEMIEADTFQRSMERGTIEVAPLAYMRGRTLNDSFIILD
                     EAQNTSPEQMKMFLTRLGFGSRAVVNGDVTQVDLPSGQRSGLMVVEEILQGIDGIEFI
                     HLGARDVVRHKIVQDIVEAYRVFGEQQARASE"
     gene            7952..8395
                     /gene="ybeY"
                     /locus_tag="VF097_03270"
     CDS             7952..8395
                     /gene="ybeY"
                     /locus_tag="VF097_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX6261850.1"
                     /translation="MSDDPSSRPTILISNRQDLPVDEPALTRLARESLRAEGQPDVEL
                     SVSLVTRDEIADLHERYLGEPGPTDVLSFPQDDEAEDGVRLLGDVVVCPEEAAEQEGA
                     EADVGLLLVHGILHLLGYDHETDEDRAEMWARQARYAGVPMEGSS"
     gene            8401..>9028
                     /locus_tag="VF097_03275"
     CDS             8401..>9028
                     /locus_tag="VF097_03275"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HEX6261851.1"
                     /translation="MTVAVVLLVLFGSVLAMAEASISRMSRVRALALEDEGRRNASRL
                     VMIETDPPRYLNAIYLWVMLAQNGSAILVAIIADRLYGSLGITLLSVAFTLVYFIFVE
                     AMSKTFGILHSDRVALALAPVVLGLSRVLAWPTRGLIGIANILLPGKGLRTGPFVSEE
                     EIRAMAEAGSEEGAIEEEEKELIHSIFEFGDTIVREVMVPRPDMVTIGK"
BASE COUNT         1319 a         3115 c         3333 g         1261 t
ORIGIN      
        1 caacgcccgc gacgccctcc gctacctcgc cgcgatgctg ggggcccggg agcagccgct
       61 ggcgatcctg gggggcgtgg ccgcccgcgt gcgggacctg ctccgcgtgc ggagcctccc
      121 cccgagcatg ccgctgaagg atgtggctcg cgaagccggc ctgcggttcg actggcaggc
      181 gcgccgctac cgggagcagg ccggcaggta cgatcccgag gacctggccg acatccatcg
      241 gagggtggtc gaggccgatg cccagctgaa gcaggggggt ggcggcgacg tggttctgac
      301 gatgctggtg acgcggatcg cgaacgggag ggcgcgggtc ccgcgctgag ctactcgccc
      361 tgccgggcgt tcagccgctt ggccaggcga gacttgcggc gggccgccgt acgtgggtgg
      421 aggacgccct tcgaggcggc ccggtccagc tcccgggcgg tcttgcggta ggcggagcgg
      481 gcggcctcga ggtcgccctg ctcggcgacc tcgagcgtcc gcttcgcctc ggtcttcagg
      541 cgcgtgcgaa cggccttgtt gcggacgcgg cggcgctcgt tctggcggtt ccgcttgatc
      601 tgcgactgga tgttggccat cggcgggcga ttgtagcagg tcgtcggggc agagccccgg
      661 gcggctacca tgacgagcgt gacccacccg atccggaact tcgccatcat cagccacatc
      721 gaccacggga agtccacgct tgccgaccgc ttcctggagc tgacccacgc cgtggccgag
      781 cgccagatgc gcgagcagtt cctcgatcgg atggacctcg agcgcgagcg ggggatcacg
      841 atcaaggccc aggcggtgcg gctggcctac gagctcgacg gggagcccca cgtgctcaac
      901 ctgatcgaca cgcccggcca cgtggacttc tcctacgagg tctcgcgggc gctggcggcg
      961 gcggagggcg ccatcctgct cgtggacgcg gcccagggga tcgaggccca gacgctggcc
     1021 aacttccacc tggccgagca ggcgggcctc acgatcctgc cggcggtgaa caagatcgac
     1081 ctccctcagg ccgacgtggc gggcgctgcg cgggagatcg cggagctcac cggcgtcccc
     1141 gcggacgagg tgctgcacat ctcggggaag acgggggagg gcgtcgaggc ccttctgcgg
     1201 gcggtggtcg agcggatccc gccgcccggc ggggacccgg acgcctccac ccgggccctg
     1261 atcttcgact ccacctacga ccagtaccga ggggtcgtgg cctacgtgcg cgtggtggac
     1321 gggatcctgc cgtccaacgc ccgggtccgg atggtggcca ccggccacga gtccgacgcc
     1381 gaggaggtgg gcgtcttcgc cccggatccc gtggccgcga cggcgctggc cgccggagag
     1441 gtgggctacc tggtcaccgg cgtgaaagac gtccgccagg cgaaggtcgg cgacacgatc
     1501 acgctcgctc ggaccgaggt ggagcagctc cccgggtacc gggagccgaa gccgatggtc
     1561 tggtcggggc tgttccccga ggacggcgac gagtacccgg cgctccgcga cgccctcgac
     1621 cgtctccagc tgaacgacgc ggcgctggtg ttcgagccgg agacgtcccg ggcgctcggg
     1681 ttcgggttcc gcacgggctt cctggggctg ctccacatgg agatcgtccg ggagcgtctg
     1741 gagcgagagt tcgggctcca gctgatcgcc accgccccga acgtggagct gcatgtgatc
     1801 ggcgagggtg gggaggcgct cgaggtccac aacccctccg agatgcccgt cccgggaacg
     1861 tacgagcacg tcgaggagcc ctacgtcctg gccacgatcc tcaccccgag cgcgtacctc
     1921 ggccccgtga tggagctggt ccaggaccgg cgggggacga tggaggagat gcggtacctc
     1981 tcgcccgagc gcgtcgagat ccgatacctg ctcccgctgg ccgagatcat cttcgacttc
     2041 ttcgaccagc tgaagtcggg gacgcgcggc tacgcctcgc tcgactacga gcccttcggc
     2101 taccaggagg gagacctggt gcgggtggac ctcctcctgc acggcgagcc ggtggacgcc
     2161 ttctcggcgg tcgtccatcg ggacaaggcg caggcctacg gccgtcggat ggtcgagcgg
     2221 ctgaaggagc tgatcccccg gcagctcttc gacgtcgcgg tgcaggcggc gatcgggaac
     2281 cgcatcgtgg cccgggagac cgtccgcgcc aaacgcaagg acgtgacggc gaagtgctac
     2341 ggcggggaca tcacccggaa gcgcaagctg ctggagcgcc agaaggaggg aaagcggcgg
     2401 ctgcgacgcg tggggaaggt ggacgtgccg cacgaggcct tcatcgccgc gctccgcgtg
     2461 gacgccgagg cgagggggaa gggtcgatga ggcggccgga ggcgcgggct ggggacgccg
     2521 gcatctatgt ccacatcccc ttctgcctga ctcgctgcgg gtactgcgac ttcaacgccc
     2581 actcggggct ggatcacctg aagtccccct tcgtggaggc cctgctgtcg gaaacggagc
     2641 tcgctgcgcc ctcctgggag ggggtccgcg tggtctcgat cttcctcggc ggggggaccc
     2701 cgaccaccct ggcccccgag gcgctgggat cgatcctgga tcggctccgc gagcgcttcg
     2761 acgtcgcgtc cgacgccgag gtgacctgcg aggcgaaccc cgacacgttg gatcttccgt
     2821 acctgcagcg gctccgggag cgaggcgtca cccgcctctc ggtgggcgcg cagtccttcg
     2881 accccgccgt gctgtcggcg ctccagcgga tccactcggt ctcgtcggtg cgccgagctt
     2941 tcgccgccgc gcgcgccgcg gggttcgacg acgtcagcct cgacctgatc tacggcgccc
     3001 gcggggagac gctcgcgtcg tgggagcgca cgctgggcga ggcgctggcg ctaggacccg
     3061 atcacctgtc ctgctacgcg ctcacgatcg agcgggggac cctgctgggg cgcgaggtcg
     3121 ccctggggct cctcgcgccc cccgagcccg atctccaggc cgacatgtac gaggcggcct
     3181 gcgaccggct cgtgagcgcc ggctacgagc actacgagct ctcgaactgg gcccggccac
     3241 gcgcggccag cctccacaat ctcggatact ggaccggtcg tccgtacctg gggctcgggc
     3301 ccggggccca ctcgttccgg gagggcgtcc gccggtggaa cctccgctcg ccggagcgct
     3361 acgtggagca actccgcggc ggcatgcttc ccacgggggg agaggagagg cccagcgagg
     3421 atgagcggcg gatggagcgg ctcctgctgg gcctccggct ggcggagggg gtgccggcgt
     3481 cgtggctccc gatcgacgtg gccgaggatc tcgtcgctcg cggcctggcg cgtcgttacg
     3541 acggccgcgt ggctctgacc gatcccggga tgctcctggc caacgaggtc gttctggcct
     3601 cgtcctagca ctcgggccgg gtgagtgcta aaactgtagg tagcatgacc atcgaggagc
     3661 ggaaggcggc ggtcctgcga gccgtggtcc gcgaccacat ccggaccggc gagcccgtgg
     3721 ggagcaagac gatcgtgagc cggtacgggc tcggggtcag cgccgccacg atccgcaacg
     3781 agctggccat gctcgaggac ctcggctatc tgacacagcc ccacacctct gcgggtcgca
     3841 tccccacgga tctcggctac cggttctacg tcgactccct ccccgagacg gtcgccctcc
     3901 cgcgaacgct ccaggaccag atcgagacct tcttcggccc gccggcgggc gacgtagacg
     3961 aggtcctgca cggcaccgcg tccctcctct cgaggctcac gggatacgcc gccgtggccg
     4021 tcacgccccg ggcgacgagc agcagggtcc tgcgcaccga gctcgtgccg gtgggctcca
     4081 gcgtgatgct gctgatcgtc agcgacaccg gcagggtcga caagcggatg ctcgaggtgg
     4141 ggcacgagcc cgacgagcgg atcgtggagc gcgcctcgcg gatgctctcc gagcgcttgg
     4201 tcgggctctc ctacgacgag gcggcgagac gcgcggcggc gctgatccgg gagatggccg
     4261 acgaggagcg gaggctcgtc cgcgacgtgg cgtcggcgat ccggcacatg ggtgacgcct
     4321 ccggcgagat ctacctgggg ggcgtggcca acatcgccga tgaggcagcg ttcgagtctc
     4381 ccgacaccct ccgggagatg gtgggcgccc tggaggaggg ctcggcggtc ctggagatcc
     4441 tccgcgcggc ctccggcgcg atcgacgacg tcaccgtccg gatcgggcgg gagaacacgc
     4501 tccgggcgat ggcggaggcc agcgtcgtgc tggcctggta cgggccccga tcccgcccgg
     4561 tcggcgcgat cgcggtggtg ggcccgaccc gcatggagta cccgaaggcg atctcgtcga
     4621 cccgcgtggt ggccgggcac ctctcggacc tgctccgcgg gctcgcgggg taggccatgg
     4681 cgcagacgcg cgacctctac gaggtcctgg gcgtgtcccg aaacgccacc caggacgaga
     4741 tccggaaggc ctaccggcgt ctcgtgcgcg agtaccatcc cgacgtcagc gacgaccccc
     4801 atgccgagca ccgcatcaag gagatcaacc tcgcctacca gaccctctcg gacccggcgc
     4861 gtcggcgtca gtacgacacc tacgggggcg agggtttcac gcccgagatg ttcgacttca
     4921 tgggggacgt ctcggacatc ttcgagatgt tcttcggaag tcccttccgg cggggccgcc
     4981 gctccccgcg gcgcgagcgc ggggaggacg cccgactggc gatcgcgctc tccttcgagg
     5041 aggccgcctt cggcgtggag aaggggatcg aggtcgagac gctggccccg tgcgaggcgt
     5101 gcgacggcac cggccgggag ccgggcaccg aggccgtccg ctgtgcccac tgcggaggga
     5161 acggcgaggt ctcggacgtg cgacggagcg tcttcgggac ggtgatgacc tcgaggatct
     5221 gctcggcgtg cgggggcacg ggccagcaga tcccctcgcc gtgccagagc tgccggggcg
     5281 aggggcgggt gccccggacg aagacgatca ccgtggaggt gccggcgggc gtggccgacg
     5341 gcgtggagct caggatcgag ggttccgggc atcaggggag gcgagggacg ccccccggcg
     5401 acctctacgt cgggctccgc gtccggccgc accccgtgtt cgagcgaagg gggcaggatc
     5461 tcgtggccgt gctggaggtc ccgctcacgc aggcgatcct ggggggcgag gtcgagatcg
     5521 agacgctcga cggtccggag gtcctgaaga tccgcccggg aacgaagccc gggaccgtgg
     5581 tcaggctggg cggccgcggc gtcccgaacc tgggacgccg cggccgcggc gatctgtacg
     5641 tcgagctcga cgtcgccctt cccgcgaagc tctcgaagaa ggaacgggcg ctcgtcgagc
     5701 agctggccga gctccgggac gagcccaccg gcaagggcgc ggcgcccggc aggctccgcc
     5761 ggccgtgagc ctcccgcact tcttcgccgg ggcccccgag cccggcgacg aggtggtgct
     5821 ggaccgcgac gacgcccgcc atgccacccg atccctgcgc ttgggccccg gcgaccggct
     5881 cacctcgagc gacgggcggg gaggggtggc ggtggcgcgc atcaccgagg tggggaagga
     5941 cgccgtccgg gccctggtcg aggagcgacg ccaggtccca ccggagaccc cgcggctgac
     6001 ggtggtgctg gcggctccca agggggagcg gctgacgtgg gccatccaga agctcacgga
     6061 ggtggggacc gatcggatcg tgctggcgga gtccggccgc agcgtccgtc ggtggagcgg
     6121 cgcgcgcgcc gcgagggcca gggagcgcgc ggaggcgatc gcccgggagg cggccaagca
     6181 gtcgcggcgc gcgcgcctcc ccgacgtcga ggggcccacc acctgggccg aggccctgga
     6241 cggcgcggag ggaccggtga tcctcctctg ggagggcgcc accgaggggt tggccgcggt
     6301 cctgcccgaa gagccgccgg aggccctcac cctggccgtg ggccccgagg gcggcatcac
     6361 cgaggaggag gcgagggacg ccgagtcgcg gggcgccgcg ctcgccgccc tggggctgac
     6421 gatcctccgg accgagacgg ctgccctggc cggcgcggcg atcgccctct cgcgctacgg
     6481 gcggctcggc gagcgcggcg ccccgctcga cccgggtgac gcgagcggat agcatcgggc
     6541 ccatggagga ctgcctcttc tgccgcatcg cccggggcga gcaggaggcc gagatcgtcc
     6601 acgcggggga ggacgtcgtc gcgttccgcg acatcaaccc acaggccccg acccacatcc
     6661 tgatcgtccc caaggagcac gtggagtcgg cccgcgcgat cagggatcgc gacggggacc
     6721 tgctcgtgga cgtcttccac gtggcggagc acctggccaa ggccgagggg atcgaacgct
     6781 cgggctggcg gctggtgacg aacgtcgggc ccgacgcggg ccagtccgtg catcacctgc
     6841 acttccacct gctcggcggc cggcccatgg catggccccc cggctgagcg tggagggcag
     6901 ggacccgagg gtataatcgc ctcgagggtc cccggagcga ccctcttccc aaccccccag
     6961 gagcgagttg gccgagagca ccacgcaggt gaagatcttc gtccccggcg accgatcgat
     7021 ggtcgccctc ctcggcgaac gggacgagtt cctcaagatc atcgagagcg ccttcggctc
     7081 ctccatcatc gtgcgcggga acgagatcgc gatctcggga ccaccggagg agtccgagcg
     7141 cgtcgccgtc ctgttcgagg agctgctcac gctactcgag cggggccagc cgctcagccc
     7201 cgaatcggtc gagcggacga tcgacatgat caagcgggac gaggggcgcc cgtccgaggt
     7261 cctgaccgag gtcctgcgca cggcccgccg caagacgatc gccccgaaga cggtgggcca
     7321 gcagcagtac gtggacgcga tccaccgctc cacggtcacg ttcgccatcg gtcccgccgg
     7381 gaccggcaag acgtacctgg ccgtcgcggc cgccgtccgc gccctccagg agaaggagat
     7441 ctccaggatc atcctgaccc gaccggcggt ggaggcgggc gagcggctcg gcttcctccc
     7501 cgggaccctc tacgagaaga tcgacccgta cctcaagccg ctctacgatg ccctctacga
     7561 gatgatcgag gccgacacct tccagcggtc aatggagcgg ggcacgatcg aggtggcgcc
     7621 cctggcgtac atgcgcggac ggacgctgaa cgactcgttc atcatcctcg acgaggcgca
     7681 gaacacctcc cccgaacaga tgaagatgtt cctcacccgc ctcggcttcg gatcgcgcgc
     7741 ggtcgtgaac ggcgacgtga cccaggtcga cctccccagc ggccagcgct cggggctgat
     7801 ggtggtcgag gagatcctgc aggggatcga cggcatcgag ttcatccacc tcggggcccg
     7861 cgacgtcgtc cggcacaaga tcgtgcagga catcgtggag gcgtaccgtg tgttcggcga
     7921 gcagcaggct cgcgcgagcg agtgatcctc gatgagcgac gacccctcca gtagaccgac
     7981 catccttatc tccaaccgtc aggacctgcc cgtggacgag cccgcgctca cgcgcttggc
     8041 gcgggagagc ctcagggccg agggccagcc ggacgtcgag ctctcggtga gcttggtgac
     8101 gcgggacgag atcgccgacc tccacgagcg ctacctcggc gagccgggcc ccacagacgt
     8161 gctgtcgttc ccacaggacg acgaggccga ggacggcgtg cgcctgctgg gcgacgtggt
     8221 ggtctgcccc gaggaggccg ccgagcagga gggggccgag gccgacgtgg ggctgctcct
     8281 cgtccacggc atcctccacc tcctcggcta cgaccacgag accgacgagg atcgcgcgga
     8341 gatgtgggcc cggcaggccc gatacgcggg cgtcccgatg gaggggtcct cgtgatctgg
     8401 atgaccgtgg ccgtcgtgct cctcgtcctg ttcggctcgg tcctggccat ggccgaggcg
     8461 tcgatcagcc ggatgagccg cgtgcgggcc ctggcgctcg aggacgaggg gcgtcgcaac
     8521 gcctcccggc tcgtcatgat cgagaccgac ccgccccgct acctcaacgc gatctacctc
     8581 tgggtgatgc tggcccagaa cgggtcggcc atcctcgtgg cgatcatcgc cgatcgcctc
     8641 tacggcagcc tggggatcac cctgctgtcg gtggccttca ccctcgtgta cttcatcttc
     8701 gtcgaggcga tgtcgaagac cttcggcatc ctgcacagcg accgcgtggc gctggccctg
     8761 gccccggtgg tcctgggact gtccagggtg ctggcctggc ccacccgcgg cctcatcggc
     8821 atcgccaaca tcctcctccc cgggaagggc ctgcggaccg gccccttcgt ctccgaggag
     8881 gagatccggg cgatggccga ggccggcagc gaggagggcg cgatcgagga ggaggagaag
     8941 gagctgatcc actcgatctt cgagttcggc gacacgatcg tgcgcgaggt gatggtcccg
     9001 cggcccgaca tggtcacgat cgggaagg
//
LOCUS       DASUTZ010000071         3626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_279267, whole genome shotgun sequence.
ACCESSION   DASUTZ010000071 DASUTZ010000000
VERSION     DASUTZ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279267"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VF097_03280"
     CDS             <1..354
                     /locus_tag="VF097_03280"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HEX6261852.1"
                     /translation="KIANATRDLPRSEPILAALVEMGDVVRPMLRASMEAFARRDLDL
                     ARRLPSMDDPVDRLNRGMYREVAACSGDQDVLEWAVRMMVVSRQLERVGDHAVDIAEQ
                     AAFLLTGEFEEFSDG"
     gene            347..1042
                     /locus_tag="VF097_03285"
     CDS             347..1042
                     /locus_tag="VF097_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6261853.1"
                     /translation="MAEVARVLVVEDESSLAETVRYNLEREGYAVDVVQDGVRGLEAF
                     RAGAPDLVVLDLMLPELSGLDVCRAIRAESSVPIIILTARDAEADKVAGLELGADDYV
                     TKPFSMRELISRVRAGLRRVRLAGQPAHEELLRSGPVEMHVGRHEVTVRGELVRFPPK
                     EFELLEAFLRRRGRLLTRDFLIDEVWGPDYFGDTKTLDVHVKRLRAKIEEDPHHPRLL
                     VTVRGVGYKMTDG"
     gene            1035..1439
                     /locus_tag="VF097_03290"
     CDS             1035..1439
                     /locus_tag="VF097_03290"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020513924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX6261854.1"
                     /translation="MAEAPVVLFVCVHNAGRSQMAAGLLERIGGDAVRVRSAGTTPAD
                     QVHPNVVQAMREVAIDLTGVRPRRLEDEAVREADVVVTMGCGDACPFYPGKRYEDWEL
                     EDPAGKSLEVVRAIRDEIERRVRGLLADLARA"
     gene            1452..3149
                     /locus_tag="VF097_03295"
     CDS             1452..3149
                     /locus_tag="VF097_03295"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6261855.1"
                     /translation="MDRRPTLARRLVAWFALAVVGLLSLLGLVLDRAIERALLDDLTD
                     SLVTQVRTVARSLEGEEDLQPEVLRLGRGVGLRITVIRGDGAVLADSQGEPAELENHA
                     DRPEVRAALAGEVGVDSRLSESVGRPFRYVALLEGERVVRVAMPLRAVQERLGRVRAL
                     IVLGASVAAVAGVVVVYLVARRLTRPLDQMSEALARMSAGDLEVGLPAGRTAELALLG
                     ETLDRLASDLGRRIAQIQEDRRMRDSILSVMDEGVVLVGGEDVRYVNPAARKLLHGTP
                     TEVRGLAPHALQRHVREVRATGQISREEIETSDPPRIVQAEAVPVLGRDRVLLVLRDV
                     TEQRRVEAMRRDFVADASHELKTPAASIQVAAETVRDVVEEDPRAAKRFAEQLHRDAV
                     RLSTIVSDLLDLSRLEGERPEGRRQSLDRIAREEAERLEERAREAGVDLATDLGGASL
                     TGDEEGLRLLVRNLVDNAIRHTGRGGRVRLAVRSVDGAVELTVADTGPGIPRREIPRI
                     FERFYRVDRARSRETGGTGLGLAIVKHVVEQHGGRISVESELGRGSTFTVELPGRSE"
     gene            complement(3156..>3626)
                     /locus_tag="VF097_03300"
     CDS             complement(3156..>3626)
                     /locus_tag="VF097_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020123960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX6261856.1"
                     /translation="EQPLPKDHLLAHADLQQRIETPICLDETLTSPHVTDEAIRLEAC
                     RVVNLKVGRVGGIEASRRIHDLVVERRVPMWCGGMLETGIGRAVNLALAAMPGFTLPG
                     DTSSSNRYFHQDVTEPFILGRDGTMAVPDGPGIGVTPHGDLLDEIAVDRHFVRS"
BASE COUNT          483 a         1214 c         1408 g          521 t
ORIGIN      
        1 aagatcgcga acgccacgcg cgacctgccg cgcagcgagc cgatcctcgc cgccctggtc
       61 gagatggggg acgtggtccg gcccatgctc cgagcgtcga tggaggcctt cgcccggcgc
      121 gacctggacc tggcccgccg actcccctcg atggacgatc ccgtcgaccg cctgaaccgg
      181 gggatgtacc gtgaggtggc ggcctgctcg ggggaccagg acgtgctcga gtgggccgtg
      241 cggatgatgg tggtgtcgag acagctggag cgggtggggg atcacgcggt ggacatcgcc
      301 gagcaggccg cgttcctgct cacgggggag ttcgaggagt tctccgatgg ctgaggtcgc
      361 acgcgtcctc gtggtggagg acgagtcgtc gctggcggag acggtccggt acaacctgga
      421 gcgcgagggc tacgcggtgg acgtcgtgca ggacggcgtc cgcgggctcg aggccttccg
      481 ggccggggcc cccgatctcg tggtcctgga cctcatgctc cccgagctct ccgggctgga
      541 cgtgtgccgc gcgatcaggg cggagtcctc ggtcccgatc atcatcctga cggcccggga
      601 cgccgaggcc gacaaggtgg ccggcctcga gctgggcgcc gacgactacg tcacgaagcc
      661 gttctcgatg cgggagctga tctcgagggt ccgggcgggg ctccggcgcg tccgcctggc
      721 ggggcagccg gcacacgagg agctcctccg ctcgggtccc gtggagatgc acgtggggcg
      781 gcacgaggtc acggtccgcg gcgagctcgt ccgcttcccg cccaaggagt tcgagctgct
      841 ggaggccttt ctcaggaggc gggggaggct cctcacccgt gacttcctga tcgacgaggt
      901 ctggggccct gactatttcg gcgacaccaa gaccctcgac gtccacgtga agcgactccg
      961 ggcgaagatc gaggaggacc ctcatcaccc ccggctcctc gtcaccgtgc gcggcgtggg
     1021 gtacaagatg accgatggct gaggcgcccg tcgtgctgtt cgtctgtgtg cacaacgcgg
     1081 ggcggtcaca gatggccgcc ggcctgctcg agcggatcgg gggcgacgcg gttcgcgtcc
     1141 ggtcggccgg caccacgccc gccgaccagg tccatccgaa cgtcgtccag gcgatgcgag
     1201 aggtggcgat cgacctcaca ggcgtccggc cgcgccggct cgaggacgag gcggtgcgcg
     1261 aggccgacgt cgtggtcacg atgggctgcg gcgacgcctg ccccttctac cccgggaaac
     1321 ggtacgagga ctgggagctc gaggacccgg ccgggaagtc cctggaggtc gtccgcgcga
     1381 tccgcgatga gatcgagcgg cgcgtccgag gtctcctcgc cgacctggcg cgggcgtagg
     1441 gtcgcgtccc gatggatcga cgtcccaccc tcgcgcgacg gctggtcgca tggttcgccc
     1501 tggcggtggt gggcctgctc tccctgctcg gcctggtcct cgaccgcgcg atcgagcgag
     1561 cgctcctcga cgacctgacc gactcgctcg tgacgcaggt gaggacggtc gcccggagcc
     1621 tcgagggtga ggaggacctc cagcccgagg tactccggct ggggcgaggt gtcgggctca
     1681 ggatcacggt gatccgaggc gacggcgccg tcctggccga ctcccagggg gaaccggccg
     1741 agctggagaa ccacgcagat cgcccggagg ttcgcgcggc cctcgccggc gaggtcgggg
     1801 tggactcgcg gctcagcgag tcggtaggga ggcccttccg gtacgtggcc ctcctggaag
     1861 gggagcgggt cgtccgcgtg gccatgcccc tccgggccgt gcaggagcgc ctgggtcgcg
     1921 tccgcgcgct gatcgtcctg ggggcttcgg tcgccgcggt ggcgggggtg gtcgtcgtgt
     1981 acctagtggc ccggcggctg acgcggcccc tggaccagat gtccgaggcg ctggcgcgga
     2041 tgtcggccgg cgatctcgag gtcggcctcc cggccgggcg caccgccgag ctggcgctcc
     2101 tgggggagac gctggaccgg ctcgcctcgg acctcggcag gcggatcgcg cagatccagg
     2161 aggaccgccg catgcgcgat tcgatcctgt ccgtgatgga cgaaggggtg gtcctcgtgg
     2221 gaggcgagga tgtccggtac gtgaatcccg cggcccggaa gctcctgcac gggacgccga
     2281 cggaggtccg gggtctcgcg ccccacgccc tccagcgaca cgtccgggag gtgcgcgcca
     2341 cggggcagat cagcagggag gagatcgaga cctccgaccc gccccggatc gtccaggccg
     2401 aggccgtgcc ggtcctgggc cgcgaccgcg tgcttcttgt gctccgagac gtgaccgagc
     2461 agcgacgtgt cgaggcgatg cgccgggact tcgtggccga cgcctcccac gagctgaaga
     2521 cgccggcggc ctcgatccag gtggccgcgg agacggtccg ggacgtcgtg gaggaggacc
     2581 cgagggcggc gaagcgtttc gcggagcagc tccaccgcga tgcggtccgc ctctcgacga
     2641 tcgtctcgga cctgctcgac ctctcgcgcc tggaggggga gcggcccgag gggcgccgtc
     2701 agtcgctgga ccgcatcgcc cgcgaggagg cggagcggct cgaggagcgg gcccgggagg
     2761 ccggcgtgga tctggccacc gacctgggcg gcgcctcgct gacgggcgac gaggaggggc
     2821 tgcggctcct ggtccggaac ctcgtggaca acgcgatcag gcacacgggc cgcgggggcc
     2881 gggtgcggct cgccgtccgc tccgtcgacg gcgccgtgga gctgacggtg gccgacaccg
     2941 gcccggggat cccgcggcgg gagatcccca ggatcttcga gcgcttctat cgtgtcgacc
     3001 gcgcccgctc ccgggagacg ggaggcaccg ggctggggct ggccatcgtg aagcacgtgg
     3061 tggagcagca cggcggtcgc atcagcgtgg agagcgagct gggacggggg tcgaccttca
     3121 cggtcgagct ccccggccgt tccgagtagg tcccgtcaag accggacgaa gtgccggtcc
     3181 acggcgatct cgtcgagaag atccccgtgt ggggtgacgc cgatgcccgg gccgtcgggc
     3241 acggccatcg tgccgtcgcg ccccaggatg aagggctcgg tgacgtcctg gtggaagtag
     3301 cggttcgagc tgctcgtgtc gcccggcagg gtgaaccccg gcatcgccgc cagcgccagg
     3361 ttcaccgcgc ggccgatgcc ggtctcgagc atgcccccgc accacatggg gacccggcgc
     3421 tcgaccacga ggtcgtggat ccgccgggag gcctcgatcc cccccacgcg ccccaccttg
     3481 aggttgacca cgcggcacgc ctcgagccgg atcgcctcgt cggtcacgtg gggcgaggtc
     3541 agggtctcgt cgaggcagat cggcgtctcg atgcgctgct ggaggtcggc gtgggccagg
     3601 aggtggtcct tggggagggg ctgctc
//
LOCUS       DASUTZ010000072         2099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1574989, whole genome shotgun sequence.
ACCESSION   DASUTZ010000072 DASUTZ010000000
VERSION     DASUTZ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574989"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VF097_03305"
     CDS             <1..442
                     /locus_tag="VF097_03305"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX6261857.1"
                     /translation="GVSYEVMTHERTYTSIEEARALGLEADEVAKTLLLDLESGHAAA
                     VIPASRRLDMHLVKEAVGDRHVRLASEDEIERDLGGFELGSIPPIPSLLGVPVYVDPD
                     LLAHETIVFASGTQTETVQARTEDVFRDERYTPAPLSREPEAES"
     gene            complement(500..1321)
                     /locus_tag="VF097_03310"
     CDS             complement(500..1321)
                     /locus_tag="VF097_03310"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX6261858.1"
                     /translation="MPGNDRRAEGERPTPAAGWEGSPARFIAGVFAVSAPLWLLGGRR
                     LPVPGMRLPLSAVQLVAPLAVSCALAYREGGPRRVRRLLRRALDAKRIEKRIWYPVLV
                     GLLPAVMLATYAFMRMSGIDLRGQAAPITAIPALLLIFLLTGAMEEIGWTGYATDPLQ
                     RRMGVLRAGLVLGVVWALWHLVPWIQGGNPPAWVVAQSFRSLALRVLIVCLYNATGKS
                     VFAATLLHAFDNVGVALFPGYDSPQAPAISGVLLGAAAGLAMLLRPGRPGVSARR"
     gene            complement(1380..>2099)
                     /locus_tag="VF097_03315"
     CDS             complement(1380..>2099)
                     /locus_tag="VF097_03315"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6261859.1"
                     /translation="AEALLGDAKRVVELDFGQFDRAEDVNTLLGAPPAYIGFGEHLPL
                     HDLARMPWSVVVCKNVDGCHPQVREVLAQALSDGFLTERSGKRVYLSDAMVVLTAPSE
                     GAGRLSPGFLSEERTSSMERVLGARLVEQVDVVSTTRPVQQTEGVGWLEGTLLEGLLE
                     QYREDGLDVEWGDSFLAWVRDQQPSLPSRLLLTRLVERRLGEALIPHLPAPGARAAVL
                     VEAEDRELKVTERAGRRRGDR"
BASE COUNT          337 a          750 c          742 g          270 t
ORIGIN      
        1 gggggtcagc tacgaggtga tgacccacga gcgcacgtac acctcgatcg aggaggcccg
       61 cgcgctgggc ctggaggccg acgaggtcgc caagacgctc ctcctcgacc tggagtcggg
      121 ccacgcggcc gcggtcatcc ccgcctccag gcgcctggac atgcatctgg tgaaggaggc
      181 ggttggcgac cgccacgtcc ggctcgccag cgaggacgag atcgagcggg acctcggggg
      241 cttcgagctc ggatcgatcc ccccgatccc ctccctactc ggcgtgcccg tctatgtcga
      301 tcccgatctg ctggcccacg agacgatcgt cttcgcgagc ggcacccaga ccgagacggt
      361 ccaggcacgc accgaggacg tcttcaggga cgagcgctac acgcccgccc cgctgtcgcg
      421 agagccggag gccgagagct agggtccacc cctgggacgc gctggagacc ctgggggcgg
      481 aaggccagga tgatctccat cacctgcgtg ccgagacgcc gggccggccc ggacggagaa
      541 gcatcgccag gcccgccgct gcgcccagca ggacgccgga gatggcggga gcctgcggcg
      601 agtcgtagcc ggggaacagc gcgacgccga cgttgtcgaa cgcgtggagc agggtggccg
      661 cgaagacgct cttccccgtt gcgttgtaga ggcagacgat cagcactcgc agcgccaggc
      721 tcctgaagga ttgggcgacc acccacgcag gagggttgcc tccctggatc cagggcacca
      781 ggtgccagag ggcccagacc actcctagga cgagcccggc ccggagcacc cccatccggc
      841 gctggagcgg atcggtggcg taccccgtcc atccgatctc ctccatcgcc ccggtgagca
      901 gaaagatgag gaggagcgcc gggatcgcgg tgatcggcgc ggcctggccc cggaggtcga
      961 tcccggacat gcgcatgaag gcgtacgtgg cgagcatgac ggcggggagg aggcctacga
     1021 gcaccgggta ccagatcctc ttctcgatcc tcttggcatc gagggccctc cgcagcagtc
     1081 tcctcacccg gcgcggtccc ccctctcgat aggcgagcgc gcacgacacg gcgaggggcg
     1141 cgacgagctg gacggcgctg agcggcagcc gcatgcccgg gacggggagc cgtcgcccac
     1201 cgaggagcca cagaggggcg gagacggcga aaacccccgc gatgaagcgc gccggggacc
     1261 cctcccaacc cgccgcgggg gtgggccgct cgccttctgc gcgccggtcg ttcccaggca
     1321 cggcccgagg ctagtacgga gccgcaggtg ggggcagcga gcggaccaag aagcggtcct
     1381 tatctatccc ctcgtctccg tccggcccgc tcggtcacct tcagctcccg gtcctccgcc
     1441 tcgacgagga cggcggcccg cgccccgggg gcggggaggt gcgggatcag ggcctctccc
     1501 agacgccgct cgaccagccg ggtgagcagc agcctcgacg ggagcgaagg ctgctggtcc
     1561 ctgacccagg cgaggaagga gtctccccac tccacgtcca ggccgtcctc gcggtactgc
     1621 tccaagagcc cctcgaggag cgttccctcg agccacccca cgccctcggt ctgctggacc
     1681 ggcctggtcg tgctcacgac gtcgacctgc tcgacgagcc tggcgccgag gacccgctcc
     1741 atcgaggagg tcctttcctc ggaaaggaag cccggggaca ggcgtcccgc gccttcggag
     1801 ggcgcggtca ggaccaccat ggcgtcgctg aggtacacac gcttcccgct ccgctcggtc
     1861 aggaacccgt cggagagcgc ctgggccagg acctcgcgga cctgggggtg gcagccgtcc
     1921 acgttcttgc acacgacgac gctccagggc atccgggcca ggtcgtgcag cgggaggtgc
     1981 tcgccgaacc cgatgtaggc gggcggggcg ccaaggagcg tgttcacgtc ctcggctcgg
     2041 tcgaattgcc cgaagtccaa ctcaacgacg cgcttcgcgt cgccgaggag ggcctccgc
//
LOCUS       DASUTZ010000073         4160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_797004, whole genome shotgun sequence.
ACCESSION   DASUTZ010000073 DASUTZ010000000
VERSION     DASUTZ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797004"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="VF097_03320"
     CDS             complement(<1..806)
                     /locus_tag="VF097_03320"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6261860.1"
                     /translation="MDDVVLLTGGTGFLGTEIARRLLRRPEARIVVLVHASGEDEGLL
                     AAKRAWSYAPDLEEGLAAGRVEIVAGDVRSADLGLEPSVRSDLIRRVTRIVHAAADLR
                     IDVVPADLRPTNVRGVANVLAFALACHADHGLVRLSHVSTAYVAGGRTGEVPEDALDD
                     RFGFENAYELTKYEGERLVRDAMKHLPISVFRPAMIVGDSRTGEVRTFNSFYVPLRRY
                     LTGRVRVVPASPRLRVNIVPVDHVADAVVRLTFEPAAEGSTFHLTAPAEHL"
     gene            complement(794..2074)
                     /locus_tag="VF097_03325"
     CDS             complement(794..2074)
                     /locus_tag="VF097_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEX6261861.1"
                     /translation="MGRHDQQTLAEGVRRDRDPRVLLVGYNGANNTGAEALLLVDIAD
                     IQAVLGPDARLTIPALEPRNLARYVGGLPGVDVVRMPMIFFPTIRRLVREHDLILLVE
                     GATYMDASASALLWYFMWATRCAATAEKPCLAYAVDAGELKPLNRRLVRRIAPRTDLI
                     IVRSAAAGERLRSWGVDTRIEVTADNAFRFEPRAEDARWVEDAWPEAGPHPVGIAAVD
                     YNVWPVVVRPWGRRADCYRWPYYYSRSPERRAASAALAEGFARLADGIIERHDRSVAL
                     ICMEQLDETLAERIHERMVHSDRARMFSSRDHDASKMTVLLRSLGALVTSRYHASVLS
                     LPAGVPQVAVRQDLRLQTLYEEMGLRDLCVAPGPHMFDVVRSAVDRLLADDGSLPARL
                     RREHARHLEAARRNPELLAAFVAEHGWRSPAWTT"
     gene            2353..3711
                     /locus_tag="VF097_03330"
     CDS             2353..3711
                     /locus_tag="VF097_03330"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6261862.1"
                     /translation="MVALLHGLGVGIAVALLIWTALLFVPFRWRPVGLYLFLPKVAAG
                     AFTPFIAAAALLLALTGVLRGSWWMGVPAALAALGASFVIVRLGLVRADLAAALGPGW
                     ADRIPPARRDRMVGRMWRGPLPGAPEPRLREDVVFATVPGTGRELLCDVWEPPEEVPP
                     SGLAVVYLHGGAYYILDKDAGTRPLFRHLASQGHVIVDVAYRLFPETDVPGMVTDAMR
                     AVAWVKEHAALLSIQPDRIVLAGGSSGGHLALLATYAHGDPVLRPADLSGSDLDVCGV
                     ASLYGQVDLAAMYEHTSQGKVCRPDDPQPDWDTPPPRWALRLFGEDAVRLRLHHMEAG
                     RCDWLVGGTPGEVPERYEPLSVLTHIRRDCPPTLLAHGLHDEMAPVAGARALHRRLRE
                     AGVPVSAVYLPHTDHMFDLIATRWSPQARAAIHALERFLSALATGPARAWEPIGAREA
                     PR"
     gene            3732..>4160
                     /locus_tag="VF097_03335"
     CDS             3732..>4160
                     /locus_tag="VF097_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX6261863.1"
                     /translation="MTKSRTVEEMAAPLGSIESGRLVFTRGASHLELRVDAQMEDLYR
                     ARFEGKVPEIRVTDGTVSVRYRSSLHPPSGEITLSGRIPWALEARWGMSDVTADLQEL
                     ELTSLEISGGASKVEVRMPHPKVAVPIRIGGGASDITVIRP"
BASE COUNT          582 a         1484 c         1491 g          603 t
ORIGIN      
        1 agatgctccg cgggagcggt gaggtggaag gtggaccctt cggcggccgg ctcgaacgtc
       61 agacgcacga cggcgtccgc cacgtggtcg accgggacga tgttgacgcg caggcggggg
      121 ctcgccggca ccacccgcac acgacccgtc agatagcggc gcagggggac gtagaagctg
      181 ttgaacgtgc ggacctcccc cgttcgggaa tcgcccacga tcatggcggg cctgaagacg
      241 gagatcggca ggtgcttcat cgcgtctcgg accaggcgct ccccctcgta cttcgtcagc
      301 tcgtacgcgt tctcgaagcc gaagcgatcg tcgagcgcgt cctcggggac ctcgccggtc
      361 ctcccgccgg ccacgtaggc cgtcgagacg tgcgagaggc gcaccagccc gtggtcggcg
      421 tggcaggcca gggcgaaggc gagcacgttc gccacgcccc ggacgttcgt tgggcggaga
      481 tcggcgggga cgacgtcgat cctgaggtcc gccgcggcgt ggacgatgcg ggtgactcgt
      541 cggatgagat cactccggac gctgggctcg agccccagat cggcggagcg cacgtcgccc
      601 gccacgatct ccacccgacc cgccgccagc ccctcctcga gatccggagc gtaggaccag
      661 gccctcttcg cggccagcag cccctcgtcc tcgccggagg cgtggaccag gacgacgatc
      721 ctcgcctcgg gccgccgcag cagcctgcgg gcgatctcgg tccccaaaaa ccccgttccg
      781 cccgtcagca ggactacgtc gtccatgccg gcgacctcca cccgtgctcg gcgacgaagg
      841 ccgccagcag ctcggggttc cggcgggccg cctcgagatg gcgagcgtgc tcccgacgga
      901 gacgggcggg cagcgacccg tcatcggcca gcagccgatc gaccgcggag cggaccacat
      961 cgaacatgtg ggggccggga gcgacgcaca ggtcccggag ccccatctcc tcgtacagcg
     1021 tctgcagccg cagatcctgc cgcaccgcca cctggggcac gccggccggg agcgagagga
     1081 cggaggcgtg atatcgggac gtgacgagcg cgccgaggct ccggagcagc accgtcatct
     1141 tcgaggcgtc gtggtcgcgc gaggagaaca tccgggcccg atccgagtgg accatgcgct
     1201 catggatgcg ctctgccagc gtctcgtcga gctgctccat gcagatcagc gcgaccgagc
     1261 gatcgtgccg ttcgatgatc ccgtcggcga gccgagcgaa gccctcggcg agggcagcgc
     1321 tcgcggcgcg gcgctccggg gatcgcgagt agtagtaggg ccagcggtag cagtcggctc
     1381 gtcgccccca cggccggacg accacgggcc acacgttgta gtcgacggcc gcgatgccga
     1441 cggggtgcgg cccggcctcg ggccaggcgt cctcgaccca ccgcgcatcc tcagccctgg
     1501 gctcgaaccg gaacgcgttg tccgcggtga cctcgatccg tgtgtcgacg ccccagcttc
     1561 gcagccgctc cccggccgcg gccgagcgga cgatgatcag gtccgtccgc ggtgcgatgc
     1621 ggcgaacgag ccgccggttc agcggcttca gctccccggc atcgaccgcg taggcgaggc
     1681 atggcttctc ggccgtggcc gcgcacctcg tcgcccacat gaagtaccag aggagcgccg
     1741 aggcgctcgc gtccatgtag gtggcgccct ccaccaggag gatgaggtcg tgctcgcgca
     1801 cgagccttcg gatggtcggg aagaagatca tcggcatccg gacgacgtcg acgcccggca
     1861 gaccgccgac gtagcgagcg aggttccgag gctcgagcgc cgggatcgtc agccgggcgt
     1921 cgggcccaag caccgcctgg atgtccgcga tgtccacgag gaggagggcc tccgctcccg
     1981 tgttgttggc gccgttgtag ccgaccagca gcaccctcgg atcacggtcc cggcggaccc
     2041 cttccgccag cgtctgctga tcgtgccggc ccatcgttcc acctctcgga tgcagtctcc
     2101 ctcggccgta cggcccccct taggggcgat gggcccggca accgtgggtc gctcgggcgg
     2161 cggccgcggc atggcgcaga gcggccgtcg ggagtccgct cagggaatgg gagtcctccg
     2221 gcccgcgacg agccctgcgt tcgaccctag gccgtgaggc cctgagggag catgctgggg
     2281 atcacccgag agatgccggg cctcgcctgg gcgctcgcgc gccctcaggg cgggccggga
     2341 ggagggtgcg agatggtcgc cctgttgcac gggctgggcg tcgggatcgc ggtcgccctc
     2401 ctgatctgga cggcactgct cttcgtgccc ttccgttggc ggcccgtggg cctgtacctc
     2461 ttcctcccca aggtcgccgc cggcgccttc acaccgttca tcgccgccgc cgcgctcctc
     2521 ctcgccttga cgggggtgct gcgagggtcc tggtggatgg gggtgccggc cgcgctcgcc
     2581 gcgctcgggg cctccttcgt gatcgtgcgc ttgggcctgg ttcgcgccga cctcgccgcc
     2641 gcgctcggcc ccggctgggc cgatcggatc cctccggcgc gtcgggaccg gatggtgggc
     2701 cggatgtggc gtggaccgct gcccggcgcc cccgagcccc gccttcggga agacgtcgtc
     2761 ttcgccaccg tgccgggcac gggcagagag ctcctctgcg acgtgtggga gccgcccgag
     2821 gaggtgccgc cctcggggct ggcggtcgtc tacctgcacg gcggcgccta ctacatcctg
     2881 gataaggacg cgggcacccg gccgctcttc cgccacctgg cctcgcaggg ccacgtgatc
     2941 gtggacgtgg cctaccgact gttccccgag acggacgtcc ccgggatggt gaccgacgcg
     3001 atgcgtgccg tggcctgggt gaaagagcac gccgcccttc tgagcatcca gccggaccgg
     3061 atcgtgctcg ccggcggatc gtcgggcggc cacctcgcgc tgttggccac ctacgcgcac
     3121 ggggacccgg tcctcaggcc ggcggatctc tccggatccg atctggacgt ctgcggcgtc
     3181 gcgtcactct acgggcaggt ggatctggcg gcgatgtacg agcacacgtc gcaggggaag
     3241 gtctgccgcc ccgacgatcc gcagcccgac tgggacacac cgccgccccg gtgggcgctc
     3301 cgcctgttcg gagaggacgc cgtccgtctg cgcctccacc acatggaggc cggtcgatgc
     3361 gactggctgg tcggcgggac gcccggcgag gtccccgaac gttacgagcc cctgtcggtg
     3421 ctcacccaca tccgccgaga ctgcccgccc acgctgcttg cccacgggct gcacgacgag
     3481 atggcgccgg tcgccggggc ccgggcgctt caccggcgcc tccgggaggc tggcgtgccc
     3541 gtgtccgctg tctacctgcc gcacaccgat cacatgttcg acctgatcgc cacccgctgg
     3601 tccccccagg cccgggcggc gatccacgcg ctggagcgct tcctgtccgc cctggccacg
     3661 ggcccggcgc gagcgtggga gcccatcggg gcccgtgagg ccccgaggtg acgtgaaagg
     3721 agatggagaa gatgacgaag agcaggaccg tcgaggagat ggccgccccg ctgggctcga
     3781 tcgaatcggg tcgcctcgtg ttcacgcggg gcgcttcaca cctggagctg agggtggacg
     3841 ctcagatgga ggatctctac cgagcccgtt tcgaggggaa ggtgcccgag attcgggtca
     3901 ccgatgggac cgtgtccgta cgctaccgtt ccagcctcca tccgccgagc ggcgagatca
     3961 cgctctccgg gaggatccca tgggcgctcg aggccaggtg ggggatgtcg gacgtcacgg
     4021 ccgacctgca ggagctcgag ctcacgagcc tggagatctc aggaggagcc tcgaaggtcg
     4081 aggtgcggat gcctcacccg aaggtcgccg tcccgatccg gatcggcggt ggcgccagcg
     4141 acatcacggt gatccggccg
//
LOCUS       DASUTZ010000074        20674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1834522, whole genome shotgun sequence.
ACCESSION   DASUTZ010000074 DASUTZ010000000
VERSION     DASUTZ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 20674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20674
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1834522"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="VF097_03340"
     CDS             complement(<1..1026)
                     /locus_tag="VF097_03340"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6261864.1"
                     /translation="MSPEERPAREERAGILGRGPFRTLLAGQTVSSLGDWVGTLAFIA
                     AAYELTGSPAAVGGVLVIRLLPPLLAAPLGGVLADRFDRRRLMVLTNLGMAVLISLAP
                     FAGLALLYAIAFASEILAMLFLPARDSAVPDLVPPDSLPQANGFVLATSYGAIPFAAA
                     LFSGLRLLTPLPEQPLAVPFLFDALTFVVAAVMIARIPIPKRRIRTDLRVVEGLGEAF
                     RFARRSPTVRGLAAGMGVAMLGGGVLFAVGIAYVRETLGGGDVEFGYLVSLWGLGMAA
                     GLGSARLFVRRGEALAFRVAVATAGGVLIAMALLPFLWLAFVGALVFGASFALAIMLA
                     MTLIQRLD"
     gene            complement(1029..1448)
                     /locus_tag="VF097_03345"
     CDS             complement(1029..1448)
                     /locus_tag="VF097_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6261865.1"
                     /translation="MTRSAERALAEVPLFEGLTRDQIRSVLDATVEVTCDAGQSMVDE
                     GMLAIDFYLILDGEAEVVSGGRHIASLRPGDHFGELAVIDGEPRSATVRATTRLRALR
                     LVADDFHRLLRTTPELAHRILVEAVRRLRSSPPPPTA"
     gene            1541..2368
                     /locus_tag="VF097_03350"
     CDS             1541..2368
                     /locus_tag="VF097_03350"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX6261866.1"
                     /translation="MVGVVTDSAAGLPPEMAARMGIEVVPLYLSVGGRSLRDGELPDL
                     YERLEREPGSVTTSMPTPGDFEEAFARRPEEELTCVTLAASVSGLHGAARMAAEAIRK
                     RVEVIDSGGASMGEGWVALAAARAAAAGGSLEEVAAAARAAAERTRLVATIDTMEHLR
                     RSGRVGWIAALAATGLQLKPVFEMARGEIRPVAKPRTRRRALDRVAAEALRDLEGGRA
                     RVAAIHARAEEEARDLLERVRPNTEVLEELVVEFTAVMGAHTGPGVVGLAYHLEASP"
     gene            2365..3000
                     /locus_tag="VF097_03355"
     CDS             2365..3000
                     /locus_tag="VF097_03355"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX6261867.1"
                     /translation="MTEETWFGPAWVFLAYLVGSIPSTYLVARVRGAIEVLRLASRKA
                     SELDAHILLTAHAGPRWSGVAGVADVVKALLFALASGWVGFEPPWQAAAGVALVAGYC
                     WPPYASRFAGRGLAVAAGVSLALLPLPTLVFGLLTLGGVALGATGLWSTIGLLSIPLT
                     AWLLDAPGALVAMAAAILALILLRRIEGLGRAEGVPLARAALRRAVFDRTS"
     gene            3031..4272
                     /locus_tag="VF097_03360"
     CDS             3031..4272
                     /locus_tag="VF097_03360"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX6261868.1"
                     /translation="MGIVVQKYGGTSVADLDRIRSVAGRVIEAKEAGDEVCVVVSAMG
                     DTTDELLGMAHRITPVPHGRELDLLLTAGERIAMSLLAITINAEGYPAASYTGSQAGI
                     ITDNRHGKAKIIDIRPGRVREALDRGDVVIVAGFQGVSTAHDVTTLGRGGSDTTAVAL
                     AAALGAEVCEINTDVGGVSTADPRIVPEARKLHAVSYEEMLEMAATGAKVLELRAVEY
                     ARNSGIKLHVRSSFDSEGGTWIAEEDERMEQAIISGVTHDTSQAKVTLVDVPDRPGIA
                     ATIFKAVADEGLNVDMIVQNVSREGITDLSFTIPKDDLPHAGKAIGRVAEEIGVAYDT
                     DADIAKISLIGAGMKTHPGVAARMFDALARAGINLEMISTSAIRISCVIRASDVEQAV
                     RVLHDEFRLSEEDVIREHEGA"
     gene            complement(4269..5192)
                     /locus_tag="VF097_03365"
     CDS             complement(4269..5192)
                     /locus_tag="VF097_03365"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6261869.1"
                     /translation="MAKLIQTSHGTRSEAFGPVEWGLLASIALMWGSSFLWIAIGLEG
                     FEPGLIAFLRVALGASFLGLMSRARRGIEREDWPRVALLGLVWVAIPFTLFPVAQQWI
                     DSSTAGMINGAMPLFTAVVATLLLRTMPGARQLIGLAVGFAGVVLVSLPAAGEGGSRL
                     LGIGLVLLATILYGLAANLAVPLQQKYGALPVVWRAQLASVVAMLPYGLISIPGSSFR
                     LSSLGAVLILGFLSTGVALVAMAVLVGRAGATRGALAIYFIPVVATILGVVFRDETVE
                     PVVFAGIALIIAGAGLASRRERRREPVPAPA"
     gene            5234..5614
                     /locus_tag="VF097_03370"
     CDS             5234..5614
                     /locus_tag="VF097_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013806925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6261870.1"
                     /translation="MRTVDYVIRYVEDLDRSVAFYRDVLGLEHRFTDEAYAEFSAGGT
                     KLGFFPRSSLPDLLGREAPGGGPPDGEIVLLVEDVDAEAQRLRDAGVEILTGPVDRPW
                     GHRTIHLLDPDGLVVEVAQEIERA"
     gene            complement(5625..5915)
                     /locus_tag="VF097_03375"
     CDS             complement(5625..5915)
                     /locus_tag="VF097_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012939433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HEX6261871.1"
                     /translation="MRISELQDLMRRTYLERDLRRGADGTFRWLVEEIGEVARALRRD
                     DPEAIRDEVADALAWLTSLANVANVDLEEAMSRYADGCPKCGEIPCACPFRA"
     gene            complement(5923..6426)
                     /locus_tag="VF097_03380"
     CDS             complement(5923..6426)
                     /locus_tag="VF097_03380"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6261872.1"
                     /translation="MSLPQTTKGFALGPDEGEALWFNGGLGILKATADQTEGRYTAYE
                     LRIPKGFGPPLHAHEHEDELFIVLSGEVRLQHGDEVIEGVPGTFAYTPKGVGHAFLAD
                     SEEARLLLLFGPAGVEGFFRDAGGPARSLQLPPADEPLRDREELVEIMRRYGQMVLGP
                     PLPPKDV"
     gene            complement(6470..6868)
                     /locus_tag="VF097_03385"
     CDS             complement(6470..6868)
                     /locus_tag="VF097_03385"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HEX6261873.1"
                     /translation="MIDHNYAALATAVASAFVISTAYYIVLGRQYAEARGLSPEAAEA
                     RPPAWKILVELVRSLTVAAVVAGLAGLLEVPDLAGATGLALALWMAFPVVLLVGAVIW
                     DEARPKVAAIHAGHWLVKLVVVASIVGLWR"
     gene            complement(7098..7171)
                     /locus_tag="VF097_03390"
     tRNA            complement(7098..7171)
                     /locus_tag="VF097_03390"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7135..7137),aa:Pro,seq:cgg)
     gene            complement(7217..8632)
                     /locus_tag="VF097_03395"
     CDS             complement(7217..8632)
                     /locus_tag="VF097_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEX6261874.1"
                     /translation="MLAPEVDSQEGVAATANDDLLPQDVLFQLDEIGSADILVGIPCF
                     NNEETIGGVIRAVEAGLRKHFPERRSVICVSDGGSADRTVAVAQSAEIGEDWKLLLVP
                     GDSPVPKRIAFPYSGLSGKGSAFRSIFEVAARLGVAACGVVDSDLRSITPYWLDRILS
                     PIVHYGYEYVAPIYARHKFDGTITNCIAFPVTTALYGVRIRQPIGGEFGFSGELAKVY
                     AAKNVWESDVARFGIDIWMTTVALVEGAKMAQGILGAKLHDAKDPGKDLGPMFRQVVG
                     SLFALAGRYRNDWWETDTATSPPIFGFPAAYSAEPIKVSVPRLTWKFVEGYVKHQALW
                     TQVLSEESLHGVQTAVGDAAETTRGLVLDLDTWTKIVYDFLIAYNGRRVDPGTLIDSM
                     IPLYFARTATFIQEVADADLHEAEDRIQANADAALALKPYLKRRWREEGIPERDLSEQ
                     EVPMEGESPEGMVGALEQQNK"
     gene            8673..8867
                     /locus_tag="VF097_03400"
     CDS             8673..8867
                     /locus_tag="VF097_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261875.1"
                     /translation="MEPRDCTWCGSARSVEHDVCQVCLMRFPELKNADEVVVILDERE
                     LQIHSVPAEEEEAARTAVSE"
     gene            8878..9921
                     /locus_tag="VF097_03405"
     CDS             8878..9921
                     /locus_tag="VF097_03405"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6261876.1"
                     /translation="MRIAQVSPPWVAVPPKGYGGIEWVVSLLADGLAERGHDVTLFAT
                     GDSQTKARLEYVFEAAPGSTAINSIWHDTVHQMLVHRDFGRFDVLHHHTPFSALVAAM
                     CHDVPVVHTLHGDFTPQFRLLYEQVADRLTFVAISQSQRAAMPELRYGGVVYNGIDLD
                     LYPFRSEKEGFLCFIGRANPDKGAHRAVLAARAAGMPLVMAVKMAHPVEREHWTREVE
                     PLLGPDVTVLGEISVEEKADLMSRARAVLFPIDWPEPFGLVMTEAMACGTPVIATPRG
                     AVPEVITDGRTGFLVSVEGYPEEAAHAIGRLGEIDPEACRSHVADRFSKESMVEGYER
                     VFERVVEGGRSGD"
     gene            9914..10414
                     /locus_tag="VF097_03410"
     CDS             9914..10414
                     /locus_tag="VF097_03410"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX6261877.1"
                     /translation="MTDVKASIEKAVAYLTEHPDDARYTDSAATARLGDALRVEVEGP
                     AGERLTTDMPGGVGGRGELPTPGWLFRAAVASCVASTVGMEAAREGVDVGDVEVLVDS
                     ESDDRGILGIDDGVPAGPLSMRIRISLRGGEASDRAIEVAERGAARCPVCDATKRAVE
                     VSVEIG"
     gene            complement(10416..11282)
                     /locus_tag="VF097_03415"
     CDS             complement(10416..11282)
                     /locus_tag="VF097_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX6261878.1"
                     /translation="MLWWILLGLLAAGLLVAAYAILIERRWYRLARYRLDILPGGGPL
                     TILHLSDLHMVDGEAHKRRFLESLPRADVTIITGDLLGEPKAVEYVVESLRPLRGRLG
                     SMFVLGSNDVYVPRPLNPLRYFVPHEKRRRRRGRRGRAPDLVAQLEADGWVHLRNLRR
                     GGSLDGVPMEILGLDDPHIHRGNVSLSPRRGTGRFGLAVVHSPDPAPELAALGWDLIV
                     TGHTHGGQVRLPWGRAIVTNSSIPRRMASGLHRIGRTFMHVSPGLGTSKYAPFRLFCR
                     PEATMLELRSAG"
     gene            11295..11744
                     /locus_tag="VF097_03420"
     CDS             11295..11744
                     /locus_tag="VF097_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018397810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX6261879.1"
                     /translation="MGALKDRLSMELREAIKARDKTRMATIRLLMTEVREREVDVGHE
                     LSDEELQEVVSTQAKRRRESAEAYDEGARPELAEQERAELAILKTYLPEQLSAEELDA
                     IIDEAVSATGASGPGDLGKVMGRVMGRVKGRADGSEVRRRVAERLGG"
     gene            complement(11745..14192)
                     /locus_tag="VF097_03425"
     CDS             complement(11745..14192)
                     /locus_tag="VF097_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX6261880.1"
                     /translation="MATTHRATTRRVRAVDLERVRGLPDQALRVVLLIFVVAIAAGLF
                     ALALLPSVGAAGRGVQRFNDELNTIGTDIEIRYPRFPERSTIFAADGTVLATLFLDEN
                     RKIVKLRNVDQDLVDAVLAIEDARFYEHPGLDYRGIIRALVTNVGAGDIEQGASTLTQ
                     QLARNVYADVGTERTLARKIAEARVAMRMEDEYSKDQILELYLNEIYMGRSVYGVGTA
                     AEYYFGKHVRNLELHEAAMLAGLIQAPERYSPVVDKEAALSRRNVVLDRMAELGMITP
                     QEAAEARGRRLGLQPKNVVEKRKFPFFVDFVRREILDLENKAFDELGTTARQRQRALF
                     QGGLQIHTTLEPKLQRAGERVSRQHLPLPEDPQNAIASVEAATGRILALVSSQEFERS
                     QVNLATGQGGSGRQAGSAFKPFTLVTAFEQGIPPTKVYNGANGQTIPACGDYRLVNAD
                     GGGGFMDIWAATRGSVNAVFVQLAVDAGLEANVEVAHRMGIESPLGAFCSLPLGTNEV
                     TPLEMASAYATLANGGKRCERHAIDTIEDRSGDVIVKQKRRCKQVVDPDIAALTVNLL
                     EGVIEAGTGTAANLGTWPAFGKTGSTNNLADAWFAGCTKQICSASWVGHTKGLIPMSN
                     VHGERVFGGTFPARIWHDFMMIAMQGKPAIDFPGVPQISWPKAQVPDVVGLTEEEAMR
                     VLGEAGFTAQSTEVDSSEPAGTVVGQSPQGGARVPVSTTVTIQVSTGKAPRARVPDVV
                     GMSEGQAIRALERAGFAAAVSYETTNRKNLDGIVGAQAPGAGTRAEEGATVSITVHRY
                     EKPKPKPSPTPEPSPTA"
     gene            14332..14613
                     /locus_tag="VF097_03430"
     CDS             14332..14613
                     /locus_tag="VF097_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX6261881.1"
                     /translation="MFWEERAACGGFDPELFFGNTAADERRAKAICGTCAVAVECLSV
                     ALTSGMDFGIWGGLNERERRQVRRRNPGTRDWRALIEERGIRREPVRAG"
     gene            14653..15996
                     /locus_tag="VF097_03435"
     CDS             14653..15996
                     /locus_tag="VF097_03435"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEX6261882.1"
                     /translation="MRRLGLVLMAAGVLLGGPGAAAQEQAAYVDVIELEGVIDPTTAN
                     YLADEIDRSVETGSGLVVIQIDTPGGLDVSMREMVATILASEVPVAVWVGPPGARAAS
                     AGVFLVYASHIAAMAPATNIGAAHPVNLGGGETPDAAEEKAVNDAAKFLRSLAERRGR
                     PTDFAEAAVRESESLSAQEAADREVVEVLSPSLTDLLAQLDGREVETDRGTETLVTVA
                     TEDQPLEIRFHQMGLLRQILHAVSNPTVAFLLLTLGFWAIIFEISQPGFGIAGVGGAI
                     ALVLAFYALAVLPVNLAGLILVLLGLVLFTIDVFVQGLGVFTIGGALAFGIGGFLLFA
                     GASPEFRISPWVIVGLLIGSVLFFGFAMTAAWRARRRASMTGQEGLVGLTGEARADLD
                     PEGHVWVKGALWKARALGEPIPEGSRIRVRRVDGLLLLVQREEQKETAGVREGEA"
     gene            15993..16844
                     /locus_tag="VF097_03440"
     CDS             15993..16844
                     /locus_tag="VF097_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003995999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX6261883.1"
                     /translation="MSTGMIVLLVILGVVVLLIMAAIRIVQEYERGVIFRLGRVLPAP
                     KGPGLFFIIPIIDRVVKVNLQTVTMNVPPQDVITRDNVTVRVDAVCYFHVVDPIRATV
                     EIQNYLVGTSQISQTTLRSVLGKAQLDDLLAERERLNDELQTVIDELTEPWGVKVTLV
                     EIKDVDLPDNMQRAMARQAEAERIRRAKVINAEGEFQAAAKLSEAASVIARHPIAYQL
                     RLLQTMAEVSAEKNSTLVLPIPIELLRFFERARPVIEEGGGDPEEIGRALREAGVAGE
                     AEPEEEA"
     gene            complement(16841..17521)
                     /locus_tag="VF097_03445"
     CDS             complement(16841..17521)
                     /locus_tag="VF097_03445"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6261884.1"
                     /translation="MDLSEETFERVFQTARALGEGTRFRIFRRLCGAAPEPVSVRDLT
                     ESFDLHPNAIRQHLARLEQAGLVTSSPRREGGAGRPRRVYLATPEGLRVGATRGSPGA
                     LLAVLEEALQALPADRETLVEFGRAWGRAWARHRRSESGPTRGRRARAELLATELAIW
                     GWEPTTDRHNGTMSVATGRCLFRGASPGSNGRCCALEEGFLSGLAEGLLEGRATVALD
                     GCRLALSV"
     gene            17758..18030
                     /locus_tag="VF097_03450"
     CDS             17758..18030
                     /locus_tag="VF097_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007030715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX6261885.1"
                     /translation="MNEWVMGWQYRAACRGEDSSVFFAPNYFERKDEKEAREAKAKAL
                     CVRCPVRLECLDYALRTREPHGIWGGLNEVERRALIREREGTRQAG"
     gene            18032..18484
                     /locus_tag="VF097_03455"
     CDS             18032..18484
                     /locus_tag="VF097_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX6261886.1"
                     /translation="MIEERLQELGIELPPAAAPVASYVPVKRGPGIAFVSGQVPMEDG
                     APIVTGKMGAEVSLERGVELARRCALQALAALRDELGSLDAVKQVLRVGVFVASAPDF
                     TDQPKVGNGASDLLVEVFGEAGKHARAAVGCPSLPLDVPVEVELTVEI"
     gene            complement(18488..20347)
                     /gene="selB"
                     /locus_tag="VF097_03460"
     CDS             complement(18488..20347)
                     /gene="selB"
                     /locus_tag="VF097_03460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEX6261887.1"
                     /translation="MTDPLHVVATAGHVDHGKSSLIVRLTGIDPDRWEEEKRRGLTID
                     LGFAWCALPSGREIGFVDVPGHERFIRNMLAGMGPVKLVLFVVAADEGWKPQSEEHLA
                     IADVLGAQAGVVALTKRDLVDDARLAERTDDVRARLAGTVLEASPVVPCSATTGEGLD
                     DLRRALDEMVAAAPRPEDRDRPRLFVDRSFTIKGSGTVVTGTLTGGTLSVGDEVALLP
                     SGARTRVRSLQTHRRSIEVARPVSRVAANLAGTATGEVERGDALVRPRQWRPTGAIEV
                     HLRTVRSLGDPLTNRGAFKLHLGSAERDASLRLYGTRELAAGQETPARIRLSGPVVAA
                     VGDRFVLREVGRRETVGGGVVLDTDPPRRADEARLARRERAPIEDLPALLVAERGGVR
                     VSEIEPLTGASPAEISGATRLGSWWVHDETLGRLRDVVSEALEAHHRDRPLLPGMDRA
                     VLRDRLAMADPSLASHLEADLAASLLERLESEGLIAREGPLLRLAGHRVALGAREEEA
                     GRVVAAVEAGEPTPPDVPTLLEHGFDRELIDAVCRVGRLARVSKEVVVTPAFLERAER
                     VAREQAGSGEGLTVSGFRQALGTTRKYALPLLGSFDERGITRRDGDVRRLVDG"
     gene            complement(20344..>20674)
                     /locus_tag="VF097_03465"
     CDS             complement(20344..>20674)
                     /locus_tag="VF097_03465"
                     /inference="COORDINATES: protein motif:HMM:NF015780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEX6261888.1"
                     /translation="LPLWRQLAATPASVRRRARALAKGLDGAEAVEAETVVGGGSLPG
                     YALPSWAVRLAVADPERTAARLRAGNPPVFCRVDEDALLFDLRTVEPADDDRLARAIG
                     YALAQEA"
BASE COUNT         3035 a         7176 c         7593 g         2870 t
ORIGIN      
        1 gtcgagccgc tggatgagcg tcatcgcaag catgatcgcc agggcgaacg acgcgccgaa
       61 gacgagcgct ccgacgaacg cgagccagag gaaggggagc agggccatcg cgatcaggac
      121 ccccccggcg gtggcgacgg cgacgcggaa cgcgagcgcc tcgcccctgc gcacgaagag
      181 ccgggcgctc cccagtccgg ccgccatccc gagcccccac agcgacacga ggtagccgaa
      241 ctcgacgtcg ccccctccca gggtctcccg gacgtacgcg atcccgacgg cgaacagcac
      301 gccgcccccg agcatcgcca cgcccatgcc cgcggccagg ccgcgaaccg tgggcgatcg
      361 ccgcgcgaag cggaaggcct cgcccagccc ctcgaccacg cggaggtccg tccgaatcct
      421 ccgcttgggg atggggatcc tcgcgatcat gacggccgcc acgacgaagg tcagggcgtc
      481 gaagaggaag ggcacggcga gcggctgttc cggaaggggc gtgagcagcc ggaggcccga
      541 gaagagggcg gccgcgaacg ggatcgcgcc gtaggaggtg gccaggacga atccgttcgc
      601 ctgggggagc gagtcggggg gcacgagatc ggggacggcc gagtcccggg ccgggaggaa
      661 cagcatcgcc aggatctcgg aggcgaacgc gatcgcgtag aggagagcga gccccgcgaa
      721 cggcgccagc gagatgagga cggccatgcc gaggttggtc aggaccatca ggcggcgacg
      781 gtcgaaccgg tcggcgagga cccctcccag cggcgcggcg aggaggggcg gcagcagacg
      841 gatgacgagg acgccgccca ccgcggcggg ggatcccgtg agctcgtagg ccgcggcgat
      901 gaaggcgagg gtgccgaccc agtcgcccag cgaggagacg gtctgcccgg ccaggagcgt
      961 ccggaagggc ccccggccga ggatcccggc ccgttcttcc cgggcggggc gctcctcggg
     1021 cgacaccgtc aggccgtcgg gggcggcggc gacgagcgga gccgacgcac cgcctcgacg
     1081 aggatgcgat gggcgagctc gggggtcgtc cggagcagac ggtggaagtc gtcggcgacg
     1141 aggcggagcg cccgcagcct ggtggtggcc cgaacggtcg ccgagcgcgg ctcgccgtcg
     1201 atcactgcga gctcgccgaa gtggtcccct ggtcgcagcg acgcgatgtg ccggcctccg
     1261 gagacgacct cggcctcgcc gtcgaggatc aggtagaagt cgatggccag catgccctcg
     1321 tccaccatcg actggcccgc gtcgcacgtg acctcgaccg tggcgtccag caccgaccgg
     1381 atctggtccc tggtcaggcc ctcgaagagg gggacctcgg ccagcgcccg ctcggcgctt
     1441 cgcgtcatgc gctcaccccg tctcgcatcg taggagactc cggccctccc gcatagcccg
     1501 cggggtggcc ctcgctcgct cggctcacga gaatcacctc atggtcgggg tcgtgaccga
     1561 cagcgccgcg ggactgccac ccgagatggc ggcccggatg gggatcgagg tcgtgccgct
     1621 gtacctcagc gtcggcgggc ggagcctccg cgacggcgag ctccccgatc tatacgagcg
     1681 gctggagcgg gagcccggga gcgtgacgac gtcgatgccc acgcccggcg acttcgagga
     1741 ggcgttcgcg cggcgacccg aggaggagct cacgtgcgtc accctcgcgg cgtcggtgtc
     1801 gggtctgcac ggggcggcgc ggatggcggc ggaagccatc cggaagcggg tcgaggtgat
     1861 cgactcgggc ggcgcctcga tgggagaggg gtgggtggcg ttggcggcgg cgcgggcggc
     1921 ggccgcggga gggtcgctgg aggaggtggc cgccgccgcc cgcgccgccg ccgagcgcac
     1981 ccggctggtg gccaccatcg acacgatgga gcacctccgc cggtcgggcc gggtggggtg
     2041 gatcgcggcc ctggcggcga ccggcctcca gctgaagccg gtcttcgaga tggcccgcgg
     2101 agagatcagg ccggtggcca agccgaggac gcggcgacgc gcgctcgacc gcgtggccgc
     2161 ggaggcgctg cgggacctgg agggcggtcg agcgcgcgtc gccgcgatcc acgcccgggc
     2221 cgaggaggag gcccgagacc tcctggagcg ggtccgcccg aacacagaag tcctcgagga
     2281 gctcgtggtg gagttcacgg cggtgatggg cgcgcacacg ggccccggcg tggtcggcct
     2341 cgcctaccac ctcgaggcgt cgccgtgacg gaggagacct ggttcgggcc ggcctgggtg
     2401 ttcctcgcct acctcgtggg gagcatcccc tccacctacc tcgtggcccg ggtccggggg
     2461 gccatcgagg tgctgcgact cgcctccagg aaagccagcg agctcgacgc ccatatactc
     2521 ctgactgcgc acgccggccc gcggtggtcg ggggtggccg gcgtcgccga cgtggtgaag
     2581 gcgctgctct tcgccctcgc cagtggctgg gtcggcttcg agccgccctg gcaggccgcg
     2641 gccggcgtcg cgctcgtcgc ggggtactgc tggccgccct acgcctcgcg cttcgccggc
     2701 cgcggcctcg cggtcgccgc cggagtctcc ctggccctcc tcccgctccc cacgctcgtc
     2761 ttcggcctcc tgaccctggg cggcgttgcc ctgggggcga ccggcctctg gtcgacgatc
     2821 gggctgctct cgatcccgct gacggcctgg ctcctcgacg ctcccggggc gctggtcgcc
     2881 atggcagccg ccatcctcgc gctgatcctc ctccgcagga tcgaggggct gggccgggcc
     2941 gagggcgtcc cgctggcccg cgccgccctg cggagagccg tgttcgaccg cacctcctag
     3001 gccccgtccc actgctaggc tcgcggaggc atgggcatcg tggtgcagaa gtacggcggg
     3061 acgtcggtgg ccgacctgga ccggatccgc tccgtcgccg gtcgcgtgat cgaagcgaag
     3121 gaggccggcg acgaggtctg cgtcgtcgtc tcggcgatgg gggatacgac cgacgagctc
     3181 ctgggtatgg cccaccggat cacccccgtc ccccacgggc gggagctgga cctcctcctc
     3241 accgccggag agcggatcgc catgagcctc ttggccatca cgatcaacgc cgagggctac
     3301 ccggccgcct cgtacacggg ctcgcaggcc gggatcatca ccgacaaccg gcacggcaag
     3361 gccaagatca tcgacatccg cccggggagg gtccgcgagg cgctcgatcg cggagacgtg
     3421 gtgatcgtgg ccggcttcca gggcgtctcc acggctcacg acgtgaccac gctcgggcga
     3481 gggggctccg acaccactgc ggtggcgctg gcggccgcgc tgggggccga ggtgtgcgag
     3541 atcaacaccg acgtgggcgg agtctccacc gccgaccccc ggatcgtccc cgaggcccgc
     3601 aagctccacg cggtctccta cgaggagatg ctggagatgg cggccacggg ggcgaaggtg
     3661 ctcgagctcc gggccgtgga gtacgctcgg aacagcggga tcaagctcca cgtccggtcc
     3721 tccttcgaca gcgagggagg cacctggatc gcggaggagg acgagcggat ggagcaggcg
     3781 atcatctcgg gggtgaccca cgacacctcg caggccaagg tcacgctggt ggacgtgccc
     3841 gatcggccgg gcatcgcggc cacgatcttc aaggcggtgg ccgacgaggg cctcaacgtc
     3901 gacatgatcg tccagaacgt ctcgcgcgag ggcatcacgg acctctcgtt cacgatcccg
     3961 aaggacgacc tcccccacgc tgggaaggcg atcgggcgcg tggccgagga gatcggcgtt
     4021 gcctacgaca ccgacgccga catcgccaag atctcgctga tcggggccgg gatgaagacc
     4081 catcccggcg tcgcggcgcg gatgttcgac gcgctggccc gagccgggat caacttggag
     4141 atgatctcga cctcggcgat ccggatctcc tgcgtgatcc gggcctcaga cgtggagcag
     4201 gcggtgcgcg tgctccacga cgagttccgg ctctccgagg aggacgtgat ccgcgagcac
     4261 gagggcgcct aggcgggagc ggggaccggc tcgcgccggc gctcccggcg gctcgccagt
     4321 cctgcgcccg cgatgatgag cgcgatcccc gcgaagacga ccggctccac cgtctcgtcc
     4381 cggaacacca cgcccaggat cgtggccacc acggggatga agtagatggc cagcgcgcct
     4441 ctggtggcac cggccctccc cacgaggacc gccatagcca cgagcgccac cccagtggac
     4501 aggaacccga ggatcaggac ggcgccgagc gacgagaggc ggaacgacga gccggggatc
     4561 gagatcagac catacgggag catcgccacc accgaggcga gctgagcccg ccacacgacc
     4621 ggaagggccc cgtacttctg ctggaggggg acggccaggt tggcggccag cccgtacagg
     4681 atcgtggcca ggaggaccag cccgatcccg aggaggcgcg accctccctc gcccgccgcc
     4741 ggcagcgaga cgagcacgac cccggcgaac cccacggcca ggccgatcag ctgccgggcc
     4801 cccggcatcg tccgcagaag gagcgtggcg acgaccgcgg tgaacagcgg catcgcgccg
     4861 ttgatcatcc ccgcggtcga ggagtcgatc cactgctgcg ccaccgggaa gagcgtgaac
     4921 gggatggcca cccacaccag cccgaggagc gcgacccggg gccagtcctc gcgctcgatg
     4981 ccgcgccggg ctcgcgacat cagccccagg aacgaggctc ccagcgccac gcggaggaac
     5041 gcgatcagcc ccggctcgaa cccctcgagc ccgatcgcga tccagaggaa cgacgatccc
     5101 cacatcagcg cgatcgaggc gaggaggccc cactcgacgg ggccgaacgc ctccgaacgg
     5161 gtcccgtggg aagtctggat cagcttggcc atcgcagagc aagaaccctc ctcggtccgg
     5221 gtatgttccc ggcatgcgaa ccgtggacta cgtgatccgg tacgtggagg acctggaccg
     5281 atcggtggcc ttctatcggg acgtgctggg cctcgagcac cgcttcacgg acgaagcgta
     5341 cgcggagttc tcggcggggg gtacgaagct gggcttcttc cctcggtcga gcttgccgga
     5401 cctgctgggg cgggaggcgc cggggggcgg gccgcccgac ggcgagatcg tcctcctcgt
     5461 ggaggacgtg gatgcggagg cgcagcggct ccgggacgcc ggggtggaga tcctcaccgg
     5521 tcccgtggac cggccgtggg gtcaccgcac gatccatctg ctcgatcccg atgggctcgt
     5581 cgtcgaggtg gcccaggaga tcgagcgcgc ctagcaggca gcggtcaagc ccggaagggg
     5641 catgcgcagg ggatctcccc gcacttcggg cagccgtccg cgtagcggct catcgcctcc
     5701 tccagatcca cgtttgccac gttggccagc gacgtgagcc aggcgagagc gtcggccacc
     5761 tcgtcccgga tcgcctccgg atcgtcccgg cgaagggccc gcgccacctc cccgatctcc
     5821 tccacgagcc agcggaaggt gccgtccgcc ccgcgacgga gatcccgctc cagataggtg
     5881 cgccgcatca ggtcctgcag ctccgagatc cgcatcccga tctcagacgt ccttgggcgg
     5941 cagcggggga ccgagcacca tctggccgta gcgccgcatg atctccacga gctcctctcg
     6001 atcgcgcaga ggctcgtccg caggcgggag ctggagcgag cgagcggggc cgcccgcgtc
     6061 gcggaagaac ccctcgaccc cggccgggcc gaagaggagc agcagccgtg cctcctccga
     6121 atccgcaagg aacgcgtggc cgaccccctt cggcgtgtac gcgaacgtgc cggggacccc
     6181 ctcgatcacc tcatcgccgt gctgcagacg gacctccccg gacagcacga tgaacaattc
     6241 gtcttcgtgc tcgtgggcgt gcaggggcgg gccgaagccc ttggggatcc ggagctcgta
     6301 ggcggtgtat cgtccctcgg tctggtcggc cgtcgccttc aggatcccga gcccgccgtt
     6361 gaaccagagg gcctcaccct cgtcgggccc cagcgcgaat cccttggtgg tctgcggcag
     6421 cgacatcgtc cctcccttcc ggtgcggtcg gggggaagga tacgctcgat cagcgccaga
     6481 gccctacgat cgacgcgacg acgacgagct tcaccagcca gtggccggcg tggatcgccg
     6541 cgaccttggg acgcgcttcg tcccagatca cggcgcccac gagcaggacc accgggaacg
     6601 ccatccacag cgcgagcgcg agcccggtcg cacccgccag atccggcacc tccaggagcc
     6661 cggcgagccc tgcgaccacc gccgccacgg tcaggctccg caccagctcg acgaggatct
     6721 tccacgcagg gggtcgagct tccgccgcct cggggctcaa tccccgagcc tccgcgtact
     6781 gacgtccgag cacgatgtag tacgccgtgc tgatcacgaa agcagacgcc acggccgtag
     6841 ccagggcggc gtagttgtgg tcgatcatcc gtaccctcct cggggccgca tgatcccggg
     6901 actcacgctc ctaggatcat cgacccacgg gtccggctga tcgctgtgcc tcctgaggcg
     6961 gagccccttt gccccaacgg gccgatggcg tgagcatcgg gtctggggtt cggcgtggga
     7021 tcgccgtcga aagagagacg gctcctgggc cgagaagtca tcggcaggag acatggatgc
     7081 cgcgagacat cgctgagtcg ggacggcggg atttgaaccc gcgacctcag cgtcccgaac
     7141 gctgcgcgct accaagctgc gccacgtccc gacggtccat ccagtgtagc gcgagccgcc
     7201 cggcccacca gggccgctac ttgttctgct gctcgagcgc gccgaccatc ccctcgggcg
     7261 actcgccctc catggggacc tcctgctcgg acaggtcgcg ctcggggatc ccctcctcgc
     7321 gccaccgccg cttgaggtag ggcttgagcg cgagggccgc gtcggcgttc gcctggatcc
     7381 ggtcctcggc ctcatggaga tccgcgtccg ccacctcttg gatgaaggtg gccgtccgcg
     7441 cgaagtacag ggggatcatc gaatcgatca gcgtgccggg gtccacccgc cgcccgttgt
     7501 aggcgatcag gaagtcgtag acgatcttcg tccaggtgtc gaggtccagc accagccccc
     7561 gcgtggtctc cgcggcatcg cctaccgccg tctggacgcc gtgcagcgac tcttccgaga
     7621 ggacctgggt ccacagcgcc tggtgcttca cgtatccctc tacgaacttc caggtgagcc
     7681 ggggcaccga gaccttgatc ggctcggccg agtaggcggc ggggaagccg aagatcgggg
     7741 gcgaagtcgc ggtgtcggtc tcccaccagt cgttccggta gcggccggcg agcgcgaaga
     7801 ggctgcccac gacctggcgg aacatcgggc ccaggtcctt gccggggtcc ttcgcgtcgt
     7861 gcagcttggc gccgaggata ccctgggcca tcttcgcgcc ctccacgagc gccacggtcg
     7921 tcatccagat gtcgatcccg aacctcgcca cgtccgactc ccacacgttc ttcgccgcgt
     7981 aaaccttggc cagctcgccc gagaacccga actccccacc gatcggctga cggatccgca
     8041 cgccgtagag ggccgtcgtc accgggaagg cgatgcagtt cgtgatcgtg ccgtcgaact
     8101 tgtggcgtgc gtagatcggg gcgacgtact cgtacccgta gtggacgatc ggggagagga
     8161 tccggtcgag ccagtagggc gtgatgctcc gcaggtcgga gtccacgacc ccgcatgcgg
     8221 ccacccccag tctcgcggcc acctcgaaga tcgagcggaa ggcgctgccc ttccccgaca
     8281 ggccgctgta gggaaacgcg atccgcttcg ggaccggaga gtctcccggg acgagcagga
     8341 gcttccagtc ctccccgatc tcggcggact gtgccacggc cacggtccgg tcggcggatc
     8401 cgccgtcgga gacgcagatc acggatcgcc gctcggggaa gtgctttcga aggccggcct
     8461 cgacggcgcg gatgacgccc ccgatcgtct cctcgttgtt gaagcagggg atcccgacca
     8521 ggatgtcggc gctcccgatc tcgtccagct ggaacagcac gtcctgcggg agcaggtcgt
     8581 cgttggccgt cgctgccacc ccttcctggg agtcgacctc aggtgctagc ataccgggtg
     8641 ccctgatcgg gcatgcccgg aacggaggac ccatggagcc ccgcgactgc acctggtgcg
     8701 gaagcgcccg atccgtcgag cacgacgtct gccaggtgtg cctgatgcgg ttccccgagc
     8761 tcaagaacgc ggacgaagtc gtcgtgatcc tcgacgagcg ggagctgcag atccactcgg
     8821 tccccgccga ggaagaggaa gcggcgcgca ccgccgtctc cgagtagcat cggccggatg
     8881 cggatcgccc aggtctcccc gccgtgggtc gccgtcccgc ccaagggcta cggcggcatc
     8941 gagtgggtgg tctccctcct ggcggacggt ctcgcggagc ggggccacga cgtcaccctc
     9001 ttcgccaccg gcgactcgca gaccaaggcc cggctggagt acgtgttcga ggcggcgccg
     9061 gggtccaccg cgatcaactc gatctggcac gacaccgtcc atcagatgct cgtccacagg
     9121 gatttcgggc ggttcgacgt gctgcaccac cacacgccgt tctcggcgct cgtggccgcg
     9181 atgtgccacg acgtaccggt ggtccacacg ctccacggcg acttcacgcc gcagttccgc
     9241 cttctctacg agcaggtggc cgaccgcctc acgttcgtgg cgatcagcca gtcccagcga
     9301 gccgccatgc ccgagcttcg ctacggaggc gtcgtctaca acgggatcga cctcgatctc
     9361 tacccgttcc ggtcggagaa ggaggggttc ctctgcttca tcggccgtgc caaccccgac
     9421 aagggggctc accgggcggt gctggccgct cgcgcggccg ggatgccgct cgtgatggcg
     9481 gtgaagatgg cacaccccgt ggaacgggag cactggacgc gcgaggtgga gccgctgctg
     9541 gggcccgacg tcaccgtgct cggcgagatc tcggtggagg agaaggccga cctcatgtcc
     9601 cgcgctcggg ccgtcctgtt cccgatcgac tggcccgagc ccttcggcct cgtgatgacc
     9661 gaggccatgg cctgcgggac gccggtgatc gccacgccgc gcggcgcggt gcccgaggtg
     9721 atcaccgacg gccggacggg gttcctggtg agcgtggagg ggtatccgga ggaggccgcc
     9781 cacgcgatcg ggcggctggg agagatcgac ccggaggcgt gccgcagcca cgtggccgat
     9841 cgcttctcca aggagtcgat ggtcgaaggc tacgagcggg tgttcgagcg ggtcgtggag
     9901 ggaggcagat ccggtgactg acgtgaaggc gtcgatcgag aaggccgtcg cctacctgac
     9961 cgagcatccg gacgacgcgc gctacacgga ctcggcggcg acggcgaggc tgggtgacgc
    10021 gctgagggtg gaggtggagg ggccggccgg cgagcggctg acgaccgaca tgccgggggg
    10081 cgtggggggc cgtggagagc tacccacccc cggctggctg tttcgggccg ccgtcgcctc
    10141 gtgcgtggcg tcgacggtgg ggatggaggc cgcccgcgag ggcgtcgacg tgggggatgt
    10201 ggaggtcctg gtggacagcg agtcggacga tcgggggatc ctcgggatcg acgacggcgt
    10261 gccggcggga ccgctctcga tgcggatccg catctcgctc cgtggcggag aggcctcgga
    10321 tcgagcgatc gaggtggccg agcgcggcgc cgctcggtgt ccggtgtgcg acgcgacgaa
    10381 gcgggcggtg gaggtctcgg tcgagatcgg ctgagtcatc cggccgagcg cagctcgagc
    10441 atcgtcgcct cggggcggca gaacagcctg aagggcgcgt acttcgaggt ccccagacca
    10501 gggctgacgt gcatgaaggt ccggccgatc cgatggaggc cgctggccat ccgccggggg
    10561 atcgaggagt tggtcacgat ggctcggccc cacggcagcc ggacctgccc cccgtgggtg
    10621 tggcccgtga cgatcaggtc ccagccgagg gccgcgagct cgggcgccgg atcgggcgag
    10681 tgcacgaccg ccaggccgaa gcggcccgtc cccctccgag gcgaaaggct cacgttcccc
    10741 cggtggatgt gggggtcgtc gaggcccagg atctccatgg gcacgccgtc caggctcccg
    10801 ccccgacgga ggttgcggag gtggacccag ccgtcggcct cgagctgggc tacgaggtcg
    10861 ggtgcgcggc cgcgccgccc ccgccgccgc ctgcgcttct cgtgggggac gaagtagcgg
    10921 agcgggttca gcgggcgcgg cacgtacacg tcgttcgagc ccaggacgaa catcgagccc
    10981 agccgccccc gcagggggcg gagggactcc acgacgtact ccacggcctt cggctccccc
    11041 agcaggtcgc cggtgatgat cgtgacgtcg gcccgaggca ggctctccag gaaccgccgc
    11101 ttgtgcgcct cgccgtcgac catgtgcagg tccgagaggt gcaggatcgt gagcggcccg
    11161 ccccccggga ggatgtcgag gcggtagcgg gccaggcgat accatcgacg ctcgatcagg
    11221 atggcgtagg cggccaccag gagaccggcg gcgaggaggc cgagcaggat ccaccagagc
    11281 acggaggcga ttgtatggga gccctcaagg atcggctgtc gatggagctg cgcgaggcga
    11341 tcaaggcccg cgacaagacg cggatggcca cgatccgcct gctcatgacc gaggtccgcg
    11401 agcgggaggt cgacgtgggg cacgagctgt cggacgaaga gctccaggag gtcgtctcca
    11461 cgcaggccaa gcgcaggagg gagtcggccg aggcctacga cgagggcgcc cgccccgagc
    11521 tggcggagca ggagcgggcc gagctggcca tcctgaagac ctacctcccc gagcagttga
    11581 gcgccgagga gctcgacgcg atcatcgacg aggccgtgtc ggcgacaggc gcctcgggcc
    11641 cgggcgatct gggcaaggtc atgggcaggg tgatgggccg ggtgaagggt cgggccgacg
    11701 ggagcgaggt ccgtcggcgc gtggcggagc gcctcggggg ctgatcaggc cgtcggggag
    11761 ggctcggggg tcggcgaggg cttgggcttc ggcttctcgt accggtggac ggtgatcgag
    11821 accgtggccc cctcctcggc tctggtgccg gcgccggggg cctgcgcgcc cacgatcccg
    11881 tcgaggttct tccggttcgt cgtctcgtag gagacggcgg cggcgaaccc ggcgcgctcc
    11941 agggcccgga tggcctgtcc ctcgctcatc ccgaccacgt cgggcaccct ggccctgggg
    12001 gccttcccgg tggagacctg gatcgtcacg gtggtgctca ccgggaccct ggccccgccc
    12061 tgcggggact gcccgaccac cgtgcccgca ggctcgctcg agtcgacctc ggtggactgg
    12121 gcggtgaagc cggcctcgcc cagcacccgc atcgcctcct cctcggtcaa cccgacgacg
    12181 tcggggacct gggccttggg ccaggagatc tgcgggaccc ccgggaagtc gatcgccggc
    12241 ttcccctgca tcgcgatcat catgaagtcg tgccagatcc gggcggggaa ggtgccgccg
    12301 aagacccgct ctccgtggac gttcgacatg gggatcaggc ccttcgtgtg gcccacccag
    12361 gaggcggagc agatctgctt cgtgcagccc gcgaaccagg cgtcggcgag gttgttcgtc
    12421 gagcccgtct tgccgaacgc gggccaggtc cccaggttgg ccgccgtccc ggtcccggcc
    12481 tcgatcacgc cctccagcag gttgacggtg agcgccgcga tgtcggggtc gacgacctgc
    12541 ttgcagcggc gcttctgctt cacgatcacg tcccctgagc gatcctcgat cgtgtcgatc
    12601 gcgtggcgct cgcagcgctt gccgccgttg gccagcgtgg cgtaagcgga ggccatctcg
    12661 agcggtgtca cctcgttcgt gccgaggggg agcgagcaga aggcgcccaa cgggctctcg
    12721 atgcccatac ggtgggcgac ctcgacgttg gcctccagcc cggcgtccac ggccagctgc
    12781 acgaacaccg cgttcaccga cccgcgcgtg gcggcccaga tgtccatgaa ccccccgccc
    12841 ccgtccgcgt tcacaagccg gtagtcgccg catgcgggga tcgtctggcc gttggctccg
    12901 ttgtagacct tcgtgggagg gatcccctgc tcgaaggccg tgaccagcgt gaacggcttg
    12961 aaggccgacc ccgcctgccg ccccgagcct ccctggcccg tggccaggtt cacctgcgag
    13021 cgctcgaact cctggctcga gacgagggcc aggatccggc cggtggccgc ctcgaccgag
    13081 gcgatcgcgt tctgggggtc ctcggggagg gggaggtgct gtcgggacac ccgctccccg
    13141 gcccgctgga gcttcggctc gagcgtggtg tggatctgca gcccgccctg gaagagcgcg
    13201 cgctggcgct ggcgggccgt ggtccccagc tcgtcgaagg ccttgttctc caggtcgagg
    13261 atctcccggc ggacgaaatc cacgaagaac gggaacttcc gcttctccac gacgttcttg
    13321 ggctggaggc ccagacgcct gcccctcgcc tcggcggcct cctggggggt gatcatcccg
    13381 agctcggcca tgcggtcgag caccacgttg cgccggctga gcgcggcctc cttgtccacg
    13441 acgggtgagt accgctcggg cgcctggatc agccctgcca gcatcgcggc ctcgtgcagc
    13501 tcgaggttcc gcacgtgctt cccgaagtag tactccgcgg ccgtgcccac cccgtagacg
    13561 ctccgcccca tgtagatctc gttgaggtag agctcgagga tctggtcctt cgagtactcg
    13621 tcctccatgc gcatggccac tcgggcctcg gcgatcttcc gggccagcgt gcgttcggtc
    13681 ccgacgtccg cgtacacgtt ccgcgcgagc tgttgggtga gggtcgaggc gccctgctcg
    13741 atgtcgccgg cccccacgtt cgtgacgagc gcccggatga tgccgcggta gtcgaggccg
    13801 gggtgctcgt agaagcgggc gtcctcgatc gccagcaccg cgtcgaccag gtcctgatcg
    13861 acgttccgca gcttcacgat cttccggttc tcgtcgagga agagcgtggc caggaccgtg
    13921 ccgtcggcgg cgaagatcgt ggagcgctcc gggaaccgcg ggtaccggat ctcgatgtcg
    13981 gtcccgatcg tgttgagctc gtcgttgaac cgctgcaccc cgcggcccgc cgcgcccacg
    14041 ctcgggagca gcgccagcgc gaagagcccc gccgcgatcg ccacgacgaa gatgagcagg
    14101 acgacccgga gggcctggtc ggggagtccc cggacgcgct cgaggtccac ggcccgcacg
    14161 cggcgtgtgg tggctcggtg ggtggtggcc atgggatccg gaacaggata gcgagcgatg
    14221 ggggccgcgg ccatgggact cccggtggga aacctgtgga aaccgtgtgg aggaaaattg
    14281 ggccgatcgg actatccaca gcgcactgtc gcgcgactac catgccgggc catgttctgg
    14341 gaggagcggg cggcctgcgg cggcttcgat cccgagctct tcttcggcaa cacggcggcc
    14401 gacgagcgcc gcgccaaggc gatctgcggg acctgcgcgg tggccgtcga gtgtctcagc
    14461 gtcgctctga cctccggcat ggacttcggc atctgggggg gcctcaacga gcgggagcgt
    14521 cgtcaggttc ggcggcgcaa cccggggacg agggactggc gggcgctgat cgaggagcgt
    14581 gggatccgtc gtgagcccgt ccgcgccggc tgagcccggc gaacggagga gacgccccct
    14641 aggatagggg gcgtgaggag gctcgggctc gtcctgatgg cggccggcgt cctgctcggt
    14701 gggccggggg ccgccgcgca ggagcaagcc gcgtacgtcg acgtcatcga gctcgagggc
    14761 gtcatcgacc ctaccaccgc gaactacctc gccgacgaga tcgaccgatc ggtcgagacc
    14821 ggctcgggcc tcgtcgtgat ccagatcgac acccccggcg gcctcgacgt ctcgatgcga
    14881 gagatggtcg ccacgatcct cgcctcggag gtccccgtgg ccgtgtgggt gggtcccccc
    14941 ggggcccggg ccgcgtcggc gggggtcttc ctcgtctatg cctcgcacat cgcggcgatg
    15001 gctcccgcca cgaacatcgg ggcggcgcac cccgtgaacc tgggaggcgg cgagacgccc
    15061 gacgcggccg aggagaaagc ggtgaacgac gcggcgaagt tcctgcggag cctggccgag
    15121 cgtcgcgggc gccccaccga cttcgcggag gcggccgtcc gagagagcga gtcgctctcg
    15181 gcccaggagg ccgcggatcg cgaggtggtc gaggtgctct cgccgtcgct caccgacctg
    15241 ctggcccagc tcgacgggcg tgaggtcgag accgaccggg ggaccgagac cctcgtcacg
    15301 gttgccaccg aggaccagcc cctcgagatc cggttccatc agatggggct gctgcggcag
    15361 atcctccacg cggtctcgaa cccgacggtg gccttcctcc tcctgacgct ggggttctgg
    15421 gcgatcatct tcgagatcag ccagccgggg ttcgggatcg caggggtggg cggggcgatc
    15481 gcgctcgtcc tcgccttcta cgcgctggcc gtgctcccgg tgaacctggc cggcctgatc
    15541 ctggtgcttc tgggcctcgt gctgttcacg atcgacgtct tcgtgcaggg cctgggggtg
    15601 ttcacgatcg ggggcgcgct cgccttcggc atcggggggt tcctgctgtt cgcgggggcc
    15661 tcgcccgagt tccgcatctc gccgtgggtg atcgtgggcc tgctgatcgg gagcgtgctg
    15721 ttcttcggct tcgccatgac cgccgcctgg cgggcccggc ggagggcctc gatgaccggg
    15781 caggagggac tcgtgggcct gacgggcgag gccagggcag acctcgatcc cgaggggcac
    15841 gtctgggtca agggagcgct gtggaaggct cgggcgctgg gtgagccgat cccggagggg
    15901 agccgcatcc gggtgcgccg ggtggacggc ctcctgctgc tcgtgcagcg ggaggagcag
    15961 aaggagaccg ccggagtgag ggagggtgag gcatgagcac aggcatgatc gtgctcctcg
    16021 tgatcctggg ggtcgtcgtg ctgctgatca tggcggcgat ccgcatcgtg caggagtacg
    16081 agcgaggggt gatcttccgg ctgggccggg tcctgccggc gccgaagggg ccggggctct
    16141 tcttcatcat cccgatcatc gaccgggtgg tgaaggtgaa cctccagacg gtgacgatga
    16201 acgtcccgcc ccaggacgtc atcacccggg acaacgtcac cgtgcgcgtg gacgccgtct
    16261 gctacttcca tgtggtggac ccgatcaggg ccacggtgga gatccagaac tacctcgtgg
    16321 gcacgtcgca gatctcccag acgacgctcc ggtcggtgct ggggaaggcc cagctcgacg
    16381 acctgctcgc cgagcgcgag cgactgaacg atgagctcca gacggtgatc gacgagctca
    16441 ccgagccctg gggggtcaag gtcacgctcg tggagatcaa ggacgtggac ctccccgaca
    16501 acatgcagcg ggccatggcc cgccaggccg aggccgagcg gatccgccgg gcgaaggtca
    16561 tcaacgccga gggcgagttc caggcggcgg cgaagctgtc cgaggccgcc agcgtgatcg
    16621 cgcggcaccc gatcgcctac cagctccggc tgctccagac gatggccgag gtctccgccg
    16681 agaagaactc gacgctcgtc ctgccgatcc ccatcgagct cctgcgcttc ttcgagcggg
    16741 ccaggccggt gatcgaggag ggcggcgggg atcccgagga gatcgggagg gcgctccgcg
    16801 aggccggcgt ggccggggag gcggagcccg aggaggaggc ctagaccgag agggcgagcc
    16861 ggcagccgtc gagcgcgacg gtggcccggc cctcgagcag gccttcggcc agccccgaga
    16921 ggaacccctc ctccagcgcg cagcagcggc cgttgctccc gggggacgcc ccccggaaga
    16981 ggcatcgtcc cgtcgcgacg ctcatcgtcc cgttgtggcg gtcggtggtg ggctcccagc
    17041 cccagatggc gagctcggtg gcgagcagtt cagccctggc ccgtcggccg cgcgtggggc
    17101 cgctctcgct ccggcgatgc cgggcccagg cgcggcccca tgcgcggccg aactcgacca
    17161 gcgtctcgcg gtcggcgggc agggcctgta gggcttcctc gagcaccgcc agcagcgcgc
    17221 ccgggcttcc ccgagtggcg cccaccctca gcccttcggg cgtggcgagg tagacgcggc
    17281 ggggtcgacc cgccccgccc tcgcgtcggg ggctcgatgt cacgagcccg gcctgctcca
    17341 gccgggccag gtgctgcctg atcgcgttgg ggtggaggtc gaagctctcg gtgaggtcgc
    17401 ggaccgagac cggctccggg gcggcgccgc acagccgccg gaagatgcgg aacctggtcc
    17461 cctcccccag ggcccgagcg gtctggaaga cccgctcgaa cgtctcctcg gataggtcca
    17521 tgcgcctcga tgatagaccc gcgccccgat ccggatgggg ccgagggggc ctcccccggc
    17581 cgccccacgc cgccgccgaa tcggggcttg acaaacgctc cgggacccct gataccctgc
    17641 gcccaaggac atttccacag gtgaaattgg agaaacgggg tcgcctcggc cgggggagaa
    17701 acgcagcgaa ccgccgaggc gtttccatac gaagaactgg tgagggaccg aaccgtgatg
    17761 aacgagtggg tgatggggtg gcagtaccgc gccgcctgcc gaggcgagga ctccagcgtc
    17821 ttcttcgcgc cgaactactt cgagcggaag gacgagaagg aggcccgcga ggcgaaggcc
    17881 aaggcgctct gcgtgcgctg ccccgtccgg ctcgagtgcc tcgactacgc cctcaggacc
    17941 cgtgagcccc acggcatctg gggcggcctg aacgaggtcg agcgcagggc cctgatccgg
    18001 gagcgcgagg ggacccggca ggccggatga cgtgatcgag gaacgcctgc aggagctggg
    18061 gatcgagctg ccgccggccg ccgcgccggt ggcctcctac gtcccggtga agcgcggccc
    18121 cgggatcgcc ttcgtctcgg gccaggtccc gatggaggac ggcgctccca tcgtcacggg
    18181 gaagatgggg gccgaggtct ccctcgagcg gggcgtcgag ctcgccagac ggtgcgcgct
    18241 ccaggcgctc gcggcgctcc gcgacgagct cggctcgctc gacgccgtga agcaggtgct
    18301 gcgcgtcggg gtgttcgtgg cgtcggcccc ggacttcacc gaccagccca aggtggggaa
    18361 cggcgccagc gatctcctcg tggaggtctt cggggaggcg ggcaagcacg cgcgcgccgc
    18421 cgtaggatgc ccctccctcc cgctcgacgt tcccgtcgag gtcgagctga cggtcgagat
    18481 ctagtgctca accatccacg aggcggcgga cgtcgccgtc tcgtcgcgtg atccctcgct
    18541 cgtcgaagga tcccagcagg gggagggcgt acttcctcgt cgtccccagc gcctgacgga
    18601 agccggagac cgtcaggccc tcccctgatc ccgcctgctc ccgggccacc cgctcggctc
    18661 gctccaggaa ggcgggcgtc acgaccacct ccttggagac ccgagccaga cgccccaccc
    18721 ggcagaccgc gtcgatcagc tcgcggtcga agccgtgctc gagcagggtc gggacgtcgg
    18781 gcggcgtggg ctcgccggct tcgaccgccg caacgacccg ccccgcctcc tcctctcggg
    18841 cgccgagcgc gacccggtgg cccgcgagcc ggagcagcgg accctcccgg gcgatgaggc
    18901 cctcgctctc cagccgctcg agcagcgacg cggcgaggtc ggcctccagg tgggacgcga
    18961 gcgatgggtc ggccatggcg aggcggtccc ggagcacggc ccggtccatc ccggggagga
    19021 ggggccggtc ccgatggtgg gcttccaggg cctcggagac gacgtcgcgg agcctcccca
    19081 gcgtctcgtc gtgcacccac cacgatccga ggcgggtcgc gccgctgatc tccgcgggtg
    19141 atgcgcccgt gaggggctcg atctccgaga cgcggacccc gcctcgctcg gcgaccagga
    19201 gcgcgggcag gtcctcgatc ggggcgcgct cccgccgggc gaggcgcgcc tcgtcggccc
    19261 gtcgcggcgg gtcggtgtcg aggaccacgc ccccgccgac ggtctcgcgc cgtcccacct
    19321 cgcggagcac gaatcgatcg cccacggcgg ccacgaccgg gccggagagc cggatccggg
    19381 ccggcgtctc ctggccggcg gcgagctccc gcgtcccgta gagccggagc gaggcgtcgc
    19441 gctcggccga gccgaggtgc agcttgaagg ccccacggtt cgtgagcgga tcgcccagcg
    19501 agcggacggt gcggaggtga acctcgatcg cgcccgtggg gcgccactgc cgggggcgga
    19561 cgagcgcgtc gcctcgctcc acctcgccgg tggcggtgcc ggccaggttc gcggcgacgc
    19621 gcgagacggg ccgcgctacc tcgatcgacc ggcggtgggt ctgcagggat cgaacgcgtg
    19681 tccgggcgcc tgaggggaga agggccacct cgtcgccgac ggagagcgtc cccccggtga
    19741 gcgtcccggt gacgacggtc ccggatccct tgatcgtgaa cgagcggtcc acgaacagcc
    19801 gagggcgatc ccggtcctcg ggccggggcg cggcggcgac catctcgtcg agggcgcggc
    19861 ggaggtcgtc cagcccctcg ccggtggtgg cggagcacgg gacgacgggg gacgcttcca
    19921 gaacggtgcc ggcgagccgc gcccggacgt cgtccgtccg ctccgccagc cgtgcgtcgt
    19981 ccaccaggtc tcgtttcgtc agggccacga cccccgcctg cgcgcccagc acatcggcga
    20041 tcgctaggtg ctcctcggac tgcggcttcc acccctcgtc ggccgccacg acgaacagca
    20101 cgagcttcac ggggcccatc ccggcgagca tgttgcggat gaagcgctcg tggccgggga
    20161 cgtccacgaa gccgatctcc cggcccgagg ggagggcgca ccaggcgaag ccgaggtcga
    20221 tcgtgagccc cctgcgcttc tcctcctccc agcggtcggg gtcgatgccc gtgaggcgga
    20281 cgatcagcga ggacttgccg tggtcgacgt gtcccgccgt ggccacgaca tggagcgggt
    20341 cggtcatgcc tcttgggcca gggcgtagcc gatggcgcgg gccaggcggt cgtcgtcggc
    20401 gggctcgacg gtccggaggt cgaagaggag cgcgtcctcg tccacgcggc agaacaccgg
    20461 agggttgccg gcgcgcaggc gggccgcggt tcgttccgga tcggccaccg cgagccggac
    20521 ggcccaggag gggagggcgt agccggggag ggagccaccg cccaccacgg tctccgcctc
    20581 cacggcctcc gcgccgtcca ggcccttggc cagggcccgg gcgcgtctgc ggaccgaggc
    20641 gggggtcgcg gcgagctgcc gccacagcgg cagc
//
LOCUS       DASUTZ010000075         2099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1834572, whole genome shotgun sequence.
ACCESSION   DASUTZ010000075 DASUTZ010000000
VERSION     DASUTZ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1834572"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="VF097_03470"
     CDS             <1..905
                     /locus_tag="VF097_03470"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6261889.1"
                     /translation="HTDAGLRTVFVQVPGDGDDEDALLEGLVRLAPRAGPSTSIALGS
                     AEPFDADRLARRWAVARELGLRIHGHLASGSAAGTVARLASRGLLGDDVTLVHCSGLD
                     EKDLDAVADSGASVSLSPSSDMAEGSGSPPIQQLIDHGIRPGLGVDHERLAPGDAFAQ
                     MRATISLQHATVFDLKLAGKAGLPKLMSTRDVIRYATTEGARVAGLSGVTGSIEPGKQ
                     ADIIVLRTDRPNIFPVNDPIGAVVWGMDTSNLDWVFVGGRVLMREGVLEADVARARDL
                     ASRARQRVAVASGPVPGGATGGEG"
     gene            902..1951
                     /locus_tag="VF097_03475"
     CDS             902..1951
                     /locus_tag="VF097_03475"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6261890.1"
                     /translation="MKTERRSPSSAVTTFVVASRYMPVYLAILVLVIVAWIWAPATLG
                     APGLRAIAPFGTFLAIAALGQMLVVMTGGIDLSVPGSFTLAAVVTVGVGRASDEHLWP
                     AIGTALLMVALVGLVNGILIGGLGLNALIVTLAVGQIVSGIAYRYYTGVAIQTPVPPA
                     LAEWTSGRFLGVTRTFWVSVVLTVALIVIFRFTTVGRRFQVVGANPTAAWIAGLRVNL
                     NQVAAYVVAAVLYGAAGILLAAFLGTPSITIGAPYLLGPIAAVVIGGASLTGGLGSPL
                     STWAAAFFLAGLNQMMRVMGLPTALQFVVFGLVIIGGMLVSGDRIIRGVEHLLRERRR
                     SDPVEVPTIGPTGEA"
BASE COUNT          268 a          700 c          776 g          355 t
ORIGIN      
        1 cacacaccga cgccgggctg cgcaccgtgt tcgtccaggt ccccggagac ggggacgatg
       61 aggatgccct cctggagggg ctcgtccgtc tcgccccccg cgcgggaccg tcgacctcca
      121 tcgcgctcgg ttccgcggag ccgttcgacg cagaccgcct cgctcgccga tgggcggtgg
      181 cccgggagct cggcctgcgc atccacggtc acctggcgtc cggatccgcg gctggcacag
      241 tggcccgcct ggcgagccgg ggtcttctgg gcgacgacgt cacgctggtc cactgctccg
      301 gcctggacga gaaggacctc gacgcggtcg ccgactccgg cgcgtcggtc tcgctgtccc
      361 cctcgagcga catggccgag ggctccgggt ccccgccgat ccagcagctg atcgatcacg
      421 gtatcaggcc ggggctgggg gtcgatcacg agcgcctggc tccgggcgac gccttcgcgc
      481 agatgcgggc tacgatctcg ctccagcacg cgaccgtctt cgacctcaag ctggcgggga
      541 aggccggtct ccccaagctg atgagcaccc gggacgtgat ccgatatgcc accaccgagg
      601 gcgctcgggt cgcggggctg agcggggtga ccggctcgat cgagccgggc aagcaggcgg
      661 acatcatcgt gctgcgtacc gaccggccca acatcttccc ggtgaacgac ccgatcggcg
      721 ccgtggtctg gggcatggac acgtccaacc tcgactgggt cttcgtcggc ggccgcgtgc
      781 tgatgcggga gggcgttctg gaagccgacg tggcgagggc gcgtgatctg gcctccaggg
      841 cgcgacagcg ggtggccgtc gcgtccggcc cggtcccggg aggagcgacc gggggcgagg
      901 ggtgaagacc gagcgccgct ctccgtcgtc ggcggtgacc accttcgtgg tggcctcgcg
      961 ctacatgccc gtgtacctgg cgatcctcgt cctcgtcatc gtggcgtgga tctgggcgcc
     1021 cgcgacgctc ggggcgcccg ggctgcgggc catcgctccc ttcggcacgt tcctcgccat
     1081 cgcggcgctc ggtcagatgc tcgtggtcat gaccgggggg atcgacctca gcgtgcccgg
     1141 gtccttcacc ctggccgcgg tggtcaccgt gggggtgggt cgggcgtccg acgagcatct
     1201 ctggccggcg atcggcacgg cgctcctgat ggtggcgctg gtggggctcg tcaacggcat
     1261 cctgatcggg ggcctggggc tcaacgcgct gatcgtcacg ctggcggtcg ggcagatcgt
     1321 gagcgggatc gcctaccgct actacacggg ggtggccatc cagacgcccg tccctccagc
     1381 gctcgccgag tggacgtctg gacggttcct cggcgtgacc agaacgttct gggtgtcggt
     1441 ggtcctgacg gtcgccctca tcgtgatctt caggttcacg acggtcggcc gcaggttcca
     1501 ggtcgtcggg gccaatccca cggcggcctg gatcgccggt ctccgggtca acctgaacca
     1561 ggtggcggcc tacgtggtgg cggccgtcct gtacggggct gcggggatcc tcctcgcggc
     1621 gttccttggg acgcccagca tcacgatcgg cgctccctat ctgctcggcc cgatcgccgc
     1681 cgtggtcatc ggcggggcct cgctgacggg gggcctcggc agtccgctgt cgacctgggc
     1741 agcggcgttc ttcctggccg ggctcaacca gatgatgcgg gtcatggggc ttcccacggc
     1801 cctgcagttc gtcgtcttcg ggctcgtgat catcgggggg atgttggttt cgggggatcg
     1861 catcatccgc ggcgtggagc acctgctgcg ggagcgcagg cgatccgatc ccgtggaggt
     1921 accgaccatc ggtcccacgg gcgaggcgta gaaaggagga cgagatcaac gacccaggag
     1981 ttggactgga tgcatccaga ggggggagga gtatgaggcg gatcaagttc ttggggatcc
     2041 tcgtggtcct tgtgcttgtc gcggcggcgt gcggcgacga cggagacgac ggcgccaca
//
LOCUS       DASUTZ010000076         4960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_538783, whole genome shotgun sequence.
ACCESSION   DASUTZ010000076 DASUTZ010000000
VERSION     DASUTZ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538783"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VF097_03480"
     CDS             <1..209
                     /locus_tag="VF097_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEX6261891.1"
                     /translation="AYPELWRLAEDLLVHDELFALWRQQHVTMVERQIGSKTGTGGSA
                     GVSYLKATLDRRFYPELWAVRDDL"
     gene            223..1365
                     /locus_tag="VF097_03485"
     CDS             223..1365
                     /locus_tag="VF097_03485"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX6261892.1"
                     /translation="MDRFEAYRAEFPVVGEKAYLISASLGPVSGRAKTELAEYVKAWA
                     EMGAPEPVWFEVVFPKMRQAKRIFADLVGADPDELAVTTNVSMALSTITSCMDLSGER
                     NRIVTTELDFPTDHHVWRAQERLGAEVVVLESEDGLTVPLEAFDEAIDERTALVCINR
                     VLYRSSALLDAKEICRMARDAGAWSVVDDFHGAGIIPVDVHDLGCDFYTTGVLKWLCG
                     GPGLTFLYARRELIPSLEPTVTGWWGQAEPFGFTLELDRHPTARRLETGTHPAPVAFI
                     AHGGLEIISEVGPAAIRERQGELTDALIEGADTEGLPVRTPRDRDARGGVVNLGVGTE
                     AAKVAEALYERDVCVDSRGDGIRVSPHFFNTTSDIDTLFEQLREIL"
     gene            1365..1568
                     /locus_tag="VF097_03490"
     CDS             1365..1568
                     /locus_tag="VF097_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018518417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEX6261893.1"
                     /translation="MCRSIKRLREPHGGPRAPDEEVREAALQFVRKVSGFRQPSQRNA
                     EAFDAAVDEVAEASRRLLDAVTR"
     gene            1614..2762
                     /locus_tag="VF097_03495"
     CDS             1614..2762
                     /locus_tag="VF097_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX6261894.1"
                     /translation="MTRPEIPMTPGPTAVPPEVYLAQGSPLVYHRGPGYGELLREVTA
                     GLKALLKTESDVLVFTSSGTGGLESAVANLFSPGDRVVVPVTGYFGDRFAKIAEAFHL
                     DVRRVEYDWGRAVDPADVEEALGEAPTKGVLFQHSETSTGVINDVEPIARAAKDAGAL
                     TALDVISSAGAVPLETDAWGIDVAIGGSQKALSATPGIAFASISEAAWAAHRESTNPR
                     FYFDWTAYRGAYERENPEAPWTPAISLMLGLREALRLYLEEGAEAVLRRHELLSTATK
                     EGVRALGLDLFGEHPQRAWAVTTVRVPDGVDGKAIPAKMRADHGIIIAGGQGPLAGKV
                     FRIGHLGYYDRFDIVRCLAALELTLMDLGYPAKPGAAVAAAEEVFARA"
     gene            2759..4336
                     /gene="serA"
                     /locus_tag="VF097_03500"
     CDS             2759..4336
                     /gene="serA"
                     /locus_tag="VF097_03500"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX6261895.1"
                     /translation="MRVLVTETLAERGLSLLRESFEVDVRTELASEGLASAIAPYDGL
                     IVRSQTKVDAPVLEAAERLKVVGRAGVGLDNVDVEAATRRGVMVVNAPQSNVLSAAEH
                     TIALLLAQARNIPQAHAALRAGDWDRSRFQGMELNGKTLGILGLGRVGTLVAQRALAF
                     GMRLIAFDPFVPKDRARQLGIELMPDLGALLVQADVVTIHLPRIPETEGLLGERELRM
                     MKPGARLVNTARGGIVDEAALAAALESGHLAGAALDVFDEEPVTSHPLFRLDNVVVTP
                     HLGAATKEAQDKAGTSIAEMVKLALEGEFVPYAVNVAAAEVSELTRPFLPLAERLGKV
                     LQGLAEEAVGAVHVEYQGRVAEHDTSLLTLSVLKGLLSGVVHEPVSFVNAPIIARDRG
                     IQISDTRRLEAEDYVSLVAVSAETEAGAVQVAGTLAGPRGAERIVRVYDFDDVEMAPA
                     EHMAFFLYEDRPGVIGTVGTILGNAGINIAGMEVGRKEAGGLALMGLAVDGPIPEDVL
                     ERIVREAKMQRARSIRL"
     gene            4400..>4960
                     /gene="ilvE"
                     /locus_tag="VF097_03505"
     CDS             4400..>4960
                     /gene="ilvE"
                     /locus_tag="VF097_03505"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HEX6261896.1"
                     /translation="MPITPVEKVWMDGELIDWDDARIHVLTHALHYGTGVFEGIRAYA
                     TKQGPGIFRLTDHIRRLTHSASVYLMEVPYTPEELIEACKEVVRANDLEACYVRPVVY
                     RGYGEMGLNPLNAPVNTFIVTWRWGTYLGDEALQNGARVMISSWRRIDSNALPSGAKG
                     TGQYVNSSLAKIEALKAGYDDAIMLSP"
BASE COUNT          714 a         1735 c         1797 g          714 t
ORIGIN      
        1 acgcctaccc cgagctgtgg cgcctggccg aggacctcct cgtccacgac gagctcttcg
       61 cgctgtggcg ccagcagcac gtgacgatgg tcgagcgcca gatcgggtcg aagacgggca
      121 ccgggggatc ggccggggtc agctacctga aggccacgct cgaccgccgt ttctaccccg
      181 agctctgggc cgtgcgcgac gatctgtgag aggctgagcg ccgtggaccg gttcgaggcg
      241 taccgcgccg agttccccgt cgtgggcgag aaggcctacc tgatcagcgc gtcgctcggc
      301 cccgtctcgg ggcgggccaa gaccgagttg gccgagtacg tgaaggcctg ggccgagatg
      361 ggagcccccg agcccgtgtg gttcgaggtc gtcttcccca agatgcggca ggcgaagcgg
      421 atcttcgccg atctggtcgg cgccgacccc gacgagctcg ccgtcaccac gaacgtctcg
      481 atggcgctgt cgacgatcac gtcctgcatg gacctctcgg gggagcggaa ccgcatcgtc
      541 acgaccgagc tcgacttccc caccgaccat catgtgtggc gggcccagga gcggctgggg
      601 gccgaggtgg tcgtgctgga gagcgaggac gggctcaccg tccccctgga ggccttcgac
      661 gaggcgatcg acgagcgcac cgccctcgtc tgcatcaacc gggtcctcta ccggtccagc
      721 gccctgcttg atgcgaagga gatctgccgg atggctcggg acgccggcgc gtggtcggtg
      781 gtcgacgact tccacggcgc gggcatcatc cccgtcgacg tccacgacct cggctgcgac
      841 ttctacacca cgggcgtcct gaagtggctg tgcggtggtc ccgggctcac gttcctctac
      901 gcgcgccgcg agctgatccc gtcgctggag cccacggtca ccggctggtg gggacaggcc
      961 gagccgttcg gcttcacgct ggagctggac cgtcatccca cggcgcgccg gctggagacg
     1021 gggacccacc cggcaccggt cgcgttcatc gcccacggcg gcctggagat catctccgag
     1081 gtggggccgg ccgcgatccg cgagcggcag ggcgagctga ccgacgcgct gatcgagggc
     1141 gcggacaccg agggcctccc cgttcgcacg ccccgggatc gggacgcacg gggaggcgtg
     1201 gtcaacctcg gcgtggggac cgaggcggcc aaggtggccg aggcgcttta cgagcgcgac
     1261 gtctgcgtgg actcccgcgg tgacgggatc cgcgtctcgc cccacttctt caacacgacc
     1321 tcggacatcg acacgctgtt cgagcagctc agggagatcc tctgatgtgc cggagcatca
     1381 agcggctgcg cgagccgcac ggtgggcccc gcgcccccga cgaggaggtc cgcgaggcgg
     1441 ccctccaatt cgtccggaag gtgagcggct tccgacagcc gtcgcagcgg aacgccgagg
     1501 cgttcgacgc ggcggtggat gaggtcgcgg aggcctcgcg ccggctgctc gacgccgtca
     1561 cccgctgacg acgggtgcta cgctcgatgc tcccgagcct gtggagcgtg cgcatgaccc
     1621 ggcctgagat tcccatgacc cccggcccca ccgccgtccc tcccgaggtc tacctggcgc
     1681 aggggagccc cctcgtctac caccggggcc ccggctacgg cgagctcctc cgcgaggtga
     1741 ccgccgggct gaaggccctc ctcaagaccg agtccgacgt cctcgtgttc acctcgtcgg
     1801 ggactggagg gctcgagtcc gcagtggcca acctcttctc tccgggcgac cgcgtcgtgg
     1861 tcccggtgac cggctacttc ggcgaccgct tcgccaagat cgccgaagcc ttccacctgg
     1921 acgtccgtcg ggtcgagtac gactggggcc gcgccgtcga ccccgcggac gtggaggagg
     1981 ccctgggcga ggcccccacg aagggcgtgc tgttccagca ctccgagacc tcgacgggcg
     2041 tgatcaacga cgtggagccg atcgccaggg cggcgaagga tgccggcgcc ctgacggcgc
     2101 tcgacgtgat ctccagcgcc ggggccgtgc cgctcgagac cgacgcctgg ggcatcgacg
     2161 tggcgatcgg cggctcgcag aaggcgctct cggcgacgcc ggggatcgcc ttcgcctcga
     2221 tctcggaggc cgcctgggcc gcccaccgcg agtcgacgaa cccgcgcttc tacttcgact
     2281 ggaccgcgta caggggcgcc tacgagcgtg agaacccgga ggctccgtgg accccggcca
     2341 tctcgctgat gctcggcctg cgcgaggcgc tgcggctcta cctcgaggag ggggccgagg
     2401 cggtgctccg ccggcacgag ctgctctcga cggcgacgaa ggagggcgtg cgggccctcg
     2461 gcctggacct gttcggggag catccccagc gagcctgggc cgtcacgacc gtgcgcgtcc
     2521 ccgacggcgt ggacgggaag gcgatccccg cgaagatgcg ggccgaccac gggatcatca
     2581 tcgcgggcgg ccaggggccg ctggccggca aggtgttccg catcggccac ctcggctact
     2641 acgatcgctt cgatatcgtg cggtgcctgg ccgcgctgga gctcaccctc atggacctcg
     2701 gctatccggc gaagccgggg gcggcggttg ccgccgccga ggaggtcttc gcgcgggcgt
     2761 gagggtcctc gtcaccgaga cgctggccga gcgggggctg tcgctcctcc gggagtcgtt
     2821 cgaggtggac gtccgcaccg agctcgcctc cgaggggctc gcctctgcga tcgcgcccta
     2881 cgacggcctc atcgtccgct cccagaccaa ggtcgacgcc ccggtgctgg aggccgccga
     2941 gcgcctgaag gtcgtgggcc gggccggcgt cggcctcgac aacgtggacg tcgaggcagc
     3001 caccaggcgc ggcgtgatgg tcgtcaacgc gccgcagtcg aacgtgctct cggcggccga
     3061 gcacacgatc gcgctgctgc tcgcacaggc ccggaacatc ccgcaggcgc acgcggcgct
     3121 ccgggcgggc gactgggacc gcagccggtt ccaggggatg gagctgaacg gcaagacgct
     3181 ggggatcctg gggctcggtc gagtggggac cctcgtcgcc cagcgcgccc tggccttcgg
     3241 catgcggctg atcgcgttcg atccgttcgt gcccaaggat cgggcccggc agctggggat
     3301 cgagctgatg ccggatctcg gcgcgctcct ggtgcaggcc gacgtggtca cgatccacct
     3361 cccgaggatc cccgagaccg aggggctgct gggggagcgc gagctgcgga tgatgaagcc
     3421 gggcgctcgg ctggtcaaca cggcccgcgg cgggatcgtg gacgaggccg ccctcgcggc
     3481 ggccctggag agcggccatc tggcgggcgc ggcgctcgac gtcttcgacg aggagccggt
     3541 cacctcccat ccgctcttcc ggttggacaa cgtcgtggtg acgccgcacc tgggcgccgc
     3601 caccaaggag gcccaggaca aggcggggac ctcgatcgcc gagatggtga agctggcgct
     3661 ggagggcgag ttcgtcccct acgcggtcaa cgtggccgcg gccgaggtct ccgagctcac
     3721 ccgccccttc ctcccgctcg ccgagcggct ggggaaggtc ctccagggcc tggccgagga
     3781 ggccgtcggc gccgttcacg tcgagtacca ggggcgggtg gcggagcacg acacgagcct
     3841 gctcacgctc tcggtgctga aggggctgct gtcgggggtc gtccacgagc ccgtctcgtt
     3901 cgtcaacgcg ccgatcatcg cccgggatcg cggcatccag atcagcgaca cccgccggct
     3961 cgaggccgag gactacgtca gcctcgtcgc cgtctcggcc gagaccgagg cgggcgctgt
     4021 tcaggtggcc gggacccttg cggggccccg gggcgccgag cggatcgtcc gcgtctacga
     4081 cttcgacgac gtggagatgg ccccggccga gcacatggcg ttctttctgt acgaggaccg
     4141 cccgggggtc atcggcacgg tggggacgat cctcgggaac gccgggatca acatcgccgg
     4201 gatggaggta ggtcggaagg aggcgggggg cctggcgctg atggggctcg ccgtggacgg
     4261 cccgatcccc gaggacgtcc tggagcgcat cgtccgcgag gcgaagatgc agcgggcccg
     4321 ctcgatccgc ctctgacggt ggtcggcccc ccggggcgcc atgtaccctg agggcacccg
     4381 aagccaggga ggactgcgga tgcccatcac gccggtcgag aaggtctgga tggacgggga
     4441 gctcatcgac tgggacgacg ccaggatcca cgtcctcacc cacgccctcc actacggcac
     4501 cggcgtcttc gaggggatcc gcgcctacgc cacgaagcag ggacccggca tcttccgtct
     4561 caccgaccac atccgtcggc tcacgcactc ggcctccgtc tacctgatgg aggtgccgta
     4621 cacgcccgag gagctgatcg aggcgtgcaa ggaggtcgtc cgggccaacg acctcgaggc
     4681 ctgctacgtc cgcccggtcg tctaccgcgg ctacggggag atggggctca acccgctgaa
     4741 cgctccggtc aacaccttca tcgtcacctg gcgctggggg acctacctgg gggacgaggc
     4801 cctgcagaac ggcgcccgcg tcatgatctc gtcctggcga cggatcgact cgaacgccct
     4861 cccgtcgggc gcgaagggca cggggcagta cgtgaactcc tcgctggcca agatcgaggc
     4921 gctgaaggcc gggtacgacg acgcgatcat gctctcgccg
//
LOCUS       DASUTZ010000077         7998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_20967, whole genome shotgun sequence.
ACCESSION   DASUTZ010000077 DASUTZ010000000
VERSION     DASUTZ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7998
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20967"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..1319)
                     /locus_tag="VF097_03510"
     CDS             complement(210..1319)
                     /locus_tag="VF097_03510"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015231336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HEX6261897.1"
                     /translation="MTSFLRNPLREDFDPGPEPDPRPFHRKLPGYEPSPLREAPSIAE
                     RLGVGKVWVKDESSRLDLPAFKILGASWATYRALVRRLGEEPEWETIDELAERFEPLK
                     PLALATATDGNHGRAVARMARWLGLEARILVPAGTVPARIEAIEGEGAEVEVVPGTYD
                     DALRRAAELQSRAVEVVPDTAITGDEEAPKDASEGYATIFRELDEQLPAPPDLLPVQI
                     GSGALSSAAVRHYRPGGPRILGVEAEASDCVRASVLAGEMVSLPGPQTSIMAGLNCGT
                     PSATAWPLISRGIDAFVVVSDERAREAMRLLAESGIESGETGASGLAGLLEAFTGSEE
                     ALDAREGLRVGRATRVLVLSTEGATDPEAYRTIVG"
     gene            complement(1319..3043)
                     /gene="ade"
                     /locus_tag="VF097_03515"
     CDS             complement(1319..3043)
                     /gene="ade"
                     /locus_tag="VF097_03515"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEX6261898.1"
                     /translation="MTAFTDRLARRLAVARGDEPADLLLKGGRVLSVFTGELLEANVA
                     IAGEHVAGVGDYSDAAEVVDCSGLILLPGFIDAHMHLESTKLMVDQFARAALPWGTTT
                     VILDPHEIANVFGLDGVRAILASAGAVPLDFYVMVSSCVPASAFESSGATVTAEDIAR
                     FLAEEPSAIGVAEMMDYPSVVGGAPEALAKIEAAGSGHVDGHAPGLSGRELNAYLAAG
                     VGSDHEATTLDEAMEKRRLGMWILIREGSAARNLEALAPMVMEHGPTNCCLCTDDREP
                     DQLLREGHINDVMRKAVALGIRPADAVVMGTLNAARYHRLREHGAVAPGYLADVVAVE
                     DLEAFRPVRVWKRGRLVAEDGEAVGIAEVDAPDWMRGSVRVPSLTADDFSVSADGRVR
                     AIGVRDGSIVTDALVVDPRTEDGHAVSDVDRDLAKVAVVERHRETGRIGLGFVRGFGL
                     RRGALASTHAHDAHNVVVVGVDDADMAAAVNRLVEIGGGQVAVAGGEVVAEVPCPIGG
                     LLSDLRAEEVAAAFEGLERSAHELGATLASPFMAMSFLALSVIPELKITDRGLVDTVS
                     FELVPLEV"
     gene            3159..4625
                     /locus_tag="VF097_03520"
     CDS             3159..4625
                     /locus_tag="VF097_03520"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HEX6261899.1"
                     /translation="MTRTVLRLLLLGALCTSFLVPAGTAAAGTFRVTILGDPAPGACD
                     ARCSLREAVIAANASPGPDVIRLPKGRVELSKAGAGEEAGATGDLDVTDTLTIVGEGM
                     GRSVVDGNDLDRVFDASSGTFVLRRLTVTGGHVETGGATAFGGGIRGWGALVRLREVA
                     VRGNEAADGGGGVAGFTVEVHRSLIAGNIAHTSAGAVDGSTLKITDSLLLGNRATFSG
                     GAIMLFFGATADLRNTTVVGNTSGNSAGGIGINSGSALLLNVTIHDNHTDGDAGAPGG
                     NGGGVRLNTGATMEIRNTIISGNTDGAPTAAHDCAGDLTSLGSSLVRDQNGCSLTGAS
                     GDLPAGTDPGLRPPANNGGPTRTLALPPGSPARNAGEACLPADQRGVPRSLGGPCDIG
                     AYELVSCFGRVVNRVGTQGNDVLRGTGRKDGFLGFGGRDRLIGRGGSDAMCGNGGRDT
                     LEGGPGRDRLHGGPGRDLCIGGPGRDRARSCERRRQIP"
     gene            4690..6654
                     /gene="acs"
                     /locus_tag="VF097_03525"
     CDS             4690..6654
                     /gene="acs"
                     /locus_tag="VF097_03525"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX6261900.1"
                     /translation="MATEDRKDIAALLQEHRLIEAPPGFREQAVVSDEGIYARAADDP
                     EGFWAEQANHLDWIEPWDEVLRWDPPWVRWFDGGRLNISANCLDRHLEAHGDRVAYHW
                     EGEPGDERTITYRDLHEEVSRFANALKRIGVAKGDRVAIYLGMIPELPVAMLACARIG
                     AVHSVVFGGFSTDSLRDRINDAQAKVLVTADGGWRRGQTVPLKENADHAVAETPSIER
                     VVVVHRTGEEVPFREGRDLRYDELIAAESAECPAEPMEAEDPLYILYTSGTTGKPKGI
                     QHTTAGYLVGVTATHRTIFDIHPETDVYWCAADIGWVTGHSYIVYGPLANGTTSVMYE
                     GAPDFPGKDRLWSIVAKHDVTIFYTAPTAIRAFMKWGPDHARKHDLSSLRLLGTVGEP
                     INPEAWMWYWEHIGGERCPVVDTWWQTETGMILITPLPGITPLKPGSATRPFPGIEAD
                     VYDEAGNSVPSGAGGYLVLKRPWPAMARTIHGDPDRYVEQYFSKYGPSVYLAGDGARR
                     DEDGYFWLLGRIDDVMNVAGHRLSTYEIESALVDHPTVAESAVVSRPDPDVGEAIVAY
                     VTLKGHVEGDESLIAELREHVAEKIGKIARPHAIVLTDDLPKTRSGKIMRRLLRDVAH
                     GRDLGDVTTLANAEVVEEIRDLATSRSPEE"
     gene            6651..7859
                     /locus_tag="VF097_03530"
     CDS             6651..7859
                     /locus_tag="VF097_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6261901.1"
                     /translation="MTEAPGRRRALALLTLAMVLGLATWFSASAVIPQLREEWSLGPT
                     AAAWLTIAVQLGFVAGALVSAVLNLADVHSARTVIVVSGFGAALANLGLVAASDATLG
                     IPLRFLTGFFLAGVYPPGMKVIATWYREGRGYALGVLVAGITLGSAMPHLINGLGGLS
                     WRTVIVVTSVLTASGGAIARWMVDDGPFPFPRAVFDPRQAGRVFRNRGVRLATFGYFG
                     HMWELFAMWAWFVVFLTESLEAAGRDATVFAPLATFAVIGSGAIGSWIAGILGDRWGR
                     SRTTSAAMAISGASALTIGLLFERSPWLILAVGIVWGITVVADSAQFSAAVTETANTA
                     YVGTAVTLQTALGFTLTVATIWLVPIVRDALSWWWAFAMLAIGPALGILAMQRLQRSP
                     EAFRVAGGRG"
BASE COUNT         1130 a         2850 c         2887 g         1131 t
ORIGIN      
        1 atctcggttc gcaggttgga gcctcgctcc gagatcttga agtagcggtc gagcccggat
       61 acggcgccgg tctggacgcc tgagggcttc gtggtggcca ctgcgcctcc ctccttcgtt
      121 gccgagggcg gctcgcgggc cagccgccca ctccgcgcgg ggaaggcaac acgaagcata
      181 ggaatccggt ggggtggggc gcaagcggat cagccgacga tcgtgcggta ggcttcgggg
      241 tcggtcgcgc cctcggtgga taggaccagc acccgcgtgg cgcgaccgac ccggaggccc
      301 tcccgggcgt cgagcgcctc ctcggatccg gtgaaggcct cgagtagccc tgccaggccc
      361 gacgcgcccg tctcccccga ctcgatcccc gactccgcga gcaggcgcat cgcctcccgg
      421 gcccgctcgt ccgagaccac gacgaaggcg tcgatccccc gcgagatcag cggccaggcc
      481 gtggccgagg gcgtcccgca gttgaggccg gccatgatcg aggtctgggg cccggggagg
      541 gacaccatct cgccggccag caccgaggcc cggacgcagt ccgaggcctc ggcctccacg
      601 cccaggatcc gcggcccgcc cggccggtag tggcggacgg ccgccgagga gagggcgccg
      661 gagccgatct gcacggggag cagatcgggc ggggccggaa gctgctcgtc gagctcccgg
      721 aagatcgtgg cgtagccctc gctcgcgtcc ttcggcgcct cctcgtcccc ggtgatcgcg
      781 gtgtcgggca cgacctcgac cgcgcgcgac tggagctcgg cggcccggcg cagggcgtcg
      841 tcgtacgtcc ccggcacgac ctcgacctca gctccctcgc cctcgatcgc ctcgatgcgc
      901 gcggggacgg tccccgccgg gacaaggatc cgagcctcga gccccagcca ccgcgccatc
      961 cgggcgacgg cccgcccgtg gttgccgtcg gtggccgtgg ccagggccag gggcttgagg
     1021 ggctcgaacc gctccgcgag ctcatcgatc gtctcccact cgggctcctc gccgagccgc
     1081 cggaccagcg cccggtacgt ggcccacgag gccccgagga tcttgaacgc tgggaggtcg
     1141 agacgactcg actcgtcctt gacccaaacc ttccccacgc ccagccgctc ggcgatcgag
     1201 ggcgcctcgc ggagcgggga gggctcgtac ccggggagct tccggtggaa cggtcgcggg
     1261 tcgggctccg ggccggggtc gaagtcctcg cggagggggt tcctcaggaa ggaggtcatc
     1321 agacctcgag cggcacgagc tcgaagctga ccgtatcgac gagcccccga tcggtgatct
     1381 tcagctcggg gatgaccgag agcgcgagga aggacatcgc catgaagggg gaggcgagcg
     1441 tcgcgccgag ctcgtgggcc gagcgctcca ggccctcgaa cgccgcggcg acctcctcgg
     1501 cccggaggtc cgagagcagc cccccgatgg ggcacggcac ctcggccacg acctcccctc
     1561 cggccacggc gacctgcccg ccgccgatct cgaccagccg gttcaccgcc gccgccatgt
     1621 cggcgtcgtc cacgcccacg accaccacgt tgtgggcgtc gtgggcgtgg gtcgaggcga
     1681 gcgccccgcg ccgcaggccg aagccccgca cgaagcccag gccgatgcgg cccgtctccc
     1741 tgtgccgctc cacgacggcc accttggcca ggtcccggtc gacgtcggac accgcgtggc
     1801 cgtcctcggt ccttggatcc acgacgagcg cgtccgtgac gatcgacccg tcgcggaccc
     1861 cgatggcccg gactcgaccg tccgcgctca cgctgaagtc gtcggccgtg aggctcggga
     1921 cgcggacgct gccccgcatc cagtcgggcg cgtcgacctc cgcgatcccc acggcctcgc
     1981 cgtcctcggc cacgagccgc cctcgcttcc acaccctgac cggtcggaac gcctcgaggt
     2041 cctccacggc cacgacgtcg gccaggtagc cgggcgccac ggccccgtgc tcgcgcaggc
     2101 ggtggtaccg ggcggcgttg agggttccca tgacgaccgc gtcggccggg cggatcccca
     2161 gcgccaccgc cttgcgcatc acgtcgttga tgtggccctc gcgcagaagc tgatccggct
     2221 cgcggtcgtc ggtgcacagg cagcagttcg tcgggccgtg ctccatgacc atcggggcca
     2281 gcgcctcgag gttccgcgcc gccgagccct ctcggatcag gatccacatc cccagccggc
     2341 gcttctccat cgcctcgtcc agggtcgtgg cctcgtggtc ggagccgaca cccgccgcga
     2401 ggtacgcgtt cagctcccgg ccggagagcc cgggggcgtg gccgtccacg tgcccggagc
     2461 cggccgcctc gatcttggcc agcgcctcgg gcgctccgcc caccaccgag gggtagtcca
     2521 tcatctcggc cacgccgatc gcgctcggct cctcggccag gaagcgggcg atgtcctccg
     2581 ccgtgaccgt ggccccgctc gactcgaagg cgctggccgg cacgcacgat gacaccatca
     2641 cgtagaagtc gaggggaacc gccccggcgg aggccaggat ggcccggacc ccgtccagcc
     2701 cgaacacgtt ggcgatctcg tgagggtcga ggatcaccgt ggtcgtcccc cacgggaggg
     2761 ccgcccgggc gaactggtcc accatcaact tcgtggactc gaggtgcatg tgggcgtcga
     2821 tgaatccggg gaggaggatc agccccgagc agtccacgac ctcggcggcg tccgagtagt
     2881 cgccgacgcc ggcgacgtgc tccccggcga tcgccacgtt cgcctcgagc agctccccgg
     2941 tgaagacgga gaggacccgg ccgcccttca ggaggaggtc cgccggctcg tcgccgcgcg
     3001 ccacggcgag gcgtcgagcg aggcggtcgg tgaaggccgt cacgatggcg gacagcgtag
     3061 ccgcgcggcg acgagccggc aacggccggg acccctaccc tccgaggcga tcgaacggta
     3121 aggtatcgcg caagtgtcgt cggcttcggg aggcccttat gactcgaacc gtccttcgcc
     3181 tcttgctcct cggcgcgttg tgcacctcct tcctcgtccc agcgggcacg gcggccgccg
     3241 gcaccttccg ggtgacgatc ctcggcgatc ccgcgccggg cgcctgcgac gccaggtgct
     3301 cgctccgcga ggccgtgatc gcggccaacg cgtctccggg gcccgatgtc atccgcctgc
     3361 cgaaggggcg cgtcgagctg tcgaaggccg gagcaggcga ggaggccggc gccacgggcg
     3421 atctggacgt caccgacacc ttgacgatcg tgggagaagg catggggcgg agcgtcgtgg
     3481 acgggaacga cctcgaccgt gtcttcgacg cgtcctcggg caccttcgtg ctgcgtcgcc
     3541 tgacggtgac tggaggccac gttgagacgg ggggcgcgac cgcgttcggc ggcggcatcc
     3601 gcggctgggg agcgctcgtg agactgcggg aggtcgcggt gcgaggcaac gaggcggccg
     3661 acgggggggg tggcgtcgcg gggttcacgg tcgaggtcca ccgcagcctg atcgccggca
     3721 acatagccca cacctccgcc ggggccgtcg acgggtcgac cttgaagatc acggattccc
     3781 tcctcctggg gaacagagcc accttctcgg ggggagcgat catgctgttc ttcggggcca
     3841 ccgcggatct ccggaacacc acggtcgtcg ggaacacgag cggtaacagc gcgggaggca
     3901 tcggcatcaa ctccgggagc gctctgctgc tcaacgtcac catccatgac aaccacaccg
     3961 acggcgacgc cggggctccg ggcgggaacg ggggcggcgt ccggttgaac actggggcga
     4021 ccatggagat ccggaacacg atcatctccg ggaacacgga cggcgctccc accgcagccc
     4081 acgactgtgc aggcgacctg acgtccctcg ggtcgagcct cgttcgcgac cagaacggct
     4141 gcagcctcac cggtgcctcg ggtgacctcc ccgcggggac ggaccccggt ctccgccccc
     4201 cggcgaacaa cggcggcccg accaggacgc tggcgctccc ccccgggagc cccgcccgga
     4261 acgccgggga ggcgtgcctc ccggcagatc agcgtggggt tcctcgctcg ctgggcggcc
     4321 cgtgcgacat cggggcatac gagctcgtgt cgtgcttcgg gcgggtggtg aacagagtgg
     4381 gcacgcaggg gaacgacgtc ctgcggggga cggggaggaa ggatgggttc ctcggcttcg
     4441 ggggccggga tcggctgatc ggccgcggcg gctcggacgc gatgtgcggc aacggcggcc
     4501 gggacaccct cgagggtgga ccggggaggg atcgactgca tgggggcccg ggaagggatc
     4561 tctgcatcgg cggccccggc cgcgatcgag cccgatcctg cgagcgccgt cgccagatcc
     4621 cgtgacgccg cgttgcccgc tgcgcgacgc gggtgttgac tgaggcgtcg tcgtccgagg
     4681 agggcgggcg tggccaccga ggatcgcaag gacatcgcag ctctgctcca ggagcaccgg
     4741 ctgatcgaag cgcccccggg cttccgcgag caggccgtgg tctcggacga agggatctac
     4801 gcccgcgcgg ccgacgaccc ggagggcttc tgggcggagc aggcgaacca cctcgactgg
     4861 atcgaaccgt gggacgaggt cctgcgctgg gatccgccct gggtgcggtg gttcgacggg
     4921 ggacgcctca acatctccgc gaactgcctc gaccgtcacc tggaggccca cggggaccgg
     4981 gtcgcctacc actgggaggg cgagccgggg gacgagcgca cgatcaccta ccgcgacctc
     5041 cacgaggagg tttcgcgctt cgccaacgcc ctcaagcgta tcggcgtggc caagggcgac
     5101 cgggtcgcga tctacctcgg gatgatcccc gagctccccg tggccatgct ggcctgcgcc
     5161 cggatcggcg ccgtgcactc ggtggtcttc gggggcttca gcaccgactc gctgcgcgac
     5221 cggatcaacg acgcccaggc caaggtgctg gtgacggccg acggcgggtg gcgacggggc
     5281 cagaccgtgc cgctcaagga gaacgccgat cacgccgtgg ccgagacgcc gtcgatcgag
     5341 cgggtcgtcg tcgtccaccg cacgggcgag gaggtgccgt tccgtgaggg gcgcgatctc
     5401 cggtacgacg agctgatcgc ggccgagagc gccgagtgcc ccgccgagcc gatggaggcc
     5461 gaggaccccc tctacatcct gtacacctcg gggaccaccg ggaagcccaa ggggatccag
     5521 cacacgaccg ccggctacct cgtcggcgtg accgccacgc atcgcacgat cttcgacatc
     5581 cacccggaga ccgacgtcta ctggtgcgca gccgacatcg gctgggtcac cgggcactcc
     5641 tacatcgtct acggcccgct ggcgaacgga accacgagcg tgatgtacga gggggcgccc
     5701 gacttcccgg gcaaggaccg cctctggtcg atcgtggcga agcacgacgt gacgatcttc
     5761 tacacggcgc ccacggcgat ccgtgccttc atgaagtggg ggccggacca cgcccggaaa
     5821 cacgacctct cgtcgctgcg tctgctgggg accgtcgggg agcccatcaa ccccgaggcc
     5881 tggatgtggt actgggagca catcggcggc gagcggtgcc cggtcgtcga cacctggtgg
     5941 cagacggaga cggggatgat cctcatcacg cccctgcccg ggatcacgcc tctgaagccc
     6001 ggctcggcga cgcgtccgtt cccggggatc gaggccgacg tctacgacga ggccgggaat
     6061 agcgtgccgt ccggggcggg aggctacctc gtcctgaagc ggccctggcc ggcgatggcc
     6121 cggacgatcc acggcgaccc cgaccgctat gtggagcagt acttctcgaa gtacggcccc
     6181 tccgtctact tggcgggcga tggggccagg cgcgacgagg acggctactt ctggcttctg
     6241 ggacggatcg acgacgtcat gaacgtggcc ggccaccgcc tctcgaccta cgagatcgag
     6301 tcggcgctcg tggatcaccc caccgtggcc gagtcggcgg tggtctcgcg ccccgacccg
     6361 gacgtgggcg aggccatcgt ggcctacgtc accctgaagg gtcacgtcga gggcgacgag
     6421 tccctgatcg ccgagctgcg cgagcacgtg gccgagaaga tcggaaagat cgcccggccc
     6481 cacgcgatcg tgctgaccga cgacctcccc aagacccgct cggggaagat catgcggcgg
     6541 ctcctgcggg acgtggccca cggccgggac ctgggggacg tgaccaccct ggccaacgcc
     6601 gaggtcgtgg aggagatccg cgacttggca acgtcgaggt cgcccgagga gtgacggaag
     6661 cccccggtcg gcgtcgggcc ctcgcgctgc tgacgctggc aatggtgctg ggcctggcca
     6721 cgtggttctc ggcctccgcg gtgatccccc agctccggga ggagtggtcg ctcgggccaa
     6781 ccgcggcggc ctggctcacg atcgcggtcc agctcgggtt cgtggccggc gctctcgtct
     6841 ccgccgtgct gaacctggcc gacgtccact cggctcgaac ggtgatcgtg gtctccggct
     6901 tcggcgccgc gctcgcgaac ctgggcctcg tcgctgcgag cgacgcgacc ctggggatcc
     6961 ctctccgatt cctgaccggc ttcttcctgg caggcgtcta cccgcccggg atgaaggtga
     7021 tcgccacctg gtaccgggag ggacgcggct acgcgctggg ggtgctcgtc gccggcatca
     7081 ccctgggctc cgccatgccg cacctcatca acggcctggg aggcctctcg tggcggacgg
     7141 tgatcgtcgt cacctcggtc ctcaccgcct cgggcggggc gatcgcccgg tggatggtcg
     7201 acgacgggcc cttccccttc ccccgcgccg tcttcgaccc gaggcaggcg gggcgcgtgt
     7261 tccggaaccg aggcgtgcgg ctggccacgt tcggctactt cgggcacatg tgggagctgt
     7321 tcgcgatgtg ggcgtggttc gtcgtcttcc tcacggagtc cctggaggcc gcgggccggg
     7381 acgcaaccgt cttcgcgccg ctggcgacct tcgcggtcat cggctcgggg gcgatcgggt
     7441 cttggatcgc cgggatcctg ggcgatcggt ggggacggtc ccggacgacg agtgcggcga
     7501 tggcgatcag cggggccagc gcgctgacga tcggcctgct gttcgagcga tcgccgtggc
     7561 tgatcttagc cgtcgggatc gtgtggggca tcaccgtggt ggccgactcg gcccagttct
     7621 cggcggcggt cacggagacg gcgaacaccg cctacgtggg cacggccgtc acgctccaga
     7681 cggcgctcgg cttcacgctc acggtggcca cgatctggct ggtgccgatc gtccgcgacg
     7741 ccctctcgtg gtggtgggcg ttcgccatgc tggcgatcgg ccccgcgctg gggatcctgg
     7801 cgatgcagcg gctgcagcgc tcaccggagg cgttccgggt cgcgggcggg cggggctgat
     7861 cggccccgcc cgcccgcgac cccctggtag catccgggaa aggtcccgac gaggagaggt
     7921 ccttcgtacc gcgcgaggat cctctcctct cgtcacactt cgggagtgat cccgctgacg
     7981 gtcagacggc ggttggac
//
LOCUS       DASUTZ010000078         5756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_149178, whole genome shotgun sequence.
ACCESSION   DASUTZ010000078 DASUTZ010000000
VERSION     DASUTZ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5756
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149178"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1105..1308
                     /locus_tag="VF097_03535"
     CDS             1105..1308
                     /locus_tag="VF097_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261902.1"
                     /translation="MRREAPCFLVEDLSEGRSELVYLQLTEDLHLVTDDDLRALKEYL
                     RPLLLPGRPGRTYPSDMPHRVIE"
     gene            1308..1640
                     /locus_tag="VF097_03540"
     CDS             1308..1640
                     /locus_tag="VF097_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261903.1"
                     /translation="MQVEYTPLFEPPDVECALYTDRVLSEQEQEDLQTVVLEWYEREV
                     GRYDAPIHNMSELGFDHDGLPTIEWWIDLGSANLDRLDELVHVMQGRLSSWGIEPVKL
                     VLGRRHGP"
     gene            complement(2266..5421)
                     /locus_tag="VF097_03545"
     CDS             complement(2266..5421)
                     /locus_tag="VF097_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6261904.1"
                     /translation="MTACQTCGAENADGARFCQACGSALTLEDAEARQRKIVTILFSD
                     VAGSTSLGERLDPEMLRRVMATYFDRMRAVLERHGGTVEKYIGDAIVAVFGIPELRED
                     DALRAVRAAEEMRAALKEVNRELGERWGVAIEARTGINTGEVLAEEARPDFPLTADAA
                     NLAARLEQAAEPGEILLGETTYGLVRDAVDAEPAGPLELKGKSVSQAAWRLLGVGPEA
                     RGIARRLDSPIVGRDRELALLQEVFDSAVQERACRLVTVVAPPGVGKSRLAAEVLERV
                     EGRATVLRGRCLPYGDGITYWPVAEVLREAAAITDADPPAEATAKIAALLGDTAEAAA
                     RAERLGSALGLTDAPAPPQEIFWAFRRLVETMARDRPVAIEFDDIHWGEPTFLDLLEY
                     LAGWSTGAPIFVLCETRPELFDTRPGWATGIEDATTVRLEPLDDDDSERLIENLLGPA
                     PLPDTARSRIRHTAEGNPLFVEEILRMLVDQGLLRRTDARWEAAGDLSSIAVPPTINA
                     LLAARLERLSREERAVIHRASVVGKVFWWDAVAELSPAPARSAVGSHLQSLVRRELVR
                     PEPSRAGGEDAFQFSHILIRDAAYAGVTKELRAELHERFAEWLERRPTAGVEPEELIG
                     YHLAEAYRYRTELGLLDDHTGDLARRAAELLGSAGERALDRSDIPAAISLLSRATDLF
                     EPNDVRRIELLIDLAQALYESGEGHRGRAALDDAHSRAREHGDERLAARARIGRLHTR
                     AEVERGHGEDREEAHRAIEVFARHGDHRGLARAWALIAWAEWRGRRAAEVRVVSERAL
                     EHARRAGSPRDVAASVYLLGAALAHVPGPVEDAARRAEEVIEEERGNREVEAYLVHGL
                     VHLRAWAGRFDEARRHADRYRSILLENGQEALACDASEAAADVELWAGDAEAAIRILT
                     ENQRRFEELGVDDPALFPFLARAFVAAGRFEEAEPWAVKAVEAHHGLWRPIGQAQLAL
                     VRAHQGRAEEAEALAREAAGAFEGTDFLIFRGQTLVALAGVLRALGRADEASQVAREA
                     IKLFEEKGAAALADHARRALS"
     gene            complement(5497..>5756)
                     /locus_tag="VF097_03550"
     CDS             complement(5497..>5756)
                     /locus_tag="VF097_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019588821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEX6261905.1"
                     /translation="CLGVVQRNADESVTWVHSYVSRDKKRTFCIYDAPSPEAIRKTAL
                     RNGSACPRSRRTFKTVPSSSHSGVAPLTVVAQRSTTARPSP"
BASE COUNT          890 a         1994 c         1927 g          945 t
ORIGIN      
        1 gtccctatag acgcggccta tcatcagctg atcaccaatg agttccggcg gctacatcct
       61 tatggcagcg cgaggccaac tccgcagaga ctctcggagc ttatgaggca ggtgtactcg
      121 aagtatcctc ttccttgacg cgggaagcac tcgtcttgag agacaggatc gaattccgaa
      181 ttccagagga gcatgctcgg aggtatctcc ccgaggatgt tgggactcga ttgggtgaca
      241 gcgtccggaa gctcgaggtg gaagtgcctt cccccctctt cgaacggata gggcagatcg
      301 atcgccacct tcgagcgcag ggccgggcct tcttcacggc ctgggcttct cggaggacct
      361 acacaaaggc tgagttggag gcagccgatc tactgcttct cacaatcaca acggtgctgg
      421 aacccgcggg agaagagtgc ggtacgaagt acgacgaggc gcgagcgtgt gagttctgtg
      481 gcgctggtcg gcgacaagtc tccactctcc atctcgatcc tagacgactc cctcggagca
      541 tgcgccttgc caggaccatc gcggatgaaa acctgatgcc tgcctcggtg tccgatcgtt
      601 tcgccaatga gagtctacga ggacttcgat ccctccccat caagggatgc tccccctcgg
      661 ccgagatcat tgagggatgg gtacaagtat cagtcacatc cgagcttgag atcggcccat
      721 cgactcgctt tggggaggat cccttcgatg agtcccagtc gtcgatctgt ccgatcgggc
      781 acatcgccgg gctgaaccta ctgtctcagg ttcaggtaat tggcggatca tgggacggaa
      841 gcgatctcgt acgttctcgg tccctagtgg gggatcgtcg agggctgctc gttccctatc
      901 cactcctatt cgtttctcag cgtgctcgtc gacttcttcg cagtatggga gtaagggggt
      961 ggcaggccga gcccgttcgc ttcgcgtaac gctcagctct caggatttga ggttgctcac
     1021 cttcgtggtc aactgagctc tacgcggaca cagccttctc cgacgccgac gtgatcgggt
     1081 caaggcctca tcgggatgga gcgcgtgcgc cgtgaggctc cgtgcttcct ggtcgaggat
     1141 ctctccgagg gccggagcga gctcgtctac ctccagctca ccgaggacct gcacctcgtc
     1201 accgacgatg acctccgcgc cctgaaggag tacctgcggc ccctcctcct gcccgggcgc
     1261 ccaggcagga cgtatccgtc cgacatgccc cacagggtga tcgagtgatg caggtcgagt
     1321 acacgccgct cttcgaaccg cccgacgttg agtgcgccct atacacggac agggtgctct
     1381 ccgagcaaga gcaggaggac ctgcagacgg tcgtcctcga gtggtacgag cgcgaggtgg
     1441 ggcgctatga cgcacctatc cacaacatgt cggagctagg attcgaccac gatggcctcc
     1501 ccaccatcga gtggtggatc gatctgggtt cggcgaacct cgaccgcctc gacgagctcg
     1561 tgcacgtgat gcagggacgc ttgtcgtctt ggggcatcga gcccgtcaag ctcgtgcttg
     1621 ggcggcgaca tggcccttag ggttgccgcg cgcttgccgg agggagatca gaggcctgag
     1681 ctcggtacga tctgttcgag ctgaacagta cggccgaaat cgcgaggctg gctggaacct
     1741 ccgccagccc tggtgccatc atgtgcagcc ctcgttgcct tctagtcctt gaactggaag
     1801 tggcactggc ctcggctttt gcccgagttt tgcccaagtg acgagacctc aggcgacccg
     1861 agccgacctc agatgacccg tgagcagcgg atatgcctca gagaacccgg gaatgcacga
     1921 ctcataaccc agaggtcgct ggttcaaatc cagcccgcgc aacgagagaa accgcaggta
     1981 gatggctcaa caggcggctc cggcgtccga ggggccgccc tcaagttggg tactcagcgt
     2041 gggcgatgtg cttcgttgag gacgcctccg tcgtcgtggt cctgaccaat cgagaggatg
     2101 tcaggatcga ggtcgccgat gccctggcag cgatggggct ccggggaagc cgaggatgat
     2161 tcctaccttc gcgtccagtg cgtcgaagtg gacgaagttg cgagtcccgc gcccacctga
     2221 cctcctccag ggtgacttga tcggaaggcg cgcctagccc tacgatcagg acagggcccg
     2281 gcgggcgtgg tcggccagcg ccgctgcacc cttctcctcg aacagcttga tggcttcccg
     2341 cgcgacctga ctcgcctcat cggcacgacc cagggcgcgg aggacgccgg ccagggccac
     2401 cagggtctgc ccccggaaga tcaggaagtc ggtcccctcg aaggcccccg cggcctcccg
     2461 ggccagggcc tcggcctcct cggcccggcc ctggtgggcc cggaccagcg ccagctgcgc
     2521 ctggccgatc ggtctccata ggccatggtg cgcctccacc gccttgacgg cccacggctc
     2581 cgcttcctcg aagcgcccgg cggcgacgaa ggccctggcc aggaagggga acagggcggg
     2641 atcgtccacc ccgagctcct cgaaccggcg ttggttctcg gtgaggatcc ggatggccgc
     2701 ctcggcgtcc ccggcccaca gctccacgtc ggcggcggcc tccgaggcgt cgcaggccag
     2761 ggcctcctgg ccgttctcca agaggatgga ccggtaccgg tcggcgtgtc gacgggcctc
     2821 gtcgaaccgt cccgcccacg cccggaggtg aacgaggccg tgaacaaggt aggcctccac
     2881 ctccctgttc cctcgctcct cctcgatgac ctcctccgcc cggcgggcgg cgtcctcaac
     2941 cggtccgggg acgtgggcca gcgccgcgcc taggaggtag acgctggctg ccacatcgcg
     3001 gggactcccc gcccggcggg cgtgctcaag ggcccgctcg gagaccactc tcacctcggc
     3061 ggcccggcgg ccccgccact cggcccacgc gatgagcgcc caggcacggg ccaggccgcg
     3121 gtggtctccg tgccgggcga agacctcgat tgcccgatgg gcctcctccc ggtcttcccc
     3181 atggccgcgc tcgacctcgg cgcgagtgtg caggcggccg atcctggccc tggccgccaa
     3241 gcgctcgtcg ccatgctccc gtgcccggga gtgggcatcg tccagcgccg cccggccccg
     3301 gtgcccctcc cccgactcgt aaagggcctg ggccaggtcg atcaggagct cgatgcgcct
     3361 cacgtcgttc ggctcgaata ggtcggtggc ccgggagagc agcgagatgg ccgctgggat
     3421 gtcgctcctg tccagggctc gctctcccgc cgaaccgagc agctccgcgg cccgtcgggc
     3481 cagatccccg gtgtggtcat cgaggaggcc gagctcggtc cggtagcgat aggcctcggc
     3541 caggtggtag ccgatcagct cctcgggctc cacgcccgcg gtgggccgac gctccagcca
     3601 ttcggcgaac cgctcgtgga gctccgccct gagctccttg gtcacccccg cgtaggcggc
     3661 gtccctgatc aggatgtggc taaactggaa ggcgtcctcg ccgccggccc gggacggctc
     3721 gggacggacc agctccctgc ggaccagcga ctggaggtgg ctcccgactg ccgagcgtgc
     3781 cggagcaggc gacagctcgg ccacggcgtc ccaccagaag accttgccga ccaccgaggc
     3841 ccggtggatc accgcccgct cctcgcggga gagacgttcc aaccgggccg ccaggagggc
     3901 gttgatcgtg ggggggacgg cgatcgagga caggtcgccg gcggcctccc acctcgcgtc
     3961 ggtgcgccgc aggagccctt ggtccaccag catccgcagg atctcctcca cgaacagcgg
     4021 gttcccttcc gccgtgtggc ggatcctcga ccgcgccgta tccgggagag gcgccgggcc
     4081 caggaggttc tcgatcagcc gctcgctgtc gtcgtcgtcg aggggctcca gccgcaccgt
     4141 ggtggcgtct tcgatccccg tggcccaccc gggccgggtg tcgaacagtt cggggcgggt
     4201 ctcgcagagg acgaagatgg gcgccccggt gctccacccg gccaggtact cgagcaggtc
     4261 gaggaacgtg ggctcccccc agtggatgtc gtcgaactcg atggccacgg gcctgtcccg
     4321 ggccatggtc tccaccagcc ggcggaacgc ccagaagatc tcctggggcg gcgccggggc
     4381 gtcggtcagg ccgagagcag agccgagccg ctcggccctc gccgcggcct ccgcggtgtc
     4441 cccgaggagg gcggcgatct tggccgtagc ctcggccggc gggtcggcat cggtgatggc
     4501 cgcggcttcc cgcaggacct cggccaccgg ccagtaggtg atcccgtccc cgtaggggag
     4561 gcaccggccc cggaggaccg tggcccggcc ctccactcgc tcgaggacct cggcggccag
     4621 gcgggacttc cccacgcccg gcggggcgac cacggtgacc agtcggcagg cgcgttcctg
     4681 gaccgccgag tcgaagacct cctggagaag ggccaactcc cggtcccggc ccacgatggg
     4741 ggagtcgagc cggcgggcga tcccgcgggc ctccgggccc acgcccagca ggcgccacgc
     4801 cgcctgggaa acgctcttgc ccttgagctc caggggaccc gcgggttccg cgtcgacggc
     4861 gtcgcggacc aggccgtacg tcgtctctcc cagcaggatc tcccccggct ccgccgcctg
     4921 ctccaggcgg gcggcgaggt tggcggcgtc cgcggtgagg gggaagtcgg ggcgggcctc
     4981 ctcggccagg acctccccgg tgttgatgcc ggtccgggcc tcgatggcca ccccccaccg
     5041 ctcgccgagc tcccggttga cctccttcag ggcggcgcgc atctcctcgg cggcccggac
     5101 cgcccggagg gcgtcgtcct cccgcagctc ggggatgccg aacacggcca cgatggcatc
     5161 gccgatgtac ttctccacgg tcccgccgtg ccgctcgagc accgcccgca tgcgatcgaa
     5221 gtaggtggcc atgacccggc ggagcatctc ggggtccagc cgctcgccga gcgaagtgga
     5281 cccggccacg tcggagaaca ggatggtgac gatcttgcgc tgccgggctt cggcatcctc
     5341 gagtgtcagc gccgagccgc acgcctggca gaaccgggca ccgtcggcgt tctccgcccc
     5401 gcacgtctga caggccgtca cgagcaggag gctaccccga ctcgatcggg ctaggttggt
     5461 gggacctatt ccgacgcggc accgagcaaa ccggtctcag ggggacggtc tcgccgttgt
     5521 ggaccgctga gccaccacgg tcagaggagc aacgccactg tgggacgagg aggggaccgt
     5581 cttgaaggtc ctgcggctcc gtggacaggc tgaaccattc cggagagccg tcttcctgat
     5641 cgcttcgggg ctgggcgcgt cgtagatgca gaacgtcctc ttcttgtccc ggctcacgta
     5701 agagtgaacc cacgtgacgc tctcgtccgc gttccgctgg acgactccga ggcata
//
LOCUS       DASUTZ010000079         5564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1576026, whole genome shotgun sequence.
ACCESSION   DASUTZ010000079 DASUTZ010000000
VERSION     DASUTZ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5564
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576026"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1411
                     /gene="gltX"
                     /locus_tag="VF097_03555"
     CDS             <1..1411
                     /gene="gltX"
                     /locus_tag="VF097_03555"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX6261906.1"
                     /translation="TVRVRFAPAPSGEMHVGNARTALFDWLFARHHGGVMILRIEDTD
                     QERVTDEAITGVQESLRWLGIDWDEGPGVGGPHGPYRQMERLDLYREMTDRLLEQGDA
                     YPCYCTPEELEERRKEALEGGERPGYDGRCRRLTDEERAAFEREGRPSAIRFATPDRD
                     VTFTDLVRGEATFRGPDVTDFVILRSSGLPTYLLAAAVDDWTMEMTHVIRGEDLFSST
                     PRQILLLEALGSERIPEYAHLPLIVGPDRAPLSKRHGSVAVERFREEGFLPEALVNYL
                     ALLGWSYDDHTTFFTRDELVERFDLSRVSHNPAAFDREKLEWMNGHYIRESSDERLAD
                     LITGVMKDEGVSADRDVVLEAVPLVKERMKLLSESVGLLRFLFEEPVPDDKARTQIEG
                     SEDYLRDVADRLEALGEWTAGTIEEVLRALKKERELGSKRAFQPVRAAATGTLVSPPL
                     FESLELLGRERTLARLRRA"
     gene            1413..1931
                     /locus_tag="VF097_03560"
     CDS             1413..1931
                     /locus_tag="VF097_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018788698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX6261907.1"
                     /translation="MGHVAVTAIGADRPGIVAGVTEAIRDLGGNLEDVASTILRGHFT
                     MMLVVDAPVPAADVEAALRDAVESLGVTVTAADVEAGAPGRPQATHALVVYGSDRPGI
                     VAGIARLLAERGVNVTNLSCRLVGEEQPVYAMVAEVQVPEGDEDLPRLVEDRARELEV
                     DVTLRPIEVETL"
     gene            1934..2434
                     /locus_tag="VF097_03565"
     CDS             1934..2434
                     /locus_tag="VF097_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX6261908.1"
                     /translation="MAVRPVVRYPDPILKRPAEVVGEPAVEHRRLADDLVDTMRAGPA
                     TVGLAAPQIGVPVRAFCLDVSGHRRATSSHGLVVLFDPVLVEVRAEDVLREGCLSVPD
                     LTANVRRAVRAVVRGSEPDGTRRVYQMEGFEARALLHELDHLDGLLILDRVDSAADVF
                     PRKVYG"
     gene            2486..5386
                     /locus_tag="VF097_03570"
     CDS             2486..5386
                     /locus_tag="VF097_03570"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX6261909.1"
                     /translation="MTPAGSAVPPGAQLCGRTRELRELVDALRRPPSLLVLEGEAGVG
                     KSRLVLELLGRPELEARRFLVGHCAAVAEPFPLGAIVEALRGLADGPSFPELSPVCGV
                     LRPLLPELSRVLPPPIEPGKDRRMRGHLVTRAVREVLGAVGELVLVLEDLHRADAQTI
                     EVLADLAASPPGRVSTLLTYRREELPEGSPLPELLAHLPPSAHRLHRTLGPLDSAGVR
                     CMIEHDLGTQEIPDGFVRGIFDSTGGLPFAVEELLGFLRDRSRSLEELLAESPRRFSE
                     VPLALRDAVFDDAPVAVRDSVLGRVGRLSRGARRIVEAAAIPEPPVETKLLQRVTRLG
                     EEAYARAVDEAVRAGLLEEAGAGGVKFRVAIARVALHRSMAPERHRALHLATARALAA
                     LDLRGPASIAYHYGEAESERQWARWAERAADQAVSVGDDAWAARMLDEVLSIATLPRE
                     ARVRLSVKFARAALYGLQQVEAIGVVRHIFHTETLTAPVRGELRLALARLLMEAGDAR
                     EGHMEASIAASELRGRPAEAEAEAFVASPWVEGISPGEHLAAEARAAAMLPTLADPEQ
                     RAHVEGDLMIARLLRGDPEAVEEIESLERTSLTIGVGRELQRTRISAALALMLLGAYD
                     RAAQALKEATVVTEDPSFARLTPYVLADEVLLDFLTGRWDGLLERAQALQHGGPPQAT
                     NKARLVIALLAMARGATARAEAILGELVPRILKAGGVPLAASALSAMSFARLARGHVG
                     GALEVARESLELIRFKEMWAVGGEAVSFFVSAALRAGRRSEAQAAVAELSDGIRGRFA
                     PGAHAALLTARALLLGEKGASAYRRAGRAWEALPRPLAVAACREGEGEALASTDGARA
                     RAALTESLAILERLGAGWHADRVRRKLRGSGVVVSRPWRGGRRGYGDELSPRELEVAR
                     LVAEGRTNRQIAERLYLSPRTVGHHVSNAMRKLGVTSRAALAVAATNGGGEE"
BASE COUNT          697 a         1996 c         2103 g          768 t
ORIGIN      
        1 gacggtccgt gtgcgcttcg cccccgcccc ttcgggcgag atgcacgtgg gcaacgcgcg
       61 caccgccctg ttcgactggc tgttcgcccg tcaccacggc ggcgtgatga tcctccggat
      121 cgaggacacc gaccaggagc gcgtcaccga cgaggcgatc accggcgtcc aggagtcgct
      181 ccgctggctg gggatcgact gggacgaggg gcccggggtg gggggtcccc acggcccgta
      241 ccgccagatg gagcggctgg acctgtaccg cgagatgacc gaccgcctgc tcgagcaggg
      301 cgacgcctac ccgtgctact gcacccccga ggagctggag gagcgcagga aggaggccct
      361 ggaggggggc gagcggccgg ggtacgacgg gcggtgccgg cggctcacgg acgaggagcg
      421 cgccgccttc gagcgagagg ggcggccgtc cgcgatccgc ttcgccacgc ccgatcgcga
      481 cgtgacgttc accgacctcg tccggggcga ggccacgttc cgcggccccg acgtcaccga
      541 cttcgtgatc ctccgcagca gcggcctgcc cacctacctc ctggccgcgg ccgtggacga
      601 ctggacgatg gagatgacgc acgtgatccg cggcgaagac ctcttctcgt ccaccccgcg
      661 gcagatcctg cttctcgagg cgctgggctc ggagcggatc cccgagtacg cgcacctgcc
      721 gctgatcgtg ggaccggatc gtgccccgct ctccaagcgc cacggctcgg tggcggtgga
      781 gcggttccgg gaggaggggt tcctccccga ggccctcgtg aactacctgg ccctgctcgg
      841 ctggtcctac gacgaccaca ccaccttctt cacccgggac gagctcgtgg agcggttcga
      901 cctgtcccgg gtcagccaca accccgcggc gttcgaccga gagaagctcg agtggatgaa
      961 cggccactac atccgggagt cgtccgacga acgcctggca gacctgatca ccggcgtgat
     1021 gaaggacgag ggggtgagcg ccgaccgcga cgtcgtcctg gaggccgtcc cgctggtcaa
     1081 ggagcggatg aagctgctga gcgagtcggt gggcctgctc cggttcctct tcgaagagcc
     1141 ggtgcccgac gacaaggcgc ggacgcagat cgaggggagc gaggactacc tgcgcgacgt
     1201 ggccgaccgg ctcgaggcgc tgggcgagtg gacggccggg acgatcgagg aggtgctccg
     1261 ggccctgaag aaggagcgcg agctggggtc gaagagggcg ttccagccgg tgcgggcggc
     1321 ggccaccggc acgctcgtgt cgcccccgct gttcgagtcg cttgagctgc tggggcggga
     1381 gcggacgctg gcccgcctgc ggagggcctg acgtggggca cgtggccgtc accgcgatcg
     1441 gggccgaccg gcccggcatc gtggccgggg tcaccgaggc gatccgcgac ctcgggggga
     1501 acctcgagga cgtggcctcc acgatcctgc ggggccactt cacgatgatg ctcgtggtgg
     1561 acgcgccggt gccggcggcc gacgtggagg cggccctccg ggacgccgtc gagtccctcg
     1621 gggtcacggt caccgcggcc gacgtcgagg ccggggcccc gggccgcccg caggccacgc
     1681 acgcgctcgt cgtctacggg tccgaccgtc cggggatcgt ggccggcatc gcccggctgc
     1741 tggccgagcg cggcgtgaac gtcacgaacc tctcgtgccg gctggtgggg gaggagcagc
     1801 ccgtctacgc catggtggcc gaggtccagg tgcccgaggg ggacgaggat ctcccccgcc
     1861 tcgtcgagga ccgggcccgg gagctcgagg tggacgtcac gctccgcccg atcgaggtcg
     1921 agaccctcta gcgttggccg tccgcccggt cgtccggtac cccgacccga tcctgaagcg
     1981 cccggcggag gtcgtcggcg agccggcggt ggagcatcgt cggctcgccg acgatctcgt
     2041 ggacaccatg cgggccggtc cggccacggt gggcctggcc gccccgcaga tcggggttcc
     2101 cgtgcgcgcc ttctgcctcg acgtctctgg ccaccggagg gcgaccagca gccacggcct
     2161 cgtcgtcctc ttcgacccgg tcctggtcga ggtgcgggcg gaggacgtgc tgcgtgaggg
     2221 ctgcctctcg gtccccgacc tcaccgcgaa cgtgcgtcgt gccgtccgcg ccgtggtccg
     2281 ggggagcgag ccggacggga cgcggcgcgt ctaccagatg gaggggttcg aggcccgggc
     2341 cctcctgcac gagctcgatc acctcgacgg gctcctgatc ctcgaccgcg tcgattccgc
     2401 ggcggacgtc ttcccccgga aggtgtacgg ataggaccgc gttgcccgcc ttcggatacg
     2461 atgtccggat gccgcggaga acgcgatgac gccggccggg tcggccgtcc cccccggggc
     2521 gcaactgtgc gggcggacac gagagctccg cgagctcgtc gacgcgctgc gtcgcccgcc
     2581 gtccctcttg gtgctcgagg gcgaggccgg ggtggggaag agccggctgg tgctcgagct
     2641 cctcggccgt ccagagctcg aggcgcggcg cttcctcgtg ggccactgcg ccgcggtggc
     2701 ggagccgttc cccctcgggg ccatcgtcga ggcgctgcgc ggcctcgccg atggcccctc
     2761 cttccccgag ctgagccccg tctgcggggt gctccgcccc ctgctccccg agctctcccg
     2821 cgtcctgccg ccgcccatcg agcccgggaa ggaccggcgg atgcggggcc acctcgtgac
     2881 ccgggccgtc cgcgaggtgc tgggtgcggt gggcgagctc gtgctggtcc tggaggacct
     2941 gcaccgggcc gacgctcaga cgatcgaggt gctggcggac ctggccgcct ccccgccggg
     3001 tcgcgtgtcg accctcctga cctaccgccg tgaggagctg cccgagggct cccccctccc
     3061 cgagctcctg gcccacctgc ctccttccgc ccaccggctc caccggacgc tcgggccgct
     3121 cgattcggcg ggggtgcgct gcatgatcga gcacgacctc ggaacccaag agatccccga
     3181 cggcttcgtc cggggcatct tcgacagcac gggcgggctc cccttcgcgg tcgaggagct
     3241 cctggggttc ctgcgggacc ggtcgagatc cctcgaggag ctgctggccg agtcgccccg
     3301 ccgcttctcc gaggtccctc tggccctccg ggatgcggtc ttcgacgacg ccccggtcgc
     3361 cgtccgggac tcggtcctcg gccgtgtcgg tcggctctcc cgcggcgccc gccgcatcgt
     3421 ggaggcggcc gcgatccccg agccgcccgt ggagaccaag ctgctgcagc gcgtcacgag
     3481 gctgggggag gaggcgtacg cccgtgcggt ggacgaggcc gtccgggccg gcctgctcga
     3541 ggaggcgggt gccggaggag tgaagttccg tgtggcgatc gcccgcgtag ccctgcaccg
     3601 gtccatggcc ccggagcgcc accgggccct gcatctcgcg accgctcggg ccctcgccgc
     3661 cctcgatctc cgcggcccgg caagcatcgc ctaccactac ggcgaggccg agtcggaacg
     3721 gcagtgggcc cggtgggccg agcgggcggc cgaccaggcc gtctccgtcg gcgacgacgc
     3781 gtgggcggcc cggatgctgg acgaggtcct ctcgatcgcg acgctccctc gggaggcgcg
     3841 cgtccgactg tcggtcaagt tcgcgcgggc cgccctctac ggtctccagc aggtggaggc
     3901 gatcggcgtg gtccgccaca tcttccacac cgagacgctg acggcgcccg tgcgcgggga
     3961 gctccgcctg gcgctggccc gcctgctcat ggaggcgggc gacgctcggg agggccacat
     4021 ggaggcgtcc atcgccgcct cggagctgcg gggccggccg gcggaagccg aggccgaggc
     4081 cttcgtggcg tcaccctggg tcgaggggat ctcgcccggc gagcacctcg ccgcggaggc
     4141 ccgggccgcc gcgatgctcc ccacgctggc ggatccggag cagcgggccc acgtggaggg
     4201 cgacctgatg atcgctcgcc tgctgcgggg tgatccggag gccgtggagg agatcgagag
     4261 cctcgagcgg acctccctca cgatcggggt ggggagggag ctccagcgca cccgcatcag
     4321 cgccgccctg gccctgatgc tcctgggcgc gtacgaccgc gccgcccagg ccctcaagga
     4381 ggcgacggtc gtcaccgagg acccctcctt cgctcgcctg accccgtacg tgctcgccga
     4441 cgaggtcctt ctcgacttcc tgacgggacg gtgggacggc ctcctggagc gggcgcaggc
     4501 gctccagcac ggggggccac ctcaggcgac gaacaaggca cgcctggtca tcgccctgct
     4561 ggcgatggcc cggggcgcca ccgcgagggc cgaggcgatc ctgggggagc tggtgcctcg
     4621 gatcctcaag gcgggagggg tgccgctggc cgcctcggcc ctctcggcga tgtcgttcgc
     4681 gcgcctcgcg cggggccacg tcggcggcgc gctggaggtc gctcgcgaga gtctcgagct
     4741 catccggttt aaggagatgt gggccgtggg gggagaggcc gtgtcgttct tcgtcagcgc
     4801 cgccctgcgc gccggtaggc ggagcgaggc ccaggccgcc gtggccgagc tgtccgacgg
     4861 catcagaggg aggttcgcgc cgggcgccca tgcggcactc ctcacggccc gcgccctcct
     4921 cctgggcgag aagggcgcct ccgcctaccg gcgcgcaggg cgggcatggg aggcgctgcc
     4981 gcggccgctg gccgtcgccg cctgcaggga aggggagggc gaggcgctgg cctctaccga
     5041 cggggcccgc gcccgggccg ctctgaccga gagcctggcg atcctggagc gcctgggggc
     5101 cggctggcac gccgatcggg tccgtcggaa gctccgcggg tccggcgtcg tcgtgtcccg
     5161 tccctggcga ggagggaggc gcggctacgg ggacgagctc tcgcctcggg agctcgaggt
     5221 ggcccggctc gtggcggagg gtcgcacgaa tcggcagatc gccgagaggt tgtacctgtc
     5281 ccctcggacg gtcgggcacc acgtctccaa cgcgatgcga aagctgggcg tcacctcccg
     5341 ggcagccttg gccgtggcgg ccacgaacgg cggaggggaa gaataggtca tccgcaccat
     5401 cgcgagcatc ctcccgcatg ggccatgctg atggtccggc gccgacggag ccgaggtgac
     5461 gctgtcagca gagcgggttg ggaacgtcgc aacgttgctc ccggtcggcg cccggaacga
     5521 cggagcgagg gcgggcgacg gcccgccctc ctccctcaaa cccc
//
LOCUS       DASUTZ010000080        10843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_21863, whole genome shotgun sequence.
ACCESSION   DASUTZ010000080 DASUTZ010000000
VERSION     DASUTZ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10843
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21863"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VF097_03575"
     CDS             <1..272
                     /locus_tag="VF097_03575"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006559656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase / HUMPS
                     family protein"
                     /protein_id="HEX6261910.1"
                     /translation="AFEASEAGARGVVCSGDHVREVRDALGEDPLIVVPGIRPGGMGA
                     NDHARTLTPREAVERGADLLVVGRPITQAADPAQAARAIARELEG"
     gene            269..817
                     /gene="pyrE"
                     /locus_tag="VF097_03580"
     CDS             269..817
                     /gene="pyrE"
                     /locus_tag="VF097_03580"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX6261911.1"
                     /translation="MTLDLLAERGAILEGHFLLTSGMHSDVYVEKARMLEDPALVARL
                     AGEIASWYERVEAVVSPAVGAIPLGFAVAAAAGARFLYTEREEGRMSLRRGFALRPGE
                     RTLVVEDVVTTGGSAREVWELVRSAGAEALGVAALVDRTAGAMGFPLRALVRVEARVF
                     EPGACPLCSSGEPLASRGSRHL"
     gene            914..1231
                     /gene="mihF"
                     /locus_tag="VF097_03585"
     CDS             914..1231
                     /gene="mihF"
                     /locus_tag="VF097_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HEX6261912.1"
                     /translation="MPLPSLTPEQRRQALEKAAEARKKRAAVKQELKKGSLTLRDVLN
                     RAGNDDILGKMRVSAVLESMPGVGKVRARKIMERLDISSSRRVRGLGSKQREALLEEF
                     SRR"
     gene            1251..1823
                     /gene="gmk"
                     /locus_tag="VF097_03590"
     CDS             1251..1823
                     /gene="gmk"
                     /locus_tag="VF097_03590"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020645227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX6261913.1"
                     /translation="MRGRLVVVSGPSGVGKGTVVRGLLAARPGLALSVSATTREPRPG
                     EVDGVHYRFLDDAAFDELIEQGAFLEWADVFGRRYGTLAEDVAEARAGGRDVLLEIDV
                     QGARTVRERVPDAILVFLQPPSEEELRRRLAARGTETGESLETRLREARREMAEADRF
                     DHVVVNDEISRAVEEVLAIIDAPIREGDPS"
     gene            1820..2071
                     /gene="rpoZ"
                     /locus_tag="VF097_03595"
     CDS             1820..2071
                     /gene="rpoZ"
                     /locus_tag="VF097_03595"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015771601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX6261914.1"
                     /translation="MIEPSIDDLLEKVDAKYSLVILAARRAREINNYYSQLGEGRGEF
                     VPPLVETGGLREKPLTIAIQEIAEDKVTAERPEEPEAEE"
     gene            2074..3267
                     /gene="coaBC"
                     /locus_tag="VF097_03600"
     CDS             2074..3267
                     /gene="coaBC"
                     /locus_tag="VF097_03600"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX6261915.1"
                     /translation="MAGPLAGRRVLLAVTGGIAAYKAAYLCRLLQERGAEVQVVMTSS
                     ATRFVGPDTFAALTGRDVHQGVFERAETVLHVRLAREADAAVVAPATANVLAKLANGI
                     ADDLVTSTLLEFRGPLVVAPAMHSGMVEAPPTRRNLEALTEAGVRIVGPVEGPLAAGD
                     EGMGRMAEPEEIAEALEKALGGGDLAGRRILVTAGPTHEPVDAVRFVGNRSSGRMGFA
                     VAEEARRRGAEVILVTGPTHLADPPAIEVRRVETAAQMREEVLRAFEGADAVVKAAAV
                     SDYRPAEPSARKLKKEEGPPPIRLEPTADILAELGGRKGERVLVGFAAETDDLEAAGR
                     RKLTEKHLDLVVVNEVGRPGTGFEAETNRAMILSARGDDEPLRDWTKRDLARAIADRL
                     VKLLT"
     gene            3287..4471
                     /gene="metK"
                     /locus_tag="VF097_03605"
     CDS             3287..4471
                     /gene="metK"
                     /locus_tag="VF097_03605"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008339192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX6261916.1"
                     /translation="MARTHLFTSESVTEGHPDKLADQISDAVLDAILTDDPGARVACE
                     TLVTSGLAIIAGEISTRTYVDLRDVVRETITEVGYTDPALGIDGEACGVITAIQEQAG
                     DIAQGLEDSWESRRGSDDELDRLGAGDQGMMIGYACTETAELMPAPIAIAHRLTKRLA
                     DVRKAGVVPYLRPDGKSQVTVEYEDDRPRRITTVVVSAQHREEVDVETLLTPDVVDHV
                     IDPVLAEVGLDAEEVVHMVNPTGRFEKGGPAADTGLTGRKIIVDSYGGLVPHGGGCFS
                     GKDPTKVDRSGAYFVRYVAKNVVAAGLADRAQIQVAYAIGTAHPVSFVLETFGTSEVD
                     PERLETTVRELFDFRPAAIIRDLDLRRPIYRETAAYGHFGRKDVPWEATEKADELRRA
                     FA"
     gene            4476..6275
                     /locus_tag="VF097_03610"
     CDS             4476..6275
                     /locus_tag="VF097_03610"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261917.1"
                     /translation="MGRAVGVAVDLPRLELDRPFTYLLPEGVGAPVGSVVSVPFHGRT
                     VHGWVLGPTDDVPARVLPVRRVLSKEPVFDQRALKLFTWMRERYVVPLSVAIDRAVPP
                     RVASEEGGAAGSPTEPRAAVPGALGRYESGGGLLTACREGSGVFVVRPLPEDEAAVCV
                     DAVTATVAGGRDAVVVIPELDPLPEVAAAVLEAAGPAGVLFAGGDRRARYRIWLEILR
                     GRYRVVVGTRPAVFSLLPRIGLLWIHREAHAVHREERTPAYHPREVALARAELEGAVC
                     ALAALSPSAHAAALVEDGEATLVRAPRATERAAAPLVETTRPADEDRSPRLAALLRST
                     PGAFLLLSRRGYGTARVCRACGEPARCAACQGPLVVREGREACAVCGEDARCAACGAA
                     DFGVERGGTERIEEWARGVTRLPVRRVDDGAASGPEQGEVVVGTAAAVKDLGSRSIGL
                     VGVLDADRARRRAGLDAPEQVLATWFEAAAWAGPRAEGGRVLVQTREPADPAVQALVR
                     WDPLHFHRRERARREEAGFAPGVPLFRVVGSPELDEALGALKPPHLLATPLGDQTVCL
                     VAIRPDALTAFRERILDLVGEHVVARVEAEPRL"
     gene            6289..6813
                     /gene="def"
                     /locus_tag="VF097_03615"
     CDS             6289..6813
                     /gene="def"
                     /locus_tag="VF097_03615"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014351715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX6261918.1"
                     /translation="MAILPIRTLGDPVLRQPASPVEEFDDPLRALADDMIETMHEAPG
                     VGLAAPQVGRAVRLIVFDVGEGPIALANPTLSGHDGEQIGEEGCLSIPGLYFEVRRAM
                     KVTADGFDLHGEPVRIEGEELLARVLQHEVDHIDGILFIDRLDEEERKRAMAELRRQE
                     LGLAPTSLDPSRAL"
     gene            6814..7731
                     /gene="fmt"
                     /locus_tag="VF097_03620"
     CDS             6814..7731
                     /gene="fmt"
                     /locus_tag="VF097_03620"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX6261919.1"
                     /translation="MRRRVVFLGNDPWSVPSLEALASSEHKVALVLTREPRPAGRGGR
                     LRPTAVAEAARGLGLPLREVATVKEGEGREALEEAVPDVLVVVAYGEILPGDVLAVPR
                     LAPVNVHFSLLPELRGADPVRRAILEGLETTGVTTMRMDEGMDTGPILLQAEETIRSE
                     DDAGSLGERLAALGGRLLVETLDRLPGLAERPQDDAGATIARKIPPEQEWIDWSQPVE
                     AIVRRVRALSPEPGARTRMRGKVLKVYRARSVEGAGEPGTIVDPGEMTVAAGGGAVAL
                     DEILLEGKRRMSGAELARGHRPEPGERLG"
     gene            7793..9058
                     /locus_tag="VF097_03625"
     CDS             7793..9058
                     /locus_tag="VF097_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX6261920.1"
                     /translation="MAIEAVRRVADEGAYSNRLIPSLIGRSDLGPEDRRLASELAYGT
                     LRRLLPIGAALAPLLDRPLRRAPSGARAALRVGAYQLLHTRIPPHAAVGETVAVAPPR
                     ARGFVNAVLRRLADEGWRPPEGDDDGSISLRTGVAPWVVAQLRALVGDEAEPAAAGIA
                     SPAGLTLRANPCAGPLEDLPGILAAAGHEVEVGGIHAGSFRLPGGDPRTLPGWEDGLF
                     TVQDEASAWVVDVLDPRPGERVLDCTAGPGGKATDIACRAGSVVASDLSERRAALVAS
                     TAARLRVDAHPLVMDAARPAVRPGFDRVLVDAPCSGLGSARRRPELLWRPKEDDLAAL
                     RRLQVDLVSSAGGLLRSGGVLVYSVCTFPRPETDEVVAEVLARVPDLRPDPFTTPDGE
                     RRASARLWPHRHGTDAMFAARFVLGGSRP"
     gene            9071..9739
                     /locus_tag="VF097_03630"
     CDS             9071..9739
                     /locus_tag="VF097_03630"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX6261921.1"
                     /translation="MSQIRVAASILAADFARLGEQVKAVEPYAEIIHVDSMDAHFVPP
                     LTIGPVVIESIRPLTERLLHAHMMVERPERLLEAYGAAGTDIATFHLEAASDPDAVLD
                     EAETLGLRAGIGVNPETEIEEVHPFLERLDNVIVMTLERTGYAGQPFQREHLRKVEVV
                     RKEIDRLGLAVDVVVDGGIDAETGRLCVDAGATVLAAASSIFRAEDPAAAARGLAEAT
                     RADG"
     gene            9736..10707
                     /locus_tag="VF097_03635"
     CDS             9736..10707
                     /locus_tag="VF097_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013732795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6261922.1"
                     /translation="MSDEGAASRPEPGNTRVLVVDDDPDIARFVEVNLRGDGYAVEIA
                     SNGDQGLEKALANPPDLALVDVMMPGMDGFQLVERLRTDPRTAATSIIMLTAKSLTAD
                     KVLGLTTGADDYIIKPFDPVELLARVKGTLRRAEEMRASSPLTGLPGNVRIQDEIARV
                     VASGGPFALVYADLDNFKAYNDYYGFLRGDAAIKTAARIFQEETRMVAGESAFVGHMG
                     GDDFVLLVPPEKVEGLCHAIIERFDGAMTELYDDEDRERGYIEIRSRRGDLQRFPLVS
                     LSLGVATTERRSFTHREEAVAIATEMKNYAKRSDGSSWAIDRRSAGR"
BASE COUNT         1420 a         3838 c         4154 g         1431 t
ORIGIN      
        1 tcgccttcga ggcgagcgag gccggcgccc gcggggtcgt ctgctccggc gatcacgtcc
       61 gggaggtccg ggacgcgctg ggcgaggatc ctctgatcgt ggtgccgggg atccgtccgg
      121 gagggatggg ggcgaacgac cacgccagga ccctgacacc ccgggaggcc gtggagcgcg
      181 gcgccgacct cctcgtcgtg ggccgcccca tcacccaggc cgccgacccc gcacaggccg
      241 cacgggccat cgcgcgggag ctcgagggat gacgctcgac ctgcttgcgg agcggggagc
      301 gatcctcgag gggcacttcc tcctcacctc ggggatgcac tccgacgtct acgtggagaa
      361 ggcgcggatg ctcgaggacc ccgcgctcgt ggcacggctc gccggcgaga tcgcttcctg
      421 gtacgagcgc gtcgaagcag tcgtgtcccc ggcggtcggc gcgatccccc tcgggttcgc
      481 ggtggccgcg gccgccggag cccgcttcct ctacacggag cgagaggagg ggcggatgtc
      541 gctgcgacgc ggcttcgccc ttcgccccgg ggagcggact ctcgtcgtgg aggacgtcgt
      601 gacgaccggc ggctccgccc gcgaggtgtg ggagctcgtc cgctcggcgg gcgccgaggc
      661 cctcggggtc gccgcgctcg tggaccggac cgcgggagcg atgggcttcc ccctcagggc
      721 cctggtccgt gtggaggccc gggtgtttga gcccggggcg tgtccgctct gctcgtcggg
      781 ggagcccctc gccagccgcg gctcgcgcca cctctgaccg tccgccgctt gtgacctgct
      841 cgttccgcat tgccgcccgg aaaacggcgt gggtataatc ccggcgcttc cgaagccacc
      901 tgaaggagga tgcatgcccc tgccatcgct cacccccgaa cagcgacgtc aggccctcga
      961 gaaagccgcg gaggcccgca agaagcgagc cgcggtcaag caggagctga agaagggctc
     1021 cctcacgctc cgcgacgtcc tgaaccgggc agggaacgac gacatcctgg ggaagatgcg
     1081 ggtctcggcc gtcctcgagt cgatgcccgg cgtgggcaag gtccgggccc gcaagatcat
     1141 ggagcggctg gacatctcct cgagccgccg cgtgcgcggg ctcgggtcga agcagcggga
     1201 ggccctcctc gaggagttct cgcggcgctg aggcgccggg gcggccgcag gtgcgaggcc
     1261 gcctcgtcgt cgtctccggg ccctcgggcg tggggaaggg gaccgtggtc cgcgggctgc
     1321 tcgcggcccg gcccggcctg gcgctctcgg tctcggccac gacacgcgag ccgcgtccag
     1381 gcgaggtgga cggcgtccac taccgcttcc tggacgacgc cgcgttcgac gagctgatcg
     1441 agcagggcgc cttcctggag tgggcggacg tgttcggccg ccgctacgga accctggccg
     1501 aggacgtggc ggaggctcgg gccggcggca gggacgtgct cctcgagatc gacgtccagg
     1561 gcgcgcgaac cgtgcgggag cgtgtgccgg atgcgatcct cgtgttcctc cagccaccgt
     1621 cggaggagga gcttcgccgg cgcctggccg cgcggggcac cgagacgggg gagtcgctgg
     1681 agacgaggct ccgcgaggcc cgccgggaga tggccgaggc cgaccgattc gaccacgtgg
     1741 tcgtgaacga cgagatctcc cgagccgtgg aggaggttct tgctatcatc gacgcaccga
     1801 tccgcgaagg agacccctct tgatcgagcc cagcatcgac gacctgctcg agaaggtcga
     1861 cgctaagtac tcgctcgtga tcctggctgc gcgccgggcc cgcgagatca acaactacta
     1921 cagccagctc ggggaggggc gcggcgagtt cgttccgccc ctcgtcgaga ccggcgggct
     1981 ccgggagaag ccgctgacga tcgcgatcca ggagatcgcg gaggacaaag tcaccgccga
     2041 gcggccggag gagcccgagg ccgaggagta gacgtggccg gccccctggc cgggcggcgc
     2101 gtcctcctgg cggtcacggg gggcatcgcc gcgtacaagg ccgcctatct ctgcaggctc
     2161 ctgcaggagc ggggggccga ggtccaggtc gtgatgacgt cctcggccac gcgcttcgtc
     2221 ggccccgaca ccttcgccgc cctcaccggc cgcgacgtcc accagggcgt cttcgagcgg
     2281 gccgaaaccg tcctccacgt ccggctggcc cgcgaggccg acgccgcggt cgtggccccc
     2341 gccacggcca acgtcctggc gaagctggcg aacggcatcg ccgacgacct cgtgacctcg
     2401 accctcctgg agttccgcgg tccgctcgtg gtggccccgg ccatgcactc ggggatggtc
     2461 gaggcccctc cgacgcgacg caacctcgag gcgctgacgg aagccggcgt gaggatcgtg
     2521 ggccccgtgg aggggccgct ggccgcgggg gacgagggca tggggcggat ggccgagccc
     2581 gaggagatcg ccgaggcgct ggagaaggcg ctcgggggcg gggacctggc ggggaggcgg
     2641 atcctcgtga cggccggccc cacccacgag ccggtggacg ccgtgcgctt cgtcgggaac
     2701 cgatcgagcg gccggatggg cttcgcggtc gccgaggagg cgcggcgccg gggcgccgag
     2761 gtgatcctcg tcacgggccc cacacacctc gccgaccccc ctgcgatcga ggtgcggagg
     2821 gtggagacgg cggcccagat gcgcgaggag gtcctccgcg ccttcgaggg cgccgatgcg
     2881 gtggtgaagg cggcggccgt gagcgactac cgcccggccg agccgtccgc ccgcaagctg
     2941 aagaaggagg agggtccgcc cccgatccgg ctggagccta ccgccgacat cctcgccgag
     3001 ctgggggggc ggaagggcga gcgcgtgctg gtcggcttcg ccgccgagac cgacgacctc
     3061 gaggccgccg gccgccggaa gctgacggag aagcacctcg acctcgtcgt cgtgaacgag
     3121 gtgggtcgtc cgggcaccgg cttcgaggcc gagacgaacc gcgcgatgat cctctcggct
     3181 cggggcgacg acgagccgct ccgcgactgg acgaagcggg acctggcccg ggccatcgcc
     3241 gaccgcctgg tgaagctcct gacctgaggc ctgtagaatc ccgggcatgg cgcggacaca
     3301 cctcttcacc tcggagtcgg tgaccgaagg gcaccccgac aagctcgccg accagatctc
     3361 cgacgccgtc ctcgacgcga tcctcaccga cgacccgggc gcccgcgtgg cctgcgagac
     3421 gctcgtgacc tcgggcctgg cgatcatcgc gggcgagatc tcgacccgca cctacgtgga
     3481 cctccgcgac gtcgtccgcg agaccatcac tgaggtcggg tacaccgacc cggccctggg
     3541 gatcgacggc gaggcctgcg gcgtcatcac cgcgatccag gagcaggcgg gggacatcgc
     3601 ccagggcctg gaggattcgt gggagtcgcg ccgcggctcg gacgacgagc tcgaccggct
     3661 cggggccggc gaccagggca tgatgatcgg gtacgcctgc accgagacgg ccgagctgat
     3721 gccggctccg atcgccatcg cccaccgcct gacgaagcgg ctggccgacg tccgcaaggc
     3781 cggcgtcgtg ccgtacctcc gccccgacgg caagagccag gtcacggtgg agtacgagga
     3841 cgaccgcccc cggcggatca ccacggtggt ggtctcggcc cagcaccggg aggaggtcga
     3901 cgtcgagacg ctcctcacgc ccgacgtcgt ggaccacgtg atcgaccccg tgctggccga
     3961 ggtgggcctc gacgcggagg aggtcgtcca catggtgaac cccaccggcc ggttcgagaa
     4021 aggggggccg gccgccgaca cgggcctcac cggccggaag atcatcgtgg actcctacgg
     4081 gggactcgtt ccccacggcg gcggctgctt ctcggggaag gaccccacga aggtggaccg
     4141 gtccggcgcc tacttcgtgc gctacgtggc gaagaacgtc gtggccgcgg ggctcgcgga
     4201 ccgggcccag atccaggtcg cctacgccat cggtaccgcg caccccgtct cgttcgtgct
     4261 ggagaccttc gggacgtcgg aggtggaccc ggagaggctc gagaccaccg tgcgcgagct
     4321 gttcgacttc cggccggcgg cgatcatccg ggacctggac ctgcggcgcc cgatctaccg
     4381 ggagaccgcc gcctacggcc acttcggtcg caaggacgtc ccctgggagg ccaccgagaa
     4441 ggccgacgag ctccgccgcg cgttcgcctg aggccgtggg gcgggccgtc ggggtggcgg
     4501 tggacctccc ccgcctggag ctcgaccgcc cgttcaccta cctcctcccc gagggcgtgg
     4561 gggccccggt gggctcggtc gtcagcgtcc ccttccacgg gcgcaccgtc catggatggg
     4621 tgctgggccc caccgacgac gtcccggcgc gggtgctgcc cgtgcgccgg gtcctgtcga
     4681 aggagcccgt cttcgaccag cgggcgctga agctcttcac ctggatgcgg gagcgctacg
     4741 tggtgccgct gtcggtggcg atcgaccgcg cggtgccgcc gcgggtggcg tccgaggagg
     4801 gaggggcggc ggggtccccc acggagcccc gggccgccgt cccgggagcc ctgggccggt
     4861 acgagagcgg aggagggctg ctgacggcgt gccgcgaggg gagcggggtg ttcgtcgtcc
     4921 gccccctccc cgaggacgag gccgccgtct gtgtggacgc cgtgacggcc acggtggccg
     4981 gcgggcgaga cgccgtggtc gtgatccccg agctcgaccc gctgccggag gtcgcggcgg
     5041 cggtgctcga ggcggccggg ccggccggcg tcctcttcgc gggaggggat cggcgggctc
     5101 ggtaccggat ctggctggag atcctgcggg ggcgctatcg ggtcgtggtg gggacgaggc
     5161 cggccgtttt ctcgctcctc ccgaggatcg gcctcctctg gatccatcgc gaggcgcacg
     5221 ccgtccaccg cgaggagcgg acgcccgcgt accacccgag ggaggtggcg ctggcgcgag
     5281 cggagctcga gggggcggtg tgcgccctcg ccgccctctc tccgagcgcg catgcggcgg
     5341 ccctggtcga ggacggcgag gcgacgttgg tccgggcccc gcgggcgacc gagcgcgccg
     5401 cagctcccct cgtggagacg acgaggccgg ccgacgagga tcgctccccg aggctggcgg
     5461 cgttgctgcg gtcgacgccc ggcgcgttcc tcctcctttc gcggcggggc tacgggacgg
     5521 cacgcgtctg ccgggcctgc ggcgagcctg cccgctgcgc cgcgtgccaa ggaccgctcg
     5581 tcgtgcggga aggacgcgag gcctgcgccg tctgcgggga ggacgcccgc tgcgccgcct
     5641 gcggggccgc cgacttcggc gtggagcggg gggggaccga gcggatcgag gagtgggccc
     5701 gcggcgtgac acgcctcccg gtccgccggg tggacgatgg cgccgcgtcg gggcccgagc
     5761 agggggaggt cgtcgtgggc accgcagcgg cggtgaagga cctggggtcg aggtcgatcg
     5821 ggctcgtggg cgtcctggac gcggatcgcg ccaggcgtcg ggccgggctg gatgcgcccg
     5881 agcaggtcct cgccacctgg ttcgaggccg cggcctgggc tggccctcgc gccgaggggg
     5941 ggagggtcct cgtccagacg cgggagccgg ccgaccccgc ggtgcaggcg cttgtgcggt
     6001 gggatccgct ccacttccac cgccgcgagc gggcccgccg ggaggaggcg gggttcgcgc
     6061 ccggcgtccc cctcttccgg gtcgtgggct cgccggagct ggacgaggcg ctcggggcgc
     6121 tcaagccccc gcacctgctc gcgacgccgc tgggggacca gacggtatgc ttggtggcga
     6181 tccgccccga cgccctcacg gcgttccggg agcgcatcct cgacctcgtc ggcgagcacg
     6241 tcgtcgcgcg ggtcgaggcc gagccgcgac tgtgacgagg agtgcgagat ggcgatcctt
     6301 ccgatccgga cgctgggcga cccggtgctg cgccagccgg cctccccggt ggaggagttc
     6361 gacgaccccc tccgggcgct ggccgacgac atgatcgaga cgatgcacga ggctcccggc
     6421 gtgggcctgg ccgccccgca ggtggggcgg gcggtccggc tgatcgtgtt cgacgtcggg
     6481 gagggtccga tcgcgctggc caaccccacg ctctcgggcc acgatgggga gcagatcggc
     6541 gaggagggct gcctctcgat cccgggcctc tacttcgagg ttcgccgggc gatgaaggtg
     6601 acggccgacg gcttcgacct ccacggggag ccggtgcgca tcgagggcga ggagctgctg
     6661 gcccgcgtcc tccagcacga ggtggatcac atcgacggca tcctgttcat cgaccgtctg
     6721 gacgaggagg agcggaagcg cgcgatggcc gagctccgcc ggcaggagct cgggctggcg
     6781 cccacctcgc tagacccctc gcgcgcgctc tgagtgagac ggcgcgtcgt cttcctcggg
     6841 aacgacccct ggtcggtccc ctcgctggag gccctggcct cttcggagca caaggtcgcc
     6901 ctcgtcctga cccgggagcc ccggcccgcc gggcgcgggg gccggctccg gccaacggcg
     6961 gtggccgagg cagcccgagg gctggggctg ccgctccgtg aggtggccac ggtcaaggaa
     7021 ggcgagggcc gggaggctct ggaggaggcc gtgcccgacg tcctcgtggt cgtggcctac
     7081 ggcgagatcc tgccgggcga cgtgctcgcg gtcccgaggc tcgcccccgt caacgtgcac
     7141 ttctcgcttc tccccgagct ccggggcgca gacccggttc ggagagcgat cctggagggc
     7201 ctcgagacga ccggcgtgac caccatgcgg atggacgagg ggatggacac ggggcccatc
     7261 ctcctgcagg cggaggagac gatccgttcg gaggacgacg cggggtcgct gggggagcgt
     7321 ctggctgcgc tgggcggacg tctgctcgtt gagaccctgg accggctgcc gggcctcgcg
     7381 gagcgcccgc aggacgacgc gggcgccacg atcgcccgga agatcccccc ggagcaggag
     7441 tggatcgact ggtcccagcc cgtcgaggcg atcgtgcgcc gggtgcgggc gctctcgccg
     7501 gagccgggag ctcggacgcg gatgcggggg aaggtcctga aggtgtatcg ggcgcggtcg
     7561 gtggagggcg ccggcgagcc agggacgatc gtggatcccg gggagatgac ggtggccgcc
     7621 ggcggggggg ccgtggccct ggacgagatc ctgctcgagg ggaagcggcg gatgagcgga
     7681 gcggagctgg cccgtggcca caggccggaa ccgggcgagc ggctcgggta gggggcggcg
     7741 cggcgcacgg cctcggggcc gggccgcgcc cccgctgcgc accccgcggg cggtggcgat
     7801 cgaggccgtg cgccgcgtcg ccgacgaggg cgcgtacagc aaccggctca tcccctcgct
     7861 gatcgggcgc tcggacctgg gccccgagga ccggaggctg gcctcggagc tcgcctacgg
     7921 caccctgcgg cgtctcctgc ccatcggtgc ggcgctcgcg ccactcctgg accgacccct
     7981 tcgtcgcgcg ccgagcggcg ctcgcgccgc cctccgcgtc ggcgcctacc agctcctgca
     8041 cacccgcatc cccccgcacg ccgccgtggg cgagacggtc gctgtggcgc cgccccgggc
     8101 tcgcgggttc gtgaacgccg tcctccgacg cctggccgac gagggctggc gcccgccaga
     8161 gggggatgac gacggctcga tctccctgcg caccggggtc gcgccctggg tcgtggccca
     8221 gctccgcgcc ctcgtgggcg acgaagccga gccggcggcg gccgggatcg cctccccggc
     8281 tggcctcacc ctgcgggcca acccgtgcgc ggggccgctc gaggatctcc ccggcatcct
     8341 ggccgccgcc ggtcacgagg tcgaggtggg gggcatccac gccgggtcgt tccgcctccc
     8401 cggcggcgac cccaggacgc tccccgggtg ggaggacggc ctcttcaccg tccaggacga
     8461 ggcctcggcg tgggtggtgg acgtgctgga tccccggccg ggggagcggg tgctggactg
     8521 cacggccggc ccggggggga aggccaccga catcgcgtgc cgggcggggt ccgtcgtcgc
     8581 ctcggacctc tcggagcgtc gcgcggccct ggtagcgtcc acggccgccc ggctgcgggt
     8641 cgacgcccac ccgctcgtca tggacgcggc ccgacccgcc gtccgccccg gcttcgaccg
     8701 ggtgctggtg gatgccccgt gctcgggcct gggctcggcc cgccgccgcc ccgagctcct
     8761 ctggcggccc aaggaggatg acctcgccgc gctgcggcgc ctccaggtcg acctcgtctc
     8821 gagcgcgggc ggcctcctgc gatcgggcgg cgtgctcgtg tactcggtct gcaccttccc
     8881 ccgcccggag acggacgagg tcgtggcgga ggtcttggcc cgggtcccgg atctgcgtcc
     8941 cgacccgttc acgaccccgg atggtgagcg ccgggcctcg gcccgtctct ggccccaccg
     9001 ccacggcacc gacgcgatgt tcgcggcccg cttcgtcctg ggtgggtccc ggccgtagaa
     9061 tcggggcgcc atgtcgcaga tcagggtggc cgcctcgatc ctcgccgcgg acttcgcccg
     9121 cctcggggag caggtcaagg cggtcgagcc ctacgccgag atcatccatg tggactccat
     9181 ggacgcgcac ttcgtcccgc ccctcacgat cgggcccgtc gtgatcgagt cgatccggcc
     9241 gctgaccgag cggctgctcc acgcgcacat gatggtggag cgtcccgagc ggctcctcga
     9301 ggcctacgga gcggcgggga ccgacatcgc caccttccac ctcgaggcgg cctcggaccc
     9361 cgacgcggtg ctcgacgagg ctgagaccct cggcctccgg gcgggcatcg gggtgaaccc
     9421 cgaaaccgag atcgaggagg tccacccctt cctggagcgg ctcgacaacg tgatcgtgat
     9481 gacgctggag cgcaccgggt acgccggtca gcccttccag cgggagcacc ttcggaaggt
     9541 ggaggtggtg cggaaggaga tcgaccgact cgggctcgcg gtggatgtgg tggtcgacgg
     9601 cggcatcgac gcggagaccg ggcgtctgtg cgtggacgcg ggcgccacgg tcctggcggc
     9661 ggcctcctcc atcttccggg ccgaggaccc ggcggcggcc gcgcgcggcc tggccgaggc
     9721 cacgagggcc gacggatgag cgacgagggc gcggcctccc ggcccgagcc cggcaacacc
     9781 agggtcctcg tggtggacga tgatcccgac atcgcccgct tcgtcgaggt caacctccgg
     9841 ggcgacggct acgccgtgga gatcgcgtcg aacggcgacc aggggctgga gaaggctctg
     9901 gccaacccgc ccgacctggc cctggtggac gtgatgatgc cggggatgga cggcttccag
     9961 ctcgtggagc gcctccggac cgatccacgc acggccgcca cgagcatcat catgctgacg
    10021 gcgaagtcgc tcaccgccga caaggtgctg gggctgacga ccggggccga cgactacatc
    10081 atcaagccgt tcgacccggt cgagcttctg gcgcgggtca agggcaccct gcggcgggcc
    10141 gaggagatgc gggcttcctc gcccctcacc ggcctcccgg gcaacgtcag gatccaagac
    10201 gagatcgccc gggtcgtcgc ctcgggtggg ccgttcgccc tcgtctacgc cgacctcgac
    10261 aacttcaagg cctacaacga ctactacggg ttcctgcggg gcgatgccgc gatcaagacg
    10321 gcggcgagga tcttccagga agagacgagg atggtggccg gcgagtcggc cttcgtgggc
    10381 cacatgggcg gagacgactt cgtgctgctg gtcccccccg agaaggtcga gggcctctgc
    10441 cacgccatca tcgagcggtt cgacggcgcg atgaccgagc tctacgacga cgaggatcgg
    10501 gagcgcggct acatcgagat ccggagccgg cgcggggacc tgcagcggtt cccgctcgtc
    10561 tcgctctccc tcggagtggc caccaccgag cggcgctcgt tcacccaccg cgaggaggcg
    10621 gtggccatcg ccaccgagat gaagaactac gcgaagcggt ccgacggctc ctcctgggcc
    10681 atcgaccgaa ggtccgccgg ccgctgagcg ccgttctata ctggtcgggc aaccgagcct
    10741 tcggggcggg gtggaactcc ccaccggtgg tgacagtcca cgaccccccg cgagccacgc
    10801 gggggtggac ccggcggaat cccgggaccg acggtgacag tcc
//
LOCUS       DASUTZ010000081         4877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_540354, whole genome shotgun sequence.
ACCESSION   DASUTZ010000081 DASUTZ010000000
VERSION     DASUTZ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540354"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="VF097_03640"
     CDS             <1..852
                     /locus_tag="VF097_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007581480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0568 family radical SAM protein"
                     /protein_id="HEX6261923.1"
                     /translation="ETEDGIPYWQIALLHLDSLASTVVQTCVHWGTPDQCTFCGIEVS
                     LEAGATIPVKKPEQLAEVARAAKDLDGAVDVTLTTGTTRGPDRGARYLGKSAAAIRDA
                     AGLPVQAQFEPPDDYSVFEEIKEQGVTAVGMHVETFDPQVLERVGPAKARTGVEGYFR
                     AWEAAVEVFGRGQVSTYVILGMGEDPRLTVEGCRRAVDVGIYPFVVPLRPVAGSLMED
                     ELPPAPDYVESLYRQITPYVVANGLGSWDVSAGCARCRACSGMSAFEAVEGRDAGRRS
                     LPILEVS"
     gene            890..1324
                     /locus_tag="VF097_03645"
     CDS             890..1324
                     /locus_tag="VF097_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0567/Sll0786 family nitrogen starvation
                     N-acetyltransferase"
                     /protein_id="HEX6261924.1"
                     /translation="MASSPDEEAIHHEIRRRVFVEEQGFFQGTDRDEHDDDRRTLKVL
                     ALWGPVAGGAVRLYPLDEEGLWKGDRLAVLDPFRRRRLGGPLVRFAVKTAGERGGRRM
                     LAHVQVPNVPFFQRLGWDAIGEPEEFVGHLHQVMAIDLGPAG"
     gene            1445..1954
                     /locus_tag="VF097_03650"
     CDS             1445..1954
                     /locus_tag="VF097_03650"
                     /inference="COORDINATES: protein motif:HMM:NF014360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmiS/UreI family transporter"
                     /protein_id="HEX6261925.1"
                     /translation="MPPIAIVFFLTGIAVWANALYFLGVAAERQEGGSDPLRAVGWIT
                     LLAGISNFWQVSQIGTQDNLLGGLVVFYAAFFTFLGITEILALDLRVLGHVSIAVALL
                     PLVYWDFLSGSWMLQSILVVWAIVFLAIAAVTYGRLSGRVLGVLLLATAIYTFWIPAS
                     ILALGNTIP"
     gene            complement(1956..2897)
                     /locus_tag="VF097_03655"
     CDS             complement(1956..2897)
                     /locus_tag="VF097_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEX6261926.1"
                     /translation="MTTLDEIVRRYREHPGLGAKRPLRIVREALGPSDAVTGPGDDAA
                     AIKHGDGHLLVAGEAMWPPFAEADPFGAGAAAVVANVNDVAAMGGRPLAIVDTIVGPE
                     EDARRALEGMGWAADLYGVPVVGGHLTVAKGPPSISAFVAGRARTLLSATRVAPGQVL
                     LVACCREGRMREDFPFFSSLRERGERLRDDVAVLADVAEGDLAEAAKDVSMAGFLGSL
                     AMLLEPTRAGATVDLDAMPRPEGVANEDWAGAFPTYGFWLTARPEAAEDCRDAFLSRG
                     LASELVGELDDTGVLRVRFAGEVGDLADLARDPPTGL"
     gene            2966..3895
                     /gene="mdh"
                     /locus_tag="VF097_03660"
     CDS             2966..3895
                     /gene="mdh"
                     /locus_tag="VF097_03660"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX6261927.1"
                     /translation="MKVTVVGSGFVGATTAMRVAQQGLADEVVMIDIVEGLPQGLALD
                     MNQSSPIEGFEPVVRGTNDYADTAGSDLVIITAGLPRQPGMSRMDLLEKNAGIMRDVA
                     GQVHDRSPDAVVIVVSNPLDEMTFLAAQVTGFPKERVMGMAGTLDSARLRTFIAEELG
                     VSPSSVEAMTLGSHGESMVPLPGRATVDGKPLRDLVDDETLERLYARTRDAGAEIVGH
                     LKKGSAYYAPSAAVAQMARAITGDTKEVLPVCAWTAGEYGIDGVYVGVPARLGRGGVE
                     EIVELDLEEQEMAALREAAEEIRAKCAELSEAG"
     gene            3895..4056
                     /locus_tag="VF097_03665"
     CDS             3895..4056
                     /locus_tag="VF097_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014259546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261928.1"
                     /translation="MGEIGDRTGDFRDNWRRYEGPLWRKLRIAARNRAIAASMVRGGC
                     CGHHGEPGC"
     gene            complement(4113..4331)
                     /locus_tag="VF097_03670"
     CDS             complement(4113..4331)
                     /locus_tag="VF097_03670"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HEX6261929.1"
                     /translation="MAGCARTGALGKLIKRDPKLKALRSNGGPTRTHALKQGSRAIGR
                     ATKKLAPKRDQRGILRDGKPDLGAYERT"
     gene            complement(4647..>4877)
                     /locus_tag="VF097_03675"
     CDS             complement(4647..>4877)
                     /locus_tag="VF097_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011974124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX6261930.1"
                     /translation="PVHGSAPDIAGKGVANPIAMILSAALMLRHLSEDHAASRVEAAV
                     ASVIAERRILTPDMGGAASTREVAETIAARVG"
BASE COUNT          667 a         1703 c         1808 g          699 t
ORIGIN      
        1 gagaccgagg acggcatccc gtactggcag atcgcgctgc tgcacctcga ctcgctggcc
       61 agcaccgtgg tgcagacgtg cgtccactgg ggcacgcccg accagtgcac gttctgcggg
      121 atcgaggtct cgctcgaagc cggggccacg atcccggtga agaagccgga gcagctggcc
      181 gaggtggcga gggcggcgaa ggacctcgac ggcgcggtgg acgtgacctt gacgaccggg
      241 accacgcgcg gccccgaccg gggggcccgc tacctcggga agagcgccgc cgcgatccgg
      301 gacgccgcgg gcctgcccgt ccaggcgcag ttcgagcccc cggacgacta ctcggtgttc
      361 gaggagataa aggagcaggg cgtcaccgcc gtcggcatgc acgtcgagac cttcgacccg
      421 caggtgctgg agcgggtggg gccggccaag gcccggaccg gcgtggaggg gtacttccgg
      481 gcctgggagg ccgcggtcga ggtgttcggc aggggccagg tgtcgaccta cgtgatcctg
      541 ggcatgggcg aggatcccag gctcacggtg gagggatgtc gccgggccgt ggacgtgggc
      601 atctacccgt tcgtcgtgcc gctgcggccg gtggccggga gcctcatgga ggacgagctg
      661 ccaccggccc ccgactacgt cgagtcgctg taccggcaga tcacgcccta cgtcgtggcg
      721 aacgggctcg gttcgtggga cgtgagcgcg gggtgcgcgc gctgccgcgc gtgctcgggg
      781 atgagcgcct tcgaggccgt ggagggcagg gacgctggac gccgcagtct ccccattctc
      841 gaggtcagct gaggccgcgc tccgccggcg gctgcccgtc acctgtcgcg tggcgagctc
      901 gcccgacgag gaggcgatcc accacgagat ccgccgccgg gtcttcgtgg aggagcaggg
      961 cttcttccag ggcaccgatc gcgacgagca cgacgacgat cgtcggacgc tgaaggtgct
     1021 cgcgctgtgg gggccggtgg cggggggcgc ggtgcgcctc tatccgctgg acgaggaagg
     1081 cctctggaag ggcgaccggc tcgccgtgct ggatcccttc cgcaggcgcc gcctcggggg
     1141 ccccctcgta cggttcgcgg tgaagacggc gggggagcgg ggcggccgcc ggatgctggc
     1201 ccacgtccag gtccccaacg tgccgttctt ccagcgcctg gggtgggacg cgatcggcga
     1261 gcccgaggag ttcgtcggcc acctccacca ggtgatggcg atcgacctcg gtccggccgg
     1321 ctgacatccc gccgttcccg ccggaacccc cgaacccccc ttcctgctcg ctctgccatt
     1381 taccatggac gacggcccgt ctactatccg gcgaaggaac cgaatcgggg aagggagggt
     1441 ggccatgcca ccgatcgcga tcgtgttctt cctcacgggc atcgccgtgt gggcgaacgc
     1501 cctgtacttc ctgggcgtcg ccgccgagcg gcaggagggc gggagcgacc cgctccgggc
     1561 cgtcgggtgg atcacgctgc tggcggggat ctcgaacttc tggcaagtca gccagatcgg
     1621 gacgcaggac aacctgctgg gcggcctcgt ggtgttctac gccgcgttct tcacgttcct
     1681 ggggatcacc gagatcctcg ccctggacct ccgggtcctg gggcacgtgt cgatcgcggt
     1741 ggcgctcctg ccgctggtct actgggactt cctgtcggga agctggatgc tccagtcgat
     1801 cctcgtcgtc tgggcgatcg tgttcctggc gatcgccgcc gtgacgtacg ggaggctgag
     1861 cgggcgcgtg ctgggggtgc tgctgctggc cacggcgatc tacacgttct ggatcccggc
     1921 gtcgatcctg gcgctgggga acacgatccc gtaggtcaga ggccggtcgg ggggtcgcgg
     1981 gcgaggtcgg cgagatcgcc gacctcgccc gcaaatcgga cccgcagcac gccggtgtcg
     2041 tccagctccc cgacgagctc ggaggcgagg ccgcgcgaga ggaaggcgtc cctgcagtcc
     2101 tcggccgcct cgggcctcgc cgtcagccag aacccgtagg tcgggaaggc gccggcccag
     2161 tcctcgttcg ccacgccttc gggccggggc atggcgtcga gatccacggt ggccccggcg
     2221 cgcgtgggct cgagcagcat cgccagcgag ccgaggaacc ccgccatgct cacgtctttg
     2281 gccgcttcgg ccaggtctcc ctccgcgacg tccgccagga ccgcgacgtc atcgcgtagc
     2341 cgctcgcccc gctcccgcag ggacgagaag aaggggaagt cctcgcgcat gcgcccctcg
     2401 cggcagcatg cgaccagcag cacctgcccg ggagcgacgc gcgtcgcgga cagcaacgtg
     2461 cgtgctcgcc ccgcgacgaa ggccgagatc gagggcggtc ccttcgccac cgtcaggtgc
     2521 ccccccacga ccggcacgcc gtagaggtcg gccgcccagc ccatcccctc gagcgcccgc
     2581 cgcgcgtcct cctccggtcc cacgatcgtg tccacgatcg cgagcggccg gccgcccatc
     2641 gccgccacgt cgttcacgtt ggccacgacc gccgccgccc cggccccgaa cggatccgcc
     2701 tcggcgaagg gcggccacat cgcctcgccg gccacgagga gatggccgtc cccgtgcttg
     2761 atcgccgcgg cgtcgtcccc cggccccgtg accgcgtcgg acggccccag cgcctcgcgc
     2821 acgatccgca gaggccgctt ggcgccaagc ccgggatgct cgcggtagcg gcggacgatc
     2881 tcgtccaggg tggtcacccg ggcatcatag aggccgcgcg ggtcggtagg atgagcccgt
     2941 cccggcgatc gaggaggcgt tcgcgatgaa ggtgaccgtg gtggggagcg gcttcgtggg
     3001 cgccacgacc gcgatgcgcg tggcccagca ggggctggcc gacgaggtcg tcatgatcga
     3061 catcgtggag gggctcccgc aggggctcgc cctggatatg aaccagtcct cgccgatcga
     3121 ggggttcgag cccgtcgtgc gcgggacgaa cgactacgcc gacacggccg gctcagacct
     3181 cgtgatcatc accgcgggcc tccctcgcca gccggggatg agccggatgg atctcctgga
     3241 gaagaacgcc gggatcatgc gcgacgtcgc gggccaggtc cacgatcgct cgcccgacgc
     3301 cgtggtgatc gtcgtctcca acccgctcga cgagatgacg ttcctcgccg cgcaggtcac
     3361 cgggttcccc aaggagcgcg tcatgggcat ggcggggacg ctcgactccg ctcggctccg
     3421 cacgttcatt gccgaggagc tgggggtctc gccctcctcg gtggaggcca tgacgctggg
     3481 ctcgcacggc gagtcgatgg tgcccctccc cggccgggcc acggtggacg gcaagccgct
     3541 gcgcgacctc gtggacgacg agaccctgga gcgcctctac gccaggacgc gcgacgcggg
     3601 cgctgagatc gtgggccacc tcaagaaggg gagcgcctac tacgcgccgt ccgccgccgt
     3661 ggcccagatg gcgcgggcca tcaccgggga cacgaaggag gtcctgcccg tgtgtgcctg
     3721 gaccgccggg gagtacggga tcgacggcgt gtacgtgggc gtccccgccc gcctgggacg
     3781 cggcggggtg gaggagatcg tggagctgga cctcgaggag caggagatgg ccgcgctgcg
     3841 cgaggcggcc gaggagatcc gggccaagtg cgcggagctg agcgaggcgg gctgatgggc
     3901 gagatcggcg atcgcaccgg cgacttccgg gacaactggc ggcgctacga gggccccctc
     3961 tggaggaagc tccggatcgc ggccaggaac cgggccatcg ctgccagcat ggtccgcggg
     4021 ggatgctgcg ggcaccacgg cgagcccggc tgctgacggg gccccgacga ggggcgcggt
     4081 cgcgcccccc acgagcaccg ctagagcctt cctcaggtcc gctcgtaggc cccgaggtcc
     4141 ggcttcccgt cccgcaggat gcctcgctga tcgcgcttcg gcgcgagctt cttcgtggcc
     4201 ctgccgatgg cgcggctccc ctgcttgagg gcgtgggtcc tggtcggccc gccgttgctg
     4261 cggagcgcct tcagcttggg gtccctcttg atcagcttcc ccagcgcgcc cgtgcgcgcg
     4321 cagcccgcca ccttctcgat cagattgcgg ccccttgagg atgatggcgt cgccctgggt
     4381 gctgcagtcg tcgtggttga tccccgccgc cgggctgccg tcgacgttgt ctccgacgat
     4441 cgtgttcgtg agccggacga ccccggcgaa cgtgggcacc gcaccggcgg gcgtcgccgc
     4501 cgcgaccagc gccagggcga cgatcgagag gacgagcgag gacacgagcg ttcgagcgat
     4561 gcgtgcggtg ccgaccacga gctatctccc atcagccgta tggggccgaa cgctagctga
     4621 ggaggtcaag gcgggcaatc ggtcggtcag ccgacgcgcg ccgcgatcgt ctccgccacc
     4681 tcgcgggtcg aggccgcgcc gcccatgtcg ggcgtgagga tgcgcctctc cgcgatgacc
     4741 gaggccaccg cggcctcgac ccgcgaggcg gcgtggtcct cggagaggtg ccgcagcatc
     4801 agggccgccg agaggatcat cgcgatcggg ttggccacgc ccttcccggc gatgtcgggg
     4861 gcgctgccgt gcaccgg
//
LOCUS       DASUTZ010000082         4810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1317416, whole genome shotgun sequence.
ACCESSION   DASUTZ010000082 DASUTZ010000000
VERSION     DASUTZ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317416"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /gene="nadC"
                     /locus_tag="VF097_03680"
     CDS             <1..584
                     /gene="nadC"
                     /locus_tag="VF097_03680"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011228350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX6261931.1"
                     /translation="LAAERTALNFLGRLSGVATQAARFVEAVGGTGVVVRDTRKTTPG
                     LRALEKYAAALGGVTPHRAGLFDGILIKDNHVAAAGGVAAAVRAAKAARPDLRVECEV
                     ETPEEAEAAVAAGAEEVLLDNMPLEAMREAVARVAGRAQLEASGGVTLENARAVAETG
                     VDALSSGAITHGGSWLDLSLEVLEDVDLPAEEG"
     gene            586..1353
                     /locus_tag="VF097_03685"
     CDS             586..1353
                     /locus_tag="VF097_03685"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008746360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX6261932.1"
                     /translation="MLLAVDVGNTETVLGVFNDRELTRHWRISTVADRTADELALMFG
                     GLLEHQDLSFDRNVTAVVVASVVPSATSALREMVRRYFGFRPLIVEPGVKTGIPVLTD
                     NPKEVGADRVLNAVAAVELHGSPAVIVDFGTATTFDAVSEKGEYVGGVIAPGVQISAA
                     ALYEHTARLPRVELVAPSSVIGKSTVESVQSGIMYGYASMVDGVVERIAKELGEPTVI
                     ATGGLAPVMIEECRSIDHHEPWLTLQGLRIVFERNTG"
     gene            1350..2828
                     /gene="lysS"
                     /locus_tag="VF097_03690"
     CDS             1350..2828
                     /gene="lysS"
                     /locus_tag="VF097_03690"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX6261933.1"
                     /translation="MTAEDPREARRRKLEALRERGVAPFALRFDRSARAADLHEKHAG
                     LEADAETGEVVRVAGRLMTLRRHGRLSFVTLRDGSGEIQLMLADGVLSEDGLALLADL
                     DLGDWVGAEGEVVTTRRGELSVRATELTLLTKALRPLPEKWHGLKDVEARYRQRYLDL
                     VANPEVAERVRQKARVLHAMREHLDGLGFVEVETPVLQPVPGGGLARPFETKHEALDV
                     HMYLRIALELHLKRLLVGGLERVYEIGRTFRNEGVSTKYNPEFTMLEAYQAFASYEEM
                     MELVEGLVKAAADAAIGTRRFTYRGTEIDLDAPWPRRTVIELVSEAAGEEVSLDTSVE
                     ELRRLAQRHGIDPHETWGPGKLILELFEKLVEETLITPTIVKDFPREVSPLARPHRDD
                     PRLTEHFDLIMGGVELAPAYSELADPDEQRARFEEQAAQKAAGEEETHPFDEEFLVAL
                     EHGMPPAGGLGLGIDRLLVILTDAPSIREVILFPALRPQPPE"
     gene            2954..4141
                     /locus_tag="VF097_03695"
     CDS             2954..4141
                     /locus_tag="VF097_03695"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6261934.1"
                     /translation="MLGVIASVTGPSRLADRPFLDGMALAEAELNEGGGVAGRPLRLE
                     VVDDAGDAAPPGRALSDVLRADGPDAILVVGEGGPIVDLRPEIQARGRPVVLLGADLY
                     SDRLLYRQVFQTSPPLRWQARSVARYLTRDRAHRRIVAVQQPGDPARSAFLDELRRQG
                     VDVEATLGLGDGRLADRAGGADALIALVDLERLDAVLRAVDRLDDPPQLVLPADLLRT
                     DLDDIPPGTVAPGPYTWAPWARPISRVRAFRSSFRRRIGRLPVASEQEGYDAVRILAA
                     GLEAGRAGDGLVRFLERHRPTGPVFSSTSVTLGPDDHLFQGELWLGLYAVPGPDERPE
                     PWLVGTRWRPIVRTFSYTGQRTLYPERDRRVFFPFWRPGRPSPRFGRSRWGIVTGPED
                     PLH"
     gene            complement(4138..>4810)
                     /locus_tag="VF097_03700"
     CDS             complement(4138..>4810)
                     /locus_tag="VF097_03700"
                     /inference="COORDINATES: protein motif:HMM:NF022059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX6261935.1"
                     /translation="TEESPGTMIFTISGDVRRPGVHELELGSSVYDLVFEVAGGPADG
                     RKVKAVFPGASSTVLSLEQIDTPLDFDSMRRVGSGLGSAGFVVYDDTTCIVRTTLTYS
                     RFLYVESCAQCPACKHGTGQITELLERIDRGEGREQDLETILARALTVTDAQRCALPT
                     GETLIVQSAVQQFWEEFAAHLGRACETDRDIPVPKYVDYDPETGFALDERYHLKRPDW
                     TYAEG"
BASE COUNT          653 a         1739 c         1750 g          668 t
ORIGIN      
        1 tcctcgccgc ggagcgcacc gccctcaact tcctggggag gctctcgggg gtggccacgc
       61 aggccgcccg tttcgtggag gcggtcggag gcaccggcgt ggtcgtccgc gacacgcgga
      121 agaccacgcc gggcctccgc gcgctggaga agtacgccgc ggcgctcggc ggagtcacgc
      181 ctcaccgagc cgggctgttc gacggcatcc tcatcaagga caatcacgtg gcggcggcgg
      241 gcggagtggc ggcggccgtc cgggcggcga aggcggcccg gcccgacctc cgcgtggagt
      301 gcgaggtcga gaccccggag gaggccgagg cggccgtggc cgccggggcc gaggaggtgc
      361 tcctcgacaa catgccgctg gaggcgatgc gggaggccgt ggcccgggtc gcgggccggg
      421 cccagctcga ggcctcgggt ggcgtgacgc tggagaacgc gcgggccgtc gcggagacgg
      481 gggtagacgc gctctcgtcc ggggcgatca cgcacggagg gtcgtggctc gacctgtcgc
      541 tggaggtgct ggaggacgtg gacctgcccg cggaggaggg ctgagatgct gctggccgtg
      601 gacgtcggca acacggagac ggtgctcggg gtgttcaacg accgcgagct gacccggcac
      661 tggcggatct cgaccgtcgc cgaccgcacc gccgacgagc tggcgctgat gttcggcggc
      721 ctcctcgagc accaggacct ctccttcgac cgcaacgtga ccgcggtggt cgtcgcgagc
      781 gtcgtgccgt ccgccacgtc ggccctccgg gagatggtgc gccgctactt cggcttccgg
      841 cccctcatcg tggagcctgg cgtgaagacc gggatccccg tgctcaccga caaccccaag
      901 gaagtggggg ccgaccgggt cctcaacgcg gtggcggcgg tggagctcca tgggtccccg
      961 gccgtgatcg tggacttcgg gacggccacg accttcgacg cggtctcgga gaagggcgag
     1021 tacgtgggcg gcgtgatcgc cccaggggtc cagatctcgg ccgccgcgct gtacgagcat
     1081 accgccaggc tcccgcgtgt ggagctggtc gcgccgagct cggtgatcgg gaagagcacc
     1141 gtggagagcg ttcagtccgg catcatgtat ggctacgcct cgatggtgga cggggtcgtg
     1201 gagcggatcg cgaaggagct cggggagccg acggtgatcg ccaccggggg gctggcgccg
     1261 gtcatgatcg aggagtgccg ctcgatcgac catcacgagc cgtggctcac gctccagggg
     1321 ctgcgcatcg tcttcgagag gaacacggga tgacggcgga ggacccgcgg gaggcgcggc
     1381 gccgcaagct cgaggccctg cgcgagcgag gcgtcgctcc gttcgcgctg cgcttcgacc
     1441 ggtccgcgcg ggcggccgac cttcacgaga agcacgccgg cctcgaggcc gacgccgaga
     1501 ccggcgaggt cgtgcgggtc gccggccgcc tcatgaccct ccgccgccac gggaggctct
     1561 cgttcgtcac gctccgcgac ggatcgggcg agatccagct gatgctggcc gacggggtcc
     1621 tctccgagga cgggctggcc ctcctcgccg atctcgatct cggagactgg gtcggggccg
     1681 agggcgaggt ggtgacgacg aggcgggggg agctgtccgt ccgggctacc gagctgaccc
     1741 tgctgacgaa ggcgctccgc ccgctgcccg agaagtggca cggcctgaag gacgtcgagg
     1801 cgaggtaccg gcagcgctat ctcgatctgg tggccaaccc ggaggtggcc gagcgggtcc
     1861 ggcagaaggc gcgcgtgctc cacgcgatgc gcgagcacct ggacgggctg ggtttcgtcg
     1921 aggtggagac cccggtgctg cagccggtgc ccggcggggg gctggccaga cccttcgaga
     1981 cgaagcacga ggcgctcgac gtccacatgt acctgaggat cgcgctcgag ctccacctga
     2041 agcggctgct cgtcggcggc ctggagcgcg tctacgagat cgggcggacg ttccgcaacg
     2101 agggcgtttc cacgaagtac aaccccgagt tcacgatgct cgaggcgtac caggccttcg
     2161 ccagctacga ggagatgatg gagctcgtcg agggactggt gaaggcggcg gccgacgccg
     2221 cgatcggtac gcggcggttc acctaccgag ggacggagat cgacctcgac gcgccgtggc
     2281 ctcggcgcac ggtgatcgag ctggtgtccg aggctgccgg agaggaggtg tcgctcgaca
     2341 cctccgtcga ggagcttcgg cggctcgccc agcggcacgg gatcgacccg cacgagacct
     2401 ggggcccggg caagctgatc ctggagctgt tcgagaagct cgtggaggag acgctgatca
     2461 ccccgacgat cgtgaaggac ttcccgcgag aggtgtcgcc gctggcgagg ccgcaccggg
     2521 acgaccccag gctgaccgag cacttcgacc tgatcatggg cggggtcgag ctggcgcccg
     2581 cgtactcgga gctggccgac cccgacgagc agcgcgcacg gttcgaggag caggccgcgc
     2641 agaaggccgc gggcgaggag gagacccatc ccttcgacga ggagttcctc gtggcgcttg
     2701 agcacgggat gcccccggcc gggggcctcg gcctgggcat cgaccgcctc ctcgtgatcc
     2761 tcaccgacgc gccctcgatc cgcgaggtca tcctcttccc cgccctccgc ccccagcccc
     2821 ccgagtgaac gcgggacgag cggcggtcgc cctcgcgctg gcgctggtcg cgtgcacgag
     2881 cccgagcccg ccgtcgctcg agccgacggc taccccgacc cccgctccga gccccacggc
     2941 ggaggacacg cacgtgctcg gggtgatcgc gtctgtgacc ggcccgagcc ggctcgccga
     3001 caggcccttc ctcgacggca tggcgctggc cgaggccgag ctcaacgagg gcggcggcgt
     3061 cgccggcagg cccctcaggc tcgaggtggt ggacgatgcc ggcgacgccg ccccccccgg
     3121 ccgggccctc tccgacgtgc tccgagcgga cggccccgac gccatcctgg tggtcggcga
     3181 gggcggcccg atcgtcgacc tccgccccga gatccaggcg cggggtcgtc ccgtcgtcct
     3241 cctcggcgcg gacctctaca gcgaccgtct cctctacaga caggtcttcc agacgagccc
     3301 tcccctgcgc tggcaggccc gctccgtggc acgctacctg acccgtgatc gcgcccaccg
     3361 gcggatcgtg gccgtccagc agcccgggga tccggcccgc tcggccttcc tggacgagct
     3421 ccggcgccag ggcgtcgacg tggaggcgac cctcggtctc ggcgacgggc gccttgccga
     3481 tcgagccgga ggggccgacg ccctgatcgc cctggtcgac ctggagcggc tcgacgcggt
     3541 cctccgagcc gtcgaccgcc tcgacgaccc tccccagctc gtcctccccg cagacctcct
     3601 gcgtacggat ctcgacgaca tcccccccgg caccgtggcc ccgggcccct acacctgggc
     3661 gccgtgggcc cgccctatct cccgcgtccg cgccttccgc tcctcgttcc gccgaaggat
     3721 cgggaggctc cccgtggcct ccgagcagga ggggtacgac gccgtgcgca tcctggccgc
     3781 cggtctggag gcgggccgcg ccggcgacgg gctcgtccgc ttcctggagc ggcaccgccc
     3841 gaccgggccc gtcttctcct cgacctcggt cacgctcggg cccgacgacc acctcttcca
     3901 gggggagctc tggctcgggc tgtacgcggt gccggggccg gatgagcgcc cggagccctg
     3961 gctggtgggc acccgctggc gcccgatcgt ccgcaccttc agctacacgg gccagcggac
     4021 gctctacccc gagcgcgacc gccgggtgtt cttccccttc tggcgtccgg gccgacccag
     4081 ccccaggttc ggcaggtcgc ggtgggggat cgtcacgggg cccgaggacc ccctccacta
     4141 gccctcggcg tacgtccagt cgggccgctt caggtggtag cgctcgtcca gcgcgaaccc
     4201 ggtctccggg tcgtagtcga cgtacttcgg caccgggatg tcccggtcgg tctcgcacgc
     4261 ccggccgagg tgagcggcga actcctccca gaactgctgg acggcgctct gcacgatcag
     4321 cgtctccccc gtcgggagcg cgcaccgctg ggcgtcggtc accgtcagcg cccgggccag
     4381 gatcgtctcc aggtcctgct cgcgcccctc cccccgatcg atccgctcca gcagctcggt
     4441 gatctgcccg gtgccgtgct tgcacgcggg gcactgggcg cacgactcga cgtacaggaa
     4501 ccgcgagtag gtcagggtcg tccggacgat gcacgtggtg tcgtcgtaga cgacgaaccc
     4561 ggccgatccc aggcccgatc cgacccgccg catcgagtcg aagtcgagcg gcgtgtcgat
     4621 ctgctccagg gagagcaccg tcgaggacgc tccggggaac accgccttca ccttccggcc
     4681 gtccgccggc ccccccgcca cctcgaagac gaggtcgtag acggagctcc ccagctcgag
     4741 ctcgtgcacc cccggcctgc gcacgtcgcc cgagatcgtg aagatcatcg tccccggcga
     4801 ctcctccgtc
//
LOCUS       DASUTZ010000083         3446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1576885, whole genome shotgun sequence.
ACCESSION   DASUTZ010000083 DASUTZ010000000
VERSION     DASUTZ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576885"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VF097_03705"
     CDS             complement(<1..351)
                     /locus_tag="VF097_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6261936.1"
                     /translation="MTDETMTLEIEDLHVEVDGKPILRGVDLRVSQGEVHAMMGPNGS
                     GKSTLASVIMGRPGYVITKGDVRFGGESITQMTPDARAKAGLFLAFQYPVEVPGVSVV
                     NFLRTAYNATKGEEM"
     gene            complement(354..677)
                     /locus_tag="VF097_03710"
     CDS             complement(354..677)
                     /locus_tag="VF097_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEX6261937.1"
                     /translation="MSLQTIGSPSEIPEGEARRYEVDGHEIAVVNLGEGEFRALGDIC
                     SHAEAYLHEGEVDVDFETIECPRHGSTFDLETGKPKSLPATTPVPAYRVKVEGDELKI
                     EIPEE"
     gene            complement(674..1858)
                     /gene="sufD"
                     /locus_tag="VF097_03715"
     CDS             complement(674..1858)
                     /gene="sufD"
                     /locus_tag="VF097_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019588136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEX6261938.1"
                     /translation="MAETFSRDALPAPGEPSWARALREEALTSFEQLPIPSQNTEEWR
                     YTDLSDFDFEAFRPHPQAAELVAPDLPEGVVFTSLDEAIERHPDLIEKALHDLVPHDR
                     TKFTALHAAFRTGGVFVHVPRNARVEVPIRATTLLERAGAAVFPHTLIVAEEGADLTF
                     VDRYVSPDLEAFSDAVTEIVVGPGARVRYVSLQDWGSGVQHLAVQRASVDRDATLHSL
                     SVAFGASLSRTEVESVLAAPGASSEMLGVYFTDDDQHFDHRSLQDHAAPNGTSDLLYK
                     GALRDESSAVYSGLIRVRPGAQKTDAMQTNRNVVLSERAKADSIPNLEIEANDVRCGH
                     AASVGPVSDEEIFYLQSRGIPREEAERLIVKGFFQEVIDRIPLPDVRDAIEEAIDEEL
                     RR"
     gene            1985..2386
                     /locus_tag="VF097_03720"
     CDS             1985..2386
                     /locus_tag="VF097_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015655223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261939.1"
                     /translation="MPEPIVYIDRSDIRQGRSEEVKAAARDLVEFIREREPQLLAYGF
                     HFDEDDTRMTVIAIHPDVASLELHMEIGSTAFRRFAELIDLRTIEVFGDPGDSVLGRL
                     REKAAGLGDGGSVVVHHQQAGFARLRVSQER"
     gene            2474..2671
                     /locus_tag="VF097_03725"
     CDS             2474..2671
                     /locus_tag="VF097_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX6261940.1"
                     /translation="MREIDRDEVRRLMDDGAHIVETLGREQWEEEHLPGAISIPLRRL
                     EDEHEQLDPSKPVVVYCWDSA"
     gene            2861..3181
                     /locus_tag="VF097_03730"
     CDS             2861..3181
                     /locus_tag="VF097_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6261941.1"
                     /translation="MADRARQAGFDTAIAINENRAVFGILRDKHLAKDPDLTVKEAMS
                     AGPSTFRLSVDPSELAQLMIDHDLPNVPITTNGGVLVGLLLREDAARAALEAHRHHEH
                     EHDG"
     gene            3159..3323
                     /locus_tag="VF097_03735"
     CDS             3159..3323
                     /locus_tag="VF097_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261942.1"
                     /translation="MSTSTTDRRRCVVCGREIEVCACCEKEDCAEAICYPDLAVRVGQ
                     SLPQPHTHGG"
BASE COUNT          522 a         1166 c         1240 g          518 t
ORIGIN      
        1 catctcctcg cccttcgtgg cgttgtaggc ggtgcggagg aagttgacga cggacacccc
       61 cggcacctcg accgggtact ggaaggccag gaagagcccg gccttggccc gcgcgtcggg
      121 tgtcatctgt gtgatggact ccccgccgaa gcgaacgtcg cccttcgtga tcacgtagcc
      181 ggggcggccc atgatcaccg acgccagcgt ggacttgccc gagccgttcg ggcccatcat
      241 ggcgtggacc tcgccctggc tcacgcgcag gtccacaccg cggaggatcg gcttgccatc
      301 cacctcgacg tgcaggtcct cgatctccag cgtcatcgtc tcgtcggtca tggtcactcc
      361 tcggggatct cgatcttcag ctcgtcgccc tcgaccttca cgcggtaggc cgggacgggg
      421 gtcgtggccg gcaaagactt cggcttgccg gtctccaggt cgaaggtgga tccgtggcgc
      481 gggcactcga tcgtctcgaa gtccacgtcc acctcgccct cgtgcaggta ggcctcggcg
      541 tgcgagcaga tgtcgcccag cgcccggaac tcgccctcgc ccaggttgac cacggcgatc
      601 tcgtggccat ccacctcgta gcgccgggcc tcgccctcgg ggatctcgct cgggctgccg
      661 atcgtctgga ggctcatcgg cgcagctcct cgtcgatcgc ctcctcgatc gcgtcccgca
      721 cgtccgggag tgggatgcgg tcgatcacct cctggaagaa gcccttcacg atgaggcgct
      781 cggcctcctc ccgggggatg ccgcggctct gcaggtagaa gatctcctcg tccgagaccg
      841 ggcccacgct ggcggcgtgg ccgcagcgga cgtcgttggc ctcgatctcg aggttgggga
      901 tcgagtcagc cttggcccgc tccgagagca ccacgttgcg gttcgtctgc atcgcgtcgg
      961 tcttctgggc gccggggcgg acgcggatca ggcccgagta cacggcgctc gactcgtcgc
     1021 ggagggcgcc cttgtacaga aggtcggagg tgccgttcgg cgcggcgtgg tcctggagcg
     1081 agcggtggtc gaaatgctgg tcgtcgtcgg tgaagtagac cccgagcatc tcggaggacg
     1141 cgccgggggc ggcgagcacc gattcgacct cggtgcgcga gaggctggcc ccgaaggcca
     1201 ccgagagcga gtggagggtg gcgtcgcggt cgaccgaggc gcgctggaca gccaggtgct
     1261 gcacgccgct cccccagtcc tggagcgaga cgtagcggac gcgggcgccc ggccccacga
     1321 cgatctcggt gaccgcgtcg gagaaggcct cgaggtcggg cgagacgtag cggtcgacga
     1381 aggtcaggtc ggcgccctcc tcggccacga tcagtgtgtg tgggaagacc gcggcgccgg
     1441 cccgttcgag cagcgtggtg gcccggatcg ggacctcgac gcgggcgttg cgggggacgt
     1501 ggacgaagac acccccggtg cggaaggcgg cgtggagcgc ggtgaacttg gtgcggtcgt
     1561 gggggacgag gtcgtggagg gccttctcga tcaggtcggg atggcgctcg atcgcctcgt
     1621 cgagcgaggt gaagacgacg ccctcgggga ggtctggggc gacgagctcg gcggcctggg
     1681 ggtgaggccg gaaggcctcg aagtcgaagt ccgagaggtc cgtgtagcgc cattcctcgg
     1741 tgttctgcga ggggatcggg agctgctcga aggaggtcag cgcctcctcg cggagggctc
     1801 gagcccagga gggctcgccc ggcgcgggca gcgcgtcacg gctgaaggtc tcggccacgc
     1861 atccagtcta acaagcgggg gtcgaatccc accgctgagg taggaattcc ctctagagag
     1921 ccctacactc gagaatcggg tcctccgggg cgagggaccg cccacgagcc cgaggggagg
     1981 gagcatgcct gagcccatcg tctacatcga ccgttccgat atacgccagg gcagatccga
     2041 ggaggtcaag gcggccgcgc gcgacctcgt ggagttcatc cgggagcgag agcctcagct
     2101 gctcgcgtac ggcttccact tcgacgagga cgacacgcgc atgacggtga tcgccatcca
     2161 ccccgacgtg gcctccctgg agctccacat ggagatcggc agcacggcct tccggcgctt
     2221 cgcggagctc atcgatctcc ggacgatcga ggtcttcggc gaccccggcg acagcgtgct
     2281 cgggcggctg cgggagaagg ccgcgggtct gggagacggg ggaagcgtcg tcgtccacca
     2341 tcagcaggcg gggttcgcgc gtctccgcgt ctcgcaggag cgatgacctg atggtcgagg
     2401 accccatcgt tgaatcaggg cggcgagacg gtccgccccc gtataggctc caagtcgaag
     2461 ggaggatccg gtcgtgcgtg agatcgatcg ggacgaggtc cggcgcctca tggacgacgg
     2521 cgcgcacatc gtcgagacgc tggggcgcga gcagtgggag gaggagcacc tgcccggcgc
     2581 gatctcgatc cccctgcgcc gcctcgagga cgagcacgag cagctcgatc cctccaagcc
     2641 cgtcgtcgtc tactgctggg actccgcctg agacctcagc ccgcgggccg cgtggcggct
     2701 cgagtcgctc ggcttcaccg aggtctacga ctatggcccc ggcaagcagg attgggtcac
     2761 ggccggcctc cccaccgacc gaggcgacga cacgccgagg gccggcgacc tggcccgggc
     2821 cgacgtcccc acgtgctccc tcgacgagcg gctgggcgac gtggccgacc gcgcgcgcca
     2881 ggccggcttc gacaccgcga tcgccatcaa cgagaaccgg gcggtcttcg ggatcctgcg
     2941 cgacaagcac ctggccaagg accccgacct cacggtgaag gaggcgatga gcgcggggcc
     3001 cagcaccttc cggctcagcg tcgacccctc cgagctggcc cagctgatga tcgaccacga
     3061 cctccccaac gtcccgatca ccacgaacgg gggcgtgctg gtcggcctcc tcctccgcga
     3121 ggacgccgcg cgggccgccc tggaggccca ccgacaccat gagcacgagc acgacggata
     3181 ggcgccgctg cgtcgtctgc ggtcgggaga tcgaggtctg cgcctgctgc gagaaggagg
     3241 actgcgccga ggcgatctgc tacccggatc tcgccgtgcg ggtcggccag tcgctccccc
     3301 agccgcacac ccacggcggc tgatctcaga cgacggcgtc ctcgtcctcc tccaggagga
     3361 cggcctgggt cggacaggcg gcgtgcgcca tccgcaggac ctcgtcctcg accgtctcgg
     3421 ggtcgagaag ctcggccttc agccgg
//
LOCUS       DASUTZ010000084        11444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_281424, whole genome shotgun sequence.
ACCESSION   DASUTZ010000084 DASUTZ010000000
VERSION     DASUTZ010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11444
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281424"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1315
                     /locus_tag="VF097_03740"
     CDS             <1..1315
                     /locus_tag="VF097_03740"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX6261943.1"
                     /translation="AVMAGVGGSSEDVAVAAQDSAFRIEGEGTGEIVEVELDAIEVEQ
                     EIAEGVTYHAWTFGGTAPGPVIRAKVGDTIRFTLNNKTNLQLSHSIDFHAAQTPWDVN
                     YQPVLPGQSLTFDWVARFPGVFMYHCGVPPVLHHIANGMYGAIIVTPADPLPELEPAR
                     EYVLVSSEFYPGDDPVKGVYEGDPAKMDAAEPGYVVFDGKANRYLDDPLVARPNELIR
                     LWVMNAGPTIDNAFHVIGALIDHVYPDGNPTNALNGMQTWSVPPGGGAMFELRIPDEG
                     QYPFVSHAFAYTGLGALGILQISEDAPPAPESYPLYGNPWDGGVTETVGTVDGDAPAQ
                     PEEEEEAAGGDGGGDGVVLTMADLAYDQTSLMVQPGDTITLVNEDPMVHDVTVDELGL
                     QATAGPQETVEFTIPDDASGTYTFYCSIPGHREAGMEGSMMIGAH"
     gene            1379..1933
                     /locus_tag="VF097_03745"
     CDS             1379..1933
                     /locus_tag="VF097_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX6261944.1"
                     /translation="MLALWIALGIVGVLLLAFVLVYNRLVRNRNRVDNAWAGIDVQLR
                     RRYDLIPNLVETVKGYAAHERKLFEEVTRARAQGIEAEGIDQQAAAENEITRGLRQLF
                     AVAENYPQLRANENFLALQEELTATESKIAFARQFYNDQVLRYNTLIQQFPSLIVARM
                     TSHTTRDFFEIEEGSRAPVEVDLS"
     gene            1939..2844
                     /locus_tag="VF097_03750"
     CDS             1939..2844
                     /locus_tag="VF097_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX6261945.1"
                     /translation="MYEQIAKNRRRSVLLVLLVLALAVALGYVLGLLFQYGIAGAIVA
                     LGIAIVMTWSSYFYGDRVVLRVSGARPAPREEYPQYHNVVEGLAIAAGIPKPDLYVVP
                     EEAPNAFATGRNPEHASIGVTEGLLETLNRVELEGVMAHELAHVRNRDILFGTLVATL
                     VGVVVLLAVFARRSIFWGGMRGGRRSGRGGGGAAGILIIVGLLLAILAPIGAQLIRLA
                     VSRRREYLADADGALLSRYPPGLASALRKISQNTKPMRVANSATAHLWLSEPSVTPGH
                     GLTRWERLFATHPPIEDRIRILEEM"
     gene            2960..3988
                     /locus_tag="VF097_03755"
     CDS             2960..3988
                     /locus_tag="VF097_03755"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HEX6261946.1"
                     /translation="MSTRGKAVLASISAVIVVAAAGIGYLMFTGQSDAIPFLGGTREP
                     ERCPLTGEEAANPAFTGRTVLAVKIENSPESRPQAGLNQADVVYEEPVEGGITRFIAL
                     YHCETAERVGPIRSARFVDPVVLRQFGPAVFAYSGGIPQVIQAVADAGLQDVGYDTNP
                     DAYTLDPSRSAPHNVYSSTRILFRSGSDRGTPPEEVFEFDEEPPAREGSRRARELHLD
                     FSPSANVYWRYQRSRDRYVRLHDETPHTSEDGEQVSAANVVVMEVELRDTGIVDAAGN
                     PSPEVVAVGSGRAWVFRNGRVIRGRWERGSEGELTRLVDRGGDVIPLAPGRTWVELFP
                     NDLEVEIG"
     gene            4042..4929
                     /gene="pdxS"
                     /locus_tag="VF097_03760"
     CDS             4042..4929
                     /gene="pdxS"
                     /locus_tag="VF097_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEX6261947.1"
                     /translation="MEKETGTFRVKSGLAEMLKGGVIMDVTNAEQAKIAEEAGAVAVM
                     ALERVPADIRAEGGVARMADPTKVEEIIEAVSIPVMAKCRIGHFVEAQILQALGVDYV
                     DESEVLTPADEANHIDKWAFTVPFVCGARDLGEALRRIGEGAAMIRTKGEAGTGNVVE
                     AVRHVRTITGQMSRLRGMRDDELMSQAKELGAPFELVRQVASTGTLPVVNFAAGGLAT
                     PADAALMMQLGVDGNFVGSGIFKSDDPAARARAIVEATTSYRDPEMLAKISRGLGEPM
                     KGIEISTLGDEGLLQTRGW"
     gene            4932..5462
                     /locus_tag="VF097_03765"
     CDS             4932..5462
                     /locus_tag="VF097_03765"
                     /inference="COORDINATES: protein motif:HMM:NF039189.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HEX6261948.1"
                     /translation="MAEERRAEGRAAYVISQLYYYVAAVIGVALLIGGAVAALFGLRE
                     LVLPREWESTEGGIRTMLTGAAFALPGLALLWTHLREARRREGRPFTETFWGASLYFH
                     LVALISLGFVLIGTIGILVSFVDVVYRPSAGVDLFQPSRSEALRQMLDFAIFVVVAGP
                     VFWWHLRRGRRAVGSP"
     gene            complement(5459..5839)
                     /locus_tag="VF097_03770"
     CDS             complement(5459..5839)
                     /locus_tag="VF097_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015936245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6261949.1"
                     /translation="MLGRYPVHATLPAADIDRARAWYEEKLDLRPTTEDPGGLWYESG
                     GVVFALFPTPHAGSATNTAMEWRVDDVPSVVEWLQGRGVEFETFEMEGVEWDGPIAKM
                     GPARGAWFKDSEGNTLAVTEGLEG"
     gene            5941..6501
                     /gene="pdxT"
                     /locus_tag="VF097_03775"
     CDS             5941..6501
                     /gene="pdxT"
                     /locus_tag="VF097_03775"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEX6261950.1"
                     /translation="MKAGILALQGDFREHGRVLASCGATPVEVRTPDDLASVECLVIP
                     GGESTTIGTLARGRGLVDPIRERAEGGMPVFGTCAGMIVMAKELIGGEPLLSLMDVTV
                     ERNAYGRQVDSFEAEVEVRGEPQPVRGVFIRAPVVVEVGEGVEVLAEHEGRPVVVEQG
                     NLLAASFHPELAPDPRLHERLLGKVR"
     gene            6501..7247
                     /locus_tag="VF097_03780"
     CDS             6501..7247
                     /locus_tag="VF097_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX6261951.1"
                     /translation="MSGHSKWSTIKHKKAAQDQRRGKLFSKLIRAIEVAAREGGGSVD
                     GNPTLASAVQKAKEYSVPNENIERAIKRGTGDLGGVRYEEVVYEGYGPGGVAILVATL
                     TDNRNRTGSDVRSTFTKRGGNLGDPGSVSFLFERKGVIRVSREEADEDRLLELVLEAG
                     AEDLVEDGNEWEVRTAPTAFADVRTAVEGAGIPISSAEVTMLPQTTVPVEGSEAKQVL
                     GLIEALDDLDDVQAVYANFDIPEEMLAEVG"
     gene            7286..8125
                     /locus_tag="VF097_03785"
     CDS             7286..8125
                     /locus_tag="VF097_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261952.1"
                     /translation="MRARPIAVLLALALGACTAPADPAPTAPSGPTYRDRVEVEAAVE
                     AFLEGFAVGAEDPAELLASVAGDRLRDWARWINRYRPEREAVRAGRVEIESLRVESIR
                     GSVAIAVVDATVHQPARDEEGNRRIVARSFNGPVELRRDASGWRVVDIVRDGAPMSYA
                     IGIFDEPAAGEEAGIRVEVASVYRFRSGTVATIRIENGTGMPIVLDRTHSLIQAAGRW
                     VGATAVAGDLDVPIPPGAAIEGSLAYHAIPIQWLPEKVFVHFLGGPEPIEVHLPRDGF
                     LPI"
     gene            8175..8672
                     /locus_tag="VF097_03790"
     CDS             8175..8672
                     /locus_tag="VF097_03790"
                     /inference="COORDINATES: protein motif:HMM:NF014166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX6261953.1"
                     /translation="MFERVVLGVDPGVASTGLAAVGGGRRRAEVIWATTVRTPAGAPE
                     AQRLRSIASAVRDAIATHRPEALAIERLLWGRNTGSAMGVARASGAVMVAGAEAGIPV
                     HEYAPLEVKMAITGDGNAGKQQVRRSLARIHRVEGVPRQPDAADAAAVALCHLHRAPL
                     AEAVR"
     gene            8669..9259
                     /gene="ruvA"
                     /locus_tag="VF097_03795"
     CDS             8669..9259
                     /gene="ruvA"
                     /locus_tag="VF097_03795"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX6261954.1"
                     /translation="MIAFVEGVLAERGADRVVVNAGGLGYELLVSTTTMAALPAIGKP
                     SRLLTHLQVRDDSMALFGFATGAERDLFELLITVSGVGPKMAMAVLSTLEPDALRRAV
                     LDGDIDAVTVVPGVGKKVAGRIVLDLRDKLGGEIDLPGTGPLAEVRQALEGMGLSPQE
                     IQGAVAELDVDERPVEELLREALRRVGGREAAGAQR"
     gene            9256..10275
                     /gene="ruvB"
                     /locus_tag="VF097_03800"
     CDS             9256..10275
                     /gene="ruvB"
                     /locus_tag="VF097_03800"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX6261955.1"
                     /translation="MTDEPFADERLVDADALDPEFDAALRPRSLDEFVGQDRIKEQLG
                     LLIAGARERGEPVDHVLFSGPPGLGKTTLAGILANEMGVALRQTSGPALERTADLAAI
                     LTNLEEHDVLFVDEIHRMPRAVEEVMYPALEEFKLDVVIGKGPSARTIRLDLPRFTLV
                     AATTRPGLITLPLRERFGFSPRLEYYGEEDLAKVVARSAGILEVEVDAEGAEEIATRS
                     RGTPRIANRLLKRVRDYAQVRADGRIDREIACDALALFEVDELGLDRVDTLILTAMIE
                     RFGGGPVGLSTLSAAVGEDAGTLEDVYEPYLLQSGFIQRTPRGRIVTEMARRHLDMPA
                     SGRLV"
     gene            10361..10690
                     /gene="yajC"
                     /locus_tag="VF097_03805"
     CDS             10361..10690
                     /gene="yajC"
                     /locus_tag="VF097_03805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX6261956.1"
                     /translation="MTVLPIAQAGGQGNVTFLISLVLLVGIFYFLLIRPQQRRMRQQR
                     ELVESLAVGDEVITIGGLYGRIMEMDDESVTVEISPGTQVRFVKSAIARRLTLEPGEP
                     DEGIIEE"
     gene            10765..11436
                     /locus_tag="VF097_03810"
     CDS             10765..11436
                     /locus_tag="VF097_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEX6261957.1"
                     /translation="MPAEASITLEEVQEHPALSAFIAQADKQMDGMGFTEHGFRHAKL
                     TGRIAYNVLDRLGYDPRTTTLGSVAGYLHDIGNCISRTDHGQTGALLINHMLGSRIEP
                     ADLAVVMGAIGNHEEEYGHAVGPVAAAVILADKSDVHRSRVRKADQIEFDIHDRVNYA
                     VEESFLRVDSDAGTITLELTIDTSISHVMEYFEIFLDRMAMCRRAAEELECRFRVVIN
                     ETEVL"
BASE COUNT         1707 a         3878 c         4223 g         1636 t
ORIGIN      
        1 cgcggtgatg gcgggcgtgg gcggctcgtc cgaggacgtc gccgtcgccg cgcaggactc
       61 ggcgttccgg atcgaggggg aggggaccgg tgagatcgtc gaggtcgagc tcgacgcgat
      121 cgaggtcgag caggagatcg cggagggggt cacgtaccac gcttggacct tcggagggac
      181 ggctcccggc ccggtgattc gggcgaaggt cggcgacacc atccgcttca cgctcaacaa
      241 caagacgaac ctccagctgt cgcactcgat cgacttccac gcggcgcaga cgccgtggga
      301 cgtcaactac cagccggtcc tgccgggcca gagcctcacc ttcgactggg tggcccggtt
      361 ccccggcgtg ttcatgtacc actgcggggt cccgccggtc ctgcatcaca tcgccaacgg
      421 gatgtacggg gcgatcatcg tgacgccggc cgaccccctg cccgagctcg agcccgcccg
      481 cgagtacgtc ctggtctcga gcgagttcta cccgggcgac gaccccgtga agggcgtgta
      541 cgagggcgat cccgccaaga tggacgcggc cgagcccggg tacgtggtct tcgacggcaa
      601 ggccaaccgc tacctggacg acccgctggt ggctcgcccc aacgagctga tccggctctg
      661 ggtcatgaac gccgggccca cgatcgacaa cgcgttccac gtgatcgggg cgctcatcga
      721 ccacgtgtac ccggacggca atcccaccaa cgcgctgaac gggatgcaga cgtggagcgt
      781 cccgccgggc ggcggcgcca tgttcgagct gcggatcccc gacgagggcc agtacccgtt
      841 cgtgagccac gcgttcgcgt acacggggct cggggcgctc ggcatcctcc agatcagcga
      901 ggacgcgccg ccagccccgg agtcgtatcc gctgtacggc aacccgtggg acggcggcgt
      961 gaccgagacg gtcggtacgg tggacggcga cgcgccggcc cagcccgagg aggaggagga
     1021 ggccgcgggc ggcgacgggg gaggcgacgg ggtcgtgctg acgatggccg acctcgccta
     1081 cgaccagacg agcctcatgg tccagccggg cgacacgatc accctggtga acgaggaccc
     1141 gatggtgcac gacgtcaccg tggacgagct cggcctccag gccaccgcgg ggccgcagga
     1201 gaccgtcgag ttcacgatcc cggacgacgc ctccggcacc tacaccttct actgctcgat
     1261 cccgggccac cgggaggcgg gcatggaggg cagcatgatg atcggggccc actgaccgga
     1321 gcggggaggg gggtcggcgc tcggcgtcga cccccccgct cgctggtacg atacgaacgt
     1381 gcttgcccta tggatcgcgc tgggcatcgt cggggtcctc ctcctggcct tcgttctcgt
     1441 ctacaacagg ctcgtccgaa accggaaccg cgtggacaac gcctgggccg gcatcgacgt
     1501 ccagctccgc cgccgctacg acctcatccc gaacctcgtg gagacggtca aggggtacgc
     1561 cgcgcacgag cgcaagctgt tcgaggaggt cacgagggcc cgggcccaag ggatcgaggc
     1621 cgaggggatc gaccagcagg cggcggctga gaacgagatc acgcgagggc tccgccagct
     1681 cttcgccgtg gccgagaatt acccgcagct ccgggcgaac gagaacttcc tggccctgca
     1741 ggaggagctg accgcgacgg agtcgaagat cgccttcgcc cggcagttct acaacgacca
     1801 ggtcctgcgc tacaacaccc tgatccagca gttcccctcg ctcatcgtgg cgcggatgac
     1861 gagccatacg acgcgcgact tcttcgagat cgaggaggga tcccgggcgc ccgtcgaggt
     1921 ggacctctcc tagcctccgt gtacgagcag atcgccaaga acaggcgccg gtcggtcctg
     1981 ctcgtcctgc tcgtcctggc gctggcggtg gcgctggggt acgtgctggg cctgctgttc
     2041 cagtacggga tcgccggcgc gatcgtcgcg ctcgggatcg cgatcgtcat gacctggtcc
     2101 tcgtacttct acggggatcg agtcgtgctg cgtgtgagcg gagcgcggcc cgccccgcgc
     2161 gaggagtacc cccagtacca caacgtcgtg gaagggctgg cgatcgcggc cgggatcccc
     2221 aagcccgacc tctacgtagt gccggaggag gcccccaacg cgttcgccac gggacggaac
     2281 ccggagcacg cctcgatcgg ggtcaccgaa gggctcctcg agacgctgaa ccgcgtggag
     2341 ctcgagggcg tgatggccca cgagctggcc cacgtgcgga accgcgacat cctcttcggg
     2401 acgctggtgg cgaccctggt cggcgtcgtc gtcctgctcg cggtgttcgc ccgtcgctcg
     2461 atcttctggg gcgggatgcg gggtggccgc cggtcgggac ggggtggtgg gggggcggcg
     2521 gggatcctga tcatcgtggg gctgctgctc gccatcctgg cgccgatcgg ggctcagttg
     2581 atcaggctcg ccgtctcccg gcggcgcgag tacctggccg acgccgacgg cgcgctcctc
     2641 agccgctacc cgcccgggct ggcctccgcg ctccggaaga tctcccagaa cacgaagccg
     2701 atgcgcgtcg ccaacagcgc cacggcccat ctctggctgt cagagccgtc cgtcacgccc
     2761 gggcacgggc tgacgcggtg ggagcgactc ttcgccacgc atcctcctat cgaggaccgc
     2821 atccgcatcc tcgaggagat gtgatcggat ctcccccact cccctggggc ggcggtcaaa
     2881 ctgaggcgag aggagtagca tctcccgccg tgcccgcagc cggggagatc gaccagggga
     2941 agatcgagga ggcttcacgt tgagcaccag agggaaggcc gtgctggcca gcatctcggc
     3001 cgtgatcgtc gtcgcggcgg ccgggatcgg atatttgatg ttcacggggc agagcgacgc
     3061 catcccgttc cttgggggga cgcgagagcc ggagcgctgc ccgctgacgg gcgaggaggc
     3121 ggcgaacccc gccttcacgg gccggaccgt gctggccgtg aagatcgaga acagccccga
     3181 gtcccggcct caggccggcc tgaaccaggc cgatgtcgtc tacgaggagc cggtcgaggg
     3241 cggcatcacg cggttcatcg ccctgtacca ctgtgagacg gccgagcgcg tcggtcccat
     3301 ccggagcgcc cgcttcgtgg accccgtggt cctccgccag ttcggcccgg cggtgttcgc
     3361 ttactcgggc ggcatccccc aggtgatcca ggcggtggcc gacgcgggcc tccaggacgt
     3421 gggctacgac acgaacccgg acgcctacac gctcgacccg agccgctcgg cgccccacaa
     3481 cgtctacagc tcgacccgga tcctgttccg ctcggggagc gaccgaggca ccccgcccga
     3541 ggaggtgttc gagttcgacg aggagccacc cgcgagggag ggctcgcgcc gcgcccggga
     3601 gctccacctc gacttctcgc cgtccgccaa cgtgtactgg cgctaccagc ggagccggga
     3661 ccgctacgtg cggctccacg acgagacgcc ccacacgagc gaggacgggg agcaggtctc
     3721 ggcggccaac gtcgtggtga tggaggtcga gctccgggac acggggatcg tggacgcggc
     3781 gggcaacccc tcgcccgagg tggtcgcggt cggatcgggg cgggcctggg ttttccggaa
     3841 cgggcgcgtg atccggggcc ggtgggagcg aggctccgag ggcgagctca cccggctcgt
     3901 ggatcgcggc ggcgatgtga tcccgctggc ccccggccgg acctgggtcg agctcttccc
     3961 gaacgatctc gaggtggaga tcggctgagc gacccgtccg ccgactcctc ggcgtatcct
     4021 gtgagggaag gaggagcggg gatggagaag gagacgggta cgttcagggt caagtcgggc
     4081 ctggccgaga tgctcaaggg cggcgtgatc atggacgtca cgaacgccga gcaggccaag
     4141 atcgccgagg aggccggcgc ggtcgcggtg atggcgctgg agcgcgtgcc cgccgacatc
     4201 cgggccgagg gtggcgtcgc tcgcatggcc gaccccacca aggtcgagga gatcatcgag
     4261 gccgtctcga tccccgtcat ggcgaagtgc cgcatcgggc acttcgtcga ggcccagatc
     4321 ctccaggcgc tgggcgtgga ctacgtcgac gagtccgagg tcctcacgcc ggccgacgag
     4381 gcgaaccaca tcgacaagtg ggccttcacg gtgccgttcg tgtgcggggc tcgcgatctc
     4441 ggtgaggcgc tgcgacggat cggggagggc gcggcgatga tccggacgaa gggcgaggcg
     4501 ggcaccggca acgtcgtcga ggcggtccgc cacgtccgca cgatcaccgg acagatgtcg
     4561 cggctgcggg ggatgcggga cgacgagctg atgtcccagg cgaaggagct gggggccccc
     4621 ttcgagctcg tgcggcaggt cgcgagcaca ggaacccttc cggtcgtcaa cttcgcggca
     4681 gggggcctgg cgacgcccgc ggacgcggcc ctcatgatgc agctcggggt ggacgggaac
     4741 ttcgtggggt ccgggatctt caagtccgac gacccggccg cgcgggcccg ggccatcgtg
     4801 gaggccacga cgtcctaccg ggaccccgag atgctggcga agatctcccg ggggctggga
     4861 gagccgatga aggggatcga gatctccacg ctcggcgacg agggtctcct ccagacccgc
     4921 ggctggtagg gatggcggag gagcggcggg ccgagggcag ggccgcctac gtcatctccc
     4981 agctctacta ctacgtcgcc gccgtgatcg gtgtggcgct cctcatcggc ggcgcggtcg
     5041 ccgccctctt cgggctccgg gagctcgtgc tcccccggga gtgggagtca acggagggcg
     5101 ggatccgcac gatgctcacg ggcgccgcgt tcgctctgcc cggtctggct ctcctctgga
     5161 cccatcttcg cgaggcgagg cggcgcgagg ggcggccctt caccgagacg ttctggggcg
     5221 cgtcgctcta cttccatctc gtggcgctga tctcgctggg gttcgtgctg atcgggacga
     5281 tcgggatcct ggtctcgttc gtggacgtgg tgtatcggcc ctcagcgggg gtggacctgt
     5341 tccagccttc ccggtcggag gcgctccgac agatgctcga cttcgcgatc ttcgtggtgg
     5401 tggccggccc cgtcttctgg tggcacctgc gacggggccg gcgcgccgtc ggatccccct
     5461 agccctccag gccctccgtc accgccaggg tgttcccctc gctgtccttg aaccaggcgc
     5521 cgcgggcggg ccccatcttc gcgatcgggc cgtcccactc gacgccctcc atctcgaagg
     5581 tctcgaactc cactccgcgg ccctggagcc actcgaccac cgagggcacg tcgtccaccc
     5641 tccactccat cgcggtgttg gtggcgctgc ccgcgtgggg cgtggggaac agcgcgaaga
     5701 ccacgcctcc ggactcgtac cagaggcctc cggggtcctc agtggtgggt cgcaggtcga
     5761 gcttctcctc gtaccaggcc cgcgccctgt cgatgtccgc ggccgggagc gtcgcgtgca
     5821 ccgggtaccg tccaagcatc tccgaacctc cttccacggg cacgctcgtc tcgtcgacat
     5881 cctgctccaa cccgatgcgc ttgggaagcc ccctgcccgc gtcgttatca tggcgatgac
     5941 atgaaggcag gcatcctcgc cctccagggc gatttccggg agcacggccg ggtcctcgcg
     6001 agctgcggcg cgacccccgt cgaggtgcgc acgcccgacg atctcgcctc ggtggagtgc
     6061 ctggtgatcc ccggcgggga gtccaccacg atcggcacgc tcgcccgggg gcggggcctc
     6121 gtggatccca tccgggagcg ggcggagggg gggatgccgg tcttcgggac gtgcgcaggg
     6181 atgatcgtga tggcgaagga gctgatcggc ggggagccgc tcctctcgct gatggacgtc
     6241 acggtcgagc gcaacgccta cgggcggcag gtagactcgt tcgaggccga ggtcgaggtg
     6301 cgcggcgagc cccagcccgt gcgcggcgtg ttcatcaggg ccccggtggt ggtcgaggtg
     6361 ggggagggcg tggaggtcct cgccgagcac gagggccgcc cggtcgtcgt ggagcagggg
     6421 aacctgctgg cggcgtcctt ccaccccgag ctcgctccgg atccgcgcct gcacgagcga
     6481 ctcctgggga aggtgcgctg atgtccgggc actcgaagtg gtccacgatc aagcacaaga
     6541 aggcggccca ggaccagcgc cgagggaagc tcttctccaa gctgatccgg gcgatcgagg
     6601 tcgccgcgcg cgagggcggc ggaagcgtgg acggcaaccc gaccctggcc tccgcggtcc
     6661 agaaggccaa ggaatactcg gtcccgaacg agaacatcga gcgggcgatc aagcggggga
     6721 ccggagacct ggggggcgtc cgctacgaag aggtcgtcta cgaggggtac ggacccggcg
     6781 gggtcgcgat cctcgtggcc acgctcaccg acaatcgcaa caggaccggc tcggacgtcc
     6841 gctccacgtt cacgaagcgc ggcggcaatc tgggtgaccc gggaagcgtg tcgttcctct
     6901 tcgagcggaa gggcgtgatc cgcgtgtccc gagaggaggc ggacgaggac cgcctcctgg
     6961 agctcgtgtt ggaggccggc gccgaggacc tggtcgagga cggcaacgag tgggaggtgc
     7021 gcaccgcccc gacggccttc gccgacgtgc ggaccgccgt cgagggcgcg ggcatcccca
     7081 tctcctcggc tgaggtcacg atgctcccgc agacgacggt cccggtggag gggtccgagg
     7141 cgaagcaggt gctggggctg atcgaggcgc tggacgatct cgacgacgtc caggccgtct
     7201 acgcgaactt cgacatcccc gaggagatgc tggccgaggt cggctgagcg gggccaagag
     7261 ccgcgctccg ggggatactc gagtcatgcg ggctcgaccc atcgccgtgc tcctggcact
     7321 cgcgctcggc gcctgcacgg cgcccgccga ccctgccccc accgccccca gcggccccac
     7381 ctaccgggac cgggtcgagg tcgaggcggc ggtcgaggcc ttcctcgagg gattcgcggt
     7441 gggcgccgag gatcccgccg agctcctggc gtcggtcgcg ggcgaccgcc tccgcgactg
     7501 ggcccgctgg atcaaccgct accgccccga gcgggaggcc gtgcgggcgg gccgcgtcga
     7561 gatcgagagc ctgcgggtcg agtcgatccg gggctcggtc gcgatcgcgg tcgtggatgc
     7621 cacggtgcac cagccggccc gggacgagga gggaaaccgc cggatcgtgg cccgctcgtt
     7681 caacggcccg gtggagctgc ggcgcgacgc ctcggggtgg agagtcgtgg acatcgtccg
     7741 ggacggagcg ccgatgagct acgcgatcgg catcttcgac gagccggcgg cgggggagga
     7801 ggccggcatc cgggtggagg tggccagcgt gtaccgcttc cggtcgggga ccgtcgccac
     7861 catccggatc gagaacggca ccgggatgcc gatcgtcctg gaccggaccc actccctgat
     7921 ccaggcggcg gggaggtggg tgggcgccac ggcggtggcc ggcgacctcg acgtcccgat
     7981 cccgccgggg gccgcgatcg agggatccct cgcctaccac gcgatcccga tccagtggct
     8041 gcccgagaag gtgttcgtcc acttcctcgg ggggcccgag ccgatcgagg tccacctccc
     8101 ccgggacggc ttcctcccga tctgaatccc gccgcttcct tgagcgggcc gcctcccgcc
     8161 cctagactga acacgtgttc gagcgggtgg tgctcggggt ggacccgggg gtggcgtcca
     8221 cgggcctcgc cgccgtgggc gggggtcggc gccgggcgga ggtgatctgg gcgacgaccg
     8281 tccgcacgcc ggcgggcgcc ccggaggcgc agcgcctgcg ctcgatcgcg agcgccgtcc
     8341 gggacgcgat cgcgacgcac cggccggagg cgctcgcgat cgagcgcctc ctctggggac
     8401 gcaacaccgg cagcgcgatg ggggtggccc gggccagcgg ggcggtgatg gtcgccggcg
     8461 cggaggcggg gatcccggtc cacgagtacg cgccgctgga ggtgaagatg gcgatcaccg
     8521 gcgacgggaa cgccgggaag cagcaggtcc gccgctccct ggcgaggatc catcgggtgg
     8581 agggcgtccc gcgccagccc gacgccgccg acgccgcggc cgtggccctg tgccacctgc
     8641 accgggcgcc gctggcggag gccgtccgat gatcgcgttc gtggagggcg tcctggccga
     8701 gcggggcgcg gaccgagtgg tcgtgaacgc gggcgggctg gggtacgagc tcctggtctc
     8761 gaccacgacg atggcggcgc tgccggcgat cgggaagccc tcgcgcctcc tgacccacct
     8821 ccaggtccgc gacgactcga tggcgctgtt cggcttcgcc acgggtgcgg agcgcgacct
     8881 cttcgagctg ctgatcacgg tctcgggcgt ggggccgaag atggcgatgg cggtgctctc
     8941 caccctggag cccgacgccc tgcggcgggc cgtgctcgac ggcgacatcg acgcggtcac
     9001 cgtggtcccg ggcgtgggga agaaggtggc cggccgcatc gtcctcgacc tgcgcgacaa
     9061 gctcggcggc gagatcgatc tcccgggcac gggccccctg gccgaggtcc gccaggccct
     9121 cgaggggatg ggcctctcgc cccaggagat ccagggcgcc gttgcggagc tcgacgtcga
     9181 cgaacgcccg gtggaggagc tgctgcgcga ggcgctgcgc cgggtgggcg gtcgggaggc
     9241 cgccggagcg cagcgatgac cgacgagccc ttcgcggacg agcggctggt ggacgccgac
     9301 gcgctcgacc cggagttcga cgcggcgctc cgtcctcgat cgctcgacga gttcgtgggg
     9361 caggaccgga tcaaggagca gctggggctc ctgatcgccg gggcccgcga gcgtggggaa
     9421 ccggtggacc acgtgctgtt ctcgggcccg ccgggcctgg ggaagacgac gctggccggc
     9481 atcctggcga acgagatggg ggtggccctg cggcagacct cggggcccgc gctggagcgg
     9541 accgccgacc tcgctgcgat cctcacgaac ctcgaggagc acgacgtgct gttcgtggac
     9601 gagatccacc ggatgcctcg agcggtggag gaggtcatgt atccggcgct cgaggagttc
     9661 aagctcgacg tggtgatcgg gaagggcccc agcgcccgca cgatccggct cgatctccct
     9721 cggttcacgc tcgtggccgc gacgacccgc ccggggctca tcaccctgcc cctacgagag
     9781 cgattcgggt tctcgccgcg actcgagtac tacggggagg aggacctggc caaggtcgtg
     9841 gcccgctcgg cggggatcct cgaggtggag gtcgacgccg aaggtgccga ggagatcgcg
     9901 acgcgctccc gggggacgcc ccggatcgcg aaccgcctcc tgaagcgggt ccgggactac
     9961 gcgcaggtcc gcgccgacgg gcggatcgac cgtgagatcg cctgcgacgc cctggccctc
    10021 ttcgaggtgg acgagctcgg gctcgaccgc gtggacacgc tgatcctcac ggcgatgatc
    10081 gagcgcttcg ggggaggccc ggtggggctc tccacgctgt cggccgccgt gggcgaggac
    10141 gctggcaccc tcgaggacgt ctacgagccg tacctcctcc agagcggctt catccagcgc
    10201 accccgaggg gccggatcgt caccgagatg gcccgccgcc atctggatat gccggcttcc
    10261 ggccgcctgg tctgaccgcc ctgcgtcgct agccccggtc cgatagaatc gcggccggac
    10321 acccggcggc gaggccgccg tttttcatgg aggcggacac atgacagtcc taccgatcgc
    10381 acaggccggc ggccagggga acgtaacgtt cctgatctcg ctcgtcctcc tggtggggat
    10441 cttctacttc cttctgatcc ggccccagca gcgcaggatg cgccagcagc gagagctcgt
    10501 ggagagcctg gcggtcgggg acgaggtcat cacgatcggc ggcctgtacg gtcgcatcat
    10561 ggagatggac gacgagtccg tcaccgtcga gatcagcccc ggcacgcagg tgcggttcgt
    10621 gaagagcgcg atcgcccgac gcctgacgct cgagcccggg gagccggacg aagggatcat
    10681 cgaggagtga gcgaccgcgg gccccgtcgg gaggcggcgg cgtcgggcgc caagcgcgac
    10741 gccgcctctc gccgggagca gaaggtgccc gccgaagcgt cgatcacgct ggaggaggtc
    10801 caggagcacc cggccctctc cgcgttcatc gcccaggccg acaagcagat ggacggcatg
    10861 ggcttcaccg agcacggatt ccgccacgcg aagctcacgg gcaggatcgc ctacaacgtg
    10921 ctcgaccgcc tcgggtacga tccccggacg acgaccctcg ggtccgtagc cggctacctg
    10981 cacgacatcg ggaactgcat ctcccgcacg gaccacgggc agacgggcgc gctgctgatc
    11041 aaccacatgc tcgggagcag gatcgagccc gcggatctgg ccgtggtgat gggtgcgatc
    11101 ggcaaccacg aggaggagta cgggcacgcg gtcgggcccg tcgccgcggc cgtgatcctc
    11161 gccgacaagt cggacgtgca ccgatcccgc gtccgcaagg cagaccagat cgagttcgac
    11221 atccacgacc gcgtgaacta cgcagtggag gagtcgttcc tccgcgtgga ttcggacgcc
    11281 ggcacgatca ccctggagct gacgatcgac acgtcgatca gccacgtgat ggagtacttc
    11341 gagatcttcc tcgaccggat ggcgatgtgc aggagggcgg cggaggagct cgagtgccgc
    11401 ttccgcgtcg tgatcaacga gaccgaggtg ctgtgatcgg gagg
//
LOCUS       DASUTZ010000085         3361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1449199, whole genome shotgun sequence.
ACCESSION   DASUTZ010000085 DASUTZ010000000
VERSION     DASUTZ010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449199"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1029)
                     /locus_tag="VF097_03815"
     CDS             complement(1..1029)
                     /locus_tag="VF097_03815"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HEX6261958.1"
                     /translation="MAVVAGGLAYVQLEKKVTLVVDGEERRVSTFASSVGDLLEGTGI
                     EIREHDEVSPPLDAPVADDMRVEILLAKEITLVLNGKPRTLYVTGQTVEDVLAHVNLR
                     SANKAYVRPSRGAAISDGDRIVLREAVEVRVKVDGADRQVITNAPDVGHLLVSLGVIL
                     RKNDEIEPGLDRRLTNGLTIQVTRVTFKQTTVREGIPFGTEDRYSDEFLKGVTHVERE
                     GAPGVREKVFRVRMEDGKEAARTLVSERVVEQPVSRIVIHGTRDPRVQTGLASWYHRS
                     GMVAAHRTLPFGTRVKVTNLGNGRSVWVTIDDRGPYIDGRIIDLSDDAFLQLAPLGAG
                     TINVRITW"
     gene            complement(1222..1977)
                     /locus_tag="VF097_03820"
     CDS             complement(1222..1977)
                     /locus_tag="VF097_03820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX6261959.1"
                     /translation="MIDTHCHLFLMEGEPNELVREAEEAGVGTLVCVGVDRESSHRSR
                     ELADSLRGVFATAGVHPHDAKDFDDATGVEIEELLSDPRVVAVGETGLDHYRMLSPAK
                     DQERVFRAHCSLAREHDKPVVVHTRDAWPDVLRILEEERLERVILHCFSGGEEEAREA
                     ASRGYHCSFAGPVTYPKNERLRAAAARVPEELLLVETDSPYLPPQPLRGRPNVPGSLR
                     LTLQAVAEARGQGLEALEEITERNARAAFSLPE"
     gene            complement(1981..>3361)
                     /gene="metG"
                     /locus_tag="VF097_03825"
     CDS             complement(1981..>3361)
                     /gene="metG"
                     /locus_tag="VF097_03825"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX6261960.1"
                     /translation="GKKVPQAAEEHGLDPQAFVDQMEPRWREVWQRLDIAHDDYIRTT
                     EPRHEAAVAKLLQAVYENGRDDIYLGSYEGLYCVSCEAYYTEDELVDGNCPIHGRPVE
                     AMSEENYFFRLSAYADRLLEHYERHPEAVEPETRRNEVLSTIRQGLRDFSISRTSFAW
                     GVPLPWDPDHVCYVWFDALTNYITAAGFADDPERFERMWPADVHFIGKDILRFHAIYW
                     PAMLMAAGVEPPKQVWAHGFLLVGGEKMSKTKLTGIHPFELVDHFGVDAYRWYFLREV
                     AFGQDGSFSWESMVARTNADLANGLGNLASRVLAMIASSFEGGVPEPEAEGLDGGLPG
                     TIEEVVARYDRLVDEMELTAALGAVWEIVGRANEYLVEVEPWKLAKDPDRRDETATIL
                     YTAAETLRILAVLISPIMPSAAERLWSQLGIPEPLEDQRLPEAGRWGGLAPGTRVTKG
                     EALFPRLEG"
BASE COUNT          475 a         1191 c         1161 g          534 t
ORIGIN      
        1 ctaccaggtg atccggacgt tgatcgtccc cgcgccgagg ggggcgagct ggaggaaggc
       61 gtcgtcggag aggtcgatga tccgtccgtc gatgtacggt cctcggtcat cgatcgtcac
      121 ccagacgctt ctcccgttgc ccaggttcgt caccttgacc cgcgtcccga acgggagggt
      181 ccggtgcgcg gccaccatcc ccgaacggtg gtaccacgag gcgaggcccg tctgcaccct
      241 ggggtcgcgg gtcccgtgga tcacgatccg ggagacgggc tgctccacga cccgctcgga
      301 gacgagcgtt cgggcggcct ccttcccgtc ctccatgcgg acccggaaca ccttctcccg
      361 gacccccggc gctccctcgc gctccacgtg ggtcacgccc ttcaggaact cgtccgaata
      421 ccggtcctcg gtcccgaaag ggatcccctc gcggaccgtc gtctgcttga aggtcacccg
      481 cgtgacctgg atcgtcaagc cgttggtgag gcgccggtcg aggcccggct cgatctcgtc
      541 gttcttccga aggatcacgc ccaggctgac gagcagatgg cccacgtccg gggcgttcgt
      601 gatgacctgg cggtcggcgc cgtccacctt gacccgcacc tccacggcct cgcgcagtac
      661 gatgcgatcg ccgtccgaga tcgcggcgcc gcgcgacggg cgcacgtagg ccttgttcgc
      721 cgagcggagg ttcacgtggg ccagcacgtc ctcgacggtc tgccccgtca cgtagagggt
      781 ccgcggcttc ccgttcagca cgagcgtgat ctccttggcc aggaggatct cgaccctcat
      841 gtcgtcggcg accggcgcgt ccaggggcgg ggagacctca tcgtgctccc ggatctcgat
      901 gcccgtgccc tcgagcagat cgcccaccga cgaggcgaag gtgctgacgc ggcgctcctc
      961 cccgtccacc acgagcgtca ccttcttctc gagctggacg taggccaggc cgccggccac
     1021 gacggccacg acgaggagca ggcgcccgaa tcccctggga cggggagcac gccgacctac
     1081 gcgcgcggtg cgcctgctcg ccctacggtt ctcggcgcgc ggtggggaag ggagccgcgc
     1141 gctccgtgat gctcctccct caggcaaacc gcgaccgagg ctagcaaagg accctggctg
     1201 ttgccagtta ccggatccgc gctactcggg gaggctgaag gccgcgcggg cgttgcgctc
     1261 ggtgatctcc tcgagcgcct cgagcccctg acctctggct tcggccacgg cctggagcgt
     1321 caggcgcagg cttccgggga cgttgggccg cccccggagc ggctgcggcg ggagatatgg
     1381 gctgtcggtc tccacgagga ggagctcctc tggcacgcgg gcggccgccg ctcgaaggcg
     1441 ctcgttcttc gggtaggtga ccgggccggc gaacgagcag tggtagccgc ggctggctgc
     1501 ctccctcgcc tcctcctcgc cgcccgagaa gcagtggagg atcacgcgct cgagccgctc
     1561 ttcctccagg atccggagga cgtcgggcca cgcgtcccgt gtgtgcacga ccacgggctt
     1621 gtcgtgctcc cgggccagcg agcagtgggc ccggaagacc cgttcctggt ccttcgccgg
     1681 cgagagcatc cgatagtggt cgaggcccgt ctcgcccacg gccacgacgc ggggatcgct
     1741 gaggagctcc tcgatctcga cgccggtggc gtcgtcgaag tccttcgcgt cgtgggggtg
     1801 gacccccgcg gtggcgaaga cgccgcgcag cgagtcggcc agctcacggg accggtgcga
     1861 cgactcgcga tccacgccca cgcagacgag cgttcccacg cccgcctcct cggcctcgcg
     1921 caccagctcg ttgggctctc cctccatcag gaacaggtgg caatgggtgt cgatcacggg
     1981 tcatccctcg aggcggggga agagggcctc ccctttcgtc acgcgggtgc cgggggcgag
     2041 gccgccccag cgacccgcct cggggaggcg ctgatcctcg agcggctcgg ggattccgag
     2101 ttggctccag agacgctcgg cggcgctggg catgatcggc gagatcagca ccgcgaggat
     2161 ccgcagcgtc tcggcggccg tgtagaggat cgtggcggtc tcgtcgcggc ggtcggggtc
     2221 cttcgccagc ttccacggct cgacctcgac caggtactcg ttggcccgtc ccacgatctc
     2281 ccagaccgcg ccgagggccg cggtcagctc catctcgtcc acgaggcgat cgtagcgggc
     2341 gaccacctcc tcgatcgtgc ccggcagccc cccgtcgagc ccctcggcct ccggctcggg
     2401 gacgcctccc tcgaacgagc tggcgatcat ggccaacacg cggctggcga ggttgcccag
     2461 cccgttggcg aggtcggcgt tcgtgcgcgc caccatcgac tcccacgaga acgagccgtc
     2521 ctggccgaag gccacctccc gcaggaagta ccagcggtag gcgtccaccc cgaagtggtc
     2581 cacgagctcg aaagggtgga tgccggtgag cttcgtcttc gacatcttct cgccgcccac
     2641 gagcaggaag ccgtgggccc agacctgctt cggaggctcc acgcccgccg ccatcagcat
     2701 cgccggccag tagatcgcgt ggaagcggag gatgtccttc ccgatgaagt ggacgtcggc
     2761 cggccacatc cgctcgaacc gctcggggtc gtcggcgaac ccggcagcgg tgatgtagtt
     2821 cgttagggcg tcgaaccaca cgtagcacac gtggtccggg tcccatggga ggggcacgcc
     2881 ccacgcgaac gaggtgcgcg agatcgagaa gtcgcggagc ccctgccgga tcgtggagag
     2941 cacctcgttg cgccgcgtct cgggctccac ggcctcgggg tggcgctcat agtgctcgag
     3001 caggcggtcg gcgtaggccg aaagacggaa gaagtagttc tcctcggaca tcgcctcgac
     3061 gggccgcccg tggatcgggc agtttccgtc cacgagctcg tcctccgtgt agtaggcctc
     3121 gcacgagacg cagtagagcc cctcgtagct ccccaggtag atgtcgtcgc gcccgttctc
     3181 gtacacggcc tggagcagct tggcaacggc ggcctcgtgg cggggctcgg tcgtgcggat
     3241 gtagtcgtcg tgggcgatgt cgaggcgctg ccagacctcg cgccaccggg gctccatctg
     3301 gtcgacgaag gcctgggggt cgaggccgtg ctcctcggcg gcctggggca ccttcttccc
     3361 g
//
LOCUS       DASUTZ010000086         2470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1707620, whole genome shotgun sequence.
ACCESSION   DASUTZ010000086 DASUTZ010000000
VERSION     DASUTZ010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1707620"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..617)
                     /locus_tag="VF097_03830"
     CDS             complement(162..617)
                     /locus_tag="VF097_03830"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEX6261961.1"
                     /translation="MGASAKDRALRLLGVRSRSREELRRRLKRAGFPEDEIAEALGDL
                     ERVGLVDDERFADEVVRWRLSRQGYGHRAALDTLRKAGVDRGVAERAVSAAGWEDDEA
                     RAEEVGRARLARLEGLAPDAAYRRLMGFLLRRGFDPEVVRTVCGRLLRE"
     gene            complement(619..>2470)
                     /locus_tag="VF097_03835"
     CDS             complement(619..>2470)
                     /locus_tag="VF097_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019735100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261962.1"
                     /translation="ARTLGVNIDELLVSQPDSGEQALEIADMLVRSGALDLIVVDSVA
                     ALVPRAEIEGEMGDAHVGLQARLMSQAMRKLSGSLSRFETTAVFINQLREKIGVMFGC
                     LSYGTRVTLADGTQEKIGKIVNQRMPVEVLSYDPERGEVVPRRVVSWFDNGRTDRFLQ
                     FTVARASGNGRSQFACTPNHKIRTPGGWKEARELRIGDRVMQAAPHRLSDFQWQAILG
                     GLMGDGALSPTRTAGGARFRWGHGARQVEYADWKASLFANVGTSRSTNADGAVFHDLS
                     PLPELKELREAVYVGGKKVLSEDYLKRLTPLSLAIWYMDDASFSVRSKGLQERTRGGS
                     GRAEICVEAMEVTSRERLRAYLADGWGIGSKLLQRAGKAVLQFPTAETAKLQALIAPF
                     VHPSMAYKLLPRFRGRFSAEPVFVPARHELMPMPIVDIHDKPRGGSTHRFDIEVDGSH
                     NYLADGVVVHNSPETTPGGRALKFYSSVRLDIRKIDNLKDGTEVVGSRVRVKVVKNKV
                     APPFRLAEFDIVYGKGISREGSLIDMGVAHDIVRKSGAWFTYGDEQLGQGRENARQFL
                     QEHADIALEIERRVREAVGMTDFAEDGDEVVDLDGEAQAEESEEAKAPAG"
BASE COUNT          338 a          911 c          817 g          404 t
ORIGIN      
        1 cctcggaggg cgatggggac tacggggacg ccgcgcgcgg cctctgggtc tcctcgctgg
       61 tcggggtggg gcgttccatc gtctccgtgt tcgctctctc gagggttggt tgtcgtcctg
      121 cgttcacaag ggtacgacct cgctccggcc cggcgcaaga gctactctcg gaggagcctc
      181 ccgcagacgg tccgcaccac ctcgggatcg aagcctcgcc tcaggaggaa ccccatcagg
      241 cggcgatagg ccgcgtccgg ggcgagcccc tcgagccggg cgaggcgggc ccggcccacc
      301 tcctcggccc gggcctcgtc gtcctcccag ccggcggccg acaccgcccg ctcggcgacc
      361 cctcgatcca cgcccgcctt ccggagggtg tccagcgcgg cacggtgccc gtagccctga
      421 cgagagagcc tccatcggac gacctcgtcg gcgaatcgct cgtcgtccac gagacccacg
      481 cgctcgaggt cgcccagcgc ttccgcgatc tcgtcctcgg ggaacccggc ccgcttcagg
      541 cgccgccgca gctcctcgcg gctcctggaa cggaccccga ggagcctgag ggcccggtcc
      601 ttcgcgctcg cgcccacgtc agccggccgg ggccttcgcc tcctccgact cctcggcctg
      661 ggcctcgccg tccaggtcca ccacctcgtc gccgtcctcg gcgaagtcgg tcatccccac
      721 cgcctcccgc accctccgct cgatctcgag cgcgatgtcg gcgtgctcct ggaggaactg
      781 gcgggcgttc tcacggcctt ggcccagctg ctcgtctccg taggtgaacc acgcgcccga
      841 cttccggacg atgtcgtgag ccacccccat gtcgatgagg ctgccctcgc gtgagattcc
      901 cttgccgtag acgatgtcga actcggcgag tcggaagggt ggggccacct tgttcttcac
      961 gaccttcacc ctgacccgcg atccgacgac ctcggtcccg tccttgaggt tgtcgatctt
     1021 acggatgtcg agtcggacgc tggagtagaa cttcagggcg cggcccccgg gggtggtctc
     1081 gggactgttg tgcaccacga cgccgtcggc aaggtagttg tggctcccat cgacctcgat
     1141 gtcgaaccgg tgcgtgctcc cacccctcgg cttgtcgtgg atgtcgacaa tcggcatcgg
     1201 catcagctcg tggcgcgccg gcacgaagac cggctctgcg gagaagcgcc cccggaaccg
     1261 cgggagcagc ttgtaagcca tcgagggatg gacgaacggc gcgatcagcg cttggagctt
     1321 ggcggtctcc gccgtgggga actgcaggac ggccttcccc gcccgctgga gcagcttgga
     1381 gccgatgccc catccgtcgg cgaggtacgc acggagccgc tcgcggctcg tgacctccat
     1441 cgcctcgacg cagatctcag ctcggccgct gccgccgcgg gtgcgctcct ggaggccctt
     1501 gctgcggacg gagaacgagg cgtcgtccat gtaccagatc gccagcgaga gcggcgtgag
     1561 gcgcttgagg tagtcctcgc tgagcacctt cttgcccccg acgtacaccg cctcccgcag
     1621 ctccttcagc tccgggagcg gcgagaggtc atggaacacc gcgccgtccg cgttcgtgga
     1681 gcggctcgtc ccgacgttcg cgaacagcga ggccttccag tccgcgtact cgacctgtcg
     1741 cgcaccgtgc ccccaccgga accgggcgcc gccggcggtc ctcgtcgggg agagcgcccc
     1801 gtcgcccatc agcccaccca ggatcgcctg ccactggaag tccgacagcc gatgcggggc
     1861 ggcctgcatc acccggtcgc cgatccgcag ctcccgcgcc tccttccatc cgccgggcgt
     1921 cctgatcttg tggttcggcg tgcaggcgaa ctgcgacctg ccgttccccg aggcccgcgc
     1981 cacggtgaac tggaggaagc ggtcggtcct cccgttgtcg aaccagctca cgacccgtct
     2041 ggggacgacc tcgcctcgtt cggggtcgta ggagagcacc tccaccggca tccgctggtt
     2101 gacgatcttt ccgatcttct cctgggtgcc gtcggcgagc gtcaccctcg tgccgtagga
     2161 gaggcagccg aacatcacgc cgatcttctc gcggagctgg ttgatgaaga ccgcggtcgt
     2221 ctcgaagcgg gagagggagc cggagagctt ccgcatggcc tgggacatca gccgggcctg
     2281 gagcccgacg tgggcgtcgc ccatctcgcc ctcgatctcg gcgcgaggga cgagcgccgc
     2341 caccgagtcc accacgatga ggtcgagcgc gccggagcgg acgagcatgt ccgcgatctc
     2401 gagggcctgc tcccccgagt cgggctggga gacgaggagc tcgtcgatgt tcacgcccag
     2461 cgtcctggcg
//
LOCUS       DASUTZ010000087         3193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1579623, whole genome shotgun sequence.
ACCESSION   DASUTZ010000087 DASUTZ010000000
VERSION     DASUTZ010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579623"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1366
                     /locus_tag="VF097_03840"
     CDS             179..1366
                     /locus_tag="VF097_03840"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX6261963.1"
                     /translation="MKRLFLIVCALAVSLPLPAADAGPQQQLDRIQDELERQRELLQQ
                     TNQEREGLLDDISDADRRRGDLTAKIEALQGDLARAEARLQEAQASLDLARASLRRWT
                     NRLEGARDRLTAQRDTLSSRAAAAYMMGAGGLVEVILGAEDLRSLTDRLEYVRSVMDS
                     DSYLLSSIQATRVQVAERRDHVADQESVLSEHHDAIQQEVERIAQLKAEQEALRAEVD
                     AAIAEREQLLEGVEENRAQYVKAVKELEAESARIAGLIQGGGSSGSGNPNAQLFWPAP
                     GSITSGFGWRVHPIFGTRRFHSGVDIDAGCGDPIWAAERGTVISAGWSGGYGNATVID
                     HGDGLATLYAHQESIGVGVGQRVGRGQSIGRVGTTGWSTGCHLHFEVRVNGTPVDPVP
                     YLS"
     gene            1399..2520
                     /locus_tag="VF097_03845"
     CDS             1399..2520
                     /locus_tag="VF097_03845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX6261964.1"
                     /translation="MNQVRPVFIGALIVTVLAAGTFVAGTRLGDPRSSDWDVLEETAD
                     ELRREAARSVSERELVRAAIEGMLTVLDDPYAASLDPDQADGVRDLIDGSIIGIGVWL
                     RESPQGLRVTGVVEGGPAQRAGLAEGDVIVEAEGRSMEDVSVSDAAAVFTGKAGSRME
                     LVVEREGETRTVRVERARIEIPDVEARMIDGAGYARLRQFGRGAADELGEAVRDLLER
                     GARGMVLDLRGNAGGLADEAFGVADLFLDGGVVARIRERGEPERTERAGHEHLGSFPL
                     VVLVNGDTASASELVAGALQDRDRATLVGSTTFGKGSVLTVHEVSDDTTIQYTTAFFF
                     TPDGHAIEGQGVSPDVEVESSRGQDGDPQLDRALRVLGP"
     gene            2525..2989
                     /gene="smpB"
                     /locus_tag="VF097_03850"
     CDS             2525..2989
                     /gene="smpB"
                     /locus_tag="VF097_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014911332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX6261965.1"
                     /translation="MTKGQKTVATNRRARFDYEIDERYEAGIVLTGSEVKSLRGGKAS
                     LSEAYGRITDDEVWLEGLHIPPYAQDSTRREYDPRRPRKLLLRRVEIERLKGKLNERG
                     YTLVPMRVYFSHGLAKLEVGVGRGKRRYEKRQAIAEREHKREIEREMARRNR"
     gene            3023..>3193
                     /gene="ssrA"
                     /locus_tag="VF097_03855"
     tmRNA           3023..>3193
                     /gene="ssrA"
                     /locus_tag="VF097_03855"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          481 a         1082 c         1182 g          448 t
ORIGIN      
        1 gtggttcggg acgagccata tcctcttcac cgtccccatc ctgttggtcg cgggcgtgct
       61 gatcgccacg gtggcgagcc tgttcgccat gcggcggttc ctcgaggtct gaggggtcct
      121 tggccggctc ggggccatcc gttatcatca tcgtcccccc tgaatcggat ccgatctgat
      181 gaagcgtctc ttcctcatcg tgtgcgctct ggccgtttcc cttcctctgc cggcggcgga
      241 cgcggggcca cagcagcagc tcgaccgcat ccaggacgag ctggagcgcc agcgggagct
      301 gctccaacag acgaaccagg agcgcgaggg cctgctcgac gacatctcgg atgccgaccg
      361 gcgtcggggt gacctcacgg cgaagatcga ggcccttcag ggcgacctgg cccgggccga
      421 ggcgcgcctc caggaggccc aggcctcgct cgatctggcg cgggcctcgc tccgccgctg
      481 gaccaaccgc ctggagggcg cccgggaccg gctgacggcc cagcgcgaca cgctctcctc
      541 ccgcgctgcg gccgcctaca tgatgggcgc cggcggcctc gtggaggtga tcctgggggc
      601 cgaggatctc cgctccctga ccgatcgcct cgagtacgtg cgcagcgtca tggacagcga
      661 ctcctacctt ctcagctcga tccaggccac gcgggtgcag gtggccgagc ggcgggacca
      721 cgtcgcggac caggagtcgg tcctctcgga gcatcatgac gcgatccagc aggaggtcga
      781 gcggatcgcg cagctgaagg cggagcagga ggccctccgg gccgaggtgg acgcggcgat
      841 cgccgagcgg gagcagctgc tggagggggt cgaggagaac cgcgctcagt acgtcaaggc
      901 ggtcaaggag ctcgaggccg agtcggccag gatcgccggc ctgatccagg gcggggggtc
      961 gtcgggctcc ggcaacccga acgcccagct gttctggccg gcgcccggct cgatcacgtc
     1021 gggcttcggg tggcgggtcc atccgatctt cggcacgcgc aggttccatt ccggggtgga
     1081 catcgacgcc ggctgcggcg acccgatctg ggcggccgag cggggcacgg tgatctcggc
     1141 ggggtggagc gggggctacg gcaacgccac cgtgatcgac cacggggacg gcctggccac
     1201 gctctacgcg caccaagaga gcatcggggt cggggtcggg cagcgcgtgg gacggggcca
     1261 gtcgatcggc cgagtgggca ctacgggatg gtccaccggg tgtcacctcc acttcgaggt
     1321 ccgcgtcaac ggcacgcccg tcgaccccgt gccctacctg agctgagtcc cgcggcgagc
     1381 gggtaccctg aaggcatcgt gaaccaggtc cgccccgtct tcatcggcgc gctcatcgtc
     1441 acggtcctcg cggccgggac cttcgtcgcc ggcacccgcc tgggggaccc tcgctcctcg
     1501 gattgggacg tcctggagga gaccgccgac gagctgcggc gggaggccgc ccgctccgtc
     1561 tccgagcggg agctggttcg agcggccatc gaggggatgc tgacggtcct cgatgacccc
     1621 tacgctgcct cgctcgaccc cgaccaggcc gacggggtcc gggacctgat cgacggcagc
     1681 atcatcggga tcggggtgtg gctccgtgag tccccccagg gcctcagggt caccggggtc
     1741 gtggagggcg ggccggccca gcgggccggg ttggccgagg gcgacgtcat cgtcgaggcc
     1801 gagggccgct cgatggagga cgtcagcgtg agcgacgcgg cggcggtctt caccgggaag
     1861 gccggatcgc gcatggagct cgtggtggag cgcgagggtg agactcgcac ggtccgcgtc
     1921 gagcgggcca ggatcgagat ccccgacgtc gaggcccgca tgatcgacgg agcgggctac
     1981 gcgcgccttc gccagttcgg gcggggagcg gcggacgagc tgggcgaggc cgtccgggat
     2041 ctgctcgaac ggggcgcccg agggatggtg ctcgaccttc gagggaacgc aggcgggctg
     2101 gccgacgagg ccttcggcgt ggccgacctc tttctcgacg ggggcgtcgt ggcccggatc
     2161 cgcgagcgcg gggagcccga gcggacggag cgcgccggcc acgagcatct cggctcgttc
     2221 ccgctcgtgg tcctggtcaa cggggacacc gcctcggcgt cggagctcgt ggccggcgcg
     2281 ctccaagacc gggaccgtgc caccctcgtg ggctccacca ccttcgggaa gggctcagtg
     2341 ctgactgtgc acgaggtctc ggacgacacc acgatccagt acacgaccgc cttcttcttc
     2401 acccccgacg gccacgcgat cgaggggcaa ggggtgagtc ccgacgtcga ggtcgaatcc
     2461 agccgcggcc aggacgggga tccgcagctg gaccgggccc tccgggtcct cggcccctga
     2521 cgcgatgacc aagggccaga agaccgtcgc cacgaaccgc cgggcccgct tcgactacga
     2581 gatcgacgag agatacgagg ccggcatcgt cctcaccggg tccgaggtga aatccctgcg
     2641 aggagggaag gcgtcgctgt ccgaggccta cggccgcatc accgacgacg aggtgtggct
     2701 ggagggcctc cacatccccc cgtacgccca ggactcgacg cgccgcgagt acgacccccg
     2761 ccgccctcgc aagctcctgc tgcggcgcgt ggagatcgag cgcctcaagg ggaagctcaa
     2821 cgagcgcggc tacacgctcg tgccgatgcg cgtgtacttc tcgcacggtc tggccaagct
     2881 cgaggtcggt gtgggccgcg ggaagcgccg gtacgagaag cgccaggcga tcgccgagcg
     2941 cgagcacaag cgcgagatcg agcgcgagat ggcccgccgc aatcgctgac cagcgccgat
     3001 atactgggag gcgaactgca tagggggtga ccggcttcga cccgggtgga gaaccgtccg
     3061 ggggaagcga gccgaggtcc ccggagtcct cgttaaagag ccgggaccaa gataagcgcc
     3121 gattctgaac tcgcgcttgc tgcctaaata gcagccgtcc gccgcggagc gtctgcggcc
     3181 gcgggccggg cgt
//
LOCUS       DASUTZ010000088         3849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_413281, whole genome shotgun sequence.
ACCESSION   DASUTZ010000088 DASUTZ010000000
VERSION     DASUTZ010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413281"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="VF097_03860"
     CDS             <1..931
                     /locus_tag="VF097_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261966.1"
                     /translation="DAEIQDLLGEQVGGFYDSDTGELVVADDAREGLTTVELLTLVHE
                     LQHALADQRLSTPDLETLNEEDQDAATAAQALVEGDAQLTTEIYAVRSLSTTDQLAAA
                     SQQVPGLGDTPHFLARSLLFPYLEGAQFACHLYREGGWEAVDAAYDDLPRSSAEILFP
                     DRYGSAQGPVNPTDPRGLPAPWEEEATFTFGAADLLFLFEAPGGDATNGLTEARDRVR
                     SWTGGEAHVWAWGGRTAVGLVLAASDGEAMCESVATWWRRAAEDAAEATEEVPGAALA
                     TDGRSRDGVLYCEDGSVRLGVGPDLAVARAAVR"
     gene            946..1368
                     /locus_tag="VF097_03865"
     CDS             946..1368
                     /locus_tag="VF097_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261967.1"
                     /translation="MIRRLLWVMVLLAAGAAAGYVWRDARPQAAAPRNAPPVVEQNDP
                     DPGGPPVVWLSGTLRDVTEERLTLQEGVGPTLDLTRFAGGATRFHRLGPGGWARVPRE
                     EADAAAGQDACIEALLDQGELLAIRVFLGATCSPAVPG"
     gene            1378..1617
                     /locus_tag="VF097_03870"
     CDS             1378..1617
                     /locus_tag="VF097_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261968.1"
                     /translation="MADRKRVSTVHLGQFSWEAANDIAGALEDAGIVWWYKQPGFLSQ
                     IWEHGIRLFVDRDRLEESKEIARRVVEELESPAES"
     gene            complement(1621..1824)
                     /locus_tag="VF097_03875"
     CDS             complement(1621..1824)
                     /locus_tag="VF097_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261969.1"
                     /translation="MEITPGLRGSRPRELPRPNRTYGEHRVCAQEGCDTRLSRYNKSK
                     HCFTHTPLTFPLVRGERKRKSAA"
     gene            complement(1948..2727)
                     /locus_tag="VF097_03880"
     CDS             complement(1948..2727)
                     /locus_tag="VF097_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6261970.1"
                     /translation="MGDLVRLEVEGGVGTILLDRPPMNAIDEDLTRDLLEVAREAARR
                     DDVGACVLYGGEKVFAAGADVKAMADMGVAEVRPYIQSLQEVFNAVEDLPKVTIAAIT
                     GFALGGGCELAMCADFRFAAENARIGQPEIQLGIIPGAGGTQRLPRLVGPARAKDLIY
                     TGRHVRGEEARAIGLVDEVHPPGEVYEAAVDAARRFAVGPRVALAAAKNAINWGARVD
                     LRTGVAFEREVFIDLFNTEDQKTGMRSFVDEGPGKATFSGR"
     gene            2799..3725
                     /locus_tag="VF097_03885"
     CDS             2799..3725
                     /locus_tag="VF097_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261971.1"
                     /translation="MGRSRAAAALLAIALSACGTAAPVGGPAGQPAVPPNADPAGPDW
                     DSSRWVAGDPFRGFGAWVDVYDEKLWADPEGTVARIADRGARTLYLQTASTSTKRRMI
                     RFPEATARFLDAARRRGVRTVAWYLPELLGAPKDLRRAVTAATFTTENGARFDGVALD
                     IEATHEPDPLLRADRLLELSRQVRGILGSDYPLGAIVPSPIRGPDFWPVLPVAELAKV
                     YDAILPMAYWTFHISGEDAAHGFISQSIRMLREEAGPEVPIHVIGGIADTSPGELRGF
                     VRAVRDADVVGASVYDVDTTDERGWRVLQELG"
BASE COUNT          517 a         1414 c         1389 g          529 t
ORIGIN      
        1 cgacgccgag atccaggacc tcctcgggga gcaggtgggg ggcttctacg acagcgatac
       61 aggcgagctc gtcgtggccg acgacgcccg ggagggcctc accacggtcg agctgctcac
      121 gctcgtgcac gagctccagc atgcgctggc ggaccagcgg ctgagcaccc cggacctcga
      181 gacgctgaac gaggaggacc aggacgcggc caccgccgcg caggcgctcg tcgagggcga
      241 cgcccagctc accaccgaga tctacgcggt gaggtcgctc agcacgacgg accagctggc
      301 ggcggcgtcc cagcaggtcc ccgggctcgg cgacacccct cacttcctgg cccgcagcct
      361 gctgttcccc tatctggagg gtgcgcagtt cgcctgccac ctgtaccggg agggcgggtg
      421 ggaggcggtg gacgccgcct acgacgacct cccgaggagc tccgccgaga tcctcttccc
      481 cgatcggtac ggctcggccc agggaccggt gaacccgacc gacccgcggg ggctcccggc
      541 gccctgggag gaggaggcga cgttcacctt cggcgcggcc gacctcctct tcctgttcga
      601 ggcccccggg ggggacgcga cgaacggcct caccgaggcc cgcgatcgcg tgcgctcgtg
      661 gaccggcggc gaggcgcacg tctgggcctg gggcggccgc accgccgtcg ggctcgtgct
      721 cgcggccagc gacggcgagg cgatgtgcga gtccgtggcg acctggtggc gccgggccgc
      781 cgaagacgcg gccgaggcca ccgaggaggt gccgggcgcg gcgctggcca cggacggccg
      841 ctcgcgcgac ggcgtgctgt actgcgagga cgggagcgtc cggctcggcg tcggccccga
      901 cctcgccgtc gcgcgagcgg ccgtccgctg acgcatgatg agcccgtgat ccggaggctc
      961 ctctgggtga tggtcctgct cgcggcgggc gccgcggccg gctacgtctg gcgcgacgcg
     1021 cggccccagg cggcggctcc acggaacgcc cctcccgtgg tcgagcagaa cgaccccgac
     1081 cccggaggtc caccggtcgt ctggctctcg ggcaccctcc gggacgtgac cgaggagcgc
     1141 ctcaccctgc aggagggcgt gggcccgacc ctcgacctga cccgcttcgc gggcggcgcc
     1201 acgcggttcc accggctggg cccgggggga tgggcccgcg tgccccgcga ggaggcggac
     1261 gcggccgccg gccaggacgc ctgcatcgag gccctcctcg accagggaga gctgctggcg
     1321 atccgggtct tcctcggggc gacctgctcc cccgccgtcc ccgggtagca tcaaggcatg
     1381 gccgaccgga aacgggtctc caccgtccac ctgggccagt tctcgtggga ggcggcgaac
     1441 gacatcgccg gcgccctcga ggacgccggc atcgtctggt ggtacaagca gcccggcttc
     1501 ctcagtcaga tctgggagca cggcatccgc ctcttcgtcg atcgggaccg cctggaggag
     1561 tcgaaggaga tcgcccggcg ggtggtggag gagctggaga gcccggccga gtcctgaggg
     1621 tcaggcggcc gacttccgct tccgctcacc ccgcaccagc ggaaacgtga gcggcgtgtg
     1681 cgtgaagcag tgcttcgact tgttgtagcg ggagagcctc gtgtcgcacc cctcctgggc
     1741 gcacacccga tgctcgccgt acgtccggtt cggacgcggc agctctcggg ggcgggaacc
     1801 gcgaagccct ggggtgatct ccatctcgct cactcctcgc tggggcgcct tgtcccccct
     1861 ggaacgcggg gagccctggg ggtattcccc ttggttcgac cgatgtcgag gggaccttta
     1921 cccggctcgg cggagcgcaa cccccggtta gcgacccgag aacgtggcct tgcccggccc
     1981 ctcgtcgacg aacgagcgca tgcccgtctt ctggtcctcg gtgttgaaga gatcgatgaa
     2041 gacctctcgc tcgaaggcga cgccggtgcg gaggtccacg cgggcccccc agttgatcgc
     2101 gttcttcgcg gcggcgaggg caacgcgcgg gccgaccgcg aaccggcggg cggcatccac
     2161 cgcggcctcg tagacctctc ccggcggatg gacctcgtcc acgaggccga tcgccctggc
     2221 ctcctcgccc cggacgtgcc gcccggtgta gataaggtcc ttcgctcgcg cgggccccac
     2281 gagccgcggc agacgctggg tcccccccgc cccggggatg atcccaagct ggatctcggg
     2341 ctgaccgatc cgagcgttct cggcggcgaa gcggaagtcg gcgcacatcg ccagctcgca
     2401 gccgcctccg agggcgaagc cggtgatcgc ggcgatcgtc accttgggga ggtcctccac
     2461 ggcgttgaag acctcctgga gcgactggat gtaggggcgg acctcggcga cgcccatgtc
     2521 ggccatcgcc ttcacgtcgg ccccggccgc gaagaccttc tcgcccccgt agaggacgca
     2581 cgcccccacg tcgtcgcgcc gggcggcctc ccgcgcgacc tcgagcaggt ctcgggtgag
     2641 gtcctcgtcg atcgcgttca tcggcggccg gtccagcagg atcgtgccca cgccgccctc
     2701 gacctcgagg cgcacaaggt ctcccatcgc tcctcctctg tcgggatcgg ccacatcgta
     2761 cgctcggagt tgacgccgtg tcgtaccgtg gacgcgtgat ggggaggtca cgcgccgcgg
     2821 cggcgctgct ggcgatcgcc ctgagcgcct gtgggaccgc ggccccggtg gggggacccg
     2881 ccgggcagcc cgccgtgccg ccgaacgcgg acccggccgg cccggactgg gactccagcc
     2941 gatgggtcgc gggggacccg ttccgcggct tcggggcatg ggtggacgtc tacgacgaga
     3001 agctctgggc ggacccggaa ggcaccgtgg cccggatcgc cgaccgcgga gcccggaccc
     3061 tgtacctcca gacggcgagc acgtccacga agcggcggat gatccgcttc cccgaggcca
     3121 ccgcccggtt cctcgacgcc gcccgccggc ggggcgtccg gacggtggcc tggtacctgc
     3181 ccgagctcct gggagccccg aaggacctgc gacgcgcggt cacggcggcc acgttcacga
     3241 ccgagaacgg cgcgcgcttc gacggcgtcg ccctcgacat cgaggcgacg cacgagccag
     3301 accctctgct gcgcgcggac cggctcctcg agctctcccg acaggtgcgg gggatcctcg
     3361 ggtcggacta cccgctcgga gccatcgtcc cctcgccgat ccgggggccg gacttctggc
     3421 ccgtcctccc cgtcgccgag ctggcgaagg tctatgacgc gatcctcccc atggcgtact
     3481 ggacgttcca catctcgggc gaggacgccg ctcacgggtt catttcccag agcatccgca
     3541 tgctgcggga ggaggccggc cccgaggtgc cgatccacgt gatcgggggc atcgcggaca
     3601 cctcccccgg cgagctccgc ggcttcgtca gggccgtgcg ggacgccgac gtggtcgggg
     3661 ccagcgtcta cgacgtcgac acgaccgacg agcgcggctg gcgcgtcctc caagagctgg
     3721 gctgagcagg gcacctgcca cgctcttgca gtagcctggg gccgtgcgac ccgaggggat
     3781 catcgagttg ctgcgggagc gcggcttccg catcacgccc cagcgcgagg ccatcatcga
     3841 ggaggtcct
//
LOCUS       DASUTZ010000089         9741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1061036, whole genome shotgun sequence.
ACCESSION   DASUTZ010000089 DASUTZ010000000
VERSION     DASUTZ010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9741
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061036"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VF097_03890"
     CDS             <1..810
                     /locus_tag="VF097_03890"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6261972.1"
                     /translation="VLVVHGITQGADGGLSDLAEELVPDGCRVLVPSRFGYLGSAMPD
                     DATSEMQADAFAEFIGALGITDAVVVAGSAGSTSALHLCVRHPARVRALVLVSANVPG
                     PHQSRGMPPRPLFKAIFGSDPILWAMITYLPGLMARLSQVLVVPEGVQLIPGDEEKLS
                     RAMRQVLLGKRRVTGEVFDAYVSNPDVNRIELSQVLAPTLVMHARDDPGPPYASAVEM
                     ADRIPDAQLVTVESGGHLMLGDHADAISEVASFILEHGSSHAEGPQGDQGR"
     gene            complement(904..1176)
                     /locus_tag="VF097_03895"
     CDS             complement(904..1176)
                     /locus_tag="VF097_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261973.1"
                     /translation="MDDEELLARAEEALTEIKVSHGLSDRHASVLAALRIRLTGSAGK
                     SLEEMLAAAGDLKPGLEEELAKTEGERKPSLDDLLSEPDGDPDRPS"
     gene            1255..2163
                     /locus_tag="VF097_03900"
     CDS             1255..2163
                     /locus_tag="VF097_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261974.1"
                     /translation="MSLRRCGVVAFLFLGMLMLAPGAVAQENGVDDDDVVILTGRLNV
                     AEGESVQNAVIFNGPAVVEGTVEEWLVVFNGRTEITGTVREDVIVFAGDVVLRSGSEV
                     QGDVITLEDPQIEDGATIGGSVDDLQTRFDLYDSTFVGRFAWWLAYTVSTLVLGLVLL
                     FLAPRLDLAAITSLRDRAGAAIGFGLMVFVLLPIVAGLLLVTVVGIPLGLFLIGGLAL
                     IYSIGYVVGGLALGRLAVREPTSRYVAFLLGWGALRVVALVPFLGGLAWFVATVLGLG
                     TLWVAGRWAPGGVRPPTPAPAPPATA"
     gene            complement(2545..3000)
                     /locus_tag="VF097_03905"
     CDS             complement(2545..3000)
                     /locus_tag="VF097_03905"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEX6261975.1"
                     /translation="MRVWTFLHILSMFLSVTASLGGDWLLLIAIRRRDLEAMRAARSV
                     WGSVEAFGFTTLFLGIAFGLIAAVVSDLDLTQGWLVAAYVLVGSGILLGLAFGPYHKR
                     LVAALDASDGDRPSPELSVLLASPRPRVATALSSLFLVLIVADMVLKPF"
     gene            complement(3000..5066)
                     /locus_tag="VF097_03910"
     CDS             complement(3000..5066)
                     /locus_tag="VF097_03910"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX6261976.1"
                     /translation="MRARTAAITGWCLAGLATGVSAISLVMVAPVSSQAVGVPQSLPF
                     GVLVVTIALTFSLVGALILSRRKDNGLGWLFAINGPVVVVSGLVRGLGYRGLFGDLGV
                     ETGRSAAWAADMSDPLVVALMTVFLYLLFPEGRLRSSGERRTALVAAVGVLLASMGAF
                     LEPHLQEYPDVSTPFALAVPPAVSWTLVGTGYAVIIGTVVSSIVLLVRRLRNARGPER
                     DQLRLLVWATVVATILIVPPIFSPSGASWTRVLYVLGGLGFLMIPVSVGVAILRHRLL
                     DIDLVIRRTVVVAVLGAFITVVYVGMVVGVGAMIGRSGNVVLSAAAAAVVALAFQPLR
                     RWAQRLASRVVYGERATPYEVLHEFSERVAGSYGTEDVLPRMAAILGEGTGASRAQVW
                     MLFGSELRPAAVWPLDARRSDAAWASGPELPSMPDVSLAVPVRDDQELLGALTVTKDA
                     SDPVTQTEERLVGDLAHQAGLVLRNVRLVEDLRASRARLVAAQDEERRKLERDIHDGA
                     QQHLVALTVKAQLADSMVDRDAEKAHELLAEIKAETTEALEALRDLARGIYPPLLADK
                     GLAAALQAQARKVTVPTEVHADGDGRYPERVETTVYFCCLEALQNTAKYADASRAIVS
                     ISASEGALTFQVSDDGRGFDPASTPRGSGLQNMRDRLEAVGGALEIRSAPGNGTTLIG
                     RLPLYG"
     gene            5268..6110
                     /locus_tag="VF097_03915"
     CDS             5268..6110
                     /locus_tag="VF097_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX6261977.1"
                     /translation="MRFKRRYRSRNVRDQRFATPARPGISGLPIGAAGGGIGGILVLL
                     LILFLNGTLGGGGAEAPEAPPRGQDPEAELVSLVSFVFDDAQSTWTDIFEESGRNYRP
                     AEMVLFRDATDTACGFASSDVGPFYCPADERVYLDLGFFEELRDRFGAEGDFAQAYVI
                     AHEVAHHVQHLLGINERVQRLSQQNPSQRNELSIQQELQADCFAGVWGKSTRERGLLQ
                     PGDVQEGLGAAAAVGDDRIQREAGGRVNPETWTHGSAEQRQTWFQRGFRAGDAEACDT
                     FAGA"
     gene            6121..6285
                     /locus_tag="VF097_03920"
     CDS             6121..6285
                     /locus_tag="VF097_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261978.1"
                     /translation="MDRARDDDLVDEASRESFPASDPPAYWAGPREERPRPRNVSVAD
                     DEDDGQGGTS"
     gene            complement(6267..7067)
                     /locus_tag="VF097_03925"
     CDS             complement(6267..7067)
                     /locus_tag="VF097_03925"
                     /inference="COORDINATES: protein motif:HMM:NF042511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6544 family protein"
                     /protein_id="HEX6261979.1"
                     /translation="MAETNVEGGGLYRRLLRELRRLGVPWAPEPGPPVTEGDLAGLPP
                     AAQRYLRFMRVVGRPRARSVRARFVGRFRLRRSWMPAEAWQYDSWPEISRVFVMRLSV
                     GGVVPIIGVDAYLGGRGWMVGKLLNFVTIVEGRGPELDTGELVTYLNDAVLLAPSFLL
                     RPDVSWREVDQHAFDVSLEDAGRSVSGRVFLDPRGAPRDFATTDRYADDPSGLVKMEW
                     RTPVSTWGMIRGRPLPGRTSAEWHRPEGPTPYIEGRFDPAAIAYDVPP"
     gene            7222..8097
                     /locus_tag="VF097_03930"
     CDS             7222..8097
                     /locus_tag="VF097_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261980.1"
                     /translation="MRTHRWLGVGLITLGAGLIVSAILGPLVLGVVDYPLSESLMNQT
                     LGLDAVSLVLVAPVSVLAGALALRGHPAGPVLGFGPAAYGWYMLLQFVVGPEYVYYPG
                     ALPLHLGLFVLSGAVAVGTWRAIRIEELPPATRRADRLRGVLLLAFSGFLLSRYVPLV
                     ADALRGEGLSPEALEGVTMFWSIFLLDLGIVLPATIAAAAGVIAGTFWGRKAMYALVG
                     WWTFVPASVAAMAIVMAANDDPNASAGQAAVFVVVAVVVLAFAAALYRGLFRADRVVS
                     APEGGAGQRHLARVG"
     gene            complement(8101..9252)
                     /locus_tag="VF097_03935"
     CDS             complement(8101..9252)
                     /locus_tag="VF097_03935"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEX6261981.1"
                     /translation="MLEVDRDQVLAYRLAAHHLTERLGPRSLVKAAVPVGVQETPTGT
                     AALAFAARVDGLTLEALDRALRVRRTLLTIWSMRGAPHVVPAADLEVFTLGALPVDAA
                     SFRQSMGGWSDALDAAELDVHETLDEMAAAARDLLDGRTMNVNELRDLVRERVPALAK
                     VRKPEFARDPMPEPMFRALGTAGAVCIVSGRGTDSEMGRTDQWLGSSPVPDSTEKARA
                     ELARRYLHAYGPSTPRHFAEWTGRSTEDAKQAFSLIEGELEEVSFGGRSWMLRRDAKA
                     LSSPSEPRGVRLLPVQDPYLQQRDRTTLLADAARRKRLWQPIRGPGGVLSGGDIVATW
                     RSTIKGRRLEVTVEPFGRLAGTVREQVAVEAELAATIRGCETVRLTFGG"
     gene            9556..>9741
                     /locus_tag="VF097_03940"
     CDS             9556..>9741
                     /locus_tag="VF097_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014362209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6261982.1"
                     /translation="MWAIAVKEFRQLRRDRRTVGLMVMAPLLLLIVFGFAARFDVDEV
                     STIVVGEGAEAARGRLPE"
BASE COUNT         1444 a         3357 c         3494 g         1446 t
ORIGIN      
        1 gttctcgtcg tccacggcat cacgcaaggt gcggacggcg gtctcagcga cctcgccgag
       61 gagctcgtcc ccgacggatg ccgagtgctc gtcccgtccc ggttcggcta cctgggctct
      121 gcgatgccgg acgacgcgac ctcggagatg caggccgatg cgttcgcgga gttcatcggc
      181 gcgctgggca tcaccgacgc agtggtggtg gcgggctcgg cggggagcac gtccgcgctc
      241 cacctctgcg tgcgccaccc ggcccgggtc cgtgcgctcg tcctcgtctc cgccaacgtt
      301 cccggtcccc accaatcgag agggatgccc ccgaggccgc tgttcaaagc gatcttcggg
      361 agcgatccga tcctttgggc catgatcacc tacctgcccg gcctcatggc acggctgagc
      421 caggtgctgg tggttccgga aggggtgcag ctgatcccgg gggacgagga gaagctctct
      481 cgagcgatgc ggcaggtcct gctcgggaag cggcgggtca ccggcgaggt cttcgacgca
      541 tacgtgtcga acccggacgt caaccgaatc gagctctcac aggtcttggc gccgacgctc
      601 gtcatgcacg cgcgcgacga ccccggtcct ccctatgcga gcgccgtaga gatggcggat
      661 cgcatcccgg atgcgcagct cgtcacggtg gagagcggtg gtcatctcat gctgggtgac
      721 cacgccgacg cgatcagcga ggtcgccagc ttcatcctcg agcacggttc gtcccacgcg
      781 gagggtcctc agggggatca aggcaggtga tccaccgagc gatgggagcg aggaggatgc
      841 cagggcgctg aaggggcagc gacagcaccc ggtgggccgt ggtcgggtcg atgggcgtcc
      901 ccctcaggat ggccggtcgg ggtccccgtc gggctcggac aggagatcgt cgagggaggg
      961 cttccgctcc ccctccgtct tcgccagctc ttcctcgagc ccgggcttga ggtcgcccgc
     1021 cgcagccagc atctcctcaa gcgacttccc ggcgctcccc gtcaggcgga tccgaagggc
     1081 ggccagcacc gaggcgtgac ggtccgacag accgtggctg accttgatct cggtgagagc
     1141 ctcctccgcc cgggccagga gctcctcgtc gtccatggac accagtctgc cacccgcgcc
     1201 ggcgacgcgc gggatgggct tgcacgtgcg cgatgggagg ttcagagtgg cggcatgagc
     1261 ctgcgccgat gcggcgtcgt ggcgtttctc ttcctcggga tgctgatgct cgcgcccggg
     1321 gccgtcgcgc aggagaacgg cgtcgacgac gacgacgtgg ttatcctcac cgggcgcctc
     1381 aacgtcgccg agggcgagtc ggtgcagaac gcggtcatct tcaacggccc agcggtagtc
     1441 gagggcacgg tcgaggaatg gctcgtcgtg ttcaacggaa ggaccgagat caccgggacc
     1501 gtgcgcgagg acgtcatcgt gttcgcgggg gatgtcgtct tgcgctcggg ctccgaggtg
     1561 cagggcgacg tcatcaccct ggaggatccc cagatcgagg acggcgccac gatcgggggc
     1621 agtgtcgacg atctccagac ccggttcgat ctctacgact cgaccttcgt cgggcgcttc
     1681 gcctggtggc tcgcctacac cgtgtcgacg ctggtgctcg gcctggtcct gctcttcctc
     1741 gccccgaggc tcgatctcgc ggccatcacg tcgctccgag accgtgcggg tgccgcgatc
     1801 gggttcggtc tcatggtgtt cgtcctgctc cccatcgtgg ccgggctgct tctcgtcacc
     1861 gtggtcggga tcccgctggg tctgttcctg atcggcggcc tcgccctcat ctactcgatc
     1921 ggctatgtcg tgggagggct cgccctgggg cggctcgcgg tcagggagcc cacctcccgc
     1981 tacgtcgcct tcctcctggg gtggggcgcc ctgcgggtgg tggcgctggt cccgttcctc
     2041 ggcggcctgg cctggttcgt ggcaacggtg ctcgggctcg ggacgctctg ggtcgccggg
     2101 cgctgggcgc cgggcggggt tcgccccccc acgccggcac ccgccccgcc cgcgacggcc
     2161 tgatcgggcc gacaccgccg actacgtcga gctcctggag aacgtccaga tccacgggtt
     2221 cgacctgagg agggaggaga ccgtcccgcc tccctcggag atcacccggc attggtacga
     2281 gacggtattc gcgcccgtga tcgaggccgc ggaaggcgaa gggtggcgcg gccgggccgg
     2341 accgagggcg acctcttcct ggagctctac cggcgacggc ggcagcgctg ggcaggccgc
     2401 ggatgccctc ctctggagga caccgtccgg aaggcgagac cccagcagtc gaggccgtgg
     2461 tgaggacgat ggcgccggct gcggcgtgac gccccgcgcc actcggctga aagggtcccg
     2521 cgcacccacg cttgtcggtc gtggtcagaa tggcttgagc accatgtcgg ccacgatcag
     2581 cacgaggaac aggctggaca gggccgtcgc caccctcggc cgaggcgatg ccaggagcac
     2641 cgagagctcg ggactcggcc ggtccccgtc gcttgcatcg agggctgcga cgagccgctt
     2701 gtggtacggg ccgaacgcca gcccgaggag gatcccggac cccacgagga cgtaggccgc
     2761 tacgagccag ccctgggtga ggtcgaggtc cgagacgacc gccgcgatca ggccgaaggc
     2821 gatccccagg aacagggtgg tgaagccgaa cgcttcgacc gagccccaca cgctgcgtgc
     2881 cgcgcgcatc gcctcgaggt cccgccgccg gatcgcgatg aggaggagcc agtcgccgcc
     2941 caacgacgcc gtgacggaga ggaacatcga gaggatgtga aggaacgtcc agacccgcat
     3001 cacccgtaaa gcggcaggcg cccgatgagc gtcgtgccgt tgcccggcgc cgaccggatc
     3061 tcgagagcac cgcccacggc ctccaagcga tccctcatgt tctggaggcc cgagcctcga
     3121 ggcgtggagg ctggatcgaa cccgcgccca tcgtctgaga cctggaacgt cagcgcgccc
     3181 tccgaggccg agatcgagac gatcgcccgc gaggcgtccg cgtacttcgc ggtgttctgc
     3241 agcgcctcga gacagcagaa gtagacggtg gtctccaccc gctccgggta gcgcccgtcg
     3301 ccgtcggcgt ggacctcggt cggcaccgtg accttccgcg cctgtgcctg cagggcggcg
     3361 gccagcccct tgtcggccag caggggaggg tagatgcccc gggcgagatc gcgcagcgcc
     3421 tccagggctt cggtcgtctc ggccttgatc tcggcgagca gctcgtgggc cttctcagcg
     3481 tcccggtcca ccatcgagtc ggccagctgg gccttcacgg tgagcgcgac gaggtgctgc
     3541 tgcgcgccgt cgtggatgtc cctctccagc ttgcgacgct cctcgtcctg cgccgccacg
     3601 agcctggccc gggacgcccg caggtcctcg accagccgca cgttccggag cacgaggccc
     3661 gcctggtgcg cgaggtcgcc gacgagccgc tcctcggtct gcgtgactgg atccgacgcg
     3721 tccttcgtca cggtcagcgc tccgaggagc tcctggtcgt cgcgaaccgg gaccgccagg
     3781 ctcacgtcgg gcatcgaggg cagctccggg cccgatgccc aagccgcatc ggaccgacga
     3841 gcgtctaatg gccacaccgc agcgggcctg agctcgcttc cgaagagcat ccagacctgg
     3901 gctcgtgacg cgcccgtccc ctcgcccagg atcgcggcca tcctcggcag gacgtcctcg
     3961 gtgccgtacg atcccgcgac gcgctcggag aactcgtgaa ggacctcgta cggggtggcc
     4021 cgttccccgt agacgacgcg gctggccagc ctctgagccc agcggcgcag cggctggaac
     4081 gcgagcgcca cgaccgcggc cgccgcggcg gacagcacga cattgccgga cctgccgatc
     4141 atcgctccca cgccgaccac catccccacg tacacgaccg tgatgaatgc gccgaggacg
     4201 gcgaccacga cggtccgccg gatgacgagg tcgatatcca gaagacgatg acgcaggatc
     4261 gccaccccga ccgagacggg gatcatgagg aacccgagac cgccgaggac gtacagcact
     4321 cgggtccagg acgctcctga cggcgagaag atcggtggaa cgatcaggat ggtggccacg
     4381 accgtcgccc agaccaggag gcggagctgg tctcgctcgg ggccccgagc gtttcggagc
     4441 ctccggacga ggagcacgat cgaggagacg accgtgccga tgatcacggc gtagccggtc
     4501 ccgacgagcg tccacgacac ggcggggggg accgcgagcg cgaagggcgt gctgacgtcc
     4561 gggtactctt ggaggtgcgg ctccaggaac gctcccatcg acgcgagcag aactccgacg
     4621 gccgccacga gggccgtccg ccgctctccg ctggaccgca ggcgtccctc cgggaacagg
     4681 aggtagagga agaccgtcat gagggcgacc accaggggat ccgacatgtc ggctgcccag
     4741 gccgcggagc ggccggtctc gacaccgagg tctccgaaga gacctcggta gccgagaccg
     4801 cggacgagcc cggagaccac gacgacgggc ccgttgatcg cgaagagcca ccccagcccg
     4861 ttgtccttcc gtcgcgagag gatcagggca ccgacgaggg agaacgtcag tgcgatagtg
     4921 accaccagga ctccgaacgg gagcgactgt gggacgccga ccgcctgtga cgagaccggc
     4981 gcgaccatca cgaggctgat cgcggaaacg cccgtcgcca accccgcgag gcaccaaccc
     5041 gtgatcgcgg ccgtccgagc cctcacgttc gttccctcct cgtcaatcgg gtcattctct
     5101 cccctgcctt ggtgccggag gaagggtgga agcaccaccg acgcggggat gtgctcacca
     5161 ccttccgccg cgcggaccga ttcgccggga tccgcgccag agcggttgcg acacgcatct
     5221 cgatgatggg ggcgtaggag tgcgagcgac gggggagaat ctctgccgtg aggttcaagc
     5281 gtcgctaccg aagccgcaac gttcgcgatc agaggttcgc gaccccggcg cgcccgggca
     5341 tctcgggtct cccgatcggc gccgccggcg ggggcatcgg gggcatcctc gtcctcctgc
     5401 tgatcctctt cctcaacggc acgctgggtg ggggaggggc ggaggcgccc gaggcgcctc
     5461 cccggggcca ggatcccgag gccgagctcg tctcgctcgt ctcgttcgtg ttcgacgatg
     5521 cgcagagcac gtggacggac atcttcgagg agtcgggaag gaactatcgg ccggccgaga
     5581 tggtgctgtt ccgggacgcg accgacacgg cttgcggctt cgccagctcc gacgtcggac
     5641 ccttctactg tcccgccgac gagcgcgtct acctggacct cggattcttc gaggagctgc
     5701 gggatcggtt cggagccgag ggggactttg cccaggccta cgtgatcgcc cacgaggtcg
     5761 cccatcacgt ccagcacctc ctcgggatca acgaacgggt ccagcgtctc tcccagcaga
     5821 acccctctca gcggaacgag ctgtcgatcc agcaggagct tcaggcggac tgcttcgccg
     5881 gggtatgggg gaagagcacg agggagcgag ggctgctgca gccgggcgac gtccaggagg
     5941 gcctgggagc cgcggcggcg gtgggggacg atcgcatcca gcgggaggcg ggcggtcggg
     6001 tgaacccgga gacgtggacc cacggttccg cggagcagcg ccagacctgg ttccagcggg
     6061 ggttccgggc cggcgacgcc gaggcctgcg atacgttcgc cggcgcgtag cctggtgggt
     6121 gtggaccggg ccagggacga cgatctcgtg gacgaggcga gcagggagtc cttccccgcc
     6181 agcgatcccc ccgcgtactg ggccggaccc cgggaggagc ggcctcgacc caggaacgtc
     6241 tccgtcgcgg atgacgaaga cgacggtcag ggcggcacgt cgtaggcgat ggcggccggg
     6301 tcgaaccgtc cctcgatgta gggcgtgggg ccctcaggcc gatgccactc ggccgaggtc
     6361 cggccgggca ggggtcgccc acgaatcatc ccccaggtcg agacgggcgt gcgccactcc
     6421 atcttgacga gcccgctcgg gtcgtccgcg taccggtcgg tcgtggcgaa gtcacgaggc
     6481 gctccgcgcg gatccaggaa gacccggccg ctcaccgacc tccccgcatc ctccagggac
     6541 acgtcgaacg cgtgctggtc cacctcccgc cacgacacgt cggggcggag gaggaacgaa
     6601 ggggccagga ggacggcgtc gttgagatac gtgacgagct cgccggtgtc gagctccggc
     6661 ccccttccct ccacgatcgt gacgaagttg aggagcttgc cgaccatcca gccccggccg
     6721 ccgaggtagg cgtccacgcc gatgatcgga acgaccccgc cgacgctgag gcgcatgacg
     6781 aacacgcggg agatctcggg ccatgagtcg tactgccacg cctcggccgg catccagctc
     6841 ctccgcagcc ggaaccggcc cacgaaccgg gcccggaccg agcgggcccg ggggcggcca
     6901 accacgcgca tgaatctcag ataacgctgg gctgccggcg ggagcccagc gaggtcgccc
     6961 tcggtcacgg gcggtccggg ctccggcgcc cagggaacgc cgaggcgtct aagctcccgg
     7021 aggagccttc gatagaggcc gccgccctcg acgttcgtct cggccacggg tcgagactac
     7081 ccggatgcgg cggtcaggct cccctcggca tcacccttcc ggaccgacga aggtcacctc
     7141 gtcctcggga cctccggccc aggagcgtcg tcggcgcgtc tcattacggt gtcggggcag
     7201 gcgatcgcag ggagaggaag gatgagaacc catcgctggc tgggggtcgg cctcatcacg
     7261 ctcggggcgg gcctcatcgt gagcgcgatc ctggggccgc tggtcctcgg ggtcgtggac
     7321 tacccgctgt ccgagtccct gatgaaccag acgctcggcc tcgacgcggt gtccctggtg
     7381 ctggtggcgc cggtcagtgt cctcgccggg gcgctggcct tgcgcggcca tcccgccggg
     7441 cccgtcctgg gcttcggtcc ggccgcctac ggctggtaca tgctcctcca gttcgtggtg
     7501 gggcccgagt acgtctacta cccgggtgcg ctcccgctcc acctggggct gttcgtcctg
     7561 agcggggctg tcgccgtggg gacgtggagg gcgatcagga tcgaggagct gccgcccgcg
     7621 accaggcgag ccgatcgcct gcgtggggtg ctcctcctgg ccttctcggg cttcctgctc
     7681 tcccggtacg tgcccctcgt ggccgacgcg ctcaggggcg aggggctgtc gcccgaagcg
     7741 ctcgaaggcg tgacgatgtt ctggtcgatc ttcctcctcg acctcgggat cgtgctcccc
     7801 gcgacgatcg ccgcggcagc gggggtcatc gccggcacgt tctgggggag gaaagcgatg
     7861 tacgcgctcg tgggctggtg gaccttcgtc ccggcctcgg tggcggcgat ggcgatcgtg
     7921 atggcggcga acgacgaccc gaatgcatcg gctggacaag ccgcggtgtt cgtggtggtc
     7981 gcggtcgtgg tcctggcctt cgcggcggcc ctttaccgcg ggctgttccg cgcggatcgg
     8041 gtcgtatcgg cgcccgaggg tggggccggc cagaggcacc tggccagggt cgggtagcgg
     8101 tcagccgccg aaggtcaacc ggaccgtctc gcagccgcgg atcgtggcgg cgagctcggc
     8161 ctcgacggcc acctgttctc ggaccgtgcc ggcgagtcgc ccgaagggct cgaccgtcac
     8221 ctcgagccgt cggcccttga tcgtcgagcg ccacgtcgcg acgatgtcgc cgcccgagag
     8281 cacgcccccc ggtccacgga tcggctgcca cagccgcttc cgacgggccg cgtccgcgag
     8341 cagcgtggtg cggtcgcgct gctgcagata ggggtcctgg acggggagca ggcggacgcc
     8401 ccgtggctcg ctcggggagg agagggcctt cgcgtcgcgg cggagcatcc aggatctccc
     8461 gccgaagctc acctcctcca gctctccctc gatcagcgag aaggcctgct tcgcgtcttc
     8521 cgtgctccgc ccggtccatt cggcgaagtg gcggggggtg ctcggtccgt aggcgtggag
     8581 gtagcgacgg gccagctcgg cccgggcctt ctccgtggag tccgggacgg gcgacgatcc
     8641 gagccactga tctgtccggc ccatctccga gtcggtgccc cggccgctca cgatgcagac
     8701 tgcccccgcc gtccccagcg cccggaacat gggctcgggc atggggtccc gggcgaactc
     8761 gggcttgcgg accttggcca gcgccggcac gcgctcccgg accaggtccc ggagctcgtt
     8821 cacgttcatg gtccggccgt cgaggaggtc gcgggccgcg gcggccatct cgtcgagggt
     8881 ctcgtggacg tcgagctccg cagcatccag ggcatcggac cacccgccca tcgactggcg
     8941 gaaggaagcg gcgtcgacgg gcagggctcc cagggtgaac acctcgagat cggccgccgg
     9001 caccacgtgc ggggcgcccc gcatgctcca gatcgtcagc agcgtgcgcc gcacccgtag
     9061 ggcccggtcc agagcttcga gcgtgaggcc atctacccgc gccgcgaagg cgagggccgc
     9121 ggtgccggtc ggcgtctcct ggacgccgac cggcaccgcg gccttgacga gggagcgagg
     9181 ccccagccgc tcagtgaggt ggtgggcggc gaggcggtag gcgaggacct gatcgcggtc
     9241 gacctcgagc accgggacga cgctacccag gccccgtgga taccgcaata gatgagttgt
     9301 ctgagcctac cggtcacatc agccggatgg agtcggaaca cgctcgcggt cgccgaggta
     9361 agcggatcgc gcctcgccgg aaccgaggaa gacgacggca tgcgccagct gttcttccgc
     9421 accaggtcga gcccgcgagt ggctggagcg gaaccatgac acggcgacgg agctgcggcc
     9481 gggcaccctc ctctccttga ccctcggaac ccagctcccg tagaattcat cgcacgttga
     9541 gttaaggggg gcgggatgtg ggcgatcgcg gtcaaagagt tccgccagct gcggcgcgat
     9601 cggcggaccg tggggctgat ggtgatggcg ccgctgctcc tgctgatcgt cttcggcttc
     9661 gcggcgaggt tcgacgtcga cgaggtctcc acgatcgtcg tgggggaggg cgccgaggcg
     9721 gcgcgggggc gcctccccga g
//
LOCUS       DASUTZ010000090        12793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_153523, whole genome shotgun sequence.
ACCESSION   DASUTZ010000090 DASUTZ010000000
VERSION     DASUTZ010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12793
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153523"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VF097_03945"
     CDS             complement(<1..390)
                     /locus_tag="VF097_03945"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEX6261983.1"
                     /translation="MKDRGDRRGGVVLAALTALVLTALPTLLAALPARASTATAARAY
                     VLSVTAASALGGLRTGLAASVLSFLALNFFFTPPFHTLTVGRVEDVVALAVFLIVSGI
                     VGTLLSRAVSQRARAERREREARLLHRV"
     gene            516..701
                     /locus_tag="VF097_03950"
     CDS             516..701
                     /locus_tag="VF097_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261984.1"
                     /translation="MVASLGEIAEFLAGLAVGVLIGVVLAPLVGFWVRRHEWKEASRK
                     ARLTEDVLDLMDSMARR"
     gene            698..3019
                     /locus_tag="VF097_03955"
     CDS             698..3019
                     /locus_tag="VF097_03955"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX6261985.1"
                     /translation="MSTPVLKRVLMGRALASHALEHQRLPKTLALPIFSSDPLSSNAY
                     ATEEMMLVLALAGTVAFRLMVPIALLIVLVLAIVITSYRQTVRAYPLGGGSYIVAHEN
                     LGPIPGVVAASALLSDYVLTVAVSITAGTLAITSAAPGLLPYKVPLALGLIALVAVAN
                     LRGVKEAGMLFAVPAYGFVITVYVLLITGFLRCTDGCPTAETADLPIHPEHALTFFLV
                     LRAFASGSTALTGVEAIADGVQAFRRPQSRNAATTLAVMGAITIGMFMGITVLARAIE
                     LRVTEELAARKSVLAQIGETVFGGDDPMFYVLQAFTALILILAANTAYQDFPRLSAIL
                     ATDHYMPRQFTNRGDRLVFSNGIVVLSLLAGLLVWIFDANLTRLIQLYLVGVFVAFTL
                     SQAGMVRRWLRLREKGWARGAVINGIGAAATGVVLVIIVATKFMHGAWIVVLAIPLVV
                     LLLLSIFRHYERVRVTLRSRGLTTDAEVENTFVVLVPDLGLATREAISWLLTVRPDPG
                     RVIPLYLGPDPLEVAAAEWARLAPRLGELRRLEVENGQEVRSLRRFVRGIERGPDDFV
                     TVVVPERVSTGLLLYVLRRTRSFRVKAAFLLEPGVVVTDVPLLPEELEVAAGRALEER
                     PLELARNVCIVPVSAVHDATIRALAYARSLRPAHLEAIFLAGEPHEADEILADWREKR
                     IDVPLSAIEAPFRDYGPPFLEEIRRHTKRGDTIVTVVLPEFVVDRWRHALLHNQRALF
                     FKRTLLREPWVVAVSVPYQIGPARRAARASSRA"
     gene            complement(3016..3531)
                     /locus_tag="VF097_03960"
     CDS             complement(3016..3531)
                     /locus_tag="VF097_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015659032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261986.1"
                     /translation="MATMARVRAGPAETRLRVGSWLMGLAAAGFIAYAVVFLILSFTS
                     RFLELGIGPEQVDVGRDEIRAFSPSLSHYIDHLHIAVAGFIAAIGAAVLYLVMFGVRR
                     GALWAWVGAVAATVLALGISLPAHYPNGFDTLAHLGPVYVATAIFAAGAVLALRGILA
                     ERRAQGNPERH"
     gene            complement(3545..3715)
                     /locus_tag="VF097_03965"
     CDS             complement(3545..3715)
                     /locus_tag="VF097_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261987.1"
                     /translation="MPRHYRIMISIQDEDDKEIDSSVHEEDYEDDDEARRRFQEKTEA
                     ARGAGRGRPQRQ"
     gene            complement(3775..6927)
                     /locus_tag="VF097_03970"
     CDS             complement(3775..6927)
                     /locus_tag="VF097_03970"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6261988.1"
                     /translation="MKACATCGTPNPEEARFCQACGSPMVQAPAEWEQRKTVTVVFAD
                     VTGSTALGEALDPESLRRLMARFFMEMSAVLQRHGGSVEKFIGDAVLAVFGRPTVHED
                     DALRAVRAAVEMRQVLRSLNEELQRAHGVTLQIRTGINTGTVVIGDLVAGQSFVTGDA
                     INVAARLEQLASPGEILIGEDTFRLVRHAVTAEPAPPLKVRNREEPVVAYRLEDVHDA
                     APRGESPLRSPMVGRVRDLGVLLDLVDEVTKRDEARLATILGPAGVGKSRLVDELVAG
                     LGEQLPSLSGRCLTYGEGITYWPVSEVVRQALGIRGESSPAEIRRLLAERVRGSDHAD
                     RIVEGVAQMMGLSDAAGGQEQMFWGTRRFLEVVAGEKPLLVIFDDVHWGEPTFLDFVE
                     YLADFASAPLLLVCMARLDLLEARPGWGTGRRNATTLTLDPLTEGESVALIRNLLRVE
                     DLDPAASRHLIRATEGNPLFIEEMLRMLIDEGLVKQEGGGWASVGDVTSLPAPPTIQA
                     LLSARLARLRPEELAAIERASVVGKVFSWTSVAALTPEEERSEVGGHLQALVRKELIL
                     PDTEVMDGEDTFRFRHLMIRDAAYEAIPKEVRADLHELLASWLEARGGEEYDEILGHH
                     LEQAYRWRIEIAPVDERARALAERAVEQFASAGRRAFARGDVPAAVRLLPHAVALLPG
                     DHPMRPRLLADLGRALMEHGELERADEVLTEAARSAAEATARLSRLLVRLSTHPEDTT
                     DALRAEVDEVAPVFEELRDERGLANVARLRAEIEWLASRFGAAAEHLERTIEHARRAG
                     DRRLEMDALAQLAAAILFGPTPADEGIRRCREIYERSGGDRRVEAAVLAVEAELRAMQ
                     GRFEGTREQIAEGQAILEDLGLRFRSRASEALASVEMLAGDPEAARRAYMSGYETLEE
                     IGERGFLSTTAAELALVAWAQEDYEDAERFVRISEESGASGDVATIVPAKRVRAKLLA
                     RQGSFEEAERIARQAVDAARRTDDLNLQADALLDLAEVLRTADREHEGIEPLEEAAAL
                     YEEKGNLVGAQRARALLRSA"
     gene            6974..7056
                     /locus_tag="VF097_03975"
     tRNA            6974..7056
                     /locus_tag="VF097_03975"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7007..7009,aa:Leu,seq:taa)
     gene            7111..8715
                     /locus_tag="VF097_03980"
     CDS             7111..8715
                     /locus_tag="VF097_03980"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6261989.1"
                     /translation="MRRSVTVGILLALPMAFLALLLLRPELDVAWEHHPSHFWLVLGV
                     ALVSVALGLATNSAARQRGDARVFLVSLSFLSSAAFLGLHALATPDVLLSAPNTGFVI
                     ATPIGLLLASGFAATSALPLEVAGIAWVRRWEGVLRWGLAALVAVWAVASLTRLPFLD
                     RPFPEEAPGVLRGIALAGVALYAFAAIRYWWVFRRRPSVLLLATAVAFVFLAEAMVTV
                     ALSRSWRISWWEWHALMAVAFLVISLAARREYRRQGSVTGAFGGLYLHATLQRLESRH
                     AEALAELAHRLDAGEPLAPVMDRLGGEGFTAEELTLLERSARELSRVDQLFSPYVGPA
                     LAERLRREPDLATLGGTEREVTVLFADLMGFTSFSEGRSPVEVIEMLNSYWAVAVPLL
                     AEREGGLIERFAGDSVLVVFNALGDHPDHALRAARAALELRDETEKIWAAHAGWPRFR
                     IGLNTGPVVVGNVGAGEQRSFSVVGDTPNVAARIQSLAEPGHVLAGPETTARIRGQVE
                     LVSRGERALKGKSEPVELFELIRLAA"
     gene            9030..9311
                     /locus_tag="VF097_03985"
     CDS             9030..9311
                     /locus_tag="VF097_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010474066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX6261990.1"
                     /translation="MIKRFRDRDAERLFNRRPTRRLGPGVQKVALRKLRMLDAAARLD
                     DLRVPPGNCLERLRGDRTGQHSIRINDQWRICFRWRAGDAYDVEIVDYH"
     gene            9318..9617
                     /locus_tag="VF097_03990"
     CDS             9318..9617
                     /locus_tag="VF097_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEX6261991.1"
                     /translation="MMTKRLPPVHPGDILLEEFLSPMGISQYRLAKDIGVPPRRINEI
                     VHGTRAVSADTALRLARYFRTSERFWLNLQAQYDLDVEYDRSGDRIEREISPRTV"
     gene            9815..10228
                     /locus_tag="VF097_03995"
     CDS             9815..10228
                     /locus_tag="VF097_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261992.1"
                     /translation="MSEQSPFPRSIYGRAFGEGFVAALVDSRVEATRLVELLAVTGIR
                     RGDVGVLPGELALVIDASHHPSLAVSPPAPGEVAVSEKYVAGALLGDVLLAVRTPAER
                     RARVVAGILLSEGASLVFHFGPGFLRAPDLTEAAA"
     gene            10225..>12793
                     /gene="nifJ"
                     /locus_tag="VF097_04000"
     CDS             10225..>12793
                     /gene="nifJ"
                     /locus_tag="VF097_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEX6261993.1"
                     /translation="MTRPVVTLDGNEAAAYVAHKASEVIAIYPITPASPMGELADAWS
                     ARGDPNVWGSVPEVIEMQSEAGAAGAVHGALQAGALTTTFTASQGLLLMLPNMYKIAG
                     ELTPAVFHIAARTVATHALSIFGDHSDVMGARSTGWAMLASSSVQEAQDLALVAHAAT
                     LESRVPFLHFFDGFRTSHELMKIERVTEDDILAMLSEEAIDEHRARRLTPDRPVIRGT
                     AQNPDVFFQAREAAGPFHLAVPEIVDGVMDRFHELTGRRYRPFEYVGHPEAERVLVLM
                     GSGVGAAEEAVERLVAEGERVGLVKVRLFRPFSIERFAEALPESVRAIAALDRTKEPG
                     AIGEPLYQDVVTALVERRGRPVPVVIGGRYGLSSKEFTPAMAAAALDELDRPEPKPHF
                     TVGITDDVLHTSLKVDHGFSTEPDDVARAVFYGLGSDGTVSASKSSVKIIGEHTPLHA
                     QGYFVYDSKKAGSVTVSHLRFGPRPIRSTYLIDRATFVACHQTHFLERMDVLEVADDG
                     ATFLLNTPYGPDEVWERLPIEVQEQIIAKGLRFFVVDGHRVAREAGLAGRINTVLQTC
                     FFALSGILPRDEAVAAIKEGIEKAYGKRGREVLERNFAAVDASLDALREVAVPDAIEG
                     ERHRLPPVPDEAPDFVRRVTGAIIARQGDLLPVSAFPVDGTFPSGTARWEKRSIALEL
                     PAWDPEICIDCAKCVLVCPHAAIRMKVWDPEVVEAPEAVQHKAWRGKDFAGHRLTIQV
                     APDDCTGCAVCADICPAVSKEDPSHKSLTMVPTADRLEADRAGWEVFQELPTVDRTEV
                     RPDTVKGSGLLEPLFEFSGACAGCGETPYLGLLSRLFGDRTLVANATGCSSIFGGNLP
                     "
BASE COUNT         1764 a         4518 c         4506 g         2005 t
ORIGIN      
        1 gacgcgatgg aggagacggg cctcccgctc gcgacgctcc gctcgggccc tctgcgagac
       61 ggctcgggag aggagggtcc cgacgatccc cgagacgatc aggaagacgg cgagcgcgac
      121 cacgtcctcg accctcccca ccgtcagggt gtggaagggc ggggtgaaga agaagttcag
      181 cgcgaggaag gagagcacgg acgccgcgag ccccgtgcgg aggcccccca gcgccgacgc
      241 cgcggtcacc gagaggacgt aggccctcgc ggcggtggcg gtggacgccc tggcggggag
      301 ggcggcgagc agggtcggga gggccgtgag cacgagcgcc gtcagggcgg cgaggaccac
      361 gccccctctc cggtctccgc gatccttcat cccgaacact ctggccttgc cctgagggcc
      421 ccacaaccga ccgggcgagt ccatacgcct tctttacgcc tcgactcctc gtcctgacgg
      481 cgtcctgacg gctgcggcgt gaccatcgct acaccatggt ggcatcgctc ggcgagatcg
      541 cggagttcct cgccggcctg gcggtcggcg tgctcatcgg cgtcgtcctg gcccccctgg
      601 tcggcttctg ggtgaggcgg cacgaatgga aggaagcctc caggaaggcg cgtctcaccg
      661 aggacgtgct cgatctgatg gactcgatgg cccgccgatg agcacgccgg tcctgaagcg
      721 tgtcctcatg ggtcgcgccc tcgccagcca cgcgctggag catcagcgcc tgcccaagac
      781 actggccctg cccatcttct cctccgaccc gctctcctcg aacgcctacg ccaccgagga
      841 gatgatgctc gtcctggctc tcgccgggac ggtggcgttc cggctcatgg ttcccatcgc
      901 tctcctcatc gtgttggtcc tcgccatcgt gatcacgtcg taccgacaga ccgtgcgcgc
      961 gtaccctctc ggcgggggct cctacatcgt ggcgcacgag aacctcggcc cgatccccgg
     1021 cgtggtcgca gcctccgcgc tcctgtccga ctacgtcctg accgtggccg tcagcatcac
     1081 cgcaggcacc ctggcgatca cgtccgcggc cccgggactg ctgccctaca aggtgccgct
     1141 ggccctggga ctcatcgcgc tcgtggcggt ggccaatctc cggggcgtga aggaggcggg
     1201 catgctcttc gccgttcccg cgtacgggtt cgtgatcacg gtctacgtgc tgctgatcac
     1261 cggcttcctc cggtgcaccg acggatgccc gacggcggag accgcggatc ttccgatcca
     1321 tccggagcac gcgctcacgt tcttcctcgt cctccgagcg ttcgcatcgg gctccaccgc
     1381 cctcaccggc gtggaggcca tcgccgatgg cgtgcaggcg ttccggcgcc cgcagtcgag
     1441 gaacgccgcc accacgctgg ccgtgatggg tgcgatcacc atcgggatgt tcatgggcat
     1501 caccgtgctg gcgagggcga tcgagctgcg ggtcacggag gagctggccg cgaggaagtc
     1561 ggtcctcgcg cagatcgggg agacggtgtt cggcggggac gacccgatgt tctacgtgct
     1621 ccaggcgttc acggcgctga tcctcatcct cgcggccaac acggcgtacc aggacttccc
     1681 ccggctctcg gcgatcctgg ccaccgacca ctacatgccc aggcagttca cgaaccgcgg
     1741 cgacaggctc gtgttctcga acggcatcgt cgtgctctcg ctcctggcgg ggctcctcgt
     1801 ctggatcttc gacgcgaacc tcaccaggct gatccagctc tacctggtcg gggtgttcgt
     1861 ggccttcacc ctgtcccagg cggggatggt gcgccggtgg ctgaggcttc gcgagaaggg
     1921 atgggcgcgg ggcgccgtca tcaacggcat cggggcggcg gcgacgggcg tcgtgctcgt
     1981 gatcatcgtg gcgacgaagt tcatgcacgg cgcgtggatc gtggtgctgg cgatccctct
     2041 ggtcgtcctc ctcctcctct cgatcttccg ccactacgag cgcgtccgcg tcacgctgcg
     2101 ctccaggggt ctcacgacgg acgctgaggt ggagaacacg ttcgtcgtcc tggtgcccga
     2161 tctggggctc gccaccaggg aggcgatctc gtggctgctc accgtgcgcc cggatcccgg
     2221 tcgagtcatc cccctctacc tgggccccga tcccctggag gtcgccgcgg ccgagtgggc
     2281 caggctggcc ccccgcctcg gggagctgcg ccgcctggag gtggagaacg gtcaggaggt
     2341 ccggtccctc cgccggttcg ttcggggcat cgagcgcggc cccgacgact tcgtcacggt
     2401 ggtcgtcccc gagagggtct cgacggggct gctcctctac gtcctgcgca ggacccgatc
     2461 cttccgggtg aaggccgcct tcctgctgga accgggcgtg gtggtcacgg acgtgcccct
     2521 cctccctgag gagctggagg tcgccgccgg cagagcgctg gaggagcgcc ccctggagct
     2581 ggcccgcaac gtgtgcatcg tcccggtgtc ggcggtccac gacgcaacga tccgggcgct
     2641 cgcctacgct cgctcgctcc gccccgctca cctggaggcg atcttcctgg ccggggagcc
     2701 gcacgaggcc gacgagatcc tggcggattg gagggagaag cggatcgacg tcccgctgag
     2761 cgcgatcgag gcgccgttcc gtgactacgg gccgcccttc ctggaggaga tccgccggca
     2821 cacgaagcgc ggagacacca tcgtcacggt ggtgctgccc gagttcgttg tagaccggtg
     2881 gcgccacgcc ctcctccaca accagcgcgc cctgttcttc aagcgcacgc tgctgaggga
     2941 gccgtgggtg gtcgccgtca gcgtgcccta ccagatcggc ccggctcgcc gcgccgcaag
     3001 ggcttcctcg cgcgcctaat gcctttcggg attcccctga gctcggcgct ccgccaggat
     3061 cccgcgcagc gccagcaccg cgcccgcggc gaagatcgcc gtcgccacgt atacgggccc
     3121 gaggtgcgcg agggtgtcga agccgttggg gtagtgggcg ggaagcgaga tccccagcgc
     3181 gaggaccgtc gccgcgaccg ccccgaccca ggcccagagg gctcctcgcc gcaccccgaa
     3241 catcacgagg tacagcacgg cggccccgat cgccgcgatg aagccggcca ccgcgatgtg
     3301 caggtggtcg atgtagtggg agagcgaggg gctgaacgcc cggatctcgt cgcggcccac
     3361 gtcgacctgc tccgggccga tcccgagctc gaggaaccgg ctcgtgaagc tcaggatcag
     3421 aaagacgacg gcgtacgcga tgaacccggc ggcggccagg cccatcagcc aggatccaac
     3481 cctgagccgg gtctccgcgg gcccagctcg tacccgggcc atcgtggcca tgtacgagtc
     3541 cggcctactg gcgctgtggg cggcctctcc cggccccgcg ggcggcttcc gtcttctcct
     3601 ggaacctgcg gcgagcctcg tcgtcgtcct cgtagtcctc ctcgtggacc gacgagtcga
     3661 tctccttgtc gtcctcgtcc tggatcgaga tcatgatccg gtagtgcctc ggcatacgcc
     3721 ccctcccggt ctcgggctcc attctgttcc ggtggcgctc gggggtgcaa ccgctcaggc
     3781 ggagcggaga agcgctcggg cccgctgggc gccgacgagg ttgcccttct cctcgtacag
     3841 ggcggccgcc tcctccagcg gctcgatccc ctcgtgctcg cggtcggccg tcctgaggac
     3901 ctcggccagg tcgagcaggg cgtcggcctg caggttgagg tcgtcggtcc ggcgggcggc
     3961 gtccacggcc tggcgagcga tgcgctcggc ctcttcgaag ctgccctgtc gggcgagcag
     4021 cttcgcccga acccgcttgg cggggacgat cgtggccaca tcgccggagg cgccgctctc
     4081 ctcgctgatc cgcacgaacc gctcagcgtc ctcgtagtcc tcctgcgccc aggccaccag
     4141 ggccagctcc gcggcggtcg tggagaggaa gccgcgctcg ccgatctcct cgagggtctc
     4201 gtagccgctc atgtaggcgc ggcgggcggc ctccgggtcg ccggccagca tctcgacgga
     4261 ggccagcgcc tcggaggcgc gggagcggaa gcggaggccg aggtcctcga ggatggcctg
     4321 gccctcggcg atctgctccc gggtgccctc gaaccggccc tgcatggccc ggagctccgc
     4381 ctcgaccgcg aggacggcgg cctcgacgcg ccggtcgccg ccggagcgct cgtagatctc
     4441 gcggcagcgc cggatgccct cgtcggcggg ggtggggccg aacaggatcg ccgcggcgag
     4501 ctgggccagg gcgtccatct ccaggcgtcg gtcgccggcc cggcgggcgt gctcgatcgt
     4561 ccgctccagg tgctccgcgg ccgcgccgaa gcgcgaggcg agccactcga tctcagcgcg
     4621 gagacgggcc acgttggcca ggccgcgctc gtcgcggagc tcctcgaaga ccggcgcgac
     4681 ctcgtccacc tcggcgcgaa gggcgtcggt ggtgtcctcg gggtgggtgg agaggcggac
     4741 caggaggcgg gagaggcggg cggtggcttc ggccgccgac cgcgcggcct ccgtcagcac
     4801 ctcgtccgcc cgctccagct ccccgtgctc catcagcgcc ctccccaggt ccgccaggag
     4861 ccggggccgc atcgggtgat cgccggggag gagcgccacg gcgtgcggca ggaggcggac
     4921 agcggccggg acgtcccccc gggcgaacgc ccgtcgaccc gccgacgcga actgctccac
     4981 ggcccgctcg gcgagcgccc gcgctcgctc gtcgaccggc gcgatctcga tcctccatcg
     5041 gtaggcctgc tccaggtggt gcccgaggat ctcgtcgtac tcctcgcccc ctcgcgcctc
     5101 cagccaggag gccagcagct cgtggaggtc ggcccggacc tccttcggga tcgcctcata
     5161 ggccgcgtcc cggatcatga ggtgtctgaa ccggaaggtg tcctccccgt ccatcacctc
     5221 ggtgtccggg aggatcagct ccttgcgcac aagcgcctgg aggtgtcccc cgacctccga
     5281 ccgttcctcc tccggcgtca gcgccgccac cgacgtccac gagaagacct tccccacgac
     5341 gctggcccgc tcgatcgccg cgagctcctc gggccggagc cgcgccaggc gggccgagag
     5401 cagcgcctgg atcgtgggcg gggccgggag gctggtgacg tcccccacgc tcgcccaccc
     5461 tccgccctcc tgcttcacga gcccctcgtc gatcagcatc cggagcatct cctcgatgaa
     5521 cagcgggttg ccctccgtgg cccggatcag gtggcggctc gcggcgggat cgaggtcctc
     5581 gacgcggagg aggttccgga tcagcgcgac gctctcgccc tcggtcagtg ggtcgagcgt
     5641 cagggtggtg gcgttgcgcc ggcccgtgcc ccagcctggc ctcgcctcca ggaggtcgag
     5701 ccgggccatg cagaccagga gcagtggggc cgaggcgaag tccgccaggt actccacgaa
     5761 gtcgaggaag gtgggctcgc cccagtggac gtcgtcgaag atcacgagga ggggtttctc
     5821 gcccgcgacg acttcgagga accggcgcgt gccccagaac atctgctcct ggccgccggc
     5881 tgcgtcgctg aggcccatca tctgggccac gccctccacg atccggtcgg cgtggtcgct
     5941 ccctcggacc cgctcggcga ggagcctccg gatctcggcg ggcgacgact cccctcggat
     6001 gccgagggcc tgccgcacga cctcgctgac gggccagtac gtgatcccct cgccgtaggt
     6061 gaggcagcgc cccgagagcg agggcagctg ctccccgagc ccggcgacga gctcgtccac
     6121 gaggcgcgac ttccccacgc ccgcaggccc caggatcgtg gccaggcggg cctcgtcccg
     6181 cttcgtcacc tcgtccacga gatcgaggag gacgcccagg tccctgacgc gcccgaccat
     6241 gggggagcgg aggggcgact cgccgcgagg ggccgcgtcg tgcacgtcct cgaggcggta
     6301 ggcgaccacc ggctcctcgc ggttccggac cttcagcggc ggggcaggct cggcggtgac
     6361 ggcgtgccgg acgagccgga aggtgtcttc accgatcagg atctcgccgg gggaggccag
     6421 ctgctcgagt cgggccgcga cgttgatcgc gtcaccggtg acgaaggact gcccggccac
     6481 gaggtctccg atgacgaccg tgcccgtgtt gatgccggtc ctgatctgca gggtcacccc
     6541 gtgcgcccgc tgcagctcct cgttgagcga gcgaaggacc tgccgcatct cgaccgcggc
     6601 ccggaccgct cgcagagcgt cgtcctcgtg cacggtgggc cgcccgaaca ccgccagcac
     6661 ggcgtcgccg atgaacttct ccacgctgcc cccgtgacgc tggaggacgg cgctcatctc
     6721 catgaagaag cgcgccatca accggcgcag cgactccggg tcgagggcct ctcccagcgc
     6781 ggtggacccc gtgacgtcgg cgaacacgac cgtgacggtc ttccgctgct cccactccgc
     6841 gggggcctgg accatgggcg acccgcacgc ctgacagaag cgggcctcct cggggttcgg
     6901 ggtgccgcac gtcgcgcacg ccttcacgaa aggcagtcta cggccgcggc ccgcgcgccg
     6961 gtatccttgg agcgcccggg tggcggaacg gtagacgcag ctcccttaaa aggagaaggc
     7021 cgaaaggccg tgtgggttcg actcccaccc cgggcactcg acacgggcgt gcggcccggc
     7081 ggcccatctc gggataggct cgcctccgtg atgcggcgct cggtcaccgt cgggatcctc
     7141 ctggccctgc ctatggcctt cctggccctc ctcctcctcc ggccggagct cgacgtcgcc
     7201 tgggagcacc atccctccca cttctggctc gtactgggcg tcgccctggt gagcgtagcg
     7261 ctgggcctgg cgacgaactc cgcggcacgg cagcgaggcg atgccagggt ctttctggtc
     7321 tccctcagtt tcctgtcgag cgcggccttc cttggcctgc acgcgctggc aacccccgac
     7381 gtcctgctgt ctgctcccaa caccggattc gtgatcgcca cgccgatagg gctgctcttg
     7441 gcgtcgggct tcgccgctac gtcggcgttg ccgctggagg ttgcggggat cgcgtgggtc
     7501 cggcggtggg agggcgtcct ccggtggggg ctggcggccc tggtggcggt ctgggccgtc
     7561 gcgtctctga cacggcttcc gttcctcgac cggccgttcc ccgaggaagc gcccggcgtc
     7621 cttcgaggga tcgcgctggc cggagtggcg ctttacgcgt tcgccgcgat ccggtactgg
     7681 tgggtgttcc ggcggcggcc gtcggtcctc ttgctggcca ccgccgtcgc cttcgtgttc
     7741 cttgcagagg ccatggtcac ggtggcgctg agccgcagct ggcgcatctc atggtgggaa
     7801 tggcacgcgc tgatggcggt ggcgttcttg gtcatcagct tggcggcccg gcgcgagtac
     7861 cgccgccaag gatccgttac gggggcgttc ggaggcctct acctgcatgc gacgctgcag
     7921 cgcctcgaga gcaggcacgc ggaggcgctg gcggagttgg cgcacagact ggacgcagga
     7981 gagccgctgg cgccggtgat ggaccgactg gggggcgagg ggttcaccgc cgaggagctc
     8041 acgctgctgg agcggtccgc tcgcgagctg agccgcgtcg atcagctctt cagtccctac
     8101 gtgggccccg ccctcgccga gcggctccgc agggagcccg atctcgccac gcttggtgga
     8161 acggaacgcg aggtcaccgt gctgttcgcc gacctcatgg gcttcacgtc cttctccgaa
     8221 ggccgcagcc ccgtcgaggt gatcgagatg ctcaattcct actgggcggt ggccgtgcca
     8281 ctgctcgccg agcgggaggg cggcctcatc gagcggttcg ccggcgattc ggtgctcgtg
     8341 gtgttcaacg cactgggcga ccatcccgat cacgcactgc gtgcggcgcg ggcggctctc
     8401 gagctccgcg acgagaccga gaagatatgg gccgcccacg cggggtggcc gcgattccgg
     8461 atcggcctca acaccggccc ggtcgtggtg ggaaacgtcg gcgccggcga acagcgcagc
     8521 ttctcggtgg tcggggacac gcccaacgtg gcggccagga tccagtcgct ggcggagccg
     8581 gggcacgtcc tggccggccc ggagaccact gcccggatac gaggccaggt ggagctcgtc
     8641 agccgaggcg agcgggctct aaagggcaag tccgagcccg tcgagctctt cgagttgatc
     8701 cggctcgccg cctgaggggt ttgacccaat ttagggatca ccagggcggg tcgcaaggat
     8761 gttcaggttc tgtgcaggcc ttcagcggcc gggcggcccc ggcgtccgcg gccatgggac
     8821 gcgaactaca agctcaagaa cctggctggg gaatgtgggt tctgtgggcg acgggccccg
     8881 ggcacgcacg ggtttccact cggcttctcg gcctcgggct gggcaggagg ccgaggcgcc
     8941 gtccaggctg ccgggtatca gttccgaagc ttcggtgacc tgaagctcca agatctagac
     9001 gctcttaacg cgtcgcgtta tcattggttg tgatcaagag gttccgcgac cgagacgccg
     9061 agcgattgtt caatcggcgg ccgacgcggc gattgggacc gggggtgcaa aaggtggcct
     9121 tgcgaaagct tcggatgctg gatgctgcgg cgaggctcga tgatctccgt gtgccgccgg
     9181 gaaactgttt ggagcggctg cgcggcgatc gcactggtca gcacagcatc cggatcaacg
     9241 accagtggcg gatctgcttc cggtggcgcg ctggcgatgc atacgacgtt gagatcgtcg
     9301 actaccacta gggggcgatg atgacaaaga gacttccacc cgttcatccc ggtgacatcc
     9361 tcctcgagga gttcctgtcg cccatgggaa tcagccagta ccgcctcgcc aaggacatcg
     9421 gcgtcccccc tcgcaggatc aacgagatcg tccacggaac gcgggcggtg tccgccgaca
     9481 ccgctttgcg gctggctcga tacttcagga cctctgagcg attttggctg aacctccagg
     9541 cgcagtacga cttggatgtg gaatacgacc gcagtgggga ccggatcgag cgggagatct
     9601 cccctcggac cgtatgatca ccgttcccgt gtggtgggcc cctccgggga cctgacgaac
     9661 accagctcag agatcccgat caactcggcc tcatgcgtct tgtggactca gctcccttcg
     9721 ggcgaaggtc ccggatgcat ggggtcctcc ggccctgcga cgtgatcctc cggtcctgca
     9781 tgctcgctcg gaagatgcgt ggaggaggtc acggatgagc gagcagagcc cgttccccag
     9841 gtcgatctac ggacgggcgt tcggcgaggg gttcgtcgcc gcgctggtgg atagccgggt
     9901 cgaggcgacg cgtctggtgg agctgctggc ggtcaccggc atccggcggg gcgacgtggg
     9961 ggtccttccg ggggagctgg cgctcgtgat cgacgcgtcg caccatccgt cgctggccgt
    10021 ctccccgccc gctccgggcg aggtcgccgt ctccgagaag tacgtggccg gcgcgctgct
    10081 gggagacgtg ctcctcgccg tccgaacccc cgccgagcga cgagctcggg tcgtggccgg
    10141 gatcctcctc tcggagggag cctccttggt cttccacttc ggaccggggt tcctccgagc
    10201 tcccgacctg accgaggccg cggcgtgacc cgcccggtgg tcaccctcga cggcaacgag
    10261 gccgccgcct acgtcgccca caaggccagc gaggtcatcg cgatctaccc gatcaccccc
    10321 gcctccccga tgggagagct ggccgacgcg tggtcggccc gcggggaccc gaacgtctgg
    10381 ggaagcgtcc ccgaggtcat cgagatgcag agcgaggccg gggcggccgg cgccgtgcac
    10441 ggagccctgc aggcgggggc cctcacgacc accttcacgg catcccaggg gctcctgctg
    10501 atgctgccga acatgtacaa gatcgcgggc gagctcacgc cggcggtctt ccacatcgcc
    10561 gcccgcaccg tcgccaccca cgccctctcg atcttcgggg accacagcga cgtgatgggc
    10621 gctcgctcca cgggatgggc catgctggcc tcgtcctcgg tccaggaggc ccaggacctg
    10681 gcgctggtgg ctcacgccgc gacgctcgag tcgcgcgtgc cgttcctcca cttcttcgac
    10741 gggttccgca cgtcgcacga gctgatgaag atcgagcggg tcaccgagga cgacatcctg
    10801 gccatgctga gcgaggaagc gatcgacgag caccgcgccc gccgcctcac ccccgaccgc
    10861 ccggtgatcc ggggcacggc ccagaacccg gacgtgttct tccaggcgag ggaggcggcc
    10921 ggccccttcc acctcgccgt cccggagatc gtggacggcg tgatggaccg cttccacgag
    10981 ctgaccgggc gccgctaccg gccgttcgag tacgtggggc accccgaggc cgagcgggtg
    11041 ctggtcctga tggggtcggg cgtgggcgcg gccgaggagg ccgtggagcg cctggtcgcg
    11101 gagggcgagc gggtgggtct ggtcaaggtg cgcctgttcc gcccgttctc gatcgagcgg
    11161 ttcgccgagg ccctgcccga gtcggtgcgg gccatcgccg cgctcgaccg gacgaaggag
    11221 ccgggggcga tcggcgagcc tctgtaccag gacgtcgtga ccgcgctcgt cgagcgccgc
    11281 ggccgcccgg tgccggtcgt gatcggggga cggtacggcc tgtcctccaa ggagttcacg
    11341 cccgccatgg cggccgcggc gctcgacgag ctcgaccggc ccgagcccaa gccgcacttc
    11401 accgtgggga tcaccgacga cgtcctccac acgagcctca aggtggatca cgggttctcc
    11461 accgagcccg acgacgtggc ccgggccgtc ttctacgggc tggggagcga cggcacggtc
    11521 agcgccagca agtcctcggt gaagatcatc ggcgagcaca cgccgctcca cgcgcagggc
    11581 tacttcgtct acgactcgaa gaaggcgggc tcggtgacgg tctcgcacct gcggttcggg
    11641 ccgcggccca tccgctccac ctacctgatc gaccgggcca cgttcgtggc ctgccaccaa
    11701 acccacttcc tggagcggat ggacgtgctc gaggtggccg acgacggcgc aaccttcctc
    11761 ctcaacaccc cctacggccc ggacgaggtc tgggagcgcc tcccgatcga ggtgcaggag
    11821 cagatcatcg ccaagggcct ccggttcttc gtggtggacg gccaccgggt cgcgcgcgag
    11881 gcggggctgg ccgggcggat caacaccgtc ctccagacct gcttcttcgc gctgtcgggg
    11941 atcctgccgc gggacgaggc cgtggcggcg atcaaggagg ggatcgagaa ggcctacggg
    12001 aagcggggcc gggaggtcct ggagcggaac ttcgccgccg tggacgcgtc gctggacgcc
    12061 ctccgagagg tcgcggtccc cgatgcgatc gagggcgagc gccatcggct gccgccggtc
    12121 cccgacgagg cgccggactt cgtccgtcgg gtcaccggag cgatcatcgc ccggcagggc
    12181 gacctcctgc ccgtcagcgc cttccccgtg gacggcacgt tcccctcggg cacggcgaga
    12241 tgggagaagc gcagcatcgc tctggagctg cccgcctggg acccggagat ctgcatcgac
    12301 tgcgccaagt gcgtcctcgt gtgcccccac gccgcgatcc ggatgaaggt ctgggacccc
    12361 gaggtcgtgg aggctcccga ggcggtccag cacaaggcgt ggcgggggaa ggacttcgcg
    12421 ggccatcggc tgacgatcca ggtcgctccc gacgactgca ccgggtgcgc ggtctgcgcg
    12481 gacatctgcc cggcggtcag caaggaggat ccatcccaca agtccctgac gatggtcccc
    12541 acggccgacc ggctggaggc ggatcgagct gggtgggagg tgttccagga gctgccgacc
    12601 gtggaccgga ccgaggtgcg acccgacacg gtgaagggct cggggctcct ggagccgctg
    12661 ttcgagttct ccggcgcctg cgccggctgc ggggagaccc cgtatctcgg cctgctgagc
    12721 aggctcttcg gcgaccgaac gctggtggcc aacgcgaccg ggtgctcctc gatcttcggg
    12781 gggaacctcc cca
//
LOCUS       DASUTZ010000091        11180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_543890, whole genome shotgun sequence.
ACCESSION   DASUTZ010000091 DASUTZ010000000
VERSION     DASUTZ010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11180
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543890"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VF097_04005"
     CDS             <1..225
                     /locus_tag="VF097_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HEX6261994.1"
                     /translation="IEVEDDTAALAHEATVSKVGEDQLFYLMSRGLSEQEATAMVVNG
                     FIEPITRELPMEYAVELNRLIELQMEGAVG"
     gene            327..1376
                     /locus_tag="VF097_04010"
     CDS             327..1376
                     /locus_tag="VF097_04010"
                     /inference="COORDINATES: protein motif:HMM:NF021446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HEX6261995.1"
                     /translation="MMLGVGVLAILFLVILIVAARRSHDTIPAAAGGPSIPERLSDEL
                     DRWVGAGLITRADAEALARFEARRVGGTRRGLVVEVVGYLGAILAVAGAGVAVGQAWD
                     DLQTWQRVIVPFATAAALLVAGLVISRSEEPALGRMMSVLWLMSVASLAWGVGVAGVD
                     VLDVWSQRMPLLVGGVSLAYAGALWWFRRWPAQQLATFVALEVTVVGAIIAPWGSEPR
                     WAPMLGAWAVGALWLGLGWRRLLTPGWMAPLLGALALVWAPTLGAGLDWNVLFGLATA
                     AGLMALSVPTRDVALLGIGSFGAFGYLVATAVTYFGESLGVPMTLFFSGLAILGVAVV
                     GGRLFRLTRRPSEQT"
     gene            complement(1348..2301)
                     /locus_tag="VF097_04015"
     CDS             complement(1348..2301)
                     /locus_tag="VF097_04015"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX6261996.1"
                     /translation="MGIARSALLWASTNRILRRMAPRVWFVRRALRRFMPGETLEDGL
                     EAAETFRRLGIPTLFTHLGENVAGRREAIHATEDYLRAIDRVEALGLDTEVSVKLTHL
                     GLDLDPGLAAENYLRLVRRAAERGDTAWIDMESSEYVERTLEVYRTTLEASPNAGLCL
                     QAYLHRTRADLEDLLPLGPSIRLVKGAYREDPELALQRPDEIDEAFLGLSVTLLRGLQ
                     EGKVRRYAAGSHDLRLLGRVATASRDLGVSVKDWEIEMLYGIRHADQLELTRRGYPVR
                     TLISYGPAWYPWYVRRLAEKPGENLAYVLRNVFTSAPTASG"
     gene            2383..2532
                     /locus_tag="VF097_04020"
     CDS             2383..2532
                     /locus_tag="VF097_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEX6261997.1"
                     /translation="MAAIDPVCGMTVEESEAAATFEHEGRTYYFCSVDCREEFEENPE
                     DYAEA"
     gene            2533..2886
                     /locus_tag="VF097_04025"
     CDS             2533..2886
                     /locus_tag="VF097_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX6261998.1"
                     /translation="MAALGAALVVFLFWTGSFLVLRWAGDALNPPVATAPSRVPFLGG
                     RGPETHAWSRFHVRYYAMALVFLAFDMEMVFMYPWAVVFLEEGLMAGIEMLMFIAILV
                     LGILYGWREGAFRWA"
     gene            2877..4166
                     /locus_tag="VF097_04030"
     CDS             2877..4166
                     /locus_tag="VF097_04030"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6261999.1"
                     /translation="MGLRSALARFASGAPTPVFVVGGVGGRRAAQHLQLSSVLAPTPS
                     PRAADALVVAGFLTERLLPAVMLAHDQIPAPRLAFQWLPDGAPDDPLAAALPGLRVVE
                     GGALPSIIRTEHERLLLGGGVRDDAWPDVEPATWRDIGPYRQGGKGMTGGVPFGRPLA
                     GRADDRDGLKLDRLDVRVGPLFPAFPPGLELVLGVQGDVAQEVAVGANPFEGITLGAR
                     TEDVFARVAREAVPIAELEGARARSHLRWLADALRVAGLPSLGRRVLALAVRAGSGDV
                     RRVARLRRSLERGRGLWWLARGVGVVREDVGGPAARAAGHPSDLRMELDEYRALGFEP
                     VVGEGGDALARWRQRVAEAEQSLRLAGAAGDAAIGPLDVVEGPRGPIGAPGVGAGALL
                     DLLPGLVVGMEWGDLVTTVVSLDLDLEEAAALEEVPA"
     gene            4163..5092
                     /locus_tag="VF097_04035"
     CDS             4163..5092
                     /locus_tag="VF097_04035"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019019750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEX6262000.1"
                     /translation="MRIAASVLATVAAMALGVYVAAALDALAAAALGGRSRLVRSLTF
                     PARRGLALLLRPRARTERPDAEAWALAPAALAGLAAVGLATIPLAPGLAVADADAGIV
                     LFGAAMALVMVAVFLQGWGPNSLHPLIAGYRFAGQALSYEMPLALVLIGAALPAQSLS
                     VGAIVESQAHLWNVIRQPLGMPLFLVAVAGLAFWGPLALPEGEDLAGGTSAEVSGAAR
                     LTWKAARLMVLVAGAAMTAAVFLGGWHGPWVPGWAWTILKTLAVLVLLVWARHRFVRV
                     PVERFVWVSWVVLIPLALLDVFLAGAQALWAAP"
     gene            5089..5640
                     /locus_tag="VF097_04040"
     CDS             5089..5640
                     /locus_tag="VF097_04040"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007068915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX6262001.1"
                     /translation="MIVDVLSWIFGVGSVLCAWRVFRADSMVRAAYWLLAAFVFVGLL
                     LVLLTARFLGLVLILMMAGEMTIMAVFMVMFMMNPAGLNPMMMVHQHRTSIAAGVVAF
                     LGLAAVGLLGSFPERAVAAGARVTRETGMELLGDSMVVFETAAVALLATMIGAVALAS
                     RRGRAGDAYEGSVPPTMDPEEAP"
     gene            5637..5942
                     /gene="nuoK"
                     /locus_tag="VF097_04045"
     CDS             5637..5942
                     /gene="nuoK"
                     /locus_tag="VF097_04045"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006911983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX6262002.1"
                     /translation="MSQELLLVVAAALFGIGVYGALSQQAFVMIMMGLELMIAGGILA
                     IGTFWAQSAAGSPKGQVLAIVAMTVMAVEAALGFALVIAVYRVRKADMTEKLKRLKG"
     gene            5942..7843
                     /locus_tag="VF097_04050"
     CDS             5942..7843
                     /locus_tag="VF097_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX6262003.1"
                     /translation="MLWLAPLAPLAVAGLAAAARAGWAPWVAGGGVVVSLVGSLMGAA
                     GEWTASWGWGPALGLSLSADGIARVVAVLVPAVALPVLAYAASDHRGDPGRRRLLSLL
                     ALFVGAMQMVVLAADLLTLLIGWELVAACSWALIGHRWREDAPRAGQEAFVVTRVGAV
                     GLVVAAGAALHATGSLRFEDLGGATAVDLHLIAAGVLAAALAKSAQPPFSPWLFSAMA
                     GPTSVSALLHSATMVAAGAYALARLAPALEPTGWFGPAVIGFGLAGALLGGIVALLQS
                     DVKRALAASTTAQYGLVLVAVGALAVPATAGHLAAHAVAKASLFLAAGVAIHAAGSGD
                     LTRMRLGRALPAIAWLFAVGAASLAGVPPLGGAFTKDAVIGAAWEHATWAGVGALLAG
                     ALSALYAGRIALLAYGPGEARRPAAPPALQRAAIAVLTLLVVLLGLLWLPPVREGLAG
                     IVGGDAVVPAAWEILLSVGAIVLAAVIVLLLDRRRALASLGLPDRLRFPAADWLGLPA
                     AGRVLVVTPVLALSRGLARLDDSVVDAGVRAVGRVADLFARVFRAWGERGVDGLVEGV
                     GRGTLASADGSRRVDEGLVDGSVEGVARGVGAAGAASRLLQSGLAHRYYVIIAVGLAV
                     AAAVAFLVR"
     gene            7851..9329
                     /locus_tag="VF097_04055"
     CDS             7851..9329
                     /locus_tag="VF097_04055"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX6262004.1"
                     /translation="MLTAAIFAPLAAAIAIALLPTARERLLRGLALVAAAFPLVPLAV
                     TWGRMEGGEIQLVEAVDWIPTLGVGYRVGVDGVSLTLAALTALLFLVSLIFPEDVRGR
                     PKAYFALFLFLETASLGVFLAMDLFLFYVFWDLSLAGMYFVIGGWGHGDRQRSALKFF
                     LYTLAGSLAMLLAILGLYLATDPRTFDMIEIVRQQPVAGTGLRASLIFLGFAIGFAIK
                     APLVPFHTWLPPAHVDAPAPGSTILAGVLLKMGTYGFIRILMPMLPGAFRAYALALAI
                     LGVVSIVYGALVTLAQRNVKRLIAYSSVTHMGYTMLGIAAAGALATGEVEARSLALTG
                     ATVEMVAHGLITGGLFLIAGTLWRRGETYDLDALGGLASRATRATGVTVVLAFASLGL
                     PGLAGFVAEFQIFVGTFSVYPWLAGIGLLGVIVLAAAFLRMLREAFLGPLPEELRDWT
                     DLDAAETVSLAPLLLLTVVIGIAPAWLLDVIDRTARALLGGG"
     gene            9329..10741
                     /locus_tag="VF097_04060"
     CDS             9329..10741
                     /locus_tag="VF097_04060"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX6262005.1"
                     /translation="MPVGSITPELIVLGGSVGVLLWALFAPRRLQAPGAAILSLAVIA
                     AAGIATWPMLRGGQGTTFFGTYAVDDTAVWAKLLILGATAVTVLLSFEWFRTEPRHGE
                     YYSLLLMSALGAILLAGAADLMEVLLGVLLASATGYVLAGYHRAHAPSGEAAIKYFLL
                     GAFANAAMVFGVALLFGLGGTTLLGGLRAGLVSASTVGLVAGAVLVLVGLLFKMGAVP
                     AHAWMPDVAEGSPAPVAAFVTVAPKIGALIAVGRLMLALPEDGVGWRPLIAVVAALTM
                     TLGNLTALWQEDVRRLLGWSAVSQSGYGLMAVVALGRSDLAVPSLLFFLAAYALANLA
                     AFGVVIELRGRTDRVRYAGLGRTHPALALSLVVALLSFVGIPPLAGFTAKLALFGATI
                     EAGYTWLAVLAILNTVMSLAYYARVLAPAYFEPGPEPLPVLGRWTRTATLAAAAGVMV
                     VGILAQPFLDGFSLARLFPG"
     gene            10766..>11180
                     /locus_tag="VF097_04065"
     CDS             10766..>11180
                     /locus_tag="VF097_04065"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6262006.1"
                     /translation="MTDQPPGAEERGVALPEGTVTVLFTDVEGSTDLRTREGDDVAQE
                     LLRAHEHLLREQVEAHGGREVVFMGDGFMIAFGSTRSALRCAVAIQRTFDEHNRAHPD
                     RPIRVRAGLNAGEVRQEGGTLYGTAVNAAARIMGKA"
BASE COUNT         1142 a         3808 c         4373 g         1857 t
ORIGIN      
        1 atcgaggtcg aggacgacac ggccgcgctg gcccacgagg ccacggtctc caaggtcggg
       61 gaggaccagc tcttctacct gatgagccgg gggctctccg agcaggaggc caccgcgatg
      121 gtcgtcaacg gcttcatcga gccgatcaca cgggagctcc cgatggagta cgcggtcgag
      181 ctgaaccgac tgatcgagct ccagatggag ggcgcggtcg gctgaggccg gccggcgcag
      241 ctcgccgaag gtcccccgtt cgggccgtcg ctcggccctg accatgcgtc cgcgcattct
      301 gcatcctgag ggtggaggtg agggcgatga tgcttggggt cggagtcctc gcgatcctgt
      361 ttctcgtcat cctgatcgtc gcggcgaggc gctcgcacga cacgatcccg gccgcggcag
      421 ggggcccgag catccccgag cggctctcgg acgagctcga tcgatgggtc ggcgcgggcc
      481 tcatcacgcg ggccgacgcg gaggcgctcg ctcgcttcga ggcccgccgg gtgggtggga
      541 cccgtcgagg gctcgtggtg gaggtcgtcg ggtacctcgg cgcgatcctg gccgtggcgg
      601 gcgcgggcgt cgcggtgggg caggcctggg atgacctgca gacgtggcag cgggtaatcg
      661 tgcccttcgc cacggcggcg gcgctgctgg tcgccggcct cgtgatctcc cgatccgagg
      721 agcccgcctt gggccgcatg atgagcgtcc tgtggctgat gtcggtggcc tctctggcct
      781 ggggggtggg cgtcgccggc gtggacgtgc tggacgtgtg gagtcagcgc atgccgctcc
      841 tcgtgggagg cgtctcgctc gcgtacgcgg gcgccctgtg gtggttccgc cggtggccgg
      901 cacagcagct ggcgaccttc gtcgccctgg aggtcaccgt agtcggagcg atcatcgcgc
      961 cgtggggcag cgagccgagg tgggccccca tgctcggtgc gtgggccgtg ggagccttgt
     1021 ggctggggct cggttggcgc cggctcctga cgcccgggtg gatggcaccc ctgctgggcg
     1081 ctctggcgct ggtctgggcc ccgacgctcg gcgccggcct cgactggaac gtgctgttcg
     1141 gtttggccac ggccgcgggg ctgatggccc tgagcgtgcc gacgcgggac gtcgcgctcc
     1201 tgggcatcgg ctccttcggg gcgttcggct acctggtggc cacggccgtg acgtacttcg
     1261 gggagagcct cggcgtgccg atgacgctgt tcttctcggg gctggcgatc ctgggcgtgg
     1321 ccgtggtggg gggtcgtctg ttccgcctca cccggaggcc gtcggagcag acgtgaagac
     1381 gttgcgcagg acgtaggcga ggttctcgcc gggcttctcg gcgagccgcc ggacgtacca
     1441 cgggtaccag gcgggcccgt aagagatcag cgtgcgcacg gggtagccgc ggcgggtcag
     1501 ctcgagctgg tcggcgtggc ggatcccgta gagcatctcg atctcccagt ccttcaccga
     1561 cacgccgaga tcgcgcgagg cggtggcgac gcggccgagc aggcggaggt cgtggctccc
     1621 ggcggcgtag cgacgaacct ttccctcctg gaggccccgc agcagcgtga ccgagagccc
     1681 gaggaacgcc tcgtcgatct cgtcgggccg ctgcagggcg agctcgggat cctcgcggta
     1741 ggcgcccttc accagccgga tcgaggggcc cagggggagg aggtcctcga gatcggcccg
     1801 cgtgcggtgg aggtaggcct ggagacagag cccggcgttc ggggatgcct ccagggtcgt
     1861 gcggtagacc tcgagcgtcc gctcgacgta ttcgctcgac tccatgtcga tccaggcggt
     1921 gtcgccccgc tcggcggcgc gtcgaacgag ccgcaggtag ttctctgctg cgaggcccgg
     1981 gtcgagatcg aggccgaggt gcgtgagctt caccgacacc tcggtatcga gtcccagcgc
     2041 ttcgacccgg tcgatcgccc ggaggtagtc ctccgtggcg tggatcgcct cccgccggcc
     2101 ggccacgttc tccccgaggt gggtgaagag agtggggatc ccgagccggc ggaaggtctc
     2161 ggcggcctcc aggccgtcct cgagcgtctc gcccggcatg aaccgacgca gggcccgccg
     2221 cacgaaccac acgcggggcg ccatccggcg caggatccgg ttcgtggacg cccacagcag
     2281 cgcgctcctg gcgatgccca tcgccgggag ggtagccgga acgacggtcc cgtgacgacc
     2341 ccgggtatcg tgtgcgcgag agaacgaccg aaggagaccc ggatggcggc gatcgacccc
     2401 gtgtgcggga tgacggtgga ggagagcgag gcggcggcca cgttcgagca cgagggcagg
     2461 acctactact tttgcagcgt ggactgcagg gaggagttcg aggagaaccc cgaggactac
     2521 gcggaggcct agatggccgc gctcggcgcc gccctcgtcg tcttcctctt ctggacgggg
     2581 agcttcctcg tcctgcggtg ggccggggac gccctgaacc cgccggtcgc gacggcgccg
     2641 tcgcgcgtgc cgttcctggg gggccggggc ccggagaccc acgcctggag ccgcttccac
     2701 gtccgctact acgcgatggc gctcgtcttc ctcgccttcg acatggagat ggtcttcatg
     2761 tacccgtggg ccgtcgtctt cctggaggag ggcctgatgg cggggatcga gatgctgatg
     2821 ttcatcgcga tcctcgtgct ggggatcctg tacggctggc gcgagggggc cttccggtgg
     2881 gcctgaggtc ggcgctggct cgcttcgcct ccggggcgcc cacgccggtg ttcgtggtcg
     2941 gcggggtcgg ggggcggcgc gccgcccagc acctccaact gtcgtcggtc ctggcgccga
     3001 cgccgtcgcc ccgggcggcc gacgcgctcg tcgttgccgg gttcctgacc gagcgccttc
     3061 tgcctgctgt gatgctcgcg catgaccaga tcccggcacc ccggctcgcg ttccagtggc
     3121 ttccggacgg cgcgccggac gatccgctgg cggcggcact gcccgggctg cgggtcgtgg
     3181 agggcggcgc gctgccgtcg atcatccgca ccgagcatga gcgcctcctt ctaggtgggg
     3241 gcgtccggga tgatgcctgg cccgacgtgg agccggcgac ctggagggac atcgggccgt
     3301 accgccaggg cgggaagggc atgaccggcg gcgtgccgtt cggacgcccg ctggcggggc
     3361 gggccgacga ccgcgacggg ctgaagctcg atcgcctcga cgtgcgggtg gggccgctgt
     3421 tcccggcgtt ccctcccggg ctggagctcg tcctgggcgt gcagggcgac gtggcccagg
     3481 aggtggccgt cggagcgaac ccgttcgagg gaatcacgct cggcgcccgc accgaggacg
     3541 tcttcgcgcg cgtcgcgcga gaggcggtcc cgatcgcgga gctggagggc gcgcgggccc
     3601 ggagccacct ccggtggctg gcggacgcgt tgcgggtagc gggactcccg tcgcttggac
     3661 gtcgggtcct cgccctggcg gtccgggcgg gatccgggga cgtccggcgg gtcgcgcggc
     3721 tgcgccgctc gctggagcgg ggccgcggcc tctggtggct cgcccgcggg gtcggggtgg
     3781 tgcgcgagga cgtcggaggg ccggcggctc gcgcggcggg tcatccgagc gacctccgga
     3841 tggagctgga cgagtaccgg gcgctggggt tcgagccggt ggtcggggag ggcggcgacg
     3901 cgctggcgcg gtggcgccag cgcgtcgccg aggccgagca gtcgctcagg ctggcggggg
     3961 ccgccgggga tgccgcgatc ggacccctcg acgtcgtgga ggggccccgc gggccgatcg
     4021 gagcgcccgg ggtcggggcc ggcgcgctcc tcgatctcct gccgggcctc gtggtgggca
     4081 tggagtgggg cgatctcgtg acgacggtgg tgagcctcga cctcgacctg gaggaggccg
     4141 cggcgctgga agaggttccg gcatgaggat cgccgcctcg gtgctggcga cggtggcggc
     4201 gatggcgctc ggtgtctacg tcgcggcggc gctggatgcc ctggccgccg cggcgctcgg
     4261 cggccgctcc aggctggtgc ggtcgctgac gttccccgcg cggcgggggc tcgcgctcct
     4321 gctccgcccc cgcgccagga ccgagcgccc cgacgccgag gcctgggcgc tcgcccccgc
     4381 cgccctcgcc ggcctcgccg ccgtcgggct ggccacgatc cccctggcgc ccggtctggc
     4441 cgtggccgac gccgacgcgg gcatcgtcct gttcggggcc gcgatggctc ttgtgatggt
     4501 ggcggtgttc ctccagggat ggggcccgaa ctccctccat cccctgatcg cgggctaccg
     4561 cttcgcaggg caggcgctct cgtacgagat gcccctggcg ctggtgctga tcggagccgc
     4621 cctgccggcc cagtcgctct cggtcggcgc gatcgtcgag tcccaggccc acctgtggaa
     4681 cgtgatccgc cagccgctgg ggatgccgct gttcctcgtg gcggtggcgg ggctggcgtt
     4741 ctggggcccg ctggccctgc ccgaggggga ggatctcgcc ggcggcacct ccgccgaggt
     4801 ctccggcgcc gcgcgtctga cctggaaggc ggcccggctc atggtgctcg tggcgggggc
     4861 ggcgatgacg gccgccgtct tcctcggggg atggcacggt ccgtgggtcc ccgggtgggc
     4921 ctggacgatc ctcaagacgc tcgcggtgct ggtcctgctg gtgtgggccc ggcaccgctt
     4981 cgtgcgcgtg ccggtcgagc ggttcgtgtg ggtctcgtgg gtcgtgctga tcccgctggc
     5041 gctcctggac gtcttcctgg ccggggctca ggccctgtgg gcggcgccgt gatcgtcgac
     5101 gtcctgtcct ggatcttcgg cgtcggctcg gtgctgtgcg cgtggcgcgt gttccgggcc
     5161 gactcgatgg tgcgggccgc ctactggctc ctggccgcgt tcgtcttcgt cgggctcctg
     5221 ctcgtcctgc tgacggcccg gttcctggga ctcgtcctga tcctgatgat ggccggcgag
     5281 atgacgatca tggccgtctt catggtcatg ttcatgatga acccggccgg gctgaacccc
     5341 atgatgatgg tccatcagca tcggacgtcg atcgcggcgg gagtcgtggc gttccttggg
     5401 ctcgccgcgg tcgggctcct gggctcgttc ccggagcggg cggtggcggc gggagcccgc
     5461 gtgacccgcg agacggggat ggagctcctc ggggactcga tggtggtctt cgagacggcc
     5521 gccgtggccc tgctggccac gatgatcggg gcagtggcgc tggcctcgcg gcgggggagg
     5581 gccggcgacg cgtacgaggg gtctgtacct cccacgatgg acccggagga agcgccgtga
     5641 gccaggagct cttgctcgtc gtggcggccg cgctgttcgg gatcggcgtc tatggggcgc
     5701 tgagccagca ggcgttcgtg atgatcatga tgggcctcga gctgatgatc gcggggggca
     5761 tcctcgcgat cggcacgttc tgggcgcaga gcgctgcggg ctcacccaag ggacaggtcc
     5821 tggcgatcgt cgccatgacc gtgatggcgg tggaggcggc gctggggttc gcgctggtca
     5881 tcgccgtgta ccgcgtgcgg aaggccgaca tgaccgagaa gctgaagagg ctgaagggct
     5941 gatgctctgg ctcgcccccc tcgcgccgct cgccgtcgcg ggcctggccg cggcggcccg
     6001 ggccggatgg gctccctggg tcgccggcgg cggggtggtg gtctccctcg tcgggtcgct
     6061 gatgggcgcg gcgggggagt ggacggcatc gtgggggtgg gggcccgcgc tcggcctctc
     6121 gctgtcggcc gacgggatcg cccgcgtcgt ggccgtcctg gtcccggccg tggcgctgcc
     6181 cgtgctggcc tatgcggcgt cggaccatcg gggggatccg gggcggcgtc gtctcctctc
     6241 gctcctggcc ctgttcgtgg gggcgatgca gatggtcgtg ctcgcggccg acctcctcac
     6301 gctgctgatc gggtgggagc tcgtggccgc gtgctcctgg gccctgatcg ggcaccggtg
     6361 gcgcgaggac gcgcccaggg cgggccagga ggccttcgtc gtgacccggg tgggggccgt
     6421 ggggctcgtg gtcgcggcgg gggccgcgct ccacgccacg ggctccctgc gcttcgagga
     6481 tctgggcgga gccaccgccg tggacctgca cctgatcgcg gcgggcgtcc tggccgcggc
     6541 cctggcgaag tcggcccagc cgccgttctc gccgtggctg ttctcggcga tggccggccc
     6601 gacctcggtc tcggcgctgc tgcactccgc gacgatggtg gcggcgggcg cctacgccct
     6661 ggcccgcctg gctccggcgc tggagccgac cggatggttc gggcccgcgg tgatcggttt
     6721 cgggctggct ggtgcgctgc tggggggcat cgtggcgctg ctccagtcgg acgtgaagcg
     6781 ggcgctggcc gcctccacga cggcgcagta cgggctcgtg ctggtcgcgg tgggggcgct
     6841 cgccgtgccc gcgaccgccg ggcacttggc ggcgcacgcc gtggccaagg cgtcgctgtt
     6901 cctggcggcc ggcgtggcca tccacgccgc gggctcgggc gatctcacga ggatgcgcct
     6961 cggccgggcc ttgcccgcga tcgcctggct gttcgcggtg ggggcggcct cgctggccgg
     7021 cgttcccccg ctgggtggcg cgttcacgaa ggacgccgtg atcggggcgg cgtgggagca
     7081 cgcgacgtgg gcgggggtgg gggcgctgct ggccggcgcg ctctccgcgc tgtacgcggg
     7141 gaggatcgcc ctgctcgcct acggcccggg ggaggcgcgc cggccagcag cgccccccgc
     7201 gctccagcgg gccgcgatcg ccgtcctgac cctgctcgtc gtgctcctcg gcctcctctg
     7261 gctcccgccc gtcagggagg gcctggccgg gatcgtgggg ggcgacgccg tcgtcccggc
     7321 ggcgtgggag atcctgctct cggtgggagc gatcgtgctc gcggcggtga tcgtcctcct
     7381 cctcgaccgg cgacgcgcgc tcgcgtcgct cgggctgccg gaccggctgc ggttcccggc
     7441 cgccgactgg ctcggtctcc ccgccgccgg tcgagtcttg gtggtgacgc ccgtcctggc
     7501 tctctcccgg gggctggccc ggctcgacga cagcgtcgtg gacgccgggg tccgggcggt
     7561 gggccgggtc gccgacctct tcgcccgcgt cttccgggcg tggggagagc ggggcgtgga
     7621 cggcctggtg gagggcgtcg ggagggggac gctggcgtcg gccgacggct cccggcgggt
     7681 ggacgagggc ctcgtggacg gctcggtcga gggggtggcg cgtggcgtgg gggcggccgg
     7741 ggccgcgagc cggctcctcc agtccggcct cgcccaccgc tactacgtga tcatcgccgt
     7801 ggggctcgcg gtggcggccg ccgtcgcctt cctggtgaga tgagcggagc atgctgaccg
     7861 ccgccatctt cgcgccgctg gctgccgcga tcgctatcgc gctcctgccg acggcccgcg
     7921 aacggctcct gcgcggcttg gccctggtcg ccgcagcctt cccgctcgtc cccctggccg
     7981 tcacctgggg ccgcatggag ggcggcgaga tccagctcgt ggaggccgtc gactggatcc
     8041 ccacgctggg tgtcggctac cgggtggggg tggacggcgt gtcgctcacg ctggccgccc
     8101 tcacggcgct gctgttcctc gtctcgctga tcttccccga ggacgtccgc ggaaggccca
     8161 aggcctactt cgcgctcttc ctcttcctgg agacggcttc gctcggtgtc ttcctggcga
     8221 tggacctgtt cctcttctac gtgttctggg acctgagcct ggccgggatg tacttcgtga
     8281 tcgggggatg gggccacggc gaccggcagc gctcggcgct gaagttcttc ctctacacgc
     8341 ttgccggctc gctggcgatg cttctggcga tcctggggct gtacctggcc accgatcccc
     8401 gcacgttcga catgatcgag atcgttcgtc agcagcccgt ggccgggacg gggctccggg
     8461 cctcactgat cttcctcggg ttcgcgatcg ggttcgcgat caaggcgccg ctcgtgccgt
     8521 tccacacgtg gctgccgccc gcgcacgtcg acgcgcccgc tcccggctcg acgatcctgg
     8581 ccggcgtcct cctgaagatg gggacctacg ggttcatccg gatcctgatg ccgatgctcc
     8641 cgggggcctt ccgcgcgtac gcgctggcgc tggcgatcct cggcgtggtc tcgatcgtgt
     8701 acggggcgct cgtcaccctg gcgcagagga acgtgaagcg gctgatcgcg tactcgagcg
     8761 tgacgcacat ggggtacacg atgctgggga tcgcggcggc gggagcgctc gccaccggcg
     8821 aggtcgaggc gcggtcgctc gcgctgaccg gcgccacggt ggagatggtg gcccacgggc
     8881 tcatcacggg agggctcttc ctgatcgcgg gaacgttgtg gcggcggggc gagacttacg
     8941 acctcgacgc gctgggcggg ctcgcgtcgc gcgcgacgcg ggccacgggc gtcaccgtcg
     9001 tgctggcctt cgccagcttg gggctcccgg gcctggccgg cttcgtggcc gagttccaga
     9061 tcttcgtggg gaccttctcc gtgtacccgt ggctggccgg gatcggcctc ctcggggtga
     9121 tcgtgctggc ggccgcgttc ctgcggatgc tccgcgaggc gttcctgggg cccctccccg
     9181 aggagctccg cgactggacc gacctcgatg ctgccgagac cgtctcgctg gccccgctcc
     9241 tgctcctcac cgtggtgatc gggatcgctc ccgcctggct cctcgatgtg atcgaccgaa
     9301 ccgcccgcgc actgctcgga ggcggctagt gccggtcggg tcgatcaccc ccgagctgat
     9361 cgtcctcggc gggtcggtcg gcgtgctgct ctgggcgctc ttcgcgcccc ggcgcctgca
     9421 ggcccccggg gccgcgatcc tctcgctcgc cgtgatcgcg gccgcgggga tcgcgacgtg
     9481 gccgatgctc cgcgggggac agggaacgac gttcttcggg acctacgcgg tggacgacac
     9541 cgccgtctgg gccaagctgc tgatcctcgg ggcgaccgcg gtcacggtcc tgctctcgtt
     9601 cgagtggttc cggacggagc ctcgccacgg cgagtactac agcctgctcc tgatgtcggc
     9661 gctgggcgcg atcctgctgg cgggcgcagc cgatctgatg gaggtcctgc tcggggtcct
     9721 gctcgcctcg gccaccgggt acgtcctggc cggctaccac cgggcgcacg cgccctcggg
     9781 cgaggcggcg atcaagtact tcctcctcgg agcgttcgcc aacgccgcca tggtgttcgg
     9841 cgtggccctg ctgttcgggc tcggcgggac gacgctgctc ggggggcttc gggccgggct
     9901 ggtctcggcc tcgacggtcg ggctcgtggc gggcgcggtg ctggtcctgg taggcctgct
     9961 gttcaagatg ggagcggtgc cggcccacgc ctggatgccg gacgtggccg agggctcgcc
    10021 ggcgccggtg gccgcgttcg tcaccgtggc ccccaagatc ggggccctga tcgcggtggg
    10081 tcgcctgatg ctcgccctcc ccgaggacgg cgtggggtgg cggcccctga tcgccgtcgt
    10141 ggccgctctc accatgaccc tggggaacct caccgcccta tggcaggagg acgtgcggcg
    10201 cctgctgggg tggtcggccg tctcgcagtc gggctacggg ctgatggcgg tggtggcgct
    10261 ggggaggagc gatctcgcgg tgccctccct gctcttcttc ctcgccgcct acgcgctggc
    10321 gaacctggcc gccttcggcg tggtgatcga gcttcggggc cgcaccgacc gcgtccggta
    10381 cgcgggcctg ggtcggaccc acccggccct ggccctctcg ctcgtggtgg cgctcctctc
    10441 tttcgtgggg atcccgccgt tggccggctt caccgcgaag ctggcgctct tcggggcgac
    10501 gatcgaggca gggtacacgt ggctggcggt gctggcgatc ctcaacacgg tgatgtcgct
    10561 cgcctactac gcgcgggtgc tggcgcccgc ctacttcgag ccgggccccg agccgctccc
    10621 cgtgctgggg cggtggactc gcaccgccac gctggccgcc gcggccggcg tcatggtcgt
    10681 ggggatcctg gcccagccct tcctcgacgg cttctcgctg gcccgtctgt ttccgggctg
    10741 aacgccatcg cgataccctg cggtcgtgac cgaccagcca cccggagcag aggagcgcgg
    10801 tgtcgcgctc cccgagggca ccgtcacggt gctgttcacc gacgtcgagg gatccacgga
    10861 tctccgcacc cgcgagggcg acgacgtcgc ccaggagctg ctccgggcgc acgagcatct
    10921 tctccgcgag caggtcgagg cgcacggcgg ccgcgaggtc gtcttcatgg gggacgggtt
    10981 catgatcgcg ttcggctcga cgcggagcgc cctgcggtgc gccgtggcca tccagagaac
    11041 gttcgacgag cacaaccgcg cccaccccga ccggccgatc agggtgcggg cgggcttgaa
    11101 cgccggtgag gtgcggcagg agggcgggac gctctacggg accgccgtga acgcggccgc
    11161 acggatcatg gggaaagccg
//
LOCUS       DASUTZ010000092         4256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_153660, whole genome shotgun sequence.
ACCESSION   DASUTZ010000092 DASUTZ010000000
VERSION     DASUTZ010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153660"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VF097_04070"
     CDS             complement(<1..579)
                     /locus_tag="VF097_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020498885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX6262007.1"
                     /translation="MGRLFGTDGIRGVAGRELTTELAHGLGRALVATVGSRRPRVAVG
                     RDTRASGEMLEAALAAGICSAGGDVLLLGVVPTPAVAFLTVDLGAHAGAVISASHNPP
                     RDNGIKLFSSDGMKLPDMVEDEIERLVREGGGEVAEGLEVGRILRQEDAADRYLGHLA
                     ASVTGTLGGLQIVVDAANGAAYRVAPSLLRRLG"
     gene            635..913
                     /locus_tag="VF097_04075"
     CDS             635..913
                     /locus_tag="VF097_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262008.1"
                     /translation="MEPLVLIHTAANPTAGLLVKSRLESEDIPVFAKGELEGPYRVGP
                     VYLWVPRDLEVQARLVLAEILGGGLELPEGEDVVIEDERLETGPDQDA"
     gene            complement(914..1303)
                     /gene="rpsI"
                     /locus_tag="VF097_04080"
     CDS             complement(914..1303)
                     /gene="rpsI"
                     /locus_tag="VF097_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013875081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX6262009.1"
                     /translation="MAEATRATGRRKESTARVRLTQGSGRFELNGRPLEEYFTSRAQR
                     MIVTAPLRAVEKEKELDVIASLEGGGTSGQAGALQHGIARALVEIDASLRGILKKEGF
                     LSRDAREKERKKYGLKKARKAPQYSKR"
     gene            complement(1306..1770)
                     /gene="rplM"
                     /locus_tag="VF097_04085"
     CDS             complement(1306..1770)
                     /gene="rplM"
                     /locus_tag="VF097_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX6262010.1"
                     /translation="MKTYSPKPSDIERKWWLVDADGATLGRLASEVAQLLRGKHKPTW
                     APHLDVGDHVVVVNASRIRLSGQKREQKVWYRHSQHPGGLRTIPYDRLLRERPDRAVE
                     KAVRGMLPKNRLGRQMIKKLHVYADAEHRHQGQRPEPLDLGATVRRPTEEES"
     gene            complement(1787..2515)
                     /gene="truA"
                     /locus_tag="VF097_04090"
     CDS             complement(1787..2515)
                     /gene="truA"
                     /locus_tag="VF097_04090"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX6262011.1"
                     /translation="MPVLKLVLAYDGTGFRGWARQPGKRTVQGVLERALERMLGEVPR
                     LSVAGRTDAGVHAWGQVVSLEGRDTERLQRGLNGMLAPEVVVRRATRVPDGFDARRSA
                     KARVYRYRVSLAPVPDPFTARYVWHRPAPVALAPMRRAARLLEGEHDFASFCRAPRRG
                     GTVRRLERLAIRREGDLLELGVRGNAFCHQMVRSLVGTLLEVGEGRKEAGSMTEVLKA
                     RSRRAAGRVAPPHGLTLERVLYRP"
     gene            complement(2543..2890)
                     /gene="rplQ"
                     /locus_tag="VF097_04095"
                     /pseudo
     CDS             complement(2543..2890)
                     /gene="rplQ"
                     /locus_tag="VF097_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015491171.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            complement(2897..3823)
                     /locus_tag="VF097_04100"
                     /pseudo
     CDS             complement(2897..3823)
                     /locus_tag="VF097_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166811.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
     gene            complement(3879..>4256)
                     /gene="rpsK"
                     /locus_tag="VF097_04105"
     CDS             complement(3879..>4256)
                     /gene="rpsK"
                     /locus_tag="VF097_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008747488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX6262012.1"
                     /translation="KKKTRKKEKKNVTHGAAHIKSTFNNTIVTITDLEGNTLSWASAG
                     NVGFKGSRKSTPFAAQLTAEAAARRAQEHGVRKVDVYVKGPGSGRETAIRSLAATGLE
                     ILGIQDVTPVPHNGCRPKKRRRM"
BASE COUNT          572 a         1604 c         1398 g          682 t
ORIGIN      
        1 gccgagcctg cgcagcaggc tcggcgccac acggtaggcc gccccgttcg ccgcgtctac
       61 cacgatctgg aggcccccca gcgtcccggt caccgaggcg gcgaggtggc cgaggtaccg
      121 gtcggccgca tcctcctgcc ggaggatgcg gccgacctcg agcccctcgg ccacctcgcc
      181 gcccccctca cgaaccaggc gctcgatctc gtcctcgacc atgtcgggca gcttcatgcc
      241 gtctgaggag aagagcttga ttccgttgtc ccggggaggg ttgtgggacg cggagatgac
      301 cgcgcccgcg tgggcgccga gatccaccgt gaggaacgcc acggccgggg tgggaacgac
      361 ccccagcagg agcacgtccc cgcccgcgga acagatcccc gcggcaagcg cagcctcgag
      421 catctcgccg gaggcgcgcg tgtcccgccc caccgccact cgaggacgcc gactccccac
      481 ggtggccacg agcgctcgcc ccagcccgtg cgccagctcg gtcgtgagct cgcgccctgc
      541 cacgccgcgg atcccgtcgg tgccgaagag tctccccacg ccgcgtatcg taccgacccc
      601 gccgccggcg gcgcgtcggt gccagaatgg gctcatggag ccgctcgtcc tcatccacac
      661 tgcggcgaac ccgaccgcgg ggctgctggt gaagagcagg ctggagagcg aggacattcc
      721 cgtcttcgcc aagggcgagc tcgaaggccc gtaccgggtg gggcccgtgt acctctgggt
      781 gccgcgcgac ctcgaggtcc aggccaggct cgtgctggcc gagatcctgg ggggaggcct
      841 ggagctcccc gaaggcgagg acgtcgtgat cgaggacgag cgcctggaga cgggccccga
      901 tcaggacgcg tgactaccgc ttgctgtact gaggggcctt ccgggccttc ttcagcccgt
      961 acttcttccg ctccttctcc cgggcatcgc ggctgaggaa gccctccttc ttcaggatcc
     1021 cgcggagcga ggcgtcgatc tcgaccaggg ctcgggcgat cccgtgctgg agggcgccgg
     1081 cctgtccgct cgtccccccg ccctcgagcg aggcgatcac gtcgagctcc ttctccttct
     1141 ccacggcccg gagcggggcc gtgacgatca tccgctgggc ccgcgaggtg aagtactcct
     1201 cgagcgggcg gccgttgagc tcgaacctgc cgctgccctg ggtcaggcgg accctggcgg
     1261 tggactcctt cctgcgcccc gtagcgcgcg tggcctcagc catgctcagg actcctcctc
     1321 ggtcggacgt cgtaccgtgg ccccgaggtc gagcggctcc ggtctctgac cctggtgccg
     1381 gtgctccgcg tccgcgtaga cgtgcagctt cttgatcatc tgacggccga gccggttctt
     1441 ggggagcatc ccccgcacgg ccttctccac ggcccggtcg ggacgctccc gcaggagccg
     1501 gtcgtagggg atcgtgcgga gacctccggg gtgctgggag tgccggtacc agaccttctg
     1561 ctcccgtttc tggcccgaga ggcggatcct cgaggcgttg accaccacga cgtggtcccc
     1621 cacgtccagg tgaggggccc acgtcggctt gtgcttcccg cggagcagct gggccacctc
     1681 gctggccaga cgtcccagcg tggccccgtc ggcatcgacg agccaccact tccgctcgat
     1741 gtcactcggc ttcgggctgt aggtcttcat ctctcgtatc cccaggtcac ggcctataga
     1801 gtacccgctc cagcgtcagc ccgtgcgggg gcgccacccg gccggccgct cgccgcgacc
     1861 tcgccttcag cacctccgtc atcgagcccg cctccttcct tccctctccc acctcgagca
     1921 gcgtccccac gaggctccgc accatctggt ggcagaacgc gttccccctg acccccagct
     1981 ccaagaggtc gccctcccgc cggatcgcca agcgctccag gcggcgcacg gtcccacccc
     2041 gccgcggcgc ccggcagaac gaggcgaagt cgtgctcccc ctcgagcagc cgggccgccc
     2101 gccgcatcgg cgccagcgcc accggcgccg gccggtgcca cacgtaccgc gccgtgaacg
     2161 ggtccgggac cggcgccagc gacacccggt accggtacac ccgggccttg gcgctccgcc
     2221 gggcgtcgaa gccgtcgggc acccgtgtcg ccctgcgcac caccacctcc ggggccagca
     2281 tcccgttcag cccccgctgg agccgctcgg tatcccgccc ctccagcgag acgacctgtc
     2341 cccaggcgtg cacgcccgcg tcggtccgtc ccgccacgga gagccggggg acctccccca
     2401 gcatccgctc cagcgctcgc tccaggaccc cctggaccgt gcgcttcccg ggctgacggg
     2461 cccagccccg gaaccccgtc ccgtcgtagg ccaagacgag cttcaggacc ggcacaggcc
     2521 cgggctactc cccctcgcgc tcgaccagct cgatctgggc catctcggcc ccgtcgccct
     2581 tccgagggcc gagcttgagg atccgtgtat agccgccctc ccggtcggcg aaccgggggg
     2641 cgatctcgtc gaagagccga tgaacgacgt cgcgatcggg gaccaccttg agcacctggc
     2701 gccgggcgtg gacgtcgccc cgcttggcga aggtgatcag gcgctcggcc accgggcgga
     2761 gggccttcgc cttcgccacc gtggtccgca gccgctcgtg ggtgaagaga tcggccgcca
     2821 ggccgccgag catcaacccc tggtgcgccg gccccgagcc cagccgtggt cccttcttcg
     2881 gcttcggcac ctactcctcc ttgagcccga ggcccatctc gtcgagcttc gacttgacct
     2941 catcgatcga cttctggccg aagttgcgga tgtccatcag ctccgcctcg gtcctctgga
     3001 cgagctcgcc gaccgtgtcg acgccctcgc gcttcaggca gttgtaggag cggaccgaga
     3061 gctgcagctc ctcgacggga agcgccagct cgcccacggg ctcctcctcg ccctcgggag
     3121 ggccgaggga aaggccgggg gcctcggtga ggtcgctgaa gagagagacg agctcgccca
     3181 gcgtgatccc cgccgaggcc accgcctcgc gggggctcac cgatccgtcg gactcgacgt
     3241 cgaggatcag cttctcccgg tcggtcatct gctcgacccg cgtgttctcg atctggaacg
     3301 tcacccggcg aaccggggag aagatcgagt cgaccgggat cacaccgatc gggtctcgcg
     3361 ccttcttgtt ctgctcggcg ggacgccagc cgacgccccg ctcgaccacc atctccatct
     3421 ccaggccggc gcccttgccc agcgtggcga tgtggagatc ggggttgagg atctcgacgc
     3481 ccgccggggg cgcgatgtcc gcggccgtga cctcgccagg ccccttggcc ttgaggtaca
     3541 tcgtgaccgg ctcgtcgagc tcggagcgga tcaccatctc cttgatgttc aggacgatat
     3601 cggtgacgtc ctcggtcacc ttggggatcg tggagaactc gtggagaacc ccctcgatct
     3661 tgaccgaggt cacggcggcc ccggggatcg aggacagcag ggtgcgcctg agcgagttcc
     3721 ccagggtgtg gccgaagccc ggctcgagcg gctcgatgac gaacgtcgag cggcgctcat
     3781 cgacggtctc ctcctggatc tgcggtcgtg tgacgatcag cacgttcgga acaacctcct
     3841 ccggcggggg atcatccact cctccccgcc aaccggcttc acatgcggcg acgcttcttg
     3901 ggtcggcacc cgttatgggg gaccggggtc acgtcctgga tgccgaggat ctcgagcccc
     3961 gtggcggcca gcgaccggat cgcggtttcg cgccccgatc cgggcccttt gacgtagacg
     4021 tcgaccttgc ggaccccgtg ctcctgggcg cgccgggcgg ccgcctccgc agtcagctgg
     4081 gcggcgaacg gcgtggactt gcgggagccc ttgaacccca cgttgccggc gctggcccac
     4141 gagagcgtat tcccctcgag gtcggtgatc gtgacgatcg tgttgttgaa ggtcgacttg
     4201 atgtgggccg ccccgtgggt gacgttcttc ttctccttct tgcgcgtctt cttctt
//
LOCUS       DASUTZ010000093         7625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_154050, whole genome shotgun sequence.
ACCESSION   DASUTZ010000093 DASUTZ010000000
VERSION     DASUTZ010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7625
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154050"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..425
                     /locus_tag="VF097_04110"
     CDS             234..425
                     /locus_tag="VF097_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262013.1"
                     /translation="MDERRLRELLKKREDEVLSEAETTELEELLAGGGAPEGGPTTRA
                     DRAAPEAPGPGDSEGAPPP"
     gene            422..1426
                     /locus_tag="VF097_04115"
     CDS             422..1426
                     /locus_tag="VF097_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX6262014.1"
                     /translation="MTPSNVDLAELLSREAEAHEERRQRALRRAARSALIWPEEATRL
                     PDLTALRGVGPWIAGILEGWIEEPPEIGELPPERASFLSLAEARRVLDAHPSWRAAIR
                     GDLQVHTTWSDGRADLGEMVATARALGHSYVGVTDHSKGLPIARGMDEETLLRELEEI
                     DELNSKLDGFRALRSLEMNISPDGEGDMDPEVLGRLDLVLGAFHSKLRLREDQTDRYL
                     AAIRNPTVHVLAHPRGRRYGVRTGLVADWERVAEEAAVTGTALELDCFPDRQDLDVET
                     LTVAVEADAWISIGTDAHRPEDLRSFELGLASLALAGVRPERVLNLLPADEVVAWARG
                     "
     gene            complement(1430..1506)
                     /locus_tag="VF097_04120"
     tRNA            complement(1430..1506)
                     /locus_tag="VF097_04120"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1470..1472),aa:Thr,seq:tgt)
     gene            complement(1516..2253)
                     /gene="rlmB"
                     /locus_tag="VF097_04125"
     CDS             complement(1516..2253)
                     /gene="rlmB"
                     /locus_tag="VF097_04125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX6262015.1"
                     /translation="MSRVLVLEGRRLALEAIRSGRAVEVLVAAGARSTPGLRDVLAAA
                     EAAAVPVREVPRDRIRSLSATDSHQGVVARAEAPATLGEADLERRAWAEDARVVVLDG
                     VTDPHNVGAIARTAEAAGASALVLRRRRGAGLTPAALRASAGALLHLPVAGVANISRA
                     LGRLKEAGFWVLGLTGEAETTIDGAAPPPGRLALVLGAEGEGLSRLVRESCDGLVSIP
                     LRGRVASLNVSAAAAVALFGLPTPERR"
     gene            complement(2250..2726)
                     /gene="ispF"
                     /locus_tag="VF097_04130"
     CDS             complement(2250..2726)
                     /gene="ispF"
                     /locus_tag="VF097_04130"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX6262016.1"
                     /translation="MTDLRVGLGFDAHPRDETRPLLLAGVRFEGEAGVAGHSDGDVVC
                     HALADALLGAGALGDIGDHFPDDEPSTEGIAGLILLSEAVRLVRAAGLSPQRCDLVVV
                     AERPRIADRRDEMRANLAEVLGVDPNAVSVKATRPEGLGLVGDGMGCMAIATLSGP"
     gene            complement(2723..3406)
                     /gene="ispD"
                     /locus_tag="VF097_04135"
     CDS             complement(2723..3406)
                     /gene="ispD"
                     /locus_tag="VF097_04135"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX6262017.1"
                     /translation="MRAAAILLAAGRGERLGGERPKALLEVGGTSLLRRAVRTVDAVP
                     EIEGFVVMVPRGLEETAKAQAASSPRFLAAVEGGDSRRASVRRGLNALPDGFDVVVCH
                     DVARPLAAPGLFTAVLRGLEGADGAVPAVPVTDTVKRVGGGWVIETVPRHDLALAQTP
                     QAFRRVALEEAHRAAEAEGLSVTDDAALMERAGRRIAVVPGDAGNVKVTTPEDLERVE
                     AIARIREGS"
     gene            complement(3406..4464)
                     /locus_tag="VF097_04140"
     CDS             complement(3406..4464)
                     /locus_tag="VF097_04140"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEX6262018.1"
                     /translation="MSDRSRRRVLNGGPRGTLVELIRIILVSVFAVGGWQIAERGFEG
                     RTALLIGIALGSMVGYVVGGVIGRLTVRAVREAEAEFEEVPAGEILAGTIGLILGLVI
                     AVLASFLLFRLPPAAAGPTIAFVAVSLSYAGYRIGRAKWADFFSLFGLRLPGAGVRPG
                     EINVVDTSALIDGRVLDVVTAGFLGGVFLVHRGVLDELQRIADSSDPQRRQRGRRGLD
                     VLHDLQRTAEVVLVDEPVQGDVDGVLVRLAKDRGGVVITSDANLAKVAAALDVPVRSM
                     NELAAAVRPPVMAGEELRIHISREGREEGQGVGYLEDGTMVVVEEGRSHLGADVVVTV
                     TNVIQTASGRLLFARLEL"
     gene            complement(4513..5031)
                     /locus_tag="VF097_04145"
     CDS             complement(4513..5031)
                     /locus_tag="VF097_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX6262019.1"
                     /translation="MLFSKGDKVIYPHHGAAVIEDLTTRELLGEKKKYFVLRLTHGDL
                     TLMVPVDNTDEVGLREVVPKREVKKVLEVLKKSETRMPANWSRRYKTNIEKIRSGDIY
                     QVAEVVRNLSIREAEKGLSAGEKRMLAKARQILISELTFAVDTPEEKAEAMVDKVLEE
                     SHKAVASANGSR"
     gene            complement(5149..6486)
                     /locus_tag="VF097_04150"
     CDS             complement(5149..6486)
                     /locus_tag="VF097_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6262020.1"
                     /translation="MGKTRTTFECTACGVPSGQWAGRCPSCGGWGTIEAAMPPSSGTG
                     SVAPPPVSLLATGSDPERRLRVGMEGVDRVLGGGLVPGSVVLVAGEPGIGKSTLLLQI
                     ADRLTGAGHGVLLASGEESHGQVAARARRLGLRSDLAFVPGRELPHVFEAAGAERPAV
                     LMVDSIQAIRDPDHTSLPGGPTQVRLCADRLVGLAKAEGIVVILAGQVTKDGEVAGPR
                     TLEHAVDVVCSFDGDQRTGLRVLAGGKNRFGPEGELAWFEMGAEGLVEADPSELLTSR
                     QAEPGAAVGLLSAGRRAVAVEVQALVAPTEGSPRRHVTGLDPRRFGLVAAVVDRATGL
                     RLGRTELYGATAGGLRIEDPGCDLAVAAALASAATGVPPPGGRGFIGEVSLTGAVRGA
                     PNPAPRLAAAAAAGLTEVYAPPGVRSSDVRVRAVRHLYDALTWAAEGGGRGAA"
     gene            complement(6523..7206)
                     /locus_tag="VF097_04155"
     CDS             complement(6523..7206)
                     /locus_tag="VF097_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HEX6262021.1"
                     /translation="MDRPLTRRRFLAVGLGSLLSGLRPLSGLAAGPKRVDVICRRAWR
                     AAPPGEGLRRHRIRRLTVHHSAIVLSDNRDAPGRFRGHQRAHFGNGWPDIAYHVLIDR
                     GGNVYRGRSPRFRGDTATDYDPKGHLLVLCEGDFDRQSLSGKQVTALVDVLAWACERF
                     GVKPGTIRGHGDWASTACPGRRLRRKIRSGRIRARVRERLRRGGVELRVLCGDAGRRR
                     VRRIERGLA"
     gene            7262..7546
                     /locus_tag="VF097_04160"
     CDS             7262..7546
                     /locus_tag="VF097_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262022.1"
                     /translation="MRTGTARLIIAMGILALTTASCGSPEETTGSQSPEAAPAATSPP
                     PTSPRPTEEPAEDPPVPRELRFTAPALGGGAIDGAEYAGSDLVIWFWAPW"
BASE COUNT         1085 a         2907 c         2649 g          984 t
ORIGIN      
        1 gacgcggagg ctcccatcgt ccatctggac cgggatggcg acctgcagga tgcgcttggg
       61 gtgcttcagg atctcgtgaa cgttggcggg caggtccatg atctgggcgg cctgatcgag
      121 ctgcagcagc gcctgccgcc aaggggaccg cgcctccgtg gccatgccgt gagtgtactc
      181 atcgcggagc ccacgcacga ttgacgtcgg acggcggggg gaatcgaggt gtcgtggacg
      241 agcgaaggct gcgtgagctg ctgaagaagc gcgaggacga ggtcctgagc gaggcggaga
      301 ccaccgagct ggaggagctc ctcgccgggg ggggcgctcc tgaaggcggc ccgaccacga
      361 gggcggaccg cgccgccccg gaggccccgg ggccggggga ctctgagggc gcgccccctc
      421 cgtgacgccc tccaacgtgg acctcgcgga gctgctaagc cgcgaggcgg aggcccatga
      481 ggagcggcgt cagcgagccc tccgccgggc ggcgcggtcc gccctcatct ggccggagga
      541 ggcgacccgg ctcccggacc tcaccgccct ccgcggggtc ggtccctgga tcgcggggat
      601 cctggaggga tggatcgagg agccgcccga gatcggagag ctcccgccgg agcgggcctc
      661 gttcctttcg ctggccgagg cccgtcgcgt gctggacgcc caccccagct ggagggcggc
      721 gatccgcggc gacctccagg tccacacgac gtggagcgac gggcgggccg acctgggcga
      781 gatggtggcg acggcacgcg ccctggggca ctcgtacgtc ggggtgacgg atcactccaa
      841 gggcctgccg atcgcgcgcg gcatggacga ggagacgctc ctgcgggagc tggaggagat
      901 cgacgagctc aactcgaagc tcgatgggtt ccgcgcgctg cgctccctcg agatgaacat
      961 ctcgccggac ggggagggtg acatggaccc cgaggtcctc gggcggctcg atctcgtcct
     1021 gggcgcgttc cactcgaagc tgcgcctgcg cgaggaccag accgatcgct acctcgccgc
     1081 gatccgcaac cccaccgtcc atgttctcgc acacccccga gggaggcgat acggggtccg
     1141 cacagggctc gtcgccgact gggagcgcgt ggcggaggag gcagccgtca cggggacggc
     1201 cctcgagctg gactgcttcc ccgatcgcca ggacctcgac gtggagacct tgacggtggc
     1261 ggtcgaggcc gacgcatgga tctcgatcgg gaccgacgcc caccgtcccg aagatcttcg
     1321 ctcgttcgag ctgggcctgg catccctggc gctggcgggg gtgcggcccg agcgcgtcct
     1381 caacctgctc ccggcagacg aggtcgtggc ctgggcccgg ggatgagcgt ggagctggcg
     1441 gcgggattcg aacccgcgac ctagcgatta caaatcgctt gcgctaccgg actgcgccac
     1501 gccagcgaga cgattctatc ggcgctcggg ggtggggagc ccgaacaggg ccacggccgc
     1561 ggcggcggag acgttgagcg atgccacccg gccccggagc gggatcgaga cgagcccgtc
     1621 gcaggactcc cggacgaggc gcgacaggcc ctcgccttcc gctccgagga cgagcgcgag
     1681 acgccccggg ggcggagcgg cgccgtcgat cgtcgtctcg gcctcgcccg tcaggccgag
     1741 gacccagaag cccgcctcct tcagccttcc cagcgccctc gagatgttgg ccaccccggc
     1801 gaccgggagg tggagcagcg cccctgcgga cgctctgagg gccgcaggag tgaggccggc
     1861 gcctcggcgc cgccggagta cgagggcgga ggcgccggcg gcctcggcgg tcctggcgat
     1921 cgcgcccacg ttgtgggggt cggtcacccc gtcgaggacg acgaccctcg cgtcctccgc
     1981 ccaggcccgc cgctccaggt ccgcctcgcc gagggtcgcc ggcgcctccg ccctcgccac
     2041 cacgccctgg tgcgagtcgg tcgccgagag ggaccggatg cggtctcgag ggacctccct
     2101 gaccgggacc gcggccgcct cggccgccgc cagcacatcg cggaggcccg gggtgctgcg
     2161 ggcccccgca gccacgagga cctcgacggc tcgccccgag cggatcgcct cgagcgcgag
     2221 gcgccgtccc tcgagcacca agacccggct cacggccccg agagggtggc gatggccatg
     2281 cagcccatcc cgtcgccgac gagccccagg ccctcgggcc tcgtggcctt cacggacacc
     2341 gcattgggat ccacgcccag gacctccgcg aggttggccc gcatctcgtc gcgtcggtcg
     2401 gcgatgcgcg ggcgctccgc gacgactacg agatcacagc gctgagggct cagccccgcg
     2461 gcccggacca gccgcaccgc ctcggacagc aggatgagac cggcgatccc ctcggtggag
     2521 ggctcgtcgt cggggaagtg atcgccgatg tcgccgaggg cccccgcgcc gagcagggcg
     2581 tcggccaggg cgtggcagac cacgtccccg tcggagtggc cggccacgcc ggcctctccc
     2641 tcgaacctca ccccggccag gaggagtggc ctcgtctcgt cgcgcgggtg cgcatcgaag
     2701 cccaggccga cgcggagatc cgtcacgagc cctcccggat ccgggcgatc gcctcaaccc
     2761 gctccaggtc ctccggggtg gtgaccttga cgttgcccgc gtcgccgggg accaccgcaa
     2821 tcctgcgtcc ggcccgctcc ataagcgccg cgtcgtcggt cactgagagg ccctccgcct
     2881 ccgcggcccg atgggcctcc tccagggcca cgcgacggaa cgcctggggc gtctgggcca
     2941 gcgccaggtc gtggcggggg accgtctcga tcacccatcc gccgccgacc cgcttcacgg
     3001 tatcggtcac gggcaccgcc ggcacggcgc cgtctgctcc ctccagcccc ctcaggacgg
     3061 ccgtgaagag cccgggggcg gccaggggcc gcgccacgtc gtgacatacg accacgtcga
     3121 agccgtcggg cagcgcattc agccctcggc gtacggatgc ccgccgcgag tcacccccct
     3181 ccaccgcggc gaggaagcgg ggcgagctcg cagcctgggc cttcgccgtc tcctcgagcc
     3241 cccgagggac catcaccacg aacccctcga tctcggggac ggcgtccacc gtgcgcacgg
     3301 cccgccgaag gagcgaggtg ccgcccacct cgagaagcgc cttgggccgt tccccgccca
     3361 gccgctcgcc ccggcccgca gccaggagga tggccgccgc ccgcatcaca gctcgaggcg
     3421 ggcgaacagg agccgccccg aggccgtctg gatcacgttg gtcaccgtca ccacgacgtc
     3481 ggcgcccagg tgggaccggc cttcctccac gacgaccatg gtgccgtcct ccaggtagcc
     3541 cacaccctgg ccctcctcac ggccctcccg gctgatgtgg atccgcagct cctcaccggc
     3601 catcaccgga ggccgcaccg ccgccgcgag ctcgttcatc gagcggacgg ggacgtcgag
     3661 cgctgcggcc accttggcca ggttcgcgtc ggaggtgatg accacgcccc cccgatcctt
     3721 ggccaaacgg accagcacgc cgtccacgtc gccctggacc ggctcgtcca cgagcacgac
     3781 ctcggccgtc cgctggaggt cgtgcagcac gtccagcccc cgccgcccgc gctgccgccg
     3841 ctggggatcc gaggagtctg cgatccgctg gagctcgtcg aggacccctc ggtgcacgag
     3901 gaagaccccg cccaggaacc cggccgtgac gacgtccagc actcgcccgt cgatcagcgc
     3961 gctcgtgtcc acgacgttga tctcgcccgg ccgcacgcct gcccccggca ggcggagccc
     4021 aaagagcgag aagaagtccg cccacttggc ccggccgatc cggtagcccg cgtacgagag
     4081 cgagacggcc acgaacgcga tggtgggccc ggcagccgcg ggcgggaggc ggaacagcag
     4141 gaacgaggcg aggacggcga tcaccaggcc caggatgagc ccgatcgtcc ccgccaggat
     4201 ctcgcccgcg gggacctcct cgaactcggc ctcggcctcg cggacggctc ggaccgtgag
     4261 cctcccgatc acgccaccca ccacataccc caccatgctg cccagggcga tcccgatcag
     4321 gagggccgtc cggccctcga aaccgcgctc cgcgatctgc cacccgccga cggcgaagac
     4381 ggagacgagg atgatcctga tgagctcgac gagggtgccc cgggggccgc cgttcaggac
     4441 gcggcgtctg ctccgatcgc tcacgggtcc acggtgcgcc gccggaggcg cggagacaag
     4501 ttcccctggg gcctacctcg agccgttcgc ggaggcgacg gccttgtgcg actcctcgag
     4561 caccttgtcc accatcgcct cggccttctc ctcgggcgtg tccaccgcga acgtgagctc
     4621 ggagatcagg atctgacggg ccttggccag catccgcttc tcgccggccg agaggccctt
     4681 ctcggcctcg cggatcgaga ggttccgcac gacctcggcc acctggtaga tgtcgccgga
     4741 gcggatcttc tcgatgttgg tcttgtagcg ccggctccag ttcgcgggca tgcgagtctc
     4801 ggacttcttc agaacctcca ggaccttctt cacctcgcgc ttgggaacca cctcgcggag
     4861 gcccacctcg tcggtgttgt ccaccggaac catcagggtg aggtcaccgt gtgtgagcct
     4921 gaggacgaag tacttcttct tctctccgag gagctctcgg gtcgtgagat cctcgatcac
     4981 ggccgcgccg tggtggggat agatgacctt gtcacccttg ctgaacaaca agtacccccc
     5041 tgagtcttgg acggacaggc tctcatgata gcagggagtc acctcccccg caaatgaaaa
     5101 ctgcaggtca cgggccattt ccgggttgtc cggcggcgtt tcgcctgctc aggcggcccc
     5161 tctgcccccg ccctcggccg cccatgtgag ggcgtcatac aggtgtcgga ccgccctgac
     5221 cctgacgtcg gacgacctca ccccgggagg ggcgtagacc tcggtgagcc cggcggccgc
     5281 ggctgcggcg aggcgggggg ccgggttcgg ggcgccccgc accgctcccg tcaggctcac
     5341 ctcgccgatg aagccgcggc cgccgggcgg gggaaccccc gtggcggccg aggccagcgc
     5401 ggcggcgacc gccaggtcgc agcccgggtc ctcgatcctg aggccacccg ccgtggcccc
     5461 gtacagctcg gtccgtccca gcctgaggcc ggtggcccgg tcgaccaccg cggccacgag
     5521 gccgaacctc ctgggatcga gccccgtcac gtgtcgccgg ggggatccct cggtgggggc
     5581 caccagcgcc tggacctcca cggcgaccgc gcgccgtccc gcggacagca gccccaccgc
     5641 cgcgcccggc tccgcttgcc tgctggtcag cagctcggag gggtcggcct ccaccaggcc
     5701 ctcggcaccc atctcgaacc acgccagctc gccctcgggc ccgaagcggt tcttcccgcc
     5761 ggccagcacg cggaggccgg tccgctggtc cccgtcgaac gagcagacga cgtccacggc
     5821 gtgctccagc gtcctcgggc cggctacctc gccgtccttg gtgacctggc cggccaggat
     5881 gacgacgatc ccctcggcct tggccagccc tacgaggcgg tcggcgcaca ggcgcacctg
     5941 cgtcggccct ccgggcaggg acgtgtggtc cgggtccctg atcgcctgga tcgagtcgac
     6001 catcagcacg gccggccgct cggccccggc ggcctcgaac acgtgcggca gctcgcggcc
     6061 cgggacgaac gcgaggtccg accgcaggcc caggcgcctg gcccgcgcgg ccacctggcc
     6121 gtgggactcc tcccccgagg ccagcagcac cccgtgcccg gcacccgtca gccggtcggc
     6181 gatctggagc aggagcgtcg acttcccgat gcccggctca ccggccacga ggacgaccga
     6241 gcccggaacg agccctcccc ccagcacccg atccacgccc tccatgccga cccggagccg
     6301 cctctccggg tcgctccccg tcgccagcag cgacaccggg ggaggcgcaa ccgatccggt
     6361 cccggaggaa gggggcatcg cggcctcgat cgtcccccag cccccgcagg acggacaccg
     6421 cccggcccac tgaccggacg ggacgccgca ggccgtgcac tcgaaggtcg tgcgcgtctt
     6481 ccccacgggg tcaccctagc ccccgggtct gtcacgaacg gctcaggcca ggccccgctc
     6541 gatccgcctg acccgccgcc tccccgcgtc gccgcacagc acccgcagct ccaccccgcc
     6601 ccggcgcagc cgctccctca cgcgggcccg gatgcgcccg ctcctgatct tgcgccgcag
     6661 gcgccgcccg gggcacgccg tcgaggccca gtcgccgtgg ccgcggatcg tgcccggctt
     6721 cacgccgaag cgctcgcagg cccacgccag gacgtccacg agcgccgtga cctgcttccc
     6781 cgagagcgac tgccggtcga agtcgccctc gcacaggacg aggaggtggc ccttcggatc
     6841 gtagtcggtg gcggtgtccc ctcggaacct cgggctcctc cccctgtaca cgttgccgcc
     6901 tcggtcgatc agcacgtggt aggcgatgtc cggccatccg ttgccgaagt gcgcccgctg
     6961 gtgtccgcgg aaccggccgg gggcgtcgcg gttgtccgag agcacgatgg ccgagtgatg
     7021 gaccgtgagc cgccggatcc gatgccgcct caggccttcg ccgggaggag ctgcccgcca
     7081 ggcccggcga cagatcacgt cgacgcgctt ggggccggcg gcgagcccgg agagcgggcg
     7141 caggcccgag aggagggatc cgagccccac cgccaggaag cgccgccggg tgagtggccg
     7201 atccacggcg tcatgctatc cgtaaggctc tcgaaatccc ctccctccct atacttggcc
     7261 gatgcgaacc ggcacggctc ggctcatcat cgcgatgggg atcctggcgc tcacgacggc
     7321 ctcgtgcgga tccccggaag agacgacagg ctcccagagt cccgaggcgg cgcccgcggc
     7381 cacgagcccg ccgcccacct cgccccgacc caccgaggaa ccggccgagg acccccccgt
     7441 ccccagagag cttcgcttca cggcaccggc gctgggaggg ggtgccatcg acggcgccga
     7501 gtacgcgggc agcgacctcg tgatctggtt ctgggccccg tggtgaggga cgtgcaaccg
     7561 ggaggccccc ggcgtcgctc aggctgagcg tcggaacgac ggagtcgagt tcctggggat
     7621 cgcgt
//
LOCUS       DASUTZ010000094         3378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_285032, whole genome shotgun sequence.
ACCESSION   DASUTZ010000094 DASUTZ010000000
VERSION     DASUTZ010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285032"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..640
                     /locus_tag="VF097_04165"
     CDS             50..640
                     /locus_tag="VF097_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262023.1"
                     /translation="MRGSRLRWLIPTVVLISIVLIPLLAWGQTPPPGEEPEVVRPWTW
                     WLAIPLALAVLGLVALIALRYVRFSPRFFGKEEAPKIPERPRAYAGVNAPAYPAPAPQ
                     PLPAAAPAAAARPAATTATAEAPAPAQAPAAEPEPESQPEPAADAQAPEKEAEPSGEG
                     DDVYERVLKEQLDKGVSPKVAEGRAKAAAHRARRSG"
     gene            643..1683
                     /locus_tag="VF097_04170"
     CDS             643..1683
                     /locus_tag="VF097_04170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX6262024.1"
                     /translation="MGAAAKLADWGTARRIGVTFSGVGPIIAPVDRARLSEDFAEAVS
                     RADSLVAELTGLPIDGPATRPWVMTRDEWVRQNVRGFETLLDPVADRLTSDGDGPMAA
                     VRRKFLAAQVGGLLGYLSRRVLGQYDLFLPPDDRDLLYFVGPNVISVERRHRLQPEEF
                     RLWLSLHEVTHRFQFGGVPWLRGYLRGLVDEYVESLDLDPRRLLEAFRRAREEAARSS
                     AWRGVGILFLLMTPEQRDTFRRMQALMSLLEGHGNHVMHVLSEGRIRGAPRFERVLRE
                     RRRSAGAGRAFQRAIGFEAKARQYGEGERFIAAVVERAGVEGFSRVWEGPEHLPTLEE
                     VSDPEAWVRRVI"
     gene            1685..2719
                     /gene="tilS"
                     /locus_tag="VF097_04175"
     CDS             1685..2719
                     /gene="tilS"
                     /locus_tag="VF097_04175"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX6262025.1"
                     /translation="MAGRPPAVARVLERATRTIRRHEMLPPGRAVLVACSGGPDSICL
                     LHALHRLRRLFRIRLEVFHFDHRLRRTSSEHAAYVRGQARRLGLPFHLRAADGGPARG
                     ESPEAWARLARYAALTEVATERGIERAAVAHTRDDQAETVLLGLVRGGGLEAVAGMDP
                     VATVPPLALTVVRPLLEVTRDEIVAFCRSLRLRPREDPTNRDPRFLRNRVRHRVLPHL
                     ERELGRNLRATLARVAANVREDAAYLDQVASAAAREVVEVDEGEVRMRVARLRALVPA
                     VGSRVARDALRVAAAMAGSWTADVEAAHVEAILDLARGRPGRRIDLPGALMGWRDREY
                     VRLRPAGEAT"
     gene            2716..3318
                     /gene="hpt"
                     /locus_tag="VF097_04180"
     CDS             2716..3318
                     /gene="hpt"
                     /locus_tag="VF097_04180"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX6262026.1"
                     /translation="MTGDGSGRAATLTGHVAEYEQDIERILITEDEIQGKLKELGERI
                     TEDYRGHDLVLVGVLKGAFVLMADLSRHIRLPIQFDFMAISSYGAATKSSGVVRILKD
                     LDHEIEGRDVLVVEDIVDSGLTLQYLLRNLQARKPASLEVAALLLKTEVDRVPVTIRY
                     TGFEIPPEFVVGYGLDYAERYRNLPFVGTLKPQVYETLVE"
BASE COUNT          439 a         1223 c         1252 g          464 t
ORIGIN      
        1 acacgatcgc gggaggcccg tgaacgggga tttgttagaa tccgcgccga tgcgcgggtc
       61 ccgtctgcgg tggctgatcc ccacggtggt cctcatctcc atcgtgctga tccccctgct
      121 cgcctggggg cagacgccgc cgcccggcga ggagcccgag gtggtccgcc cctggacctg
      181 gtggctcgcg atcccgctgg ccctggcggt cctgggcctc gtggccttga tcgccctgcg
      241 gtacgtgcgg ttctccccgc ggttcttcgg gaaggaggag gcgccgaaga tccccgagcg
      301 cccacgcgcc tacgccggcg tgaacgcccc ggcctatccc gccccggccc cccagccact
      361 ccccgccgcc gcgccggcgg ccgccgcacg cccggcggcc acgacggcca cggccgaggc
      421 ccccgccccc gcgcaggccc ctgcggcgga gcccgagccc gagtcccagc ccgagcccgc
      481 cgcggacgcc caggcccccg agaaggaggc cgagccctcg ggcgaaggcg acgacgtgta
      541 cgagcgcgtt ctgaaggagc agctcgacaa gggcgtctcc cccaaggtgg ccgagggacg
      601 ggccaaggcc gccgcccacc gcgcccgtcg gtcgggctga ggatgggggc cgcggcgaag
      661 ctggccgact gggggacggc ccgcaggatc ggcgtcacgt tctcgggagt gggccctatc
      721 atcgcgccgg tcgaccgggc gcgcctgagc gaggacttcg cggaggccgt ctcccgggcg
      781 gactcgctcg tcgccgagct gacgggtctc ccgatcgacg ggccggccac gcgcccctgg
      841 gtgatgaccc gggacgagtg ggtgcggcag aacgtccgag gcttcgagac gctcctggac
      901 ccggtcgcgg accggctcac gagcgacggg gacggcccga tggctgccgt ccgcaggaag
      961 ttcctagcgg cccaggtcgg gggcctgctc ggctacctga gccggcgggt cctgggccag
     1021 tacgatctct tcctgccccc cgacgaccgg gacctcctct acttcgtggg gccgaacgtg
     1081 atctcggtgg agcgccgcca tcggctccag ccggaggagt tccggctgtg gctctcgctc
     1141 cacgaggtga cgcaccgctt ccagttcggc ggcgtaccgt ggctccgggg ctacctgcgg
     1201 ggactcgtgg acgagtacgt ggagtccctc gacctcgatc cccgacgcct cctcgaggcg
     1261 ttccggaggg cccgtgagga ggcggctcgg agctccgcct ggcgcggggt ggggatcctc
     1321 ttcctcctga tgacgccgga gcagcgtgac accttccgga ggatgcaggc gctgatgtcg
     1381 ctcctcgagg ggcacggcaa ccacgtgatg cacgtgctct cggagggccg aatccgcggg
     1441 gctcccaggt tcgagcgggt gctgcgggag cggcgccggt ccgctggcgc cggtcgggcc
     1501 ttccagcggg cgatcggctt cgaggcgaag gcgcggcagt acggcgaggg cgagcggttc
     1561 atcgcggcgg tcgtggagcg ggccggggtg gagggtttca gccgggtgtg ggaaggaccc
     1621 gagcacctcc ccacgctgga agaggtttcc gaccccgagg cctgggtccg ccgggtcatc
     1681 tgacatggcc gggcgcccgc ccgccgtggc gcgggtgttg gagcgagcga cccgcacgat
     1741 ccgaaggcac gagatgctgc cccccggacg ggcggtgctc gtggcctgct ccggcggccc
     1801 ggactcgatc tgcctgctcc acgccctgca ccgcctccgg cgtctgttca ggatccgcct
     1861 ggaggtcttc cacttcgatc accggctccg ccggacgtcg agtgagcacg cggcctacgt
     1921 gcggggccag gcccggaggc tcggcctgcc gttccacctc cgggcagcgg acggcggacc
     1981 ggctcgcggg gagtccccgg aggcctgggc ccggctggct cgctacgcgg cgctgaccga
     2041 ggtcgccacc gagcggggga tcgagcgggc ggccgtggcc cacacgcggg acgaccaggc
     2101 cgagaccgtc ctgctgggtc tggtccgagg gggcggcctc gaggcggtgg ccggcatgga
     2161 cccggtggcg accgtgccgc cgctcgccct caccgtggtc cgaccgctgc tcgaggtcac
     2221 ccgggacgag atcgtggcgt tctgtcgctc cctgcgactc cggccccgcg aggaccccac
     2281 gaaccgcgac ccgcgcttcc tccggaaccg ggtccgtcac cgggtgctgc cgcatctcga
     2341 gcgggagctc ggccggaacc tgcgggccac gctggcccgt gtcgcggcca acgtccgtga
     2401 ggatgccgcc tacctcgacc aggtcgcgtc ggcggccgcg cgcgaggtcg tggaggttga
     2461 cgagggcgag gtccggatgc gcgtcgcgcg cctccgagcc ctggtgccgg cggtgggctc
     2521 gcgggtggcg cgcgacgcgc tccgtgtggc cgcggccatg gcgggttcct ggaccgccga
     2581 cgtcgaggcg gcccacgtgg aggccatcct cgacctcgcg cgcggccgcc ccggccgccg
     2641 gatcgatctc cccggcgcct tgatggggtg gcgggacagg gagtatgttc ggctcaggcc
     2701 ggcgggggag gcgacgtgac gggcgacggc agcggccggg cggctacact gacgggccac
     2761 gtggccgagt acgagcagga catcgaacgg atcctcatca ccgaggacga gatccaaggc
     2821 aagctgaagg agctcggcga gcgcatcacc gaggactatc ggggacacga cctcgtgctc
     2881 gtgggagtgc tgaagggcgc gttcgtgctg atggccgacc tctcccgcca catccgcctt
     2941 ccgatccagt tcgacttcat ggcgatctcg tcctacggcg ccgccacgaa gtcgtccggg
     3001 gtggtccgca tcctgaagga cctcgaccac gagatcgagg ggcgggacgt cctcgtcgtc
     3061 gaagacatcg tcgactcggg cctgaccctc cagtacctcc tgcggaacct ccaggcgagg
     3121 aagcccgcca gcctcgaggt cgccgcgctc ctcctcaaga ccgaggtgga ccgggtcccg
     3181 gtcacgatca ggtacacggg gttcgagatc ccccccgagt tcgtcgtggg ctacggtctc
     3241 gactacgcgg agcgctaccg caacctcccc ttcgtgggga cgctgaagcc gcaggtctac
     3301 gagacgctcg tcgagtagcc ggcgcgggcc accgccgccc gggcccgcag caggaagaag
     3361 aaccccgccg cccccacc
//
LOCUS       DASUTZ010000095         2707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1709975, whole genome shotgun sequence.
ACCESSION   DASUTZ010000095 DASUTZ010000000
VERSION     DASUTZ010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1709975"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..829)
                     /locus_tag="VF097_04185"
     CDS             complement(86..829)
                     /locus_tag="VF097_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX6262027.1"
                     /translation="MRRSLAIIGVTLALGAASCAPSQPAAEDVRLGSEESSPGSGSGQ
                     GSSQGSPKRVDPRRDGFEVGLGEWAVTLEAAAIRPGPVTFVVHNRGTMVHGFEMEIEN
                     VDDGFKVETQTFGPGETVTLDLDLAPGLYKVECLIDGHDDLGMEALLEVRPDAPKVRQ
                     SRPSAEGSAVSIQGFAFDPSELSVAAGDEVTWTNDDPAPHTVTARDGSFDSGTIEPGQ
                     SFSATVDASGTVTYFCVIHPTMEGTIRVG"
     gene            complement(826..1173)
                     /locus_tag="VF097_04190"
     CDS             complement(826..1173)
                     /locus_tag="VF097_04190"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX6262028.1"
                     /translation="MRYLTRTVVVVGALLALTSCGGDGDDAGGGQGEASLSMVENAFQ
                     PAELTVAAGSELSVSNDGQAVHNITIEGTSIDEDVDAGQSTTITIDAEPGEYTMFCEF
                     HRAAGMEGTVTVQ"
     gene            1299..1371
                     /locus_tag="VF097_04195"
     tRNA            1299..1371
                     /locus_tag="VF097_04195"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1332..1334,aa:Ala,seq:tgc)
     gene            complement(1409..1726)
                     /locus_tag="VF097_04200"
     CDS             complement(1409..1726)
                     /locus_tag="VF097_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262029.1"
                     /translation="MLLIVGLALLATPRAASADKPSAVCPPGFNLGALDFESSLSLSR
                     SKAGLEDGFFTSEGLETSFDFFDKNGDGLVCFQESVGSTQANPASLWQYLYNVVDNSA
                     SKP"
     gene            complement(1862..2290)
                     /locus_tag="VF097_04205"
     CDS             complement(1862..2290)
                     /locus_tag="VF097_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6262030.1"
                     /translation="MKPGIRPVTADDAEAVARLSTQLGYPATAEEIVERLAGLPSHHA
                     VLVAELGGAVVGWIQVQESRLVVERLRADITGLVVDQTARREGVGRRLVEAVERWAAD
                     RGAERIRVRSNVVREDAHAFYPSLGFEIAKTSTVFEKRLS"
     gene            complement(2287..>2707)
                     /locus_tag="VF097_04210"
     CDS             complement(2287..>2707)
                     /locus_tag="VF097_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016329434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HEX6262031.1"
                     /translation="LPEALPEGTVAVYAAIEAEVSAMDPEEGAALLREFGVEEPGLRK
                     LIRAAYDALDLITFLTTGEDETRAWQVRRGALAPEAAGQIHTDLERGFIRAEVVTYDQ
                     LVAAGSMKVAKERGLVRTEGKAYEVQEGDVVNILFSV"
BASE COUNT          408 a          974 c          888 g          437 t
ORIGIN      
        1 cggaaggatt ccgtaccggc cgcgcccccc cggccgtcca tcaccctgta gacgcccacc
       61 ggcatgccgt cagtatgccg cgacgtcacc cgacgcggat cgtcccctcc atcgtggggt
      121 ggatcacgca gaagtaggtg accgtcccag aggcgtccac cgtagccgag aagctctgcc
      181 ccggctcgat cgtgcccgag tcgaaggacc catccctcgc ggtcaccgtg tggggggcgg
      241 ggtcgtcgtt ggtccaggtg acctcgtcgc cggccgcgac cgagagctcg gacgggtcga
      301 aggcgaagcc ctggatcgag acggcgctcc cttcggccga gggccgggat tgccgcacct
      361 tcggggcgtc gggccgcacc tcgaggagtg cctccattcc caggtcgtcg tggccgtcga
      421 tcaggcactc gaccttgtag agccctgggg cgaggtccag gtcgagcgtg accgtctcgc
      481 ccgggccgaa cgtctgcgtc tcgaccttga agccgtcgtc gacgttctcg atctccatct
      541 cgaagccgtg caccatcgtg ccgcggttgt ggaccacgaa cgtgacgggc ccggggcgga
      601 tcgccgccgc ctccagggta acggcccact ccccgagccc gacctcgaag ccgtcccggc
      661 gcgggtcgac ccgcttcggg gagccctggg acgaaccttg tccggatccc gaccccgggg
      721 agctctcctc gctccccagg cgaacgtcct cggcggccgg ctgggagggg gcgcaggagg
      781 ccgccccgag cgcgagcgtc acgccgatga tcgcgagcga gcgcctcact ggaccgtcac
      841 cgtgccctcc atcccggccg cgcggtggaa ctcgcagaac atcgtgtact cgccgggctc
      901 cgcgtcgatc gtgatggtcg tgctctggcc cgcgtcgacg tcctcgtcga tcgaggtgcc
      961 ctcgatcgtg atgttgtgga ccgcctggcc gtcgttcgag acggagagct cgctccccgc
     1021 cgccaccgtg agctcggccg gctggaaggc gttctccacc atcgagagcg acgcctcgcc
     1081 ttgaccgcca cccgcatcat cgccgtcgcc gccgcaggag gtgagggcca agagcgctcc
     1141 gaccaccacc accgttctcg tcaggtacct catccgtgcc tccttctcgt tgacacggtg
     1201 atcttcgagc ccgggcatgg gcctcgcctg aagcggccct gaaggttcct tcaggttggc
     1261 ccgggtcgtc gagcggctga ggctagacta ctgaggacgg ggacgtagct cagctggtag
     1321 agcgcctgct ttgcaagcag gaggtcgtcg gttcgaatcc gatcgtctcc acgttgcccc
     1381 ccaagcgtgg gaccgaacgc cgaaggatct agggcttgga agcgctgtta tccacgacgt
     1441 tgtagaggta ctgccatagt gaggccggat tggcctgcgt cgaccccacg ctctcctgga
     1501 agcagaccag cccgtctccg ttcttgtcga agaaatcgaa cgaggtctcc agcccctcgc
     1561 tcgtgaagaa cccgtcctcg aggcccgcct tcgagcgcga gaggctcagc gagctctcga
     1621 agtcgagtgc gccgaggttg aagccgggcg ggcatacggc cgacggcttg tccgcgctcg
     1681 ctgctctcgg agtggcaagg agcgctagac cgactatcag gagcacgatg actcgcgatc
     1741 tcctctgcgt gacagacgag gtcatgagga tcacctccag gcgtcacgta gcgcgatcgc
     1801 gcctccaacg tgtccgccac ggtcaacatc ataccgacag cgctccttcc agccaaaccc
     1861 gtcaggagag acgcttctcg aagacggtgg aggtcttggc gatctcgaat ccgagcgacg
     1921 ggtagaaggc atgggcgtcc tcgcggacca cgttggagcg gacccggatc cgttcggcgc
     1981 cgcggtcggc cgcccatcgc tcgaccgcct ccacgagccg tctgcccacg ccctctcgcc
     2041 gggccgtctg gtcgaccacg aggcccgtga tgtcggcgcg gaggcgctcc acgacgaggc
     2101 gcgactcctg cacctggatc cacccgacca cggcgccgcc gagctcggcc accagcacgg
     2161 cgtggtggga aggtagcccg gccaggcgct ctacgatctc ctcggcggtc gccgggtagc
     2221 cgagctgggt ggagaggcgc gccaccgcct ccgcgtcgtc cgcggtgacg ggccggatcc
     2281 cgggcttcac accgagaaga ggatgttcac cacgtccccc tcctgcacct cgtaggcctt
     2341 gccctcggtc ctcacgaggc ctcgctcctt ggccaccttc atcgacccgg cggcgaccag
     2401 ctgatcgtag gtcaccacct cggcccggat gaagccccgc tccagatccg tgtggatctg
     2461 cccggcggcc tcgggcgcca gcgccccccg gcggacctgc caggctcgcg tctcgtcctc
     2521 cccggtcgtg aggaacgtga tgaggtcgag cgcgtcgtac gcggcccgga tcagcttccg
     2581 caggccgggc tcctcgaccc cgaactcacg gaggagggct gcgccttctt cggggtccat
     2641 ggcggagacc tccgcctcga tcgctgcata gaccgcgacg gttccctcag ggagagcctc
     2701 gggcagg
//
LOCUS       DASUTZ010000096         5237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_414791, whole genome shotgun sequence.
ACCESSION   DASUTZ010000096 DASUTZ010000000
VERSION     DASUTZ010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5237
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414791"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..924)
                     /locus_tag="VF097_04215"
     CDS             complement(118..924)
                     /locus_tag="VF097_04215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX6262032.1"
                     /translation="MLSFDQGFFGPGSVSWQVHGELTVLFGGARALLMQSAHPLVIAG
                     ARETGFYKRSPWKRLQRTLMLNYAITFGTKKDARAAAEHINEVHRRVHGIDPVTGQAY
                     HGLDPDLLLWVHATLVHSALLFERLTVGRLDAAGRQRFHEEQMLVGEMLELPREKIPP
                     TVEELSEYMRDVVAGDTLIVTDAALEVADLFREFPPEAEWKPVLRAISFWSFATLPPK
                     LREAYGVRWSPARDVAARVSLAALKRLRPAIPRRYRRILIAEQARIRTSG"
     gene            963..1844
                     /locus_tag="VF097_04220"
     CDS             963..1844
                     /locus_tag="VF097_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262033.1"
                     /translation="MELLTATDGGVISLPGGERLGPERVTFLHRAGEVTVEVRGGHEV
                     VVRSNGEAPGLRSDLRANCALALAGGGALVGTAEARLFLARDGSLEAVEDFDRAEGRE
                     RWHTPWGGPPDVRSLSAGPDGVVYVNVHVGGILRSENGGATWSPTAIPIEEDVHQVLA
                     HPERAGLVLAATAYGLARSEDGGESWATIDEGLDRRYSRAVAAAGDTVLLSASSGPGG
                     GEAALYRLAGDRFEKCDLPDPFGSNLDTHRLVADGDTVAAALPNGDVFLSEDSGRSWE
                     RIADALPSVRCLLLEGR"
     gene            1841..2824
                     /locus_tag="VF097_04225"
     CDS             1841..2824
                     /locus_tag="VF097_04225"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEX6262034.1"
                     /translation="MRRPTPLNPLIRRLLWPAALLLGVTTYGVAGYMLIEGWSFLDAL
                     YMTIITLSTVGFREVQPLEASGRVFTLSLVVLGVTAVLVGLTLIGIWISEGNLGERRR
                     RKRMEQLIDRLEDHFVVCAFGRVGRTVARRFLDAEVPFVVVDRDEALEARMVDMNVPH
                     LIGDPSDEEVLRQAGIDRARGLVCAVDSDATNVFITLTARALKPDLFIVARAATSESI
                     PRLEKAGADRVVSPYVTSGEAMALMSLRQDVVGMLDVADLGDREVRVEELVVSSGSHL
                     EGLRVDEAAGRALALALRHADGSTTVGPPADRPLKAGDRLLIMADERTLQA"
     gene            complement(2887..3474)
                     /locus_tag="VF097_04230"
     CDS             complement(2887..3474)
                     /locus_tag="VF097_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018653217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262035.1"
                     /translation="MKTIERRDVAPAPELYIPEAEVPVKGTRIWAILRISFGWVFLWA
                     FLDKLLALGFSTGRNEETGVIDFFGEAAWINGASPTEGFLAFGTKGPFADFFQGLAGS
                     AWVDWVYMLSMAAIGLALILGIGMRLAAIGAIIWLGLFYVAGSIWPENNPFIDDHVIY
                     AIALAGLAYIGAGRTWGLGRWWESTALVKKFPVLK"
     gene            complement(3521..4153)
                     /locus_tag="VF097_04235"
     CDS             complement(3521..4153)
                     /locus_tag="VF097_04235"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6262036.1"
                     /translation="MLVRDVMTVGVVTAAPDTPVKELAALMVGARISGIPVVEDGKVV
                     GIVSESDLLPLHEGDPGRRVQVAADVMTRKLVTLVEGMTVTEAARVLQRHGVKRAPVM
                     RGERLVGICTRANLLRPYLRTDLEILAEVEEAVLSGALGISPRGVRALVTDGVVRLEG
                     RVTSARTRAILLRLVRAVDGVVDLEEQLEIEEEPNGQTTHPEGAGRPVTD"
     gene            complement(4172..4909)
                     /locus_tag="VF097_04240"
     CDS             complement(4172..4909)
                     /locus_tag="VF097_04240"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6262037.1"
                     /translation="MKKLKVEDVMTRTVVAVREDTPFKDVVRMMLEHRVSGVPVLDGD
                     GLLAGIVTEADLLTLEEGKVEPKHRRSFLEWLVQPGRLAEIEADAAEVRAGDIMTPSV
                     VTVRPETTVPEAAKLMLDAQVKRLPVIDEEGKVLGIVSRRDLLQPFLRTDEEIEREIS
                     EEVVFRTMWIDPDTIGVEVERGCVRLRGTVERRSVAEILVELVYRVEGVVGVEDELGY
                     RWDDRKVKPRGPFSAPRWSENWARRGA"
     gene            complement(4912..>5237)
                     /locus_tag="VF097_04245"
     CDS             complement(4912..>5237)
                     /locus_tag="VF097_04245"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6262038.1"
                     /translation="DAAGRMQYQEIGSLAVLEHGKLVGILTERDLARALADGVDPAET
                     PVAAYMTEEPVTVTPEADAASVAATMLDLGVRHIPVVEGGQVIGMVSSRDLLSLEAWN
                     AIGVT"
BASE COUNT          758 a         1921 c         1781 g          777 t
ORIGIN      
        1 ggacgtcacg cggatggcgc tcatggccca ggcggtcggg ctcgtggcgc tgctcgtgat
       61 ccgctcgatc gtgaaatcga gggcgaaggc caaggcccgg atcgcgaagg ccgagagcta
      121 gccggaggtc cggatgcggg cttgctcggc gatcaggatc cgccggtagc gccgggggat
      181 cgccgggcgg aggcgcttca gcgcagccaa gctgacccgg gcagccacgt cgcgggcggg
      241 gctccaccgc accccgtagg cctcgcgcag cttcgggggg agcgtggcga acgaccagaa
      301 ggagatcgcg cgcaggaccg gcttccactc cgcctccggc gggaactccc ggaacaggtc
      361 ggccacctcg agcgcggcgt cggtgacgat cagcgtgtcg ccggccacga cgtcccgcat
      421 gtactccgag agctcctcca ccgtgggcgg gatcttctcg cggggaagct cgagcatctc
      481 cccgaccagc atctgctcct cgtggaagcg ctggcgccct gcggcgtcga gcctccccac
      541 cgtcaggcgc tcgaacagca gggcgctgtg gacgagcgtg gcgtggaccc acagcaagag
      601 gtcggggtcg agaccgtggt aggcctggcc ggtcacgggg tcgatcccgt ggacgcggcg
      661 gtggacctcg ttgatgtgct cggccgcggc cctggcgtct tttttcgtcc cgaacgtgat
      721 ggcgtagttg agcatcagcg tgcgctggag gcgcttccat gggctgcgct tgtagaagcc
      781 ggtctcccgg gcccccgcga tgacgagcgg gtgcgccgac tgcatcagga gcgcccgggc
      841 gccgccgaac agcacggtca gctccccatg gacctgccac gagacgctgc ccggcccgaa
      901 gaacccctgg tcgaacgaga gcacgagggc atggtacccg cgagcggtga taggctggcg
      961 ccatggagct gctgacggcc accgatgggg gggtgatctc gctccccggc ggcgagcggc
     1021 tcggccccga gcgcgtcacc ttcctccacc gcgccggcga ggtcacggtc gaggtgcgag
     1081 ggggccacga ggtggtcgtc cggtcgaacg gcgaggcccc cgggcttcgc tcggatctcc
     1141 gcgccaactg tgcgctggcc cttgccggcg gcggagccct ggtggggacg gctgaggccc
     1201 ggctgttcct cgcccgggac gggagcctcg aggccgtgga ggacttcgat cgggccgagg
     1261 ggcgcgagcg atggcacaca ccctggggcg gtccccccga cgtccgctcg ctgagcgccg
     1321 ggcccgacgg cgtcgtctac gtcaacgtcc acgtgggcgg gatcctccgc tcggagaacg
     1381 ggggcgcgac ctggtcgccc accgcgatcc cgatcgagga ggacgtccat caggtcctgg
     1441 cccatcccga gcgagccggg ctggtcctcg cggccacggc ctacgggctg gcgcgaagcg
     1501 aggacggcgg cgagagctgg gccacgatcg acgagggcct ggaccgccgc tactcccggg
     1561 cggtggcggc ggccggcgac accgtgctgc tcagcgcctc gtccgggccc ggcggaggag
     1621 aggccgcact ctaccgtctc gccggcgacc gcttcgagaa gtgcgatctc cccgatccgt
     1681 tcgggagcaa cctggacacc caccgactcg tggccgacgg ggacaccgtc gccgcggcgc
     1741 tcccgaacgg agacgtcttt ctctcggagg actcggggcg ctcatgggag aggatcgccg
     1801 acgccctccc ctccgtgcga tgtctgctgc tggagggacg gtgaggcgcc cgacgccgct
     1861 gaaccccttg atccgccgcc tcctgtggcc cgcggccctc ctgctgggcg tcacgaccta
     1921 cggcgtcgcc ggctacatgc tcatcgaggg ctggtcgttc ctcgacgccc tctacatgac
     1981 gatcatcacg ctgagcaccg tcgggttccg cgaggtccag cccctggagg cgagcggaag
     2041 ggtcttcacc ctcagcctgg tggtcctggg tgtgaccgcg gtcctcgtgg gcctcaccct
     2101 catcggcatc tggatctccg agggcaacct cggggagcga cggaggagga agcggatgga
     2161 gcagctgatc gaccggctcg aggaccactt cgtcgtctgc gccttcggtc gggtggggag
     2221 gaccgtggcc cggcgcttcc tcgacgcgga ggtgccgttc gtcgtcgtcg atcgcgacga
     2281 ggcgctcgag gcgcggatgg tcgacatgaa cgtcccccac ctgatcggag acccctccga
     2341 cgaggaggtc ctccgtcaag cggggatcga ccgcgcgcgc ggcctcgtct gcgccgtgga
     2401 ttcggacgcc acgaacgtct tcatcacgct gacggcccgg gccctcaagc ccgacctctt
     2461 catcgtggcc agggctgcga cctcggagtc gatcccgcgg ctggagaagg cgggggccga
     2521 tcgggtggtc tccccgtacg tgaccagcgg agaggccatg gcactgatgt ccctccgcca
     2581 ggacgtggtc gggatgctcg acgtggccga cctcggggac cgcgaggtgc gggtggagga
     2641 gctcgtggtg agctccgggt cgcatctcga gggcctccgg gtggacgagg cggccgggcg
     2701 ggcgctcgcc ctggcgctgc gacacgccga cggctccacc accgtggggc ccccggccga
     2761 ccggccgctg aaggcgggcg accggctcct gatcatggcc gacgagcgga cgctccaggc
     2821 ctaggagcac ggagcggggg cggcctttgg tggccgcccc cgcttggagc gcgctctccg
     2881 ccgtggctac ttgaggaccg ggaacttctt gacgagcgcg gtggactccc accaacgccc
     2941 caggccccag gtccgtcccg ccccgatgta ggccagcccc gccagcgcga tcgcgtagat
     3001 cacgtggtcg tctatgaagg ggttgttctc gggccagatc gaaccggcca cgtagaacag
     3061 gcccagccag atgatggcgc cgatcgcggc cagccgcatc ccgatcccca ggatcagcgc
     3121 caggccgatg gcagccatcg agagcatgta cacccagtcg acccaggccg agcccgcgag
     3181 cccctggaag aagtcggcga acggtccctt cgtcccgaag gcgaggaagc cctcggtcgg
     3241 cgatgccccg ttgatccacg cggcctcgcc gaagaagtcg atgacaccag tctcctcgtt
     3301 ccgccccgtg gagaacccca gcgccaacag cttgtcgagg aacgcccaca ggaacaccca
     3361 tccgaacgag atccgcagga tggcccagat gcgggttccc ttgacgggaa cctcggcttc
     3421 cgggatgtac agctccggcg ccggagccac gtcacggcgc tcgatcgtct tcatcgttct
     3481 ctcccttcat ctcgcttcgg cccagcatcg aaggcgtcac tcagtcggtc acgggccgcc
     3541 cggccccctc cggatgggtc gtttggccgt tcggctcctc ctcgatctcg agctgctcct
     3601 cgaggtcgac caccccatcc acggcccgga ccagtcggag gaggatcgcc cgggtccggg
     3661 cgctcgtcac acgaccttcc agcctcacga ccccgtcggt gacgagcgcc cggacccccc
     3721 tcgggctgat cccgagcgcc ccggagagga ccgcctcctc gacctccgcc aggatctcga
     3781 ggtcggtccg gagatacggg cgcagcaggt tcgccctcgt gcagatcccg accagacgct
     3841 ccccccgcat caccggcgcg cgcttcacgc cgtggcgctg gaggacgcgc gccgcctcgg
     3901 tcaccgtcat cccctcgacg agcgtgacga gcttcctcgt catcacgtcg gcggccacct
     3961 gaacgcggcg gcccgggtcg ccctcgtgga gaggcagcag gtccgactcg gagacgatcc
     4021 cgacgacctt cccgtcctcc acgacgggga tgccgctgat ccgcgcgccc accatgagcg
     4081 ctgccagctc cttgacgggg gtgtccgggg cggccgtcac caccccgacc gtcatcacgt
     4141 ccctcacgag catctggtcg atccccctct ctcaggctcc gcgtcgagcc cagttctcgc
     4201 tccagcgagg agccgagaag ggtccccgtg gcttgacctt gcggtcatcc caccggtagc
     4261 cgagctcgtc ctcgacgccc accacgccct cgacgcggta aaccagctcc accaggatct
     4321 cggccacgct cctccgctcc accgtcccgc ggagtcgcac gcacccccgc tcgacctcga
     4381 ccccgatcgt gtccgggtcg atccacatcg tgcggaagac gacctcctcg ctgatctcgc
     4441 gctcgatctc ctcgtcggtg cggaggaacg gctggagcag gtcgcgccgg ctgacgatgc
     4501 cgaggacctt cccctcctcg tcgatgacgg ggaggcgctt cacctgagcg tcgagcatca
     4561 gcttcgccgc ctccggcacc gtcgtctccg ggcgcaccgt cacgacgctc ggcgtcatga
     4621 tgtcgccggc ccggacctcc gcggcatccg cctcgatctc ggcgagccgg ccgggctgca
     4681 ccagccactc caggaacgag cggcggtgct tcggctcgac cttcccctcc tccagggtca
     4741 gcaggtcggc ctcggttacg atccccgcca ggagcccatc tccatcgagc accggcacac
     4801 cgctcacgcg gtgctccagc atcatgcgga cgacgtcctt gaagggcgtg tcctctcgga
     4861 cggcgacgac cgtccgcgtc atcacgtcct cgaccttcag cttcttcatc gtcacgtcac
     4921 cccgatcgcg ttccaggcct cgagggacag gaggtcccgc gaggacacca tcccgatcac
     4981 ctggcctccc tccaccaccg ggatgtgacg aaccccgagg tcgagcatcg tggccgccac
     5041 cgaggcggca tccgcctcgg gagtcaccgt caccggctcc tcggtcatat aggccgcgac
     5101 cggcgtctcc gcggggtcca cgccgtcggc gagggcgcgc gcgaggtcac gctcggtgag
     5161 gatgcccacg agcttcccgt gctcgagcac ggccagggac ccgatctcct ggtactgcat
     5221 gcggcccgcg gcgtcga
//
LOCUS       DASUTZ010000097        18773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_674430, whole genome shotgun sequence.
ACCESSION   DASUTZ010000097 DASUTZ010000000
VERSION     DASUTZ010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 18773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18773
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674430"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VF097_04250"
     CDS             complement(<1..374)
                     /locus_tag="VF097_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262039.1"
                     /translation="MIVTAAVFLVAAIVTLVIGWFQEGLAMVYVSIGASVVALAFLVA
                     GMVRRPAVAPATAGAPYGPAEGTEAAAMAPAEPRERTKPSAPLPQRRPAARPPTGRPD
                     EEKPAPEAAPSRPAAKKPAAKKP"
     gene            complement(440..1162)
                     /locus_tag="VF097_04255"
     CDS             complement(440..1162)
                     /locus_tag="VF097_04255"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEX6262040.1"
                     /translation="MMAFGRKKRQAAEDMGFSQEAVQRERVRLTPVDVQQKVFRLAFR
                     GYNEQDVDHFLDDVTEDLAALHEENKRLREELELRGPGAGTEDARRHAEEIIRRAREE
                     AARIAAETPATGAAATAAAATPAFLVREREFLQRLASLVQEHASSLKDEARRARAAAE
                     TREPEPEEIEEAQPEEIAEARPEERAEPEAEPVLDRTAPMASPGEEGDWSNPFEEPRE
                     SVDEEGSSEEEPSLRELFWGEE"
     gene            complement(1170..1442)
                     /locus_tag="VF097_04260"
     CDS             complement(1170..1442)
                     /locus_tag="VF097_04260"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX6262041.1"
                     /translation="MAVVCILLSIYWFVLFARIISSFFPPPRSPAGRTIVELLHDLTE
                     PVLRPLRNLMPPLRLGMVGLDLSPIIVFIVLGVLRSALGCRGVAFF"
     gene            complement(1471..1953)
                     /locus_tag="VF097_04265"
     CDS             complement(1471..1953)
                     /locus_tag="VF097_04265"
                     /inference="COORDINATES: protein motif:HMM:NF016363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEX6262042.1"
                     /translation="MAGVWRKTLMYLGLVEDDELEEIAPGEQTSPVQPTPRRPSRSEP
                     VVLTQQASGPESTVRTIAQPRKASAGTIHRAEPKRFNEARDLGEKFKDGVPVIMNLQQ
                     TEDSIARRLVDFASGLVFGLDGKIELVATRVYLLTPSEVEVSAEERERLRDTGFFNQY
                     "
     gene            complement(1966..2622)
                     /locus_tag="VF097_04270"
     CDS             complement(1966..2622)
                     /locus_tag="VF097_04270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX6262043.1"
                     /translation="MSGVAENVAAVRERIETACRRAGRDPSAVRLVAVTKTVDPSLVQ
                     EAAEVGVEDFGENYVQELEGKRGAAPRARWHFLGRVQSNKAHRVAKLADMVQTVEPGR
                     AWRRLASAAEDSPIPCLIQVDFTGERVGVEAAGARAFLDEVVAAGGLDAHGLMTVPPL
                     GEDPRPYFVRLRELRDELRERHPGLSELSMGMSADYEEAVVEGATMVRVGTAIFGPRP
                     "
     gene            complement(2619..3386)
                     /gene="pgeF"
                     /locus_tag="VF097_04275"
     CDS             complement(2619..3386)
                     /gene="pgeF"
                     /locus_tag="VF097_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEX6262044.1"
                     /translation="MERRALTDGLHALVPEGMESRGFLAAFTERRGGVSEPPYESLNL
                     GLHTGDRADVVAENRRLVVRTLGLERWAAGEQVHGGDVVRVDQRTASRGWDEPPIDGA
                     DALAVAQAGLSVAVLTADCLPIALASPLEGLLVAVHAGWRGLAAGIIDAALASFERPG
                     EIHAAIGPAIGPCHYEVGEDVARAVAEGSAAGAVTDRRNGRLHLDLPATAVRALRAGG
                     VRRIDPAGLCTSCEEDRFFSHRRDGITGRQALVAVRL"
     gene            complement(3494..4669)
                     /gene="ftsZ"
                     /locus_tag="VF097_04280"
     CDS             complement(3494..4669)
                     /gene="ftsZ"
                     /locus_tag="VF097_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018837710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX6262045.1"
                     /translation="MHAPQNYLAVIKVVGIGGGGVNAVNRMIEAGLKGVEFIAINTDA
                     QALLMSEADVKLDIGRETTRGLGSGSDPEIGRRAAEEHSDEIEEILKGADMVFITAGA
                     GGGTGTGGAPILARIARDLGALTIGVVTRPFSFEGKRRATQADMGIQELREHVDTLII
                     VPNDRLLQVSSEETPILDAFRLADEILYQGVEGITSLIMTPGLINLDFADVKTVMSSA
                     GSALMGIGHGAGDDRAVEAAQNAVSSPMLEASIEGARGVLLSISGGSGLTLHEVNRAA
                     EAITRAAHPEANIIFGAVIDDSLGDECRVTVIAAGFDKLGTTTTVRPRTFEVEVGGED
                     LDEEEIPVPSILRESEDEDDVFQEAGEEEQEARPARVVISADDTEADEDLDIPDFLK"
     gene            complement(4798..5523)
                     /locus_tag="VF097_04285"
     CDS             complement(4798..5523)
                     /locus_tag="VF097_04285"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEX6262046.1"
                     /translation="MTRRPNRAGIVALSTVLLGILIGTVTLRSPLFEVRRVEVRGNGR
                     VGPGQIRKLVQPAQGRNLWLVPLEDLARAVSSHPWVAQADVRRELPSTLVVEIEERIP
                     IAWVEDPGGGAAVAADGVVVQRRSESSGLVSLGAVDEIQRPGTPTAAPRASLRVAASL
                     PARIRREVAAVFRRGEGVILRMRSGARILYGRAEALGAKRATLGALLSWIDENEVPAG
                     LVDLRAPATPAIRPVSSRAEPRG"
     gene            complement(5520..6458)
                     /gene="murB"
                     /locus_tag="VF097_04290"
     CDS             complement(5520..6458)
                     /gene="murB"
                     /locus_tag="VF097_04290"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX6262047.1"
                     /translation="MPADAAREAARILTEVAGDRVRRNFPLAPLTSFRLGGPAALYLE
                     AGSQEDLAALGRALDGTGLPMLVIGKGSNLLISDEGFPGIVVRLGKGFRWAAREELRI
                     TAGGAMPLPAVANVALSHSLAGMEFGVAIPASLGGSVRMNAGAHHRSLDEVLASVEVY
                     DTRVREPRVIPASEVGFSYRDSRLPERSIVIAATAELRLDDLAAIRARMDEARRWRRE
                     TQPLAEPNCGSVFKNPAGDHAARLIDLAGAKGLRLGGARVSEKHANFFIAGEGATASD
                     VRALIEEVRRRVHDAAGVWLEPEVHLVGSFDVEGAA"
     gene            complement(6448..7872)
                     /gene="murC"
                     /locus_tag="VF097_04295"
     CDS             complement(6448..7872)
                     /gene="murC"
                     /locus_tag="VF097_04295"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009379929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX6262048.1"
                     /translation="MKDYEIRPGTIPTLEIPRLDLRRVHLVGVGGAGMSGIARLLLAR
                     GIAVSGSDLKDSPGLALLRGAGAAVTVGHRPENVGSAEAVVVSTAIGQDNPEVVAARR
                     RGLPVYARAQVLAAMMAERRGIAIAGTHGKTTTTSMTAVIFERGGLDPTYVVGGDLNE
                     SGSNARSGAGVYFIAEADESDGSFLLLDPEIAVVTNVEPDHLDFYRDEEEVEAAFAAF
                     CRRAERLIACGDDPGVRRVLEAVPIEVVTYGTGEDNDVRLEGVQTRDGEGRGVVVVDG
                     ERVDVTLSIVGRHHLLNATAAMLTARAVGVPFETAAEALRSFTGVRRRFESRGDARGA
                     RFVDDYAHHPTEITATLEAARSTGAARLVAVFQPHRYTRTEALWRELGGSLAAADLVV
                     LTDVYGAGEMPVPGVTGKLLVDSLLERAPRARVVYLPSRADVAPFLATEVRVGDLVLT
                     LGAGDVTMVADETLSRIREVTGAG"
     gene            complement(7869..8963)
                     /gene="murG"
                     /locus_tag="VF097_04300"
     CDS             complement(7869..8963)
                     /gene="murG"
                     /locus_tag="VF097_04300"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX6262049.1"
                     /translation="MRVVLAAGGTAGHIFPGLALARRLRDDHGAQVLFVGREGGQEAT
                     LVPEAGFRLATVESRPFVRRLSPAALAGPVAALRASRRAREIVRAADVAVGMGGYTSV
                     PVALAAWRERVPLVLHEQNAVPGLANRVAARWARAVALSFSEAAPRFPRRARTVVTGN
                     PVREAIARIPRDRDALAKEAREELGLEEGRRTVAVFGGSQGALHLDRAAADAARLLDG
                     SDLQLLIITGPAHLEEVEGRFPGGPIPVRAVPFVDRMELVYAAADLAVARAGAGTVAE
                     LSVAGVPALLVPYPYATGNHQEANARALQRAGGAAVLLDDQLTAEVLAERMVEMLEPT
                     RLAAMAERSASFGRPGAAGALARVVVEAAG"
     gene            complement(8967..10160)
                     /gene="ftsW"
                     /locus_tag="VF097_04305"
     CDS             complement(8967..10160)
                     /gene="ftsW"
                     /locus_tag="VF097_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX6262050.1"
                     /translation="MSTAAARKRRPHHLRLVEPGERAPRSPSRAERNDRHVTMLVVST
                     AALTLLGLIMVLSASSVEAYARFRSSFLFFNRQVVYAVVGSLAMAVTMRMHHRAWERL
                     AGPMLLVSIVLLAMVLLPGFGTVAGGSARWIELGPVTIQPSEFAKLSVISFGAALLSR
                     RWRRIQDLRQMAVPLLPVVGVVCGLILLQPDLGTAVIIVTAVFVLLVAAGARMRHLIV
                     GALATSTVGLGLIYVEGYRWSRFLSFLNPWADPQGSGYQVIQSLIAITSGGVFGVGLG
                     ASRQKWQYVPNAHTDFIYSIIGEELGLVGMLVVLGLFVVLVYAGIRVAIAAPDAFSRL
                     LAAGIVGWIGGQALINLGAVTGLLPITGVPLPFVSFGGSSLVVSLAAVGILASIARRG
                     RAERG"
     gene            complement(10157..11503)
                     /gene="murD"
                     /locus_tag="VF097_04310"
     CDS             complement(10157..11503)
                     /gene="murD"
                     /locus_tag="VF097_04310"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX6262051.1"
                     /translation="MTLDVRDRTVVVVGLAASGLAAVRVLRGEGAAVRVTEARPREEV
                     EEEVGAAEAAGAEVLAGGHAPEHLRGADLVVTSPGVPEGAPVLAWALDRGIPVWSEIE
                     LGARLSAAPYVGVTGTNGKTTTTEMVAAALRADGRDAVACGNAGHPFSLAATEGRDVL
                     AVEVSSFQLRFHQRFHPKVSVLLNVAPDHLDWHGSLEAYEAAKARIYACQGEGDLHVG
                     NADDPVAAEVSRSAPCPVAWTTLGEPGEHQLGYVGDHLVSRLDAELDLGPVRPASHAH
                     MSDAAAAAAAALGFGAAPDAVSEALRGFRPLAHRGEVVARLGDVVFVDDSKATNPHAA
                     LASIAGWDRVALIAGGRAKGVDLAPLTEAAPRLLGVVAIGEAAKEVARAFEGIRPVAR
                     ADSIEEAVGLAAAMAAPAGTVLLAPACASQDMFRDYGERGGRFAAAARALAERETG"
     gene            complement(11500..12558)
                     /gene="mraY"
                     /locus_tag="VF097_04315"
     CDS             complement(11500..12558)
                     /gene="mraY"
                     /locus_tag="VF097_04315"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX6262052.1"
                     /translation="MTAILLAAAVGLFVTLLGTPLAIRVFRFLGWGQRVREPSDPHNP
                     AHGAKIGTPTMGGLVILAGVFLGFVTTRLVTGGLSATGLLLMGVALSLGALGYVDDLL
                     KVKGHHSLGLGKTSKLLVQALVAVLFAVGLDRLTGVSTELSFIRETGIDLGIGFLIWV
                     FLMVTATSNAVNLTDGLDGLASGSVALVMGAYVIVSFWQVRHACVLLNVPGCYDISPQ
                     GSLDAAVVAAATMGAAAGFLWWNAAPARIFMGDTGSLALGGLIAAMAIATHTQLLLVI
                     LGGLFVLETASVILQVVSFRVFRRRLFRMAPIHHHFEVLGWPEFTVIVRFWIVAGLAV
                     AFGLGLFYADFLARGGLL"
     gene            complement(12555..13928)
                     /gene="murF"
                     /locus_tag="VF097_04320"
     CDS             complement(12555..13928)
                     /gene="murF"
                     /locus_tag="VF097_04320"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX6262053.1"
                     /translation="MRPRTLSSVAELTRGRLVGVDATVRGATADSRDVRPGELFVALR
                     GERTDGHRFVDAAFEAGAAGALVEEEGEYMGPVVVTLESARGLLELAADEREARDAHV
                     VAITGSTGKTSVKDLTAAVLASRHRVHASPRSFNTEVGVPVTLLRAPEDAEAIVLEMG
                     ARGRGHIALLAGVARPEVGVVTNVGAAHLEMFRSRDAVAEAKAELVEHLEPDGVAVLN
                     ADDPVVRGFEDRTAARVLLYGQDPDADVRGEDLTLDEQGRASFTLRTAEGRERVELSL
                     VGEHMASNALAAAAAGAALGLSPGECAAALKDARLSAWRMEVAETPGGVRILNDAYNA
                     NPSSMAAGLKAAVWMSRGGRCIAVLGWMAELGASSEEEHERVGELVARLGVDHLVTVG
                     EHAGRIAVGAAREGVEPGRITEVRDADEAAAEVLRIAEGGDLVFLKGSRVVGLERVAS
                     ILMEGGA"
     gene            complement(13925..15412)
                     /locus_tag="VF097_04325"
     CDS             complement(13925..15412)
                     /locus_tag="VF097_04325"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX6262054.1"
                     /translation="MLGFRRPTVASTPQLSDLLAGLPNADVRGDARTPVAGLAYQADA
                     VSPGGLFFCVPGGRHDGHDFADRALASGASVLVVERSLDLEATQVLVPSVREAMGPIA
                     AVFHGKPAERLSLVGITGTNGKTTTTYLLEGVFRAAGLTPGVIGTTGVRIDGRPVPQA
                     RTTPEAPDLHAMLARMVDEGVEAVAMEVSSHGLHQHRVGGARFSCAVFTNLSQDHLDY
                     HGTLEEYFEAKARLFTPRMSDRAAVNVDDAMGRRLLDTGLPTITYGRDEDADVRAVDE
                     EVGSGGVRFVADGLEVRSPLRGAFNVHNALAALAASRQLGIEDGEAAAGIASVTHVPG
                     RLEPVDEGQGFLVLVDYAHTPDSLDNALLAARQLAEEGGRVIAVFGCGGDRDRGKRPL
                     MGEAATRRADLTVITSDNPRTEEPIEIIREIEPGARRGGGAFVVEPDRRAAIRLALEG
                     AGEGDVVVIAGKGHETGQEFADRTIPFDDRVVAGEELRALRSDGS"
     gene            complement(15421..17106)
                     /locus_tag="VF097_04330"
     CDS             complement(15421..17106)
                     /locus_tag="VF097_04330"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX6262055.1"
                     /translation="MSRRPPAGRVRLLLAVLVLGFSSIAARVSVLHLRDAEAYEALAH
                     DQRVREVVLPARRGSIFDRNGHELAISVDARAIYANPLVIDRPWKVADRIAPLVGERR
                     RVIARALEREQPFVYLARRVDLAHAEEVEALGIAGVGLLEESKRYYPSGTLAAQVLGF
                     VGVDGLGLEGIELAYDEVLGGTPGTMVVEQDPEGRPIPQGDARIEEPVPGESLVLTID
                     RDLQWHTQRALQQAVKRNGAKGGTVVVLDPSTGDVLAMVTYPWFDANDYTEAPQEALL
                     NRAVTDVYEPGSVNKVITAAAALEEGVVGPREAIDVPGAYQVGDKLFRDVHPHGPQAM
                     TLSDIIAESSNVGTIKVAERLGRDRLATWLTRFGFGRRTGIDFPGEAEGILLDRDDWW
                     VTSMGTIPMGQGIAVTPLQMASVYGTVANDGVRVRPRLVRGTIRAGERFEPSLPGRPR
                     RVVGKDTARALREMLVRVVEEGTGDLARVEGYEVGGKTGTARKPLEDALGYSDEYVAS
                     FIGFAPADDPRIVVAAMLDEPDTVYGGVAAAPLFREVARFALADLQVPPTEPG"
     gene            complement(17103..17537)
                     /gene="ftsL"
                     /locus_tag="VF097_04335"
     CDS             complement(17103..17537)
                     /gene="ftsL"
                     /locus_tag="VF097_04335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEX6262056.1"
                     /translation="MAVAARRLEEVARRRPPVRTRRPRPAGRRAPARHARKRLPFLLF
                     SLVVVSAMVVLLVSAQAIVAQSAFRIAELRERVDRIENEHAQLRLEAARLSSPERIMR
                     AARDAGLVLPDRVEILAVSGKGASRIRSHLGTLASADLGGEG"
     gene            complement(17537..18403)
                     /gene="rsmH"
                     /locus_tag="VF097_04340"
     CDS             complement(17537..18403)
                     /gene="rsmH"
                     /locus_tag="VF097_04340"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011173150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX6262057.1"
                     /translation="MADQAHVPVLTEEIVDLMRGAETVIDMTVGAGGHAEALLEAGVA
                     RVVGVDRDPVALDLAGRHLARFGDRFVPVRARFSEVPRIADEASIRAVEGTLFDLGVS
                     SLHLDRPDRGFSYREEGPLDMRMGLEGPTAADLVNSLPEEELARIIFEHGEERASRRI
                     ARAIVRAREREAIETTAQLARIVAAAAPGRRRGPHPARRTFQALRVAVNEEVEELTAS
                     LPQAVGLLATGGRIAAISYHSIEDRILKRAFAGDERLEVLTKKPIRPSEDERARNRRA
                     RSAKLRAARRVA"
     gene            complement(18524..>18773)
                     /locus_tag="VF097_04345"
     CDS             complement(18524..>18773)
                     /locus_tag="VF097_04345"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX6262058.1"
                     /translation="ISDPKARDYARIFFGKAEQVDLDSQGRLVVPRQLRETARLGKEV
                     VIVGVRDRLEIWGKQAWEEYERQRASSYASGALVPEQL"
BASE COUNT         2698 a         7202 c         6418 g         2455 t
ORIGIN      
        1 ggcttcttgg cggcgggctt cttggcggcg ggccgcgatg gggcggcctc gggcgccggc
       61 ttctcctcgt cgggccgccc ggtcggcggc cgcgcggcgg ggcgccgctg gggcaggggc
      121 gcgctcggct tggtccgttc gcgaggctca gccggcgcca tggccgcggc ttccgtcccc
      181 tccgccgggc cgtaaggggc gccggccgtg gccggcgcaa cggcgggcct cctcaccatc
      241 cccgccacga ggaaggcgag cgcgaccacg ctcgccccga tcgagacgta caccatggcc
      301 agcccctcct ggaaccagcc gatgaccagc gtgacgatcg ctgcgaccag gaacacggcg
      361 gcggtgacga tcacgtggtc tcctgcgggt cggtcacgcg cgtagcgtat cggagcgaac
      421 cgagggagtc atccagccct cactcctcgc cccagaagag ctcgcgcagc gagggctcct
      481 cctcggagct tccctcctcg tcgacggatt ccctgggctc ctcgaagggg ttggaccagt
      541 ccccctcctc gcccggcgag gccatcgggg ccgtgcgatc caggacgggc tccgcctcgg
      601 gctcggcgcg ctcttcaggc cgggcctccg cgatctcctc cggctgggcc tcctcgatct
      661 cctcaggctc gggctcccgg gtctcggcgg ccgcccgggc tcgccgcgcc tcgtccttga
      721 ggctcgaggc gtgttcctga accagcgatg ccagtcgctg caggaactcg cgctcacgga
      781 cgaggaacgc gggggtcgcg gcggccgcgg tggccgcggc gccggtggcg ggggtctcgg
      841 ccgcgatccg cgccgcctcc tcccgggccc ggcggatgat ctcctcggcg tgccggcgag
      901 cgtcctccgt gccggcgccc ggcccccgga gctcgagctc ctcgcggagc cgcttgttct
      961 cctcgtgcag ggcggccaga tcctcggtga cgtcgtcgag gaaatggtcc acgtcctgct
     1021 cgttgtagcc gcggaacgcg agcctgaaca ccttctgctg gacgtccacg ggcgtgagcc
     1081 tgacgcgctc ccgctggacc gcctcctggg agaagcccat gtcttccgcg gcctgcctct
     1141 tcttccgtcc gaacgccatc atcgcctcct cagaagaacg cgacccctcg acagcccaac
     1201 gcggagcgaa ggaccccgag cacgatgaac acgatgatcg gcgagaggtc gaggcccacc
     1261 ataccgagtc tgagcggcgg catcaggttc cggagcgggc gcagcacggg ctcggtgaga
     1321 tcgtggagga gctcgacgat cgtgcggccg gcgggcgagc gcggaggagg gaagaacgag
     1381 gagatgattc gcgcgaagag cacgaaccag tagatcgaaa gcaggatgca gacgactgcc
     1441 aatcaatccc ccctcacggc gaacgccgcc tcagtactgg ttgaagaacc ccgtgtcccg
     1501 gaggcgctcc cgctcctcgg ccgacacctc cacctccgac ggcgtcagca ggtacacacg
     1561 ggtggccacg agctcgatct tcccgtcgag cccgaagacc agccccgagg cgaagtcgac
     1621 caggcgacgg gcgatcgagt cctccgtctg ctggaggttc atgatcaccg gtacaccgtc
     1681 cttgaacttc tccccgaggt cgcgcgcctc gttgaaccgc ttgggctcgg cccgatggat
     1741 cgtcccggcc gaggccttcc tcggctgggc gatcgtacgg accgtgctct ccggcccgga
     1801 cgcctgctgc gtcagcacga ccggctcgga gcgcgacggg cgccgaggcg tcggctgcac
     1861 cggcgaggtc tgctcgccgg gagcgatctc ctcgagctcg tcgtcctcga cgagccccag
     1921 gtacatcagg gtcttgcgcc acacgcctgc catccgtcta cctcctcatg gtcgggggcc
     1981 gaagatcgcc gtcccgacgc gcaccatggt agcgccttcg accaccgctt cctcgtagtc
     2041 ggcggacatc cccatcgaga gctccgagag ccccgggtgc cgctctcgaa gctcgtcgcg
     2101 aagctcccgc agccgcacga agtagggtcg cggatcctcc cccagcggcg ggaccgtcat
     2161 cagtccgtgg gcgtcgaggc cgccggcggc gaccacctcg tcgaggaagg cacgcgcgcc
     2221 ggcggcctcg acgcccaccc gctcgcccgt gaagtccacc tggatcaggc acgggatcgg
     2281 ggagtcctcg gccgccgatg ccagtcggcg ccacgcgcgt ccgggctcaa ccgtctgcac
     2341 catgtcggcg agcttcgcca cccgatgggc cttgttgctc tgcaccctcc ccaggaagtg
     2401 ccaccgtgct cggggtgcgg ccccccgctt gccctccagc tcctgcacgt agttctcgcc
     2461 gaagtcctcg acccccacct cagccgcctc ctggacgagc gacggatcga ccgtcttcgt
     2521 cacagcaacc aggcgcaccg ccgacggatc cctccccgcg cgacggcagg ccgtctcgat
     2581 ccgctcacgg accgccgcca cgttctcggc caccccgctc acagccgcac cgccaccagc
     2641 gcctgccgcc ccgtgatccc gtcccgccga tgggagaaga accgatcctc ctcgcaggac
     2701 gtgcagaggc cggcggggtc gatcctccgg acgcctcccg cgcggagcgc gcgcacggcg
     2761 gtggcgggga gatcgaggtg caggcgaccg ttccggcggt ccgtgacggc ccccgccgcc
     2821 gatccctccg cgacggcacg ggccacgtcc tcgcccacct cgtagtggca cgggccgatg
     2881 gccggcccga tcgccgcgtg gatctctccc ggtcgttcga agctcgccaa ggccgcgtcg
     2941 atgatcccgg cggccaggcc ccgccacccc gcgtggaccg cgaccagcag cccctcgagg
     3001 ggagaggcca gcgcgatggg gaggcagtcc gcggtgagca ccgccaccga cagcccggcc
     3061 tgggcgacgg ccagcgcgtc ggccccgtcg atcggcggct cgtcccagcc ccgggaggcc
     3121 gtccgctgat ccacgcgaac gacgtcaccg ccgtggacct gctcccccgc cgcccaccgc
     3181 tcgagcccca gcgtccgtac caccaggcgc cggttctcgg cgacgacgtc ggcccggtcc
     3241 cccgtgtgca gcccgaggtt caggctctcg tacggcggct cgctgacgcc gcctcgacgc
     3301 tcggtgaagg ccgccaggaa cccgcgcgat tccatgccct ccgggacgag ggcgtggagg
     3361 ccgtcggtca gcgcgcgtcg ctccatcgag gggatgggtg ggggcgggga gggccacacc
     3421 gtcaaggcgg gggaagggcg cctcgttcgg gccctccccg cccccacacc tcttggggga
     3481 ccgttccggc cccctacttc aggaagtccg ggatgtcgag gtcctcgtcg gcctcggtgt
     3541 cgtcggcgct gatcaccacg cgggccggac gggcctcctg ctcctcctct ccggcttcct
     3601 ggaacacatc gtcctcgtcc tccgactccc gcaggatcga ggggacgggg atctcttcct
     3661 cgtcgagatc ctcgccgccc acctcgacct cgaaggtccg ggggcgaacg gtcgtcgtcg
     3721 tccccagctt gtcgaagccc gccgcgatca ccgtgacgcg gcactcgtcg cccagcgagt
     3781 cgtcgatcac cgcgccgaag atgatgttcg cctcggggtg ggcggccctc gtgatcgcct
     3841 cggctgcccg gttgacctcg tggagggtga ggcccgagcc gcccgagatc gagagcagca
     3901 ccccccgagc cccctcgatc gaggcctcga gcatcgggga cgagacggcg ttctgcgctg
     3961 cctccaccgc gcgatcgtcg ccggcaccgt gcccgatccc catcagggcc gagccggcgc
     4021 tcgacatcac ggtcttcacg tccgcgaagt cgaggttgat gaggccgggg gtcatgatca
     4081 ggctcgtgat gccctccacc ccctgataga ggatctcgtc ggccagccgg aaggcgtcga
     4141 gtatcggggt ctcctcgctc gagacctgga ggagacggtc gttcgggacg atgatcagcg
     4201 tgtccacgtg ctcccgcagc tcctggatcc ccatgtcggc ctgcgtcgcc cgccgcttcc
     4261 cctcgaacga gaagggccgg gtcacgacac cgatcgtcag ggcccccagg tcgcgggcga
     4321 tccgggccag gatcggggcg ccgccggtgc cggtgccgcc gcccgcgccg gcggtgatga
     4381 acaccatgtc ggcgcctttc aggatttcct cgatctcgtc ggagtgctcc tcggcggccc
     4441 gccggccgat ctccgggtcc gaccccgagc ccagcccgcg ggtcgtctcc cggccgatgt
     4501 ccaacttcac gtcggcctcg gacatcagga gcgcctgcgc gtcggtgttg atcgcgatga
     4561 actcgacccc cttgagaccg gcctcgatca tccgattcac ggcgttgacg ccgccaccgc
     4621 cgatcccgac gaccttgatg accgccaggt agttctgtgg agcgtgcatc gcgttcatcc
     4681 gctgcctccc cggtagcctc agcgccccca cgctcaagct tcaacctaaa ctcgaggttg
     4741 aatgttatgt aaacctgccc tacagggcga ggaatctaca ccatcgtgtc ggggacgtca
     4801 accccggggt tcggctcggg aggagaccgg ccggatggcc ggggtcgcgg gcgcacggag
     4861 gtccacgagg ccggcaggga cctcgttctc gtcgatccag ctcaggaggg cccccagcgt
     4921 ggcccgcttc gcgcccaggg cctccgccct cccgtagagg atcctcgccc ccgacctcat
     4981 ccggaggatg accccctcgc cgcggcggaa gacggcggcc acctcgcgac ggatccgagc
     5041 aggcagtgag gccgcgactc tgagcgaggc ccgcggggcg gccgtcggcg tccccggacg
     5101 ctggatctca tccacggctc cgagcgagac gaggccagag gactccgacc gccgctggac
     5161 cacaaccccg tccgctgcca cggcggctcc cccgccggga tcctcgaccc aggcgatcgg
     5221 gatgcgctcc tcgatctcga ccacaagcgt cgacgggagc tcgcgacgga cgtcggcctg
     5281 ggccacccac ggatgggacg agaccgcccg cgccagatcc tccaacggca cgagccagag
     5341 gttcctcccc tgggctggct ggacgagctt ccggatctga ccgggcccca cccttccgtt
     5401 cccacgcacc tcgacccgac ggacctcgaa gagcggcgag cggagggtga ccgtcccgat
     5461 caggatcccc agcagcaccg tggagagggc gacgatcccg gcgcgattcg gtcttcgggt
     5521 cacgcggctc cctccacgtc gaacgatccg acgagatgga cctccggctc gagccacacc
     5581 cccgccgcgt cgtgcacgcg tcggcgcacc tcctcgatca gggcgcggac gtccgaggcg
     5641 gtcgctccct cgcccgcgat gaagaagttc gcgtgcttct ccgacacgcg cgccccgccg
     5701 agccgcaagc ccttggcccc ggccaggtcg atgaggcgcg cagcgtggtc acccgcgggg
     5761 ttcttgaaga cgctcccgca gttcggctcg gcgaggggct gcgtctcacg ccgccacctc
     5821 cgagcctcgt ccatccgggc ccggatcgcg gcgaggtcgt ccagccggag ctcggccgtc
     5881 gccgcgatca cgatgctccg ctcggggagg cgcgaatcgc gataggagaa tcccacctcg
     5941 ctcgccggga tgacgcgcgg ctcccgaacg cgcgtgtcgt agacctcgac cgacgcgagg
     6001 acctcatcga gcgaccggtg atgggcccct gcgttcatac ggaccgagcc ccccagcgac
     6061 gcggggatcg cgacgccgaa ctccatcccc gcgagcgagt gcgacagggc gacgttcgcg
     6121 actgccggga ggggcatcgc tcctccggcc gtgatccgga gctcctccct cgccgcccag
     6181 cggaagccct tgcccaggcg caccacgatc cccgggaagc cctcgtcgga gatcagcagg
     6241 ttcgagccct tcccgatcac gagcatgggg agccccgtcc cgtccagcgc tcgccccagc
     6301 gccgccaggt cctcctgcga ccccgcctcc aggtacaggg cggcgggacc accgagccgg
     6361 aaggacgtca gcggggccag cgggaagttc cgccggaccc ggtccccggc cacctcggtc
     6421 aggatccggg ccgcctcgcg cgcggcgtca gccggcaccg gtcacctccc ggatcctgga
     6481 gagcgtctcg tccgccacca tcgtgacgtc gccggctccc agcgtgagga cgaggtcccc
     6541 gacccggacc tcggtggcca ggaacggcgc cacgtcggcc cgcgacggca ggtaaacgac
     6601 ccgggcccgg ggcgcccgct ccagcagcga atcgaccagg agcttcccgg tcacccccgg
     6661 gacgggcatc tcccccgcgc cgtacacgtc ggtgagaacc acgaggtccg cggcggccag
     6721 gctgccgccg agctcgcgcc agagcgcctc ggtcctcgtg tagcgatggg gctggaagac
     6781 ggccacgagt cgggccgcgc cggtggacct ggccgcctcg agggtcgccg tgatctccgt
     6841 cggatggtga gcgtagtcgt cgacgaatcg tgccccgcgc gcgtcgcccc gcgactcgaa
     6901 ccggcgccgt acgcccgtga agctgcggag cgcctcggcc gcggtctcga acggcacccc
     6961 cacggcccga gcggtcagca tcgctgcggt cgcgttcagg aggtggtggc ggccgacgat
     7021 ggagagcgtc acgtcgacgc gctccccgtc gacgaccacg acgcctcgcc cttcgccatc
     7081 cctcgtctgg acgccctcca ggcggacgtc gttgtcctcg cccgtgccgt aagtgacgac
     7141 ctcgatcggc accgcctcca ggaccctccg gacccccgga tcgtccccgc aggcgatgag
     7201 ccgttcggcc cgacggcaga aggccgcgaa cgccgcttcg acctcctcct cgtcccggta
     7261 gaagtcgagg tgatccggct ccacgttcgt caccaccgcg atctcgggat cgaggaggag
     7321 gaaggaaccg tcggactcgt cggcctcggc gatgaagtaa acgccagcgc cggagcgggc
     7381 gttgctcccg ctctcgttca ggtctccgcc cacgacgtat gtggggtcga gcccgccgcg
     7441 ctcgaagatc acggccgtca tcgaggtcgt cgtcgtcttc ccatgggtcc cggcgatcgc
     7501 gatcccccgg cgctccgcca tcatggcggc gagcacctgg gcccgtgcgt agacgggaag
     7561 cccccgccgg cgagccgcca cgacctcggg gttgtcctgg ccgatcgccg tcgagaccac
     7621 caccgcctcg gccgagccga cgttctcggg gcgatgcccc acggtcaccg ccgccccagc
     7681 gccgcgcagg agcgcgagtc caggggagtc cttgaggtcg gagcccgaca ccgcgatccc
     7741 tcgcgccagc agcagcctgg cgatcccgct catccccgcg ccgcccacgc ccacgaggtg
     7801 gacgcggcgc aggtcgagcc gggggatctc cagcgtgggg atcgtgcccg gccggatctc
     7861 gtaatccttc atccggcggc ctccacgacc acccgggcca gagcccccgc cgcaccgggc
     7921 ctcccgaacg acgccgagcg ctcggccatc gccgcgagcc gggtcggctc gagcatctcg
     7981 accatccgct ctgcgagcac ctcggcggtc agctggtcgt cgagcaggac cgccgctccc
     8041 cccgcccgct gcagcgcccg ggcgttcgcc tcctgatggt tgccggtggc gtaggggtac
     8101 gggaccagca gggccggcac tccggcgacg ctcagctcgg cgacggtgcc cgccccggca
     8161 cgggctacgg ccaggtcggc cgcggcatac acgagctcca tgcggtccac gaacggcacg
     8221 gcgcgcaccg gaatcggccc tccggggaac cgcccctcca cctcctcgag gtgggccggc
     8281 ccggtgatga tcaggagctg gagatcggag ccgtccagca gacgcgcggc gtcggcggcc
     8341 gcccgatcca ggtggagggc cccctggctc ccgccgaaga cggcgaccgt ccggcggccc
     8401 tcctccaggc cgagctcctc tcgggcctcc ttcgccagcg cgtcgcgatc ccgggggatc
     8461 cgggcgatcg cctcgcggac cgggttcccc gtgaccaccg tgcgggctct gcgcgggaac
     8521 cgaggcgcgg cctccgagaa cgaaagcgcg accgccctcg cccaccgggc ggccacgcgg
     8581 ttcgccaggc cgggcacggc gttctgctcg tggaggacga ggggcacgcg ctctcgccag
     8641 gcggccagcg cgaccgggac cgacgtgtac ccgcccatcc ccacggcgac gtcggcggct
     8701 cggacgatct cccgagcccg ccgcgaggcg cgcagcgcgg ccaccggccc cgcgagggcg
     8761 gccggcgaga gtctccgcac gaacggtcga gactccaccg tggccaggcg gaagccggcc
     8821 tcgggaacga gggtcgcctc ctggcctccc tcccgcccta cgaagagcac ctgcgcaccg
     8881 tggtcgtccc gtagccgacg cgccagcgcg agcccgggga agatgtgccc ggccgtcccc
     8941 ccggccgcca ggaccacccg catccctcag cccctctccg cccggcctcg ccgcgcgatg
     9001 gacgcgagga tgccgacggc cgccagcgag acgacgaggg acgatccgcc gaaggagacg
     9061 aacggcagcg gaaccccggt gatcggcagg agcccggtca ctgcccccag gttgatgagg
     9121 gcctggcctc cgatccaccc cacgatcccc gcagccagca ggcgcgagaa ggcgtccggc
     9181 gcggcgatcg ccacgcggat ccccgcgtag acgaggacga cgaagagccc caggacgacg
     9241 agcatcccca ccagcccgag ctcctccccg atgatcgagt agatgaagtc ggtgtgagcg
     9301 ttcgggacgt actgccactt ctggcgagag gcgcccagcc ccaccccgaa cacgcccccc
     9361 gaggtgatcg cgatcagcga ctggatcact tggtacccgc tgccctgggg atcggcccac
     9421 gggttgagga acgagaggaa tcgcgaccag cgatatccct ccacgtagat caggccgagc
     9481 ccgaccgtgc tcgtggcgag cgcccccacg atcaggtggc gcatccgggc ccccgccgcc
     9541 accaacagga cgaacaccgc ggtcacgatg atgacggccg tccccagatc gggctggagg
     9601 aggatcagcc cgcacacgac cccgacgacc gggagcaggg gcaccgccat ctggcgaaga
     9661 tcctggatcc ggcgccaccg gcgcgacagc agggcggccc cgaacgagat cacggagagc
     9721 ttggcgaact cggacggctg gatcgtcacc ggcccgagct cgatccagcg agccgagccg
     9781 ccggccacgg ttccgaaccc cggcaggagg accatggcca ggagcacgat cgacacgagc
     9841 agcatcgggc ccgccagccg ctcccaggcc cgatggtgca tccgcatcgt cacggccatg
     9901 gcgagcgaac ccaccaccgc gtagacgacc tgtcggttga agaagaggaa gctcgacctg
     9961 aaccgcgcgt acgcctccac cgaggaggcc gagagcacca tgatcaggcc gaggagggtg
    10021 agcgcggccg tgctcaccac gagcatcgtg acgtgccggt cattgcgctc ggcccgggaa
    10081 gggctgcgag gggcccgctc ccccggctcg acgagccgga ggtgatgggg gcgacgcttc
    10141 cgggccgcgg cggtgctcat cccgtctccc tctcggcgag cgcccgggcg gcggcggcga
    10201 accgaccccc gcgctctccg tagtccctga acatgtcctg gctggcgcag gccggggcga
    10261 gcaggacggt cccggcgggc gccgccatcg cggccgcgag ccccaccgcc tcctcgatcg
    10321 agtcagcgcg ggccacgggg cggatcccct cgaacgctcg ggccacctcc tttgcggcct
    10381 cgccgatcgc cacgaccccg aggaggcgcg gagccgcctc cgtcagcggc gcgaggtcca
    10441 cgcccttggc tcgccctccg gcgatcaggg ccacccggtc ccaccccgcg atcgaggcca
    10501 gggccgcgtg ggggttcgtg gccttcgagt cgtccacgaa gacgacatca ccgaggcggg
    10561 ccaccacctc gccgcggtgg gccagcggac ggaagccccg gagggcctcg ctcacggcgt
    10621 ccggcgccgc cccgaacccg agcgccgccg cggccgccgc ggccgcgtcg gacatgtgcg
    10681 catggctcgc cggacggacg ggcccgaggt cgagctcggc gtccagccgg gacacgaggt
    10741 ggtcgcccac gtagcccagc tgatgctcgc ccggctcgcc cagcgtcgtc caggccaccg
    10801 ggcagggggc ggaccgagag acctcggcgg cgacggggtc gtccgcgttc ccgacgtgca
    10861 ggtccccctc gccctgacag gcgtagatcc gggccttcgc cgcctcgtag gcctccaggc
    10921 tcccgtgcca gtcgaggtga tcgggggcga cgttgagcag caccgagacc ttgggatgga
    10981 agcgctggtg gaagcggagc tgaaaggagg acacctccac cgccaggaca tcgcggccct
    11041 cggtcgcggc tagggagaag ggatggccgg cgttcccgca cgccaccgcg tcccgaccgt
    11101 cggcgcgaag cgccgcggcc accatctcgg tggtggtggt cttcccgttc gtgcccgtca
    11161 cgcccacgta cggggcggcg gacagccgag cccccagctc gatctcgctc cacacgggga
    11221 tcccgcgatc gagggcccat gccaggacgg gagctccctc gggcacgcca gggctcgtca
    11281 cgacgaggtc ggcgccgcgg aggtgctcgg gcgcgtggcc gcccgcgagg acctccgctc
    11341 ccgcggcctc cgcggcgccg acctcctcct cgacctcctc gcgcgggcga gcctcggtca
    11401 cccggaccgc cgccccctcg cctcggagca cccggaccgc cgccaggccg ctcgccgcca
    11461 ggcccaccac gacgaccgtc cggtcccgca cgtcgagcgt cacagcaggc ctccccgggc
    11521 caggaagtcg gcgtagaaga gccccagccc gaaagcgacc gccaggcccg ccacgatcca
    11581 gaaccggacg atgacggtga actcgggcca gcccaagacc tcgaagtggt gatggatcgg
    11641 cgccatgcgg aacagccgcc gtcggaagac ccggaacgag acgacctgca ggatcaccga
    11701 ggccgtctcc aggacgaaga gcccgcccag gatcacgagg aggagctggg tgtgggtcgc
    11761 gatcgccatc gcggcgatca ggcccccgag ggcgagcgat cccgtatctc ccatgaagat
    11821 ccgggcgggg gccgcgttcc accacaggaa gccggccgcg gctcccatcg tggccgcggc
    11881 caccaccgcg gcgtcgagcg agccctgggg ggagatgtcg tagcagcccg gaacgttgag
    11941 caggacgcat gcgtgtcgga cctgccagaa cgagacgatc acgtaggctc ccatgaccag
    12001 cgccaccgac cccgacgcca gcccgtccag accgtcggtg aggttcacgg cgttggaggt
    12061 cgccgtcacc atcaggaaga cccagatcag gaagccgatc ccgaggtcga tccccgtctc
    12121 ccggatgaac gagagctcgg tggacacgcc ggtgagccgg tccaggccga ccgcgaagag
    12181 cacggccacg agcgcctgga ccaacagctt ggaggtcttc cccaggccca gcgagtggtg
    12241 gcccttcacc ttcaggaggt cgtcgacgta ccccagggcg ccgagcgaga gggccacgcc
    12301 catcaggagg aggccggtgg cgctcagccc cccggtcacg agcctcgtgg tgacgaaccc
    12361 gaggaacacg cccgcgagga tcaccagccc gcccatcgtg ggcgtcccga tcttggcccc
    12421 gtgcgccgga ttgtgtgggt cgctcggctc tcgcacccgc tggccccatc ccaggaagcg
    12481 gaagacgcgg atcgcgagcg gggtccccag cagggtgacg aacagcccca ccgccgcggc
    12541 caggaggatc gcggtcacgc gcccccctcc atcaggatgg aggcgacgcg ctccaggccg
    12601 accacccggg agcccttcag gaacaccagg tcgcctccct cggcgatccg gaggacctcg
    12661 gccgccgcct cgtccgcgtc acggacctcg gtgatgcggc ccggctccac gccctccctg
    12721 gccgccccga cggcgatcct ccccgcatgc tcgcccaccg tgacgaggtg atccacaccg
    12781 aggcgggcca cgagctctcc cacccgctcg tgctcctcct cggaggacgc gccgagctcg
    12841 gccatccagc cgaggaccgc gatgcaccgc ccgcctcgag acatccagac ggccgccttc
    12901 aggcccgcgg ccatggacga ggggttggcg ttgtacgcgt cgttgaggat ccgcacgccg
    12961 ccgggggtct cggccacctc catccgccac gccgacagcc tggcgtcctt gagggcggcg
    13021 gcgcactccc ccggcgagag ccccagcgcc gcccccgccg cggccgcggc cagcgcgttc
    13081 gacgccatgt gctcgcccac gagcgacagc tccacgcgct cgcggccctc cgcggtacgc
    13141 agggtgaacg acgcccgccc ctgctcgtcg agcgtcaggt cctccccccg gacgtcggca
    13201 tcgggatcct gcccgtacag gaggacgcgg gccgcggttc ggtcctcgaa gccgcggacc
    13261 acggggtcgt cggcgttcag gacggccacg ccgtccggct cgaggtgctc cacgagctcg
    13321 gccttggcct cggctacggc gtcgcggctc cggaacatct cgaggtgcgc cgccccgacg
    13381 ttcgtgacga cgcccacctc gggacgggcc acgccggcca ggagcgcgat gtgtccccgt
    13441 ccccgcgcgc ccatctccag gacgatggcc tcggcatcct cgggggcccg cagcagcgtg
    13501 accggaaccc ccacctcggt gttgaaggag cgcgggctcg cgtgcacccg gtgccgggac
    13561 gccagtacgg ccgccgtgag gtccttgacc gaggtcttgc cggtcgagcc cgtgatcgcc
    13621 acgacgtgcg cgtcccgggc ctcccgctcg tcggcggcga gctcgaggag ccccctcgcc
    13681 gactcgagcg tgacgacgac ggggcccatg tactcgccct cctcctccac cagggccccg
    13741 gccgcgccgg cctcgaaggc cgcgtccacg aagcggtgcc cgtcggttcg ctcgccgcgg
    13801 agagcgacga agagctcgcc gggccggacg tcgcggctgt cggccgtggc cccccggacg
    13861 gtggcgtcca cgccgacgag ccgccccctc gtgagctcgg ccaccgagga gagggtccgt
    13921 ggcctcacga cccatcactc ctcagggccc gaagctcctc cccggcgacc acccggtcgt
    13981 cgaaggggat cgtgcgatcg gcgaactcct gccctgtctc gtggcccttc ccggcgatga
    14041 cgacgacgtc tccctcgccg gccccctcca gcgccaggcg gatcgccgcg cgccggtcgg
    14101 gctccacgac gaaggcgccg cccccgcgcc gcgcgccggg ctcgatctcc cggatgatct
    14161 cgataggctc ctccgtcctg gggttgtcgc tggtgatcac ggtcaggtcc gcccgccggg
    14221 tggcggcctc gcccatcagc gggcgcttcc cccggtcccg gtccccaccg cagccgaaca
    14281 ccgcgatgac gcggccgccc tcctccgcca gctgccgcgc cgcgaggaga gcgttgtcga
    14341 gactgtcggg ggtgtgcgcg tagtccacga gcaccaggaa gccctgcccc tcgtccacgg
    14401 gctcgaggcg gccgggcaca tgggtgacgg aggcgatccc cgccgcggcc tccccgtctt
    14461 cgatcccgag ctgccgggag gccgccagcg ccgcgagggc gttgtggacg ttgaaggccc
    14521 cccggagcgg cgagcgcacc tcgaggccgt ccgccacgaa ccgcacgccg ccggacccga
    14581 cctcctcgtc cacggcccgc acgtcggcat cctcgtcccg tccgtaggtg atcgtgggaa
    14641 gcccggtgtc gagcaggcgc cggcccatgg cgtcgtcgac gttcactgcc gcccggtcgc
    14701 tcatgcgggg cgtgaacagc cgagccttcg cctcgaagta ctcttcgagg gtcccgtgat
    14761 agtcaagatg gtcctgggac aggttcgtga acacggcgca ggagaaccgc gcgccgccca
    14821 cccggtgctg gtggagaccg tgagatgaga cctccatggc cacggcctcg accccttcgt
    14881 ccaccatccg ggccagcatc gcgtggaggt cgggggcctc cggggtggtc ctggcctggg
    14941 ggacgggtcg gccgtcgatc cgcacgccgg tggtcccgat caccccgggg gtcaggcccg
    15001 cggcgcggaa cacgccctcc agcaggtacg tcgtggtcgt cttcccgttc gtgccggtga
    15061 tccccacgag cgagagccgc tcggcgggct tcccgtggaa gaccgcagcg atcgggccca
    15121 tcgcctccct gaccgagggg accaggacct gggtcgcctc gaggtcgagg gatcgctcca
    15181 ccaccaggac cgaggctccg ctcgcgagcg cccgatcggc gaagtcgtgc ccgtcgtgcc
    15241 gcccgccggg gacgcagaag aagaggccgc cgggagagac cgcatcggcc tggtacgcca
    15301 gcccggcgac gggggtcctc gcatcgccgc gcacgtccgc gttgggaagc ccggcgagga
    15361 ggtcggagag ctggggcgtc gatgccaccg tgggccgacg aaatcctagc accggggcca
    15421 tcatcccggc tccgtgggag ggacctggag gtccgccaga gcgaaccgcg ccacctcgcg
    15481 gaagagcggg gctgcggcca cgccgccgta gaccgtgtcg ggctcgtcca gcatcgcggc
    15541 gaccacgatc ctggggtcgt cggccggggc gaagccgatg aacgaggcga cgtactcgtc
    15601 gctgtagccg agggcgtcct cgaggggctt gcgggccgtt cccgtcttgc cgcccacctc
    15661 gtacccctcg acgcgggcca gatcgccggt accttcctcg accacccgga cgagcatctc
    15721 tcggagggcg cgggccgtgt ccttcccgac cacgcgacgg gggcgcccgg gaagggacgg
    15781 ttcgaaccgc tcgcccgcgc ggatcgtccc ccggaccagg cgggggcgga cccgcacgcc
    15841 gtcgttggca accgtgccgt agaccgacgc catctggagc ggcgtgacgg cgatcccctg
    15901 ccccatcggg atcgtcccca tgctcgtcac ccaccagtcg tcgcgatcga gcaggatgcc
    15961 ctcggcctcg cctgggaagt cgatcccggt cctccgcccg aagccgaacc tcgtcagcca
    16021 ggtcgccaga cgatcccggc caagccgctc ggccaccttg atcgtcccca cgttcgacga
    16081 ctccgcgatg atgtccgaga gcgtcatcgc ctgtgggccg tgcgggtgga cgtcccggaa
    16141 cagcttgtcc ccgacctgat acgcgccggg cacgtcgatc gcctcgcggg gcccgacgac
    16201 accctcctcc agcgccgccg cggcggtgat caccttgttc accgagcccg gctcgtagac
    16261 gtcggtgacg gcgcggttga gcaacgcctc ctggggggcc tcggtgtagt cgttggcgtc
    16321 gaaccacgga tacgtcacca tcgccagcac atcgccggtc gagggatcga ggaccacaac
    16381 ggtgccgccc ttcgccccgt tgcgcttgac cgcctgctgg agggcacgct gcgtatgcca
    16441 ctggaggtcg cggtcgatcg taaggacgag agactcgccg gggaccggct cctcgatcct
    16501 ggcgtccccc tgcggtatcg gtcgcccctc ggggtcctgc tcgacgacca tcgtgcccgg
    16561 agtgccgccc aggacctcgt cgtaggccag ctctatgccc tcgaggccga ggccgtccac
    16621 gccgacgaag ccgagcacct gcgcggctag ggtccccgaa gggtagtaac gcttgctctc
    16681 ttcgaggagc cccaccccag cgatccccag cgcctccacc tcctcggcgt gggcgagatc
    16741 cacgcgccgg gccaggtaca cgaagggctg ctcgcgctcc agggcccggg cgatgactcg
    16801 gcgacgctcg cccaccaagg gggcgatgcg atcggccacc ttccaaggcc ggtcgatcac
    16861 cagggggttc gcgtagatcg ccctggcgtc caccgagatg gcgagctcgt gcccgttccg
    16921 gtcgaagatc gacccgcgcc gagccggcag gaccacctcg cggacccgct ggtcgtgggc
    16981 gagggcctcg taggcctcgg cgtctcgcag gtgaaggacg gagacccgcg ccgcgatgct
    17041 cgagaagccg agcaccagaa ccgccagcag gagtcggacg cggccggccg gcgggcgccg
    17101 gctcatccct cccctccgag atccgcgctg gccagggttc ccaggtgcga gcggatcctg
    17161 ctcgccccct tcccagacac ggcgaggatc tcgacgcggt ccggaaggac gagccccgcg
    17221 tcgcgggccg cccgcatgat gcgctcgggc gacgagagcc gggccgcttc gagccggagc
    17281 tgcgcgtgct cgttctcgat ccggtccacc cgctcccgga gctcggcgat gcggaaggcc
    17341 gactgggcca cgatggcctg ggcgctgacg aggagaacga ccatcgcgga gacgacgacg
    17401 agggagaaga ggaggaacgg gaggcgcttg cgggcgtggc gcgccggcgc ccgccgcccg
    17461 gccgggcggg gccggcgggt cctcacgggc gggcgccggc gcgccacctc ctcgaggcgc
    17521 ctcgcggcga cggccatcag gccaccctcc gcgcggcgcg cagtttcgcg ctcctcgccc
    17581 tgcggttgcg cgcccgctcg tcctcgctcg gacggatcgg cttcttggtc agcacctcga
    17641 gccgctcgtc cccggcgaag gcgcgcttga ggatccggtc ctcgatcgag tggtaggaga
    17701 tcgccgcgat ccggcctccg gtggcgagga ggcccaccgc ctggggaagg gaggcggtga
    17761 gctcctcgac ctcctcgttc accgcgacgc gaagcgcctg gaaggtccgg cgggcggggt
    17821 gcggcccccg ccggcgtccg ggcgccgccg cggccacgat ccgggcgagc tgcgcggtgg
    17881 tctcgatcgc ctcgcgctcc cgggcgcgga cgatcgctcg ggcgatgcgg cgcgacgcgc
    17941 gctcctcacc gtgctcgaag atgatccggg ccagctcctc ctcggggagc gagttcacga
    18001 ggtcggccgc cgtgggtccc tcaagcccca tccgcatgtc cagcggcccc tcctcgcggt
    18061 aggagaagcc ccgatccggg cggtcgaggt ggagggagga gacaccgaga tcgaacaggg
    18121 tgccttcgac cgcccggatc gaggcttcgt ccgcgatccg gggaacctcc gagaagcggg
    18181 cgcgcacggg gacgaaccgg tcgccgaacc gggccaggtg ccggccggcc agatccaggg
    18241 ccacggggtc gcgatcgacg cccaccaccc gggccacgcc ggcctcgagc agggcctcgg
    18301 cgtgcccgcc agcccccacg gtcatgtcga tcacggtctc cgctccgcgc atcaggtcga
    18361 cgatctcctc ggtcaggacg gggacgtgag cctggtcagc caagcgtcca tacggccagg
    18421 aggccgacgc cgagcgtggc cccgctcatc agcgccgtca ggcccagcag gaacagcgac
    18481 tgacgcacct cgcgatccag ctcgctcatg accctcttcc tcctcacagc tgctcgggga
    18541 cgagcgcgcc gctcgcgtag gagctcgctc gctggcgctc gtactcctcc catgcctgct
    18601 tcccccagat ctccaggcgg tcgcgaacgc ccacgatcac gacctccttg cccagccgcg
    18661 ccgtctcccg gagctggcgc gggaccacca ggcggccctg ggagtccagg tccacctgct
    18721 cggccttccc gaagaagatc cgcgcgtagt cgcgggcctt cgggtcggag atg
//
LOCUS       DASUTZ010000098        22350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_933391, whole genome shotgun sequence.
ACCESSION   DASUTZ010000098 DASUTZ010000000
VERSION     DASUTZ010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 22350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22350
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933391"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VF097_04350"
     CDS             <1..862
                     /locus_tag="VF097_04350"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6262059.1"
                     /translation="EAAFGGTLSQAPLRSHVKVGEADLLLMPAVERGGGAGVKLVTVN
                     PENPDRGLPLIHGVYVLFAPGSLEPVAVVDGAALTGLRTAAVSGVATRHLALPDASRL
                     VIFGAGTQARTHLDAMIAVRPIERLTVVSRTAERAQDLVERARDLGLDARVGEPSNVR
                     EADIVCTCTTSPTPVFSGGDLSDGVHVNAVGAYTPDTRELDAETLRRGRVVVETREAA
                     LAEAGDLLLAIEEGAFSTDAIVADLTQVVRGTEVRTSDGDLTVFKSVGVAFEDLAVAR
                     AALDRLEESV"
     gene            859..2007
                     /locus_tag="VF097_04355"
     CDS             859..2007
                     /locus_tag="VF097_04355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6262060.1"
                     /translation="MRAADVVIVGGGCVGASAAYHLTAAGAGSVLLLERADDLGTGST
                     AAAAGGFRHQFGSEVNVRLSLASIPMLIRFEKEHGIPLDVHQDGYLLLVRDESSWAGF
                     RRAAEMQRGLGVRVELLTGAEAAELIPGLAVDEVVGATFGPDDGIADTSGLTHGYAAL
                     ARRAGAQIETGVDVAELLAEGDRVTGVRTAVGEEISAGVVVNAAGPWAGRLAATAGVD
                     LPLDPIPRNVVVTGPFPGAPGRRTLVVDTPTSFYFHREGRGVLMGMGSRAEQPSFDIS
                     VDHSFVADEILPTAMRVFPPIEEAGVEHTWCGLYEMTPDRHPILGEAVPGLYLANGFS
                     GHGFQHSPIVGKLLAEMIVEGAASTVDVSSLSLDRFERGRPVVETHVI"
     gene            2007..3038
                     /locus_tag="VF097_04360"
     CDS             2007..3038
                     /locus_tag="VF097_04360"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6262061.1"
                     /translation="MDADVIVVGAGAVGSAAARDLARRGLDVIALERSRIGHDRGSSH
                     GDARVFRHSYDAPGWVRMMKEAMGLWAELEDEAGEVLLTRTGGIDLDPPPQNAASLDA
                     EGVGYEVLPGAAVTERFPSLSLRPEAKVLFQADAGIVHADRAWRALAQGARRAGADVR
                     EGVRVLELRTDEDGVEARTEGGSISARVGVVAAGSWTATLLRPLGISLPVRVTRESPA
                     YFESDPPPVVVVDWHGEHLTYALPSPRFGLKAAEHHAGPEVDPDDGGGPDQASVDRVR
                     AWVAERFAPMDPRPLHAEACLYTTTADERFLLERHGAVVVASACSGHGFKFVPWSGAR
                     AAELASRRS"
     gene            3035..4465
                     /locus_tag="VF097_04365"
     CDS             3035..4465
                     /locus_tag="VF097_04365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX6262062.1"
                     /translation="MTARTKVMAAVAAVAVAIGGIVIIVREGPSEEPEVARGPLPGPM
                     ASPSPSLEPPGPALRVRVGRVSAVTVGGRPRRPRLRAARRGVERTIGELYTRAFLEPV
                     GGGGAPRIDDLFVPQTRRAARRDSGILTLGDAAARITAVRAPRARLDLRVLTDRGRRP
                     TDVVARVRFAARAFRGEIEDEIRHRGSYLLRRAAGRWRIVSYDVRGRIPPARKVARPM
                     GAPATRRGVLFILAIGSDARPGERVAGARADSLHIIGVNFRKGSVGIVGIPRDSFVPI
                     PGVGTRKINEALFYGGPELTVRTVERLSGIRIDAYLLTGFQGFRSAVTGVGGVRTVIP
                     YAMSDAASEAFFRPGRRRLGGRGALAFSRNRHDAPGGDFGRSLNQGRLLMAALREFRS
                     DLRKDPAALLRWVAVGARNLQTDLSLQDTLGLLLAATSVDLSKVRNTVVSGSGAMVGG
                     QSIVRLGSGAQATFRDLRTDGLLNGR"
     gene            complement(4462..4881)
                     /locus_tag="VF097_04370"
     CDS             complement(4462..4881)
                     /locus_tag="VF097_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HEX6262063.1"
                     /translation="MAEGRALAAGMAEGVLIVLREPLSFWGGMDPETGEVIDAHHPQR
                     GEILTGRVVAMPSGRGSSSASSVLAEAIRIGKAPVALVLMEPDPIVALGSVVASELYG
                     SACPVVVLDEAAYERLRTGDSVRITADERGARVEALA"
     gene            complement(4874..6139)
                     /locus_tag="VF097_04375"
     CDS             complement(4874..6139)
                     /locus_tag="VF097_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017330295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HEX6262064.1"
                     /translation="MDVRLDDRDRELLDGAHGEAGSLAMRILVRMARAQGAARLRDVT
                     RAHIDGCLYHGRAGLDFARRMVEGGGRVAIPTTLNVASLDLLHPERYRGDPETARQAR
                     ALMDAYVELGCRPTWTCAPYQLPDARPAVGEHVAWAESNAIVFANSVLGARTDRYGDF
                     LDLCAAITGRVPDAGLHLDERRRGEVVFRVEGIGSGLLADPVLYPLVGHVVGTATGSR
                     VPILVGLPGDASEDDLKALGAAAASSGSVALFHAVGLTPEAPTLDEALGGRPPLREEA
                     IGRERLRAARDELTTARGDRLSGVSLGTPHFSVGEFRTLLDALDGERIHPDVECYVST
                     GRDVLAEVESRGWLAPVEAAGVRIVTDTCTYVTPIMRPGGVVMTNSGKWAYYAPGNLR
                     TATVFGSLEECVRSAVAGRVTVDEGLWRG"
     gene            complement(6141..7121)
                     /locus_tag="VF097_04380"
     CDS             complement(6141..7121)
                     /locus_tag="VF097_04380"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="cyclodeaminase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6262065.1"
                     /translation="MLLLSEEEIRRSVSSKEALEAVAEAFAALARGEARLPGVIYLDL
                     QEFDGDVHVKGAHLRGASHFAIKVAGGWYDNPSRGLPVGTGLVLVFSAETGAPAALLL
                     DNGYLTDIRTGAAGALAARHLARDPLAKVAMIGAGLEARFQLRALADVRALPRIEAWS
                     RDPANARRFAEEMRAELGADVEVAPDVESAVREADLVVTATPAREALVRADWLSPGAH
                     VTALGSDGPAKQELDVDVLARADLVVVDDLEQAAEKGELHHALEAGALTRDDVAAQLG
                     DLVTGDHPGRTGGGQITVADLTGVGVQDAAVAALALERAEAAGLGRDLPT"
     gene            complement(7121..8281)
                     /locus_tag="VF097_04385"
     CDS             complement(7121..8281)
                     /locus_tag="VF097_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX6262066.1"
                     /translation="MSGSLVPKDDFPVPDATTYLNSASISLTARPVREEMRRFEDEVV
                     HLGTINLDDEAENRVYEGVRGSAAELLGCAADDIAVMTSATEALCQVAWWLRPGRGEN
                     VVSIDIEFPSVTYPWLRLAEDEGLGVRLVRALEDPLRLTLDGVAELVDDRTAAICVSH
                     VQYATGHLLDPAALADLAHAHDALLILDTTQSSGAVPLDVRSTDQDVVIASAYKWLTG
                     VPGAAFAYLRPEVAERFRPPFVGWRTTGEGAMAFDATDISLALGGRRMEYATVAYEAG
                     ISLGAATRYLLDVGIEKIHEHDRALCADLAEGLRARGAEPLTPEDPDARGTIVAARFP
                     GRDPFELAGALARRGVFVAPRLGGLRFSPHLHNDEDDVARALEALDAALGDA"
     gene            complement(8301..9467)
                     /locus_tag="VF097_04390"
     CDS             complement(8301..9467)
                     /locus_tag="VF097_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX6262067.1"
                     /translation="MWSSLAPRADFPVLEEAAYLNAASIALMPLPVRRAMQAFMEEIA
                     GRGTIGFDDDAEAAVYDGVRSRAARLLGAEPADVAVTTSASEALGQVAWWLRPGEGEN
                     VVSIDIEFPSVTYAWMRVAEETGVEVRLVRALEASGGLSLEDVAELVDDRTAAVCVSH
                     VQYATGHLLDLRGLADLAHAHGALAVVDATQSAGMIPIDVREADVDVLVSGGYKWLCA
                     TFGAALCYLHSRIAERFLPPLIGWRSTPNPPDFDATRMPLAEGARRLEYSTVAYPSGI
                     GLGEAIEYILGLGVDRILEHDLGLTDKVTAGLEELGGTLISAPRDVPRGSIATARFPG
                     RRPERLVERLIEADVIAGPRLGGVRISPHLYNDVSDVDRALAQLERILETPEDD"
     gene            9580..10428
                     /locus_tag="VF097_04395"
     CDS             9580..10428
                     /locus_tag="VF097_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6262068.1"
                     /translation="MRKQRYLWLIAALAALMLVASACGGDEDTDAEPTAEPTETEETA
                     PALETLQEGVLTVGSDIPYPPFEYREGGELTGLDIDLITEMATRLGLEVEVIDTDFDT
                     IFTQLAGGRFDVVASATTITPEREEEVNFTEGYYNAQQSLTVLADSGITSVDELGEEH
                     SVAVQRGTTGEAWAEENLAPQGVTLRTFTQTPDLYSAVEGGAVTGAINDEPSAIAEVA
                     AREGLELVETIDTGEVYGIAVNPANEPLLEELNRVLAEMIEDGTYEEIYSAYPDLPPG
                     GNVAAG"
     gene            10509..11609
                     /locus_tag="VF097_04400"
     CDS             10509..11609
                     /locus_tag="VF097_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX6262069.1"
                     /translation="MDSRGGRLREWVAHRRRHVAHRAEGQNTLSLAVLIVGIASLALV
                     LVGIPLVITARGWIPDATILESVQSGPATMLLFAAVAGGLVAAAVGFGVYRRMATKPS
                     RKAAISGAALGVQAVVAAGLLLLVRSGENFAFLVRQNLRFEVLEGFLPDFITAIKNTI
                     LLAFFGELGGIVIGLILAMMALSRHAVVRAPARAYINFFRGTPLLWQLSFFYLGFVLG
                     LGLAQTVTVFQAAVFIFSLNAAAYTAEVFRAGIQSIERGQMEAARSLGMTYFQAMRYA
                     ILPQAFRRVIPPLTNEFIILIKDTSLVTLLGLTADQRELLTWARDLYAETFNATPWLA
                     AAAGYMIVTLPMIRAVSWLERKLRSGLVGVGA"
     gene            11624..12346
                     /locus_tag="VF097_04405"
     CDS             11624..12346
                     /locus_tag="VF097_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009439596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX6262070.1"
                     /translation="MVRLEGINKWFGENHVLRDVDLAVNPQDVLVLIGPSGSGKSTLL
                     RTINLLEEPQEGSVIWDGKDITDVRADVDDVRTEIGIVFQSFNLFPHMTALSNVTIAL
                     RKVLRLDDSAAEDRAREQLDHVGLAEKADSYPSQLSGGQQQRVAIARALAMRPKLMLF
                     DEVTSALDPELVKEVLDVMQRLASEGMTMVVVSHEMGFAREVGNRMVFIDEGRILEEG
                     RPKEMFAEPQHERTRSFLSRIL"
     gene            complement(12769..13485)
                     /locus_tag="VF097_04410"
     CDS             complement(12769..13485)
                     /locus_tag="VF097_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019009953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEX6262071.1"
                     /translation="MIGTLINVATVLAGTLAGTLFGDRLPERIRETVLHSLGLITLLV
                     GLDAGLHAFRAPLSETTRASVILVMGAVLVGGILGELLHIERGLNRLGDRLKARFGGG
                     QARFTEGFVVASLVFCVGPLTILGAIQDGATGDFQLLAIKATLDGFAAVAFASALGWG
                     VGFSAITVLVYQGALSLTAAAAASIFTDVVVEAMTAAGGVIIVGIGLRLLEIREVRVG
                     NLLPALVLAPVAFAVVERVG"
     gene            complement(13482..13925)
                     /locus_tag="VF097_04415"
     CDS             complement(13482..13925)
                     /locus_tag="VF097_04415"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX6262072.1"
                     /translation="MSENRTPPGFTREQALATAREIGRGTFLERIELEWLEVGLDRLV
                     ARIPVAGNTQPYGILHGGATAALCETVGSFGTSIHVGLDRRPVGVQLSMNHLRAVREG
                     HVTATGVPLHVGRTIALWEMRVEDDEGRLVAVGTLTLVIRDAVPG"
     gene            complement(13956..14621)
                     /locus_tag="VF097_04420"
     CDS             complement(13956..14621)
                     /locus_tag="VF097_04420"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX6262073.1"
                     /translation="MAAGGSHQEDYLEAIYEMAEEGIPTQQARIAEWLGVTPASVSEA
                     VKRLVQRGLVEVGADRRLVFTTEGRALAETLVRRHRLAERFLVEVVGLPWYLSHEEAT
                     VWGRAISSAIEERFVAMLDDPATCVHGNPIPGTRRPVDQRDLRPLPALPEGSSGRLER
                     LTEDLELDLDVMRFLQEAGLMPGAGVTVASVGPDGTRTLEVDGDRVALGPEIADNLWV
                     RTT"
     gene            14650..17277
                     /gene="polA"
                     /locus_tag="VF097_04425"
     CDS             14650..17277
                     /gene="polA"
                     /locus_tag="VF097_04425"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX6262074.1"
                     /translation="MTERLFLLDGHSLSYRAFFALPPDLATTTGQVTNAVYGFTSMLI
                     KLLAEEHPEYVAVAFDKGRPTVRLEQYAEYKAGRPETPDEFRQQLPLIREVLEALRIP
                     IIEVEGHEADDAIATIAERAVEKGLEVVIVTADRDFFQLVRPGIRVKFNRKGISDIVD
                     YDEKAVTERFGVPPDKYVELVALKGDTSDNIPGVPGVGDKTAAKLITEHGTVEGVLEH
                     AADLTPKLRAGIEEAGDDLLRNRELVQLVTDLDLDIEPQECRVGEWDQDQIRTLFNAL
                     EFRSLLERLQDVGEPRPAAADVELRIEEGDAEAVAAVLERPRPAAVALRATQGAPSAI
                     AVSAGDGRAVHAPLDGSLPGPIRAWLEDPDRPIWVHDAKELQTALLPIGVEAAAIAVD
                     TMLAGYLLDPAEASYPIGALSDRYLGADLVGEASEGPEEDGQLALQEEDRGPLAAEAA
                     AVAILAPLMAERLERQGAADLLRDVELPLATVLARMQAAGVKIDVPYLREMSESLGDR
                     MRTLEAELYEHAGEEFNLNSPPQLRVILYEKLGLTPGKRTKTGYSTDAATLEKLRHQH
                     PIVDALLSYRELAKLKSTYLDALPPLVSPATGRVHTTFSQVAAATGRLSSFNPNLQNI
                     PVRGEQGREIRKAFIAEEGRVLLVADYSQIELRVLAHLSGDEALGAAFAEDADIHAAT
                     AAKVFGLPPEQVDSELRRRAKVVNYGLAYGMNAYGLATRLGIPPDEAKEFIDAYFEGF
                     PRVREFLDHQVTMAAAEGFTQTILGRRRYLPELQSPNPRVRDLGRRMALNAPIQGSAA
                     DIIKLAMIRVDQRVVELPATMVLTVHDELVLEVEEASVEEVAGAVREEMSSAYELSVP
                     LRVDVGSGPTWADAVPEGH"
     gene            17460..18791
                     /gene="rpsA"
                     /locus_tag="VF097_04430"
     CDS             17460..18791
                     /gene="rpsA"
                     /locus_tag="VF097_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX6262075.1"
                     /translation="MTPEELAKAIEESVKDLNDGDLVTGQVVRIDPDEVLLDIGYKSE
                     GVIPTKELSIRHDVDPSEEVSIGDELEALVLQKEDKEGRLILSKKRAQYERAWGRIEE
                     VMESGGTIEGPVIEVVKGGLILDIGLRGFLPASLVDLRRVRDLHPFVGQVLECKIIEL
                     DRNRNNVVLSRRAFLEESQSESRKKVLESLQPGERRTGKVSSIVNFGAFVDLGGVDGL
                     VHVSELSWRHVEHPGEVVSVGEEVEVEVLDVNLERERVSLSLKATQEDPWKEFERKYN
                     AGEVIEGEVTKLVPFGAFVRVADDIEGLVHISELSDAHVESADEVVSVGDRVRVKVVD
                     VDVSRRRISLSMRRVEGDAIITPEFEPEPDEVVPEVEAEAEAEPEETVEEAPQPQAEA
                     EAQAEAAPEEAEAEPEITPEETPAAEDAGQDEEDVSLEDIVADLRRREGRQ"
     gene            18802..19401
                     /gene="coaE"
                     /locus_tag="VF097_04435"
     CDS             18802..19401
                     /gene="coaE"
                     /locus_tag="VF097_04435"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015032826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX6262076.1"
                     /translation="MLLVGLTGGLGAGKSTVARMLAERGAVIVDADELARRSIEPGTR
                     GHRKVLEVFGDEVEARGGSIDRRALADVVFEDPEARRALESITHPEVFRMLAEEVERR
                     RDSGDVVVFDAPLIVETGMDTAMDLLVVVAVAPDTQVDRVVARGMDRAEAEARIAAQA
                     SQEEREAKADVILRNDGDLRDLERQVDALWDRLRREAGG"
     gene            19422..20135
                     /locus_tag="VF097_04440"
     CDS             19422..20135
                     /locus_tag="VF097_04440"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX6262077.1"
                     /translation="METKAVFFDAGETLVHPHPSFPELFAEVLRDHGHEVDPETVQEL
                     AVGASQRFTETLRSDEHALWSTSADRSRRFWFSMYMGLLGDTGIEDRDGRLTDALYAR
                     FSDLESYRLHPDALPLLERLEAGGYAMGVISNFEEWLEGLLERLAVARFFPVRVVSGI
                     EGVEKPDPAIFRIALDRMGVAAEASVYVGDHPYFDVEAAREVGMRPVLIDRRGLYPDA
                     GGVRITSLEDLPEAVGLPV"
     gene            20135..20503
                     /locus_tag="VF097_04445"
     CDS             20135..20503
                     /locus_tag="VF097_04445"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEX6262078.1"
                     /translation="MQERRFTLEEAEAELPHLRELLPQLREARRRVLASGERVRGIAR
                     RNGGGREGTDYWRAIEALRRGVEDLAQRGIVLRDAEGGLVDFPSEREGRTVYLCWRLG
                     EDRIGHWHDVDAGFSGRRPL"
     gene            20528..>22350
                     /gene="uvrB"
                     /locus_tag="VF097_04450"
     CDS             20528..>22350
                     /gene="uvrB"
                     /locus_tag="VF097_04450"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013426002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX6262079.1"
                     /translation="MSSFRVQTDLRPAGDQPKAIDELTEGLAAGDDRVTLLGITGSGK
                     TFTAANVIERLGKPTLVISPNKSLAAQLANEFREFFPDSAVEYFVSYYDYYQPEAYVP
                     QTDLYIEKDATVNEEIDRLRHAATAALLTRKDVVIVASVSCIYGLGSPEEYEQQILHV
                     VRGRPLGHTAALQRLVDMQYQRNQANLARGTFRVQGDTLEIHPSYQETALRIELWGDE
                     VERVLEMDLITGEVVRELDHVTIFPATHYVASRERLDRAIRGIEEELGVRLEQLERTG
                     KLLEAQRLRMRTNHDLEMLREVGFCSGIENYARHIDGRAPGTAPYTLIDYFPDDWLCI
                     IDESHVTVPQLHAMYEGDMSRKRTLVEHGFRLPSAVDNRPLKFDEFVERVNQVMFMSA
                     TPSDYELEVSKRVVEQIVRPTGLVDPEVEIRPTRGQVDDLMEEVRRRAEADQRVLVTT
                     LTKKMAEDLTDYLIEMGLRVRYLHSEIDTIERIQIIRDLRLGEYDVLVGVNLLREGLD
                     LPEVSLVAILDADKEGFLRSETSLIQTIGRAARNVEGKVLMYADEVTDSMQTAISETQ
                     RRRRIQLEHNREHGIDPQTIRKKVSDILLTMVEERPETAPK"
BASE COUNT         3257 a         7745 c         8243 g         3105 t
ORIGIN      
        1 cgaggcagcg ttcggtggga ccctttctca ggccccgctc cgctcgcacg tgaaggtggg
       61 ggaggccgac ctgctgctga tgcccgcggt ggagaggggt gggggagccg gggtgaagct
      121 cgtcacggtg aatccggaga acccggaccg agggctgccg ctgatccacg gcgtgtatgt
      181 cctgttcgcc cccggctcgc tggagcccgt ggcggtcgtg gacggtgcgg cgctcaccgg
      241 cctccggacg gcggcggtct ctggggtcgc cacacgccac ctcgccctgc cggacgcctc
      301 gcgcctcgtg atcttcgggg cgggcacgca ggcccggacc cacctcgacg cgatgatcgc
      361 cgtgcgcccg atcgagcgac tcaccgtggt gtcgcgaacg gcagagcggg cccaggacct
      421 cgtggagaga gcccgagatc tgggcctcga cgctcgcgtg ggggagcctt cgaacgtgcg
      481 tgaggcggac atcgtatgca cctgcacgac gagcccgacg cccgtcttct ccgggggcga
      541 tctgtccgac ggcgttcacg tcaacgccgt gggtgcctac acgcccgaca cgcgggagct
      601 cgacgccgag acccttcggc gggggcgggt cgtggtggag acgcgggagg cggcgctggc
      661 cgaggctggc gacctcctgc tggcgatcga ggagggcgcg ttctcgacgg acgccatcgt
      721 cgccgacctg actcaggtgg tccgggggac cgaggtccgc acctcggacg gcgacctcac
      781 cgtcttcaag tctgtggggg tggccttcga ggacctggcg gtggcccgtg ccgccctgga
      841 ccggctggag gagtccgtgt gagggccgcg gacgtcgtga tcgtgggagg cggatgcgtg
      901 ggcgccagcg ccgcctacca cctgacggca gcgggggcgg gctcggtcct cctgctggag
      961 cgagcggatg acctgggcac gggctccacg gccgcggcag cgggggggtt ccgccatcag
     1021 ttcggcagcg aggtgaacgt acggctgtcc ctggccagca tcccgatgct catccggttc
     1081 gagaaggagc acggcatccc gctggacgtt caccaggacg gctacctgct cctggtgcgc
     1141 gacgagtcgt cgtgggcagg gttccgccgc gcggccgaga tgcagcgagg gctgggtgtc
     1201 cgcgtcgagc tgctaaccgg agccgaggcc gcagagctca tcccgggcct ggcggtggac
     1261 gaagtggtgg gggcgacctt cggccccgac gacgggatcg ccgacacctc cggcctcacg
     1321 cacggctacg ctgcgctcgc gcgcagggcg ggggcgcaga tcgagacggg ggtggacgtc
     1381 gccgagctcc tcgccgaggg ggatcgggtc acgggtgtga gaaccgcagt gggagaggag
     1441 atctcggctg gcgtcgtcgt caacgcggcg ggaccgtggg ccggacggct cgcggccacc
     1501 gccggagtgg atctgccgct ggacccgatc ccccgcaacg tggtggtgac cggcccgttc
     1561 ccgggggccc ccgggcgccg gactctcgtc gtggacaccc ccaccagctt ctacttccac
     1621 cgagagggac gaggtgtgct gatgggcatg gggtcccgag ccgagcagcc ctcgttcgac
     1681 atctcggtcg atcactcgtt cgtcgccgac gagatcctgc cgaccgcgat gcgcgtcttc
     1741 cccccgatcg aagaagcggg ggtggagcac acgtggtgcg gactctacga gatgacgccc
     1801 gatcggcacc ccatcctggg ggaagcggtg ccgggcctct acctcgcgaa cggcttcagc
     1861 ggccacgggt tccagcactc gccgatcgtg gggaagctgc tggccgagat gatcgtggag
     1921 ggcgcggcat ccacggtgga cgtctcctct ctctctctgg accggttcga gcgaggtcgg
     1981 cccgtggtgg agacccacgt catctgatgg acgccgacgt catcgtcgtc ggggcggggg
     2041 ccgtggggtc ggcggccgcg cgcgacctgg cccgccgcgg tctcgacgtg atcgccctgg
     2101 agcgctcccg gatcggccac gaccggggct cgagccacgg cgacgcgcgg gtcttccggc
     2161 attcctacga cgctcccggg tgggtccgca tgatgaagga ggcgatgggc ctctgggccg
     2221 agcttgagga cgaggcgggg gaggtgctgc tgacgcggac gggcgggatc gatctcgacc
     2281 ccccgcccca gaacgctgcg tcgctcgacg ccgagggcgt gggttacgag gtgctgccgg
     2341 gcgccgccgt gacggagcgg ttcccctccc tgagcctccg gccggaggcg aaagtgctgt
     2401 tccaggccga cgccgggatc gtccacgcgg atcgggcgtg gcgggcgctg gcgcagggcg
     2461 cccgccgggc gggcgcggac gtccgggagg gcgtccgggt cctggagctc aggacggacg
     2521 aagacggggt ggaggcccgc accgaagggg gctcgatctc ggcgcgcgtc ggcgtcgtgg
     2581 ccgccgggtc gtggaccgcg accctccttc gtcccctcgg catctccctc ccggtgcgcg
     2641 tcacccggga gtcaccggcg tacttcgagt ccgatccgcc gccggtcgtc gtcgtcgact
     2701 ggcacgggga gcatctaacc tacgcgctgc ccagcccgcg gttcggtctg aaggcggcgg
     2761 agcatcacgc cgggcccgag gtcgatcccg acgacggggg tggtcccgac caggcctccg
     2821 tggacagggt gcgggcctgg gttgccgagc gcttcgcccc gatggacccg aggccgctgc
     2881 acgcggaggc gtgcctctac accacgactg ccgacgagcg cttcctgttg gagcggcacg
     2941 gggccgtggt cgtcgcctcc gcgtgcagcg gccacggctt caagttcgtc ccgtggtcgg
     3001 gcgcgcgggc cgccgagctc gcctcgagga ggtcatgacc gcccggacga aggtgatggc
     3061 cgccgtggcc gccgtggcgg tggcgatcgg cgggatcgtg atcatcgtgc gggaaggccc
     3121 cagcgaggag cccgaggtgg cccgtggccc cctaccgggc ccgatggcgt cgccgtctcc
     3181 ctcgctcgag ccgcccggcc ccgcgctccg cgtgcgggtg ggcagggtgt cggccgtgac
     3241 ggtcggcggg cgtccccggc gcccgcggct tcgtgccgcc cgacgaggcg tcgagcggac
     3301 gatcggcgag ctgtacacca gggcgttcct tgagccggtg gggggcggtg gggctccccg
     3361 catcgacgat ctcttcgtcc cgcaaacccg gagggcggcc cgacgggaca gcgggatcct
     3421 gaccctcggc gatgccgcgg cccggatcac ggccgtccgg gcgccacgcg ctcgcctcga
     3481 cctgcgggtc ctcactgatc ggggccgccg gcccaccgac gtcgtggctc gcgtgcgctt
     3541 cgcggcgagg gccttccggg gcgagatcga ggacgagatc cggcaccgcg gatcgtacct
     3601 gctccgacga gcggcgggtc gctggcgcat cgtgtcctac gacgtccggg ggcggatccc
     3661 gccggccagg aaggtagcgc ggccgatggg ggcgccggcg acgcgccggg gggtgctctt
     3721 catcctggcg atcggcagcg acgcgcgacc cggggagcgc gtcgccggcg ctcgcgcaga
     3781 ctcccttcac atcatcggcg tcaacttcag gaagggaagc gtgggcatcg tggggatccc
     3841 tcgtgattcc ttcgttccga tccccggcgt gggcacccgc aagatcaacg aggcgctctt
     3901 ctacggcggc ccggagctca cggtgcggac ggtggagcgg ctgagcggca tccggatcga
     3961 cgcctacctc ctcacgggct tccaggggtt ccgcagcgcg gtgaccggcg tgggcggtgt
     4021 gcggacggtg atcccctacg cgatgagcga cgccgcgtcc gaggcgttct tccgccccgg
     4081 ccggaggcgt ctcggaggtc ggggtgcgtt ggcgttctcc cgcaaccgac atgacgcgcc
     4141 gggcggcgac ttcgggcggt cgctgaacca ggggcggctg ctgatggcag cgctgcgcga
     4201 attccggtcc gacctgcgga aggatccggc cgcgctgctg cggtgggtgg ccgtcggcgc
     4261 tcgcaacctc cagacggacc tcagccttca ggacacgttg ggactgctcc tggccgccac
     4321 gagcgtggac ctttcgaagg tccggaacac cgtggtctcg gggagcgggg ccatggtcgg
     4381 agggcagagc atcgtgcgcc tgggctcggg ggcgcaggcc accttccggg acctcaggac
     4441 cgacggcctc ttgaacggtc gctaggcaag cgcttcgacg cgggcgccgc gctcgtcggc
     4501 ggtgatcctg accgaatcgc cggtccgcag ccgctcgtag gccgcctcgt ccagcaccac
     4561 gacgggacag gcggacccgt agagctccga ggccaccacg cttcccaggg ccacgatcgg
     4621 gtccggctcc atgagcacca gggcgacggg ggccttcccg atccgtatgg cctcggccag
     4681 gacgctggag gcgctgctgg agccgcgccc ggacggcatg gccacgacgc gtccggtgag
     4741 gatctcgccg cgctgggggt ggtgcgcgtc gatcacctcg ccggtctccg ggtccatccc
     4801 tccccagaag gagagcggct cccggagcac gatcaggacc ccctcggcca tccccgcggc
     4861 caaagcgcgt ccctcagcca cgccacagcc cctcgtccac ggtgacccgt ccggccaccg
     4921 ccgaccggac gcactcctcg aggctgccga agacggtggc agtccggagg ttacccgggg
     4981 cgtagtaggc ccacttcccg gagttggtca tcacgacgcc tccgggccgc atgatcgggg
     5041 tcacgtaggt gcacgtgtcg gtcacgatgc ggacgccggc ggcctcgacc ggcgccagcc
     5101 agcccctgga ctccacctcg gccagaacat ccctgcccgt cgagacgtag cactcgacgt
     5161 ccggatggat ccgctccccg tccagcgcgt cgaggagcgt ccggaactcc ccaaccgaga
     5221 agtggggagt gccgaggctg accccggaga gccgatcgcc gcgggcggtc gtgagctcgt
     5281 cccgcgcggc ccggagccgc tcccgcccga tcgcctcctc gcggagcggg ggccggccgc
     5341 cgagtgcctc gtcgagcgtg ggcgcctcgg gcgtcagccc cacggcgtgg aagagcgcca
     5401 ccgagcccga ggacgcggcg gcggcgccga gcgccttcaa atcgtcctcg gaggcgtccc
     5461 cgggcaggcc cacgaggatg ggcacccggg agcccgtcgc cgtccccacg acgtggccga
     5521 cgagggggta gaggaccggg tcggccagca gccccgagcc gatcccctcg acccggaaca
     5581 cgacctcgcc tcgacggcgc tcgtcgaggt ggagccccgc atcggggacc cgcccggtga
     5641 tcgccgcgca gaggtcgagg aagtcgccgt accggtcggt ccgggccccc aggaccgagt
     5701 tggcgaagac gatcgcgttg gactcggccc aggccacgtg ctcaccgacg gctggtctcg
     5761 cgtcggggag ctgatagggc gcgcaggtcc acgtgggccg gcacccgagc tccacgtacg
     5821 cgtccatcag ggcacgggcc tggcgggcgg tctcgggatc gccccggtag cgctcgggat
     5881 ggaggaggtc gagcgaggcc acgttgaggg tggtcgggat cgccacgcgg ccgcctccct
     5941 cgaccatgcg tcgggcgaag tccaggccgg cccggccgtg gtagaggcag ccgtcgatgt
     6001 gggccctggt gacgtcgcgg agccgtgccg cgccctgcgc ccgggccatc cggacgagga
     6061 tgcgcatggc gaggctgccg gcctcgccgt gggccccgtc gaggagctcc cggtcccggt
     6121 cgtcgagacg gacgtccacc tcaggtgggg aggtcgcggc cgaggcccgc cgcctcggct
     6181 cgctccagcg ccagcgccgc caccgcggcg tcctggacgc ccacgccggt caggtccgcc
     6241 acggtgatct ggccgccgcc cgttcggccc ggatggtcgc cggtgacgag gtccccgagc
     6301 tgcgccgcca cgtcgtcccg cgtgagggcg ccggcctcca gcgcgtggtg gagctcgccc
     6361 ttctcggcgg cctgctcgag gtcgtccacg accacgaggt cggcgcgggc caggacgtcc
     6421 acgtccagct cctgcttcgc gggtccgtcc gagccgaggg cggtgacgtg ggccccgggg
     6481 gagagccagt cggcccgcac gagggcctcg cgggccggcg tggcggtcac gacgagatcg
     6541 gcctctcgca ccgccgactc gacgtccggg gccacttcga cgtccgcccc cagctcggcc
     6601 cgcatctcct cggcgaaccg ccgggcgttg gcggggtcgc ggctccaggc ctcgatcctg
     6661 gggagcgccc ggacgtcggc cagggcccgc agctggaacc gagcctcgag cccggcgccg
     6721 atcatcgcga ccttggcgag cgggtcgcgg gccaggtggc gggcggcgag ggcgccggcg
     6781 gcgcccgtcc gaatgtcggt gaggtacccg ttgtcgagca ggagcgccgc cggcgccccc
     6841 gtctccgcgc tgaacacgag gaccagcccg gtccctaccg gcaggcccct cgacgggttg
     6901 tcgtaccagc ctccggccac cttgatggcg aagtgcgagg cgccccgcag gtgagccccc
     6961 ttcacgtgca cgtccccgtc gaactcctgg aggtccaggt agatgacgcc cgggaggcgg
     7021 gcctcgccgc gggccagggc cgcgaaggcc tcggccacag cctccagggc ctccttcgac
     7081 gagaccgaac gtcggatctc ctcctcggac agcaggagca tcaggcgtcc cccagggccg
     7141 cgtcgagcgc ctcgagggcc cgggccacgt cgtcctcgtc gttgtggagg tgcggcgaga
     7201 accggaggcc cccgaggcga ggggcgacga agaccccccg ccgggctagg gcgccggcca
     7261 gctcgaacgg gtcccgcccc gggaagcgcg ccgccacgat cgtcccccgg gcgtcggggt
     7321 cctccggcgt cagcggctcc gcgccgcgcg cccgcagccc ctcggccagg tcggcgcaga
     7381 gcgctcggtc gtgctcgtgg atcttctcga tgcccacgtc caggaggtag cgggtcgccg
     7441 cgcccagcga gatccccgcc tcgtaggcca ccgtcgcgta ctccatccgc cgcccaccca
     7501 gtgccagcga gatgtccgtg gcgtcgaagg ccatcgctcc ctcgccggtc gtccgccagc
     7561 ccacgaacgg cgggcggaac cgctcggcga cctcgggtcg caggtacgcg aacgcggcgc
     7621 ccggcacccc cgtgagccac ttgtaggcgc tggcgatcac cacgtcctgg tcggtggacc
     7681 gcacgtcgag cgggacggca cccgacgact gcgtggtgtc gaggatcagg agggcatcgt
     7741 gcgcgtgggc caggtcggcc agcgccgccg ggtcgaggag atgcccggtg gcgtactgca
     7801 cgtggctcac gcagatggcc gccgtccggt cgtccacgag ctccgccacc ccgtcgagcg
     7861 tgagccggag cggatcctcc agcgcccgca ccagccggac accgagcccc tcgtcctccg
     7921 ccagccgcag ccaggggtag gtcacgctgg ggaactcgat gtcgatcgag acgacgttct
     7981 cgccccgccc cggtcggagc caccaggcca cctggcagag ggcctccgtg gcgctcgtca
     8041 tgacggcgat gtcgtcggcg gcgcatccga ggagctctgc ggctgaaccc cggaccccct
     8101 cgtacacgcg gttctcggcc tcgtcgtcga ggttgatcgt gccgaggtgc acgacctcgt
     8161 cctcgaagcg gcgcatctcc tcgcggacgg gccgagccgt cagagagatc gacgccgagt
     8221 tgagataggt cgtcgcgtcg gggaccggga agtcgtcctt gggcacgaga gagccgctca
     8281 tggccttcct tccgctccgg tcagtcgtcc tccggcgtct ccaggatccg ctcgagctgg
     8341 gcgagggccc ggtcgacgtc ggagacgtcg ttgtagaggt gcggggagat gcggacgccc
     8401 ccgagccggg gcccggcgat cacgtccgcc tcgatcagcc gctccaccag ccgctcgggg
     8461 cgcctccccg ggaaccgggc cgtggcgatg ctgccgcggg gcacgtcccg gggcgcggag
     8521 atcagcgtcc ccccgagctc ctcgagcccc gcggtcacct tgtcggtgag cccgaggtcg
     8581 tgctcgagga tccggtccac gcccagcccc aggatgtact cgatcgcctc gcccaggccg
     8641 atgcccgagg gataggccac cgtggagtac tcgagccgcc gtgcgccctc cgccaggggc
     8701 atccgcgtgg cgtcgaagtc cggggggttg ggggtgctcc tccagccgat caggggaggg
     8761 aggaaccgct ccgcgatccg cgagtgcagg tagcagagcg ccgccccgaa cgtggcgcag
     8821 agccacttgt accccccgct gacgagcacg tccacgtcgg cctcccgcac gtcgatcggg
     8881 atcatcccgg cggactgggt ggcgtcaacg accgccagcg cgccgtgggc gtgggccagg
     8941 tcggccagcc cccggagatc gaggaggtgg ccggtggcgt actgcacgtg gctcacgcag
     9001 acggccgccg tgcgatcgtc gacgagctcg gccacgtcct ccagggagag gcccccggac
     9061 gcctcgagcg cgcgcaccag gcgcacctcg acaccggtct cctccgccac ccgcatccag
     9121 gcgtacgtga cgctggggaa ctcgatgtcg atcgagacga cgttctcgcc ctcgccgggg
     9181 cggagccacc acgccacctg gcccagcgcc tccgaggcgc tcgtggtgac ggcgacgtcg
     9241 gcaggctcgg cccccagcag gcgcgcggcc ctgctgcgga cgccgtcgta gacggccgcc
     9301 tcggcgtcgt cgtcgaagcc gatcgtcccg cgtccggcga tctcctccat gaaagcctgc
     9361 atcgcccggc ggacgggcag gggcatcagg gcgatcgagg cggcgttgag gtaggccgct
     9421 tcctcgagga cggggaagtc ggcgcgcggc gccagcgagg accacatgcg ctcatcctgg
     9481 cacacggccg cgcgctcccg ccacgcgcgc ttgtgatgag cgggcggcct ccctaggatt
     9541 ccgcaggaga acgagggaat ccatgaaggg aggggatccg tgcgcaagca acggtacctg
     9601 tggctgatcg cggcgctcgc cgcgctcatg ctcgtcgcca gcgcgtgtgg gggcgacgag
     9661 gacaccgacg ccgagcccac ggccgagccg accgagaccg aggagaccgc cccggcgctc
     9721 gagacgctcc aggagggggt cctgaccgtc ggttccgaca tcccgtaccc gccgttcgag
     9781 taccgtgagg gcggcgagct gacggggctc gacatcgacc tgatcacgga gatggcgacc
     9841 aggctcgggc tcgaggtcga ggtcatcgac actgacttcg acacgatctt cacgcagctg
     9901 gccgggggcc ggttcgacgt cgtggcctcg gccaccacga tcacccccga gcgtgaggag
     9961 gaggtcaact tcaccgaggg ctactacaac gcccagcagt cgctgacggt gcttgccgac
    10021 agcggcatca ccagcgtgga cgagctgggg gaggagcact cggtggcggt ccagcgcggg
    10081 acgaccggcg aagcgtgggc cgaggagaac ctggccccgc aaggggtgac gctgcggacc
    10141 ttcacgcaga cccccgatct gtactcggcg gtcgagggtg gagccgtgac cggcgcgatc
    10201 aacgacgagc cctcggcgat cgccgaggtc gccgcccgcg agggcctcga gctcgtggaa
    10261 acgatcgaca cgggcgaggt ctacgggatc gcggtgaacc cggccaacga gccgctcctg
    10321 gaggagctga accgggtgct ggccgagatg atcgaggacg gcacctacga ggagatctac
    10381 tccgcgtacc cggacctgcc tcccggcggg aacgtggcgg ccggctgagg gatcgaggag
    10441 cgtcggaggc ggtgggggcg ccgttcgggc gcccccaccg cctccgctga aggacgaggg
    10501 agggcgcgat ggactcgcgt ggagggcggc tgcgcgaatg ggtcgctcat cggcgacgtc
    10561 acgtagcaca tcgagctgag gggcagaaca cgctctcgct ggccgtgctg atcgtgggga
    10621 tcgcgtccct cgcgctggtg ctcgtcggca tcccgctcgt catcacggcc cgggggtgga
    10681 tccccgacgc gacgatcctg gagagcgtcc agagcggccc cgcgaccatg ctgctcttcg
    10741 cggccgtggc cggggggctc gtggcggcgg cggtgggctt cggcgtctac cggcggatgg
    10801 cgacgaagcc ctccaggaag gcggcgatct cgggggccgc gctgggcgtc caggcggtgg
    10861 tggccgccgg cctcttgctc ctggtccgct cgggggagaa cttcgccttc ctcgtccgcc
    10921 agaacctgcg cttcgaggtg ctcgaggggt tcctgcccga cttcatcacg gcgatcaaga
    10981 acacgatcct gctggcgttc ttcggagagc tggggggtat cgtgatcggc ttgatcctgg
    11041 cgatgatggc gctctcacgg cacgccgtgg tccgggcccc ggcccgcgcc tacatcaact
    11101 tcttccgggg gacgcccctc ctgtggcagc tctcgttctt ctaccttggc ttcgtcctgg
    11161 gcctcgggct ggcccagacg gtcaccgtct tccaggccgc cgtgttcatc ttctccctca
    11221 acgcggccgc gtacacggct gaggtgttcc gcgcggggat ccagtcgatc gagcgggggc
    11281 agatggaggc ggcccgatcg ctgggcatga cgtacttcca ggcgatgcgc tacgcgatcc
    11341 tgccccaggc gttccgacgg gtgatcccgc cgctgacgaa cgagttcatc atcctgatca
    11401 aggacacgtc gctggtgacc ctcctggggc tcacggccga ccagcgtgag ctcctgacgt
    11461 gggcccgcga cctctacgcg gagaccttca acgccacgcc gtggcttgcg gcggcggccg
    11521 gctacatgat cgtgacgttg cccatgatcc gcgcggtgag ctggctggag cggaagctcc
    11581 gcagcgggct ggtgggggtg ggggcgtgaa ccccgacggc acgctggtgc gcctcgaggg
    11641 catcaataag tggttcggcg agaaccacgt tttgcgcgac gtcgacctcg cggtgaaccc
    11701 tcaggacgtg ctcgtgctga tcggcccctc gggctcgggg aagtccaccc ttctgcgcac
    11761 gatcaacctg ctggaggagc cgcaggaggg gagcgtgatc tgggacggga aggacatcac
    11821 cgatgtccgt gccgacgtcg atgacgttcg caccgagatc ggaatcgtgt tccagtcgtt
    11881 caacctcttc ccgcacatga cggcgctgag caacgtgacg atcgccctgc ggaaggtcct
    11941 gaggctggac gactcggcgg ccgaggatcg cgcgcgggag cagctcgacc acgtggggtt
    12001 ggcggagaag gccgactcct acccatcaca gctctccggg ggtcagcagc agcgggtggc
    12061 gatcgcgcgg gcgctggcga tgcgtcccaa gctgatgctg ttcgacgagg tgacgtcggc
    12121 gctcgacccc gagctcgtca aggaggtcct cgacgtgatg cagcggctgg cgtccgaggg
    12181 catgacgatg gtggtcgtgt cgcacgagat ggggttcgcc cgcgaggtgg gcaatcggat
    12241 ggtcttcatc gacgagggcc ggatcctgga ggaggggcgg cccaaggaga tgttcgccga
    12301 gccccagcac gagcggaccc ggtcgttcct ctcacggatc ctctagcccc cgtacgctcg
    12361 gcgggctcga ggtgcctttg gagccgctca cgccttcccc atacgcgagc gttgtctact
    12421 cggcacctcg agcccgccaa aacgatggcc ccgagcaacc ttcggcactc cccggccggg
    12481 gccgggcgtc tcggcgggcg tccgggagac cgatctgggc tcgccgtccg ggccggggcc
    12541 cgtccggtgg cctgctccgc tcctccggag cctccgagac ggtcgtccct ccggccgctc
    12601 gggaccgagg cgaaacctaa cagggcggcg aaggcggagt caaggagagg agatatgctg
    12661 tcccgcaagg gttctcgggc aacggcccag gtcgcgggcg ggtcgcccgg cgcaagaaac
    12721 tgggccgccg agcggatccg agtgacaaaa ccccaggaac tgcggcgttc agccaacgcg
    12781 ctccacgacg gcgaacgcga caggggccag aacgagcgcc gggaggaggt tccccacccg
    12841 cacctcgcgg atctcgagca ggcggaggcc gatgccgacg atgatgacgc ctcctgccgc
    12901 ggtcatcgcc tccacgacca cgtcggtgaa gatcgaggcc gcggccgccg ccgtgagcga
    12961 cagcgcgccc tggtagacga ggacggtgat ggccgagaat ccgacgcccc accccagcgc
    13021 cgaggcgaac gcgacggccg cgaagccgtc gagggtggcc ttgatcgcca ggagctggaa
    13081 gtcgccggtg gccccgtcct ggatggcccc caggatcgtt aggggcccca cgcagaacac
    13141 gagcgaggcc acgacgaagc cctcggtgaa cctggcctgg cctccgccaa acctggcctt
    13201 gaggcggtcg ccgagccggt tgaggccccg ctcgatgtga agcagctctc ccaggatccc
    13261 cccgaccagg acggccccca tgacgaggat cacgctggcg cgcgtcgtct ccgacagggg
    13321 ggcccggaag gcatgcagcc cggcgtcgag gccgacgagc agggtgatca ggccgagcga
    13381 gtggaggacg gtctcgcgga tccgctccgg gaggcggtcg ccgaagagcg tgccggccag
    13441 ggtcccggcc aggaccgtgg ccacgttgat gagcgtgccg atcatccggg aacggcgtcc
    13501 cggatcacca gcgtcagtgt gcccacggcg acgagccgcc cctcgtcgtc ctcgacccgc
    13561 atctcccaca gcgcgatcgt gcggccgacg tggaggggaa ccccggtcgc ggtgacgtgc
    13621 ccttcgcgga ccgcgcggag gtggttcatg gacagctgga cccccacggg gcgccgatcg
    13681 agcccgacgt ggatcgaggt cccgaaggat ccgaccgtct cgcagagcgc agccgtggcc
    13741 cctccatgga ggatgccgta gggctgggtg ttaccggcca cggggatccg ggccacgaga
    13801 cggtcgagcc cgacctcgag ccactcgagc tcgatgcgct ccaggaacgt gccccgcccg
    13861 atctccctgg ccgtggccag agcttgctcc cgcgtgaagc cggggggcgt gcggttctcg
    13921 ctcatcggcg ggagtctagc cgagccggct cgggctcaag tggtccggac ccacaggttg
    13981 tcggcgatct ccggcccgag ggccacgcgg tctccgtcca cctcgagggt ccgcgtgccg
    14041 tcgggtccca ccgaggccac ggtgacgccc gccccgggca tcaggccggc ctcctgcagg
    14101 aaccgcatca cgtccaggtc gagctccagg tcctcggtaa gccgctccag cctccccgag
    14161 gacccctccg gcagcgccgg gagcgggcgg aggtcccgct ggtccaccgg tcgcctcgtc
    14221 ccggggatcg ggttgccgtg gacgcacgtg gcggggtcgt cgagcatcgc cacgaaccgc
    14281 tcctcgatgg cggacgagat cgcccggccc cagacggtcg cctcctcgtg cgacaggtac
    14341 caggggagcc ccacgacctc gaccaggaat cgctcggcga gccggtgacg acgcaccagg
    14401 gtctcggcca gggcccgccc ctccgtcgtg aagacgagcc tccggtcggc gccgacctcc
    14461 accagccccc gctggacgag gcgcttcacc gcctccgaga ctgaggccgg cgtgacgccc
    14521 agccactccg cgatccgggc ctgctgggtg gggatcccct cctcggccat ctcgtagatc
    14581 gcctcgaggt agtcctcctg atgggagccc ccggccgcca tgagggagag tctacaatcg
    14641 gcacgcgtgg tgaccgagcg actcttcctg ctggacgggc acagcctctc ctaccgcgcc
    14701 ttcttcgcgc tcccgcccga cctggctacc accaccggcc aggtgacgaa cgccgtctac
    14761 ggcttcacct cgatgctgat caagctcctg gcggaggagc accccgagta cgtggcggtg
    14821 gcgttcgaca aggggaggcc cacggtgcgc ctcgagcagt acgccgagta caaggcgggc
    14881 cgccccgaga cccccgacga gttccgccag cagctcccgc tgatccgcga ggtgctcgag
    14941 gcgctgcgga tcccgatcat cgaggtcgag gggcacgagg ccgacgacgc gatcgccacg
    15001 atcgccgagc gggccgtgga gaaggggctc gaggtcgtga tcgtcacggc cgaccgcgac
    15061 ttcttccagc tcgtgcggcc cgggatccgc gtgaagttca accgcaaggg gatctcggac
    15121 atcgtcgact acgacgagaa ggccgtgacc gagcggttcg gcgtgccccc ggacaagtac
    15181 gtggagctcg tggctctgaa gggcgatacg tccgacaaca tccccggcgt gccgggggtg
    15241 ggcgacaaga cggccgcgaa gctgatcacc gagcacggga cggtcgaggg ggtcctggag
    15301 cacgccgcgg acctcacccc gaagcttcgg gcggggatcg aggaggcggg cgacgatctc
    15361 ctccggaacc gggagctggt tcagctggtc accgatctgg acctcgacat cgagccccag
    15421 gagtgccggg tgggtgagtg ggaccaggac cagatccgca ccctcttcaa cgcccttgag
    15481 ttccggtcgc ttctggagcg cctccaggac gtgggcgagc cgcggccggc cgcggccgac
    15541 gtcgagctca ggatcgagga gggcgacgcg gaagcggtgg ccgcggtgct ggagcggccg
    15601 cggccggccg cggtggcgct gcgcgcaacg caaggcgccc cgtcggcgat cgcggtctcg
    15661 gcgggggacg ggcgcgccgt ccacgccccc ctcgacggct cgctgcccgg cccgatccgg
    15721 gcgtggctgg aggaccccga ccggcccatc tgggtacacg acgccaagga gctccagacg
    15781 gcgctgctgc cgatcggcgt ggaggcggcc gccatcgccg tcgacacgat gctggccggc
    15841 tacctcctgg atcccgccga ggcctcctac ccgatcggag ccctctccga ccggtacctc
    15901 ggcgccgacc tggtggggga ggccagcgag ggtcccgagg aggatgggca gctcgccctg
    15961 caggaggagg accgggggcc gctggcggcg gaggcggccg cggtggcgat cctggccccc
    16021 ctgatggccg agcggctcga gcggcagggg gccgccgacc tgctccgcga cgtggagctg
    16081 cccctggcta cggtgctcgc ccggatgcag gccgccggcg tgaagatcga cgtcccttat
    16141 ctccgggaga tgtccgagtc cctcggcgac cggatgcgga cgctcgaggc cgagctctac
    16201 gagcacgccg gcgaggagtt caacctcaac tccccgcccc agctccgggt gattctctac
    16261 gagaagctcg ggctgacccc ggggaagcgg acgaagaccg gctactcgac cgacgccgcc
    16321 acgctggaga agctgcgcca ccagcacccg atcgtggacg ccctgctctc gtaccgggag
    16381 ctggccaagc tgaagtccac gtatctcgat gcgctcccac ccctcgtgag cccggccacg
    16441 ggccgcgtcc acaccacctt cagccaggtg gccgcggcga ccggccggct gtcgtccttc
    16501 aacccgaacc tccagaacat ccccgtccgc ggggagcagg ggcgggagat ccgcaaggcc
    16561 ttcatcgccg aggagggccg ggtcctgctc gtggccgact actcgcagat cgagctgagg
    16621 gtcctggcgc acctctccgg agacgaggcc ctcggcgcgg cgttcgccga ggacgccgac
    16681 atccacgcgg ccacggcggc gaaggtgttc ggcctgccgc ccgagcaggt cgactcggag
    16741 ctgcgccgcc gggccaaggt cgtcaactac gggctagcct acgggatgaa cgcctacggg
    16801 ctcgccaccc gcctggggat cccccccgac gaggccaagg agttcatcga cgcctatttc
    16861 gaggggttcc cccgcgtgcg ggagttcctc gatcaccagg tgacgatggc cgcggcggag
    16921 ggattcaccc agacgatcct gggccggcgg cgctacctcc ccgagctgca gtcgccgaac
    16981 ccgagggtcc gcgacctggg ccgccgcatg gcgctgaacg ctcccatcca gggctccgcg
    17041 gccgacatca tcaagctggc gatgatccgc gtggaccagc gggtcgtcga gctgccggcc
    17101 acgatggtgc tcacggtcca cgacgagctg gtcctggagg tggaggaggc gtcggtggag
    17161 gaggtggccg gagccgtccg cgaggagatg tcctcggcct acgagctctc ggtgccgctg
    17221 cgggtggacg tggggagcgg gcccacgtgg gccgatgcgg tcccggaggg ccactgacgc
    17281 attgacggca tcttcgcagg tcccctaaga tgttcgggcc cttcggggcg cagtctgcgt
    17341 gcccggatcc ctcacctcga tccggttccg cgtgagcgcc gctgcgcagg gcggagagga
    17401 gctgatcgga gatcgaagag acgaaccaga gagagggcgt cgccacggac caggacgacc
    17461 tgacgcccga ggagctggcc aaagccatcg aggaatccgt caaggacttg aacgacgggg
    17521 acctcgtgac ggggcaggtg gtccggattg accccgacga ggtgctcctg gacatcggct
    17581 acaagtccga gggggtgatc cccacgaagg agctctcgat ccggcacgat gtggatccct
    17641 ccgaggaggt ctcgatcggc gacgagctcg aggcccttgt cctccagaag gaggacaagg
    17701 aggggcgcct catcctctcc aagaagcgtg cgcagtacga gcgggcctgg ggccgcatcg
    17761 aggaggtcat ggagtccggc ggcacgatcg agggccccgt gatcgaggtc gtcaagggtg
    17821 gcctcatcct cgacatcggc ctccgcgggt tcctgccggc ctcgctcgtg gatctccgtc
    17881 gggtgcgcga cctgcatccg ttcgtggggc aggtcctcga gtgcaagatc atcgagctcg
    17941 accgcaaccg gaacaacgtg gtgctgtcgc ggcgggcgtt cctggaggag tcgcagagcg
    18001 agagccgcaa gaaggtgctc gagtcgctcc agccggggga gcggcggacc ggcaaggtct
    18061 cgtcgatcgt gaacttcggc gccttcgtgg acctgggcgg cgtggacggc ctggtgcacg
    18121 tctccgagct ctcctggcgt cacgtcgagc atcccggcga agtcgtctcc gtgggcgagg
    18181 aggtcgaggt cgaggtcctc gacgtcaacc tcgagcggga gcgcgtctcg ctctcgctga
    18241 aggccaccca ggaggacccc tggaaggagt tcgagcggaa gtacaacgcc ggcgaggtga
    18301 tcgagggcga ggtcacgaag ctggtgccgt tcggcgcctt cgtgcgcgtg gcggacgaca
    18361 tcgaggggct ggtccacatc agtgagctct ccgacgcgca cgtcgagtcg gccgacgagg
    18421 tcgtgagcgt cggcgaccgc gtgcgggtca aggtcgtaga cgttgacgta tcccggcggc
    18481 ggatctcgct ctcgatgcgg cgggtcgagg gcgacgccat catcacgccg gagttcgagc
    18541 cggagcccga cgaggtcgtg cccgaggtcg aggcggaggc cgaggcggag cccgaggaga
    18601 cggtcgagga ggcaccccag ccccaagctg aggcggaggc ccaggccgag gccgctccgg
    18661 aggaggcgga ggccgagccg gagatcacgc ccgaggagac cccggccgcg gaggacgcgg
    18721 gccaggacga agaggacgtc tccctcgagg acatcgtggc cgacctccgg cgccgcgaag
    18781 gccgccagta ggcgtctccc catgctcctg gtcggcctta cgggcggcct cggagccggc
    18841 aagtccaccg tggctcgcat gctggcggag cggggcgccg tgatcgtgga cgcggacgag
    18901 ctggcgcggc ggtcgatcga gccggggacc cggggtcacc gcaaggtgct ggaggtcttc
    18961 ggggacgagg tcgaggcccg gggcggctcg atcgaccgcc gcgccctcgc cgacgtcgtg
    19021 ttcgaggacc ccgaggccag gcgagcgctc gagtccatca cccaccccga ggtcttccgg
    19081 atgcttgccg aggaggtgga gcgccggcgg gacagcggcg acgtcgtcgt gttcgacgct
    19141 ccgctcatcg tggagaccgg catggacacg gccatggacc tgctcgtggt ggtggcggtg
    19201 gctccggaca cgcaggtgga ccgcgtcgtg gcccggggga tggaccgggc cgaggcggag
    19261 gcccgtatcg cggcccaggc ctcgcaggag gagcgggagg cgaaggcgga cgtgatcctc
    19321 cggaacgacg gcgacctgcg ggacctggag cggcaggtgg atgccctctg ggatcgcctg
    19381 cgccgcgagg cagggggctg agggcggcta tcatcgcgcg gatggagacc aaagccgtgt
    19441 tcttcgacgc gggggagacc ctcgtccacc cccacccctc gttccccgag ctcttcgccg
    19501 aggtgctgag ggaccacggg cacgaggtcg accccgagac ggtgcaggag ctggccgtcg
    19561 gggcctccca gcgcttcacc gagacgctgc gcagcgacga gcacgcgctc tggtccacct
    19621 cggccgatcg ctcgcgccgg ttctggttct ccatgtacat ggggctgctg ggcgacacgg
    19681 gcatcgagga ccgcgacgga cggctcaccg acgcgctgta cgctcggttc agcgacctcg
    19741 agagctaccg gctccacccc gacgcgctgc cgctgctgga gcgcctcgag gccggcggct
    19801 atgccatggg ggtcatctcc aacttcgagg agtggctgga gggcctcctg gagaggctgg
    19861 cggtcgcgcg cttcttcccc gtgcgcgtcg tgtcgggcat cgagggcgtg gagaagcccg
    19921 acccggccat cttccggatc gccctggacc ggatgggggt ggcggccgag gcgtccgtct
    19981 acgtcgggga ccatccctac ttcgacgtcg aggcggcccg ggaggtgggg atgcggcccg
    20041 tgctgatcga ccgacgcggc ctctacccgg acgcgggcgg cgtgcggatc acctcgctgg
    20101 aggacctccc ggaggccgtc gggctgccgg tctgatgcag gagcggcgct tcacgttgga
    20161 ggaggccgag gcggagctgc cccatctgcg ggagctgctg ccccagttgc gcgaggcgcg
    20221 ccgtcgggtg ctggcgtcgg gggagcgggt gcgcggcatc gcccggcgga acggcggggg
    20281 acgggagggc accgactact ggcgggcgat cgaggcgctc cgccgggggg tggaggatct
    20341 ggcccagagg gggatcgtgc tccgcgacgc cgagggcggc ctcgtcgact tcccctcgga
    20401 gcgggagggc cggacggtct acctctgctg gcgcctgggc gaggaccgga tcggccactg
    20461 gcacgacgtg gacgcgggct tctcggggcg gcgcccgctc tgagcgtcgg gccgtagaat
    20521 cgcgtccatg tcttcgttcc gcgtccagac cgacctgcgc ccggccggcg accagccgaa
    20581 ggcgatcgac gagctgacgg aggggctcgc ggccggtgac gaccgcgtca cgctgctcgg
    20641 catcaccggg tcggggaaga cgttcaccgc ggcgaacgtg atcgagcgcc tgggcaagcc
    20701 caccctggtg atctcgccga acaagtcgct ggccgcccag ctcgcgaacg agttccgcga
    20761 gttcttcccg gacagcgccg tggagtactt cgtctcctac tacgactact accagccgga
    20821 ggcctacgtc ccgcagaccg acctctacat cgagaaggac gccacggtga acgaggagat
    20881 cgaccggctc cggcacgcgg ccaccgccgc cctcctgacc cggaaggacg tcgtcatcgt
    20941 ggccagcgtc tcctgcatct acggtctggg ctcgcccgag gaatacgagc agcagatcct
    21001 ccacgtggtc cggggacgcc cgctgggcca cacggccgcg ctccagcgcc tcgtggacat
    21061 gcagtaccag cgcaaccagg ccaacctcgc acgggggacg ttccgcgtgc agggggatac
    21121 gctcgagatt cacccctcgt accaggagac cgcgctgcgg atcgagctgt ggggagacga
    21181 ggtggagcgc gtcctggaga tggacctcat caccggcgag gtggtccgcg agctggacca
    21241 cgtgacgatc ttccccgcca cgcactacgt cgcctcgcgg gagcgcctgg atcgcgctat
    21301 ccgcgggatc gaggaggagc tcggggtgcg gctcgagcag ctggagcgga cgggcaagct
    21361 gctcgaggct caaagactgc ggatgcggac caaccacgat ctcgagatgc tgcgcgaggt
    21421 cgggttctgc tccgggatcg agaactacgc gcggcacatc gacggccggg cgcccgggac
    21481 cgccccgtac acgctgatcg actacttccc cgacgactgg ctctgcatca tcgacgagtc
    21541 gcacgtcact gtgccacagc tccacgcgat gtacgagggc gacatgagcc ggaagcgcac
    21601 gctcgtggag cacgggttcc gcctcccgtc ggccgtggac aatcggccgc tgaagttcga
    21661 cgagttcgtg gagcgcgtga accaggtgat gttcatgtcg gccacgccga gcgactacga
    21721 gctggaggtc tcgaagcggg tcgtggagca gatcgtgcgg ccgacggggc tggtggaccc
    21781 cgaggtcgag atccggccca cgcggggcca ggtcgacgac ctgatggagg aggtgcggcg
    21841 ccgagccgag gccgaccagc gcgtcctggt gaccacgctc accaagaaga tggccgagga
    21901 cctcaccgac tacctgatcg agatgggcct ccgcgtgcgc tacctccact cggagatcga
    21961 cacgatcgag cggatccaga tcatccggga ccttcgcctg ggggagtacg acgtgctcgt
    22021 gggcgtgaat ctcctgcgcg agggcctgga cctgcccgag gtctcgctcg tggcgatcct
    22081 cgatgccgac aaggaggggt tcctccggag cgagacctcg ctgatccaga ccatcgggcg
    22141 ggcggcgcgg aacgtcgagg gcaaggtgct gatgtacgcg gacgaggtca ccgactcgat
    22201 gcagaccgcg atctccgaga cccagcggcg ccgcaggatc cagctcgagc acaaccgcga
    22261 gcacggcatc gacccgcaga cgatcaggaa gaaggtttcc gacatcctcc tcaccatggt
    22321 ggaggagcgg cccgagacgg cccccaagag
//
LOCUS       DASUTZ010000099         4178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_26852, whole genome shotgun sequence.
ACCESSION   DASUTZ010000099 DASUTZ010000000
VERSION     DASUTZ010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26852"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..128
                     /locus_tag="VF097_04455"
     CDS             <1..128
                     /locus_tag="VF097_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX6262080.1"
                     /translation="IHDMGSLNGTYVNRDRVDSTQLASGDEVQIGKFKLVFFMGE"
     gene            128..838
                     /locus_tag="VF097_04460"
     CDS             128..838
                     /locus_tag="VF097_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX6262081.1"
                     /translation="MAGKEHLSIGEVLVSLKPEFPDVTISKIRFLESEGLIEPDRTES
                     GYRKFSESDVERLHEILRLQRDEYLPLKVIRDRLDRGPERNGPRPAAAGPRDEPSPED
                     EMALPATDVQLTPEEMAQAGGVDLSLIEELESFGLLSSRTVSGVRYYDGDSLVLLKIL
                     KDFFTYGVEPRHLRMYRHFADREAAFFQAIILPVLRQRNPEARKAASESLSDLARISR
                     QLKQALLRVNLREHLGNG"
     gene            853..2076
                     /locus_tag="VF097_04465"
     CDS             853..2076
                     /locus_tag="VF097_04465"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX6262082.1"
                     /translation="MRRIVGATLAAILAAAAPAAGQTGPPPTPVPPFGSPSPYPQSLE
                     TPAPSARPPRIAAPSAVLVDLSTGHVLWERRPDRPRPIASLTKIMTVLIALESSNLDD
                     VVTVSERATDEEGSELGLEAGERISVEDLLYALMLQSSNDGAIALAEHFGGSVDRFVR
                     MMNRRARELGLESTEFSSPHGLEDRGLSTARDMAALTTEAFTHPAFVRIARTKVRAIP
                     AAEGPPRRIQSRNAMLWLYPGSVGVKTGFTSAAGFCLVAAAERDGRRFAAVILGGPSA
                     AFSDAATVLDHGFFTWRVRPVVEEGAQLQPVSIDGREVPVETGGTIEGLVRRGQGAVL
                     RSLLDPSAGLPVRAGDVVGEVAAIVEGQEVGRAPLVAAEDVGSPAEDRALPPWWDDLL
                     DSIASTVPRWLETIL"
     gene            2107..2694
                     /gene="hpt"
                     /locus_tag="VF097_04470"
     CDS             2107..2694
                     /gene="hpt"
                     /locus_tag="VF097_04470"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX6262083.1"
                     /translation="MLRAMEEGARVLYREDAIQHRVRELAAEISRDYAGRDPILVSVL
                     KGGAWFLSDLIKATTINCRVDFMSISSYGGAEESMGVVRILQDLDLDIGGHHVIMVED
                     IVDTGLTLSYLLSTLSSREPASLAVCALLDKSVRRIAPVEIRYRGFDCPDRFVVGYGL
                     DHDERYRNLRCILQVEPEELGRDPLALVPYLDRPA"
     gene            2740..3219
                     /locus_tag="VF097_04475"
     CDS             2740..3219
                     /locus_tag="VF097_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017604907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEX6262084.1"
                     /translation="MVEMTLVGVRVELPANQPIVLLRETEGQRYLPIWIGAAEATAIA
                     LAMQGVTPMRPMTHDLFKNVLDELHVALEKIEITELQEGTFYAMLHLQRNGSSHEVSS
                     RPSDAIALAVRTGVPIFAAEDVLEAAAVEIHEDDAEEEVERFKQFLEDVTPEDFAEE"
     gene            3259..>4178
                     /locus_tag="VF097_04480"
     CDS             3259..>4178
                     /locus_tag="VF097_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262085.1"
                     /translation="MADDQAAFEALQAKLVPLWSSIESFNPDPQTIVVVPALPPGILA
                     GSKVQAYEERYLFLLLLLRQPNARMIYVTSMPIDPLILDYYLGLLPGVIPSHARERLV
                     AVAPGDGSDVSLSDKLLARPRLLGRIRSLIPDPDRAHLVPFFATERERRLALALGIPM
                     YAADPRFAHFGTKSGCRRLFAEEGVPHPAGREGVRDLDDVADAIGELRRERPGMRAAI
                     VKLDEGVSGMGNATVDLEGLPEPGTASAGDAVAERVRSMSTEVGEPVEAYAERLAGEG
                     GIVEERVEGAEVRSPSVQMRVSPLGKLEVLS"
BASE COUNT          603 a         1479 c         1484 g          612 t
ORIGIN      
        1 tcatccacga catggggagc ctcaacggca cgtacgtcaa ccgcgaccgg gtggacagca
       61 cgcagctcgc cagcggggac gaggtgcaga tcggcaagtt caagctcgtg ttcttcatgg
      121 gggagtgatg gcggggaagg aacacctgtc catcggggag gtcctcgtct cgctgaagcc
      181 cgagttcccc gacgtcacga tctcgaagat ccggttcctg gagagcgagg gtctgatcga
      241 gccggaccgc accgagtccg gctatcggaa gttctcggag tcggacgtgg agcggctcca
      301 cgagatcctg cgcctccagc gcgacgagta cctcccactc aaggtcatcc gggatcggct
      361 ggaccgcggc cccgagcgga acgggcctcg gccagcggcc gccgggcctc gcgatgagcc
      421 gtcgcccgag gacgagatgg ccctcccggc caccgacgtt cagctcacgc cggaggagat
      481 ggcgcaggcc ggcggggtgg acctgtcgct gatcgaggag ctggagtcat tcgggctcct
      541 ctcctcgcgg acggtgagcg gggtgcgcta ctacgacggg gacagcctgg tgctgctcaa
      601 gatcctcaaa gacttcttca cttacggcgt ggagccgagg cacctgcgga tgtaccggca
      661 cttcgccgac cgcgaggcgg ccttcttcca ggcgatcatc ctcccggtcc tccgtcagcg
      721 gaaccccgag gcgcggaagg cggcgtcgga gtccctcagc gacctggctc ggatctcgcg
      781 gcagctcaag caggcgctcc tgcgcgtgaa cctgcgcgag catctcggga acggctgaac
      841 gcgccccctc ggatgcgacg gatcgtcgga gcgaccctgg cggcgatcct cgccgcggcc
      901 gcgccggcgg ccgggcagac ggggccgccg ccgaccccgg tccctccatt cgggtcgccc
      961 tcgccgtacc cgcagtcgct ggagacgccg gccccgtctg cccgaccgcc gcgcatcgcg
     1021 gccccctcgg ccgtcctcgt cgacctctcg accggtcacg tgctctggga gcggcgaccg
     1081 gaccgccccc gccccatcgc cagcctgacg aagatcatga cggtcctcat cgcgctggag
     1141 tcctcaaatc tcgacgacgt ggtcaccgtg agcgagcggg cgacggacga ggaggggtcg
     1201 gagctggggc tcgaggcggg ggagcgaatc tcggtggagg acctcctcta cgccctgatg
     1261 ctgcagtcct ccaacgacgg ggcgatcgcg ctcgccgagc atttcggagg gtcggtggac
     1321 cgcttcgttc ggatgatgaa ccggcgggcc cgcgagctgg ggctcgagag caccgagttc
     1381 tccagcccgc acggactcga ggaccgaggc ctctccaccg cgcgagacat ggcggcgctg
     1441 acgaccgagg cattcaccca cccagccttc gtgcggatcg cacgcaccaa ggtccgggcg
     1501 atcccggcgg ccgagggccc gccgcgccgg atccagagcc gcaacgcgat gctctggctc
     1561 tatcccggga gcgtcggggt caagaccggg ttcacctcgg cggccggctt ctgcctcgtg
     1621 gccgcggccg agcgagacgg ccggcgcttc gcagcggtca tcctcggcgg gccctcggcc
     1681 gccttctccg acgccgcgac ggtcctggat cacggcttct tcacgtggcg ggtgcgccct
     1741 gtggtcgagg agggcgcgca gctgcagccg gtctcgatcg acggccgcga ggtccccgtc
     1801 gagacggggg gcacgatcga ggggctggtc cgccggggac agggggccgt gcttcggtcc
     1861 ctgctcgatc cctcggccgg tctgcccgtc cgggccggcg acgtcgtcgg cgaggtggcc
     1921 gcgatcgtgg aggggcagga ggtcggccgg gccccgctcg tggccgccga ggacgtgggg
     1981 agcccagccg aggaccgcgc cctcccgccg tggtgggacg acctcctcga ctccatcgcc
     2041 tcgaccgtcc cgcgatggct cgagaccatc ctctgaggct ccacgggtcg ggccggaggc
     2101 gcgggtatgc tacgggcgat ggaggaggga gcgcgcgtcc tctaccggga ggacgcgatc
     2161 cagcatcggg tccgggagct ggccgcagag atcagccgcg actacgcggg ccgggaccct
     2221 atcctggtct cggtgctcaa gggaggcgcc tggttcctgt ccgatctcat caaagccacc
     2281 acgatcaact gccgcgtcga cttcatgtcc atctcctcct acggcggtgc cgaagagagc
     2341 atgggcgtcg tccggatcct ccaggacctc gacctcgaca tcgggggtca ccacgtgatc
     2401 atggtcgagg acatcgtgga cacgggcctc accctctcgt acctgctctc gacgctgtcg
     2461 tcgcgggagc ccgcctcgct cgcggtctgc gccctgctcg acaagtcggt gcgccggatc
     2521 gcgccggttg agatccgcta ccggggcttc gactgtcccg accgcttcgt cgtcggctac
     2581 ggcctcgacc acgacgagcg gtaccggaac cttcgatgca tcctccaggt ggagccggag
     2641 gagctcgggc gcgacccgct ggccctcgtg ccctacctcg atcgccccgc ctgacggagc
     2701 ggttgccgct ctgcgggact ggggatacag tcgagaggca tggtcgagat gacgctcgtc
     2761 ggggtccggg tggagctgcc ggccaaccag ccgatcgtgc tgctgcgtga gaccgagggc
     2821 cagcgctacc tcccgatctg gatcggcgcc gcggaggcca ccgcgatcgc gctggcgatg
     2881 cagggcgtca ccccgatgcg gcccatgacc cacgacctct tcaagaacgt cctggacgag
     2941 ctgcacgtgg ccctggagaa gatcgagatc accgagctcc aggagggcac cttctacgcg
     3001 atgctccacc tccagcgcaa cggctcgagc cacgaggtct cctcccgccc cagcgacgcg
     3061 atcgcactgg ccgtccgcac cggcgtcccg atcttcgccg ccgaggacgt gctcgaggcg
     3121 gccgccgtgg agatccacga ggacgacgcc gaggaggagg tcgagcgctt caagcagttc
     3181 ctcgaggacg tgaccccgga ggacttcgcc gaggagtagg ggctggcccc gggcgcggcg
     3241 gacggcctat catcggccgt ggccgacgac caggcggcgt tcgaggctct tcaggccaag
     3301 ctcgtcccgc tctggagctc gatcgagtcc ttcaaccccg acccacagac gatcgtcgtc
     3361 gttcccgcgc tgccgccggg gatcctcgcc ggctccaagg tccaggccta cgaggagcgg
     3421 tacctgttcc tcctcctcct gctgcgccag ccgaacgcgc ggatgatcta cgtgacctcg
     3481 atgccgatcg accccctgat cctcgactac tacctcggtc tgctgcccgg ggtgatcccc
     3541 agccacgcga gggagcggct ggtcgccgtg gccccgggcg acggctccga cgtctcgctg
     3601 agcgacaagc tcctggcgcg accccgtctc ctcggccgga tccggagcct gatccccgat
     3661 cccgaccggg cccacctcgt gccgttcttc gccacggagc gggagcggcg cctggccctg
     3721 gccctgggga tcccgatgta cgcggccgac ccccgcttcg cccacttcgg gacgaagagc
     3781 gggtgccggc gcctgttcgc ggaggagggc gtcccacacc cggcaggcag ggagggcgtc
     3841 cgcgacctcg acgacgtggc cgatgcgatc ggggagctcc gtcgggagcg gccgggcatg
     3901 cgggccgcga tcgtgaagct cgacgagggc gtctccggga tgggcaacgc caccgtcgac
     3961 ctggaggggc tgcccgagcc cggcacggcg tcggccgggg acgccgtggc cgagcgcgtg
     4021 cggtcgatgt cgacggaggt cggcgagccg gtcgaggcgt acgccgagcg cctggcgggg
     4081 gaggggggga tcgtcgagga gcgagtggag ggcgccgagg tccggagccc gagcgtccag
     4141 atgcgcgtgt cgcccctggg gaagctcgag gtcctctc
//
LOCUS       DASUTZ010000100         2043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1062734, whole genome shotgun sequence.
ACCESSION   DASUTZ010000100 DASUTZ010000000
VERSION     DASUTZ010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062734"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1378)
                     /locus_tag="VF097_04485"
     CDS             complement(<1..1378)
                     /locus_tag="VF097_04485"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6262086.1"
                     /translation="MTSTLTRRYLAEYARRPLNLVLLLVVPAVFVALSAGVVADFAEL
                     LGGDAGGAALEAVTAGWAAAFLAGMAGFFHVGSSRGADRRLALAGMSTRRILAGRLAS
                     GLALALVAASGALGALALRTGIGDVPRTVGATAMFSVVYLGIGAAVGAVIRDEVNGSL
                     AILFVWILDVFLGPAMAGGDVLVTRLFPTHFVTLVMTDAATGHAGPLRDLGAALIWTT
                     GALALATATFAATTRPRRDRIRAGGHGTFARVAAGLRHGFREYRRSPVLWVLLVAVPV
                     VLITLSFAVTPDTPTPLESGSVTRIVSMAELHGAIMVPITVGFLAGLAGLFVVLGSAQ
                     ADRRLVLAGFRPVEVLAARLGIVAFAALLTTTASLAVTAVDFRPRAWLTFIGASLLVA
                     LTYGMLGVLAGPAVGRLGGLFLMFLVPMVDVGIAQNAMFGPTVPNWGRFLPAHGAVQV
                     LLDGAFSGG"
     gene            complement(1375..2019)
                     /locus_tag="VF097_04490"
     CDS             complement(1375..2019)
                     /locus_tag="VF097_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6262087.1"
                     /translation="MSPDEPLLRADGIVKTFRRGFGPWRRVLRVLRGAGLRLDPGEVV
                     GLVGENGSGKSTMMKILSGALSHDGGEVRRLGRIGYCPQEPILYERLTCNEHFELFGR
                     AYGLARSELDVSRTSIYETLGFGSWAGARVEELSGGTRGKLNLGLALLADPEVLLLDE
                     PYAGFDWETYQRFWALAEDRKASGRAILVISHFVTDEDRFDRIYHMENGRSVER"
BASE COUNT          369 a          738 c          697 g          239 t
ORIGIN      
        1 gccccccgga gaaggctccg tcgagcagga cctgcacggc gccgtgggcc ggaagaaacc
       61 tcccccagtt cgggaccgta ggcccgaaca tcgcgttctg ggcgatgccg acgtcgacca
      121 tcggcacgag gaacatgagg aacagcccgc cgagcctgcc caccgccgga cccgcgagca
      181 cccccagcat cccgtaggtg agcgccacca gcaggctcgc tccgatgaac gtgagccaag
      241 ccctcggccg gaagtccacc gcggtgacgg cgagggaggc cgtggtcgtc agaagggccg
      301 cgaacgcgac gattccaagc cgggcggcca gcacctcgac ggggcggaag ccggccagca
      361 ccagccgccg atccgcttgg gcggacccaa gcacgacgaa caacccggca agcccggcca
      421 ggaagccgac ggtgatcggg accatgatcg ccccgtggag ctcggccatg gacacgatcc
      481 tggtcaccga gcccgactcc agcggcgttg gcgtgtcggg cgtaacggca aagctcaggg
      541 tgatcagcac caccggaacc gcgacgagca gcacccagag caccgggctc cggcggtatt
      601 cccggaagcc gtgccgcagg ccggccgcca ccctggcaaa cgtgccgtgt ccaccggcgc
      661 ggatccgatc cctcctgggc cgggtagtgg cagcgaaggt cgcggtggcc agggcaagcg
      721 ccccggtcgt ccagatcagc gcagcaccga gatcgcgcag cggaccggcg tgcccggtgg
      781 cggcgtcggt catcacgagc gtgacgaagt gagtggggaa caatcgggtc accaacacat
      841 cgcctccggc catcgccgga ccgaggaaga cgtccaggat ccagacgaag aggatggcga
      901 gcgagccgtt gacctcgtcg cggatgacgg cgcccaccgc ggccccgatc cccaggtaca
      961 cgacggagaa catcgccgtc gcgcccaccg ttcgggggac gtccccgatg ccggtccgca
     1021 gggccagggc ccccagcgcc ccgctcgcgg cgaccagcgc caacgcaagc cccgaggcca
     1081 ggcggccggc gaggattcgc ctggtgctca tccctgcaag ggccaggcgg cgatcggctc
     1141 cgcgcgagct cccgacgtgg aagaagccgg ccatgccggc aaggaaggcg gctgcccacc
     1201 ccgcggtgac ggcctcgaga gctgctccgc ccgcgtctcc ccccaggagc tcggcgaagt
     1261 ccgcgaccac acccgccgac agagccacga agaccgccgg aaccaccaac aggaggacga
     1321 ggttgagcgg gcggcgggcg tattcggcga gatagcgacg ggtgagtgtg gaggtcaccg
     1381 ctccacgctc ctcccattct ccatgtggta gatacggtcg aagcggtcct cgtcggtgac
     1441 gaagtggctg atgaccagga tcgcgcgccc cgaggccttc cggtcctcgg cgagcgccca
     1501 gaaccgctgg taggtctccc agtcgaaccc ggcgtacggc tcgtcgagga ggagcacctc
     1561 gggatcggcc aggagcgcca gcccgaggtt gagcttcccc cgggtgccgc ccgacaactc
     1621 ctcgacccgg gcgcctgccc acgagccgaa cccgagggtc tcgtagatgc tcgtccggga
     1681 cacgtccagc tcgctgcgcg ccaagccgta ggcacggccg aacagctcga aatgttcgtt
     1741 gcacgtaagg cgctcgtaca ggatcggctc ctgtgggcag tacccgatcc tccctaggcg
     1801 gcggacctca cccccgtcgt gggagagcgc gccggagagg atcttcatca tcgtgctctt
     1861 gccggacccg ttttcgccca cgagtccgac gacctccccg ggatcaagac gcagaccggc
     1921 gccgcgaagc acgcgcagga cccttcgcca gggcccgaag ccgcggcgga aggtcttcac
     1981 gattccgtct gcccggagca ggggctcgtc cggtgacagg ccgggaccgc cgagattccc
     2041 gga
//
LOCUS       DASUTZ010000101         3185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_415289, whole genome shotgun sequence.
ACCESSION   DASUTZ010000101 DASUTZ010000000
VERSION     DASUTZ010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415289"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..74)
                     /locus_tag="VF097_04495"
     tRNA            complement(2..74)
                     /locus_tag="VF097_04495"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39..41),aa:Arg,seq:cct)
     gene            323..3046
                     /locus_tag="VF097_04500"
     CDS             323..3046
                     /locus_tag="VF097_04500"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX6262088.1"
                     /translation="MKTLIDALAAVVRRAPWVVIVVTLAITAFLGYQNQNFQPAEDQN
                     EAFAPEAPELAAAERIDELFGDESITSPMQVVIKGGDVITADGLAAVQAVTETMLSGS
                     LSEFLVEATPEQPSSVSFLSPVLAAVEAGAPPPTTDEEVKALYREGLDALPPEQAGFI
                     EQLLPTEVDASGASSPSGLVITFYTGAASNDEFTEYAEASSDAAREIRGIPMPPGLTA
                     EPFSFELLFEGDDEFQAEIGRLFATAAFIILFVLSLVFLMRPRSGRDTVLRAVGIVGM
                     LLALVVLIIPGLALVLPDLFPDSFQDLETGPVLGIAALVYLVIFVLWVFLSPRALRRT
                     TANAFLTMLTILVAITWMNGYGGLRFGSASPMAQILPILLIGLGVDYAIHMNSRYQEE
                     LASGASVDRGVMTAIRTVGIALVLATITTAVGFLTNVTNEIPALREFGELAAFGIVTS
                     FLLMLTFVPAIRELLDRRGERRETLDRAALQAGGDRALPRLIGRAAALPLRAAVVVVI
                     VSIVLAVVGAFGVTRLEAKFSIVDFVPTTSPVRGTFETLLEDFGGGFGETTQILVEGD
                     VATPEAYNAMVQTTANLGDTENVLTFGDMPVVESPVSLVTSLSIPDTPSFDPGVAALA
                     AEAGISAEDPTVAPGADVAALYDAASAARPDIAATVLGDGGSYEAALFTIQTQAGEAG
                     ATQLREDLAVDMVPLASAGVDATATSDEIIGDVIIITLRDSQVSSLLITLAAALLLLV
                     VNFWVTSRRPMLGVITTVPVILVVLLSFTLMVAFGIAFGPVTATISALAIGIGIPYMI
                     HITHRYLEDRARHPSDGEAIGKTLVHTGGALAGSALTTIAGFGILVISTTIPFRQFGF
                     VTAYTIGLALLAAVLILPSYLVLWDRWHRRRGEDPVDREAVEAALRSGVGS"
BASE COUNT          431 a         1091 c         1126 g          537 t
ORIGIN      
        1 gcgcccccgg gaggaatcga acctccgaca cccggtttag gaaaccggtg ctctatccac
       61 tgagctacgg gggcgggggc gatcctcccc aagtctagcg ggtgggaaag ccacgcccag
      121 gcggggcggc atccctcggc ccgcgccgcc cacgccggtc gtctcctgag caagcgatga
      181 gttcggcgcg ccttcggggg tcgaagttcg gggagaacgg cgggggaatc gaaagcgccc
      241 ctggtctgtt cgagatgtga gaggcttggc ggccggcagg tcgcgtgtcg tcgggccgta
      301 cgccacagag agagggaggc tcgtgaagac gctcatcgac gccctggctg ccgtcgtgcg
      361 ccgggccccg tgggtcgtga tcgtggtcac gctggccatc accgcgttcc tcggctacca
      421 gaaccagaac ttccagccgg cggaggatca gaacgaggcc ttcgcgccgg aggcgccgga
      481 gctcgctgcc gccgagcgga tcgacgagct cttcggtgac gagtccatca cgagccccat
      541 gcaggtcgtg atcaagggcg gggacgtgat caccgcggac ggcctggcgg ccgtccaggc
      601 cgtcacggag acgatgctct cgggctcgct ctccgagttc ctcgttgagg ccacgcccga
      661 gcagccgagc tccgtcagct tcctctcgcc ggtgctggcg gcggtcgagg cgggggcgcc
      721 cccgccgacc accgacgagg aggtcaaggc gctgtaccgg gaggggctgg acgccctgcc
      781 tccggagcag gccggcttca tcgagcagct gctgccgacg gaggtcgacg cgtcgggcgc
      841 aagctcgccc agcggcctgg tcatcacgtt ctacacgggt gccgcctcga acgacgagtt
      901 caccgaatat gcggaggcct cctcggatgc cgcccgcgag atccgcggga tcccgatgcc
      961 tccggggctt accgccgagc ccttcagctt cgagctgctg ttcgagggcg atgacgagtt
     1021 ccaggccgag atcgggcggc tgttcgccac cgccgcattc atcatcctgt tcgtcctctc
     1081 gctggtgttc ctgatgaggc cgcgctcggg tcgcgacacg gtcctgcgcg cggtcggcat
     1141 cgtggggatg ctgctcgcgc tcgtggtgct gatcattccg gggctggcgc tcgtgctccc
     1201 cgacctcttc ccagactcgt tccaggacct cgagaccggc ccggtgctgg ggatcgcggc
     1261 gctcgtgtac ctggtgatct tcgtgctgtg ggttttcctg tccccgcggg cgctgcgccg
     1321 gacgacggcg aacgccttcc tcacgatgct cacgatcctg gtcgcgatca cctggatgaa
     1381 cggctacgga gggcttcgct tcgggtccgc cagcccgatg gcgcagatcc tcccgatcct
     1441 gctgatcggc ctgggcgtcg actacgcgat ccacatgaac agccggtacc aggaggagct
     1501 cgcgagcggc gcgtcggttg accgaggggt catgaccgcg atccgcacgg tcggcatcgc
     1561 gctcgtgctc gccacgatca ccacggcggt cggcttcctg acgaatgtga cgaacgagat
     1621 cccggccctc cgcgagttcg gcgagctggc ggcgttcggg atcgtgacct cgttcctgct
     1681 gatgctcacc ttcgtgccgg ccatccgcga gctcctcgac cgccggggtg agcgccggga
     1741 gacgctcgat cgggccgcgc tgcaggccgg cggagaccgg gccctgccca ggctgatcgg
     1801 gcgggccgcg gccctcccgc tgcgggcggc ggtggtcgtg gtcatcgtct cgatcgtctt
     1861 ggcggtcgtc ggcgccttcg gggtcacccg tctggaggcc aagttcagca tcgtggactt
     1921 cgtccccacc acctccccgg tgcgcggcac gttcgagacc ctgctggagg acttcggcgg
     1981 agggttcggc gagaccaccc agatcctggt cgagggggac gtggcgacgc ccgaggccta
     2041 caacgcgatg gtccagacca ccgcgaacct cggcgacacc gagaacgtcc tgacgttcgg
     2101 cgacatgccg gtggtcgagt ctcccgtttc cctcgtgacg agcctttcga tccccgacac
     2161 gccgtcgttc gatccgggag tggcggcgct ggcggcggag gcgggcatct ccgcggagga
     2221 tcccaccgtg gccccgggag ccgacgtcgc cgcgctatac gacgccgcgt ccgcggcccg
     2281 acccgacatc gcggcgacgg tgctcggtga cggcgggtcc tacgaggccg ctctgttcac
     2341 gatccagacc caggccggcg aggcaggagc gacgcagctc cgcgaggacc tggccgtgga
     2401 catggtgccg ctggcgtccg ccggtgtgga cgcgacagcc acgtccgacg agatcatcgg
     2461 cgacgtgatc atcatcacgc tccgggactc gcaggtctcg tcgctgctga tcacgctcgc
     2521 cgcggcgctg ctgctcctgg tcgtgaactt ctgggtcacc tcccgcaggc ccatgctggg
     2581 cgtgatcacg acggtgcccg tgatcctggt ggtgctgctc tcgttcacgc tgatggtcgc
     2641 gttcgggatc gccttcgggc ccgtgacggc cacgatctcg gccctggcga tcgggatcgg
     2701 gatcccgtac atgatccaca tcacgcaccg ctatctcgag gatcgggcga ggcacccgag
     2761 cgacggcgag gcgatcggga agacgctcgt ccacaccggg ggcgcgctgg cggggtcggc
     2821 gctcaccacg atcgcggggt tcgggatcct cgtgatctcg accacgatcc cgttccggca
     2881 gttcgggttc gtgaccgcct acacgatcgg gctcgcgctg ctggccgcgg tgctgatcct
     2941 gcccagctac ctagtgctgt gggaccggtg gcaccgcagg cgcggcgagg acccggtgga
     3001 tcgtgaggcc gtcgaggcgg cgctccgatc cggggtcggg agctgagggc ctaggatctc
     3061 ggcatgaccg acgtgcgcgc cccccggcgt ctgtggcggc ctccggcgcg gttcgaggag
     3121 cgggcgccgg accggcgcgt gaccttcctc gagctgttct tcgatctcgt cttcgtggtc
     3181 atcat
//
LOCUS       DASUTZ010000102        22171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1452607, whole genome shotgun sequence.
ACCESSION   DASUTZ010000102 DASUTZ010000000
VERSION     DASUTZ010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 22171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22171
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1452607"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VF097_04505"
     CDS             complement(<1..450)
                     /locus_tag="VF097_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014878135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX6262089.1"
                     /translation="MDDLRARVRELMPQIRADLDRLVRIPSVSAEGFDPEPVRESAKA
                     TAEVLEAAGFRDVRFLEVDGAHPAVTGRIEGPQGSPTVMLYAHHDVQPAGDPAEWTSP
                     PFEPTERDGRLFGRGAADDKAGVMAHVAAVLAHDGKPPVTVTAFIEGE"
     gene            506..997
                     /locus_tag="VF097_04510"
     CDS             506..997
                     /locus_tag="VF097_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013409200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX6262090.1"
                     /translation="MARLPLRSRPPVVALTIALLAAGCGEGPLEPAEDDLVPVVRVVD
                     GDTIWVAPEQRVRLIGIDTPEVDWYGGRAECYGEEAGLFMQGLLEGERVRLEYDQEPR
                     DRYGRTLAYVYLEDGRMVNLLLVRRGYATVSIFEPNDRHEAGLRAAEREARGVGSGLW
                     SIC"
     gene            complement(1358..2500)
                     /locus_tag="VF097_04515"
     CDS             complement(1358..2500)
                     /locus_tag="VF097_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6262091.1"
                     /translation="MDRDFGLSEDQREIVGLVREFVNERVRPQIEAFEEKGEFPREPF
                     RQLGEMGLGGIPYDEAYGGAAQPYLTYLTVLEELAMGSVSLAVGLSVHHLATFGVHDY
                     GSDVLKERYLPRLTSGEWLGAYALTEAGAGSDAASLATKADRDGDGWRLTGSKRFISH
                     GGEAEYYLVMARTGGEGPKGISAFVVEKGYPGFDFGKPEEKMGWNASPMRELLFDGAE
                     VPGENLVGEEGQGFTIALAALDGGRLGMAACGVGVAQAALDAAVAFAKDREQFGKPVI
                     EFQGLAFMLADMHAKVEAARRLYREAARARDEGVGTPVSCSVAKLFATDVAMRVTTDA
                     VQVHGGYGYMREYPVERYMREAKALQIVEGTNQVQRMIIGRHLAGG"
     gene            2562..3953
                     /gene="cysS"
                     /locus_tag="VF097_04520"
     CDS             2562..3953
                     /gene="cysS"
                     /locus_tag="VF097_04520"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX6262092.1"
                     /translation="MRLYNTLTREVEDVQPPGDGPVRIYTCGPTVYRPVHIGNLRTFL
                     LSDLVRRALELEGHEVLQVVNITDVGHMVDDSSEEALDRMELAMEDEGLGPWEIAEKY
                     TRVFLEDADKIGLLRAHEYPRATDHVAEMIEMTKTLIDEGHAYALDDGSVYYDVRSFP
                     DYGRLSRNTLDKLQPGHRDLEADDRKRHHADFALWKRAQPNRLMKWDSPWGEGFPGWH
                     IECSAMSLKYLGDRFDIHTGGVDLVFPHHEDEIAQSDGATGHRVVSTWVHGGHLRVEG
                     QKMAKSTGNVYTIDDVIEGGYDPLAFRLLCFQTRYRSEMNFTWEAMEATDRAVTRWRQ
                     RMAEWKDAPSGEPSREGEVFDRRFREAVADDLDMPRAVAVVGATVSSEIPEGEKRALL
                     SSWDAVLGLDLDRIVREGFEVPADVRSLVEERDAARAGRDYARSDEIRDRLSEMGWEV
                     MDSAEGTRVRPLA"
     gene            3965..4858
                     /locus_tag="VF097_04525"
     CDS             3965..4858
                     /locus_tag="VF097_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018722436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6262093.1"
                     /translation="MDAAILTQGLTKYYGKRPGVLEVDLEVRPGEVFGFLGPNGAGKT
                     TMIRTLMDLIRPTSGRAFVLGMDAQREGVEVRRRVGYLPGDLALDEHLEARDLLGYLS
                     SLRGGVHEDEIERLASLLDAELDARIGSLSSGNRQKIGIVQAFMHRPELVVLDEPTLG
                     LDPLIQQHVYRLLAETKADGRTVFLSSHNLPEVERTCDRVGIIRGGRLVAVEDVREIR
                     SRALREMEIHFAAPPTGNGFSRLPGVREVHVSGESVRMQVEGEVDAVVKEAAKHRVVS
                     ITTRQPTLEEIFLAFYGEEEA"
     gene            4858..5658
                     /locus_tag="VF097_04530"
     CDS             4858..5658
                     /locus_tag="VF097_04530"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6262094.1"
                     /translation="MASIFVKALRDRRRVLIWWALGMIATALMLASFYPTIRETGEDL
                     NAYIENLPEALRAIVLSGGGDFTSGPGYLNSELFSFMLPLIVLVFSVGMGAGVVAGEE
                     REGRLELMVASPVTRRRIVLQKLAAILVGMIGLGIVLWATLAVGVEIWDIPVDHANLV
                     AACAMAVFLGWVFGALALAVGAATGRRGMAIGIPAAVGVATYLVNGLAPLSDALEFWR
                     PLSPFYYYAADQPIRSGIDLGDAGILLALSVILGIAAVLAFDRRDLAT"
     gene            5655..6101
                     /locus_tag="VF097_04535"
     CDS             5655..6101
                     /locus_tag="VF097_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262095.1"
                     /translation="MSSRENGQPRWRPAIPTVLVVASIVVALVAVSALLYVLFRETVG
                     PGEVLRDFAEALSEDDCPGSYDLLDGSVRASITEEEWCDEVPGLAAKLSPRFEIERVI
                     LSGGDARVEVSGPETTTGAWFLRRQGRSWTVLGGGDAIEFPTELPT"
     gene            complement(6098..7279)
                     /locus_tag="VF097_04540"
     CDS             complement(6098..7279)
                     /locus_tag="VF097_04540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6262096.1"
                     /translation="MALLDIPEELLELRSTLRRFVEREIAPVEEAFAQQIQEGDYSFI
                     KDERAKLRKRSAELGFWGIHMPEEVGGMDLPFLGQVLLAEEAVRSGLVLAHAEAIFPI
                     VSGPSRVHLDCTPEQREKYLAPLMTADKLNCFALTEPEAGSDATRIRTRAERTDGGWV
                     LSGRKHFISAGDSADFALVFAVTDQQKGARGGITAFLVDADTPGYSVTRIQRTMSPLQ
                     NPVELTFEDCEVPDAAVLGEVGAGFYSAIKGINGARFGIAATALGTAGHLLERMKEYA
                     GSRIAFDRPIGANQYVQGMVVDSYAELEQARLLVYKLAADVDDGADGRREGALAKLVS
                     TEAVGRIADRAIQVHGGQGYMTELGLEVWYRDVRAMRLYEGTSEILRQNVAKTLGLPV
                     L"
     gene            complement(7341..8441)
                     /locus_tag="VF097_04545"
     CDS             complement(7341..8441)
                     /locus_tag="VF097_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005481973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX6262097.1"
                     /translation="MGREHTERLRRAAEATAEADLHALLVTPSADLIYLTGYDPPPLE
                     RLTCLVIRPEHDPVLVVPGLERPLAEGRLARHVTEIRSWDETEDPYELVAGVVGSKSS
                     KLGCSDRMWASHLLGIQRALPKARAVTASRVLGPLRSVKDRHELHMLARAARATDEAF
                     RRLLEERIEGATEREVAGRLGELMLELGLERVEFAIVGSGPNAASPHHDPMDRPLRAG
                     DAVVMDFGGTSGGYCSDMTRTVAVGKAPARLREVHEVVAEAQEEALRAVYPGVPAEDV
                     DRAARQVIERAGYGDRFIHRTGHGIGLEAHEDPYIVRGNRDSLRPGMCFSIEPGIYLE
                     GDLGVRIEDIVVVTEEGARRLNHAPRELAEVR"
     gene            8503..10908
                     /locus_tag="VF097_04550"
     CDS             8503..10908
                     /locus_tag="VF097_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262098.1"
                     /translation="MAWKQQRLGPRLLEAARSGDRYRDVHIVLGGTGAVGGTAVLQLL
                     SLYEEMFSVAPPGPDDVPVLVATGVTGDEISAFTRRLFRFVESRHGAEALPERVRRGY
                     LTHSGVFVALERFMVTVLPALRRLRQVPPEVRPRVIEELLAELGTTPEGAADAIAETV
                     SGARPFTSFLQAYRDEHLGGGRFRSVVVGIPIPSLIAYHQQELDEAAEHLPGLTPDRL
                     ENLKQRFISALRDDLVAVQASLADEVLIAHTTGVGGMYDPRSDGGSEIRLGFAHAALD
                     LALAEKARFAQELTDLYSQAGIKVLVTAAAIGIDEVKVRERVPLHRGVRHRLFEAPVE
                     LFPGAKASQPRESKASRRAGRPVPAAQHMRVFPPITVPFDDPPSEPARFEPGEELAPT
                     YTIRSGENGFFSVADAESLYRVMKVASASELGLLLATVGMLGDDPSRPWFEGNVCYYG
                     ETDNGRQVFDFLSQPTLRRTQLSGLEPMSLQDLGSAKHQGELHTLALLILLHRLRTLD
                     VDAIDPYVDLERFDPRAFFEQRSRTLTFEQLALWEPEELVRDMQALAAAETLDDLLPL
                     APHREHELFPRRAEAVRRVLGEVLKAVWTGPALGLPILYERDGVSHLRCGYYAAPLDL
                     LVTDTDAVAVWMRKAHAESGNPAPFEDFRDYHLAVGGFVDVRPHAIVCAAKSDREDLT
                     GKVARFEDEGRLREHLRTLEPYSFFSTCGLVAVLHRLRALYGLLRESMLELGTLQEWR
                     WSMPRDAAGHMVVLPGAAEALRMAAEGLEKSTGAERLDGPWGYERRPVPDRRGTIPGV
                     HDR"
     gene            complement(10905..11819)
                     /locus_tag="VF097_04555"
     CDS             complement(10905..11819)
                     /locus_tag="VF097_04555"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX6262099.1"
                     /translation="MPSPFGPLVLIVNPRAGRGEVGREMPELERRIVAGRLEYRIEET
                     QKPGDASRLAREAVESGERFVVAVGDDATVNEVVNGLMDGDGPIDEDLVVGAMAAGSG
                     ADFVRTFGLPDQVTTGARHLLGEHTYPIDVARITYGPEGRSRYFVNAAQVGLGGLAVR
                     RAAALPAWLGRGRQFLGFWRSVIGYRVSNLRVRADRREFEGRAHGVVIANGQFLNGGM
                     RVSPRSYPGDGKLDVQVSIGPKSQAFTILPSIYRGEHLPDPQIKEMLGAEIVVESDRP
                     IPVEADGVPLGETPVSVTVLREALRLKI"
     gene            complement(11822..14470)
                     /locus_tag="VF097_04560"
     CDS             complement(11822..14470)
                     /locus_tag="VF097_04560"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX6262100.1"
                     /translation="MDPERFASRYPFPLDDYQRRAIEALALGESVLVAAPTGSGKTVV
                     AEYAIEQALSRGRKAFYTTPLKALSNQKYGDFVARYGASEVGLLTGDNSINSEASIVV
                     MTTEILRNMLYQQSPTLRDLEVVVMDEVHYLQDPYRGPVWEEILIHLDLVVSVVALSA
                     TVSNAEEFGEWLTTLRGATRVIVEERRPVPLEHLYMTGRELHPMHVEEDGHLAPNPYL
                     VSLSQRELRVKEYRRRDGRRHYERRGRPREGHRRVYVPRREEVIRVLEREGILPAIYF
                     VFSRAGCDRSVEYAMEAGIRLTSPEEQERIREFVEMRTAWMDEEDLQSLGFYELREAL
                     TAGVAAHHAGMLPVFKETVEELFARGLVKIVFATETLSLGINMPAKTVVIEDLFKFTG
                     ERHEVLTPGEYTQLTGRAGRRGIDDLGYAVVLHQRQVEFERVAGLASQRTYELTSSFR
                     PSYNMTVNLVRNYTLEEARHLLNSSFGQFQADRAVVALERQAERDRAGLAGYAEQLAC
                     DRGDFEEYWGMLEEAQELRREADRAEERRRQEEIKDALGRLRPGDVIFVPRARRRGLA
                     VVLANREGRPSVLTQDRKAFRATPRDFQDPPESLARVQLPKRGSARSARYRRDVAAKL
                     VSMNVRPPKRRRGGPQREALSRAEELEARARKHPVASCPDLKEHERWARRASKLRRRV
                     RGLERRIRARTDSLARQLDRVLEVLTSLGYVSDFSLTPKGERLTRIYGEGDILVAEAI
                     EDGVLDVLTPAEVAAVVSTVVYESRERTPRSPTLPTVEVESRFGRLRSLYLEVRRAEE
                     RAGVELCRELDAGFAEPAFHWAEGKALEDALRETEMAPGDFVRTCKQLIDLLRMIEDV
                     ADEDLAALVHEARTAVNRGVVAYTGL"
     gene            complement(14497..15222)
                     /locus_tag="VF097_04565"
     CDS             complement(14497..15222)
                     /locus_tag="VF097_04565"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6262101.1"
                     /translation="MTDLVLIHAFPVDAAMWGRQVADLAGEARTLAPHLPGFGGEDLP
                     DGPLTVSGMADAVAAAMDRVGIDRAVIGGCSMGGYAAFEMWRRHRDRVAGLLLVDTRA
                     EPDDEEGRAKRRDLAQRVREEGSGVVVESPPPLLSAEASGTLWEEVKAWIARQPAEAI
                     ALASLGMAERPDSRPDLSGIDVPTTVVVGSGDTLTPPPMSEAMAAAIPDAELVVLDGA
                     GHLSNLEDPEGFLEAARGLLRRA"
     gene            complement(15219..16100)
                     /locus_tag="VF097_04570"
     CDS             complement(15219..16100)
                     /locus_tag="VF097_04570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX6262102.1"
                     /translation="MIVLVANPSSGGGKGGRVMPRAIRALGSLGRAYRVVVTEDGSHP
                     QRAAREALDAGAEAVVALGGDGLVSAVANALIGTGVPLAVVPTGAGNDFARRLGLDHR
                     RPLRSIALLDRGARRTVDTVRMEGAGWTRSYVCVAGAGFDSETNEVANRMRLLTGTVK
                     YVVALMRTLRTFEPAEFRVVTDDEDLRLRAMMVAVANAASYGGGMMVCPDAKMDDGLL
                     DLCVVGAMSRRSFVAAFPSVYRGTHVTHRDVTMLRSRKVALEASRPFDVYADGERFGP
                     LPVTLTVEPRTLEVIAP"
     gene            complement(16105..16860)
                     /locus_tag="VF097_04575"
     CDS             complement(16105..16860)
                     /locus_tag="VF097_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262103.1"
                     /translation="MAIKGKKRSRGGRSRARAVAPRPTLVVPKKPFFQRTWVRWSLIG
                     LLVLTVSGLAFGVIAARRAAAEREAAQNEVERVGEELERAVAPVAQVSGQSLVVLPQL
                     GATLLDIQAGEARERRVRRQIARWEEDLAATRDDLGAIETDRRDLRRSIERISGGLEF
                     FQVLLQGVPALLDEEQEARVAEATVALQGDLQTAAGTVQSGYLIYLNERVDVELDEGT
                     PGLQTELPGNNPFGVPGPGEVPIPEAPAPEPTG"
     gene            complement(16870..17601)
                     /gene="tatC"
                     /locus_tag="VF097_04580"
     CDS             complement(16870..17601)
                     /gene="tatC"
                     /locus_tag="VF097_04580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX6262104.1"
                     /translation="MSVVEHLEELRTRIFVALVAILVMSVIGFVFYQDILEIILRPYR
                     RALATLPPGAIPGGALEGRLVYSSPTDPFITFLKVGFFSGLMLALPLILYQVWAFVTP
                     GLTKRERRLGAPFVLLSVALFAGGAVFAYAIIPRGLRFLLGFGGESLVPLLTVDRYVG
                     FIIMVVLAFGLSFEFPLLMMFLAAARVITSDQMRTWRRYVYLGLVVFAALVTPTQDPY
                     TMLLMTVPLILFYEAAILVARFFKR"
     gene            complement(17677..17961)
                     /locus_tag="VF097_04585"
     CDS             complement(17677..17961)
                     /locus_tag="VF097_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017870571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX6262105.1"
                     /translation="MPNLGFGEIIVILLVALLVFGPRRLPEIGRTVGKSLREFRRATT
                     NLKAEFEQDLDVGPPRRTPARRRRPPTANGETSERAPGEEPEPETGGPPE"
     gene            complement(17974..18252)
                     /locus_tag="VF097_04590"
     CDS             complement(17974..18252)
                     /locus_tag="VF097_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262106.1"
                     /translation="MIPLAFVIAVALATLLVLVVAVVSLVRQMKRVVTSIVEFQEELQ
                     PILMEIQGGADRASRRLVELQRDGVAAGSRGRPPRVVPVDPDDPLAPG"
     gene            complement(18274..18663)
                     /locus_tag="VF097_04595"
     CDS             complement(18274..18663)
                     /locus_tag="VF097_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262107.1"
                     /translation="MTTIRATCPRCGEVEMSAASVLLVVEAGTTEGTYRFVCPSCEDT
                     VEKRADRKVVMLLLSAGVEVREVSHEVVSMEHPSVRSHQPSFEEAVQEDVRVAQEEPA
                     FTLDDLIDFHFLLQQEDWFDELARSAG"
     gene            complement(18713..19651)
                     /locus_tag="VF097_04600"
     CDS             complement(18713..19651)
                     /locus_tag="VF097_04600"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX6262108.1"
                     /translation="MPPSKVARRIERLLAIVPYVIRHPGTTLEELGRLFDVDPRSLTR
                     DLNLLFMTGLPPYDPGNLIEVDIEDGRVWIGMADYFRRPVRLTPAEALALYLKGTVFL
                     GAQGLSEADALRSALDKLKEHLGETLGDLQIEAGEGKPSGPLDAVRGAVERRETLEID
                     YYSASRDEVTTRRIDPEHVFSALGNWYAVAWDHLRDGERLFRIDRIREARRTGDPFEP
                     RGLAGAGRPLYTPTDRDVAVRLRLGPRARWVSEYYEVDDEREVDGGLEVTLPTPDLAW
                     VAKLVLRVGADLEVLHPPELRETARTMTEEALARYA"
     gene            complement(19638..20588)
                     /locus_tag="VF097_04605"
     CDS             complement(19638..20588)
                     /locus_tag="VF097_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX6262109.1"
                     /translation="MEPLERLLKLVALLLNAPRPLTFDEIRDEMGAYTQEDREVAKRQ
                     FERDKDDLRRIGIPIETGGTDVYEVEVGYHIPKERYYLPEISFTPEEIAALFIAAHAP
                     GPYEQAAQAFGKLARDADTAVISGLVDRRVPGLDDSSVNVMDAADAAARRRLVRFAYR
                     PIGGEVSDREVDAWGLVSFRGAWYLVGRDRSAQDVRSFRLSRIVEGLEDIGEADAPPE
                     GFDTRDRLRSGPWGVGEPQTTATVAFAPKVAWWARDQAPGSTVVETRDDGWTVLEVPA
                     STDESFLGWVRAFGPDAELLEPEDLRRRLAESLEEIRASL"
     gene            20659..21756
                     /locus_tag="VF097_04610"
     CDS             20659..21756
                     /locus_tag="VF097_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HEX6262110.1"
                     /translation="MIRLRHGVVRSVEAHRPGLLELGVEVEGRSELAIAYPQLTGPVR
                     EGDRVLLNTTAVVAGLGSGGYHFVLAVEEAPDADPPGEGHVMKLRYTPLQAKVLAVEE
                     QDSPDREAVMDADSLDGLPVVWIPLHSMLGAACAGARAAGARRIAHVMTDGAALPIWL
                     SRQVHELREAGLLDVTITAGQALGGDREAVNVFSALLAAREVEEADVVVVGDGPGKVG
                     TATRWGATEVSGGLALQAAAILGARAIAAVRINFADPTYRHHGLSPHSLTVLSRVASP
                     GAHVAVPTLEGPRRDVVWEAIREAKLEERHQVVEVTGQPALDLLAERGVGAETMGRKL
                     EDEPEFFLAAGAAGVLAGRMAARTERWKSET"
     gene            21756..>22171
                     /locus_tag="VF097_04615"
     CDS             21756..>22171
                     /locus_tag="VF097_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262111.1"
                     /translation="MARTLEVTFNATGAAAAGARGRVVCVVDVVDSSTSAEAALSAGA
                     AAVLGAAPADADPPVTVRPDAVGMRAVALAGEHDASVLVVAEPRVGSQEERQERAAAV
                     LNGIQGAAIEWQLVPNQGAELPALASCAGRIVVVVST"
BASE COUNT         3134 a         8031 c         7794 g         3212 t
ORIGIN      
        1 ctcgccctcg atgaaggcgg tgacggtcac gggaggcttc ccgtcgtggg ccagcacggc
       61 cgcgacgtgg gccatgacgc cggccttgtc gtcggccgcc ccccgcccga acagcctccc
      121 gtcccgctcg gtgggctcga aggggggcga ggtccactcc gctgggtcgc cggcgggctg
      181 gacgtcgtgg tgggcgtaga gcatcacggt cgggctcccc tgcggcccct cgatgcgtcc
      241 ggtcacggcg gggtgggcgc cgtccacttc caggaagcgg acgtccctga accccgcggc
      301 ctcaaggacc tcggcggtcg ccttcgccga ctcccggacc ggctcggggt cgaagccctc
      361 ggccgagacc gaggggatgc gcacgaggcg gtccaggtcg gcgcggatct gcggcatgag
      421 ctcgcggacg cgagcgcgga gatcgtccat cggcgccaca gggtatcagc gtccctgtcg
      481 gtggggccac gtacgctggt gctcgatggc caggctcccc ctccgctcgc gaccgcccgt
      541 ggtcgccctg acgatcgcgc tcctcgctgc gggctgtggc gagggcccgc tcgagccggc
      601 cgaagacgac ctcgtgcccg tcgtccgcgt cgtggatggc gacacgatct gggtcgctcc
      661 ggagcagcgc gtccgcctga tcggcatcga cacgcccgag gtcgactggt acgggggccg
      721 ggcggagtgc tacggagagg aggcgggcct cttcatgcag ggtctcctgg agggcgagcg
      781 ggtccggctc gagtacgacc aggagccgcg ggaccgctac ggccgcacgc tcgcctatgt
      841 ctacctcgag gacggccgca tggtgaacct gctgttggtc cgccgcggct acgccaccgt
      901 ctcgatcttc gagcccaacg accggcacga ggccgggctg cgggcggccg agcgggaggc
      961 tcgcggcgtc ggcagcggcc tctggtcgat ctgttgactc aggcgttgac gggaggtccg
     1021 ggctcgcagg tcgcaaccac cgatccatcg tgtcgcgtct cggctgtgat ccctccactg
     1081 ctccctgcac cgaaacccgg aggcccgacg gtgacgccac ccttcccgct gcaccggatg
     1141 aggtctcctt cgcgcaactc tcctgagctg aagaccacgc gctggcctgg ctcgatccgg
     1201 acggcgcgca gctctcccgt ccgtggtggt ccctgttggt cgcgaccgcc gggccctcca
     1261 catcccgcgc ccacgatcaa cacagcgatg agaagtgaaa cggtctgccg gtgcatacac
     1321 gtccgacgca cgggggccac cgacggttcc cgagagctca accccccgcg aggtggcggc
     1381 cgatgatcat gcgctggacc tggttggtcc cctcgacgat ctggagggcc ttggcctcgc
     1441 gcatgtagcg ctcgaccggg tactcgcgca tgtacccgta gccgccgtgc acctggaccg
     1501 cgtcggtcgt cacgcgcatc gcgacgtcgg tggcgaacag cttggccacc gagcacgaca
     1561 ccggcgtccc cacgccctcg tcgcgcgccc gggccgcctc ccggtacagc ctgcgcgccg
     1621 cctccacctt cgcgtgcatg tcggccagca tgaacgccag cccctggaac tcgatcacgg
     1681 gcttcccgaa ctgctccctg tccttagcga atgccaccgc agcatcgagg gccgcctggg
     1741 ccacgcccac accgcatgcg gccatcccca gccggccgcc gtccagggct gcgagggcga
     1801 tggtgaaccc ctggccctcc tcccccacca ggttctcgcc gggcacctcg gccccatcga
     1861 acagcagctc ccgcatcggg ctcgcgttcc agcccatctt ctcctcgggc ttcccgaagt
     1921 cgaagcccgg gtaccccttc tccacgacga aggcggagat ccccttcggc ccctctcccc
     1981 cggtccgagc catcacgagg tagtactccg cctctcctcc gtgtgagatg aaccgcttcg
     2041 atcccgtcag ccgccagccg tcgccgtcgc ggtcggcctt cgtggcgagc gaggcggcgt
     2101 cggagccggc ccccgcctcg gtcagcgcgt aggcccccag ccactcaccg gaggtcaggc
     2161 ggggcaggta cctttccttc aagacgtctg agccgtagtc gtgcaccccg aacgtggcca
     2221 ggtggtgcac ggacagcccg accgccagcg acaccgagcc catcgccagc tcctcgagca
     2281 ccgtgagata cgtcaggtag ggctgggccg ctcccccata cgcctcgtcg tacgggatcc
     2341 cccccagccc catctcgccg agctggcgga acggctcccg agggaactcc cccttctcct
     2401 cgaatgcctc gatctgggga cggacccgtt cgttcacgaa ctcgcggacg agcccgacga
     2461 tctcgcgctg atcctccgag agcccgaagt cccggtccat ggcctctcag cgtagcgaac
     2521 acgggcccga gagcccaccc agcgtctatc ctgaggtccc gatgcgcctc tacaacacgc
     2581 tgaccaggga ggtcgaggac gtccagccgc ccggcgacgg gcccgtgcga atctacacgt
     2641 gcgggcccac cgtctatcgg ccggtccaca tcgggaacct ccggaccttc ctcctgtccg
     2701 atctcgtccg acgcgccctc gagctggagg gccacgaggt cctccaggtc gtcaacatca
     2761 ccgacgtggg ccacatggtg gacgattcca gcgaggaggc cctggaccgg atggagctgg
     2821 ccatggagga cgagggcctc ggcccctggg agatcgccga gaagtacacg cgcgtcttcc
     2881 tcgaggacgc cgacaagatc gggctcctgc gcgcccacga gtacccgcgc gccaccgacc
     2941 acgtcgccga gatgatcgag atgacgaaga ccctcatcga cgagggccac gcctacgccc
     3001 tcgacgacgg ctcggtctac tacgacgtcc ggtcgttccc cgactacggg aggctctccc
     3061 ggaacaccct ggacaagctg cagcccggcc acagggatct cgaggccgac gaccgcaagc
     3121 gccaccacgc cgacttcgcc ttgtggaagc gggcccagcc caaccggctg atgaagtggg
     3181 actcgccctg gggcgagggc ttccccggct ggcacatcga gtgctcggcc atgtccctga
     3241 agtacctcgg cgaccgcttc gacatccata cggggggcgt cgacctcgtc ttcccgcacc
     3301 acgaagacga gatcgcccag tccgacgggg cgaccgggca tcgggtggtc tccacctggg
     3361 tccacggcgg ccatctgcgc gtcgagggcc agaagatggc caagtccacg ggcaacgtgt
     3421 acacgatcga cgacgtgatc gagggaggct acgatccgct cgccttccgc ctcctctgct
     3481 tccagacccg ctaccggagc gagatgaact tcacctggga ggcgatggag gcgaccgacc
     3541 gcgcggtcac gagatggcgc cagcgcatgg ccgagtggaa ggacgcgccg agcggcgagc
     3601 cctcccgtga gggagaagtc ttcgaccggc ggttccggga ggcggtggcc gatgacctcg
     3661 acatgccccg cgccgtggcg gtggtgggcg ccacggtctc ctcggagatc ccggagggcg
     3721 agaagcgcgc tctgctctcg tcctgggacg ccgtgctcgg cctcgacctc gaccgcatcg
     3781 tccgcgaggg gttcgaggtc cccgcggacg tccgctcgct ggtcgaggag cgggacgccg
     3841 ctcgggccgg gcgcgactac gcgcgctccg acgagatccg cgaccggctg tccgagatgg
     3901 gctgggaggt catggactcc gcggagggga cgagggtgcg cccgctggcg tagcctgaca
     3961 cgcgatggac gccgcgatcc tgacgcaggg cctgacgaag tactacggca agcgtcccgg
     4021 cgtcctcgag gtggacctcg aggtccgccc cggcgaggtc ttcggtttcc tcggccccaa
     4081 cggggccggc aagaccacca tgatccgcac cctcatggac ctgatccgtc ccacgagcgg
     4141 cagggcgttc gtcctcggga tggacgccca gcgcgaaggg gtcgaggtgc ggcggcgggt
     4201 cggctacctc cccggggacc tcgccctgga cgagcacctc gaggctcgcg acctcctggg
     4261 ctacctctcc tcgcttcggg gcggcgtcca cgaagatgag atcgagcgcc tcgcatcgct
     4321 cctggacgcc gagctcgacg cccggatcgg ctcgctctcc agcggcaacc ggcagaagat
     4381 cgggatcgtc caggcgttca tgcaccggcc ggagctggtc gtgctcgacg agcccacgct
     4441 gggcctcgat cccctgatcc agcagcacgt gtaccggctc ctggccgaga cgaaggccga
     4501 cggccggacg gtgttcctct cctcccacaa ccttcccgag gtcgagcgga cctgcgaccg
     4561 cgtcgggatc atccgtggcg gccggctcgt cgcggtcgag gacgtccggg agatccgctc
     4621 gcgggcgctg cgggagatgg agatccactt cgccgcgccg ccgacgggca acgggttctc
     4681 gcggctcccc ggggtccgcg aggtccacgt ctccggcgaa tccgtgcgga tgcaggtgga
     4741 gggagaggtg gacgcggtgg tgaaggaggc cgcgaagcac cgcgtggtct cgatcacgac
     4801 ccgccagccc acgctcgagg agatcttcct cgcgttctac ggggaggagg aggcctgatg
     4861 gccagcatct tcgtgaaggc gctccgcgat cgacgacgcg tgctgatctg gtgggcgctg
     4921 ggcatgattg ccaccgccct gatgctcgcc tcgttctatc cgacgatccg ggagaccggg
     4981 gaggacctca acgcctacat cgagaacctg ccggaggcgc tccgggcgat cgtgctgtcg
     5041 gggggaggcg acttcacctc gggaccgggc tacctcaaca gcgagctctt ctcgttcatg
     5101 ctcccgctga tcgtgctcgt gttcagcgtg gggatgggag ccggggttgt ggccggagag
     5161 gagcgcgagg gtcgcctcga gctcatggtg gcgagccccg tcacccgccg caggatcgtc
     5221 ctgcagaagc tggcggcgat cctcgtcggg atgatcgggc tggggatcgt cctgtgggcc
     5281 acgctggccg tcggcgtcga gatctgggac atcccggtgg atcacgcgaa ccttgtggcc
     5341 gcgtgtgcca tggcggtgtt cctggggtgg gtgttcgggg cgctcgcgct ggccgtggga
     5401 gcggcaacgg ggcgccgggg catggctatc gggatccccg ccgcggtggg tgtggccacg
     5461 tacctcgtca acggcctggc gcctctgtcc gacgcgctcg agttctggcg gcccctctcg
     5521 cccttctact actacgcggc cgaccagccc atccgcagcg gcatcgacct cggcgacgcc
     5581 ggcatcctcc tcgccctcag cgtcatcctg ggcatcgcgg ccgtgctcgc cttcgaccgc
     5641 cgggatctgg ccacatgagc agccgggaga acggccagcc ccgctggcgc ccggccatcc
     5701 cgaccgtgct ggtcgtggcc tcgatcgtcg tcgcgctcgt ggccgtctcc gcgctgctgt
     5761 acgtgctctt cagggagacg gtggggccgg gggaggtcct gcgcgacttc gccgaggcgc
     5821 tgtccgagga cgactgcccg ggctcctacg acctcctgga cggaagcgtc cgagcatcga
     5881 tcacggagga ggagtggtgc gacgaggtgc ccggcctcgc cgcgaagctg tcaccgcggt
     5941 tcgagatcga gcgcgtcatc ctcagcggag gcgacgcgcg cgttgaggtt tcgggccccg
     6001 agacgacgac cggggcctgg ttcctgcggc ggcagggtcg ctcgtggacc gtgctcgggg
     6061 gcggcgacgc gatcgagttc ccgaccgagc tgccgaccta gaggaccggg agccccaggg
     6121 tcttggccac gttctgccgg aggatctccg acgtcccctc gtacagccgc atggcccgga
     6181 cgtcgcggta ccagacctcg aggccgagct cggtcatgta gccctggccg ccgtggacct
     6241 ggatggccct gtcggcgatc ctgccgaccg cctccgtgga caccagcttg gccagcgctc
     6301 cctccctgcg gccgtcggcc ccgtcgtcga cgtcggccgc cagcttgtag accaggagcc
     6361 tcgcctgctc gagctcggcg taggagtcca cgaccatccc ctgcacgtac tggttggcgc
     6421 cgatcggacg gtcgaaggcg atcctcgatc ccgcgtactc cttcatgcgc tccaggaggt
     6481 gccccgcggt cccgagggcc gtcgcggcga tgccgaaccg ggccccgttg atgcccttta
     6541 tggcggagta gaagccggcg ccgacctccc ccagcacggc cgcatcgggg acctcgcagt
     6601 cctcgaacgt cagctccacg gggttctgga gcggcgacat cgtgcgctgg atccgggtga
     6661 cgctgtagcc cggggtatcg gcgtccacga ggaacgcggt gatgcctcca cgagcaccct
     6721 tctgctggtc ggtgacggcg aagacgaggg cgaagtcggc cgagtccccc gcgctgatga
     6781 agtgcttccg gccgctgagg acccagcctc cgtcggtcct ctcggccctg gtgcggatcc
     6841 gagtggcgtc ggagccggcc tcgggctcgg tgagcgcgaa gcagttgagc ttgtcggcgg
     6901 tcatgagggg cgccaagtac ttctcgcgct gctccggcgt gcagtcgagg tgcacgcggc
     6961 tgggcccgga gacgatgggg aagatcgcct cggcgtgggc caggacgagc ccgctacgaa
     7021 cggcttcctc ggccagcagc acctgcccca ggaacggcag atccatcccg ccgacctcct
     7081 cgggcatgtg gatgccccag aatccgagct ccgcggagcg cttccggagc ttcgcccgct
     7141 cgtccttgat gaaggagtag tcgccctcct ggatctgctg ggcgaaggcc tcttccacgg
     7201 gcgcgatctc gcgttcgacg aaccgccgga gcgtgctgcg gagctcgagg agctcctcgg
     7261 ggatgtccag cagggccacg ggcctcctcc gttcgcctct tgagaaccta ccgaggcgta
     7321 ggttatcacg ggttccgggc ctaccgaacc tccgccagct cccgtggggc gtggttgagg
     7381 cggcgcgcgc cctcctcggt cacgaccacg atatcctcga tcctgacgcc gaggtcccct
     7441 tccaggtaga tcccgggctc gatcgagaag cacatccccg ggcggaggga gtcccggttc
     7501 ccgcgcacga tgtaggggtc ctcgtgggcc tccaggccga tgccgtgccc ggtccggtgg
     7561 atgaagcggt cgccgtaccc ggcgcgctcg atcacctggc gggcggcgcg atccacgtcc
     7621 tcggccggca cgcccgggta gacggcccgg agggcctcct cctgggcctc ggccaccacc
     7681 tcgtggacct cgcggagccg cgccggggcc ttccccaccg ccaccgtccg ggtcatgtcc
     7741 gagcagtagc cgcccgacgt gccgccgaag tccatcacca ccgcgtcccc cgcacgcagg
     7801 gggcggtcca tcgggtcgtg gtgggggctg gccgcgttgg gtccggaacc cacgatggcg
     7861 aactcgacgc gctccagccc cagctcgagc atcagctcgc ccaggcgtcc ggccacctcg
     7921 cgctcggtcg ccccctcgat cctctcctcg aggagccggc ggaaggcctc gtcggtggcc
     7981 cgtgctgctc tggccagcat gtggagctcg tgacggtcct tgacggagcg gagggggccc
     8041 agaacgcgcg aggcggtgac ggcccgggcc ttggggagcg cgcgctggat cccgaggaga
     8101 tgcgaggccc acatgcgatc ggagcacccc agcttcgagg acttggagcc caccacgccc
     8161 gccacgagct cgtacggatc ctcggtctca tcccacgatc taatctcggt gacgtggcgg
     8221 gccagccgcc cttcggcgag ggggcgctcc aggccgggga cgacgagcac ggggtcgtgc
     8281 tcgggccgga tcacgaggca cgtgagccgc tcgagcggcg gcgggtcgta cccggtgagg
     8341 tagatgaggt cggcagaggg cgtgacgagc agggcgtgga ggtcggcctc cgcggtggcc
     8401 tccgccgccc ggcggaggcg ctcggtgtgc tctcgcccca tgaggcccac catcatacgg
     8461 agtgagcggg ggcccggccg ggggttcgat agactccccg gcatggcgtg gaagcagcag
     8521 cggctcggcc ctcggctgct cgaggccgcg cgctccggcg accggtaccg cgacgtccac
     8581 atcgtcctcg gcgggactgg ggccgtcggt gggaccgcgg tgctccagct cctctcgctc
     8641 tacgaagaga tgttctcggt ggcgcctccg ggccccgacg acgtccccgt gctcgtggcc
     8701 accggcgtga ccggcgacga gatcagcgcc ttcacgcggc ggctcttccg gttcgtggag
     8761 tcgcgacacg gggccgaggc gcttcccgag cgggtccggc gaggctatct gacgcactca
     8821 ggggtgttcg tcgcgctcga gcggttcatg gtgacggtgc tgcccgcgct gcgtcgcctt
     8881 cgccaggtgc cgcccgaggt ccgtccgcgc gtgatcgagg agctcctcgc cgagctcggg
     8941 accacgccgg agggcgcggc cgacgcgatt gccgagacgg tctcgggggc ccggcccttc
     9001 acctcgttcc tccaggccta ccgggacgag cacctaggcg ggggccggtt ccgctcggtc
     9061 gtcgtgggga tcccgatccc gagcctgatc gcctaccacc agcaggagct cgacgaggcc
     9121 gccgagcacc tcccggggct gacccccgac cggctcgaga acctgaagca gcggttcatc
     9181 tcggccctgc gcgacgacct cgtggcggtc caggcctccc tggccgacga ggtgctgatc
     9241 gcccacacga cgggggtggg cgggatgtac gatccccgct ccgacggcgg ctccgagatc
     9301 cggctggggt tcgcccacgc cgccctggac ttggcgctgg ccgagaaggc ccgcttcgcc
     9361 caggagctca cagacctcta ttcgcaggcg gggatcaagg tgctggtgac cgcggcggcg
     9421 atcgggatcg acgaggtgaa ggtgcgcgag cgggtcccgc tccaccgtgg ggtccgccac
     9481 cggctgttcg aggctccggt cgagctcttc cccggcgcga aggcctccca gccgcgcgag
     9541 tcgaaggcca gccgcagggc gggccggccc gtcccggcgg cccagcacat gcgcgtcttt
     9601 cctccgatca ccgtgccgtt cgacgatcct cccagcgagc ccgcccggtt cgagccgggc
     9661 gaagagctcg cgccgacgta cacgatccgg agcggcgaga acggcttctt cagcgtggcc
     9721 gacgccgagt cgctctaccg ggtgatgaag gtggcctcgg ccagcgagct gggcctgctc
     9781 ctggccacgg tggggatgct gggcgacgac ccgtcgcgcc cgtggttcga ggggaacgtc
     9841 tgctactacg gcgagaccga caacggccgg caggtcttcg acttcctgtc acagcccacc
     9901 ctgcgcagga cccagctctc tgggctcgag ccgatgtcgc tgcaggacct gggctcggcc
     9961 aagcaccagg gcgagctcca cacgctggcc ctcctgatcc tgctgcaccg cctccggacc
    10021 ctcgacgtgg acgcgatcga tccctacgtg gacctggagc gcttcgaccc ccgggcgttc
    10081 ttcgagcagc ggtcgaggac gctcaccttc gagcagctcg cgctgtggga gccggaggag
    10141 ctggtgcgcg acatgcaggc cctggccgcg gccgagaccc tcgacgatct gctgcccctg
    10201 gcgccacacc gcgagcacga gctgttcccc cggcgagccg aggccgtccg gcgggtcctg
    10261 ggcgaggtcc tgaaggccgt gtggaccggg ccggccctcg gcctgccgat cctctacgag
    10321 cgcgacggcg tctcccatct ccggtgcggc tactacgcgg cgccgctcga cctgctcgta
    10381 accgataccg atgcggtcgc ggtgtggatg cggaaggcgc acgcggagag cggcaacccc
    10441 gcgccgttcg aagacttccg cgactaccac ctcgccgtgg gcggcttcgt ggacgtccgg
    10501 ccgcacgcca tcgtctgcgc cgcgaagagc gaccgcgagg acctcacggg caaggtggcg
    10561 cgcttcgagg acgaggggag gctccgcgag caccttcgga cgctggagcc atactcgttc
    10621 ttctcgacct gcggcttggt cgcggtcctc caccgactca gggcgctgta cgggctgctc
    10681 agggagtcga tgctcgagct cgggaccctg caggaatggc gctggagcat gccgagggac
    10741 gccgcggggc acatggtcgt ccttcccggg gccgccgagg cgctccgcat ggcggccgag
    10801 ggcctggaga agtcgaccgg ggcggagcgc ctcgacggcc cgtggggcta cgagcgacgt
    10861 ccggttcccg accgccgggg cacgatcccc ggcgtccacg accgctagat cttcaggcgg
    10921 agggcttccc tcaggaccgt cacgctgacc ggagtctcac cgagcggcac gccgtcggcc
    10981 tcgaccggga tcgggcggtc ggactcaacg acgatctccg cccccagcat ctccttgatc
    11041 tgcgggtccg ggaggtgctc cccccggtag atgctgggca ggatcgtgaa cgcctgcgac
    11101 ttcgggccga tcgagacctg gacgtcgagt ttcccgtccc ccgggtagga gcggggcgag
    11161 acccgcattc ccccgttcag gaactggccg ttcgcgatca cgacgccgtg ggcgcgcccc
    11221 tcgaactccc gcctgtccgc tcgcacccgc aggttcgaga cccggtagcc gatcaccgaa
    11281 cgccagaagc cgaggaactg acgcccgcgg ccgagccacg ccggcagggc cgcggcgcgc
    11341 cggacggcga ggcctcccag acccacctgc gccgcgttga cgaagtagcg cgagcgcccc
    11401 tcgggcccgt aggtgatccg ggccacgtcg atcgggtacg tgtgctcccc cagcaggtgc
    11461 ctcgccccgg tcgtcacctg gtcgggcagg ccgaaggtgc gcacgaagtc cgcccccgac
    11521 cccgccgcca tcgcccccac cacgaggtcc tcgtcgatcg gcccgtcccc gtccatgagc
    11581 ccgttcacga cctcgttcac ggtcgcgtcg tcgccgacgg cgaccacgaa ccgctccccc
    11641 gactccaccg cctcccgcgc cagccgggag gcgtcgccgg gcttctgcgt ctcctcgatc
    11701 cggtactcga ggcggcccgc cacgatccgc ctctccagct cgggcatctc ccgccccacc
    11761 tcgccacggc ccgcgcgcgg gttgacgatc aggacgaggg ggccgaacgg gctgggcatc
    11821 cctacaggcc cgtgtacgcc acgaccccgc ggttcacggc cgtccgcgcc tcgtggacga
    11881 gcgccgcgag gtcctcgtcc gccacgtcct cgatcatgcg gaggaggtcg atcagctgct
    11941 tgcaggtgcg gacgaagtcg cccggggcca tctcggtctc gcggagcgcg tcctccagcg
    12001 ccttcccctc ggcccagtgg aacgcgggct cggcgaaccc cgcgtcgagc tcgcggcaca
    12061 gctcgacgcc ggcgcgctcc tcggccctcc ggacctcgag gtacagcgac cggagccgcc
    12121 cgaaccgcga ctcgacctcg accgtgggga gcgtgggcga gcggggggtc cgctcccggg
    12181 actcgtagac cacggtggag acgacggcgg cgacctccgc cggggtcagg acgtcgagga
    12241 cgccgtcctc gatcgcctcg gccacgagga tgtctccctc gccgtagatc ctcgtgagcc
    12301 gctccccctt cggggtgagc gagaagtccg agacgtagcc cagcgacgtc aggacctcca
    12361 gcaccctgtc gagctgccgg gcgagcgagt cggtcctggc ccggatccgc cgctccaggc
    12421 cccgcacccg gcggcgcagc ttggaggcgc ggcgcgccca ccgctcgtgc tccttcaggt
    12481 ccgggcacga ggcgaccgga tgcttccgag cccgggcctc gagctcctcg gcgcggctca
    12541 gcgcctcgcg ctggggaccc ccgcggcgcc gcttcggagg ccgcacgttc atggacacga
    12601 gcttcgcggc cacgtctcgg cggtagcggg cgctccgcgc ggaccccctc ttggggagct
    12661 ggacgcgagc cagggactcg ggcgggtcct ggaagtcgcg cggcgtggcc cggaacgcct
    12721 ttcgatcctg ggtgaggacc gagggccgcc cctcgcggtt cgccagcacg acggccaggc
    12781 ctcgcctgcg ggccctgggc acgaagatca cgtcgccggg acggagcctc cctaaagcgt
    12841 ccttgatctc ctcctgccgc cggcgctcct ccgcccggtc ggcctctcgg cggagctcct
    12901 gggcctcctc cagcatcccc cagtactcct cgaagtcccc gcggtcgcag gccagctgct
    12961 ccgcgtaccc cgcgaggccc gcccggtccc gctcggcctg gcgctccagc gcgaccaccg
    13021 cccggtcggc ctggaactgg ccgaacgagg agttcaggag gtggcgggcc tcctccagcg
    13081 tgtagttgcg gacgaggttc accgtcatgt tgtaggaggg gcggaacgag gaggtgagct
    13141 cgtaggtgcg ctggctggcc aggcccgcca cccgctcgaa ctcgacctgc cgctggtgga
    13201 gcaccacggc gtagcccagg tcgtcgatgc cgcgccgacc cgcgcgcccc gtgagctgcg
    13261 tgtactcgcc gggcgtcagc acctcgtggc gctcccccgt gaacttgaag agatcctcga
    13321 tcacgaccgt cttggccggc atgttgatgc cgagcgagag cgtctcggta gcgaagacga
    13381 tcttgaccag cccccgggcg aacagctcct ccaccgtctc cttgaagacc ggcagcatcc
    13441 cggcgtggtg ggcggcgacc cccgcggtca gcgcctcgcg cagctcgtag aaccccaggc
    13501 tctgcaggtc ctcctcgtcc atccacgcgg tccgcatctc cacgaactcc cggatgcgct
    13561 cctgctcctc ggggctcgtc aggcggatcc cggcctccat cgcgtactcg acgctgcggt
    13621 cgcagccggc gcgggagaag acgaagtaga tcgcggggag gatgccctcg cgctccagga
    13681 cccggatcac ttcctcgcgc cgcggcacgt agacgcggcg atgcccttcc cgagggcggc
    13741 cccgccgctc gtagtgcctg cggccgtcgc gtctgcggta ctccttcacc cgcagctcgc
    13801 gctgggagag cgagacgagg taggggttgg gcgcgaggtg gccgtcctcc tcgacgtgca
    13861 tcgggtgcag ctctcgcccg gtcatgtaga ggtgctccag cgggaccggc cggcgctcct
    13921 ccacgatcac tcgggtcgcg ccgcgcaggg tggtgagcca ctcgccgaac tcctcggcgt
    13981 tggagaccgt cgccgacagg gccaccaccg agaccaccag gtcgaggtgg atcaggatct
    14041 cctcccagac cgggccccgg tacggatcct gcagatagtg gacctcgtcc atcacgacga
    14101 cctcgaggtc gcggagggtg gggctctgct ggtacagcat gttccggagg atctcggtgg
    14161 tcatcacgac gatcgaggcc tcggagttga tcgagttgtc gccggtgagg aggccgactt
    14221 cggaggcccc gtacctcgcc acgaagtccc cgtacttctg gttggagagg gccttcagcg
    14281 gggtcgtgta gaaggccttc cggccgcgcg agagggcctg ctcgatcgcg tactcggcca
    14341 cgaccgtctt gcccgagccc gtgggcgccg ccacgagcac cgactcgccg agggccagcg
    14401 cctcgatcgc ccggcgctga tagtcgtcga gcgggaaggg atatcgggag gcgaagcgtt
    14461 cggggtccat cgcccccatc ctcccacgcg atccggtcag gcgcggcgaa ggaggccccg
    14521 cgcggcctcg aggaacccct cgggatcctc caggttcgag aggtgcccgg ctccatcgag
    14581 cacgacgagc tcggcgtccg ggatcgccgc cgccatcgcc tcggacatcg ggggcggcgt
    14641 gagggtgtcc ccggagccga ccacgaccgt ggtggggacg tcgatccccg acaggtccgg
    14701 ccgggagtcg ggtcgctcgg ccatgcccag cgaggcgagg gcgatcgcct ccgccggctg
    14761 gcgggcgatc cacgccttga cctcctccca gagggtgccc gaggcttccg cggacagcag
    14821 aggcggcggg ctctcgacca cgaccccgct cccttcctcc cggacccgct gggccaggtc
    14881 gcggcgcttg gcgcgcccct cctcgtcgtc gggctccgcc cgcgtgtcca cgaggagcag
    14941 gccggctacc cggtcccgat gccgtcgcca catctcgaac gccgcgtagc cccccatcga
    15001 gcacccaccg atgaccgctc ggtcgatccc gacccggtcc atggcggccg cgaccgcgtc
    15061 ggccatcccg gacaccgtca gcgggccgtc cggaaggtcc tctcctccga acccgggcag
    15121 gtggggggcg agcgtccgag cttcgccggc gaggtccgcg acctgccgcc cccacatcgc
    15181 ggcgtccacg gggaaggcat ggatcaggac gaggtcggtc acggggcgat cacctccagg
    15241 gtccggggct ccacggtgag ggtgacgggg agcggaccga agcgctcgcc gtccgcgtac
    15301 acgtcgaagg gtcgcgaggc ctcgagtgca accttgcgag accgcagcat cgtcacgtcg
    15361 cggtgggtga cgtgcgtgcc ccggtagacg ctggggaacg ccgccacgaa gctcctcctc
    15421 gacatcgctc cgaccacgca caggtcgagg aggccgtcat ccatcttggc gtcggggcag
    15481 accatcatcc ctcctccata ggaggcggcg ttggccacgg cgaccatcat ggcgcggagg
    15541 cggaggtcct cgtcgtcggt gaccacccgg aattcggccg gctcgaaggt ccggagcgtc
    15601 cgcatcagag ccacgacgta cttcaccgtc ccggtgagaa ggcgcatgcg gttggccacc
    15661 tcgttcgtct cggagtcgaa gcccgccccc gccacgcaca cgtacgatcg cgtccagccc
    15721 gcgccttcca tgcggacggt gtccaccgtg cgccgagctc cgcggtccag gagcgcgatc
    15781 gagcggaggg ggcgccggtg atccaggccg aggcggcggg cgaagtcgtt gccggcgccg
    15841 gtggggacga cggcgagggg cacgccggtg ccgatcagcg cgttggcgac cgcgctgacg
    15901 agcccgtccc cgcccagggc cacgaccgcc tccgccccgg cgtccagggc ctcgcgggcg
    15961 gcgcgctggg ggtggctccc gtcctcggtg acgacgacgc ggtaggcccg tcccagcgag
    16021 ccgagagccc ggatcgcgcg gggcatgacc cggccgccct tccctccacc ggacgagggg
    16081 ttggcgacca ggacgatcac cgcgtcaccc tgtgggctcg ggggccgggg cctcggggat
    16141 cgggacctct cccggcccgg gcaccccgaa cgggttgttc cccgggagct cggtctggag
    16201 cccgggggtg ccctcgtcca actccacgtc cacccgctcg ttgaggtaga tcaggtagcc
    16261 gctctggacg gtccccgccg ccgtctggag gtcgccctgg agggccacgg tcgcctcggc
    16321 tacccgcgcc tcctgctcct cgtcgaggag cgcggggacg ccctggagga gcacctggaa
    16381 gaactccagg ccgcccgaga tccgctcgat cgatctccgg aggtctcgac ggtcggtctc
    16441 gatcgcccca aggtcgtccc tggtggcggc caggtcctcc tcccagcggg cgatctgacg
    16501 cctcacccgg cgctcccgcg cctccccggc ctggatgtcg agcagggtcg cgcccagctg
    16561 cgggaggacg acgagggact gcccggagac ctgggccacg ggggccacgg cgcgctcgag
    16621 ctcctcgccc acccgctcca cctcgttctg ggcggcctcg cgctcggccg ccgcgcggcg
    16681 cgccgcgatg accccgaacg ccagccccga gacggtcagc acgagcaggc cgatcaggct
    16741 ccagcgcacc caggtgcgct ggaagaacgg cttcttgggg acgacgagcg tgggcctagg
    16801 tgcgacggcc ctcgccctcg accggccccc tctcgagcgc ttctttccct tgatcgccat
    16861 gaaccgacct cagcgcttga agaaccgggc caccaggatg gcggcctcgt agaagagtat
    16921 caacgggacc gtcatgagga gcatcgtgta cgggtcctgc gtgggcgtca cgagcgccgc
    16981 gaagaccacc agcccgaggt acacatagcg gcgccacgtc cgcatctgat cgctcgtgat
    17041 gactcgggcc gccgccagga acatcatcag cagggggaac tcgaacgaca gcccgaacgc
    17101 cagcaccacc atgatgatga accccacgta gcggtccacc gtgagcaggg ggacgaggct
    17161 ctcgcctccg aatccgagca ggaaccgcag cccccgaggg atgatggcgt aggcgaagac
    17221 cgctccgccc gcgaacaggg ccacggagag gaggacgaac ggcgccccga ggcgtcgctc
    17281 ccgcttcgtc agtcccggcg tcacgaaggc ccagacctgg tacaggatca gcggcagcgc
    17341 cagcatgagg ccgctgaaga accccacctt caggaaggtg atgaaggggt cggtggggct
    17401 ggagtagacc agccgcccct ccagggcgcc gcccgggata gcgccgggag gcagggtagc
    17461 cagcgctcgc cggtacgggc gcaggatgat ctcgaggatg tcctggtaga agacgaaccc
    17521 gatcaccgac atcacgagga tcgccacgag cgccacgaag atcctggtgc gcagctcctc
    17581 gaggtgctcg acgaccgaca tcgatccttc gggatcgcgc cgccgcctgc gtgggatcag
    17641 gcgcatggcg aagctcgctt cgggagcgcc gccgggctac tccggcggtc cacccgtctc
    17701 cggctccggc tcctccccgg gggcgcgctc ggaggtctcg ccgttcgcgg tcggcggacg
    17761 cctgcgccgc gccggcgtgc gtcgcggcgg gcccacgtcc aggtcctgct cgaactcggc
    17821 cttgagattg gtggtcgccc gtcggaactc ccggagggac ttccccaccg tgcgaccgat
    17881 ctcgggcagc ctgcgcgggc cgaacacgag cagggccacg agcaggatga cgatgatctc
    17941 tccgaagccg aggttgggca tctcgggcct atcctatccc ggcgccagcg ggtcgtccgg
    18001 gtccacggga accacgcggg ggggccgacc gcgcgagccg gcggcgacgc cgtcccgctg
    18061 cagctccacg aggcgccggc tcgcgcggtc ggccccaccc tggatctcca tgaggatcgg
    18121 ctggagctcc tcctggaact ccacgatcga cgtcaccacc cgcttcatct gcctgacgag
    18181 agagacgacg gcgaccacga gcacgagcag ggtcgcgagc gccacggcga tcacgaacgc
    18241 cagcgggatc acgcgggtca ggatagcagg ggctcagccg gcggatcgcg cgagctcgtc
    18301 gaaccagtct tcctgctgca ggaggaagtg gaagtcgatc agatcgtcca gcgtgaacgc
    18361 aggctcctcc tgggccacgc ggacgtcctc ctgcacggcc tcttcaaagc tcggctggtg
    18421 actgcggacg gaggggtgct ccatgctgac gacctcgtgg gagacctccc gcacctccac
    18481 cccggccgac agcagcagca tgaccacctt gcggtcggca cgcttctcga cggtgtcctc
    18541 gcagctcggg cagacgaacc ggtaggtacc ctcggtggtc ccggcctcga cgacgaggag
    18601 gacggaggcg gcgctcatct ccacctcgcc gcatcgcgga cacgtcgcgc ggatcgtggt
    18661 catctcccca tccgtatcgg caagggggga accgacttta ggcggagcgg gctcaggcgt
    18721 acctggcgag cgcttcctcg gtcatcgtcc gcgccgtctc ccgcagctcg ggggggtgga
    18781 gcacctcgag gtcggccccg acgcgcaaca cgagtttcgc cacccaggcc aggtcggggg
    18841 tggggagggt gacctcgagc ccgccgtcga cctcccgctc gtcgtccacc tcgtagtact
    18901 cggataccca gcgggcccgc gggccgagcc ggagacggac ggccacgtcg cgatcggtcg
    18961 gcgtgtagag aggccgaccc gcaccggcca gccccctggg ctcgaacgga tccccggtgc
    19021 ggcgcgcctc gcggatgcgg tcgatccgga acagccgctc cccgtcgcgg aggtggtccc
    19081 aggcgaccgc gtaccaattg cccagtgcgc tgaagacgtg ttccggatcg atccttcgcg
    19141 tcgtcacctc gtcccggctg gcggagtagt agtcgatctc cagggtctcc cgccgctcca
    19201 cggcccctcg gacggcatcc aggggaccgg agggcttccc ctcccccgcc tcgatctgaa
    19261 ggtcgcccag cgtctcaccg aggtgctcct tgagcttgtc cagggccgag cggagggcgt
    19321 cggcctcgct caggccctgt gcgccgagga agaccgtccc cttcaggtag agagcgaggg
    19381 cctcagccgg cgtcaggcgg accggccgcc gaaagtagtc ggccatcccg atccacaccc
    19441 ggccatcctc gatgtccacc tcgatcaggt tgcccgggtc gtagggcgga agccccgtca
    19501 tgaacagcag gttgaggtcc ctggtgagcg agcgggggtc aacgtcgaag aggcggccca
    19561 gctcctcgag ggtcgtgccg gggtgccgga tcacgtaggg cacgatcgcc agcaggcgct
    19621 cgatgcggcg ggcgacctta gagggaggca cggatctcct ccagggattc ggccagtcgc
    19681 cggcggaggt cctccggctc gaggagctcg gcatccggcc cgaacgcccg gacccagccc
    19741 aagaacgact cgtcggtcga ggcggggacc tcgagcacag tccacccgtc gtcgcgcgtc
    19801 tccaccacgg tcgagccggg agcctggtcc cgggcccacc atgccacctt cggcgcgaag
    19861 gccacggtgg cggtggtctg gggctccccc accccccagg gcccgctacg gagccgatcc
    19921 cgggtgtcga acccctcggg aggcgcgtcc gcttcgccga tatcctcgag gccctccacg
    19981 atccgcgaga gccggaagct ccgcacgtcc tgggccgagc ggtcgcgtcc caccaggtac
    20041 caggctcccc ggaaagacac gagtccccag gcgtcgacct cccggtcgga gacctcgccg
    20101 ccgatcggcc ggtaggcgaa gcggacgagg cgccggcggg ccgccgcgtc cgcggcatcc
    20161 atcacgttca cgctcgagtc gtcgagcccc gggacccggc ggtcgacgag ccccgagatc
    20221 accgccgtgt ccgcgtcgcg ggccagcttc ccgaaggcct gggccgcctg ctcgtagggg
    20281 ccgggagcgt gcgcggcgat gaagagggcc gcgatctctt cgggggtgaa cgagatctcg
    20341 ggcaggtagt accgctcctt ggggatgtgg tagcccacct cgacctcgta gacgtcggtg
    20401 ccgcccgtct cgatggggat cccgatccgg cgaaggtcgt ccttgtcccg ctcgaactgg
    20461 cgcttggcga cctcgcggtc ctcctgcgtg tacgccccca tctcgtcgcg gatctcgtcg
    20521 aacgtgaggg gacggggagc gttgaggagg agggcgacca gcttgaggag gcgctccaag
    20581 ggctccatcg cggcccgata gtacccactc ggggggcgcg gacgacgggg ggcggcccgg
    20641 ggcgtctacg atacgcgggt gatccggctc aggcacggcg tcgtgcgatc ggtggaggct
    20701 caccggcccg gcctccttga gctcggtgtc gaggtggagg gaaggagcga gctcgcgatc
    20761 gcctacccgc agttgaccgg gccggtgcga gagggcgacc gggtgctcct gaacacgacg
    20821 gcggtcgtcg cggggctggg cagcggcggc taccacttcg tgctggccgt cgaggaggcc
    20881 ccggacgccg atccccccgg cgagggccac gtcatgaagc ttcgctacac gccgcttcag
    20941 gcgaaggtgc tcgccgtgga ggagcaggac agcccggatc gcgaggcggt catggatgcc
    21001 gactcgctgg acggcctccc cgtcgtctgg atccctctgc actcgatgct cggcgcggcc
    21061 tgcgcggggg ccagggccgc cggggctcgg cggatcgccc acgtgatgac cgacggcgcg
    21121 gccctgccca tctggctctc gcgccaggtc cacgagctgc gcgaggcggg gctcctcgac
    21181 gtcacgatca cggccgggca ggctctgggg ggcgatcggg aggccgtgaa cgtcttctcg
    21241 gccctcctgg cggcccggga ggtcgaggag gcggacgtcg tcgtcgtggg cgacggcccc
    21301 gggaaggtgg gcaccgcgac ccgatgggga gccaccgagg tctccggggg cctggctctc
    21361 caggccgccg cgatcctggg cgcacgggcc atcgccgccg tccggatcaa cttcgcggat
    21421 cccacctacc gccatcacgg gctctcgccc cactcgctca cggtcctgag ccgcgtggcg
    21481 agccccgggg cgcacgtggc cgtccccact ctcgagggcc cgcggcggga tgtggtctgg
    21541 gaggcgatac gcgaggcgaa gctcgaggag cggcaccaag tggtggaggt cacagggcag
    21601 ccggccctcg atctcctggc ggaacgcggc gtgggcgcgg agacgatggg ccggaagctc
    21661 gaggatgagc cggagttctt cctggccgcc ggcgcggccg gtgtcctggc gggccggatg
    21721 gcggcccgca cggagcgctg gaagtcggag acttgatggc caggacgctc gaggtgacgt
    21781 tcaacgcgac cggggccgcc gccgccggcg cccgcggccg ggtcgtgtgc gtggtggacg
    21841 tcgtggactc gtcgacgagc gccgaggcgg cgctgtcggc cggcgcggcg gccgtcctcg
    21901 gcgccgcgcc ggccgacgcc gacccaccgg tcaccgtgcg gccggacgca gtgggcatgc
    21961 gggcggtggc gctggccggc gagcacgacg cctcggtgct ggtcgtcgcg gaaccgcgcg
    22021 tgggcagcca ggaggagcgt caggagcggg ccgccgccgt cctcaacggg atccagggcg
    22081 cggcgatcga gtggcagctg gttccgaacc agggtgcgga gctgccggcc ctcgcctcct
    22141 gcgcggggag gatcgtcgtg gtcgtgtcca c
//
LOCUS       DASUTZ010000103        13220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1192683, whole genome shotgun sequence.
ACCESSION   DASUTZ010000103 DASUTZ010000000
VERSION     DASUTZ010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13220
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192683"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VF097_04620"
     CDS             complement(<1..749)
                     /locus_tag="VF097_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6262112.1"
                     /translation="MSETRPFLVAGEARTRDETFEVKSPYDGSVVAEIARPTDEDVEE
                     ALAKAADAFEETRALPTHVRAEALMHVSRRIAERADEAAEIIAREGGKPMKWASVEAK
                     RAVSTFRWAAEEIRQGAGELRRLDADAGMEGRLGVMRRFPYGPVLGITPFNFPVNLVA
                     HKLAPAIGVGAPIVIKPATQTPIGALFLAELVAETDLPGGILSVLPISGSKAEPLVTD
                     PRIKKVSFTGSGPVGWRLKGLDPRKRFTLELG"
     gene            complement(766..2211)
                     /locus_tag="VF097_04625"
     CDS             complement(766..2211)
                     /locus_tag="VF097_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HEX6262113.1"
                     /translation="MADHKKMFIGGEWVDGSGDSVSVINPASGAVIAETPNATEEDVD
                     RAVQTAKRTFEETWFDSTPGERQAMMLAWADAIEANAEELGRIESENVGKPVSWVVSD
                     EIMVVAEQMRFFAGAARVMGGLAAGEYYKEDSTLFTSWLRREPLGVVGLIAPWNYPLY
                     MAMWKFAPALAAGNTIVIKPSEWTPLTLLRAAELAADIFPPGVINVVTGEGEPTGASI
                     VRHPDVAMVSLTGEVTTGKTIAREASETLKRVHLELGGKAPVVVFDDADLESSVEWIK
                     TAGYFNSGQDCTAATRVIAGPKIYDALLSNLVSGVEGMKVGDPSEEDTEMGPVVAEQQ
                     LQRVTGFVDRAREQGASVLTGGERIGDKGFFYSPTVVADVGQDFEIVKNEVFGPVVTV
                     QRFEDDEQAVAWANDVDYGLAASVWTRDVGRALNASRKLRFGTVWINTHIPLTPEMPH
                     GGFKQSGYGKDMSVYSLEHYTELKHVMAAID"
     gene            2360..3499
                     /locus_tag="VF097_04630"
     CDS             2360..3499
                     /locus_tag="VF097_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262114.1"
                     /translation="MAVCLRCEWEGPLPAPERCPSCGVPLFEAGMRPATRSRARTAQG
                     GTAVLEAEVVERRSEPEGPPRPSPRRWALPLALVAVAAAIPLLPRQEAPRPARPGVAN
                     PPSQTLRFIGLPPGLSDRTVDRPVRIAFSATQPVPEDRAQLFQLWRLDLPTGELTPGP
                     IVGDVLLMRYAPDDSGRLAFLVSGGGLFVLDGFLASRPTWVDGEVNAFDFAPGGTLVY
                     AKVERRSAPGGGALAAVRLTAVEPGRTTPSTVRSRTIHRLNLRGYAVRGWELIGWGVR
                     DGEEQAIAWNVRDGRVRRAPELRLTPRARSGAGSWELGARGVGRISRSFPRDPAHLAV
                     SGGLAFWAGGDGLTVAERDGGRSFVVELPPRFPIPTGPVAATRGR"
     gene            complement(3544..4452)
                     /locus_tag="VF097_04635"
     CDS             complement(3544..4452)
                     /locus_tag="VF097_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03620 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX6262115.1"
                     /translation="METDGRAAARALGRVGVWTFTLDANPAAAAREAVAELDELGYGA
                     VWIPEGRGSKEVLSHAAVLLGASDRIPLATGIANIWFRDPAAMASGARTLADAFPGRF
                     VLGVGVSHAPTVERRLGLAYERPYSRMVDYLDGMEAARYPAGGAGGAPPLVLAALGPR
                     MLRLAAGRAAGAHPYFVSVEHTAQAREILGEAPLLAPEQAVVLETDPGRAREVARGYM
                     GHYLKLPNYAGNLRRLGWSDDDVDGPSDALVDAIVAWGDEDAVVRRVREHLDAGADHV
                     SVQVLLEDARAIPLDGYRRLAPALLD"
     gene            complement(4463..5290)
                     /locus_tag="VF097_04640"
     CDS             complement(4463..5290)
                     /locus_tag="VF097_04640"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020395056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6262116.1"
                     /translation="MGRGWGLRVASGAVILFLYFPITIIFLYAFTTETAAFTFPPPAL
                     TTRWFGVAADNPAIWEALRLSLTVAAITTAIALVFGSLMAAAVYRTRFFGREAISFLV
                     VLPIALPGIVTGIALRSSIAILDVPFSLWTIVIGHATFTVVIVYNNVLARFRRMSPSL
                     VEASMDLGASGWQTFRHILLPNLGTALLAGGLLAFALSFDEIIVTTFTAGQQQTLPIW
                     IFSSLFRPRARPVTNVVAIMVVLVTAIPILLAARLARGESGGRIGARPRAGPGGGTQ"
     gene            complement(5290..6222)
                     /locus_tag="VF097_04645"
     CDS             complement(5290..6222)
                     /locus_tag="VF097_04645"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011707521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6262117.1"
                     /translation="MAVATETVPIARRVSAFLYRHPGIKLALLLSPGLALLGMVYGGS
                     LAALLLQSLYRLEEFTGLIVQDISLDTLGQLTDRAHLDIIARTTAMAATVTIGCAAVA
                     FPLAYYMAKFASPRMKTFLYLLVLMPLWSNYLVRVISWKLILAQEGIFNWFVDKLALD
                     GVLRWALGAPVIGGPSISQSFLGMWIVFVYTWLPFMILPLSAALERVPDSLTDASGDL
                     GARPAMTFRKVILPLALPGLIAGSIFTFSLTLGDFIIPSVIGNSTFFIGPFVLLQQGT
                     AGNLPLAAAMSLVPLVIMTLYLLGARRAGAFEAL"
     gene            complement(6299..7525)
                     /locus_tag="VF097_04650"
     CDS             complement(6299..7525)
                     /locus_tag="VF097_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6262118.1"
                     /translation="MRAQLRWWALLAVVVLAAASCGDDGGDGEAAGGGGGGMITELGE
                     NEGQVSIIAWAGYIEDGSTDPAYDWVSGYEEETGCEVSVKTAATSDEMVALMQEGSGF
                     DLVTASGDASLRLITGGTVQPINLDLVPSYDTVDERLQDAPWHTVDTDGDGEAEHYGA
                     PYEWGSNVLMYNTEAFAGEAPTSWSAVFEEQTLPDGESNRGRVQAYDGPIYVADAALY
                     LMHHQPELGITDPYALTREQFDAALELLRQQRELVSRYWHDAFIQIDDFTNEGIAASS
                     SWPFQVNILKAEGEPIDSVIPEEGATGWADTTMMHVNAPHPNCAYRWLEWSLNPKVQG
                     DLAAWFGSVPSVPAACEESELLGPDGCETNGFDNFDQIHFWKTPQSDCFEPGGGKECV
                     PYHEWVTSYVAVIGGR"
     gene            complement(7522..8625)
                     /locus_tag="VF097_04655"
     CDS             complement(7522..8625)
                     /locus_tag="VF097_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6262119.1"
                     /translation="MTETTSAVEFRNVRKRFGDVVAVDGVDLEIRQGEFFSLLGPSGS
                     GKTTCLRMIAGFELPSEGTVHLSGRDVTRLPPYDRDVNTVFQDYALFPHMTVADNVGY
                     GLMVRKAPRDERREHVAEALRMVRLEGFDGRKPQELSGGQRQRVALARALVNQPSVLL
                     LDEPLGALDLKLRQQMQIELKAIQERVGLTFIYVTHDQEEALTMSDRMAVLNEGRVEQ
                     VGAPADVYERPTSEFVAGFVGVSNVLHGEAARAITGEAGSFTIRPEKIRIASLDDRPG
                     PDEHSATGSVRDVVYLGAQTRYFVELDSGEGLVVLRQNLHGTSTDALQVQGRKVRLIW
                     NKQFNRSVEGAEGDAGPAGPGAGPAGPGEEEDA"
     gene            complement(8810..10363)
                     /locus_tag="VF097_04660"
     CDS             complement(8810..10363)
                     /locus_tag="VF097_04660"
                     /inference="COORDINATES: protein motif:HMM:NF019520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HEX6262120.1"
                     /translation="MTLRVDDILRSPGLALQLVGGRSGKGSIVRWVHVSELEDPTPWL
                     KGGELLLTTGMGVGRSAAKQRSYLRRLADAELAGLGFGTGFSFTKVPKAIRDAADELG
                     FPVFEVPYAVPFIAITEMVFSRAAGEQVEILQRSIEAQTALTRAVIDGEGVDGVVREL
                     AGVVGGWAVILELHGVALATAPASAARRTARLWEEIQARRADGPGFSLSLVDRGDHVS
                     IQPVGARGHVEAFLAVGKGEALSQLDRVVAAHAVSLVAIELDRRRAVVDTERRLRGDF
                     LDALVRGELSARKAAEGLHRFGFERTDEVAVLALESPFPVEDLLWAAEDVLSRRGGGY
                     LTAPREQDLYVLVHPDGEEGFGELRREVAGKLRSSVRLGAGSAVRPTQAGTSLREASY
                     ALKVARLEGRDSADFRALGTYRLLLSLQEPDALRAFADSVLAPLDRYDADHGGELVPS
                     LRAFLEHNARWESAAGELFVHRHTLRYRMRKVEELAGRDLGSAHDRMEFWLALRARDL
                     LRETERPAS"
     gene            10516..11859
                     /gene="gabT"
                     /locus_tag="VF097_04665"
     CDS             10516..11859
                     /gene="gabT"
                     /locus_tag="VF097_04665"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEX6262121.1"
                     /translation="MEIPQERRVVTEIPGPRSRELLARREAAIPRGVFNTVPTFVRAA
                     SGGIVEDVDGNRLIDFGTGIAVLNVGNTSPPVVEAIREQAGLYTHTCFHVTMNEPYLA
                     LAERLNELTPGDHDKMTMLVNSGAEAVENAVKISRYATGRPAVVTFDHAFHGRTLLAM
                     SLTGKVMPYKQGFGPFAPDVYRFPISYPYRCATGEPYEECGPRCADLVIDGIEKHIGA
                     ENVACVILEPVQGEGGFVVPGSEFVRRLSAWCAESGALYVADEIQTGFGRTGGWFASE
                     RFEIVPDIVVTAKAMGGGLPIGGVTGPRDAMDAIHVGGLGGTFGGNPVAAAASLAVID
                     QIEREGLLERAERVGATMTEALRGFAERFHLIGDVRGLGAMVAMELVGDRRTKQPAKE
                     AAAQVLHECHAQGLIALKAGTYDNVVRLLPPLTIDDGLLEEGLGILEKALAAVSD"
     gene            complement(11873..12727)
                     /locus_tag="VF097_04670"
     CDS             complement(11873..12727)
                     /locus_tag="VF097_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013807425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6262122.1"
                     /translation="MSPKAHVVRTGLRRGWTEFVLSVRSPQDQGFYIFSAAIVLLYLF
                     FNRDSDVAGTSLSHAAVTLPSILGAMVTFSLIIGPAYQLAMDREDGTLLRARALPHGI
                     LGYVSGQVLTHSLALIPSFAVILLPSVVLFEGVMQGGPGGWLTMVWVIGLGLLAAMPI
                     GIIIGSLAPGVQKVGTWGMLPVLVLFGISGIFSPLQSLWGWVQVVAQVFPMYWLGLGM
                     RSAFLPDAAAAIEIGGSWRTLETVAVLGAWAVAGLLLAPVVLRRMARRQSGSQVEAAR
                     HEAVQWVR"
     gene            complement(12724..>13220)
                     /locus_tag="VF097_04675"
     CDS             complement(12724..>13220)
                     /locus_tag="VF097_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017539698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6262123.1"
                     /translation="GGERRRLDVAIGLVGRPELLFLDEPTAGFDPRARKEFHELIHRL
                     SDLEDTTILMTTHDLDEAERVADRILILARGRIVADGTADELARLVTRDAQVRWSRNG
                     ERFTHATPDATRFVRELFARHGDEVSDLEVRRATLEDTYLEMVQRHEAGVEEAAPGFE
                     GLGR"
BASE COUNT         1969 a         4872 c         4524 g         1855 t
ORIGIN      
        1 ccgagctcga gcgtgaaccg cttccgggga tcgagaccct tcagccgcca gcccacgggc
       61 cccgagcccg tgaacgaaac cttcttgatc cggggatcgg tgacgagcgg ctccgccttc
      121 gatcccgaga tcggcagaac cgagaggatc ccgccgggca ggtcggtctc cgccacgagc
      181 tcggcgagga acagggcccc gatcggcgtc tgcgtcgcgg gcttgatcac gatgggcgcc
      241 ccgaccccga tcgcgggggc gagcttgtgc gccacgaggt tcaccgggaa gttgaagggc
      301 gtgatgccca gcacggggcc gtaggggaac cggcgcatca cgccgagccg tccctccatc
      361 ccggcgtcgg cgtcgagccg ccggagctcg cccgccccct ggcggatctc ctcggccgcc
      421 caccggaagg tggacacggc tcgcttcgcc tcgaccgagg cccacttcat cggcttcccg
      481 ccctcccggg cgatgatctc ggcggcctcg tccgcgcgct cggcgatccg cctcgacacg
      541 tgcatcagcg cctcggcccg gacgtgggtg gggagcgcgc gcgtctcctc gaacgcgtcg
      601 gcggccttcg ccagcgcctc ctcgacgtcc tcgtccgtgg gtcgggcgat ctcggccacg
      661 accgagccgt cgtaggggct cttcacctcg aacgtctcgt cccgggtgcg ggcctccccc
      721 gccaccaaga acggcctggt ctcgctcacg gctgggtcac ctcgctcagt cgatcgccgc
      781 catcacgtgc ttcagctccg tgtagtgctc gagggagtag acgctcatgt ccttcccgta
      841 ccccgactgc ttgaagccgc cgtgcggcat ctccggcgtg agggggatgt gcgtattgat
      901 ccacacggtc ccgaagcgga gcttcctcga cgcgttcagg gcccggccca cgtcgcgggt
      961 ccagacggac gcggcgagcc cgtagtccac gtcgttggcc cacgcaaccg cctgctcgtc
     1021 gtcctcgaac cgctggacgg tcaccaccgg accgaagacc tcgttcttga cgatctcgaa
     1081 gtcctgtccc acgtccgcga ccacggtcgg gctgtagaag aaccccttgt ccccgatccg
     1141 ctcgccgccg gtgaggaccg aggcgccctg ctctcgggcg cggtccacga agccggtcac
     1201 gcgctggagc tgctgctcgg ccacgacggg gcccatctcg gtgtcctcct cggagggatc
     1261 gcccaccttc atgccctcga cccccgagac gaggttcgag aggagggcgt cgtagatctt
     1321 cgggccggcg atcacgcggg tggcggccgt gcagtcctgc cccgagttga agtagcccgc
     1381 ggtcttgatc cactccaccg aagactccag gtcggcgtcg tcgaagacca cgaccggcgc
     1441 cttgccgccc agctcgaggt ggacgcgctt cagggtctcg gacgcctcgc gggcgatggt
     1501 cttccccgtc gtgacctccc cggtgagcga gaccatcgcc acatcggggt ggcggacgat
     1561 cgaggcgccc gtcggctcgc cctccccggt gacgacgttg atcacgcccg gagggaagat
     1621 gtccgcggcc agctcggcgg cccgcagcag ggtcaggggc gtccactcgc tcggcttgat
     1681 cacgatcgtg ttgccggcgg ccagggccgg ggcgaacttc cacatcgcca tgtagagggg
     1741 gtagttccac ggagcgatga ggccgacgac gccgaggggc tcgcgccgga gccagctggt
     1801 gaagagggtc gagtcctcct tgtagtactc gcccgccgcc agcccgccca tcacccgagc
     1861 ggcgccggcg aagaagcgca tctgctcggc gacgaccatg atttcgtcgg acaccaccca
     1921 tgagaccggc ttgccgacgt tctcggactc gatccggccg agctcttcgg cgttggcctc
     1981 gatcgcgtcg gcccaggcca gcatcatggc ctggcgctcg cccggggtgg agtcgaacca
     2041 ggtttcctcg aacgtgcgct tcgccgtctg gaccgcgcgg tccacgtcct cctcggtggc
     2101 gttgggggtc tccgcgatga ccgctcccga ggccgggttg atcaccgaga cgctgtcgcc
     2161 gctgccgtcg acccactcgc cgccgatgaa catcttcttg tggtcggcca tgacccctcc
     2221 ttgggaagcc tgcgggttcc cagcctatcg ggagcgcggg gagcgcgtgc tcgacgcaga
     2281 ggccaaggcg accacccgcg atgtacggca cggacaaccc cgtcatcttc ccaggtgggc
     2341 gggggggtat cctcggtccg tggccgtctg tctccgatgc gagtgggagg gccccctccc
     2401 cgcgccggaa cgctgcccct cctgcggcgt cccgctcttc gaggcgggga tgcggccggc
     2461 cacgcgctcc cgcgcgcgta cggcccaggg ggggaccgcc gtcctcgagg ccgaggtcgt
     2521 ggaacggcgg agcgagccgg aaggcccccc tcggccctcg cccaggcgat gggccctccc
     2581 cctggcgctc gttgccgtgg cggcggccat cccattgctg ccccgccagg aggccccacg
     2641 cccggcgcgc ccaggcgtgg ccaacccccc atcccagacc ctccggttca tcgggctgcc
     2701 ccccgggctc tcggaccgca ccgtggaccg acccgtgcgc atcgccttca gcgcgacgca
     2761 gccggtcccc gaggatcggg cgcagctatt ccagctgtgg cggctcgacc tcccgaccgg
     2821 cgagctcaca cccggaccga tcgtgggcga cgtcctgctg atgcgctacg ccccggacga
     2881 ctcggggaga ctcgcgttcc tcgtgagcgg cggggggctg ttcgtgctcg acgggttcct
     2941 cgcctcccgc ccgacctggg tcgacggcga ggtcaacgcg ttcgacttcg cacccggggg
     3001 tacgctcgtc tacgcgaagg tggagcgtcg ctccgcgcct ggcggcggag cgctcgcggc
     3061 cgtgcgcctg accgcggtcg agcccggacg gaccacgccg agcacggttc gaagccggac
     3121 gatccatcgg ctcaacctca gggggtacgc ggtccggggc tgggagctga tcggctgggg
     3181 cgttcgggac ggtgaggagc aggcgatcgc gtggaacgtg cgcgacggac gtgtccgtcg
     3241 cgcccccgag ctccggctca ccccgagggc ccggagcggc gccggctcct gggaattggg
     3301 cgcccgaggt gtcggccgga tctcgcgctc gttcccccgc gaccccgccc atctggcggt
     3361 gtcggggggc ctggcgttct gggccggcgg cgatggactc acggtggcgg agcgggatgg
     3421 aggccggagc ttcgtcgtag agctgccccc gaggttcccc atccccaccg gaccggtcgc
     3481 cgccacccgc ggacggtgag cgccgaccgg ctggtcgatc ggacggcacg gcgcggcgga
     3541 ggactagtcg aggagcgcgg gggcgaggcg acgatacccg tccagcggga tcgcgcgcgc
     3601 gtcctccagg agtacctgca ccgaaacgtg gtcggccccc gcgtcgaggt gctcccggac
     3661 ccgccggacg accgcgtcct cgtcgcccca ggccacgatg gcgtccacga gcgcgtcgga
     3721 ggggccgtcg acgtcgtcgt ccgaccatcc cagccggcgg aggttccccg cgtagttcgg
     3781 cagcttgagg tagtggccca tgtagccgcg ggccacctcg cgggcccggc cgggatcggt
     3841 ctccagcacg accgcctgct ccggcgccag caggggcgcc tcccccagga tctccctggc
     3901 ctgcgccgtg tgctccacgg agacgaagta ggggtgggcg ccggcggcgc gccccgcggc
     3961 caggcgcagc atgcggggac cgagcgccgc gagcacgagc ggaggcgcgc cgccggcgcc
     4021 ccccgccggg taccgcgcgg cctccatccc atccaggtag tcgaccatcc gggagtacgg
     4081 ccgctcgtag gccaacccca gccgccgctc caccgtcggc gcgtggctca ccccgacccc
     4141 gagcacgaag cggccgggga acgcgtcggc cagcgtccgc gcgccgctcg ccatggccgc
     4201 cgggtcccgg aaccagatgt tcgcgatccc cgtggcgagc gggatgcggt cggaggcgcc
     4261 caggaggacc gcggcgtggc tcaggacctc cttgctcccc cggccctcgg ggatccagac
     4321 cgcgccgtac ccgagctcgt ccagctcggc cacggcctcc cgggccgccg cggcggggtt
     4381 cgcgtcgagc gtgaaggtcc agacgcccac acgtcccagc gcacgcgcgg cggccctgcc
     4441 gtccgtctcc atcccgacca gcctactgcg tgccgccgcc gggcccggcc ctcggccggg
     4501 cgccgatccg gccgcccgac tccccccgcg caagccgtgc ggccagcagg atcggtatcg
     4561 ccgtcacaag cacgaccatg atcgccacca cgttcgtcac cggacgggcc cggggacgaa
     4621 acaggctgga gaagatccag atcggcagcg tctgctgctg ccctgcggtg aacgtcgtca
     4681 cgatgatctc gtcgaaggag agcgcgaagg cgagaaggcc ccccgccagc agcgcggtgc
     4741 cgaggttcgg cagcaggatg tgccggaacg tctgccaccc cgacgccccc aggtccatcg
     4801 aggcctcgac gaggctgggc gacatccttc ggaaccgcgc cagcacgttg ttgtagacga
     4861 tgaccaccgt gaaggtggcg tggccgatca cgatcgtcca gaggctgaac ggcacgtcga
     4921 ggatggcgat cgacgagcgg agggcgatgc ccgtcacgat cccgggcagc gcgatcggca
     4981 gcaccacgag gaacgagatc gcctcgcgcc cgaagaagcg cgtccggtag acggccgccg
     5041 ccatgaggct cccgaagacg agcgcgatcg ccgtcgtgat cgccgccacc gtcagcgaca
     5101 gacgaagcgc ctcccagatc gccggattgt cggcggccac gccgaaccac ctcgtcgtca
     5161 gagccggtgg ggggaacgtg aacgcggccg tctcggtcgt gaaggcgtac aggaagatga
     5221 tggtgatcgg gaagtagagg aagaggatca ccgcccccga cgcgacccga agcccccagc
     5281 ccctgcccat cacagcgcct cgaaggcccc ggcccgcctc gcgccgagga ggtagagggt
     5341 catgatcacg agcggcacga gcgacatcgc cgccgccagc gggaggttgc cggccgtccc
     5401 ctgctgcagc agcacgaacg gcccgatgaa gaacgtcgag ttcccgatca cgctcgggat
     5461 gatgaagtcg ccgagcgtga gggagaacgt gaagatcgac cccgcgatga gccccggcag
     5521 ggccaggggg aggatcacct tgcggaacgt catcgcggga cgcgccccga gatcccccga
     5581 cgcgtcggtc agcgagtcgg gaactcgctc cagggcggcg ctgagcggca ggatcatgaa
     5641 cggcagccac gtgtagacga agacgatcca catcccgagg aaggactgcg agatcgaggg
     5701 cccgccgatc accggggccc cgagcgccca tcgcagcacc ccgtccaggg ccagcttgtc
     5761 cacgaaccag ttgaagatgc cctcctgggc caggatcagc ttccacgaga tcacgcggac
     5821 caggtagttg gaccacagcg gcatcaggac gagcaggtac aagaacgtct tcatccgcgg
     5881 cgaggcgaac ttcgccatgt agtaggcgag cggaaacgca accgccgcgc acccgatcgt
     5941 gaccgtggcg gccatggccg tggtccgggc gatgatgtcg aggtgagccc ggtcggtcag
     6001 ctggcccagc gtgtccaggc tgatgtcctg gacgatgagg ccggtgaact cctccagccg
     6061 gtagaggctc tggaggagga gggcagccag cgagcccccg tagaccatcc cgagcagggc
     6121 gagcccgggc gagagcagca gcgcgagctt gatgcccggg tgccggtaca ggaacgccga
     6181 cacgcgccgg gcgatcggga ccgtctcggt cgctaccgcc atgatcctcc gctcgagggc
     6241 ggggtgagcg agggacgggg ccccgggcgg agccccgccc cccgatcgtc gccgcggatc
     6301 agcgcccgcc gatcaccgcg acgtagctcg tgacccattc gtggtacggc acgcactcct
     6361 tcccgccccc aggctcgaag cagtcggact gcggggtctt ccagaagtgg atctggtcga
     6421 agttgtcgaa tccgttcgtc tcgcacccgt ctgggccgag aagctcgctc tcctcgcagg
     6481 ccgcggggac cgacggcacc gagccgaacc aggcggccag gtcgccctgc accttcgggt
     6541 tcagcgacca ctcgagccag cggtacgcgc agttcgggtg cggggcgttc acgtgcatca
     6601 tcgtcgtgtc ggcccagccg gtcgccccct cctcggggat gacgctgtcg atcggctcgc
     6661 cctcggcctt caggatgttc acctggaagg gccaggagga gctcgccgcg atcccctcgt
     6721 tcgtgaagtc gtcgatctgg atgaaggcgt cgtgccagta tcgcgagacg agctcccgct
     6781 gctgccgcag cagctcgagc gcggcgtcga actgctccct cgtcagcgcg tacgggtccg
     6841 tgatgccgag ctcgggctgg tggtgcatca ggtacagcgc ggcgtcggcc acgtagatcg
     6901 gaccgtcgta cgcctgcacc cggcccctgt tcgactcccc gtcggggagc gtctgctcct
     6961 cgaacaccgc gctccagctc gtgggggcct cgcctgcgaa cgcctcggtg ttgtacatca
     7021 ggacgttgga gccccactcg tagggcgcgc cgtagtgctc cgcctcaccg tcgccgtccg
     7081 tgtccacggt gtgccaaggg gcgtcctgga gccgctcgtc gaccgtgtcg tagctcggga
     7141 ccaggtcgag gttgatcggc tggacggtgc ccccggtgat gagccgcagg ctcgcgtccc
     7201 cggacgccgt cacgaggtcg aagcccgaac cctcctgcat cagcgccacc atctcgtcgg
     7261 atgtcgcggc cgtcttgacg ctcacctcgc agccggtctc ctcctcgtag ccgctcaccc
     7321 agtcgtaggc cggatccgtg ctgccgtcct cgatgtagcc ggcccaggcg atgatgctga
     7381 cctggccctc gttctccccg agctcggtga tcatccctcc cccgccgccg cccgcggcct
     7441 cgccgtcgcc gccgtcgtcg ccacagctcg cggccgcgag cacgaccaca gccagcagcg
     7501 cccaccatcg gagttgtgcc ctcatgcgtc ctcctcctct cccgggccgg cgggcccggc
     7561 tcccgggccc gccggcccgg cgtccccctc ggccccctcg accgagcgat tgaactgctt
     7621 gttccagatg agccgcacct tcctgccctg gacctgcagg gcgtcggtcg acgtgccgtg
     7681 gaggttctgc cgcaggacga cgagcccctc gccggagtcg agctccacga agtatcgggt
     7741 ctgcgccccg aggtagacga cgtcgcgcac actgcccgtg gccgagtgct cgtcgggacc
     7801 cggccggtcg tcaaggctcg cgatccggat cttctcgggc cggatcgtga acgacccggc
     7861 ctccccggtg atggcccggg ccgcctcgcc gtggaggacg ttcgagacgc ccacgaaccc
     7921 cgccacgaac tcgctcgtcg gccgctcgta gacgtccgcg ggcgcgccca cctgctccac
     7981 ccgcccctcg ttgaggaccg ccatccggtc gctcatcgtg agcgcctctt cctggtcgtg
     8041 cgtgacgtag atgaacgtga ggcccacgcg ttcctggatc gccttcagct cgatctgcat
     8101 ctgctgacgg agcttcaaat ccagcgcccc gagcggctcg tcgagcagca ggaccgaggg
     8161 ctggttgacg agcgcgcggg ccagagccac ccgctgccgc tggccccccg agagctcctg
     8221 cggcttccgg ccgtcgaacc cctcgagccg caccatccga agggcctccg ccacgtgctc
     8281 ccggcgctcg tcccgcggcg ccttccggac catgaggccg tagcccacgt tgtcggccac
     8341 ggtcatgtga gggaagaggg cgtagtcctg gaagacggtg ttgacgtccc ggtcgtaggg
     8401 agggagacgc gtgacgtccc gccccgagag gtgcaccgtg ccctcgctcg gcagctcgaa
     8461 cccggcgatc atccggagac aggtcgtctt gccggagccc gacggtccga gcagcgagaa
     8521 gaactccccc tggcggatct cgaggtcgac gccgtccacg gcgaccacgt cgccgaagcg
     8581 cttcctgacg ttgcggaact cgacggcgga ggtcgtctcc gtcacctgct cagcgacgtc
     8641 cgtccgtcca tcggtcgaac gtccggctac gttgccgccg gccaaggacc tccctcctcc
     8701 gcaccggacg gttcatacca gagggaggac cggcgaggac gcgcgcgagg gagcaaccac
     8761 cgccccccgc ttgtacggat gggccaagtc tcgcgccgga cgggcgcgat cagctcgcgg
     8821 gccgctcggt ctccctgagg aggtcccggg cccggagcgc cagccagaac tccatccggt
     8881 cgtgagccga ccccagatct ctcccggcca gctcctccac cttccgcatc cgatatcgga
     8941 gggtgtggcg gtgtacgaag agctcccccg ccgccgactc ccacctcgcg ttgtgctcca
     9001 ggaaggcgcg gagcgaggga acgagctcgc ctccgtgatc ggcgtcgtaa cggtccagcg
     9061 gggcgagcac cgagtcggcg aacgcccgca gcgcgtcggg ctcctgcagg ctgaggagca
     9121 ggcggtaggt cccgagggcg cggaagtccg cgctgtcccg accctccagc cgggcgacct
     9181 tcagcgcgta gctcgcctcc cggagactgg ttcccgcttg ggtcgggcgc actgcgctcc
     9241 cggcacccag ccggaccgag ctgcggagct tcccggccac ctcgcgccgg agctcgccga
     9301 agccctcctc gccgtcgggg tggacgagca cgtagaggtc ctgctcgcgc ggcgcggtca
     9361 ggtacccacc gccgcggcgc gagagcacgt cctccgccgc ccacagcaga tcctccaccg
     9421 gaaacgggga ctccagcgcg agcacggcca cctcgtcggt ccgctcgaac ccgaaccggt
     9481 ggagcccctc cgcggccttc cgcgccgaga gctccccgcg caccagcgcg tcgaggaagt
     9541 cccctcggag ccgccgctcc gtgtcgacga ccgcccgtct ccggtcgagc tcgatggcca
     9601 ccagcgacac ggcgtgggcg gccacgaccc gatcgagctg ggacagcgcc tcgcccttcc
     9661 caaccgcgag gaaggcctcc acgtgccccc gggccccgac cggctggatc gagacgtggt
     9721 ctccacggtc cacgagcgag aggctgaagc ccgggccgtc ggcgcgacgg gcctggatct
     9781 cctcccagag gcgcgccgtc cggcgggcgg cggacgcggg cgccgtcgcc agcgccacgc
     9841 cgtggagctc cagaatcact gcccatcccc ccaccacgcc ggcgagctcg cggaccaccc
     9901 catccacccc ttcgccgtcg atgaccgcgc gggtgagggc cgtctgcgcc tcgatcgagc
     9961 gctggaggat ctccacctgc tccccggccg cccgcgagaa gaccatctcc gtgatcgcga
    10021 tgaacgggac ggcgtagggc acctcgaaga cggggaagcc cagctcgtcc gccgcgtcgc
    10081 ggatcgcctt ggggaccttc gtgaacgaga acccggtgcc gaagccgagc ccggccagct
    10141 cggcgtcggc cagcctccgg aggtatgagc gctgcttggc ggcggagcgc cccaccccca
    10201 tgccggtggt cagcaggagc tcgccgccct tcaaccaggg cgtcgggtcc tcgagctcgc
    10261 tcacgtggac ccaccgcacg atgctgccct tccccgagcg tccgcccacc agctgcaggg
    10321 cgagcccggg ggatctgagg atgtcgtcga cgcggagggt cacgtgggga ttgtacggtc
    10381 tgtacaagag caccgtcggg ccggcgggtc gtgtacggtc cgtacaaagg cgggttggtc
    10441 gcttgctcgc cgcggccgtt ccacggccgc ggcgagcaag cgacactagc gccgtccgac
    10501 ccccaggagc tgtccatgga gatcccgcag gagcgccgcg tcgtcaccga gatccccggc
    10561 ccccgctcga gggagctgct ggcccgccgg gaggccgcca tcccccgagg ggtcttcaac
    10621 accgtcccga ccttcgtccg cgcagcgtcg ggcgggatcg tggaggacgt cgacggcaac
    10681 cggctgatcg acttcgggac gggcatcgcc gtgctcaacg tggggaacac ctcacccccc
    10741 gtcgtggagg cgattcgcga gcaggccggc ctctacaccc acacgtgctt ccacgtgacg
    10801 atgaacgagc cctacctcgc cctggccgag cggctcaacg agctgacgcc cggcgaccac
    10861 gacaagatga cgatgctcgt gaactcgggg gcggaggccg ttgagaacgc ggtcaagatc
    10921 tcccgctacg cgaccggccg gcccgccgtc gtcaccttcg accacgcgtt ccacggccgc
    10981 accctcctgg cgatgtcgct tacggggaag gtgatgccct acaagcaggg gttcggcccc
    11041 ttcgcgccgg acgtctaccg cttcccgatc tcctacccgt accgctgcgc caccggtgag
    11101 ccgtacgagg agtgcggacc gcgctgcgcc gacctcgtga tcgacggcat cgagaagcac
    11161 atcggagccg agaacgtggc ctgcgtgatc ctggagcccg tccagggcga gggcggattc
    11221 gtcgtccccg gctccgagtt cgtgcggcgc ctcagcgcct ggtgcgcgga gagcggggcg
    11281 ctctacgtgg cggacgagat ccagaccggc ttcggacgca cgggcgggtg gttcgccagc
    11341 gagcgtttcg aaatcgtccc ggacatcgtc gtgaccgcca aggcgatggg cggcggcctg
    11401 ccgatcggtg gggtgacggg ccccagggac gcgatggacg cgatccacgt tggcgggctc
    11461 gggggcacgt tcggtggcaa ccccgtggcg gccgccgcct cgctcgccgt catcgaccag
    11521 atcgagcgcg aggggctgct ggagcgtgcc gagcgagtcg gcgccacgat gacggaggcc
    11581 ctccgcgggt tcgcggagcg cttccatctg atcggcgacg tgcgcggact cggcgccatg
    11641 gtcgcgatgg agctcgtggg cgaccgcaga acgaagcagc ccgcgaagga ggccgccgcg
    11701 caggtccttc acgagtgcca cgcgcagggc ctgatcgcgc tgaaggccgg gacctacgac
    11761 aacgtcgtgc gcctgctgcc tcccctcacg atcgacgacg ggctcctgga agagggcctg
    11821 ggcatcctgg agaaagcact cgcggcggtg agcgactagg agcccggacg cctcagcgga
    11881 cccactggac cgcctcgtgc cgggcggcct cgacctggga gccggactgc ctgcgggcca
    11941 tcctgcgcag cacgaccggc gccaggagca gtcccgccac cgcccacgca ccgagcaccg
    12001 cgaccgtctc cagcgtccgc cacgagccac cgatctcgat ggccgcggca gcgtccggga
    12061 ggaaagccga tcgcatcccc aggcccagcc agtacatggg gaagacctgc gccacgacct
    12121 ggacccatcc ccacaggctc tggagcgggg agaagatccc tgagatcccg aacagcacga
    12181 ggaccgggag catcccccag gtgccgacct tctgcacccc tggggcaagc gacccgatga
    12241 tgatcccgat cggcatcgcg gccagcagcc cgagcccgat cacccagacc atggtcagcc
    12301 atccacccgg gcccccctgc atcaccccct cgaagagcac gacgctcggg aggaggatca
    12361 ccgcgaacga ggggatgagg gcgagcgagt gcgtcaggac ctgaccggag acgtatccga
    12421 ggatcccatg cggcagggcc ctggcccgaa gcagcgttcc gtcctcgcgg tccatcgcca
    12481 gctggtacgc gggcccgatg atgaggctga acgtgaccat cgccccgagg atgctgggca
    12541 gcgtcacggc cgcatgcgac agggaggtcc ccgccacgtc ggagtccctg ttgaagaaga
    12601 gatagagcag cacgatggcg gccgagaaga tgtagaagcc ctgatcctgc gggctccgaa
    12661 cgctgaggac gaactccgtc caccctcggc ggagccccgt gcgaaccacg tgcgccttgg
    12721 gactcatcgg ccgagcccct cgaagcctgg cgcggcctcc tcgacgccgg cctcgtgacg
    12781 ctgcaccatt tccaggtagg tgtcctccaa cgtcgcccga cgcacctcga gatcggatac
    12841 ctcgtcgccg tgccgggcga acagctcgcg gacgaagcgc gtcgcatccg gcgtcgcgtg
    12901 cgtgaatcgc tcgccgttcc ggctccaccg gacctgcgcg tcacgggtca cgaggcgagc
    12961 gagctcgtcg gcggtcccgt cggccacgat ccgtccccgg gccaggatga ggatgcggtc
    13021 ggccaccctc tccgcctcat ccaggtcgtg ggtggtcatc aagatcgtcg tgtcctcgag
    13081 atccgagagc ctgtggatca gctcgtggaa ctccttgcgc gctcgcgggt cgaagccggc
    13141 cgtgggctcg tcgaggaaga ggagctcggg tcgtccgacc agcccgatcg ccacgtcgag
    13201 ccggcgccgc tcgcccccgg
//
LOCUS       DASUTZ010000104         9545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_286242, whole genome shotgun sequence.
ACCESSION   DASUTZ010000104 DASUTZ010000000
VERSION     DASUTZ010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9545
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286242"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VF097_04680"
     CDS             complement(<1..621)
                     /locus_tag="VF097_04680"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX6262124.1"
                     /translation="MIVLWVVLGIVALVVIGGIVSYNRFVRQRQAIKDAFANIDTELR
                     RRYDLIPNLVETVRGYQIHEKEVLEEVTRARAAATAATGSPAEQAAAEGPLVAALRQL
                     FAVVENYPQLRANENFLALQQELAVTEDRLQTARRFYNANVRDYNQRVQGFPSNIIAS
                     WFNFEEEEFFEIEEAMRETMEQAPQVDFTGTRGEAEPPAASPPPGGE"
     gene            complement(618..1325)
                     /locus_tag="VF097_04685"
     CDS             complement(618..1325)
                     /locus_tag="VF097_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262125.1"
                     /translation="MDPVLIFILGVVGVAVVGGLSWYFAKKRREAFALFARSHGMTYS
                     RHDPYGTVDLPFRLFTMGQGRKVENVLSGEWRGSPVRAFDYWYYTESTDSKGNRTKTY
                     YRHTCALLEIEAAFPPLAITRENVFTRMADGLGFRDIEFESPEFNKRFQVKAKDRHFA
                     YAFIDGGMMKWLLGLPRKWQFEVVGAWILAYERKRMKPEEFPPLIEMALAFRQQVPRA
                     ALSLYGQGVETSREERA"
     gene            1769..2215
                     /locus_tag="VF097_04690"
     CDS             1769..2215
                     /locus_tag="VF097_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262126.1"
                     /translation="MHERDESGARALRDFSVDGQERLQGEGFVDGYATVFGPTGGVTT
                     HLEVTSGAMVFEDSSRAEAGMAVVLEDVEGRLTEATEISADGLGAESAGLSGPVDGDA
                     RQRSALLYVWKTDNLVLFVIASALLESAPSPDVILGLAETMDSRAS"
     gene            complement(2384..2746)
                     /locus_tag="VF097_04695"
     CDS             complement(2384..2746)
                     /locus_tag="VF097_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262127.1"
                     /translation="MHEIGLMYRWGKLVPGRERAGIEHFASAIEFFGKKLTEGVITFF
                     EPYFSRTTDLETEAGFILIKGPREKVLELMEDEEYLVLLTKTSYLVEHFTVEMLTVGE
                     EIQVQLERAQKVFAELGI"
     gene            complement(3064..3711)
                     /locus_tag="VF097_04700"
     CDS             complement(3064..3711)
                     /locus_tag="VF097_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX6262128.1"
                     /translation="MRAPSSRRPVRRRRLDAVWIVAGLGTLLLTGAVASGGTVPGWEQ
                     AVFHAINGLPSWLRVPMQAAEILGVLLVGPAVAVIALGFRRLRLAVAAVLATVLKLAL
                     ERVVKLVVERPRPAVSTPDAIARGVPIRGLAFVSGHAVLTAALAGVVTPYLRGAWRAL
                     PWALVGLVCFARVYLGAHNPLDVVGGAGLGLAIAGALNLILGVPAAGIEEEANPP"
     gene            3859..4470
                     /locus_tag="VF097_04705"
     CDS             3859..4470
                     /locus_tag="VF097_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262129.1"
                     /translation="MAVLTARSATIAVAIALLLGGCVQSDRGSPSLELHRVQPSTREI
                     TPAFLFFMGQFKNDRSPFATPADLDAAADAVVVGTIVAVRPGQSSAPAVESDPVVATS
                     VLEVKVESIVRQDLEVARAGSVYVEVPHPAYVGPGEAGPTGEGTDERHPFDHAAFAAT
                     VPRAYGVFFSTIEHRSHTGTRSWRRALADQPAPGSRPRTFKVS"
     gene            complement(4641..7226)
                     /gene="clpB"
                     /locus_tag="VF097_04710"
     CDS             complement(4641..7226)
                     /gene="clpB"
                     /locus_tag="VF097_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEX6262130.1"
                     /translation="MAFDPNRLTVRSRESLGTAQREARDRNHQAVEPEHVLVALLADP
                     EGVVYPLLHRVGLSPRAVAGRVEERLDQIPKVYGTTQDARLAPATARVLEAAADEAEA
                     LTDEYVSTEHLLLAMLGDQGPLGELLRELGLDRDRLLAALAEVRGRQRVTDENPEEKY
                     EALERYGRDLTDLARRGKLDPVIGRDEEVRRVIQVLSRRTKNNPVLIGEPGVGKTAIA
                     EGLAQRIVAGDVPESLKGRRIVALDIGAMVAGAKYRGEFEDRLKAVLQEIAASEGQII
                     TFIDELHTIVGAGAAEGAVDAGNMLKPLLARGELRMIGATTLDEYRKHVEKDAALERR
                     FQPVFVGEPSVEDTIAILRGLRERYEVHHGVEIKDEALVAAAMLSDRYVTGRFLPDKA
                     IDLVDEAASRLRIEIDSMPVEIDEVDRRVKQLEIERAALKKETDEASKQRLEKIEQEL
                     ADLNEQLSAMRAHWDREKEHISRIHELKSELEETRGQAERAERDGDLERAAELRYGRI
                     VELEKELEAENATLAEVQADRKMLKERVDEEDVAEVVSAWTGVPVSRLLEGEMQKLVR
                     MEEALHERVVGQDEAVTAVANAIRRSRAGLSDPNRPIGSFIFLGPTGVGKTELARALA
                     EFLFDDERAMVRIDMSEYQERHAVARLIGAPPGYVGYEEGGQLTEAVRRRPYSVILLD
                     EIEKAHADAFNVLLQVLDDGRLTDGQGRTVDFKNAVVIMTSNLGSRWFFEETLSPEQR
                     REQVLAEMREAFRPEFLNRVDDVIVFDPLDKEQIAEIVDIQVRRFAERLAERRLTLKL
                     TDDARSYLAERGWDPALGARPLKRLIQKEIQDKLALALLEGEITDGDHLVVDRSGDGL
                     VVQKA"
     gene            complement(7228..7614)
                     /locus_tag="VF097_04715"
     CDS             complement(7228..7614)
                     /locus_tag="VF097_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009305419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX6262131.1"
                     /translation="MAERRDRKREQAVYIISVAAELAGVHPQTLRIYERKGLVRPSRT
                     AGNTRRYSDRDIARLRTIQEMTQAGVNLAGVKRILAMQAEIDELQDELEETKQAIEGL
                     RRRLREERRRNNELVLYESTVLPWER"
     gene            complement(7632..8759)
                     /gene="dnaJ"
                     /locus_tag="VF097_04720"
     CDS             complement(7632..8759)
                     /gene="dnaJ"
                     /locus_tag="VF097_04720"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX6262132.1"
                     /translation="MAASREWLEKDYYAALGVSKSATQAEIKKAYRRLAQKHHPDANP
                     GDKEAEERFKEVSAAYDVLGDEKRRAEYDRVREMASAGFGGGFGGGPGGAGGARVRFE
                     DLGGFGDLFGDLFGGGFGAGAAGPRRGPQRGVDLETEVRISFDQAMEGATVPVRVAGP
                     APCEMCGGSGAKPGTSVRTCTQCGGSGTVAIDQGMFSIAQPCPRCGGSGREIEEPCPT
                     CRGSGTQRRTRTLRVRIPAGVDDGARIRLAGRGEPGGPGGRPGDLYVVVRVSPHERFG
                     RKGSNLTVEIPVTYPEAALGAKVEVPTLNGPVTLKVPAGTPSGKTFRIRGRGIPKRRG
                     GKGDLLATVKVAVPSKVSKKERELLEQLREVSGEGPRPRAS"
     gene            complement(8763..9290)
                     /locus_tag="VF097_04725"
     CDS             complement(8763..9290)
                     /locus_tag="VF097_04725"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX6262133.1"
                     /translation="MTDEQRPDDVVEEVVEGEVEEAPDTSELDEAREQAAEYLNHLQR
                     LKAEFENYRKRVLKEQTRAIELASEDLVLKLLSVVDDFELALVAAEQRPDFEKFLQGV
                     EMVYAKLVDVLRSEGLERIEAKGKLFDPELHEALFQGGEEDGDPYVADVLRTGYTLKG
                     KVVRPAGVKVERREV"
     gene            complement(9287..>9545)
                     /gene="dnaK"
                     /locus_tag="VF097_04730"
     CDS             complement(9287..>9545)
                     /gene="dnaK"
                     /locus_tag="VF097_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="heat shock protein 70; assists in folding of
                     nascent polypeptide chains; refolding of misfolded
                     proteins; utilizes ATPase activity to help fold;
                     co-chaperones are DnaJ and GrpE; multiple copies in some
                     bacteria; Derived by automated computational analysis
                     using gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX6262134.1"
                     /translation="DKVPDDDRQAVETAVADVRKALEGDDLDEIKAKTEALTEAFQKV
                     GQAVYQQEQAAAGGAEGAEAQAPEGEEDVVEGEVVDEGGSS"
BASE COUNT         1380 a         3386 c         3224 g         1555 t
ORIGIN      
        1 ctccccaccc ggaggaggag acgcagccgg aggctccgcc tccccccgcg tccccgtgaa
       61 gtcgacctgc ggggcctgct ccatcgtctc ccgcatcgcc tcctcgatct cgaagaactc
      121 ctcctcctcg aagttgaacc aggaggcgat gatgttcgat gggaagccct gcacccgctg
      181 gttgtagtcg cggacgttcg cgttgtagaa gcggcgtgct gtctggagac ggtcctcggt
      241 gacggcaagc tcctgctgga gggcgaggaa gttctcgttg gcgcggagct gcgggtagtt
      301 ctcgacgacg gcaaacagct gccgcagggc ggcgacgagc ggcccctcgg cggcggcctg
      361 ctcggccggc gaccccgtgg ccgctgtcgc ggcggcgcgg gcccgggtga cctcctcgag
      421 gacctccttc tcgtggatct ggtagccgcg caccgtctcg acgaggttcg ggatgaggtc
      481 gtagcggcgc cgcagctcgg tgtcgatgtt ggcgaacgcg tccttgatcg cctgccgctg
      541 ccggacgaac cggttgtagg acacgatgcc gccgatgacc accagggcga cgatgcccag
      601 gaccacccac aggacgatca tgcgcgctcc tccctcgacg tttcgacgcc ctgaccatac
      661 aggctcagcg cggcccgcgg cacctgctgg cggaaggcga gggccatctc gatcagcggg
      721 gggaactcct cgggcttcat ccgcttcctc tcgtaggcga ggatccaggc ccccacgacc
      781 tcgaactgcc acttgcgggg gaggccgagc agccatttca tcatccctcc gtcgatgaac
      841 gcgtaagcga agtgccggtc cttggccttg acctggaagc gcttgttgaa ctcaggcgac
      901 tcgaactcga tgtcgcggaa cccgagcccg tcggccatcc gcgtgaagac gttctcgcgg
      961 gtgatcgcga gcggagggaa cgcggcctcg atctcgagca gcgcgcacgt gtggcggtag
     1021 taggttttcg tgcggttccc cttgctgtcg gtcgactccg tgtagtacca gtagtcgaag
     1081 gcccggacgg ggctgcctcg ccactccccc gagaggacgt tctcgacctt ccgcccctgg
     1141 cccatcgtga agaggcggaa gggaaggtcg accgtgccgt aggggtcgtg gcgcgagtag
     1201 gtcatcccgt gcgagcgagc gaagagcgcg aacgcctccc gccgcttctt ggcgaagtac
     1261 cagctgaggc cgcccacgac ggcgacgccc acgacgccga ggatgaagat caggaccgga
     1321 tccacgactc cagtatcgga gggtcatcga cgcaggtaaa ggctggggcg ggctttgtgg
     1381 gcggcgcggc ggaggttcct caacccggac agccttgggt cgcctgtggc tcctcgttag
     1441 tctcgcggcg acgccgtagc cgccgcgggg ctgcgccccc cttcctcagc gacgcttccg
     1501 gatccgcagg atagggcgca acagaaggat ttcctcgtgc atctgttcat acgggaggca
     1561 gacctcggct gtcggctgga aaggggaggc gaagatgcgg aggcttttgc gacctcgcga
     1621 tctggcgcgt tcgagccggg cgggcaaggg gttgggcgca ctcgcactgg cgcttcttgt
     1681 cgcggcggca tcctgcgggg gagacggata cgaggcctcc gagctcccgg cgatcgtgct
     1741 ccaggcgagc gaagccccag aggggaccgt gcatgagcga gatgagtcag gggcgcgtgc
     1801 gctgcgcgac ttctccgtcg acggccagga gaggctgcag ggcgaggggt tcgtcgacgg
     1861 gtatgcgacg gtcttcggcc caactggcgg ggtgaccacc catctcgaag ttacgtctgg
     1921 agcgatggtc ttcgaggaca gcagcagggc cgaggcgggg atggcagtcg tcctcgagga
     1981 tgtcgagggc cgactgaccg aggcgacgga gatatccgcg gacgggctgg gagctgagtc
     2041 ggcgggtctc tccggccccg tggacgggga cgccaggcaa cggagcgcgc tcctctacgt
     2101 ctggaagacc gacaacctcg tcctgttcgt gatcgcctcg gctctcctcg aaagcgcgcc
     2161 gagccctgac gtgatcctgg gcttggccga gacgatggac tcccgggcat cctgagttgg
     2221 gcgatcagtt ggccagactc gggtagccag cgcggtaagg acaccgtgcg gtcctgccac
     2281 ccggcgcagt gctggaattg agatcacaca gagtggtggc gcggccgaga agccggcggg
     2341 gggcgcaggc gcgccccccg tggctcgtcc ctcaccgatg cggctagatg cccagttctg
     2401 cgaacacctt ctgggcgcgt tcgagctgaa cctggatctc ctcgcccacg gtcagcatct
     2461 cgaccgtgaa gtgctccacc aggtagctgg tcttggtgag caggaccaag tactcctcgt
     2521 cttccatcag ctccaggacc ttctctcgag ggcccttgat caggatgaac cccgcctcgg
     2581 tctcgaggtc cgtcgtcctg gagaagtagg gctcgaagaa ggtgatcaca ccctccgtaa
     2641 gcttcttccc gaagaactcg atcgccgacg cgaagtgctc gatgccggcg cgctcccgcc
     2701 caggcaccag cttcccccag cggtacatca ggccgatctc atgcatctct gtcatctccc
     2761 cgcgctcaga ctcggcccgc cgggtcccca gcatcacctc cccgacccgt cgggaacggt
     2821 gacccacgaa ggacgattct gggccgagtg ccccggaggg gatagggtcg aagggcccta
     2881 gccggcaggg accaggtgcc gcccgaagga gctaacgtgc cggcgtgcgc cttctcgatc
     2941 ttgtcgagca ggacaaccga gtacgctcgc gccggacggt ctcggtaggc cggccgccct
     3001 cctcgtagcc cgcccagccg gcgcgtcgcc aacacagccc ggagaggcct tggccgggaa
     3061 gcgctagggg gggttcgcct cctcttcgat ccctgcggcg ggcacgccga ggatcaggtt
     3121 gagcgcaccg gcgatggcca ggccgagccc tgcgccgccc accacgtcga gcgggttgtg
     3181 cgctccgagg tacacgcgcg cgaagcagac caggcctacg agcgcccacg gaagcgccct
     3241 ccaggccccc cggaggtacg gggtgaccac cccggcgagg gccgccgtga gcacggcgtg
     3301 gccggagacg aaggcgagcc cgcggatggg caccccgcgg gcgatggcgt cgggcgtgga
     3361 cacggccggc cggggacgct ccacgaccag cttcacgacc cgttccagcg ccagcttcag
     3421 gacggtggcg aggaccgcgg cgacggcgag ccgcagcctc cggaacccca gcgcgatgac
     3481 cgcgaccgcc ggtcccacca gcaggacccc caggatctcg gcggcctgca tcggcaccct
     3541 gagccacgag ggaagcccgt tgatcgcgtg gaagaccgcc tgttcccatc cgggaacggt
     3601 cccgccgctc gcgaccgccc ccgtcagcag gagcgtgccg aggccggcca cgatccagac
     3661 ggcatcgagg cgacgccgcc tgacgggacg acgggacgat ggggcccgca ccagggcacc
     3721 ttatccaccg gtcgcccgcc ccgctcaccc acgccacatc gatgaccaca tcaacccaac
     3781 ctactaggag ccatgggaac ccatgctcca gaaatcacat ccaagaaaag gatgtgctag
     3841 gcctgaggga gagaggaggt ggcggtattg acggcgagat cagcgacgat cgcggtggcg
     3901 atagcgttgc tcctgggggg gtgcgtacag agcgatcgtg gctctccctc cttggagctt
     3961 caccgcgtgc agccgtcaac gcgtgagatc accccagcct tcctcttctt catgggacag
     4021 ttcaagaacg accgctctcc cttcgcgact ccggctgacc tcgacgctgc cgcagacgcc
     4081 gtcgtggtag gcacgatcgt ggctgtgcgg cctgggcaat cctccgcccc agctgtcgaa
     4141 agtgatcctg ttgtcgcgac ctcggtactg gaggtgaagg tcgagagcat cgttcgacaa
     4201 gatctcgagg tcgcgcgcgc cgggagcgtt tacgtggagg tgcctcatcc cgcctatgtg
     4261 ggtcccggcg aggctgggcc caccggtgag gggaccgacg aacggcaccc gttcgaccac
     4321 gcggcgttcg cagcgaccgt gccaagagca tacggcgtgt tcttctcgac gatcgaacac
     4381 aggagccata ctgggacacg atcctggagg cgggcgctgg cagaccaacc ggctccagga
     4441 tcacgaccgc gtacgttcaa ggtttcctga tcgaggatgc caacggcagt ctgatcagcg
     4501 ccctggagcg cttcgaggac ttgccgcacg cctggcgaag cctcgattcc gtcgaggatg
     4561 tcctagttct gatcgacgag ccataggcct cccgatcctc tctacggctt ccccaaccga
     4621 ggcgttccct cgtcccatcc tcaggccttc tgcaccacaa gcccgtcgcc cgagcggtcc
     4681 acgacgaggt ggtcgccgtc ggtgatctcg ccttcgagca gagccagcgc gagcttgtcc
     4741 tggatctcct tctggatcag tcgcttcagc ggccgggcgc cgagggcggg gtcccacccc
     4801 cgctccgcca ggtagctccg ggcatcgtcg gtcagcttca gcgtgagtcg ccgctcggcg
     4861 agccgctccg cgaaccgccg cacctggatg tccacgatct cggcgatctg ctccttgtcc
     4921 agcgggtcga agacgatcac gtcgtccacg cggttcagga actcggggcg gaaggcctcg
     4981 cgcatctcgg ccagcacctg ctcgcgccgc tgctccggcg agagcgtctc ctcgaagaac
     5041 cagcggctcc ccaggttcga ggtcatgatc acgacggcgt tcttaaagtc caccgtgcgg
     5101 ccctggccgt cggtgaggcg gccgtcgtcg aggacctgga gcaacacgtt gaacgcgtcg
     5161 gcgtgagcct tctcgatctc gtcgagcagg atcaccgagt aggggcggcg gcggacggcc
     5221 tcggtgagct ggccgccctc ctcgtagccc acatagccgg gaggcgcgcc gatcaggcgg
     5281 gccacggcgt ggcgctcctg gtactcgctc atgtcgatcc gcaccatcgc ccgctcgtcg
     5341 tcgaagagga actcggcgag ggcccgagcg agctcggtct tccccacccc ggtgggaccg
     5401 aggaagatga acgagccgat cgggcgattg gggtccgaga ggccggcccg ggagcgccgg
     5461 atcgcgttgg ccacggccgt caccgcctcg tcctggccca cgacgcgctc gtgaagggcc
     5521 tcctccatgc ggacgagctt ctgcatctcg ccctccagga ggcgcgagac cggcacgccg
     5581 gtccatgccg agacgacctc ggccacgtcc tcctcgtcca ctcgctcctt cagcatcttg
     5641 cggtcggcct ggacctcggc cagcgtcgcg ttctcggcct cgagttcctt ctccagctcg
     5701 acgatccggc cgtagcggag ctccgcggcg cgctccagat cgccgtcgcg ctcggcgcgc
     5761 tcggcctggc ccctcgtctc ctcgagctcg gacttcagct cgtggatccg cgagatgtgc
     5821 tccttctccc ggtcccagtg ggcccgcatg gcgctgagct gctcgttcag gtcggccagc
     5881 tcctgctcga tcttctccag gcgctgtttc gacgcctcgt cggtctcctt cttcagcgcg
     5941 gcccgctcga tctcgagctg cttgacgcga cggtccacct cgtcgatctc cacgggcatc
     6001 gagtcgatct cgatccggag gcggctggcg gcctcgtcca ccaggtcgat cgccttgtcg
     6061 gggaggaagc gcccggtgac gtagcggtcg gaaagcatcg cggcggccac gagcgcctcg
     6121 tccttgatct cgacgccgtg gtggacctcg tagcgctcgc ggaggccgcg caggatcgcg
     6181 atcgtgtcct cgaccgaggg ctcgccgacg aagaccggct ggaaccggcg ctccagcgcg
     6241 gcgtccttct ccacgtgctt gcggtactcg tcgagggtgg tggccccgat catgcggagc
     6301 tcgccgcggg ccaggagcgg cttcagcatg ttgcccgcgt cgaccgcgcc ctcggcggcg
     6361 cccgcgccca cgatcgtgtg cagctcgtcg atgaaggtga tgatctgtcc ctcgctggcg
     6421 gcgatctcct ggagcacggc cttgaggcgg tcctcgaact cgccccggta cttggcgccg
     6481 gccaccatgg ccccgatgtc gagggccacg atgcggcggc ccttcagcga ctcggggacg
     6541 tcgccggcca cgatgcgctg ggcgagcccc tcggcgatcg cggtcttccc cacgccgggc
     6601 tcgccgatca gaacggggtt gttcttcgtg cgtcgcgaga ggacctggat cacgcggcgg
     6661 acctcctcgt cgcggccgat caccgggtcg agcttgcctc ggcgggccag gtcggtcagg
     6721 tcgcgaccgt agcgctcgag ggcctcgtac ttctcctcgg ggttctcgtc ggtgacgcgc
     6781 tggcggccgc ggacctcggc cagcgcggcc aggaggcggt cgcgatcgag gccgagctca
     6841 cggaggagct cgccgagcgg gccctggtcg ccgagcatcg ctagcaggag gtgctcggtc
     6901 gagacgtact cgtcggtgag ggcctccgcc tcgtcggcgg ccgcctcgag gacgcgggcg
     6961 gtggccgggg cgagtcgcgc atcttgcgtg gtgccgtaga ccttcgggat ctggtcgaga
     7021 cgctcctcga cgcgcccggc caccgcccgc ggcgagagcc ccacccggtg cagcagcggg
     7081 tagaccacgc cctcgggatc ggcgagcagc gcaacgagga cgtgctcggg ctcgaccgcc
     7141 tggtggttgc ggtcgcgggc ctctcgctgg gcggtgccca gcgactcgcg ggaccgcacg
     7201 gtcagtctgt tcgggtcgaa cgccatgtca tcgctcccag ggaaggaccg tgctctcgta
     7261 cagcacgagc tcgttgttgc ggcggcgctc ctcgcggagg cggcgccgca gcccctcgat
     7321 cgcctgcttc gtctcctcga gctcgtcctg aagctcgtcg atctccgcct gcatggcgag
     7381 gatcctcttc acgccggcca ggttgacgcc ggcctgggtc atctcctgga tcgtccgcag
     7441 ccgggcgatg tcccggtcgg agtagcgtcg cgtgttgccg gccgtccgcg aggggcgcac
     7501 gagacccttc cgctcgtaga tccggagggt ctggggatgc acgccggcca gctcggcggc
     7561 cacgctgatg atgtacaccg cctgctccct cttccggtcc cgccgctccg ccatcgctca
     7621 gctcacctcg ctcacgacgc cctcggccga gggccctcac ccgacacctc gcggagctgc
     7681 tccagcagct cgcgctcctt cttcgacact ttcgaaggca cggccacctt gactgtggcc
     7741 aggaggtcgc ccttgccgcc acgtcgtttg gggatgcccc gcccccggat ccgaaaggtc
     7801 ttcccggagg gcgtgcccgc cgggaccttc agtgtgacgg gcccgttgag cgtggggacc
     7861 tcgaccttcg cccccagcgc cgcctccggg tacgtcaccg ggatctccac ggtcaggttg
     7921 ctgcccttgc ggccgaagcg ctcatggggc gacacgcgga cgaccacgta gaggtcgccc
     7981 gggcggccgc cggggcctcc gggctcgccg cggcccgcga gccggatccg ggcgccgtcg
     8041 tccacgcccg cggggatcct gacccggagc gtccgggtcc gacgctgggt cccgctcccc
     8101 cgacaggtgg ggcagggctc ctcgatctcc cggccgctcc cgccgcaccg cgggcatggc
     8161 tgagcgatcg agaacatccc ctggtcgatg gcgacggtgc ccgagccccc gcactgggtg
     8221 caggtgcgaa cgctggtgcc cggcttggcg ccggaaccgc cgcacatctc acacggcgcg
     8281 ggaccggcca cgcggacggg gaccgtggct ccctccatcg cctggtcgaa cgagatccgg
     8341 acctcggtct cgaggtcgac accccgctgt ggccctcgtc gggggccggc ggcgcccgcg
     8401 ccgaagccac ccccgaatag gtcgccgaag aggtcgccga acccgccgag gtcttcgaac
     8461 cgcacgcggg cgccgccggc ccccccgggc ccgccgccga agccgccgcc gaagccggcg
     8521 gaggccatct cgcgtacgcg gtcgtactcg gcccggcgct tctcatcccc caggacgtcg
     8581 taggccgcgg agacctcctt gaaccgctcc tccgcctcct tgtcgcccgg gttcgcgtcc
     8641 gggtggtgct tctgggccag ccgccggtag gccttcttga tctcggcctg tgtcgcgctc
     8701 ttggaaacac cgagcgcggc gtagtagtcc ttctcgagcc actcgcggct ggccgccacg
     8761 gctcagacct cccggcgctc gaccttcacg cccgcgggac ggacgacctt ccccttcagc
     8821 gtgtagccgg tccggaggac atcggcgacg taggggtcgc cgtcctcctc cccgccctgg
     8881 aagagcgcct cgtgcagctc gggatcgaag agcttgccct tcgcctcgat cctctccagg
     8941 ccctcggacc gaaggacgtc caccagcttg gcgtagacca tctccacgcc ctggaggaac
     9001 ttctcgaagt cgggcctctg ctcggcggcg acgagcgcga gctcgaagtc gtcgacgacc
     9061 gagagcagct tcaggacgag gtcctccgag gcgagctcga tggcgcgtgt ctgctccttc
     9121 agaacccgct tgcggtagtt ctcgaactcg gccttgaggc gctggaggtg gttgaggtac
     9181 tcggccgcct gctcccgggc ctcgtcgagc tccgaggtat cgggggcctc ctcgacctcg
     9241 ccctcgacga cctcctcgac gacgtcatcg ggccgctgct cgtcggtcac gagctacccc
     9301 cttcgtccac gacctcgccc tcgacgacgt cctcctcgcc ctcgggggcc tgggcctcgg
     9361 cgccctcggc cccgccggcg gcggcctgct cctgctgata cacagcctgg cccaccttct
     9421 ggaaggcttc cgtcagggcc tcggtcttcg ccttgatttc gtcgaggtcg tcgccctcga
     9481 gcgccttgcg gacgtcggcc accgccgtct ccacggcctg acggtcgtcg tcggggacct
     9541 tgtcg
//
LOCUS       DASUTZ010000105        10380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_675754, whole genome shotgun sequence.
ACCESSION   DASUTZ010000105 DASUTZ010000000
VERSION     DASUTZ010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10380
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675754"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="VF097_04735"
     CDS             <1..960
                     /locus_tag="VF097_04735"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX6262135.1"
                     /translation="SEPLGVAIAGTVIVALGAFDDVRGMAAPAKVAGQILAGGILILF
                     GVQLVYFWLPGIGLLSLSPDLAVPLTLLWVLAMVNALNLIDGLDGLAAGVAAIAAGAF
                     FVYAFQAADPTFALESGSGGVSPAALFTALVVGAAIGFLPYNLHPARIFMGDSGSMLL
                     GLVLAAATVSGVGRTDAPANPDFAALAIPVMIPVLVLAVPITDVVLAVIRRLRRGRAI
                     FRPDKEHIHHRLLEIGHSHRTAVLLMYLWSSLLAASALAITYIRSRALAIVLLAAVLL
                     ILVGTAVPRVFRANGRERPLRASPEATPENGERSGRRSRSSRP"
     gene            1000..2172
                     /locus_tag="VF097_04740"
     CDS             1000..2172
                     /locus_tag="VF097_04740"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX6262136.1"
                     /translation="MDRSPLRVAVVAGLSAVVAVTGLAPASAGSAAGITLQAEPVPVR
                     FGESVTLSGVVDPPDEGPVTIVEVSTSDPVLSPVPSGPNGSFSVTFEPDRNVRLRAEW
                     NGEQSDPLWVRVRPIVRVKLGQLRLFGKAKVTGRVRPAIAGARVEVSLRRGVKTIARR
                     KVPLTAEGRFTARLPVRKPGRLRALAVFDHEDLLRASARSRPRKTPLPSLGQGSRGKV
                     VRLLEQRLRSLGYWLTGINRAFDHRTGDAVLAFHKVQGMARVKSVRPATWRRLASPRR
                     PSPRSTKPAFHVEIDQTKQVLYVIRRGEISEIVHTSSGAGGATRDGVFRVHRKIAGYS
                     PGRLYYPSYFDGLRAVHGWPSVPTYPASHGCSRVPNWTAIHLHNVMPIGTVVRVYH"
     gene            complement(2177..2950)
                     /locus_tag="VF097_04745"
     CDS             complement(2177..2950)
                     /locus_tag="VF097_04745"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6262137.1"
                     /translation="MSEVRLAARQILYENRAFWRNPPAAFFTFAFPLIFLVIFNMLFG
                     NAEIDVPGGQTTASTFFVPAIATFAIVNATFTNLATSIPFARDQGLLKRYRGTPLPGW
                     AYLTARVAHAIVVTILLVGIVTAFGALFYGVDAPTNTLPGALVALAVGGFAFCSLGLA
                     MTAIIPNADAGPAIVNAVILPLLFISDVFVQTDQAPAWVRILGSVFPVKHLSEALQTA
                     FNPFHPGAGFEWGHVGIVAAWGVAGLILAIRFFSWEPRT"
     gene            complement(2947..3840)
                     /locus_tag="VF097_04750"
     CDS             complement(2947..3840)
                     /locus_tag="VF097_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6262138.1"
                     /translation="MSETAIEVRGLRKSYGHVEAVAGLDLTVHRGEVMALLGPNGAGK
                     TTTVEILEGHRDRDEGEIWVLGHDPEERSPDLRRRVGIVLQTTGVEPYLTVGETIEQF
                     RGFYPSPLPLDEVLERVGLADLRDRRVKRLSGGQQRRLDVAIGLAGDPELLFLDEPTT
                     GFDPSARRGAWGMIRNLRSLGKTVLLTTHYMDEAQSLADRVAILVQGRIVAEGPPAIL
                     TGDNRLTTIRFRRSAEDLPPEVGEPEIEDGMVRIRTSEPTRVLHVLTAWATARGEELH
                     ELTVAKPSLEDVYLELTERSS"
     gene            3921..5090
                     /locus_tag="VF097_04755"
     CDS             3921..5090
                     /locus_tag="VF097_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012786399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6262139.1"
                     /translation="MDFDLTEEQQEFRKLVRQFAEEVVAPRVEEMDQAGELPMDIVKQ
                     MAEMGLFGLPFPEEYGGQGADFLTLCIAIEELARVDSSIAITLEAAVGLGASPIYHHG
                     TEEQKQRWLVPMARGEILGSYGLTEPGGGSDAEATRTTARLEDGEWVINGSKAFITNS
                     GTPISRVCTITAVTGEAEDGGKEISSIIVPTDTPGFEIGKSYRKVGWRASDTHELAFD
                     DVRVPEENLLGERGEGFPQFLQTLDEGRIAIAALAVGLAQGCLEESVRYAKEREAFGR
                     PIGAFQGMQFKIADMKVAAETARLATYRAAWLRDQGRPYKAEASLAKLYASEIAVTSA
                     REAVQVHGGYGFIEEFPVARFYRDSKVLEIGEGTSEIQRVLIARDLGLPGTEFGD"
     gene            5165..6112
                     /locus_tag="VF097_04760"
     CDS             5165..6112
                     /locus_tag="VF097_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX6262140.1"
                     /translation="MAAESRTTVLAALFANAVIAVAKLIGGLISGSSAMLAEAAHSMA
                     DTTNQGLLLMSISLGKRKPDERHPFGYGKERFFWAFIAAVFIFVAGSAFSLYEGVRAI
                     GAPHGEGEAGFAIPYAVLGVALVLEAIALWVAYRGVRREAARRRRRFFSFLRVTRDPT
                     AKTALFEDAAAVAGVMVALVGVGLHQLTGDPRWDAGASIVIGVILAIVAFVLARDTKA
                     LLLGEPAFPEERELLRECMLSEDGVEHVVELLTMAMGPESLLVTARLDLREGLDEDAI
                     EALAHSIDRRCREAVPAVKELFLDPTSRDEIPIQDDGGL"
     gene            6215..7375
                     /locus_tag="VF097_04765"
     CDS             6215..7375
                     /locus_tag="VF097_04765"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX6262141.1"
                     /translation="MNSLSRRRQGFLRLRRGLALAVGLMLLAQVAPGSAQTTEEKLQA
                     AKKRMAQIQRQLDRERAKLDQLNAEIAGINRRIAQAYAQRIAIQDQIKVTEETIQRKG
                     RRIRALQGRLDERAREVYIQGPAGMLEFVLEAESLSDLSDRVSFLSALSEGDASTAIG
                     IEVEREELGRFEDDLGVLLEEQDALLAELRQQEEQLNARWDEQARIEASIADKLEEAE
                     ALVKRLKKQRRRELLAALAAARGNVRPVDASGLLKWCPVDRPRSYIDDFGFPRSGGRT
                     HQGNDMFAPAGTPIRAPFAGVADESWNTLGGYSVFVRAADGTYVYNAHLSRYAGVDGK
                     QVAPGDLIGYVGNTGNAVGTPPHDHFELHPGGGSAINPYPFLNAVCGVNGGG"
     gene            7403..9400
                     /locus_tag="VF097_04770"
     CDS             7403..9400
                     /locus_tag="VF097_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein meaA"
                     /protein_id="HEX6262142.1"
                     /translation="MPERDRPWIYRTYAGFGTPAVTNERFRANLAKGQTGLSIAFDLP
                     TQCGYDPGHPMSAGEVGRVGVPIAHLGDMRTVLDGIPVAEMNTSMTINATAVWLLALY
                     VAVADEHGASRAELRGTTQNDILKEYLSRGTFIFPPEPSMRLTTDLITWTAREAPAWN
                     PINVCSYHLQEAGADPVLEVAFTLANAAAVLDATRATGAVPAEDLPRVVGRVSFFCNA
                     GIRFVEELSKMRAFVELWDRLTRERYGVEDPRMRRFRYGVQVNSLGLTAAQPENNVHR
                     ILLEMLSVTLSRDARARAVQLPAWNEALGLPRAWDQQWSLRAQQILAEETDLLEHGDL
                     FVGSTVVAERTDEIATAAWEELGRVLDRGGSAEALAYMKERLVAKHAERLRAIEEGEL
                     TLIGVNRYTETEVSPLVEGLGEGFASFHEVNPELEAEQAESVERWRAERDGAAAARAL
                     DALRAAAASGENLVPVSIEAARAGVTTGEWGAALREVFGEFRAPTGVSGASGVGADAE
                     SLREARARVEEAARRAGVARLRMLVAKPGLDGHSNAAEQVAVRARDAGFEVVYQGIRL
                     TPEQIARAAAEEDVHVVGLSVLSGAHNLIVPKVLERLREEGVDPSRVPVVVGGIIPDD
                     DAAKLSELGVARVYTPRDFDLTRQVGEIADLIAASADAGGR"
     gene            9397..10182
                     /locus_tag="VF097_04775"
     CDS             9397..10182
                     /locus_tag="VF097_04775"
                     /inference="COORDINATES: protein motif:HMM:NF004869.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HEX6262143.1"
                     /translation="MSEERRPTEKPWRTAIGKVAPNEIRVRGYDLMELIGTRSFGDVT
                     YLLLSGELPEGNEGRMVEAMLVSAAEHSIVAPSVAAARYIASTGVPLQAAVSVGVAGM
                     GDFHGGAVEAGALMLREAADLSSDPSEAADGIVRAFRERGERIPGYGHVVHDPDPRGR
                     RLLEVAGDLGLRGRWCVIAQEIEPATERHLGRRLAMNVDGAMAALLLELGLDPGLGKA
                     FYVIGRTPGLVAHVHEERTRERPYRDPGWENVEYDGPPPRDLP"
     gene            10240..>10380
                     /locus_tag="VF097_04780"
     CDS             10240..>10380
                     /locus_tag="VF097_04780"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX6262144.1"
                     /translation="MKPEDVKKVAVIGTGIMGSGIVEVCAKADCEVVTVEVDDQAMDR
                     GRQ"
BASE COUNT         1489 a         3538 c         3861 g         1492 t
ORIGIN      
        1 tccgagcccc tcggcgtggc gatcgccggc accgtgatcg tggcgctggg cgccttcgac
       61 gacgtgcggg ggatggcggc cccggccaag gtcgccgggc agatcctggc cggggggatc
      121 ctgatcctct tcggcgtcca gctcgtgtac ttctggctcc ccgggatcgg gctgctctcg
      181 ctctcgccag acctggccgt gccgctcacc ctgctttggg tcctggcgat ggtcaacgcg
      241 ttgaacctga tcgacggcct ggacggtctg gccgcagggg tggccgcgat cgcggcgggg
      301 gccttcttcg tctacgcgtt ccaggccgcg gaccccacgt tcgccctcga gtcggggagc
      361 gggggcgtct cgccggcggc gctcttcacg gcgctcgtgg tgggggccgc gatcgggttc
      421 ctgccctaca acctccaccc ggccaggatc ttcatgggcg actccgggag catgctgctg
      481 gggctggtcc tcgccgccgc gaccgtctcg ggggtggggc gcaccgacgc gccggccaat
      541 cccgacttcg cggccctggc catcccggtg atgatccccg tgctcgtcct ggccgtcccc
      601 atcaccgacg tggtcctggc ggtgatccgg aggctccggc ggggtcgcgc catcttccgg
      661 cccgacaagg agcacatcca ccaccgcctg ctcgagatcg gccactcgca ccgcaccgcg
      721 gtcctgctca tgtacctgtg gagctccctg ctcgccgcct cggcgctcgc gatcacctac
      781 attcggagcc gggcgctggc gatcgtcctg ctcgcggccg tcctgctgat cctggtgggg
      841 acggccgtcc ctcgcgtgtt cagggcgaac gggcgggagc gacccctccg ggcgtcgccc
      901 gaggccacac ccgagaacgg cgagcggtcg gggcgccgca gccgcagctc tcgcccctag
      961 cccgtggact cgcggatcgg ctcgtgctat caacgctgga tggatcgcag ccccctccgc
     1021 gtcgccgtcg tcgccggcct ctcggcggtc gtcgcggtga ccggtctggc ccccgcctcg
     1081 gcgggttcgg cggccggcat cacgctccag gccgagcccg tccccgtccg gttcggcgag
     1141 agcgtcacgc tgtcgggcgt ggtcgacccg cccgacgagg gtccggtgac gatcgtcgag
     1201 gtgagcacga gcgaccccgt gctgagcccc gtgccctcgg gcccgaacgg ttccttctcg
     1261 gtcacgttcg agcccgatcg gaacgtgcga ctgcgggccg agtggaacgg cgagcagagc
     1321 gacccactct gggtgcgcgt tcgtccgatc gtccgcgtga agctgggcca gctgcgcctc
     1381 ttcgggaagg cgaaggtgac cgggcgggtc cgcccggcga tcgcgggggc gcgggtggag
     1441 gtctccctgc gccgtggggt caagacgatc gcccggcgga aggtacccct gacggcggag
     1501 ggacggttca cggcgcggct gccggtgcgg aagccggggc ggctccgcgc gctcgccgtg
     1561 ttcgatcacg aggacctcct gcgggcgagc gcgcggagcc gcccccggaa gacgccgctc
     1621 ccctcgctgg gacagggctc gcgagggaag gtcgttcggc tcctcgaaca gcggcttcgc
     1681 tcgctcggct actggctcac ggggatcaac cgcgcgttcg atcaccgcac gggggatgcg
     1741 gtgctcgcct tccacaaggt acaggggatg gcccgggtga agtccgtccg cccggccacc
     1801 tggcggcggc tcgcgtcgcc caggcggccc agcccccgct ccacgaaacc cgccttccac
     1861 gtcgagatcg accagacgaa gcaggtcctc tacgtgatcc ggcgggggga gatcagcgag
     1921 atcgtccaca cctcgagcgg ggccggcggc gcgacccggg atggcgtgtt ccgggtccat
     1981 cggaagattg cggggtacag cccgggccgt ctctactacc cgtcgtactt cgacggcctg
     2041 cgcgcggtgc acgggtggcc gtcggtgccg acctacccgg cgagccacgg gtgctcgcgg
     2101 gtcccgaact ggaccgcgat ccacctgcac aacgtcatgc cgatcggcac ggtggtgcgc
     2161 gtctaccact gacgcgctag gtccgcggct cccaggagaa gaagcggatc gccaagatca
     2221 gcccggccac gccccacgcc gccacgatcc ccacgtgacc ccactcgaac ccggccccgg
     2281 ggtggaacgg gttgaacgcc gtctggaggg cctcggagag atgcttcacg gggaagacgc
     2341 tgcccaagat tctcacccag gcgggggcct ggtcggtctg gacgaacacg tccgagatga
     2401 agagcagcgg caggatcacc gcgttcacga tggcgggtcc cgcatccgcg ttcgggatga
     2461 tcgccgtcat ggccagtccc agtgagcaga acgcgaaccc gccgaccgcg agcgccacca
     2521 gcgcgccggg caaggtgttc gtcggggcgt caacgccgta gaacagggcg ccgaacgccg
     2581 tgacgatacc caccagcagg atcgtgacga cgatcgcgtg cgcgacgcgc gcggtgaggt
     2641 aggcccaccc cgggagcggc gtcccccggt agcgcttcaa caggccctgg tcgcgggcga
     2701 acgggatcga cgtggccagg ttcgtgaacg tggcgttgac gatggcgaag gtggcgatcg
     2761 cggggacgaa gaaggtcgac gcggtggtct gccctccggg cacgtcgatc tccgcgttcc
     2821 cgaacagcat gttgaagatc acgaggaaga tcagcgggaa ggcgaacgtg aagaaggccg
     2881 ccggcgggtt ccgccagaag gcgcgattct cgtagaggat ctggcgcgcc gccaggcgaa
     2941 cctcgctcac gaggatctct ccgtcagctc gaggtatacg tcctccaagg agggcttcgc
     3001 caccgtgagc tcgtgcagct cctcgccccg cgcggtggcc caggcggtca gcacgtgcag
     3061 cacgcgggtg ggctcgctcg tgcggatccg gaccatgccg tcctcgatct cgggctctcc
     3121 cacctcgggc ggcaggtcct ccgccgatct ccggaacctg atcgtggtca gccggttgtc
     3181 gcccgtcagg atcgccggcg gcccctcggc cacgatccgc ccctggacga ggatcgccac
     3241 gcggtcggcc aggctctgcg cctcgtccat gtagtgggtg gtgaggagca ccgtcttccc
     3301 cagcgatcgg aggttccgga tcatgcccca ggctccgcgg cgggccgagg gatcgaagcc
     3361 ggtcgtgggt tcgtccagga agagcagctc cgggtccccg gccaggccga tggccacgtc
     3421 gagccgccgc tgctggccgc cggagagccg cttcacgcgg cgatcgcgga ggtcggccag
     3481 cccgaccctc tccaggacct cgtccagggg gaggggtgac ggatagaagc cgcggaactg
     3541 ctcgatcgtc tcccccacgg tcaggtacgg ctccacgccg gtggtctgca ggacgatacc
     3601 gacgcggcgt cggagatcgg gcgatcgctc ctccgggtcg tggcccagga cccagatctc
     3661 gccctcgtcg cggtcccgat gcccctcgag gatctccacg gtggtcgtct tcccggcgcc
     3721 gttgggcccg agcagcgcca tgacctcacc gcgatggacc gtcaggtcga gccccgcgac
     3781 ggcctcgacg tgcccgtagg acttccgcag cccccgcacc tcgatcgcgg tctcgctcat
     3841 gcttgcaggg tagcgcgagg ggttcgcggg ctccacctcg ggccctccgg tagactcgat
     3901 ccgacctcag gagggaccgg atggacttcg atctgaccga ggagcagcag gagttccgca
     3961 agctcgtccg ccagttcgcg gaggaggtcg tggcgcctcg cgttgaggag atggaccagg
     4021 ctggcgagct cccgatggac atcgtcaagc agatggccga gatgggcctc ttcgggctcc
     4081 cgttccccga ggagtacggc ggccagggcg ccgacttcct cacgctctgc atcgcgatcg
     4141 aggagctggc ccgcgtggac tcctcgatcg ccatcacgct cgaggcggcg gtcggcctcg
     4201 gggccagccc gatctaccac cacggcaccg aagagcagaa gcagcgctgg ctcgtcccga
     4261 tggcgcgggg ggagatcctc ggctcgtacg ggctcaccga gcccgggggc ggctccgacg
     4321 ccgaggccac caggacgacg gctcgcctcg aggacggcga gtgggtgatc aacggctcca
     4381 aggccttcat cacgaactcg ggcacgccga tctcccgcgt ctgcacgatc accgccgtca
     4441 ccggcgaagc cgaggacggc ggaaaggaga tctcctcgat catcgtcccc accgacaccc
     4501 ccggcttcga gatcggcaag tcgtaccgga aggtgggatg gcgagccagc gacacccacg
     4561 agctcgcctt cgacgacgtc cgcgtgcccg aggagaacct cctcggggag cggggagagg
     4621 gcttccccca gttcctccag accctcgacg aggggcggat cgcgatcgcc gcgctggcgg
     4681 tcggcctggc ccaggggtgc ctggaggagt cggtccggta cgcgaaggag cgcgaggcgt
     4741 tcggtcggcc gatcggagcg ttccagggga tgcagttcaa gatcgccgac atgaaggtgg
     4801 cggccgagac ggctcgcctc gccacctaca gggcggcgtg gctccgcgac cagggccgcc
     4861 cgtacaaggc cgaggcctcc ctggccaagc tgtacgcctc ggagatcgcg gtcacctccg
     4921 cccgcgaggc ggtgcaggtt cacggcggct acgggttcat cgaggagttc cccgtggctc
     4981 gcttctaccg cgactcgaag gtcctggaga tcggcgaggg gacgagcgag atccagcggg
     5041 tcctgatcgc ccgggacctg ggccttcccg ggacggagtt cggcgattga tgcccgtccc
     5101 cggggcctcg ctgtggctcg tgcgggacga ggagagccgg cgcacgtagc cgggggagag
     5161 ctggatggcc gccgagagca ggaccacggt cctggcggcg ctcttcgcca atgccgtgat
     5221 cgccgtggcc aagctcatcg gcgggctcat ctcggggtcc tcggccatgc tggcggaggc
     5281 cgcgcactcg atggccgaca ccacgaacca gggtctgctc ctcatgtcga tctcgttggg
     5341 gaagcggaag cccgacgagc ggcacccgtt cgggtacggc aaggagcgct tcttctgggc
     5401 gttcatcgcg gcggtcttca tcttcgtggc gggttcggca ttctccctct acgagggcgt
     5461 gcgggccatc ggcgctcccc acggcgaggg cgaggcgggc ttcgcgatcc cgtacgccgt
     5521 cctgggcgtg gcgctcgtct tggaggcgat cgccctgtgg gtggcctacc gaggggtgcg
     5581 acgggaggcg gctcggcgcc gccggcggtt cttctcgttc ctccgcgtga cgcgggaccc
     5641 gacggcgaag accgccctgt tcgaggacgc ggcggcggtg gccggcgtga tggtggccct
     5701 cgtgggcgtc ggactccacc agctgaccgg cgatccgcgg tgggatgccg gcgcctcgat
     5761 cgtgatcggc gtgatcctgg ccatcgtggc cttcgtgctg gcccgcgaca cgaaggcgct
     5821 gctgctgggg gagcctgcct tccccgaaga gcgggagctc ctccgcgagt gcatgctctc
     5881 cgaggacggg gtggagcacg tcgtcgagct cctgacgatg gccatggggc ccgaatcgct
     5941 gctcgtcacg gcccgcctgg acctgcgcga gggcctggac gaggacgcga tcgaggccct
     6001 ggcccactcg atcgaccgcc ggtgtcggga ggccgtcccc gccgtcaagg agctcttcct
     6061 ggacccgacc tccagggacg agatcccgat ccaggacgac ggcggattgt agtgggcggc
     6121 ggtttcggcc gatacttgct caggcggccg gatgccgtca cgctgggaac agcggttgac
     6181 agtggcaaca tgattcctgt agaggtagcc gttcgtgaac tcgctgagca ggcgccgtca
     6241 aggcttcctc cgcctgcggc ggggcctcgc gctcgccgtc gggctgatgc tgctcgccca
     6301 ggtggctccc gggtccgccc agacgaccga ggagaagctg caggcggcca agaagcgcat
     6361 ggcccagatc cagcgccagc tcgatcggga gcgggccaag ctcgaccagc tcaacgctga
     6421 gatcgcgggc atcaacaggc gaatcgccca ggcctacgcc cagcggatcg cgatccagga
     6481 ccagatcaag gtcacggagg agacgatcca gcgaaagggc cgccgcatcc gcgcgctcca
     6541 ggggcgcctc gacgagcggg cccgcgaggt ctacatccag ggccccgccg ggatgctcga
     6601 gttcgtgctc gaggcggagt cgctctcgga cctcagcgac cgggtgagct tcctctcggc
     6661 cctgagcgag ggcgacgcca gcacggccat cgggatcgag gtcgagcggg aggagctggg
     6721 gcgcttcgag gatgacctgg gggtgctgct cgaggagcag gacgccctgc tggccgagct
     6781 gcggcagcag gaggagcagc tcaacgcccg ctgggacgag caggcgagga tcgaggcctc
     6841 gatcgcggac aagctcgagg aggccgaggc cctcgtcaag aggctgaaga agcagaggcg
     6901 ccgggagctg ctcgcggccc tggccgcggc gcgggggaac gtccgcccgg tggacgccag
     6961 cgggctcctc aagtggtgcc cggtggaccg gccgcgatcc tacatcgacg acttcgggtt
     7021 ccctcgctcc ggtggccgca cccaccaggg caacgacatg ttcgcccccg ccggcacgcc
     7081 gatccgggcg ccgttcgcag gcgtcgcgga cgagagctgg aacacgctcg ggggctattc
     7141 ggtcttcgtc cgggcggccg acggcaccta cgtgtacaac gctcacctct cgcgctacgc
     7201 gggtgtggac gggaagcagg tggcgcccgg cgacctgatc ggatacgtcg ggaacacggg
     7261 gaacgccgtg ggcaccccgc cccacgacca cttcgagctg catccgggag ggggctcggc
     7321 gatcaacccg tatcccttcc tgaacgccgt gtgcggcgtg aacggcggcg gctgagcggg
     7381 gggcggcggg tagactcagc gcatgcctga gcgggatcgt ccctggatct accgcaccta
     7441 cgcggggttc gggacgccgg ccgtcacgaa cgagcgcttc cgggcgaacc tggccaaggg
     7501 acagacgggc ctctcgatcg ccttcgacct tcccacgcag tgcggctacg acccgggcca
     7561 cccgatgtcg gcgggcgagg tgggccgagt gggggtcccg atcgcgcacc tcggggacat
     7621 gcgcacggtg ctggacggca tcccggtggc cgagatgaac acctcgatga cgatcaacgc
     7681 cacggcggtg tggctgctcg cgctgtacgt cgcggtggcc gacgagcacg gggcctcgcg
     7741 cgcagagctc cgaggcacga cgcagaacga catcctgaag gagtacctgt cgcgggggac
     7801 gttcatcttc ccgccggagc cctcgatgcg gctgacgacc gacctgatca cgtggacggc
     7861 gcgcgaggcg cccgcctgga acccgatcaa cgtgtgctcg taccacctgc aggaggcggg
     7921 cgccgatccc gtgctcgagg tggcgttcac gctggccaac gccgcggcgg tgctcgacgc
     7981 gacacgagcg acgggggccg tgccggcgga ggatctcccg cgcgtggtgg ggcgcgtctc
     8041 gttcttctgc aacgccggga tccggttcgt cgaggagttg agcaagatgc gggccttcgt
     8101 cgagctgtgg gaccgtttga cgcgggagcg gtatggggtc gaggacccgc ggatgcggcg
     8161 gttccggtac ggggtccagg tgaactcgct ggggcttacg gcggcccagc cggagaacaa
     8221 cgtccaccgg atcctgctcg agatgctctc ggtgacgctg tccagggacg cccgggcgcg
     8281 ggccgttcag ctcccggcgt ggaacgaggc cctcgggctg ccgagggcgt gggaccagca
     8341 gtggtcgctc cgagcccagc agatcctggc ggaggagacc gacctgctcg agcacggcga
     8401 cctgttcgtt ggatcgacgg tggtggccga gcggaccgac gagatcgcga cggcggcgtg
     8461 ggaggagctg ggccgggttc tggacagggg aggctcggcg gaggcgctcg cctacatgaa
     8521 ggagcggctc gtggcgaagc acgccgagcg gctccgggcg atcgaagagg gcgagctgac
     8581 gctgatcggt gtgaaccggt acacggagac cgaggtctcg cccctcgtgg aggggctggg
     8641 cgaggggttc gcctcgttcc acgaggtgaa ccccgagctg gaggcggagc aggccgagag
     8701 cgtggagcgc tggagggccg agcgggacgg ggcggcggcc gcgcgggcgc tcgacgccct
     8761 ccgcgcggcc gccgcctccg gcgagaacct cgtgcccgtt tcgatcgagg ccgcccgggc
     8821 cggcgtgacg acgggggagt ggggggcagc gctgcgcgag gtgttcgggg agttccgggc
     8881 ccccaccggc gtgagcgggg cctccggggt gggcgccgac gccgagtccc tgcgggaggc
     8941 gcgggcccgc gtcgaggagg cggcccggcg agccggcgtc gcgcgcctcc gcatgctggt
     9001 ggccaagccg ggcctggacg gccactccaa cgcggcggaa caggtggccg tccgggcgcg
     9061 cgacgccggc ttcgaggtcg tctaccaggg catccgcctg acccccgagc agatcgcgcg
     9121 ggcggcggca gaggaggatg tccacgtcgt gggcctctcg gtgctgagcg gggcgcacaa
     9181 cctgatcgtc cccaaggtgc tggagcgcct ccgcgaggag ggtgtggacc cctcccgcgt
     9241 gcccgtggtc gtgggcggca tcatcccgga cgatgacgcg gccaagctct ccgagctggg
     9301 tgtggcgcgc gtctacacgc ctcgagactt cgacctgacg cggcaggtgg gcgagatcgc
     9361 ggacctgatc gccgcgagcg ccgacgcggg cggacgatga gcgaggagcg gcgccccacc
     9421 gagaagccct ggcgcacggc gatcggcaag gtcgctccga acgagatccg cgtgcggggc
     9481 tacgacctga tggagctgat cggtacgcga tcgttcggcg acgtcaccta tctccttctg
     9541 tccggcgagc tgcccgaggg gaacgagggc cgcatggtcg aggccatgct cgtgtcggcg
     9601 gccgagcact cgatcgtcgc gccctcggtg gcggccgccc ggtacatcgc ctcgaccggc
     9661 gtgccgctgc aggccgccgt ctccgtgggt gtggccggga tgggggactt ccacggggga
     9721 gcggtcgagg cgggggccct gatgctgcgg gaggccgccg acctcagctc cgatccctcg
     9781 gaggcggccg acggcatcgt ccgcgcgttc cgcgagcggg gcgagcgcat ccccgggtac
     9841 gggcacgtgg tccacgaccc cgatccgcgc gggcgtcggc tgctcgaggt ggcaggggac
     9901 ctcggtctcc ggggccggtg gtgcgtgatc gcccaggaga tcgagccggc caccgagcgt
     9961 cacctcggcc gccgcctggc gatgaacgtg gacggcgcga tggccgcgct cctgctcgag
    10021 ctgggcctgg accccggcct ggggaaggcc ttctacgtga tcggccgcac gcccgggctc
    10081 gtggcccacg tccacgagga gcggacgcgg gagcgcccct accgcgaccc gggctgggag
    10141 aacgtcgagt acgatggccc gccccctcga gacctgccct gagcctttgg cacgccccgc
    10201 ggggcggggc taggctgtgc ccagacccaa ggaggacgaa tgaagcctga ggacgtcaag
    10261 aaggtggcgg tgatcggcac cgggatcatg gggtcgggca tcgtcgaggt ctgcgccaag
    10321 gccgactgcg aggtcgtgac ggtggaggtc gacgaccagg cgatggaccg gggccgtcag
//
LOCUS       DASUTZ010000106         5208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1322814, whole genome shotgun sequence.
ACCESSION   DASUTZ010000106 DASUTZ010000000
VERSION     DASUTZ010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322814"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="VF097_04785"
     CDS             <1..1023
                     /locus_tag="VF097_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018793133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX6262145.1"
                     /translation="FEPTERDGRLFGRGAADDKAGVMAHVAAVLAHDGKPPVTVTAFI
                     EGEEETGSENLPAFLEAHRDALQADVIVLADSTNWRVGQPSLTVSLRGLVDCTVEVRT
                     LGHSVHSGFVGGVFPDALTVLSRLLATLHDEEGSVAVEGLLAEEADPLDLTEEELREQ
                     AAAVPGLRAVGEGSLTSRLWTRPAISVLGIDAPRVQEASNTLVPAARAKISLRLAPGQ
                     DPQAAMEALTRHLEERVEWGAQVAVTPGAIAYPARIRAAGPAYDAVRRAFREAWGTEP
                     VDIGAGGTIPFVQAFMEALPDAAILVTGVEDPDTRAHGTDESLHLEDFERVCVAEALL
                     LRELAT"
     gene            1120..1752
                     /locus_tag="VF097_04790"
     CDS             1120..1752
                     /locus_tag="VF097_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HEX6262146.1"
                     /translation="MAIRVDPPRVEARASGRTICRLSPRDEARWRALADRAAPLIEAR
                     LPDGVVADRAVRQRHGWRLAPMRPALRRARRAAASLAREGAVVATDVEAFFPSVTPET
                     LERALLAVGAARGDAAAAASMLGGWAEQGRAGLPVGPAASSILANAVLLPVDLAVAPV
                     RFLRWVDDYLLPAEYPLERFDEALAVVGLRRSERKTVRGIGGVWQAPYGR"
     gene            complement(1753..2037)
                     /locus_tag="VF097_04795"
     CDS             complement(1753..2037)
                     /locus_tag="VF097_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262147.1"
                     /translation="MKRAERRLLKVIDELQALDREQRLLEGELEMHRSLRDDAARDAA
                     VHETPVERGNAYETEKDVRTFERALAGLAARRAALEAKRDRLMTRLGRMP"
     gene            complement(2034..2912)
                     /gene="hisG"
                     /locus_tag="VF097_04800"
     CDS             complement(2034..2912)
                     /gene="hisG"
                     /locus_tag="VF097_04800"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX6262148.1"
                     /translation="MLRLVIPKGSLEEQTLSLLEGADLRVRRGSSRDYHGRIEDERID
                     RVSLMRPQEIPRYIEEGFFDLGITGRDWVEETGADVEILLELTYAKGGPGRPVKIVLA
                     VAADAPWEKASDLPDGTKISTEYPELTKRRFAELGIDVRVFESYGATEAKVPEIVDAI
                     VDVSETGGTLRAHGMRIIDELMESEVLLIANHGAWEDPDKRRAMEDVRTLIQGAMEAK
                     GRVLIKLNVTREKLDNVLEVLPAMKAPTVSSLAEEDEFAIETVVEKATVNALIPLLKA
                     HGATDILEIPISKIVP"
     gene            complement(2915..3253)
                     /gene="hisI"
                     /locus_tag="VF097_04805"
     CDS             complement(2915..3253)
                     /gene="hisI"
                     /locus_tag="VF097_04805"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006235892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEX6262149.1"
                     /translation="MSERAVSSVADLHYDERGLIPAVVQDEATGDVLMVAWMNEEAVR
                     ATLDEGRTVFWSRSRGELWRKGETSGNVQHVREVRYDCDADTLLVRVHADGPACHTGE
                     RSCFYRRLEV"
     gene            complement(3250..4011)
                     /gene="hisF"
                     /locus_tag="VF097_04810"
     CDS             complement(3250..4011)
                     /gene="hisF"
                     /locus_tag="VF097_04810"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX6262150.1"
                     /translation="MTLARRLIPCLDVEAGRVVKGTRFVDLRDAGDPAELAARYDAEG
                     ADELVFLDITASVEGRGAMLDVVARAADQIFIPLTVGGGVRTEEDVNALLRAGADKVG
                     LNTHGVREPELLARCADRFGTQCVVIAIDAKRRNGGWEVVVEGGRTPTGLDAVAWAAR
                     ATADLGAGEVLLTSIDRDGTGDGYDLELLRAVGNAVEVPVVASGGAGALEHFAQAFTE
                     GHADAVLAASRFHFGEFTVPQVKEYLAARGIPVRR"
     gene            complement(4008..4712)
                     /gene="hisA"
                     /locus_tag="VF097_04815"
     CDS             complement(4008..4712)
                     /gene="hisA"
                     /locus_tag="VF097_04815"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEX6262151.1"
                     /translation="MIVIPAIDVREGKVVRLRHGRLQEVIVYADDPVETARGYAADGA
                     QWVHVVDLDAAFDVGDNRPVIADVTREADVPIQVGGGLRSLYLIEGVLAVGAARVVLG
                     TEAILDPGFLAECVERYGERVVVALDTDGDVVKVRGWTEPAGSFDQVLQRLVRARVPR
                     LLVTAIARDGTLEGPDLDLYRRLSHLEVPVLASGGVSSLEDLRGLARAGVEGAVVGKA
                     LYEGHFTLREAMEVPV"
     gene            complement(4709..>5208)
                     /gene="hisH"
                     /locus_tag="VF097_04820"
     CDS             complement(4709..>5208)
                     /gene="hisH"
                     /locus_tag="VF097_04820"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX6262152.1"
                     /translation="PPPADALVVPGVGAFAACVAGLRNAGLDSLVHEIVDSGRPVLGV
                     CLGMQVLFDWGEEGPADGLGILRGKVRRLPQGLTVPHMGWNEVRWGAEHLLTEGIPDG
                     TRFYFVHSYVCDPEEEITVGVTDYGGPFAAAVGRDNLFATQFHPEKSGEPGLRIYANL
                     IEAAA"
BASE COUNT          707 a         1937 c         1839 g          725 t
ORIGIN      
        1 ttcgagccca ccgagcggga cgggaggctg ttcgggcggg gggcggccga cgacaaggcc
       61 ggcgtcatgg cccacgtcgc ggccgtgctg gcccacgacg ggaagcctcc cgtgaccgtc
      121 accgccttca tcgagggcga ggaagagacg ggctcggaga acctgcccgc gttcctcgag
      181 gcccatcgtg acgcgctcca ggccgacgtc atcgtcctgg cggactcgac gaactggcgg
      241 gtcggtcagc cttccctcac cgtctcgctt cgcggcctcg tggactgcac ggtcgaggtc
      301 cggacgctcg gccacagcgt ccacagcggc ttcgtgggcg gcgtcttccc cgacgcgctc
      361 acggtgctga gccgcctgct ggccacgctc cacgacgagg agggcagcgt cgcggtcgag
      421 ggcctcctgg ccgaggaagc cgacccgctc gatctcaccg aggaggagct ccgagagcaa
      481 gccgccgcgg tccccggcct ccgggcggtg ggggagggga gcctgacctc gcggctgtgg
      541 acgaggccgg cgatctcggt gctgggcatc gacgccccga gggtgcagga ggcatcgaac
      601 acgctggtcc ccgcggcccg cgccaagatc agccttcgcc tggcgcccgg ccaggaccca
      661 caggccgcga tggaggcgct gacccggcac ctcgaggagc gggtggagtg gggcgcgcag
      721 gtggccgtga cgcccggcgc gatcgcgtac ccggcccgga tccgggcagc cgggcccgcg
      781 tacgacgccg tccgccgcgc gttccgcgag gcctggggca ccgagcccgt ggacatcggg
      841 gccggcggga cgatcccgtt cgtccaggcc ttcatggagg cgctccccga cgccgcgatc
      901 ctcgtgaccg gggtcgagga ccccgacacg cgcgcccacg ggacagacga gagcctccac
      961 ctcgaggact tcgagcgcgt gtgcgtggcc gaggccctcc tgctccggga gctcgccacc
     1021 taggggatcc gaagaacccc cgcccgggcg atgcgcggtc cgggctccct cgggtaggct
     1081 ctggttcccc cgaaggcacc ctccccccga ggagctgcgt tggcgatccg cgtggacccg
     1141 ccccgcgtcg aggcgcgcgc gagcggaagg acgatctgcc gcctctcccc gcgggacgag
     1201 gcgaggtgga gggcgctggc ggaccgcgcg gctccgctga tcgaggcccg gctccccgat
     1261 ggggtcgtgg cggaccgcgc ggtccgccag cgccacggat ggcgtctggc cccgatgcgc
     1321 ccggccctcc ggagggctcg ccgggcggcc gcgtcgctcg cccgcgaggg agcggtggtc
     1381 gccaccgacg tcgaggcctt cttcccgtcg gtcacgccgg agacgctcga gcgggccctc
     1441 ctggccgtgg gggccgcgcg gggcgacgcg gccgcggcgg catcgatgct cggcggatgg
     1501 gccgagcagg gccgcgcagg cctccccgtg ggccccgcgg cgtcctcgat cctggccaac
     1561 gccgtcctcc tccccgtgga cctcgccgtg gcgccggtcc ggttcctgcg gtgggtggac
     1621 gactacctcc tccccgcgga gtacccgctg gagcgcttcg acgaagcgct ggcggtggtg
     1681 gggctcaggc gatccgagcg gaagacggtg cgcgggatcg gcggcgtgtg gcaggccccg
     1741 tacggccgct gatcagggca tcctgcccag gcgcgtcatg agacggtcac gcttcgcctc
     1801 gagcgcggcg cgccgggccg ccagccccgc cagggcgcgc tcgaaggtcc ggacgtcctt
     1861 ctcggtctcg taggcgttcc cgcgctccac gggggtctcg tggacggcgg cgtctcgggc
     1921 cgcatcgtcc cgaaggctgc ggtgcatctc gagctcgccc tcgagcagcc gctgctcacg
     1981 atcgagggcc tgaagctcgt cgatcacctt cagcagccgt cgctccgcgc gcttcacggg
     2041 acgatcttgg agatcgggat ctcgaggatg tccgtcgctc cgtgggcctt cagcagcggg
     2101 atcagcgcgt tgaccgtcgc cttctcgacc acggtctcga tggcgaactc gtcctcctcg
     2161 gccagcgagg agacggtcgg ggccttcatg gccggcagga cctcgagcac gttgtcgagc
     2221 ttctctctgg tgacgttcag cttgatgagc acccggccct tcgcctccat cgccccctgg
     2281 atcagggtcc ggacgtcctc catcgcgcgg cgcttgtcgg ggtcctccca ggctccgtgg
     2341 tttgcgatga gcaggacctc ggactccatc agctcgtcga tgatcctcat gccgtgggcc
     2401 cggagcgtgc cgccggtctc ggagacgtcc acgatcgcgt ccacgatctc ggggaccttg
     2461 gcctcggtcg ccccgtagga ctcgaagacg cggacgtcga tgcccagctc ggcgaagcgg
     2521 cgcttggtga gctcggggta ctcggtggag atcttcgtcc cgtcggggag gtcggaggcc
     2581 ttctcccacg gcgcgtcggc ggcgaccgcg agcacgatct tcaccggccg cccggggcct
     2641 cccttggcgt aggtgagctc gaggaggatc tcgacgtcgg ccccggtctc ctcgacccag
     2701 tcccggcccg tgatccccag gtcgaagaac ccctcctcga tgtagcgggg gatctcctgg
     2761 gggcgcatca gggagacgcg gtcgatgcgc tcgtcctcga tccggccgtg gtagtcacgg
     2821 gaagagccgc ggcggacgcg caggtcggcg ccctccagga gcgagagcgt ctgctcctcg
     2881 agcgacccct tcgggatgac cagccgcagc atcctcaaac ctccaggcgc cggtagaagc
     2941 acgaccgctc gccggtgtgg cacgccggac cgtcggcgtg cacccgcacg agcagcgtgt
     3001 cggcgtcgca atcgtagcga acctcgcgga cgtgctggac gttcccgctc gtctccccct
     3061 tgcgccagag ttcaccgcgg gagcgggacc agaagaccgt gcggccctcg tcgagcgtgg
     3121 cgcggacggc ctcctcgttc atccacgcca ccatgaggac atcgcccgtg gcctcatcct
     3181 ggacgaccgc cgggatcagg cctcgctcgt cgtagtggag atcggcgacc gacgacactg
     3241 ctcgctcgct catcggcgca ccgggatccc tcgggcggcg aggtactcct tcacctgggg
     3301 cacggtgaac tcgccgaagt ggaagcggga cgcggccagc acggcgtcgg cgtgaccctc
     3361 ggtgaaggcc tgagcgaagt gctccagcgc gccggccccg ccgctggcca cgaccgggac
     3421 ctcgaccgcg ttgcccacgg cccgaagcag ttccaggtcg tagccgtccc cggtgccgtc
     3481 gcggtcgatc gacgtgagga ggacctcgcc ggcgcccaga tcggccgtgg cccgcgcggc
     3541 ccacgccacg gcgtcgaggc cggtgggcgt gcggcccccc tccacgacga cctcccagcc
     3601 gccgttccgg cgcttcgcgt cgatcgcgat cactacgcac tgcgtgccga agcggtccgc
     3661 gcagcgggcc agcagctccg gctcgcggac cccgtgcgtg ttgagcccga ccttgtcggc
     3721 cccagcccgg agcagggcgt tgacgtcctc ctcggtgcga accccgccgc ccaccgtgag
     3781 ggggatgaag atctgatcgg ccgcccgggc cacgacgtcg agcatcgccc cgcggccctc
     3841 gaccgaggcc gtgatgtcga ggaacacgag ctcgtccgcg ccctccgcgt cgtagcgggc
     3901 cgcgagctcc gcggggtcgc ccgcgtcgcg gaggtcgacg aagcgggtgc ccttcaccac
     3961 ccggcccgcc tccacgtcca gacaggggat cagcctccgg gcgagggtca caccggcacc
     4021 tccatcgcct ccctcaatgt gaagtgccct tcgtacaggg ccttccccac caccgcaccc
     4081 tcgaccccgg ctcgagccag gccccgcagg tcctccaggg agctcactcc gcccgaggcc
     4141 aggacgggga cctccaggtg gctcaacctc cggtagaggt cgagatccgg accctccagg
     4201 gttccgtcgc gggcgatcgc cgtgaccagc aggcggggga cccgggcacg gaccagacgc
     4261 tgcaggacct ggtcgaagga ccccgcgggc tcagtccagc cccggacctt gaccacgtcc
     4321 ccatcggtgt cgagggcgac gaccacgcgc tccccgtacc gctccacaca ctccgccagg
     4381 aagcccgggt ccaggatggc ctccgtgccc agcacgaccc tggccgcgcc gacggccagg
     4441 accccctcga tcagatagag gctgcgcagg ccgcccccga cctggatcgg gacgtccgcc
     4501 tcccgcgtca cgtcggcgat caccggacgg ttgtcgccga cgtcgaacgc ggcgtcgagg
     4561 tccacgacat ggacccactg cgcaccgtcc gccgcgtagc cgcgagcggt ctccacgggg
     4621 tcatcggcgt acacgatcac ctcctggagg cggccgtgtc ggaggcgcac gaccttcccc
     4681 tcacggacgt cgatcgcggg gatgacgatc acgccgccgc ctcgatcagg ttcgcgtaga
     4741 tgcggaggcc gggctcgccg gacttctccg gatggaactg ggtggcgaac aggttgtctc
     4801 gacccacggc tgcggcgaag ggccctccgt agtccgtgac ccccaccgtg atctcctctt
     4861 ccgggtcgca gacgtaggag tggacgaagt agaagcgggt gccgtcgggg atgccctcgg
     4921 tcagcagatg ctccgcgccc cagcgcacct cgttccagcc catgtggggc acagtcagcc
     4981 cttggggaag gcggcggacc ttgcccctga ggatcccgag gccgtcggcc ggtccctcct
     5041 cgccccagtc gaagaggacc tgcatcccga ggcacacgcc cagcaccgga cgccccgagt
     5101 cgacgatctc gtgcacgaga ctgtcgaggc cggcgttccg gagccccgcc acgcaggccg
     5161 cgaaggctcc cacgcccggg acgacaagag cgtcggcggg gggggggg
//
LOCUS       DASUTZ010000107         8440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_546476, whole genome shotgun sequence.
ACCESSION   DASUTZ010000107 DASUTZ010000000
VERSION     DASUTZ010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8440
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546476"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VF097_04825"
     CDS             <1..308
                     /locus_tag="VF097_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262153.1"
                     /translation="LRADADHVHRRALELFTEDEISEAFAATKGVTMPTQVRNLLRDR
                     RKQGEDILGRFRKLAKARRPISIQRWSLQRVGVLLAAILGSLIALGLLLDNLRTGAL"
     gene            305..853
                     /locus_tag="VF097_04830"
     CDS             305..853
                     /locus_tag="VF097_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262154.1"
                     /translation="MRRVLAIGALVALAGCGTPEGSIPTCPPDATDALVLVAQSVPSA
                     TQIPCVTELPVGWSYGGQVIERGRTELWLYSDRAGEQAVTVTLTPRCAFSDAVEVPSD
                     ESALTRYEEPISLTPVLSVNRYYVFEGGCVTYRFRFQEGGSYTEVLQATEALSFVSRA
                     EGAEALREAGLILCGAGVDCPG"
     gene            complement(855..1955)
                     /locus_tag="VF097_04835"
     CDS             complement(855..1955)
                     /locus_tag="VF097_04835"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HEX6262155.1"
                     /translation="MDKRSRKRALRIAEIVVSIAIVVGIFAFASPKFASYGDVWAAVQ
                     KMTWLEIATLVGISVFNIATYWPLMVASMPGLTFGQAAVNNQSSSSIANTLPGGGLIA
                     TGITYTMYRSWGFTNTQIGLTALITGIWNAFVKLAMPVMALAALAITGQGSTTLVIPA
                     LIGLAILLAAVALFAGVLWKKSLARAIGGGLGKVASLARRVVRKPSVHWGEAAVRFRK
                     QTLGLLSRRWPALTISALVSHFSLYLVLLLALRHVGVSDQEVGWAQVLGVFAFVRLIT
                     LVPLTPGGVGLVELGYIGGLYVAGRDAADVPIELFRAQITAAVLVFRTLTYALQIPMG
                     ALTYVIWQRKRSWRKPVPTEESPETAVAATQG"
     gene            complement(1979..2683)
                     /locus_tag="VF097_04840"
     CDS             complement(1979..2683)
                     /locus_tag="VF097_04840"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX6262156.1"
                     /translation="MRRTHEHPLLMNRRLDVVTAAVLLGLAAILLALMAFGETRDLLQ
                     GVDDAAARWFVSVRWAPLTGLAHLLNLLGSVWVTLPVRVAAGAHLAITRRWWHLLAFA
                     SAMFLSEAIVSILKALYDRPRPVGSLVETSGASFPSGHAVAASVTAVALVIALFPPAG
                     LHRWLWGAAAAVFAFAMALSRAYLAAHWLSDAVTGTFLGVSIALITALIVERIRGPDV
                     AEEPAPETVPRRPGTG"
     gene            2710..3663
                     /locus_tag="VF097_04845"
     CDS             2710..3663
                     /locus_tag="VF097_04845"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6262157.1"
                     /translation="MADVLRTRSKTVRPGARRAALLLILVLATACVGRPPETASTSGV
                     DLITIGSFDFSESEILAEIYAQALTLRGYRVERILRVGPRELVEPALEEGLIDLVPEY
                     LGTALEFMTHGRGTATADVNASHQALQRELSTRGIAVLEPAPAQNANGVVVTRETADR
                     YGLERISDLAPLASELVIGGPPECPERPLCLQGLGRLYGLRFREFVPTDAGGPLTAGF
                     LDAGEIDVAVLFTTSGYLGEDGYVLLEDDRGLQPAENVVPVVRGEVLRRYGAPLRDLL
                     DDISSRLEAEDLIELNRSVDLEGQHPARAASGWLTANGYAA"
     gene            3780..4061
                     /locus_tag="VF097_04850"
     CDS             3780..4061
                     /locus_tag="VF097_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262158.1"
                     /translation="MAVDERARHRLYNRVVEILGEEEAVILMAHLPPGGYPNFATKQD
                     LLDLEERLGTRFEILEHRVRAHTLRVVLTTNLVSVGVLAGVAFAATGLT"
     gene            4137..5747
                     /locus_tag="VF097_04855"
     CDS             4137..5747
                     /locus_tag="VF097_04855"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6262159.1"
                     /translation="MSRAGPHDAIVVGGGHNGLVAAAYLARRGLRTLVLERRGIVGGA
                     CVTEEIAPGVRASTTSYIASMLRPEIIRGLDLPRHGLRMVPCEPGLQVAFRDGEPLAW
                     WSDMGRTAAGIERRSAKDARAFVDLDAELKRLTRYLQPYFLEPPPNLDARGVRRMPDL
                     LRAWRRLRRLNTRDAAGLVSMLTGSLADLVHRRFESEEVRTLLLANNLYGKHGGPYEA
                     GSAVGLVFHLLSGGPEEAQGYMGHVIGGMGAITQALASAAREARAEIRTEAQVERVLV
                     RNGSATGVALADGTELEATVVLSNADPKRTFLGLVEKQELPDDFRADVEAIRMDGPCA
                     KVNLALSEEPRVVGLPADADPGRRALFTLVPSLAFAQRSADDARRGLLSDSLWVDCVV
                     ASIVDPTLAPEDTHVMTCFVQYVPYRLAEGTWDERRDELGDRVIETIARYAPNVPGSV
                     LARQVLTPLDLERTYGLTEGNIFHGDLSLDRLFFMRPVPGWSGYRTPIHGLYLCGAGA
                     HPGGGVTGAPGYNAARRVLSDLHRGGIR"
     gene            5744..6724
                     /locus_tag="VF097_04860"
     CDS             5744..6724
                     /locus_tag="VF097_04860"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX6262160.1"
                     /translation="MKGLLERLQEGVVLGDGGYLLELEKRGYVRAGPFTPEVSVTNPD
                     ALAQLHREFLRSGAEILQALTFYASEEKLATVGLAGRVDDINRAAVEVARSVAKEGDA
                     LVAGNLSLTWAYDPADPASADRVRALFDRQLEVQMEVGIDLVVAETFTWLGEALIATE
                     RALATGLPVVTTMAFENEVRAYEGDSAADCARKLRDAGAHVVGMNCLRPPEHTLPFLR
                     EIREAVDGWIAGQPVAYGTPAESPDFTSLPEFPEGLDPLQLPRGEMAAWAVEARDLGV
                     NLIGSCCGSVAAHVRAMALALGKLPEDDREWRVDYKKPMSAYEYYEHGKS"
     gene            complement(6721..7044)
                     /locus_tag="VF097_04865"
     CDS             complement(6721..7044)
                     /locus_tag="VF097_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262161.1"
                     /translation="MWVFPGERGTCERLGLGALPSGYEPAATRFAEMREEMDRRFEGA
                     ACVGEREGRAIARRVLDAHGFAAWRVRVGSGATGEGFGEMESCTSLSFDPVAREVILV
                     PETAP"
     gene            7262..>8440
                     /locus_tag="VF097_04870"
     CDS             7262..>8440
                     /locus_tag="VF097_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015801882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6262162.1"
                     /translation="MTENGFRVTYATMSADDDALHEAYERGIEDARSWLGGRHPFLVN
                     GEPREGEGWRETRSPIDRDMVIGTFAEASKQDVDDAIATAQGFAPEWAATPWEERVRL
                     LRKAADLISERRSELSALMAIEVGKNRLEALGDVEETADLIRYYCHEIERNDGFAKPM
                     GQLSEAEKTRDVMRPYGVWGVISPFNFPRALAGGPSGAALVAGNTVVLKPSGQGYFTG
                     LKLGEALLDAGIPQAAFQVLPGKGSVAGAALVADPRLGGITFTGSYEVGMGIYKSFTQ
                     DYPRPVICEMGGKNPVIVTAKADLEKAAEGIKRSAFGFGGQKCSAASRLYVEAPVRDE
                     LVSALLEKTSEIRIGDPLERDVWLGPVIDEKAVARYEEAVAEARANGEVLHGGERLTE
                     G"
BASE COUNT         1293 a         2913 c         3093 g         1141 t
ORIGIN      
        1 cgctccgggc cgacgccgac cacgtccacc ggcgtgcgct ggagctcttc acggaggacg
       61 agatctccga ggccttcgcc gccacgaagg gcgtgacgat gcccacccag gtccggaacc
      121 tgctgcgcga ccggcgcaag cagggggagg acatcctggg ccggttccgg aagctggcca
      181 aggcgaggcg acccatctcg atccagcgat ggagcctcca gcgggtgggg gtcctgctgg
      241 cggccatcct ggggtcgctg atcgcgctgg gtctcctcct cgacaacctg cggacggggg
      301 cgctgtgagg cgcgtcctcg cgatcggcgc gctggtggcc ctggctggct gtgggacgcc
      361 ggaggggtcg atcccgacct gtccgcccga cgcgacggac gcgctcgtcc tggtcgcgca
      421 gtcggtgccc tccgccaccc agatcccgtg cgtcacagag ctgccggtgg ggtggagcta
      481 cggggggcag gtcatcgagc gggggcgaac cgagctctgg ctctactcgg atcgcgcggg
      541 cgaacaggcg gtcaccgtca cgctcacccc acggtgcgcc ttctccgacg cggtggaggt
      601 ccccagcgac gagtccgcgc tgacccgcta cgaggagccg atctcgctga cccccgtcct
      661 cagcgtgaac cgctactacg tcttcgaggg cgggtgcgtt acctatcggt tccggttcca
      721 ggagggcggc tcgtacacgg aggtgctcca ggccacggag gcgctcagct tcgtgtcccg
      781 agcggaaggg gcggaggcgc tgcgcgaggc cgggctgatc ctgtgcggtg cgggggtgga
      841 ctgcccggga tagatcagcc ttgggtcgcc gccaccgccg tctcggggga ctcctccgtg
      901 gggacgggct tccgccagct ccgcttgcgc tgccagatga cgtaggtcag cgctcccatc
      961 gggatctgga gcgcgtaggt gagggttcgg aacacgagca cggcggcggt gatctgggcg
     1021 cggaacagct cgatggggac gtcggccgcg tcccgcccgg ccacgtacag gcctccgatg
     1081 tagcccagct cgacgaggcc gacgccaccg ggcgtgagcg gcaccagcgt gatcaggcgg
     1141 acgaacgcga acacgcccag cacctgcgcc cagccgacct cctgatcgga cacgcccacg
     1201 tgccggagcg ccagcagcag cacgaggtac agcgagaagt ggctgaccag cgcggagatg
     1261 gtgagggcgg gccaccgcct cgagagcagc ccgagggtct gcttgcggaa gcggaccgcg
     1321 gcctcgcccc agtgaaccga gggctttcgc accacccttc gcgccagcga ggccaccttc
     1381 cccaggccgc cgccgatcgc acgggccagc gacttcttcc acagcacccc ggcgaagagc
     1441 gcgacggccg ccagcaggat ggcgagaccg atcagggccg ggatgacgag ggtcgtgctc
     1501 ccctgccccg tgatggccag ggccgccagg gccatcacgg gcatggccag cttcacgaac
     1561 gcgttccaga tcccggtgat gagcgcggtg aggccgattt gcgtgttcgt gaagccccac
     1621 gagcggtaca tcgtgtaggt gatccccgtc gcgatgagcc cccctcccgg cagggtgttc
     1681 gcgatcgagc tcgaggactg gttgttgacg gcggcctgac cgaaggtcag gccgggcatc
     1741 gaggccacca tcaacggcca gtaggtggcg atgttgaaca ccgagatgcc gacgagcgtg
     1801 gcgatctcca gccaggtcat cttctggacc gcggcccaga cgtccccgta gctggcgaac
     1861 ttggggctgg cgaaggcgaa gatccccacc acgatggcga tggagacgac gatctccgcg
     1921 atccggagcg cgcgcttccg ggatctcttg tccacgcgtc ggacctcccg gacgaagcct
     1981 agcccgtccc ggggcgtcga gggacggtct ccggagcggg ctcctccgcc acgtcgggac
     2041 cccggatccg ctccacgatc aaggcggtga tgagggcgat gctcacgccg aggaaggtcc
     2101 cggtcaccgc gtcggagagc cagtgggcgg cgaggtacgc gcgcgacagg gccatggcga
     2161 aggcgaacac cgcggcggcg gccccccaca gccaccgatg gagcccggcg ggagggaaga
     2221 gcgcgatgac cagggccacc gccgtcaccg aggccgccac cgcgtgaccg gaggggaacg
     2281 aggccccgct cgtctcgacc agggagccga ccggccgcgg ccgatcgtag agggccttca
     2341 ggatcgagac gatggcctcc gagaggaaca tggccgaggc gaacgcgagc aggtgccacc
     2401 accggcgcgt gatcgcaagg tgggcgcccg ccgcgacccg gacgggcagc gtgacccaca
     2461 cggaccccag caggttcagg aggtgagcca ggcccgtcag cggggcccac cgaacggaga
     2521 cgaaccaccg ggcggcggca tcgtcgacgc cctgaaggag atccctcgtc tccccgaagg
     2581 ccatcagggc caagaggatc gccgctaggc cgaggaggac cgcggccgtc acgacgtcca
     2641 gccggcggtt catcaggagc ggatgctcgt gggttctgcg catgcggaac aagagtagtg
     2701 ttcgaaaccg tggcggacgt cctcaggacg cgatcgaaga ccgtccggcc gggggcgcgc
     2761 cgggccgcgc tcctcctgat cctggtcctg gccacggcgt gcgtgggccg gccccccgag
     2821 accgcgagca cctcgggcgt ggacctgatc acgatcggct ccttcgactt ctccgagagc
     2881 gagatcctgg cagagatcta cgcccaggcg ctcacgctgc gcggctatcg ggtcgagcgg
     2941 atcctccggg tcggtccgcg agagctggtg gagccggcgc tggaggaggg gctgatcgac
     3001 ctcgtccccg agtacctggg gacggcgctg gagttcatga cgcacggacg cgggacggcg
     3061 accgcggacg tgaatgcctc ccaccaagcg ctccagcggg agctctcgac gcgggggatc
     3121 gcggtgctgg agccggcccc cgcccagaac gcgaacgggg tcgtggtgac ccgggagacg
     3181 gcagacaggt acgggctcga gaggatcagc gacctggccc ccctcgcgag cgagctggtg
     3241 atcggcgggc ccccggagtg cccggagcgt cccctgtgcc tccagggcct cgggcgtctg
     3301 tacggcctca ggttccggga gttcgtcccc accgacgccg gcggcccgct gacggccggg
     3361 ttcttggacg ccggagagat cgacgtcgcc gtgctgttca ccacgagcgg atacctgggc
     3421 gaggacggat acgtcctcct ggaggacgac cggggcctcc agcccgccga gaacgtcgtc
     3481 ccggtcgtgc ggggggaggt cctccgccgc tacggcgcgc cgctgcggga cctcctcgac
     3541 gacatctcaa gccggctcga ggccgaggat ctcatcgagc tgaaccgttc ggtcgacctc
     3601 gagggacagc acccggcccg cgccgcgtcg gggtggctca cggccaacgg ctacgcggcc
     3661 tgaaccctcc tcgatgtgcc cattccaact tctcttaagg gccggcacgc ccccccgttg
     3721 catgtcagag gtgggtcata cgatcggagc actccccctc acgacaccag ggggggataa
     3781 tggccgtcga tgagcgagcc aggcatcggc tgtacaaccg agtcgtggag atcctgggcg
     3841 aggaggaggc cgtcatcttg atggcgcatc tgccgccggg cggctacccg aacttcgcca
     3901 ccaaacagga cctcctcgac ctcgaggagc gactcggaac gcggttcgag atcctcgagc
     3961 atcgcgttcg agcgcacacc cttcgcgtgg tcctgacgac gaatctcgtc tcggttgggg
     4021 tcctcgcggg ggtggcgttc gctgccacag gcctcacata gccgggcgtc gcgctctccg
     4081 acttccaccg gtatagtccc tgggccgacc tgagcgggcc ggcgtctggg ggacgtatgt
     4141 cgcgagcggg gccccacgac gcgatcgtcg tgggcggggg acacaacggc ctggtcgccg
     4201 ccgcctacct cgctcgtcgg ggcctgcgca ccctcgtcct ggagcggcgc gggatcgtgg
     4261 gaggggcctg cgtcacggag gagatcgccc cgggcgtccg cgcctccacc acctcgtaca
     4321 tcgccagcat gctgcggccc gagatcatcc gtgggctcga cctgccgcgg cacggcctcc
     4381 ggatggtgcc gtgcgagccg ggcctccaag tggcgttccg ggatggcgag ccgctcgcct
     4441 ggtggagcga catggggagg accgcggcgg ggatcgaacg gcgctcggcg aaggacgcgc
     4501 gggccttcgt ggacctcgac gcggagctga agcgcctgac gcggtacctc cagccctact
     4561 tcctggagcc gcctcccaac ctcgacgcgc gcggcgtccg caggatgccc gacctcctcc
     4621 gtgcctggcg gcgcctccgg aggctgaaca cccgcgacgc ggcggggctc gtctcgatgc
     4681 tgacgggctc gctggcggac ctcgtgcatc ggcgcttcga atccgaggag gtccgcaccc
     4741 tgctgctcgc gaacaacctc tacgggaagc acggcgggcc ctacgaggcg gggtcggcgg
     4801 tcggcctggt cttccacctc ctctccggcg ggcccgagga ggcgcagggg tacatggggc
     4861 acgtgatcgg agggatgggg gcgatcacgc aggcgctggc ctcggcggcg cgggaggcga
     4921 gggccgagat ccggaccgag gcccaggtcg agcgggtgct cgtgcggaac ggctcggcga
     4981 ccggggtggc gctggccgac ggcacggagc tcgaggcgac ggtcgtgctg tcgaacgcgg
     5041 acccgaagcg cacgttcctc gggctcgtgg agaagcagga gctgcccgac gacttccggg
     5101 ccgacgtcga ggcgatccgc atggacggcc cctgcgccaa ggtcaacctc gcgctgagcg
     5161 aggagccgcg cgtcgtcggc ctcccggccg acgcggaccc cggccgccgc gccctgttca
     5221 ccctggtgcc gtcgctggcc ttcgcgcagc ggtcggccga cgacgcgcgg cgaggcctcc
     5281 tgagcgactc cctctgggtc gactgcgtgg tggcctcgat cgtggacccc accctcgccc
     5341 ccgaggacac ccacgtcatg acgtgcttcg tccagtacgt gccctaccgc ctggcggagg
     5401 gcacctggga cgagcggcgg gacgagctcg gcgaccgggt gatcgagacg atcgcgcggt
     5461 acgcgcccaa cgtgccgggc tcggtgctgg cccgccaggt cctcacgccc ctcgacctgg
     5521 agcgcaccta cggcctcacc gagggcaaca tcttccacgg cgacctgagc ctcgaccgcc
     5581 tcttcttcat gcggccggtg cccggctgga gcggctaccg caccccgatc cacgggctgt
     5641 acctgtgcgg cgcgggggcg cacccgggcg gcggcgtgac cggcgcgccc ggctacaacg
     5701 cggcccgccg ggtcctgagc gacctgcatc gaggagggat ccgatgaagg gcctgctgga
     5761 gcggctccag gagggcgtgg tgctcggcga cggcggctac ctgctcgagc tggagaagcg
     5821 cgggtacgtg cgggcgggcc ccttcacgcc agaggtcagc gtcacgaacc ccgacgcgct
     5881 ggcccagctc caccgggagt tcctgcggag cggggccgag atcctccagg ccctgacgtt
     5941 ctacgccagc gaggagaagc tggccacggt cggtctcgcg ggccgcgtgg acgacatcaa
     6001 ccgggcagcg gtggaggtgg cgcggtcggt ggccaaggag ggcgacgcgc tggtggcggg
     6061 caatctcagc ctgacctggg cctacgaccc ggccgatcct gcctcggccg accgcgtccg
     6121 tgcgctcttc gaccgtcagc tcgaggttca gatggaggtg gggatcgatc tcgtcgtggc
     6181 cgagacattc acctggctgg gcgaggctct gatcgccacc gagcgtgcgc tggccacggg
     6241 gctcccggtg gtgacgacga tggcgttcga gaacgaggtg cgggcgtacg agggggactc
     6301 ggccgccgac tgcgcccgca agctccgcga cgccggcgcg cacgtggtgg gcatgaactg
     6361 cctgcggccg cccgagcaca cgctcccgtt cctccgcgag atacgcgagg cggtggacgg
     6421 atggatcgcg ggccagccgg tcgcgtacgg gacgccggcg gagagccccg acttcacctc
     6481 gctccccgag ttccccgagg ggctggaccc tctccagctg ccacgggggg agatggcggc
     6541 gtgggcggtg gaggcgaggg acctcggcgt gaacctgatc ggctcgtgct gcggctcggt
     6601 ggccgcgcac gtgcgggcga tggccctcgc cctgggcaag ctccccgagg acgatcgcga
     6661 gtggcgggtc gactacaaga agccgatgtc cgcctacgag tactacgaac acggcaaaag
     6721 ctagggagcc gtctctggga ccaggatgac ctccctcgcg accgggtcga aggaaagcga
     6781 ggtgcacgac tccatctcgc cgaagccctc gccggtcgct cccgagccca cccgcacgcg
     6841 ccacgctgcg aacccgtgag cgtcaagcac ccgacgggcg atcgcgcgac cctcacgttc
     6901 gccgacgcat gccgccccct cgaaccggcg atccatctcc tcacgcatct ctgcgaaccg
     6961 tgtagccgcg ggctcgtagc ccgacggcag agcgcccagg cccagccgct cacaggtgcc
     7021 ccgctctccg gggaacaccc acaccgcctc ccgaacgcct cgaccacgcc gtctcgggtt
     7081 aggcggagga gctcgcgatc cgtcggatcc gtcatctgac cctttcgtgt cctcgacgga
     7141 gcgtcggttc ccccgagtat cgcagtccgg tgcgaaggga gcgcgagggc tagactctgc
     7201 ccaccgacga gccgccgacg aggaccggcg gatcattgtg tctcgcagag gagggacggc
     7261 gatgaccgag aacgggttcc gggtgacgta cgcgacgatg tcggcggatg acgacgcgct
     7321 ccacgaggcg tacgagcgcg ggatcgagga cgcgaggtcg tggctgggcg gccggcatcc
     7381 cttcctcgtg aacggcgagc cgcgagaggg tgaggggtgg cgagagaccc gctccccgat
     7441 cgaccgcgac atggtgatcg ggaccttcgc cgaggcctcc aagcaggacg tggacgacgc
     7501 gatcgccacc gcgcagggct tcgcccccga gtgggcggcc accccgtggg aggagcgggt
     7561 ccggctcctg cggaaggccg ccgacctcat ctcagagcgg cgcagcgagc tctccgccct
     7621 catggcgatc gaggtgggga agaaccgcct cgaggccctc ggcgacgtcg aggagaccgc
     7681 cgacctgatc cgctactact gccacgagat agagcggaac gacgggttcg ccaagccgat
     7741 ggggcagctc tccgaggccg agaagacgcg cgacgtcatg cggccctacg gcgtctgggg
     7801 cgtgatcagc ccgttcaact tcccgagggc gctggccggc ggcccgagcg gcgccgccct
     7861 cgtcgcgggc aacaccgtcg tgctgaagcc gtccggccag ggctacttca ccgggctgaa
     7921 gctcggcgag gcgctgctcg atgccggcat cccgcaggcc gccttccagg tgctgcccgg
     7981 gaaggggagc gtggccgggg ccgccctggt cgccgacccg cggctgggcg gcatcacctt
     8041 caccgggtcg tacgaggtcg ggatgggcat ctacaagtcg ttcacccagg actacccgcg
     8101 tcccgtgatc tgcgagatgg gcgggaagaa cccggtgatc gtgacggcca aggccgacct
     8161 ggagaaggcc gccgagggga tcaagcgctc ggcgttcggg ttcggggggc agaagtgctc
     8221 ggcggcctca cgcctctacg tcgaggcgcc ggttcgcgac gagctcgtga gcgcgctgct
     8281 ggagaagacc tccgagatcc ggatcggcga tcccctggag cgagacgtct ggctggggcc
     8341 cgtgatcgac gagaaggcgg tggccaggta cgaggaggcg gtggcggagg cgagggcgaa
     8401 cggcgaggtc ctccacggcg gcgagcggct cacggagggc
//
LOCUS       DASUTZ010000108         8645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1194304, whole genome shotgun sequence.
ACCESSION   DASUTZ010000108 DASUTZ010000000
VERSION     DASUTZ010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8645
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194304"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..535)
                     /locus_tag="VF097_04875"
     CDS             complement(41..535)
                     /locus_tag="VF097_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX6262163.1"
                     /translation="MRPILPMLLAALLVPAIAMAEPAGQTFTAHLSGGEEVQANDSIA
                     AGRAIFHVWKDGTSIDYKLIVANIENVTQSHIHLAPAGANGGVVAWLYPSAPPAQLIP
                     GRTDGILATGTITGANLVGALAGQPLSALIEHMRNGNAYVNAHTSQFPGGEIRGQIAA
                     RGPQ"
     gene            complement(601..1389)
                     /locus_tag="VF097_04880"
     CDS             complement(601..1389)
                     /locus_tag="VF097_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017251574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX6262164.1"
                     /translation="MEQITYERDGGVGWISLNRPDKLNALTSVMSDELCDVLEELRDD
                     DEVRAVVLTGAGRGFCAGQDLTEFRDTGDAGDRLDIEQHLAETYHRLIPLMVESPKPV
                     IAGVNGVAAGAGLSLAIACDVRIAGEEARFTQAFVKIGLIPDSGGTYLLPRIVGYAKA
                     LELSMSGEMIDAAEAHRIGLVSRVTPAADLEEEVRAYAAALAAMPTLAIAEAKRLLRD
                     ALTLDLREALHREAKAQARMGATEDFREGVTAFVEKREPSFRGR"
     gene            complement(1393..1773)
                     /locus_tag="VF097_04885"
     CDS             complement(1393..1773)
                     /locus_tag="VF097_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HEX6262165.1"
                     /translation="MQRERPAPGDTATIEVIVTEDMLVDLGGRRIHPLYATASMVRHV
                     EEAGRMLVEPHFRDGEDATGFAVSIVHERPARVGERLTVLARATRVDEWECEAEVEVR
                     GPAGVVGRGTLVQRYIEAGRFDGG"
     regulatory      1825..1937
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1926..3374
                     /locus_tag="VF097_04890"
     CDS             1926..3374
                     /locus_tag="VF097_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEX6262166.1"
                     /translation="MVSAGAALGITLAALALFAAIGVAEARRLGHADRDRYLTARGTQ
                     GAGALALSFFASALGTWILFAPPEVGTFGGLLGILGYAVGQAAAIAIFAWLGPMVRDR
                     LPSGSTILGWVRERFGPAASAVVVVVSVGYMFIFLTAELTAIGGVLGLLAGVDPLVPV
                     VAVALATAAYTAYGGLPASVATDRWQGWLVLAFLAIAAGAVVAEIGSPVERLRVGGAG
                     TVTRLGAETFVTLFIAIVAANLFHQGFWQRVWAARDRDGLVRGAIGGATLILPVVLVT
                     GLLGAMAAGAGRAEAPSLAFFALLEGLPEPVLWIAVALAVALVASTTDTLQNGLASLA
                     ATEVGGRSISLGAARWLTVALTVPGAAIAVQGYSVLRLFLIADLLAAAIAAPVFLGLW
                     RRATSTGVLAGAAAGIAGVVAAGWIATGDLPEGFRLLTLPPDRLDLRLGAFAAAAAAS
                     TAVALLVAVLSPRAEAPSPSAPRADPPTRSSR"
     gene            complement(3304..3627)
                     /locus_tag="VF097_04895"
     CDS             complement(3304..3627)
                     /locus_tag="VF097_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262167.1"
                     /translation="MEQANLTDHVHFEESEVARSALFESERLFSQLLCLSRNQSYGPA
                     SDAGSDAMLTVLAGEAAFQIGKRRARLKQWGAVLVPAGADVTVKNASADPLVVLMVTA
                     PPPAG"
     gene            3705..5183
                     /locus_tag="VF097_04900"
     CDS             3705..5183
                     /locus_tag="VF097_04900"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX6262168.1"
                     /translation="MPRIDYAATPPAKHDADLLVLPVFEGPEEGPGLKEAGRAMGEDL
                     LELLRSNGFRGKRGDAFSVPTFGRLPAGTLLLVGLGKRDEVDADRVRRVIGKLAPRLH
                     GYRSVATTLPQVARGGWEEAVQAAAEGLVLGSYRFLRYKKEEEERKLRRLTLLGTGRW
                     DEGRAERAAGRGEVVAEAACWARDLVNTPAIDATPEFLAAEARKMARETGLEVKVWTK
                     RRLEEGGFGGILGVGSGSANDPRLIELTYSGGRGKPIALAGKGITFDSGGLSLKDAKQ
                     MEWMKADMSGAAAVMATMRAIAELKPKVNVVAAIPSAENMPGGAAIRPGDVLRHRGGK
                     TTEVLNTDAEGRLILADALAYLAERKPRLIVDTATLTGACMIALGEQLYGVMSNDDGL
                     ARELIAAGGKAGEPGWQLPLWAGYREQVSSTVADVKNIGSRYGGAINAALYLSEFVGE
                     IPWAHLDIAGVAYAQNGGDYGKGATGIPVRTLVRFVLDRAGG"
     gene            complement(5185..6051)
                     /locus_tag="VF097_04905"
     CDS             complement(5185..6051)
                     /locus_tag="VF097_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6262169.1"
                     /translation="MFRRRRPGVAGATPQRTPSGYHQEDWESYDHVAEAYERVTSERT
                     SRAASDLVELAGVGPGHRVLDVGAGTGVAAIAAIEHVGAGGLVVGLDPSSKMLALARP
                     KHPEVHYVQGEALDLPFRDASFDRVIGNFVVLHFNRWETALFDMVRVLRPGGVMALST
                     WAASEDAFLEAWRQVAEELVGPELYRDASRRAAPWQERFSNPALFEESLKQAGLRRIR
                     IERRSYRFEMTLESYLEGMETRTLARSLHRIMGDAIWERFRERVRGEFDDRFAERIGD
                     TRDVWFGLGVKE"
     gene            6103..7740
                     /locus_tag="VF097_04910"
     CDS             6103..7740
                     /locus_tag="VF097_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6262170.1"
                     /translation="MNGPRHGGHLLAALLRQEGVEHLFTLSGGHIAPIYDGCVAEGIR
                     IVDFRHEQAAAHAADGWARVTGRPGVAAVTAGPGVTDAVTGVANAFFADSPMVLIGGR
                     APLAEQRMGSLQEMDHVALMRSITKWADTCWQTRRIADYAAEALRHATTGRQGPVFLD
                     VPLDVQFDVWPTDVELPRGFRPEGRPQGDPDLVKRAAKMLADAERPVVFAGSGVRWSE
                     AHEALAALADALKIPVFLNSLGRGSLPPEHRSFFSAARKHALGKADLVLALGVDWDFR
                     LGFGRRNLPESVRVIQVDVDGRHIGRNRPVDVGIVGDPGRVIEQLLAEGVGSPQEPSW
                     TEEVRDVERAKHEEARAGMDSDAVPIHPQRFAREIRDFLDEDATVVGDGGDIVGISSS
                     IVQARRPGHWLDPGPFGCLGVGPSFAIAAKLARPDTQVAVIYGDGSFGLNAMEYESAI
                     RQDIPFVGIIGNDGAWGQIKVAQEALYGEGNAPAATLSTEVGYERMVEGLGGYGERVT
                     DPSQLGPALRRAFDSGVAACLNVIVDPSLMRRASYLG"
     gene            7796..8608
                     /locus_tag="VF097_04915"
     CDS             7796..8608
                     /locus_tag="VF097_04915"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012692311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEX6262171.1"
                     /translation="MRKLIPMLSVVLVLALASPGGAIVNGELDGDDHPNVGALIGDFD
                     GDKIIICSGTLIAPDVFLTASHCTAFLESIGQSTAWVSFDSELEEPVTSPPATLHQGT
                     LHTHPDYGFSGPGGVSDTHDIAVVVLDQPVQGIQPASLPTLNQLGQAAAKNGLKRQKF
                     TAVGYGVHEAVLGGGPPSFPFDGARWRAVSEYRSLKNAWLHLSQNPSTGDGGTCFGDS
                     GGPNFLGAGAGETDVVAGITVMGDSMCRATNVTYRLDTASARNFLDDFVALP"
BASE COUNT         1160 a         3037 c         3183 g         1265 t
ORIGIN      
        1 cccgccgctc cgcggcgggc gcctctcacg caggatcgag ctactggggg ccgcgcgcgg
       61 cgatctgtcc ccggatctcg ccgccgggga actggctcgt gtgggcgttg acgtacgcgt
      121 ttccgttcct catgtgctcg atgagcgccg acagcggctg acctgcgagc gctcccacca
      181 ggttggcccc ggtgatcgtg cccgtggcca ggatcccgtc ggtccggccg gggatcagct
      241 gcgccggcgg cgccgagggg tacagccagg ccaccacgcc gccgttggcg cccgccggcg
      301 ccaggtggat gtgcgactgc gtcacgttct cgatgttggc cacgatcagc ttgtagtcga
      361 tcgaggtgcc gtccttccag acgtggaaga tcgcccggcc cgcggcgatc gagtcgttgg
      421 cctgcacctc ctcgccgccg gagaggtgag cggtgaaggt ctgaccggcg ggctccgcca
      481 tggcgatcgc cgggacgagc agggccgcga gcagcatcgg gaggatgggt cgcatgatct
      541 cttcttacgg gttgggcgtt ccgcgaccgt atcgcgaggt tcgagaaacg gcaacgcgag
      601 ctagcggccc ctgaagctcg gctcgcgctt ctcgacgaag gccgtgacgc cctcccggaa
      661 gtcctcggtc gcacccatcc gcgcctgggc cttcgcctcc cggtgcagcg cctcgcggag
      721 gtccagggtg agcgcgtccc gaaggagccg cttcgcctcc gcgatggcca acgtgggcat
      781 cgcggccagg gcagcggcgt acgcgcgcac ttcctcctcg agatccgccg cgggcgtgac
      841 gcggctcacc aggccgatcc ggtgggcctc ggccgcgtcg atcatctcgc cgctcatcga
      901 gagctcgagc gccttggcgt acccgacgat ccggggaagc aggtaggtgc cgcccgagtc
      961 ggggatcagc ccgatcttca cgaaggcctg cgtgaacctc gcctcctcgc cggcgatgcg
     1021 gacgtcgcag gcgatggcca gcgagagccc ggccccggcc gcgacgccgt tcacgccggc
     1081 gatcacgggc ttcggcgact ccaccatgag cgggatgaga cggtggtagg tctcggcgag
     1141 atgctgctcg atgtcgagac gatctccggc gtcgccggtg tcgcggaact ccgtgaggtc
     1201 ctgtccggcg cagaagcctc ttcccgcgcc ggtcagcacg accgcgcgca cctcgtcgtc
     1261 gtcgcggagc tcctcgagga cgtcgcacag ctcgtcgctc atcaccgagg tgagggcgtt
     1321 cagcttgtcc gggcggttca gcgagatcca gccgacgccg ccgtcccgct cgtaggtgat
     1381 ctgctccatc gctcagcctc cgtcgaatcg tcccgcttcg atgtagcgct gcacgagcgt
     1441 gccccggccg accacgccgg ccggcccgcg gacctcgacc tcggcctcgc actcccactc
     1501 atccaccctg gtggcgcggg ccagcaccgt gagccgctct cccacccggg ccgggcgctc
     1561 gtgcacgatc gagaccgcga agcccgtggc gtcctcgccg tcgcggaagt gcggctcgac
     1621 gagcatccgg cccgcctcct ccacgtggcg caccatcgag gccgtggcgt agagggggtg
     1681 gatccttcgg cctccgagat ccaccagcat gtcctcggtg acgatcacct cgatcgtggc
     1741 cgtatcgccc ggcgccggcc gctcccgctg cacgccgctc atcctagtcg gcggctagcg
     1801 ggctctgccg ggcggacggt aggctctgcg gagcagggga gctcggaagg gaccgggctg
     1861 agaggccggc tggggagccg gcgaccctcg aacctgatcc gggtaacgcc ggcggaggga
     1921 gcgacatggt gagcgcaggc gcggcgctgg ggatcaccct cgcggcgctg gcgctgttcg
     1981 cggcgatcgg tgtggccgag gcgaggcgcc tcggccacgc cgatcgcgac cgctacctca
     2041 ccgctcgcgg gactcagggc gccggcgccc tcgcactgag cttcttcgcc tcggcgctgg
     2101 gcacgtggat cctcttcgcc ccgcccgagg tcggaacctt cggaggcctc ctcgggatcc
     2161 tgggctacgc ggtggggcag gccgccgcca tcgcgatctt cgcgtggctg gggcccatgg
     2221 tgcgcgaccg gctcccctcg ggctccacga tcctgggatg ggtgagggag cggttcggcc
     2281 ccgcggcgag cgcggtggtc gtggtcgtct cggtcggcta catgttcatc ttcctcacag
     2341 cggagctcac cgcgatcggc ggcgtgttgg gtctgctggc gggtgtggac cccctcgtgc
     2401 ccgtggtcgc ggtggccctg gccacggccg cctacacggc ctacggcggg ctgccggcct
     2461 cggtggccac cgaccggtgg caggggtggc tcgtgctggc cttcctggcg atcgcggccg
     2521 gcgccgtggt ggctgagatc gggtcgccgg tcgagcggct ccgcgtcggc ggcgcgggga
     2581 cggtgacgcg gctgggggcc gagaccttcg tcaccctctt catcgcgatc gtggctgcga
     2641 acctcttcca ccaggggttc tggcagaggg tgtgggccgc gcgcgaccgg gatgggctcg
     2701 tccgaggggc gatcgggggc gccacgctga tcctcccggt ggttctggtg accggcctcc
     2761 tgggggcgat ggcggcgggg gccggacgtg ccgaggcgcc ctcgctggcg ttcttcgcgc
     2821 tgctggaggg gctccccgag cccgtgctct ggatcgcggt ggcgctcgcg gtcgcgctcg
     2881 tggcctccac gaccgacacg ctgcagaacg ggctggcgtc gctggcggcg accgaggtgg
     2941 gcggcaggag catctccctc ggagccgcca ggtggctcac ggtggcgctg acggtgccgg
     3001 gggcggcgat cgccgtgcag ggctacagcg tgctgcgcct gttcctgatc gccgaccttc
     3061 tggccgcggc gatcgccgcc cccgtcttcc tcggcctgtg gcggagggcg acctcgacgg
     3121 gagtgctggc gggagcggct gcggggatcg cgggcgtggt cgccgcgggc tggatcgcga
     3181 ccggtgacct cccggagggg ttccgcctcc tcaccctccc ccccgaccgg ctggacctcc
     3241 gcctgggggc cttcgcggcg gcggccgccg cctcgacggc cgtggccctg ctcgtcgcgg
     3301 tcctcagccc gcgggcggag gcgccgtcac catcagcacc acgagcggat ccgccgacgc
     3361 gttcttcacg gtgacgtccg ccccggccgg gaccaggacc gctccccact gcttcaggcg
     3421 cgcccgacgc ttcccgatct ggaacgcggc ttcgccggcg agcacggtga gcatcgcgtc
     3481 ggagcccgcg tcgctcgcgg gcccgtacga ctggttccgc gagaggcaca gcagctggct
     3541 gaagagccgc tccgactcga agagcgcgct ccgcgcgacc tccgactcct cgaagtggac
     3601 gtggtcggtg agattcgcct gctccatcac ggtccagcgt acgggcccgg acgacccccg
     3661 gcaaagcgtc tagactgcgc gtctgcagac tccaggaggt tcccttgccg aggatcgact
     3721 acgccgcgac cccgcccgcc aagcacgacg ccgacctgct cgtcctcccc gtcttcgagg
     3781 ggcccgagga ggggccgggc ctgaaggagg cgggtcgagc catgggcgag gacctcctcg
     3841 agctcctgcg ctcgaacggg ttccggggga agcgggggga cgcgttctcg gtccccacgt
     3901 tcgggcgcct gcccgccggc accctgctgc tcgtcgggct cgggaagcgc gacgaggtcg
     3961 atgccgaccg cgtccgccgc gtgatcggga agctcgctcc acgtctccac ggctaccgga
     4021 gcgtggccac gaccctgccc caggtcgctc ggggcggctg ggaggaagcg gtccaggccg
     4081 ccgccgaggg gctcgtcctc ggaagctacc gcttcctccg gtacaagaag gaagaggagg
     4141 agcggaagct gcggcgcctg accctcctcg gcacgggccg ctgggacgag ggccgcgccg
     4201 agcgcgccgc agggcgcggc gaggtcgtgg ccgaggcggc ctgctgggcc cgggatctcg
     4261 tgaacacgcc cgcgatcgac gccacgcccg agttcctggc cgccgaggcc cggaagatgg
     4321 cgcgggagac cggcctcgag gtcaaggtct ggaccaagcg gcgcctcgag gaggggggct
     4381 tcggcgggat cctcggcgtg ggctcgggca gcgccaacga cccacggctg atcgagctca
     4441 cctactcggg gggccggggg aagcccatcg ccctggcggg gaagggcatc acgttcgact
     4501 ccggaggcct ttcgctgaag gacgccaagc agatggagtg gatgaaggcg gacatgtccg
     4561 gggccgccgc ggtcatggcc acgatgcggg cgatcgccga actgaagccg aaggtgaacg
     4621 tcgtggcggc catccccagc gccgagaaca tgccaggagg cgccgcgatc cgtcccggcg
     4681 acgtcctccg tcaccgcgga ggcaagacga ccgaggtcct caacaccgac gccgagggac
     4741 ggctgatcct ggcggacgcg ctggcctacc tagcggagcg gaagcctcgt ctgatcgtgg
     4801 atacggccac gcttaccggt gcctgcatga tcgctctcgg cgagcagctc tacggcgtca
     4861 tgagcaacga cgacggcctg gcccgggagc tcatcgcggc gggggggaag gccggggagc
     4921 cgggctggca gctcccgctc tgggctggct atcgggagca ggtctcctcg accgtggcgg
     4981 acgtgaagaa catcgggagc cgctacggtg gcgcgatcaa cgcggcgctg tacctctcgg
     5041 agttcgtggg cgagatcccg tgggcgcacc tcgacatcgc cggcgtggcc tacgcacaga
     5101 acggcggtga ctacgggaag ggcgccacgg gcatcccggt gaggacgctg gtgcgcttcg
     5161 tgctcgaccg ggccggcggc tgagctactc cttcacgccg aggccgaacc agacgtcgcg
     5221 ggtgtcgccg atccgctcgg cgaagcgatc gtcgaactcg cctcgcaccc gctcccggaa
     5281 ccgctcccag atcgcatccc ccatgatccg gtggagcgag cgagccagcg tccgcgtctc
     5341 catgccctcg aggtaggact cgagcgtcat ctcgaagcgg taggagcgcc gctcgatcct
     5401 gatccgccgg aggcccgcct gcttcagcga ctcctcgaag agggccgggt tcgagaagcg
     5461 ctcctgccac ggcgccgcgc ggcgggaggc gtcgcgatag agctcgggac cgacgagctc
     5521 ctcggccacc tgacgccagg cctcgaggaa ggcgtcctcg ctcgccgccc aggtcgagag
     5581 ggccatcacg ccacccggac ggaggacccg gaccatgtcg aagagggcgg tctcccatcg
     5641 attgaagtgg aggacgacga agttcccgat cacccggtcg aacgaggcgt cgcggaaggg
     5701 gaggtcgagg gcctcgccct gcacgtagtg gacctcgggg tgcttcgggc gggccagcgc
     5761 cagcatcttg gaggagggat cgagccccac gacgaggccg cccgcgccca cgtgctcgat
     5821 cgcggcgatc gcggcgaccc cggtgccggc gcccacgtcg aggacccgat ggcccggacc
     5881 caccccggcc agctcgacga gatcgctggc ggcgcgggac gtgcgctccg atgtgacccg
     5941 ctcgtaggcc tcggctacgt ggtcgtagga ctcccagtcc tcttggtggt agccggaggg
     6001 cgtgcgctgg ggggtggctc ccgcaacccc cggcctccgt ctccggaaca tgccgcctat
     6061 tgcaccacac cgacgttggg ccgtccggct acgatgcgga cgatgaacgg cccacgtcac
     6121 ggcggccacc ttctcgccgc gctcctgagg caggagggcg tcgagcacct gttcaccctc
     6181 tccggaggcc acatcgcgcc catctacgac gggtgcgtgg ccgagggcat ccgcatcgtg
     6241 gacttccgcc acgagcaggc ggcggcccac gccgccgacg gttgggccag ggtcacgggg
     6301 cgcccagggg tggccgccgt cacggcggga cccggcgtca ccgacgcggt caccggcgtg
     6361 gccaacgcct tcttcgccga ctcgccgatg gtgctgatcg gcggccgcgc cccgctcgcc
     6421 gagcagcgga tgggctcgct ccaggagatg gaccacgtgg ccctgatgcg gtccatcacg
     6481 aagtgggccg acacgtgctg gcagacccgc cgcatcgcgg actacgccgc cgaggccctc
     6541 cgtcatgcca cgacgggtcg gcaggggccc gtcttcctcg acgtcccgct cgacgtgcag
     6601 ttcgacgtct ggccgacgga cgtggagctg ccgcggggct tccgcccgga gggcaggccc
     6661 cagggcgatc ccgacctcgt gaagcgcgcg gcgaagatgc tggccgacgc ggagcggccg
     6721 gtcgtcttcg ccggctccgg ggtccgctgg tcggaggccc acgaggccct ggccgccctg
     6781 gccgacgcgc tgaagatccc ggtgttcctg aactcgctcg ggcgagggtc cctgcccccg
     6841 gagcatcggt cgttcttctc cgcggcgcgg aagcacgcgc tggggaaggc cgatctcgtg
     6901 ctcgcgctcg gtgtggactg ggacttccgc ctggggttcg gtcggaggaa cctgcccgag
     6961 agcgtccgcg tgatccaggt ggacgtggac gggcggcaca tcgggcgcaa ccggccggtc
     7021 gacgtgggga tcgtgggcga ccccggccgc gtgatcgagc agctgctcgc cgagggggtg
     7081 ggctcgcccc aggagcccag ctggaccgag gaggtccggg acgtcgagcg ggccaagcac
     7141 gaggaggccc gggccgggat ggattccgac gcggtcccga tccacccgca gcggttcgcg
     7201 agggagatcc gggacttcct cgacgaggac gccaccgtgg tgggcgacgg cggcgacatc
     7261 gtcggcatca gctcctcgat cgtgcaggcg aggcggccgg gccactggct cgaccccggg
     7321 cccttcgggt gcctcggcgt aggaccgagc ttcgccatcg cggcgaagct ggcgaggccg
     7381 gacacgcagg tcgccgtgat ctacggcgac ggctcgttcg ggctcaacgc gatggagtac
     7441 gagagcgcca tccggcagga catcccgttc gtggggatca tcgggaatga cggcgcctgg
     7501 gggcagatca aggtggctca ggaggcgctc tacggcgagg ggaacgctcc ggcggcgacc
     7561 ctctccaccg aggtggggta cgagcggatg gtggagggtc ttggcggcta cggtgagcgt
     7621 gtgaccgatc cgagccagct cggcccggcg ctccggaggg cgttcgacag cggcgtggcg
     7681 gcgtgcctca acgtgatcgt ggacccgagc ctgatgcgcc gggcatcgta cctgggctga
     7741 atccttcatc cgcggtatgc tcggcgcccg ccttcgagac cgaggaggtg tgctcatgcg
     7801 gaagctgatc ccgatgctct ccgtcgtcct cgtccttgcc ctggcgtcgc cgggcggcgc
     7861 catcgtgaac ggcgagctcg acggggacga ccacccgaac gtcggggccc tgatcggcga
     7921 cttcgacggc gacaagatca tcatctgctc gggcacgctg atcgcccctg acgtgttcct
     7981 cacggcgtcg cactgcaccg cgttcctgga gtcgatcggc cagtcgacgg cctgggtgtc
     8041 gttcgacagc gagctggagg agcccgtcac gagcccgccg gccaccctgc atcaggggac
     8101 gctgcacacg cacccggact acgggttctc ggggcccggc ggcgtgtccg atacccacga
     8161 catcgccgtg gtcgttctgg atcagccggt gcagggcatc cagcctgctt ccctgccgac
     8221 cctcaaccag ctcggccagg ccgccgcgaa gaacggcctg aagcgccaga agttcaccgc
     8281 ggtgggctac ggcgtccacg aggcggttct cggcggcgga ccgccgtcgt tcccgttcga
     8341 cggtgcccgg tggcgggcgg tctccgagta ccgctcgctg aagaacgcct ggctccacct
     8401 gtcgcagaac ccctcgaccg gcgacggggg gacgtgcttc ggggactcgg gcggtccgaa
     8461 cttcctgggg gccggggcag gggagacgga cgtggtcgcc gggatcaccg tgatgggcga
     8521 ttcgatgtgc cgggccacga acgtgaccta ccggctcgac acggcctcgg cgcggaactt
     8581 cctggacgac ttcgtggcgc tgccctgagg acgcggcgcg gtcgcgggtc gcttcacacg
     8641 acccg
//
LOCUS       DASUTZ010000109        24093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1194750, whole genome shotgun sequence.
ACCESSION   DASUTZ010000109 DASUTZ010000000
VERSION     DASUTZ010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 24093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24093
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194750"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1268
                     /locus_tag="VF097_04920"
     CDS             150..1268
                     /locus_tag="VF097_04920"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEX6262172.1"
                     /translation="METALLHRAAPDPVAMLDECHRRAVDACAERLAWIAAIEERRLW
                     RRDGATSMTAWLAGRWGLARSTTREMVRVASALRSLPAIREAYEERRISWDQIRPLTR
                     FATPETDEAWSRRAPRMRPVSLWQEVRRHESLEREEEDSLHRRRYLRLDWDPERPLLH
                     LEGMLASEQGAALQAALGSRAERMETPRDALDPVGAKLADALVGSVTAAPADKAASPV
                     LVVHTAAELLARVPEPGRPHLAETESGVRLSAEAVRRLACHAKVEWVLETDGRPVGIG
                     RRMRAIPPRLWRLLRFRDRACRFPGCERGAWLKAHHIHHWARGGPTDLDNLVLLCHAH
                     HRLLHEGGWRLRGHPSSELAWLRPDGTRLVTSPEVLSS"
     gene            1283..2026
                     /locus_tag="VF097_04925"
     CDS             1283..2026
                     /locus_tag="VF097_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX6262173.1"
                     /translation="MHPSDGLRWASLAVALTIAGASGPPPERAAAVTTLAASSRTRPS
                     AAGPRAGEDRLASQASGFRFEIERVRRADLGRSWGPGCPVPPRQLRLLTVRHWGFDGE
                     ERLGRLVLRRRHARPMVRVMRRLFRVGYPIKRMRPVDRYGGSDRRSMRANNTSAFNCR
                     TATSSTSWSEHAYGRAIDLNPVQNPYVTASGRVLPRRGRRFVDRSVDHPAVIRAGDPV
                     VRTFARIGWSWGGDWSSTRDYQHFSSTGR"
     gene            2048..2923
                     /locus_tag="VF097_04930"
     CDS             2048..2923
                     /locus_tag="VF097_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262174.1"
                     /translation="MADDARRAFLHELIDDAGLFPPASLSMDAATAQHAGARGGPHAW
                     IQGRFICPAGRLSELAGHLGLLDRWRVSVLLDAAALDVGLEATAALLDVERGRVQIEL
                     VETKLPVTDHESVGDLVEWVEGADLGALPFLEVDPEPGLDDDLEAIAGHAAGAKLRTG
                     GPTEDHVPSPAAVAAFLDGCRRLGLRCKATAGLHHPFRHVDEETGFVQHGFVNVVGAA
                     VLAREHDLGPDRLEEIVAEETPNAFQLSADRFRWRDLDAGPEAVRDARGSLLFSYGSC
                     SFQEPVDDLLELGVL"
     gene            2920..4212
                     /gene="fahA"
                     /locus_tag="VF097_04935"
     CDS             2920..4212
                     /gene="fahA"
                     /locus_tag="VF097_04935"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HEX6262175.1"
                     /translation="MSDDPTVDPGLRSWVPVPEGSDFPIQNLPYGVFSRGVGPARAGV
                     AIGEHVLDLGELALGGLLDGISRLRPEIFLANALNPFLGLGRATWRAVRRRLSGLLVE
                     GDRRIGEAGLAERALVPLSEVELHLPFAPGDYVDFYSSIEHATNLGRMFRPEGDPLLP
                     NWRHLPVGYHGRSGTVVVSGTPIRRPVGQTAPAGGRAGPGWRPSRLLDLELEVGFVTG
                     PGNSLGRPIPIQRAADHIFGLVLVNDWSARDLQRWEYEPLGPFLGKSFATSISPWVVT
                     LDALAPWRVPSPPQDPEPLPYLRTDEPWGLDVELEVALRAEGAAAPSTISRTNLRGLY
                     WTIAQQLAHATSNGAVARPGDLFASGTISGADHGSQGSLIELTEGGKRPLELPGGVAR
                     TFLEDGDTVVLRAWCEAGRRRIGFGEVTGRIVPAEPVR"
     gene            4304..5464
                     /locus_tag="VF097_04940"
     CDS             4304..5464
                     /locus_tag="VF097_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HEX6262176.1"
                     /translation="MDIEIGIGKQARRGYGFDEVTIVPSRRTRDPDDIDISWEIDAWT
                     FPLPLMASAMDAVVSPATAIEVGRMGGLAVLNLEGLQTRYEDPDPLFDEVANLPPEKA
                     TRRMQELYQEPVKQELIVRRIKEIKEGGVVSAASLTPQRTARYAHLVLEAELDILVIQ
                     GTVVSAEHVSTQSEPLNLKEFIARFEIPVIVGGCASYSTALHLMRTGAVGVLVGVGPG
                     AACTSRQVLGIGVPQATAIADASAARMQHLHETGRYVHVIADGGMRTGGDIAKAIACG
                     ADAVMIGSPLAKAHEAPGQGSHWGMATFHPSLPRGTRVQTRQIGTLEEILVGPAHEND
                     GSLNLFGALRMSMATTGYLNIREFQKAEVMIAPSVATEGKLYQRQQGVGQGA"
     gene            complement(5469..7331)
                     /locus_tag="VF097_04945"
     CDS             complement(5469..7331)
                     /locus_tag="VF097_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX6262177.1"
                     /translation="MDAKPVQLPELATKAYGIWADRMLRSLPPSSRRGRIPTIAGGIL
                     DQIAAQASSAAGRGRRITPEISVPLELVDGIVRQLQRLSALTPRKDASPDLEEVGWLI
                     ELALRRAARAPAARPVSRPPQEPQAPELTPEEDGPPVEPAIEESPAPAVEAQQVEIPN
                     AVEKPAPAVEAQIDIPSADPGRDVLVEDESEMVEIDAGPDVLVEPETPGEEELVAAGI
                     EGSPNPIRVLLVDDDPRTLAMLGMALKGEHDIEVAGLAPDVSKAIEAARASQPDVAVV
                     DVRMPRGGGPRAARGILALSPATRILAYSALDERRAVLSMLEAGAVGYLVKGGPQEEV
                     AAAIRKAMMGERPMSEEVADEVMKALTERIRLEADDRDSREGLERQIRRFLAGRDLRM
                     AWQPIVQLYSRDVVGAEALPRFGGAPKRTPEGWFREAWRLGFGPALEGTVLEAAAGRL
                     EQLPEELFVAVNLSPGCLVEPGFLESMGHLPWERIIVEVTERSSVDDYAQLRAAAREI
                     RRHGGRLAVDDAGAAFASLRHVAEIEPDLVKMDARVCRNIHIDARMHALATALISFAR
                     ELGAEIVAEGIEHRGQLDALKDIGVEFGQGSFFTGVEDSKEVQVRVPRKRRARR"
     gene            complement(7408..9063)
                     /locus_tag="VF097_04950"
     CDS             complement(7408..9063)
                     /locus_tag="VF097_04950"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX6262178.1"
                     /translation="MNARGRDRGRAGYAPSPTPEILLPELPVHTYLHWCDLVRQVAVD
                     RGHATDDVLDAIERQAREAARTHRLSLRPRLSLGAETIAAARERIGRSLTRALDDHDS
                     DAEELEEPTLEIMLVLSREEPEGRPGSPSRTSANRETGSIRVLIADDDPRVRKVLATT
                     LEADEIEVAGVAADAEEAIRFAAAERPDVALLDVRMPGGGGGAAARGIAALSPETTIV
                     AHSALEDRQPILDMLRSGAVGYLLKGMDPQDLASAMRSAARGEPLIAAELVGHVVDEL
                     SQRVRSEARMDGVRENRARRIRRFAAGRGMRMVGQPIYELGSGLPVGIEALARFDSPP
                     HDPEPWFREAWDLGLGPELELAALQRAVAALPSFPEEVYLAVNLSPDTLLSKGFLEAK
                     SDLPWQRIVVEVTEHVPIADYERFREPVADLRRRGARIAVDDAGAGFASFHHVVELSP
                     DLIKLDRRLTLTIAGDERRRALARALVAFAEEVGTSVVAEGIETGETLRILRRFGVGF
                     GQGHFLAMPSSGALRPAALDLALRPAPARIVSDAAPRRRPPTW"
     gene            9239..10786
                     /gene="guaA"
                     /locus_tag="VF097_04955"
     CDS             9239..10786
                     /gene="guaA"
                     /locus_tag="VF097_04955"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX6262179.1"
                     /translation="MPLDVSEPRPVLVVDFGAQYAQLIARRIRECYVYSEIVPHDATL
                     EEVARRRPAGIVLSGGPASVYEEGAPVLNPHLLEAGVPVLGICYGQQAMTQALDGLVE
                     ATGAREYGKTELRVLEPGVLFREAPVEQSVWMSHSDTVVAPPEGFRVTARTEVTPAAA
                     FEDPERGLYGVQFHPEVSHTPHGPDLLKRFLYDACGLLPDWTPVNVIERAVERVREQV
                     GEAEAMCALSGGVDSAVAALLVHRAIGDRLTCVFVDTGLLREHEPEQVEEAFSRHFGV
                     PLVHVKAEDRFLARLEGVADPEEKRRRIGEEFIRVFEEVSREHRGARFLVQGTLYPDV
                     IESGSSTAATIKTHHNVGGLPERMDLELVEPLRDLFKDEVRVIGAELGLPEEVVHRQP
                     FPGPGLAVRVVGEVTRDRLALVRAADAIVREEMGRAGLEREIWQAFAILLDVRSVGVM
                     GDGRTYERPVVLRAVTSEDAMTADWARLPHDVLEKISTRIVREVPGVNRVAYDVTSKP
                     PGTIEWE"
     gene            complement(10838..11689)
                     /locus_tag="VF097_04960"
     CDS             complement(10838..11689)
                     /locus_tag="VF097_04960"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX6262180.1"
                     /translation="MAARRSWTRRARGPSTAGAVVVGVASGFLAGLFGVGGGILIVPG
                     LVFLGMRQRPAHGTSLAAIIPIAASGIVGFWIEGSVDWPVAAVITVGAATGAFLGTIA
                     LQRLEGRALRLGFALLLLATAARLFLEVPDRAGRADLDVPFIVGLLLVGLFAGVVAGL
                     FGVGGGVVIVPALVILFSIPDTVAKGTSLVVILPAALVGTARNLRYGNLDVAASLAVG
                     LSGVVTAFLSSKLAIRMDPRLSVVLFAGLLVAVTIRLLLVERQRTIARREGRWPPPEE
                     EAGLPEA"
     gene            11744..13894
                     /gene="pcrA"
                     /locus_tag="VF097_04965"
     CDS             11744..13894
                     /gene="pcrA"
                     /locus_tag="VF097_04965"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEX6262181.1"
                     /translation="MDSAILAPLNPVQREAVLHTEGPVLIVAGAGSGKTRILTHRIAY
                     LIKELGVPPGAILAITFTNKAAGEMAERVEELVGASVARRMWILTFHSTCARILRREH
                     ELLGLPSSFTIYDAADTERVVKMASGDLGIDEKRFPPRAIAAAIGRLKDVMLSPSDAA
                     ARAGNWYEKQVAEVYAEYQRRLQEAGALDFDDLILRTVRLFREHPEVLAHYQERFRYI
                     LVDEYQDTNRPQYHLVNLLASRYRNVCVVGDADQGIYSWRGATIQNLLDFEHDHPDAA
                     EFVLDQNYRSTRTILEVANALIDHNVQRKPKNLWTDAGGGDPLVRYRAEDEHEEAWYV
                     AQEIERLVEEEGFRYSDVAIFYRTNAQSRVLEDVLMKSGILYKVVGGLRFYERREIKD
                     VLAYLKALVNPQDVVATRRVINTPKRGIGDQTLAAVEAFARDEGITVLEAARRAEEIA
                     TISPRAVGALRGFVEVMDSLRSLLDEGASVARVVEAAYTESGYWATLDAERTVEAESR
                     KENLQELVGVGAEFDARFPDQGVPEFLEQVSLITEQDEYDETETGVTLMTLHTAKGLE
                     FDVVFIVGMEDGVFPHHRSMTDPAELEEERRLAYVGITRARRRLYLTHAWSRSLFGAS
                     SYNPPSRFLAEIPSELVHEVEGEGRTPREELLPPPVHTVEVVAGDTVRHDRFGEGVVV
                     AVTGRGSDAEVTIMFEDEGEKRLLLAYAPLEKVG"
     gene            complement(13899..14216)
                     /locus_tag="VF097_04970"
     CDS             complement(13899..14216)
                     /locus_tag="VF097_04970"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6262182.1"
                     /translation="MTDRVRIERAAPRLVRVSGELDMASSPELAEVLREIAGAGEGSL
                     VLDLSDLSFIDSTGLRVLIDVAVQLGESGDLILRNPTRPVMEVLEIAGVAEAAPNFVV
                     EAD"
     gene            complement(14277..14744)
                     /locus_tag="VF097_04975"
     CDS             complement(14277..14744)
                     /locus_tag="VF097_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262183.1"
                     /translation="MARPRIRFRSPGVKPGQKIGPLGPFRGRLGLQWVIAPIVLGVIL
                     LAAGWLFLRGREPSPPWRPVTDVASLPVGDGRETVPGVFVGRLSDGRVIAVAERAGCP
                     LEPSEDGYADCEGATYGLDGLPKDRGDPLDLVPVRIHDGVLYADPSRRVVRGP"
     gene            14830..15240
                     /locus_tag="VF097_04980"
     CDS             14830..15240
                     /locus_tag="VF097_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020496429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEX6262184.1"
                     /translation="MPGKHRILIAKPGLDGHDRGAKVVARALRDAGYEVIYTGLHQTP
                     EQIVEAAIQEDVDAVGLSIHSGAHMTLFPKVAAALRERGADDVVVFGGGIIPKEDVSA
                     LQEAGIERVFTPGAPTSSIVEWLEERLGAGEETS"
     gene            15241..16383
                     /gene="sucC"
                     /locus_tag="VF097_04985"
     CDS             15241..16383
                     /gene="sucC"
                     /locus_tag="VF097_04985"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX6262185.1"
                     /translation="MDLYEHQGKDLFGAHDIALVDGEIATAPEEARSIAERYGGAVAV
                     KVQVQVGGRGKAGGIALVTGADEAEEAARRMLEEGFRGTRVTRVLVERLVDIETQYYM
                     AITLDRSSRRYLAMTSNEGGMEIEDIAKEKPDAIRRSEIDPVLGLMPYQTRYLVGHFP
                     EAARKGSADILGKLYGVLTAADATLVEVNPLALLTDGAVVALDAKVTLDDNALFRHPD
                     LAELAKDFVLDPVEARAKELGLQYVKLDGEVGIIGNGAGLVMSTLDVVDQAGGSAANF
                     LDAGGGAGADVMAASLEVILSDPAVRSVLVNIFGGITRGDRVAQGILEALGRIEVEVP
                     IVVRLDGTNAEEGRRMLAEAEHDRIIPAATMLEAAEKAVAAAKESS"
     gene            16383..17252
                     /gene="sucD"
                     /locus_tag="VF097_04990"
     CDS             16383..17252
                     /gene="sucD"
                     /locus_tag="VF097_04990"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX6262186.1"
                     /translation="MAVLVTEDTRLVVQGITGKEGAFHTLRNRDYGTPIVAGVTPGKG
                     GDSVDGIPVFDSVREAVEETGANTSLTFVPARFAADAILEAADAGVDLVVCITEGIPV
                     QDMARVWNYMLGKDCRLVGPNCPGVISPGKANIGIIPHEITKAGGVGLVSRSGTLTYQ
                     IMNELTQQGIGQSTCIGIGGDPIIGTGFVDALELFEADPETEAIVVIGEIGGDDEEKA
                     AAHIAEHVSKPVVGYVAGFTAPPGKRMGHAGAIITGSKGTAKAKAEAFEEAGVPVGRN
                     PTEVAQIVASIRS"
     gene            17610..19505
                     /locus_tag="VF097_04995"
     CDS             17610..19505
                     /locus_tag="VF097_04995"
                     /inference="COORDINATES: protein motif:HMM:NF040070.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6350 family protein"
                     /protein_id="HEX6262187.1"
                     /translation="MTGEPRLSAGIMRALLLGALVFLVMLLLSAVIAFAAFFTSDADV
                     AEVAKVGGVIFFSFHHVGLRTELPTFDPSAVPGAGATQGGLPLGPIDLSFTIWAALLL
                     GTLLALVLLYRAGRRAAEEGGGSTGARILQGAFVAVPYAVLAALLSLVVRFELDLGPQ
                     LASFFGSAGAVEVSAPLLSALLWPLGIAVVAGGLGGYGAARRDAAAGTPGRLGAMVSG
                     AWRMLLALLVAGFVGLLILGAINPDLTASYFETVGSGRSGLVLLLATIAAVPNLALWG
                     ITLGMGGSIVAGDLLGADAVRIASLLEFPTDLGLQLAGGSLVGGTPQVSTEVAPIEYF
                     AFIVLTLIALVIGGRMAARRAHVVSAGQGALIGAGSGVLFAIAFFGALALAGITVNTS
                     VQAGQDGLVRMGPGLLLGGLLGLAWGVVAGAIGGATGSGAEAPFPIDRRPAPAPAPPG
                     EGPEATQELPQQPATGTGFEGLAAPRAAVRPEPEPEPEAGPAPEPEQGALAEEEEASA
                     PLQAPTEHASGEPPAFEFTTPQAQWRAPEPEPPAEGEAPAGPEEPGGGSAPEPDQEEP
                     TGEQGPPQTPGWTAPERDAEPPSEAPPWSDPADFEPRPWPGDEPEPGPSEEVDDDEGE
                     EEGPAPA"
     gene            complement(19563..20030)
                     /locus_tag="VF097_05000"
     CDS             complement(19563..20030)
                     /locus_tag="VF097_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262188.1"
                     /translation="MAQQGFDISRLTMGQKTLLITGLVFLVTLFFPWESFDVGGLPGF
                     DLPGVNISGWNGIGVLAGILAILLLVWELMLMAGVNLNLNVSEALISAGLAAATVLFG
                     LIRFIIALTRSGYGIAWGAWVGLIVLLAMAYGAWVRFNESKAMGMPPPAPPAA"
     gene            complement(20104..20652)
                     /locus_tag="VF097_05005"
     CDS             complement(20104..20652)
                     /locus_tag="VF097_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262189.1"
                     /translation="MSASACSVHPDVPARETCGRCGRAACLGCAVPVRGEVRCMDCVG
                     EELGEPIEAAPPPRSVRFRDLPAGLLFLAAVLVSLLPWDRQGSHAGFLSAWTPVPEPW
                     PFVAGVLLLLATVASLRGRQGRLRAVHIALGALAVLATILALPAPELSTRGPVPIVVL
                     GLAVAATLTGAVRATAAFWQRP"
     gene            20676..21281
                     /gene="purN"
                     /locus_tag="VF097_05010"
     CDS             20676..21281
                     /gene="purN"
                     /locus_tag="VF097_05010"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX6262190.1"
                     /translation="MNARLAVLASGSGTNLQALLDDPVVGPRVSLVVSDRGDAGALDR
                     AAAAGVKAVHLDPAIHDERTAYDAALQDLLVSEGIDLVCLAGFMRVLGPTLVRSFEGR
                     MLNVHPSLLPAFPGAHAPRDALKWGAKVTGVTVHVVDEEVDHGPIVLQEPVPVREDDD
                     PESLHARIKDVEHRVYPLAVRWWLEGRVRVEGRRVRIEGEA"
     gene            21278..22819
                     /gene="purH"
                     /locus_tag="VF097_05015"
     CDS             21278..22819
                     /gene="purH"
                     /locus_tag="VF097_05015"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX6262191.1"
                     /translation="MSDLLPVRRALVSVADKEGLIALGRGLVEEGVTIVSTGSTARAL
                     QEEGVPVTSVAEVTGFPEMLGGRVKTLHPAIHAGILADKRDEEHLRQLEEAGIKPFDL
                     VVVNLYPFERTVRSGADRDEVVEQIDIGGPTLVRAAAKNFGSIGVVVDPERYGELLAA
                     VEGGGLPERLRASLAEEAFRHVALYDAAIAAWFGSWAGEGELLPRVLAPGFELASPLR
                     YGENPHQRAGLYRAALSDGPLGGAEVLQGKEMSYNNWLDAEAARALASSFEEPACVIV
                     KHHNPCGAAATGDLADCYRDALASDPVSAFGGIVAFNREVDEAAARAMEEVFTEVVVA
                     PGFSSEARQLLAGRKSLRVVLAPPPAPGGLELRPIEGGALVQDADAVRELRDAMRVAT
                     RRSPTEEEWEGLLFAWRVAARVKSNAIVLARGRATLGVGAGQMSRVDSVELARRKAGD
                     RAMGSVLASDAFFPFRDGIDAAAAAGVTAAIQPGGSVRDEEVVAAADEHGMAMVLTGV
                     RHFRH"
     gene            22943..24064
                     /locus_tag="VF097_05020"
     CDS             22943..24064
                     /locus_tag="VF097_05020"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX6262192.1"
                     /translation="MRRGTLARLGALVAVVALLAAACQRGEDAPEATDEEEPQAQQEG
                     GLLQEIRERGMLRCGVNNTVPGFGFETSGGEIEGFDVDFCTAIAAAVFNTPDVEGMIE
                     LFPVDAEERFNALRAGEYDVLVRNTTWTSSRDGSEGVAFAHVNFYDGQAMMVREGEFN
                     AVDEMDGTSVCVTTGTTTELNLADYAAQVGVDIEPVTFAENPQLQEAFISGRCDGWTS
                     DRSQLAGIRSAWPDEEGGPESLVILDDIMSKEPLAPGVLDGDQEWYDAVNWVVNGLIL
                     AEELGVDSSNVEQQAENPDDPQIAALLGVSFEAETETITVESGLGLEDDFMQAVLQAV
                     GNYGEIFDRHIGPDSPLGLDRGVNALWTDGGIQYAMPFR"
BASE COUNT         3388 a         8496 c         8911 g         3298 t
ORIGIN      
        1 gcgaaccgcg ccacggtctt cgtcggcctg atcccgtggg aggagggctc cggcggcccc
       61 tgccgggtga tcgcgcgctg gtaggatcgc cgcgtttcgg gcgggtctga cgagcggttc
      121 atgtcagacc cgcctgctac gatgcggcca tggagacggc gctgctgcac cgagccgccc
      181 ccgatccggt ggcgatgctg gacgagtgtc acaggagggc tgtcgacgcc tgcgccgagc
      241 ggctcgcgtg gatcgctgcc atcgaggagc gacggctctg gcggcgcgac ggggccactt
      301 cgatgaccgc ctggctcgcg gggaggtggg gcctggcccg atcgaccacg cgcgagatgg
      361 tgcgggtggc ctcggcgctt cgctcgctcc ccgcgattcg ggaggcctac gaggagcgac
      421 ggatctcatg ggaccagatc cgtcccctca cgaggttcgc cacgcccgag accgatgagg
      481 cgtggtcgcg gagggctccc cggatgcgcc ccgtgagcct ctggcaggag gtgcggcgcc
      541 acgagagcct ggagcgggag gaggaggact cgctccatcg ccgccggtac ctccggttgg
      601 actgggaccc cgagcgtcca ctgctccacc tcgaggggat gctcgcctcc gagcaggggg
      661 cggcgctcca ggcggcgctg gggagccggg ccgagcggat ggagaccccc cgcgacgcct
      721 tggatcccgt cggggcgaag ctggccgacg cgctcgtggg gtccgtcacc gcggcccccg
      781 cggacaaggc ggcgtcgccg gtgctggtag tgcacacggc ggccgagctc ctggcgcggg
      841 tccccgagcc ggggcggccg catctggccg agaccgagag cggggtccgg ctgtccgcgg
      901 aggccgtccg caggcttgcc tgccacgcca aggtcgagtg ggtgttggag acggacggcc
      961 gccccgtggg gatcgggagg cggatgcggg ccatcccccc gcggctgtgg cggctcctcc
     1021 gcttccggga ccgcgcctgc cggttcccgg ggtgcgagcg cggcgcctgg ctgaaggccc
     1081 accacatcca ccactgggcg cggggcgggc ccaccgacct ggacaacctc gtgctcctgt
     1141 gccacgcgca ccacagactg ctgcacgagg gggggtggag gctccggggc catccctcga
     1201 gcgagctggc ctggctccgg cccgacggca cgcggctcgt gacctctccc gaggtcctct
     1261 cgtcctgagg catgatgggg ccgtgcatcc atcggacggc ctccggtggg cctccctcgc
     1321 ggtcgctctg accatcgccg gcgcgtcggg cccccctccc gagcgtgccg ccgccgtcac
     1381 gaccctcgcc gcctcgagca ggactcggcc cagcgccgcg ggtccgcgcg ccggcgagga
     1441 ccgtctcgcc tcgcaggcct ccggcttccg gttcgagatc gagcgggtga ggcgagcgga
     1501 cctcggaagg tcgtgggggc cgggctgccc ggtgccgccc cggcagctca ggctcctcac
     1561 ggtccggcac tgggggttcg atggggagga gcggctcggg cgactggtcc tgcgtcgcag
     1621 gcacgcccgg cccatggtcc gggtgatgcg gcgcttgttc cgcgtcggct acccgatcaa
     1681 gcggatgcgg cccgtggacc ggtacggggg cagcgaccgc cgctcgatgc gggcgaacaa
     1741 cacctccgcg ttcaactgcc gcacggcgac gagctccacc agctggtcgg agcatgcgta
     1801 cgggcgagcg atcgacctca acccggtcca gaacccgtac gtcaccgcct cgggacgcgt
     1861 gctgcccagg cgggggcgcc gcttcgtcga ccggtcggtg gaccaccccg cggtcatccg
     1921 ggcgggggat ccggtggtgc ggacgtttgc ccggatcggg tggtcgtggg gcggggactg
     1981 gagctcgacc cgggactacc agcacttctc cagcaccggg cgctgacggg cgcctagaat
     2041 cctgcgcatg gcggacgacg ctcgccgagc cttcctgcac gagctgatcg acgacgcggg
     2101 gctcttccct cccgcgtcgc tgtcgatgga tgccgcgacg gcccagcacg cgggggcgcg
     2161 ggggggcccg cacgcatgga tccagggcag gttcatctgt ccggcgggga ggctctcgga
     2221 gctggccggt cacctggggc tgctcgatcg atggcgggtc agcgtcctgc tggacgcggc
     2281 cgcgctcgac gtcggcctcg aggccacggc cgccctcctc gacgtcgagc gcggccgcgt
     2341 ccagatcgag ctcgtggaga cgaagctgcc ggtcacggac cacgagtcgg tgggcgacct
     2401 ggtggagtgg gtagagggcg ccgacctggg agccctcccc ttcctggagg tggaccccga
     2461 gcccgggctg gacgacgacc tcgaggccat cgccggccac gcagccggcg cgaagctgcg
     2521 caccggcggc cctaccgagg accacgtccc ctctccggcc gccgtggccg cgttcctgga
     2581 cgggtgtcgg cggctcgggc tgaggtgcaa ggccacagcg gggctgcacc atcccttccg
     2641 gcacgtggac gaggagaccg gcttcgtcca gcacggcttc gtcaacgtcg tcggggccgc
     2701 cgtcctggcc cgcgagcacg acctcggccc cgatcggctg gaggagatcg tggcggagga
     2761 gaccccgaac gccttccagc tctcggcgga ccgcttccgg tggcgggacc tcgacgccgg
     2821 acccgaggcc gtccgggatg cccgggggtc cctcctcttc tcctacggca gctgcagctt
     2881 ccaggagccc gttgacgacc tcctcgagct cggggtgcta tgagcgacga tccgacggtg
     2941 gacccgggcc tccgctcgtg ggtcccggtt cccgagggct cggacttccc gatccagaac
     3001 cttccctacg gggtcttctc caggggcgtg ggcccggcgc gggcgggcgt ggccatcggg
     3061 gagcacgtcc tggatctcgg ggagctcgcc ctgggcggcc tcctcgacgg gatctcccgg
     3121 ctccggcccg agatcttcct cgcgaacgcc ctcaacccct tcctcggcct gggccgcgcc
     3181 acctggcgcg cggtgcgccg gcgcctctcg ggcctgctgg tcgaggggga tcgccggatc
     3241 ggggaggcgg ggctcgccga gcgggccctg gttccactct ccgaggtcga gctccacctc
     3301 cccttcgccc ccggggacta cgtcgacttc tactcgtcga tcgagcacgc caccaacctc
     3361 gggcggatgt tccgtcccga gggtgatccg ctgctcccca actggcgcca cctccccgtc
     3421 ggataccacg ggcggtcggg gaccgtggtg gtgagcggga cgcccatccg gaggccggtg
     3481 gggcagacgg cgccggccgg cggcagggca gggccgggct ggagaccctc gcgcctcctc
     3541 gacctcgagc tggaggtggg gttcgtgacc ggcccgggca actctctggg ccgtccgatc
     3601 cccatccagc gcgcggccga tcacatcttc gggctggtgc tcgtcaacga ctggagtgcc
     3661 cgcgacctcc agcgctggga gtatgagccc ctcgggccgt tcctggggaa gtcgttcgcc
     3721 acctcgatct cgccgtgggt ggtcacgctc gacgcgctgg ccccctggag ggtcccgtcc
     3781 ccgccccagg atcccgagcc cctcccgtac ttgcggacgg acgagccctg ggggctcgac
     3841 gtggagctgg aggtggcgct gagggccgag ggcgccgccg cgccctcgac gatatcccgg
     3901 accaacctcc gaggcctcta ctggacgatc gcccagcagc tcgcgcacgc cacgtcgaac
     3961 ggagccgtag cccgcccggg cgacctgttc gcctcgggga cgatctccgg cgccgaccac
     4021 ggatcgcagg gcagcctgat cgagctcacg gaggggggga agcgtcccct cgagctgccc
     4081 ggcggcgtgg cgcggacctt cctcgaggac ggggacaccg tggtcctgcg cgcgtggtgc
     4141 gaggcggggc ggcgccggat cggcttcggc gaggtcaccg gccgcatcgt gcccgccgag
     4201 cccgtccgtt gaagtcgcgg tgggccgctg gtaccgtgcg ggcatgtgac ccgacacccg
     4261 gccgcgctca tcggccgggc gtcttcgtct ccgagggggg aacgtggaca tcgagatcgg
     4321 catcgggaag caggctcggc gggggtacgg gttcgacgag gtgacgatcg tcccctcgcg
     4381 gaggacccgg gaccccgacg acatcgacat ctcctgggag atcgacgcct ggacgttccc
     4441 cctcccgctc atggcctccg ccatggacgc ggtcgtctct ccggccaccg cgatcgaggt
     4501 gggtcgcatg gggggcctcg ccgtcctcaa cctcgagggc ctgcagacgc gatacgagga
     4561 ccccgacccg ctcttcgacg aggtggcgaa cctccccccg gagaaggcga cgcgccggat
     4621 gcaggagctc tatcaggagc cggtcaagca ggagctgatc gtccgtcgga tcaaggagat
     4681 caaggagggc ggggtcgtct cggccgcctc gctcacgccg cagcggacgg cgcggtacgc
     4741 gcacctcgtc ctcgaggccg agctcgacat cctcgtgatc caggggacgg tggtctccgc
     4801 cgagcacgtc tccacgcaga gcgagcccct caacctcaag gagttcatcg ccaggttcga
     4861 gatcccggtg atcgtcgggg ggtgcgcgtc gtactcgacc gcgctccacc tgatgcggac
     4921 gggcgcggtc ggcgtcctgg tcggggtggg accgggcgct gcgtgcacct cgcgccaggt
     4981 tctcgggatc ggcgtcccgc aggccacggc gatcgccgac gcctcggccg ctaggatgca
     5041 gcacctccac gagacgggcc gctacgtcca cgtgatcgcc gacggcggga tgcggaccgg
     5101 cggcgacatc gccaaggcca tcgcatgcgg ggccgacgcc gtcatgatcg ggtcgccgct
     5161 ggcgaaggcg cacgaggcgc cggggcaggg ttcccactgg ggcatggcca cgttccaccc
     5221 gtcgctgccc cgagggacca gggtgcagac ccggcagatc ggaacgctgg aggagatcct
     5281 cgtgggcccg gcccacgaga acgacggctc cctcaacctc ttcggggcgc tccggatgtc
     5341 gatggccacc accggctacc tgaacatccg ggagttccag aaggccgagg tgatgatcgc
     5401 cccctcggtc gccacggaag ggaagctcta ccagcgccag cagggggtcg gccagggagc
     5461 ttgaagcgtc agcgccgagc gcggcgcttc ctggggaccc ggacctggac ctccttcgag
     5521 tcttccaccc cggtgaagaa ggagccctga ccgaactcca cgccgatgtc cttgagggcg
     5581 tccagctgcc cccggtgctc gatcccttcg gccacgatct cggcgcccag ctcacgggcg
     5641 aacgagatca gggcggtggc cagcgcgtgc atgcgcgcat cgatgtggat gttgcggcac
     5701 acccgcgcgt ccatcttcac caggtcgggc tcgatctcgg cgacgtgccg gaggctggcg
     5761 aaggcggccc ctgcgtcgtc gaccgcgagc cggcccccat gccggcggat ctcccgcgcc
     5821 gccgcccgga gttgcgcgta gtcgtccacc gacgaccgct cggtcacctc cacgatgatc
     5881 cgctcccagg ggaggtggcc catcgactcc aggaagcccg gctccacgag gcacccaggg
     5941 gagaggttga ccgcgacgaa gagctcctcg ggcagctgct ccagccggcc ggcggcggcc
     6001 tcgaggaccg ttccctcgag cgcggggccg aaccccagcc tccacgcctc gcggaaccat
     6061 ccctcgggcg tgcgcttcgg ggcgcccccg aaccggggga gggcctcggc cccgacgacg
     6121 tctcgggagt agagctgcac gatcggctgc caggccatgc ggaggtcccg cccggccagg
     6181 aagcgtcgga tctgacgttc cagcccctcc ctcgagtcgc ggtcgtcggc ctccaggcgg
     6241 atccgctcgg tgagagcttt catcacctcg tcggccacct cctccgacat cggacgctcg
     6301 cccatcatcg ccttgcggat cgcggcggcc acctcttcct gcggcccgcc cttcaccagg
     6361 tagccgacgg cgccggcctc gagcatcgac agcaccgccc tgcgttcgtc gagcgccgag
     6421 tacgcgagga tcctcgtcgc gggggagagg gccaggatgc cgcgcgcggc gcggggaccg
     6481 ccgccccgcg gcatgcgcac gtccaccacg gcgacgtccg gctgagaggc gcgcgccgcc
     6541 tcgatcgcct tggagacgtc gggggcgagg ccggcgacct cgatgtcatg ctcacccttg
     6601 agggccatcc ccagcatggc gagcgtgcgg gggtcgtcgt cgaccaggag gacccggatc
     6661 gggttcggcg atccctcgat ccctgcggcg acgagctcct cctcgcccgg ggtctccggc
     6721 tccacgagga cgtccggccc ggcgtcgatc tcgaccatct cggactcgtc ctcgacgagg
     6781 acatcccggc ccgggtcggc gcttgggatg tcgatctgcg cctcgacggc gggcgcgggt
     6841 ttctcgacgg cgttcgggat ctcgacctgc tgcgcctcga cggcgggcgc gggcgactcc
     6901 tcgatcgcgg gctcgaccgg cggaccatcc tcctcgggcg tgagctccgg ggcttgcggc
     6961 tcctgggggg gccgggagac cggccgcgcg gccggggccc gcgcggcccg gcggagggcg
     7021 agctcgatca gccagcccac ctcctcgagg tcgggggagg cgtccttccg cggtgtgagg
     7081 gcgctgagtc gctgcagctg gcggacgatt ccgtccacga gctccagggg gaccgagatc
     7141 tcgggggtga tacgccggcc acggccggcc gcgctcgagg cctgggcggc gatctggtcg
     7201 aggatcccgc cggcgatggt cgggatgcgg ccgcgccggg aggacggggg aaggctccgc
     7261 agcatgcggt cggcccagat gccgtaggct ttcgtggcga gctcgggaag ctgaacgggc
     7321 ttggcgtcca tgtgaacccc cttgctctcc tccatcggcg gccgggacct cagcggtaca
     7381 gtcggtcgga cgggtgagat ccgcgcctca ccacgtcggg ggacgccgtc tcggagccgc
     7441 atccgacacg atccgcgccg gcgcgggcct gagggcgagg tcgagggccg ccggccggag
     7501 cgcgcccgag gacggcatgg ccaggaagtg cccctggccg aagcccacgc cgaaccgccg
     7561 gaggatccga agcgtctctc ccgtctcgat cccttccgcc accacggacg tccccacctc
     7621 ctcggcgaac gcgaccagcg ctcgggccag cgcccgccgc cgctcgtccc cggcgatcgt
     7681 cagcgtgagc ctccgatcga gcttgatcag gtcgggcgag agctccacga cgtgatggaa
     7741 cgaggcgaac cccgctcccg cgtcgtctac cgcgatccga gcgcccctcc tccgcaggtc
     7801 cgccaccggc tctcggaagc gctcgtagtc cgcgatcggc acgtgctcgg tcacctccac
     7861 gacgatccgc tgccacggga gatcgctctt cgcctcgagg aaccccttcg acagcagcgt
     7921 gtccggggag aggttgaccg ccagatacac ctcctcgggg aacgagggga gggcggcgac
     7981 ggcgcgctgc agcgccgcca gctcaagctc cgggccgagc cccaggtccc aggcctcccg
     8041 gaaccagggc tcggggtcgt ggggaggcga gtcgaaccgc gccagggcct cgatacccac
     8101 ggggaggccg ctccccagct cgtagatcgg ctggcccacc atccgcatcc cccgccccgc
     8161 ggcgaaccgc ctgatccgac gggcccgatt ctcccggacg ccatccatcc tggcctccga
     8221 ccgcacgcgc tgcgagagct cgtccaccac gtgccccacc agctcggcgg cgataagggg
     8281 ctccccgcga gcggcgctcc gcatcgcgct ggcgaggtcc tgcgggtcca tccccttgag
     8341 caggtagccg accgcccccg atcgcagcat gtccaggatc ggctgtcggt cctcgagggc
     8401 cgagtgggcc acgatcgtgg tctcgggcga gagagcggcg atgcctcgcg ccgccgcgcc
     8461 tccgccgccg ccgggcatcc gcacgtcgag cagggcgacg tcgggacgct cggccgccgc
     8521 gaaccggatc gcctcctccg cgtcggcggc gaccccggcc acctcgatct cgtcggcttc
     8581 gagggtcgtc gcaagcacct tgcgaaccct ggggtcgtcg tcggcgatca gcacgcggat
     8641 cgaacccgtc tcccggttgg cggaggtccg ggagggggac ccagggcgtc cttccggctc
     8701 ctcccgcgac agaaccagca tgatctccag cgtgggctcc tccagctcct cggcgtcgga
     8761 gtcgtggtcg tccagcgccc gggtgagcga tcggccgatc cgctcgcggg cggcggcgat
     8821 cgtctcggct ccgagagaga gacgagggcg gaggctgagg cgatgggtcc gcgcggcctc
     8881 gcgggcctgg cgctcgatcg cgtcgaggac gtcgtcggtg gcgtgccccc ggtcgaccgc
     8941 gacttgcctc accaggtcgc accagtgcag gtaggtgtgg accgggagct ccggcaggag
     9001 gatctcgggc gtcggcgagg gcgcatagcc cgcgcgtccc cggtcgcgtc cgcgtgcgtt
     9061 catccgacct cgatcgtggg gctcggggcc gcagcgggcc agtcgcgcga tggggggttt
     9121 ccgggtctgc cccgtccgcc ccacgccggt gggccggacg accgaaggac gcacgtcctg
     9181 cgccggatgc agaacgggcg ttgaagcggt gtggacccag tggtacggtc gcggcacggt
     9241 gcccctggac gtctcagagc ctcgaccggt cctcgtcgtc gacttcgggg cgcagtacgc
     9301 acagctcatc gcccgacgga tcagggagtg ctacgtctac tcggagatcg tcccccacga
     9361 cgccaccctc gaggaggtgg ctcggcgtcg accggcgggc atcgtgctgt cgggtgggcc
     9421 cgcctccgtg tacgaggagg gtgcgccggt gctcaacccc cacctgctcg aggcgggcgt
     9481 gccggtgctg ggaatctgct acggccagca ggccatgacc caagccctgg acggcttggt
     9541 cgaagccacc ggcgcgcgcg agtacggtaa gaccgagcta cgcgtgctcg agccgggtgt
     9601 cctcttccgc gaggcgccgg tcgagcagtc ggtgtggatg tcgcacagcg acaccgtggt
     9661 ggccccgcct gagggcttcc gggtcacggc gcggaccgag gtcacacccg cggccgcatt
     9721 cgaggaccct gagcgagggc tgtacggtgt gcagttccat ccggaggtct cgcacacgcc
     9781 gcacggcccc gatctcctga agcggttcct gtacgacgca tgcgggctgc tcccggactg
     9841 gacgccggtg aacgtgatcg agcgcgccgt ggagcgggtg cgcgagcagg tgggcgaggc
     9901 cgaggcgatg tgcgcgctct cgggtggggt ggactcggcc gtggccgccc tcctcgtcca
     9961 ccgggcgatc ggtgaccggc tcacgtgcgt cttcgtggac accggtcttc tgcgcgagca
    10021 cgagcccgaa caggtggagg aggcgttctc ccgccacttc ggggttcccc tcgtgcacgt
    10081 gaaggccgag gaccggttcc tggcgcggct ggagggtgtg gccgaccccg aggagaagcg
    10141 ccggcgcatc ggcgaggagt tcatccgcgt cttcgaggag gtgtcccggg agcatcgggg
    10201 ggcgcggttc ctggtccagg ggaccctcta cccggacgtg atcgagtcgg gctcgagcac
    10261 ggccgccacg atcaagacgc accataacgt gggcgggctt ccggagcgta tggacctcga
    10321 gctcgtggag cctctccggg acctcttcaa ggacgaggtg cgcgtgatcg gcgccgagct
    10381 ggggcttccc gaggaggtcg tccaccgtca gccgttcccc gggccgggcc tggcggttcg
    10441 ggtggtgggg gaagtgacgc gcgaccgcct cgcgctcgtg cgggccgcgg acgcgatcgt
    10501 acgcgaggag atggggcggg ccggcctgga gcgggagatc tggcaagcct tcgcaatcct
    10561 gctcgacgtc cggtcggtgg gcgtcatggg cgacggcagg acctacgagc gtcccgtggt
    10621 gctgcgcgcg gtcacctccg aggacgcgat gaccgccgac tgggcccggc tcccgcacga
    10681 cgtcctggag aagatctcga cgcggatcgt ccgcgaggtg cccggggtga accgggtggc
    10741 ctacgacgtc acgtccaagc cccccggcac gatcgagtgg gagtgatcgg gccaggcctt
    10801 tgatgaggac tgggtcaggc tcggccgcgg ggtgacctca ggcctcgggc agcccggcct
    10861 cctcctcggg cggcggccac cgtccttccc tcctcgcgat cgtccgctgg cgctccacga
    10921 ggaggaggcg gatcgtcacc gccacgagca gcccggcgaa caggaccacc gagaggcgcg
    10981 ggtccatccg gatcgcgagt ttcgacgaga ggaaggccgt gaccacgccg ctcagcccga
    11041 cggccaagga ggcggccacg tccaggttcc cgtagcggag gttccgagcc gtccccacga
    11101 gcgcggcggg caggatcacc acgagcgagg tgcccttggc cacggtgtcg gggatggaga
    11161 agaggatcac gagggctggc acgatcacca cgccgcctcc caccccgaac aggccggcga
    11221 caacgccggc gaacaggccc acgagcagca ggcccacgat gaagggcacg tcgagatcgg
    11281 ccctcccggc ccggtccgga acctccagga agaggcgcgc ggccgtggcc agcagcagca
    11341 gggcgaaccc cagccgcagc gcgcgaccct ccagccgctg gagggcgatc gtcccgagga
    11401 acgcgcccgt ggcggccccc acggtgatca cggccgcgac cggccagtcc acagacccct
    11461 cgatccagaa gcccacgatc ccggaggccg cgatcgggat gatcgcggcg agcgaggtcc
    11521 cgtgggccgg tcgctgtcgc atccccagga acaccagccc cgggacgatg aggatgcccc
    11581 cgccgacccc gaagagcccc gcgaggaagc ccgaggccac ccccacgacg actgcgccgg
    11641 ccgtggaggg cccgcgagcc ctccgcgtcc aggaacgcct cgccgccacg cggcgccagt
    11701 gtggtcgcga ggaggggacg gagggcggac ctataatcgc gacgtggact cggcgatcct
    11761 ggccccgctg aaccccgttc agcgcgaggc cgtcctgcac acggaggggc cggtgctcat
    11821 cgtggccggg gcgggctcgg ggaagacgcg gatcctcacc caccgcatcg cctacctgat
    11881 caaggagctg ggcgtccctc ccggggccat cctggcgatc accttcacca acaaggccgc
    11941 gggcgagatg gccgagcggg tcgaggagct cgtcggcgcc tcggtggctc gacggatgtg
    12001 gatcctcacc tttcactcca cgtgcgcccg gatcctgcga agggagcacg agctgctggg
    12061 gctcccttcg agcttcacga tctacgacgc ggccgacacc gagcgggtgg tgaagatggc
    12121 ctcgggggac ctggggatcg atgagaagcg cttcccgccc cgcgccatcg cggcggcgat
    12181 cggacggctg aaggatgtga tgctctcgcc gtcggatgcg gcggcgcggg ccggcaactg
    12241 gtacgagaag caggtggccg aggtctacgc cgagtaccag cggcggctgc aggaggctgg
    12301 agccctcgac ttcgacgacc tgatcctccg gacggtgcgg ctgttccgcg agcaccccga
    12361 ggtcctcgcc cattaccagg agcggttccg ctacatcctc gtggacgagt accaggacac
    12421 gaaccgcccc cagtaccatc tcgtgaacct gctcgcgtcc cggtaccgca acgtctgcgt
    12481 ggtgggcgat gcggaccagg ggatctactc gtggcgcggc gcgacgatcc agaacctcct
    12541 cgacttcgag cacgaccacc ccgacgctgc cgagttcgtc ctcgaccaga actaccgatc
    12601 gacgcggacg atcctggagg tggcgaacgc gctgatcgac cacaacgtcc agcggaagcc
    12661 aaagaacctc tggaccgacg ccggtggggg cgatcccctc gtgcgctacc gggccgagga
    12721 cgagcacgag gaggcatggt acgtggcgca ggagatcgag cgcctcgtgg aggaggaggg
    12781 gttccgctac tcggacgtcg cgatcttcta ccgtaccaac gcccagtccc gggtcctcga
    12841 ggacgtgctg atgaaatcgg ggatcctgta caaggtcgtg ggggggctcc gcttctacga
    12901 gcgccgcgag atcaaggacg tcctggccta cctcaaggcg ctggtgaacc cccaggatgt
    12961 ggtggccacc cgccgcgtga tcaacacgcc gaagcgaggg atcggggacc agaccctggc
    13021 cgcggtcgag gccttcgccc gggacgaggg gatcacggtg ctggaggccg cgcgccgggc
    13081 cgaggagatc gccaccatct ctccccgagc ggtcggcgcc ctccggggct tcgtcgaggt
    13141 gatggactcg ctgcgctccc tcctggacga gggggccagc gtggcgcggg tggtcgaggc
    13201 cgcctacacg gaatccgggt actgggccac gctcgacgcc gagcggaccg tggaggccga
    13261 gagccgcaag gagaacctcc aggagctggt gggggtgggg gccgagttcg acgcccgctt
    13321 cccggaccag ggcgtgcccg agttcctgga gcaggtctcg ctcatcaccg agcaggacga
    13381 gtacgacgag accgagaccg gcgtgaccct gatgacgctg cacaccgcga agggcctgga
    13441 gttcgacgtg gtcttcatcg ttgggatgga ggacggcgtc ttcccgcatc accggtcgat
    13501 gaccgacccg gccgagctcg aggaggagcg ccgcctggcc tacgtcggga tcacccgggc
    13561 ccgccgccgt ctgtacctga cccacgcctg gagccgctcg ctgttcggcg cctcgtcata
    13621 caacccccct tctcgcttcc tggccgagat cccgtccgag ctggtgcacg aggtcgaggg
    13681 cgagggcagg acgcctcgcg aagagctgct tccccccccg gtccacacgg tcgaggtcgt
    13741 ggcgggcgac acggtgcggc acgatcgctt cggcgagggt gtcgtcgtag ccgtgacggg
    13801 cagggggagc gacgctgagg tgacgatcat gttcgaggac gagggcgaga agcggctgct
    13861 tctggcctac gcccccctgg agaaggtggg ctgaggcctc agtcggcctc caccacgaag
    13921 ttgggggccg cctcggccac gccggcgatc tccaggacct ccatcacggg gcgcgtgggg
    13981 ttgcgaagga tcaggtcgcc ggactcgccc agctgcaccg cgacgtcgat cagcacgcgg
    14041 agaccggtcg agtcgatgaa ggacagatcg gagaggtcga gaaccaggga gccctctccg
    14101 gctcctgcga tctcccggag cacctccgcc agctccggcg aagacgccat gtccagctcg
    14161 ccggagactc ggacgaggcg cggcgcggca cgctcgatcc gcacccgatc ggtcacgagc
    14221 gggtccctcc ctcgttcgtg tgggctggca cagtctaatc tgctcgccct gctccgtcag
    14281 gggccgcgaa cgacccgccg gctgggatcg gcgtagagga ccccatcgtg gatcctgacg
    14341 gggacgaggt cgagcgggtc ccccctgtcc ttggggaggc cgtcgagccc gtaggtggcg
    14401 ccctcgcagt ccgcgtagcc gtcctcgctc ggctcgaggg ggcagcccgc ccgctcggcc
    14461 accgcgatca cgcgcccgtc ggacaggcgc cccacgaaaa cgccgggcac cgtctcgcga
    14521 ccgtcgccga ccgggagcga ggcgacgtcg gtcactggac gccacggggg ggacggctcc
    14581 cggccccgca ggaacagcca gccggccgcc aggaggatca cgcccagcac gatcggggcg
    14641 atcacccact ggagccccag ccgtccccgg aagggcccga ggggtccgat cttctgcccc
    14701 ggcttcacgc cgggcgagcg aaagcggatc cgcggcctgg ccatgccccc atcatcccag
    14761 gacgggcggc ggccgcccgc ggccatccgc tatagttggc cgcgtctccg gaggccagag
    14821 gggaaacgca tgcccgggaa gcaccggatc ctcatcgcga agcccggcct cgacgggcac
    14881 gaccgggggg cgaaggtcgt cgcccgggcc cttcgcgacg ccgggtacga ggtgatctac
    14941 accggcctcc accagacgcc cgagcagatc gtcgaggccg cgatccagga ggacgtcgac
    15001 gcggtgggcc tctcgatcca ctcgggcgcc cacatgaccc tctttcccaa ggtggcggcg
    15061 gccctccgcg agcggggcgc cgacgacgtg gtggtcttcg gcggcgggat catccccaag
    15121 gaggacgtct ccgccctcca ggaggcgggg atcgagcgag tgttcacgcc aggggcaccg
    15181 acgtcgtcga tcgtggagtg gctcgaggag cggttggggg ccggggagga gacctcctag
    15241 gtggacctgt acgagcatca gggcaaggac ctgttcggag cccacgacat cgccctcgtg
    15301 gacggcgaga tcgccaccgc gcccgaggag gcccggtcga tcgctgagcg ctacgggggc
    15361 gccgtggcgg tgaaggtgca ggtccaggtc gggggacgag gcaaggccgg aggtatcgcg
    15421 ctggtcaccg gcgcagacga ggccgaggag gccgcccgcc gcatgctcga ggagggtttc
    15481 cgcggcacgc gggtcacccg cgtcctcgtg gagcggctcg tcgacatcga gacgcagtac
    15541 tacatggcga tcacgcttga tcggtcgtct cgccggtacc tggcgatgac ctcgaacgag
    15601 ggggggatgg agatcgagga catcgccaag gagaagcccg acgcgatccg ccgctccgag
    15661 atcgatcccg tgctcgggct gatgccgtac cagacgcgct acctcgtggg gcacttccct
    15721 gaggccgcga ggaaggggag cgccgacatc ctcggcaagc tctacggggt gctgaccgcc
    15781 gcggacgcca cgctcgtgga ggtcaacccg ctggccctcc tcacggacgg ggccgtggtg
    15841 gccctcgacg ccaaggtgac cctcgacgac aacgcgctgt tccgccaccc ggacctcgcg
    15901 gagctggcca aggacttcgt cctcgacccc gtcgaggcca gggccaagga gctcggcctc
    15961 cagtacgtga agctcgacgg agaggtgggg atcatcggga acggcgccgg cctggtgatg
    16021 tccactctcg acgtggtgga ccaggccggg gggtccgcgg ccaacttcct ggacgcgggc
    16081 ggtggggccg gggcggacgt gatggccgcg tcgctggagg tgatcctctc ggaccctgcc
    16141 gtgcgctcgg ttctggtgaa catcttcggg gggatcaccc ggggcgaccg cgtggcccag
    16201 ggcatcctcg aggccctggg gcgcatcgag gtcgaggtcc ccatcgtggt ccgcctcgac
    16261 gggacgaacg ccgaggaggg ccgcaggatg ctggcggagg cggagcacga ccggatcatc
    16321 cccgcggcca cgatgctgga ggcggccgag aaggccgtcg cggcggcgaa ggagagctcc
    16381 tgatggcggt cctcgtcacc gaggacaccc ggcttgtcgt tcagggcatc accggcaagg
    16441 agggggcctt ccacaccctc cgcaaccgcg actacgggac ccccatcgtg gccggcgtca
    16501 cccccgggaa ggggggcgat tcggtggacg gcatccccgt cttcgacagc gtccgggagg
    16561 cggtcgagga gaccggggcc aacacctcgc tcacattcgt cccggcccgg ttcgcggccg
    16621 acgcgatcct ggaggccgcc gacgcgggcg tcgacctcgt cgtgtgcatc accgagggca
    16681 tcccggtcca ggacatggcc agggtctgga actacatgct ggggaaggac tgccggctgg
    16741 tggggccgaa ctgccccggc gtcatctcgc cggggaaggc gaacatcggg atcatccccc
    16801 acgagatcac gaaggcgggg ggcgtggggc tcgtctcccg ctcgggaacg ctgacctacc
    16861 agatcatgaa cgagctgacc cagcagggca tcggccagtc cacctgcatc ggcatcggcg
    16921 gcgaccccat catcggcacg gggttcgtgg acgcgctgga gctcttcgag gccgaccccg
    16981 agaccgaggc catcgtcgtg atcggcgaga tcgggggcga cgacgaggag aaggccgccg
    17041 ctcacatcgc ggagcacgtc tcgaagcccg tcgtgggcta cgtggcgggg ttcacggccc
    17101 cgccggggaa gcggatgggg cacgcgggcg cgatcatcac cggctcgaag gggacggcca
    17161 aggccaaggc cgaggcgttc gaggaggcgg gggtgccggt ggggaggaac cccaccgagg
    17221 tggcccagat cgtggcctcg atccgaagct gacttcccac cacccgtccg ggcatcgggg
    17281 catactgatg ggcggcccgc cgacctgagg gggacatcgt gacctgtccg tcgtgcaacc
    17341 agcccgtacc cgagggagct cgcttctgcc ccaactgcgg agccgcccag cccgtggcgc
    17401 aggccccggc cgagcccgtc gactcgcagg agggcccggg gggacccccg gcgccctcga
    17461 ccgagccccc gcccccgcct ccccccaccg agcgtccgcc cggtgcggag caggtggctc
    17521 cgtctccacc cccacctacc cccccgccca gcgagacgcc cgcaccacca ccgcccgccg
    17581 gcgacggacc cgagccgatg ccccgcgcgg tgaccggaga gcccaggctc agcgccggga
    17641 tcatgcgcgc gctcctcctt ggggccctgg tattcctcgt gatgctgctc ctgtccgccg
    17701 tgatcgcgtt cgccgccttc ttcacgagcg atgccgacgt ggctgaggtg gcgaaggtcg
    17761 gtggcgtcat cttcttctcc ttccatcacg tgggcctgcg cacggagctc cccacgttcg
    17821 atccgtcggc cgtccccgga gcgggggcca cccagggcgg cctgccgctg ggcccgatcg
    17881 atctctcgtt cacgatctgg gcggccctcc tgctgggaac cctgctggcg ctcgtcctgc
    17941 tgtaccgggc gggccgtcgc gccgccgagg agggtggcgg ctcgaccgga gcgcggatcc
    18001 tccagggggc gttcgtggca gtcccctacg cggtgctggc ggcgctgctg tcgctcgtgg
    18061 tccgattcga gctcgacctc ggtccacagc tcgcctcctt cttcgggtcc gctggcgccg
    18121 tggaggtgtc ggcgccgctt ctctcggcgc tcctgtggcc cctcgggatc gcggtcgtgg
    18181 ccgggggtct gggagggtac ggcgccgctc gtcgcgacgc cgctgcaggg acgccgggga
    18241 ggctgggggc catggtctcg ggcgcgtgga ggatgctcct ggccctcctc gtggccgggt
    18301 tcgtcgggct cctgatcctg ggggcgatca accccgacct gaccgcgtcg tacttcgaga
    18361 cggtggggag cgggcgatcg ggcctcgtcc tgctgctggc gacgatcgcc gccgtgccga
    18421 acctcgcgct gtggggcatc accctgggga tgggaggcag catcgtggcc ggcgatctgc
    18481 tgggcgcgga cgcggtgcgc atcgcctccc tgctcgagtt cccgaccgat ctggggctcc
    18541 agttggcggg cggctccctc gtggggggga cgcctcaggt ctcgaccgaa gtagcgccga
    18601 tcgagtactt cgccttcatc gtgctgaccc tgatcgcgct ggtcatcggc gggaggatgg
    18661 ccgcccggcg agcccacgtc gtctccgcgg gccagggcgc gctcatcggc gccggctcgg
    18721 gggtgctctt cgccatcgcc ttcttcgggg ccctggccct cgcggggatc acggtgaaca
    18781 cctcggtcca ggctggtcag gacgggctcg tccggatggg tcccgggctg ctgctcggtg
    18841 gactcttggg cctggcctgg ggcgtggtgg ccggggccat cgggggggcc acggggtctg
    18901 gggccgaggc gcccttcccc atcgatcggc gcccggcgcc ggcgcccgcg cccccggggg
    18961 aggggccgga ggccacgcag gagctgccgc agcagccggc gacggggacc gggttcgagg
    19021 gcctcgcggc tccgcgtgcg gccgtgcggc ccgagcccga gcccgagcct gaggccggac
    19081 cggcgccgga gccggagcag ggggccctgg ccgaggagga ggaagcgagc gcgccgctcc
    19141 aggctcccac ggagcacgcc tccggtgagc cccccgcctt cgagttcacg accccgcagg
    19201 cgcagtggcg ggcgcccgag cccgaacctc cagccgaggg cgaggcgccc gccgggccgg
    19261 aggagccggg gggcggctcg gctcccgagc ccgatcagga ggagccgacg ggtgagcagg
    19321 ggccgccgca gacgcccggg tggacggctc cggagcggga cgccgagccc ccctccgagg
    19381 ccccgccgtg gagcgacccg gcggacttcg agccgcggcc gtggccgggc gacgagcccg
    19441 agcccggacc ctccgaggag gtggatgacg acgaggggga agaggagggg cccgccccgg
    19501 cctgagccgg gcatgcgaga gggcccgggg cctgtggccc cgggccctct cgcatgccct
    19561 ctctacgctg cggggggagc cggtgggggc atccccatcg ccttgctctc attgaaccgg
    19621 acccaggccc cgtaggccat ggccaggagc acgatcagcc cgacccatgc gccccaggcg
    19681 atcccgtagc cgctccgggt gagcgcgatg atgaagcgga tcagcccgaa caggaccgtg
    19741 gcggcggcga gcccggcgct gatcagcgcc tcggacacgt tcaggttcag gttgacgccg
    19801 gccatcagca tgagctccca gaccaggagc aggatggcga ggatcccggc gaggaccccg
    19861 atcccgttcc agccggagat gttcacgccc gggaggtcga accccggcag gccgcccaca
    19921 tcgaagctct cccacgggaa gaagagcgtg acgaggaaga cgaggccggt gatcaagagg
    19981 gtcttctgac ccatcgtgag cctcgagatg tcgaacccct gctgcgccat gcggtctcct
    20041 ttctcggtct cccccgggac ggtgggttct ctcgcgtgat tctcgtgacc cggctgaggc
    20101 gagtcaagga cgctgccaga acgccgcggt ggcccgtacc gcgcccgtca gcgtggcggc
    20161 cacggccagg ccgaggacga cgatcggcac cggcccgcgc gtcgacagct cgggggcggg
    20221 gagggcgagg atcgtcgcca ggacggccag ggctcccagc gcgatgtgga cggcgcggag
    20281 tcggccctgc cgcccgcgga gcgatgccac cgtggccagg aggagcagca cgcctgccac
    20341 gaacggccac ggctcgggga ccggcgtcca cgccgagagg aaccctgcgt gcgacccctg
    20401 ccgatcccag ggcaggagcg agacgaggac ggcggcgagg aagaggaggc cggcggggag
    20461 gtcccggaac cggaccgatc gaggtggagg ggccgcctcg atcggctcgc ccagctcctc
    20521 gcccacgcag tccatgcagc gcacctcccc ccggacgggg acggcgcagc ccaggcacgc
    20581 ggcccgaccg caccttccgc aggtctcccg ggccgggacg tccgggtgga cggagcaagc
    20641 gctggcgctc acgcccatat ggtagtttgc gcccggtgaa cgctcggctc gcggtcctgg
    20701 cgtcgggctc cgggaccaac ctccaggcgc tgctggacga tccggtcgtt ggtccgcggg
    20761 tgtccctcgt cgtgagcgac cggggcgacg cgggcgcgct cgaccgggcc gcggccgccg
    20821 gtgtgaaggc cgtgcacctc gatcccgcga tccacgacga gcggaccgcc tacgacgccg
    20881 cgctccagga cctcctcgtc tccgagggca tcgacctcgt gtgcctcgcc ggcttcatgc
    20941 gcgtcctcgg ccccaccctg gtccggtcct tcgaggggcg gatgctcaac gtccatccct
    21001 ccctgctccc ggccttcccc ggagcccacg ccccgcgcga tgcgctgaag tggggagcga
    21061 aggtcaccgg cgtgaccgtg cacgtcgtgg acgaggaggt ggaccacggg ccgatcgtcc
    21121 tccaggagcc ggtgcccgtc cgcgaggacg acgaccccga gtcgctccac gcgcgcatca
    21181 aggacgtcga gcaccgcgtc tacccgctgg cggtgcggtg gtggctcgag gggcgggtcc
    21241 gggtcgaggg gcgccgcgtg cgcatcgagg gcgaggcttg agcgacctcc tccccgttcg
    21301 gcgagcgctc gtctcggtgg ccgacaaaga ggggctgatc gcgctgggcc gcggcctcgt
    21361 cgaggagggc gtgacgatcg tctccaccgg ctccaccgcc cgggccctcc aggaagaggg
    21421 cgtgccggtg acctccgtcg ccgaggtgac cgggttcccg gagatgctcg ggggccgggt
    21481 gaagaccctc cacccggcga tccacgcggg catcctggcg gacaagcggg acgaggagca
    21541 cctgcgccag ctcgaggagg cgggcatcaa gccgttcgac ctcgtcgtcg tcaacctcta
    21601 cccgttcgag cggacggtgc gctcgggggc cgaccgggac gaggtcgtgg agcagatcga
    21661 catcggaggc cccacactgg tgcgggccgc cgccaagaac ttcggctcga tcggcgtggt
    21721 cgtggacccc gagcgctacg gggagctgct ggcggcggtc gagggcgggg ggcttcccga
    21781 gcggctgcgc gcctcgctgg cggaggaggc cttccggcac gtggccctct acgacgccgc
    21841 gatcgccgcg tggttcgggt cctgggccgg ggaaggagag ctgctgcccc gtgtcctggc
    21901 cccgggcttc gagctcgcgt ccccgctccg ctacggcgag aacccgcatc agcgggccgg
    21961 cctctaccgg gcggcgctct cggacggtcc gctgggcgga gccgaggtgc tccagggcaa
    22021 ggagatgtcc tacaacaact ggctcgacgc ggaggcggcc cgggcgctgg caagctcgtt
    22081 cgaggagccc gcctgcgtca tcgtgaagca tcacaacccc tgcggcgcgg cggccaccgg
    22141 ggacctcgcg gactgctacc gggacgcgct cgcgtccgat cccgtgtccg cgttcggggg
    22201 catcgtggcc ttcaaccgcg aggtggacga ggccgccgcg cgcgcgatgg aggaggtctt
    22261 caccgaagtc gtggtggccc ccggcttctc gtcggaggca cgccagctcc tcgcggggcg
    22321 caagagcctg cgcgtcgtgc tcgcgccgcc ccccgcgccg ggggggctgg agctccggcc
    22381 gatcgaaggc ggggcgctgg tccaggacgc cgacgcggtc cgcgagctca gggatgcgat
    22441 gcgggtcgcg acgcgtcggt ccccgaccga ggaggagtgg gaagggctcc tgttcgcctg
    22501 gcgcgtggcc gcccgggtga agtcgaacgc gatcgtcctg gcgcggggtc gggccaccct
    22561 gggggtgggc gcggggcaga tgagccgcgt ggacagcgtg gagctcgcgc gccggaaggc
    22621 cggcgaccgc gcgatgggct cggtcctggc ctccgacgcc ttcttcccgt tccgggacgg
    22681 gatcgacgcg gccgcagcgg caggggtgac ggccgcgatc cagcccgggg gatcggtccg
    22741 cgacgaggag gtcgtggcgg cggccgacga gcacggtatg gcgatggtgc tcaccggggt
    22801 ccggcatttc cgccattagg agagcaagga gggcggcctc cgctcaactc gcccgccccc
    22861 gtcgaagggg atatgctcgc ccgcgtacgc ccagggcccc gtgcctcggg gccctcggcc
    22921 cgccggaaga agggaggcag agatgcgaag gggcaccttg gcgaggctcg gcgcgctcgt
    22981 agccgtcgtc gcgctactcg cggccgcctg tcagagggga gaggacgccc ccgaggccac
    23041 cgacgaggag gagccgcagg ctcagcagga gggaggcctg ctgcaggaga tcagggagcg
    23101 ggggatgctc cggtgcggcg tgaacaacac cgtgcccggg ttcggcttcg agacctcggg
    23161 gggtgagatc gaggggttcg acgtcgattt ctgcaccgcg atcgccgcgg ccgtgttcaa
    23221 cacccccgat gtggagggga tgatcgagct cttcccggtc gacgcggagg agcggttcaa
    23281 cgccctccgc gcgggcgagt acgacgtcct cgtgcgcaac acgacctgga cctcgagccg
    23341 cgacggctcc gagggcgtgg ccttcgccca cgtcaacttc tacgacggcc aggcgatgat
    23401 ggtccgcgag ggtgagttca acgcggtgga cgagatggac gggacctccg tctgcgtcac
    23461 gaccgggacc acgaccgagc tcaacctcgc cgactacgcg gcgcaggtgg gcgtggacat
    23521 cgagccggtg acgttcgccg agaacccgca gctgcaggag gccttcatct ccggtcgctg
    23581 cgacggctgg acctccgacc ggtcgcagct ggccgggatc cgctcggcat ggccggacga
    23641 ggagggcggc cccgagtcgc tcgtcatcct cgacgacatc atgtccaagg agccgctggc
    23701 tccgggagtc ctcgatggcg accaggagtg gtacgacgcc gtgaactggg tcgtgaacgg
    23761 gctcatcctc gcggaggagc tcggggtgga cagctccaac gtcgagcagc aggccgagaa
    23821 cccggacgac ccccagatcg cggcgctgct cggggtgtcg ttcgaggccg agaccgagac
    23881 gatcacggtc gaaagcggtc tggggctgga ggacgacttc atgcaggccg tgctgcaggc
    23941 cgtggggaac tacggcgaga tcttcgaccg tcacatcggg ccggattcgc cgctcgggct
    24001 cgaccgaggc gtgaacgcgc tctggaccga cggcgggatc cagtacgcga tgccgttcag
    24061 gtagaccgat gatcggcggg gcgggggggg ggg
//
LOCUS       DASUTZ010000110        11981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_157755, whole genome shotgun sequence.
ACCESSION   DASUTZ010000110 DASUTZ010000000
VERSION     DASUTZ010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11981
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157755"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1880
                     /locus_tag="VF097_05025"
     CDS             <1..1880
                     /locus_tag="VF097_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX6262193.1"
                     /translation="ADVWVEGYYETGMQDQAPLGPEAGLAVPAEDGGVDLYVATQWLH
                     VDHRQIAGCLGLPDEKVRLHLAGVGGAFGSREDVSMQIHASMLALRTGRPVKMSYGRE
                     ESFFGHVHRHPARIWMRHGATSEGRLVSVRARIVIDGGAYSSSSAAVIANATTFAAGP
                     YEVPNALLEGTCVYTNNPPCGAMRGFGAVQAAFGYESQMDRLAAALAMDPVELRERNA
                     LSTGSVLPTGQVIRGSAPVREVIERCARIPMPPEPDGIREPVTLPGGANNVGRGEGLR
                     RGVGFAVGYKNIAYSGGFDDSAAARVRLSAGPDGPVAEVHSASAEVGQGVHTILAQIA
                     GTELGVDRVELLTPDTGVGSAGSSSASRQTMMSGGAVQLACRAVRETLLERVRLARPE
                     VGDAPLTLEEGRVLADGLPVGDLVEFLVDPIEESRVYRHRPTEPFDPDGQADFHVSFA
                     FAAARAVVEVDEDLGLVRVVEVAAAQDVGRALNPQSVRGQIEGGIAQGLGLALMEEIQ
                     LEEGRILNASFTDYLIPTTLDMPPVVSELVEEPEPGVPFGAKGVGEPSTIVATAAVAA
                     ALRDAVGRALNRVPVRPDDLVGIALPVSTRGWPRVPDVPGQEPVPVYAGVPATELAQK
                     A"
     gene            1894..2895
                     /locus_tag="VF097_05030"
     CDS             1894..2895
                     /locus_tag="VF097_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX6262194.1"
                     /translation="MEREALTGRDYIETLDWSVEELEEALAVSAELKERYKRGEPHRL
                     LADKTLFMLFLDKSTRTRNAFEAGMTQLGGHAHYLTAEATQVSHGESPKDMGIILSSY
                     GHGVAIRHDLIPYECNAYIREVARWAEVPVLNLQCDVDHPTQTLADLMTLREKRGADL
                     RGMRVAVSWAYAPSYAKPLSVPQGLAALLPRFGIDVVLAHPAGWELMPELMARAEKAA
                     SEGGGSITVTNDMEEAFEGADAVYPKSWGRLDLFTQEQEALAASEPHRGWICDARRMG
                     LAKDDALYMHCLPADRGNEVTDEVIDGPRSVVYEEAENRLHTGKALMALTMGGFGGA"
     gene            2903..4030
                     /locus_tag="VF097_05035"
     CDS             2903..4030
                     /locus_tag="VF097_05035"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HEX6262195.1"
                     /translation="MISSDRILPNPLRGAVPAPTPGPDPLAFHRRLPGYEVTPLVEAP
                     SVARRLGVGSVWVKDESHRLGLPSFKILGASWATYRTLVDRLGRDVEPWSDVAELSER
                     LSPLRPLTLAAATDGNHGRAVARMAALLGLGARIFVPAGTARARIEAIEGEGAEVEVV
                     AGTYDDAVRRAAGEASPKCAVISDMAVTDGDASPRLVIEGYSTIFRELDEQLPEPVDL
                     FAVQVGVGALAAAAVLHYRGSEARLVGVEPEEAACVMASIRAGEIVSLPGEQHSIMAG
                     LNCGTPSAVAWPIVSAGIDLFVAVPDDQVPEAVRLMAEAGIVSGETGAAGAAGLLEAL
                     TGPDAEATRELLRVDGSTRVLLLSTEGATDPESYRESLARP"
     gene            4193..4807
                     /locus_tag="VF097_05040"
     CDS             4193..4807
                     /locus_tag="VF097_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6262196.1"
                     /translation="MATLQVTITPMAPEHLAETGRVLGAAFASSPLERAIRGPIDGRQ
                     RRGLVRAYTTVCRISGHVSVAWRDDRIVGAIRWVASPRCQLRLREKVRAAPTAVGAFG
                     RNLPKAMRWVREWSRRDPSEPHVHLGPIGVAPALQGQGIGSRMLSLYCERLDRVGDAG
                     YLETDKIENVRLYERFGFEVREQGVLHGVSNWFMWREPVQLSRV"
     gene            5100..5558
                     /locus_tag="VF097_05045"
     CDS             5100..5558
                     /locus_tag="VF097_05045"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX6262197.1"
                     /translation="MKKASFAVLGYVTPTGEPRSSGVVYKTLGRRLYVATAVDSWKAR
                     HIAAGGRVSVTVPVRRGGLLSLVVPIPPATVSFHGTALVHPAGAPEVASILEELGSLL
                     PRERRDSAAIIEIEPEGTFVTYGVGVSLTKMRDPAAARARTPVSPGGVPS"
     gene            5672..6340
                     /locus_tag="VF097_05050"
     CDS             5672..6340
                     /locus_tag="VF097_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF domain-containing protein"
                     /protein_id="HEX6262198.1"
                     /translation="MRPIRLWIGAALVVLGVLAVLDATGVLESTGVVERWWPIAVIGT
                     GVLGMVLRRRLTLGMLVITAIGFALLADAQDWTIEDVLGPALLVIIGLVVLAPFWSRG
                     RMTASDTEGESPFALFGAAKVKDHSEHLQHAEASALFGGATLDLREAHIEDEATVEAT
                     AIFGGVDVLVPKDVRVSLGGLPIFGGLEDKTTGDGELPKDAPHLNVRATAIFGGVDVA
                     YEPD"
     gene            6531..7016
                     /locus_tag="VF097_05055"
     CDS             6531..7016
                     /locus_tag="VF097_05055"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEX6262199.1"
                     /translation="MKKGGVMELSEWLAFIHILTAMFWVGAGVVLNLATSRVAASGDD
                     ERVVQFTRNVEFLGNWLIGPSAVAVVGTGIWITAIEEWVAFSQLWIILALVGVAVSML
                     LGMGYFGPEGKRLGRLAEERGASDPELKTRTRRLLLLSRLDILVLVFLTWDMVFKPGL
                     E"
     gene            complement(7056..7592)
                     /locus_tag="VF097_05060"
     CDS             complement(7056..7592)
                     /locus_tag="VF097_05060"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6262200.1"
                     /translation="MAHLRLETERLLLRPLSLRDLGDMASLLGDAEALTFWGDPLDRE
                     GARDWIQRNLHRYETDGVGRCAVVLRATGELVGDCGLIRTLVEGSRETELGWIVRRSH
                     WGKGIATEAGEAWRDHAFKNLGIDRIVSMISEANAASRRVAEKLGMTIEREAMWGGLP
                     HLMYSLRPGVLDRRTDQR"
     gene            complement(7633..7866)
                     /locus_tag="VF097_05065"
     CDS             complement(7633..7866)
                     /locus_tag="VF097_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX6262201.1"
                     /translation="MTMMMGGGLLVLVAVVALAIYVARGGFGLRPPDDQREHRPEARE
                     ILEERFARGEISEDEFEQRRQALERTSSSMRGG"
     gene            complement(8036..8644)
                     /locus_tag="VF097_05070"
     CDS             complement(8036..8644)
                     /locus_tag="VF097_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262202.1"
                     /translation="MALLVLLPLAFYLTTPSQNEFALVAGGTGMAWAVAGGALFLTLA
                     SRRIDSRLVLAGYRPREVLIGRLLLLEGLALVLVALFSALMVAISSPPEPGALVLAVA
                     LSGLVAVPLGLAVAAVFPRELEGALTLIAVVGIEMGVPVDSGLAPFLPLWGPLKLVEV
                     ARGAADGLALPTLHALASMVVLLVISAVLWWRRVRVTRHAAL"
     gene            complement(8710..9378)
                     /locus_tag="VF097_05075"
     CDS             complement(8710..9378)
                     /locus_tag="VF097_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018569834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6262203.1"
                     /translation="MAPRSSLRGDRPVALAANGISKRFGRKAVLEDVSLALRPGEAVA
                     LVGENGAGKTTLLRICAGLLRADSGTVHVPGRIGYCPQDAGVLDLLTADEHMVLFGRA
                     AGLSRESALREGRRNLEWFGFPRQERAVAKDLSGGTRQKLNLALALLGDPEVLLLDEP
                     YQGFDRGTYVNFWDHVDDWRSAGKAVLVVTHMMAELTRVDRVVELPVLTTGGREPEPA
                     GGPS"
     gene            9608..10504
                     /locus_tag="VF097_05080"
     CDS             9608..10504
                     /locus_tag="VF097_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6262204.1"
                     /translation="MLQIDGLSKRYGDVVALDGASFSVASGRIVGFLGPNGAGKTTTM
                     RAIFGLVRPDGGEVRWKGTAVGPAERARFGYMPEERGLYPKMRVGEQLIYFGRLSGMS
                     SAAATDAAETWLARLGLGERVGARLEELSHGNQQRIQLAAALIHDPELAVLDEPFGGL
                     DPLGVESLGEMLAQAAALGTAVLFSSHQLDLVEHVCQDVVIIDRGSVVLAGAVDELKA
                     ASARRSLEIRVDGEQWVPVLPSGTVTSKQDGGLRVLVDAAVDLDEILASARRAGEITT
                     FSFEPPSLTDLFLEAVSEGRGT"
     gene            10531..11688
                     /locus_tag="VF097_05085"
     CDS             10531..11688
                     /locus_tag="VF097_05085"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6262205.1"
                     /translation="MARREWDQRVRSLSFRLSTLLSILIVVALIAVPEMYGGGSEPTH
                     TVGLVGDSGAELPASLRSAGEEMGLSVRVREFADESSGRAALRSEDISALLVDQRDLV
                     WRAEVDQGLQAAVISAVQALDRREAIAELGLTSEEAGRLIGPPDLRSTSLEPAAAERS
                     ARRDLGFVAVVMLFMAIAFYGGFVLVGVVEEKSSRVVEVLLARIRPAQLLGGKILGIG
                     LVGLAQFALVVGSALVAVSLSGNTLLPSTTPSTLGWILFWFVTGYAFYSVLFATAGSL
                     ISRQEEAQSVQFPVTAFLLVGYVVAFQAAAAPEGVAALVGSLLPPTAPMVMTVLMAQG
                     GVAWWHVALSAALLMATTYGMVLLAGRLYSGAVLRLGRRVRLREAWRGAEG"
     gene            complement(11741..>11981)
                     /locus_tag="VF097_05090"
     CDS             complement(11741..>11981)
                     /locus_tag="VF097_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262206.1"
                     /translation="PKVAVPIRIGGGASDITVIRPEGVPIRVRTGGGSSNLTIDDLRF
                     ASAGKTDWQSPDYEGAEARYDIEIGAGTSAFTVRT"
BASE COUNT         1731 a         4063 c         4366 g         1821 t
ORIGIN      
        1 aggcggacgt ctgggtggag ggctactacg agacggggat gcaggaccag gcgccgctcg
       61 ggcccgaagc agggctggcc gtccccgcgg aggacggagg ggtggatctg tacgtggcca
      121 cccagtggct ccacgtcgac caccggcaga tcgcggggtg tctcgggctc cccgacgaga
      181 aggtccgtct gcacctggcg ggggtggggg gcgccttcgg gtcccgggag gacgtgagca
      241 tgcagatcca cgcgagcatg ctcgccctcc gcacggggag gcccgtcaag atgtcctacg
      301 ggcgtgagga gtccttcttc ggccacgtgc accggcaccc ggcccgcatc tggatgcgcc
      361 acggcgccac gagcgaggga cggctggtga gcgtccgggc gaggatcgtc atcgacggag
      421 gcgcctactc ctcgtcgtct gccgccgtca tcgcgaacgc gacgacgttc gcggcggggc
      481 cctacgaggt cccgaacgcc ctcctcgagg ggacctgcgt ctatacgaac aacccgccct
      541 gcggcgcgat gcggggcttc ggcgccgtcc aggcggcctt cggctacgag tcgcagatgg
      601 accggctcgc tgcggccttg gcgatggacc ccgtggagct gcgcgagcgc aacgcgctct
      661 cgaccgggtc ggtgctcccc acgggccagg tgatccgggg gtcggccccg gtccgcgagg
      721 tgatcgagcg ctgtgcccgg atcccgatgc cccccgagcc cgacggcatc cgggagcccg
      781 tcacgctccc cggcggggcc aacaacgtgg gtcggggaga gggtctgagg cggggcgtgg
      841 ggttcgccgt cggctacaag aacatcgcct acagcggagg gttcgacgac tccgcggcgg
      901 cgcgggtcag gctgtcggcg gggccggacg gtcccgtggc ggaggtgcac agcgcctccg
      961 cggaggttgg ccagggcgtg cacacgatcc tggcgcagat cgccgggacc gagctcgggg
     1021 tcgaccgcgt cgagctgctc acccccgaca cgggcgtcgg gtccgcgggg tcgagctcgg
     1081 cctctcggca gaccatgatg tcgggagggg ccgtgcagct ggcgtgccgg gccgtccggg
     1141 agacgctcct cgagcgcgtg cgcctggctc gaccggaggt gggggacgcc cccctcaccc
     1201 tcgaggaggg gagggtgctg gccgacgggc ttcccgtggg cgacctcgtc gagttcctcg
     1261 tcgatccgat cgaggagtcg cgcgtctacc gccaccgccc gaccgagccc ttcgatcccg
     1321 atggtcaggc cgacttccac gtctccttcg ccttcgccgc cgcgcgggcg gtggtcgagg
     1381 tggacgagga tctgggcctc gtgcgcgtgg tcgaggtggc ggcggcccag gacgtcggtc
     1441 gggcgctcaa cccgcagagc gttcgtgggc agatcgaggg cggcatcgcg cagggcctcg
     1501 gcctcgcgct gatggaggag atccagctgg aggagggtcg gatcctgaac gcctcgttca
     1561 ccgactacct gatccccacg acgctggaca tgcccccggt ggtctccgag ctggtcgagg
     1621 agccggagcc gggcgtcccg ttcggcgcca agggcgtggg cgagccctcc acgatcgtgg
     1681 ccaccgcggc cgtcgctgcc gcgcttcggg acgcggtggg aagggcgctg aaccgggtgc
     1741 ccgtgcgccc cgacgacctg gtcgggatcg cgctcccggt ttccacccgc gggtggccgc
     1801 gcgtcccgga cgtccccggc caggagcccg tccccgtgta cgcgggcgtg ccggccaccg
     1861 agctcgccca gaaggcctga ggaggagaga ggcgtggagc gcgaagccct gacggggcgg
     1921 gactacatcg agacgctgga ctggtcggtg gaggagctcg aggaagccct cgccgtctcg
     1981 gccgagctga aggagcggta caagcgaggc gagccccacc ggctgctcgc cgacaagacg
     2041 ctgttcatgc tcttcctcga caagtcgacg cgcacgagaa acgcgttcga ggccgggatg
     2101 acccagctgg gggggcacgc gcattacctc accgccgagg cgacgcaggt ctcccacggc
     2161 gagagcccga aggacatggg gatcatcctc tcgtcctacg ggcacggggt cgcgatccgc
     2221 cacgacctca tcccctacga gtgcaacgca tacatccgcg aggtggcccg gtgggcggag
     2281 gtgccggtgc tcaacctcca gtgcgacgtg gatcacccga cgcagaccct ggccgacctc
     2341 atgaccctcc gggagaagcg cggggccgac ctgcggggga tgcgggtcgc cgtctcgtgg
     2401 gcctacgccc cctcgtacgc caagccgctc tcggtgcccc agggcttggc cgccctcctg
     2461 cctcggttcg ggatcgacgt cgtgctggcg caccctgccg gctgggagct catgccggag
     2521 ctcatggcac gggccgagaa ggccgcgtcc gaaggaggcg gatccatcac ggtcacgaac
     2581 gacatggagg aagctttcga gggagccgat gcggtctatc cgaagtcctg ggggcggctc
     2641 gacctcttca cccaggagca ggaggcgctg gccgcgtcgg agccgcaccg cggctggatc
     2701 tgcgacgcgc ggcggatggg gctggccaag gacgacgccc tctacatgca ctgcctcccc
     2761 gccgatcggg ggaacgaggt gaccgacgag gtgatcgacg gcccgcggtc ggtcgtctac
     2821 gaggaggccg agaaccgcct ccacacgggg aaggcgctga tggcgctcac gatgggcggg
     2881 ttcggcgggg cgtgagctgc ggatgatctc gtccgatcgg atcctgccga accccctccg
     2941 cggcgccgtc ccggcgccga ccccggggcc cgacccgctg gcgttccacc ggaggctgcc
     3001 cgggtacgag gtcacccccc tggtggaggc cccctcggtg gcccggcgtc tcggcgtcgg
     3061 gtccgtctgg gtgaaggacg agtcccaccg cctcggtctc ccgtcgttca agatcctcgg
     3121 cgcgtcctgg gccacgtacc gcacgctcgt ggatcggctg ggccgggacg tggagccgtg
     3181 gtcggatgtc gcggagctct cggagcgcct ctccccgctc cggcctctga ccctcgcggc
     3241 ggccaccgac ggcaaccacg gccgcgcggt ggcgcgcatg gccgcgctgc tgggtctcgg
     3301 ggcccgcatc ttcgtccccg ccgggaccgc ccgcgcccgg atcgaggcca tcgagggcga
     3361 gggggccgag gtggaggtgg tggcggggac gtacgacgac gcggtccggc gggcggcggg
     3421 cgaggcgtcc cctaagtgcg cggtgatctc ggacatggcc gtgaccgacg gcgacgcgtc
     3481 gccgcgtctg gtgatcgagg gatactcgac gatcttccgg gagctggacg agcagctccc
     3541 cgagcccgtc gacctcttcg cggtgcaggt gggcgtcggg gctctcgcgg cggcggccgt
     3601 cctccactac cgggggtccg aagcgcgcct cgtcggggtg gagccggagg aggccgcatg
     3661 cgtgatggcc tcgatccgcg cgggcgagat cgtctccctc cccggcgagc agcactcgat
     3721 catggccggg ctcaactgcg gcacgccctc cgccgtggcc tggcccatcg tctccgccgg
     3781 catcgacctg ttcgtggcgg tccccgacga ccaggtcccg gaggccgtgc ggctgatggc
     3841 cgaggccggg atcgtctccg gcgagacggg cgcggcgggc gcggccggcc tgctcgaggc
     3901 gctgaccggt cccgacgcgg aggcgacccg cgagctcctc cgggtggatg ggtccacccg
     3961 cgtcctcctg ctctcgacgg agggcgcgac cgaccccgag tcgtaccggg agagcctcgc
     4021 ccggccctga ccggtcccgc tgccgagtga gcgggcgggc cgccggctcg ggacgtcatt
     4081 gccgcgcggt cgggctctcg ccgcatcggt cccgtgtgaa gggggccgga cggccctggt
     4141 ccacccctgc cgtgcgcctg acgcttgtcg aacgcgaagc aggagacatg ccatggccac
     4201 gctccaggtc acgatcacgc ccatggcccc agagcacctc gccgagacgg ggcgagtgtt
     4261 gggcgccgcc ttcgcctcca gcccactcga gcgggccatc cgtggcccga tcgacggacg
     4321 gcagcgccgg ggtctcgttc gtgcctacac gacggtctgt cgcatctcgg gacacgtctc
     4381 ggtcgcttgg cgggacgacc ggatcgtggg cgcgatccga tgggtggcat ctcctcggtg
     4441 ccagctgcgt ctgcgcgaga aggtcagggc ggcgccgact gccgttgggg cgttcggccg
     4501 caacctgccg aaggcgatga gatgggtgcg ggagtggtcc aggcgggatc cgtcggagcc
     4561 gcacgtccac cttggaccga tcggtgtggc cccggccctg cagggacagg ggatcgggtc
     4621 gcgcatgctc tccctctact gcgagcgcct cgaccgcgtc ggtgacgcgg gctatctgga
     4681 gaccgacaag atcgagaacg ttcgtctgta cgaacggttc gggttcgagg tgcgggagca
     4741 gggcgtgctc cacggggtgt cgaactggtt catgtggcga gagccggtgc agctctcgcg
     4801 cgtctagcgc cgatccggtg cggggcctca gcccggtctt ccgaaggtcc caggcaacgg
     4861 aggtcgccgg tccctatacg tcgtcctggt tccgaggcag ggttgcaggc ggaggtggag
     4921 cgatgacgtc agaggtgcag gaacgagcga acgtcgagcc cgcacccgcc gacgaggagg
     4981 cgatccactg aggcaggttc ggaaggggga gcatcatgga gacgatccag cagagagaga
     5041 cggacgtagc tcgtatcccc gtcgcgaccc gacgcacgag cgatgagatc tggcgaagcg
     5101 tgaagaaggc gtcgttcgcg gtgctcggct acgtcacgcc gacgggggag ccccgatcga
     5161 gcggcgtggt ctacaagacc ctgggacggc gtctctacgt cgcgaccgcc gtggacagct
     5221 ggaaggcgcg gcacatcgcg gccggcgggc gggtctcggt gacggtgccg gtgcgacgag
     5281 gggggttgct ctccctcgtc gtgcccatcc ctccggccac cgtcagcttt cacggaaccg
     5341 cgctcgtgca ccccgcgggg gcgcctgagg tcgcctcgat cctcgaggag ctgggctccc
     5401 tcctcccgcg ggagcgacgg gactccgcgg ccatcatcga gatcgagccc gaggggacgt
     5461 tcgtcaccta cggcgtgggg gtctcgctga cgaagatgcg tgatccggcg gcggcccgtg
     5521 cgcgtacacc cgtgagcccg ggaggcgtac cgagctgacc agcggtcctg gatcaggggg
     5581 caagaggact ccaggagagg gggtcgtggg cctctagtcc gagcctccca cgggtgacac
     5641 cgtgtgggtg cggcaaggga aggaggtcga catgagaccc atacgattgt ggatcggagc
     5701 cgcgctggtc gtgcttgggg tgctcgccgt cctggacgcc acgggcgtgc tcgagtcgac
     5761 cggggtcgtg gagcgctggt ggccgatcgc ggtcatcggg acgggcgtcc tggggatggt
     5821 ccttcggcgg cgtctgaccc tcggcatgct cgtcatcacg gcgatcggct tcgccctgct
     5881 cgcggacgcg caggactgga ccatcgagga cgtgctcggc ccagcgctgt tggtcatcat
     5941 cgggctcgtc gtcctggcgc ccttctggag ccgaggacgg atgacggcct cggacaccga
     6001 gggagagagc ccattcgcgc tgttcggggc ggccaaggtc aaggaccact ccgagcacct
     6061 gcagcacgcg gaggcgtcgg cgctgttcgg aggggccacg ctcgatctcc gggaggcgca
     6121 catcgaggat gaggccacgg tcgaagcgac cgcgatcttc gggggcgtcg acgtcctggt
     6181 ccccaaggac gttcgcgttt ctctgggcgg ccttccgatc ttcggaggac tcgaggacaa
     6241 gacgaccggc gacggggagc tgccgaagga cgcgcctcat ctgaacgtgc gggccacggc
     6301 catcttcggc ggggtggacg tggcctacga gcccgactga gggcccgatg agcggcggcc
     6361 gacgcggcca agcgcctcgg cccgatgccc gacggggctc gccaccgagg cgcttggccg
     6421 cgtccagcag cttcgtggac ctagctggct acacgcgcat cacggaagag cgcggcgatg
     6481 aggtcgccgc acagcttgac actctcttca tctacgaacg aggatcgctg atgaagaaag
     6541 gtggcgtcat ggaattgagc gagtggctgg cgttcatcca tatcctcacg gcgatgttct
     6601 gggtcggagc cggggtcgtc ctcaacctgg caacgtcgcg tgtggcggcg agcggagacg
     6661 acgagcgcgt cgtccagttc acgcgcaacg tggagttcct cggcaactgg ctgatcggtc
     6721 cctccgcggt ggcggttgtc ggcaccggga tctggatcac agccatcgag gagtgggtcg
     6781 cattctcgca gctgtggatc atcctggcgc tcgtaggggt ggcagtgtcg atgctgctcg
     6841 ggatgggcta cttcggaccg gagggcaagc ggctcggccg cctggcggag gagcgaggag
     6901 catccgatcc ggaattgaag acgaggaccc gccgcctgct tctcctctct cggctcgaca
     6961 tcctggtcct cgtgttcctg acctgggaca tggtgttcaa gcccggactc gagtgaccgg
     7021 cggcacccag agtctgaaag gcgggatcgg gtctgctagc gctggtcggt gcgtcgatcg
     7081 agaacccccg gccgaagcga atacatcaga tgcgggagtc cgccccacat cgcctcccgc
     7141 tcgatcgtca tgcccagctt ctccgcgacg cgccgcgaag ccgcgttcgc ctcgctgatc
     7201 atggagacga tccgatcgat cccgaggttc ttgaaggcat ggtcccgcca ggcctcgccc
     7261 gcttcggtgg cgatcccctt cccccaatgg ctccggcgca cgatccagcc aagttccgtc
     7321 tcccgactcc cctccaccag ggtacggatc aggccgcagt ccccgaccag ttccccggtg
     7381 gcccggagga cgaccgcgca ccggccgact ccgtcggtct cataccggtg gaggttgcgc
     7441 tggatccagt cgcgcgctcc ctcgcggtcg agaggatccc cccagaaagt cagcgcctcg
     7501 gcgtcgccga ggagcgacgc catgtcgccc aggtcgcgga ggctcagagg gcgcagcaac
     7561 agtcgttcgg tctcgagacg aagatgcgcc atgttcggac tccgcagaag tacggtaccc
     7621 gtgcgaggag cctcagccac cacgcatcga cgacgaagtg cgctcgagtg cctgcctcct
     7681 ctgctcgaac tcgtcctccg aaatctcgcc ccgggcgaaa cgctcctcca ggatctcccg
     7741 agcctccggc cgatgctccc tctggtcgtc cggcggacgg aggccgaagc ctccccgggc
     7801 aacgtagatc gccagtgcca cgaccgctac gagaaccagg agtcctcctc ccatcatcat
     7861 cgtcatcacc tcccgtcgag tcagtgacgc cacctcagac gctcagtcaa cctgttcggg
     7921 ccgagctcgc gtaggggcaa gagtcccgcc gccggttctc gggatcccgt ccgattggtc
     7981 gaagctcctc gccactgcga gagcgaggag gcgaggggaa cccgcggtgg cgggatcaga
     8041 gggccgcatg ccgcgtgacc cgcacgcgac gccaccacag gaccgcggag atcaccagca
     8101 gtaccaccat cgaggccagc gcgtggagcg tggggagggc caggccgtcg gccgcgccgc
     8161 gcgccacctc caccagcttg agaggccccc acaggggaag gaacggtgcc agtccggaat
     8221 ccactggcac gcccatctcg atcccgacga cggcgatgag cgtgagggcg ccttccagct
     8281 cccgagggaa caccgccgcc acggcgagcc ccagaggtac ggcgaccagt ccgctcaggg
     8341 cgacggcaag gacgagcgcc cccggttcgg gaggcgagga gatggcgacc atcagggcgc
     8401 tgaacagagc gacgaggacg agggcgaggc cctccagcag cagcagccgt ccgatcagga
     8461 cctcgcgcgg acggtagccg gccaggacca gccgcgagtc gatgcggcgc gaggcgagtg
     8521 tgaggaagag cgccccaccc gccacggccc acgccatccc agtcccgccg gccaccaggg
     8581 cgaattcgtt ctggctcggc gtcgtcaggt agaacgccag cggcaggagc accaggaggg
     8641 ccatggcggc ccttctacga gcgagatcgc gagcttgggc ctcggctacc gcgaagatcc
     8701 gtcccgccgt catgacggcc ctccggcggg ctccggctcg cggcctccgg tggtgagcac
     8761 cggaagctcc acgacgcggt ccacccgcgt gagctcggcc atcatgtggg tgacgacgag
     8821 gaccgccttc ccggccgatc tccaatcgtc gacgtggtcc cagaagttga cgtacgtccc
     8881 ccggtcgaag ccctggtagg gctcatcgag gagcaggacc tcaggatcgc cgagcaacgc
     8941 cagggccagg ttcagcttct gacgggttcc accggacagg tccttcgcca ccgcgcgctc
     9001 ttgccgcggg aagccgaacc actcgaggtt gcgcctgccc tcccgcaggg cactctccct
     9061 ggacaggccg gccgcgcgac cgaagagcac catgtgctca tccgcggtga ggagatccag
     9121 gacgccggcg tcctgggggc agtagccgat ccttcccggc acgtgaacag tcccggagtc
     9181 cgcgcggagg agtccggcac agatgcggag cagggtggtc ttccccgcgc cgttctcccc
     9241 gacgagggcc acggcctcgc ctggccggag agcgaggctc acgtcctcca ggacggcctt
     9301 ccgcccgaac cgcttgctga tgccgttcgc ggccagtgca acgggtcgat cccctcggag
     9361 gctgctcctg ggtgccactc tcgactcccc ccgcttgact ccgcagtctc gcgatgcgcc
     9421 gcgcagattt gccagggccg ttcgtccctg tcgaagtggg acccagccct ctacctgcga
     9481 tgaggatgct cgctgagtgg atggcacctc aggactcggg gccggacggc ccgtgacggt
     9541 cgggtcgtcc gcatcttcca cctgtcgcga cgccgggtgg agactgggat ggcctaagaa
     9601 ggatccgatg ctccagatcg acggactatc gaagcgctac ggggacgtcg tcgcgctcga
     9661 cggggcttct ttcagtgtgg catcaggtcg gatcgtcgga ttcctcggac ccaacggggc
     9721 gggcaagacg acgacgatgc gagccatctt cggcttggtg cgtcccgacg ggggcgaggt
     9781 caggtggaag gggaccgcgg tcggccccgc ggagcgagct cgattcggct acatgccgga
     9841 ggagcggggg ctctatccca agatgcgggt gggcgagcag ctcatctact tcgggcgact
     9901 ctcgggcatg agctccgcgg cggccacgga tgcggccgag acatggctcg cgcgcttggg
     9961 tctcggtgag agggtggggg cgcgactcga ggagctctcc cacggcaatc agcagcggat
    10021 ccagctcgcc gcggcgctga tccatgatcc ggagctggcc gtgctcgacg agccgttcgg
    10081 cgggttggac ccgctcggcg tggagtctct cggcgagatg ctcgcgcagg cggccgccct
    10141 cggcaccgcc gtcctcttct cgagccacca gctcgacctg gtggagcacg tctgtcagga
    10201 cgtcgtgatc atcgatcgcg ggagcgtcgt cctggcggga gcggtggacg agctcaaggc
    10261 cgcatccgcg cgccgctcgc tggagatcag ggttgacggc gagcagtggg taccggtcct
    10321 cccgagcggg accgtgacga gcaagcagga tggcgggctg cgggttctgg tcgacgcggc
    10381 ggtcgacctg gacgagatcc tggccagcgc ccggcgggcg ggggagatca cgacgttcag
    10441 cttcgagccg ccctcgctga cggatctgtt cctcgaggcc gtctcggagg ggcgaggcac
    10501 atgacagggg gatggcgttc cctgtggctg gtggcccgac gggaatggga ccagcgggtg
    10561 cgctcgctct cgttccggct ctcgacgctg ctctccatcc tcatcgtggt cgcgctcatc
    10621 gccgtgcccg agatgtatgg aggaggttcg gaacccaccc acaccgtcgg cctcgtcggc
    10681 gacagcggag cggagctgcc cgcgagcctt cgctccgcgg gcgaggagat ggggctctcg
    10741 gtgagagtcc gcgagttcgc cgacgagtcc tcgggccggg ccgcgctgcg atccgaggac
    10801 atctcggctc tgctggtcga tcagcgggac ctggtctgga gggcggaggt cgatcagggg
    10861 ctccaggcgg cggtgatctc ggcggtgcaa gccctcgatc gccgggaggc catcgcggag
    10921 ctcggcctca cctcagagga ggccgggcgg ctgatcggtc ccccggacct tcgttccacg
    10981 agcctcgagc ctgcggcggc cgagcgctcg gctcgtcgcg acctcggctt cgtcgccgtc
    11041 gtgatgctgt tcatggccat cgccttctac ggtggcttcg tcctggtcgg ggtggtggaa
    11101 gagaagtcca gccgggtggt cgaggtgctg ctcgcgcgga tccggcccgc tcagcttctc
    11161 ggggggaaga tcctcggcat cgggctcgtc ggtctggcac agttcgcgct cgtcgtgggc
    11221 tcagcgctgg tcgccgtctc gctctctggg aacacgctgc tgcccagcac caccccgagc
    11281 acgctcgggt ggatcctgtt ctggttcgtg acgggctacg ccttctactc ggtgctgttc
    11341 gcaacggccg ggtcgctcat ctcgcgacag gaggaggctc agagcgtcca gttcccggtc
    11401 acggcgttcc tcctggtggg atacgttgtg gcgttccagg ccgccgcggc gccggaaggc
    11461 gtggcggcgc tcgtcgggtc gctcctccct ccgacggctc cgatggtcat gaccgtcctg
    11521 atggcgcagg gaggggtcgc ctggtggcat gtcgcgctgt cggcggccct cctcatggcc
    11581 acgacctacg gcatggtgct gctggccgga cgcctctact cgggcgcggt gctcaggctc
    11641 ggccggcgag tgcgcttgcg ggaggcctgg cgcggcgcgg agggctgacc cggatcatga
    11701 ccccggcacc acgggcctcc cctcaccgat cgaaagacac tcaggttcgg accgtgaacg
    11761 cgctggtccc ggccccgatc tcgatgtcgt accgggcctc ggccccctcg tagtcggggc
    11821 tctgccagtc cgtcttccct gcggacgcga agcggaggtc gtcgatggtc aggttcgacg
    11881 acccgccccc cgtgcggacc ctgatgggaa ccccctccgg ccggatcacc gtgatgtcgc
    11941 tggcgccacc gccgatccgg atcgggacgg cgaccttcgg g
//
LOCUS       DASUTZ010000111         2524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1455589, whole genome shotgun sequence.
ACCESSION   DASUTZ010000111 DASUTZ010000000
VERSION     DASUTZ010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455589"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..76)
                     /locus_tag="VF097_05095"
     tRNA            complement(4..76)
                     /locus_tag="VF097_05095"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Ala,seq:ggc)
     gene            146..472
                     /locus_tag="VF097_05100"
     CDS             146..472
                     /locus_tag="VF097_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262207.1"
                     /translation="MTDDTVLARFPSEGGDLVIRPDVLELGGGQRILLEDVTAVRTRG
                     RELEIRQADGGGAAIRLSSDKEAADAEQVLNKLLSEASGDRNVTEGLEGSMGGSLGEG
                     SPPETP"
     gene            complement(476..844)
                     /gene="rsfS"
                     /locus_tag="VF097_05105"
     CDS             complement(476..844)
                     /gene="rsfS"
                     /locus_tag="VF097_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX6262208.1"
                     /translation="MTGSKQLAITAARAASDKQAADILVLEVRDLIVITDYFVIASGR
                     SDRQVKTIAESIEEALRGEGEKPLRREGRRDGRWVLLDYLDFVVHVFLDEERDFYGLE
                     RLWADAPVIDWNHAAARSGG"
     gene            complement(841..2433)
                     /locus_tag="VF097_05110"
     CDS             complement(841..2433)
                     /locus_tag="VF097_05110"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX6262209.1"
                     /translation="MKSLEELIEEIKREARGEPPEEARLEAPVFEAPGPASGPAIEPE
                     VFEAYEEWEPWLLAEVELQDEPRPAPQPEPEPVEPEPPLEPEPPIVEPRPPLPDEPEE
                     DDEDAGAAGAAPTETDPDELDFDHLLNLVGGPPAPAREDLRPARPPLEPEFLETEPLE
                     TEPMRTLEAPSPEPLPEPVPEARLRPRAAKGLRYGVLGLGILAFVIGATIWLAGPAPD
                     RDARPSGVPVGTAGDQRVVAWSVWDGQTSEGAFVTIVTLGGGKPPVALSIPSYTMVNI
                     PGYGTAAVGDAIAAEGGDRGAVAVENLLGIGIDASAFSTLDDLRRTIDELGGITIGET
                     TMSGEESVQYLMRGGADSPISAEFQFLRWQELVAGMLDAVNGRTEVFTSLGPEVGPVL
                     AAAGSAGAEVLELPVVDIGAGLARPDAEEVQRIVEQRFLPTARTTRTVRLSVLNGVGT
                     PGVGERVSKILGPAGFKLVASGNAQTFDFRETQIIATTEEFLPESELARDLLGVGKVY
                     VSAQPSGLVDVTVIVGSDFGGT"
     gene            complement(2430..>2524)
                     /locus_tag="VF097_05115"
     CDS             complement(2430..>2524)
                     /locus_tag="VF097_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinic acid mononucleotide
                     adenylyltransferase"
                     /protein_id="HEX6262210.1"
                     /translation="IRERVAKGRPIRYLVPEGVKSYIEKAGLYR"
BASE COUNT          354 a          919 c          880 g          371 t
ORIGIN      
        1 ttgtggacct gaggggagtc gaacccctga cctcctgcct gccgagcagg cgctctacca
       61 actgagctac aggcccaggc aacgaccgat tctatcagca ggcctccgcc gcttggccgt
      121 ggacggcaac gggtaagggc agggcatgac cgacgacacc gtgctggccc ggttcccatc
      181 cgagggcggc gacctcgtga tccgtcccga cgtcctcgag ctgggtggcg gccagcgaat
      241 cctcctcgag gacgtcacgg ctgtgcgcac ccgaggccgg gagctggaga tccgtcaggc
      301 cgacggagga ggggcagcga tccgcctctc cagcgacaaa gaggcggccg acgccgagca
      361 ggtcctcaac aagctcctct cggaagcgag cggtgacagg aacgtcaccg aggggctcga
      421 gggctccatg gggggctcgc tcggcgaggg ctcccctccg gagacgccct gagcctcagc
      481 cgcccgacct cgcggcggcg tggttccagt cgatgacggg ggcatccgcc cacagccgct
      541 ccagcccgta gaagtcccgc tcctcgtcga ggaacacgtg caccacgaag tcgaggtagt
      601 ccaggaggac ccagcggccg tcgcggcgcc cctcgcgccg cagcggcttc tccccctctc
      661 cccgcagggc ctcctcgatc gactcggcga tcgtcttgac ctgccggtcg ctgcggccgc
      721 tcgcgatcac gaagtagtcg gtgatgacga tgagatcccg gacctcgagg acgaggatgt
      781 cggcggcctg cttgtcggag gcggcgcgag ctgctgtgat ggccagctgc ttcgatcccg
      841 tcacgttcct ccgaagtccg atcccacgat cacggtcacg tcgaccagcc cgctgggctg
      901 agcgctgacg tacaccttac ccacgccgag gagatcacgc gcaagctccg attcggggag
      961 gaactcctcg gtcgtggcga tgatctgcgt ctccctaaag tcgaaggtct gagcgttgcc
     1021 ggaggccacg agcttgaagc ccgcgggccc gaggatcttc gagacgcgct ctcccactcc
     1081 cggcgtcccg accccgttca ggaccgagag ccggacggtg cgggtggtcc gggcggtggg
     1141 gaggaagcgc tgttcgacga tccgctggac ctcctcggcg tccgggcggg ccaggcccgc
     1201 gccgatgtcc accaccggca gctcgagcac ttcggccccc gcgctgcccg ccgcggccag
     1261 cacgggaccg acctccggtc cgagcgaggt gaacacctcg gtccgtccgt tcaccgcgtc
     1321 gagcatgccc gccacgagct cctgccaccg gaggaactgg aactcggccg agatcgggct
     1381 gtcggcgccc cctcgcatca ggtactgcac cgattcctcg ccgctcatcg tcgtctcgcc
     1441 gatcgtgatg cccccgagct catcgatcgt cctgcggagg tcgtccagcg tcgagaaggc
     1501 cgaggcgtcg atgccgatgc cgaggaggtt ctccacggcc acggcgcccc ggtcgccccc
     1561 ctccgcggcg atcgcgtctc cgacggccgc cgtgccgtag ccggggatgt tgaccatcgt
     1621 gtaggagggg atgctgaggg cgacgggcgg cttcccaccg cccagggtga cgatcgtgac
     1681 gaaggcaccc tcgctcgtct gcccgtccca gaccgaccag gccacgacgc gctggtcgcc
     1741 cgcggtgccg acgggcacgc ccgatgggcg cgcatcgcgg tcgggcgcgg gacccgcgag
     1801 ccagatggtg gcgccgatca cgaaggccag gatcccgagg cccagcaccc cgtatcggag
     1861 gcccttggcc gcccggggcc tgagccgggc ctcggggacc ggctcgggga ggggctccgg
     1921 ggaaggtgcc tcgagcgtcc tcatgggctc ggtctcgagg ggctcggtct cgaggaactc
     1981 gggttcgagg ggcggcctcg cggggcggag gtcctcccgc gccggggcgg gcgggccacc
     2041 caccaggttc aggagatggt cgaagtccag ctcgtcgggg tccgtctcgg tgggggccgc
     2101 gcccgccgct cccgcgtcct cgtcgtcctc ctcgggctcg tcggggagcg gcgggcgcgg
     2161 ctccacgatc ggaggttcgg gctccagcgg cggctccggc tcgacgggct ccggctccgg
     2221 ctgaggcgcg ggccggggct cgtcctggag ctcgacctcg gccagaagcc agggctccca
     2281 ctcctcgtac gcctcgaaga cctcgggctc gatcgcggga cccgaagcgg gcccgggggc
     2341 ctcgaacacc ggcgcctcga gccgcgcctc ctccggaggc tcaccgcgcg cctcgcgctt
     2401 gatctcctcg atcagctctt ccaggctctt catcggtaca ggcccgcctt ctcgatgtag
     2461 ctcttcacgc cctccggcac gaggtaccgg atgggcctgc ccttggcgac gcgctcccgg
     2521 atgt
//
LOCUS       DASUTZ010000112         7463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_29968, whole genome shotgun sequence.
ACCESSION   DASUTZ010000112 DASUTZ010000000
VERSION     DASUTZ010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7463
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29968"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1563
                     /locus_tag="VF097_05120"
     CDS             <1..1563
                     /locus_tag="VF097_05120"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX6262211.1"
                     /translation="RLWVAMFIALALFLIFLLFLPIGASMERAENAFLRRLVTLRSDP
                     LDSVMRFVNGLGAEWTLRIIRWSVLIVLIAVRRWRHLLIFLGTLLLVAWLVNKLSLIA
                     PRARPLGVEIIGPWEGFAAPSSPVAALSVALAGVTYTLAVPGRPRRLVKWATWIAIGA
                     LGLSRIYLGVDHPSDVLFALIIGVGIPLVAYRFFTPNTVFPVTYTKGRAAHLDVGGRR
                     GEAIRQAIAEQLGIAVVELKRFGLHGSGGSTPLRIEVEGEKDRYLFGKLYAKSHLRAD
                     RWYKLGRTILYGTLEDEKPYNSVRRLVQYEDYVLRVMHDAGLPCARSYGFVEITPERE
                     YLLVTEFLEGGVEISEAEVDDDLIDDALAAVRTMWDAGLAHRDVKPANIMVREKRVFL
                     IDPAFGEIRPSPWRQAVDLANMMLVLALSADADTVYERALRIFSEEEITEAFAATKGV
                     TMPTQVRNLLRERRKQGEDILGRFRKLAKARRPISIQRWSLQRVGVLLAAILGSLIAL
                     GLLLDNLRTGAL"
     gene            1560..2108
                     /locus_tag="VF097_05125"
     CDS             1560..2108
                     /locus_tag="VF097_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262212.1"
                     /translation="MRRVLAMGALVALVGCGTPEQSIPTCPPDATDALVLVAQSVPSA
                     TQIPCVTELPVGWSYGGQIIERGRTELWLYSDRAGEQAVTVTLTPRCAFSGAVEVPSD
                     EPALTRYEEPISLTPVLSVNRYYVFEGGCVTYRFRFHEGGSYTEVLQATEALSFVSRT
                     EGAEALREAGLILCGAGVDCPG"
     gene            complement(2110..3210)
                     /locus_tag="VF097_05130"
     CDS             complement(2110..3210)
                     /locus_tag="VF097_05130"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HEX6262213.1"
                     /translation="MDKRSRKRAFRIAEIVVSIAIVVGIFAFAIPKFASYGDVWAAVQ
                     EMTWLEIATLVGISVFNIATYWPMMVASMPGLTFGQAAVNNQSSSSIANTLPGGGLLA
                     TGVTYTMYRSWGFTNTQIGLTALITGIWNAFVKLAMPVMALAALAITGQGSTTLLIPA
                     LIGLAILLAAVALFAGVLWKKSLARAIGGGLGKVASAARRVVRKPSVHWGEAAVRFRK
                     QTLGLLSRRWPALTISALVSHFSLYLVLLVALRHVGVSDQEIGWAQALGVFAFVRLIT
                     LVPLTPGGVGLVELGYIGGLYVAGRDAADVPIELFRAQITAAVLVFRTLTYALQIPTG
                     ALTYVIWQRKRSWRKPVPTEEPPETAMAATPG"
     gene            complement(3232..3936)
                     /locus_tag="VF097_05135"
     CDS             complement(3232..3936)
                     /locus_tag="VF097_05135"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX6262214.1"
                     /translation="MRRTHEHPLLMNRRLDVVTAAVLLALGTILLALMAFGETREILQ
                     GIDDAAARWFVSVRWVPLTGLAHLLNLLGSVWVTLPVRIAAGAYLAITRRWWHLLAFA
                     SAMLLSEASVSILKALYDRPRPVGSLVETSGASFPSGHAVAASVTTVALVIALFPPAG
                     LHRWLWGAAAAVFAFAMALSRAYLAAHWLSDAVTGTFLGVSIALVTALIVEWIRGPDV
                     AEEPARETVPRGPGTG"
     gene            3964..4917
                     /locus_tag="VF097_05140"
     CDS             3964..4917
                     /locus_tag="VF097_05140"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6262215.1"
                     /translation="MTDVLGTRSKTVRPGACRAVLLLILILAAACVGRPSETTSIPGV
                     DLITIGSFDFSESEILAEIYAQALTFRGYRVERVLRVGPRELVEPALEEGLIDLVPEY
                     LGTALEFMTHGRGTATADVDASHQALQRELSARGIAVLEAAPAQNANGVVVTRETAAR
                     YGLERISDLAPHASELVIGGPPECPERPLCLQGLGRLYGLRFREFVPTDAGGPLTAGF
                     LDAGEIDVAVLFTTSGYLGEDAYVLLEDDRGLQPAENVVPVVRGEVLRRYGAPLRDLL
                     DDISSRLEAEDLIALNRSVELEGRDPARAALGWLTAHGYAG"
     gene            5034..5285
                     /locus_tag="VF097_05145"
     CDS             5034..5285
                     /locus_tag="VF097_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262216.1"
                     /translation="MAVDERARHQLYNRVVEVLGEEEAVILMAHLPPGGYPNFATKQD
                     LLDLERRMTAELKSHTFRVVFAANLTTVGVLTAVVAALA"
     gene            5352..6962
                     /locus_tag="VF097_05150"
     CDS             5352..6962
                     /locus_tag="VF097_05150"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6262217.1"
                     /translation="MSRAGPHDAIVVGGGHNGLVAAAYLARRGLRTLVLERREIVGGA
                     CVTEEIAPGVRASTTSYIASMLRPEIIRGLDLPRHGLRMVPCEPGLQVAFEGGEPLAW
                     WNDVGRTAAGIERRSPKDARAFVDLDAELKRLARYLQPYFLEPPPDLDARGVRRLPDL
                     LRAWRRLRKVNSRDAAGLVSLLTGSLADLARRRFESEEVRALLLANNLYGKHGGPFEA
                     GSAVGLIFHLLSGGPEDAQGYMGHVIGGMGAITQALASAAREAGAEIRSEAEVERVLV
                     RNGAATGVAMTDGTELEATVVLSNADPRRTFLGLVEKQELPDDFRADVEAIRMDGPCA
                     KVNLALSEEPRVVGLPADADPGRRALFTLVPSLDFAQRSADDARRGLLSDSLWVDCVV
                     ASNVDPTLAPEGTHVMTCFVQYVPYRLAEGTWDERREELGDRVIGTIARSVPNVPGSV
                     LARQVLTPLDLERTYGLTEGNIFHGDLSLDRLFFMRPVPGWSGYRTPIHGLYLCGAGA
                     HPGGGVTGAPGYNAARRVLGDLRRRGIR"
     gene            6959..>7463
                     /locus_tag="VF097_05155"
     CDS             6959..>7463
                     /locus_tag="VF097_05155"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX6262218.1"
                     /translation="MKGLLERLEDGVVLGDGGYLLELEKRGYVRAGPFTPEVSITNPD
                     ALAQLHREFLRSGAEILQALTFYASEEKLATVGLAGRVDDINRAAVEVARSVAKEGDA
                     LVAGNLSLTWAYDPADPASPDRVRALFDRQLEVQMDVGIDLVVAETFTWLGEALIATE
                     RALTTGLP"
BASE COUNT         1097 a         2594 c         2693 g         1079 t
ORIGIN      
        1 cggctctggg tcgcgatgtt catcgcgctg gccctcttcc tgatcttcct cctcttcctc
       61 cccatcgggg catcgatgga gcgcgcggag aacgccttcc tgcgccggct cgtcacgctg
      121 agatccgacc cgctcgactc cgtgatgcgg ttcgtgaacg ggctcggggc cgagtggacg
      181 ctgcgcatca tccgctggag cgtcctcatc gtcctgatcg ccgtccggcg gtggcgtcac
      241 ctgttgatct tcctggggac cctccttctc gtggcctggc tcgtgaacaa gctgtccctg
      301 atcgccccgc gggcccggcc cctgggggtg gagatcatcg ggccgtggga gggcttcgcc
      361 gccccgtcga gcccggtggc ggcgctgtcc gtggccctgg ccggcgtgac ctacaccctc
      421 gccgtgcccg gccgtccccg ccggctcgtc aagtgggcga cgtggatcgc catcggggcg
      481 ctcgggctct ctcgcatcta cctgggggtg gatcacccgt cggacgtcct cttcgccctg
      541 atcatcggcg tcggcatccc gctcgtggcc taccggttct tcacgccgaa cacggtgttc
      601 cccgtgacgt acacgaaggg ccgcgccgcg cacctcgacg tcggggggcg acggggcgag
      661 gcgatccggc aggcgatcgc cgagcagctg ggcatcgccg tggtcgagct gaagcgcttc
      721 ggcctgcacg gctccggggg ctcgaccccg ctgaggatcg aggtcgaggg ggagaaggat
      781 cgctacctct tcgggaagct ctacgccaag tcgcatctcc gcgccgaccg ctggtacaag
      841 ctcgggcgga ccatcctcta cggcacgctg gaggacgaga agccgtacaa ctcggtgcgc
      901 cgcctcgtgc agtacgagga ctacgtgctg cgcgtgatgc atgacgccgg cctcccctgc
      961 gcccgctcgt acgggttcgt ggagatcacg ccggagcgcg agtacctcct cgtcaccgag
     1021 ttcctcgagg gcggggtcga gatcagcgag gcggaggtgg acgacgacct catcgacgac
     1081 gcgctggccg ccgtgcgcac gatgtgggac gcgggcctgg cacaccggga cgtgaagccc
     1141 gccaacatca tggtccgtga aaagcgggtc ttcctgatcg acccggcctt cggcgagatc
     1201 cggccctcgc cctggaggca ggccgtggat ctcgccaaca tgatgctcgt cctcgccctc
     1261 agcgccgacg ccgacaccgt gtacgaacgg gccctgcgga tcttctccga ggaggagatc
     1321 accgaggcct tcgcggccac caagggcgtg acgatgccca cccaggtccg gaacctgctg
     1381 cgcgagcggc gcaagcaggg ggaggacatc ctgggccggt tccggaagct ggccaaggca
     1441 aggcggccca tctcgatcca gcggtggagc ctccagcggg tgggggtcct gctcgcggcc
     1501 atcctcgggt cgctgatcgc gctgggcctc ctcctcgaca acctgcggac gggggcgctg
     1561 tgaggcgcgt tctcgcgatg ggcgcgctgg ttgccctggt gggctgcggg acgccggagc
     1621 agtcgatccc gacctgtccg cccgacgcga cggacgcgct cgtcctggtc gcgcagtcgg
     1681 tgccctccgc cacccagatc ccgtgcgtca ctgagctgcc ggtggggtgg agctacgggg
     1741 ggcagatcat cgagcggggg cgaaccgagc tctggctcta ctcggatcgc gcgggcgagc
     1801 aggcggtcac cgtcacgctc accccacggt gcgccttctc cggcgcggtg gaggtcccca
     1861 gcgacgagcc cgcgctgacc cgctacgagg agccgatctc gctgaccccc gtcctcagcg
     1921 tgaaccgcta ctacgtcttc gagggcgggt gcgtgaccta tcggttccgg ttccacgagg
     1981 gcggctcgta caccgaggtg ctccaggcca cggaggcgct cagcttcgta tcccgaacgg
     2041 agggggcgga ggcgctgcgc gaggccggac tcatcctgtg cggggccggg gtggactgcc
     2101 cgggataggt cagcccgggg tcgccgccat cgccgtctcg gggggctcct ccgtagggac
     2161 gggcttccgc cagctccgct tgcgctgcca gatgacgtag gtcagcgctc ccgtcgggat
     2221 ctggagcgcg taggtgaggg tgcggaacac gagcacggcg gcggtgatct gggcccggaa
     2281 cagctcgatg ggaacgtcgg ccgcgtcccg cccggccacg tagaggcctc cgatgtaccc
     2341 cagctcgacg aggccgacgc caccgggcgt gagcggtacc agcgtgatca ggcggacgaa
     2401 ggcgaacacg ccgagcgcct gcgcccagcc gatctcctga tcggacaccc ccacgtgccg
     2461 gagcgccacc agcagcacga gatagagcga gaagtggctg accagcgcgg agatggtgag
     2521 agcgggccac cgcctcgaga gcagcccgag ggtctgcttg cggaaacgga ccgcggcctc
     2581 gccccagtga accgagggct ttcgcaccac ccttcgcgcc gccgaggcca ccttccccag
     2641 gccgccgccg atggcgcggg ccagcgactt cttccacagc accccggcga acagcgcgac
     2701 ggccgccagc aggatggcga gaccgatcag ggccgggatg aggagggtgg tgctcccctg
     2761 ccccgtgatg gccagggccg ccagggccat caccggcatg gccagcttca cgaacgcgtt
     2821 ccagatcccg gtgatgagcg cggtgaggcc gatctgcgtg ttggtgaaac cccacgagcg
     2881 gtacatcgtg taggtgaccc ccgtcgcgag gagcccccct cccggcaggg tgttcgcgat
     2941 cgagctcgag gactggttgt tgacggcggc ctggccgaag gtcaggccgg gcatcgaggc
     3001 caccatcatc ggccagtacg tggcgatgtt gaacaccgag atgccgacga gcgtggcgat
     3061 ctccagccag gtcatctcct ggaccgcggc ccacacatca ccgtagctgg cgaacttggg
     3121 gatggcgaag gcgaagatcc ccaccacgat ggcgatggag acgacgatct ccgcgatccg
     3181 gaacgcacgc ttccgggatc tcttgtccac gcgtcagctc ccggacgaag cctagcccgt
     3241 cccggggcct cgaggaacgg tctcgcgagc gggctcctcc gcgacgtcgg gaccccggat
     3301 ccactccacg atcagggcgg tcacgagggc gatgctcacg ccgaggaagg tcccggtgac
     3361 cgcgtcggag agccagtgcg cggcgaggta ggcgcgggac agagccatgg cgaaggcgaa
     3421 caccgctgca gcggcccccc acagccaccg atggagtccg gcgggaggga agagcgcgat
     3481 gaccagggcc accgtcgtca ccgaggccgc caccgcatgg cccgaaggga acgaggcccc
     3541 gctcgtctcg accagggagc cgaccggccg ggggcgatcg tagagggcct tcaggatcga
     3601 gacgctcgcc tccgagagga gcatggccga ggcgaacgcg agcaggtgcc accaccggcg
     3661 cgtgatcgcc aggtaggcgc ccgccgcgat ccggacgggg agggtgaccc acacggaccc
     3721 cagcaggttc aggaggtgag ccaggcccgt cagcgggacc caccgcacgg agacgaacca
     3781 ccgggcggcg gcatcgtcga tgccctggag gatctccctc gtctccccga aggccatcag
     3841 ggccagcagg atcgtcccca gggcgaggag aactgcggcc gtcacgacgt ccagccgccg
     3901 gttcatcagg agcggatgct cgtgggttct gcgcattcag caacaagagt agtgttccgg
     3961 atcgtgacgg acgtcctcgg gacgcgatcg aagacggtcc ggccgggggc gtgccgggcc
     4021 gtgctcctcc tgatcctgat cctggccgcg gcgtgcgtgg gccggccctc cgagacgacc
     4081 agcatccccg gggtggacct gatcacgatc ggctccttcg acttctctga gagcgagatc
     4141 ctggccgaga tctacgccca ggcgctcacg ttccgcggct atcgggtcga gcgggtcctc
     4201 cgggtcggtc cgcgagagct ggtggagccg gcgctggagg aggggctgat cgacctcgtc
     4261 cccgagtatc taggcacggc gctggagttc atgacgcacg ggcgcgggac ggcgaccgcg
     4321 gacgtggacg cctcccacca agcgctccaa cgggagctct cggcgcgggg gatcgcggtg
     4381 ctggaggcgg cccccgccca gaacgcgaac ggggtcgttg tgacccggga gacggcggca
     4441 aggtacgggc tcgagaggat cagcgacctc gccccccacg cgagcgagct ggtgatcggc
     4501 gggccccccg agtgcccgga gcgtcccctg tgcctccaag gcctcgggcg tctgtacggc
     4561 ctccggttcc gggagttcgt ccccaccgac gccggcggtc ccctgacggc cgggttcctg
     4621 gacgccggag agatcgacgt cgccgtgctg ttcaccacga gcggatacct gggcgaggac
     4681 gcatacgtcc tcctggagga cgaccggggc ctccagcccg ccgagaacgt cgtcccggtc
     4741 gtgcgggggg aggtcctccg ccgctacggg gcgccgttgc gggacctcct cgacgacatc
     4801 tccagccggc tcgaggccga ggatctcatc gcgctgaacc gttcggtcga gctggagggc
     4861 cgggaccctg cccgcgccgc gttgggctgg ctcaccgccc acggctacgc gggctgagcc
     4921 ctccacgatt tgcccattcc aacttctcct gaggggccgg cgcggccccg ctccatgtcg
     4981 gacgcggccg atacgatcgg agcactcccc ctcacgacac caagggggag ataatggccg
     5041 tcgatgagcg cgccaggcac cagctgtaca accgagtcgt ggaggttctg ggcgaggagg
     5101 aggccgtcat cttgatggcc catttgccgc ctgggggtta cccgaacttc gctacgaaac
     5161 aggacctcct cgatctcgag cggaggatga ccgctgagct caagtcccat acctttcggg
     5221 tcgtcttcgc ggccaacctg acgacggtcg gcgtgctcac cgcggtggtc gccgcgctcg
     5281 cctgagcggc cgtctcgcct ccccggtata gtccctgggc cgacctgagc aggccggcgt
     5341 ctgggggagg catgtcgcga gccggtcccc acgacgcgat cgtcgtgggc ggtgggcaca
     5401 acggcctggt cgccgcggcc tacctggcgc gacggggcct gcggaccctc gtcctggagc
     5461 ggcgcgagat cgtgggcgga gcctgcgtca ccgaggagat cgccccgggc gtccgtgcct
     5521 ccaccacctc gtatatcgcc agcatgctgc ggcccgagat catccgaggt ctcgacctcc
     5581 cgaggcacgg cctgcggatg gtgccgtgtg agccgggcct gcaggtcgcg ttcgaaggcg
     5641 gcgagccgct ggcctggtgg aacgacgtgg ggaggacggc agccgggatc gaacggcgct
     5701 cgcccaagga tgcgcgggcg ttcgtggacc tcgacgccga gctgaagcgg ctggcgaggt
     5761 acctccagcc ctacttccta gagccgccgc ccgacctcga cgcgcgtggc gtgcgccgtc
     5821 tgcccgacct cctccgcgcg tggcggcgtc tgcgcaaggt caactcgcgc gacgcggccg
     5881 ggctcgtctc cctgctgacg gggtcgctgg cggacctcgc gcgtcggcgg ttcgagtccg
     5941 aggaggtccg cgccctgctg ctcgccaaca acctctatgg gaagcacggc gggcctttcg
     6001 aggcggggtc ggcggtgggc ttgatcttcc acctcctctc gggcgggccc gaggacgcgc
     6061 aggggtacat ggggcacgtc atcggaggga tgggggcgat cacgcaggcg ctggcctcgg
     6121 cggcgcggga ggcgggagcc gagatccggt ccgaggccga ggtggagcgc gtgctcgtcc
     6181 ggaacggggc cgcgaccggc gtggccatga ccgatggcac ggagctcgag gcgaccgtcg
     6241 tgctgtcgaa cgcggacccc aggcgcacgt tcctggggct cgtggagaag caggagctgc
     6301 ccgacgactt ccgggccgac gtcgaggcga tccggatgga cggcccctgc gccaaggtca
     6361 acctcgcgct gagcgaggag ccgcgcgtcg tcggcctccc ggcggacgcg gaccccggcc
     6421 gccgcgccct gttcaccctg gtgccgtcgc tggacttcgc gcaacggtcg gccgacgacg
     6481 cgcggcgcgg cctcttgagc gactccctct gggtggactg cgtcgtggcc tcgaacgtgg
     6541 accccaccct cgcccccgag ggcacccacg tcatgacgtg cttcgtccag tacgtgccct
     6601 accggctggc cgaagggaca tgggacgagc ggcgggagga gctcggcgac cgggtgatcg
     6661 ggacgatcgc gcggtccgtg cccaacgtgc ccggctcggt gctcgcgcgc caggtcctga
     6721 ccccgctcga cctggagcgc acctacggcc tcaccgaggg gaacatcttc cacggcgacc
     6781 tgagcctcga ccgtctcttc ttcatgcggc cggtccccgg ctggagcggc taccgcaccc
     6841 cgatccacgg cctgtacctc tgcggcgcgg gggcgcaccc gggtggcggc gtgaccgggg
     6901 cgcccggcta caacgcggcg cgccgggtcc tgggcgacct gcgccgaagg gggatccgat
     6961 gaagggcctg ctggagcggc tcgaggacgg cgtggtcctc ggcgacggcg gctacctgct
     7021 cgagctggag aagcgaggct acgtgcgtgc ggggcccttc acgccggagg tgagcatcac
     7081 gaaccccgac gcgctggccc agctccaccg cgaattcctg cggagcgggg ccgagatcct
     7141 ccaggcgctc accttctacg ccagcgaaga gaagctggcc accgtcgggc tggccgggcg
     7201 cgtggacgac atcaaccgag cggcggtgga ggtggcgcgc tcggtggcga aggagggcga
     7261 cgcgctcgtg gcgggcaacc tcagcctgac gtgggcgtac gacccggccg acccggcgtc
     7321 acccgaccgc gtccgcgcgc tgttcgaccg ccagctcgag gtgcagatgg atgtcggcat
     7381 cgacctcgtc gtggccgaga cgttcacctg gctgggcgag gccctgatcg ccacggagag
     7441 ggcgctgacg acaggcctgc ccg
//
LOCUS       DASUTZ010000113        13546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_678352, whole genome shotgun sequence.
ACCESSION   DASUTZ010000113 DASUTZ010000000
VERSION     DASUTZ010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13546
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678352"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VF097_05160"
     CDS             <1..522
                     /locus_tag="VF097_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262219.1"
                     /translation="GLVLLLALLVAALARQIGVLHMRLGPRGALEVDEEGPPLGEMPP
                     PAEVVDLEGAARRVGGPGAPTLLLFVSPGCPICREVLPGVAPAARAGDLHPLVLVDDD
                     RPEALRVYRERDPRVPVAGAPGVAELYDVPGTPFAVVLDAAGAVRAKGTVNTLEQMEG
                     LVDTARRRLEAAS"
     gene            522..1046
                     /locus_tag="VF097_05165"
     CDS             522..1046
                     /locus_tag="VF097_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262220.1"
                     /translation="MGAVERLGRGLARRTSRRSFLGRIGGTLVAMAGGSFVVAALDPE
                     RAEAYHICGHIFTTGSCPHPYFPNTRIDRYGFPVHPVHGYPVDDDGRIYTSRSQRRRR
                     ICEGIVAERYPFTKPVVLEGTWDRCCGGRIRRVVDCCSRSRTRINGDASLVGYCHGGR
                     RVFCITYIETEIRC"
     gene            1127..2302
                     /locus_tag="VF097_05170"
     CDS             1127..2302
                     /locus_tag="VF097_05170"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEX6262221.1"
                     /translation="MAGTITPVVHGGRRTGWGVSVALHALGAGLAAAAFGAVLGGGGA
                     LLGGPWGGAGLWAVALVAGAYALREAFGLPVPVPERRRQVPEWWRGRLGPRGAAFAYG
                     LGLGVGFLTYLRHGTLVAVSAVALASGDPWIGALVLAPFGLARGLSLTAVASTRTSDE
                     VRGVIDRLERIAVSALPRGANAAALVVLSAVALGAAAFAPLDAASVGALAAGIVAAVF
                     AWAVAGKLLHWTVWRGTLPGYRLGPMEPVAAVGAPALEAVVVVLVLAGLAAAAGAVAL
                     ALLAAASAALLRARGGGEAPCACFGRRRVRSVRSLLIRNGAIAAAAAASLWAESRPPS
                     LPPADPLPLALALIGAAAAIALGVSAARWLRPPAAAGGTSPRARATSPGTPAGTSGR"
     gene            complement(2217..3602)
                     /locus_tag="VF097_05175"
     CDS             complement(2217..3602)
                     /locus_tag="VF097_05175"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262222.1"
                     /translation="MSRNRAAALAIALSAAALAGLPLASEAHAARADLGLAVRAPGPS
                     PVGSPVSLAIEVTNAGPDAEPEAMLEVTLPAGLVLASALPEVGSCVPGPPVACALGSV
                     GPGETVTVAVEASPVKPGALEGSAAVSGVVAGAHEAALVVEATGQPCTIEGTEGDDEL
                     QGTQGPDVICGLGGDDVLRGRGGDDELLGGAGRDTADFSASLGGVRVDLAAGTAHGEG
                     ADVLAEIEDATGSAHDDLLVGSAEGSTLSGGGGNDVVLGEGGDDALLGGEGGDHLDGG
                     AGSDAIDGGPGADTCLLGADGGTRTACEAAPNPFDPRDVRGPLDLKRVRGPKGTSQLT
                     WNLRTRPRWTKKLAWDKAYLLVWIDARGGPQPDHVAVIRSTGRRMIGRLFRVSPAGRE
                     RQIGKVRAWKRGKRGAAVRVALHKVTVGPDRFSYRWSAQTMFTGPRCRRVCLDSSPGP
                     GEMFLRPLPGA"
     gene            3661..4833
                     /locus_tag="VF097_05180"
     CDS             3661..4833
                     /locus_tag="VF097_05180"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HEX6262223.1"
                     /translation="MNLLDAAILVLLALSVVNGFRRGAMLQVITFGGLLVGLVVGAVL
                     APQLASLADDPAWQSLIALGSLLLIAGLFDAAGWAIGTRAWTAARRSHLGVVDSWAGV
                     GVAAVSTLLVVWFLAFNLVQGPIPALSREIRASSIVRGIDAMLPRPPSILAQVRSFLD
                     RFGFPEVFQGLPPAPAGPVEGPSRGEVGRAVDAAAPSTLRVVGRACDRIQAGTAFIVA
                     DGYAVTNAHVVAGVDAPQVQQQSGGSIDATTVLFDPDLDLAVLRIATTPAAPLDLLAG
                     NLDRGQGAAVLGYPQGGGLTVGPAAVRRVMQAVGRDIYGRDTVSREVYELQAEVRPGN
                     SGGPFVTADGDVAGIVFAASVGNDDVGYALTSTEALPRVERGIGRTEPTDTGPCLR"
     gene            complement(4838..7051)
                     /locus_tag="VF097_05185"
     CDS             complement(4838..7051)
                     /locus_tag="VF097_05185"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEX6262224.1"
                     /translation="MTSGPEPPDSPHFEDALERILNEGAPEIVTDLPSKVLATKEGAT
                     FLYADSEGHLDDRHELGLGFYHLDTRFLSYFRFKLSGRDPILLSSSAERAYMAHIDMT
                     NPALYDGDGRVAAAQQSLNVRRIRAVGDRLFEKTRIKNYNARAVEVDVTYTFGADFAD
                     IFEVRGLRRQRRGHHEPPKAEGRSISFTYVGEDDVQRTTRIAFGAEPDRMEVRGDVVE
                     ATFGLSIPSGETRVLSLQVQPIVGPGRELPARGYDPTIHELRRSYESWERESTRIRTD
                     NELFNELLRRGLRDLRALWMSADGGATLAAGIPWYVSPFGRDALITSHQLLMVNPQLA
                     RETLALLASLQARERDDWRDAEPGKILHEMRRGELAGAGMIPHTPYYGSVDSTPWFLI
                     LLAQYHRWTADTELVRELMPAAEAALAWIDDHGDLDGDGFVEYLRRSDRGLENQGWKD
                     SWNSVVHRDGTLAKPPIALVEVQAYVYMAKVRMAGLFASLGDHARAVALRDETTVLRK
                     RFNDEFWMEDEGYFAEALDAKKRQVRAITSNPGHALYCDAVEPAKAEAIAQRLFAPDM
                     FSGWGIRTLSKSERAYNPMSYHNGSVWPHDNALIAAGLKRYGFVEATNRVATAMFEAA
                     ITMDYMRLPELFCGFVRRSPNRPVQYPVACSPQAWAAGAPFLLLQAMLGVSARADENL
                     LTINKPHLPRWLNEVELRNLRVGESTLSLTFRREGEVTAFTLASKEGDVRVVMEE"
     gene            complement(7145..12352)
                     /locus_tag="VF097_05190"
     CDS             complement(7145..12352)
                     /locus_tag="VF097_05190"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX6262225.1"
                     /translation="MRYQVLGPIRASTDRGFVELGGPKQRLVLALLLADANHVVAEDR
                     LIDGVWGEDAPEGARHTLQTYISELRSLLDDQIAREGKGYRLAVDPDAVDAHRFDSLV
                     EAARERLGDAPAGAAELLREALALWNGEPYADLGDAPGMLPEVSRLQQSRLSAVEDRV
                     RADLECGRHVELVAELEALTREHPYRERLRELHMLALYRCGRQADALRAFERTRDLLA
                     DELGIDASPELRSLHRQILVQDPELVPATGPEDRPAHGATEEAGPAGFHVVRGFELRE
                     RVGSGDFGVTYRAYQASVGREVAIKVIRPEYSNAPSFVRGFEPRTRRIAEPSHANALP
                     LLDSWRDPGGAYLVTPWVPGGSVRRALRHGPWSLAATLRMLEQVGAAIASAHRHGVVH
                     GNIKTSNVLLDDEGNALLTDFAIVPRAVDAAGAPQTAAPASVSPEERRGQPLSPASDL
                     YALAVLAVEALTGATGPTENPASGLAAARPDLPPGLQSVLARATDPDPHRRQARVEDV
                     LRELRRAAGTDAVAMVELVEDSEPVRNPYKGLQPFGEADALDFFGREALTDRLVEAVA
                     TRRLVAVVGPSGSGKSSAVRAGLLPALRAGAVPGSRAWLVTEMFPGSYPFEELEVALL
                     RIAIDPPDGLLAELMADDRGMLRAIKQILPGDDSELVLVVDQFEELFSLTARSDLRQM
                     FLDGLVALAEDARGRVRIVLTLRADYFDRPLEHHAFGGLVRAGLVPVTVPSPEELARA
                     VSGPAERVGVEYEPGLVAAIVRDVVDQPGGLPLLQHALTELFARRDGRTLTAAAYRET
                     GGVLGALARRAEEVYGSLPPAGQEVAKQVFLRLVSVDEHAQDARRRVSRRELAGLGAD
                     PKAIDRVVRQYGAHRLLSFDRDPVTRGPTVEVAHEALFREWGSLRRWIESRREDLLLH
                     RRLAAAVSEWRDAGRLDDYLLGGGRLEEYERWAATTRLTLTEEERAYLADGRAREDRR
                     SARRRRRRLGIVGGSIAAALVASTLALVALVQRDRAAEERSAAEAQATLADARRLATQ
                     ALSEERLDRSLLLALAAVRLDDSTESRRSLLAGLVRSPDAIGIMQGPGRFLESIAISP
                     DGRILAVASSTGDLAFFDAETRGRLGQPVHLEGTVDRSSGSLVFSPDGRTLVVATQGP
                     GDAPSHLDLVDVTDRTIERIELPPGVGAESVAVSHDGATLAAATHEFPKGANPFGPPP
                     RKAVLLFDARTGALQGRLSLSRRDLEGPTLNLAFLPGDARLVTSTDRGTTVLWDVAHR
                     EPVRSFDVGGPMGMSPDGSRAAIGGEDGSLIVLDLKSGEQRDLVGGHDAPITAVVFDP
                     NGTLIATTSADRAAMVWDLESELPPWAPAGGPTMTLPGHAGTVSDSVVDPDGETLYTS
                     GHDGLVIVWDLGGRRRFGRRLDRGIGAAEYSQISALSPQGDVLAVPQADGTILLWDVA
                     TQEELGRLAATGDVPVNGLAFSPDGRTIASTGASGIVVLRNVETGSTIARLRPRPKPQ
                     GGGWWVTFSPDGTRLATTHSPDPAGLRTRAIVWDARARRPLKELDLEGGAAEIDFSPD
                     GRLLSIVTCCADGVSDVGVWEVATWRRILSPDLDSLVLQTDFSPDGGTLVAGDFDGNV
                     TFWDIASRRPDGSGLTGLQEVISVDVSPDGSLVAAADSTGAVGVWRYPSRDRIGTFPA
                     TDFPANVAFTADGRRLAVVHITDAAFIWDLDTASWKRHACLVAGRSLSRDEWRALLPD
                     RPYAPLCPG"
     gene            12602..13189
                     /locus_tag="VF097_05195"
     CDS             12602..13189
                     /locus_tag="VF097_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262226.1"
                     /translation="MRELLVVPLVLAALLVAAAPVGAEQPLSFERFEFEAGPFPDPFI
                     LEVCGLEVLTSSQAKGTITLFTDGTLKEHVNLVITSTNPDNGVTTTERDAFTFFSEPF
                     EAVIDEEAGTITRVFREAFVGLPFRQMLPGGGVLIRDAGKVVLLVTIVEDLRTGEELS
                     VEVEELEVRGPHPAGPLDLEERYRPLCDALAGEGR"
     gene            13245..13544
                     /locus_tag="VF097_05200"
     CDS             13245..13544
                     /locus_tag="VF097_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262227.1"
                     /translation="MRRLKRSFARIAMVATVAGALLTGSPAGATEPVGGCAPPFEEPI
                     LIEDLALPVLVEFAQAIDTATGANRDGYVCFMPLATGNQTGWLQLVNILDNRLPR"
BASE COUNT         1810 a         4884 c         4915 g         1937 t
ORIGIN      
        1 ggcctggtcc tgctcctcgc cctgctcgtg gccgcgctgg cccgccagat cggcgtcctc
       61 cacatgcggc tggggccgcg cggcgccctc gaggtcgacg aggagggacc gccgctggga
      121 gagatgccgc cgccggccga ggtcgtcgac ctcgagggcg ccgcgcgccg cgtgggcggc
      181 cccggcgccc cgaccctcct gctcttcgtc tcgcccggct gcccgatctg ccgcgaggtc
      241 ctcccggggg tggcgcccgc cgcgagggcc ggcgacctcc acccgctcgt cctcgtggac
      301 gacgaccggc ccgaggcgct ccgcgtctac cgggagcgcg accctcgcgt ccccgtggcc
      361 ggcgcccccg gggtggccga gctctacgac gtccccggga ccccgttcgc cgtcgtgctg
      421 gacgcggcgg gcgcggtccg ggccaagggc accgtgaaca ccctcgagca gatggagggg
      481 ctggtggaca cggcccggcg ccgcctggag gccgcttcct gatgggagcg gtggagcggc
      541 tcggccgcgg cctggcccgg cggacgagcc gccggtcgtt cttggggcgc atcgggggga
      601 ccctcgtggc gatggcgggc ggctcgttcg tggtggccgc cctcgacccc gagcgggcgg
      661 aggcctacca catctgcggc cacatcttca ccacgggctc gtgcccgcac ccctacttcc
      721 ccaacacgag gatcgaccgg tacggcttcc cggtccaccc cgttcacggc tacccggtcg
      781 acgacgacgg gaggatctac acgtctcggt ctcagcggcg ccgcaggatc tgtgaaggga
      841 tcgtcgccga gcggtacccg ttcacgaagc ccgtcgtgct cgaggggacc tgggaccggt
      901 gctgcggggg gcggatccgc cgggtggtgg actgctgctc gcgctcccgg acgaggatca
      961 acggcgacgc ctcgctggtc ggctactgcc acggcgggcg ccgcgtcttc tgcatcacct
     1021 acatcgagac cgagatccgg tgctgagcct ccccctcctg atcgtggcgc tcaccgcgtc
     1081 gctgatcgcc gggctgtcgg ccctgttcgg cccctgaggg atgtcgatgg ccgggaccat
     1141 cacccccgtc gtgcacgggg gacgccgaac cggctggggg gtcagcgtgg ccctgcacgc
     1201 gctgggggcc gggctggcgg cggcggcgtt cggggcggtc ctgggcggag ggggcgcgct
     1261 cctgggcggg ccctggggcg gcgccggcct gtgggccgtg gcgctcgtgg ccggggccta
     1321 cgccctgcgc gaggcgttcg ggctgcccgt gcccgtgccc gagcggaggc ggcaggtccc
     1381 ggagtggtgg cggggccggc tcgggccccg cggcgcggcc ttcgcctacg ggctggggct
     1441 cggggtcggg ttcttgacgt acctgcggca cgggacgctc gtagccgtgt ccgcggtggc
     1501 gctggcgtcg ggcgatccgt ggatcggggc gctcgtgctg gcgccgttcg ggctggcccg
     1561 cggcctgtcg ctgaccgccg tggcgtcgac acggacctcg gacgaggtcc gaggggtgat
     1621 cgatcggctg gagcggatcg cggtgtcggc cctcccccgg ggggcgaacg cggcggcgct
     1681 cgtggtgctc tccgctgtgg cgctcggcgc cgccgcgttc gcccccctcg acgcggcctc
     1741 ggtcggcgcg ctcgccgccg ggatcgtggc ggcggtcttc gcctgggccg tcgccgggaa
     1801 gctcctccac tggaccgtct ggcgagggac actccccggc taccggctgg gcccgatgga
     1861 gccggtcgcg gcggtgggcg cgccggcgct ggaggcggtc gtcgtcgtgc tcgtgctggc
     1921 cggcctcgcc gccgccgcgg gcgcggtggc cctggcgctg ctggccgccg cctccgcggc
     1981 gctcctgcga gcccgcggcg gcggcgaggc cccctgcgcc tgcttcggac ggaggcgggt
     2041 gcggagcgtg cgctcgctcc tcatcaggaa cggggcgatc gcggccgcgg ccgcggcctc
     2101 gctctgggcc gagagccgtc cgccgtccct gccgcccgcg gaccccctcc ccctggcgct
     2161 cgccctgatc ggggccgcgg ccgcgatcgc cctcggcgtc tcggcggccc ggtggctcag
     2221 gcccccggca gcggccggag gaacatctcc ccgggcccgg gcgacgagtc caggcacacc
     2281 cgccggcacc tcgggccggt gaacatcgtc tgggcgctcc accggtagga gaaccggtcc
     2341 ggccccaccg tgaccttgtg gagggccacc cgcacggccg ccccccgctt cccccgcttc
     2401 caggcccgca ccttgccgat ctgccgctcc cgtcccgcgg ggctcacgcg gaagaggcgc
     2461 ccgatcatcc gccggcccgt ggaccggatc accgcgacgt ggtcgggctg ggggcctccc
     2521 cgggcatcga tccacaccag gaggtatgcc ttgtcccagg cgagcttctt cgtccagcga
     2581 gggcgcgtcc gcaggttcca cgtgagctgg gacgtcccct tcgggccccg gacccgcttc
     2641 aggtcgagcg gccctcgcac gtcgcgcggg tcgaaggggt tgggcgcggc ctcgcacgcc
     2701 gtcctcgtcc cgccgtcggc cccgagcagg caggtgtcgg cgcccgggcc cccgtcgatc
     2761 gcgtccgacc ccgcccctcc gtcgaggtga tcgccgcctt ccccgcccag gagggcgtcg
     2821 tctccaccct cgcccaggac cacgtcgttc cctcccccgc cggagagcgt gctgccctcc
     2881 gcggagccga cgaggagatc gtcgtgcgcc gatccggtcg cgtcctcgat ctccgcgaga
     2941 acgtccgcac cctccccgtg agccgtcccg gcggccaggt cgacccgcac accgccgaga
     3001 gacgccgaga agtcggcggt gtcccggccc gcgcccccca ggagctcgtc gtcgccgccc
     3061 cggccgcgca ggacgtcgtc gccgcccagg ccgcagatca cgtccggccc ctgcgtgccc
     3121 tggagctcgt cgtcgccctc agtgccctcg atcgtgcacg gctggccggt ggcctcgacg
     3181 acgagggcgg cctcgtgggc tccggccacc acccccgaga ccgccgccga gccctcgagc
     3241 gctccgggct tcaccggact ggcctcgacg gcgacggtca cggtctcgcc gggcccgacc
     3301 gaccccagcg cgcaggccac gggggggccc gggacgcacg accccacctc gggcagcgcg
     3361 gacgcgagga cgaggcccgc gggcagggtc acctcgagca tcgcctccgg ctcggcgtcg
     3421 gggccggcgt tcgtgacctc gatcgcgagg gagacgggcg agcccaccgg cgaggggccg
     3481 ggcgctcgga cggccaggcc caggtcggcc cgggcggcgt gggcctccga ggcgagcggg
     3541 aggcctgcga gcgccgcggc cgacagggcg atcgcgagcg ccgcggctcg gttccgggac
     3601 atcgcgccgg aacctaccgc acggccggag gcctgtcacg gtgtgccagg atgggtgccc
     3661 gtgaacctgc tcgacgcggc gatcctcgtc ctgctggccc tctcggtcgt gaacggattc
     3721 cggagaggcg cgatgctgca ggtgatcacg ttcgggggcc tcctcgtcgg gctcgtcgtg
     3781 ggcgcggtcc tggccccgca gctcgcctcg ctcgccgacg atcccgcgtg gcagtcgctc
     3841 atcgcgctcg gctcgctgct cctgatcgcc gggctgttcg acgctgcggg gtgggcgatc
     3901 gggacccggg cctggacggc ggccagacgg agccacctcg gcgtcgtgga ctcgtgggca
     3961 ggcgtgggcg tagccgccgt ctccacgctg ctcgtggtct ggttcctcgc cttcaacctg
     4021 gtccagggtc cgatcccggc gctgtcgcgc gagatccggg cgtcctcgat cgtccgcggg
     4081 atcgacgcga tgctgccgcg cccgccctcg atcctggccc aggtccggag cttcctcgat
     4141 cgcttcggct tccccgaggt cttccagggg ctgcctccgg ccccggccgg cccggtggag
     4201 gggcccagcc gcggagaggt ggggcgggct gtggacgccg ccgccccgag caccctgcgc
     4261 gtcgtgggga gggcctgcga ccggatccag gcggggaccg ccttcatcgt ggccgacggc
     4321 tacgcggtga cgaacgcgca cgtggtcgcg ggggtggacg ccccgcaggt ccagcagcag
     4381 agcggcggct cgatcgacgc cacgaccgtg ctcttcgacc cggacctcga tctggcggtg
     4441 ctgcggatcg ccacgacacc cgcggccccg ctcgacctgc tggctgggaa cctggaccgc
     4501 ggccagggcg cggccgtgct cggctaccca cagggcggcg ggctgacggt gggcccggcg
     4561 gccgtgcgac gggtcatgca ggccgtggga cgggacatct acgggcggga caccgtgtcg
     4621 cgcgaggtgt acgagctcca ggccgaggtc cggccgggga actcgggcgg ccccttcgtc
     4681 acggcggacg gcgacgtggc gggcatcgtg ttcgcggcgt cggtcggcaa cgacgacgtg
     4741 ggctacgccc tcacgtcgac cgaggcgctg ccccgggtcg agcgcgggat cggccggacc
     4801 gagcccaccg acaccggccc ctgcctccgc tgacccccta ctcctccatg acgacgcgga
     4861 cgtcgccctc cttggaggcc agcgtgaagg cggtgacctc accctcgcgg cggaacgtca
     4921 gcgagagcgt gctctccccc acgcggaggt tccgcagctc gacctcgttc agccaccgag
     4981 ggagatgcgg cttgttgatc gtgagcaggt tctcgtcggc ccgagccgag accccgagca
     5041 tcgcctggag caggaggaac ggcgcgccgg ccgcccaggc ctgcggcgag cacgccaccg
     5101 ggtactggac gggccggttg ggagagcgcc gcacgaaccc gcagaagagc tccggcaggc
     5161 gcatgtagtc catcgtgatc gcagcctcga acatcgcggt ggccacccgg ttcgtggcct
     5221 ccacgaagcc gtaccgcttc aggcccgccg cgatcagcgc gttgtcgtgg ggccagaccg
     5281 agccattgtg gtagctcatc gggttgtagg cccgctccga cttgctcaac gtccggatcc
     5341 cccaccccga gaacatgtcg ggggcgaaga gccgctgggc gatggcctcg gccttcgccg
     5401 gctcgacggc atcgcagtag agcgcgtgcc ccgggttcga cgtgatcgca cgcacctggc
     5461 gcttcttcgc atcgagcgcc tccgcgaagt acccctcgtc ctccatccag aactcgtcgt
     5521 tgaatcgctt ccggaggacg gtggtctcgt ccctgagggc gaccgcccgg gcgtggtcgc
     5581 cgagcgaggc gaagaggccg gccatcctga ccttcgccat gtacacgtag gcctggacct
     5641 ccacgagggc gatcgggggc ttggccagcg tcccgtcgcg gtgcaccacc gagttccacg
     5701 agtccttcca cccctggttc tcgaggccgc ggtccgaccg gcgcaggtac tcgacgaagc
     5761 cgtccccgtc gaggtcgccg tggtcgtcga tccaggccag cgccgcctcg gccgccggca
     5821 tcagctcgcg tacgagttcg gtgtcggccg tccatcggtg gtactgggcc aggagaatca
     5881 ggaaccacgg cgtcgagtca accgagccgt agtacggcgt gtgcgggatc atgccggccc
     5941 cggccagctc gccccgacgc atctcgtgga ggatcttccc gggctccgcg tcccgccagt
     6001 cgtcccgctc ccgagcctgg agcgacgcca gcagcgccag ggtttcgcgg gccagctgcg
     6061 ggttcaccat caggagctgg tgcgaggtga tgagcgcgtc gcggccgaac ggcgagacgt
     6121 accaggggat gccggccgcc agggtcgctc cgccgtcggc gctcatccag agggcccgca
     6181 ggtcccggag cccccgacgg agcagctcgt tgaagagctc gttgtcggtg cggatcctcg
     6241 tggactcgcg ctcccacgac tcgtaggagc gccgcagctc gtggatcgtg gggtcgtagc
     6301 cgcgcgccgg gagctcacgg cccgggccca cgatcggctg gacctgcagc gacaggaccc
     6361 gggtctcgcc cgacgggatg ctgagcccga acgtggcctc cacgacgtcg ccccgcacct
     6421 ccatccggtc gggctcggcc ccgaacgcga tgcgcgtcgt ccgctgcaca tcgtcctccc
     6481 ccacgtacgt gaacgagatc gaacggcctt cggccttggg gggctcgtgg tgcccgcgcc
     6541 gctgccggcg caggccccgg acctcgaaga tgtcggcgaa gtcggcgccg aacgtgtacg
     6601 tcacgtccac ctccaccgcc cgggcgttgt agttcttgat cctcgtcttc tcgaacaggc
     6661 ggtcgccgac ggcgcggatc cgccggacgt tgaggctctg ctgggcggcc gccacccgcc
     6721 cgtcgccgtc gtagagcgcg gggttcgtca tgtcgatgtg ggccatgtac gcgcgctcgg
     6781 ccgaggagga gagcaggatc gggtcccgcc ccgacagctt gaagcggaag tacgagagga
     6841 acctcgtgtc gaggtggtag aagcccaggc cgagctcgtg ccggtcgtcg aggtgcccct
     6901 cggagtcggc gtagaggaac gtcgctccct ccttcgtggc cagcaccttg ctcgggaggt
     6961 cggtgacgat ctcgggcgca ccctcgttca ggatgcgttc gagggcgtcc tcgaagtggg
     7021 gggaatcggg tggctccggg ccggaggtca tctccgcggg agtgtaaaga ggacctgcgc
     7081 ccggaacaag ggccgggcag cctaggctgg ctcatccccc agccgtcttc ccccatgccg
     7141 cgagtcagcc gggacagagt ggagcgtacg gacgatcggg caggagcgcc cgccattcgt
     7201 ccctgctgag gctgcggccg gcgacgagac aggcgtgccg cttccaggag gccgtatcga
     7261 ggtcccagat gaacgcggcg tcggtgatgt ggacgacggc gagacgacgt ccgtcggcgg
     7321 tgaaggcgac gttcgccggg aagtccgtcg ccgggaaggt gccgatcctg tctcgcgagg
     7381 ggtagcgcca cacccccacg gctccggtgg agtcggccgc ggccacgaga gagccgtcgg
     7441 ggctgacgtc gacgctgatc acctcctgga ggcccgtgag gcctgaaccg tccgggcggc
     7501 gggacgcgat gtcccagaag gtgacgttgc cgtcgaagtc accggccacg agcgttccgc
     7561 cgtccgggga gaaatcggtt tgcagcacca gggagtcgag atcgggagag aggatccgcc
     7621 gccacgtggc cacctcccat acacccacgt cgctgacgcc gtccgcgcaa cacgtcacga
     7681 tggagaggag ccgtccgtcg gggctgaagt cgatctccgc ggcgccaccc tcgagatcga
     7741 gctccttcag cggccggcga gctctcgcgt cccacacgat ggccctcgtt cggaggccgg
     7801 ccggatcggg gctgtgcgtg gttgcgaggc gcgtgccgtc cgggctgaac gtcacccacc
     7861 acccgcctcc ctgcggcttc ggccgaggac gcagcctcgc tatcgtcgaa cccgtctcga
     7921 cgttccgcag gaccacgatc cccgacgccc ccgtggacgc gatcgttcgc ccgtcggggc
     7981 tgaacgccag cccgttgacg ggtacgtctc ccgtggcggc cagcctcccg agctcttctt
     8041 gggttgcgac gtcccagagg aggatcgttc cgtccgcctg tgggacggcg aggacatcgc
     8101 cctggggact cagcgccgag atctgcgagt actcggccgc cccgatcccg cggtccaacc
     8161 gtcgcccgaa gcgccttcgt cccccgaggt cccagacgat gacgagcccg tcatgaccgg
     8221 aggtgtacag ggtctcgcca tccggatcga cgaccgaatc cgagaccgtc ccggcgtgcc
     8281 caggaagggt catcgtgggc ccgccggctg gcgcccaggg agggagctcc gattcgaggt
     8341 cccacaccat cgccgctcga tccgcgctcg tcgtcgcgat caaggtcccg ttcgggtcga
     8401 agacgacggc ggtgatcggg gcgtcgtgcc ctccgacgag atcacgctgc tctccggact
     8461 tgagatcgag cacaatgagc gagccatcct ctccgccaat ggctgctcgg cttccatcag
     8521 ggctcatccc catgggacct ccgacgtcga agctccggac gggctcgcgg tgcgcgacgt
     8581 cccacagcac cgtcgtcccc cgatccgtgg aggtcactag gcgagcgtca ccggggagga
     8641 aggcgaggtt gagcgtgggt ccctcaaggt cccgacgcga gagcgacagc cggccctgga
     8701 gagcaccggt cctcgcgtcg aagaggagga ccgccttgcg gggaggcggt ccgaaggggt
     8761 tggcgccctt cgggaactcg tgcgtggcgg ccgcaagggt ggcgccgtcg tgcgacaccg
     8821 ccaccgactc cgcgcccacc ccgggaggca gctcgatgcg ctcgatcgtt cgatcggtca
     8881 cgtcgaccag gtcaaggtgc gatggtgcat ctccaggccc ctgggtggcg acaaccagag
     8941 tccggccgtc cgggctgaac accagcgatc cagaagaacg gtcgaccgtc ccctcgaggt
     9001 gtaccggctg acccagccgt ccgcgggtct ccgcgtcgaa gaaggcgagg tcccccgtcg
     9061 agctcgcgac cgcgaggatc cgaccgtcgg gcgagatcgc gatgctctcc aggaagcggc
     9121 ccggcccctg catgatgccg atcgcgtcgg ggctgcgaac cagacctgcg aggagggatc
     9181 gccgagactc cgtggagtcg tccagtcgaa cggccgcgag cgccaacagc agtgagcggt
     9241 ccagccgttc ctcggagagc gcctgtgtgg cgagcctacg agcatcggcc agggtcgcct
     9301 gcgcctcggc agcggatcgc tcctccgccg ctcgatcgcg ctgcacgagc gcgaccaacg
     9361 ccagggtcga ggcgacgagc gcggctgcga tcgagccccc gacgatgccg agccggcgtc
     9421 gccggcgtct cgccgatcgc cgatcctcgc gggcacggcc gtcggcgagg tacgcgcgct
     9481 cctcctccgt gagggtcagt ctcgtggtcg ccgcccaccg ctcgtactcc tccagacggc
     9541 ctccccccag gaggtagtcg tccaggcggc cggcgtctct ccattcggag accgctgcgg
     9601 ccaggcgccg gtggaggagc aggtcctcgc ggcggctctc gatccagcgg cgaagggagc
     9661 cccattcgcg gaacagcgcc tcgtgggcga cctcgaccgt ggggcctcgc gtcacgggat
     9721 cgcggtcgaa ggagagcaga cgatgggctc cgtattgccg tacgacccga tcgatggcct
     9781 tggggtcggc tccgaggccg gcgagctcgc gacggctcac acggcgccgg gcgtcttggg
     9841 cgtgttcatc gacggagacc agccggagga agacctgttt ggcaacctcc tggccggccg
     9901 gagggagtga gccgtagacc tcctccgccc ggcgtgccag cgctccgagg actccaccgg
     9961 tttcacggta ggcggcagcc gtgagggtcc ggccgtcgcg gcgtgcgaag agctcggtca
    10021 gggcgtgttg gaggagcggc aggccgccgg gctgatccac gacgtctcga acgatggcgg
    10081 cgaccagccc gggctcgtac tcgacgccga cccgctcggc cggcccggac accgctcggg
    10141 ccagctcctc gggagatgga accgtcacgg gaaccaggcc tgccctgacc agccctccga
    10201 acgcatggtg ctcgaggggg cggtcgaagt agtcggcccg aagcgtcagc acgatgcgaa
    10261 cacgacctcg agcgtcctca gccagcgcga ccaggccgtc gaggaacatt tggcgcagat
    10321 ccgaacgggc ggtgaggctg aacagctcct cgaactggtc gaccacgagg accagctcac
    10381 tgtcatctcc cggcaggatc tgcttgatcg ctcgaagcat cccccgatcg tcggccatga
    10441 gctcggccag cagcccgtcc ggcgggtcga tcgcgatccg cagcagggcc acctccagct
    10501 cctcgaacgg ataggaaccg gggaacatct cggtgacgag ccaggcccga gacccgggca
    10561 ccgctcccgc tcgaagcgcg gggagcaggc ccgcccgcac cgccgaggac ttgccgcttc
    10621 cactgggacc cacgacggcc acgagtcgac gggtcgcgac ggcctccacc agccgatccg
    10681 tcaaggcctc tcgtccgaag aagtcgagcg cgtccgcctc cccgaacggc tgcaggccct
    10741 tgtacgggtt ccggaccggt tccgagtcct ccacgagctc gaccatcgcc accgcgtcgg
    10801 tgcccgcggc acggcgcagt tcacggagca catcctcgac ccgagcctga cgccggtgcg
    10861 ggtcggggtc ggtggcccga gccagcaccg actgaagccc aggaggcagg tccggccgag
    10921 ccgctgcgag tccggaggcg gggttttcgg tcggccccgt tgctcccgtg agggcttcca
    10981 cggccagcac ggcaagcgcg tagagatccg aggccggcga gagaggctgc cctcgtcgct
    11041 cctcggggct gacggaggcg ggcgccgcgg tctgcggggc ccccgccgcg tcgacggccc
    11101 gtggcacgat ggcgaagtcc gtgaggagcg cgttgccctc atcgtccagc aggacgttgg
    11161 aggtcttgat gttgccgtga accactccgt gccggtgggc ggaggcgatg gcggcgccaa
    11221 cctgctcgag catccggagg gtcgccgcca ggctccacgg accgtgccga agggcacgcc
    11281 ggacgctccc ccccggcacc cacggggtca ccaggtaggc gcctcccgga tcgcgccatg
    11341 agtcgaggag cggcagagcg ttcgcgtggc tgggctcggc gatgcgacgg gtgcggggct
    11401 cgaaccctcg gacgaaggag ggggcgttgg aatactccgg ccggataacc ttgatcgcga
    11461 cctcacgccc gaccgaggcc tgataggcgc ggtacgtgac gccgaagtcc ccggagccca
    11521 cccgctctcg aagctcgaag ccgcgaacca cgtggaagcc cgccgggcct gcttcctccg
    11581 tcgcaccgtg ggcgggccgg tcctctgggc ccgtggccgg gacgagctcg gggtcttgga
    11641 cgaggatctg ccggtggagg gaccggagct cgggggacgc gtcgatcccg agctcgtcgg
    11701 cgagcaagtc gcgggtacgc tcgaacgcgc gtagcgcgtc ggcctgccgg ccacagcggt
    11761 agagcgccag catgtggagc tcccgcagcc gctcacggta gggatgctcc cgggtgagcg
    11821 cctcgagctc ggcgacgagc tcgacgtgtc gcccgcactc gaggtccgct cgcacgcgat
    11881 cctccaccgc gctcaggcgc gactgctgga gtcgggagac ctccggaagc atcccgggtg
    11941 catcccccag atccgcgtag ggctccccgt tccagagggc cagggcctct cggagcagct
    12001 ccgctgcgcc ggccggggcg tcgccgagcc gctcccgagc ggcctcgacg agcgaatcga
    12061 atcggtgggc gtcgaccgcg tcggggtcga ccgcgagtcg gtatcccttg ccctcgcggg
    12121 cgatctgatc gtcgaggagg ctgcgcagct ccgagatgta ggtctggagg gtgtggcggg
    12181 cgccctccgg tgcgtcctcc ccccagacgc cgtcgatcaa ccggtcctcg gcgacgacat
    12241 ggttggcgtc ggcgagcagc aacgccagca cgagccgctg cttcgggccg cccaactcga
    12301 cgaagccccg atccgtcgat gcccggatcg gcccaagcac ctggtaccgc accaatcgaa
    12361 ccatactctc gcgccgctcc cttgggaatc cggacccggc ccgggtctga ggcagacacg
    12421 tatcgacgcg gccgcgccaa cgttgcgcga cgtcgctcaa ggacggctca agaacacctc
    12481 ggtgcgacca tccgggcctc cttgattcgc tctggagagg accttgagcg cctccctcga
    12541 cgctctcccc tgacgcaggg cggcgtcgag gggccgatcg gcggcccgga aaggaggcga
    12601 catgcgggag ctgctcgttg ttcctctggt gctggcggcg ctcctcgtgg cggctgcccc
    12661 ggtgggcgcg gagcagccac tttcgttcga gcgcttcgag ttcgaggcgg gtccgttccc
    12721 agacccgttc atcctcgagg tgtgcggcct cgaggtgctg acctcgtccc aggcgaaggg
    12781 cacgatcacg ctgttcaccg acgggacgct gaaggagcac gtcaatctgg tcatcacctc
    12841 gaccaacccg gacaacggcg tcaccacgac cgagcgcgac gcgttcacgt tcttcagcga
    12901 gccgttcgag gcggtgatcg acgaggaggc cgggacgatc acgcgcgtgt tccgggaggc
    12961 tttcgtcggc ctgcccttcc ggcagatgct ccccggcggc ggggtcttga tccgggacgc
    13021 tgggaaggtg gtgctcctcg tgacgatcgt ggaggacctt cggaccgggg aggagctctc
    13081 cgtcgaggtc gaggagctcg aggtccgcgg gccgcatccg gcaggccccc tcgacctgga
    13141 ggagaggtac aggcccctct gtgacgcatt ggccggggag gggaggtgag gctcgacagc
    13201 cagggtgaga acgcttccag atgacccgaa caggaggtgt ggagatgaga aggctgaaga
    13261 gatcgttcgc tcggatcgcg atggtcgcga ccgtggcggg ggcgctcctg accggatcgc
    13321 ccgcgggagc cacggagccg gtgggtgggt gcgctccacc cttcgaagag cccatcctga
    13381 tcgaggacct cgcgttgccc gtgctcgtgg agttcgcgca ggcgatcgac acggctacgg
    13441 gggcgaaccg tgacggctac gtgtgcttca tgcccctggc gacgggcaac cagacagggt
    13501 ggctgcagct cgtgaacatc ctcgacaacc gcctcccacg gtgagg
//
LOCUS       DASUTZ010000114         3895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1325621, whole genome shotgun sequence.
ACCESSION   DASUTZ010000114 DASUTZ010000000
VERSION     DASUTZ010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325621"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2550)
                     /locus_tag="VF097_05205"
     CDS             complement(<1..2550)
                     /locus_tag="VF097_05205"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX6262228.1"
                     /translation="MSDSPRFHPLVEGWFAEALGEPTATQAEGWRRIAAGRDTLVAAP
                     TGSGKTFAAFLWAIDELVRQAEADALGDETSVVYVSPLKALGNDIQKNLREPLDAIRD
                     RAAEQGLELPDIRVLVRSGDTPQSERAQMIKRPPHILVTTPESLFIMLTAERSRGFLG
                     SVRTVIVDEIHAVAGDKRGAHLALSLERLDRLAGQPLQRIGLSATQKPIAEVARLLVG
                     NGRLRDDGSPDCEIVDAGHRREWDLSVEIPDAPLGPIATHELRAEMYDRIAELAKQHR
                     TTLVFVDTRRLAERVTHALEERLGQEHVAPHHGSLSREIRLRAEQGLKEGSLRVVVAT
                     ASLELGIDIGHVDLVCHVGAPRSIATLLQRIGRSGHFLGGTPRGAIFPLTRDELTQTA
                     AAIRAARAGELDRVIFPREPLDVLAQQIVAIAAAEEVREEDVYALVRRAFHYKDLSRE
                     DFDEIVEMLSEGVAGRHGRRSAHLHRDRVNGVLKGRRGARLAAITSGGAIPDIGDYDV
                     VEEPAGTFVGTVNEDFAIESMAGDIFLLGNTSWRIRRVEAGKVRVENAHGLPPTIPFW
                     VGEAPGRTEELSTAVSDLRAEVASRLEDPEAAVRWLVEETEVPEAGAQQIVEHLAEGV
                     ALLGAIPTKDTVVAERFFDESGGMQLIIHAPFGARINRAWGLALRKKFCVQFDFELQA
                     AATDDAILLSLGEQHSFPLESVAGYLKPLTLRNTLIQAMLPSPMFANRWRWNATRALA
                     LLRHSGGRKVPVPIQRMRAEDLLSAVFPEQVMCQDNRMGDVVPPDHPIVNETVKDCLH
                     EAMDLDGLAEIVERIGSGEIRFVAAETPAPSVFSHEVLNAGPFAFLDDAPLE"
     gene            complement(2588..3352)
                     /locus_tag="VF097_05210"
     CDS             complement(2588..3352)
                     /locus_tag="VF097_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX6262229.1"
                     /translation="MLDAELGLANELIDRAASIAMPLFRSRPEVRMKDDETPVTQADI
                     RIEEMVRELVANRFPSDAVLGEEGGGSLRDASRVWVVDPIDGTKNFAAGIHVWGTLLA
                     LFEEGAPVLGVVGAPALNERYEAVRGQGATLNGSLISVSEVDSLERAALCHYATEAWL
                     DGPHEAALRDLDRAAYRSVGFTDFWGHCLVARGSVEVALEPQLRIWDWGALKVLVEEA
                     GGRMTTFQGEPVSDRTSVISTNGRLHETVLEILATG"
     gene            3497..>3895
                     /locus_tag="VF097_05215"
     CDS             3497..>3895
                     /locus_tag="VF097_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262230.1"
                     /translation="MLRIGSGRMQLKRAWVALATAVVVTGAMAGGTAMGAPPGSTKLV
                     ITAVTDQGTGLPVPIAGQPFSVAVELRDQSDTLVTTSKKIDVTLSVSEGMLQGTTTGT
                     ILKDQSGTTISGVIYPDTANVTLFASAKQIE"
BASE COUNT          575 a         1420 c         1329 g          571 t
ORIGIN      
        1 ctcgagcggc gcgtcgtcca ggaaggcgaa cggccccgcg ttcagcacct cgtgagagaa
       61 gaccgagggg gccggggtct ccgccgccac gaaccggatc tcgccgctcc cgatcctctc
      121 cacgatctcg gccagcccgt cgaggtccat cgcctcgtgc aggcagtcct tcacggtctc
      181 gttcacgatc ggatggtcgg gcgggacgac gtcgcccatc cgattgtcct ggcacatcac
      241 ctgctcgggg aagaccgcgg acagcaggtc ctcggcccgc atccgctgga tcggcaccgg
      301 caccttccga cccccgctgt gtcgcagcag ggccagggct cgcgtcgcgt tccagcgcca
      361 ccggttggcg aacatcgggg aggggagcat cgcctggatc agcgtgttcc gaagggtcag
      421 cggcttcaga tacccggcca cgctctccag cgggaagctg tgctgctcgc cgagcgagag
      481 caggatggcg tcgtcggtgg cggcggcctg cagctcgaag tcgaactgca cgcagaactt
      541 cttgcggagc gcgagccccc aggcccggtt gatccgcgcg ccgaacgggg cgtggatgat
      601 cagctgcatc ccgcccgact cgtcgaagaa ccgctccgcc accaccgtgt ccttcgtggg
      661 gatcgcgccc agcagcgcga cgccctcggc caggtgctcc acgatctgct gggcgccggc
      721 ctcggggacc tccgtctcct cgacgagcca ccggacggcc gcctccgggt cctccaggcg
      781 tgaggccacc tcggcgcgca ggtccgagac cgcggtcgag agctcctcgg tcctccccgg
      841 agcctcgccg acccagaagg ggatcgtggg ggggagcccg tgggcgttct ccacccggac
      901 cttccccgcc tccacccggc ggatccgcca cgatgtgttg ccgagcagga agatgtcgcc
      961 ggccatcgac tcgatggcga agtcctcgtt cacggtgccc acgaaggtgc cggccggctc
     1021 ctcgaccacg tcgtagtcgc cgatgtcggg gatcgcgccc ccggaggtga tggccgcgag
     1081 ccgggccccg cggcgtccct tgaggacgcc gttcacccga tctcggtgga ggtgcgccga
     1141 gcggcggccg tgacggccgg ccaccccctc cgacagcatc tccacgatct cgtcgaagtc
     1201 ctcgcgcgag aggtccttgt agtggaaggc acgccgcacg agggcgtaga cgtcctcttc
     1261 gcgcacctct tcggcagcgg cgatcgccac gatctgctgg gccaggacgt cgagcggctc
     1321 tcgtgggaag atcacccggt ccagctctcc ggctcgcgcg gcgcggatcg cggcggcggt
     1381 ctgcgtgagc tcgtcccggg tcagggggaa gatggcgccc ctcggcgtgc cgcctaggaa
     1441 gtggcccgac cgtccgatcc gctggagcag cgtggcgatc gagcggggcg cgcccacgtg
     1501 gcatacgaga tccacgtgcc cgatgtcgat ccccagctcg agggaggcgg tggccacgac
     1561 cacccgcagc gacccctcct tcagaccctg ctccgcccgc aggcggatct cccgcgacag
     1621 cgagccgtgg tggggggcga cgtgctcctg gcccagccgc tcctccaggg catgggtgac
     1681 ccgctccgcg aggcgcctcg tgtccacgaa gacgagggtc gtccggtgct gcttcgccag
     1741 ctccgcgatc cggtcgtaca tctcggcccg gagctcgtgg gtggcgatcg ggccgagcgg
     1801 cgcgtcgggg atctccacgg acaggtccca ctcgcggcga tggccggcgt ccacgatctc
     1861 gcagtcgggc gagccgtcgt cgcgtaggcg cccgttccct acgaggagcc gcgccacctc
     1921 cgcgatcggc ttctgggtcg ccgacagccc gatccgctgg aggggttgcc cagccagccg
     1981 gtccaaccgc tccagggaga gagcgaggtg ggcccctcgc ttgtctccgg ccacggcgtg
     2041 gatctcgtcc acgatcacgg tgcggaccga gcccaagaac cccctcgacc gctcggcggt
     2101 cagcatgatg aagagcgatt cgggcgtggt cacgaggatg tgcggagggc gcttgatcat
     2161 ctgcgcccgc tccgactgcg gcgtgtcgcc cgaccggacc agcaccctga tgtcggggag
     2221 ctcgagcccc tgctcggccg ctcgatctcg gatcgcatcc agcggctccc ggaggttctt
     2281 ctggatgtcg ttccccagcg ccttcagagg cgagacgtac acgaccgacg tttcgtcccc
     2341 gagcgcgtcc gcctcggcct gccgcacgag ctcgtcgatc gcccagagga acgccgcgaa
     2401 ggtcttcccg gagccggtcg gggcggcgac cagcgtgtcc cggcccgcgg cgatccgtcg
     2461 ccaaccctcg gcctgcgtgg cggtgggctc gcccagagcc tccgcgaacc agccctccac
     2521 cagcgggtgg aagcgggggg agtcgctcac gcgctcaacc taccatccgg caccgacacg
     2581 gaagccgtca gccggtcgcc aggatctcga gcaccgtctc gtggagtctc ccgttcgtgg
     2641 agataaccga ggtccggtcg ctcaccggct ccccctggaa cgtggtcatc cggccgcccg
     2701 cctcctcgac gagcaccttc agcgctcccc agtcccagat ccgcagctgg ggctcgagcg
     2761 cgacctcgac cgagccgcgg gccacgaggc agtgccccca gaagtccgtg aacccgaccg
     2821 agcggtacgc ggctcggtcg aggtcgcgca gagcggcctc gtgcggaccg tcgagccagg
     2881 cctcggttgc atagtggcag agggccgctc gctccagcga gtcgacctcc gagacggaga
     2941 tcagcgagcc gttcagcgtc gcgccctggc ctcggacggc ctcgtagcgc tcgttgaggg
     3001 cgggtgctcc cacgacgccc aggaccggcg ctccctcctc gaacagcgcg aggagggtcc
     3061 cccatacgtg gatccccgcc gcgaagttct tcgtgccgtc gatggggtcc acgacccaca
     3121 cgcgagacgc atcgcggagc gagcccccgc cctcctcgcc cagcacggcg tccgacggga
     3181 agcggttcgc gaccagttcg cgaaccatct cctcgatccg gatgtcggct tgcgtgactg
     3241 gcgtctcgtc gtctttcatc cgaacctcgg gccgggatcg gaacagcggc atcgcgatcg
     3301 aggcggcgcg gtcgatcagc tcgttggcga ggcccagctc cgcgtcgagc atcccggcat
     3361 catagcggcc gaagagggcc ttccccctcg cggcgcctcg tgctagggta ggtccttcgc
     3421 aggaccccca tcccgcccac acgtatgggg agacccgcgg acaggtcacg ggcacgacaa
     3481 cgggagggga tgggagatgc ttcggatcgg atccggcagg atgcagctca agcgcgcctg
     3541 ggtcgcgctg gccacggcgg tggtcgtgac cggtgccatg gccggcggga cggcgatggg
     3601 cgcccctccg ggatccacga agctcgtcat caccgccgtc acggaccagg gcaccggcct
     3661 cccggtgccg atcgcgggac agccgttctc ggtcgccgtg gagctgcgcg accagtcgga
     3721 caccctggtg acgacctcga agaagatcga cgtcacgctc agcgtgagcg aggggatgct
     3781 gcaggggacc accacgggaa cgatcctcaa ggatcagagc ggcacgacga tctccggcgt
     3841 gatctatccc gacacggcca acgtcacgct cttcgcctcc gccaagcaga tcgag
//
LOCUS       DASUTZ010000115         3011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_937543, whole genome shotgun sequence.
ACCESSION   DASUTZ010000115 DASUTZ010000000
VERSION     DASUTZ010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937543"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VF097_05220"
     CDS             <1..228
                     /locus_tag="VF097_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 2'-N-acetyltransferase"
                     /protein_id="HEX6262231.1"
                     /translation="HIAARYQLGALGGDPAFYVPLGWRVWEGPTFVRTEEGLVRTPEE
                     DGSVLVRPTPSTPALDLTAPISCEWRRGEVW"
     gene            complement(194..1321)
                     /locus_tag="VF097_05225"
     CDS             complement(194..1321)
                     /locus_tag="VF097_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005438716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6262232.1"
                     /translation="METPPADPVLHASQDGEGGGRLRIAIVAPPWYSIPPSGYGGIES
                     LVSWLTERLVQRGHDVTLIAAAPSRTSATFRATLAEPPSHRMGEALPEIVHTARTRTI
                     LEEIGPDVVHDHSTAGPLLAGIGAPTILTAHGPVRGDYGAYYRSVAHGVGLVAISDAQ
                     RELAPEIPWFGMVHNAIPVGDYTFRDTKEDFALFLGRMSPEKAPHLAIEACREAGQPL
                     VLAAKCREPDEIEYFEAEVRPRLGPDTEWLGEVDDKTKKDLLSRARCLAFPIQWDEPF
                     GLVMAEALASGTPVVACRRGSVPEVVGDGVTGFVCDELDQLPDAIRRASELDPGACRE
                     RASSMFDLDVMVSGYERVYRKAIGQADDPPVTRPRRDATRS"
     gene            complement(1337..1831)
                     /locus_tag="VF097_05230"
     CDS             complement(1337..1831)
                     /locus_tag="VF097_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020416864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262233.1"
                     /translation="MDKRLLSIYLNDHLAGSRGAVGLARRSMKNNEGSPFGDDLMRVA
                     REIEEDRATLEELMEMAGVGRAPMKDGAVWLAERLARLKLNGRLLAYSPLSRLEELEV
                     LRLGIEGKRSLWRNLSEIRAEVEGWEGVDFESLIERAEGQIEVLESHRIATVRQAFFQ
                     PTTV"
     gene            1888..2490
                     /locus_tag="VF097_05235"
     CDS             1888..2490
                     /locus_tag="VF097_05235"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX6262234.1"
                     /translation="MDGGSGRVDGDTRILTVPNVLSFVRIAAIPVFVMLLLDEGTRLW
                     GFLVLGVVQGTDWVDGYVARRYRQVTELGKVLDPLADRLAVGAALITFVVADLFPLWA
                     ALLVLVRDGLVLVAVGVLALLGAPRIEVRRIGKVATFVLMWAIPMIAWGNAGFLLDDV
                     VLVHGWIWFGVGYLEYLVATGAYVVDVRGALRARPPTLGG"
     gene            2526..>3011
                     /locus_tag="VF097_05240"
     CDS             2526..>3011
                     /locus_tag="VF097_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX6262235.1"
                     /translation="MKAVIMAGGEGTRLRPLTSNQPKPMIPIVGRPMMEHVLHLLERH
                     GFDESVVTVQFLASVIRNYFGDGDDMGIDLAYVTETEPLGTAGSVKNAEHFLDETFLV
                     ISGDAVTDIDLSQAVAFHREKGSAVTVVLKRVENPLEFGIVIADDEGRIERFLEKPGW
                     GE"
BASE COUNT          407 a         1052 c         1064 g          488 t
ORIGIN      
        1 cacatcgccg ctcgctacca gctcggggcg ctgggaggcg atcccgcctt ctacgtgccc
       61 ctgggatggc gggtctggga gggtcccacg ttcgtccgaa ctgaggaggg gctggtgcgc
      121 acccccgagg aggatgggag cgtgctcgtt cgcccgacac cctcgacgcc ggcgctcgac
      181 ctcaccgcgc cgatcagctg cgagtggcgt cgcggcgagg tctggtgacc gggggatcgt
      241 cggcctgccc gatggccttc cggtagacgc gttcgtagcc cgagaccatc acgtccaggt
      301 cgaacatcga ggaggctcgc tcccgacacg cgcccggatc cagctcgctc gctcgccgga
      361 tcgcgtccgg cagctgatcg agctcgtcgc agacgaaccc ggtgacgccg tcgcccacga
      421 cctcggggac cgatcctcgc ctgcaggcca ccacgggggt gccgctggcg agcgcctcgg
      481 ccatcacgag accgaacggc tcgtcccact ggatggggaa ggcgagacat cgcgctcggg
      541 agagcagatc cttcttcgtc ttgtcgtcga cctccccgag ccactcggtg tcggggccga
      601 gacgaggccg gacctcggcc tcgaagtact cgatctcgtc gggctctctg cacttcgctg
      661 cgagcacgag gggttggccg gcttcgcggc acgcctcgat cgccaggtgc ggggccttct
      721 ccggcgacat gcggcccagg aacagggcga agtcctcctt cgtgtcgcgg aacgtgtagt
      781 cgcctaccgg gatggcgttg tggaccatcc cgaaccaggg gatctccggc gcgagctcgc
      841 gctgcgcgtc ggagatcgcg acgagtccga cgccgtgtgc caccgagcgg tagtaggcgc
      901 cgtagtcccc ccgcacgggc ccgtgggccg tgaggatggt gggggcgccg atcccggcca
      961 ggaggggtcc cgccgtcgag tggtcgtgga ccacgtcggg tccgatctcc tccaggatcg
     1021 tccgcgtcct cgcggtgtgg acgatctcgg gcagagcctc tcccatccgg tggctcggag
     1081 gctccgccag cgtggcccgg aaggtcgcgc ttgtcctcga gggcgcggcg gcgatgagcg
     1141 tgacgtcgtg gccccgctgg acgagccgct cggtcagcca ggacacgagg gactcgatcc
     1201 cgccgtagcc gctgggcggg atgctgtacc agggtggcgc gacgatggcg atgcggagac
     1261 ggcctccgcc ctcgccgtcc tgtgacgcgt ggagcaccgg atcggcaggg ggagtctcca
     1321 tcgtcatcgt ctcgtctcag accgtcgtgg gctggaagaa ggcctgccgc accgtcgcga
     1381 tgcgatggct ctccagcact tcgatctgtc cctccgcccg ctcgatcaac gactcgaagt
     1441 ccacgccctc ccagccttcg acctcggcgc ggatctccga gaggttcctc cacagagagc
     1501 gcttcccctc gatccccagg cgaaggacct cgagctcctc gagcctgctg aggggcgagt
     1561 aggcgaggag ccgcccgttc agcttgaggc gggccaggcg ctccgccagc cagacggcgc
     1621 cgtccttcat cggcgcgcgc cccacgccgg ccatttccat cagctcctcg agcgtcgccc
     1681 ggtcctcctc gatctcccgg gccacgcgca tcaggtcgtc cccgaaggga gagccctcgt
     1741 tgttcttcat gcttcgccgg gccagcccga cggcgccgcg ggagccggcc aggtgatcgt
     1801 tcaggtagat cgagagcagc cgcttgtcca tgccgcgggt ccttccccat cccgcgccgc
     1861 tccaatccgc ggggctagac tctccccgtg gacgggggga gcgggcgagt cgacggcgac
     1921 acccgcatcc tcaccgtccc gaacgtcctg tcgttcgtgc gcatcgccgc gatccccgtg
     1981 ttcgtgatgc tgctcctgga cgaggggacg aggctctggg gcttcctcgt gcttggggtg
     2041 gtccagggaa ccgactgggt ggacgggtac gtcgcgcgtc ggtaccggca ggtgaccgag
     2101 ctggggaagg tgctggaccc gctcgccgac cgcctcgcgg tgggggcggc gctgatcaca
     2161 ttcgtcgtgg cggacctgtt cccgctgtgg gcggccctgc tcgtgctcgt gcgcgacgga
     2221 ctcgtcctcg tggcggtggg ggtgctggcg ctcctcggcg cgccccggat cgaggtgcgc
     2281 cggatcggaa aggtcgccac cttcgtgctg atgtgggcca tccccatgat cgcctggggg
     2341 aacgccggct tcctcctcga cgacgtggtg ctcgtccacg ggtggatctg gttcggggtg
     2401 ggctacctcg agtacctcgt ggccaccggc gcgtacgtcg tcgacgtccg cggggcgctg
     2461 cgggcccggc cgcctacgct cgggggctga cccgcgggcc cggggcccgt gggtatcatg
     2521 gcgcgatgaa ggccgtcatc atggcgggcg gcgagggcac ccgcctccgt cccctcacca
     2581 gcaaccagcc caagcccatg atccccatcg tggggcgccc catgatggag cacgtgctcc
     2641 acctcctcga gcggcacggc ttcgacgagt cggtcgtgac cgtgcagttc ctggccagcg
     2701 tgatccggaa ctacttcggc gacggggacg acatggggat cgacctcgcc tacgtgaccg
     2761 agacggagcc gctgggcacg gccggcagcg tcaagaacgc ggagcacttc ctcgacgaga
     2821 ccttcctcgt gatctccggc gacgcggtca ccgacatcga cctctcgcag gccgtggcct
     2881 tccaccgcga gaagggctcg gccgtgaccg tggtgctgaa gcgcgtggag aaccccctgg
     2941 agttcgggat cgtgatcgcc gacgacgagg gccggatcga gcgcttcctg gagaagccgg
     3001 gctggggcga g
//
LOCUS       DASUTZ010000116        21550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1456578, whole genome shotgun sequence.
ACCESSION   DASUTZ010000116 DASUTZ010000000
VERSION     DASUTZ010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 21550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21550
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1456578"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1995)
                     /locus_tag="VF097_05245"
     CDS             complement(<1..1995)
                     /locus_tag="VF097_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX6262236.1"
                     /translation="MDPHDLLATLQSEAPDRLVHLRTLPERRPQLARLPDLPEPLPAR
                     LGNLGIEGLYPHQAEALDLLEEGRNVILSTGTASGKSLVYNVHLARTLLREPRSTGLY
                     LFPTKALARDQLRQVRDLRLPQIKASVYDGDTPRAERPLIRRTANLILSNPDMLHASL
                     LPDHARWADFLLRLAVVVVDEAHVARGVFGSHVAMVLRRLRRLVAKYGGDPRFVLASA
                     TIGNPADLAERLTGVPFAEVTEDASPAGEKLFALWNPPLVDEEEGVRKGAVSEAAGLM
                     ARLVDRDERTIAFARSRRAAELLAEIARREVRAARRDRIKTYRAGYLPEERRRLERAL
                     AEGELLGVASTNALELGIDIGSLDAAILAGYPGTRASMWQQAGRAGRRSGASIAFLVA
                     QDDPLDQFLVSHPQDLFDKPPEDAVVDPTNPYVLAPHLACAAREHPLAPEELSFFGEA
                     APDAVASMLEDGRLVERRGLLYHRGGPSPHREVDIRSASGSLYLIVDVDTGAVLGNVD
                     ESRAFFQVHPGAIYLHQGEQYEVVTLDRERKAALVRPSDADWFTQSRDVTDIEVLRVR
                     AETKVGSTPAFFGDVRVTTQVVGFQRRLHITGEVLGVVDLDLPPQTLVTRALWWAVPD
                     ETIERAGVTPSALPGAAHAAEHAAIGLLPLVATCDRWDIGG"
     gene            2069..3058
                     /locus_tag="VF097_05250"
     CDS             2069..3058
                     /locus_tag="VF097_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX6262237.1"
                     /translation="MSVAAAEQAQARFASFDVPMWVWAVLVGAIVTLLLIDLLIVHRT
                     PHVISFKEAAIESAVWVAIGISFVFVLLWWQGGQAAGEYFAGYLIEKSLSIDNVFVWA
                     VLLTYFHVPREYQFRVLFWGVFGALVMRAVFIFAGVALLERFEWILYVFGAFLVYTAI
                     RIARHSGAEVHPERNPVLKLVRRVIPTTHYYDGQKMLTRQEGRLMATPLLVVLILVEA
                     TDVLFAVDSVPAILAVSREPFIVWSSNAFAIMGLRALYFLLAGMADRFRFLNVGLGVI
                     LGFVGVKMLLADVYHVPVWASLVVIAAVLAVTVWLSLRASRREEEETAEPRSR"
     gene            complement(3055..3993)
                     /locus_tag="VF097_05255"
     CDS             complement(3055..3993)
                     /locus_tag="VF097_05255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX6262238.1"
                     /translation="MRRGAALLVALVVGLGAWVAVAAVFAGPRPAGAQTPAVEISRAQ
                     DARYTPSVGGNRPIFILAIGSDARPDEQVDRRLADSLHIVAVNPGQGTASILGFPRDS
                     YVPIPGFGEAKINESLYNGGPELVVETVEGLTGIQMDYWVLTSFRGLIGMVNGIGGLE
                     IDVPYPMDDFYSGAQFEAGEQVIDGTQALAFARNRHDTPEGDFSRSENQGRLLLAALE
                     QLRAQVRRDPTVLFQWIAVGLRSVQTDLSFDEVFDLMMTALSIDPEGVTNAVVPGGTG
                     SAGAASVVILSGDAPAIFEDLGDDGILQNPPPPPGG"
     gene            complement(3990..4649)
                     /locus_tag="VF097_05260"
     CDS             complement(3990..4649)
                     /locus_tag="VF097_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262239.1"
                     /translation="MPQRAHAASGRALPWILVGLLVLVASGAGAWALLRDGGRGDGEE
                     DLPVPDLVWTVGRVRSTSVDADASQGDLEEARDAIADLLDGLYTTAFVDPAKWEGGAF
                     PELSSYFAGRAAARADRDRDSLTLGEDAARIERVEPTRATLRLTFLVDPQAAPYAAVA
                     TAGFRADGRTTDGGAVAIVHRGRYTVRRIEGEWRIIGYDVEGSLESVADPGPGTPGAT
                     P"
     gene            4782..7055
                     /locus_tag="VF097_05265"
     CDS             4782..7055
                     /locus_tag="VF097_05265"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX6262240.1"
                     /translation="MVVLAQEGGPISAEFGSFEDAVLWIILGISVLALVVAYVLRRGV
                     LAAPEGTEKMREIATAIQEGSSAYLNRQFRTVGMFLVVLTVAIFFLLPVPEKAETSAI
                     AIRLGRSVAFILGAGFSAVTGFAGMWLAVRGNVRVANAAKETGLKRAVVIAFRTGGVA
                     GMFTVGLGLLGAVVILLIYKQDATSVLVGFGFGGALLAMFMRVGGGIYTKAADVGADL
                     VGKVEKGIPEDDPRNAATIADNVGDNVGDCAGMAADLFESYEVTLVASIILGAAAFAD
                     SPEGAIVGVMFPLFVRAIGVITSIVGIYAVTPRSDTEHGMRAINRGFFISALLSAAAV
                     YFVADAYVNDLRVFWAVVIGLLLASVIHVLTEHYTSTKRRPVKEVAESSQTGAATTIL
                     SGFSVGLESTVWAILVIAGTIFSAYLLGNDIAERLYFISLTGMGMLTTVGVIVSMDTF
                     GPVADNAQGIAEMSGEFEGRPAEVLEGLDAVGNSTKAITKGMAIATAVIAATSLFGSF
                     EEALKEAGYSFLGIRVDQPEVLIGLLVGGAVSFLFAALAIRAVGRAAAQVVVEVRNQF
                     RDHPGIMGGTEKPDYGRVVDICTRTSLRELLTPGLLAVLTPIIVGFAFEAEALGAFLA
                     GVILTGQLLAVMLSNSGGAWDNAKKLVEEGLYGGKGSESHKATVIGDTVGDPFKDTAG
                     PALNPLIKVMNLVALLIAPLVVTMADDTVTRIAIAGGGAVILGLAIWHSKRQRGPVLV
                     PSEPPSPEAATVTTESG"
     gene            7060..7413
                     /locus_tag="VF097_05270"
     CDS             7060..7413
                     /locus_tag="VF097_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX6262241.1"
                     /translation="MFGRLLHRFAESDEDRLANEVRAWADSMPETIRISDVRERARVR
                     LAGVVRRITLHPGPEGAESLSAVLSDGTGEIVARWTGRGEIGGLRLGSRVLLEGVVAR
                     ERGRLQIVNPSYEFA"
     gene            complement(7415..8104)
                     /locus_tag="VF097_05275"
     CDS             complement(7415..8104)
                     /locus_tag="VF097_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011905383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX6262242.1"
                     /translation="MRVTIAGAGNVGRHLAYDLAQRGHDVTIIEQETSILERAKDALP
                     AGTMFVLGDACEPWVLEKAELSSADVIVAATGDDEDNLVISLLAKQEHGVPRVVARVN
                     HPKNEWLFTEQWGVDVSMSPPHVLTALVEEAVTVGDLVRLLKLEGGRVTLVELTIDES
                     APIAGRPLYELRPPPDSAIVAVLREGHVVVPQPETRLVAGDEIMAISTLEAEDDLREA
                     ILGPTPHSEAR"
     gene            complement(8104..8823)
                     /locus_tag="VF097_05280"
     CDS             complement(8104..8823)
                     /locus_tag="VF097_05280"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX6262243.1"
                     /translation="MTARQRRPRSWGLATGGVPLPAVHIVIMGCGRVGAELTVQLSKM
                     GHSVSVIDKRSEAFDRLPPGFDAKKIVGIGFDQEVLDEAGIKEADAFIAVSNGDNSNI
                     LSARIAREHYKVPRVIARIYDPRRAEIYERLNIPTVATVRWAAKQIQFLLFHGKEELR
                     DTIAGGSLLIVQVPVPEHLVGQKIEEIRVEGKIEVISVDRGGAGFIPVPGSTFQEGDV
                     AHAIVHRESLDEFEALLEVTE"
     gene            8835..11177
                     /locus_tag="VF097_05285"
     CDS             8835..11177
                     /locus_tag="VF097_05285"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX6262244.1"
                     /translation="MATATRALRAGVKRVLLGRALHSGKLEHQLLPKTLALPVFASDP
                     LSSNAYATEEMMLVLALAGAASFRLMLPIALAIALVLIIVITSYRQTVRAYPRGGGSY
                     IVARENLGTIPGLVAASAILQDYVLTVAVSITAGTVAITSAAPELVPYRMVIAVALIA
                     FVALANLRGVKEAGTLFAIPTYGFVLMVYITLITGFLGCLDGCPRAESADLHLEEAQA
                     LTLFLILRAFASGSTALTGVEAIADGVQAFRRPQARNAAATLGAMGLMTIGMFIGITV
                     LARGLHVRVGEEFLEKSVLAQIGETVFQGGAMFYILQVFTALILILAANTSYQDFPRL
                     SAILARDRFMPRQFMNRGDRLVFSNGIVVLSVLAALLVWIFDANLNRLIQLYVVGVFT
                     AFTLSQSGMVRRWLRRKEPGWRRGAIINGIGASATGVVLIIVTVTKFTHGAWIVVAAM
                     PIIVFLLLSVYRHYMRVGQLLRARGITLETEVENTFLALVPDFGHATREMVSWLRAVR
                     PESDRVLPLYLGDMPLERARVEWERIAPRLGQMDRLDEEPGLQMRTLRRFVRGLRRGP
                     NDFTTIIVPEEVSTGLLFYILRRTRSFRVKAAFLLEPGVVVTDIPLLPEERETAEERA
                     RAGRPLEPSRNVCLVPVSAVHDATMRALAYAQSLNPAHLEAVFLAGEPEEADELLAAW
                     REHDLDIPLSVVEAPFRDYGPPMLEEIRRHTSREDTVVTIVLPEYVVDKWRHALLHNQ
                     RALFFKRKLLFEPWVVAVSIPYQIGAAEAPPAFSPARAEA"
     gene            11180..11731
                     /locus_tag="VF097_05290"
     CDS             11180..11731
                     /locus_tag="VF097_05290"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX6262245.1"
                     /translation="MERADAVALVREKTEKETTVRHLISVEGVMRALARRFDEDEETW
                     GLVGLFHDLDQDQTHDDLERHAHQAAEWLREARVDERIVTGVLAHAHDEYRTDLMTSA
                     IVHADALAGLLVAAALVRPQKAEGMKVSSVRKKLKEKAFAPGVNREEITSVEESIGLP
                     LEDFIAAGIEGLQEVAPQIELSG"
     gene            complement(11721..12377)
                     /locus_tag="VF097_05295"
     CDS             complement(11721..12377)
                     /locus_tag="VF097_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX6262246.1"
                     /translation="MLEWLEHLIVDVIGTYGYFAIFVLMMLESALIPIPSEVTMVFGG
                     FLVARGDLDFIAVGTIGAVANVAGSWLAYYVGRVGGRPLAERWGRYVFLREKELDRAD
                     EWFASRGELTVFVTRLLPVIRTFISFPAGVARMPFWRFTLYTFLGCLPWSFALAWAGV
                     LLGENWEVFLRHSRPVVIGVGVATAALIGWWLWRRAAARRQEQERDEPEPAEEDELTR
                     "
     gene            12485..14830
                     /gene="topA"
                     /locus_tag="VF097_05300"
     CDS             12485..14830
                     /gene="topA"
                     /locus_tag="VF097_05300"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX6262247.1"
                     /translation="MANKLVIVESPTKAKTLERYLGPGYRVKASYGHVRDLPKSKMAV
                     DTERNFEPEYEVPDAKKKVVAELKRAHKQADELWLATDFDREGEAIAFHVAELLGVSP
                     EEAKRVTFTEITQSAVREAFAEPRTIDLQLVEAQQARRILDRLVGYSISPLLWKKVRP
                     GLSAGRVQSPALRLIVEREREIAAFQPKEYWSVDARLAPNGDEDAFLAGLWQIGEEKL
                     AANPDKKGLVLESEEVAREHAGNLERATYRVTEVRRSERKRNPSPPFTTSTLQQEAAR
                     KLGFTSRKTMRMAQQLYEGVELPGEGQVGLITYMRTDSVTIAEQALAEIADVVRLQYG
                     KEYALDKPRRYKKKQRGAQEAHEAIRPTSAARLPDVVQAHLDRDQARLYRLIWQRTMA
                     SQMAPAVFDQVSVDIEAAADDAPRYLLRGTGQTMKFDGFRRAYFEGRDDDVDEDAESR
                     LPELTAEQALRLLEVLPEQHFTQPPPRYTEASLIKKLEELGIGRPSTYSSIVSTLLDR
                     EYVRLEERRFYPEDVGEVTTDVLVDRFGDHFLDYEFTARMEGELDDIAEGKMRWNQVL
                     DEFHAPFERKVEKAEEELERPEEEIDELCPRCPEEGREPGKLVIKLGRRGKFVGCKNY
                     PECRYTRDVDGQERPEPELLEETCPECGKQLVKRWGRYGPFIGCSGYPDCRYIKREEK
                     KTGVTCPKCGEGELIEKRARRRRGNVFYGCNRYPDCDFTVGQRPLTEPCPECGALMTA
                     QKDGSGKCQNGHVIEGAAERSAPASEEKEPEATGSGSAEAG"
     gene            14832..16832
                     /gene="tmk"
                     /locus_tag="VF097_05305"
     CDS             14832..16832
                     /gene="tmk"
                     /locus_tag="VF097_05305"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX6262248.1"
                     /translation="MVDYADEIAALRGTKPATFGSLFKNRSFFRLWRAMLVSSLGDWT
                     GFVAITALVTRIGGPRAGYAVTGVMLARLLPSLIFGPVAGVFVDRFDRRKLMVIADIG
                     RGAGYVSMAFVGELWLIYALSFSVECLSLLWTPARDASIPNIVSRRQLSNANSMGLFT
                     SYGTLPLGGILFTALTALATGIGGVIEYFGDRPESLALLLNSVTFAFSAWMIWGLQLP
                     ERSRRAQGRLRPAQAWDDLREGIRFLRAHAFQRTMTIGVVLAFTGVGSVIALGPIFAQ
                     NTLGAGQAGWGIVVTSVGLGLAAGMISVGLVERVIDKETLFPLAMIGAAGLLVVLSLM
                     PNIALAAMLTAVMGSVAGLAWVAGYTLLHENVGDEFRGRTFATLTILSRLGLLASLAG
                     FPALAQTIGDYSIGIGDAEIDLAGTRLALWVGAAVVLVGGVSARRGLRRGRRSRAQPL
                     QLRPQLRRGERPGTFIVFEGVEGSGKGTQVELAKDFLEGEGYEVLVTREPGGTPLGEG
                     LREVLLGDQPLDARAEALLFAAGRAQHAVAVIRPALEKGKVVLCDRYVDSSLAYQGVG
                     RDLGEPDVLALSAWATQGLFPDLVILLNIDPEEGLERSGGDDRFESEGDEFHEKVAEA
                     YLRIADEHPERVVVVDALGTPEEVHGRVRDAIRRFLKGQEEA"
     gene            16829..17962
                     /locus_tag="VF097_05310"
     CDS             16829..17962
                     /locus_tag="VF097_05310"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HEX6262249.1"
                     /translation="MSGVPVLASVPGQDEAIAFLERAAERPGHAYLFAGPEGSGKKLA
                     AAAFTAALLCERGGCGECRDCRLALEDHHPNVAVLEPEGRDIRVGEGADDFGTARWLV
                     ARAHRTAPEPGRKVFRIEQADRMTEEAADVLLKVVEEPPPETVFLLMSARPHELTETI
                     RSRCQTVAFHPLAEGFVVETLTAEGVSEGRAMLASRLAGGNVGRARRIARDPEGLAFR
                     DEALAALREVGGDPRGALAGAQRLVDAAADYKKGLGRVLDEELEPLLDEETGRVAEQF
                     RGLAKRIDERHKRRERRAEREFLDWSLLALEAWYRDALLISTGGDPSWVINMDLDWSA
                     DGVSSASAAAAIEALEEARAALADDTNLNAKLVVERAFLRIAS"
     gene            complement(17966..19867)
                     /locus_tag="VF097_05315"
     CDS             complement(17966..19867)
                     /locus_tag="VF097_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262250.1"
                     /translation="MSELDDRIREGLERLAPRADGSGAIDDVVRRKRRYRTARRARAG
                     ALAVAVVLGSAAGVWGLSRAFNLDESRPAVPGGRDGLIAFSVQTEGGWDIYVVNPDGT
                     GLRPLAVGPHDEIHPVWSPDGSRLAFVTERGGAGNLHIVRADGTGRRESFGSPVEMFA
                     PAWSPDGRAISMADADGSLYTHEPFLPSGGTRTISHPSRVRAMGSPSWSPDGGRIAFV
                     GGGDQPQIYLWDLGAHEVAQLTEMMERPTSPVWSPDGSRIAFLNRWAREGCSGVDLDT
                     SQDGSFGLHIVEPRTALTTRLFDRACHGVPAWSPDGSRIAFVGGDVPALTVTVLDINA
                     PENPVMAIDVEGEIPAIAWSPNGNLVAYSTPGSVRVAPIGGEPREIVSGIGEVGGISW
                     QPVPGPVVGPTPSPSPSAPDVLPCGNDGVPFLAAYRPIDETETDVDGDGETARVRLIA
                     DEERPPECRYFLYVEPPVGTPLSAVVPPMEGVPSVPEILMAAEIDGEPGHEIVVDIGG
                     PGHPHRTGVVFTMDQGEIAPMQTYDPLSRNPRGPIPTPFVPLGGEFAAGVDCAAPNLR
                     AAMIVITVGTFADGGQDDTRLDITRTFYRADGAFFEQVERDTYTVSIGEERERWPETD
                     DDPFRSCPR"
     gene            complement(19854..20348)
                     /locus_tag="VF097_05320"
     CDS             complement(19854..20348)
                     /locus_tag="VF097_05320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6262251.1"
                     /translation="MSETEIRAFLKEEYPRVVAAIALVGGSRVAAEDAVQEALARAWE
                     RTERGESIETLAAWVTTVAMNLSRSRWRRAMAERRAWARLAAVRSDERPAEDVVDLER
                     ALRGLSRRQREVVVLRYYLGEDVAGMARILRVSEGTVKTTLHRARKALAEALNDREDD
                     RVGA"
     gene            20475..20942
                     /locus_tag="VF097_05325"
     CDS             20475..20942
                     /locus_tag="VF097_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262252.1"
                     /translation="MSEQMAEVAGRVFVAFLFVLGGLVVWRWLRSGSPWARRISLALS
                     IVAGGIMLVAAVQAALGSGDESREPTGQAEVVFLGVPGYELWPDLELERAAESQLQDD
                     PALHDKWADVSIRKAYRGNGLVAQAMAIELRPGLGDTEGLLDDVARGPVAGRR"
     gene            20950..21129
                     /locus_tag="VF097_05330"
     CDS             20950..21129
                     /locus_tag="VF097_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262253.1"
                     /translation="MERRMRGVSIRVSPRFRGARGSVYVMTWLRGDVIVTVSGQRAGA
                     VRRFAAAVIEADREA"
     gene            21161..21235
                     /locus_tag="VF097_05335"
     tRNA            21161..21235
                     /locus_tag="VF097_05335"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21196..21198,aa:Thr,seq:cgt)
     gene            21347..>21550
                     /locus_tag="VF097_05340"
     CDS             21347..>21550
                     /locus_tag="VF097_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX6262254.1"
                     /translation="MAIGLPAPGSATQGPPEPMPGLIDAAEIARDERGIAHITARNDH
                     DLFFLQGWVHAGDRLFQMDVSRRQ"
BASE COUNT         3134 a         7376 c         7681 g         3359 t
ORIGIN      
        1 gccgccgatg tcccaccggt cgcaggtggc gacgaggggg aggaggccga tggccgcgtg
       61 ctctgcggcg tgcgccgccc cggggagggc cgagggcgtc acccccgccc gctcgatcgt
      121 ctcgtcgggg acggcccacc acagggcgcg ggtcacgagc gtctgcgggg ggaggtcgag
      181 atccacgacg cccagcacct cgccggtgat gtgcaggcgc cgttggaacc ccaccacctg
      241 ggtcgtcacg cggacatccc cgaagaacgc cggcgtcgag cccaccttcg tctcggcgcg
      301 gacgcggagc acctcgatgt cggtgacgtc ccgcgactga gtgaaccagt cggcgtcgct
      361 ggggcggacg agggcggcct tccgctcccg atcgagggtg acgacctcgt actgctcccc
      421 ctggtggagg tagatcgcgc cggggtggac ctggaagaag gcccgggact cgtccacgtt
      481 gcccagcacc gcgccggtgt cgacgtcgac gatgaggtag agggatcccg aggccgagcg
      541 gatgtccacc tcgcggtggg gcgacgggcc ccctcggtgg tagaggagcc ccctgcgttc
      601 gacgagacgc ccgtcctcga gcatcgaggc caccgcgtcg ggggcggcct cgccgaagaa
      661 ggacagctcc tcgggggcga gcgggtgctc ccgggccgcg cacgccaggt gcggggccag
      721 cacgtacggg ttggtggggt ccacgaccgc gtcctcgggg ggcttgtcga agaggtcctg
      781 cgggtgggac acgaggaact ggtcgagcgg gtcgtcctgg gctacgagga aggcgatcga
      841 ggccccggag cggcggccgg cccgcccggc ctgctgccac atcgatgccc gggtgcccgg
      901 gtagccggcg aggatggcgg cgtcgagcga gccgatgtcg atgccgagct cgagggcgtt
      961 cgtggaggcc accccgagca gctccccctc cgccagggcc cgctcgaggc gtcgccgctc
     1021 ctcggggagg tagccggccc ggtaggtctt gattcggtcg cggcgcgcgg cgcgcacctc
     1081 tctccgggcg atctcggcga gcagctccgc cgcgcgccgc gaccgcgcga acgcgatcgt
     1141 ccgctcgtcc ctgtccacga gccgcgccat cagcccggcc gcctcggaga cggccccctt
     1201 gcgcacgccc tcctcctcgt ccacgagcgg ggggttccac agggcgaaga gcttctcacc
     1261 cgcgggggag gcgtcctcgg tcacctcggc gaacggcacg ccggtgaggc gctcggccag
     1321 gtccgcggga ttcccgatcg tggccgaggc caggacgaac ctcgggtcgc ccccgtactt
     1381 ggccaccagc cgccggaggc gccgaaggac catcgccacg tgcgagccga agaccccgcg
     1441 agcgacgtgg gcctcgtcca ccaccacgac tgcgaggcgc aggaggaagt ccgcccaccg
     1501 tgcgtggtcg gggaggagcg aggcgtgcag catgtccggg ttggagagga tcaggttggc
     1561 cgtcctcctg atcagcggcc gctccgccct cggcgtgtcg ccgtcgtaga ccgatgcctt
     1621 gatctgcggg agccgcaggt cccgcacctg tcggagctga tcccgggcca gcgccttcgt
     1681 ggggaagagg tagaggccgg tgctgcgggg ctcgcgcagg agtgtccggg ccaggtgcac
     1741 gttgtagacg aggcttttcc cgctggccgt ccccgtggag aggatgacgt tccggccctc
     1801 ctcgagcaga tcgagggcct cggcctgatg agggtagagc ccctcgatcc ccaggttgcc
     1861 gagcctcgcg gggaggggct cggggaggtc gggcagccgc gccagctggg ggcggcgctc
     1921 gggcagggtc cgcaggtgga cgagccggtc gggcgcctcg ctctggaggg tggcgaggag
     1981 gtcgtgtggg tccatgcccg ccgatgttct cacgccccgc ccgggcgtga tgtcggttct
     2041 cttcgggtat cctccgcccg tgggcgccat gagcgtagcc gcggcggagc aagcgcaggc
     2101 tcgtttcgcc agcttcgacg tcccgatgtg ggtgtgggcc gtcctcgtcg gggccatcgt
     2161 caccctgctc ctgatcgacc tcctgatcgt ccaccggacg ccgcatgtga tctcgttcaa
     2221 ggaggcggcg atcgagagcg cggtgtgggt ggcgatcggg atcagcttcg tcttcgtcct
     2281 gctctggtgg cagggcggac aggccgcggg ggagtacttc gccggctacc tgatcgagaa
     2341 gagcctctcg atcgataacg tcttcgtctg ggccgtgctg ctcacctact tccacgtacc
     2401 gcgcgagtat cagttccgcg tcctgttctg gggggtgttc ggggcgctgg tgatgcgggc
     2461 cgtcttcatc tttgcggggg tggcgctgct ggagcgcttc gaatggatcc tgtacgtgtt
     2521 cggggcgttc ctcgtctaca cggcgatccg gatcgcgcgg cactcgggcg cggaggtcca
     2581 ccccgagcgc aatccggtgc tcaagctcgt gcgacgcgtc atccccacca cgcactacta
     2641 cgacggccag aagatgctga cccggcagga ggggcggctg atggccacgc ccctgctcgt
     2701 ggtgctgatc ctggtcgagg ccaccgacgt gctgttcgct gtcgactcgg tccccgcgat
     2761 tctcgccgtg agccgtgaac cgttcatcgt ctggtcctcg aacgccttcg ccatcatggg
     2821 gctccgggcg ctctacttcc tgctggccgg gatggccgac cggttccgct tcctcaacgt
     2881 ggggctgggc gtgatattgg ggttcgtcgg ggtgaagatg ctcctggccg acgtctacca
     2941 cgtcccggtc tgggcctcgc tcgtggtgat cgccgcggtg ctggcggtga ccgtctggct
     3001 ctcgctcagg gcttcccggc gcgaggagga ggagaccgcg gaaccccgga gccgctaacc
     3061 gccgggcggt ggcggggggt tctggaggat cccgtcgtcg cccaggtcct cgaagatcgc
     3121 aggcgcgtcg ccggacagga tgacgacgct cgccgcgccc gcgctccccg tcccgccggg
     3181 cacgaccgcg ttcgtcacgc cctcggggtc gatgctcagg gccgtcatca tcaggtcgaa
     3241 gacctcgtcg aacgagaggt cggtctggac gcttcgcagg cccacggcga tccactggaa
     3301 gaggaccgtg gggtcccgac ggacctgcgc ccgaagctgc tcgagcgcgg cgagcagcag
     3361 ccgcccctgg ttctcggagc ggctgaagtc gccctcgggc gtgtcgtgcc ggttccgagc
     3421 gaacgcgagg gcctgggtgc cgtcgatcac ctgctccccg gcttcgaact gcgcccccga
     3481 gtagaagtcg tccatggggt acgggacgtc gatctccagc cccccgatcc cgttcaccat
     3541 gccgatcagc ccgcggaacg aggtaaggac ccagtagtcc atctggatgc ccgtgaggcc
     3601 ctccaccgtc tccacgacga gctcggggcc gccgttgtac agggactcgt tgatcttcgc
     3661 ctcgccgaac cccgggatcg gaacgtagga gtcccggggg aagcccagga tcgaggcggt
     3721 cccctggccg gggttcacgg cgacgatgtg gagcgagtcc gcgagccgcc ggtccacctg
     3781 ctcgtcgggc cgggcgtcgc tgccgatcgc gaggatgaag atgggacggt tccctcccac
     3841 cgacggggtg tagcgagcgt cctgtgcccg agagatctcc acggccggcg tctgggcccc
     3901 cgccggtcgg ggccccgcga agacggcggc cacggcgacc caggccccga gccccacgac
     3961 gagcgcgacg aggagcgcgg ctccgcgcct cacggcgtcg cccccggcgt gccgggcccc
     4021 gggtcggcga ccgactccag cgacccctcg acgtcgtatc cgatgatccg ccactccccc
     4081 tcgattcgcc ggaccgtgta ccggccccga tggacgatcg ccaccgcgcc gccgtcggtc
     4141 gtccgcccgt cggcccggaa gccggccgtc gccaccgccg cgtagggagc ggcctgggga
     4201 tccacgagga acgtgagtcg cagggtggcc ctcgtcggct cgacccgctc gatccgggct
     4261 gcgtcctcac ccagcgtgag gctgtcccgg tcccgatcgg cgcgcgcggc cgccctcccg
     4321 gcaaagtacg agctgagctc ggggaacgcg cccccctccc actttgcggg gtccacgaac
     4381 gcggttgtgt acaggccgtc gaggaggtcg gcgatcgcgt cccgcgcctc ctccaggtct
     4441 ccctgcgagg cgtcggcgtc gaccgaggtg gagcggaccc gaccgacggt ccagacgagg
     4501 tcggggacgg gcaaatcctc ctccccgtcc ccccggccgc catctcgaag cagggcccag
     4561 gccccggcgc ccgaggcgac caggacgagg aggcctacga ggatccaggg aagggcccga
     4621 ccggatgcgg cgtgtgctcg ttgaggcaaa ccgcccggca gggtagcaga cctcggcgcg
     4681 ggagcaagcc ggttggtcgc gcccggtccg acccgctagg ctatggcgcg ctgagcgcct
     4741 ccttcgcgcc ggccacaatt cactccaagg aggaacaccc catggttgtg cttgcccagg
     4801 aaggcggccc gatctccgcc gagttcgggt cgttcgagga tgcggtgctg tggatcatcc
     4861 tcgggatctc ggtgctggcg ctcgtcgtgg catacgtgct gcggcgaggc gtccttgctg
     4921 cgcctgaggg caccgagaag atgcgagaga tcgccaccgc gatccaggag ggcagcagtg
     4981 cctacctcaa ccggcagttc cgcacggtcg ggatgttcct cgtggtgctg acggtcgcga
     5041 tcttcttcct cctccccgtg ccggagaagg ccgagacctc ggccatcgcg atcaggctgg
     5101 gccgatcggt cgcgttcatc ctgggagccg gcttctcggc cgtcaccggc ttcgccggga
     5161 tgtggctcgc ggtgcgcggc aacgtgcggg tcgccaacgc cgccaaggag acgggcctga
     5221 agcgcgccgt cgtcatcgcg ttccgcaccg gcggtgtggc cgggatgttc accgtgggcc
     5281 tggggctgct aggggccgtc gtgatcctcc tgatctacaa gcaggacgcg acctctgtgc
     5341 tcgtcggctt cggcttcggg ggcgccttgc tcgcgatgtt catgcgggtg ggcggcggga
     5401 tctacacgaa ggcggccgac gttggtgccg acctcgtagg gaaggtcgag aagggcatcc
     5461 ccgaggacga cccccgcaac gcggccacga tcgccgacaa cgtcggcgat aacgtcggcg
     5521 attgcgcggg gatggctgcc gacctcttcg agtcctacga ggtgacgctg gtggcctcga
     5581 tcatcctggg cgcggcggcg ttcgccgatt cgcccgaggg cgcgatcgtc ggcgtcatgt
     5641 tcccgctctt cgtacgggcg atcggggtca tcacgtcgat cgtcggcatc tacgcggtga
     5701 cgccgaggag cgacaccgag cacggcatgc gggccatcaa ccgcgggttc ttcatctcgg
     5761 cgctgttgtc cgcggcggcc gtgtacttcg ttgccgacgc ctacgtgaac gacctccggg
     5821 tcttctgggc ggtcgtgatc gggctgctcc tggccagcgt gatccacgtc ctgaccgagc
     5881 actacacctc cacgaagcgc cggccggtga aggaggtcgc ggagtcgtca cagaccggcg
     5941 ccgccaccac gatcctctcc gggttctcgg taggcctgga gtccaccgtg tgggcgatcc
     6001 tcgtgatcgc cgggacgatc ttctcggcgt acctcctcgg caacgacatc gccgagcgcc
     6061 tctacttcat ctcgctcacc ggcatgggga tgctgacgac cgtcggggtc atcgtgtcga
     6121 tggacacgtt cggccccgtg gccgacaacg cgcagggcat cgcggagatg tcgggcgagt
     6181 tcgaggggcg gccggccgag gtcctggagg ggctcgacgc cgtcggcaac tccaccaagg
     6241 cgatcacgaa ggggatggcg atcgccaccg ccgtgatagc ggccacctcg ctgttcggct
     6301 cgttcgagga ggccctgaag gaggccggct acagcttcct cgggatccgc gtggaccagc
     6361 ccgaggtcct gatcgggctg ctggtcggcg gcgcggtctc attcctgttc gccgcgctgg
     6421 ccatccgagc ggtcgggcgc gctgccgccc aggtcgtggt cgaggtgcgg aatcagttcc
     6481 gcgaccaccc ggggatcatg ggcggtaccg agaagcctga ctacgggcgc gtcgtcgata
     6541 tctgcacgcg cacgtccctg cgcgagctgc tcacccccgg cttgctggcg gtgctgacgc
     6601 cgatcatcgt ggggttcgcc ttcgaggccg aggccctggg cgcgtttctg gcgggggtca
     6661 tcctgacggg ccagctcctc gcggttatgc tctcgaactc cggcggggcc tgggacaacg
     6721 cgaagaagct cgtcgaggag ggcctctacg gcggcaaggg atccgagtcg cacaaggcca
     6781 cggtcatcgg cgacacggtg ggcgacccgt tcaaggacac tgccgggccc gccctcaacc
     6841 cgctgatcaa ggtgatgaac ctcgtggcgc tgctgatcgc accgctcgtg gtgacgatgg
     6901 cggacgacac cgtcaccagg atcgcgatcg cggggggcgg cgcggtgatc ctgggcctcg
     6961 ccatctggca ctcgaagcgg cagcgagggc cggtcctggt cccgtcggag ccgccgagcc
     7021 cggaggccgc gaccgtcacc accgagagcg gctgaggccg tgttcggtcg gctgctccac
     7081 cgcttcgcgg agtccgacga ggatcgcctg gcgaacgagg tccgggcctg ggcggactcc
     7141 atgccggaga cgatcaggat cagtgacgtc cgcgagcggg cccgggtgcg gctggcgggc
     7201 gtcgtgcggc ggatcaccct gcacccgggg cccgagggag ccgaatccct gtccgcggtc
     7261 ctctccgacg ggacggggga gatcgtggcg cggtggaccg ggcgtgggga gatcggcggc
     7321 ctccggctcg ggtcgcgcgt cctgctggaa ggcgtggtgg cgcgggagcg ggggcgcctc
     7381 cagatcgtga acccgagcta cgagttcgcc tgagtcagcg cgcctctgag tggggggtgg
     7441 gcccgaggat cgcctcgcgc aggtcgtcct cggcctccag cgtggagatc gccatgatct
     7501 cgtcgccggc cacgagcctc gtctccggct gcggcacgac gacgtggccc tcccggagga
     7561 cggccacgat cgccgagtcg ggcggcggcc ggagctcgta gagcggtcgg ccggcgatcg
     7621 gggccgactc gtcgatcgtc agctcgacga gcgtgacccg cccgccctcc agcttcagca
     7681 gccggacgag gtcgcccacc gtcacggcct cctcgaccag cgccgtcagg acgtgtgggg
     7741 ggctcatcga gacgtccacc ccccactgct cggtgaagag ccactcgttc ttgggatggt
     7801 tcacgcgggc caccacccgc ggcacgccgt gctcctgctt ggccagcagg gagatgacga
     7861 ggttgtcctc gtcgtctccg gtggcggcca cgatgacatc ggccgaggag agctcggcct
     7921 tctccagcac ccagggctcg caggcgtccc ccaggacgaa catcgtcccc gcggggaggg
     7981 cgtccttggc ccgctcgagg atcgaggtct cctgctcgat gatcgtcacg tcgtggccgc
     8041 gctgggccag gtcgtaggcg aggtgccgcc ccacgttccc ggcgcccgcg atcgtcaccc
     8101 gcatcactcc gtcacctcca ggagggcctc gaactcgtcg agagactcgc ggtgcacgat
     8161 cgcgtgtgcc acgtctccct cctggaaggt cgagccgggc accgggatga agccggcgcc
     8221 gccacgatcc acgctgatca cctcgatctt cccttcgacc ctgatctcct cgatcttctg
     8281 tcccacgagg tgctcgggga ccgggacctg gacgatcagc agggagcccc cggcgatcgt
     8341 gtcgcgcagc tcctccttcc cgtggaagag gaggaactgg atctgctttg ccgcccagcg
     8401 aacggtggcc accgtgggga tgttcagccg ctcgtagatc tcggcccgac gcgggtcgta
     8461 gatgcgggcg atgacccgcg ggaccttgta gtgctcgcgg gcgatccggg ccgagagaat
     8521 gttcgagttg tcgccgttgg agacggcgat gaaggcgtcg gcctccttga tgcccgcctc
     8581 atccagaacc tcctggtcga acccgatgcc cacgatcttc ttcgcgtcga agccgggcgg
     8641 cagccggtcg aacgcctccg aacgcttgtc tatcacggag accgagtgcc ccatcttgga
     8701 gagctggacc gtgagctccg cccccacgcg cccgcatccc atgatcacta tgtgcacggc
     8761 tggtaagggt actccgcccg tggccaggcc ccaactccgc ggacggcgct gtcgagcggt
     8821 catagaatcc ggccatggcc acggcgacga gggcccttcg agcgggcgtc aagcgtgtcc
     8881 tgctggggcg ggcgctgcac tccgggaagc tggagcatca gcttcttccc aagacgcttg
     8941 ccctccccgt cttcgcctcg gatccgctct cctcgaacgc gtacgccacc gaggagatga
     9001 tgctggtcct ggctctcgct ggcgcggcct cgttccgcct catgctcccc atcgccctcg
     9061 cgatcgcgct cgtgctgatc atcgtcatca cgtcataccg acagaccgtg cgtgcgtatc
     9121 ctcggggcgg gggttcctac atcgtggccc gcgagaacct cggcaccatc cccggcctcg
     9181 ttgcggcttc ggccatcctt caggactacg tcctcaccgt tgcggtctcg atcacggccg
     9241 gtaccgtggc gatcacctct gccgcacccg agctggttcc gtatcggatg gtgatcgcgg
     9301 tggcgctgat cgctttcgtg gccctggcga atctccgagg ggtgaaggag gccggaaccc
     9361 tcttcgccat ccccacctat gggttcgtgc tcatggtcta catcaccctc atcaccggtt
     9421 tcctcggttg cctcgacggc tgcccgcggg ccgagagcgc ggacctccat ctggaggagg
     9481 cccaggcgct taccctcttc ctgatcctgc gcgccttcgc ctcgggctcg accgcgctca
     9541 ccggggtgga ggcgatcgcg gacggggtcc aggccttccg ccgtccccag gccaggaacg
     9601 ccgcggccac gctgggcgcg atgggtctca tgacgatcgg catgttcatc ggtatcaccg
     9661 tgctggcccg gggactgcac gtacgcgtgg gcgaggagtt cctcgagaag tccgtcctgg
     9721 cgcagatcgg ggagacggtg ttccagggtg gcgcgatgtt ctacatcctt caggtcttca
     9781 cagccctgat cctcatcttg gccgccaaca cctcctatca ggacttcccc cgcctctccg
     9841 cgatcctggc tcgggacagg ttcatgccgc ggcagttcat gaaccggggg gaccgcctcg
     9901 tcttctcgaa cggcatcgtc gtgctctcgg tcctggcggc gctgctcgtg tggatcttcg
     9961 acgcgaacct caatcgcctc atccagctct acgtggtcgg cgtgttcacg gccttcaccc
    10021 tgtcccagag cgggatggta cggcggtggt tgcggcggaa ggagccaggc tggcgccgtg
    10081 gcgccatcat caacgggatc ggcgcgagcg caaccggcgt cgtgctcatc atcgtgaccg
    10141 tgacgaagtt cacccatggg gcctggatcg tcgtcgcggc gatgccgatc atcgtgttcc
    10201 tgcttctctc ggtgtaccgg cactacatgc gcgtgggtca gctgctccgg gctcgtggca
    10261 tcacgctcga gacggaggtt gaaaacacgt tcctggcgct ggtccccgat ttcggccacg
    10321 ccacgcggga gatggtgtct tggctccggg cggtgcggcc ggagtcagac cgcgtcctac
    10381 ctctctacct cggtgatatg cccctagaga gggcgcgagt ggaatgggag cggatcgctc
    10441 cgaggctggg ccagatggat cgtctggacg aggagccagg tctgcagatg cgaacgcttc
    10501 gtcgcttcgt ccgaggcttg cgacgtgggc cgaacgactt cacgacgatc atcgttcccg
    10561 aagaagtctc cacgggtctc ctgttctaca tccttcggcg tacgcggtcg ttccgagtga
    10621 aggcggcgtt cctcctggag cccggcgtgg tcgtgaccga catcccgctt ctcccggagg
    10681 agcgggagac ggccgaggaa cgagctcgag cgggtcggcc tctggagccc agccgcaacg
    10741 tctgcctggt cccggtctcc gccgttcacg acgcgaccat gcgtgccctg gcctacgctc
    10801 aatcgctgaa ccccgctcac ctggaagcgg tgttcctggc cggtgagccc gaggaggccg
    10861 acgagctcct cgcggcgtgg cgggagcacg atctcgacat cccgttgagt gtggtggagg
    10921 cgccgttccg cgactacggg ccaccgatgc tcgaggagat ccgaaggcac acctcccggg
    10981 aggacacggt ggtcacgatc gtgctgcccg agtacgtggt ggacaagtgg cgtcatgccc
    11041 tcctccacaa ccagcgagct ctcttcttca agcggaagct gctcttcgag ccttgggtgg
    11101 tggcggtgag catcccgtat cagatcggcg ccgcggaggc gccacccgcg ttctcgccgg
    11161 ccagggccga ggcctgagca tggaacgagc tgacgcggtc gcgctggtgc gggagaagac
    11221 cgagaaggag accaccgtcc ggcacctgat cagcgtggag ggcgtgatgc gagcgctggc
    11281 tcgccgcttc gacgaggacg aggagacgtg ggggctcgtc gggctcttcc acgacctcga
    11341 ccaggatcag acccacgacg accttgagcg ccacgctcac caggcggcgg agtggctccg
    11401 ggaggcgcgg gtggacgagc gcatcgtgac cggggtcctg gcccacgccc acgacgagta
    11461 ccggaccgac ctgatgacga gcgccatcgt ccacgccgac gcgctggcgg ggctcctcgt
    11521 cgcggccgcg ctggtccgtc cgcagaaggc cgaggggatg aaggtctcct cggtgaggaa
    11581 gaagctgaag gagaaggcgt tcgcgcccgg cgtgaaccgc gaggagatca cctcggtcga
    11641 ggagagcatc ggcctcccgc tggaagactt catcgccgcc gggatcgagg ggctccagga
    11701 ggtggccccc cagatcgagc tcagcgggtg agctcgtcct cctccgccgg ctccggctcg
    11761 tcgcgctcct gctcctgtct ccgggccgcg gcccggcgcc acagccacca cccgatcaga
    11821 gccgccgtcg ccacgcccac gccgatcacc accggccgcg agtggcgcag gaacacctcc
    11881 cagttctcgc ccagcagcac tccggcccac gccagcgcga acgaccacgg aaggcatccc
    11941 aggaacgtgt agagcgtgaa ccgccaaaac ggcatccggg ccacgccggc cgggaagctg
    12001 atgaacgtcc ggatcacggg tagcaggcgc gtgacgaaga ccgtgagctc cccgcggctc
    12061 gcgaaccact cgtccgctcg atcgagctcc ttctcccgga ggaagacgta gcggccccag
    12121 cgttcggcca gcggccgccc cccgacccgc cccacgtagt aggccagcca cgaccccgcc
    12181 acgttcgcga ccgcgccgat cgtccccacc gctatgaagt cgaggtcccc tcgggccacg
    12241 agaaacccgc cgaacaccat cgtgacctcg gaggggatcg ggatcagcgc cgactccaac
    12301 atcatcagga cgaagatggc gaagtagccg tacgtgccga tcacgtcgac gatcaggtgt
    12361 tcgagccact ccagcatggg aatggccagc ctagcagttg cgttatctgg acggttcgac
    12421 gcgctatcct cgcgcgtggc gtctcgttga cgccgatcct tccctcgtgt atgaaagcgg
    12481 gctcgtggca aacaaactcg tcatcgtcga atcgcccacc aaggccaaga ccctggagcg
    12541 gtacctcggc ccgggctacc gggtcaaggc ctcctacggc cacgtccgcg acctccccaa
    12601 gagcaagatg gccgtcgaca ccgagcggaa cttcgagccg gagtacgagg tgcccgacgc
    12661 caagaagaag gtggtcgcgg agctgaagcg tgcccacaag caggccgacg agctgtggct
    12721 ggccaccgac ttcgaccgcg agggcgaggc gatcgccttc cacgtggccg agctgctcgg
    12781 ggtgagcccc gaggaggcga agcgcgtcac cttcaccgag atcacccagt ccgcggtgcg
    12841 ggaggccttc gccgagccgc ggacgatcga cctccaactc gtcgaggccc agcaggcgcg
    12901 tcgcatcctc gaccggctcg tgggctactc gatctccccg ctcctgtgga agaaggtgcg
    12961 gccgggcctg tcggcgggca gggtccagtc gccggcgctg cggctcatcg tggagcgcga
    13021 gcgcgagatc gccgccttcc agcccaagga gtactggagc gtggacgcgc gcctcgcgcc
    13081 gaacggcgac gaggacgcgt tcctggccgg cctgtggcag atcggcgagg agaagctcgc
    13141 cgccaaccca gacaagaagg ggctggtgct cgagagcgag gaggtggccc gggagcacgc
    13201 gggcaacctc gagcgcgcta cctaccgggt gaccgaggtc cggcgctccg agcggaagcg
    13261 gaacccctcg cccccgttca ccacctcgac cctccagcag gaggccgcgc ggaagctcgg
    13321 gttcacctcg aggaagacga tgcggatggc ccagcagctc tacgagggcg tggagctccc
    13381 cggcgagggt caggtcgggc tcatcaccta catgcggacc gactcggtga cgatcgccga
    13441 gcaggcgctg gccgagatcg ccgacgtggt ccgtctccag tacggcaagg agtacgcgct
    13501 ggacaagccc cgccgctaca agaagaagca gcgcggggcg caggaggcgc acgaggcgat
    13561 ccgacccacc tccgccgccc gtctccccga cgtcgtccag gcccacctcg accgggacca
    13621 ggcccgcctc taccggctga tctggcagcg aacgatggcc agccagatgg ccccggccgt
    13681 cttcgaccag gtgtcggtgg acatcgaggc ggcggccgac gatgcgccgc gctacctcct
    13741 ccgcggcacc gggcagacga tgaagttcga cggcttccgc cgcgcctact tcgagggccg
    13801 ggacgacgac gtggacgagg acgcggagtc gcgcctcccc gagctcaccg ccgagcaggc
    13861 cctccgcctc ctggaggttc tccccgagca gcacttcacc cagcccccgc cccgctacac
    13921 ggaggcttcg ctgatcaaga aactcgagga gctgggcatc ggccgcccgt ccacgtactc
    13981 ctcgatcgtc tccaccctgc tcgatcgcga gtacgtgcgg ctcgaggagc gccgcttcta
    14041 tcccgaggac gtgggcgagg tgacgaccga cgtgctcgtg gaccggttcg gggatcactt
    14101 cctcgactac gagttcaccg ccaggatgga gggcgagctc gacgacatcg ccgagggcaa
    14161 gatgcgctgg aaccaggtgc tcgacgagtt ccacgccccg ttcgagcgga aggtggagaa
    14221 ggccgaggag gagctggaga ggccggagga ggagatcgac gagctctgcc cgcgctgccc
    14281 cgaggagggg cgagagcccg ggaagctcgt gatcaagctg gggcggcggg gcaagttcgt
    14341 gggctgcaag aactacccgg agtgccgcta caccagggac gtggacggcc aggagcgacc
    14401 cgagcccgag ctgctggagg agacctgccc cgagtgcggc aagcagctcg tgaagcgctg
    14461 gggtcgctac gggccgttca tcggctgctc cggctacccg gactgccgct acatcaagcg
    14521 ggaggagaag aagaccggcg tgacgtgccc caagtgcggc gagggcgagc tgatcgagaa
    14581 gcgggcccgc cggcgccgcg gcaacgtctt ctacggatgc aaccgctacc cggactgcga
    14641 cttcacggtc ggccagcggc cgctgaccga gccctgcccc gagtgcggcg cgctgatgac
    14701 cgcgcagaag gacggcagcg ggaagtgcca gaacgggcac gtgatcgagg gggccgccga
    14761 gcgctcggcc cccgcgagcg aggagaagga gccggaggcc accggctccg ggtcggcgga
    14821 agcgggctag gttggtcgac tacgccgacg agatcgcggc gcttcggggc acgaagccag
    14881 cgaccttcgg cagcctcttc aagaaccgat cgttcttccg cctgtggcgg gcgatgctcg
    14941 tctcctcgct gggggactgg acgggcttcg tcgccatcac ggcgctggta acccggatcg
    15001 ggggcccccg cgccggctac gcggtcaccg gcgtgatgct ggcccgcctg ctcccgtcct
    15061 tgatcttcgg gcccgtggcg ggcgtcttcg tcgaccgctt cgaccgcagg aagctgatgg
    15121 tgatcgcgga catcggccgg ggggccgggt acgtctcgat ggcgttcgtc ggcgagctgt
    15181 ggctgatcta cgcgctctcg ttctcagtgg agtgcctctc gctgctgtgg accccggccc
    15241 gggacgccag catccccaac atcgtgtccc ggcggcagct ctccaacgcc aactcgatgg
    15301 ggttgttcac ctcgtacggc acgcttcccc tcggagggat cctcttcacc gcgctcaccg
    15361 cgttggcgac cggcatcgga ggcgtgatcg agtacttcgg ggaccggccc gagtcgctcg
    15421 ccctcctgct caacagcgtc acgtttgcct tctccgcgtg gatgatctgg ggcctccagc
    15481 tcccggagcg gagccgtcgg gcccagggca ggctgcgccc ggcgcaggcc tgggacgacc
    15541 tgcgggaggg gatccggttc ctccgcgccc acgccttcca gcggacgatg acgatcggcg
    15601 tggtgctggc cttcaccggc gtggggtcgg tgatcgcgct cggcccgatc ttcgcgcaga
    15661 acacgctcgg ggcgggccag gcgggctggg ggatcgtcgt gacgtcggtg gggctcggcc
    15721 tggcggcggg gatgatctcg gtcgggctgg tcgagcgcgt gatcgacaag gagaccctct
    15781 tcccgctggc gatgatcgga gcggccgggc tgctggtcgt gctgtcgctg atgcccaata
    15841 tcgcgctggc cgcgatgctg acggcggtga tggggtcggt ggccgggttg gcgtgggtcg
    15901 cgggctacac cctcctccac gagaatgtgg gcgatgagtt ccgaggccgg accttcgcca
    15961 cgctcacgat cctgtcgagg ctcggccttc tcgcctcgct ggcgggcttc cccgcgctgg
    16021 cgcagaccat cggcgactac tcgatcggga tcggcgacgc cgagatcgat ctggccggga
    16081 cgcgtctggc gctgtgggtc ggagcggccg tcgtcctggt gggcggcgtg agcgcacgga
    16141 gggggctccg ccggggacgg cgctccaggg cccagcccct ccagctccgc cctcagctcc
    16201 gtcgcgggga gcggccgggg accttcattg tcttcgaggg ggtggaggga tccggcaagg
    16261 gcacgcaggt cgagctggcg aaggacttcc tcgagggcga ggggtacgag gtcctggtga
    16321 cccgggagcc gggcgggacg ccgctggggg agggcctccg cgaggtgctg ctgggcgacc
    16381 agccgctgga cgcgagggcc gaggccctcc tcttcgccgc cggtcgggcc cagcacgcgg
    16441 tggccgtgat ccggcctgcg ctggagaagg gcaaggtcgt cctgtgcgac cggtacgtgg
    16501 attcgtcgct cgcctatcag ggggtggggc gcgatctggg ggagccggac gtcctggcgc
    16561 tctcggcctg ggccacgcag ggcctcttcc cggatctcgt gatcctgctc aacatcgatc
    16621 ccgaggaggg cctggagcgg tcggggggcg acgatcgctt cgagtccgag ggagacgagt
    16681 tccacgagaa ggtggccgag gcttacctgc ggatcgccga cgagcacccc gagcgggtcg
    16741 tcgtggtgga cgccctgggg acgcccgagg aggtccatgg gcgggtgcgc gacgcgatcc
    16801 gccgcttcct aaaggggcag gaggaggcgt gagcggggtc ccggtcctgg cctccgtccc
    16861 ggggcaggac gaggcgatcg cgttcctgga gcgggcggcg gagcgccccg gccatgccta
    16921 cctgttcgcg ggccccgagg ggtcggggaa gaagctggcc gccgcggctt tcaccgccgc
    16981 cctgctgtgc gagcggggcg ggtgcgggga gtgccgggac tgccgcctgg cgctggagga
    17041 ccaccatccc aacgtggcgg tgctcgagcc ggaggggcgg gacatccgcg tgggcgaggg
    17101 ggcggacgac ttcgggacgg cccggtggct cgtggcccgg gcccaccgga cggcgcccga
    17161 gccgggaagg aaggtgttcc ggatcgagca ggccgaccgg atgaccgagg aggcggccga
    17221 cgtcctgctg aaggtggtcg aggagcctcc gcccgagacg gtgttcctcc tgatgtcggc
    17281 gcggcctcac gagctgaccg agacgatccg ctcgcgatgc cagaccgtgg cattccatcc
    17341 gctggcggag gggttcgtgg tggagacgct cacggcggag ggcgtgagcg aggggcgggc
    17401 gatgctcgcc tcgcgcctgg ccggagggaa cgtgggccgg gctcgaagga tcgcccggga
    17461 cccggagggc ctggcgttcc gcgacgaggc gctggccgcg ttgcgcgagg tcggaggcga
    17521 cccgcggggg gccctggccg gtgcccagcg gctcgtggac gccgcggccg actacaagaa
    17581 ggggctgggc cgcgtgctcg acgaggagct ggagccgctg ctcgacgagg agacgggccg
    17641 ggtggccgag cagttccggg ggctggccaa gcggatcgac gagcgccaca agcggaggga
    17701 gcggagggcg gagcgcgagt tcctcgactg gtcgctgctg gccctcgagg cctggtaccg
    17761 ggacgccctg ctgatctcga ccgggggaga cccctcgtgg gtgatcaaca tggatctcga
    17821 ctggagcgcg gacggggtgt cgtcggcgag cgcggccgcc gccatcgagg cgctcgagga
    17881 ggctcgggcc gcgctcgccg acgacaccaa cctcaacgcg aagctcgtcg tggagcgggc
    17941 cttccttcgg atcgcgagct gaaccctacc tcgggcagct ccggaacgga tcgtcatccg
    18001 tctcgggcca acgctcccgt tcctcgccga tgctgaccgt gtacgtgtct cgctccacct
    18061 gctcgaagaa ggccccatcc gcgcgataga acgtcctcgt gatgtcgagg cgggtgtcat
    18121 cctgcccgcc atcggcgaag gtccccaccg tgatgacgat catcgcggcg cggaggttcg
    18181 gggcagcgca gtccacgcca gcggcgaact cgccccccag cggcacgaag ggagtcggta
    18241 tcgggcccct cggattcctg gagagcgggt cgtaggtctg cattggggcg atctcaccct
    18301 ggtccatcgt gaataccact ccggttcgat gggggtgacc cgggccgccg atgtcgacca
    18361 cgatctcgtg tccgggctct ccgtcgatct cggcggccat gaggatctcc ggcacgcttg
    18421 gcaccccctc catgggcggg accacggcgg agaggggagt cccgacggga ggctccacgt
    18481 agaggaagta gcggcattcc ggaggccgct cctcgtccgc gatgagccgg acccgggcgg
    18541 tctcgccgtc accgtccaca tccgtctccg tctcgtcgat cggcctgtag gccgccagaa
    18601 atggcacacc gtcgttgcca cacggaagaa cgtcgggcgc agatggggag gggctgggtg
    18661 tcggtccgac gaccggtccg ggcaccggct gccacgagat gcccccgacc tcgccgatcc
    18721 ccgagacgat ctctctgggc tcccccccga tcggcgcgac tctaaccgac cccggggtgg
    18781 agtacgccac gagatttccg ttgggagacc atgcgatcgc cgggatctcg ccctcgacat
    18841 cgatcgccat cacggggttc tcgggggcgt tgatgtccag cacggtcacg gtgagcgcgg
    18901 ggacatctcc accgacgaac gcgatccgcg agccgtccgg agaccatgcg ggcaccccgt
    18961 ggcatgcgcg atcgaagagt cttgtcgtca gggcggttcg aggctcgacg atgtggagac
    19021 cgaaggaacc atcctgactc gtgtcgaggt ccacgccgga gcatccctca cgcgcccatc
    19081 ggttgaggaa cgcgatccgg gaaccatccg gcgaccatac gggcgaggtg ggcctttcca
    19141 tcatctccgt gagctgagcc acctcgtggg ccccgagatc ccagaggtag atctgcggct
    19201 gatccccgcc gcccacgaat gcgatccgcc cgccgtctgg cgaccacgac ggtgacccca
    19261 tcgcgcgcac ccgggaggga tggctgatgg tcctcgtgcc gccggacggg aggaacggct
    19321 catgcgtgta caacgaaccg tcggcgtcgg ccatgctgat cgccctgccg tccggtgacc
    19381 acgcgggggc gaacatctcc acgggacttc cgaaggactc acgccgaccg gtcccgtccg
    19441 ctcgcacgat gtggaggttg ccggcacccc cgcgctcggt gacaaaggcg agccttgagc
    19501 catccgggga ccagaccggg tggatctcgt cgtgcggacc aacggccagc ggtctcagcc
    19561 ccgtgccgtc cgggttgacc acgtagatgt cccagccgcc ctcggtctgt acggagaacg
    19621 cgatcagacc gtcgcgaccc cccgggacgg cgggccgact ctcatccaag ttgaaagctc
    19681 gactcagtcc ccagactcct gccgcggagc cgaggaccac ggcgacggcg agcgccccgg
    19741 ctcgcgcgcg ccgcgccgtg cggtatcggc gcttgcgccg gaccacgtcg tcgatcgccc
    19801 ccgagccgtc ggcgcggggc gccagacgct ccagcccctc gcggatgcga tcgtcaagct
    19861 ccgacacggt cgtcctcccg gtcgttcagc gcctctgcga gggccttcct cgcgcggtgc
    19921 agcgtggtct tcaccgttcc ttcgctgacc ctgaggatcc gagccatgcc ggccacgtcc
    19981 tcgcccaggt agtagcgcag caccacgacc tcccgctggc gcctcgacag cccgcgcagg
    20041 gcccgctcca agtcgacgac gtcctcggcc gggcgctcgt cgctccgcac ggccgccaat
    20101 cgcgcccacg ctcggcgctc ggccatcgcc cttcgccatc ggctcctgga aaggttcatc
    20161 gccaccgtcg tcacccaagc ggccagcgtc tcgatgctct cgccccgctc ggtccgctcc
    20221 caggcgcggg ccagcgcctc ctgcacggcg tcctcggccg cgacgcgaga gccaccgacc
    20281 agcgcgatcg ccgccaccac ccgtgggtac tcctccttga ggaacgctct gatctcggtc
    20341 tcgctcagcg ggctgcccct ttcacccgga cgacgcgtga cggcgacgac aggttgacac
    20401 gaggagggtg atccggctca aaaatgagcg cttcgcgccc gatgggtgat ttcgacgacc
    20461 cgggaaggtg aggcgtgagc gagcagatgg ccgaagtggc gggcagggtc ttcgtcgctt
    20521 tcctcttcgt gctcggtggg ctggtcgtgt ggcgctggct caggagcggg tcgccgtggg
    20581 ctcggaggat ctccttggcc ctgtcgatcg tggcgggcgg gatcatgctc gtcgccgcgg
    20641 tccaggctgc tctggggagc ggcgacgaga gccgcgagcc caccgggcag gccgaggtcg
    20701 tcttcctcgg ggttcccggc tacgagctct ggccggacct cgagctcgag agggccgcgg
    20761 agagccagct gcaggatgat ccggcgctgc acgacaagtg ggccgacgtg tcgatccgga
    20821 aggcctaccg agggaacggg ctcgtggccc aggcgatggc gatcgagctg cggccgggcc
    20881 tcggcgacac ggaggggctc ctcgacgacg tggcgagggg gcctgtcgca ggtcggcggt
    20941 gaggagctcg tggagcgtcg gatgcgcggg gtgtcgatcc gggtctcgcc ccggttccgg
    21001 ggcgctcggg gctccgtgta cgtgatgacg tggctcagag gggacgtcat cgtcaccgtc
    21061 tcggggcagc gggcgggcgc cgtccggcgg ttcgcggccg cggtgatcga ggccgaccga
    21121 gaggcctgag ccggttgcta caatctcggt gaccgcctcc gccggagtag ctcagttcgg
    21181 tcagagcaca cgattcgtaa tcgtggggtc atgggttcga atcccatctc cggctcttcc
    21241 ggtctcgatt gcctttcttc ggagagcctg gttagactcg acccacaccc cggtcgagga
    21301 ggcgctcatc ctcaggacca cggtgacgat cgcgacgctc gcggccatcg cgatagggct
    21361 gccggcgccc gggagcgcca cgcagggacc gcccgagccc atgcccggtc tgatcgacgc
    21421 ggccgagatc gcccgcgacg agcgcgggat cgctcacatc accgcccgga acgaccacga
    21481 tctcttcttc ctccagggct gggtccacgc gggggatcgg ctgttccaga tggacgtgag
    21541 ccggcgccag
//
LOCUS       DASUTZ010000117         4528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1067308, whole genome shotgun sequence.
ACCESSION   DASUTZ010000117 DASUTZ010000000
VERSION     DASUTZ010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067308"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..339
                     /locus_tag="VF097_05345"
     CDS             94..339
                     /locus_tag="VF097_05345"
                     /inference="COORDINATES: protein motif:HMM:NF039800.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HEX6262255.1"
                     /translation="MSKRYDEQVSVEQDGGLPAAFLWRGRRHEVSDVIGRWRIEGGWW
                     EDGRDREYWRVEARGGAVFDLFLDRREGSWRLERLWD"
     gene            complement(340..2028)
                     /locus_tag="VF097_05350"
     CDS             complement(340..2028)
                     /locus_tag="VF097_05350"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6262256.1"
                     /translation="MSPARALLVALSLVAAACSAGAPAARRSPEPGGGSPSVAPTAST
                     RMEEVACGLPAPWILRIARGYHPERSAELQILPREPNFVGAGLPHVGPWTYTSHVPMF
                     WYGPGHIEPSGRVRRAATLADIAPTQARMLGLDFPTPDGKPLTEALVQDPSGPPKLIV
                     TVIWDGAGRHVLDEWRGRWPNLRRLLPEGAWYEEAFAGSSPTSSAQGHGIIGTGTFPR
                     THGLVGHSIRIGDRIVPPWRTGPDLLLRPTLADLWMQETGGRAKAGLLGTVGIQLGML
                     GHGSAWEGNPKPLVVLREAPDAETLGAEGTRWNLPEHLLEFYRFPGYANDLPELAGYF
                     PDMDRRDGQADGTWRGHALDDEVIQGGFQTPARIPYQTRLLEEVIERERFGEDDVTDL
                     LFVNYKLIDQLGHIFSMNSEEMGDAVEVQDEHLPTLIDLLDRLVGRGEWAMVITADHG
                     STPSPEVSGAFQISSTELHQRLNGAFDRDDDDRPVVQQVKQTEIFVDTEELAEHGGTL
                     EEMAEYVMDLRQEDTAIPDVSNVTDPSARVFQAAFPAEIIPGLPCLPEDIGASG"
     gene            2092..2442
                     /locus_tag="VF097_05355"
     CDS             2092..2442
                     /locus_tag="VF097_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262257.1"
                     /translation="MTERARDALRRELYWGDEPRAAGPTRELRARIERLADSLQADLA
                     RPFPADPSFSGGSRWRLGVKRFLYRLNRPLTRRQDRIAAELAAISLELLDHLGRLEDE
                     LRRVEERSPPGDAS"
     gene            2439..3488
                     /locus_tag="VF097_05360"
     CDS             2439..3488
                     /locus_tag="VF097_05360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6262258.1"
                     /translation="MRIAVCHPQTPFTRGGAEMHMEGMVRALRADGHDVDVVTMPFKW
                     YPPSEIVHQMASWRSVDLSEANGQRIDMVIALKFPAYLVAHERKVVWLIHQHRTAYEL
                     WDHPEYGDLRLHEDGEAVRDMIHRADRVGLSEAKRIFTNSANVRDRLRRSVGLSADVL
                     YHRSPLCEALLEIEPGPHGDHVLFPSRLEPLKRQSLAIDAMRHVRSGARLVLVGKGPG
                     EDALRRRIAEAGLEDRVELAGPVPDEDLIELYRTALAVFYGPYDEDYGYVTIEAMAAG
                     RPLVTCTDSGGPLELVRDGESGRVVASEPRAIARAIDALHEGRDEAARMGAAGRSLVE
                     ARVPPWPEVARRLLS"
     gene            3485..>4528
                     /locus_tag="VF097_05365"
     CDS             3485..>4528
                     /locus_tag="VF097_05365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6262259.1"
                     /translation="MRVAFLTPLPPAPTGIAAYSRAVLDRLERRGVGHELVPRAPRSL
                     EEAERLAETSDLAVYHVGNNPLFHRHIYGAAIRRPGLVVLHDLALDDLIRGLDAASDP
                     AAGPARTEARAAARALRTSGLDVDPPLATPWSAHLVRRARGVIVHAPFGARYLEAFGC
                     RTPVFVVPHPPVPEPSGSRAARAERRVRRRLAGRSPVVGVLGDIGGAKGIEAVLDAVA
                     RLEQPALLAIVGRRIPGFDAAEAVGRSGLGDRVVVEQDVSDVVFGAWLRACDVVVNLR
                     HPHRGEVSGTLVRAMQAGVPTVVSATGTYLDLPDDAVVRVPAGPPDPQALVEALGVLL
                     ADPARRRAIGERAR"
BASE COUNT          565 a         1635 c         1694 g          634 t
ORIGIN      
        1 cccccccccc cccccccgac cccgcttgac agctcgccgc gaccggcggt acggtttccc
       61 tctctcgaac atacgttcga acgggaggag gccgtgtcca agcggtacga cgagcaggtc
      121 agcgtggagc aggacggcgg cctcccggcg gccttcctct ggcgcgggcg gcgtcacgag
      181 gtctccgacg tgatcggccg gtggcggatc gaggggggct ggtgggagga cggccgcgac
      241 cgggagtact ggcgcgtgga ggcccggggc ggggcggtct tcgacctctt cctcgatcgc
      301 cgcgagggct cctggcgcct ggagcgcctc tgggactgac tacccgctgg ccccgatgtc
      361 ctcggggagg cagggcagcc ccgggatgat ctccgcgggg aaggccgcct ggaacacgcg
      421 cgccgagggg tcggtgacgt tcgagacgtc ggggatcgcc gtgtcctcct gccgcaggtc
      481 catcacgtac tcagccatct cctcgagcgt ccccccgtgc tccgccagct cctcggtgtc
      541 cacgaagatc tcggtctgct tcacctgctg gaccacgggg cggtcgtcgt cgtcccggtc
      601 gaaggccccg ttgaggcgct ggtggagctc ggtggaagag atctggaacg ccccgctcac
      661 ctccgggctc ggggtcgagc cgtggtccgc ggtgatcacc atggcccact cgccccggcc
      721 caccaggcgg tccaggaggt cgatcagtgt gggcaggtgc tcgtcctgga cctcgaccgc
      781 gtcccccatc tcctcgctgt tcatcgaaaa gatgtggccc agctggtcga tcagcttgta
      841 gttcacgaag agcaggtcgg tgacgtcgtc ctccccgaac cgttcgcgct cgatgacctc
      901 ctcgagcagg cgggtctggt aggggatcct cgcgggcgtc tggaagccgc cctggatcac
      961 ctcgtcgtcc agggcgtggc cccgccaggt gccgtcggcc tgcccgtcgc gccggtccat
     1021 gtcggggaag tagccggcca gctccgggag gtcgttcgcg tacccgggga agcgatagaa
     1081 ctcgaggagg tgctccggca ggttccaacg cgtgccctcc gcccccagcg tctccgcgtc
     1141 cggcgcctcg cggaggacca cgagcggctt cgggttgccc tcccaggccg agccgtgccc
     1201 gagcatcccg agctggatgc cgaccgtgcc cagcaggccg gccttggccc gcccccccgt
     1261 ctcctgcatc cagaggtcgg cgagcgtggg tcggagcagg aggtcggggc cggtgcgcca
     1321 cggcgggacg atgcggtccc cgatccggat gctgtggccg acgaggccgt gggtccgggg
     1381 gaacgtcccg gtcccgatga tgccgtggcc ctgggccgag ctggtggggg acgagcccgc
     1441 gaacgcctcc tcgtaccagg cgccctcggg gagcaggcgc cggaggttcg gccagcgacc
     1501 ccgccactcg tccaggacgt gacgccccgc gccgtcccag atcacggtga cgatcagctt
     1561 gggaggcccg ctcgggtctt ggaccagggc ctcggtgagg ggcttgccgt ccggcgtggg
     1621 gaagtcgaga cccagcatgc gggcctgcgt gggcgcgatg tcggcgaggg tggccgcacg
     1681 ccggacgcgg cccgacggct cgatgtggcc ggggccgtac cagaacatcg gcacgtgcga
     1741 ggtgtacgtc cacggcccga cgtgggggag gcccgcgccc acgaagttcg gttcccgggg
     1801 gaggatctgc agctccgcgc tccgctcggg gtggtagccg cgggcgatcc gcaggatcca
     1861 cggcgccggg aggccgcacg ccacctcctc catccgcgtg gaggcggtgg gagcgacgga
     1921 aggcgacccg ccgcccggct cggggctgcg cctcgccgcc ggcgcgcccg cggagcacgc
     1981 ggcggcgacc agcgacagcg cgacgaggag cgctcgtgcc ggagacatac ggacgacagg
     2041 gaacaccatc ttgggcagaa cggcaatgat gctactggta aggtgcctcc gatgaccgag
     2101 cgagccaggg acgccctgcg tcgagagctc tactgggggg acgagccgcg ggccgccggc
     2161 cccacgcggg agctccgcgc ccggatcgag aggctcgccg actcgctcca ggcggacctg
     2221 gcccggccgt tcccggcgga cccgtccttc tccggcggct cccggtggcg gctgggggtg
     2281 aagcggttcc tctacaggct caaccgtccc ctcacgcgtc gccaggatcg gatcgcggcc
     2341 gagctcgccg cgatctccct cgagctcctc gaccacctgg ggcgccttga agacgagctc
     2401 cgccgggtcg aggagcgatc gccgcccgga gacgcgtcgt gaggatcgcg gtctgccacc
     2461 cgcagacccc gttcacccgc gggggcgccg agatgcacat ggaggggatg gtccgagcgc
     2521 tgcgggccga cggtcacgac gtcgacgtcg tgacgatgcc cttcaagtgg tacccgccgt
     2581 ccgagatcgt ccaccagatg gcgtcgtggc ggagcgtgga cctctccgag gcgaacggcc
     2641 agcggatcga catggtgatc gccctgaagt tcccggccta cctcgtggcc cacgagcgga
     2701 aggtggtctg gctgatccac cagcaccgga cggcctacga gctgtgggac caccccgagt
     2761 acggggacct ccgcctccac gaggacggcg aggccgttcg ggacatgatc caccgcgccg
     2821 accgcgtggg cttgtcggag gcgaagcgga tcttcacgaa ctcggcgaac gtccgggacc
     2881 ggctgcggcg gtcggtgggc ctgtcggccg acgtcctcta ccaccggtcg ccgctgtgcg
     2941 aggcgctgct cgagatcgag ccgggcccgc acggcgacca cgtgctgttc cccagccggc
     3001 tcgagcctct gaagcgtcag tcgctggcga tcgacgccat gcggcacgtg cggtcggggg
     3061 cgcggctcgt cctcgtgggg aagggtcccg gcgaggacgc gctacgccgg cggatcgcgg
     3121 aggcggggct cgaggatcgg gttgagctcg ccgggcccgt cccggacgag gacctgatcg
     3181 agctctaccg cacggcgctg gccgtcttct acggcccgta cgacgaggac tacggctacg
     3241 tgacgatcga ggcgatggcg gccggccgcc ccctggtgac ctgcacggac agcggcggtc
     3301 cgctggagct cgtccgcgac ggagagagcg gccgcgtcgt ggcgtccgag cccagggcga
     3361 tcgcgagggc gatcgacgcc ctgcacgagg gccgcgacga ggccgctcgc atgggggcgg
     3421 ccggccgctc cctggtcgag gcccgggtcc ccccgtggcc cgaggtggcc cgccggctcc
     3481 tgtcgtgagg gtcgcgttcc tgacgccgct gccgcccgcg ccgaccggga tcgccgcgta
     3541 ctcgcgggcc gtgctggatc ggctcgagcg ccgcggggtc ggccacgagc tggtgccgcg
     3601 cgcgccgcgc tccctcgagg aggccgagcg gctggctgag acgagcgatc tcgccgtcta
     3661 ccacgtgggg aacaaccctc tgttccaccg gcacatctac ggggcggcga tccgtcgtcc
     3721 ggggctcgtg gtgctccacg atctcgccct cgacgacctc atccgggggc tcgacgccgc
     3781 gtcggacccc gcggcggggc cggcccggac cgaagcccgc gcggcggcgc gggccctgcg
     3841 gacgagcggt ctcgacgtgg acccgccgct cgccacgccc tggtccgccc acctcgtccg
     3901 cagggcccgc ggggtgatcg tccacgcgcc cttcggcgcg cggtacctcg aggccttcgg
     3961 ctgccgcacg cccgtcttcg tcgtcccgca tcctccggtc cccgagccgt cgggctctcg
     4021 cgccgcgcgg gcagagcgcc gcgtgcgccg ccgtctcgcc ggccggagcc ccgtggtggg
     4081 cgttctcggg gacatcgggg gtgccaaggg gatcgaggcc gtgctcgacg ccgtggcccg
     4141 gctcgagcag ccggccctcc tggcgatcgt ggggcgccgg atccccggct tcgatgccgc
     4201 ggaggccgtg ggccgcagcg ggctcgggga ccgcgtcgtc gtcgagcagg acgtctcgga
     4261 tgtcgtgttc ggcgcgtggc tccgagcctg cgacgtcgtc gtgaacctcc gccatccgca
     4321 ccgcggggag gtgagtggga ccctcgtgcg cgcgatgcag gcgggcgtcc cgacggtcgt
     4381 cagcgccacc ggcacgtacc tcgacctgcc ggatgatgcc gtggtgcggg tgccggccgg
     4441 cccgcccgat ccccaggccc tggtcgaggc gctcggcgtg ctcctcgccg accccgcgcg
     4501 ccgccgagcg atcggggagc gggctcgg
//
LOCUS       DASUTZ010000118         2100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1067410, whole genome shotgun sequence.
ACCESSION   DASUTZ010000118 DASUTZ010000000
VERSION     DASUTZ010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067410"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="VF097_05370"
     CDS             <1..1003
                     /locus_tag="VF097_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX6262260.1"
                     /translation="IASFIGAPGPETIVFNRGTTESVNLVAHAWGRRFLKEGDEILIS
                     ELEHHSNIVPWQLAARDTGALLKYIPVTDEGRVELEAVEGAITDRTKLVAITGQSNVL
                     GVMPAIADIARLAHDAGARFLVDGAQLVPHAPVDVQAIGCDFLTISGHKMLGPTASGG
                     LYATTEVMEEMDVFLGGGEMIEEVLKEEATYKPPPYKFEAGTPNIAQEVGLGAAVDYL
                     QGIGMEAVHEHEIELTAYALERLGELGGVRVFGPTDATERGGAISFWVEGSHPHDVAT
                     ILDEEGVCVRAGHHCAQVLMRRLGVPATARASFNVYTTRDDVDRLVEGLEKVRAVFG"
     gene            1000..1443
                     /locus_tag="VF097_05375"
     CDS             1000..1443
                     /locus_tag="VF097_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003352484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEX6262261.1"
                     /translation="MIDELYKEVILDHYKSPRNKRGIEAATCSSHGNNPLCGDEVTVY
                     VNVEDGQVTEAAFEGQGCSISQASASMMTEAVTGRSLDEVHSLTKEFRSMMAGEAEPS
                     EETLGDLAALTGVVRYPARIKCAVLAWDTLEQALEGGEAHGAAAH"
     gene            1451..2074
                     /locus_tag="VF097_05380"
     CDS             1451..2074
                     /locus_tag="VF097_05380"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6262262.1"
                     /translation="MATLRRAVSEDAHAVAPLILEAAPHLRVLLGGDHEAWHAAEHAF
                     HSERTMYGWKWGMVAEEDGEAAGFVIAFPGRRWASLKLGTNVVLTREAGVKHAAELVR
                     RRRVLDRLHPPVPEWTLYVSALAIHPGHRRKGIATTLMDRVIAGADNLDLAVAVDLDL
                     DADAAWELYDSLGFVQAESREASESERALLQTVGFARLVRQAGPRAS"
BASE COUNT          330 a          688 c          804 g          278 t
ORIGIN      
        1 gatcgcttcg ttcatcgggg cgcccggccc cgagacgatc gtcttcaacc gcggcacgac
       61 cgagtcggtg aacctcgtgg cccacgcctg gggccgccgg ttcctgaagg agggcgacga
      121 gatcctgatc tccgagctcg agcaccactc gaacatcgtg ccgtggcagc tcgccgcccg
      181 ggacacgggc gccctcctga agtacatccc cgtcacggac gaggggcggg tggagctcga
      241 ggccgtggag ggcgcgatca ccgaccggac gaagctcgtg gcgatcacgg ggcagtcgaa
      301 cgtgctgggg gtgatgcccg cgatcgcgga catcgcccgg ctcgcccacg acgcgggggc
      361 gcggttcctc gtggacggag cgcagctcgt gccgcacgcc ccggtggacg tgcaggcgat
      421 cggctgcgac ttcctcacga tctcgggcca caagatgctc ggccccaccg ccagcggcgg
      481 gctctacgcc acgaccgagg tcatggagga gatggacgtc ttcctcggcg ggggggagat
      541 gatcgaggag gtcctgaagg aggaggccac ctacaagccg ccgccctaca agttcgaggc
      601 cggcacgccc aacatcgccc aggaggtcgg gctgggcgca gccgtggact acctccaggg
      661 catcgggatg gaggctgtcc acgagcacga gatcgagctc acggcctacg cgctggagcg
      721 gctcggggag ctcgggggcg tccgggtctt cggaccgacc gacgccaccg agcggggcgg
      781 ggcgatctcg ttctgggtgg agggctcgca cccgcacgac gtggccacga tcctcgacga
      841 agagggggtc tgcgtccggg ccggccatca ctgcgcgcag gtcctgatgc gccgcctcgg
      901 cgtcccggcc accgcgcgcg cgagcttcaa cgtctacacg acccgggacg acgtggatcg
      961 gctcgtggag ggcctggaga aggtcagggc ggtgttcgga tgatcgacga gctctacaag
     1021 gaggtcatcc tcgaccacta caagtcgccg cggaacaagc gggggatcga ggcggcgacg
     1081 tgctcgtcgc acggcaacaa cccgctctgc ggcgacgagg tcaccgtgta cgtcaacgtc
     1141 gaggacggcc aggtgaccga ggccgcgttc gaggggcagg gctgctcgat ctcgcaggcc
     1201 tcggcctcga tgatgacgga ggcggtcacc gggcgatccc tggacgaggt ccactccctg
     1261 acgaaggagt tccggtcgat gatggcgggg gaggccgagc cgtccgagga gacgctgggt
     1321 gacctggccg cgctgaccgg ggtcgtccgc taccccgccc gcatcaagtg cgccgtcctg
     1381 gcctgggaca ccctcgagca ggcgttggag ggcggcgagg cgcacggggc cgccgcgcac
     1441 tgagcggacg atggccaccc tccggcgtgc cgtctctgag gacgcccacg ccgtcgcacc
     1501 cctgatcctc gaggcggcgc cccacctgcg cgtcctgctg ggcggcgacc acgaggcctg
     1561 gcacgccgcc gagcacgcct tccacagcga gcggacgatg tacgggtgga agtgggggat
     1621 ggtcgccgag gaggacggcg aggcggcggg cttcgtgatc gccttcccgg gcaggcgatg
     1681 ggcctcgctg aagctgggga cgaacgtggt gctcacgcgg gaggcagggg tgaagcacgc
     1741 cgccgagctc gtcaggagga gacgggtgct cgaccggctg cacccgcccg tgcccgaatg
     1801 gacgctgtac gtcagcgcgc tcgccatcca ccccggccac cgcaggaagg gcatcgccac
     1861 gacgctgatg gatcgcgtga tcgcgggggc cgacaatctg gatctggccg tggcggtgga
     1921 cctcgacctc gatgcagacg ccgcctggga gctctacgac tccctgggat tcgtgcaggc
     1981 cgagtcgagg gaggcgagcg agtcggagcg ggctctcctc cagaccgtgg ggttcgccag
     2041 gctggtccgt caggcaggac ccagagcgag ctgaggggcg gggggggggg gggggggggg
//
LOCUS       DASUTZ010000119         5909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1846346, whole genome shotgun sequence.
ACCESSION   DASUTZ010000119 DASUTZ010000000
VERSION     DASUTZ010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1846346"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /locus_tag="VF097_05385"
     CDS             complement(<1..1095)
                     /locus_tag="VF097_05385"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6262263.1"
                     /translation="MLLELHIAGLGVIDDATLDLAPGLNVLTGETGAGKTMVTVGLSL
                     ALGVRARTGMVREGAERAGVEARFEASGLAPASEWSDDGELVLARSLSADGRSSARAG
                     GQLAQLSTLAELGADLVEVHGQHQTQRLLSPAAQLAFLDRFCGPEHLERLRGYRAAFR
                     RLRKVRERLAHLDEAAREREREKDLLEYQVREIESAGVRLGERSELELEERRLGHAER
                     ILELMGAARAALADEEGGADELDAAAASIGEAAALDPTAGDLSQRAAALAVEARDLGG
                     ALRAYAEGLELDPARLQEVRERLAALRELERKYGDGEEAVLAYLEEARAKLAAAVGTD
                     EERAELSAEGERLEAEVTGAAAVLRSRRTAA"
     gene            1262..1657
                     /locus_tag="VF097_05390"
     CDS             1262..1657
                     /locus_tag="VF097_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HEX6262264.1"
                     /translation="MTLNQGFGYVFGAIYALVGLLGFTVTGGVEFAGNQGEQLIGFEL
                     NPLHNIVHIAIGALLIAGAAGGLRASRMTNGSVGAAYLLVGILGIFMVNEDWDFLALN
                     QADNVLHFATAVIALAIALWADKREPARA"
     gene            complement(1702..2538)
                     /locus_tag="VF097_05395"
     CDS             complement(1702..2538)
                     /locus_tag="VF097_05395"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX6262265.1"
                     /translation="MRAGLVVHPARPRALEVGAEIVTACRERGIATRSLDEVDVGANE
                     RAAGSLADGADLVVSVGGDGTLLRAALLASEAGAPVLGVNVGRLGFLSDIEPPEVPAM
                     LDAFVAGDLVVEERLTVEAEAEGAPWSDRPWALNEIMVEKRARQRVISLAVHVGGEYV
                     TTISGDGVIVATPTGSTAYSFAARGPIVHPRVECLILTPVSPHMVFDRPLVLAPDEEV
                     QIEVKGDEPGLLAPDGREALELPLGARVRIRRSDRPARLLRHAGAESFFARLARKFSL
                     PS"
     gene            complement(2535..3299)
                     /locus_tag="VF097_05400"
     CDS             complement(2535..3299)
                     /locus_tag="VF097_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017559650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX6262266.1"
                     /translation="MELVRRGLASSRAEAREAIEAGRVLVGGAPAVKVSSLVAPEDPL
                     AVEGEGRAYVSRGGGKLAAALDSFGIDPRGRPVLDAGASTGGFTDVLLQRGAAHVTAV
                     DVGYGQLAWTIRNDPRVRVLERFNVRDLRPEHLEGPPQLLVADLSFISLTKALPALVG
                     VAAEGADLVLLVKPQFEAGRADVGSGGVVRDPAVWARVIREVAAAASEAGAGPLGVVA
                     SPLRGPAGNVEFLLHARVGGEGAELDVERAVAEVPS"
     gene            complement(3314..3667)
                     /locus_tag="VF097_05405"
     CDS             complement(3314..3667)
                     /locus_tag="VF097_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262267.1"
                     /translation="MRDVVRRYVEAGLGTLKPERAEELARELVDWSKRNTERVVAIVQ
                     REVKRQLRIAGAATQDELRELRKRVRTLERRLDKAEKPSRSKSSSKSSASKSSTRKQA
                     STRKTSSARRSRPKA"
     gene            3770..4093
                     /locus_tag="VF097_05410"
     CDS             3770..4093
                     /locus_tag="VF097_05410"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX6262268.1"
                     /translation="MERRLEELLGRLRETNGAHVGASLPEPRVLVLEVSDQEASFWTE
                     LGDGRVGELRSGHPDEAHIRIRAGSDDIVALADGELAFFPAYLSGRVRIEGGALEMLQ
                     LRRLL"
     gene            4093..5139
                     /locus_tag="VF097_05415"
     CDS             4093..5139
                     /locus_tag="VF097_05415"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6262269.1"
                     /translation="MPGRRFIGRSLRSLGGMLALGRLATRLDDNGPDPAEGDHLGAIR
                     GEVRTVRGPRASSLYTEWFPPAEEGGPTIIFTHGLCLTEAVWHYQKRDLCGGAYGAVT
                     WDLPGHGHSDRIAPGELTQEMGIDTLARVVEAYAEPNGVVLVGHSLGGTLTLGYLARH
                     AEAARERVLGAVIVSTPLVQFAHSVAGRWPGAGLEARVLGKAVRYLIESDLVDRRLAT
                     DVGTDLDTLSYRVVRVGFGKHASPTQVRYVRDVIASVPPQVRADAYRAMTAYDVRAHL
                     GRVEHPILVVIGEEDRLVDPEESRTLATSLPGAEVLGLEGVGHAPFLEAPETFNDAVL
                     AFARRLSRPEADTA"
     gene            5136..>5909
                     /locus_tag="VF097_05420"
     CDS             5136..>5909
                     /locus_tag="VF097_05420"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX6262270.1"
                     /translation="MIPIRDANPATRRPYVTWAFLALNVALFLLWQPRGSCPGDLRFT
                     TQAEEEACALELAFTFCHGAIPEELTSFRPMTELPATTQDARFASQLQELRCPEKGVL
                     GSLFSSMFLHGGLLHLGGNMLYLWIFGNNVEDRMGHLVFPLFYLAGGVVATYAHVATA
                     PSSAIPLVGASGAIAAVLGAYLVIWPHARVTTLVIFFFITVIELPAVVVLGLWFLLQA
                     FQGVGSLGVDVGGVAWWAHIGGFAFGAIVAWLFFRGRREP"
BASE COUNT          784 a         2295 c         2010 g          820 t
ORIGIN      
        1 ggccgcggtc ctccgcgagc ggaggaccgc ggccgccccc gtcacctcgg cctccagcct
       61 ctccccctcc gccgacagct cggctcgctc ctcgtcggtc cccaccgctg cggccagctt
      121 ggcccgggcc tcttcgagat aggccaggac cgcctcctcg ccgtcgccat acttccgctc
      181 cagttcccgc agcgcggcca ggcgctcccg gacctcctgg agccgagccg ggtcgagctc
      241 gaggccctcc gcgtaggccc tcagcgcgcc gccgaggtca cgcgcctcca ccgccagcgc
      301 cgccgcgcgc tgcgagaggt cgcccgcggt cgggtccagc gccgcggcct ccccgatcga
      361 ggccgccgcg gcgtccagct cgtcggcgcc cccttcctcg tccgccagcg cggcccgagc
      421 cgctcccatg agctcgagga tgcgctcggc gtgccccagg cgtcgctcct ccagctcgag
      481 ctcggagcgc tcacccagcc ggacgcccgc ggactcgatc tcgcgcacct ggtactcgag
      541 caagtccttc tcccgctccc gctcccgggc cgcctcgtcg agatgggcca gccgctcgcg
      601 gaccttccgg agacgccgga aggcggcccg atagcccctc agcctctcca ggtgttccgg
      661 cccgcagaac cggtccagga acgcgagctg agcggcaggc gagaggaggc gctgggtctg
      721 gtgctgaccg tggacctcga cgaggtcggc ccccaactcg gccagcgtgg agagctgcgc
      781 cagctggccc ccggccctcg ccgaggaccg gccgtcggcc gagagcgagc gggccagcac
      841 gagctcgccg tcgtccgacc actcggaggc cggagcaagg cccgaagcct cgaaccgcgc
      901 ctccaccccg gcccgctcgg cgccctcccg gaccatcccg gtccgggccc gcacgccgag
      961 ggcgagcgag agccctaccg tgaccatcgt cttgcccgcg cccgtctccc ccgtgaggac
     1021 gttgagcccc ggggcgaggt cgagggtggc gtcgtcgatc acccccagcc cggcgatgtg
     1081 gagctccagg agcatgtcag cggcgatcgt acatccaccc accgacgcga tcggggaggt
     1141 cggtccggat ggtggctccg cccccctccc cggcatacga tgacgccgac gggccgcaga
     1201 gcggctatcc gggccgggtc cgtcatccga gctccggaga ccggaaggag ggaacgggga
     1261 catgaccctc aaccagggct tcggctacgt gttcggagcc atctacgccc tcgtgggcct
     1321 gctcggcttc accgtcaccg gcggggtcga gttcgccggc aaccagggcg agcagctgat
     1381 cggcttcgag ctcaacccgc tccacaacat cgtgcacatc gcgatcggcg ccctgctgat
     1441 cgccggggcc gccgggggcc tcagagcctc gcgcatgacc aacggctcag tgggcgcggc
     1501 gtacctgctg gtggggatcc tcgggatctt catggtgaac gaggactggg acttcctcgc
     1561 gctgaaccag gccgacaacg tccttcactt cgccacggcg gtgatcgccc tcgcgatcgc
     1621 gctgtgggcg gacaagaggg agcccgcccg agcctgaccg tcgaggccgg gcccgtcccc
     1681 ccatggggga cgggcccggc ctcacgacgg cagcgagaac ttcctggcca gccgggcgaa
     1741 gaaggattcc gcccccgcgt ggcgcaggag gcgggccggc cggtccgagc gccggatcct
     1801 gacacgggct cccagcggca gctccagcgc ctccctcccg tcgggagcga ggagccccgg
     1861 ctcgtctccc ttcacctcga tctgaacctc ctcgtcggga gcgaggacga gcggccggtc
     1921 gaacaccatg tggggcgaga ccggggtgag gatcaggcac tcgaccctcg gatggacgat
     1981 cgggccccgc gccgcgaacg agtaggccgt cgagcccgtg ggcgtggcca cgatcacccc
     2041 gtcgcccgag atcgtcgtaa cgtactcgcc cccgacgtgc acggcgaggg agatcacccg
     2101 ctggcgggcc cgtttctcca ccatgatctc gttgagcgcc cagggacgat cgctccacgg
     2161 cgccccctcg gcctcggcct ccaccgtgag ccgctcctcg acgacgagat ccccggccac
     2221 gaacgcgtcg agcatggcgg ggacctccgg gggctcgatg tcgctgagga accccagcct
     2281 ccccacgttc accccgagca cgggcgcccc cgcctcagag gccagcagcg ccgcccgcag
     2341 cagcgtgcca tctccgccca ccgagacgac gaggtccgcg ccgtccgcga gggagccggc
     2401 agcccgctcg ttcgcaccga cgtccacctc gtccagggac ctggtcgcga tccctcgctc
     2461 ccggcacgcc gtcacgatct cggcccccac ctcgagcgcc ctggggcggg ccggatggac
     2521 caccagcccc gctctcacga cggcacctcg gcgaccgccc gctccacgtc gagctcggca
     2581 ccctcgccgc ccaccctggc gtggagcagg aactcgacgt tcccggcggg cccccgcagc
     2641 ggcgaagcca cgacccccag cggcccggcc cccgcctcgc tcgcggccgc ggccacctcc
     2701 cggatcaccc gggcccacac ggccggatcg cgcacgacgc cgccgctccc cacgtcggcc
     2761 cggcccgcct cgaactgcgg cttcaccagc agcaccaggt cggcaccttc cgcggccacg
     2821 cccaccagcg cgggcagcgc cttcgtcaac gagatgaagg agaggtccgc gaccaagagc
     2881 tggggcggcc cctccagatg ctccggccgc aggtcccgaa cgttgaagcg ctcgaggacc
     2941 ctcacccggg gatcgttccg gatcgtccag gcgagctggc cgtagcccac atcgaccgcg
     3001 gtcacgtgcg ctgctcctcg ctgcaggagg acgtcggtga agccgccggt gctcgccccg
     3061 gcgtcgagca cgggtcggcc gcgtgggtcg atcccgaacg agtcgagcgc cgcggcgagc
     3121 ttccctccgc cccgcgagac gtacgctcgg ccctcgccct ccacggccag ggggtcttcc
     3181 ggggccacga gcgacgaaac cttgacggcc ggggcgccgc cgaccaggac ccgtcccgcc
     3241 tcgatcgcct cccgggcctc ggcgcgcgag gaagcgaggc cccgccggac cagctccacg
     3301 tcgagccgcc gcctcaagcc ttcggacggg agcgtctcgc cgaggaggtc ttcctcgtcg
     3361 aggcctgctt ccgcgtcgac gacttcgagg ccgacgactt cgaggaggac ttcgacctcg
     3421 acggcttctc agccttgtcc agccggcgct ccagcgtgcg gacccgcttc cgcagctccc
     3481 gcagctcgtc ctgcgtggcg gcccccgcga tccgcagctg tcgcttcacc tcgcgctgca
     3541 cgatcgcgac cacgcgctcg gtgttcctct tcgaccagtc cacgagctcc cgcgccagct
     3601 cctcggcccg ctcgggcttc agcgtgccca gccccgcctc cacgtaccta cgcacgacgt
     3661 ctcgcatgag ggaagcataa gacagagcag cgagcgtggt gccggtatac tcgggccgtt
     3721 ctccctttcc gtcccagaaa caggagcgtc gttggcgagc ctaggcgagg tggagcgcag
     3781 gctggaggag ctgctggggc ggctccgaga gacgaacggg gcgcacgtcg gcgcgtccct
     3841 cccggagccg cgcgtgctcg tcctcgaggt gagcgaccag gaggcgagct tctggaccga
     3901 gctgggcgac ggacgcgtgg gcgagcttcg ctccggccat cctgacgagg cgcacatccg
     3961 gatccgagcc ggctccgacg acatcgtggc cctcgcggac ggcgagctcg cgttcttccc
     4021 cgcctacctc tcgggacggg tccggatcga gggcggcgcc ctcgagatgc tgcagctccg
     4081 caggctgctc tgatgcccgg acgccgcttc atcggcagat cgctccgctc gctcggaggg
     4141 atgctggccc tgggccggct ggccacccgc ctcgacgaca acgggcccga ccccgccgag
     4201 ggtgaccacc tcggggcgat ccggggggag gtccggacgg tccggggccc tcgggcctcc
     4261 tcgctctaca ccgagtggtt ccctcccgcg gaggagggag gccccacgat catcttcacc
     4321 cacggcctct gcctgaccga ggccgtgtgg cactaccaga agcgggacct gtgcggcggg
     4381 gcgtacggcg cggtcacgtg ggacctcccc ggccacgggc actccgacag gatcgccccc
     4441 ggggagctga cccaagagat ggggatcgac acgctggccc gggtggtgga ggcgtacgcg
     4501 gagccgaacg gggtcgtcct cgtcggccac agcctgggcg ggaccctcac cctcgggtac
     4561 ctggcgcggc acgcggaggc ggcccgggag cgcgtcctcg gcgcggtgat cgtctccacc
     4621 ccgctggtgc agttcgctca ctcggtggcc ggccggtggc cgggggcggg gctcgaggcc
     4681 agagtcttgg ggaaggcggt gcgctacctg atcgagagcg acctcgtgga ccggaggctg
     4741 gccacggacg tggggaccga cctcgacacc ctctcctacc gagtcgtgcg cgtgggcttc
     4801 gggaagcacg cctcgcccac acaggtgcgc tacgtccgtg acgtgatcgc ctccgtcccg
     4861 ccccaggtcc gggccgacgc ctaccgggcc atgaccgcct acgacgtgcg ggcccacctg
     4921 ggccgggtgg agcacccgat cctcgtcgtg atcggcgagg aggaccgcct ggtggatccc
     4981 gaggagtcgc ggacgctcgc gacctccctg cccggggccg aggtgctcgg cctcgagggc
     5041 gtgggccacg cccccttcct ggaagccccc gagacgttca acgacgcggt gctggccttc
     5101 gcccgccggc tcagccggcc cgaggcggac acggcgtgat cccgatccgc gacgcgaatc
     5161 cggccacccg ccgcccctac gtcacgtggg cgttccttgc cctgaacgtg gcgctgttcc
     5221 tgctgtggca gccacggggc tcctgccccg gcgacctccg gttcacgacc caggccgagg
     5281 aggaggcctg cgccctcgag ctcgccttca ccttctgcca cggcgcgatc cccgaggagc
     5341 tgacctcgtt ccgcccgatg accgagctcc cggcgaccac gcaggatgcg cggttcgcct
     5401 cccagctcca ggagctgcgc tgcccggaga agggggtgct ggggtcgctg ttcagctcga
     5461 tgttccttca cggagggctg ctccatctgg gcgggaacat gctctacctc tggatcttcg
     5521 ggaacaacgt cgaggaccgc atgggccatc tcgtgttccc gctcttctac ctggcggggg
     5581 gggtggtggc cacctacgct cacgtggcca cggcgccctc ctccgccatc ccgctggtcg
     5641 gcgcctccgg cgcgatcgcc gcggtgctcg gggcctacct cgtgatctgg ccgcacgccc
     5701 gcgtgaccac gctggtgatc ttcttcttca tcacggtgat cgagctgccg gcggtggtgg
     5761 tcctcggcct ctggttcctc ctgcaggcct tccagggcgt agggagcctg ggcgtggacg
     5821 tgggcggggt ggcctggtgg gcgcacatcg gggggttcgc cttcggggcc atcgtggcct
     5881 ggctcttctt ccgggggaga cgcgagccc
//
LOCUS       DASUTZ010000120         2375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_31835, whole genome shotgun sequence.
ACCESSION   DASUTZ010000120 DASUTZ010000000
VERSION     DASUTZ010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31835"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..920)
                     /locus_tag="VF097_05425"
     CDS             complement(3..920)
                     /locus_tag="VF097_05425"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6262271.1"
                     /translation="MKIVSLLPSATEIVYALGLGDDLAAVTYECDYPPEAQEKPVVIE
                     TALPTDRPLSAGEIDAEVRAHMEQQAPIYKLDTDLIRQIDPDLILAQDICRVCAVPSG
                     HVQEALASLGSTAQVISLDPPDLEGIMEGILEVGKATGTEERGAELVAGMRERIERVK
                     AAGARLPRIRTLALEWMDPPFVGGHWIPQMVELASGITLLGEQGQPSRQVTWRAIADA
                     QPEVLVHMPCGYYLEEAEAEAQRLYEVPELRETTAVQAEAVVAVDATAYFSRPGPRIV
                     DGLEILAWCIHPEDYPQPPPDRAVRVPAP"
     gene            complement(917..2245)
                     /locus_tag="VF097_05430"
     CDS             complement(917..2245)
                     /locus_tag="VF097_05430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX6262272.1"
                     /translation="MAERVFNLPDLGEGLEDAEVVTWKVAEGDAVELNQPLVDVNTAK
                     ALVEIPSPFEGTVVTLHGGEGDVIAVGAPLVTFDVAEDAPVDESILEGAAAQEEEPEA
                     EKPKREAVLVGYGVDQERRGRRRRLRPPGQPTPPQAPAPTAPAPTPAPAQEPAATGKV
                     RASPVVRRLARDHGIDLSTVPGSGPGGRIRREDLQRTIEGDGGIAPAPAVAPGDEERV
                     PVRGIRRLIFQKMAKAWAEVPHVTTYRSIDATWVDALRRELTDDAVKVSPLPVVVRAL
                     VEVCRQHPKLNSSYDPETEEIVLKGSYHVGIATDTEQGLIVPVVRDADRKGIRQIARE
                     TAEIVQAVRAGAASPDQLTGSTITITNVGTFGSEYGTPIINIPEVAILALGAIRERPW
                     VVDGGLEPRPVCTLSLSFDHRIIDGAEAGRAMTDLAALLESPFRLGALPR"
     gene            complement(2250..>2375)
                     /locus_tag="VF097_05435"
     CDS             complement(2250..>2375)
                     /locus_tag="VF097_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020642407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX6262273.1"
                     /translation="LEAPVLRVTGWDTPYPPATLEEHYLPNVDRILGALERVLTY"
BASE COUNT          299 a          829 c          899 g          348 t
ORIGIN      
        1 cctcaggggg ccgggacgcg gacggcgcgg tcgggcgggg gctgcggata gtcctcgggg
       61 tggatgcacc aggccaggat ctccaggccg tccacgatcc gggggccggg acgtgagaag
      121 taggcggtgg cgtcgacggc cacgacggcc tcggcctgga cggccgtggt ctctcgcagc
      181 tcgggaacct cgtagaggcg ctgggcctcg gcctcggcct cctccaggta gtacccgcag
      241 ggcatgtgga ccaggacctc gggctgggcg tcggcgatcg cgcgccaggt gacctgcctc
      301 gagggttggc cctgctcgcc cagcagggtg atgcccgagg ccagctcgac catctggggg
      361 atccagtggc cgcccacgaa gggcgggtcc atccactcca gggccagggt gcggatacga
      421 gggaggcgag cgcccgccgc cttcacccgc tcgatcctct ctcgcatccc ggccacgagc
      481 tcggcgcccc gctcctcggt cccggtggcc ttccccacct cgaggatgcc ctccatgatc
      541 ccctcgaggt cgggggggtc gagcgagatc acctgcgccg tgctgccgag cgaggccagt
      601 gcctcctgga cgtgtcccga gggcaccgcg cagacgcgac agatgtcctg ggccaggatc
      661 aggtcggggt cgatctggcg gatcaggtcg gtgtcgagct tgtagatcgg cgcctgctgc
      721 tccatgtggg cccggacctc ggcgtcgatc tcgccggccg agagggggcg gtcggtgggg
      781 agggcggtct cgatcacgac cggcttctcc tgggcctcgg gcggatagtc gcactcgtag
      841 gtgacggcgg ccaggtcgtc gcccagcccg agcgcgtaga cgatctcggt cgccgacggg
      901 agcagcgaga cgatcttcac cgcgggagag cgccgaggcg gaagggactc tccaggaggg
      961 cggccaggtc ggtcatcgcc cgcccggcct cggccccgtc gatgatgcgg tgatcgaacg
     1021 agagcgagag cgtgcacacg ggtcgcggct cgagcccgcc gtccacgacc cagggccgct
     1081 ctcggatcgc gcccagggcg aggatcgcca cctcggggat attgatgatc ggcgtcccgt
     1141 actcggagcc gaaggtcccg acgttcgtga tcgtgatcgt gctgccggtg agctggtcgg
     1201 gcgaggcggc gcccgcccgg acggcctgga cgatctccgc ggtctcacgg gcgatctgac
     1261 ggatgccctt gcggtcggcg tcgcgcacga cggggacgat cagcccctgc tcggtgtcgg
     1321 tggcgatgcc cacgtgatag gagcccttga ggacgatctc ctcggtctcc ggatcgtacg
     1381 aggagttgag cttgggatgc tgcctgcaca cctcgaccag ggccctgacg acgaccggga
     1441 ggggggagac cttcacggcg tcgtcggtga gctcgcggcg gagggcgtcc acccaggtgg
     1501 cgtcgatcga gcggtaggtg gtgacgtggg gcacctcggc ccaggccttg gccatcttct
     1561 ggaagatgag gcgccggatc ccgcggacgg ggacgcgctc ctcgtcgccg ggggcgaccg
     1621 ccggggctgg cgcgatgccc ccgtcgccct cgatcgtgcg ctggaggtct tcgcgtcgga
     1681 tccgaccgcc gggcccggag cccgggacgg tcgagaggtc gatgccgtgg tcgcgggcca
     1741 ggcggcggac gacgggcgag gccctgacct tgccggtcgc ggcgggctcc tgcgcgggcg
     1801 ccggcgtcgg ggccggagcg gtcggagcgg gggcctgggg cggcgtcggc tggcccggag
     1861 ggcgcagccg ccgccgccgc ccccggcgct cctggtccac gccgtagccc acgaggaccg
     1921 cctcgcgctt cggcttctcc gcctcgggct cctcctcctg cgccgcggcc ccctcgagga
     1981 tcgactcgtc caccggcgcg tcctcggcca cgtcgaacgt cacgagcggc gccccgaccg
     2041 cgatcacgtc gccctcgccc ccgtgcagcg tgacgaccgt tccctcgaac ggggagggga
     2101 tctcgacgag cgccttggcc gtgttcacgt ccaccagcgg ctggttgagc tcgaccgcgt
     2161 cgccctcggc caccttccag gtgacgacct cggcgtcctc cagcccttcg ccgaggtccg
     2221 ggaggttgaa gactcgctcg gccatggcct cagtacgtca ggacgcgctc cagcgcgccc
     2281 aggatccggt cgacgttcgg caggtagtgc tcctcgaggg tggccggcgg gtacggcgtg
     2341 tcccaccccg tgacccgcag gaccggggcc tcgag
//
LOCUS       DASUTZ010000121        13729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_550268, whole genome shotgun sequence.
ACCESSION   DASUTZ010000121 DASUTZ010000000
VERSION     DASUTZ010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13729
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550268"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VF097_05440"
     CDS             complement(<1..585)
                     /locus_tag="VF097_05440"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6262274.1"
                     /translation="MGQRILITGIAGHLAGELARRLEDDTEVEYLVGVDLVEPRADLT
                     RLEYVRADIRNPLVVKVLQTTEVDTVVHLSLLSTPHGVGGRSNMKEINVIGAMQLFAA
                     SQRAEAVRKIVMKSTTAVYGSGPKDPAVFTEDMSARSEAPHGYAKDAIEVEAYARSFA
                     RRRDDVDLTVLRFANFIGPGVASTLTRYFSLPLIP"
     gene            complement(587..1405)
                     /locus_tag="VF097_05445"
     CDS             complement(587..1405)
                     /locus_tag="VF097_05445"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEX6262275.1"
                     /translation="MTRPILFLSDYGLADEFVGVCHAVMVRIAPGSPIIDLAHEIPPQ
                     DVPRGAAVLRQAARHGPRNGVYVAIVDPGVGTERRPVAIQAGDAFLVGPDNGVLSTAW
                     TELGGARRAFKISSREVMLDKVSPTFHGRDVFSPCAAHLSNGMSIEEVGDEVDPATLV
                     EVALPGARIEPGYVHCKVIGIDRFGNVQLSARPDDLSEAGIPSDDLIHIRAGGRGYDV
                     AVRETFAHVEEGEGVVVVDSAGFLTLAKNRRHAAKELGIRVGDHVVLAPVTPGA"
     gene            1453..1755
                     /locus_tag="VF097_05450"
     CDS             1453..1755
                     /locus_tag="VF097_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262276.1"
                     /translation="MGTSENLEFTDWAKDILGRSQEAARRFNPRALIRLVLEDGAMEA
                     RLTEEPEAGDREVDAGGVTVLVAPGIEGLVDVEEPHDRIVLRPAGSTPNPRGHHES"
     gene            complement(1743..2426)
                     /locus_tag="VF097_05455"
     CDS             complement(1743..2426)
                     /locus_tag="VF097_05455"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6262277.1"
                     /translation="MRRTALLLLILVAACGRGATPEPGEVIRFETSDGVELVGELRGR
                     GTNGVVLGHMYGSSREAWAAFAQQAAEAGYLTLAFDLRGFGESGGTSSAPDAPTDLRA
                     AVEAIREHGAREVVVIGASFSGTGALVAAGEEEWAGVVTLSAPASFEGLRADAAVVEA
                     VDEPKLFIAAQGDGSASGSAQGFYQAASGAKRVEIVVGDEHGTDLLESPRAQLVRDLL
                     MAFLRAQLS"
     gene            complement(2428..3318)
                     /locus_tag="VF097_05460"
     CDS             complement(2428..3318)
                     /locus_tag="VF097_05460"
                     /inference="COORDINATES: protein motif:HMM:NF027889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp1/GlmU family protein"
                     /protein_id="HEX6262278.1"
                     /translation="MDPHEEAIRRVVAGRGVTLLVGGLDAGKTTLARRIARAGLSSGL
                     TVGFLDADVGQSTVGPPTAVGLRICRQLSDLEHDSLARADVLAFVGSTSPQGHLLPLV
                     VGTRMLLDRALAEGCDLVVIDTTGLVSGVYGQLLKGYKVQLLQPDHVIGVERGSELAP
                     VLGVARRFSAAQVESLKVHPDVVPTSVEQRALNRQESLKRAFAGPLQRWRVKPTVFLP
                     TLPDLFDLAGLDRIVVGLSDGKGGTIGIGYLEHVAEDGGLRLVSPATEGPKALVLGSV
                     RLEEDFRSRRVELRNLLGSD"
     gene            complement(3368..3568)
                     /locus_tag="VF097_05465"
     CDS             complement(3368..3568)
                     /locus_tag="VF097_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6262279.1"
                     /translation="MAPDRSFREAQFVTVQEVAQLMRVSKMTVYRLIKQGDLPAVRIG
                     RGYRLREEDVHSYLDSRYTEAG"
     gene            3627..4325
                     /locus_tag="VF097_05470"
     CDS             3627..4325
                     /locus_tag="VF097_05470"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX6262280.1"
                     /translation="MLYVGTSGFAYDEWRGTFYPEDLKRDAYLEHYATRFPAVEINYT
                     FRRFPSPKTLERWRGQSEDGFRFVLKAHGRITHTRRLRDTDEDVRSFLELARLLGDRL
                     GPILFQCPPNLPYDESLLAAFLGYLPPDVRTAFEFRHPSWEKAKPLLASQGAAWVDSE
                     TDEKAVDEIAPGPFAYLRLRRDDYSEEELEAWADRVAEAVGAGRDVYCFLKHEQEAFR
                     RADRLRDLVGARVG"
     gene            complement(4329..5132)
                     /locus_tag="VF097_05475"
     CDS             complement(4329..5132)
                     /locus_tag="VF097_05475"
                     /inference="COORDINATES: protein motif:HMM:NF027034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HEX6262281.1"
                     /translation="MSKPHSGYPSEDWEPAEHPREILIRALLDGGVAGVNSHDRPNVL
                     WKIQRLCEGDPDLQFGLTGVDGMAPGDVLDLMAREGGFDGDPSLKHGLIMVDPEPVLR
                     GCEAIGDRLALAAERGESVLLATGHPSGLPLLYQATGRLLVEHGAKLLRPLEGFTWKE
                     RGRNRQIRYMAGVAVYTDRASALHTHSPEPMRRMLEEVTPDLVFADHGFAGAAIEAGI
                     DTVSIADVNDPALVVAWRRGRGGPVVVMDDNVQPDAYWPCFQAIASRFS"
     gene            5161..5928
                     /locus_tag="VF097_05480"
     CDS             5161..5928
                     /locus_tag="VF097_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6262282.1"
                     /translation="MPGLDGRVALVTGGSKGIGKAIALALAEDGADVALAARGEEDLA
                     TAAKEVEALGRRALPVPTDVTDPEQVGTLVRRTIDELGGLDVLVNNAGAAPFLSTVES
                     IRPEGFEKYFRVNFHSAFYATRAAAGHLLKQGSGSVVNVASVAAFIASPGLTYYAGAK
                     AALIAFTKTVAQEWAPHGIRVNAVAPGWIETDLNAYSRQDPGFVESTRAQIPMGRWGT
                     AEEVAAVVRFLASDAASFMTGSVVVVDGGQTLTALGG"
     gene            5928..6680
                     /locus_tag="VF097_05485"
     CDS             5928..6680
                     /locus_tag="VF097_05485"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6262283.1"
                     /translation="MATKVAVVTGASSGIGEATARSLAREGLRLVLAARREDRLRAMA
                     EELDAEVLPVRCDVTLKKDLRAVVAATEERFGRCDILINSAGVPGRGRFADVDLGPLE
                     AVTFTNYVGLLRATKLFLPMLLEARGHMVNVASLAGLFALPGAEAYAASKHAVVALSE
                     SLSLSFGDALKVTAVCPGFVNTEGFPQPDKPRPVTVPLEAVAEGIVDVLRRGRTGMVA
                     IPAWPGPVSALQRLVPGLYRRAVRRGVGRFRR"
     gene            complement(6677..7351)
                     /locus_tag="VF097_05490"
     CDS             complement(6677..7351)
                     /locus_tag="VF097_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014396919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX6262284.1"
                     /translation="MRIGVLGTGSVGRVLGGKLAELGHEVMLGTRDVEALTTRPAGER
                     ESESFSEWMDRTPGAAVGTFAEAAAHGEMLFNCTRGEGSLDALASADHRQVGGKVLVD
                     VSNPLVFEEGAMKLFVPSGDSLAERIQRQHPHAKVVKALNTVNARVMVDPAGVGGGDH
                     TIFVCGDDDGAKDAVGEILRAFGWKHIMDLGDLSAARGMEAYLLLWIRMMGTVGSPAL
                     NVKVVT"
     gene            complement(7363..8505)
                     /locus_tag="VF097_05495"
     CDS             complement(7363..8505)
                     /locus_tag="VF097_05495"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX6262285.1"
                     /translation="MRIGILTGGGDVPGLNACIKAVVERVADQGHEVVGIRRGWDGLL
                     STNLDEPDTVAPNLRHLDRAAVRTIDRTGGTVLHTSRTNPGKVRAQDAPSFLEPGGEE
                     PFDFTPHVLRVLESQGIEVLIPIGGDDTLSYALRMHEEGVPVIAIPKTMDNDVHGTDY
                     CIGFSTAVTRGVQFIHNLRTSTGSHERIAVVELFGRYSGETSLITAYLAGVDRAAISE
                     VPVDVERLAGLLVEDKRANPSNYAMLTISEGAVIQGGDLMLSGEEDAYGHRKLGGIGS
                     QVAQLIKRFTGEGIVEQQLGYLVRSGSPDSLDLMVATNYAVMAADLALEGSSGRMVAL
                     RGGTYTNVPISVTREGVKRVDVDELYDVEQYRPKLRHVGGKPMFLY"
     gene            complement(8546..8989)
                     /locus_tag="VF097_05500"
     CDS             complement(8546..8989)
                     /locus_tag="VF097_05500"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6262286.1"
                     /translation="MSGFSIRDALPEDADDVARLMAFVGGTIPAGDHRARPGLESFLS
                     QDGAVLVAESEGRVVGACTLLFTRFNPMDAAPGAWLDGLAVEESARSRGIGRALMEEA
                     RRRAEGAGCDSIVLHTHEDQGAALALYGSLDMKRHGILMVWPLSR"
     gene            complement(9008..9718)
                     /locus_tag="VF097_05505"
     CDS             complement(9008..9718)
                     /locus_tag="VF097_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262287.1"
                     /translation="MTPLDDYRDEMATLSSEQIEGVLAGRPVDAPDAEPVARLARDLR
                     ERLLVDLHGEIEARHLAAMRAEVAPVPRRTRPRRVATRLALAAALVLGAGAAAATTAR
                     LTGSPSIAPEGLPIPAHGDSRTQQPTSELDPGSAEPADRPSGPVADTIRAPSARWKGC
                     GAAPRVACPKGFSKSRGTGKGERAHDDRRGPRGHGKGKGLGPGAGPPPGKPEGPGPGA
                     PPGGAPPGDGPPPQTPGR"
     gene            complement(9715..10263)
                     /locus_tag="VF097_05510"
     CDS             complement(9715..10263)
                     /locus_tag="VF097_05510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6262288.1"
                     /translation="MPPFEEVLDAARAGEDWALEAIYRDLAPAVAGYLRGQRAGEPED
                     LTSDVFVGIVRGLRAFSGDERAFRSWVFSIAHRRLLDERRRLARRRERPVDPGELAAE
                     GPRGNVEAEGLERLGAGWAAELLDTLTDEQRTVVLLRIVADLSVREVADVTGRSPGAV
                     KTMQRRALLQLARRVEREGVSP"
     gene            complement(10308..11708)
                     /gene="pyk"
                     /locus_tag="VF097_05515"
     CDS             complement(10308..11708)
                     /gene="pyk"
                     /locus_tag="VF097_05515"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX6262289.1"
                     /translation="MTRATKLVCTLGPASADRVPELVEAGMDVARINLTHGTREDHER
                     LLRSVREASREAGRAVAVMTDLSGPKVRLGELEPEAVRLQDGGVFVLRGGKGPGDARG
                     VPTSHAGLPDDLEVGDRVLLSDGVVELRVEETTGDEVQTRVVRGGLIRSRAGVNVPSG
                     RLSVPAITEKDERDLAWVGSAGIDLVAQSFVRHAAEVRALADLLGDGPALLVAKIETR
                     SAVEESDGILEAADVVMVARGDLGVELPPERIPVIQKDLVYRANRSGVPAVVATQMLE
                     SMTGSRRPTRAEVSDVAGAAFDGAAAILLSAETAIGRHPVEAARTAAAILETAEREGA
                     RFLSATVEERVEGSDAWAVADAAATAAADSGAVAVACFTRTGLTARLLSGVRLPVPVY
                     AYSHDDAVVRRLTVFRGVHPRPCDPPEDTDAMLTMMDRRLREEGLASSGDPVVLVAST
                     PVGRSRTNLLKVHRVG"
     gene            complement(11705..12835)
                     /locus_tag="VF097_05520"
     CDS             complement(11705..12835)
                     /locus_tag="VF097_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HEX6262290.1"
                     /translation="MSDRVALVYSPEVVEYDHGPQHPLKPVRVLLTRELIAAYGVLEA
                     GHVDEVPSRLASDQEVGLVHTQAYIDVVKAAGSGERGDWWAYGFGPGDNPIFPAMHEA
                     SARVVGASIEAAGLVLSGEADHAFNPAGGLHHAMPERASGFCVYDDPAVAIRWLLDRG
                     VERIAYVDVDVHHGDGPQAIFYDDPRVLTISLHESGRYLFPGTGFVSERGEGDAEGSK
                     VNVPLPPWTSDEAWLRAFREIVPPLVRRFHPDVLVTQLGCDTHHTDPLAHLALSTRAY
                     REASVELHDLAHGVAGGRWVATGGGGYQWARVVPRAWAIYFAEMAGVELPDDLPEGWR
                     QLAAEHAGGPMPERLSEEPPEVPAGSGEVRSVVDDVKRAFSV"
     gene            complement(12852..>13729)
                     /locus_tag="VF097_05525"
     CDS             complement(12852..>13729)
                     /locus_tag="VF097_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6262291.1"
                     /translation="VHGFGEEAGAPMVEHPDIRAISFTGSLEVGRKINERCGALMKRC
                     SLELGSKNVMIVMPDAEMDLAVEAASWGAFATSGQRCTATSRLIVHDDAREEFTERML
                     KRVEGMKVGSGLDPEVELAPVINSKQKERILEYIEIGKKEGAKLLTGGEELTGDGYEK
                     GNFIAPAVFDEMTPDMRIAQEEIFGPVTGIIRTDDVDEAIRMANSVDYGLSAAIYTHD
                     ITNVFEAVTQLEFGIVYVNAPTIGAEIQLPFGGMKNTGNGHREAGPQALDEFMEWKAV
                     SVDYSGKVQKAQGID"
BASE COUNT         1934 a         5058 c         4798 g         1939 t
ORIGIN      
        1 ggggatcaac gggagggaga agtagcgcgt gagggtcgag gccaccccgg gcccgatgaa
       61 gttggcgaag cgaaggacgg tgaggtccac gtcgtcccgg cgacgagcga agctccgcgc
      121 gtaggcctcg acctcgatcg cgtccttcgc gtagccgtgc ggggcctcgg agcgcgcgct
      181 catgtcctcg gtgaacaccg ccgggtcctt cggtccggag ccgtagaccg ccgtggtcga
      241 cttcatcacg atcttccgca cggcctccgc gcgctgggag gccgcgaaga gctgcatcgc
      301 cccgatgacg ttgatctcct tcatgttgct gcggccgccc acaccgtggg gcgtggagag
      361 caggctgaga tgcacgacgg tgtcgacctc ggtggtctgg agcaccttca ccacgagggg
      421 gttccggatg tcggcccgga cgtactccag ccgggtcagg tcggcccgag gctcgacgag
      481 gtccacgccg accaggtact cgacctcggt gtcgtcctcg agccgccggg ccagctcccc
      541 ggccaggtgc ccggcgatcc ccgtgatcag gatgcgctgg cccacgtcag gcccccggcg
      601 tcaccggggc cagcacgacg tgatcgccca cgcggatgcc gagctccttc gccgcgtggc
      661 ggcggttctt ggccagcgtg aggaaccccg cggagtccac gaccacgacc ccctcgccct
      721 cctcgacgtg ggcgaacgtc tcccgcacgg ccacgtcata ccctcggccg ccggcccgta
      781 tgtggatcag gtcgtcggag gggatgccgg cctccgacag gtcgtcgggg cgcgccgaga
      841 gctggacgtt gccgaagcgg tcgatgccga tcaccttgca gtggacgtag cccggctcga
      901 tccgggctcc ggggagcgcc acctcgacca gcgtcgcggg gtcgacctcg tctccgacct
      961 cctcgatcga catgccgttc gagaggtgcg ccgcgcacgg cgagaacacg tcgcgcccgt
     1021 ggaacgtggg cgaaaccttg tcgagcatca cctcccgcga ggagatcttg aacgcccgac
     1081 gagcgccgcc gagctcggtc cacgcggtcg agaggacgcc gttgtcgggt ccaacgagga
     1141 acgcatcccc cgcctggatc gccaccggcc gccgctcggt gccgacgccg gggtccacga
     1201 tcgccacgta gacgccgttc cgcggcccgt gacgggcggc ctgcctgagc accgcggcgc
     1261 cccggggcac gtcctgaggc gggatctcgt gtgccaggtc gatgatcggg ctgccgggcg
     1321 cgatccggac catcaccgcg tggcagacgc cgacgaactc gtcggccagc ccgtagtccg
     1381 agaggaacag gatcggtctc gtcatgaacg accggaaggt accagagccg gggtgatagc
     1441 gtggaccgcc ggatgggaac gagcgagaat ctcgagttca ccgactgggc caaggacatc
     1501 ctggggaggt cccaggaggc cgcgcgtcgc ttcaatccgc gagcgctcat ccggctcgtc
     1561 ctcgaggacg gggccatgga ggccaggctc accgaggagc ccgaggccgg ggaccgcgag
     1621 gtcgacgccg gcggcgttac cgtcctcgtc gcccccggga tcgaggggct cgtggacgtc
     1681 gaggagccgc acgaccggat cgtcctccgc cccgccggct cgacacccaa cccgcgcggc
     1741 catcacgaga gctgagccct gaggaaggcc atcagcaggt cccgcacgag ctgggcccgg
     1801 gggctctcca gcaggtcggt gccgtgctcg tcccccacca cgatctcaac gcgcttggcc
     1861 cccgaggcag cctggtagaa gccctgggcg gagccggagg ccgagccgtc gccctgcgcc
     1921 gcgatgaaga gcttcggctc gtccaccgcc tcgaccacgg cggcgtcggc ccgcagcccc
     1981 tcgaacgagg ccggcgccga cagcgtgacc acgccggccc actcctcctc gcccgccgcc
     2041 accagcgcgc cggtccccga gaagctggcg cctatcacca ccacctctcg cgctccgtgc
     2101 tcgcggatcg cctcgaccgc cgcgcggaga tcggtcggcg cgtcgggggc gctgctggtc
     2161 ccgccggact cgccgaagcc ccggagatcg aaggcgaggg tcaggtagcc ggcttcggcc
     2221 gcctgctggg cgaaggcggc ccacgcctcg cggctcgagc cgtacatgtg gccgaggacg
     2281 accccattcg tgccccgccc gcgcagctcg cccacgagct cgaccccgtc cgacgtctcg
     2341 aaccggatca cctcgcccgg ctcgggcgtc gccccgcgcc cgcaggcggc gacgaggatg
     2401 agcaggagca gggccgtccg acgcatgtca gtccgagccg aggaggttcc gcagctccac
     2461 ccgccggctg cggaagtcct cctcgagacg gaccgagccg agcacgagcg ccttcggccc
     2521 ctcggtggcg ggcgagacga gccgcaggcc cccgtcctcg gccacgtgct ccagatagcc
     2581 gatcccgatc gtccctccct tcccgtccga tagcccgacg acgatccggt cgagcccagc
     2641 cagatcgaag aggtcgggga gggtggggag gaaaacggtc ggcttcacgc gccagcgctg
     2701 cagcggcccg gcgaaggcgc gcttcagcga ctcctgacga ttgagggctc gctgctcaac
     2761 cgacgtcggg accacatcgg ggtggacctt cagggactcg acctgggcgg cgctgaagcg
     2821 gcgcgccacg cccagcacgg gggccagctc gctcccccgc tcgaccccga tcacgtggtc
     2881 cggctggagc agctgcacct tgtagccctt caggagctgg ccgtacacgc ccgagacgag
     2941 cccggtggtg tcgatcacca cgaggtcgca accctctgcg agcgcgcggt cgagcagcat
     3001 gcgcgtgccc acgaccaacg ggaggaggtg gccctgggga gacgtcgatc cgacgaacgc
     3061 caggacgtcg gcccgggcca gcgagtcgtg ctcgagatcg gagagctggc ggcagatccg
     3121 caggcccacc gcagtgggag ggcccacggt cgactgcccc acgtcggcgt cgaggaaccc
     3181 caccgtgagc ccggacgaga ggcccgcgcg cgcgatccgt cgcgcgaggg tggtctttcc
     3241 ggcatccagg cctcccacca gcagcgtcac gccgcggccg gcgacgaccc gccggatggc
     3301 ctcctcgtgt ggatccatct ccgccctcga acggtcgcgc cgctcgagaa gctcggatcc
     3361 gtgcgaacta ccccgcctcc gtgtacctcg aatccaggta gctgtgcacg tcctcctcgc
     3421 gcaatcggta cccccggccg atgcgtacgg ccggcaggtc cccctgcttg atcaatcggt
     3481 acacggtcat cttggacacc cgcatgagct gggcgacctc ttgcacggtc acgaattgag
     3541 cttctcggaa cgatctgtca ggggccacgc tggcgcgatt gtagcccagc cctcgctcca
     3601 cgggccagcc gtgattggat accggcatgc tgtacgtggg gacgtccggg ttcgcctacg
     3661 acgagtggcg ggggaccttc tacccggagg acctgaagcg cgacgcgtac ctcgagcact
     3721 acgcgacgcg gttccccgcc gtggagatca actacacgtt ccggagattc ccatcgccga
     3781 agacgctgga gcggtggcgg ggacagagcg aggacgggtt ccggttcgtg ctgaaggcgc
     3841 acgggcgcat cacccacacg aggaggctcc gagacacgga cgaggacgtg cggtcgttcc
     3901 tggagctggc ccggctgctc ggggaccggc tggggccgat cctcttccag tgcccgccga
     3961 acctcccgta cgacgagtcg ctgctggcgg cgttcctcgg ctacctgccg ccggacgtgc
     4021 ggactgcgtt cgagttccgg cacccgtcgt gggagaaggc caagccgctc ctggcgtccc
     4081 agggcgcggc gtgggtggac tcggagacgg acgagaaagc cgtggacgag atcgcgcccg
     4141 ggccgttcgc ctacctgcgc ctgcggaggg acgactactc cgaggaggag ctggaggcgt
     4201 gggctgaccg ggtcgcggag gcggtcggcg cgggccggga cgtgtactgc ttcctcaagc
     4261 acgagcagga ggccttccgc cgcgccgacc gcctccgcga cctcgtcggc gcgcgcgtgg
     4321 gctgacgcct acgagaatcg ggaggcgatg gcctggaagc agggccagta ggcgtcgggc
     4381 tggacgttgt cgtccatcac cacgaccgga ccccctcgtc cccggcgcca cgccacgacc
     4441 agcgccgggt cgttcacgtc ggcgatcgag accgtgtcga tccccgcttc gatcgcggcc
     4501 ccggcgaacc cgtggtcggc gaagaccagg tcgggcgtga cctcctcgag catccgacgc
     4561 atcggctcgg ggctgtgcgt gtggagcgcg gacgcgcggt ccgtgtacac ggccacgccg
     4621 gccatgtatc ggatctgccg gttgcggccg cgctccttcc aggtgaagcc ctccagcggg
     4681 cgcagcagct tggcgccgtg ctcgacgagc agccgaccgg tggcctggta gaggaggggg
     4741 agcccggagg ggtgcccggt ggccagcaac accgactcgc cccgctcggc ggccagcgcc
     4801 aggcggtcgc cgatcgcctc gcacccacgg agcacgggct cggggtccac catgatcagc
     4861 ccgtgcttca gactcgggtc gccgtcgaag cctccctccc tcgccatgag gtccaggacg
     4921 tcgccagggg ccatgccgtc cacacccgtc agcccgaact ggaggtccgg gtcgccctcg
     4981 cagaggcgct ggatcttcca gagcacgttg ggccggtcgt gcgagttcac tcccgccact
     5041 ccgccgtcca ggagcgcccg gatcaggatc tcgcgcggat gctccgcggg ctcccagtct
     5101 tcgctcgggt agccggagtg tggtttgctc atcgggccat cgtatacgga ggtctcgcgg
     5161 atgcctgggc tggacggcag ggtcgccctc gtgacaggcg gctcgaaggg gatcgggaag
     5221 gccatcgcgc tggcgctggc cgaggacggc gccgacgtgg cgctggccgc ccggggtgag
     5281 gaggacctgg ccaccgcggc gaaggaggtc gaggcgctgg gccgtcgcgc gctccccgtc
     5341 cccaccgacg tcaccgatcc cgagcaggtg gggacgctcg tgcgtcggac gatcgacgag
     5401 ctgggcggcc tcgacgtcct ggtgaacaac gcgggggcgg ccccgttcct gtccacggtg
     5461 gagtcgatcc ggcccgaagg gttcgagaag tacttccgcg taaacttcca ttcggcgttc
     5521 tacgccaccc gggccgcggc ggggcacctc ctgaagcagg gctcgggctc ggtggtcaac
     5581 gtggcgtcgg tcgccgcgtt catcgccagc cccgggctga cctactacgc gggggcgaag
     5641 gcggcgttga tcgccttcac gaagacggtg gcccaggagt gggcgcccca cgggatccgg
     5701 gtgaacgcgg tggcccccgg gtggatcgag accgacctca acgcgtactc gcgacaggac
     5761 cccggtttcg tcgagagcac ccgggcccag atcccgatgg ggcgatgggg gacggccgaa
     5821 gaggtggcgg cggtcgtgcg gttcctggcc tcggacgccg cctcgttcat gaccgggtcg
     5881 gtggtggtcg tggacggagg acagacgctc acggccctcg gcggctgatg gcgacgaagg
     5941 tcgccgtcgt cacgggcgcc tcgtcgggga tcggcgaggc gacggcccgg tcgctggccc
     6001 gggaggggct gcgtctggtg ctggcggcgc ggcgcgagga ccgcctgcgg gccatggccg
     6061 aggagctcga tgcggaggtc ctgcccgtgc ggtgcgacgt cacgttgaag aaggacctgc
     6121 gggcggtcgt ggccgcgacc gaggagcggt tcgggcgctg cgacatcctg atcaacagcg
     6181 cgggcgtgcc ggggagggga cggttcgcag acgtcgacct cggcccgctc gaggcggtga
     6241 ccttcaccaa ctacgtcggg ctgctgcggg caacgaagct gttcctgccg atgctgctgg
     6301 aggcgcgcgg gcacatggtg aacgtcgcct cgcttgccgg gctgttcgcg ctccccggag
     6361 cagaggccta cgccgcgtcg aagcacgccg tggtggcgtt gtccgagtcg ctctcgctct
     6421 cgttcggcga cgcgctcaag gtgacggcgg tctgccccgg cttcgtgaac acggagggat
     6481 tcccgcagcc ggacaagccg cgccccgtga cggtgcccct ggaggcggtg gccgagggga
     6541 tcgtggacgt gctccgccga gggcgaacgg ggatggttgc gatcccggct tggccgggtc
     6601 ccgtctcagc gctccagcgt ctcgtcccgg gcctctaccg gcgcgccgtc cgccgcggcg
     6661 tcggccgatt ccgccgctag gtgacgacct tgacgttgag ggccggcgag cccacggtcc
     6721 ccatcatgcg gatccatagg agcaggtagg cctccatacc gcgcgcggcc gaaaggtcac
     6781 cgaggtccat gatgtgcttc cagccgaagg cgcgcaggat ctcaccgacg gcgtccttcg
     6841 ccccgtcgtc gtcgccgcac acgaagatcg tgtggtcgcc gccgcccacc ccggcagggt
     6901 ccaccatcac ccgcgcgttc acggtgttga gtgccttcac cactttggcg tgcggatgct
     6961 gacgctggat ccgctcggcg agggagtctc cgctcggaac gaagagcttc atcgcgccct
     7021 cctcgaacac gagggggttg gagacgtcga ccagcacctt gcccccgacc tggcggtggt
     7081 cggcggaggc cagcgcgtcg agggacccct ccccacgcgt gcagttgaag agcatctcgc
     7141 cgtgggcggc cgcctcggcg aacgtcccga cggcggcccc cggcgttcga tccatccact
     7201 cggagaacga ctcgctctcc cgctcccccg ccgggcgcgt cgtgagcgcc tcgacgtccc
     7261 gcgtgcccag catgacctcg tgcccgagct cggccagctt cccgccgaga acgcgcccca
     7321 ccgatcccgt ccccagcaca ccgatccgca tccaggcctc cctcagtaca ggaacatcgg
     7381 cttcccaccg acgtgacgga gcttgggccg atactgctcc acgtcgtaca gctcgtccac
     7441 gtccacccgt ttgaccccct cgcgggtcac cgagatgggg acgttcgtgt aggtgccgcc
     7501 gcgcagcgcc accatgcgac ccgacgaccc ctcgagcgcg aggtccgccg ccatcaccgc
     7561 gtagttcgtg gccaccatga gatcgagtga gtcggggctg cccgagcgga cgaggtaccc
     7621 gagctgctgc tcaacgatcc cctcgccggt gaaccgcttg atcagctgcg cgacttgcga
     7681 gccgatcccg cccagcttcc ggtggccgta ggcatcctcc tcgcccgaga gcatgaggtc
     7741 gccgccctgg atcacggctc cctcggagat cgtgagcatc gcgtagttgc tggggttcgc
     7801 ccgcttgtcc tcgacgagga gccccgccag ccgctcgacg tcgacgggga cctcggagat
     7861 cgcggctcgg tccaccccgg ccagatacgc ggtgatcagc gaggtctcgc cgctgtagcg
     7921 accgaacagc tcgacgaccg cgatgcgctc atgcgaaccc gtgctggtgc ggaggttgtg
     7981 gatgaactgg acgccgcgcg tcacggcggt ggagaagccg atgcagtagt cggtcccgtg
     8041 aacgtcattg tccatcgtct tcgggatggc gatgacgggg accccctcct cgtgcatccg
     8101 gagcgcgtac gagagcgtgt cgtcgccccc gatcgggatg agcacctcga taccctgcga
     8161 ctcgagcacc cgcaggacgt gcggggtgaa gtcgaacggc tcctccccgc ccggctccag
     8221 gaacgagggc gcgtcctgcg ctcgcacctt gcccgggttc gtccgggaag tgtggagcac
     8281 ggtgccgccg gtacggtcga tcgtgcggac ggccgctcgg tcgaggtgcc ggaggttggg
     8341 cgcgacggtg tcgggctcgt cgaggttcgt ggagaggagg ccgtcccagc cgcggcggat
     8401 gcccacgacc tcatggccct ggtcggccac gcgctcgacc acggccttga tgcaggcgtt
     8461 gagccccggg acgtcgcccc cgcccgtcag gatcccgatg cgcatgggtc cctcctccct
     8521 gccgacccgc ccatcgtagc gggggtcaac gcgacagcgg ccacaccatg aggatgccgt
     8581 ggcgcttcat gtccaacgac ccgtagaggg cgagggcggc gccctggtcc tcgtgcgtgt
     8641 ggaggacgat cgagtcgcag cccgctccct cggcccggcg gcgggcctcc tccatcagag
     8701 cccggccgat accgcggctg cgggcgctct cctccacggc gaggccgtcg agccaggcgc
     8761 ccggggcagc gtccatcggg ttgaagcgcg tgaacaggag cgtgcacgcg ccgaccacgc
     8821 gcccctccga ctcggccacc aggaccgcgc catcctgtga gaggaagctc tccaggcccg
     8881 ggcgggcgcg gtggtcgccg gcagggattg tcccccccac gaaggccatc agccgggcga
     8941 cgtcgtcggc gtcctcgggc agggcatcac ggatcgagaa gccgctcatc cgcgaagcct
     9001 aaccgagcta acggccgggg gtctgcgggg gcgggccatc accgggcggc gcgcccccgg
     9061 gcggcgcgcc gggtcccggg ccttcgggct tccccggagg cggcccggcg cccggcccga
     9121 ggcccttccc cttgccgtgg cccctcgggc cgcgccggtc gtcgtgggcg cgctctccct
     9181 tgcccgtccc cctgctcttc gagaacccct tagggcaggc gacgcgcggg gccgccccac
     9241 agcccttcca gcgggccgag ggcgctcgga tcgtgtcggc gaccggcccc gatgggcggt
     9301 cggccggctc ggcgctcccc ggatcgagct cgctcgtcgg ctgctgggtg cggctgtcac
     9361 cgtgggccgg gatcggcaga ccctcgggcg cgatggaggg cgatcccgtg aggcgcgcgg
     9421 tggtggccgc ggcggctccc gcgccgagca cgagggcggc cgccagagcg agcctcgtcg
     9481 ccacccgccg cggccgggtg cgtcgcggga ccggcgcgac ctcggcccgc atggcggcga
     9541 ggtgccgcgc ttcgatctcg ccgtggagat cgacgagcag ccgctcgcgg aggtcccgtg
     9601 cgagccgggc gacgggttcg gcgtccggcg cgtcgaccgg acggcccgcc aggaccccct
     9661 cgatctgctc ggagctcagg gtcgccatct cgtcccggta atcgtcgagc ggcgtcacgg
     9721 ggaaaccccc tctcgctcga ccctgcgcgc gagctgaagg agcgcccgcc gctgcatcgt
     9781 cttcacggcc cccggcgacc tcccggtgac gtcggccacc tccctgaccg agaggtcggc
     9841 cacgatccgg agcagcacca cggtccgctg ctcgtcggtc agggtgtcga ggagctcagc
     9901 ggcccagccg gccccgagcc tctccaggcc ctcggcctcg acgttccccc tcggcccctc
     9961 ggccgccagc tcccctgggt cgacgggccg ctcccggcgc cgagccaggc ggcgccgctc
    10021 gtcgaggagc cggcggtggg cgatcgagaa gacccagctc cggaacgccc gctcgtcgcc
    10081 ggagaacgcg cggagccccc gcacgatccc cacgaagacg tccgaagtca ggtcctcggg
    10141 ctcgcccgcc cgctggccgc ggaggtaccc ggccaccgcg ggagcgaggt cacggtagat
    10201 cgcctccagg gcccaatcct caccggcccg agccgcgtcg agcacctcct cgaagggggg
    10261 catggggccc attatcggca ggcccggcgg gcggcgcaca tcgaggatca gcccactcgg
    10321 tggaccttca gcagattggt ccgggacctc cccaccggcg tcgaggcgac gaggacgacc
    10381 ggatcgccgc tcgacgcgag gccctcctcc cggaggcgtc ggtccatcat cgtcagcatc
    10441 gcgtccgtat cctcgggcgg atcgcacggc ctcgggtgca cgccccggaa caccgtcagg
    10501 cggcgaacca ccgcgtcgtc gtggctgtag gcgtagacgg ggacgggcag gcggacgccc
    10561 gagaggagac gggccgtgag gccggtgcgc gtgaagcacg ccacggcgac cgctcccgag
    10621 tccgcggcag cggtggcggc ggcgtcggcc accgcccacg cgtcgctccc ctccacgcgc
    10681 tcctccaccg tggcggagag gaagcgggct ccctcccgct cggcggtctc gaggatcgcc
    10741 gcggccgtgc gcgcggcctc gaccgggtgg cgtccgatcg cggtctcggc cgagagcagg
    10801 atcgcggcgg ccccgtcgaa ggccgcgccg gccacgtcac taacctccgc ccgcgtggga
    10861 cgccggctac ccgtcatcga ctcgagcatc tgcgtggcca cgaccgcggg gacccccgag
    10921 cggttggccc ggtacacgag gtccttctgg atcacgggga tgcgctcggg gggaagctcc
    10981 accccgagat cgcctcgggc caccatgacc acgtcggccg cctcgaggat cccgtcggac
    11041 tcctccaccg ccgagcgcgt ctcgatcttc gccacgagga gcgccggccc gtccccgagg
    11101 aggtccgcca acgcccgcac ctcggccgcg tggcggacga aagactgggc gacgaggtcg
    11161 atccccgcac tccccaccca agccaggtcc cgctcgtcct tctcggtgat cgcggggacg
    11221 ctcagccgtc ccgacgggac gttgaccccc gctctggagc ggatcagccc tccccgcact
    11281 accctcgtct ggacctcgtc gccggtcgtc tcctcgaccc gcagctccac gacgccatcc
    11341 gagagcagga cgcggtcgcc cacctcgaga tcgtccggga ggcccgcgtg gctcgtcggg
    11401 acgcccctcg cgtcgcccgg gcccttgccg ccgcggagca cgaacacgcc cccgtcctgg
    11461 agccggaccg cctccggctc gagctctccc agcctgacct tggggcccga caggtcggtc
    11521 atcaccgcga ccgcgcgccc cgcctcccgg gaggcctcgc gcaccgaccg cagaagccgc
    11581 tcgtgatcct cgcgggtccc gtgcgtcagg ttgatgcggg ccacgtccat gcccgcctcc
    11641 acgagctccg gcacgcggtc ggcgctcgcc gggccgagcg tgcagaccag cttcgtggcc
    11701 ctggtcatac ggagaacgcc cgcttcacgt cgtcgacgac cgaccggacc tcgcccgagc
    11761 cggccggtac ctcgggcggc tcctcggaga ggcgctccgg catgggcccg ccggcgtgct
    11821 cggcggccag ctgccgccac ccctcgggga ggtcgtcggg gagctcgacc ccggccatct
    11881 ccgcgaagta gatcgcccag gcccggggca ccacgcgcgc ccactgatac cccccgcctc
    11941 cggtggcgac ccagcgcccg ccggcgaccc cgtgcgccaa atcgtgcagc tcaaccgacg
    12001 cctcgcggta ggcccgggtc gagagcgcga ggtgcgccag cgggtcggta tggtgcgtgt
    12061 cgcagccgag ctgggtaacg agcacgtccg gatggaagcg ccgcaccagg ggcggcacga
    12121 tctcgcggaa ggcccggagc caggcctcgt cgctcgtcca gggcggcagc ggcacgttga
    12181 ccttcgagcc ctccgcgtcc ccctcacccc gctccgacac gaaccccgtc ccggggaaga
    12241 ggtagcggcc gctctcgtgg agcgagatcg tcagcaccct gggatcgtcg tagaagatcg
    12301 cctgcggccc gtcgccgtgg tgcacgtcca cgtccacgta cgcgatccgc tccacgccgc
    12361 ggtcgagcag ccaccggatg gccaccgccg ggtcgtcgta gacgcagaac ccgctggccc
    12421 gctcgggcat cgcgtggtgg aggccgcccg cggggttgaa ggcgtggtcc gcctctcccg
    12481 agagcacgag ccccgcggcc tcgatcgagg cgcccaccac ccgggccgac gcctcgtgca
    12541 tcgcggggaa gatcgggttg tcgccgggcc cgaacccgta ggcccaccag tccccgcgct
    12601 cccccgatcc cgcggccttc accacgtcga tgtaggcctg ggtgtgcacg aggccgacct
    12661 cctggtcgga ggccaggcgc gacgggacct cgtccacgtg gcccgcctcg aggacgccgt
    12721 aggcggcgat cagctcacgg gtcagcagga cccgcaccgg cttgaggggg tgctggggcc
    12781 cgtggtcgta ctcgacgacc tccggcgagt agacgagggc gacacgatcg ctcacgggcg
    12841 ccgaccggcc ctcagtcgat cccctgtgcc ttctggacct tccccgagta gtccaccgag
    12901 acggccttcc actccatgaa ctcgtcgagg gcctggggcc ccgcctcacg gtggccgttc
    12961 ccggtgttct tcatccctcc gaacggcagc tggatctcgg ccccgatcgt cggcgcgttc
    13021 acgtagacga tgccgaactc gagctgggtg accgcctcga agacgttcgt gatgtcgtgc
    13081 gtgtagatcg cggccgacag tccgtagtcc accgagttgg ccatccggat cgcctcgtcc
    13141 acgtcgtccg tgcggatgat ccccgtcacg ggcccgaaga tctcctcctg cgcgatccgc
    13201 atgtctggag tcatctcgtc gaacacggcc ggggcgatga agttcccctt ctcgtagccg
    13261 tcgccggtga gctcctcgcc cccggtaagc agcttggcgc cctccttctt cccgatctcg
    13321 atgtactcga ggatgcgctc cttctgcttc gagttgatca ccggagcgag ctcgacctct
    13381 ggatcgaggc cggacccgac cttcatcccc tccacccgct tcagcatccg ctcggtgaac
    13441 tcctcgcgcg cgtcgtcgtg gacgatgagc cgggaggtag ccgtgcagcg ctggccgctc
    13501 gtggcgaaag ccccccacga ggccgcctcg accgccaggt ccatctcggc gtcgggcatc
    13561 acgatcatca cgttcttgga cccgagctcg agggagcagc gcttcatcag cgcgccgcat
    13621 cgctcgttga tcttccgtcc cacctcgagc gagccggtga acgagatcgc gcggatgtcc
    13681 ggatgctcga ccatcggcgc gccggcctcc tccccgaagc cgtgcacca
//
LOCUS       DASUTZ010000122         4081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_550273, whole genome shotgun sequence.
ACCESSION   DASUTZ010000122 DASUTZ010000000
VERSION     DASUTZ010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550273"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VF097_05530"
     CDS             <1..293
                     /locus_tag="VF097_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX6262292.1"
                     /translation="LVFRREDGRPLDPDTVSQRFRRLVERSGLPRIRFHDLRHTFATL
                     SLKAGIPTEVVSRILGHKHVATTQAIYQHAVPALEEGAISRLAELLERAKSA"
     gene            complement(390..463)
                     /locus_tag="VF097_05535"
     tRNA            complement(390..463)
                     /locus_tag="VF097_05535"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(427..429),aa:Met,seq:cat)
     gene            complement(496..2007)
                     /locus_tag="VF097_05540"
     CDS             complement(496..2007)
                     /locus_tag="VF097_05540"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6262293.1"
                     /translation="MSVRGRVLIGTLCLVAAAVLLADFAVYRFLSSFLHGRVDDQLES
                     GMWAAPRILDLTGESSGPSPPILERGTPETVYPPGTYFAHLDEAGTMVRDFMITLESQ
                     GSLRDQPSSVPRLPSDLPGSGGSAGSSPSSRFIVESGDGTQYRVLADALAEGGTILVA
                     IPLTSTSATLQRLLLIEAGVTAAVLLAVGLLGFFVVRRGLRPLDRIADAAGAIAAGDL
                     SARVRPANDRTEVGRLGLALNTMLSQIETAFSERQASEDRLRRFVADASHELRTPLTS
                     IQGYAELFRRGASERPEDLAKVMRQIESESRRVSELVDELLLLARLDQADPSESPIEL
                     ARVDLTTIVAEAVEAARDLDPDRRIQLVANGPVIVMGDDVRLRQALDNLLSNARRYTP
                     TGTRVRVQVGIEDDTAAVDVADDGPGIDAEHAERVFERFYRVDRSRSRQKGGVGLGLS
                     IVAAIAEAHGGRATCEPVIPHGVRFRIELPLVERAEVVAEPAPGEGRATKAAR"
     gene            complement(2012..2743)
                     /locus_tag="VF097_05545"
     CDS             complement(2012..2743)
                     /locus_tag="VF097_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019044793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6262294.1"
                     /translation="MGERSAVASRGSEGVTPGLARVLVIDDDPGITDLVATALRYEGF
                     TVQVAATGREGLQAATSFRPHLLVLDVMLPDGDGFEIQQRLAAEGSEIPVLFLTARDT
                     TEEKVRGLLEGADDYVTKPFSLEELVARIHSVLRRNADLVSRNLVVGDLEMDEDTHEV
                     WRGGEPIELSPTEFNLLRFLMLNPRRVVSKTQILSHVWRYDFQGDASVVETYMSYLRK
                     KIDRGERKLLHTIRGVGYTLRPAGD"
     gene            2858..3157
                     /locus_tag="VF097_05550"
     CDS             2858..3157
                     /locus_tag="VF097_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262295.1"
                     /translation="MRIVLGIVVLALAACSSSPATEESVPGEVTGAITEIARGDGDEI
                     EAFTVDGTHRIRIDPGREYGFDLEHLVEHQTTGDPVRVTTESRYGVAYAVEILDA"
     gene            complement(3154..>4081)
                     /locus_tag="VF097_05555"
     CDS             complement(3154..>4081)
                     /locus_tag="VF097_05555"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEX6262296.1"
                     /translation="PLINAWGEIFPDLLTPLEEASPELREHFRYPEDIFLVQARQFSS
                     YHVTNPGQFYFGEDFWSLPIVAQGDPGIEGGPTNEPVQLEPYYVLLPLPGEEAQGERF
                     MLFIPFTPADRPNMVAWMAAVSDPDQYGRMVSFEFPLERAVPGPQNVAAFISQETEIA
                     REISLLDQLGSNVIYGDLLAIPVGRSFLYVQPLYLESAQAGSAIPELQRVVVVNGDTV
                     VMEPTLEEALARAFALEEEEPEQPPEPGEEPEEPQEPVPGDVAQLLAEAQGHFELAQA
                     ALERGDLGEYQRQIGLAEEAIAAAVEAAGAAP"
BASE COUNT          618 a         1447 c         1418 g          598 t
ORIGIN      
        1 agctggtctt ccggcgcgag gacggccggc ccctcgatcc cgacacggtg agccagcggt
       61 tccgccggct ggtggagcgg agcgggctcc cgcggatccg cttccacgat ctccgccaca
      121 cgttcgcgac gctgagcctg aaggcgggca tcccgaccga ggtggtgagc cggatcctgg
      181 gccacaagca cgtcgccacc acccaggcga tctaccagca cgcggtgccg gcgctcgagg
      241 agggagccat ctcgcgcctc gccgagctgc tggagagggc gaagagcgcg tagctcccgg
      301 tatcagactg gtatcagagg cggcgatccg cgcccaaacg aaaggggtgc cgatcgctcg
      361 gcacccctcc tgacctgcta attctctcgt tgcgcgggct ggatttgaac cagcgacctc
      421 tgggttatga gcccagcgag ctaccagact gctccaccgc gcaccccgat gctaccccag
      481 ggcttgcggg ggcgtctacc gggcggcctt cgtggcgcgt ccctcgccgg gcgcgggctc
      541 ggccaccacc tcggcccgct ccaccagcgg cagctcgatg cggaagcgca ccccgtgcgg
      601 gatgaccggc tcgcacgtag cccgcccccc gtgcgcctcg gcgatcgcgg ccacgatcga
      661 cagcccgagc cccacacccc ccttctgcct cgacctcgag cgatcgaccc tgtagaaacg
      721 ctcgaagacc cgctccgcgt gctcggcgtc gatccccggg ccgtcgtcgg ccacgtccac
      781 cgccgcggtg tcgtcctcga tccccacctg gacccggacg cgcgtgccgg tcggcgtgta
      841 gcgccgcgcg ttggagagga ggttgtccag agcctggcga agccggacgt cgtcccccat
      901 cacgatcacc ggcccgttcg ccacgagctg gatccgtcgg tccggatcga ggtcgcgcgc
      961 cgcctcgacc gcctcggcca cgatcgtggt gaggtccacg cgggcgagct cgatcgggct
     1021 ctccgaggga tccgcctggt ccagcctcgc cagcagcagc agctcgtcga cgagctcgct
     1081 cacccggcgg ctctcgctct cgatctggcg catcaccttg gccaggtcct cggggcgttc
     1141 ggaggcgcct cggcggaaga gctccgcgta gccctggatc gacgtcaggg gggtccggag
     1201 ctcgtgggag gcatcggcca cgaagcgccg gaggcgatcc tcggaggcct ggcgctcgct
     1261 gaaggcggtc tcgatctggg agagcatggt gttcagcgcc agcccgaggc gccccacctc
     1321 ggtcctgtcg ttcgccgggc ggacgcgggc ggagagatcg cccgcggcga tcgctccggc
     1381 cgcgtccgcg atccgatcca gcggccgcag ccccctccgg accacgaaga agcccagcag
     1441 cccgaccgcg aggagcacgg cggcggtcac gccggcctcg atcagcagca ggcgttggag
     1501 cgtggcgctc gtgctcgtca gggggatagc tacgaggatc gtgcctccct cggcgagggc
     1561 gtcggcgagc acccggtact gcgtgccgtc gccggactcc acgatgaatc gactggaggg
     1621 cgagctgccc gccgagcccc cggaaccggg gaggtccgac ggcagccggg gcacgctcga
     1681 aggctgatcg cgcagcgaac cctggctctc cagtgtgatc atgaagtcgc ggaccatcgt
     1741 cccagcctca tccaggtggg cgaagtaggt gccgggcgga tacacggtct ccggcgtccc
     1801 ccgctccagg atgggcggcg aggggcccga gctctcgccg gtcagatcga ggatccgggg
     1861 ggccgcccac atcccgctct cgagctgatc gtccacgcgg ccgtggagga acgacgaaag
     1921 gaaccggtag acggcgaagt ccgccagaag aaccgcggcg gccaccagac agagcgtccc
     1981 gatgaggacc cggccacgga cggacatcga ctcagtcgcc cgccggacgg agcgtgtagc
     2041 cgacaccacg aatggtgtgg agcagcttcc gctccccgcg gtcgatcttc ttgcggagat
     2101 agctcatgta ggtctccacg acgctcgcat cgccctggaa gtcgtagcgc cagacgtggc
     2161 tcaggatctg ggtcttggat accacgcgac gcggattgag catgaggaac cgcagcaggt
     2221 tgaactcggt cggcgagagc tcgatcggct cgcccccgcg ccagacctcg tgggtgtcct
     2281 cgtccatctc caggtcgccg acgacgaggt tgcgggagac gagatcggcg ttgcgtcgga
     2341 gcaccgagtg gatcctcgcc acgagctcct cgaggctgaa cggcttcgtg acgtagtcgt
     2401 cggctccctc gagcagcccc cgcaccttct cctcggtggt gtctctcgcg gtcaagaaca
     2461 ggaccgggat ctccgagccc tccgcggcga gtcgctgctg gatctcgaac ccgtccccgt
     2521 cggggagcat cacgtcgagg acgagaagat gggggcggaa cgaggtggcg gcctgcaggc
     2581 cctcccggcc cgtggccgcg acctgcacgg tgaatccctc gtagcggagc gccgtggcga
     2641 cgaggtcggt gatccccgga tcgtcgtcga tcacgaggac gcgggcgagt ccgggagtga
     2701 ctccttcgct cccccggctc gcgacggccg accgctcacc catcgctcac gctccttcca
     2761 cgagggttcc gctcccgggc gggggcgcgc gaagtatatc gagtcgggac cctcgtcgca
     2821 tcagggcccg cccgccgcac ccgctacagt ggcccagatg cggatcgtcc tggggatcgt
     2881 cgtcctggcg ctcgccgcct gttcctcctc ccctgcgacc gaggagtcgg tccccgggga
     2941 ggtcaccggc gcgatcaccg agatcgcacg gggggatggc gatgagatcg aggccttcac
     3001 cgtcgacggg acgcaccgga tccgcatcga tccgggtcgg gagtacgggt tcgacctgga
     3061 gcacctcgtg gagcatcaga cgaccgggga ccccgtgagg gtcacgaccg agtcgcggta
     3121 cggcgtcgcg tacgccgtgg agatcctcga cgcctagggc gccgcgccgg cggcctcgac
     3181 cgccgcggca atcgcctcct cggccagccc gatctgccgc tggtactcgc cgaggtcccc
     3241 gcgctccagc gccgcctggg ccagctcgaa gtgcccctga gcctcggcca gcagctgggc
     3301 cacgtccccg ggcacgggct cctgtggctc ctcaggctcc tcccccggct ccggtggctg
     3361 ctccggctcc tcctcctcga gcgcgaaggc cctggccagc gcctcctcca gcgtgggctc
     3421 catcaccacc gtgtcgccgt tgaccaccac gacgcgctga agctcgggga tggcggagcc
     3481 cgcctgcgcg gactcgaggt agaggggctg gacgtacagg aaggatcggc ccaccgggat
     3541 cgccaggagg tcgccgtaga tcacgttgga cccgagctgg tcgaggaggc tgatctcgcg
     3601 ggcgatctcg gtctcctggg agatgaacgc ggcgacgttc tggggtcctg ggacggcccg
     3661 ttccagcggg aactcgaagg acaccatgcg gccgtactga tccggatcgg agaccgcggc
     3721 catccaggcc accatgttcg ggcgatcggc gggcgtgaac gggatgaaga gcatgaaccg
     3781 ctccccctga gcctcctcgc cggggagcgg caggaggacg tagtagggct cgagttggac
     3841 cggctcgttc gtcggcccgc cctcgatgcc cgggtcgccc tgcgccacga tcggcagaga
     3901 ccagaagtcc tccccgaagt agaactgccc ggggttggtg acgtggtagc tggagaactg
     3961 cctggcctgc accaggaaga tgtcctccgg gtagcggaag tgctcccgga gctcgggtga
     4021 ggcttcctcc agcggcgtga ggaggtcggg gaagatctcc ccccacgcat tgatcagcgg
     4081 g
//
LOCUS       DASUTZ010000123         7070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1069227, whole genome shotgun sequence.
ACCESSION   DASUTZ010000123 DASUTZ010000000
VERSION     DASUTZ010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7070
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069227"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VF097_05560"
     CDS             complement(<1..299)
                     /locus_tag="VF097_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX6262297.1"
                     /translation="MSFLIVSNPEAGSAEEGMADRALHLLPDAEAFYLEPDADLGGAV
                     DEAVRDDRVVVAAGGDGTVSACVQHLVGRGTLGVLPAGTRNHLARDLDLNDEEAAL"
     gene            complement(309..1598)
                     /locus_tag="VF097_05565"
     CDS             complement(309..1598)
                     /locus_tag="VF097_05565"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6262298.1"
                     /translation="MTRLVVVGGGVAGLAAARAGARAGWRVTLLEASQRLGGKVLTEE
                     VDGVPWEWGPDAIAATKPRGEDLLEELGLLEDALPPATGRAYLLVGGELRPIPPGLTM
                     GFPKGPGALMEAARSGIVTRAEALRGAMEGAMPGRPRGSIAEVARARLGRGVAEKLTL
                     PLVSAVFGTADQDLEAAIPEMAGARSVTAGAARRPPVRFLGLRGGMGRLAEALVEKGV
                     EVRTGARAEAIEGSAGAYVVRADGAGVEADAVVVAVPAPSARPLLPSLPEGLGAYTSS
                     AVIHLRYPEGALGRPLDAAGYVAADPGVVQACSWVSSKWPHLEEPGPHLRAVVVEPAI
                     DDDEALALAVVEEVGRVMEAGADPDVVRVRPWALALPRYLPGHVETVRRIREALPPSI
                     QLAGASFDGVGVPDCVASGEEAFRRIAALWETTPDAG"
     gene            complement(1595..2164)
                     /locus_tag="VF097_05570"
     CDS             complement(1595..2164)
                     /locus_tag="VF097_05570"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014913626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX6262299.1"
                     /translation="MAEPTVACGGEVPQAADERKATYEQAPETQLQDGADYRAVLKTS
                     CGRIEIDLFEGQTPITVNNFVFLAREGFYDGTIFHRVIEGFMNQGGDPEGTGMGGPGY
                     EFEDEFVDDLTFDRPGLLAMANAGPGTNGSQFFITAAPTPHLDGAHTIFGEVTGGMDV
                     VNEINGLPTDPTDKPVDTVYIESIRIREA"
     gene            complement(2396..3790)
                     /locus_tag="VF097_05575"
     CDS             complement(2396..3790)
                     /locus_tag="VF097_05575"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HEX6262300.1"
                     /translation="MTRSSDLQAVAEQLGREPTTRFEVVARCPGGRHPLVIRNHPVAA
                     NGGPFPTLYWLTCPEAVAAVSRLESEGWIARFDERVEEDPECRERFEEAALDYAAERE
                     RLLPGAGALGGVGGSGGGVKCLHAHYAHHLAGGANPIGAWVAERVEPIHEETRGRVAA
                     VDMGTNSIRLLVADTKDGDVAEISRDLVITRLGQDVDRTKRLDPVALDRTIRVLERYA
                     RRARALRAYRIRVGATSAVRDASNRERLFAEVARVAGASAEVLTGEREAELSFLGATA
                     DAPAQPPYLVFDIGGGSTELAFGTERPEASVSLQLGSVRTHERVQPGDLPTAEDLAAM
                     DRVVEEALAEVRDAIPSGAATLVGVAGTTTTVQGIALGLDSYDPEALHGSVLSLEEAR
                     RVSEELASMTVAERAAIPVMTRGREDVIVAGSRILLGILRTFGFDACLVSERDILDGL
                     AIEMVRSRNGDPTR"
     gene            complement(3795..4199)
                     /locus_tag="VF097_05580"
     CDS             complement(3795..4199)
                     /locus_tag="VF097_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007956187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX6262301.1"
                     /translation="MGAFEPPFRKGAGPIADLEIGAVVTGTVQRITPYGAFIELEGGK
                     VGLVHISEIDRNYVRDVRDHLRETDEVEAKVVAIKDDGKIDLSIKALQDPPPKRLRRG
                     QDPDLEQKLKRFMRQSEERQVDVRKSTRDKRR"
     gene            complement(4443..4856)
                     /locus_tag="VF097_05585"
     CDS             complement(4443..4856)
                     /locus_tag="VF097_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262302.1"
                     /translation="MEPVHPEVLNGRNPWWREAWMKLSPSQRTAVWDAMKLGRRVPDD
                     DLLPFVYGLMAIERRTLRWIWPRFLWVEGITGVWIYFACFRIATPRWECWMFVALAFA
                     GVIALVLRLLMGRRRIRRAEASNLWGSASSEDPDV"
     gene            5124..6467
                     /locus_tag="VF097_05590"
     CDS             5124..6467
                     /locus_tag="VF097_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6262303.1"
                     /translation="MRPETRYAKSSGGYVAYQVFGDGPRDILFVPSWSQNLDAMWDEP
                     TLVRYFQRLSRAGRVICFDKRGSGVSDPVPLTNIPTLEEWMDDAVVAMDAAGSEQAAV
                     IGDAEGGPMAMLLAATVPDRVIALALVNTFARWSRAPDYPIGMPEGAFERLMDLYEQT
                     WGQNPDMLLLTAPSVADDPRIRDWFVRYQRLSMPPGAAAATYRWVSSLDVRAVLPTIS
                     APTLVLHRTENRHYRLAFGRYLAEHIPGARLVELPGADCYPFHVGDSEAVLDEIHAFL
                     TGVREGPPAERELATVLFTDIVGSTDLAAELGDARWLDLRDDHDAVLRRTIAAYRGQE
                     VKTTGDGFLATFDGPARAVQCAVRAGEAVRSLGLEIRAGLHTGEIERRDGDIGGLAVH
                     LAARVMGEAGAGDVLVSGTVRDLVVGSGIDFEDRGEHSLKGVPGTWRLLRVTGVP"
     gene            complement(6507..6749)
                     /locus_tag="VF097_05595"
     CDS             complement(6507..6749)
                     /locus_tag="VF097_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262304.1"
                     /translation="MVRRAWVAVLLGVLVAIPVAAVGWTWLITPDHVSEVRALEDRAI
                     VYIERFRAPELEFRGTYDRVPESDVRLATALRRVPA"
     gene            complement(6749..>7070)
                     /locus_tag="VF097_05600"
     CDS             complement(6749..>7070)
                     /locus_tag="VF097_05600"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEX6262305.1"
                     /translation="LDALIEGDVVTSVGGERATLRLDSDRLEGTTGCRRLTGRYVERG
                     DEIFATSLSAHGRCPDDLSGQDGHVVGVLGGGFTATVEGDRLTLTSMGGIGLIYRAAS
                     AGEE"
BASE COUNT          982 a         2679 c         2399 g         1010 t
ORIGIN      
        1 agcgcggcct cctcgtcgtt caggtcgagg tctcgggcca ggtggttgcg cgtgccggcc
       61 ggcagcacgc cgagcgtccc ccggccgacg aggtgctgga cgcaggcgct gacggtcccg
      121 tccccgcccg ccgccaccac gacgcgatcg tcccgcacgg cctcgtccac cgctccgccc
      181 aggtcggcgt ccggctccag atagaaggcc tcggcgtcgg ggaggaggtg gagggcgcga
      241 tcggccatgc cctcctcggc agacccggcc tccgggttgc tcacgatgag gaagctcacc
      301 gcccggtcct accccgcatc gggcgtggtc tcccacaggg cggcgatcct gcggaaggcc
      361 tcctccccgc tcgccacgca gtcggggacg cccacgccgt cgaaggaggc tcccgcgagc
      421 tggatgctgg gaggcagcgc ttcccggatc ctccggacgg tctccacgtg cccggggagg
      481 taccggggga gcgcgagggc ccacgggcgg acgcgcacca cgtccggatc ggccccggcc
      541 tccatcacgc ggcccacctc ctccacgacg gccaaggcca gggcctcgtc gtcgtcgatc
      601 gccggctcga cgaccacggc ccggaggtgc gggcccggct cctcgaggtg aggccacttc
      661 gacgagaccc aggagcaggc ctggaccacg cccggatccg cggcgacgta ccccgcggcg
      721 tcgagcggcc gacccagcgc cccctccggg tagcggaggt ggatcacggc cgacgaggtg
      781 taggccccga ggccttccgg gagcgagggg aggagggggc gcgccgacgg cgccggcacg
      841 gccaccacga cggcatcggc ctccacgccg gcgccgtcgg cgcgcaccac gtacgccccc
      901 gccgacccct cgatcgcctc cgcgcgggcc cccgtccgta cctcgacccc cttctcgacc
      961 agcgcctcgg ccagccgccc catcccgccc cgcagcccca ggaatcgcac cgggggccgt
     1021 cgcgcggccc cggccgtcac gctccgcgcc cccgccatct cggggatcgc ggcctccagg
     1081 tcctggtccg ccgtcccgaa gaccgccgag acgagcggca gggtgagctt ctcggccacg
     1141 ccccgtccca gcctggcccg tgccacctcc gcgatgctgc ccctcggacg gcccggcatc
     1201 gcgccctcca tcgcgccccg cagggcctcg gcccgcgtca cgatccccga ccgggccgcc
     1261 tccatcagcg cccccggccc cttcgggaac cccatggtca ggccgggcgg gatcggccgc
     1321 agctcgcctc cgaccagcag gtacgcgcgg ccggtggccg gcggcagggc gtcctcgagc
     1381 agcccaagct cctcgagaag gtcctcgccc cggggcttcg tggccgcgat cgcgtcgggc
     1441 ccccactccc acggcacccc gtcgacctcc tcggtgagga ccttgccccc caagcgctgg
     1501 gaggcctcca gcagggtgac gcgccatccg gcccgggctc cggcgcgggc cgcggcgagc
     1561 cccgcgaccc ctccgccgac caccaccagg cgcgtcaagc ttcgcggatc cggatcgact
     1621 cgatgtagac ggtgtcgacg ggcttgtcgg tgggatcggt ggggagcccg ttgatctcgt
     1681 tcaccacgtc catgccgccc gtgacctcgc cgaagatcgt gtgagccccg tcgaggtgcg
     1741 gcgtgggggc ggccgtgatg aagaactggc tcccgttcgt ccccgggcct gcgttggcca
     1801 tcgccagcag ccccggtcgg tcgaacgtca gatcgtccac gaactcgtcc tcgaactcgt
     1861 agcccgggcc ccccatcccc gtcccctcgg gatcgccgcc ctggttcatg aagccctcga
     1921 tcacacggtg gaagatcgtg ccgtcgtaga acccctctcg agcgaggaac acgaagttgt
     1981 tcacggtgat cggggtctgg ccctcgaaca ggtcgatctc gatccgcccg caggaggtct
     2041 tcagcaccgc ccggtagtcg gcgccgtcct ggagctgcgt ctcgggggcc tgctcgtagg
     2101 tcgccttcct ctcgtcggcg gcctggggga cctcgccccc gcaggccacg gtcggctcgg
     2161 ccatcgtctc ctcctgggtc ggggtcggcg tggggccggc cacgggctcc cccggcggct
     2221 cctccccacc gaacagcgcg agggctccca cggtgaggcc gccgagcgcg atcgcgcccc
     2281 cgacgacccc cgccgcgatc cgcctgcgac gcctccggcg gcgctcctcg gcgcgtcgct
     2341 cggcgagctt ctggagctgg cgctgcctcg atcgcttgct catcgggggg aaaggttagc
     2401 gggtggggtc gccgttccgc gagcgaacca tctcgatcgc cagcccgtcg aggatgtcgc
     2461 gctcggagac gaggcaggcg tcgaagccga aggtccggag gatgccgagg aggatgcggg
     2521 agccggccac gatcacgtcc tcgcggcccc gggtcatcac ggggatcgcg gcgcgctccg
     2581 ccacggtcat cgaggcgagc tcctccgaga cgcgcctcgc ctcctccagg gacagcacgc
     2641 tgccgtgcag cgcctcgggg tcgtacgagt cgagccccag ggcgatcccc tggacggtgg
     2701 tggtcgtccc ggccacgccc accagggtcg ccgcccccga tgggatcgcg tccctcactt
     2761 cggcaagcgc ctcctccacc acgcggtcca tcgccgccag gtcctcggcg gtcgggagat
     2821 ccccgggctg cacccgctcg tgggtgcgca cgctccccag ctggagcgag acggaggcct
     2881 ccggcctctc ggtcccgaag gcgagctcgg tgctgccgcc cccgatgtcg aagacgaggt
     2941 aggggggctg ggccggcgcg tcggccgtgg cccccaggaa ggagagctcg gcctcccgct
     3001 ccccggtgag gacctcggcc gacgcgccgg ccacccgcgc cacctccgcg aacagccgct
     3061 ccctgttcga ggcgtcccgc acggcgctcg tggcaccgac ccggatgcgg tacgcgcgga
     3121 gggctctcgc cctccgcgcg taccgctcca acaccctgat cgtccggtcg agggcgactg
     3181 gatccagccg cttcgtccgg tccacgtcct ggcccagcct cgtgatcacg aggtcccgcg
     3241 agatctccgc cacgtcaccg tccttcgtat cggccaccag cagacggatc gagttcgtcc
     3301 ccatgtccac cgcggccacg cgcccccgcg tctcctcgtg gatcggctcc acccgctccg
     3361 cgacccaggc cccgatcggg ttcgctcccc ccgccaggtg atgcgcgtag tgcgcgtgga
     3421 ggcacttcac gcccccgccg ctccccccga ccccgcccaa cgcccccgcg ccgggaagca
     3481 gccgctcccg ctccgccgcg tagtcgagcg ccgcctcctc gaaccgctcc cggcactcgg
     3541 ggtcctcctc cacccgttcg tcgaaccgcg cgatccatcc ctcgctctcc agacgcgaca
     3601 cggcagccac cgcctcgggg cacgtgagcc agtagagggt ggggaacggt ccgccgttcg
     3661 cggcgacggg gtggttgcgg atcacgagcg gatgccggcc gccgggacac cgcgcgacga
     3721 cctcgaaccg cgtcgtcggc tcgcgtccga gctgctccgc gacggcctgg aggtccgagc
     3781 ttcgggtcag ggcctcagcg ccgcttgtcc cgcgtggact tgcggacgtc cacctgacgc
     3841 tcctcgctct gccgcatgaa gcgcttcagc ttctgctcga ggtccgggtc ctggccccgg
     3901 cgcagccgct tcggcggagg gtcctggagc gccttgatcg agaggtcgat cttgccgtcg
     3961 tccttgatgg cgacgacctt ggcctcaacc tcatcggtct cacggaggtg gtcccggacg
     4021 tcgcggacgt agttgcggtc gatctcggag atgtgcacga ggccgacctt cccgccttcg
     4081 agctcgatga aggcgccgta cggggtgatg cgctggacgg tgccggtaac gacggcaccg
     4141 atctcgaggt ctgcgatcgg tcctgctccc ttccggaagg ggggctcgaa cgcccccatc
     4201 ggcctcgaac tatcccacgg cgcttccgaa cccgtcaacg cgccgccggg ttaggagata
     4261 agcgcctcca aggcggcacc ccccgatccg gtcgcgactt cggccatcgc ctcgtcggtc
     4321 ggctcggatc ccgcctcgcc ggtcagctcc gatcccggct cgccagtcgg atcgggtcgc
     4381 ggcgcagatt cgattcagat ccgggtgcgg cctcgctggc tcagggagcg aaggaagggc
     4441 actcaaacgt cgggatcttc tgaggaagca ctgccccaga gattggaggc ctccgcccta
     4501 cgaattcgac gtctgcccat caggagccgg agaacgagag cgatcactcc tgcgaacgcg
     4561 agggccacga acatccagca ctcccatcgg ggagtcgcga tgcggaaaca ggcgaagtag
     4621 atccacacac cggtgatccc ttccacccag aggaagcgcg gccagatcca ccgcagggtc
     4681 cggcgttcga tggccatgag cccgtacacg aacgggagga ggtcatcgtc agggaccctc
     4741 ctgcccagct tcatggcatc ccataccgcc gttcgctggc tcggggagag cttcatccag
     4801 gcttcacgcc accacgggtt gcgtccattg agaacctcgg ggtgcacggg ttccatgccc
     4861 gaagtatctc ttgtctggtt aagctgtggc cagaagcagc ctttgccgcg aaacgtcgcc
     4921 ccccaagccc cccgatccgg actccgactc cgactccgcc tcgccggtcg gctccggctc
     4981 gccggtcggc cccgactccg gccaaggctc ggcggcgatg tgaatgcttg agtcacccgg
     5041 cggctgaccg agatccgacg acccggcgtt ggaagcggcc actcggctcg tgtcagcatg
     5101 agggtgcccg ggaagggacc gggatgcgtc cggagacgag atacgcgaag tcgagcggag
     5161 ggtacgtcgc ctaccaggtg ttcggcgacg ggccgcggga catcctcttc gtcccgagct
     5221 ggagccagaa cctcgacgcg atgtgggacg agcccacgct cgtccgctac ttccagcggc
     5281 tgagccgcgc cgggcgggtg atctgtttcg acaagcgcgg ttcgggggtc tccgacccgg
     5341 tccccctgac gaacatcccc acgctggagg agtggatgga cgacgcggtc gtggccatgg
     5401 acgccgccgg gtccgagcag gcggcggtca tcggcgacgc ggagggtggg ccgatggcca
     5461 tgctgctggc cgccaccgtc cccgaccgcg tcatcgcgct cgcgctcgtg aacaccttcg
     5521 cccgctggtc acgcgccccg gactacccga tcggtatgcc cgagggcgcc ttcgagcgcc
     5581 tcatggacct ctacgagcag acctgggggc agaacccgga catgctcctg ctcaccgcgc
     5641 cgagcgtcgc cgacgaccct cgcatccggg actggttcgt ccggtaccag cggctgtcaa
     5701 tgcccccagg agctgcggcg gccacgtacc gctgggtgtc ctcgctcgac gtgcgggcgg
     5761 tcctccccac catctcggcc cccaccctcg tcctgcaccg gacggagaac cgccactatc
     5821 ggctcgcctt cggcagatac ctggccgagc acatcccagg ggcgcgactc gtggagctgc
     5881 cgggcgccga ctgctacccg ttccacgtcg gagacagcga ggcggtcctc gacgagatcc
     5941 acgcgttcct cacgggggtt cgggagggtc cgcccgccga gcgggagctg gccacggtcc
     6001 tgttcaccga catcgtgggc tcgaccgacc tggccgcgga gctcggtgac gcccggtggc
     6061 tcgacctccg cgacgatcac gacgcggttc tccggcggac catcgcggcc taccgagggc
     6121 aggaggtcaa gacgacgggc gacggcttcc tcgcgacctt cgacggcccc gcgcgagccg
     6181 tacagtgcgc agttcgagcc ggtgaagccg ttcgctccct ggggctcgag atcagggcgg
     6241 ggctgcacac gggcgagatc gagcgtcggg atggggacat cggggggctc gcggtccacc
     6301 tcgcggcccg ggtgatgggg gaggcaggcg cgggcgacgt ccttgtctcg gggacggtgc
     6361 gcgacctcgt ggtggggtcg gggatcgact tcgaagaccg aggcgagcac agcctgaagg
     6421 gggtcccggg cacgtggcgg ctgcttcgcg tcacgggtgt tccctgacca tcgagtgggg
     6481 gtccatcagg agggactctg agaggctcag gctggaacgc gcctgagcgc tgtggccagc
     6541 cggacgtcgc tctcggggac gcgatcgtag gtccctcgga actcaagctc gggagcccgg
     6601 aatctctcga tgtagacgat ggcgcgatcc tccagagccc tgacctccga gacgtggtct
     6661 ggggtgatca gccaggtcca ccccaccgcg gctacgggga tcgccacgag gacccccagg
     6721 aggaccgcca cccaggccct ccgcaccatc actcctcacc tgccgaggcg gcgcggtaga
     6781 tgaggccgat ccctcccatc gaggtgaggg tgagccgatc accctcgacg gtcgcggtga
     6841 accctccgcc cagcacgccg accacgtgcc cgtcctggcc cgacagatcg tcggggcagc
     6901 gcccgtgcgc cgacaggctg gtggcgaaaa tctcgtcgcc tcgctcgacg taccggccgg
     6961 tgagccggcg gcagccggtc gtgccctcga gacggtcgga gtccagccgc agggtcgccc
     7021 gctcacctcc gacggaggtg acgacgtcgc cttcgatcag cgcatcgagg
//
LOCUS       DASUTZ010000124         3758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_551664, whole genome shotgun sequence.
ACCESSION   DASUTZ010000124 DASUTZ010000000
VERSION     DASUTZ010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551664"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..843
                     /locus_tag="VF097_05605"
     CDS             187..843
                     /locus_tag="VF097_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX6262306.1"
                     /translation="MGTCPGCSASNQPGSSFCWQCYAPLSERAERAATLEPIARTPER
                     RWRFSIPGPGQIIGHTLVGLFVLSVIGSIWLVLERRQVTLPDTVAGFERIENPDFDAY
                     AREVEEELGIAVSAGTYLRGRRFFGVMVGSELDDARPADRIRELPAIQDGVAFQKAKI
                     RSFQSPTAFFACVPFVGLANGTLCLWNGSGTSGLLYAEALGMRSARELARQVQVAVGS
                     "
     gene            complement(850..3513)
                     /locus_tag="VF097_05610"
     CDS             complement(850..3513)
                     /locus_tag="VF097_05610"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX6262307.1"
                     /translation="MSERCVLAYSGGLDTSVAARSLREERGLDVVAAFVDLGQPYDLG
                     EVKARAGAAEADLRVIDARDRFAEEFCLPALHANALYEGTYPLVSSLARPLIAHEVVA
                     LAREVGARFVAHGCTGKGNDQVRFETSFAALAPELEVLAPIREASRSREESLGLAARW
                     GIPQAREASTYSVDENLWGRTVEAGPLEDPWLEPPRDAFELTVDPLQAPDAPAEVTIA
                     LEAGRPVALDGARLPIAELVARLNGIAGAHGFGRVDMIENRLVGIKSRELYEVPGALA
                     LITAHRDLEDLTLERDLAHEKAGLERRWAELCYYGQWYGPLHEGLRSFMAAAQEDVTG
                     EVRLRFFKGSCAVIGRRSPRALYDLALATYEGRADRFDQGHAEGFIRLWSLPVRTWAG
                     RRGDPPPASRPEPVRPGSVTPHVEEDPASAGPSAVREPPTERSPAPLWGGRFGEASAA
                     DAVAFTRSQVDRRLVPQDLRATRAHCEALARAGLLEPGERDRLVAEVDRLLDEAERGS
                     FPFRDEDEDVHLAVERVLTERLGDLGARVHAGRSRNDLVVTDLRLWVGQAATGLAERA
                     RGLARALADRAEEHLETPLPAYTHLQRAQPSTLAQHLLAHAFPLVRDAERLERAGAGA
                     GASALGAAAAAGTTLPLDRDVAAAALGLSRSFDNSIDAVSDRDFALDLLSACLSTALH
                     LSRLGEDLVLWCSREFGFARPADAYATGSSMMPQKRNPDVAELARAQAGRILGDLVHL
                     ATVLKGLPLSYDRDLQEDKAAVFDAHDALVPALEAMAGMVRTLSFDVDRMAEAAGDPD
                     LSATDVAEHLVARGVPFREAHGVVGRLVARLEEEGRSLRALRPEEWAEVHPALEDPPH
                     LSGGAAARPSPGGPSPASTREQLARLRAALR"
     gene            complement(3510..>3758)
                     /locus_tag="VF097_05615"
     CDS             complement(3510..>3758)
                     /locus_tag="VF097_05615"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEX6262308.1"
                     /translation="EEQLRHVLQEFALAMVASGNLVVVRTPPGAANAVAQVLDRVEVP
                     DVIGTVAGDDTILVVAREGVRGRTLQRRLEALARREAG"
BASE COUNT          512 a         1405 c         1371 g          470 t
ORIGIN      
        1 gcgccacctc aagctcgggg acgagttcga ggtggagggg atgaaggtcc ccgcgcccag
       61 ggcggtcttc acctcgagcc gtcgagggcg cggacgggga cgggtgcggc cccgacggcg
      121 gtaacgaacc ggacgttccc ctcccaacgc tcaaggtggg ggaagggacg ggccgatgga
      181 ggtcccatgg ggacctgtcc cggctgctcg gcgtcgaacc aacccggctc atccttctgc
      241 tggcagtgct acgcgccgct ctcggagaga gccgagcgag cggccacgct cgagcccatc
      301 gctcgaactc ccgagaggcg atggcgcttc tcgatcccgg gaccggggca gatcatcggc
      361 cacacgctgg tcggcctctt cgtgctgtcg gtgatcggga gcatctggct ggtcctcgag
      421 cggcgccagg tgaccctccc cgacacggtc gccggcttcg agcggatcga gaacccagac
      481 ttcgacgcct acgcccgcga agtggaggag gagttgggga tcgccgtcag cgcggggacc
      541 tacctgcggg ggaggcggtt cttcggcgtg atggtcgggt ccgagctcga cgatgcccgg
      601 cccgccgaca ggatccgcga gctccccgcc atccaggacg gagtggcctt ccagaaggcc
      661 aagatccgct cgttccagtc gcccaccgcg ttcttcgcct gcgtgccgtt cgtcggcctg
      721 gccaacggga ccctctgcct gtggaacgga tcgggcacct ccggcctcct ctacgcggag
      781 gccctcggca tgaggtccgc tcgcgagctc gcccggcagg tccaggtggc cgtgggctcc
      841 tgaggcgagc taccggaggg ccgcccgcag ccgcgcgagc tgctcgcggg tcgaggcggg
      901 ggacggtccg cccgggctgg ggcgcgccgc tgccccaccg ctgagatgtg gtggatcctc
      961 gagcgccggg tggacctcgg cccactcctc cggccggagg gcgcgcagcg agcgcccctc
     1021 ctcctccagc cgcgccacga ggcgccccac cacgccgtgc gcctcccgga agggcacccc
     1081 cctcgccacg aggtgctcgg ccacatcggt ggccgacagg tccgggtccc ccgccgcctc
     1141 ggccatccga tccacgtcga aggacagggt gcggaccatc ccggccatcg cctccagcgc
     1201 gggcacgagg gcgtcgtggg cgtcgaacac ggcggccttg tcctcctgga ggtcgcggtc
     1261 gtaggagagc gggagcccct tgaggactgt cgccaggtgc acgaggtccc ccaggatgcg
     1321 accggcctga gcgcgggcta gctcggcgac gtccgggttc cgcttctgcg gcatcatgct
     1381 cgaccccgtg gcgtaggcgt cggcggggcg ggcgaacccg aactcccgcg agcaccagag
     1441 cacgaggtcc tcccccagcc tcgagagatg gagagctgtg gagaggcacg ccgagagcag
     1501 gtcgagggcg aagtcccggt ccgacaccgc gtcgatcgag ttgtcgaacg agcgggagag
     1561 ccccagggcg gcggccgcga cgtcccggtc cagggggagg gtggtgcccg cggcggcggc
     1621 ggcgccgagc gcggacgcgc cggcgccggc gccggcccgc tccagccgct ctgcgtcccg
     1681 gaccagcggg aacgcgtggg ccaggaggtg ctgggcgagc gtggagggct gggcccgctg
     1741 gaggtgcgtg tacgcgggca gaggggtctc gaggtgctcc tcggctcggt cggcgagcgc
     1801 ccgggccagc ccgcgggccc tctcggccag cccggtcgcc gcctgcccga cccacagccg
     1861 aaggtcggtc accacgagat cgttgcggct gcgcccggcg tgcacgcggg cccccaggtc
     1921 tcccagccgc tcggtcagca cccgctcgac cgccaggtgc acgtcctcgt cctcatcccg
     1981 gaaggggaac gagccgcgct ccgcctcgtc cagcaagcgg tcgacctcgg ccaccagccg
     2041 gtctcgctcc cccggctcca ggagcccggc ccgggccagc gcctcgcagt gcgcgcgcgt
     2101 ggcgcggagg tcctgcggca cgagccggcg atcgacctgg cttcgggtga acgccacggc
     2161 gtccgccgcc gaagcctccc cgaaccggcc cccccagagg ggcgcggggg accgctcggt
     2221 cggaggctcc cggacggcgg acgggcccgc ggaggccggg tcctcctcca cgtgtggggt
     2281 cacgctcccc gggcggacag gctcgggccg cgaggccggc ggcggatcac cccgccgtcc
     2341 cgcccacgtc cgcacgggca gtgaccacag cctgatgaag ccctccgcgt gaccctggtc
     2401 gaagcgatcg gcccggccct cgtaggtggc gagcgcgaga tcgtagaggg cccgagggct
     2461 ccgccggccg atgacggcgc agctcccctt gaagaaccgg agccggacct cgccggtcac
     2521 gtcctcctgc gcggcggcca tgaacgaacg gagaccctcg tggagcgggc cgtaccactg
     2581 gccgtagtag cagagctccg cccatcgccg ctcgagcccg gccttctcgt gggccaggtc
     2641 gcgctcgagc gtcaggtcct caaggtcgcg atgggccgtg atcagcgcca gcgcgccggg
     2701 gacctcgtac agctcgcgag acttgatgcc gaccagacgg ttctcgatca tgtccacccg
     2761 cccgaacccg tgcgccccgg cgatcccgtt gagcctcgcc acgagctcgg cgatcggcag
     2821 ccgtgccccg tccagcgcga ccgggcgccc ggcctccaac gcgatcgtca cctcggccgg
     2881 ggcatcggga gcctgcagcg ggtcgaccgt cagctcgaag gcgtcccggg gaggctccag
     2941 ccagggatcc tcgagcgggc cggcctccac cgtccgcccc cacaggttct cgtccaccga
     3001 gtaggtggag gcctcgcgag cctgggggat gccccagcga gcggcgagcc ccaaggactc
     3061 ctctcgggac cgtgacgctt ccctgatggg ggccaggacc tccagctccg gggcgagcgc
     3121 ggcgaacgag gtctcgaagc ggacttggtc gttccccttg cccgtgcagc cgtgggcgac
     3181 gaaccgggcg ccgacctcgc gcgccaaggc cacgacctcg tgcgcgatca ggggccgagc
     3241 cagcgaggag acgagcgggt acgtcccctc gtagagcgcg ttggcgtgga gcgcggggag
     3301 gcagaactcc tccgcgaagc ggtcgcgggc gtcgatcacg cgcaggtcgg cctcggccgc
     3361 ccccgccctg gccttcacct cgccgaggtc gtaaggctgg cccaggtcca cgaacgcagc
     3421 gaccacatcg agcccccgct cctcccggag cgagcgggcg gcgaccgagg tgtcgaggcc
     3481 ccccgagtag gccaggacgc agcgctcgct catcccgcct cccgccgggc gagggcctcg
     3541 aggcgccgct ggagcgtgcg cccacgaacc ccctcgcggg ccacgacgag gatcgtgtcg
     3601 tccccggcca cggttccgat cacgtcgggc acctcgaccc ggtcgagcac ctgggccacg
     3661 gcgttcgccg cccccggcgg cgtacggacg acgacgaggt tgcccgaggc caccatggcg
     3721 agcgcgaact cctggaggac gtgccggagc tgctcctc
//
LOCUS       DASUTZ010000125         5949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_940721, whole genome shotgun sequence.
ACCESSION   DASUTZ010000125 DASUTZ010000000
VERSION     DASUTZ010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5949
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940721"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..95)
                     /locus_tag="VF097_05620"
     CDS             complement(<1..95)
                     /locus_tag="VF097_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016829522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX6262309.1"
                     /translation="MAEPVTMPQLGETVVEGTIVKWLKSEGDSVDR"
     gene            complement(169..1557)
                     /gene="lpdA"
                     /locus_tag="VF097_05625"
     CDS             complement(169..1557)
                     /gene="lpdA"
                     /locus_tag="VF097_05625"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014912445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX6262310.1"
                     /translation="MADADVIVIGAGTGGYSCALRGAQLDKKVVLVERDDRVGGTCLL
                     RGCIPTKAILESAATMDRINRAEEWGIKASGEPDWAGVRAFEDGIVDKLVSGVTGLLK
                     ARKVEVVQGTAKLTAPGKVEVDTADGKRSLEAPDVVVATGSQPKLLPGMQLGKRVITS
                     DQALFADSLPKSVVVIGAGAVGLEFASLYRSFGAEVTLIEALPRLAPLEDEDISKEVD
                     RAFRKRGIATHAGAKVQDVKETQKQVTVTFETAKSGTETVKAEICLVAVGRGPISDGV
                     GLKDVGVEMERGYVKVDGQLRTNVNGVWAIGDVAATPYQLAHVSMAEGVAVAERIAGM
                     DVPELDYAGVPRVTFSSPEIASVGLTEEQAKERGHEVETDNLSFRGIGKANIVGEGGT
                     VKVVAETDGPVLGVHMIGPHVTDLVAEAMLITNWEAIPAEVAALIHPHPTLSEGIGEA
                     HLALAGKPLHTM"
     gene            1658..2734
                     /gene="gcvT"
                     /locus_tag="VF097_05630"
     CDS             1658..2734
                     /gene="gcvT"
                     /locus_tag="VF097_05630"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014441509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX6262311.1"
                     /translation="MDRQLQHTPLEEEHRALGAALGPFGGWLMPIEYEGVLKEHAAVR
                     ERVGVFDLTHLGKVRVRGPRAPQHLQRILTNDLSKVAVGQAQYTLVLTEKGGIVDDLI
                     AYRTGEEEWLVVPNAGNTARVLGILEDGDADVRHRPDVVTLAVQGPEAIGLVAPSFPE
                     AEGLSFMEVAASSFEGTEVLVARSGYTGEPGVELFVSEDAAPALWRRLLERGATPVGL
                     GARDTLRLEMGYPLHGNDISEDRTPLEAGLSWAVAMDKGGFTGREALARIKEEGGPTE
                     RLRGLRMLGRRIPRPHFPVFKDGRPVGETTSGTFSPTLRVGIAMAYLDRTIEPGDTVQ
                     VDVRGKAAEAGVVRPPFLDRSPKA"
     gene            complement(2731..3408)
                     /locus_tag="VF097_05635"
     CDS             complement(2731..3408)
                     /locus_tag="VF097_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262312.1"
                     /translation="MLRRISLLFLLAAAVAASITGGPAFSPPAEARPVREVAAGRVSA
                     LVPEKWDFRPMRSEVRAGEGIQASGDLRRWGSLERREIGLEAYWIDAAKIGLPSDYYY
                     LAAEGPAMTRLPVGVGCRRDRLEILSDHRPAFDRRRFSPGDYVATARGTCVTRRGLTR
                     WASFVAAPGYGPVRGIGIPESGLYFAFVMVPDGPRADRRADRLLSAVAFGGTEVAEFI
                     DTARGQV"
     gene            complement(3464..4090)
                     /locus_tag="VF097_05640"
     CDS             complement(3464..4090)
                     /locus_tag="VF097_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6262313.1"
                     /translation="MRVLIVDDHPVTRDGLRAALGTDREVEVIGEATTGDEAIARVND
                     LDPDVVFMDVRMPGMNGIEATKVIRRKHPDTKIILFTVDESRASISEAIQAGVNGYLL
                     KDVGAEELINAARLALDGKAVIHPALTQAFIEEVRLVNRPQESPLSRREVEILQKVAY
                     GATTKEVATQLGISPHTVKTHLERIFEKLGANDRAQAVAIAIRQGLVQ"
     gene            4191..4538
                     /locus_tag="VF097_05645"
     CDS             4191..4538
                     /locus_tag="VF097_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEX6262314.1"
                     /translation="MRDETATEAAANGSSVEGIEEQVLESLKNVIDPELGINIVDLGL
                     VYEVSVDDGTLHVEYTLTTMGCPIGPLIESQMRQTISGIEGVEDFEAEMVFSPPWSPD
                     KMSEEAKAALGMI"
     gene            4621..>5949
                     /gene="sufB"
                     /locus_tag="VF097_05650"
     CDS             4621..>5949
                     /gene="sufB"
                     /locus_tag="VF097_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX6262315.1"
                     /translation="MAISQEEHLKELSKGYRFDWKDPSHSVFEPKKGLSEAVVEEISH
                     LKSEPDWMRKYRLKALKHFQGRPMPWWGADLSDIDFDDIYYFIRSTEKQAQSWEDLPE
                     DIRGTWDKLGIPEAEKKFLGGVSAQYESEVVYHKIKDELDKLGVIFTDMDTALREHPD
                     IVKEYFGTIIPANDNKFAALNSAVWSGGSFIYVPEGVHVEIPLQAYFRINAESMGQFE
                     RTLIIAEPGSYVHYVEGCSAPIYTTNSLHSAVVEIVAKPNSRVRYTTIQNWSNNVYNL
                     VTKRAAAYEDAVMEWIDGNIGSKVTMKYPSIYLLGKGARGEVLSVAYAGKGMHTDAGG
                     KAIHAAPYTTSVINSKSVSKDGGRTGYRGLVRVEEGAHHAKSTVRCDALILDDESASD
                     TFPYIEVEDDTAALAHEATVSKVGEDQLFYLMSRGLSEQEATAMVVNGFIE"
BASE COUNT          951 a         2058 c         2032 g          908 t
ORIGIN      
        1 cggtcgacgc tgtccccctc gctcttcagc cacttgacga tcgtcccctc gacgacggtc
       61 tcgcccagct gcggcatcgt caccggctcg gccacgtctc ccgacctcct cgtgtcgtcc
      121 tcgcgttgtt gtaccaccgc gtcccggcgc ccaaccgggg cgcgcccttc acatcgtgtg
      181 cagcggcttg cctgccagcg ccaggtgcgc ctccccgatg ccctccgaga gcgtggggtg
      241 ggggtggatc agggcggcca cctcggcggg aatcgcctcc cagttcgtga tgagcatcgc
      301 ctcggccacg aggtcggtca cgtgcgggcc gatcatgtgc acgccgagga ccgggccgtc
      361 cgtctcggcc acgaccttca ccgtccctcc ctcgcccacg atgttggcct tgccgatgcc
      421 ccggaaggag aggttgtcgg tctcgacctc gtgcccgcgc tccttggcct gctcctcggt
      481 gaggcccacc gacgcgatct ccgggctgga gaaggtcacg cggggcacgc ccgcgtagtc
      541 gagctcgggc acgtccatcc ccgcgatccg ctccgcgacc gcgaccccct cggccatcga
      601 gacgtgggcc agctggtagg gggtggccgc cacgtcgccg atcgcccaga cgccgttcac
      661 gttcgtgcgc agctgcccat cgaccttgac gtagccccgc tccatctcga cgccgacgtc
      721 cttgaggccc acgccgtccg agatcgggcc gcgcccaacc gcgacgaggc agatctcggc
      781 ctttacggtc tcggtcccgc tcttcgcagt ctcgaacgtc actgtgacct gcttctgcgt
      841 ctccttgacg tcctggacct tcgccccggc gtgcgtcgcg atgccccgct tccggaacgc
      901 gcggtccacc tccttggaga tgtcctcgtc ctcgagcggc gcgagccggg gcagagcctc
      961 gatcagcgtg acctcggccc cgaacgacct gtagagggag gcgaactcga ggccgaccgc
     1021 gccggccccg atcacgacta ccgacttggg gagggagtcc gcgaacagcg cctggtcgct
     1081 ggtgatcacg cgcttgccga gctgcatccc ggggagcagc ttgggctggg agccggtagc
     1141 gaccacgacg tcgggcgcct cgagcgaccg cttgccgtca gcggtgtcga cctcgacctt
     1201 gccgggagcg gtgagcttcg ccgtgccctg cacgacctcc acctttcggg ctttcagcag
     1261 cccggtgacg ccgctcacga gcttgtcgac gatcccgtct tcgaacgccc ggacgccggc
     1321 ccagtcgggc tcgccggacg ccttgatgcc ccattcctcg gcccggttga tcctgtccat
     1381 cgtggccgcc gactcgagga tcgccttcgt ggggatgcag ccgcggagga gacaggtccc
     1441 tccgacccgg tcgtcccgct ccacaagcac gaccttcttg tcgagctggg cgccccggag
     1501 ggcgcacgag tagccgccgg tgcctgcgcc gatcacgatg acgtctgcgt cggccatgcc
     1561 aagatccttt cctcgttggc ctgggcccgg ggcggggccc gctgcgggcc gcaaccgtca
     1621 tactaccgtt cggcccgtcc acgcaccgag gagccccatg gatcgacagc tgcagcacac
     1681 gccgctggag gaggagcacc gagcgctggg ggccgcgctg gggcccttcg gcggctggct
     1741 gatgccgatc gagtacgagg gcgtcctgaa ggagcacgca gccgtgcggg agcgggtggg
     1801 ggtcttcgac ctcacgcatc tcgggaaggt ccgggtgcga ggacctcgcg ccccccagca
     1861 cctccagagg attctcacga acgacctgtc gaaggttgcg gtggggcagg ctcagtacac
     1921 gctcgtcctc accgagaagg gtgggatcgt cgacgacctc atcgcctacc ggaccggcga
     1981 ggaggagtgg ctcgtcgtcc ccaacgcggg gaacacggcg cgggtcctgg ggatcctcga
     2041 ggacggcgac gccgatgtgc ggcaccgccc cgacgtcgtg acgctggcgg tccagggccc
     2101 cgaggcgatc gggctcgtgg cgccttcatt cccggaggcg gagggcctct cgttcatgga
     2161 ggtggccgcc tcatcgttcg aggggaccga ggtgctcgtg gcgaggtcgg gctacacggg
     2221 cgagcccggc gtcgagctgt tcgtctccga ggacgcggcg ccggccctct ggcgccggct
     2281 gctggagcgg ggtgcgacgc cggtgggcct cggggcccgg gacaccctac gcctggagat
     2341 gggctacccc ctccacggca acgacatctc cgaggatcga accccgctgg aggccgggct
     2401 ctcctgggcg gtggcgatgg acaagggcgg cttcacgggc cgcgaggcgc tggcgcggat
     2461 caaggaggag ggcgggccga ccgagcgcct ccggggtctc cggatgctcg gccggcgcat
     2521 cccgcgtccc cacttccccg tattcaagga tggccgtccg gtgggcgaga ccacgagcgg
     2581 cacgttctcg cccaccctgc gggtggggat cgccatggcc tatctcgatc gcacgataga
     2641 gccgggcgac acggtgcagg tcgacgtccg cgggaaggct gccgaggccg gggtcgtccg
     2701 cccgcccttc ttggaccgca gcccgaaggc ctagacctgc ccccgggccg tgtcgatgaa
     2761 ctccgccacc tcggtcccgc cgaaggcaac ggccgaaagg aggcggtccg cgcgccggtc
     2821 ggcccgcggc ccgtcgggga ccatgacgaa ggcgaagtag aggccggatt cggggatccc
     2881 gatcccgcgc accggtccgt agccgggtgc ggcgacgaac gaggcccacc gggtgaggcc
     2941 acgccgtgtg acgcacgtcc cacgggccgt ggccacgtag tcgcccggcg agaagcgcct
     3001 ccgatcgaag gccggtcgat ggtcggagag gatctcgagc cggtcccgcc tgcagcccac
     3061 gccgacgggg agcctggtca tcgcggggcc ttcggcggcc aggtagtagt agtcggaggg
     3121 cagcccgatc ttggccgcgt cgatccagta ggcctcgagc ccgatctcgc gccgctccag
     3181 agatccccac cgccgcaggt cccccgacgc ctggattccc tcgcccgcac ggacctccga
     3241 acgcatcggc cggaagtccc acttctcggg gacgagggcg gagacgcgcc cggccgccac
     3301 ctctcggacg ggacgagcct cggccggggg gctgaaggcg ggcccgcccg tgatgctcgc
     3361 cgcgacggca gcggcgagca ggaacagcaa cgagatccgg cgcagcatcc gaggatcccc
     3421 ccttgggcag agtgtagggg agggatggac ccgcggctag accctactga acgaggccct
     3481 gtcggatggc gatcgccacg gcctgcgccc ggtcgttcgc cccgagcttc tcgaagatgc
     3541 gctcgaggtg ggtcttgacg gtgtgcgggg agatcccgag ctgagtggcg acctccttgg
     3601 tggtggcccc gtaggccacc ttctgaagga tctcgacctc ccgacgggag agcggcgact
     3661 cctgcgggcg gttgaccagc cggacctctt cgatgaacgc ctgcgtgagc gcggggtgga
     3721 tcaccgcctt gccgtccaag gccagcctgg ccgcgttgat gagctcctcg gctcccacgt
     3781 ccttcaggag gtagccgttg acgcccgcct ggatcgcctc ggagatcgag gcccgggact
     3841 cgtccactgt gaagaggatg atcttcgtgt cgggatgctt ccgacggatg accttcgtgg
     3901 cctcgatgcc gttcatcccc ggcatccgca cgtccatgaa gaccacgtcg gggtcgagat
     3961 cgttcacccg agcgatcgcc tcgtccccgg tggtcgcctc cccgatgacc tccacctcac
     4021 gatccgtccc gagcgcggcc cggagcccat cacgcgtcac ggggtgatcg tcgacgatca
     4081 gcactcgcat gccatccgtt cccttctgcc ggaaacgcga agaacctccc ggggcgggac
     4141 acgcgccgac cccgtgggta ggatagaccc gagttcatat ggaagaaagc atgcgggacg
     4201 agacggcgac cgaggccgcg gcgaacgggt cctccgtgga ggggatcgag gagcaggtcc
     4261 tcgagtccct gaagaacgtg atcgaccccg agctcgggat caacatcgtg gacctcgggc
     4321 tcgtctacga ggtgtcggtc gacgacggca ccctccacgt cgagtacacg ctcaccacta
     4381 tgggttgccc catcgggccc ctgatcgagt cgcagatgcg ccagaccatc tccgggatcg
     4441 agggcgtcga ggacttcgag gccgagatgg tgttctcccc gccctggtcc cccgacaaga
     4501 tgtcggagga ggccaaggcg gcgctgggga tgatctgacc cggcgaattc cgactttcca
     4561 ggtagggatt cgggtagtag actggagtca gtccagcgca tcgaagggtg accagaacac
     4621 atggcgatct ctcaggaaga gcacctcaag gagctgagca aggggtaccg gttcgactgg
     4681 aaggacccct cccactcagt cttcgagccc aagaaggggc tctcggaggc cgtggtcgag
     4741 gagatctccc acctcaagtc ggagccggac tggatgcgga agtaccggct gaaggccctg
     4801 aagcacttcc agggccggcc catgccgtgg tggggcgcgg acctgtcgga catcgacttc
     4861 gacgacatct actacttcat ccggtccacc gagaagcagg cccagagctg ggaggacctg
     4921 cccgaggaca tccgagggac ctgggacaag ctggggatcc ccgaggccga gaagaagttc
     4981 ctcggcgggg tgagcgcgca gtacgagagc gaggtcgtct accacaagat caaggacgag
     5041 ctcgacaagc tgggcgtgat cttcaccgac atggacacgg cgctccgtga gcatcccgac
     5101 atcgtcaagg agtacttcgg gacgatcatc ccggcgaacg acaacaagtt cgccgcgctc
     5161 aactccgcgg tgtggtcggg cgggtccttc atctacgtcc ccgagggcgt ccacgtcgag
     5221 atcccgctgc aggcctactt caggatcaac gccgagagca tggggcagtt cgagcggacg
     5281 ctgatcatcg ccgagcccgg ctcctacgtg cactacgtcg agggctgctc ggccccgatc
     5341 tacaccacga actcgctcca ctccgcggtg gtcgagatcg tcgccaagcc gaacagccgg
     5401 gtccggtaca cgacgatcca gaactggtcc aacaacgtct acaacctcgt gacgaagagg
     5461 gccgcggcct acgaggacgc ggtgatggag tggatcgacg gcaacatcgg ctccaaggtc
     5521 acgatgaagt acccgtcgat ctacctcctg ggcaagggcg cgcggggcga ggtgctgagc
     5581 gtggcctacg ccggcaaggg gatgcacacg gacgcggggg ggaaggcgat ccacgccgcc
     5641 ccgtacacga cgagcgtcat caactcgaag tcggtctcga aggacggcgg ccgcacgggc
     5701 taccgcggcc tcgtcagggt cgaggagggc gcccatcacg ccaagtccac ggtgcggtgc
     5761 gacgcgctga tcctcgacga cgagtcggcc tcagacacgt tcccctacat cgaggtcgag
     5821 gacgacacgg ccgcgctggc ccacgaggcc acggtctcca aggtcgggga ggaccagctc
     5881 ttctacctga tgagccgggg gctctccgag caggaggcca ccgcgatggt cgtcaacggc
     5941 ttcatcgag
//
LOCUS       DASUTZ010000126         8146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_681516, whole genome shotgun sequence.
ACCESSION   DASUTZ010000126 DASUTZ010000000
VERSION     DASUTZ010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8146
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681516"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VF097_05655"
     CDS             <1..568
                     /locus_tag="VF097_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
                     /protein_id="HEX6262316.1"
                     /translation="RRDDVDLTVLRFANFIGPGVASTLTRYFSLPLIPTALGYDPRLQ
                     MIHEEDAVEALNRVVRGSYPGTFNVAADGVVLLSQALRLLGRPSLPLALPLATPVASL
                     LRRFGLVDFSTEQLRLLIYGRVADNTRMEREMGFRPARTTREALLDFAEGEHLRRLYS
                     REQVEEWEREARAFLRRKAQERFEKARS"
     gene            568..1554
                     /locus_tag="VF097_05660"
     CDS             568..1554
                     /locus_tag="VF097_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003968331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX6262317.1"
                     /translation="MGEVIPIPVREPSRCTATTRSGARCRNRAVDESGLCRVHGGGQR
                     AAAVAPEAPSTERLDELLAFLRRRVTGDYEIDEFGFDRDLTETVLAPLARFFHERYFR
                     VEWKGLEHVPREGPALLVSNHAGTIPMDAVIMKFGLLDRVGRHVRLLAANLAFRMPFV
                     GELARKSGNTLACEEDALRLLDRGELLGVFPEGYKGVGKPFRERYKLQRFGRGGFIEL
                     ALRAGAPIVPAAIVGSEEIYPLISQARPLARMFGLPYFPVTPFFPWLGPLGMLPLPTK
                     WTIEFREPIATDGMGDEAWKDSLLVFEMTDRVRDEIQQTLNRNLGSRRSLFF"
     gene            complement(1551..2645)
                     /locus_tag="VF097_05665"
     CDS             complement(1551..2645)
                     /locus_tag="VF097_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010967784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX6262318.1"
                     /translation="MTAEPRLPLGPGPSPVPPRVLEALARPTLGHLDPDFLQILDETN
                     DLLREVFRTENLAFAVSASGSGGMEAAMVNLLEPGDTVLVGVAGFFAERMAEMARRTG
                     AEVVRVEADWGRPVAIEDLEAALEGRPETKVVAVVHAETSTGVRQPLEEVAAMCRERN
                     VLLVLDCVASLGGLPVEVDRLGIDVCFSGSQKCLSVPPGLAPISYSAEALAAVEARET
                     PVQSWYFDVMGVRQYVGAADRRYHHTAPVNMIYGLNEGLKIVLEEGLEERWRRHEEVG
                     RAFQDRIQELGFELFVPEEHRLPQVTSMLIPEGADDTGARKRLIEEHGIEIAGGLGEY
                     RGRMWRVGLMGHGARMENVDRLLDAIAAVL"
     gene            complement(2642..2773)
                     /locus_tag="VF097_05670"
     CDS             complement(2642..2773)
                     /locus_tag="VF097_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262319.1"
                     /translation="MRRFTLVTLIVLLVLLAIMAVFIAIDVSRGPAPAPTPTEEGER"
     gene            complement(2802..3185)
                     /locus_tag="VF097_05675"
     CDS             complement(2802..3185)
                     /locus_tag="VF097_05675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6262320.1"
                     /translation="MSVRVLVVDDTEHVRQMMAAMLKIQGFEVVGEAGDARGALELTA
                     ETSPDVIVLDFRMPDVDGITTTRWLLEQDADLAIVIYSAYLTEEDEQRALDAGVRACV
                     SKVEGVEALAREVAAAALAVRGEQA"
     gene            complement(3182..3982)
                     /locus_tag="VF097_05680"
     CDS             complement(3182..3982)
                     /locus_tag="VF097_05680"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX6262321.1"
                     /translation="MGDGTPKLVLSTAPYYRLPLEQAFRQASAAGFEAVEVMVTQDPD
                     TQDAQTLRDLSKEHELSIEAIHAPFLLFTRRVWTTDPIEKIRRGVALAEEAEVPLLVV
                     HPPYRWQTRYRRWIGRSLAELSARSGVIIGVENMFPVWVRGGGGVRFHASQDLESLDH
                     YPTLVLDTSHLAVSGFDLLETYSRYREKIVHLHLSNNAGKGWDSHLSVDEGVLPLADL
                     LRRVGSDGFGGTISIELDLRRTIADEGATREVLVRNREFCERHLGVRA"
     gene            complement(4047..4877)
                     /locus_tag="VF097_05685"
     CDS             complement(4047..4877)
                     /locus_tag="VF097_05685"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6262322.1"
                     /translation="MVKLAFREGSGLGVLVLSRSALGALLLWGLALARGADLRVPAGR
                     WPAVWFMGALSAGVALLLFGATERIPASTTTLLLYVYPAMVAAISVALGRERPSAAKI
                     AALLVGLLGVLLVLGTPTGSLDPAGVALALASALCLALYIIVAQLGAAGMSPLVAGAV
                     ILSVSTLVYVPIAFLTGLLPGESLGGAPSFVAGVGLATGAAIALFLAGLARLEPIRAS
                     IASTWEPVAAVILSAIVLGERLSGLQLLGGVLVVAAVATLPLIRESEDATTPSGPPIA
                     "
     gene            5012..6517
                     /locus_tag="VF097_05690"
     CDS             5012..6517
                     /locus_tag="VF097_05690"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX6262323.1"
                     /translation="MESLPEIVRRAAEADPDHPALIFHDRVTTFDHLDRLVDRTAAAL
                     HDLGIGKGDRVAIMLGNIPEFVHAFHGTLRLGAVATPINAMLTPEELGYVLADAEARA
                     VIVAIDLVPAVLAVRDRLGSLDFILVVGPPPSPPGTRSFDDLLDRAKSPPPVDVRRDD
                     LAVLAYTAGTTDQPKGAMLSHGNLLANLEQISEVPMLEGAESDVALIALPLFHIYALN
                     AALNLVLMEGATAVIVDRFDAAATLQLVPHHGVTMLFGVPQMFEAWLAAARRAEPDLS
                     TVRLAVSGAAPLPGAVLEAFEERFDCTIWEGYGLTETAPVVTTNALGERAKPDSIGLP
                     LPGVEVRLLDEDGTDAVEGDPGEILVRGPNVFKGYWRRPEETDEAFTEGWLRTGDIAY
                     RDEDGYLFLVDRSKDLIIVSGFNVYPREVEEALAAHPAVAEAAVVGVSDERTGEAVRA
                     LVVAKGEVSTEELLEHCRGRLARFKLPRDIELVDSLPKHATGKVVRRSFRE"
     gene            6514..7209
                     /locus_tag="VF097_05695"
     CDS             6514..7209
                     /locus_tag="VF097_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262324.1"
                     /translation="MIAFPLLATLVALVFGVHLALRFAQRRAPHEGLWSLAMLMFAGA
                     SGALALGVQDGWSAGEFRIYWLLGAVLTVPYLAAGELYLLARQRPWIAHLGVASLAIA
                     SVWAVIEIWGAPVDGAVLEETFPLGREVFGDGTLPHRLAQYFSFPAYFFLLGGATYSA
                     GRMRGREELRDRFRGTALIAIGATIVAIGSGVGAGLGNFALFSISLAAGAAVTYWGFL
                     VATRRPTAAPAAR"
     gene            complement(7230..7982)
                     /locus_tag="VF097_05700"
     CDS             complement(7230..7982)
                     /locus_tag="VF097_05700"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX6262325.1"
                     /translation="MSWDHDHVEELLAGFALDGLDGDDRGLAERAIREHLPGCDRCRE
                     TLDHYRTIAGDLALAAPAVAAPDLLAARLSRAAASVPSRTTRRASWAIAAAAIVGFGL
                     LSGWNVMLSDRLDDAEVQQRWMAEAVATLGSPDAGVLSLEGAVDGQFQLLWAPDAERM
                     YLVASGMDSVEEGVYHVWLGNEDEWWSAGTFVPDEGVAMVPMDDDPGSFDEVAITHEE
                     TEDVPRPSATPLAHAEVAPEPTVAPSPPSAGG"
     gene            complement(7979..8137)
                     /locus_tag="VF097_05705"
     CDS             complement(7979..8137)
                     /locus_tag="VF097_05705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6262326.1"
                     /translation="MSELPPEQREALELMYFRGYTQSRIAEERGIPLGTVKTRVHAAM
                     KKLRERLT"
BASE COUNT         1140 a         2981 c         2874 g         1151 t
ORIGIN      
        1 tcgccgggac gacgtggacc tcaccgtcct tcgcttcgcc aacttcatcg ggcccggggt
       61 ggcctcgacc ctcacgcgct acttctccct cccgttgatc cccacggccc tcggctacga
      121 cccgaggctc cagatgatcc acgaggagga cgcggtggag gccctgaatc gggtggtccg
      181 cgggtcctat ccggggacct tcaacgtggc cgccgacggc gtggtgctgc tctcgcaggc
      241 cctccggctg ctggggaggc cgtccctgcc gctggccctc ccgctcgcca cgccggtcgc
      301 gtcgctgctg cgccggttcg ggctcgtcga cttctccacc gagcagcttc gcctgctgat
      361 ctacgggcgc gtggcggaca acacgaggat ggaacgggag atgggcttcc ggcccgcgcg
      421 cacgacccgc gaggccctcc tcgacttcgc cgagggcgag cacctccggc gcctctacag
      481 ccgagagcag gtcgaggagt gggagcggga ggcccgggcg ttcctgcggc ggaaggccca
      541 ggagcggttc gagaaggcga ggagctgatg ggcgaggtca tcccgatccc ggtgcgcgag
      601 ccgagccgct gcacggcgac cacgaggagc ggggcgcgct gccgcaaccg agccgtggac
      661 gagtcgggcc tgtgccgggt gcacggaggc gggcagcgcg ccgcggcggt cgctcccgaa
      721 gctccctcga ccgagcggct cgacgagctc ctcgcgttcc tgcgccgccg agtcaccggc
      781 gactacgaga tcgacgagtt cggcttcgac cgggacctca ccgagacggt cctcgcgccg
      841 ctggcccgct tcttccacga gcggtacttc cgggttgagt ggaagggtct ggagcacgtg
      901 ccgcgagagg ggcccgcgct gctcgtgtcg aaccacgccg ggaccatccc catggacgcc
      961 gtgatcatga agttcgggct gctcgaccgc gtggggcgtc acgtgcggct gctggcggcg
     1021 aacctcgcct tccggatgcc gttcgtgggg gagctcgcga ggaagtcggg caacacgctg
     1081 gcctgcgagg aggacgcgct gaggctcctc gatcgaggcg agctgctcgg cgtgttcccc
     1141 gaagggtaca aaggggtcgg caagccgttc cgcgagcggt acaagctcca gcggttcggg
     1201 cgtggaggct tcatcgagct cgccctgcgc gcgggggcgc cgatcgtgcc ggcggcgatc
     1261 gtggggagcg aggagatcta cccgctgatc tcgcaggccc gacccctggc ccggatgttc
     1321 gggctgccct acttccccgt gacgccgttc ttcccgtggc tcgggccgct cgggatgctg
     1381 cccctgccta cgaagtggac gatcgagttc cgcgagccga tcgccaccga cgggatggga
     1441 gatgaggcct ggaaggactc gctcctcgtc ttcgagatga ccgaccgggt ccgggacgag
     1501 atccagcaga cgctcaaccg caacctcggg tcccgccgct cgctgttctt ctagagaacg
     1561 gcggcgatcg cgtcgaggag gcggtcgacg ttctccatcc tcgcaccgtg gcccatcagg
     1621 cccacgcgcc acatgcggcc gcggtactcg ccgaggccgc ccgcgatctc gatcccgtgc
     1681 tcctcgatca gccgcttccg cgcgccggtg tcgtcggccc cctcggggat gagcatcgac
     1741 gtgacctggg gcagccggtg ctcctcgggg acgaagagct cgaacccgag ctcctggatc
     1801 cgatcctgga acgcccgccc gacctcctcg tgccgtcgcc accgttcctc gagcccctcc
     1861 tcgaggacga tcttcagccc ctcgttcagc ccgtagatca tgttcaccgg cgccgtgtgg
     1921 tggtagcggc ggtcggccgc ccccacgtac tgacgcaccc ccatcacgtc gaagtaccag
     1981 gactggaccg gcgtctcgcg ggcctccacc gccgccagcg cctccgccga gtacgagatc
     2041 ggggccaggc cgggcggcac tgagaggcac ttctggctcc ccgagaagca gacgtcgatg
     2101 ccgagccggt ccacctcgac cgggagccct cccagcgagg ccacgcagtc caggacgagg
     2161 agtacgttcc gctcccggca catcgcggcg acctcctcca gcggctggcg cacgccggtc
     2221 gaggtctccg cgtgcacgac cgccaccacc ttcgtctcgg ggcgtccctc gagcgcggcc
     2281 tcgaggtcct cgatcgccac gggacgtccc cagtcggcct cgacccgcac cacctcggcc
     2341 cccgtcctcc tcgccatctc cgccatgcgc tcggcgaaga acccggccac cccgacgagg
     2401 accgtgtcgc cgggctcgag gaggttgacc atcgctgcct ccatcccgcc gctgccgctc
     2461 gccgacacgg cgaaggcgag gttctccgtg cggaagacct cgcggaggag gtcgttcgtc
     2521 tcgtcgagga tctggaggaa gtcgggatcg aggtggccga gggtgggtcg agccaacgcc
     2581 tcgaggacgc gcgggggcac gggcgagggg ccgggcccga gcggcaggcg gggctcagcg
     2641 gtcatcgttc tccctcctcg gtaggcgtcg gagccggcgc cggtcctcgg gacacgtcga
     2701 tcgcgatgaa caccgccatg atcgccagca gcaccaacag cacgatcagc gtcacgagcg
     2761 tgaaccggcg caccgcccag atgctagcaa gcggtccccg ctcaggcctg ctcgccgcgg
     2821 acggcgagcg ccgccgcggc cacctcgcgg gccagcgcct ccacgccctc caccttggag
     2881 acgcaggcgc ggaccccggc gtccagcgcc cgctgctcgt cctcctccgt gaggtaggcc
     2941 gagtagatga cgatcgccag gtcggcatcc tgctcgagca gccaccgcgt cgtggtgatc
     3001 ccgtccacgt cgggcatgcg gaagtcgagg acgatgacgt cgggcgacgt ctcggccgtc
     3061 agctccagtg ctcccctcgc gtcgcccgcc tcgcccacca cctcgaagcc ctggatcttc
     3121 agcatcgccg ccatcatctg gcggacgtgc tcggtgtcgt ccacgacgag cacccggacg
     3181 ctcacgcccg caccccgaga tgccgctcgc agaactctcg gttccgcacg aggacctcgc
     3241 gcgtcgcgcc ctcgtccgcg atcgtcctgc ggaggtccag ctcgatcgag atcgttccgc
     3301 cgaacccgtc cgagccgacc cggcgcagca gatcggcgag cgggaggacg ccctcgtcca
     3361 cggagaggtg cgagtcccag cccttccccg cgttgttgga gaggtggagg tggacgatct
     3421 tctcccggta ccgggagtac gtctccagga ggtcgaagcc cgagacggcc aggtggctcg
     3481 tgtcgagcac gagggtgggg tagtggtcga ggctctccag gtcctgggag gcgtggaagc
     3541 gaacgccgcc ccccccgcgg acccacacgg ggaacatgtt ctccacgccg atgatcacgc
     3601 cggagcgggc ggagagctcg gccaggctcc ggccgatcca ccgccggtag cgggtctgcc
     3661 accggtaggg tgggtggacg acgaggagcg gcacctcggc ctcctccgcc agcgcgaccc
     3721 cgcgcctgat cttctcgatc ggatcggtgg tccagacccg ccgtgtgaag aggaggaagg
     3781 gcgcgtggat cgcctcgatc gagagctcgt gctccttgga caggtcgcgg agggtctggg
     3841 cgtcctgcgt atccgggtcc tgggtcacca tgacctcgac cgcctcgaag ccggccgcgc
     3901 tggcctgcct gaaggcctgc tccagcggca gtcggtagta cggggcggtc gagaggacca
     3961 gcttgggcgt tccgtctccc acgttcctca tcctctccgg agtcgtcatt ctacagtggc
     4021 cccccgcggg ttcgaggacg ctcgcttcag gcgatgggag gcccggacgg ggtggtggcg
     4081 tcctcggact cccggatcag ggggagcgtc gccacggcgg ccacgaccag cacgccgccg
     4141 aggagctgaa ggcccgacag tcgctcgccc aggacgatcg ccgagaggat cacggcggcc
     4201 acgggctccc aggtggacgc gatcgaggcc cggatcggct cgagccgggc cagccccgcc
     4261 aggaacaggg cgatggcggc tcccgtggcc aggccgaccc cggccacgaa ggagggcgcg
     4321 ccgccgaggc tctcacccgg cagcagcccg gtgaggaacg cgatggggac gtagaccagc
     4381 gtggagaccg agaggatcac ggcgccggcc accaggggcg acatcccggc cgcgccgagc
     4441 tgggccacga tgatgtagag ggccaggcag agggccgagg ccagggccag ggccacgccc
     4501 gccgggtcga ggctgccggt gggggtccca aggacgagga ggacgcccag gaggcccacc
     4561 aggagggcgg cgatcttcgc cgcgctcggg cgctcccgcc cgagcgcgac ggagatcgcc
     4621 gccaccatcg ccgggtacac gtacagcagc agcgtcgtcg tggaggccgg gatccgctcg
     4681 gtggcgccaa acaggagcag cgcgaccccg gccgacagcg cgcccatgaa ccacaccgcc
     4741 ggccaccgcc cggcggggac ccggagatcc gccccccgag ccagggccag gccccagagc
     4801 aggagcgcgc cgagcgccga ccgcgacagc acgaggaccc ccagacccga gccctcgcgg
     4861 aaggccaact tgaccatcac ggtggccgac gcgaagctcg tcgccgagag ggccacgagg
     4921 acgatgccga tcggtcgggt gctcgtgcgc gagacgctcc tcctccctcc ctcgtgccgg
     4981 accgaccgac cctagcctag actcacgtcc gatggagtca ctgcccgaga tcgtccggcg
     5041 agcggcggag gcggatccgg atcacccggc cctgatcttc cacgatcggg tgaccacctt
     5101 cgaccatctc gaccgcctgg tggaccgcac ggcggcagcc ctccacgacc tcgggatcgg
     5161 gaagggcgac cgcgtggcca tcatgctcgg gaacatccca gagttcgtcc acgccttcca
     5221 cgggaccctg cgcctcgggg ccgtggccac cccgatcaat gcgatgctca cccccgagga
     5281 gctcgggtac gtcctggccg acgccgaggc ccgggcggtc atcgtggcca tcgacctggt
     5341 gccggcggtc ctggccgtgc gggaccggct gggctccctc gacttcatcc tggtggtggg
     5401 cccgcccccc tcccccccgg ggacccgctc cttcgacgac ctcctcgacc gggccaagag
     5461 cccgcctccc gtggacgtgc gtcgggacga cctcgccgtg ctcgcctaca ccgccgggac
     5521 caccgaccag cccaagggcg cgatgctgag ccacggcaac ctcctggcga acctcgagca
     5581 gatctccgag gtcccgatgc tggagggagc cgagtcggac gtggccctga tcgccctgcc
     5641 gctcttccac atctacgcgc tcaacgccgc gctcaacctc gttctgatgg agggggccac
     5701 tgcggtcatc gtggaccgct tcgacgccgc cgccacgctc cagctcgtgc cccaccacgg
     5761 cgtcacgatg ctcttcgggg tgccccagat gttcgaggcc tggctggccg ccgcccgcag
     5821 ggccgagccc gatctctcca ccgtgaggct ggccgtctcg ggcgcggccc cccttcccgg
     5881 ggccgtcctg gaggcgttcg aggagcggtt cgactgcacg atctgggagg gatacggcct
     5941 caccgagacc gcccccgtcg tcaccacgaa cgcgctcggc gagcgagcca agccggactc
     6001 gatcgggctg ccgctccccg gcgtcgaggt gcgtctcctc gacgaggacg ggaccgacgc
     6061 ggtggagggc gacccgggcg agatcctcgt ccgcggcccc aacgtcttca agggctactg
     6121 gcggcgcccc gaggagacgg acgaggcgtt caccgagggc tggctccgca ccggcgacat
     6181 cgcctaccgg gacgaggacg gctacctgtt cctcgtggac cggtcgaagg acctcatcat
     6241 cgtgtccggt ttcaacgtgt acccgaggga ggtggaggag gcgctcgccg cgcaccccgc
     6301 cgtggccgag gccgcggtgg tcggggtgtc cgacgagcgg accggcgagg cggtccgggc
     6361 gctcgtggtg gcgaaggggg aggtctcgac cgaggagctg ctcgagcact gccgcgggcg
     6421 gctggcccgc ttcaagctcc cccgcgacat cgagctcgtg gactcgctcc ccaagcacgc
     6481 caccgggaag gtcgtgcggc ggagcttccg cgagtgatcg ccttcccgct gctggcgacg
     6541 ctggtggcgc tggtgttcgg cgtccacctg gccctgcgat tcgcgcagcg gcgggcgccc
     6601 cacgaggggc tgtggtcgct ggcgatgctg atgttcgcgg gggcgtcggg cgcgctggcg
     6661 ctcggggtcc aggacgggtg gtcggcgggg gagttccgca tctactggct gctgggggcg
     6721 gtgctgacgg tgccgtacct cgcggcgggg gagctgtacc tgctggcgcg ccagcgacca
     6781 tggatcgcgc acctcggggt cgcgtcgctc gcgatcgcca gcgtatgggc ggtgatcgag
     6841 atctgggggg cgccggtcga tggcgcggtg ctggaggaga cgttcccgct ggggcgcgag
     6901 gtcttcggcg acggaacgct cccgcaccgg ctcgcgcagt acttctcgtt cccagcgtac
     6961 ttcttcctcc tgggcggggc cacctactcg gcgggccgga tgcggggcag ggaggagctc
     7021 cgggaccggt tccgggggac cgcgctgatc gcgatcggcg ccacgatcgt ggcgatcggc
     7081 tcgggcgtgg gtgcggggct gggcaacttc gcgctgttct cgatctcgct cgcggccggc
     7141 gcggcggtca cgtactgggg cttcctcgtg gccacccgcc gcccgaccgc cgcgccggcc
     7201 gctcgctgag ccagggggcc gctgccgcgt cagccgccgg ccgacggggg ggagggggcg
     7261 acggtcggct cgggggccac ctccgcgtgc gccagcgggg tggccgacgg cctcgggacg
     7321 tcctccgtct cctcgtgggt gatcgccacc tcgtcgaatg agcccggatc gtcgtccatc
     7381 gggaccatcg ccacgccctc gtcggggacg aacgttcctg cactccacca ctcgtcctcg
     7441 ttccccagcc agacgtggta aacgccctcc tccaccgagt ccatccccga ggcaacgagg
     7501 tacatccgtt cggcgtcggg ggcccacagc agctggaact gcccgtccac cgccccctcg
     7561 agcgagagga cgccggcgtc cgggctcccc agcgtggcca ccgcctcggc catccagcgc
     7621 tgctggacct cggcgtcgtc gagacggtcc gacagcatca cgttccagcc cgagagcagg
     7681 ccgaagccca cgatcgcggc cgcggcaatg gcccagctcg cgcgacgggt cgtccggctc
     7741 ggcacagagg ccgcggcacg ggagagccgt gcggccagga ggtcgggcgc cgccacggcg
     7801 ggcgccgcca gggccaggtc ccccgcgatc gtccggtagt ggtcgagcgt ctcgcgacac
     7861 cgatcgcacc cggggaggtg ctcgcggatc gcccgctcgg ccaggcctcg gtcgtcgccg
     7921 tcgagcccgt cgagcgcgaa gcccgcgagc agctcctcga cgtggtcgtg gtcccagctc
     7981 acgtcagccg ctcccgcagc ttcttcatgg cggcgtgcac cctcgtcttc accgttccca
     8041 aggggatccc tcgctcctcc gcgatcctcg actgcgtgta gcccctgaag tacatcagct
     8101 ccagcgcctc gcgctgctcg ggagggagct cggacagggc ctcccg
//
LOCUS       DASUTZ010000127         3830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_552024, whole genome shotgun sequence.
ACCESSION   DASUTZ010000127 DASUTZ010000000
VERSION     DASUTZ010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552024"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VF097_05710"
     CDS             complement(<1..326)
                     /locus_tag="VF097_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262327.1"
                     /translation="MPEPVVLVWGESDFLLREAATRLFEGARPTEVEGAAWRPGLTAD
                     LATPSLLGEERALLVTGLQAAPSEALDEIARYALEPNPEARLVLTCAVTPRAKGPPAK
                     VMKLLQG"
     gene            complement(374..778)
                     /locus_tag="VF097_05715"
     CDS             complement(374..778)
                     /locus_tag="VF097_05715"
                     /inference="COORDINATES: protein motif:HMM:NF025066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEX6262328.1"
                     /translation="MSDQKLSIVLFSGTDDKLLGATHLVAGATGLGREVDVFLQFWAV
                     DAFRHDHINKDHGVTVDAGPEGPEMLERYGEIRWVETLHSVKEAGTLRITACSRCMEA
                     MGLAAEDMDELVDEVEGVTAFLSRATGQLIHM"
     gene            complement(845..3166)
                     /locus_tag="VF097_05720"
     CDS             complement(845..3166)
                     /locus_tag="VF097_05720"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX6262329.1"
                     /translation="MVAVALGAVALWAGLLLGWGRIEPSAGGWMLALGAGAVLLVGRR
                     RRGSSRDAAALGERERVLAAAGFLSRPPHVGRPVLRTMALAAATLLIGLAWAGLRLRS
                     PPDLGPLEGRSAPFLGTARSDPRSFPTGWGVEVRVDRAGDRPLGHLVWVSGRGEPPQV
                     AAGDRVRGAGRFRRVEGEEGFERYLTDRGVAALVSASELGREGPPRNPLLRVANEVRD
                     ALAGGARRALPPRRAGLLLGLTIGDTRGMHPETVEDFRATGLGHLVAVSGSNVVMVLA
                     PVVGLAALLGMGPRGRLVSGGLAVVLFALITRWEPSVLRAGVMAGLALLGVAAGRPRE
                     TATVLSWAVVILLMVDPRLSRSLAFQLSTAATVGLVALAPGIEARLGWLPRPVALAAA
                     ATAGAQAAVTPLLLAAFGIVPGSTLIANVLAFPAVAAAFLGGSTAAAVALVWPGMGSA
                     LGALASMPIAYLEAVADRTARLAVPALVGTDWVPPVALVVVAHLVLRRVVAPRRLAVL
                     LAAPFLVWVVFVRGAPPPGLEVRFLDVGQGDAALVRTPRATILVDAGPDEQTVATRLA
                     ALGIGRLDLAVASHGHQDHVEGFPAVFARHPVGLLLDPGCPTGSPSHERMLDAAVYED
                     VPIRHPRGGERLRVGDLLLEVLGPDECAPDSPNDDSIVLRLLHGSATVLLPGDAEVPA
                     QLDLLSDADPVRATVLKVPHHGSSTSDPSFFEAVAASVAVVSVGEDNDFGHPVPDTLR
                     ALRAAGGRVVRTDLAGEVTVTFEGDRPVVERSR"
     gene            complement(3166..>3830)
                     /locus_tag="VF097_05725"
     CDS             complement(3166..>3830)
                     /locus_tag="VF097_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEX6262330.1"
                     /translation="AGTMRASPSRDAAGRRASMDGFRDWLEGLTARELAVYGVVALAL
                     LVAGGAWVLRGRPSATPIQGPAPRAGPSPEAEGAPVIVHVAGMVRTPGVYQLAEGDRV
                     IDAIEMAGGARRGAALEALNLAAVLTDGQQILVPKRGAAPDPAAPSAGGSSGSTSNLV
                     NLNTADATTLETLPGIGEVLAQRIIEHREANGSFASVDDLISVSGIGEVTLEELRPLV
                     TV"
BASE COUNT          552 a         1514 c         1358 g          406 t
ORIGIN      
        1 ccctggagga gcttcatgac cttggccggg gggcccttgg cgcgcggggt gacggcgcac
       61 gtgaggacga gccttgcctc cgggttcggc tccagcgcgt agcgcgcgat ctcgtcgagg
      121 gcctcggagg gcgccgcctg gaggcccgtc accagcaggg cccgctcctc cccgaggagg
      181 gagggcgtcg ccaggtcggc cgtgaggccg ggcctccagg cggcgccctc cacctccgtg
      241 gggcgggccc cctcgaacag cctcgtggcg gcctcgcgga gcaggaagtc gctctcgccc
      301 cagacgagga ccaccggctc aggcactcag catcacgggc ggatgctacc tcgccgggag
      361 caccccggcc gcgtcacatg tggatcagct ggcccgtggc gcgggacagg aacgcggtca
      421 cgccctcgac ctcgtccacg agctcgtcca tgtcctcggc agcgagcccc atggcctcca
      481 tgcagcgcga gcacgccgtg atgcggagcg tgccggcctc cttgacgctg tggagggtct
      541 ccacccagcg gatctccccg taccgctcga gcatctcggg cccctcggga ccggcgtcca
      601 ccgtcacacc gtggtccttg ttgatgtggt cgtgacggaa cgcgtccacg gcccagaact
      661 ggaggaagac gtcgacctcc cggcccaggc ccgtggcccc cgccaccagg tgcgtggccc
      721 cgaggagctt gtcgtcggtg cccgagaaca gcacgatcga gagcttctgg tccgacatgc
      781 caccacctcc agcgttcagc ctggcaggcg gcggcgtccc cgggccgggc cgtgaggccc
      841 gcgttcaccg ggacctctcg accaccggcc ggtcgccctc gaaggtcacg gtcacctcgc
      901 cggccaggtc ggtccgcacg acccgtccgc ccgcagcacg cagcgcccgg agcgtgtccg
      961 gcacggggtg gccgaagtcg ttgtcctcgc ccacgctgac gacggcgacc gaggccgcga
     1021 ccgcctcgaa gaaggacgga tccgacgtgg aggagccgtg gtgcgggacc ttgaggaccg
     1081 tggcccgcac ggggtcggca tccgacagca ggtcgagctg ggccgggacc tccgcgtcgc
     1141 ccggcaggag gaccgtggcc gacccgtgga ggaggcgcag gacgatcgag tcgtcgttcg
     1201 gggagtcggg cgcacactcg tcgggaccca ggacctcgag caggaggtcc ccgacccgca
     1261 ggcgctcccc gccgcgcggg tgccggatcg ggacgtcctc gtacaccgcg gcatcgagca
     1321 tccgctcgtg ggaaggggat ccggtcgggc accccgggtc gaggaggagc ccgacggggt
     1381 gtcgggcgaa cacggccggg aacccctcga catggtcctg atgaccgtgt gaggccacgg
     1441 caaggtcgag gcgtccgatc ccgagggcgg cgaggcgggt ggccacggtc tgctcgtcgg
     1501 gcccggcgtc caccaggatc gtggcccggg gcgtccggac gagcgccgcg tcgccctgcc
     1561 ccacgtcgag gaaccgcacc tcgaggcccg gcggcggggc gccgcggacg aacacgaccc
     1621 agacgaggaa gggcgcagcc agcagcacgg cgaggcggcg tggggcgacc acgcgccgca
     1681 ggacgaggtg ggccacgacc accagggcga ccgggggcac ccagtcggtg cccaccagcg
     1741 cggggacggc cagcctcgcc gtgcggtcgg caacggcctc cagataggcg atcggcatcg
     1801 aggcgagcgc gccgagggcg gagcccatcc cgggccagac gagggcgacg gccgcggccg
     1861 tcgagccgcc caggaaggcc gccgccacgg cggggaaggc caggacgttg gcgatcaggg
     1921 tcgagcccgg cacgatcccg aacgctgcca gcaggagggg ggtcacggcc gcctgcgctc
     1981 ccgccgtggc ggcggccgcc agggccaccg gacgggggag ccatcccagc cgcgcctcga
     2041 tcccgggcgc gagcgccacg agcccaaccg tcgcggcggt cgagagctgg aacgccagcg
     2101 atcgcgagag cctgggatcg accatcagca ggatcaccac cgcccaagac aggacggtgg
     2161 ccgtctcccg gggccgcccc gccgccaccc ccagcagggc caggccggcc atcacaccgg
     2221 cgcgcaggac cgagggctcc cacctcgtga tgagggcgaa gaggacgacc gcgagtcccc
     2281 ccgacacgag cctcccccgc gggcccatcc ccagcagcgc ggccaggccc accaccggcg
     2341 ccaggaccat caccacgttc gatcccgaga ccgccacgag gtgccccagg cccgtggccc
     2401 ggaagtcctc cacggtctcc gggtgcatcc cgcgcgtgtc cccgatcgtc aggccgagca
     2461 gcagtcccgc tctccggggc gggagggccc gccgcgcccc gccggccagc gcgtcgcgga
     2521 cctcgttcgc gactcggagc agcgggttgc gcggcgggcc ctcccgcccc agctccgacg
     2581 ccgacacaag tgcggccacc cctcgatcgg tcaggtatcg ctcgaaaccc tcctcccctt
     2641 cgacgcggcg gaagcggccc gcgccgcgca cccggtcccc cgccgccacc tgcgggggct
     2701 ccccccgacc cgagacccac accaggtgtc ccagggggcg atccccggcc cgatcgacgc
     2761 ggacctccac cccccagccg gtggggaagc tcctcgggtc ggagcgcgcc gttccgagga
     2821 acggcgcgct ccgaccctcc agcggcccca ggtcgggggg tgagcggaga cgcagcccgg
     2881 cccacgccag cccgatcagc agagtcgccg ccgccagcgc catggtgcgg agcacgggcc
     2941 gcccgacatg cggcggccgt gagaggaacc ccgccgccgc cagcacccgc tcgcgctccc
     3001 ccagcgcggc ggcgtcccgt gagctcccgc gtcgacgccg cccgaccagc aggacggccc
     3061 cggcgccgag cgcgagcatc caccctcctg cggatggctc gatccgcccc caccccagca
     3121 ggagccccgc ccacagggcc acggcgccga gcgcaaccgc caccatcaca ccgtcaccag
     3181 cggccgcagc tcttcaagcg tgacctcgcc gatgccgctc accgagatga ggtcgtccac
     3241 cgaggcgaac gacccattcg cctcccggtg ctcgatgatc cgctgggcca gcacctcccc
     3301 gatccccggc aaggtctcca gcgtcgtcgc gtccgccgtg ttcaggttga cgaggttcga
     3361 tgttgacccc gatgagcccc ccgccgaggg cgcggccgga tcgggcgccg ccccgcgctt
     3421 ggggacgagg atctgctgcc cgtccgtcag taccgccgcc aggttgagcg cctcgagggc
     3481 ggcgcccctg cgagcccctc ccgccatctc gatcgcgtcg atcacgcggt cgccctcggc
     3541 gagctggtag acgccgggcg tccgcaccat cccggcgacg tgcacgataa ccggagcccc
     3601 ctccgcctcc ggcgacggcc ccgcccgtgg cgccggtccc tggatggggg tggccgaagg
     3661 ccgcccacgc aggacccacg ccccgccggc cacgagcagc gccagcgcga cgaccccgta
     3721 caccgcgagc tccctcgcgg tcagcccctc cagccagtcg cggaacccgt ccatggaagc
     3781 gcggcgtccg gccgcgtctc gggaggggga ggcccgcatc gtaccggccg
//
LOCUS       DASUTZ010000128         3792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_422429, whole genome shotgun sequence.
ACCESSION   DASUTZ010000128 DASUTZ010000000
VERSION     DASUTZ010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422429"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VF097_05730"
     CDS             <1..228
                     /locus_tag="VF097_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010034071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX6262331.1"
                     /translation="AEAYPMSFAAVQKHVAVLERAGLVTKERRGREHLVHTVPEAVRR
                     ARRALDRLEATWRERVERISDLLDETKGRER"
     gene            225..710
                     /locus_tag="VF097_05735"
     CDS             225..710
                     /locus_tag="VF097_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010034074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX6262332.1"
                     /translation="MSVTSIDKDLDGLTFTLIADFEAPMERVWELWADPRKLERWWGP
                     PGYPATFERHDLTPGGEVAYYMTSPEGERYRGWWRIVSVDAPKALEFEDGFADEEGNP
                     IADMPISAVRMQLTEHEGGTRMELRSTFDSREDMERVMELGAAEGIEQAVGQMDALLG
                     S"
     gene            736..1347
                     /locus_tag="VF097_05740"
     CDS             736..1347
                     /locus_tag="VF097_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015810855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6262333.1"
                     /translation="MRDLVVQTFLTLDGVMQAPGGPEEDDSGGFAHGGWSVNYWDEQM
                     GRVMAEATSRPFAMVLGRRTYDIMAAHWPNAPEEEGARTFNEATKYVASRGRPTLEWS
                     NSVLIEGDVAAGIAALKEEDGPELQVHGSANLIQTLLRHGLVDRFRLWIFPLVIGSGK
                     RLFGDGTMPSGLKLVDQEVSSTGVVMALYEPAGEIVTGSFALD"
     gene            1402..>3792
                     /locus_tag="VF097_05745"
     CDS             1402..>3792
                     /locus_tag="VF097_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6262334.1"
                     /translation="MGELPTGTVTFLFTDIEGSTRLLQELGDAYATVQGEHDAIVSRA
                     VEASGGHHVRSEGDAFFVVFQRPAGALEAAVAAQRSLAEHDWSHGAPLRVRMALHTGE
                     GVLGGADYVGIDVNRAARIAAAAHGGQILLSEATRGLVEHALPGGVSLRGLGEHRLKD
                     IAHPERLHDVVIEGLPSDFPPLRTLDARPNNLPVQLTSFVGREHEIEEATRLLRRTRL
                     LTFTGPGGSGKTRLALQVAAETISDYEDGAFFVDLSTYTDPALVPSAIAGVLGVPEEP
                     GRPLSEGLKDHVRGLNLLLILDNFEQVVEAAPIVEELLTAAPRLRVIVTSRTPLGVRG
                     EHERPVPPFQPPDPEHLPDLPALCETEAVRLFTERALAVNPAFEVSEENAEEVAEVLV
                     RLDGLPLAIELAATRTKLLAPQQMIERLDHRLALLESGARTLPERQRTLRGAIAWSYD
                     LLVEAERRMFARLSIFAGGWTLTSAEAVCDTAGLGLDPLDSMTSLVDKSLIRLAEASE
                     GDPRFSMLETIREFGREQLEALGELDDVRRRHAELFLDLAQEVEPHLMAEDQGEWLDR
                     CDREQANIRRALRRAIEQGELARAQEAAGALWRFWHQRGHLAEGRRWFEELLAHPDGQ
                     GPTAARAKALTGAGGIAWWQADVPAARGFYEESLAIERGRGSPVRIAAAAYDMAHAAI
                     AGDEFEDALRLLEEALSLFREVGDELGATRTLSALALADAIMGNWEAAVRRQEEGVET
                     YRRLGDPFHLANGLSNLGVGYGRVGRWDESRTAIHEALELFSAARNPTGIASVILDL"
BASE COUNT          574 a         1259 c         1413 g          546 t
ORIGIN      
        1 gccgaggcct accccatgag cttcgccgcg gtgcagaagc acgtcgccgt gctggagcgg
       61 gccggcctcg tcacgaagga gcggcgcggg cgcgagcacc tcgtgcacac ggtccccgag
      121 gcggtgcgcc gagcccgccg cgccctcgat cggctcgagg cgacctggcg ggagcgggta
      181 gagaggatat cggacctgct cgacgagacg aaaggacgag agcgatgagc gtcaccagca
      241 tcgacaagga cctggacggc ttgaccttca ccctgatcgc ggacttcgag gcgccgatgg
      301 agcgtgtttg ggagctgtgg gccgacccgc ggaagctgga gcggtggtgg ggacctcccg
      361 gatacccggc gaccttcgag cggcacgacc tgacgccggg cggcgaggtc gcctactaca
      421 tgacgagccc ggagggcgag cggtaccggg gctggtggcg gatcgtctcg gtcgacgccc
      481 cgaaggcgct ggagttcgag gacgggttcg ccgatgagga ggggaacccg atcgccgaca
      541 tgccgatcag cgccgtgcgc atgcagctca cggagcacga gggcgggaca cgtatggagc
      601 tgcgttcgac cttcgactcc cgtgaggaca tggagcgcgt catggagctg ggggcggccg
      661 agggcatcga gcaggcggtg ggccagatgg acgccctcct cggttcctga ccccgacacg
      721 aagggataag gcctgatgag agacctcgtc gtacagacgt tcctgaccct ggacggggtc
      781 atgcaggcgc cgggcggccc cgaggaggac gacagcggcg gcttcgccca cggcggctgg
      841 tcggtcaact actgggacga gcagatgggc cgagtcatgg cggaggcgac gagccggccg
      901 ttcgccatgg tcctcgggcg acggacctac gacatcatgg cggcgcactg gccgaacgcg
      961 cccgaggagg agggcgcgag gaccttcaac gaggcgacga agtacgtggc ctcgcgtggc
     1021 cgccccaccc tggagtggag caactccgtc ctgatcgagg gagatgtcgc ggctggcatc
     1081 gcggcgctca aggaggagga cgggccggag ctccaggtgc acggcagcgc gaacctgatc
     1141 cagacgctgc tgcgccacgg gctcgtggat cggttccgtc tctggatctt ccccctcgtg
     1201 atcgggtcgg ggaagcgtct gttcggggac gggacgatgc cctccgggct gaagcttgtc
     1261 gaccaggagg tctcctccac gggcgtggtc atggccctct acgagcccgc gggcgagatc
     1321 gtcacgggtt cgttcgcgct ggactgacat cgctcttcgg gcccggcccc gaggccgcga
     1381 acgcgctaca tttcgtccgc catgggagag ctcccgaccg ggacggtcac cttcctgttc
     1441 accgacatcg agggctcgac ccggctgctc caggagctcg gcgacgccta cgcgaccgtt
     1501 cagggcgagc acgacgccat cgtgtctcgg gccgtggagg cctccggcgg gcaccacgtt
     1561 cgcagcgagg gggacgcgtt cttcgtcgtc ttccagaggc cggccggggc gctcgaggcc
     1621 gcggtcgccg cccagcggag cctcgccgag cacgactggt cccacggtgc gcccctccgc
     1681 gtccggatgg cccttcacac gggcgagggg gtcctgggcg gcgccgacta cgtgggcatc
     1741 gacgtgaaca gggccgcgcg gatcgcggcg gcggcgcacg gcggtcagat cctgctctcg
     1801 gaggccacgc ggggcctcgt agagcatgcc ctcccggggg gcgtctcgct gcgcggcctc
     1861 ggggaacaca ggctcaagga catcgcccat cctgagcgcc tccacgacgt ggtgatcgag
     1921 ggcctgccgt cggacttccc tccgctccgg accctcgacg cgcgcccgaa caacctaccg
     1981 gtccagctca cgtccttcgt cgggagggag catgagatcg aggaagcgac ccgtctcctg
     2041 cggcggacgc gtctgctgac gttcaccggc ccgggaggca gcgggaagac ccggctggcc
     2101 ctccaggtgg cggccgagac gatctccgat tacgaggacg gggcgttctt cgtcgacctc
     2161 tcgacctaca cggatccggc cctcgtcccc tccgcgatcg ccggcgtcct gggcgttccc
     2221 gaggagccgg gccgcccgct gagcgagggc ctgaaggatc acgtgcgcgg cctcaatctc
     2281 ctcctgatcc tcgacaactt cgagcaggtc gtggaagcgg ctcccatcgt cgaggagctt
     2341 ctgacggccg cccccaggct cagggtgatc gtcacctccc gcacgccgct cggggtgcgc
     2401 ggtgagcacg agcggcccgt gccgccgttc cagccgcccg atcccgagca cctcccggat
     2461 ctccccgcgc tctgcgagac cgaggccgtg cgactcttca ccgagcgagc cctggccgtg
     2521 aacccggcct tcgaagtcag cgaggagaac gccgaggagg tggcggaggt cctggtccgc
     2581 ctcgacggcc tcccgctggc catcgagctc gcggcgaccc ggacgaagct cctcgctccg
     2641 cagcagatga tcgagcggct cgaccatcga ctggcgctgc tggagtcggg cgctcggacg
     2701 ttgcccgagc gccagcgcac cctccgaggt gcgatcgcct ggagctacga cctgctcgtg
     2761 gaggccgagc ggaggatgtt cgcccggctg tcgatcttcg ccggtggctg gaccctgacg
     2821 tccgcggagg ccgtgtgcga cacggcgggc ctcggcctgg atcctctgga ttcgatgacc
     2881 tccctcgtgg acaagtcgct gatccggttg gccgaggcct cggaaggcga tcctcggttc
     2941 tcgatgctgg agacgatccg ggagttcggc cgagagcagc tcgaggcgct gggagagctc
     3001 gacgacgttc gccggcgcca cgccgagctc ttcctcgacc tggcacagga ggtcgagcca
     3061 catctgatgg ccgaggacca aggcgaatgg ctcgatcgct gcgaccggga gcaggcgaac
     3121 atccggaggg ccctgcggcg ggcgatcgag cagggcgagc tcgctcgggc gcaggaggcg
     3181 gccggagcgc tgtggaggtt ttggcaccag cgggggcatc tcgcggaggg ccggcggtgg
     3241 ttcgaggagc ttctggcgca cccggacggg caaggaccga cggcggccag ggcgaaagcc
     3301 ctcacggggg cgggcgggat cgcgtggtgg caggcggacg tgcccgccgc gcgggggttc
     3361 tacgaggagt ccctcgcgat cgagcgaggg cgggggagcc cggttcggat cgcggcggcc
     3421 gcgtacgaca tggcgcacgc ggccatcgcc ggtgacgagt tcgaggatgc tctgcggctg
     3481 ctggaggaag cgctcagtct cttccgtgag gtgggagacg agcttggagc cacgcggacc
     3541 ctctccgctc tggcgttggc cgacgcgatc atgggcaact gggaggccgc ggtgagacgg
     3601 caggaggagg gcgtcgagac ctaccggagg ctgggcgacc cctttcacct cgcgaacggg
     3661 ctgtcgaacc tcggggtcgg ttacgggcgg gtcggaaggt gggacgagag ccggaccgcg
     3721 atccacgagg ctctcgagct gttctctgcc gcgcggaacc cgaccgggat cgcctccgtg
     3781 atcctcgacc tc
//
LOCUS       DASUTZ010000129         8054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_163076, whole genome shotgun sequence.
ACCESSION   DASUTZ010000129 DASUTZ010000000
VERSION     DASUTZ010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8054
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163076"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VF097_05750"
     CDS             complement(<1..306)
                     /locus_tag="VF097_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX6262335.1"
                     /translation="MAITIALAGDTMLGRGVAERIEERGPRSLVAPEVASLMREADLC
                     ILNLECCISDRGEPWPAPGKPFFFRAPATAVETLVDLGVDAVTLANNHALDFGEVALL
                     "
     gene            complement(311..1312)
                     /locus_tag="VF097_05755"
     CDS             complement(311..1312)
                     /locus_tag="VF097_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HEX6262336.1"
                     /translation="MEIEATDYHTAGEPFRIVTGGAPALEGGSILDKRAFAAEHLDEV
                     RQLLIQEPRGHADMYGCFVTDPEDEGADLGVVFFHNAGYSTACGHGTIALVTWALESG
                     RLEREGLPVEDDEVRVVVDVPSGRLPTWARLEGDRVRSVRFRNVPSFCRARGIEVTGV
                     TADVSYGGAFYASVAAADAGLTVEPEHTERFIALGREIQAALNERRIAEHPAEGRLSG
                     VYGVIFHEEAPEPEARVHQRNVAIFADGEVDRSPCGSGTSARLAVLHADGGIERGEWM
                     LHDGIVGTRFRGRVVEETEIHRLPAVVTEVEGSAHRSGEHRFVLEEGDPLGTGFLLR"
     gene            1344..2063
                     /locus_tag="VF097_05760"
     CDS             1344..2063
                     /locus_tag="VF097_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020557102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEX6262337.1"
                     /translation="MDRRERYRAARRDPSLAVLEGIHAVKHAIRFGAELLDVVASDES
                     PLEELAADLAPDAAGRLRAVERIPAEGFAELSPTPPATGVVALARRPSVDMAAVLADP
                     GPWPVIFLEEPRRLENVGAVVRVAAAAGAAGVLTTGRHDPWHPAALRGSAGLHFAVPV
                     ARVETLPVSDRPLVALDPEGIELGPGVLPSRTVAAFGTERAGLSEGVLRRADLRVRIP
                     MRDGVSSLNLATAVAIVLYAR"
     gene            complement(2051..2494)
                     /locus_tag="VF097_05765"
     CDS             complement(2051..2494)
                     /locus_tag="VF097_05765"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX6262338.1"
                     /translation="MPAVEGGVRGEGVRPAVVLLGIAVWLGARALGELGPAEASEPER
                     GGLLRIFSGFLGLTLLNPVTSVYFTALILGLRGGELEAGSAKVAFVAGAFLASLSWQW
                     LLAGAGSVLRHRLPTRARRVTSLLGGAIVALLALGSSWADPRQRA"
     gene            2557..3006
                     /locus_tag="VF097_05770"
     CDS             2557..3006
                     /locus_tag="VF097_05770"
                     /inference="COORDINATES: protein motif:HMM:NF018595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HEX6262339.1"
                     /translation="MAQARKQPGVERIYRDDQIEVTWEPSLCIHYGACYGQLPRVFRP
                     RERPWVRLEEASADEIGKVVQDCPTGALGFRRLDGGPQEEVPEQAEIRVDPKGPIYLR
                     GRVRIEREGEVLREGTRVALCRCGQSGNKPFCDGTHLKVGFTDEPEA"
     gene            3047..3946
                     /locus_tag="VF097_05775"
     CDS             3047..3946
                     /locus_tag="VF097_05775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6262340.1"
                     /translation="MRVGVQLPEVEREVRWPEYVAMARAAEEVGFDSLWVGDHLLYRD
                     DGRPERGPWDAWALLAGLAAVTERARLGPLVACTAFRPPGVLARTAAAVHELSGGRLV
                     LGLGAGWNEEEFRAFGIPFDHRASRFEEAFEIVRRLLAGERVTFPGRFHEAEDAVLLP
                     RPSQRIPLMIGSTGRRVLAAALPHVDVWNTWYDWYGNTPEGFARGNAEIDRAVERVGR
                     EPEAVERSACVFIVVDEEAGERPAQEGVTPLTGGPEKVGAGLREIAEAGADELILVLD
                     PIDERSIRFCGEAIALVRGRPPG"
     gene            complement(3958..4608)
                     /locus_tag="VF097_05780"
     CDS             complement(3958..4608)
                     /locus_tag="VF097_05780"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6262341.1"
                     /translation="MLASGAMFELDHVILGVVDLPAASARLRTDLGLASVEGGRHVGV
                     GTGNRIVPLGRNYVEVLAVADPEEARAHPFGRWVLDYLGGRERFLGWCLRTDGLDVIA
                     KRLGLEIEAWSRERPDGAVLRWRMAGLDVAMKDPSLPFFMEWEIEDDLYPGRTRAAHA
                     AEPKGFAWVEVAGDAERLAAWIGPADLPMRVVEGDPGIRAAGIRLGVSEAVLRPAP"
     gene            complement(4794..5381)
                     /locus_tag="VF097_05785"
     CDS             complement(4794..5381)
                     /locus_tag="VF097_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013735051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX6262342.1"
                     /translation="MMVRTGRRPGESGTREAIVEAARRAFAEHGYEGATIRGIAARAG
                     VDPALVYHFHGSKEELFAAAMRLPFQPSEVIPSLLEGEPDRLGERILRLFLKVSDQPG
                     GNPFVALMRSASTNEQAARMLREFLAREVIGRVATALGAPDAELRATFVGSQLAGLAM
                     ARYILRMEPLASADQDAVVAAVAPTLQRYLTGDLA"
     gene            complement(5378..6235)
                     /locus_tag="VF097_05790"
     CDS             complement(5378..6235)
                     /locus_tag="VF097_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012834464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6262343.1"
                     /translation="MTALAQARAVTRRFGRVVAVADAHLDLRRGEVVGLVGANGAGKT
                     TLIRVLLGLLPPTSGEVRLFGEAPSRRTRRRVGYVPQSLGLYEDLTVDENLRFAAAAY
                     GAPPPVTHDEVAGVGDWLVGELPLGLQRRVAFAAAVGHEPDLLVLDEPTSGVGPLGRA
                     RLWDDIHGAADRGVGVLVTTHYMSEAEQCHRLVVLAGGRVVAEGTLEEILGGLTVVRV
                     RTDRWEDAFARLRDAGLRLGLVGRSLRVVEARVEQVAGSLAGAGIEAEVAEAPATFEE
                     AFLMLSEKA"
     gene            complement(6232..7038)
                     /locus_tag="VF097_05795"
     CDS             complement(6232..7038)
                     /locus_tag="VF097_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012834465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6262344.1"
                     /translation="MSWGVWEISARYGDRPALEGVTLEVLPGSIASVVGADGAGKTTL
                     LRVLAGARDADAGEVRRPAPERLGFVSQGSGVYEDLTVAENLAFAARAYGVDELDGRT
                     ADLLAATGLAEARERLGSRLSGGMRQKLGVAMAMIHRPDLLVLDEPTTGLDPVSRVEL
                     WAMVAGAAAEGAAVLVSTAYLEEAERATHVLVLDEGRPLVSGSPGEVLASVPGVVLEL
                     PSRPEDVPSWRRGRTWRAWVLEGDPPPGARRVAPDIDDAVMVAALSRARS"
     gene            complement(7035..>8054)
                     /locus_tag="VF097_05800"
     CDS             complement(7035..>8054)
                     /locus_tag="VF097_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006331417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6262345.1"
                     /translation="RRTVGLMVMAPLLLLIVFGFAARFDVDEVSTIVVGEGAEAARGR
                     LPELFEVVETMPGGRAEAVQGLREGRAAAAVVVGPEPEILLDGAELFSAQAALRSLAG
                     AGGPPEGPRPRVEVLFNPGLETSAVMIPAIAGMILVFVGTLITSLGVVRERQAGTLEQ
                     LAVMPFRPWEVFLGKVSPYFVVAVFDLVLVLGVGVLLFDVPFEGAIGTFLIGSLLFLF
                     VTLGMGILVSSVSQNQGQAIQLAMMLLLPQVLLSGMIFPIESMAVGVRWIAYILPLTY
                     FIEIARGVMLRAAPLDALWPYLGLLAALGLVVFGLAVLRFRRDLAPARAGARRRPGPA
                     PEAAP"
BASE COUNT         1163 a         3083 c         2836 g          972 t
ORIGIN      
        1 gagcagggcc acctcgccga agtcgagcgc atggttgttg gccagcgtca cggcgtccac
       61 gcccaggtcc accagggtct cgaccgccgt ggccggcgcc cggaagaaga acggcttccc
      121 gggcgccggc cacggctccc cccggtccga gatgcagcac tccaggttca ggatgcagag
      181 gtcggcctcc cgcatcagcg atgccacctc tggggcgacc agggacctcg gcccgcgctc
      241 ctcgatccgc tcggccaccc cccgcccgag catcgtgtcg ccggccagcg cgatcgtgat
      301 cgccacggcc tcagcgcagg aggaagcccg tccccagggg atcgccctcc tccagcacga
      361 atcggtgctc cccgctccgg tgcgcggatc cctcgacctc ggtgaccacc gcggggaggc
      421 ggtggatctc agtctcctcc accacccggc cccggaaccg cgtccccacg atcccgtcgt
      481 gcagcatcca ctccccccgc tcgatccccc cgtcggcgtg gagcaccgcc agccgggccg
      541 aggtcccgct cccgcagggc gagcggtcca cctcgccgtc cgcgaagatc gccacgttcc
      601 gctggtggac ccgggcctcg ggttcgggag cctcctcgtg gaagatcacc ccgtacacgc
      661 cgctcagacg cccctccgcc gggtgctccg cgatcctccg ctcgttcagc gcggcctgga
      721 tctctcggcc cagggcgatg aaccgctccg tgtgctcggg ctccacggtg aggcccgcgt
      781 cagccgcggc caccgacgcg tagaaggctc ccccgtacga gacgtcggcc gtgacccccg
      841 tcacctcgat cccccgggcc ctgcagaagg acggcacgtt ccggaagcga accgaccgca
      901 cccgatcccc ctcgagccga gcccaggtcg ggagccgtcc cgaggggacg tccaccacga
      961 cccggacctc gtcgtcctcg accggcagcc cctcacgctc gagcctccca gactccagcg
     1021 cccacgtgac cagcgcgatc gtgccgtggc cgcacgcggt gctgtagccg gcgttgtgga
     1081 agaagacaac gccgaggtcg gcgccctcgt cctccgggtc cgtgacgaag cacccgtaca
     1141 tgtccgcgtg cccccggggc tcctggatca gcagttgtct gacctcgtcc agatgctccg
     1201 ccgcgaaggc tcgcttgtcg aggatcgagc ccccctcgag cgcgggagcc ccgcccgtca
     1261 cgatgcggaa gggctccccc gccgtgtggt agtcggtggc ctcgatctcc acgggcgcag
     1321 gatacgcgtg ggagactcgc ggcgtggacc ggagggagcg gtaccgggcg gctcggcgag
     1381 acccctcgct ggccgtgctg gaggggattc acgccgtgaa gcacgccatc cggttcggtg
     1441 ccgaactgct cgacgtcgtg gcctcggacg agagcccgct cgaggagctg gcggccgacc
     1501 tcgcgccgga cgcggccggt cggcttcggg ccgtcgagcg gatccccgcg gagggcttcg
     1561 cggagctctc gcccacacct ccggccacgg gcgtcgtcgc gctggcccgc cggccgtcgg
     1621 tggacatggc cgccgtgctg gccgacccgg gaccctggcc ggtgatcttc ctggaggagc
     1681 cccgccgcct ggagaacgtg ggcgccgtgg tgcgcgtcgc ggcggcggcg ggagcggccg
     1741 gcgtcctcac cactggacgg catgacccct ggcacccggc ggcgctccgc gggagcgcgg
     1801 ggctccactt cgccgtcccg gtggcccggg tggagaccct gcccgtatcc gatcgtccgc
     1861 tcgtggcgct cgacccggag gggatcgagc tgggtcccgg cgtcctcccg tcccggaccg
     1921 tggcggcgtt cgggaccgag cgggctggcc tgagtgaggg tgtcctgcgc cgagccgacc
     1981 tacgggtccg catccccatg cgggacggcg tctccagcct caacctcgcg acggccgtcg
     2041 cgatcgtcct ctacgcgcgc tgacgcgggt cagcccagga ggatccgagc gccaggaggg
     2101 cgacgatcgc gccgccgagg aggctcgtga cccgccgagc cctcgtcggg agccggtggc
     2161 ggaggaccga cccggcgccg gccagcagcc attgccagga cagggaggcg aggaaggcgc
     2221 cggcgacgaa ggcgaccttc gccgatccgg cctcgagctc cccgccgcgc agcccgagga
     2281 tcagggccgt gaagtacacg ctcgtgaccg ggttgagcag ggtgagcccg aggaacccgg
     2341 agaagatgcg gagcaggccg cctcgctccg gctcggaggc ctccgcgggc ccgagctccc
     2401 cgagcgcccg cgcgcccaac cacaccgcga tccccagcag aacgacggct ggacgaacgc
     2461 cctccccacg aacccctccc tcgacggcgg gcatcgtacc agcgacgccg cggcccccag
     2521 ccgctaccat gcgcagcagg gacgagaggg gacgcagtgg cacaggcacg gaagcagccg
     2581 ggggtcgagc ggatctaccg cgacgaccag atcgaggtca cgtgggagcc gtcgctctgc
     2641 atccactacg gggcgtgcta cggccagctc ccgagggtct tccggccccg cgagcggccg
     2701 tgggtccgcc tcgaggaggc cagcgccgac gagatcggga aggtggtcca ggactgtccg
     2761 acgggggcgc tggggttccg gcggctcgac ggcggccccc aggaggaggt gcccgagcag
     2821 gcggagatcc gagtcgatcc caagggcccg atctacctgc ggggacgggt ccggatcgag
     2881 cgcgagggcg aggtgctccg ggaggggacc cgcgtcgcgc tgtgccgctg cgggcagtcg
     2941 gggaacaagc cgttctgcga cggcacccac ctcaaggtcg gcttcaccga cgagcccgag
     3001 gcctgatcgc ccgtcggcct ccgggccatc cgcctagact ccctccatgc gcgtcggggt
     3061 ccagctcccg gaggtcgagc gcgaggtgcg ctggcccgag tatgtggcga tggcgcgagc
     3121 cgctgaggag gtgggcttcg actcgctctg ggtcggcgac cacctcctct accgggacga
     3181 cgggcggccc gagcgagggc cctgggacgc ctgggccctg ctcgcggggc tcgccgcggt
     3241 caccgagcgg gcccggctgg ggccgctcgt ggcctgcaca gcgttccggc cgccgggcgt
     3301 gctggcgcgc acggcggccg cggtccacga gctgagcggg gggcggctcg tgctcgggct
     3361 cggggcgggc tggaacgagg aggagttccg ggccttcggg attcccttcg accatcgggc
     3421 gtccaggttc gaggaggcgt tcgagatcgt ccggcgcctg ctggcgggag aaagagtgac
     3481 gttccccggg cgcttccacg aggcggagga cgcggtgctg ctgccgcggc cgtcccagag
     3541 gatcccgctc atgatcggga gcacgggccg gcgcgtgctc gccgcagcgc tcccccacgt
     3601 cgacgtgtgg aacacctggt acgactggta cggcaacacc cccgagggct tcgcccgtgg
     3661 gaacgctgag atcgatcggg ccgtggagcg ggtggggcgc gagccggagg cggtcgaacg
     3721 gagcgcgtgc gtgttcatcg tggtggacga ggaggccggc gagcgccccg cccaggaggg
     3781 cgtcacaccg ctcacgggcg gccccgagaa ggtcggcgcc ggcctccgcg agatcgcgga
     3841 ggccggcgcc gacgagctca tcctcgtcct cgatcccatc gacgagcgct cgatccgctt
     3901 ctgcggggag gcgatcgccc tggtccgagg ccggccgcct ggatagacgc cgctcgctca
     3961 gggcgccggc ctgaggaccg cctcggaaac gcccaggcgg atccccgccg cccggatgcc
     4021 cgggtctccc tcgacgacgc gcatcgggag gtcggccggg ccgatccagg ccgcgagacg
     4081 ctccgcgtcg cccgccacct ccacccacgc gaaccccttg ggctcggccg cgtgagccgc
     4141 gcgcgtccgt cccgggtaaa ggtcgtcctc gatctcccac tccatgaaga aggggaggga
     4201 cgggtccttc atcgcgacgt cgaggcccgc catccgccaa cggagcacgg ccccgtcggg
     4261 ccgctcccgg gaccaggcct cgatctcgag gcccagccgc tttgcgatca cgtcgagccc
     4321 gtcggtgcgc aggcaccagc cgaggaaccg ctcgcggccg cccaggtagt cgagcaccca
     4381 ccgcccaaag ggatgggccc gcgcctcctc ggggtcggcc accgccagca cctctacgta
     4441 gttgcggccg agcggcacga tgcggttccc ggtgcccacg ccgacatgac ggcccccttc
     4501 caccgaggcg agccccaggt ccgtccggag ccgggccgag gcggccggga ggtccacgac
     4561 gccgaggatc acgtgatcga gctcgaacat cgcgccggac gctagcacgc cccgcggcac
     4621 atcgtcgcgc tcaccctcca cgtcgatgtg gcccgagggg tcaacccttc gtgcggagcg
     4681 agtggggggc gctccacccc acgttcccgc cggcatccgc cccgccttcg gtcgaatcgc
     4741 cctcagcaga tcggtgacgg acgggacgcc gaccacttag gccggggatt tcttcaggcc
     4801 agatccccgg tgaggtagcg ctggagcgtg ggggccacgg ccgcgaccac ggcatcctgg
     4861 tcggccgagg ccagcggctc catccgcagg atgtatcgcg ccatggccaa gccagcgagc
     4921 tgggacccca cgaaggtcgc tcggagctcg gcatccggag cccccagggc cgtcgccacg
     4981 cgtccgatca cctccctcgc caggaactcg cgcagcatgc gcgccgcctg ctcgttcgtg
     5041 ctggccgacc gcatcagggc cacgaacggg tttcctcccg gctggtcgga caccttcagg
     5101 aagaggcgga ggatccgctc ccccagcctg tcgggctctc cctccagcag cgaggggatc
     5161 acctccgagg gctggaaggg gagccgcatc gcggcggcga acagctcctc cttggacccg
     5221 tggaagtggt agacgagcgc gggatcgacc ccggcccggg ccgcgatccc tcggatcgtg
     5281 gcgccctcgt agccgtgctc ggcgaaggcc cggcgcgcgg cctccacgat cgcctcgcgc
     5341 gtgccgctct ccccgggccg cctgccggtc ctgaccatca cgccttctcc gagagcatga
     5401 ggaaggcctc ctcgaaggtg gccggggcct cggcgacctc cgcctcgatc cccgccccgg
     5461 ccagggatcc ggccacctgc tccacgcggg cctcgaccac gcgcagagag cgcccgacga
     5521 gcccgaggcg aaggccggcg tcccggagcc tggcgaacgc gtcctcccat cgatcggttc
     5581 ggacgcggac cacggtgagc ccgcccagga tctcctccag ggtcccctcg gccacgaccc
     5641 gacccccggc gagcaccacg aggcgatggc actgctccgc ctcgctcatg tagtgggtcg
     5701 tgaccagcac gcccacgccg cggtcggccg ccccgtggat gtcgtcccag aggcgggccc
     5761 tccccagcgg acccacgccc gaggtcggct cgtcgaggac gagcaggtcg ggctcgtggc
     5821 cgaccgcggc tgcgaacgca acccgacgct ggaggccgag cggaagctcg cccaccagcc
     5881 agtcccccac gcccgcgacc tcgtcgtgcg tgacgggtgg cggggcgccg tacgcagccg
     5941 ccgcgaagcg gaggttctcg tccaccgtga ggtcctcgta gaggcccagg ctctgaggca
     6001 cgtagcccac gcgacgccgg gttcgccggg agggggcctc gccgaacagc cggacttcac
     6061 ccgaggtcgg ggggaggagt ccgaggagga cccggatcaa cgttgtcttc ccggccccgt
     6121 tggcgccgac gaggccgacg acctcgccgc gtcgcagatc gaggtgggcg tccgcgaccg
     6181 cgacgacccg gccgaaccga cgggtcacgg ctcgagcctg ggccagcgcc gtcacgaccg
     6241 cgccctgctc aacgcggcga ccatcaccgc gtcgtcgatg tcgggcgcga cccgacgggc
     6301 gcccggcggc gggtctccct ccaggaccca ggctcgccag gtcctgcctc tcctccacga
     6361 cgggacgtcc tccggccggc tggggagctc caggacgacg ccgggaaccg aggcgagcac
     6421 ctccccggga gagccggaca cgagcggtcg gccctcgtcg aggaccagca cgtgagtggc
     6481 ccgctcggcc tcctccaggt aggcggtgga gacgagcacc gccgccccct cggcggcggc
     6541 cccggccacc atcgcccaga gctcgacccg gctcacgggg tccaggcccg tcgtgggctc
     6601 gtccaggacg agaagatccg gccggtggat catggccatc gccaccccca gcttctgacg
     6661 catccctccg gacagccggg accccaggcg ctctcgggcc tccgccaggc cggtcgccgc
     6721 cagcaggtcg gccgtgcgac cgtcgagctc gtccacgccg taggcccggg cggcgaacgc
     6781 caggttctcc gccacggtga ggtcctcgta cacccccgag ccctgggaga cgaagcccag
     6841 ccgttcggga gccgggcgcc gcacctcccc cgcgtcggcg tcgcgggcgc cggccagcac
     6901 ccgcaggagg gtcgtctttc ccgctccgtc cgcgccgacg acggaggcga tcgaccccgg
     6961 caggacctcc agggtcacgc cctcgagcgc cggacggtcc ccgtagcggg ctgagatctc
     7021 ccagacgccc cagctcatgg cgcggcctcg ggggccggcc cgggccggcg ccgggcgccg
     7081 gcccgggccg gcgccagatc ccgccggaac cggaggacgg cgagcccgaa caccaccagg
     7141 ccgagcgcgg ccagcagccc caggtacggc cacagggcat cgagcggagc ggcgcgcagc
     7201 atcacgcccc tcgcgatctc gatgaagtag gtcagcggca ggatgtaggc gatccaccgc
     7261 acccccaccg ccatcgactc gatcgggaag atcatcccgg agaggaggac ctgggggagc
     7321 aggagcatca tcgccagctg gatcgcctgc ccctggttct gactgacgct cgacaccagg
     7381 atgcccatgc ccagcgtcac gaacaggaac agcagggacc cgatcaggaa cgtcccgatc
     7441 gcaccctcga acgggacgtc gaagagcagc acccccaccc ccaggacgag cacgaggtcg
     7501 aagaccgcca ccacgaagta gggcgacacc ttgcccagga agacctccca cggccggaag
     7561 ggcatcacgg ccagctgctc gagggtaccg gcctgacgct cgcgcaccac gcccaggctc
     7621 gtgatcaggg tgcccacgaa gacgaggatc atcccggcga tggcggggat catgacggcc
     7681 gacgtctcga gccctgggtt gaagaggacc tccactcggg ggcgcgggcc ctcgggcgga
     7741 ccgccggcgc ctgccaggct ccgcagcgcc gcctgggcgg agaagagctc ggccccgtcc
     7801 agcaagatct cgggctccgg cccgacgacc acggctgcgg cggccctgcc ctcacgcagg
     7861 ccttggacgg cctcggcccg gccgccgggc atcgtctcca ccacctcgaa cagctcgggg
     7921 aggcgccccc gcgccgcctc ggcgccctcc cccacgacga tcgtggagac ctcgtcgacg
     7981 tcgaacctcg ccgcgaagcc gaagacgatc agcaggagca gcggcgccat caccatcagc
     8041 cccacggtcc gccg
//
LOCUS       DASUTZ010000130        11409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1590006, whole genome shotgun sequence.
ACCESSION   DASUTZ010000130 DASUTZ010000000
VERSION     DASUTZ010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11409
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590006"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1898)
                     /locus_tag="VF097_05805"
     CDS             complement(111..1898)
                     /locus_tag="VF097_05805"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEX6262346.1"
                     /translation="MRSRLTRGRVAAVAVAVLMVPLVGASASAEPGTTTLIASSHRVT
                     YGETLSLQGVLSTADACLAGRSVALQRRLPGMVGWEEVTVASTGAGGAFAFQTVPQEI
                     ADYRALIAPETREAVPCDEVLSQVVGIEVEAAVSLALSRNPVPASGCVRLRIAVLPTK
                     PGHEVVLQRRRGGSWATEETLILGADSQASRHTCMTWSEIGPHRFRARWPRQDEANFA
                     AASSPVRMQVVKAAWMKRIDAIAAPLRMGVSVREQGAFLFRRGDTGLRTPASNQKLLL
                     SMALLDALGPDRRLATKAVSRRPRNGVVRGNLWIVGSGDPEVGPSKVRKLAARIEEAG
                     VRRITGGVRGSTALFARDWWAPGWRSYFPRTVIALPTALTFRGNVVGGHHVRDPERRA
                     ALALTKQLRRRGIRVVKPPGMGRHPSGRPELTRVESRHLSGILAITNHASSNFWAEVL
                     TKGLAVARRKPPGTIAKGAGAIAAWVKANGGRSRSHDGSGLSYANRTTPASIAKLLGR
                     AEGEAWGDRLRSSLPAGGVGTLSRRLHDVPVRAKTGTLSGISALSGWVESKRTGTWVE
                     FSLLSQGVSTTRAKAAEDRIVRILWRNAR"
     gene            1975..3207
                     /locus_tag="VF097_05810"
     CDS             1975..3207
                     /locus_tag="VF097_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015195388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HEX6262347.1"
                     /translation="MGLDVEAARKRFPALERTIEGRPCVFADAPAGTQVPEVVIDAMA
                     DYLRRSNANVGGVFATSLETDEAIAAARRSAADLLGCDPREVVFGPNMTTLSLALSRA
                     VARELGPGDELVVTVLDHDANVAPWLLAAEDRGATVRWANLDEVDCTLDLASLERALG
                     GRTRVVALTLASNAVGSLTPVDEVVAMVRERAPEAILVGDAVHLAPHRSIDAGPFDAV
                     LCSPYKFFGPHLGIMAARRGLLERLRPYKVRPAPEEPPDRWETGTQSHEALAGLTAAI
                     SYLRDLVEGEGRRGVLAAMEEIREHESLLSRRFLEGITRIPAVRLYGIGDPERVDQRT
                     PTFAIRVGGQHPRSTAEKLARRGIFVWDGNYYALAIMERLGLENSGGAVRIGFCHYHT
                     EDEVDRVLEEVAALAAAP"
     gene            3209..3991
                     /gene="fabI"
                     /locus_tag="VF097_05815"
     CDS             3209..3991
                     /gene="fabI"
                     /locus_tag="VF097_05815"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEX6262348.1"
                     /translation="MPTMLEGKRLLFTGVLTPQSIAFHCAKQVQELGAEVLLTGFGRG
                     MSITEKSAKRLPEPADVLEMDVNDPEHVAGVAAAVGHRWERLDGLLHAIAFAPQDALG
                     GRFLETEWPSVATALQTSAFSYKALVTAFLPLLRGGGSAVTLDFDASVAWPGYDWMGV
                     AKAALEAVTRYLARDVGREGVRVNAVSAGPLKTVAAKGIPGFDVFTDLWHKRAPLGWD
                     VKDPSPVAGAVAFLLSDHSRGITGEILHVDGGYHAMGADLQE"
     gene            4032..5111
                     /locus_tag="VF097_05820"
     CDS             4032..5111
                     /locus_tag="VF097_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX6262349.1"
                     /translation="MSTRARTAALLLAGSLLITACAGGPGITIEAPEQAAGTAASPSP
                     IPPLDTDEPIPAVVERVSPAVVNVRVNQAGGATGTGTGFVVRRNGIIVTNFHVVEGAF
                     SIQVTLADGREFEARAIGGDPNADLAILQIEAQQLPTVELGDSDALRLGETVVALGFA
                     LALEGGPSVTSGIVSAKGRSIETQDSRGAQNTLEELIQTDAPINPGNSGGPLVDLAGR
                     VIGINTAGVQASEAENIGFAIAINRARPIIEDAIASPEAEAAYLGVSTQDVTPVVAAQ
                     LDLPVDEGALVRGVAPGTGAEEAGIVVGDVIVEIEGQAIGSSEDLGSAIREHAPGDEI
                     SITVIRDGGARTVTAELGVRPLPTG"
     gene            5108..5893
                     /locus_tag="VF097_05825"
     CDS             5108..5893
                     /locus_tag="VF097_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HEX6262350.1"
                     /translation="MSDPLAAGERALLVDARGRRFLITLEEGGEFHFHGGVVAHDDVI
                     GRPEGSRIESRSGSALTAFRPRLADFVLKMSRGAQVVYPKDLGAILIQADVYPGARVL
                     EAGTGSAALTIALCRTTGPEGRVVSYELREDHREVAERNVESFFGRVPAWLELRAGDV
                     RQAAETEERFDRAVLDLPEPWEALPEVTNALDPGAILCGYLPTTPQVQSLVVAMLDRG
                     YREVEAFEVLQRGWHVTARSVRPDHRMVAHTGFLVVGRRSADA"
     gene            5961..7634
                     /gene="arc"
                     /locus_tag="VF097_05830"
                     /pseudo
     CDS             5961..7634
                     /gene="arc"
                     /locus_tag="VF097_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019609145.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
     gene            8005..10707
                     /locus_tag="VF097_05835"
     CDS             8005..10707
                     /locus_tag="VF097_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013733552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HEX6262351.1"
                     /translation="MAATIAMIALMFGPVPGTSVAQNGVTVDDAIDIAFDHVQGDPEA
                     LGVTAEDVAELAVASAYRSDHNQVVHVNLRQFHEGDPVGNSHVTVNVAGDGTVLFVGH
                     NLESVSEDEGDVAFGPVAAVRAAADALGLDPDGLRVLGRGGASVDAVVSGGGISDQPI
                     PAGLIWEPTQDGLRKAWDLVIDDATTASLWSVAVDAETGELLRQDDWTDHDTLAGLAS
                     GLARFEYSPGDTTFLEPINSVDDGSSYRVFQAPTESPLDHDRRLVENPAEATASPFGW
                     HDTDGVPGPEFTITRGNNVHAYTDHDNSNDPDEGKTVVIVDPPSSAAGEYVAVQASFG
                     PDIPMGGVSGAIELGDDGMGTNVNDGCEPYTVSAGAIALVDRGQCNFTVKVLNGQNGG
                     AAAVIVANNQPTPPFAMGGNDPAITIPSVMISMDDGNTVKAGLPATGSMQVIPLPTPS
                     APDGGAGLDFDYPIDLTTEHPHDYWDAAVTNLFYMNNVIHDVMYRLGFNEVAGNFQVT
                     NYNTGLGNGGDDVRAEAQDGGGTNNANFATPAMDGGRPRMQMYLWTLGTPVRDGDFEN
                     GIIIHEYGHGISNRLTGGPTVNCLNGDERMGEGWSDYYAITMLADPTLDDPDGPRGMG
                     PYALFQDSRQDGGIRPRPYSRDMNIQPFTYDRIKTGAWITGGSLATPHGVGHAWAATL
                     WDMTWNLIDRHGFNPNIYEPSGTGGNNLALQLVTDGLKFQGCAPGFVVGRDAILAADQ
                     ALTGGENACIIWASFARRGLGFSAVQGTTDRDDNAEAFDTHPACAAPGTGFSPPVSNT
                     TLNTFDAGETVPLMFNIGEDRGPNPLASNSPASQEIDCTTRTAVQYAITTNTDSTNGK
                     LTFNKNLQRYHYNWATSADWAGTCRQVIITLDDGTQLRADFRFE"
     gene            10821..11360
                     /locus_tag="VF097_05840"
     CDS             10821..11360
                     /locus_tag="VF097_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262352.1"
                     /translation="MWIVIVVAAVLVIAAIAWAVWERRRREGLRDRYGPEYERLEEDE
                     GARHAAAELRERERRRETFDIRPLSSEQRSRFADAWEDLQARFVDAPNVAVLEADRLV
                     IEVMRAKGYPMDDFDQRAADLSVDHPRVVENYRTAHDVAMRSEKGDADTEELRKATLA
                     YRELFEELLEEEPGYERTG"
BASE COUNT         1846 a         3939 c         4064 g         1560 t
ORIGIN      
        1 gcgcccgccg ccgtgtgggc gggcgcaacc gcggccgacg cccgtcgagt gatctgtatc
       61 ggcaccggct ccaacatcga cccggacctg ctcgacgact gccttcgggg ctagcgagca
      121 ttccgccaga ggatgcgcac gatgcggtcc tcggcggcct tggcgcgggt agtggacacg
      181 ccctgcgaca gcagggagaa ctcgacccag gtgcccgtgc gcttcgactc cacccagccc
      241 gagagggcgc tgatgccgct cagcgtcccc gtcttggcgc gcaccgggac atcgtggagc
      301 cggcgggaga gcgtgccgac ccctccggcg gggaggctcg agcgcaggcg atcgccccag
      361 gcctcgccct cggcccgacc gagcagcttg gcgatcgacg ccggggtcgt gcggttcgcg
      421 tacgagagcc ccgagccgtc gtggctgcgg gaacgcccgc cgttcgcctt cacccacgcc
      481 gcgatcgcgc cggccccctt ggcgatcgtg ccgggcggct tgcgccgggc cacggccagg
      541 cccttcgtca ggacctcggc ccagaagttc gaggacgcgt ggttcgtgat cgcgaggatg
      601 cccgagaggt gacgcgactc gacccgtgtt agctcgggcc ggccggaggg gtgccgcccc
      661 atccccggcg gcttcaccac gcggatgccg cgtctcctga gctgcttggt gagggccagc
      721 gcggctctgc gctcagggtc gcgcacgtga tgtccgccga ccacgtttcc gcggaacgtg
      781 agtgccgtgg ggagcgcgat caccgtgcgc gggaagtagg agcgccagcc cggcgcccac
      841 cagtcgcggg cgaagagcgc ggtgctgccc cggacgcccc cagtgatcct ccgaacgccc
      901 gcctcctcga tgcgcgcggc gagcttccgc accttcgagg gaccgacctc cggatcgccg
      961 gaccccacga tccagaggtt tccccggacc accccgttcc ggggccggcg ggacaccgcc
     1021 ttcgtggcga gcctccgatc cgggcccagc gcgtccagga gcgccatcga gagcagcagc
     1081 ttctggttcg acgcgggggt gcggaggccc gtgtcccccc gccggaagag gaaggcgccc
     1141 tgctcgcgca ccgacacgcc catccggagg ggcgccgcta tcgcgtcgat cctcttcatc
     1201 cacgccgcct tcaccacctg catgcgaacg ggggaagagg ctgccgcgaa gttcgcctcg
     1261 tcctggcggg gccaccgcgc tcggaaccgg tggggcccga tctcgctcca ggtcatgcag
     1321 gtgtggcggg aagcctgaga gtccgccccc aggatcaggg tctcctcggt agcccaggag
     1381 cctccgcgac ggcgctggag caccacctcg tgtcccggct tggtcgggag cacggcgatc
     1441 cggagccgga cgcagccgga ggcggggacc gggttcctgg agagagccag gctgaccgcg
     1501 gcctccacct cgatccccac cacctgcgag aggacctcgt cgcaggggac ggcctcccgc
     1561 gtctcggggg cgatcagggc tcggtagtcc gcgatctcct ggggcacggt ctggaaggcg
     1621 aaggcccctc ccgctcccgt cgaggcgacc gtgacctcct cccagcccac catgcccggc
     1681 agacgtcgct ggagggccac cgagcggccc gcgaggcagg cgtcggcggt ggagaggacg
     1741 ccctgcagcg acagcgtctc gccgtaggtc acgcggtgag aggaagcgat cagcgtcgtc
     1801 gtgcccggct cggccgacgc tgacgcgccg acgaggggca ccatcagcac ggcgacggcc
     1861 accgccgcga cccgaccgcg ggtgaggcga gagcgcaccg gcccattttg gcggccaggg
     1921 ccggctcgtc aagacgggtg gcggcgcccg gggggatgcg ccaccctgtc gcccatgggc
     1981 ctggacgtgg aggcggcgcg gaagcgattc cccgccctcg agcggacgat cgagggtcgc
     2041 ccgtgcgtct tcgccgacgc gcccgcaggg acccaggtgc ccgaagtagt gatcgacgcg
     2101 atggccgact acctgcgccg gagcaacgcc aacgtcggcg gggtcttcgc cacgagcctc
     2161 gagaccgacg aggcgatcgc cgccgccagg cgatccgccg ccgacctgct gggctgcgac
     2221 ccgcgagagg tggtgttcgg cccgaacatg accaccctga gcctggccct ctcgcgggcg
     2281 gtggctcgag agctcggacc gggcgacgag ctcgtggtga ccgtcctcga tcacgacgcg
     2341 aacgtggccc catggctcct ggccgccgag gacaggggag cgaccgtccg atgggcgaac
     2401 ctcgacgagg tggactgcac gctcgatctg gcgagcctgg agcgagccct gggcgggcgc
     2461 acgcgcgtcg tcgcgctgac gctcgcttca aacgccgtgg gaagcctcac cccggtggac
     2521 gaggtggtcg cgatggtccg ggagcgagcg ccggaagcga tcctcgtggg ggacgcggtc
     2581 cacctggcgc cccaccgctc gatcgacgcg ggacccttcg acgcggtgct ctgctccccg
     2641 tacaagttct tcggtcccca cctagggatc atggcggcga ggagagggct gctggaacga
     2701 ctccggccgt acaaggtccg gccggctccc gaagagccgc ccgaccgctg ggagaccggc
     2761 acccagagtc acgaggccct ggccggtctg acggccgcga tcagctacct ccgcgatctc
     2821 gtggagggag agggccgccg tggcgtcctg gccgcgatgg aggagatccg ggagcacgag
     2881 agcctcctct ccagacgttt cctcgagggg atcactcgaa tccccgccgt gcggctctac
     2941 ggcatcggcg accccgagcg cgtggaccag cgcaccccga cgttcgcgat ccgggtgggc
     3001 ggccagcacc cccggtccac cgccgagaag ctggcccgac gggggatctt cgtctgggac
     3061 ggcaactact acgcgctggc gatcatggag cggctcgggc tggagaactc gggcggggcg
     3121 gtccggatcg gcttctgcca ctaccacacc gaggacgagg tggaccgcgt gttggaggag
     3181 gtggcggctc tggccgccgc tccataggat gcccacgatg ctcgagggca agcggctgct
     3241 gttcaccggg gtgctgacac cccaaagcat cgccttccac tgcgccaagc aagtccagga
     3301 gctgggcgcc gaggtgctgc tcaccggctt cggtcgcggg atgtcgatca ccgagaagag
     3361 cgcgaagcgc ctccccgagc ccgccgacgt cctggagatg gacgtgaacg atccggagca
     3421 cgtagcgggc gtggcggcgg cggtggggca ccgctgggag cgcctggacg gcctcctcca
     3481 cgcgatcgcc ttcgcacccc aggacgccct tggagggaga ttcctcgaga cggagtggcc
     3541 cagcgtggcc accgccctac agacgagcgc cttctcgtac aaggctctgg tcacggcgtt
     3601 cctacccctg ctcagggggg gtggcagcgc cgtgaccctg gacttcgacg cctcggtggc
     3661 ctggcccggc tacgactgga tgggggtggc gaaggccgcg ctcgaggccg tcacccggta
     3721 cctcgcccgc gacgtgggtc gcgagggcgt ccgcgtcaac gcggtgtcgg cggggccgct
     3781 caagaccgtc gcggccaagg ggatccccgg cttcgatgtc ttcacggacc tctggcataa
     3841 gcgcgcgccc ctcgggtggg acgtgaagga tccgtcgccg gtggccggag ccgtggcctt
     3901 cctgctgagc gaccactcgc gggggatcac cggcgagatc ctccacgtgg acggcggcta
     3961 ccacgcgatg ggggccgacc tgcaggaata gcgcccgggc cttcgccggg cgcgatgcta
     4021 tcctcggcga gatgagcacg cgggccagga cggccgccct gctgctcgcc ggctccctac
     4081 tgatcacggc gtgcgcaggc ggtcccggga tcaccatcga ggctcccgag caagcggccg
     4141 ggaccgcggc atccccatcc ccgatcccac cgctcgatac cgatgagccg atcccggccg
     4201 tggtggagcg cgtcagcccg gccgtggtga acgtccgggt gaatcaggcg gggggcgcca
     4261 cggggaccgg cacgggcttc gtcgtccgtc ggaacgggat tatcgtcacg aacttccacg
     4321 tcgtggaagg tgccttcagc atccaagtga ccctggccga cgggagggag ttcgaggcgc
     4381 gagcgatcgg gggcgacccc aacgccgacc tcgcgatcct ccagatcgag gctcagcagc
     4441 tgcccaccgt cgagctcggg gactccgacg ccctgcgctt gggtgagacg gtcgtcgcgc
     4501 tggggttcgc cctggccctg gagggtgggc cctccgtgac ctcggggatc gtgtccgcga
     4561 agggccggtc gatcgagacc caggacagcc ggggcgcgca gaacaccctc gaggagctga
     4621 tccagaccga cgccccgatc aacccgggga actccggggg gccgctcgtg gacctggccg
     4681 gccgcgtgat cggcatcaac accgccgggg tccaggcatc cgaggccgag aacatcggct
     4741 tcgccatcgc catcaaccgg gccaggccca tcatcgagga tgcgatcgcc agccccgagg
     4801 ccgaggccgc ctacctcggg gtctcgaccc aggacgtcac cccggtcgtc gctgcacagc
     4861 tcgacctccc cgtcgacgag ggtgccctcg tgcggggggt cgcgcccggg accggtgccg
     4921 aggaggccgg catcgtggtc ggagacgtga tcgtcgagat cgagggccag gcgatcggga
     4981 gcagcgagga cctgggcagc gcgatccggg agcacgcccc gggcgacgag atctcgatca
     5041 cggtcatccg agacggaggg gcccgaaccg tcacggccga gctgggggtc aggcccctcc
     5101 cgaccggatg agcgacccgc tggcggcggg ggagcgagcg ctcctcgtgg acgcccgcgg
     5161 gcgcaggttc ctcatcaccc tggaggaggg tggcgagttt cacttccacg ggggcgtggt
     5221 ggcgcacgac gacgtgatcg ggaggcccga gggctcccgg atcgagtcca ggagcgggag
     5281 cgcgctgacc gcgttccgcc ctcggctcgc ggacttcgtt ctgaagatgt cgcgtggggc
     5341 acaggtggtc tacccgaagg atctcggtgc catcctgatc caggcggacg tctacccggg
     5401 cgcgcgggtc ctcgaggccg gcacgggatc ggccgccctg acgatcgcgc tctgccggac
     5461 gaccgggccg gagggccggg tcgtctccta cgagctccgg gaggaccacc gagaggtcgc
     5521 cgaacggaac gtcgagtcgt tcttcggccg ggtccccgcc tggctcgagc tccgcgccgg
     5581 tgacgtgcgg caggcggcag agactgagga gcgcttcgac cgcgcggtcc tcgacctccc
     5641 cgagccgtgg gaggcgctcc ccgaggtcac gaacgcgctg gacccgggcg cgatcctgtg
     5701 cggctacctg cccaccacgc cgcaggtgca gtcgctcgtc gtggccatgc tcgaccgcgg
     5761 ctaccgcgag gtcgaggcct tcgaggtgct ccagcggggc tggcacgtga cggctcgcag
     5821 cgtccgcccc gaccaccgga tggtggccca caccggcttc ctggtggtgg ggcgccgcag
     5881 cgcggacgct tgagcgccct tcgatcaccc atcgaggggc cgccgctata cttgggcctc
     5941 gaggggagga cccgcacccg atgcagccgg accgcgacgt cgagaaagag atccacgagc
     6001 tccagaccca ggtgcgattc ctggaggacg agctggccgt gctccggcag cgccagcact
     6061 ccacgccgcg tcaggtgaag gtgctcgagg agcgcctgct ggagtcgaag tcccaggtcg
     6121 ccaaggcgct ggcccagaac gaaaagctcg tctccgccct caagaccgag cgggagcgca
     6181 tcgaggccct caaggaggag gtcgataagc tcacccggcc ccccgcctcc ttcggcgtgt
     6241 tcgtccgcga gaacgaggac ggctccgccg acgtcttcac gtccggccgc aagatgcggg
     6301 tcaatgtcag cccggaagtg gatcgctccc tcctgcaccg cggcgcagag gtcatcctca
     6361 acgaggccct caacgtggtg gaggtgctgg agcaggaccg ccagggcgag gtcgtcagcg
     6421 tgaaggagat cctgggcgac ggccgcgtga tcgtgctggg ccgcggcgac gaggagatgg
     6481 tggcgatcgt ctccaagcgc ctccagggcg agcatttccg ggccggcgac gccctccttt
     6541 acgatcctcg gtccgggcac gtgctggagc gcctcccgaa ggaggagatc gaggagctcg
     6601 tcctggagga ggtgcccgac gtcacctacg acgacatcgg cggcctcgcc ctccagatcg
     6661 aggacatccg cgacgcggtg gagatgccgt tcctgtacgc cgagctgttc cgcgagcatc
     6721 ggctgcgtcc cccgaagggc gtcctcctct acggcccgcc gggctgcggg aagaccctga
     6781 tcgccaaggc ggtcgcaaac tcgctcgcca agcaggtggc cgagaagacc ggccgccccg
     6841 aggcccactc gtacttcctc aacgtcaagg gaccagagct gctcaacaag tacgtcggcg
     6901 agaccgagcg gcagatccgc gtgatcttcc agcgggcgaa ggagaaggct caggagggct
     6961 tccccgtgat cgtgttcttc gacgagatgg actcgatctt ccggacgcga gggacgggga
     7021 tctcctcgga cgtggagaac acgatcgtgc cccagctgct ggccgagatc gacggggtcg
     7081 agaccctgaa gaacgtcatc gtgatcggcg cctcgaaccg agaagacatg atcgacccgg
     7141 cgatcctccg cccggggcgc ctcgacgtga agatcaagat cgagcgtccc gacatcgagg
     7201 ccgcgcgcaa catcatgtcg aagtacctca cgctcgacgt cccgatccac cccgacgagt
     7261 tgaagggcta cgacggcgac aaggcgcagg ccctgcccgc gatgatcgac cgcacgatcg
     7321 atgcgatgta cgccgagacc ggggagaacc gcttcctcga ggtgacctac caaaacggcg
     7381 acaaggagat cctctacttc aaggacttca actccggggc gatgatcgag aacatcgtgg
     7441 cccgggccaa gaagtcggcc atcaagcagt tcctccagat cggcgagaag gggatcaaga
     7501 tccagcacct gctcgacgcc atccgagcgg agttcaagga gcacgaggat ctgcccaaca
     7561 cgacgaaccc cgacgactgg gcgcggatct ccgggaagaa gggtgagcgg atcgtctacg
     7621 tccgcaccct gctgggcgag gccgacggct cgggtagccg ccaggtcgac cggatccagc
     7681 ccggccagta cctctgagca gagcgaacgc tgagcgcgtc cgtcgcctga tttgactggc
     7741 ctccgtccga ttccgtaggt ttgctccgac ggctacaggc cgtcggtcgg tgcccgcact
     7801 ccgatcgctg gaggattctg gacggatgcg acggaacggg cgccgcgctc atggctcgtt
     7861 cgcgccgcgt tcgcggggac cccctccctc cggcactccc acgtcacgtg ttccgctgcg
     7921 gctgcggtga gcccgcctgc ggccgccggt cgcccctcgg ggcggaggga ggtaaggatg
     7981 tcgcatagga gaagggtcac gatcatggcg gcgacgatcg ccatgatcgc gctcatgttc
     8041 ggaccggtac ccgggacgtc ggtcgcccag aacggtgtca ccgtcgacga cgcgatcgac
     8101 atcgcgttcg accacgtcca gggtgatccg gaggccctcg gcgtcaccgc ggaggacgtg
     8161 gccgagctcg ccgtggccag cgcctatcgg agcgaccaca accaggtggt ccacgtcaac
     8221 ctgcgccagt tccacgaggg ggacccggtg ggcaactcgc acgtcaccgt gaacgtggcg
     8281 ggcgacggca cggtgctctt cgtcggccac aacctcgaat cggtttcaga ggacgagggc
     8341 gacgtcgcgt tcggcccggt ggcggccgtc cgagccgccg ccgacgcgct cggcctcgat
     8401 cccgatggcc ttcgggtcct gggccgcggt ggcgcctccg tggacgccgt ggtgtccggg
     8461 ggcgggatct ctgatcagcc gatccccgcg ggcctgatct gggagccaac gcaggacggg
     8521 ctcaggaagg cctgggacct cgtcatcgac gacgcgacca ccgcgagcct ctggagcgtg
     8581 gcggtcgacg cggagaccgg ggagctgctg cgccaggatg actggacgga tcacgacacc
     8641 ctggcgggcc tggccagcgg actggcgcgg ttcgaatact cgcccggcga cacgacgttc
     8701 ctcgagccga tcaactcggt ggacgacggc tcgagctacc gagtgttcca ggcgccgacc
     8761 gagagcccgc tggatcacga ccgtcgcctg gtggagaacc cggccgaggc gaccgcctcg
     8821 cctttcggct ggcacgacac cgacggcgtt cccggtcccg aattcacgat cacgcgcggg
     8881 aacaacgtcc atgcgtacac cgaccacgac aacagcaacg acccggacga gggcaagacg
     8941 gtggttatcg tcgacccgcc atcctcggcg gccggcgagt acgtcgcggt gcaggcgtcc
     9001 ttcgggccgg acatcccgat gggcggcgtc tcgggagcca tcgagctcgg ggacgacggc
     9061 atggggacga acgtgaacga cggctgtgag ccctacaccg tctccgccgg cgcgatcgcg
     9121 ctcgtggacc ggggccagtg caacttcacc gtgaaggtcc tgaacggcca gaacggcggc
     9181 gccgccgcgg tgatcgtggc caacaaccag cccacgcctc cgttcgcgat gggcggcaac
     9241 gacccggcga tcacgatccc gtcggtgatg atctcgatgg acgacggaaa cacggtgaag
     9301 gcgggactcc cggcgacggg cagcatgcag gtgatccccc tcccgacccc ctcggcgccg
     9361 gacggtggag cgggcctgga cttcgactac ccgatcgacc tgacgaccga gcatccgcac
     9421 gactactggg atgctgcggt cacgaacctc ttctacatga acaacgtcat ccacgacgtc
     9481 atgtaccggc tggggttcaa cgaggtcgcc gggaacttcc aggtcacgaa ctacaacacc
     9541 ggactgggga atggcggcga cgacgtccgg gcggaggccc aggacggcgg cggcaccaac
     9601 aacgccaact tcgccacgcc ggcgatggac ggcggccgac ccaggatgca gatgtacctg
     9661 tggacgctcg gcacgccggt ccgagacggc gacttcgaga acggcatcat catccacgag
     9721 tacggccacg ggatctccaa ccggctcacg ggagggccca cggtcaactg cctgaacggc
     9781 gacgagcgca tgggtgaggg ctggagcgac tactacgcca tcaccatgct ggccgatccg
     9841 accctggacg acccggacgg gccgcggggg atggggccgt acgcgctgtt ccaggacagc
     9901 cgccaggacg ggggcatccg tccgcgtccg tactccaggg acatgaacat ccagccgttc
     9961 acctatgacc ggatcaagac cggtgcctgg atcaccggtg ggtcgctggc cactccgcat
    10021 ggggtgggcc acgcgtgggc ggccaccctg tgggatatga cctggaacct gatcgaccgt
    10081 cacggcttca acccgaacat ctacgagccc tcggggacgg gcggcaacaa cttggcgctg
    10141 cagctcgtga cggacgggct gaaattccag gggtgcgccc ccgggttcgt ggtcgggcgt
    10201 gacgcgatcc tggccgcgga ccaggccctc accggcggcg agaacgcctg catcatctgg
    10261 gcgtcgttcg cgcgaagggg actggggttc agcgcggtcc agggcaccac ggaccgggac
    10321 gacaacgccg aggcgttcga cacgcacccc gcgtgcgcag cgcccgggac cgggttctcg
    10381 ccaccggtat cgaacaccac attgaacacg ttcgatgccg gagagacggt cccgctgatg
    10441 ttcaacatcg gtgaggaccg cggcccgaac ccgctggcga gcaactcgcc ggcgtcgcag
    10501 gagatcgact gcaccacgcg gaccgcggtg cagtatgcga tcaccacgaa caccgactcc
    10561 acgaacggga agctgacgtt taacaagaac ctccagcgct accactacaa ctgggcaacg
    10621 tccgcggact gggcagggac ctgccgccag gtgatcatca ccctggacga cgggacccag
    10681 ctccgagcgg acttccggtt cgagtaggag tcgcggcatg agggaggggg ccgcccggcc
    10741 ccctccctcc tctgctccgg tttcacccgt ccagccaccg ggtacgagcc gcccgggggt
    10801 gaagagacat ggatgcatgg gtgtggatcg tgatcgtggt cgcggcggtg ctggtgatcg
    10861 cggcgatcgc gtgggccgtc tgggagcgcc gccgccgcga ggggctccgc gatcgatacg
    10921 gccccgagta cgagcggctc gaggaagatg agggagcgcg gcacgcggcg gccgagctgc
    10981 gcgagcggga gcgtcgacgg gagaccttcg acatccgtcc cctgagctcc gagcagcggt
    11041 cgcgcttcgc cgacgcgtgg gaggatctcc aggcgcggtt cgtggacgcc ccgaacgtcg
    11101 cggtgctgga ggcggaccgg ctggtgatcg aggtcatgcg ggcgaagggc tacccgatgg
    11161 atgacttcga ccagcgggcg gccgacctgt cggtcgacca tccgcgggtg gtcgagaact
    11221 accggacggc gcacgacgtc gccatgcgtt ccgagaaggg cgacgcggac accgaggagc
    11281 tccgcaaggc gacgctggcc taccgggagc tcttcgagga gctgctcgag gaggagcccg
    11341 ggtacgagcg gaccggctga cgtcggcccc gaacgggcgc cgggatgcgc ggaggcccgg
    11401 cgggcctcc
//
LOCUS       DASUTZ010000131         6473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_942968, whole genome shotgun sequence.
ACCESSION   DASUTZ010000131 DASUTZ010000000
VERSION     DASUTZ010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6473
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942968"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="VF097_05845"
     CDS             <1..746
                     /locus_tag="VF097_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CpaF/VirB11 family protein"
                     /protein_id="HEX6262353.1"
                     /translation="APSPYGPEDLLRLGTLEERTLEVLETCVRGRANILISGGAGSGK
                     TTLLGVLSSFIPAGERIVTIEDAAELRLRSAHVVRLEARPASAEGRGAVTVRDLVRNA
                     LRMRPDRIVVGEVRGGEALDMLQAMNTGHEGSMSTVHANSPQELMARLETMALMADVV
                     LPADHLREHIAQTVDLVVHMVRLREGRRILARLTSVEGPAGGRPLLRDVFRYAGGGWE
                     AVGSPGGIALRQGRDELDLLGFSPNGTAR"
     gene            743..1549
                     /locus_tag="VF097_05850"
     CDS             743..1549
                     /locus_tag="VF097_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX6262354.1"
                     /translation="MIEVGGPGVMGLATGASILAAAAERRRMSRRLEAPVDPTGGRAA
                     ALLGAGLCSVAAGLLLFGPLASLLACGGLVGWKLIHHARAREAEALETERGLRDAVRA
                     LAASARVGLSIRRSLEEVAREGPPAVAGPFRRAARELAMGRPLPAALAGLSDRLGSRE
                     AAMLTGVLSIHHRAGGDLPTALDEVAALAERRAEARRRVRALTAQGRASGAVLALLPV
                     AFVGLLSGTSGNGLGTIYATPRGAALLGAGLALQLVGYLWIRAILRRAQP"
     gene            1546..2358
                     /locus_tag="VF097_05855"
     CDS             1546..2358
                     /locus_tag="VF097_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX6262355.1"
                     /translation="MIVVGLGYAAAAALLAAAAARRSPELARLGVPPRPRRDPLEALG
                     RRVPWTPPVLAERLEILGRPAEVDRLRGAAAVCALVGLIGGSLTSAPSPAAVGVGALA
                     AAAGWRLPAFTLARRAASARGQMLPEVASLLDLVTVGLSAGLTPRLAIDRAVDHVAEP
                     LREALVRIRREVALGASWPSSLDHLGRRLALPDLGRLASTLDRSSRLGAPAADPIRSL
                     ARDVRAERYAEVEARARRAPVTMLFPLVLCILPAFVLAAVLPAMLVATRGVP"
     gene            2368..2592
                     /locus_tag="VF097_05860"
     CDS             2368..2592
                     /locus_tag="VF097_05860"
                     /inference="COORDINATES: protein motif:HMM:NF025395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4244 domain-containing protein"
                     /protein_id="HEX6262356.1"
                     /translation="MIRIWTALVSWWIVLRARLLDRLSGSKGQTTAEYALVILAAAAI
                     AIGLLAWARSSDRLPAFFDRIIDRILGEAE"
     gene            2517..2951
                     /locus_tag="VF097_05865"
     CDS             2517..2951
                     /locus_tag="VF097_05865"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HEX6262357.1"
                     /translation="MGPVLRSPPGVLRPHHRSDPRRGGVAGSAAVEFALVLPLVALVI
                     AGLVQVVRLGGESLSVLSAAREGARTAAVTIDDGEVRRAVIASGLDEARADILVERGG
                     GVGTAVTVSLSYRAPAGPPILSWLLPTEVTLRARATMRQEVG"
     gene            2948..3595
                     /locus_tag="VF097_05870"
     CDS             2948..3595
                     /locus_tag="VF097_05870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3654c family TadE-like protein"
                     /protein_id="HEX6262358.1"
                     /translation="MRERGSVTIVVAVAMGMAAISAALMGDLGAVATGAARAQAAADA
                     AALAAAQELVLPSHESPVEAAERYAALNGARLIRCRCPPGGEDALVLVERSVRLPFLG
                     GTRTLRRAARATVALEGDVAGLEPWFLSRLACLRARVPDLWVVSGFRTRAEQTRLHEE
                     KPALAAPPGRSLHELGLAADLGFGSASAGTLAHQQAPACGLVFPLSHEPWHVEPA"
     gene            complement(3592..4419)
                     /locus_tag="VF097_05875"
     CDS             complement(3592..4419)
                     /locus_tag="VF097_05875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6262359.1"
                     /translation="MATFGLFLPHREDPVAAARHAEELGLDMVTISDHLHGARTWEPW
                     TVLTWIAASTSRVRVSPNVLGLPYRHPAVTAKMVETLHRLSEGRVVLGIGAGGSDAEF
                     EAFGVPVRSPGEKVDAFEEALRIIRGLWEEETFSFRGEHYAVEEARIEPRPGHIPIWI
                     GAYGDRMIELTGRYADGWLPSLMYVSLDRAARKMERLRAAAEAAGRDPGEITMALNVW
                     ARIEVGAEPRRGQIAGGVGRMVEEISGVLEAGFDHLNFTVSGQEQRAILAEEVLPEL"
     gene            complement(4486..5448)
                     /locus_tag="VF097_05880"
     CDS             complement(4486..5448)
                     /locus_tag="VF097_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX6262360.1"
                     /translation="MSDTLEIREAAALDAYSRVVTSVAETLTPSVASLRMKRRVRGGR
                     RAEGAGSAVVMAPDGYLLTSAHVVAAADGGSAVFVDGRSLDLEVVGTDPLSDLAVIRV
                     AADDLPAATLGDADDLRVGQLVVAVGNPMGFAGSVTAGVVSALGRSLPTRTAAAGRLV
                     ENVIQTDAALNPGNSGGALSDGESRVVGINTAVAGVGLGLAVPINDTTRGIIGALLRE
                     GRFRRSYLGITGGWRPLPPRVAERLGRQAGVEVVEVVPGSPADRAGLRPEDLILDVGG
                     ESVTDVGDLQRLMVAELIDADVRVRVFRDGEVVGVSVRPVELRT"
     gene            complement(5445..5969)
                     /locus_tag="VF097_05885"
     CDS             complement(5445..5969)
                     /locus_tag="VF097_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262361.1"
                     /translation="MTNNKEAQEEIRGWFAGRIPEGWFTEDPEITVDRDEILVVGRIE
                     PPEIEKNASEEAKREAELGRIARFREETRDKRIGVALGAERRFRTKVSWGAECGDTRR
                     LFTTLSVPVMTRLRMPERAVLDTLVDAGVARSRSHALAWCVRLVARHQGEWLEELRSA
                     FSEVERVRAEGPSL"
     gene            6065..6439
                     /locus_tag="VF097_05890"
     CDS             6065..6439
                     /locus_tag="VF097_05890"
                     /inference="COORDINATES: protein motif:HMM:NF036456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5318 family protein"
                     /protein_id="HEX6262362.1"
                     /translation="MSREPEGFGTVDYTLAKRLLLRDFGRGLLSRLDICDAHPELMRA
                     ARNVGEDMGRPCPVCDEANLVRLAYVFSDELKADNGRVWPFREALRVAARCPDGACYV
                     VEVCTECSWNHLSEAFVARSAG"
BASE COUNT          767 a         2391 c         2355 g          960 t
ORIGIN      
        1 tcgccccttc cccgtacggg cccgaggatc tcctgcggct cgggaccttg gaggagcgga
       61 ccctggaggt gcttgagacc tgcgtgcggg gtcgggccaa catcctgatc tccggcggcg
      121 ccgggtctgg gaagaccacg ctcctcggag tgctgtcctc cttcatccct gccggggagc
      181 gcatcgtgac catcgaggac gccgcggagc tgaggctccg atccgcgcac gtggtccgtc
      241 tcgaggcccg gccggcctcc gcggaggggc gcggagccgt cacggtcagg gatctcgtcc
      301 ggaacgcgct ccggatgaga cccgatcgca tcgtcgtggg ggaggttcgt ggcggagagg
      361 ccctcgacat gctccaggcg atgaacaccg ggcacgaggg ctcgatgtcc acggttcatg
      421 ccaactcgcc ccaggagctg atggcgcggc tggagacgat ggcgctgatg gccgacgttg
      481 tgctccccgc ggaccacctc cgcgagcaca tcgcccagac cgttgatctc gtcgtccaca
      541 tggtgcgcct ccgcgagggt cgcaggatcc tggcccggct gacctcggtc gaggggccgg
      601 cgggcggccg accgctgcta cgcgacgtct tccgctacgc ggggggcggc tgggaggccg
      661 tcggctcgcc gggcggcatc gcgcttcgcc agggccgcga cgagctggac ctgctcggct
      721 tctctcccaa cgggacggcc cgatgatcga ggtgggcgga cccggggtga tggggctcgc
      781 caccggggca tccatcctgg ccgcggccgc ggagcggagg cgcatgtctc gccggctcga
      841 ggctcccgtg gatccgaccg gtggccgtgc ggcggcgttg ttgggcgcgg ggctgtgcag
      901 cgtcgccgcc ggtctcctgc tgttcggccc gctcgcctcg ctgctcgcgt gcggagggct
      961 cgtggggtgg aagctcatcc accatgccag ggcgcgagaa gcagaggctc tcgagaccga
     1021 gcgggggctc agggacgcag ttcgagcgct cgcggcgtcg gctcgggtcg gcttgtcgat
     1081 ccgccgctcg ctcgaggagg tcgcacgcga gggacccccc gccgtcgcgg ggccgtttcg
     1141 gcgagcggct cgggagctgg cgatgggccg ccccctcccc gccgcgctcg cggggctctc
     1201 cgatcgactg ggttcgcggg aggccgcgat gctgaccggc gtcctgtcga tccatcatcg
     1261 tgccgggggc gacctcccga ccgcgctgga cgaggtcgcg gccctggcgg agcggcgggc
     1321 tgaggcccgc cgccgggtcc gggccctcac cgcgcagggt cgagcgtccg gggccgttct
     1381 cgctctcctt ccggtcgcct tcgtcggcct cctctccggg acgagtggga acggcctcgg
     1441 caccatctac gcgacgccgc ggggcgcggc gctgctcggc gcggggctgg cgctgcagct
     1501 cgtggggtat ctgtggatcc gagccatcct caggagggcc cagccgtgat cgtggtcggt
     1561 ctcgggtacg cggcggccgc ggccctcctg gccgcggccg ccgcgagacg cagccccgaa
     1621 ctcgcgcggc tgggcgttcc gcctcgtccc cggagggacc ctctcgaggc cctgggccgc
     1681 cgcgtgccgt ggacgcctcc cgtgctcgcg gagcgtctgg agatcctggg acgccccgcg
     1741 gaggttgacc gtctcagggg cgccgcggcc gtgtgcgccc tggtgggtct gatcggggga
     1801 agcctcacct ccgcgccatc gccggccgcg gtcggcgtcg gtgccctggc ggccgcggcc
     1861 ggctggcggc tccccgcgtt cacgttggcc cgccgcgcgg cctccgcccg agggcagatg
     1921 ctgccggagg tcgcatccct gttggatctg gtgacggtcg ggctctcggc ggggttgaca
     1981 cccaggctgg ccatcgatcg cgcggtggac cacgtggcgg agccgctccg ggaggcgctg
     2041 gtgcggatcc gccgagaggt cgccctcggc gccagctggc cctcctcgct ggaccacctg
     2101 ggccgtcgcc tcgcgctccc cgacctgggg cgcctggcct cgacgctgga tcggtccagc
     2161 cggctcgggg ccccagcggc cgacccgatc cgatccctgg cccgcgacgt ccgcgccgaa
     2221 cgctacgcgg aggtggaggc gcgagcccgg cgggctccgg tgacgatgct cttccccctc
     2281 gtgctgtgca tccttcccgc gttcgtcctg gcggcggttc tcccggcgat gctcgtcgcc
     2341 acccgaggcg tcccatgagg aggggccatg atcaggatct ggaccgctct cgtctcctgg
     2401 tggatcgttc ttcgagcccg tctcctggat cgtctctcgg ggagcaaggg acagaccacc
     2461 gccgaatacg ccctcgtgat cctggccgcg gccgcgatcg cgatcgggct gctggcctgg
     2521 gcccggtcct ccgatcgcct cccggcgttc ttcgaccgca tcatcgatcg gatcctcggc
     2581 gaggcggagt agccgggagc gcggcggttg agttcgccct cgtgcttccg ctggtcgcgc
     2641 tggtgatcgc gggtctcgtg caggtggtcc gtctgggcgg cgagtcgctc tcagtgctct
     2701 cggccgcgcg tgagggcgct cggaccgcgg cggtcaccat cgacgacggg gaggtccggc
     2761 gagctgtgat cgcctcgggc ctggacgagg cccgggccga catcctcgtg gagcggggag
     2821 gcggggtcgg cacggccgtg accgtgagcc tctcctaccg cgctccggct ggcccgccga
     2881 tcctctcgtg gctcctcccc accgaggtga ccctccgggc ccgggccacg atgcgacagg
     2941 aggtcgggtg agggagcggg ggagcgtcac gatcgtcgtc gcggtggcga tgggcatggc
     3001 cgcgatctcg gcggccctga tgggggatct gggggccgtc gcgaccggcg cggcgcgtgc
     3061 gcaggccgcc gccgacgcgg ccgccctggc ggccgcccag gagctcgtgc tcccgtccca
     3121 cgagagcccc gtggaggccg cggagcgata cgcggcgctc aacggcgccc ggttgatccg
     3181 gtgccgctgc cctccgggtg gcgaggacgc cctcgtgctg gtggagcgaa gcgtgcggct
     3241 gccgttcctc ggagggacgc ggacgctgcg ccgggccgcc cgggccaccg ttgcgctgga
     3301 gggcgacgtg gccgggctgg agccctggtt cctctcccgc ctggcgtgtc tgcgagctcg
     3361 cgtgcccgat ctctgggtcg tctccgggtt ccggaccagg gccgagcaga cgaggctgca
     3421 tgaggagaag cccgcgcttg ccgcgccacc ggggcgatcc ctccacgaac tgggcctggc
     3481 ggcggacctc ggcttcggat cggcgtcggc cggcacgctc gcccaccagc aggcaccggc
     3541 ctgcgggctc gtcttccccc tgagccacga gccgtggcac gtcgagcccg cctagagctc
     3601 ggggaggacc tcctcggcca gtatcgccct ctgctcctgg cccgacaccg tgaagttcag
     3661 atggtcgaag cccgcctcga gcaccccgga gatctcctcg accatccgac ccaccccgcc
     3721 ggcgatctgg ccgcggcgcg gttccgctcc gacctcgatt cgggcccaga cgttgagggc
     3781 catggtgatc tcgccggggt cgcgtccggc ggcctcggcc gccgcccgga gccgctccat
     3841 cttccgggcg gcccggtcca gcgagacgta catcagtgag ggcagccacc catccgcgta
     3901 gcgcccggtc agctcgatca tccggtcgcc gtacgctccg atccagatcg ggatgtgacc
     3961 cggccgcggc tcgatccgcg cctcctccac ggcgtagtgc tcgccccgga acgagaacgt
     4021 ctcctcctcc cacaggccgc ggatgatgcg gagggcctcc tcgaacgcgt ccaccttctc
     4081 gcccggtgag cgcacgggca cgccgaacgc ctcgaactcc gcgtcgctcc cgccggcccc
     4141 gatccccagc acgacccgcc cctccgagag gcggtggagc gtctcgacca tcttcgcggt
     4201 caccgcggga tgccggtagg ggagccccag cacgttcggc gagacgcgga cccggctcgt
     4261 gctggccgcg atccaggtca ggaccgtcca aggctcccag gtccgggccc cgtggaggtg
     4321 atcagagatc gtcaccatgt ccagcccgag ctcctcggca tgacgcgccg cggccacggg
     4381 gtcctctcga tgcgggagga acagcccgaa cgtcgccatg caccgactct accggcggcg
     4441 ggctcgttgg accggtggtg actccggtgc ctgacgccta acggctcagg ttcggagctc
     4501 gaccgggcgg accgagaccc cgacgacctc cccgtcgcgg aacacgcgca cccggacgtc
     4561 ggcgtcgatc agctcggcca ccatgaggcg ctggaggtcg ccgacgtcgg tcacggactc
     4621 gccccccacg tcgaggatga ggtcctcggg gcggagcccc gctctgtccg ccgggctgcc
     4681 cggcacgacc tccacgacct ccacgccggc ttggcgcccg aggcgctcgg ccacgcgggg
     4741 cgggagcggc ctccacccgc cggtgatacc gaggtaggag cggcggaacc gcccctcgcg
     4801 gagcagcgcg ccgatgatcc cgcgggtcgt gtcgttgatg ggcacggcca gtccgagtcc
     4861 caccccggcc acggcggtgt tgatgcccac gacgcgggat tcgccgtcgg agagggcgcc
     4921 cccggagttc cccgggttga gcgccgcgtc tgtctggatc acgttctcga ccagccgacc
     4981 cgccgcggcc gtcctcgttg ggagggaccg gcccagcgcg cttaccacgc cggcggtgac
     5041 gctgcccgcg aagcccatcg ggttccccac ggcgaccacg agctggccca cccgcagatc
     5101 gtcggcgtcc ccgagagtcg cggcggggag gtcgtcggcg gccacgcgga tcaccgccag
     5161 atcggagagg ggatcggtgc ccacgacctc gaggtcgagg gagcgcccgt ccacgaagac
     5221 ggcggagccc ccgtccgccg cggccacgac gtgggccgag gtcaggaggt agccgtccgg
     5281 ggccatcacg acggccgacc cggccccctc ggcccgcctc ccgccgcgga cgcggcgctt
     5341 catccggagg ctggccaccg acggggtgag ggtctcggcc accgaggtca cgacgcggga
     5401 atacgcgtcg agggcggcgg cctcgcggat ctcgagcgtg tcgctcacag cgagggcccc
     5461 tccgcgcgga ccctctcgac ctcgctgaag gccgatcgga gctcctcgag ccactcgccc
     5521 tggtgccggg cgaccagccg cacgcaccaa gcgagggcgt ggctccggct ccgggccacc
     5581 cccgcgtcca ccagcgtgtc gagcacggcc cgctcgggca tccgcagccg ggtcatgacc
     5641 ggcacgctga gggtggtgaa gaggcgtcgt gtgtcgccgc actcggcgcc ccaggagacc
     5701 ttcgtccgga agcggcgctc ggcgccgagt gctacgccga tccgcttgtc gcgcgtctcc
     5761 tcgcggaacc gggcgatgcg cccgagctcc gcctctcgct tcgcctcctc ggaggcgttc
     5821 ttctcgatct cgggcggctc gatgcgtccc accacgagga tctcgtcgcg atcgacggtg
     5881 atctcggggt cctcggtgaa ccatccttcg gggatccgtc cggcgaacca gccccggatc
     5941 tcctcctgcg cttctttgtt gttggtcatg atgattacag gattacatcg tcaccccgca
     6001 aacgtcaacg agaggcccgg gaggggaacg cgtgacgtcg ggggtgtgcc gtacaatcgc
     6061 ccccatgagc cgtgagccgg aaggcttcgg gacggtcgac tacacgctgg ccaagcggct
     6121 cctcctgcgc gacttcggtc gcgggctcct ctcccggctg gacatctgcg acgcccaccc
     6181 cgagctgatg cgcgccgcca ggaacgtggg ggaggatatg gggcggccgt gccccgtgtg
     6241 cgacgaggcc aacctcgtgc ggctcgccta cgtgttctcc gacgagctca aggccgacaa
     6301 cggccgcgtc tggcccttcc gtgaggccct gcgcgtcgcg gcgcgctgcc ccgacggcgc
     6361 ctgctacgtc gtcgaggtct gcaccgagtg ctcctggaac cacctctcgg aggccttcgt
     6421 cgcgcgcagc gccgggtagc gccggcagat ggcggccctc gggatccccc tcg
//
LOCUS       DASUTZ010000132         5945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1851748, whole genome shotgun sequence.
ACCESSION   DASUTZ010000132 DASUTZ010000000
VERSION     DASUTZ010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5945
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1851748"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VF097_05895"
     CDS             complement(<1..261)
                     /locus_tag="VF097_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010034071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX6262363.1"
                     /translation="MVVDELTDEVVDELFRALCDTTRRDILRRSALDAPSVSRLAEAY
                     PMSFAAVQKHVAVLERAGLVTKERRGREHLVHTVPEAVRRARR"
     gene            317..718
                     /locus_tag="VF097_05900"
     CDS             317..718
                     /locus_tag="VF097_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6262364.1"
                     /translation="MAGFAGEPSALTTILVVTDLERSKTWYLDVLGAELYGEYEGTSV
                     VLSFLGAWLLLVTGGGPTEDKPTVTFAEQADPDRVDHAFTIRVADCNAAYEDLRGRGA
                     EFLTPPVEHGRETRAFFRDPDGHLFEISAVT"
     gene            complement(784..1191)
                     /locus_tag="VF097_05905"
     CDS             complement(784..1191)
                     /locus_tag="VF097_05905"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX6262365.1"
                     /translation="MTDLVLDASVLLKWFAPMPERGRKEAVDLRKEFEEGRLSVIVPS
                     LIFLEVLNVAGRRWGWERHALADLASALDELGFDVREPELPSVAAWVAQGLTAYDAAY
                     VALAERSRVDLVSDDDEVFGVAGELARPLVRPA"
     gene            complement(1188..1436)
                     /locus_tag="VF097_05910"
     CDS             complement(1188..1436)
                     /locus_tag="VF097_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262366.1"
                     /translation="MAKVLVSIDDRLLARIDRAARESGLSRSGYLSRLAAREVESAPG
                     PGRDRKVRRALGRLDELFHEHPVPEDATAAVRAERNAR"
     gene            complement(1492..1563)
                     /locus_tag="VF097_05915"
     tRNA            complement(1492..1563)
                     /locus_tag="VF097_05915"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1529..1531),aa:Ala,seq:cgc)
     gene            complement(1591..2220)
                     /locus_tag="VF097_05920"
     CDS             complement(1591..2220)
                     /locus_tag="VF097_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HEX6262367.1"
                     /translation="MDLDGLAGAFQRRFDAKMGAREAALAASRRAIRSSANAIRAIHR
                     GETGVAEDLMEQAAASVEEAKAATKDHPDLLFAGYVHDAQKEYAEARVTEAIVFGKPL
                     PGPEELGVQDAPYLNGVAEAIGEARRTILDLLRQGEVDRGEELLQTMDDLYSLLVTID
                     YPDAITGNLRRSTDVARSIMEKTRGDLTIALVQKGLSDALERHREDLHG"
     gene            complement(2252..2656)
                     /locus_tag="VF097_05925"
     CDS             complement(2252..2656)
                     /locus_tag="VF097_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262368.1"
                     /translation="MFTQQRRVLMPRSDERFLGPRDRARSRLLERRRRVLTVLLEGIG
                     LTALIGFVPPLREMWVFSGILVALLGAYVWMLLQLKARRTTISLPAQPVPPVMRAPAV
                     PEPEFEVVVGPDRPIVVIPDEVAAARRARSAG"
     gene            complement(2869..4071)
                     /gene="glp"
                     /locus_tag="VF097_05930"
     CDS             complement(2869..4071)
                     /gene="glp"
                     /locus_tag="VF097_05930"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX6262369.1"
                     /translation="MRSIQEARESILRAVEPLAPIELPLQESHGCVLATDVMAEYDLP
                     PFSSAEVTGYAARAADIHAATEETPVPLRLSSGEPGRPAGGTVGWGEAVAVAAGAPVP
                     AGADCVVPLDRCRVEGGGVAVVAPVESGEFIRPAGRDVRAGETLVPAGRKLSSAEMAI
                     LAAAGRPAPPVYPRLRVGVMSIAQDLAEPGRPAAFGQAREAASFAVVGALREAGAVPY
                     RVGMVPPVEGELREAVVTNLARADSFVCTVGASDADLDPEALDVLDVEFDDVAMFPGM
                     TFGFGVVEGVSFFVLPASPVSAFVLFEMLVRPAILRMMGRRDISRPEVKAVLEEGVSG
                     PSGVTMFVPARVEHREGWRATPTGGADPDSFAALVRANGLVMIPPGDTEPAAGDEVRV
                     RIFRPLER"
     gene            complement(4074..4949)
                     /gene="galU"
                     /locus_tag="VF097_05935"
     CDS             complement(4074..4949)
                     /gene="galU"
                     /locus_tag="VF097_05935"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEX6262370.1"
                     /translation="MKVRKAVIPAAGLGTRFLPATKAQPKEMLPVVDKPAIQYVVEEA
                     VRAGIEDILIITGRGKRTIEDHFDRSFELEFYLEQAGKHEELKAVRHISDMASIHYIR
                     QKDPRGLGHAVAVAEPHVQGEPFAVLLGDDIVVANPLLERMIGVYERYGRSVIAVQEV
                     PRSDIHLYGSIKPEYVEDDLARVLDIVEKPAAEEAPSTLGAIGRYVLTPEIFEALRET
                     SPGAGGEIQLTDAIRMLASEQAVYALVYEGERFDIGKKLDYLRATIELAVDRDDLGPA
                     LRAYLADLVQRKKLL"
     gene            complement(4986..>5945)
                     /locus_tag="VF097_05940"
     CDS             complement(4986..>5945)
                     /locus_tag="VF097_05940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX6262371.1"
                     /translation="AMLSVIVDTAAATLHASTAALFLLTPSGKRLRLQAVLGYEPSAV
                     TEMRLGQGIAGRAAEGRPVLVPSRLEETPKRSPAEPPATTAIAVPLVRGPSTIGVLAL
                     YGRTLPDPFTEDDAATLASFAGQASVAIENVLLHQEAERLSLTDGLTGVWNRRYLQLQ
                     LQKEIDRAKRFGRPLSLLLVDIDRFKETNDEHGHLVGDEVLVEVVRRIVDSTRSSIDV
                     VARFGGEEFVALLPETPREGARVVAEKVRQAISLRPVQAEGVGAGIHVTVSVGVASYP
                     EDGTTVDALLRSADRAMYRAKEAGRDRVELAVSGESTARGSGG"
BASE COUNT          897 a         2176 c         2045 g          827 t
ORIGIN      
        1 gcggcgggct cggcgcaccg cctcggggac cgtgtgcacg aggtgctcgc gcccgcgccg
       61 ctccttcgtg acgaggccgg cccgctccag cacggcgacg tgcttctgca ccgcggcgaa
      121 gctcatgggg taggcctcgg ccagccgcga gacggatgga gcgtccagcg cagagcgacg
      181 gaggatgtcg cgccgcgtgg tgtcgcaaag agcgcggaag agctcgtcca ccacctcgtc
      241 ggtcagctca tctacaacca tttggttgta ggttagcgac atcgagccga tctgacaacc
      301 ctgtacgatg cgcggcgtgg ccggcttcgc gggagagccc agcgcgctga cgacgatcct
      361 cgtcgtcacg gacctcgagc ggtcgaagac ctggtacctc gacgtgctcg gggccgagct
      421 gtacggcgag tacgaaggca cgtcggtggt gctctcgttc ctcggggcct ggctgctgct
      481 cgtgaccggc ggagggccga ccgaggacaa gccgaccgtg accttcgccg agcaggccga
      541 ccccgaccgc gtggatcacg cgttcacgat ccgggtcgcc gactgcaacg cagcctacga
      601 ggacctgcgg ggccgcggcg ccgagttcct cacgccgccg gtcgagcacg ggcgggagac
      661 gagggccttc ttccgggacc cggacggcca cctgttcgag atcagcgcgg tcacgtgaga
      721 cccgcccgtg aacgagtcgg gacacgatcg ggtcgtgtcg tgccgcctcg atgcgctccc
      781 cggtcaggcc ggccgaacga gcggacgagc gagctcgccg gccacaccga agacctcatc
      841 gtcgtcggag acgagatcga cccggcttcg ctccgcaagc gcgacgtacg cggcatcgta
      901 cgccgtcagc ccttgcgcaa cccaggccgc gaccgagggg agctccggct ccctgacatc
      961 gaagccgagc tcgtccagag ccgaagcgag gtcggcgagc gcgtggcgct cccagcccca
     1021 cctcctccca gccacgttca gcacttcgag gaagatgagt gagggaacga tcaccgagag
     1081 acgaccctcc tcgaactcct tgcggagatc aaccgcttcc ttccgccccc tctcgggcat
     1141 gggcgcgaac cacttcaaca gaaccgacgc gtcgaggacg aggtccgtca ccgagcgttc
     1201 cgctcggccc tgacggccgc cgttgcatcc tccgggacgg ggtgctcgtg gaagagctcg
     1261 tccaagcggc cgagagcccg gcgaaccttc cgatcgcggc cgggaccggg ggccgactcc
     1321 acctccctgg ccgcgaggcg actgaggtag ccgctgcggc tcagacccga ctccctggct
     1381 gcgcggtcga tacgggccag gagacggtca tcgatcgaga cgagcacctt ggccatgact
     1441 cgaggatatc cggatatcca ccttttgccc agcgcagaca gcggctcgat gtggaggtga
     1501 ggggatttga acccctgacc tctgcgttgc gaacgcagcg ctctacccct gagctacacc
     1561 cccgggacgg gtcgatggta gcaccccggg tcagccgtgc aggtcctccc ggtgccgctc
     1621 cagcgcgtca ctcaggccct tctgaaccag cgcgatcgtg aggtcgccgc gggtcttctc
     1681 catgatcgag cgggccacgt cggtgctgcg ccgcaggttc cccgtgatcg cgtcgggata
     1741 gtcgatcgtc accagcagcg agtagaggtc gtccatcgtc tggagaagct cctcgccgcg
     1801 gtcgacctca ccctgcctca gcagatccag gatcgtgcgg cgggcctcgc cgatcgcctc
     1861 cgcgaccccg ttgaggtagg gcgcgtcctg gacgccgagc tcctcggggc cggggagggg
     1921 cttgccgaac acgatggcct cggtgacgcg cgcctccgcg tactccttct gggcgtcgtg
     1981 gacgtagccg gcgaagagga ggtcggggtg gtccttcgtg gcggccttcg cctcctcgac
     2041 ggaggccgcg gcctgctcca tcaggtcctc cgcgaccccg gtctcacctc gatggatggc
     2101 gcggatcgcg ttggccgagg agcggatggc cctgcgggac gcggccaggg cggcctcgcg
     2161 ggcgcccatc ttcgcgtcga agcgccgctg gaacgcgccc gcgaggccgt cgagatccac
     2221 gggcacatcc tcgcagacgc cgcgacgggc gtcagcccgc ggagcgggcc cggcgagcgg
     2281 cggccacctc gtccgggatc acgacgatcg gacggtccgg gccgaccacg acctcgaact
     2341 cgggctcggg cacggcgggc gcgcgcatga caggcggcac cggctgggcc gggagcgaga
     2401 tcgtcgtcct gcgggccttc agctgaagca gcatccagac gtaggcgccg agcagggcca
     2461 cgaggatccc gctgaagacc cacatctcgc gcagcggagg aacgaacccg atgagggcag
     2521 tcagcccgat cccctccagc agcacggtga ggacccgccg cctccgctcc agcaaccgcg
     2581 agcgagcccg gtccctcggc ccgaggaacc gctcgtccga gcgcggcatc aataccctgc
     2641 gttgctgcgt gaacaccgag aagtctcctg gtgtcgggcg caccgccgtc ttggggcgac
     2701 gcggcatgag cagtacggcc cagatgatgc ccaaaaccgc gagtagaagc caatccaacg
     2761 cccagcacct ccgtcggccg ttccccggtc gagccgccag atatgggcct tggcgcacgc
     2821 tagcacccaa gatgtagtgg ttcaaacatt tccggcgaat ctcgcgcctc agcgctccag
     2881 gggccggaag atgcgcacgc ggacctcgtc tccggccgcc ggctccgtat cgccgggggg
     2941 gatcatgacg aggccgttgg cccgcacgag ggccgcgaag ctgtccggat cggccccgcc
     3001 ggtgggcgtc gccctccagc cctcccggtg ctccacccga gcggggacga acatcgtgac
     3061 gccggacggg ccggagacac cctcctccag gaccgccttc acctcggggc gggagatgtc
     3121 gcggcgcccc atcatccgaa ggatggccgg gcggacgagc atctcgaaca gcacgaacgc
     3181 cgagacgggc gaggcgggga ggacgaagaa ggagacccct tccacgacgc cgaagccgaa
     3241 cgtcatgccg gggaacatcg ccacgtcgtc gaactcgacg tcgaggacgt ccagcgcctc
     3301 gggatccagg tccgcgtcgg acgcgccgac cgtgcagacg aaggagtcgg cccgggccag
     3361 gttcgtcacg accgcctcac ggagctcccc ctccacgggg ggcaccatcc ccacgcggta
     3421 cgggaccgcc cccgcctccc ggagggcccc caccacggcg aacgaggccg cctccctggc
     3481 ctggccgaag gcggcggggc gcccgggctc cgccaggtcc tgggcgatgc tcatcacgcc
     3541 cacccgcagc cgtgggtata cgggcggagc cgggcgcccc gccgccgcca ggatcgccat
     3601 ctccgcggac gacagcttcc tcccggccgg cacgagggtc tcccccgccc gcacgtcgcg
     3661 accggccggc cggatgaact cgcccgactc gacgggcgcc acgacggcaa cccctccccc
     3721 ctcgacacgg caccgatcga gcgggacgac gcagtcggcg ccggccggga ccggcgcgcc
     3781 ggcggccacc gccacggcct cgccccagcc gaccgttcct cccgcgggcc gacctggttc
     3841 cccggacgag agccggagcg gcaccggggt ctcctcggtg gccgcgtgga tgtcggcggc
     3901 ccgagccgcg tagccggtca cctccgcgga ggagaacggc ggcaggtcgt actcggccat
     3961 cacgtcggtg gcgagcacgc acccgtgcga ctcctggagc ggcagctcga tcggggcgag
     4021 cggctcgacc gcccggagga tcgactcgcg cgcctcctgg atggagcgca cgactagagc
     4081 agcttcttcc tctggaccag gtccgcgagg tacgcgcgga gcgccggccc cagatcgtcg
     4141 cggtcgacgg ccagctcgat cgtggcgcgc aggtagtcga gcttcttccc gatgtcgaac
     4201 cgctctccct cgtagacgag cgcgtagacg gcctgctcgc tggcgagcat cctgatcgcg
     4261 tcggtgagct ggatctctcc gcccgccccc ggggaggtct cgcggagcgc ctcgaagatc
     4321 tccggcgtca gcacgtaccg cccgatcgcc ccgagcgtgg acggcgcctc ctcggccgcg
     4381 ggcttctcca cgatgtccag gacccgcgcc aggtcgtcct cgacgtactc gggcttgatc
     4441 gagccgtaga ggtggatgtc gctgcgggga acctcctgca ccgcgatcac gctccgcccg
     4501 tagcgctcgt acaccccgat catccgctcg agcagcgggt ttgccacgac gatgtcgtcg
     4561 cccagcagca ccgcgaacgg ctcgccctgg acgtgcggct cggccacggc cacggcgtgc
     4621 cccagcccgc gcgggtcctt ctggcggatg tagtggatcg aggccatgtc ggagatgtgc
     4681 cgcaccgcct tcagctcctc gtgcttcccc gcctgctcaa ggtagaactc gagctcgaaa
     4741 gagcggtcga agtggtcctc gatcgtgcgc ttcccacgac cagtgatgat gaggatgtcc
     4801 tcgatgcccg cccggacggc ctcctcgacc acgtactgga tcgccggctt gtccacgacc
     4861 ggcagcatct ccttgggctg ggccttcgtg gccggcagga atctcgtgcc cagaccggcc
     4921 gccgggatca cggccttcct gaccttcacg ctccctccag cgtcgacggc gggtgcgccg
     4981 gaagtctagc cgcctgagcc cctcgcggtc gactcgcccg agacggccag ctcgacccgg
     5041 tcccgccccg cctccttggc ccggtacatc gcccggtcgg cggaccgcag gagcgcgtcg
     5101 accgttgtgc cgtcctccgg gtaggaggcc accccgacgc tcacggtgac gtggatgccc
     5161 gccccgactc cctccgcctg caccgggcgc agggagatgg cctggcgcac cttctcggcc
     5221 acgacgcggg cgccttctcg cggggtctcc ggcagcagcg ccacgaactc ctcgcctccg
     5281 aaccgggcca cgacgtcgat gctgctgcgg gtcgagtcca cgatccggcg gacgacctcg
     5341 accaggacct cgtcgcccac gaggtggccg tgctcgtcgt tggtctcctt gaagcggtcg
     5401 atgtcgacga ggagcaggga gagcggccgc ccgaagcgct tggcccgatc gatctccttc
     5461 tggagctgga gctggaggta tctccggttc cacacccccg tcagtccgtc ggtcagggac
     5521 aggcgctcgg cctcctggtg gaggaggacg ttctcgatcg ccaccgaggc ctggccggcg
     5581 aacgaggcga gcgtcgccgc gtcgtcctcg gtgaaggggt cggggagcgt gcggccgtag
     5641 agcgcgagca ccccgatcgt gctcgggccg cggacgagcg ggacggcgat ggcggtggtg
     5701 gccgggggct cggcaggcga ccgcttcggg gtctcctcca ggcgggacgg caccaggact
     5761 ggacggccct ccgctgcgcg gccggcgatc ccctgcccca ggcgcatctc ggtgaccgcc
     5821 gacggctcgt accccaggac tgcctggagc cgcagccgct tccccgaggg cgtgaggagg
     5881 aacagcgcgg cggtggacgc atgcagcgtc gcggcagcgg tgtccacgat cacgctcagc
     5941 atcgc
//
LOCUS       DASUTZ010000133         2796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1852093, whole genome shotgun sequence.
ACCESSION   DASUTZ010000133 DASUTZ010000000
VERSION     DASUTZ010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1852093"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VF097_05945"
     CDS             complement(<1..636)
                     /locus_tag="VF097_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEX6262372.1"
                     /translation="MTHRDRTLHVAALARVEGEGAMLVRLEGGRVAEVQLNIYEPPRF
                     FEAFLRGRRHTEPPDITARICGICPVAYQMSACRAIEAAAGVEVPEPIHRLRRLLYCG
                     EWIESHALHIYLLHAPDFLGYHGAVEMAKDHRELVERGLGLKKVGNEVLELIGGRAIH
                     PINVRVGGFYRAPTRRELGPLAEKLERAREAALETVRWVAGFDFPDFEQDYE"
     gene            complement(623..1396)
                     /locus_tag="VF097_05950"
     CDS             complement(623..1396)
                     /locus_tag="VF097_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX6262373.1"
                     /translation="MGKPTLAVWKFASCDGCQLTVLDLEDDLLPLAGEVEIAHFLEAT
                     SRSVEGPYDLSLVEGSVTTPHDAERIREVREASRFLVTIGACATSGGIQALRNFADVR
                     EFTSAVYAKPEYIATLETSTAIADHVPVDFELRGCPIDKGQLVEVVTAFLHGRRPTIP
                     NHSVCLDCKRAGHVCVLVAHGTPCLGPVTHTGCGALCPGYSRGCYGCFGPMETPDTTT
                     LSGRLRDLGMSEGDLVRVYRTFNTNAPAFKEESERHDAS"
     gene            complement(1396..2232)
                     /locus_tag="VF097_05955"
     CDS             complement(1396..2232)
                     /locus_tag="VF097_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012891381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEX6262374.1"
                     /translation="MGAPADHPGPMTPLPYRVEARRWETPDTATLALSPIDAAAPPFR
                     PGQFHMLYAFGIGEVPISVSGDADEAGPVLHTIRSVGAVTRALCALEEGDTVGLRGPF
                     GAGWDEDEAEGLDVVVVAGGLGLAPLRPAVLRLLRNRDRYNDVSVLVGAREPEGLLFA
                     EELASWHDRLHVDVTVDHASPTWRGKVGVVTELIDGAPFDPSRAVALVCGPEIMMRFA
                     VRALTERGVPPGSVRISMERNMKCAIGHCGHCQLGPEFVCKDGPVFTYERLAGWMEVA
                     EA"
     gene            complement(2216..2683)
                     /locus_tag="VF097_05960"
     CDS             complement(2216..2683)
                     /locus_tag="VF097_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6262375.1"
                     /translation="MTVEGLDRLLAEHDFFRGLEPEHLSFVAGCGSNVRFAEGDFLFR
                     EGEPADRFYIVREGRVALEVRGPGEGALVLDTLGANEVVGVSWLFPPYRWQFDARAVE
                     PVRAVALDGACLREKCDREPPLGYELMKRLSEISSQRMQSARLRMLDLYGRSG"
     gene            complement(2680..>2796)
                     /locus_tag="VF097_05965"
     CDS             complement(2680..>2796)
                     /locus_tag="VF097_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit A"
                     /protein_id="HEX6262376.1"
                     /translation="QFGTSGCVGCGRCITWCPVGIDITEEVAAIRASEEASG"
BASE COUNT          418 a         1038 c          960 g          380 t
ORIGIN      
        1 ctcgtagtcc tgctcgaagt cggggaagtc gaagccggcc acccaccgga ccgtctccag
       61 ggccgcctcc cgggcccgct ccagcttctc cgccaggggc ccgagctcgc ggcgggtggg
      121 ggcgcggtag aacccgccca cccggacgtt gatcgggtgg atcgcgcgcc ctccgatcag
      181 ctccagcacc tcgttcccga ccttcttcag cccgaggcct cgctccacca gctcgcggtg
      241 gtccttcgcc atctccaccg cgccgtggta tccgaggaag tccggggcgt ggagcaggta
      301 gatgtggagg gcgtggctct cgatccactc gccgcagtag aggagccttc ggaggcggtg
      361 gatcggctcg ggcacctcca cgccggccgc cgcctcgatc gcccggcacg cgctcatctg
      421 gtaggccacc gggcagatgc cgcagatgcg agcggtgatg tcgggcggct cggtgtgccg
      481 ccggccccgc aggaacgcct cgaagaaccg cggcggctcg tagatgttca gctggacctc
      541 ggccacgcgg ccgccctcga gccgcacgag catcgccccc tcgccctcga ccctggccag
      601 tgccgcgacg tggagcgtcc ggtcacgatg cgtcatgccg ctcgctctcc tccttgaagg
      661 ccggggcgtt cgtgttgaag gtccggtaca cgcggacgag gtcgccctcg gacatgccca
      721 ggtcccggag cctcccgctg agcgtcgttg tgtccggcgt ctccatcggg ccgaagcatc
      781 cgtagcagcc ccgcgagtag ccggggcaga gggctccgca gcccgtgtgc gtgaccggcc
      841 ccaggcacgg cgtgccgtgc gccaccagga cgcacacgtg gccggcccgc ttgcagtcga
      901 ggcagacgct gtggttcggg atcgtgggcc ggcgaccgtg gaggaaggcg gtgaccacct
      961 ccaccagctg ccccttgtcg atcgggcatc cacggagctc gaagtcgacc ggcacgtggt
     1021 cggcgatcgc ggtcgaggtc tccagcgtgg cgatgtactc gggcttcgcg tagacggcgg
     1081 aggtgaactc ccgcacgtcc gcgaagttgc gcagggcctg gatcccgccg gacgtggcgc
     1141 aggcgccgat cgtcaccagg aaccgcgacg cctcacgcac ctcgcggatc cgctcggcgt
     1201 cgtggggggt cgtcaccgac ccctcgacca gcgacaggtc gtacgggccc tccaccgagc
     1261 gggaggtggc ctcgaggaag tgggcgatct cgacctcccc ggccagcggc aggaggtcgt
     1321 cctcgaggtc cagcaccgtg agctgacacc cgtcgcagga ggcgaacttc cagacggcga
     1381 gggtgggctt gcccatcagg cctccgcgac ctccatccag ccggccaggc gctcgtacgt
     1441 gaacaccggc ccatccttgc agacgaactc ggggccgagc tgacagtgcc cgcagtgccc
     1501 gatcgcgcac ttcatgttgc gctccataga gatccggacg gacccgggcg ggaccccgcg
     1561 ctcggtcagc gcgcggacgg cgaaccgcat catgatctcc ggcccgcaca cgagggcgac
     1621 ggcccgagat ggatcgaacg gagcgccgtc gatcagctcg gtgaccaccc ccaccttgcc
     1681 ccgccacgtc ggggaggcat ggtccacggt gacgtccacg tggagccggt cgtgccacga
     1741 ggccagctcc tccgcgaaca gcaggccctc cggctcgcgc gcgccgacca gcaccgagac
     1801 gtcgttgtac cggtcccggt ttctgaggag cctgaggacg gcggggcgaa ggggggccag
     1861 tccgagcccg cctgccacga ccacgacgtc gagaccctcc gcctcgtcct cgtcccaccc
     1921 ggcgccgaag ggtccccgca ggcccacggt gtcgccctcc tccagggcgc agagcgcgcg
     1981 ggtcaccgca cccaccgaac ggatcgtgtg caggacgggg cccgcctcgt ccgcgtcccc
     2041 gctcacggag atggggacct ccccgatgcc gaaggcgtac agcatgtgga actggccggg
     2101 ccggaagggg ggcgcggcgg cgtcgatcgg ggagagcgcc agggtcgccg tgtccggcgt
     2161 ctcccatcgt ctggcctcca cccggtaggg gaggggcgtc atcgggcccg ggtggtcagc
     2221 cggagcgccc atacagatcc agcatcctga gccgggcgga ctgcatccgc tgggacgaga
     2281 tctcagacaa tcgcttcatc agctcgtacc cgaggggcgg ctcccggtcg cacttctcgc
     2341 ggaggcacgc tccgtcgagg gccaccgcgc gaacgggctc caccgctcgg gcgtcgaact
     2401 gccagcggta cggcgggaac agccaggaga cccccaccac ctcgttcgct ccgagcgtgt
     2461 cgaggacgag cgccccctcg ccgggaccgc gcacctccag cgccacacgc ccctcgcgga
     2521 cgatgtagaa gcggtcggcc ggctctccct cgcggaacag gaagtccccc tcggcgaacc
     2581 gcacgttgct cccacagccc gcgacgaagg agagatgctc gggctcgagc cctcggaaga
     2641 agtcgtgctc ggccaggagc cgatccagcc cctccacggt catccggacg cctcctcgct
     2701 cgcgcggatc gcggcgacct cctcggtgat gtcgatgccg accgggcacc aggtgatgca
     2761 gcgcccgcag cccacgcagc ccgacgtccc gaactg
//
LOCUS       DASUTZ010000134         6231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_815650, whole genome shotgun sequence.
ACCESSION   DASUTZ010000134 DASUTZ010000000
VERSION     DASUTZ010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6231
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815650"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="VF097_05970"
     CDS             complement(<1..1131)
                     /locus_tag="VF097_05970"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 domain-containing protein"
                     /protein_id="HEX6262377.1"
                     /translation="MDARNRTTDRSSRALLTAALILLAGVTGAAFGRVFEGGQPAARL
                     VLAAAAAAALAALLERRHLALSVATAAAAALVTIGLVVFPATTWFGLPTPSTLDALGR
                     ALGNLGRRAIEEAAPAPAIPALFAPSIIAVWAAVYGAHALATRAASPILALVPPAGLI
                     GFANVVAEDGARPAYAAAFLVAALAVVYTSGMRQLDLWGPVLPRRSLSAARLAAGPSG
                     RLARRLGLAATAVAVLLPGILPGFGADAVLDLDGGSGGRVAISPLVDIRPNLMRDEPL
                     ELFTIEAERPAYWRLQALDEYTGRFWRPGDPRGDLAAAVPGGRGLLPQPPGEGPALTQ
                     HVRVTRLGGRYLPAAHTPVEVAAVGLDLRHDAERGSLIVPGSL"
     gene            complement(1083..2276)
                     /locus_tag="VF097_05975"
     CDS             complement(1083..2276)
                     /locus_tag="VF097_05975"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX6262378.1"
                     /translation="MLTRRGLSVLAAGALLWVAARLTGSPDLHMVALGLLLLPALAAV
                     FIRWSRHGLRATRRLGQRRVFAGSPLRVAIEVQNTSRSAASLVLLEDRLPPSLGRAAR
                     AVLGGLRGRHRQTISYTVHCGRRGRFHIGPLIASVTDPFGLAQRKEEFPDVHELIVYP
                     HVDDLRAPSPARTAGGAGDTTARQLYQSGEEFFTMRGYEIGDDLRRIHWPSTARTGRL
                     MIRQDEAGRRASATVFLDTRRAVYGADQDLERAVSAAASVGTLYLRGGIDLRLGIIDL
                     APQRMAPEAFLEALALADPSGERQLGRSLVPLRDRSATGSALLFVTGLLADEDLGALI
                     AATGAYGQKVAVLVVAGDGEGVRAARVRLSRAEWRTVLLTPDRRLQEAWTLGTARPIA
                     AAARS"
     gene            complement(2279..3238)
                     /locus_tag="VF097_05980"
     CDS             complement(2279..3238)
                     /locus_tag="VF097_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019868908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX6262379.1"
                     /translation="MAASSDWGRFRATFDAIRANIDHVIQGKSRETRLALAALVAEGH
                     VLIEDVPGVGKTMLAKAIARSIDCSFRRIQFTPDLLPTDITGVNVFNQEQQDFEFKPG
                     AIFANIVLGDEINRASPKTQSALLESMEEHQVTVDGATYALGVPFMVIATQNPIEHEG
                     TYPLPEAQLDRFMIRLSIGYPEADAEAEILTAHGSHSALEDIDPVTDAGGVAELIAMA
                     REVHVAEPIRRYIVDLVGATRSHPDLYLGASPRASLMLMRAARAYAASEGRDYVLPDD
                     VKTLLVPVLSHRLIVAPDAAMSGHSASSLLVGLLDEIPVPVQG"
     gene            complement(3435..4238)
                     /locus_tag="VF097_05985"
     CDS             complement(3435..4238)
                     /locus_tag="VF097_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6262380.1"
                     /translation="MSAAAPSGSLLRPPDVRLRSAFRLWQRNATIYRRTYKMNLLPNF
                     FEPFLYLLAMGLGLGAYLSQVEGIRYIDFIAPGLVATAAMFGTSYDVTFNAFVKLRFG
                     KVYDAVMSTPLTPEDIALGELLWGTTRAFIYGVVFLIIASFFGVVHSWLALLAPLAIA
                     LIGMMFSVIGLAFTAVVPLIDYFTYYWTLFITPMFLFSGIFFPLDRLPDWVQSVAWFI
                     PLHQAVNLMRALMLHGDLGAAAAAAAWIVVLTAALGVLPLNLLRRRMIR"
     gene            complement(4235..5170)
                     /locus_tag="VF097_05990"
     CDS             complement(4235..5170)
                     /locus_tag="VF097_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013017067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6262381.1"
                     /translation="MRTLPGAPVVAARGLVKRYGTFEAVRGIDLDVPAGRCFGFLGPN
                     GAGKTTTMRMIATSAPPTEGHLTVLGLPVERGREIRSRMGVVPQENNLDEEITVIQNL
                     VVYARYFDIPPSVSRPRGEELLGFVALGDKRDRMPEELSGGMKRRLLIARALMSDPEV
                     LVLDEPTTGLDPQARHLVWEKMRELKRRGVTLLLTTHYMEEASQLCDRLVIMHEGRIL
                     VEGSPRELVAREVPPQVVEVLEPQDHEREHLAALEGVADHVEHLSDRVLLYGTDGETL
                     LGKVRGIVADQGSVWLRGSGLEDVFLSLTGRRLIE"
     gene            5311..5742
                     /locus_tag="VF097_05995"
     CDS             5311..5742
                     /locus_tag="VF097_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009739884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX6262382.1"
                     /translation="MTARRAVVLVLKEKAEVVESNGQVFRSEYTSFEAPSVVRLNYFV
                     KVPYRARASLTRRAVFARDDWSCQYCGKPAENVDHVVPRSRGGEHVWENVVASCRACN
                     SRKENRLLHEAGLRLRRHPYVPRDGLRLSLGRLEPEWEAYL"
     gene            5911..>6231
                     /locus_tag="VF097_06000"
     CDS             5911..>6231
                     /locus_tag="VF097_06000"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX6262383.1"
                     /translation="MRVAELVGERRYQMDPKGRVSLPVKFRESFDGGVFITLGQDGCL
                     YAFPADEWRRRAEEIDRLPISDPKARDYARIFFGKAEQVDLDSQGRLVVPRQLRETAR
                     LGKEV"
BASE COUNT          951 a         2142 c         2328 g          810 t
ORIGIN      
        1 caggctcccg gggacgatga gcgatccccg ctcggcgtcg tgccgcaggt cgagcccgac
       61 ggcggcgacc tcgacggggg tgtgcgcggc cgggaggtag cggccgccca gcctggtcac
      121 ccggacgtgc tgggtgaggg cggggccctc gccggggggc tgggggagga ggccccggcc
      181 gccggggacc gccgccgcga gatcgccccg cggatcgccc ggccgccaga agcgccccgt
      241 gtactcgtcg agggcctgga gccgccagta cgcgggccgc tccgcctcga tggtgaagag
      301 ctcgaggggc tcgtcccgca tgaggttcgg gcggatgtcc acgagcgggc tgatcgcgac
      361 ccgtcctccg gatcctccgt cgaggtcgag gacggcgtcg gcgccgaagc cggggaggat
      421 ccccgggagg agcacggcca cggccgtggc ggcgaggccc agccgccggg cgaggcggcc
      481 ggagggaccg gcggcgagcc gggcggcgga gagggatctg cgagggagga ccggccccca
      541 caggtccagc tgcctcatcc cggacgtgta caccacggcc agggcggcga ccaggaacgc
      601 cgcggcgtac gcgggacgcg cgccgtcctc ggccacgacg ttggcgaacc cgatcaggcc
      661 cgcgggcggg acgagggcga ggatcgggct ggccgcgcgg gtcgccagcg catgggcgcc
      721 gtagaccgcg gcccacaccg cgatgatcga cggagcgaag agcgcgggga tggccggggc
      781 gggggccgcc tcctcgatcg ctcgccggcc caggttgccg agcgcccggc ccagggcgtc
      841 gagggtggag ggcgtgggga gcccgaacca ggtcgtggcg gggaagacga cgagcccgat
      901 cgtgacgagg gcggccgccg cggcggtcgc caccgacagc gccaggtgtc gccgctccag
      961 gagcgccgcg agggcggccg ccgcggcggc cgccaggacg agacgcgccg ccggctgacc
     1021 gccctcgaag acgcggccga aggccgcgcc ggtgacaccg gcgaggagga tcagcgccgc
     1081 cgtcaggagc gcgcggctgc tgcgatcggt cgtgcggttc cgagcgtcca tgcttcctgg
     1141 agcctccggt ccggtgtcag gaggacggtc cgccactccg cccgcgagag acgcacccgc
     1201 gccgcgcgga ccccttcgcc gtccccggcc accacgagga cggcaacctt ctgcccgtag
     1261 gcgccggtgg cggcgatcag cgcgcccagg tcctcgtcgg cgagcaggcc ggtgacgaag
     1321 agcagcgcgg agccagtcgc gctgcggtcg cggagcggaa cgagcgagcg gcccagctgg
     1381 cgctccccgg agggatcggc gagggccaag gcctcgagaa acgcctcggg cgccatccgc
     1441 tggggggcga ggtcgatgat cccgagacga aggtcgatcc cgccccggag gtagagggtc
     1501 cccaccgagg ccgccgccga gacggcgcgc tcgaggtcct ggtcggcccc atagacggcc
     1561 cttcgcgtgt ccaggaacac ggtggctgag gcccgtcgcc ccgcctcgtc ctggcggatc
     1621 atgagacgcc ccgttcgcgc ggtggacggc cagtggatcc gccgcaggtc gtcgccgatc
     1681 tcgtacccgc gcatcgtgaa gaattcctcg cccgactggt agagctgtcg ggcggtcgtg
     1741 tcccctgcgc ccccagccgt ccgggcgggg gagggcgcgc gcaggtcgtc cacgtgcgga
     1801 tagacgatca gctcatggac gtcggggaac tcttccttcc gctgggcgag cccgaagggg
     1861 tcggtcaccg aggcgatcaa cggcccgatg tggaagcggc cccgccgccc gcagtgcacc
     1921 gtgtaggaga tcgtctggcg gtggcgaccc cggaggcccc cgagcaccgc gcgcgcggcg
     1981 cgtcccaggc tcggcggcag gcgatcctcc agcagtacca gggaagcggc cgagcggctc
     2041 gtgttctgga cctcgatcgc cacgcgcagc ggcgacccgg cgaacacgcg gcgctgcccg
     2101 agccggcggg tggcccgcag cccgtgccgg ctccaacgga tgaagacggc cgccagggcg
     2161 gggagcagca gcaggccgag cgcgaccatg tggaggtcgg gcgagcccgt gaggcgagcc
     2221 gccacccaca ggagcgcccc ggcggccagc accgagagcc cgcgccgggt cagcatgatc
     2281 agccctggac cgggacgggg atctcgtcca gcagccccac gagcagggag gacgccgagt
     2341 gcccgctcat cgcggcgtcg ggggcgacga tgaggcggtg ggagaggacc gggacgagga
     2401 gggtcttgac gtcgtcgggc agcacgtagt cgcgaccctc gctggccgcg taggccctcg
     2461 ccgcccgcat cagcatcagg gaggcgcggg ggctggcgcc caggtacagg tcggggtgcg
     2521 accgggtggc ccccacgagg tcgacgatgt agcggcggat cggctcggcc acgtggacct
     2581 cgcgtgccat cgcgatgagc tccgcgacgc cgcccgcgtc ggtcacggga tcgatgtcct
     2641 ccagcgccga gtgagacccg tgggcggtga ggatctcggc ctcggcgtcg gcctccggat
     2701 acccgatcga gagccggatc atgaagcggt cgagctgagc ctcggggagc gggtaggtcc
     2761 cctcgtgctc gatcggattc tgcgtggcga tcaccatgaa cggcaccccg agggcgtagg
     2821 tcgcgccgtc caccgtgacc tggtgctcct ccatggactc caggagggcg gactgggtct
     2881 tggggctggc ccggttgatc tcgtcgccca ggacgatgtt ggcgaagatc gctcccggct
     2941 tgaactcgaa gtcctgctgc tcctggttga agacgttcac gccggtgatg tccgtgggga
     3001 ggaggtcggg ggtgaactgg atcctccgga acgagcagtc gatcgagcgg gcgatcgcct
     3061 tggccagcat cgtcttcccc acgccgggca cgtcctcgat caggacgtgc ccctccgcca
     3121 cgagggccgc cagggccagc cgggtctccc gactcttccc ctggatcacg tgatcgatgt
     3181 tcgctcggat cgcgtcgaat gttgcccgga accgtcccca gtcgctgctc gctgccacgc
     3241 gccctccgga gtcgagtgct ccgattctac ggtgccgaag gcggaaacgg cgcagtgggg
     3301 gaggcggcgg ccgacgccct tcatccctga gacgccccgc gcggcccctg tttgggagcg
     3361 tccgtcatcc ctcgggcgcg gccgacccgc ctccgagagg attacacggg tgtcattcgc
     3421 tcgagtcaag cacctcagcg gatcatccgg cggcggagca ggttgagggg cagcaccccg
     3481 agcgccgccg tcagcacgac gatccatgcg gcggcggcgg ccgcggcccc gagatcgccg
     3541 tgcagcatca gcgcccgcat caggttgacg gcctggtgca gcgggatgaa ccacgccacg
     3601 gactggaccc agtcgggaag ccggtccagc gggaagaaga tcccgctgaa gaggaacatg
     3661 ggcgtgatga agagcgtcca gtagtaggtg aagtagtcga tcagcggcac cacggcggtg
     3721 aacgccaggc cgatcaccga gaacatcatg ccgatcagcg cgatcgccag cggagcgagg
     3781 agcgcgagcc acgaatgcac caccccgaag aacgaggcga tgatcaggaa gaccacgccg
     3841 tagatgaagg cccgcgtcgt cccccagagc agctcgccca gggcgatgtc ctcgggggtg
     3901 agcggcgtcg acatgacggc gtcatacacc ttgccgaacc gcagcttcac gaacgcgttg
     3961 aacgtgacgt cgtagctcgt gccgaacatc gcggcggtgg ccacgagccc gggcgcgatg
     4021 aagtcgatgt accggatccc ctccacctgc gagaggtagg cgccgagccc cagccccatc
     4081 gccaggaggt agaggaacgg ctcgaagaag ttcggcagga ggttcatctt gtacgtccgc
     4141 cggtagatcg tcgcgttccg ctgccacagg cggaacgccg agcgcaggcg cacgtcgggc
     4201 gggcggagca ggctcccgct cggggcggcg gcgctcactc gatcagtctc ctgcccgtga
     4261 gcgagaggaa tacgtcctcg agcccggagc cccggagcca caccgacccc tggtcggcga
     4321 cgatcccgcg caccttgccc agcagcgtct cgccgtcggt cccgtagagg aggacgcgat
     4381 cggagaggtg ctccacgtgg tcggccacgc cctcgagcgc ggcgaggtgc tcccgctcgt
     4441 ggtcctgggg ctccagaacc tccaccacct ggggcgggac ctcgcgggcc acgagctccc
     4501 gcggcgaacc ctccacgagg atccggccct cgtgcatgat caccagccgg tcgcagagct
     4561 gtgaggcctc ctccatgtag tgggtggtga ggaggagcgt gacgccccga cgcttcagct
     4621 cccgcatctt ctcccacacg aggtggcggg cctgggggtc gaggcccgtg gtgggctcgt
     4681 cgaggacgag gacctcgggg tcgctcatca gggcccgggc gatgagcagg cggcgcttca
     4741 tgccgccgga cagctcctcc ggcatacggt cgcgcttgtc gcccagcgcc acgaacccca
     4801 gcagctcctc gccgcgcggc cgcgacaccg acggggggat gtcgaagtac ctcgcgtaca
     4861 cgacgaggtt ctggatcacc gtgatctcct cgtcgaggtt gttctcctgg gggacgacgc
     4921 ccatccgcga gcggatctcg cggccccgct cgacgggcag cccgagcacg gtgaggtggc
     4981 cctcggtggg gggagcggag gtggcgatca tgcgcatcgt ggtcgtcttc cccgcgccgt
     5041 tggggcccag gaagccgaag caccgtcccg cggggacgtc gaggtcgatg ccgcggaccg
     5101 cttcgaacgt cccgtaacgc ttgacgaggc cgcgagccgc cacgacgggg gcgccgggga
     5161 gggttctcac ggcggcgagt ctagcaccgg cccccggaac cacccggcgg ctccccctat
     5221 aatggcgaca cgctccggac gatcaccggg gtcggcagag gcgtggggag agcactcgta
     5281 ctcaacgcga cgcaggagcc cctcgccgtg gtgacggcga ggcgtgccgt cgtgctcgtc
     5341 ctcaaggaga aggccgaggt cgtggagtcg aacggccagg tcttccggtc cgagtacacg
     5401 tcgttcgagg cgccctcggt cgtgcggctc aactacttcg tcaaagtgcc ctaccgggcc
     5461 agggcgtcgc tcacccggcg cgcggtcttc gcgcgtgacg actggtcttg ccagtactgc
     5521 gggaagccgg ccgagaacgt ggaccacgtg gtccccaggt cccgcggtgg ggagcacgtc
     5581 tgggagaacg tcgtcgcctc gtgccgcgcg tgcaactcgc ggaaggagaa ccggctcctc
     5641 cacgaggcgg ggctgcggct ccggcgtcat ccgtacgtcc ccagggacgg cctgaggctc
     5701 tcgctcggac gcctcgagcc cgagtgggag gcctacctct aggaccgggg ccacccgggg
     5761 cgggatccag cctcgtctga ccggcttccg ctggccacac cagtcacaga agttgacccc
     5821 tctcgactta caccactgta attccctcca gcatcttgac tgggtggagt accggtggca
     5881 taaacgtggg gcccggtgga ggagagggga gtgcgggtgg ccgaactcgt cggcgagcgc
     5941 cgataccaga tggaccccaa ggggagggtc tcgctccccg tgaagttccg ggagtccttc
     6001 gacggcggcg tcttcatcac gctggggcag gacggctgcc tctacgcgtt ccccgccgac
     6061 gagtggcgga ggcgcgccga ggagatcgat cggctcccca tctccgaccc gaaggcccgc
     6121 gactacgcgc ggatcttctt cgggaaggcc gagcaggtgg acctggactc ccagggccgc
     6181 ctggtggtcc cgcgccagct ccgggagacg gcgcggctgg gcaaggaggt c
//
LOCUS       DASUTZ010000135        10517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_297555, whole genome shotgun sequence.
ACCESSION   DASUTZ010000135 DASUTZ010000000
VERSION     DASUTZ010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10517
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297555"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..1335)
                     /gene="aroA"
                     /locus_tag="VF097_06005"
     CDS             complement(25..1335)
                     /gene="aroA"
                     /locus_tag="VF097_06005"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008436450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX6262384.1"
                     /translation="MRLVVRPSKPLRGAVAVPGDKSIAHRWLLLAATAQGGSELGGLP
                     QADDVRSTAACVLRLTASQPGLEAWLRGEPAPVRLEGRGWEGLVPPAWPLDCGNSGTT
                     MRLLAGVLAGRDFSCVLEGDESLAARPMERVAEPLRLMGAEIHTEEGHPPLQVLGGDL
                     LGIEYRLPVPSAQVQGAVLLAGVQAVGETVVDGSTFEPGSIRDHTERALAALGAPVET
                     EGGRVAVRAFQHPGLRGAVPGDPSAAAFLLAGAAILPGSDVEIRDVGVNPTRLAFASL
                     IGATVDEGRREELGEPVGTLARQGADPAPIEVPAAGLPAVVDEVPALAATAVHAGGES
                     WFRGAGELRVKESDRLRGLALGLRGLGGEAEVQGDDLVVGGDGLRGGEADSQGDHRLA
                     MALTVAALAAERESVIHDVQWAAVSFPGFVDALRALGADVEVEG"
     gene            complement(1335..2408)
                     /locus_tag="VF097_06010"
     CDS             complement(1335..2408)
                     /locus_tag="VF097_06010"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX6262385.1"
                     /translation="MRVAILGTGLIGTSIGLAARGWLDAEVAGHDPDPSALVAAAGRG
                     AVEPAGSLEGAAAAADLAFVCAPVAAIPGAAAAALEAGARVVTDAGSVKAGIVAAVEE
                     LAGGGAGRFMGGHPMTGSERSGPAGAAAGLIDGAAWALTPTDRTDPAALASVEDAVRR
                     MGAVPVTLSPDRHDRLVGLVSHLPQVASTALMSTVASSADETAPLLAAGGLRDLTRLA
                     ASSPELWVDILLANREAVAEAIEGYGDRLRSLAAMLQAGDAEGLTEAFARAKDARLGL
                     AARPQSRVGLAILTVPIPDRPGSLAEITGALAHRSVNIEDLEIVHSAEGARGTAHVTV
                     GEADAEAAVQALTSSGHEAARLA"
     gene            complement(2405..2761)
                     /gene="aroH"
                     /locus_tag="VF097_06015"
     CDS             complement(2405..2761)
                     /gene="aroH"
                     /locus_tag="VF097_06015"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX6262386.1"
                     /translation="MTRLRGIRGAVSVEADSEEAILAATGGLLREILDRNGLEREDVV
                     SIVFTATPDLRSAFPAAAARAIGLGRVPVLSAAEIDVEGALPRVVRALLHAYTERSDV
                     VHVYQGEAASLREDLA"
     gene            complement(2758..3513)
                     /locus_tag="VF097_06020"
     CDS             complement(2758..3513)
                     /locus_tag="VF097_06020"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX6262387.1"
                     /translation="MTARPRERTTPPPEPERLQKVLARAGIGSRRASEALIREGRVTV
                     NGKVATLGDRVDLSKDVVEVDGGRVPLDPGLRYYALHKPAGVVTTAKDPQGRPTVAEL
                     HPEGPRVFPVGRLDRETEGLLLLTNDGELANRLTHPRYGVEKEYLAEVEGDPSPEAIR
                     RLMRGVELEDGVAKAASASRVAGSGGRAALRLVMTEGRKREVRRMLASVGLPVARLVR
                     TRVGPIRLERLGPGETRELEPPEVAELYRASGG"
     gene            complement(3473..4042)
                     /gene="scpB"
                     /locus_tag="VF097_06025"
     CDS             complement(3473..4042)
                     /gene="scpB"
                     /locus_tag="VF097_06025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX6262388.1"
                     /translation="MDPRLRDLEAVLFVADEPLTAAALGQVLDASRAEVDGLLADLRA
                     TLEERGSGLTLRNVAGGWRLYTSAEALPAVERFVRSSRHTRFTKASLETLAIVAYKQP
                     VTRHQVSAIRGVDSDGVLRALTDRGLVVEVGREDSPGRPVLYGTTPDFLERLGLASVS
                     DLPPLAPLLGEAEEEEDDGAAPGADDPAA"
     gene            complement(4042..4800)
                     /locus_tag="VF097_06030"
     CDS             complement(4042..4800)
                     /locus_tag="VF097_06030"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEX6262389.1"
                     /translation="MTAMEEDRALARVGEFPIELPVFTGPFRVLAELLLDRKVDVCDV
                     SIAKVVEEFVGRSGEAEGWSLEEATWFLAIAAVLLELKVGRLLPKREKPFDEEDLVGG
                     SPDLVYARRLELAAFRKVAQDLALRVEASARHVAREAGPPDELVHLYPDVLEGVEPSA
                     LAETMAALLRPAPTIDLSHVTPIRLTVEDGVEAVVGHLSGRGDACFRDLVADCRDRID
                     VVVRFLALLELYRDGRVRLRQAETFGDIEVSWEG"
     gene            complement(4841..5488)
                     /locus_tag="VF097_06035"
     CDS             complement(4841..5488)
                     /locus_tag="VF097_06035"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX6262390.1"
                     /translation="MSLETILEVAYFVLALIPAVIFHEVSHGLVADRLGDPTARAAGR
                     LSLNPIRHIDPFGTVILPGLLLLPRLFGQVGAPVFGYAKPVPINPQNLREPDRQMMWI
                     AAAGPVTNLFLALVGALAFRLVGVGGGELTQFLFVFILVNVILAVFNLLPLPPLDGSK
                     VLARFLPPRAREVYRSLEAYGVLILLVIFFIFPGAIFGFITPIVRGLIELFTGLG"
     gene            complement(5485..6798)
                     /locus_tag="VF097_06040"
     CDS             complement(5485..6798)
                     /locus_tag="VF097_06040"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEX6262391.1"
                     /translation="MRRDPRDVVAAKRDGRKLPDEDMREFVLAYGRGEIDDAVAAAFV
                     MASFINGLDTDETVAMTRAMVESGETLPLDGITRPKVDKHSTGGVADSVTLIFAPLAA
                     SLGLAVAKLSGRGLGHTGGTLDKLESIPGLRTDLSPREMERQVEEVGCAVAAQTKDLV
                     PADGAFYALRDATATVPSTPLIAASIMSKKLAVGTDLVLLDMKAGSGAQMKTLTHARE
                     VASACAALAAEWGRRARVAVTDMSQPLGDAVGNALDIAEAVRALRGEEEGRLIELVVE
                     FAAAALVSLQNVPEDEGRDRARTALRDGSALESFRRMVEAQGGDSRVVDDPEGVLPGA
                     PVIEPLTSAGEGWLGKVDAEEVGKASVMLGAGRLKKGDPIDPAVGIVFHTKIGDRVER
                     GEPMGEIHARDEEAAAEAADRVRAAMTFSESEVEPPPLLYGWHGA"
     gene            complement(6795..7970)
                     /locus_tag="VF097_06045"
     CDS             complement(6795..7970)
                     /locus_tag="VF097_06045"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEX6262392.1"
                     /translation="MKVLLLVGDSFGVGAAPDAAAYGDEGSDTLGNAARAAGGIRAPN
                     LGRLGLGMLTEIEGVPPEAGPGTFHGKMAERSAGKDTTTGHWEIAGIVLDRPFPTYPD
                     GFPAEVVEPFEEAIGTRVLGNYPASGTEIIEVLGQEHLDTGRPIVYTSADSVFQIAAH
                     KEVVPLERLYEWCRVARGLLYDDHLVGRVIARPFVGEPGSFVRTHERRDFSVEPPEPT
                     YLERIEAEGIPVYGVGKIPDIYVHRGLTESEHSESNDHGVDLTLGYLSRGDHLLVVTN
                     LVDFDTSHGHRNDPVGYARCVEELDVRVPELLEAVGDDGILFLTGDHGCDPTDPSTDH
                     TREYVPVLGGGGALGDRTVDVGTRETFADLGATVADLFRVAPDGLAGTSFVPELGMR"
     gene            complement(7967..8908)
                     /gene="xerD"
                     /locus_tag="VF097_06050"
     CDS             complement(7967..8908)
                     /gene="xerD"
                     /locus_tag="VF097_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017591282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEX6262393.1"
                     /translation="MSDAHIRRFLDHLAVERGLSSNTLEAYRRDLRRYLAFLGKRGVA
                     EATRASEEDVAAFVRHLSGAEYAPERRYQASSVARALAAVRALHRFLALEGLTEDDPT
                     QGVIRPRIPRSLPKPLSVDEVADLLAAPGDTEPPALRDTAILEVLYGGGLRISELVAL
                     DVDDVDLEEGSVRVVGKGEKERVVPLGRYAVRAAEAYLARGRPALAGARTRGALFLNQ
                     RGGRLTRQGATNIIQSAARRAGIRKRVTPHVLRHSFATHLLEGGADVRVVQELLGHAS
                     VATTQIYTLVTEDRLREVYDEAHPRAKRTVSAGRRSG"
     gene            8974..9630
                     /locus_tag="VF097_06055"
     CDS             8974..9630
                     /locus_tag="VF097_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006835914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX6262394.1"
                     /translation="MAELKALFLNCTLKRSPEVSNTEALIRKVIEWFDRLDVESEIVR
                     VVDYHVPFGVTSKEGDDDEWPIILDKVKAADILVIGTPIWFGVRGSVAQMVIERLDGT
                     YQERNEVGQYPLYNKVGGVVVTGNEDGAHGAAETTLFNLSHLGCTIPPNADTYWVGDA
                     GPGPSYIEAGGEDHAYTKRTTRWMAHNLVHLARMLKANPIPAEGNTFEEEDEHAPHVG
                     "
     gene            complement(9627..10121)
                     /locus_tag="VF097_06060"
     CDS             complement(9627..10121)
                     /locus_tag="VF097_06060"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX6262395.1"
                     /translation="MTSPEIERVYEGPIFSVETLRWPGKRRDVVRALGASAVLPLTSD
                     GHVILVRQEREAVGERLLEIPAGLLDVEGEPPLEAARRELREETGHRAGSIEPLGRIH
                     TSPGFVDEVCHLFLATGCTPEGDPEEGMEVVTLPLDEAVAMVERGEITDSKTVAALLL
                     AARR"
     gene            complement(10118..>10517)
                     /locus_tag="VF097_06065"
     CDS             complement(10118..>10517)
                     /locus_tag="VF097_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX6262396.1"
                     /translation="HPVIDLLPEQQDVTDMGGTMRLGAQPCHIAEGTLAERAYGEPVI
                     YERHRHRYEVNPKYHRQLTEGGMALSGMSPDGRLVEIIELPGHPFFIAGQFHPELKSR
                     PDRPHPLFREFVGAAKAYRQAPQPAAAGAE"
BASE COUNT         1476 a         4021 c         3645 g         1375 t
ORIGIN      
        1 cgtcgatcgc caccacgggg ccgctcaccc ttccacctcc acgtcggccc cgagcgcgcg
       61 gagggcgtcc acgaagcccg ggaacgagac ggcggcccac tggacgtcgt ggatcacgct
      121 ctcgcgctcg gcggcgaggg cggccacggt cagggccatc gccaggcggt ggtcgccctg
      181 agagtcggcc tccccgccgc ggagcccatc gccgcccacc acgaggtcgt cgccctggac
      241 ctcggcctcg ccgcccaggc cccggaggcc cagggccagg ccccggaggc gatcggattc
      301 cttcacccgg agctcaccgg ccccccggaa ccacgactcc cccccggcgt ggacggcggt
      361 cgccgccagc gccgggacct cgtccacgac ggccgggagt ccggccgccg gaacctcgat
      421 cggcgccgga tcggcgccct gccgggccag cgtgccgacc ggctcgccga gctcctcgcg
      481 ccgcccctcg tcgacggtcg ctccgatgag cgatgcgaac gcgagccggg tggggttgac
      541 cccgacgtcc cggatctcga cgtccgagcc gggcaggatc gctgcgccgg ccagcaggaa
      601 ggcggccgcg gagggatcgc ccgggacggc gccccgcagg cccggatgct ggaacgcgcg
      661 gaccgcgacc cgtcctcctt ccgtctcgac cggggcgccc agcgccgcca gggcgcgctc
      721 ggtgtggtcg cggatcgagc cgggctcgaa ggtggagccg tccaccaccg tttcgcccac
      781 ggcctggacc ccggccagca ggaccgcccc ctgcacctgc gcgctcggaa cgggaagccg
      841 gtactcgatc ccgagcaggt ccccgcccag cacctggagg ggaggatggc cttcttcggt
      901 gtggatctcg gctcccatga ggcgcagcgg ctcggccact cgctccatgg ggcgggcggc
      961 gaggctctcg tcgccctcga ggacgcacga gaagtcgcgg cccgccagga ccccggccag
     1021 cagccgcatc gtcgtcccgg agttgccgca gtcgagcggc caggcgggag ggacgaggcc
     1081 ttcccacccc cggccctcga gccgcacggg ggcgggctcg cccctgagcc aggcctcgag
     1141 gccgggctgg gaggccgtga gccggaggac gcaggcggcg gtcgagcgga cgtcgtccgc
     1201 ctggggcagg ccgccgagct ccgagccgcc ctgcgccgtc gcagccagca gcagccaccg
     1261 gtgggcgatc gacttgtccc cggggacggc gacggcccct cgcagaggct tcgagggacg
     1321 gacgacgagg cgcatcaggc caggcgggcg gcctcgtggc cgctcgatgt cagcgcctgg
     1381 acggcggcct ccgcgtcggc ctcccccacg gtgacgtgcg ccgtcccccg ggcgccctcc
     1441 gcgctgtgca cgatctcgag gtcctcgatg ttgacgctcc gatgagcgag agcgccggtg
     1501 atctccgcca gcgagccggg gcggtccggg atggggacgg tcaggatcgc cagccccacc
     1561 cgggactggg ggcgagccgc gagcccgagc cgggcgtcct tcgcccgagc gaacgcctcc
     1621 gtgagcccct cggcgtcgcc cgcctggagc atggcggcca ggctccgaag gcgatccccg
     1681 tagccctcga tcgcctcggc caccgcctcg cgattggcga ggaggatgtc cacccacagc
     1741 tcggggctgc tggcggccaa acgggtgagg tcgcggagcc cgccggcggc caggaggggg
     1801 gcggtctcgt cggccgagga agccaccgtg gacatcagcg cggtcgaggc cacctgggga
     1861 aggtggctga cgaggccgac gagacgatcg tggcgatcgg ggctgagcgt cacggggacg
     1921 gcccccatcc ggcggacggc gtcctccacc gaggcgaggg ccgcgggatc cgtccggtcc
     1981 gtcggcgtga gggcccaggc ggcgccgtcg atcaggccgg cggcggcgcc ggcgggcccc
     2041 gaccgctccg agccggtcat cgggtggccg cccatgaacc ggccggcgcc gccgccggcc
     2101 agctcctcga ccgcggccac gatgccggcc ttgacgctcc cggcgtcggt gacgacgcgc
     2161 gcgccggcct ccagggcggc cgcggccgcc cccgggatcg cggccacggg agcgcagacg
     2221 aaggcgaggt cggcggccgc ggccgccccc tccagcgacc cggccggctc cacggcccct
     2281 ctccccgccg ccgctaccag tgcgcttggg tccgggtcgt gccccgcgac ctcggcgtcc
     2341 agccagcccc gcgcggcgag cccgatcgag gtcccgatca ggccggtccc gaggatcgcc
     2401 actctcatgc cagatcttcc cggagcgagg cggcttcccc ctgatacacg tgcaccacgt
     2461 cggaccgctc cgtgtaggcg tggagcagcg ccctgaccac cctgggcagc gcgccctcca
     2521 cgtcgatctc ggcggccgac agcaccggca cccttcccag cccgatggcc cgcgccgccg
     2581 ccgccgggaa ggccgatcgg agatccggcg tggccgtgaa gacgatgctc acgacgtcct
     2641 cgcgctccag cccgttccga tccaggatct cgcgcaggag cccgcccgtg gccgccagga
     2701 tcgcctcctc gctgtccgcc tccacggaca cggctccccg gatccctcgc aggcgcgtca
     2761 tccgccgctc gctcggtaca gctcggcgac ctccggcggc tcgagctccc gcgtctcccc
     2821 ggggcccagc cgctccagcc ggatcggccc cacccgggtt cggacgagcc tcgccaccgg
     2881 gaggcccacg ctcgccagca tcctccgcac ctctcgcttc cgcccctcgg tcatcacgag
     2941 gcgcagcgcc gcgcgccccc cgctccccgc cacccggctc gccgacgcgg ccttcgccac
     3001 cccgtcctcc agctcgacgc cccgcatcag ccggcggatc gcctccgggc tcgggtcgcc
     3061 ctccacctcg gccaggtact ccttctccac cccgtagcgg ggatgggtga ggcggttggc
     3121 cagctcgccg tcgttcgtca gcaggagcag tccctcggtc tcccggtcga gcctccccac
     3181 ggggaagacc cgcgggccct ccggatgcag ctccgccacc gtgggccggc cctgcgggtc
     3241 cttggccgtg gtcacgaccc cggccggctt gtggagagcg tagtagcgga ggcccggatc
     3301 gagcggcacg cgcccgccgt ccacctccac gacgtccttc gagaggtcca cgcggtcgcc
     3361 gagcgtggcc accttcccgt tcacggtgac gcgcccctct cggatcaggg cctcggaggc
     3421 gcgcctcgac ccgatccccg cacgggccag caccttctgg agccgctccg gctcaggcgg
     3481 cggggtcgtc cgctcccggg gccgcgccgt catcctcctc ctcctcggcc tcgccgagca
     3541 gcggcgccag gggcggcaga tcgctcacgg aagcgagccc cagacgctcc aggaagtccg
     3601 gcgtcgtccc gtacagcacg gggcggcccg ggctgtcctc gcgccccacc tccaccacga
     3661 gcccgcggtc ggtcagcgcc cggaggacgc catcggagtc gacgccccgg atcgcggaca
     3721 cctggtggcg ggtcaccggc tgcttgtacg ccacgatcgc cagcgtctcc agggacgcct
     3781 tggtgaacct cgtgtgcctc gaggagcgga cgaaccgctc cacggccggg agcgcctccg
     3841 cgctcgtgta caggcgccag ccaccggcca cgttccggag ggtgagtccc gacccccgct
     3901 cctccagcgt cgcacggagg tcggccagga ggccgtccac ctcggcccgc gacgcgtcga
     3961 gcacctgccc cagcgccgcc gccgtgagcg gctcgtccgc cacgaacagc accgcctcga
     4021 gatcgcgcag ccgtggatcc atcacccctc ccagctcacc tcgatgtccc cgaaggtctc
     4081 tgcctgccga agccgcaccc tcccgtcccg gtacagctcg agcagggcca ggaagcggac
     4141 cacgacgtcg atgcggtcgc ggcagtcggc cacgaggtcg cggaagcacg cgtcgccccg
     4201 gcccgagagg tgccccacca ccgcctccac cccgtcctcg acggtcaggc ggatcggggt
     4261 gacgtgcgac aggtcgatcg tcggggccgg ccgcagcagc gccgccatcg tctcggccag
     4321 cgcagacggc tccacgccct ccagcacgtc ggggtacagg tgcaccagct cgtcgggcgg
     4381 gccggcctcg cgggccacgt gccgggccga cgcctcgacc cgcagcgcga ggtcctgcgc
     4441 cacctttcgg aaggcggcga gctccagccg cctcgcgtag acgaggtcgg gcgagccgcc
     4501 gaccagatcc tcctcgtcga agggcttctc gcgcttgggg agcagccgtc ccaccttcag
     4561 ctcgaggagg acggcggcga tcgccaggaa ccaggtcgcc tcctccagcg accagccctc
     4621 ggcctcgccc gaccgcccca cgaactcctc caccaccttc gcgatcgaga cgtcgcagac
     4681 gtccaccttg cggtcgagga gaagctcggc gagcacgcgg aacgggcccg tgaagacggg
     4741 cagctcgatc gggaactcgc ccacccgggc gagcgcgcgg tcctcctcca tcgcggtcat
     4801 gggtcggggc catcgtagcc gcggccgagg ggcccctcaa tcatccgagg ccggtgaaga
     4861 gctcgatcag cccccgcacg atcggggtga tgaacccgaa gatcgccccc gggaagatga
     4921 agaagatcac gaggaggatg agcactccgt aggcctccag cgaccgatac acctcgcggg
     4981 cgcgaggcgg gaggaagcgg gccagcacct tcgagccgtc cagcggcggc aggggcagga
     5041 ggttgaacac cgccaggatc acattcacga ggatgaagac gaagaggaac tgggtcagct
     5101 cgccgccccc caccccgacg aggcggaagg ccagcgcccc gacgagcgcg aggaacaggt
     5161 tcgtgacagg gcccgcggcc gcgatccaca tcatctgacg atcgggctcc cggaggttct
     5221 gcgggttgat cggcaccggc ttggcgtagc cgaacaccgg ggcgccgacc tggccgaaca
     5281 gccgagggag cagcaggagg ccggggagga tcacggtccc gaacgggtcg atgtgcctga
     5341 tcgggttgag ggagagacgc cccgccgcgc gggcggtggg atccccgagg cggtccgcga
     5401 ccagcccgtg gctgacctcg tggaagatca ccgccgggat cagcgccagg acgaagtagg
     5461 cgacctcgag gatcgtctcg aggctcatgc gccgtgccac ccgtacagga gggggggtgg
     5521 ctcgacctcg ctctcgctga aggtcatcgc ggcgcggacg cggtcggcgg cctcggccgc
     5581 cgcttcctcg tcccgggcgt ggatctcacc catcggctcg ccgcgctcca cgcggtcgcc
     5641 gatcttggtg tggaagacga tgccgaccgc ggggtcgatc gggtcgccct tcttcaggcg
     5701 gccggcgccg agcatcaccg aggccttccc gacctcctct gcgtcgacct tccccagcca
     5761 tccctcccca gcggacgtca gcggctcgat cacaggagct ccgggtagga cgccctcggg
     5821 atcatccacg acccgagagt cgccgccctg cgcctcgacc atcctcctga acgactccag
     5881 agccgatcca tcgcgcagcg cggtccgggc ccggtcccgg ccctcgtctt ccggcacgtt
     5941 ctggagcgat acgagggcgg ccgcggcgaa ctcaaccaca agctcgatga ggcgcccctc
     6001 ctcctcgccc cgcagcgccc ggaccgcctc ggcgatgtcg agcgcattcc ccacggcgtc
     6061 ccccagcggc tgggacatgt cggtgacggc tacgcgagcg cgacgccccc actcggcggc
     6121 cagagcggcg cacgccgaag cgacctccct cgcatgcgtg agcgtcttca tctgggctcc
     6181 gctccccgcc ttcatgtcga ggaggaccag gtcggtgccc acggccagct tcttgctcat
     6241 gatgctggcc gcgatcaggg gggtcgacgg cacggtcgcg gtggcgtcac ggagggcgta
     6301 gaaggccccg tcggcgggga cgagatcctt cgtctgggcg gccaccgcgc accccacctc
     6361 ctcgacctga cgctccatct ccctcggcga gaggtcggtt cgaaggccgg ggatcgactc
     6421 cagcttgtcc agcgtgcctc ccgtgtggcc gaggccccgg cccgacagct tggcgaccgc
     6481 gaggccgagc gacgcggcga ggggcgcgaa gatcagggtc accgagtcgg ccaccccacc
     6541 cgtggagtgc ttgtccacct tcggccgggt gatgccgtcc agcgggagcg tctcccccga
     6601 ctcgaccatg gcccgggtca tcgccacggt ctcgtcggtg tcgaggccgt tgatgaacga
     6661 agccatcacg aacgcggccg cgaccgcatc atcgatctcg cctcgcccgt aggcgagaac
     6721 gaactctcgc atgtcctcat cgggcagttt ccgtccgtcc cgcttggccg ccacgacgtc
     6781 gcgagggtca cgcctcatcg catgcccagc tccgggacga agctcgtccc cgccagcccg
     6841 tccggggcca cccggaagag gtcggcgacc gtcgcgccga ggtccgcgaa cgtctcccgc
     6901 gtgccgacgt ccaccgtccg gtcgccgagg gctccaccac cccccagcac cgggacgtac
     6961 tcccgcgtgt ggtccgtgga ggggtcggtg gggtcgcagc cgtggtcccc cgtgaggaag
     7021 aggatcccgt cgtcgcccac cgcctccagc agctcgggga cgcgcacgtc gagctcttcg
     7081 acacagcgcg cgtagcccac gggatcgttc cggtgcccgt gggaagtgtc gaagtccacg
     7141 aggttcgtga cgacgaggag gtggtcgccc cgcgacaggt agccgagcgt gaggtccacg
     7201 ccgtggtcgt tggactcaga gtgctccgac tcggtgaggc ccctgtggac gtaaatgtcc
     7261 gggatcttgc ccacgccgta gacggggatc ccctcggcct cgatccgctc caggtaggtg
     7321 ggctccgggg gctcgaccga gaagtcccgg cgctcgtgcg ttctgacgaa gctccccggc
     7381 tctccgacga agggccgggc gatcacgcgc cccacgaggt gatcgtcgta caggagccct
     7441 cgcgccacgc ggcaccactc gtacagccgc tccagcggga cgacctcctt gtgcgcggcg
     7501 atctggaaga cggagtcggc gctcgtgtag acgatcgggc gcccggtgtc gaggtgctcc
     7561 tgaccgagga cctcgatgat ctccgtccct gaggctggat agttcccgag caccctcgtc
     7621 ccgatcgcct cctcgaacgg ctcgaccacc tccgccggga atccgtcggg atacgtgggg
     7681 aacgggcgat cgaggacgat gcccgcgatc tcccagtgac ccgtcgtcgt gtccttcccc
     7741 gccgagcgct cggccatctt cccgtggaac gtcccagggc ccgcctccgg cggcacgccc
     7801 tcgatctcgg tgagcatccc cagccccagg cgacccaggt tcggggcgcg gatcccgccc
     7861 gccgcccgcg ccgcgttgcc cagggtgtcg gacccctcgt ccccgtaggc cgcggcatcg
     7921 ggcgcggcgc ccacgccgaa cgagtcgccc accagcagga ggaccttcat cccgacctcc
     7981 ggccggccga aacggtccgc ttcgccctcg gatgggcctc gtcgtagacc tcgcggagcc
     8041 ggtcctcggt cacgagcgtg tagatctggg tcgtggccac gctcgcgtgc cccagcagct
     8101 cctgcaccac gcgcacgtcg gccccgccct cgagcagatg cgtggcgaac gagtgccgca
     8161 ggacgtgcgg cgtgacccgc ttgcggatcc ccgctcgccg cgcggccgac tggatgatgt
     8221 tcgtagcgcc ttggcgggtc agcctgccgc cccgctggtt caggaacaac gccccccgcg
     8281 tgcgggctcc ggccagcgcc gggcgtcctc gagccaggta cgcctcggcc gcccggaccg
     8341 cgtagcgtcc gagcggcacg acgcgctcct tttcgccctt gcccacgacc cgcacgctcc
     8401 cctcctccag gtccacatcg tccacgtcga gcgccacgag ctccgagatc cggagccccc
     8461 cgccgtacag cacctcgagg atcgccgtgt cgcggagggc cgggggctcg gtgtcgcccg
     8521 gggcggccag caggtcagcc acctcgtcca ccgagagcgg cttggggagg ctcctgggga
     8581 tccggggccg gatcacgccc tgggtcggat cgtcctcggt cagcccctcc agggccagga
     8641 accggtgcag ggcgcggacg gcggcaagcg ccctcgccac cgaggaggcc tggtagcggc
     8701 gctccggtgc gtactccgcc cccgagaggt gccgcacgaa cgccgcgacg tcctcctcgc
     8761 tggcccgggt tgcctcggcc acccctcgct tccccaggaa ggccaggtag cggcggaggt
     8821 cccgccgata cgcctccagc gtgttgctgg acagcccccg ctccacggcg aggtggtcga
     8881 ggaatcgtcg gatgtgggcg tcgctcaccg ccgaaaggat accggagccc ttcgtgccag
     8941 aatgagcccc gccccgagca gagggaggat gggatggctg agctgaaggc gctgttcctg
     9001 aactgcacgc tgaagcggtc gcccgaggtc tcgaacaccg aggcgctgat ccgcaaggtg
     9061 atcgagtggt tcgatcgtct ggacgtcgag tccgagatcg tgcgcgtggt cgactaccac
     9121 gtgcccttcg gcgtgacctc gaaggagggc gacgacgacg agtggcccat catcctggac
     9181 aaggtgaagg cggccgacat cctcgtcatc ggtacgccga tctggttcgg ggtccggggc
     9241 agcgtggccc agatggtgat cgagcggctc gacgggacct accaggagcg caacgaggtc
     9301 ggtcagtacc ccctctacaa caaggtgggc ggcgtggtgg tcaccgggaa cgaggacggg
     9361 gcgcacggtg ccgccgagac cacgctcttc aacctctccc acctggggtg cacgatcccg
     9421 cccaacgccg acacctactg ggtgggcgac gcggggcccg gcccctccta catcgaggcc
     9481 gggggcgagg accacgccta cacgaagcgc accacccgat ggatggccca caacctggtg
     9541 cacctggcgc ggatgctgaa ggcgaacccg atccccgccg agggcaacac gttcgaggaa
     9601 gaggacgagc acgcccccca cgtgggctag cggcgggcgg ccagcagcag ggcggcaacc
     9661 gtcttgctgt ctgtgatctc tccccgctcc accatcgcca ccgcctcgtc caggggcagg
     9721 gtgacgacct ccatcccctc ctcgggatcg ccctccggcg tgcacccggt cgccaggaag
     9781 aggtggcaga cctcgtccac gaaaccgggc gaggtgtgga tccgccccag cggctcgatc
     9841 gatccggcgc gatggcccgt ctcctcgcga agctcgcggc gcgcggcctc gagcggaggc
     9901 tcgccctcca cgtcgaggag cccggcgggg atctctagca gccgctctcc caccgcctcg
     9961 cgctcctggc ggacgaggat cacatggccg tccgaggtca gcgggaggac cgcgctcgcc
    10021 cccagcgcgc gaaccacgtc tcggcgcttg ccgggccagc gcagggtctc gacggagaag
    10081 atcgggccct cgtagacgcg ctcgatctcg ggcgaggtca ctcggcgccg gcggcggccg
    10141 gctgcggcgc ctggcggtac gccttggccg cccccacgaa ctcgcggaag agcgggtggg
    10201 ggcggtcggg tcgcgacttc agctcggggt ggaactggcc ggcgatgaag aacggatggc
    10261 ccgggagctc gatgatctcg acgagacggc cgtcgggcga catccccgac agcgccatgc
    10321 cgccctcggt gagctggcgg tggtacttgg ggttgacctc gtaacggtgg cggtgtcgct
    10381 cgtagatcac cggctcgccg taggcccgct ccgcgagcgt cccctcggcg atgtggcacg
    10441 gctgcgcgcc gagccgcatc gtcccgccca tatcggtgac gtcctgctgc tcgggcagga
    10501 gatcgatgac cgggtgc
//
LOCUS       DASUTZ010000136         2186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1333338, whole genome shotgun sequence.
ACCESSION   DASUTZ010000136 DASUTZ010000000
VERSION     DASUTZ010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333338"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VF097_06070"
     CDS             <1..314
                     /locus_tag="VF097_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX6262397.1"
                     /translation="RANNGIRPLKMGEKLRRKAHRHSVRMSKQRRIFHHSCLSCIAPG
                     SWSRIGENVAKAKTIRSAHRALMRSSGHRSNILCSCYSKAGMGVVKRAGWVYVTQIFL
                     G"
     gene            335..1276
                     /gene="meaB"
                     /locus_tag="VF097_06075"
     CDS             335..1276
                     /gene="meaB"
                     /locus_tag="VF097_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017620894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX6262398.1"
                     /translation="MALGDGVLSGDRRSIARAISMVEDGSEGLRELMTGLFPHTGRAL
                     TIGLTGSPGVGKSSLAAALVEGARKRDRTVAVLAIDPTSPFTGGALLGDRLRMQEHAT
                     DPGVFIRSMATRGHLGGLALAAPEAVRVLDASGVDLVAIETVGVGQAEVEVATEADTT
                     LVVVSPGWGDAVQASKAGILEIADVFVVNKADREGARETARDLEQMLHLGPELPWPPP
                     VVLTSTVNGEGMEELWEAIEGHQKHLESTGELGRRRRARLLREVEGMVAERLRVRAQS
                     LLERGDLETLADDLAARRTDPYAAADILMGKLDEEAP"
     gene            1273..>2186
                     /locus_tag="VF097_06080"
     CDS             1273..>2186
                     /locus_tag="VF097_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016907343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX6262399.1"
                     /translation="MKKEEWRQRYREAGERDADFETISGEPIEPLYTPEDLEGFDHDR
                     DLGFPGEYPYTRGVYPSMYRGRLWTMRQFAGFGSPKQTNERYKYLLKRGQGGLSVAFD
                     MPTLMGRDSDDPHAEGEVGRCGVATDSLADFEILFDGIPLDEITTSMTISGPAAILFA
                     FFLAAAEKQGVDWKAIDGTLQTDILKEYIAQKEWIFPPRPHLRIIGDLMEFCANEVPK
                     WHPISVSGYHIREAGSTAVQELAFTLADGFSYVELGKERGLDVDRFSQGLSFFFNAHI
                     DFFEEIAKFRAARRIWARWMKERYGAEDP"
BASE COUNT          350 a          749 c          775 g          312 t
ORIGIN      
        1 ctcgtgcgaa caacgggatc cggcccctca agatgggcga gaagctgcgg cggaaggccc
       61 accgccacag cgtccggatg tcgaagcagc gccggatctt ccaccacagc tgtctcagct
      121 gcatcgcccc cggcagctgg agcaggatcg gcgagaacgt ggccaaggcg aagacgatcc
      181 ggtccgcgca ccgggcgctg atgaggagct cgggccaccg gtccaacatc ctctgcagct
      241 gctactcgaa ggcgggcatg ggtgtggtga agcgcgccgg ctgggtctac gtgacccaga
      301 tcttcctggg ctgaccgact gctacgctgt ccgcatggcc ctcggggacg gcgtcctctc
      361 cggcgatcga cgctcgatag ctcgcgccat ctccatggtc gaggacggaa gcgaggggct
      421 gcgcgagctg atgacgggcc tcttccccca cacgggacgg gccctcacga tcgggctgac
      481 gggctcgccg ggcgtgggga agtcgagcct ggccgcggcg ctcgtcgagg gcgctcgcaa
      541 gcgagaccgg accgtcgccg tcctggcgat cgatcccacc tcccccttca cgggcggggc
      601 cctgctcggc gaccgcctcc gaatgcagga gcacgccacc gatcccgggg tgttcatccg
      661 ctcgatggcc acacgtgggc acctgggcgg gctcgcgctc gcggcgccgg aggccgtccg
      721 ggtgctcgac gcctcggggg tggatctcgt cgcgatcgag accgtgggcg tcgggcaggc
      781 cgaggtcgag gtcgccaccg aggcggacac gacgctcgtg gtggtctcgc ccggatgggg
      841 agacgcggtg caggcctcga aggcgggcat cctcgagatc gccgacgtct tcgtggtgaa
      901 caaggcggac cgcgagggcg ctcgcgagac cgcccgcgac ctggagcaga tgctccacct
      961 cggccccgag ctcccgtggc cacccccggt cgtcctgacc tcgacggtga acggcgaggg
     1021 catggaggaa ctgtgggagg cgatcgaggg gcaccagaag cacctggagt ccaccggcga
     1081 gctgggtcgc cggcggcgag ccaggctcct ccgggaggtc gagggcatgg tggcggagcg
     1141 cctccgggtc cgggcgcagt ccctcctgga gcggggcgac ctggagaccc tggccgacga
     1201 cctcgcggcc cgccgaaccg acccttacgc cgctgccgat atcctgatgg gtaagctcga
     1261 cgaggaggcc ccatgaagaa ggaggagtgg cgccagcgct accgcgaggc gggggagcgc
     1321 gatgcggact tcgagacgat cagcggcgag cccatcgagc ccctgtacac gcccgaggac
     1381 ctcgagggtt tcgaccacga ccgggacctc ggcttccccg gcgagtaccc atacacccgc
     1441 ggcgtctatc cctccatgta tcgaggccgg ctgtggacga tgcgccagtt cgccggcttc
     1501 gggagcccca agcagacgaa cgagcgctac aagtacctgc tgaagcgcgg gcagggcggc
     1561 ctctcggtcg ccttcgacat gcccacgctg atggggcgcg actccgacga cccccacgcg
     1621 gagggcgagg tgggccgctg cggcgtggcc acagattcgc tcgccgactt cgagatcctc
     1681 ttcgacggca tcccgctcga cgagatcacg acctcgatga cgatcagcgg gccggccgcg
     1741 atcctcttcg ccttcttcct ggccgccgcg gagaagcagg gggtggactg gaaggcgatc
     1801 gacggcaccc tccagaccga catcctcaag gagtacatcg cgcagaagga gtggatcttc
     1861 ccgccccgcc cgcacctgcg catcatcggc gacctcatgg agttctgcgc caacgaggtc
     1921 cccaagtggc accccatcag cgtctccggc taccacatcc gcgaggccgg ctcgacggcc
     1981 gtgcaggagc tggctttcac gctggccgac gggttctcct acgtcgagct ggggaaggag
     2041 cgcggcctcg acgtggatcg gttctcgcag gggctgtcgt tcttcttcaa cgcgcacatc
     2101 gacttcttcg aggagatcgc caagttccgg gcggcgcggc gcatctgggc ccggtggatg
     2161 aaggagcgct acggggcgga ggaccc
//
LOCUS       DASUTZ010000137        11100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1333967, whole genome shotgun sequence.
ACCESSION   DASUTZ010000137 DASUTZ010000000
VERSION     DASUTZ010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11100
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333967"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..959)
                     /locus_tag="VF097_06085"
     CDS             complement(150..959)
                     /locus_tag="VF097_06085"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HEX6262400.1"
                     /translation="MTHHRRDEAATLIAGGGVVALTGAGISQESGIPTFRDPGGIWER
                     FDPDVYGTWDGVMRLAMERPDDLASFLAELRAAFAGAEPGPAHAALARLERAGLLDAV
                     ITQNVDGLHQRAGSRHVVEVHGSFSRTLCLVCGNRETASSAEFLAALDRVILGLRTAF
                     IASMTSLLPACSRCGGPARPDFVAFGELPQDMAQAEELARGARVFLVVGTHGEVFPAG
                     ALPEEAAGTGATVIEVSRGHTEIRADIRLRGEAGRVLPPLVEAALTRAGRA"
     gene            complement(956..1660)
                     /locus_tag="VF097_06090"
     CDS             complement(956..1660)
                     /locus_tag="VF097_06090"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX6262401.1"
                     /translation="MDEEMTERPRATWSPLEAVPVGLGVFAGTAVATFLLDVLFDPRT
                     RFVLIGLAFEVLLGGLTLLWIRIRGASPAALGLARGGVSRRLVVGFASGLLLFTLVVF
                     VVAPAFYALLSLLAGETVIPPQQEVLPEGPSTFHVVLGGVVVVLAAPIGEELFFRGLL
                     FGGLRASMPFWAAALLSAGIFAVVHVYVLLMPLLFLVGFGLAYIYERSGSLLSSIAAH
                     AAFNLIGYLLIVRELA"
     gene            complement(1645..3348)
                     /locus_tag="VF097_06095"
     CDS             complement(1645..3348)
                     /locus_tag="VF097_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262402.1"
                     /translation="MRRAAALLALATVALPGLASGAQGPRAILVLGSPGIPYEEAISD
                     PELRAAAASGGIGLMTTHADPEAAAARTLLTGGAERDLLDVVRGEGLTAVRVEDLAGD
                     LREADLVVVGPGPVDLAGVAEAAGQAETLLVLVFPNPSPAMRERGDVVTPIVVARGPM
                     ILAEGGSVSGLTSDTTRRAGLVANVDVAPTLLAHLGVPAPGEMTGSPIELRGDPPGDL
                     HERFLESRALRLPLQLLALALALAALVAISLFLGLRPAAPELARALGGWTLIVISLPI
                     ALLPVSVLPRITWVTALALAALLTAAIAGGAWIVGDRDPTAAVALVGAAGVAALLLDS
                     VAGWPAMMAPIIGGGAFDGVRFHGIGNVYAGVLLAGAVLVAAHLRPWTGVALLGLAAL
                     FAGLPGLGSNLGAAATLAAAAGLWGVLRLRRRLGALEILIAAGAAIAGTAIVILAHRL
                     LAPDVSHIARAASDAGGLGALVEDGLRRLGSNLELTSRVPVAWLVIVALVGAAWASAR
                     RLGHLARDPEWRDACLVLALSALVGFVVNDTGIGLAGLAFAYLAAALAYPALEASWTR
                     R"
     gene            complement(3345..4616)
                     /gene="murA"
                     /locus_tag="VF097_06100"
     CDS             complement(3345..4616)
                     /gene="murA"
                     /locus_tag="VF097_06100"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018639320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX6262403.1"
                     /translation="MDRLVVTGGRRLEGAVRISGAKNSALKLMAASLLAEGMTAVHNV
                     PDILDVRTMRDVLEHLGVRVVRKDRSIAMDTSGPLGTDTPYELVRRMRASILVLGPLL
                     ARLGKARVAMPGGCNIGTRGIDLHVRGLERMGATFEAEHGYLEAQTDGLRGAVVSLDF
                     PSVGATENLLMAAVAARGTTVVENAAREPEIADLAAFLSAMGARIEGVGTPTIEIEGV
                     DAFRPAEHTVIPDRIEAGTFAIAACATEGDVLLRDARADHLDLLLAKLEETGAAIDAG
                     PEGIRVRMDRRPRAVDLVTLPYPGFPTDLQPPMLALLAGAEGTSIVTENVFERRYMFV
                     DELNRMGADIRTEGHHAVVRGVERLSAAPVRALDLRAGAAMIVAGLAADGSTEISDVH
                     HVDRGYEEIDVRLALLGAAIERRTLAPALST"
     gene            complement(4644..5048)
                     /gene="atpC"
                     /locus_tag="VF097_06105"
     CDS             complement(4644..5048)
                     /gene="atpC"
                     /locus_tag="VF097_06105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEX6262404.1"
                     /translation="MAKPLRVAVVSPEREVYAGAARMVVAKGVAGDVGILPDHAPMLV
                     ELAIHPVRVVVEEGREDVILVDGGFLNVTPSEEETRVDVLAEHAELAGEIDAAAAAAR
                     AEELKRRLEDEEDAGLRAELARAMMRAEHGRG"
     gene            complement(5049..6485)
                     /gene="atpD"
                     /locus_tag="VF097_06110"
     CDS             complement(5049..6485)
                     /gene="atpD"
                     /locus_tag="VF097_06110"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX6262405.1"
                     /translation="MAENGNGRPDGRVIQVIGPVVDVEFPPEALPEITTALELDRTIG
                     DDTDTIVCEVAQHIGASVVRAIAMKPTDGVTRGTEVRNTGSAISVPVGPKVLGHVYNV
                     TGEPLDTDSVEYEERWPIHRDPPRFEDLEPRTEMFETGIKVIDLLEPYVEGGKIGLFG
                     GAGVGKTVIIQEMIYRVAEQHGGVSIFAGVGERTREGNDLILEMTETGVIDKTALVFG
                     QMDEPPGVRLRVGLAALTMAEYFRDVQRQDVLLFIDNIFRFVQAGSEVSTLLGRMPSA
                     VGYQPTLADEMGELQERITSTKGRSITSIQAVYVPADDITDPAPHTTFAHLDATTVLS
                     RQISELGIYPAVDPLDSTSRILDPRYIGEEHYQVARTVQEILQRYRDLQDIIAILGID
                     ELSEEDKVVVSRARKIQRYLSQPFFVAEQFTGIPGKYVSREDTIRGFKMIIEGELDHL
                     PEQAFYMVGDIEEAIEQGEKLAKVGAEE"
     gene            complement(6489..7376)
                     /locus_tag="VF097_06115"
     CDS             complement(6489..7376)
                     /locus_tag="VF097_06115"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX6262406.1"
                     /translation="MGAKLRQVRRRIRSVQSTMKITRAMELIAASRIVRAQHRVLAAR
                     PYADLLTQAMEDVARNAGSLDHPLLEERSSPERAAVFVVTSDRGLAGAYNANVLRRAE
                     RLAGRLRGEGVAPTLYVAGRKGQSYFRFRGAEVEDTWTGVSEVPSYADAERIARTLIG
                     AFAQHRVDAVHAVYTDFRSAFTLRPIDKRFLPIAPEEVAGRGGEPSAEYIFEPPPEQL
                     LDGLLPRYVTAKIFHAMMESAASELASRRRAMKAATDNAEELIRQLTRVANQARQAEI
                     TTELMEVVGGAEALKQARG"
     gene            complement(7552..9096)
                     /gene="atpA"
                     /locus_tag="VF097_06120"
                     /pseudo
     CDS             complement(7552..9096)
                     /gene="atpA"
                     /locus_tag="VF097_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438085.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
     gene            complement(9101..9640)
                     /gene="atpH"
                     /locus_tag="VF097_06125"
     CDS             complement(9101..9640)
                     /gene="atpH"
                     /locus_tag="VF097_06125"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEX6262407.1"
                     /translation="MTREAEVRAYAEAILVMAEAEGVLEEVEDELFRFTRAVEQQTGL
                     REALTDPALPSDRKRAVIRDLLGGKASDATVNLLELIVEQGRARELTAIAERLVALAA
                     ERRRRAVAEVRTAVPLAEEQRSRLAEALGRATGRAVEVRVIVDPSVIGGVYARVGDQV
                     FDGTVRRRLELARERLSEV"
     gene            complement(9641..10234)
                     /locus_tag="VF097_06130"
     CDS             complement(9641..10234)
                     /locus_tag="VF097_06130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX6262408.1"
                     /translation="MNITLVGLPYTSAQEQEPVTEEPAEEEEHNPILPEVDEIIVGAL
                     AFLIVFVVLWRFAIPRLNQAMTGRTEKIQGDLEKAETERREAEELLRRYEEQLQDARS
                     ESGRIIEEARKTAEQLRRDVLERAEEESRQIVARAQEEIRAERDRAFEQLRSQVGELS
                     VELASRVVGAELDPERQRRLVDDYIDEVSGMSNGDGG"
     gene            complement(10245..10490)
                     /gene="atpE"
                     /locus_tag="VF097_06135"
     CDS             complement(10245..10490)
                     /gene="atpE"
                     /locus_tag="VF097_06135"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005392137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEX6262409.1"
                     /translation="MGDLAGLIAQVSGDVGVLGRGLVYGLAAIGPGIGIGFLVGKTVE
                     AMARQPEAAGMVRTVMFLGIAFVEALALFGLMLAFII"
     gene            complement(10504..>11100)
                     /gene="atpB"
                     /locus_tag="VF097_06140"
     CDS             complement(10504..>11100)
                     /gene="atpB"
                     /locus_tag="VF097_06140"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX6262410.1"
                     /translation="RGVQNFVESIVQFIREEIALQVIGREGLTWVPFLTALFVFILIG
                     NIFEVIPLIQFPVNSRMAIPFLLAITVWVLFIVTGIRLHGLSYFKGVLFPPGVPWPIY
                     ILLTPIELISTFIVRPITLSVRLLANMVAGHMILTVFFLGTVYLMLSLPLGPLVGVPA
                     FALSVALVAFEMLVSVLQAYIFTILTAVYIGGAIHPEH"
BASE COUNT         1656 a         4081 c         3852 g         1511 t
ORIGIN      
        1 cgagaggaag gcgctcgcga ggagcgccac gagaagcgcc cgcggtgcgc tgagcctccg
       61 agtcataggg tttgtatcgg cctgggcgta ggccgacctg aacccgcacc ctacacgact
      121 cggtctcccg aatcacgcac cctggctgct caggccctgc cggcccgcgt cagcgcggcc
      181 tcgacgaggg gagggagcac ccgcccggcc tcgccgcgga gacggatgtc cgcccggatc
      241 tccgtgtggc cgcgggagac ctcgatcacc gtggcccccg tccccgccgc ctcctcgggg
      301 agggccccgg ccggaaacac ctcaccgtgc gtccccacga ccaggaagac ccgggccccg
      361 cgggccagct cctcggcctg agccatgtcc tgggggagct cgccgaacgc gacgaagtcc
      421 ggccgggctg gcccgccgca gcgggagcac gccggaagga gcgaggtcat gctggcgatg
      481 aaggcggtcc ggagccccag gatgacccgg tcgagcgccg cgaggaactc ggccgaggag
      541 gcggtctctc gattcccgca cacgaggcac agcgtccggg agaaggaccc gtgcacctcg
      601 accacgtgcc ggctccccgc ccgctggtgg agaccgtcca cgttctgagt gatcacggcg
      661 tcgagcaggc cggcgcgctc caaccgcgcc agggccgcgt gcgcgggccc cggctcggca
      721 cccgcgaagg cggccctgag ctcggcgagg aaggaggcga gatcgtcggg ccgttccatg
      781 gccaggcgca tcacgccgtc ccaggtcccg tacacgtcgg gatcgaagcg ctcccagatc
      841 ccgccgggat cgcggaacgt ggggatgccc gactcctgcg agatgcccgc gccggtgagc
      901 gccacgaccc ccccgcccgc gatgagcgtc gccgcctcgt cccgacgatg atgggtcacg
      961 caagctcccg gacgatcagc aggtacccga tcaggttgaa cgccgcgtgg gccgcgatgc
     1021 tggacagcag cgagccgctg cgctcgtaga tgtaggcgag cccgaagccc acgaggaaga
     1081 gcaggggcat gaggagcacg tagacgtgga cgaccgcgaa gatccccgcc gagagaagcg
     1141 ccgccgccca gaagggcatc gaggcgcgga ggccaccgaa caggagcccc cggaagaaga
     1201 gctcctcccc gatcggggcc gccagcacga cgaccacgcc gcccagcacg acgtggaagg
     1261 tggaggggcc ctccgggagc acctcctgct gcggagggat caccgtctcg ccggccagga
     1321 gcgacagaag ggcgtagaag gccggggcga ccacgaagac gaccagcgtg aagaggagga
     1381 ggcccgaggc gaaccccacc accagccggc gggagacccc gcccctagcg agcccaaggg
     1441 cggccgggga cgccccccgg atccggatcc agaggagggt gaggcccccc agcaggacct
     1501 cgaacgccag gccgatcagc acgaacctcg tccgagggtc gaacagcacg tccaggagga
     1561 aggtcgccac ggcggtcccc gcgaagacac cgagcccgac ggggacggcc tcgagcgggc
     1621 tccaggtggc ccgggggcgc tcggtcatct cctcgtccac gatgcctcca gcgcagggta
     1681 ggccagggcc gccgcgaggt aggcgaacgc cagcccggcc aggccgatcc cggtgtcgtt
     1741 caccacgaag cccaccaggg ccgagagagc gaggacgagg caggcgtccc gccactcggg
     1801 gtcgcgggcc aggtgcccga gacgcctggc cgaggcccac gccgcgccga cgagcgcgac
     1861 gatcacgagc caggcgacgg ggacgcgcga ggtgagctcc aggttgctcc ccaggcgccg
     1921 taggccgtcc tcgaccaggg cgccgaggcc tcctgcgtcg gaggcggccc gagcgatgtg
     1981 agagacgtcg ggggccagga gccggtgagc caggatcacg atcgccgttc cggcgatcgc
     2041 agccccggcg gcgatcagga tctcgagcgc gcccaggcgt cggcggagcc ggaggacccc
     2101 ccagagcccg gccgcggccg cgagcgtggc ggcggccccc aggttcgacc ccagcccggg
     2161 caggcccgcg aagagcgccg ccaggcccag cagcgccaca cccgtccagg gccggaggtg
     2221 ggccgcgacg agcaccgccc ctgccaggag gacccccgcg tagacgttgc cgatcccgtg
     2281 gaagcggacc ccgtcgaacg ccccgccgcc gatgatcggg gccatcatgg ccggccagcc
     2341 ggcgaccgag tccaggagga gagcggcgac accggccgcg ccgacgagcg ccaccgccgc
     2401 cgtggggtcg cggtcgccca cgatccacgc accgcccgcg atcgcggcag tcagcagggc
     2461 cgccagcgcc agcgccgtca cccaggtgat cctggggagg acgctgaccg ggaggagcgc
     2521 gatcgggagc gagatcacga tcagcgtcca tccgccgagg gcccgcgcca gctccggtgc
     2581 ggcggggcgg agcccgagga agagcgagat ggcgacgagc gcggcgaggg cgagggccag
     2641 cgcgaggagc tgcaggggaa gccgcagggc ccggctctcc aggaaccgct cgtggagatc
     2701 ccccggcggg tcgccccgga gctcgatcgg agacccggtc atctccccgg gggccgggac
     2761 cccgaggtgc gcgagcaggg tgggggccac atccacgttc gccacgaggc cggcccggcg
     2821 cgtggtgtcc gaggtgagcc ccgagacgga gccgccctcg gcgaggatca tggggccccg
     2881 agccacgacg atgggcgtaa ccacgtcccc ccgctcgcgc atcgccgggg agggattggg
     2941 gaagacgagg accaggagcg tctccgcctg gccggcggcc tccgcgaccc ccgcgagatc
     3001 gaccggcccc gggcccacga ccacgaggtc cgcctcgcgg aggtcgccgg ccaggtcctc
     3061 gaccctcacc gcggtcagcc cctctccgcg cacgacgtcc aacaggtcgc gctcggcgcc
     3121 tccggtcagg agcgtgcggg cggcggcggc ctccgggtcc gcgtgggtcg tcatcagccc
     3181 gatccccccg gaggccgccg ccgcccgcag ctccgggtcg gagatcgcct cctcgtacgg
     3241 gatcccgggg ctccccagga ccaggatcgc ccgggggccc tgggccccgg aggccagccc
     3301 ggggagcgcg acggtcgcga gcgcgaggag ggccgccgcc ctcctcacgt ggacagcgcg
     3361 ggagcgagcg tccgccgctc gatcgcggcg cccagcaacg ccaggcgtac gtcgatctcc
     3421 tcgtagcccc gatcgacgtg atggacgtcc gagatctcgg tcgagccgtc ggcggcgagg
     3481 cccgccacga tcatcgccgc cccagcccga aggtcgaggg cccgtaccgg ggcggccgag
     3541 aggcgctcca ccccgcgcac gacggcgtgg tggccctcgg tccggatgtc cgcgcccatc
     3601 cggttcagct cgtccacgaa catgtaccgg cgctcgaaca cgttctcggt gacgatgctc
     3661 gtcccctccg ccccagccag gagggccagc atcggcggct ggagatcggt ggggaacccc
     3721 gggtacggga gggtcacgag gtcgaccgcc cgggggcgcc ggtccatccg cacgcggatc
     3781 ccctcgggcc cggcgtcgat cgccgccccc gtctcctcga gcttggccag caggagatcg
     3841 agatggtccg cgcgggcgtc ccgcagcagg acgtccccct cggtggcgca cgccgcgatg
     3901 gcgaacgtcc ccgcctcgat ccgatccggg atgaccgtgt gctccgccgg ccggaaggcg
     3961 tccacaccct cgatctcgat cgtgggcgtc cccacgccct cgatgcgcgc ccccatcgcc
     4021 gagaggaacg ccgcgaggtc ggcgatctcg ggctcgcgcg ccgcgttctc cacgacggtc
     4081 gttccgcgcg cggccaccgc cgccatgagg aggttctcgg tcgcgccgac gctggggaag
     4141 tcgagggaga cgacggcgcc gcggaggccg tcggtctgcg cctccaggta cccgtgctcc
     4201 gcctcgaagg tggcgcccat ccgctccagc ccgcggacgt ggaggtcgat gccccgcgtc
     4261 ccgatgttgc agccgcccgg catcgccacg cgcgccttcc ccagcctcgc cagcagtggc
     4321 cccagcacga ggatcgaggc ccgcatccgg cggacgagct cgtagggggt gtcggtcccg
     4381 agggggccgg aggtgtccat cgcgatcgag cggtccttcc gcacgacccg cacgccgaga
     4441 tgctcgagca cgtcgcgcat cgtgcggacg tcgaggatgt cgggcacgtt gtggacggcc
     4501 gtcatcccct cggccaggag ggaggcggcc atcagcttga gggcggagtt cttcgccccg
     4561 ctgatccgga cggcgccctc gaggcgccgg ccgccggtca cgacgagccg gtccatggtc
     4621 ccgtgattct acgcgcccga ggctcagccc cggccgtgct cggcccgcat catggccctg
     4681 gccagctcgg cgcggaggcc ggcgtcctcc tcgtcctcga ggcgccgctt cagctcctcg
     4741 gcgcgcgcgg cggccgcggc ggcgtcgatc tcgcctgcca gctccgcgtg ctcggcgagc
     4801 acgtcgacgc gggtctcttc ctcgctcggg gtcacgttga ggaagcctcc gtccacgagg
     4861 atcacgtcct cgcgcccttc ctcgacgacg acgcggaccg ggtggatcgc cagctcgacc
     4921 agcatcgggg cgtggtccgg caggatcccc acgtccccgg ccacgccctt ggccacgacc
     4981 atccgggccg cgcccgcgta gacctcgcgc tcgggagaga ccacggcgac tcggagcggc
     5041 ttcgccacct actcctccgc gcccaccttg gccagcttct cgccctgctc gatcgcctcc
     5101 tcgatgtccc ccaccatgta gaaggcctgc tcgggcaggt gatccagttc gccctcgatg
     5161 atcatcttga acccgcggat cgtgtcctcc ctcgagacat acttcccggg gatccccgtg
     5221 aactgctcgg ccacgaagaa cggctgggag aggtaccgct ggatcttccg agccctcgac
     5281 acgacgacct tgtcctcttc ggagagctcg tcgatcccca ggatcgcgat gatgtcctgg
     5341 agatccctgt accgctggag gatctcctga accgtccgcg ccacctgata gtgctcctcg
     5401 ccgatgtagc gggggtcgag gatccgcgac gtcgagtcga gggggtccac cgccgggtag
     5461 atcccgagct ccgagatctg cctcgagagt accgtcgtcg cgtcgaggtg ggcgaacgtg
     5521 gtgtgcgggg ccgggtcggt gatgtcgtcg gccggcacgt agacagcctg gatcgacgtg
     5581 atcgaccgcc ccttcgtcga ggtgatccgc tcctgcagct cacccatctc gtcggccagc
     5641 gtgggctggt acccgacggc gctcggcatc cgcccgagga gcgtggagac ctcggacccc
     5701 gcctgcacga accggaagat gttgtcgatg aacagcagca cgtcctgtcg ctggacgtcg
     5761 cggaaatact cggccatggt cagggcggcc agcccgacgc gcaggcgcac gccggggggc
     5821 tcgtccatct ggccgaacac gagcgcggtc ttgtcgatga cccccgtctc ggtcatctcc
     5881 aggatgaggt cgttgccctc gcgggtccgc tcccccaccc ccgcgaagat cgagacgccg
     5941 ccgtgctgct cggcgacccg gtagatcatc tcctggatga tgaccgtctt gcccacgccc
     6001 gcgcccccga acaagccgat cttcccgccc tccacgtatg gctccagcag gtcgatgacc
     6061 ttgatgccgg tctcgaacat ctcggtccgc ggctcgaggt cttcgaacct gggcgggtcg
     6121 cgatggatcg gccaccgctc ctcgtactcc accgagtcgg tgtcgagagg ctccccggtc
     6181 acgttgtaga cgtgccccag caccttcggc cccaccggga cgctgatcgc agagccggtg
     6241 tttcggacct cggtgccgcg ggtgacgccg tcggtgggct tcatcgcgat cgcacgcacc
     6301 accgacgccc cgatgtgctg ggcgacctcg cagacgatcg tgtcggtgtc gtccccgatc
     6361 gtccggtcga gctcgagcgc cgtggtgatc tccggcagcg cctcgggcgg gaactccacg
     6421 tccacaaccg ggccgatcac ctggatcacg cgcccatcgg gcctgccgtt gccgttctca
     6481 gccatcgctc atcctctcgc ttgcttcagg gcctccgccc ctccgaccac ttccatcagc
     6541 tcggtggtga tctccgcttg gcgggcctgg ttcgcgaccc gggtgagctg gcggatcagc
     6601 tcctcggcgt tgtcggtggc ggccttcatc gcccgacgcc gcgaggcgag ctccgaggcg
     6661 gccgactcca tcatcgcgtg gaagatcttc gccgtgacgt agcgcggcag cagcccgtcg
     6721 agcagctgct ccggaggggg ctcgaagatg tactccgccg aaggctctcc tccccggccg
     6781 gcgacctctt ccggcgcgat ggggaggaag cgtttgtcga tcggtcgaag ggtgaacgcc
     6841 gagcgaaagt ccgtatagac ggcgtggacg gcatcgacgc gatgctgggc gaaggcgccg
     6901 atcagggtcc gcgcgatgcg ctcggcatcg gcgtaggagg gcacctcgct cacgccggtc
     6961 caggtgtcct cgacctcggc gccccggaac cggaagtagg actgcccctt ccgcccggcg
     7021 acgtagaggg tcggggccac gccttcgccc cgcagtcgtc cggcgagccg ctcggctcgg
     7081 cggagcacgt tggcgttgta ggcccccgcg agcccccggt cggaggtcac cacgaaaacc
     7141 gcggcccgct cggggctcga ccgctcctcg aggagggggt ggtccaagga gccggcgttg
     7201 cgcgccacgt cctccatcgc ctgggtcagg aggtcggcgt acggccgcgc ggcgagcacg
     7261 cggtgctgcg cccggacgat gcgtgatgcg gcgatcagct ccatggcgcg cgtgatcttc
     7321 atcgtggact ggaccgagcg gatccgacgc cggacctggc gaagcttcgc gcccatgcta
     7381 tcccgggggc ggggcgtcgg cgtcgctgat cggacccgcc tcctcctcgg ggccggaggg
     7441 ctccgccccg gcgtcgtcgg gagcctcctc gcccacgcgg tcccagccga cgtcctcctt
     7501 cagctcgtcg accgggccgc cgccgatcac gccggcgccg ccggccggcg cctcgctcgg
     7561 ggagaactgg gaccggaagg cctccgccgc cgtgcggagc tcctcttcga tctcgtccgt
     7621 cagggtgccc tccgctcgca ggtggtcgta gatcccgccg tggcgcgtcg ccacgtactc
     7681 gaggagctcg tgctcgaagc gcttgcagtc gtcgatcggg atctcgtcga ggaagccgtt
     7741 cgtcacggcc cagatcgaga cgacctgccg atccaccggc atcggggcgt actggggctg
     7801 cttcaggact tcgacgaccc gcgccccacg ctcgagctgc tgctgggacg ccttgtcgag
     7861 ctcggatccg aactcggcga acgcctcgag ctcgcggaac tgcgccaggt cgatccggag
     7921 acggccggcc acctgcttca tcgccttgat ctgcgcgtcg ccgcccaccc gggagaccga
     7981 aatccccacg ttgatcgccg gccgcacgcc ggagttgaac agatcggttt cgaggtagat
     8041 ctggccgtcg gtgatcgaga tcacgttcgt ggggatgtag gcggagacgt cgccgccctt
     8101 cgtctccacg atcgggagcc cggtgagcga tccgcccccg tggtcgtcgt cgagcttcgc
     8161 gcagcgctcc agcaaccgcg agtggaggta gaagacgtcg cccggatacg cctcgcggcc
     8221 ggcggggcgc cgcaggagca gcgagacctg acggtacgcc tcggcctgct tcgagaggtc
     8281 gtcgaagacg acgagcgcgt gctggccctt gtacatccaa tgctggccga tcgccgaccc
     8341 ggcgtacggg gcgatgtact tgaaggcggc cggcacggac ccgggagcgg tgaccacgac
     8401 cgtgtagtcc atcgccccgc gctcctcgag ggccgccacc acctctgaca cggtggaggc
     8461 cttctgtccg atggcgacgt acacgcactt cacctggagc gccgggtcgc cgctggccca
     8521 gttctctcgc tgctggatga tcgtgtcgat cgcgatcgcc gtcttccccg tctgacggtc
     8581 gccaatgatc agctcgcgtt gtccgcggcc gatcggggtc atgccgtcga tcgccttgat
     8641 cccggtctgg agcggctcgt tcaccggctg gcgcatcacg accgaggggg cctggagctc
     8701 cagcgcgcga tcggcctccg cctgaacggg ccccttcccg tccagcggca cgcccagtgg
     8761 gtccaccaca cgtcccagga ggccgtctcc caccggcacg gagaggatgc ggccggtccg
     8821 cttgacgggg tctccctcct cgatcgcctc ggcctcgccg aacacgacca cgccgatctc
     8881 gtcctcctcg aggttgaacg ccaccccgaa gacgtctccc cggaactcga ggagctcgtt
     8941 ggcgagggca ccggggaggc cccgcacgcg ggcgatccca tccccggtct cgacgacgcg
     9001 gcccacctcc tcctcgcgca cggacggctc gaagctctcg acgtgcctgc gcagcgcctc
     9061 cgcgatctcg tcgggcctga tggtcagctc cgccatgtcc tcacacctcg ctcagccgct
     9121 cgcgcgccag ctcgaggcgg cgccggacgg ttccgtcgaa cacctggtcg cccacgcggg
     9181 cgtagacgcc gccgatcacc gaggggtcca cgatgacgcg gacctccacc gctcggccgg
     9241 tggcccgtcc cagcgcctcg gccagccgac tgcgctgctc ctcggccagg ggcaccgcgg
     9301 tccggacctc ggccacggcg cgccggcggc gctcggcggc cagcgccacg agccgctcgg
     9361 cgatcgccgt cagctcgcgg gcccgaccct gctccacgat cagctcgagg aggttcaccg
     9421 tggcgtcgct ggccttacct ccgaggaggt cccggatcac ggcccgcttg cggtcggacg
     9481 gcagggccgg gtcggtcagc gcctcgcgca gcccggtctg ctgctccacg gcgcgcgtga
     9541 accggaagag ctcgtcctcg acctcctcca ggacgccctc ggcctcggcc atcacgagga
     9601 tggcttccgc gtaggcccgg acctcagcct cgcgcgtcac ctagcctccg tccccgttcg
     9661 acatgccgga cacctcgtcg atgtagtcgt ccacgagacg gcgctgacgc tcggggtcga
     9721 gctcggcgcc cacgacgcgg gaggccagct cgaccgagag ctccccgacc tgggaccgga
     9781 gctgctcgaa ggctcggtcg cgctcggctc ggatctcctc ctgagctctg gccacgatct
     9841 ggcgggactc ttcctcggcc cgctccagca cgtcccgccg cagctgctcg gccgtcttcc
     9901 gggcctcctc gatgatgcgg ccggactcgc tccgggcgtc ctggagctgc tcctcgtacc
     9961 gcctgaggag ctcctcggcc tcgcgtcgct cggtctcggc cttctccagg tcgccctgga
    10021 tcttctcggt acggccggtc atggcctggt tcaggcgcgg gatggcgaac ctccacagga
    10081 cgacgaagac gatgaggaag gccagcgcgc cgacgatgat ctcgtcgacc tcggggagga
    10141 tcgggttgtg ctcctcctcc tccgccggct cctcggtgac cggctcctgc tcctgagccg
    10201 aggtgtaggg cagcccgacc agcgtgatgt tcatcctggc cacctcagat gatgaaggcc
    10261 agcatgaggc cgaacagggc cagcgcctcg acgaaggcga tgccgaggaa catgaccgtc
    10321 cgcaccatgc ccgcggcctc gggctgcctc gccatcgcct cgacggtctt cccgacgagg
    10381 aacccgatgc cgatgccggg gccgatggcg gcgaggccgt agacgaggcc ccggcccagg
    10441 acgcccacgt cgccggacac ctgtgcgatc aggccagcga gatctcccat agctgctctc
    10501 ctctcagtgc tccgggtgga tagcgccgcc gatgtacacg gcggtcagga tcgtgaagat
    10561 gtatgcctgc aggacagaca ccagcatctc gaacgccacg agtgccacgg acagcgcgaa
    10621 ggccgggacc cccacgagcg ggcccagtgg gaggctcagc atcaggtaga cggtccccag
    10681 gaagaacacg gtgaggatca tgtgccccgc caccatgttg gcgaggagac ggaccgacag
    10741 cgtgatgggc cggacgatga acgtcgagat cagctcgatc ggggtcagca ggatgtagat
    10801 gggccagggg acccccgggg ggaagagcac gcccttgaag tacgagagcc cgtgcagccg
    10861 gatgccggtg acgatgaaca ggacccagac cgtgatcgcc aggaggaaag ggatcgccat
    10921 ccgggagttc acggggaact ggatcagggg gatgacctcg aagatgttgc cgatgaggat
    10981 gaagacgaag agcgccgtca ggaacgggac ccacgtcagg ccctcgcgac cgatcacctg
    11041 cagcgcgatc tcctcgcgga tgaactgcac gatcgactcc acgaagttct gcacgcctcg
//
LOCUS       DASUTZ010000138         3081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_946917, whole genome shotgun sequence.
ACCESSION   DASUTZ010000138 DASUTZ010000000
VERSION     DASUTZ010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946917"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..1438
                     /locus_tag="VF097_06145"
     CDS             92..1438
                     /locus_tag="VF097_06145"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6262411.1"
                     /translation="MGRRALLVAGLVVVVMSAAELAAASTSRRASGEDPLPIEIEVVA
                     LPNPFSPNGDGRRDEAVIAVTSSAPGSVTVSIQDAAGETWRAWPELPLPEPGSVEVVW
                     RGRAGDVRAPDGRYEVVAAIATEDDASATAAAQLVLDRQPPRLRRLRVRPDPLTTHRR
                     LVIRFVGRDRAPRLRAELRIEDSLGAIGKQGAERPSGSTRVRWRARYRSGKRLYPGNY
                     RLGVRLWDDAGNVSPRRWVPWRVHRNVAGRVYRRLEGTGRRVALTFDDCGNPGAWASI
                     LSTLRAKHVEAAFFCGGGAVRGNPALARRTVRDGHTPGSHALDHAILTGHDSSFTSAR
                     VRADRRAWWQVARRTSAPYLRPPYGAWNAAVVEGAAAAAAPRVVLWDVNPQDWRSPGA
                     AEIRLRVLSAARPGSIILLHTGDQTAAAVGPIVDGLRARNLEPVGLSALFRAAGMR"
     gene            complement(1440..1907)
                     /locus_tag="VF097_06150"
     CDS             complement(1440..1907)
                     /locus_tag="VF097_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262412.1"
                     /translation="MDTMAGTTLVRGDVLPGTPLIDAATGAEVRIWDFRGRTALVLCF
                     LHPACEACERFAAGLAEMESEFRLTGARAFAVLREPSPSALPVLVDGHGEASRRLLGP
                     EGELPTIVVTDRYAAAWESYPVLGHDFPPVSEIVGDLWHLATICEECGIVAWQ"
     gene            1939..2178
                     /locus_tag="VF097_06155"
     CDS             1939..2178
                     /locus_tag="VF097_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262413.1"
                     /translation="MEASCVVCGRATYDPDKRERPWARGVAGGRLVLICPRCQGERPD
                     WPRSLDACAACGSTRLSITLGEVICRECGHTSGAS"
     gene            2378..>3081
                     /locus_tag="VF097_06160"
     CDS             2378..>3081
                     /locus_tag="VF097_06160"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX6262414.1"
                     /translation="MRDIGLTGLPGVGKSTLFTALTHTGAAGGRSNQAVVPVPDPRLE
                     ILAEIEGSARIVPAQVRFVDVPGGLSSQAIATLREADALAIVLAAFHPAADPAADLGT
                     IRAELLLADLANAERGLERAERRAKGRAEEALADLDRLSRARDLLEAERPLREGGWSD
                     DDLKAMRGYGFLTLKPWIVVANLQEGAGLPEALPEGTVAVYAAIEAEVSAMDPEEGAA
                     LLREFGVEEPGLRKLIR"
BASE COUNT          417 a         1123 c         1115 g          426 t
ORIGIN      
        1 tgacacctgt cacgaggcgg ccccctcctg cccacgctcc gcgggtccta cggccctctc
       61 cgcacccgcc ctctctttct atcgtgtagg cgtgggtcgt cgtgcgctgc tcgtcgctgg
      121 actcgtcgtg gtcgtcatgt cggcggccga gctggccgcg gcctccacga gcaggcgcgc
      181 gagcggagag gatcccctgc cgatcgagat cgaggtcgtc gccctcccga accccttctc
      241 ccccaacggg gacggccgcc gagacgaggc tgtgatcgcc gtgacgagct cggcgccggg
      301 ctcggtcacg gtgtcgatcc aggacgctgc cggcgagacg tggcgcgcgt ggccggagct
      361 cccccttccc gaaccgggat ccgtcgaggt cgtgtggcgg ggacgggcgg gggacgtgcg
      421 cgcccctgac ggccgctacg aggtcgtggc cgccatcgcc acggaggacg acgcgagcgc
      481 caccgccgcc gcgcagctcg tcctcgacag acagccgccc cgcctacgcc gcctccgcgt
      541 tcggcccgat cccctcacaa cccaccggcg gctcgtgatc cggttcgtgg ggcgggatcg
      601 cgcccccagg ctccgcgcgg agctccgcat cgaggattcc ctcggggcga tcgggaagca
      661 gggggcggag aggcccagcg gctcgacgcg cgtgcgctgg agggcccgct atcggagcgg
      721 gaagaggctc tacccgggca actaccggct gggcgtccgc ctgtgggacg acgccggcaa
      781 cgtatccccg cgtcggtggg tgccctggcg ggttcaccgc aacgtggcgg gccgcgtgta
      841 ccgacgcctc gaggggaccg ggcggcgcgt ggccctgacg ttcgacgact gcgggaaccc
      901 cggagcttgg gccagcatcc tctcgaccct ccgtgccaag cacgtcgagg ccgcgttctt
      961 ctgcggagga ggcgccgtcc gggggaaccc tgctctggcc cgacgcacgg ttcgcgacgg
     1021 gcacacgccc gggtcccacg cgctggacca cgcgatcctg acggggcacg actcctcctt
     1081 cacctccgcg cgggtgcggg ccgaccgccg ggcctggtgg caggtcgccc ggcgcacctc
     1141 ggccccctac ctccgcccac cctacggcgc ctggaacgcg gcggtcgtgg aaggagcggc
     1201 cgcggccgcg gcgcctcgcg tcgtgctgtg ggacgtcaat ccccaggact ggcggagccc
     1261 cggcgcagcc gagatcaggc ttcgggtgct gtcggcggcg cgaccgggca gcatcatcct
     1321 gctgcacacg ggagatcaga cggccgccgc cgtgggcccc atcgtggacg gcctgcgggc
     1381 gaggaacctc gagccggtcg gcctctcggc gctcttcagg gctgcgggga tgcgctgagt
     1441 cactgccaag cgacgatgcc gcactcctcg catatcgtgg cgaggtgcca gaggtccccc
     1501 acgatctccg atactggcgg gaagtcgtgc cccaggacgg ggtaggactc ccacgccgcc
     1561 gcgtaccggt cggtcacgac gatcgtcggg agctcgccct ccggcccgag gagccgccgg
     1621 ctcgcctcgc cgtgtccatc cacgaggacc gggagcgccg agggcgaggg ttcacggagc
     1681 acggcgaacg cgcgtgcgcc cgtcagccgg aactcgctct ccatctccgc gagcccggcc
     1741 gcgaaacgct cgcacgcctc gcaggccgga tggaggaagc agaggaccag cgccgtcctg
     1801 ccgcggaagt cccagatgcg gacctctgcg ccggtggcgg cgtcgatcag gggcgtgccg
     1861 ggcagcacgt ccccacggac cagcgtcgtt cccgccatcg tgtccacgcc atccatgcta
     1921 gccccctatc gtgtgggcat ggaggcctcg tgtgtggtgt gcggccgggc tacctacgac
     1981 cccgacaagc gggagcgacc gtgggcgagg ggtgtggcgg gcgggcggct cgtgctcatc
     2041 tgcccgcggt gccagggtga gcggcccgac tggccccgga gcctcgacgc gtgtgccgcc
     2101 tgcggaagca cgcgcctgtc catcaccctc ggtgaggtga tctgccgcga atgcgggcac
     2161 acgtccgggg cgtcgtgact caccagccgc agcagggctc gatgtatcga caggccggac
     2221 accggtagtg ggcgtgctcg ggctccatcg cctcaccgca cagggggcac gggaggcgct
     2281 tcgactccga ctcgcaggcc aatgtctctt ctcgcacgag ccgaacctac ccggcgtcac
     2341 cgcccgggtc acgcaccctt ggtagcgtag gcgaccgatg agggacatcg gtctgaccgg
     2401 acttcccggc gtcgggaagt ccacactgtt caccgcgctg acccatacgg gggccgcagg
     2461 cgggcgctcg aaccaggccg tcgtcccggt gccggaccct cggctggaga tcctggcaga
     2521 gatcgagggc tcggcgcgga tcgtcccggc tcaggtgcga ttcgtcgacg tccccggcgg
     2581 gctctcgtcc caagcgatcg ccacgctgcg cgaggccgac gctctcgcca tcgtgcttgc
     2641 ggccttccac cccgcggcgg accccgcggc cgacctcggg acgatccgcg ccgagctgct
     2701 gctggccgat ctggcgaacg ccgaacgcgg cctggagcgg gcggaacgca gggccaaggg
     2761 gagggccgag gaggcgctcg ccgacctcga ccgtctctcg cgggcccgcg acctcctcga
     2821 ggcagagcgt ccgcttcgcg aggggggatg gtcggacgac gacctgaagg cgatgcgtgg
     2881 atacgggttc ctcacgctga agccctggat cgtggtggcc aacctgcagg aaggggccgg
     2941 cctgcccgag gctctccctg agggaaccgt cgcggtctat gcagcgatcg aggcggaggt
     3001 ctccgccatg gaccccgaag aaggcgcagc cctcctccgt gagttcgggg tcgaggagcc
     3061 cggcctgcgg aagctgatcc g
//
LOCUS       DASUTZ010000139         3288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1723372, whole genome shotgun sequence.
ACCESSION   DASUTZ010000139 DASUTZ010000000
VERSION     DASUTZ010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1723372"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..659
                     /locus_tag="VF097_06165"
     CDS             219..659
                     /locus_tag="VF097_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6262415.1"
                     /translation="MARTTAMEREAADLLGRTSLFSELSKRELLAIARAGKEVRRPEG
                     ATLAQEGEAGVGFFLILEGDAAVSIAGRPAGVLIKGDTFGEVALLDGGPRTASVEATS
                     PVRLFGLTQWAFTRVVREYPTVALKLLQVLAGYVRNSASGLPQL"
     gene            698..1843
                     /locus_tag="VF097_06170"
     CDS             698..1843
                     /locus_tag="VF097_06170"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262416.1"
                     /translation="MDLLRPRDLRTLVEAPSGPSVSIYMPSHRAGPEVRQDPIRLKNL
                     LGRAEERLLSDGLRRPEAGEILDPAGDLLEDEDFWRHQGDGLALFLSKDHGLVLRLPV
                     AFEELLVTGDRYHIKPLIPLLSGDARFLILAVSQKEIRLLQATRQVVEEVELEDVPES
                     LREAAVHREMRFLNYHVGTGFSSGGRQSAIFHGHGDRTDEEYVRKYMRAIDEGLRSAI
                     EGERSPLVLAGVRFLLDTYRTVSQYPDILPEIVQGNPDELSNQELHAQARPLVEARHR
                     RRVEEAVGRFEEQAGTGLAVSGVHEVVPAAAHGRVEVLWVGLGVQRWGSFDEETASVE
                     VRDQPKPGDEDLLDRAAADALLSGGTVYAVDPGDVPGDAPIAALLRY"
     gene            complement(1837..2250)
                     /locus_tag="VF097_06175"
     CDS             complement(1837..2250)
                     /locus_tag="VF097_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262417.1"
                     /translation="MEVTTAMLADAATVENGKLYVHGGAWSVINVRELPVTHPSMALA
                     LVFRVEYTEALEDHPIVIELLDEDDQPVGPRIDGIIHVGHPPRMPPGTPSFVPQAIRF
                     NNIRLEREGGYRFRVTVRDDEVASVQFRVLRTRGQ"
     gene            2328..3284
                     /locus_tag="VF097_06180"
     CDS             2328..3284
                     /locus_tag="VF097_06180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008424305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX6262418.1"
                     /translation="MLQIGYTLSSEEFGPQDLVAFARRAEETGFSFALVSDHYHPWID
                     RQGHSPFVWSVLGGVSQTTERLRIGTGVTCPTIRIHPAIIAQAAATAACMLPGRFFLG
                     VGSGENLNEHVLGDRWPAAAVRLDMLEEAIDVIRLLWEGETTSHRGEHYVLENARVYT
                     LPDERPPIYVAASGPRSLRIAGGLGDGLIGVAPDPEMVETFRDAGGEGKPAYGQLHVC
                     WAESEEDAVRTALEWWPNTSLPGQLGQELPLPRHFEQAARLVTEDVIREQVVCGPDPE
                     RFLDAIREYERAGFDHVYLHQIGPDQEGFFRFSEAEILPKIG"
BASE COUNT          463 a         1173 c         1183 g          469 t
ORIGIN      
        1 ggtgaaggtg gcccgcgggc tgtgggagca ctacgactcg ccctacacgc tgatgggcgt
       61 gatgggcgtg aacatccagc tccacgccct caccggcggg ctcctgatcc tcggctacct
      121 gatcgcgatc tgggcggata ggctcatgga ctcggtcccc ctcttcctcc ggtagcccgg
      181 ggcggcgtag aatcccgaac gaccgcggga ggcgcgtgat ggcgaggacg acggcgatgg
      241 agcgggaggc ggccgacctt ctcgggcgga ccagcctgtt ctcggagctc tccaagcgcg
      301 agctgctggc gatcgcccga gccggcaagg aggtccgccg gccggagggc gccaccctgg
      361 cccaggaggg tgaggccggg gtcggcttct tcctcatctt ggagggcgac gccgcggtct
      421 cgatcgccgg caggcccgcc ggcgtcctca tcaagggcga caccttcggc gaggtggccc
      481 tcctcgacgg cggcccccgg accgcgagcg tcgaggccac gagcccggtg cggctcttcg
      541 gcctcaccca gtgggccttc acccgggtgg tccgcgaata tccgaccgtg gcgctgaagc
      601 tgctgcaggt gctcgccggc tacgtccgca actcggcgtc ggggctccct cagctctgag
      661 cgcgtggccg tcaccgaccc ggggtaccct gcccgacatg gacctgctcc gcccgcgaga
      721 cctccgaacc ctcgtcgagg cgccctcggg gccctccgtg tccatctaca tgcccagcca
      781 ccgggccggc ccggaggttc ggcaggaccc gatccgcctg aagaacctcc tgggccgggc
      841 cgaggagcga ctgctgtccg acgggctgcg ccgtccggag gccggggaga tcctcgaccc
      901 cgccggtgac ctcctcgagg acgaggactt ctggagacac cagggcgacg gcctggcgct
      961 cttcctgtcg aaggaccacg gcctcgtcct ccgcctgccc gtggcgttcg aggagctcct
     1021 ggtgaccgga gaccgctacc acatcaagcc cctgatcccc ctgctctccg gcgacgcccg
     1081 cttcctgatc ctcgcggtga gccagaagga gatccggctc ctccaggcca cccgccaggt
     1141 ggtcgaggag gtcgagctgg aggacgtccc cgagtccctg cgcgaggcgg ccgtccaccg
     1201 cgagatgcgc ttcctgaact accacgtggg cacggggttc tcgagcggcg gccggcagtc
     1261 ggcgatcttc cacggccacg gcgaccggac cgacgaggag tacgtccgga aatacatgcg
     1321 ggcgatcgac gaggggctcc ggtcggcgat cgagggcgag cgctcgccgc tcgtgctggc
     1381 cggcgtccgc ttcctgctgg acacgtaccg aaccgtcagc cagtaccccg acatcctccc
     1441 ggagatcgtc caggggaacc ccgacgagct ctccaaccag gagctgcacg cgcaggctcg
     1501 gccgctcgtg gaggcccggc accgccggcg ggtcgaggag gcggtcgggc ggttcgagga
     1561 gcaggccggg accgggctcg cggtgagcgg cgtgcacgag gtggtgcccg cggccgccca
     1621 cgggcgcgtc gaggtgctgt gggtcgggct cggggtccag cggtggggct ccttcgacga
     1681 ggagactgcg tcggtcgagg tgcgggacca gccgaagccc ggcgacgaag acctgctcga
     1741 ccgggccgca gccgacgcgc tgctctcggg ggggacggtc tacgcggtgg atccgggcga
     1801 cgtcccgggc gacgcgccga tcgccgccct cctccgctac tgaccccggg tcctcaggac
     1861 ccggaactgc accgacgcca cctcgtcgtc ccggaccgtg acgcggaacc ggtacccgcc
     1921 ctcgcgctcg aggcggatgt tgttgaagcg gatcgcctga gggacgaacg agggggtgcc
     1981 cggcggcatc ctgggcgggt ggccgacgtg gatgatcccg tcgatccgcg gaccgaccgg
     2041 ctgatcgtcc tcgtccagaa gctcgatcac gatggggtgg tcctcaagcg cctccgtgta
     2101 ctcgacgcgg aatacgaggg ccagggccat gctggggtgg gtgaccggca gctcccggac
     2161 gttgatcacg ctccaggcgc cgccgtggac gtagagcttg ccgttctcca cggtggcggc
     2221 gtcggcgagc atcgcggtgg tgacctccac gcccccatga ttcccgcagg agggccgatc
     2281 cgccaccccg tcgcatggaa cctcccggcc gcgggtaccc acacggcatg ctccagatcg
     2341 gctacacgct ctccagcgag gagttcggtc cccaggacct ggtggcgttc gcccggaggg
     2401 ccgaggagac ggggttctcg ttcgcgctcg tctcggatca ctaccatccg tggatcgacc
     2461 gccagggcca cagcccgttc gtgtggtcgg tgctgggggg cgtctcccag acgaccgagc
     2521 gcctccggat cggcaccggg gtgacctgcc ccacgatccg gatccacccc gcgatcatcg
     2581 cccaggccgc tgccaccgcc gcctgcatgc tgcccggtcg cttcttcctg ggcgtgggca
     2641 gcggcgagaa cctcaacgag cacgtgctgg gcgatcggtg gccggcggct gccgtgcggc
     2701 tcgacatgct cgaggaggcg atcgacgtga tccgcctgct gtgggagggg gagacgacga
     2761 gccaccgggg agagcactac gtcctggaga acgcccgcgt gtacacgctg cccgacgagc
     2821 ggcccccgat ctacgtagcg gcctcgggcc cccgctcgct ccggatcgcc gggggcctcg
     2881 gcgacggcct gatcggcgtg gccccggacc cggagatggt cgagacgttc cgcgacgctg
     2941 ggggcgaggg gaagccggcc tacggccagc tccacgtgtg ctgggccgag agcgaggagg
     3001 acgcggtccg gacggcgctc gagtggtggc ccaacacctc gctcccgggc cagctgggcc
     3061 aggaactgcc actcccccgc cacttcgagc aggccgcccg gctcgtgacc gaggacgtga
     3121 tccgggagca ggtcgtctgc gggcccgacc ccgagcggtt cctggacgcg atccgcgagt
     3181 acgagcgggc ggggttcgac cacgtgtacc tccaccagat cgggccggat caggagggct
     3241 tcttccggtt ctccgaggcg gagatcctcc ccaagatcgg ctgacccc
//
LOCUS       DASUTZ010000140         5717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_167734, whole genome shotgun sequence.
ACCESSION   DASUTZ010000140 DASUTZ010000000
VERSION     DASUTZ010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167734"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..691)
                     /locus_tag="VF097_06185"
     CDS             complement(110..691)
                     /locus_tag="VF097_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262419.1"
                     /translation="MALALAGLIAAPSSAAVRDPNGADVPFYARIEPGGFAASDGDLT
                     MVYFYRSPECVPAGFNLLDFFDIPGAFSCRPLTTEGFTIWETGVFLDPAPFFSKLSGL
                     GAVPVWFVDASDYDAAISDGELTIGELEGLNPLKGAASQFNEVLRPSEVEKTIPMLAV
                     RLAGHLDDGRPFRANHVHVHVNDVPSTTNITIG"
     gene            complement(1031..3955)
                     /locus_tag="VF097_06190"
     CDS             complement(1031..3955)
                     /locus_tag="VF097_06190"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6262420.1"
                     /translation="MGDAPHSPNLLERDVQLQAIGAAIGAAGQGAGQAVLFEGAAGIG
                     KTSLLAAAASRGALEGLRVLSARGDELERHFPWGVAIQLFAGAVVGPDRQKLMSGAAG
                     LTAPLFEGGAPEVRGPPDLFPLVHGLHWLTANLAERSPLLLLLDDIQWSDPESLRFLH
                     YLVLRLEELPASLVITVRTGDPVDPEKEDLLARLRAHPAILGHGVDPLDPDSIRSLVR
                     SELPEADDAFCDAVARAVAGNPFFCHGLIAAARAEEIEPTQSGAARLRELRPEAVHDT
                     ILVRLGRLGGAAGRLAAATAVLGVRATLPRAASLAGLDDASAAQAVDALAAADILEGG
                     SSLSFVHPIIAEIVHADLTPSRLRQNHLTAARLLLEDGAPAEEVAAHLLRGGPAGEEW
                     AAAVLGEAGGIMLARGAPTRAAELLARALEEPGGRDDPGLLLQLGRTETALGSPTAVQ
                     RLEAAAAHARNPVERATALGALGFARYIAGETAAAARTTREGLGEIEPGRGGALEAEL
                     LLNLLAGRFVPELVEETTRAFEQPRTGERGDPTPAEIVRRAMLAFDTILRLGAAPAKA
                     DAEWAFRHGFETRALSPLPLASAMIGVSLLFVGHYRKAAQIIEHELESARSRGNRLDA
                     AIAYEARAGLRWMVGDVGGILADTESGLEVSEGRWDQASVLTRVLRVTALLERRDDAE
                     AAAVLDVPEALERRLPGTLAWVWLPYGRAHIAFAASDWAGAAQQALTAGKRLTEVSAS
                     SPDYMAWRSLAARALHRGGDRDRALRVAEEELGFARANGSARAIGNASATVGLLRGGE
                     AGIRMLWEAIDVLDRHGARLAAANARVDLGILLRHGRRAKQARRPLAEALDAARRLGS
                     VRLADTARSELSAAGGRPRRLALTGVESLTPGQRRVTDLAAEGMSNREIAEALFVTVR
                     TVETHLTAAYGKLGISSREELPAALRGQQNDPGSPGASAPGPANQRPFGPRSEPPTRS
                     SR"
     gene            4261..4980
                     /locus_tag="VF097_06195"
     CDS             4261..4980
                     /locus_tag="VF097_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX6262421.1"
                     /translation="MSSFFYNLGKRAGPAIRKGRWVWSSLSGSEAEARRAERAVGREL
                     AGRTRSRFGVDDDLRDSRLLEEIGFSLVAKDRNADREFSFEPLRGGGMNAFALPGGFV
                     FVTRGLLDDCGRDRDELAFVLAHEIGHVVRGHALERLVGRSALAGVVRALPAAGLLGR
                     LAGRPSLALLESAHSREQEYEADSVGVRLSMSAGFDPSGSSRFLERLVRPERAVVANY
                     LASHPDPEQRIRRIEEGRSPG"
BASE COUNT          897 a         1986 c         1941 g          893 t
ORIGIN      
        1 tcctctactt tcttgtccgc ttcgcttcgc gtagccacag ccgcgggacc tgcgcctgct
       61 cgggagcgcc gttgaagacc ggagagctca cttctgctat ccgccctcgt cagccgatgg
      121 tgatattggt tgtcgaggga acatcgttca catggacgtg gacatggttg gctcggaacg
      181 gccggccgtc atcgagatga cccgccaatc gaacagccag catcggaatc gtcttctcca
      241 cctcggaggg gcgaaggacc tcgttgaact ggcttgcggc gcccttgaga gggttcaagc
      301 cttcaagttc tccgatggtg agttcgccgt ccgagatcgc ggcgtcgtaa tcactggcgt
      361 ccacgaacca cactggtacc gcgccgagtc cggatagctt gctgaagaac ggggcggggt
      421 cgaggaacac tccggtctcc cagatcgtga acccctctgt cgtgaggggc cgacaggaaa
      481 acgcgccagg gatgtcgaag aaatcgagaa gattgaagcc agccggcacg cattcgggcg
      541 agcggtagaa gtacaccatc gtcaggtccc catccgaagc ggcgaacccg cctggctcga
      601 tacgcgcgta gaacgggaca tcggcaccgt tcgggtcacg aacggcggcg ctcgacggcg
      661 ccgcgatcaa cccggccaag gccagggcca tagccatcac aattagacgt cgcatcggtt
      721 ctcctcctct cgtgtgcacc cttccactcc aatgtccgaa cttgactcac gcattagagg
      781 gctatgccac agccccctgt catcgtggga cgaccacgag gttttcgagc cgccgccgcg
      841 ctaatctcgt ggtctgccca cggtgaccac gccgggagtc gttcgtagca tcggccggtg
      901 aacagcgacc gggagggcgg gcgagtccct gagggcgagc cacccgatga agtaagcgtg
      961 cgcctgacgg tcgaccgcct ccctggaccg atctccggca ccctcacggt cgaggacgag
     1021 acggtcgaat tcagcggctg gatcgagttg gtggctctga tcgaggcccg aagggccgtt
     1081 ggttcgcggg ccccggagcc gaggcgccgg gggaccccgg atcgttctgc tgtccccgaa
     1141 gggccgccgg aagctcctct cgggagctga tcccgagctt gccgtaggcc gcagtgaggt
     1201 gggtctcgac ggttcgcacc gtgacgaaca gcgcctcagc gatctctcga ttcgacatcc
     1261 cctcggctgc caggtcggtg acacgccgct gcccgggggt gagcgactcg acgccggtca
     1321 acgcgagccg ccgagggcgg ccgcccgcgg cgctcaactc actgcgcgcc gtgtcggcga
     1381 gcctcacgga accgagcctg cgcgccgcat ccagggcctc tgcgagcggg cggcgcgcct
     1441 gcttcgcccg ccgcccgtgt cggaggagga tgcccagatc gacgcgggcg ttcgccgcgg
     1501 ccagcctggc tccgtggcgg tcgagcacgt cgatcgcttc ccacagcatc cggatcccgg
     1561 cctccccacc ccgtaagagc ccgacggtcg cgctcgcgtt ccctattgcc cgtgcggagc
     1621 cgtttgcgcg cgcgaacccg agctcctcct cggcgacgcg gagagcacga tcgcgatcgc
     1681 cgccgcgatg aagggcgcga gcagcgagcg aacgccaggc catgtagtcg gggctcgagg
     1741 cgctgacctc ggtcagccgt ttcccggccg tgagcgcctg ctgggcggcg ccggcccagt
     1801 cgctggcggc gaaggcgatg tgtgccctcc cgtacggaag ccagacccag gccagggtgc
     1861 cgggaagccg ccgctcgagt gcttccggca catcgagcac cgctgccgcc tccgcgtcgt
     1921 cccgcctctc caggagggcc gtcacccgca ggacccgggt caggaccgag gcctgatccc
     1981 aacgtccctc gctcacctcc aggcccgact ccgtatcggc caggatgccg ccgacgtctc
     2041 ctaccatcca ccgcagaccc gcccgcgcct cgtatgcgat cgcggcgtcg aggcggttac
     2101 ctcgggaacg ggcgctctcc agctcgtgct cgatgatctg ggcggccttt cggtagtgac
     2161 caacgaagag caggctcacg ccgatcatcg cgctggcgag cgggagcggc gagagagccc
     2221 gtgtctcgaa tccgtgccgg aaggcccact cagcatcggc cttggcaggt gctgcgccga
     2281 ggcgcaggat cgtgtcaaag gcgagcatcg ctcgcctcac gatctccgcc ggcgtcggat
     2341 ctcctcgctc ccctgtccgc ggctgttcga aagccctggt cgtctcctcc accagctccg
     2401 gcacgaaacg acccgccagg agattcaaga ggagctccgc ctcgagcgcc ccaccgcggc
     2461 cgggttcgat ctccccgagg ccctctcggg tcgtccttgc cgcggcggcg gtctctccgg
     2521 cgatatatcg ggcgaatccc agcgctccga gcgcggtcgc gcgctcgacc ggattgcggg
     2581 cgtgcgccgc cgctgcctcg agtcgctgaa cggcggtcgg gctcccgagt gccgtctcgg
     2641 ttcgcccgag ctggagcagc agcccgggat cgtctcttcc tcccggctcc tccagcgcac
     2701 gggccagcag ctcagcggcc cgggtgggcg caccgcgcgc cagcatgatg ccgccggcct
     2761 ccccgaggac ggcggcggcc cactcctcgc ccgccgggcc gccgcggagg aggtgcgcgg
     2821 cgacctcttc ggccggggca ccgtcctcga gcaggaggcg tgcagccgta aggtgattct
     2881 ggcggaggcg gctgggcgtg aggtcggcgt ggacgatctc ggcgatgatc gggtggacga
     2941 acgagaggga ggagccgccc tcgaggatgt cggcggctgc cagggcgtcc accgcctggg
     3001 ccgccgaggc atcgtccagc ccggccagac tcgccgcacg cgggagggtc gcccgcaccc
     3061 caaggacggc ggtggccgcc gcgagtcggc cagcggcccc acccagacga ccgagccgta
     3121 cgaggatcgt gtcgtgaacc gcctccggcc gtagttcccg caggcgagcc gcgccggatt
     3181 gggtcggctc aatctcctcg gcacgcgcgg cggcgatcag tccgtggcag aagaaagggt
     3241 tgccggcgac ggcgcgagcc accgcgtcgc agaaagcatc gtcggcctcc gggagttcgg
     3301 atcgaacgag cgatcggatc gagtccgggt cgagcgggtc caccccatga cccaggatcg
     3361 ccgggtgggc acgcagacgg gccaggagat cctccttctc ggggtcgacc ggatctcccg
     3421 tccggacggt gatcaccagc gatgcaggca actcctccag tctcagcacc aggtagtgga
     3481 ggaagcgcag gctctcgggg tcggaccatt ggatgtcgtc gaggagcagg aggagggggc
     3541 tgcgctcggc gaggttggcc gtgagccagt gcaggccatg gaccagcgga aagagatccg
     3601 gaggcccccg gacttccggg gctccgccct caaacagcgg tgcggtcaga ccagcggcac
     3661 cgctcatcag cttctgacga tcggggccga caacggctcc ggcgaagagc tgtatggcca
     3721 ccccccaggg aaagtggcgc tccaactcgt ctccacgagc gctcagcacc cgcaaccctt
     3781 cgagtgcgcc tcggctcgcg gccgctgcta gcaggctcgt cttgccgata ccggcagcgc
     3841 cctcgaacag gaccgcctgc cctgcgccct ggccggcggc gccgatcgca gcgccgatag
     3901 cctggagctg gacgtcacgc tccagcaggt tcgggctgtg cggtgcgtcc cccatcgaaa
     3961 ccgaacctag gctagcgtat cggtcgcggc ggtgttaggt ggaacgatca acttgggccg
     4021 tctccgccaa ctcggtcata tccggcgggg agggtcctcc caagcagatc tcccagaacc
     4081 tgttcgtgac agcacgaacg atcgagacgc attcctctcc ggaccagatc accagccggc
     4141 taggcgaacg cccgtgtcat tcggcggcga ggcaggctcc agcgttgcag aatgttcctg
     4201 ggacggtccg gatgatcccg agtatctcgg ctcgtccacg ctagtgaagc ggaggaaccg
     4261 gtgagcagct tcttctacaa cctggggaag agggccgggc cagcgatccg caagggtcgt
     4321 tgggtgtggt cgtcgctaag cggctcggag gcagaagccc gccgggccga gcgcgccgtg
     4381 ggtcgggagc tggcgggccg gacgaggagt cgcttcgggg tcgacgatga cctccgggac
     4441 agccgtctcc tcgaggagat cggcttctcc ctggtggcga aagacaggaa cgcagatcgc
     4501 gagttcagct tcgaacctct tcggggaggg gggatgaacg ccttcgctct tcctgggggc
     4561 ttcgtcttcg tcacccgagg gcttctcgac gattgcggtc gcgatcgcga cgagctcgcc
     4621 ttcgtcctgg ctcacgagat cggccacgtg gtccggggcc acgcgctcga gcgcttggtg
     4681 ggacggtcag cgctggccgg ggtggtccga gccctccccg cagcggggct gctcggtcgt
     4741 ctggccggcc gaccgagtct cgccctcctc gagagcgcgc attcccggga gcaggaatat
     4801 gaggcggact cggtgggggt ccgcttgtcg atgtcggccg gcttcgatcc ttccgggtcg
     4861 agccgcttcc tcgagcgtct ggttcgaccc gagagagccg tcgtggccaa ttacctagcg
     4921 tcgcatcccg atccagaaca gcggatccgc cgcatcgagg agggacggag ccccggctag
     4981 ttggggcccg gtcgaccctg gcccctcgct ccgggcgcga ggctctcccc gaatatcccg
     5041 cttctgcacc tcgcatgcct aagtccgcga tcctggcggc gacgttcacc gtccaaagta
     5101 gtcacaccct cctgaaaaac gacgggtccg gcatggatcc ccacaagggc gggcggcagg
     5161 gcctcgtgcg caccgccttc catctcgaga acggcaacca cactggcccc gggggccgga
     5221 aagtagaaca tcacgccgcc gccgagccac ttcacgggct gcccccgtat tgtcctctcg
     5281 cccgcgtgga cggggacgga cccggttcga cgcgcggatc gccctcgtcc ttcctggtct
     5341 attggcgaac gtgccggcgg acgaaactgg tgatgctgtc cggaagccat gcggagcagc
     5401 aggtcggccc ctcccttccg caccagtggc cgcgaccggt gccggtgtcc ctcgacgtgc
     5461 tggaagaccg aggtcgacga gttcctcgta ttcgccaagg tcacccgacg tcgccgagga
     5521 ccgcgtgtac ctcactggag tgagctgcag cctatcgcga ggcgtggagt gctccgacgc
     5581 ctaggaccgc gccgtgagcc gcccctaagc actcccattc gtactcgccc ggctgcaggg
     5641 gcccgagctc gacctgcgcg gtctcccccg cctcggcgtg gaggtgctgg ccggcggatg
     5701 cgaggtcgag cgacacc
//
LOCUS       DASUTZ010000141         5549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1724057, whole genome shotgun sequence.
ACCESSION   DASUTZ010000141 DASUTZ010000000
VERSION     DASUTZ010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5549
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724057"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1926)
                     /gene="nuoL"
                     /locus_tag="VF097_06200"
     CDS             complement(<1..1926)
                     /gene="nuoL"
                     /locus_tag="VF097_06200"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX6262422.1"
                     /translation="MIEQAWLIPVIPLTAFFLIAFFGKRTPGQGAGLGILAVGVSWVL
                     SLIVAFQQIGGEEAVERSWPWLEIGSFRFEIGTYVDGLAAMMLTVVTTVSLLVHVYSL
                     GYMRGDRRFTWFYAVLSLFTGSMLNLVLANNLWQLLIGWELVGICSYLLIGHWWEEKE
                     NSSAAIKAFITTKTGDVPFQFGIFALVIGAGTANIQQVTEAVEGGELSSVLILAAALL
                     LFGGAIGKSAQFPLHVWLPDAMAGPTPVSALIHAATMVVAGIYLVGRMFLVFEAAAPE
                     ALTVVAVIAAITMLIAAVLAAVQDDIKRVLAYSTVSQLAYMMAALGVGEAGYTAGFFH
                     LFTHAFFKALLFLAAGSIIHAVHTNNMSEMGGLRRHMPWTFWTFVIGAVALAGIPPLA
                     GFWSKDEIITAAYAEGQIGLWIVALATAALTAFYMTRAVLLTFFGRPRHSEDVHPHES
                     PPVMVGPLVVLAGLSLVIGFVNAPFLGWFSDWVFVHEPHHAEFSFFNAGLSLLIALGA
                     IVLGVALYGRFREREPLRRLGPVYAFAERKYFLDDIYMGGIVEPVKGPIARGVYWTNQ
                     YILDGLVNAAGWIARRLGRGTEAVDRNVVDGIVNRVGVGTGWTGGLLRYVQSGNVQRY
                     AIVLFAGVAILAVIFTRI"
     gene            complement(1931..2239)
                     /gene="nuoK"
                     /locus_tag="VF097_06205"
     CDS             complement(1931..2239)
                     /gene="nuoK"
                     /locus_tag="VF097_06205"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX6262423.1"
                     /translation="MIRLPLVLFFSAGLFSVGVYGVLARRNAVLVLMSIELMLNAVNV
                     NLVAFSQLLQDTMMTGQIFALFVITVAAAEVGIGLAIVILIFRNRETINVDEITLLRW
                     "
     gene            complement(2236..2730)
                     /locus_tag="VF097_06210"
     CDS             complement(2236..2730)
                     /locus_tag="VF097_06210"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX6262424.1"
                     /translation="MTWQEWAFAVLGLVGLGAALRLVTSKNVVHAALYLVVSLLMVGA
                     IYLLLGAEFIGWVQILIYVGAIVVLLLFGLMLTRAPIGREALDNQQRGLAAIVASGIL
                     FGLTFLVQDAFDGQEIEVQPTRIADAGERLFTDFVLPFEVVSFLLLAALIGAIALARK
                     EDAE"
     gene            complement(2727..4025)
                     /locus_tag="VF097_06215"
     CDS             complement(2727..4025)
                     /locus_tag="VF097_06215"
                     /inference="COORDINATES: protein motif:HMM:NF025384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX6262425.1"
                     /translation="MDVREGEARVSPAPAPPDDARHVDVAGERYRRRNPIGAWLLALV
                     TLGLYWVVWQYKVNDEARRYLGDRRIAPALSALAVMIGLAAPVPLIVMLVLALTGTDV
                     SLLMWLLGVGALLIIPIPLIASVFRTGERITLMQERAGLEPSVSPLLGVAVSPAMLHI
                     PLYQSELNRIWGEPRSLPGVGLIKGLFVTLKHLLQPSITQQYPREKPELPARTRGVIA
                     LKPENCTVCWKCSKECPDWCIYIESHKETHEPASGGRARSQKVLDRFAIDFALCMYCG
                     ICVEVCPFDALFWSPEFEYAEYDVLELLHEKDRLRDWTYKVKPPPELEWGAEPEPPEG
                     EEDVAPTSVTPTVQPGEAAPGQTAEAAPQEHAAPPEAPQAPEAPPEQAAPAAREEVEP
                     DQATYDRVLQEQLDAGVNPRVAEGRAKAAAIRAARQGGGG"
     gene            complement(4033..5010)
                     /gene="nuoH"
                     /locus_tag="VF097_06220"
     CDS             complement(4033..5010)
                     /gene="nuoH"
                     /locus_tag="VF097_06220"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX6262426.1"
                     /translation="MSFFDQLFANVWVVLALKVVVVMVFFLTAPMAIGYTEHKVLAHM
                     QHRLGPMEAGRFHGWAQLIADGVKFIQKEDVVPSAADRWVFSLAPAVFMIPVVLVFAV
                     IPFGPDVWAENLDVGLFFVMAISAISVIGVLMAGWSSANKFSLIGALRAAAQLIAYEL
                     PLILAAVAVAMLAGTLSLVGIVEAQRSFWYVLVQPMGLLIFLIASIAELTRPPFDMPI
                     ADSEIIFGAYTEYTGMKFAFFLLAEYAGIVALSAVASVLYLGGYLGPILPAPIWMLLK
                     IGGLSFFIIWLRATYPRLREDQLQRFSWIVLIPLALVNILVTAIVKVVV"
     gene            complement(5007..>5549)
                     /locus_tag="VF097_06225"
     CDS             complement(5007..>5549)
                     /locus_tag="VF097_06225"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018736858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX6262427.1"
                     /translation="RKTSGRSGVASSPPLPARDLIDRLRAQLGDDLTESSDQHGQAAI
                     GVPPARYRETIEWLRDDDELAFDFFDFLTAVDRGDEGFDVVVQLWSTSRRHQARVKVR
                     LPREDPRLPSIHDLFRGADWYERETWELFGIDFEGHPGLVKLVLPEEFEGHPGRKDFP
                     LATRIAKPWPGDEQIGEAEE"
BASE COUNT          982 a         1945 c         1877 g          745 t
ORIGIN      
        1 gatcctcgtg aagatcaccg ccaggatggc gaccccggcg aacagcacga tcgcgtaccg
       61 ctgcacgttc cccgactgga cgtagcggag caggccaccg gtccagccgg tcccgacgcc
      121 cacgcggttc acgatgccgt caacgacgtt ccggtccacg gcctcggtcc cccgccccag
      181 cctccgggcg atccagcccg ccgcgttcac cagcccgtcg aggatgtact gattggtcca
      241 gtagaccccg cgggcgatcg ggcccttcac gggctccacg atcccgccca tgtagatgtc
      301 gtccaggaag tactttcgct ccgcgaacgc gtacacgggg cccagccgcc ggagcggctc
      361 gcgctcgcgg aaccgcccgt agagggccac gccgagcacg atcgctccga gcgcgatcag
      421 gagcgacagc ccggcgttga agaacgagaa ctcggcgtgg tgcggctcgt ggacgaacac
      481 ccagtccgag aaccagccca ggaacggggc gttcacgaac ccgatcacga gcgacaggcc
      541 cgcaagcacc accagcggcc ccaccatgac gggcggggac tcgtggggat ggacatcctc
      601 gctgtggcgc ggccggccga agaaggtgag caggaccgcc cgggtcatgt agaaggcggt
      661 gagcgcggcc gtggccaggg ccacgatcca cagcccgatc tggccctcgg cgtaggcggc
      721 cgtgatgatc tcgtccttgg accagaagcc ggccagcggc gggatcccgg ccagcgcgac
      781 cgccccgatc acgaacgtcc agaacgtcca cggcatgtga cgccggaggc ctcccatctc
      841 gctcatgttg ttcgtgtgga cggcgtggat gatcgagccg gccgccagga acagcagggc
      901 cttgaagaac gcgtgggtga acagatggaa gaacccggcc gtgtagccgg cctctcccac
      961 ccccagcgcc gccatcatgt aggcaagctg ggataccgtg gagtaggcca gtacccgctt
     1021 gatgtcgtcc tggaccgccg ccagcaccgc cgcgatcagc atcgtgatcg ccgcgatcac
     1081 cgccacgacg gtgagggcct cgggtgccgc ggcctcgaag acgaggaaca tccgccccac
     1141 gaggtagatc ccggccacca ccatcgtcgc ggcgtggatc agggccgaca ccggcgtggg
     1201 gcccgccatc gcgtccggaa gccagacgtg gagcgggaac tgcgccgact tcccgatcgc
     1261 gcctccgaac agcagcagcg ccgccgcgag gatcaatacg gaggagagct ccccgccctc
     1321 gaccgcctcg gtgacctgct ggatgttcgc cgtcccggcc ccgatcacga gggcgaagat
     1381 cccgaactgg aacggcacgt cacccgtctt cgtggtgatg aaggctttga tcgcggcgct
     1441 cgagttctcc ttctcctccc accagtggcc gatgaggagg taggagcaga tgccgacgag
     1501 ctcccagccg atcagcagct gccagaggtt gttcgccagg acgaggttga gcatcgatcc
     1561 cgtgaacagg gacagcaccg cgtagaacca ggtgaagcgt cgatcgcccc gcatgtagcc
     1621 cagcgagtac acgtggacga gcagcgagac cgtcgtgacg acggtcagca tcatcgcggc
     1681 gaggccgtcc acgtaggtcc cgatctcgaa ccggaacgac ccgatctcga gccagggcca
     1741 ggaacgctcc acggcctcct cccccccgat ctgctggaag gccacgatca gcgagagtac
     1801 ccaggagacc cccaccgcga ggatcccaag ccccgcgccc tgtcccgggg tccgcttccc
     1861 gaagaaggcg atgaggaaga aggcggtcag ggggatgacc gggatcagcc acgcctgctc
     1921 aatcatcgac tcaccatcgc aggagggtga tctcgtccac gttgatcgtc tcgcggttgc
     1981 ggaagatcag gatcacgatc gccaggccga tccccacctc ggcggcggcc accgtgatca
     2041 cgaagagcgc gaagatctgg ccggtcatca tcgtgtcctg gaggagctga gagaacgcca
     2101 cgaggttgac gttcacggcg ttcagcatca gctcgatcga catcagcacg aggacggcgt
     2161 tgcgccgggc cagcacgccg tagaccccca ccgagaagag gccggccgag aagaagagga
     2221 cgagcggaag ccggatcatt ccgcgtcctc cttccgggcc agcgcgatcg ccccgatcag
     2281 cgcggccagc aggaggaagg agacgacctc gaacgggagg acaaagtccg tgaagagccg
     2341 ctcccccgcg tcagcgatcc tcgtgggctg gacctcgatc tcctgcccgt cgaacgcgtc
     2401 ctgcacgagg aaggtgaggc cgaagaggat ccccgacgcc acgatcgccg ccagcccccg
     2461 ctgctggttg tcgagcgcct cccgcccgat cggcgcgcgg gtgagcatca gcccgaacag
     2521 caggagcacc acgatggcgc ccacgtagat caggatctgg acccacccga tgaactccgc
     2581 gcccaggagg aggtagatcg cccccaccat gaggagcgac acgacgaggt agagggccgc
     2641 gtgcaccacg ttcttcgagg tgacgagccg gagggcggcg cctaggccca cgaggcccag
     2701 gacggcgaag gcccactcct gccaggtcat ccgccgcccc cctgcctggc ggcgcggatc
     2761 gccgccgcct tggcccgccc ctcggccacc cgggggttaa cgccggcgtc gagctgctcc
     2821 tggaggactc ggtcgtacgt ggcctggtcg ggctcgacct cctcgcgggc cgcgggggcc
     2881 gcctgctcgg gcggggcctc cggcgcctgc ggggcctcgg ggggggcggc atgctcctgc
     2941 ggggcggcct cggccgtctg cccgggcgcg gcctccccgg gctgcacggt cggggtcacc
     3001 gacgtcggcg ccacgtcctc ctcgccctcc gggggctcgg gctcggcccc ccactcgagc
     3061 tcgggcggcg gcttcacctt gtaggtccag tcccgcagcc ggtccttctc gtgcagcagc
     3121 tcgaggacgt cgtactccgc gtactcgaac tcgggactcc agaagagcgc gtcgaacggg
     3181 cacacctcga cgcagatccc gcagtacata cagagcgcga agtcgatggc gaaccggtcc
     3241 aggaccttct gcgagcgggc ccggccgccc gacgccggct cgtgcgtctc cttgtgcgac
     3301 tcgatgtaga tgcaccagtc cgggcactcc ttggagcact tccagcagac cgtgcagttc
     3361 tcgggcttga gcgcgatcac gccgcgggtc cgggccggga gctcgggctt ctcccggggg
     3421 tactgctggg tgatcgaggg ctggaggagg tgcttcaggg tcacgaagag gcccttgatg
     3481 agcccgacgc ccggcagcga gcggggctcc ccccagatcc ggttgagctc ggactggtag
     3541 agcgggatgt ggagcatggc cggcgagacg gccaccccca ggagtgggga gacgctcggc
     3601 tcgagccccg cgcgctcctg catcagggtg atgcgctcgc ccgtgcggaa gaccgaggcg
     3661 atcagcggga tggggatgat gagcagggcc cccaccccga gcagccacat cagcaagctc
     3721 acgtcggtcc cggtcagggc gaggacgagc atgacgatca ggggcacggg cgcggccagt
     3781 ccgatcatca cggccagggc tgagagcgcg ggggcgatcc tccggtcccc caggtaccgc
     3841 cgcgcctcgt cgttcacctt gtactgccag acgacccagt agaggccgag cgtgacgagg
     3901 gccagcagcc aggccccgat cggattgcgg cgccggtacc gctcgccggc gacgtcgacg
     3961 tgcctggcgt cgtcgggcgg agcgggcgcg gggctcaccc gcgcctcgcc ttcgcggacg
     4021 tccacggcca cctcagacca ccaccttcac gatcgccgtc accaggatgt tcaccagggc
     4081 cagagggatc agcacgatcc aggagaagcg ctggagctgg tcctcgcgga gccgcgggta
     4141 cgtggcccgg agccagatga tgaagaagga gagccccccg atcttcagca gcatccagat
     4201 cggcgcgggg aggatcggcc cgaggtagcc gcccaggtac agcaccgagg ccaccgccga
     4261 cagcgccacg atccccgcgt actcggccag caggaagaag gcgaacttca tccccgtgta
     4321 ctccgtgtag gccccgaaga tgatctcgga gtccgcgatc ggcatgtcga agggcggtcg
     4381 ggtcagctcc gcgatcgagg cgatcaggaa gatgaggagt cccatcggct ggacgagcac
     4441 gtaccagaag ctgcgctgcg cctccacgat ccccacgagc gagagggtcc ccgccagcat
     4501 cgccaccgcc accgccgcca ggatcagggg caactcgtaa gcgatcagct gggccgcggc
     4561 ccgcagggcc ccgatcagcg agaacttgtt ggccgaggac cagcccgcca tcaggacccc
     4621 gatcacggag atggcggaga tcgccatcac gaagaagagg ccgacgtcga ggttctcggc
     4681 ccacacgtcc ggcccgaagg ggatcacggc gaacacgagg accaccggga tcatgaacac
     4741 ggccggggcc agcgagaaca cccagcggtc cgccgcggag gggacgacgt cctccttctg
     4801 gatgaacttc actccgtcgg cgatcagctg ggcccacccg tggaaccgcc cggcctccat
     4861 cggccccaga cggtgctgca tgtgggccag gaccttgtgc tccgtgtagc cgatcgccat
     4921 cggggcggtc aggaagaaga ccatgaccac gacgaccttg agggcgagca ccacccacac
     4981 gttggcgaag agctggtcga agaagctcac tcctcggcct ccccgatctg ctcgtccccg
     5041 ggccagggct tggcgatccg cgtggcgagg gggaagtcct tcctcccggg gtggccctcg
     5101 aactcctccg gcagcaccag cttgaccagc ccggggtgtc cctcgaagtc gatcccgaag
     5161 agctcccagg tctcgcgctc gtaccagtcg gccccccgga agaggtcgtg gatgctgggg
     5221 aggcgggggt cctcgcgggg caggcgcacc ttcacccgcg cctggtggcg gcgcgacgtc
     5281 gaccagagct ggacgaccac gtcgaatccc tcgtcccccc ggtccaccgc cgtgaggaag
     5341 tcgaagaagt cgaaggcgag ctcgtcgtcg tcccgcagcc actcgatcgt ctcgcggtag
     5401 cgagcgggcg gcacgccgat cgccgcctgc ccgtgctggt ccgaggactc cgtgaggtcg
     5461 tccccgagct gagcccgcag gcggtcgatc aggtcacgtg ctggaagggg cggtgagctt
     5521 gccactccac ttctcccgga tgtcttcct
//
LOCUS       DASUTZ010000142        10621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_428891, whole genome shotgun sequence.
ACCESSION   DASUTZ010000142 DASUTZ010000000
VERSION     DASUTZ010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10621
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428891"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..565
                     /locus_tag="VF097_06230"
     CDS             146..565
                     /locus_tag="VF097_06230"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HEX6262428.1"
                     /translation="MSFETLGDVNYLAVLVATVAWALLGAIWYAPPVLGRASFRASGW
                     DMPERPPSPIYLGASLILYFLAALATAWIARETGSETLVDGLVLGVAVGIGYMMVLVA
                     VTAMFERKPHPAAWFWINAPFSVLGLLIVGVIVSVWD"
     gene            745..1347
                     /locus_tag="VF097_06235"
     CDS             745..1347
                     /locus_tag="VF097_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262429.1"
                     /translation="MTLLAPSALAADGLVRTTWPSAEDPGLPFYARLGGAAPPLFYQD
                     GEWAAVVFYRDPGCVREDFNLLLQFDVPGAFACPHTVSGFHLWHGEAFNGSPKAATIT
                     GTGVPVWFAPVDAVSQAVQDGVLTIGELASLEGLVVGHADRFNEAIHPHPLPPELGGG
                     GHATPKLTLNAHGRLEDGRRFDLHIAQVFPEIHALRIEFR"
     gene            1559..2590
                     /locus_tag="VF097_06240"
     CDS             1559..2590
                     /locus_tag="VF097_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262430.1"
                     /translation="MLVLITAACGGVDPAETAPEPNVERTTYSVGVDGTADDFTGSFH
                     KFFPRMLTVHPGDTIVFQRPENGEPHTVTLGTDAPTSESLPGGNFFSGGFGGPPLLKA
                     AMPCFLTEGIPASEGCSSDQQERVPFDGKQSWYNSGGLLGDEEFTLQLAESIAPGSYP
                     FVCLAHPIPMRGQIQVVGPGEPADDPADVIARADEEMQREAEAIRSRVEAPPSVSEGE
                     VDAAIFGGLSASVWANMFFPEEVEIPVGGTVTWNVRHIHTIAFNAPESASPFYERADD
                     GSVQENEEGAEEQGDFAGWDGTGFLNSGLLEGFQPPGQFSVTFDTPGTYSYQCLVHFD
                     MEGRVKVSG"
     gene            2826..6956
                     /locus_tag="VF097_06245"
     CDS             2826..6956
                     /locus_tag="VF097_06245"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX6262431.1"
                     /translation="MGAHEHGEGLTLVAQEGESVEPGGRCADAAPTRDYDVAAIAVEI
                     TLNRYLDHDPEGRMYVLEEDLRAVREEEEANQRARTTGSEAAVSIGLQGDAIQPLTLR
                     VLPGECLRIHLRNDLPGEEPATFHVHGSQLLVAESGRPAIATEPSAVARPGQTVTYEW
                     AVPADEPEGTHHVHSHGDTRRQTAHGLFGAVIVEPAGSTWTDALTGEETTAWAAIVTP
                     PDGPAFREFVSYYHEIGTEAEVASGADGSLLPLVDPHTGSYKPSGRALNYRSEPFMHR
                     LQLQVEEGLPADESIAYSSYAFGDPATPVMRTYVGEPVKQRVVHAGSEVFHVHHVHGG
                     SIRWPRQPGLDESPAITHLEKNPPLLPAVTERTDSQSIGPSEAFDVEPECGAGGCQQS
                     VGDFLYHCHVAHHYISGMWGIWRVYNTLQDGDASTDRMPPLPELPDRPGTMRPAVTSD
                     ELPPELARRIEELLPPRGTPAEGDASVWDWSLEGGLYRGEPEDQRSWPGHIPATSGRP
                     PLLFDPRDGRLAYPHLRPHLGRRPPFAPGHGPAPYLDPASGTPTVPRPGENGDRSVCP
                     SGTRVRDLDIIAIDAPISLNTEENLVDPAGMLFVRREQRDGVLADPAERVPLVLRANA
                     SVDCVDVTLTSEVADDADNDWHSKVNAHIHFVQFDVQGSDGVDAGFNYEQSVRPWTVD
                     SERISGVTRQGAVRVPVRRTDRFHPGALVMVGVDTRRRAEVRTVKAVQDRAVVLAEPL
                     DHHHGRGERITMEFVRYRWYPDVQFGTAFFHDHVNAIFSGRHGLWGALVSEPPDATWH
                     DPETGEELLSGPVADIHTTQPVSLEAAGSFRELVAFLQDDNQVTKEGRSSGSSLGLRV
                     EPLDPRSEGDPAAAFDSTRHGDPETPILRAHPGDPLVVRTLVSAANDVHTIHLDGHAF
                     RSESWSVTSPPVAAAHLGISERLDLVVPSAGGPARLPGDYLLRNGRASKLREGSWGLV
                     RVVPRGDEDAPQSLPGVGRGPPGGEICPPDAPVRSFDITAVEAPLPMLEGRLGVAFVP
                     THAAPEISSGGRPAEPLVARATVGDCLEVGVRNATGRVVSLEPDLMVFDPGERAVGRN
                     GGEGIPPGGHRISRFYAHPDFGEATVLLLDGGDPLGGPVSGAYGAVAVAEPGATFRDP
                     RTGEEVTTGWKVDVVRPEGRSYRDAVLFFHEEDAAIGTHLMPYRDAVDGVVGINYRFA
                     SLEDRLFEDPDPGEVFNSATHGDPPTPIIEAYAGDPLRLRVLAPVSEQGQVFSVEAHR
                     WRQEPRPDAILLSSIQLGGLEAVTIDLLDGAGGPARSPGDYLYGDHREPYREAGLWGL
                     LRVRDPCAVDQGLRPLHAAPSECRAVLPGVMAGFLGAAGILALGVGARYLFRRRPK"
     gene            7212..8723
                     /locus_tag="VF097_06250"
     CDS             7212..8723
                     /locus_tag="VF097_06250"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012947257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX6262432.1"
                     /translation="MSIRTRVLSGTTAVLVGMGGLALPSGAIAAPGSPGEPRFTAGAP
                     GAGDAYFPFSGNGGYDVGHYDLTLTYTPPEPAPAPIVGQLDGMATIDIVATQDLDRFN
                     LDLRGLDVHAVTVNGKPAAEVLPPAPGAEVEGAAYWQVQDDAARIWELTIQPRPKLKE
                     GQAARVVISYGGETGRPRDIEGVLYGWVTTRDGAMVVGEPEGTMTWYPVSDHPTDKAT
                     YSFEITVPEEKVAVANGLPARDPVTDDGWTTWYWDAPDAQASYLTTASVGDYELRFSE
                     AADGLPIIDAVDDNLTAGNLVTSNASLALQPAMIAFFEETFLTYPFNSYGSIVDDDSV
                     GYALETQTRPVYSRVAREGTVAHELAHQWFGNAVSPQRWQDIWLNEGWATYGEWLWSE
                     HAGRDTAQESFEDVMSIPAGSSFWNHVVADPGPFGLFHPANYDRGAATLHALRLEVGD
                     EAFFDAAREWLTRHDDGTGTTEDFQAIHEEVSGQDLDGFFDTWLRTPSKPPFP"
     gene            complement(8874..9593)
                     /locus_tag="VF097_06255"
     CDS             complement(8874..9593)
                     /locus_tag="VF097_06255"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX6262433.1"
                     /translation="MSSFFYNLGKRAGPVFRKGRWVWSSLTGSEAEARRAERAVGREL
                     AGRMRDRLGIDDDLQDRRLVEEIGFSLVAIERSADREFSFEPLRGGGANAFALPGGFI
                     FVTRALLDDCGRDRDELAFVLAHEIGHVVQGHALERLVGRSALSGVVRALPAAGLLGR
                     LAGRPSLALLESAHSREQEYEADSVGVRLSMAAGFEPSGSSRFLERLVRPEGAVVAAY
                     LASHPHPEARIRRIKQARRRS"
     gene            9817..10191
                     /locus_tag="VF097_06260"
     CDS             9817..10191
                     /locus_tag="VF097_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262434.1"
                     /translation="MAKQELADFLTLVQRQQALLDELTPIPETDARALFARLGIDEPN
                     RYEDAVTAFSSFRQYAMTAEGASVEVVYLKRIDASSGNDVLYVHLDDVPELGFVVCRL
                     VEGGFLGRYKTGSGLREALGIW"
     gene            complement(10225..>10621)
                     /locus_tag="VF097_06265"
     CDS             complement(10225..>10621)
                     /locus_tag="VF097_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262435.1"
                     /translation="LAAAAGGGTRLGPWDLPVDVLVGAAVLVGLGLYGSLPWPLVIVV
                     LVVFGGAFILLRNPFPGMVLQAVAWGGILWRFWADRVAAGWIPVAVAGVIGIAERRRF
                     VHVVLPAFFRGAAQTLTLRRGRDYRMPDD"
BASE COUNT         1680 a         3644 c         3648 g         1649 t
ORIGIN      
        1 ttccagaaca cggtcggagg tcacgcacac ttcgccgggc cgggagcgag tggctgcgat
       61 gacgtgggga tggagagcct cgaggcgtgc ctcgtcgagg ccctcttcgg ttagcacgag
      121 gtcgatcggg cggaaggaga tgtagatgtc gttcgagacc cttggcgatg tgaattacct
      181 ggcggtgctg gtcgccacgg tggcctgggc gctcttgggc gcgatctggt acgcgccacc
      241 ggtgctgggc cgggcctcat tccgagcctc aggatgggac atgcccgagc gaccaccttc
      301 ccccatctac cttggggctt cgctcatcct ctacttcctc gccgcgttgg cgaccgcgtg
      361 gatcgcccgt gagacgggct cggagacgtt ggtcgacggc ctcgtacttg gggtcgccgt
      421 tggcatcggg tacatgatgg tgctcgtcgc agtgaccgcc atgttcgagc ggaagcctca
      481 tccggccgcc tggttctgga tcaacgcacc gttctccgtc ctggggctcc tcatcgtggg
      541 ggtgatcgtc agcgtctggg attgaggatc aggcagccca cgaacgtctt cggcaaacgg
      601 gaagtgccca ccgtgaggcg aacaccgtcc ccgtaactcg gcgagacgct agggagggag
      661 gatgctatgc aaacgcagcc tcaaagggag catcgaccca tcgcgggacc tatcgccatc
      721 ctggctctgg tcccgatcat cgtcatgact ttgctggcac cgtccgctct ggcagccgac
      781 ggcctggttc gcacgacgtg gccctcggcc gaggaccctg gcctcccgtt ctacgcgcgg
      841 ctgggcgggg ccgccccgcc gctcttctat caggacggcg agtgggccgc ggtcgttttc
      901 taccgagatc ccgggtgcgt gcgcgaggac ttcaacctgc tgctgcaatt cgacgttccg
      961 ggggcgttcg cctgcccgca cacagtcagc ggattccatc tctggcacgg cgaggcattc
     1021 aacggctctc cgaaggctgc gaccatcacc ggcaccgggg tcccagtctg gttcgctccc
     1081 gtggatgccg tcagccaggc ggtccaggac ggtgtcctga cgatcggcga gctggcgagc
     1141 ctggagggac tggtggtcgg ccacgccgat cgattcaacg aggcgattca tccgcatccc
     1201 ctcccgcccg agctgggcgg cggcggacat gccaccccta agctgaccct gaacgcccac
     1261 gggcggctgg aggacggacg ccgcttcgac ctgcatatcg cgcaggtctt tcctgagatc
     1321 cacgctcttc ggatcgagtt ccggtgatgg ctgcatcacc ggaggatccc ggtcgcctgg
     1381 ttctgggtca acgcaccgat ctctcttgtc gggctgctca tcgtgggggt gatcgtcagc
     1441 gtctgcgact aagactctcg acgttcacca cggggagatc gccatgaaat ggtcagcatt
     1501 cgccacatcg acgtggcacc cgagtcttcg ccagagccgc ttgggaggag gcctcgccgt
     1561 gcttgtgctc atcacggcgg cctgtggagg agtggatccc gcggagacgg cgccggagcc
     1621 gaacgtggaa cggaccacgt actccgtagg cgtcgatgga acggcggatg atttcaccgg
     1681 cagcttccac aagttcttcc cccggatgct taccgtgcat ccgggcgaca ccatcgtctt
     1741 ccagcgcccg gagaacgggg agccccacac ggtcaccctg gggacggatg ctccgacgag
     1801 tgagtccctc cccggaggca acttcttcag tggcggattc ggagggcccc cgctcctgaa
     1861 ggccgccatg ccgtgctttc tcaccgaagg catcccggcg tcggagggct gctcctccga
     1921 tcagcaggag cgggtgccct tcgacgggaa gcagtcctgg tacaacagcg ggggtctgct
     1981 cggggacgag gagttcaccc tccagcttgc cgagagcatc gcgcccggca gctacccgtt
     2041 cgtctgtctg gcgcacccga tccccatgag aggacagatc caggtcgtgg ggccgggtga
     2101 gcccgccgat gaccccgctg acgtgatcgc ccgagccgat gaggagatgc agcgagaagc
     2161 ggaggccatc aggtcccgcg tggaggctcc accatccgtc tcggagggtg aggtcgatgc
     2221 ggcgatcttc ggcggactga gcgcctccgt ctgggccaac atgttcttcc cggaggaggt
     2281 cgagatccca gtcggaggca cggtgacgtg gaacgttcga cacatccaca ccatcgcctt
     2341 caacgccccc gagagcgcct cgcccttcta cgagcgcgcc gacgacggaa gcgtgcagga
     2401 gaacgaggaa ggggctgagg aacagggcga cttcgccggg tgggatggga ccggcttcct
     2461 caattcggga ctcctcgagg gcttccagcc accggggcag ttcagcgtga ccttcgacac
     2521 cccaggcacg tattcatacc aatgcctggt gcacttcgac atggaaggaa gggtcaaggt
     2581 gagtgggtga agctcggcct gcctccgaac ctccccagcc actgccggat cggctaagac
     2641 gcgccacctc gcacccgatc gtgcgagcgt cggcggagct cgtggcggtc ctcctcaccg
     2701 ccctggccct ggcctccgca acctcgaacg cctcccccgg agagccgctt cgcgtcaaag
     2761 gccgggccgc gcagacggat cccggccgtg gagccggcga tcgacacgct cacgggaaag
     2821 ggcccatggg agcacacgag cacggggagg gtctcacgct cgtcgcgcaa gaaggcgagt
     2881 cggtggagcc gggagggcgc tgcgcggatg cagcaccgac ccgtgactac gacgtcgcgg
     2941 cgatcgcggt ggagatcacc ctcaaccgct acctggatca cgacccggag gggcgcatgt
     3001 acgtcctcga ggaggatctg agggcggttc gcgaagagga ggaggcgaac cagcgggctc
     3061 gaacgacggg atccgaggcg gccgtctcca tcggtctcca gggcgacgcg atccagcccc
     3121 tgaccctccg ggttctcccg ggcgaatgcc tgcggatcca cctgcgcaac gatctcccgg
     3181 gagaggagcc ggccacgttc cacgtccacg gctctcagct cctggtggcc gagtccggcc
     3241 gtccggcgat cgcgacggag ccctccgcgg tcgcccggcc ggggcagacg gtcacctacg
     3301 agtgggcggt ccctgcagac gagcccgagg gcacccacca cgtccacagc cacggcgaca
     3361 cccggcgtca gaccgcgcac ggcctgttcg gtgccgtgat cgtcgagccg gcgggctcca
     3421 cctggaccga tgccctcacg ggagaggaga cgaccgcgtg ggcggcgatc gtgacccctc
     3481 cggacggtcc ggccttccgc gagttcgtca gctactacca cgagatcggg acggaggccg
     3541 aggtcgcatc gggggcggac ggctcactgc tcccgttggt ggatccgcac acgggctcct
     3601 acaagccgtc gggacgtgcg ctcaactacc ggagcgagcc cttcatgcac cgactgcagc
     3661 tccaggtgga ggagggtctc cccgccgacg agtcgatcgc gtacagctcc tatgctttcg
     3721 gtgaccccgc gactcccgtc atgcgtacct acgtcggcga acccgtcaag cagcgggtgg
     3781 tccacgccgg gtcggaggtc ttccacgtcc atcacgtcca cggcggctcg atccgctggc
     3841 cgcgacagcc gggcctggac gagagcccag ccatcaccca tcttgagaag aacccaccgc
     3901 ttctcccggc ggtgaccgag cggaccgact cgcagtcgat cgggccatcc gaggccttcg
     3961 acgtcgagcc cgagtgcggg gccggcgggt gccagcagag cgtcggagac ttcctgtacc
     4021 actgtcatgt cgcgcatcac tacatctcgg ggatgtgggg gatctggcgc gtctacaaca
     4081 cgctgcagga cggcgacgcg tcgacggacc ggatgccccc gctgccggag ctgcccgacc
     4141 gcccgggcac catgcgaccc gcggtcacct cagacgagct tcctccggag ttggcgaggc
     4201 gcatcgagga actgctccct ccgcggggca cccccgccga gggagacgcg tcggtctggg
     4261 actggtccct ggaaggcggc ctctatcggg gcgagcccga ggaccagagg tcgtggcccg
     4321 gccacatccc cgccacgtca ggacggccac cccttctctt cgacccgcgg gacggccggc
     4381 tcgcctatcc ccacctgcgt ccgcatctcg ggcggcggcc gcctttcgct ccgggtcacg
     4441 gccccgcccc ctacctcgat ccggcgagcg gcacgccgac cgtgcccaga cccggtgaga
     4501 acggggatcg aagcgtctgt ccctcgggga ccagggttcg tgacctcgac atcatcgcga
     4561 tcgacgcgcc gatctctctc aacacggagg agaacctggt cgatccggcc gggatgctct
     4621 tcgtcagacg cgagcagcgt gacggcgtgc tggccgatcc agcggagcgc gtgccgctgg
     4681 tgctgcgggc gaacgcatcg gtcgactgcg tggacgtgac gctcacgagc gaggtggcgg
     4741 acgacgccga caacgactgg cactccaagg tgaacgctca catccacttc gtgcagttcg
     4801 acgtgcaggg cagcgacggt gtggatgccg gcttcaacta cgagcagtcc gtccgcccct
     4861 ggaccgtcga cagcgagcgg atcagcgggg tgacgcgcca aggagccgtc cgggttcccg
     4921 tgagacggac cgatcggttc catccgggcg cgctcgtgat ggtcggcgtc gacacccgga
     4981 ggcgcgcgga ggtccgaacg gtgaaggccg tgcaggatcg agcggtcgtc ctggccgagc
     5041 ctctcgacca ccatcatgga cggggcgagc ggatcacgat ggagttcgtc cgctaccgct
     5101 ggtacccgga cgtccagttc gggaccgcct tcttccacga ccacgtgaac gccatcttct
     5161 ccggccgcca tgggctctgg ggcgcgctcg tctcggagcc gccggatgcc acctggcatg
     5221 acccggagac gggagaggag ctgctctcgg gcccggtcgc cgacatccat accacccagc
     5281 ccgtctcgct ggaagcggcc ggcagcttcc gtgagcttgt cgcgttcctg caggacgaca
     5341 atcaggtcac caaggaaggc cgctcatcgg ggagctccct cggacttcgc gtggagcccc
     5401 tcgatccgag gtcggagggg gatccggccg cggcgttcga ttcgacgcgc cacggcgacc
     5461 ccgagacgcc gatcctccgg gcccatcccg gtgaccccct cgtcgtgcgg accctggtgt
     5521 cggcggccaa cgacgtacac acgatccacc tggacggtca cgccttccgg tcggagtcat
     5581 ggtccgtgac atccccgccg gtggcggccg cgcacctggg catctccgag cggctcgacc
     5641 tcgtcgtccc ctccgcgggc ggtccggcgc ggctgcccgg cgactacctg ctgcgcaacg
     5701 gccgcgcatc gaagctgagg gaggggtcgt ggggcttggt gcgggtggtc ccccgtgggg
     5761 acgaggatgc gccgcagtcg ctccccggcg tggggcgtgg tccgcccggg ggcgaaatct
     5821 gcccacccga tgccccggtg agatcgttcg acatcacagc agtggaggcc cctctgccga
     5881 tgctggaggg gcgcctcggt gtggcgttcg tgcccacgca cgcggcgccc gagatctctt
     5941 caggaggacg cccggcggag cctctggtgg ctcgggcgac cgtgggcgac tgcctcgagg
     6001 ttggcgtccg caacgcgacc ggccgggtcg tttccctgga gccggatctc atggtcttcg
     6061 acccggggga gagggccgtg ggccgaaacg gtggggaagg gatcccgccc ggaggccatc
     6121 gcatctcccg cttctacgct cacccggact tcggggaggc caccgtactg ctcctcgatg
     6181 ggggcgaccc gctcgggggg cctgtctcgg gagcgtacgg cgcggtcgcg gtggccgagc
     6241 ccggtgcaac gttccgggac cccagaaccg gtgaagaggt gacgacgggg tggaaggtgg
     6301 acgtcgtccg tcccgaaggg cggtcctacc gggacgccgt gctgttcttc catgaggagg
     6361 atgcggcgat cgggacccac ctgatgccct accgcgacgc cgtcgatggg gtggtgggca
     6421 tcaactaccg gttcgcctcg ctggaagaca ggctgttcga ggaccccgat ccgggagagg
     6481 tgttcaacag tgccacgcat ggggatccgc cgacgccgat catcgaggcc tacgcgggcg
     6541 atccgctccg cctgcgtgtg ctcgcccccg tgagcgagca ggggcaggtc ttcagcgtcg
     6601 aggcccaccg ctggaggcag gagccgcggc ccgacgcaat actgctgagc tcgatccagc
     6661 tcggcgggct ggaggcggtc accatcgatc ttctagacgg ggccggggga ccggctcgat
     6721 cgccgggcga ctacctgtac ggcgaccatc gtgagccgta ccgggaggcg ggactctggg
     6781 ggcttctgag ggtccgagac ccctgcgccg ttgaccaggg tctccggcca ctgcacgcgg
     6841 ctccgtcgga gtgccgcgcg gtccttcctg gagtcatggc cggattcctc ggagccgcgg
     6901 ggatcctcgc gctgggcgtc ggcgcgagat acctcttccg tcgccggccg aagtgacggt
     6961 atgaagagct cgaggaaagt gccccgccga tctgctgttc gtacgtcgaa ccgagccggt
     7021 cgttgccaca tacgccggaa cgcgatggtc tggttcacgg ccgcatcctt cccgccgaag
     7081 gtgggcgacc atgccccgat gtcgcacccc ttgtggtggg ggcgccgggc gccaggctga
     7141 cgctttagca ggcgtcgcgc tgatggcggg ttgtcctata gtgcggatac ggtgagtgga
     7201 ggaggctgat cgtgtcgatt cgcacgcgcg tactgagcgg gacgacggct gtgctcgtgg
     7261 ggatgggggg tctcgccctt ccgagcggcg cgatagccgc tcctggctcg ccgggcgagc
     7321 cgcggttcac cgccggtgct cccggcgccg gcgacgccta cttcccgttc tccgggaacg
     7381 gcggctacga cgtcgggcac tacgacctga cgctcaccta caccccgccg gagcctgcgc
     7441 cggcgccgat cgtcggccag ctagacggaa tggcgacgat cgacatcgtt gccacccagg
     7501 acctggaccg gttcaacctg gacctgcgag gtctggacgt gcacgccgtc acggtcaacg
     7561 gcaagcccgc cgcagaggtg ttgccgccgg ctccgggtgc cgaggtcgaa ggggccgcct
     7621 actggcaggt ccaggacgac gcggcacgga tctgggagct gacgatccag ccccgtccca
     7681 agctcaagga aggacaggct gcgcgggtcg tgatctcgta cggcggtgag accggtcggc
     7741 cgagagacat cgaaggcgtc ctctacggat gggtgaccac ccgggacggc gccatggtgg
     7801 tcggggagcc cgaggggacg atgacctggt acccggtcag cgaccacccg accgacaagg
     7861 cgacctacag cttcgagatc accgtgcccg aggaaaaggt cgcggtcgcc aacggtctgc
     7921 ccgcgcggga tccggtgaca gacgacggct ggaccacctg gtactgggac gcgccggacg
     7981 ctcaggccag ctacctgacc acggcctcgg tcggtgacta cgagctgcgg ttctcggagg
     8041 cggccgacgg gctgcccatc atcgacgccg tggacgacaa cctgacggcc ggcaatctgg
     8101 tcacctctaa cgccagcctc gccctccagc cggccatgat cgccttcttc gaggagacct
     8161 tcctcacgta cccgttcaac tcgtatgggt cgatcgtgga tgacgactcg gtcggctacg
     8221 cgctggagac gcagacgagg cccgtgtact cgcgcgtggc ccgtgagggc accgtcgccc
     8281 acgagcttgc ccatcagtgg ttcggcaacg ccgtgagccc tcagcgctgg caggacatct
     8341 ggctcaacga gggctgggcc acctacggcg agtggctgtg gagcgagcac gcggggcgcg
     8401 acacggcgca ggagagcttc gaggacgtca tgtccatccc cgccggcagc tccttctgga
     8461 accacgtcgt cgcggaccca ggtccgttcg ggctgttcca cccggcgaac tacgatcgcg
     8521 gcgccgcgac cctgcacgcc ctgcggctcg aggtcggtga cgaggcgttc ttcgacgccg
     8581 ctcgtgagtg gctgaccaga cacgacgacg gtaccgggac gacggaggac ttccaggcga
     8641 tccacgagga ggtctcgggc caggatctcg acgggttctt cgacacctgg ctgcggacgc
     8701 cgagcaagcc cccgttcccc tgagcccgct cgagccgctg gcctgactcc gccccggcct
     8761 agggaaacgg aggcggagat gccgccgccc caggccgatc ccggcgggtg atcgaggagc
     8821 gtgggggccc gctggtcccg atctggccgc gagaccccgc cgaccagggt tgactagctg
     8881 cggcgccgtg cttgcttgat gcgccggatc cgcgcttcgg gatggggatg ggacgcgagg
     8941 taagcggcga cgacggctcc ctcgggtcga accagacgct ccaggaagcg gctcgatccg
     9001 gaaggttcga agcccgccgc catcgacaag cggaccccca ccgagtccgc ctcgtattcc
     9061 tgctcccggg agtgcgcgct ctcgaggagg gcgagactcg gtcgacctgc caggcgaccg
     9121 agcagccccg ctgcgggaag ggctcggacc accccggaca gcgctgaccg tcccaccaat
     9181 cgctcaagcg cgtggccctg gaccacgtgg ccgatctcgt gagccaggac gaaggcgagc
     9241 tcgtcgcgat cgcgaccgca atcatcgaga agtgctcggg tgacgaagat gaagccccca
     9301 ggaagggcga aggcgttcgc gccccctccc cgaagaggtt cgaagctgaa ctcgcgatct
     9361 gcgcttctct ctatcgccac cagggagaag ccgatctcct cgacgagacg tctgtcctga
     9421 aggtcatcgt cgatcccgag gcgatccctc atccggcccg ccagctcccg acccacggcg
     9481 cgctccgctc ggcgggcctc cgcctcggag ccggtcagcg acgaccacac ccagcgtccc
     9541 tttcggaaca ccggcccggc cctcttcccg aggttgtaga agaagctgct cactcgttcc
     9601 tccgctccaa cgtggacgag cccgggatcc atccgggccg tcgcggacca ttctccacgc
     9661 ttgcgtcaag cctgcctcgg tgcccggccg ctttctcgtg ctctctcccg ggccagaatc
     9721 gcgtccaggg catcagggca gagaggatcg gggccttccg gccctacccg gcccccgagc
     9781 gccgtgcgac cgttctccag gaggtgtgac atgtccatgg ccaagcagga actcgccgac
     9841 ttcttgacgc tggtgcagcg gcaacaggct ctgctcgatg agctcacccc gatcccggag
     9901 acggatgccc gggccctatt cgctcggctg gggatcgacg aaccgaaccg ctacgaggac
     9961 gccgtgaccg ccttcagcag cttccggcag tacgcgatga ccgccgaagg cgcgtccgtg
    10021 gaggtggtct acctgaagcg gatcgatgcc tcctcgggga acgacgttct ctacgtgcac
    10081 ctggacgacg tgcccgagct cggcttcgtc gtctgccgtc tggtcgaggg aggcttcctg
    10141 ggtcgctaca agacgggatc ggggctgagg gaggcgctcg ggatctggta ggcggctcgt
    10201 cggccgtctt cggggtccgt ctcgtcagtc gtccggcatc cggtagtcgc gccctcgccg
    10261 gagcgtcagg gtctgcgcgg cgccgcggaa gaacgccggc agaactacat gcacgaaccg
    10321 gcggcgctcg gcgatgccga tgacgccggc caccgccacg gggatccatc ccgcggccac
    10381 ccggtcggcc cagaaccgcc acaggatccc tccccaggcc accgcctgga ggaccatgcc
    10441 cgggaacgga ttccgcagga ggatgaacgc accgccgaac acgaccagca cgacgatgac
    10501 gaggggccag gggagcgagc cgtagagccc gagcccgacc agcacggcgg cccccacgag
    10561 cacgtcgacg gggaggtccc agggtcccag acgggttccg ccgccggccg cggcggccag
    10621 c
//
LOCUS       DASUTZ010000143        16669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_819037, whole genome shotgun sequence.
ACCESSION   DASUTZ010000143 DASUTZ010000000
VERSION     DASUTZ010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 16669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16669
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819037"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1144
                     /locus_tag="VF097_06270"
     CDS             38..1144
                     /locus_tag="VF097_06270"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017591470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX6262436.1"
                     /translation="MLSADDALAGRLQALVDRLASRKRIPHAILGLASGDGPLRWIGA
                     SGPARPDGTPMRPDTPYMLASVTKLYIGTTILQLYERGEVDLDAPITAYLAVDRVAGL
                     HRLGGVDHTSRITVRHLLGHTSGLPDYLEDRPSDGRRIYEEIEDGVDRSWTFDDVIEI
                     ARGMRPRFEPQDLSAGRQKARYSDTGFQLLLMIIESVAGRPFYEVFEEGLFRPFGLRH
                     TWVPGRSEPLDPPPDDPAAVWAKGRPIHIPKAMASTNDLMGTADDTLRFMGALTRGEV
                     FQDPATYRLMHERWNRIFYPMRYGLATMRYRVPKLFGPGRRAVTLVGHSGTTGSWLLH
                     CPELDLVLTGTVDEASARAVPFRFLPRVLKALQG"
     gene            1394..1885
                     /locus_tag="VF097_06275"
     CDS             1394..1885
                     /locus_tag="VF097_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262437.1"
                     /translation="MRRVLALTLFLGILVASAPASAEKPVREFLPAEDEVISDVCDFD
                     LGVVVLENNELLTTFFDQEGNVTKQLVTGSLVVELRNEETGVSLVANIPGPGVITFPG
                     DSELVTALGPWLWLFFPGDLGPGEPGMALLTHGRFVVEFAPDGVTIVERRGRQVDVCA
                     LLS"
     gene            complement(2048..2662)
                     /locus_tag="VF097_06280"
     CDS             complement(2048..2662)
                     /locus_tag="VF097_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262438.1"
                     /translation="MRVAIVAVATLTALGALAYAGAQPGSPETSPEELSESVEPEEPE
                     SLTGEEDDGFDPAPLGEGSGATEHASRTAIDVLTMIQAWVDGELGFGCEFGQVVSAAA
                     RAEEEDGDDHDPCERNGSSGEGASAAGKVKAEEMSAAGKAKGRPAAEAGKAKGAEASA
                     AGKAERGDAPGRGASNGRGGGGSGPTSDPGLPPGAGPGGTPGGP"
     gene            2825..4339
                     /locus_tag="VF097_06285"
     CDS             2825..4339
                     /locus_tag="VF097_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006621280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSLREA domain-containing protein"
                     /protein_id="HEX6262439.1"
                     /translation="MRRIALLPALTLLISLTLPAAPSAGTTVIHVTTTDDEIANDGDC
                     SLREALSAAATDAPRDACPAGSGGDVVRLPAGVFVLTIPPDGTPDDGEDGDLDIFDQV
                     TIRGAGARRTILDGNGTDRVLHVGPMSVVSVMDLTIRDGWTDDAFGGGAILNIGATLT
                     LRRVTVKGSEATAGDGGGISTSNAGAVLTVVDGSVIDNHTTAFGGNGGGIANVNASVA
                     NLVRVTVSGNSAFQQGGGIANEAGSTIDLTSVTITDNVANADEGGAEGTGGGIQHSGV
                     AATLRRTLVAGNRDLDPNLGAHDPDCSSIGSFTSEGHNLVGEPSAGCNFPAAGSDRMG
                     EHPMLRPLRNNGGPTDTHALRRDSPAVDAIPGAACDPRDQRGAPRSGRCDIGAYELVR
                     CRGAIVNRVGTGGKDRLRGTAKRDGFLGLGGGDTIRGRKGNDGICGGPGKDVLIGGAG
                     RDVLVGEKGPDTLRGGGGRDLLLGGPGSDALRGGAGRDRCRQGKGSGPVSGCER"
     gene            complement(4320..5159)
                     /locus_tag="VF097_06290"
     CDS             complement(4320..5159)
                     /locus_tag="VF097_06290"
                     /inference="COORDINATES: protein motif:HMM:NF015597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEX6262440.1"
                     /translation="MTVLGPGAHGVVIATPDPRSAAMDRLTLRSAGIPAALVEDLGLA
                     LVAEPEGDDPLAEAAAAWAVVTRRPAVSRQGDRLAVGRPDGSVEVSEPATFSSELLQG
                     GPAPRRAAVFGGAWIEEGEPEYADAVELGTRAAGAGIHLVCGGYGGIMEAVSRGAAGA
                     GGAAIGISIAAWVGRVTPNPWLTHVVSARDLLARFPLLLDADVWVAFAGGVGTLAEVA
                     LCWNLMQMSVEPRPLLLVGERWGTLVEAFRRELIVTESGHHDLIRVCDVDEAAEALTA
                     RSR"
     gene            complement(5156..6577)
                     /gene="thiI"
                     /locus_tag="VF097_06295"
     CDS             complement(5156..6577)
                     /gene="thiI"
                     /locus_tag="VF097_06295"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HEX6262441.1"
                     /translation="MARLMLKTAGEIGVKSPRTRRRFLRVLRRNVRRSLDRAGVQASV
                     QAGWGRIMVRTSDPEAATEVLSRVFGLHSVSLVETVTFDGLDDLVAKAAERSRDRIRG
                     RTFAIRPRRSGEHDFRSHDVAVKLGAALLEDSAGVNLDEPEVEVPVEIVNDDAHLVLE
                     ATKGAGGLPLGTGGRALAMFSGGFDSPVATWMTMSRGTSVELVVFDLGGCAQTDGALT
                     VAKALVDRWARGLEPVAHIVELLPVVAALTQRVEGRLRQLVLKRAMYRAATILADERQ
                     VDAVVTGESIGQASTQTLRNLSVVERATTYPVLRPLVGMSKEEIMGRAREIGTHEASA
                     RVHEYCSIATGPVETAARFEEVADADGEIDESVLRAAVEKRRVVELATWTPGPLPEYV
                     LEQAPDGVIVIDVREEGEGPVVGDRRLPFSRLHEWLPQLDPKEPYLFVCSVGSRSELV
                     AEQLWGKGYRAYCLAGGAHRLRR"
     gene            complement(6593..7048)
                     /locus_tag="VF097_06300"
     CDS             complement(6593..7048)
                     /locus_tag="VF097_06300"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEX6262442.1"
                     /translation="MATPSKPKRPRDELGRPLPWDAPNRLPMEDFDQLPLEENHRLAI
                     AYWNDRNFFPAHEAWESCWKRSKGTEEEELFKGLSQLGAGYVHVRRMNHHGAKVLLRR
                     GLSRVVRYLPERRGLDLRAIAGAAREHADEVERAQRAGEPVPQVIPPTI"
     gene            7103..8413
                     /locus_tag="VF097_06305"
     CDS             7103..8413
                     /locus_tag="VF097_06305"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF006696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEX6262443.1"
                     /translation="MASALLTDLYELNMVASYVKRGMTASATFSLFIRELPSGWGFFV
                     ATGLEACLDELEGLRFTDEDLAYLRESQGYDEATLRGLADLRFTGDVVAVPEGRVVYP
                     TEPLLEVTAPLPEAQLVETILLNRVTYQTAVASKAARCVLAAPGKDMVDFALRRVHGG
                     EAGMGIARATAIAGFTATSNVEAARKFGLTASGTMAHSYIEAFDREEEAFRAFAQDFP
                     DQVVLLVDTYDTVRGVQKAIEVVRELPEVHLRGIRLDSGDLGELAHRARAMLDEAGLT
                     EVRIFASGGLDERKVAELADAPIDAFGVGSKVGVSEDSPTLDSVYKLVEYDDRPVAKL
                     STAKATLPGRKQVFRAEDGDTLALRGESLPGEPVLLPAMKGGERLNRDTWREARDRCR
                     ADLAALAEDRRGLDAPPIEIPRSEALAALDEEIRSDIRRRELSQ"
     gene            8439..9296
                     /locus_tag="VF097_06310"
     CDS             8439..9296
                     /locus_tag="VF097_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007624225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX6262444.1"
                     /translation="MDHVVFEREPELDRPVLICAFTGWNDGGEAASTAAAYLRDRWSA
                     ERFARLDPEEFFDFQVTRPTVRLVDGVTRRIEWPENDFFAASLPHRDVALFLGIEPNV
                     RWRTYTEQIIGTARAIGAETILLLGAFLADVPHTTTPPVTGSAPDEETAERLGLAGSR
                     YEGPSGIVGVLHDAATRTGLETISFWAAVPHYLPAGPNPRAALALVDKVARFLDVEIA
                     TDTLERAGATWESQVNAIIEDNEELASYVERLERSAQESDDLGEVPSGEALAAELERF
                     LRDQRGDGS"
     gene            9293..10066
                     /locus_tag="VF097_06315"
     CDS             9293..10066
                     /locus_tag="VF097_06315"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX6262445.1"
                     /translation="MINRKQRFRHRPDPIMPHEGPLPAEGEIASLVGGKSGALRAAIF
                     GVNDGLVSNLSLIMGVAGAGVSPEVILLAGIAGLLAGAFSMAAGEYISMRVQRELFER
                     LIHLEAHELGVDPEGETEELAELLEKRGIPGELAPQVAQSLMEDPATALEVHAREELG
                     LDPEELGSPWGAAGSSFVTFALGALVPLAPYLFAEGLLAAWLAVGLSLGALFGVGALM
                     TLFTGRSAALSGGRMMLIGGAAALVTFFVGKLLDVTALG"
     gene            complement(10067..10702)
                     /gene="pgl"
                     /locus_tag="VF097_06320"
     CDS             complement(10067..10702)
                     /gene="pgl"
                     /locus_tag="VF097_06320"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX6262446.1"
                     /translation="MERRLLVVDDLVSAGLGVFLGLGPRTIALAGGATPQPVYAALAE
                     HAYPWEEVDAFMGDERCVPLDHPESNFGKAREALLSKVPARAHPMPGETCDAVAYERT
                     LRERFGDEVPSIDLVFLGIGEDGHTASLFPGDAALDERERWVVPVDRPDFRRLTLTLP
                     VLNAAAAVMFLIDGETKREALTGLLDGDPEVPAARVEAERVLVVADRAAAG"
     gene            complement(10728..12665)
                     /locus_tag="VF097_06325"
     CDS             complement(10728..12665)
                     /locus_tag="VF097_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6262447.1"
                     /translation="MPRGHFPMVLREEDRRPIERATVRRVVQTFRPYRRRVLLIALTI
                     LVTAGLGVVNPLLIKVIFDTALFTPEGVQLGALYFWVSLMVAVPIVAGVISIGQTYLS
                     NIVGQRVMQDLRNALYRHLQRMPLRFFTSTRTGEIQSRLSNDIGGVQSVVTDTASSLL
                     SNTVIVLSTVIAMAILSWQLTVLSLAMLPFFLWLTAKVGKARQQVAASTQRSLADLSA
                     LAEETLSVSGMLLSKAFGRQRYHQDRFSKENERLAGLQIRQTMIGRSFFATVQIFFSI
                     APALVYLVAGWVISGSPSPAAAGITAGTIVAFTTLQSRLFFPIGQMLQVSVEVQSAMA
                     LFDRIFEYLDMEPEIQDRPDAVQLTHDDVEGRVRFRDVWFRYETPPDGEPSQDGDGDR
                     PRRLWTLQDVDLEIEPGQLAALVGPSGAGKTTITYLVPRFYDVQRGAVEIDGRDVRGV
                     SLESLGETVGMVTQETYLFHASVRENLMYGRPDATEEEMIAAAKAAFIHDRVMQLPDG
                     YDTPVGERGYKMSGGEKQRLAIARVILKDPRILILDEATSSLDTTSERLVQQALRPLM
                     EGRTTIAIAHRLSTILGADVIFVLDEGRVVERGTHDELLERGGLYATLYERQFHGGSV
                     EAELSDGVLLPSGEFVPRESA"
     gene            complement(12688..13371)
                     /locus_tag="VF097_06330"
     CDS             complement(12688..13371)
                     /locus_tag="VF097_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX6262448.1"
                     /translation="MRELDALRGEIASCTRCPRLVEWRERAARERPKRYLGWVYWARP
                     LPGLGDPRARLLIVGLAPAAHGGNRTGRIFTGDRSGDWLFRSLYRAGFASQPESVSRD
                     DGLRLAGCYVAAVNRCAPPGNRPTPEERDTCLPYLVRELELLTEVRVIVALGSYAWDG
                     DLRALRALGVEIPRPKPRFGHGVEVQVGPYRLLGSYHPSQQNTFTGKLTEEMFDAVFE
                     RARSLTDSE"
     gene            13513..14622
                     /locus_tag="VF097_06335"
     CDS             13513..14622
                     /locus_tag="VF097_06335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6262449.1"
                     /translation="MTTETTTVRVLLVDDVSASRETLRRALAFDERIEVVGEAGSGPE
                     AVEKAEQLRPDLVLMDVRMPDGDGVEATRHIVTRFPQTRVVALTAHDDVDTVRDMLTA
                     GATGYALKGASVDDLLSAVHKAWRGEAHIDDRVLPSAVDELRRLLREERERRTEVERL
                     ARIRQEFVQVLSHELRTPLTVMSGALRYLIRRGLTGEEEPLIASALQRGIELERMIEG
                     LELIGESRHDDRASASPCGAIEAALVRVEGHPDAVECDGGVWPGVRDRHLARIAFELI
                     DNAMRHGRPPVEVRAWASDGLAHLVVSDGGDADLRPELFEAFSAGDMSATRSRGGLGL
                     GLFVAQRLCEADLGRLAIRRDRGRTIAEVVYEIPA"
     gene            complement(14626..15132)
                     /locus_tag="VF097_06340"
     CDS             complement(14626..15132)
                     /locus_tag="VF097_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007074429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX6262450.1"
                     /translation="MGDAHEFGRYFEDFEVGDVYKHWPGKTITEYDDHLFCMITMNHH
                     PLHTNAHYAEEATQFKRNVVVGNLVYSLALGMSVPDVSGKAIANLEVESLQHAAPMFH
                     GDTLYAETTVLDKKESRSKPDRGVVTVETRGYNQDGVEVLYFRRKVLIPKRGTVPEQP
                     ARPEPGAE"
     gene            complement(15143..16312)
                     /locus_tag="VF097_06345"
     CDS             complement(15143..16312)
                     /locus_tag="VF097_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010310000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6262451.1"
                     /translation="MIELSEDQEAIVKAVREFVEQEVFPIADEMEHRDEFPEKIVEQL
                     KEMGLFGLTIPEEHGGAGMDVMTYALVVAELSRGWMSISGILNTHFMAAYVLRKHGTD
                     EQKERWLPRMATGEVRAAYSMSEAGAGSDVQAIKTKAERDGDHYVVNGSKMWVTNGLR
                     AGLVVTLVKTDPEADPPAKGMSLLYIEKEPGATEFEGITVPPNIPKMGYKGVETTELV
                     FENHRVPVENLLGEEEGQGFYQVMDGIEVGRVNVAGRAVGLATRAFEEAIRYAQEREA
                     FGKPIAHHQAIQFKLAQMGTKLEAARLMLLSAAQKKASGERSDLEAGMAKLFCSEACK
                     EIVEDALRIHGGYGYSAEYTVERLYREAPFLLIGEGTSEIQQMIIGRQLLERHKA"
     gene            complement(16309..>16669)
                     /locus_tag="VF097_06350"
     CDS             complement(16309..>16669)
                     /locus_tag="VF097_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017567865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX6262452.1"
                     /translation="GLQAIDGPYLLIKDLDGFREMAMRSRALGYDGKWALHPGQIDVL
                     NEVFAPTQEEYDKATAMLEAYRHATEVEATGAVMFGTEMIDEASRKMAEQVAERGRAA
                     GLEVTKTWDDFKKEWEQ"
BASE COUNT         2408 a         6084 c         5764 g         2413 t
ORIGIN      
        1 ccaccgctcc cgaggaggcg tcatcgaagc tcgaccgatg ctgagcgccg acgatgcgct
       61 cgcgggccgt cttcaggcgc tcgtcgatcg actcgcgtct cggaagagga tcccccacgc
      121 catcctgggc ctggcgagcg gggatggacc gctgcgctgg atcggcgctt cgggacccgc
      181 ccgtccggat ggaaccccga tgcggccgga cacgccgtac atgctcgcga gcgtcacgaa
      241 gctctacatc gggaccacga tcctgcagct ttacgaacgg ggcgaggtcg acctcgatgc
      301 cccgatcacc gcgtacctgg ccgtggaccg ggtcgccggc ctgcatcggc tcggaggcgt
      361 ggaccacacc tccaggatca ccgtccggca cctcctcggc catacttctg ggctccccga
      421 ctacctggag gaccgaccga gcgacgggcg ccgcatctac gaggagatcg aggacggcgt
      481 ggaccggtcg tggaccttcg acgacgtcat cgagatcgca cgtgggatgc ggcctcgctt
      541 cgagccgcaa gacctctccg ccggtcgcca gaaggcccgc tactcggaca ccggtttcca
      601 gctgctcctg atgatcatcg aatcggtcgc cggccggccc ttctacgagg tattcgagga
      661 ggggctgttc cgcccgttcg gcctgcggca cacgtgggtc cccggacgat cggagccgct
      721 cgaccctccc cccgacgacc cggcggccgt gtgggccaag ggacgaccga tccacatccc
      781 caaggcgatg gccagcacca acgaccttat ggggaccgcc gatgacaccc tgcggttcat
      841 gggcgcgctc acacggggcg aggtgttcca ggatccggcg acctaccggc tgatgcacga
      901 gcggtggaac cggatcttct acccgatgcg gtacggcctc gccacgatgc gctaccgcgt
      961 ccccaagctc ttcggtcccg gccgccgggc cgtgaccctg gtggggcact ccggaacgac
     1021 gggatcgtgg ctgctccact gccccgagct cgacctcgtg ctcacgggga cggtggacga
     1081 ggccagtgcc cgcgccgtcc cgttccggtt ccttccgcgg gtcctcaaag cactgcaggg
     1141 ctagggcatc atcccggcga cgtctccgct cagcctgccc actccttccg gagtacgagt
     1201 acctcctctc cctttggacc ttcgaccctc ggtcggctac gattgaggcg gtcgcgctta
     1261 tggctccgct cgaggtaagc gctaccttgg gggttttatg ggattgcgcc ggaggcgtcg
     1321 gcaatgggct cgcgctccgc ccgaagggtg tcgtcgcatc catcctgcct cgacagcgaa
     1381 ggaaggtgcc gctatgagac gtgtcctcgc gctgaccttg ttcctcggga tcctcgtcgc
     1441 gtccgccccc gcctcggccg agaagcccgt ccgcgagttc ctcccggctg aggatgaagt
     1501 gatctccgat gtgtgtgact tcgacctcgg agtggtcgtg ctggagaaca acgagctcct
     1561 gaccacgttc ttcgatcaag aagggaacgt gaccaagcag ctcgtcacgg gatcgctcgt
     1621 ggtggagcta cgcaacgagg agaccggggt gtcgctcgtg gcgaacatcc cggggcccgg
     1681 cgtcatcacg ttccccggcg actccgagct ggtgacggcg ctcggcccgt ggctctggct
     1741 cttcttcccc ggagatctgg ggcccggaga gccgggcatg gcgctcctca cccacggacg
     1801 attcgtggtc gagttcgcgc ccgacggggt cacgatcgtg gaacggcgcg gacgccaggt
     1861 tgacgtctgc gcgctcctct cctgacgagc ggtctcccgg gatgccatcg aggtccacgg
     1921 ggacctcgca cctccgtggg gatcggccgc cggccgcgag cggccggcgg ccgatccgat
     1981 gaagggaacg cccccggcgc gcgggcgcgc cgggggcgtt ccgccccgtc gggcgagagg
     2041 gcgctcctta cgggcctccc ggggtgccac cgggaccggc gcccgggggc agacctgggt
     2101 cggaggtcgg accggagccg ccgcctcctc gtccgttcga agcgccccgc ccgggggcat
     2161 ctccacgctc ggccttgccc gcagccgagg cctcggcccc cttggccttt ccggcctcgg
     2221 cggccggtcg gcctttcgcc ttccccgcgg ccgacatctc ctcggccttt actttgccgg
     2281 ccgcggacgc tccctcgccg ctcgatccgt tccgctcgca cggatcgtgg tcgtcgccgt
     2341 cttcctcctc ggcccgggcc gccgcggaga cgacctgccc gaactcgcac ccgaacccga
     2401 gctcgccgtc cacccaggcc tggatcatgg tcaggacgtc gatcgccgtc cggctggcgt
     2461 gctccgtcgc cccgctcccc tccccgaggg gcgcggggtc gaacccgtcg tcctcctcac
     2521 cggtcaggct ttccggctcc tccggctcga cgctctcaga gagctcctcg ggggaggtct
     2581 cgggcgatcc cggctgggcc ccggcgtagg cgagggctcc cagcgcggtc agggtggcga
     2641 ccgccacgat cgccaccctc acgggcatcg acctgaacgc gctgcgcaaa cgagatcagc
     2701 ctcctggctc gcctccatcc gcgtaatcga cggagagcgc cgagaggaaa cgggttgatc
     2761 cgagccttga cggaggggcg ctcgactgct ttgctcgccc tacgtcggcc caggagaaga
     2821 tcccatgcga cggatcgcgc tgctccccgc cctcacgctg ctgatctcgc tcacgcttcc
     2881 cgcggctcca tccgcgggga ccacggtcat ccacgtcacg acgaccgacg acgagatcgc
     2941 gaacgacggg gattgctccc tccgcgaggc gctgagcgcc gcggccacgg atgcgccgcg
     3001 cgacgcgtgc cccgcaggca gcggagggga cgtcgtgcgc ctgccggccg gcgtcttcgt
     3061 cctcaccatc ccgcccgacg ggacgcccga cgacggcgag gacggcgacc tcgacatctt
     3121 cgaccaggtg acgatccgcg gcgcaggggc gcgacggacg atcctcgacg ggaacgggac
     3181 cgatcgcgtc ctgcacgtgg gcccgatgtc cgtggtcagc gtgatggacc tgacgatcag
     3241 ggacgggtgg accgatgacg ccttcggcgg aggcgcgatc ctcaacatcg gggcgacgct
     3301 cacgctccgg cgtgtcaccg tgaagggcag cgaggcgacc gccggcgacg ggggtgggat
     3361 cagcaccagc aacgccggcg ccgtcctcac cgtggtcgac ggcagcgtga tcgacaacca
     3421 caccaccgcc ttcgggggca acggcggagg catcgccaac gtgaacgcct cggtggccaa
     3481 cctcgtccgc gtgaccgtga gcggtaactc ggcctttcag cagggagggg ggatcgccaa
     3541 cgaagccgga agcacgatcg atctcacctc ggtgacgatc acggacaacg tcgccaacgc
     3601 cgacgagggt ggagcggaag gcacgggggg cggcatccag cacagcggcg tggcggcgac
     3661 gctgcggcgc accctcgtcg cggggaaccg agacctcgat cccaacctcg gcgcccacga
     3721 ccccgactgc tcgagcatcg gctcgttcac gtcggagggc cacaacctgg tcggcgagcc
     3781 ctccgccggg tgcaacttcc ccgccgcggg cagcgaccgg atgggcgagc accccatgct
     3841 ccggccgctg cggaacaacg gcggccccac cgacacgcac gccctccgac gggacagccc
     3901 ggccgtcgac gcgatcccgg gcgcggcctg cgacccaagg gatcagcggg gggctccgcg
     3961 aagcggtcga tgcgacatcg gggcttacga gctcgtgcgc tgccgcggcg cgatcgtgaa
     4021 ccgcgtcggc acgggcggga aggatcggct caggggaacc gcgaagcgcg acggcttcct
     4081 cgggctggga gggggcgaca cgatccgcgg ccggaagggg aacgacggga tctgcggcgg
     4141 tccggggaag gacgtcctga tcggcggagc gggcagagac gtcctcgtgg gcgagaaggg
     4201 gcccgacacc ctccgggggg gcggcggccg ggacctgctg ctggggggcc cggggagcga
     4261 cgcgcttcgg ggcggcgcgg gcagggaccg ttgccgccag gggaagggca gcggaccggt
     4321 cagcggctgc gagcggtaag cgcctccgcc gcctcgtcca cgtcgcacac gcggatcagg
     4381 tcgtggtggc cggactcggt gacgatcagc tcccgtcgga aggcctcgac gagcgttccc
     4441 caccgctccc cgacgagcag cagcggccgg ggctcgaccg acatctgcat caggttccag
     4501 cagagggcga cctcggccag cgtgcccacc ccgcccgcga acgccaccca gacgtccgcg
     4561 tcgagcagca gcgggaaccg ggcgaggagg tcgcgggccg agacgacgtg ggtgagccag
     4621 gggttcggcg tgacgcggcc gacccaggcg gcgatcgaga tgccgatcgc tgcgccgccg
     4681 gcgccggcgg cccccctgct gaccgcctcc atgatgccgc cgtagccgcc gcagacgagg
     4741 tggatgccgg cgccggcggc gcgcgtcccc agctccaccg cgtccgcgta ctccggctcg
     4801 ccctcctcga tccaggcccc gccgaacacc gcagcccgcc gcggcgccgg gccaccctgg
     4861 aggagctccg aggagaaggt ggccggctcg gacacctcca cgctgccgtc cggccttccc
     4921 accgcgaggc ggtcgccctg ccgcgacacg gcggggcggc gggtgacgac ggcccaggcc
     4981 gccgcggcct cggccagcgg gtcgtcgccc tccggctcgg cgacgagggc cagccccagg
     5041 tcctccacga gggccgcggg gatcccggcg gaccggagcg tcaggcggtc catcgccgcc
     5101 gacctcggat cgggcgtggc gatcacgacc ccgtgggccc cggggccgag gacggtcatc
     5161 ggcggagccg gtgcgctccc ccggccaggc agtaggcccg gtagcccttc ccccacagct
     5221 gctcggccac gagctcggac cgcgatccca cggagcacac gaagaggtag ggctccttcg
     5281 gatcgagctg gggcagccac tcgtggaggc ggctgaacgg caggcgccgg tcgccgacga
     5341 ccggcccctc gccctcctct cggacgtcga tcacgatcac accgtccggc gcctgctcca
     5401 gcacgtactc gggcagcggc cccggcgtcc aggtcgcgag ctccacgacc cggcgcttct
     5461 ccaccgccgc gcggagcacc gactcatcga tctcgccatc ggcgtcggcc acctcctcga
     5521 accgagcggc ggtctccacc ggccccgtgg cgatcgagca gtactcgtgg acgcgtgcgg
     5581 aggcctcgtg ggtcccgatc tcgcgggccc ggcccatgat ctcctccttg ctcatcccca
     5641 ccagggggcg cagcacggga taggtcgtgg ctcgctccac gaccgagagg ttgcggagcg
     5701 tctgggtcga ggcctgcccg atcgactccc ccgtgacgac ggcgtctacc tgccgctcgt
     5761 cggcaaggat cgtcgccgcc cggtacatcg ctcgcttcag gacgagctgg cggagcctcc
     5821 cctcgacccg ctgggtcagg gcggccacaa ccgggagcag ctccacgatg tgcgctaccg
     5881 gctccagccc ccgcgcccat cggtccacga gcgccttggc cacggtgagg gccccgtcgg
     5941 tctgggcgca cccgccgagg tcgaacacca cgagctccac gctcgtcccg cggctcatcg
     6001 tcatccaggt ggcgacgggg gagtcgaagc cgccggagaa catcgccagg gcccggcccc
     6061 ccgtccccag cgggaggccc ccggcgccct tcgtcgcctc gagcaccaga tgggcgtcgt
     6121 cgttcacgat ctccacgggg acctcgacct cgggctcgtc caggttcacg cccgccgagt
     6181 cctcgagcag ggcggccccg agcttcaccg ccacgtcgtg ggaccggaag tcgtgctccc
     6241 ccgagcggcg gggacggatg gcgaaggtcc tcccccggat ccggtcccgc gaccgctcgg
     6301 cggccttcgc cacgaggtcg tcgaggccgt cgaacgtgac cgtctcgacc agcgagaccg
     6361 agtggaggcc gaagacgcga gacaggacct cggtggccgc ctccgggtcg gaggtgcgga
     6421 ccatgatccg gccccagccg gcctgcacgc tggcctgcac gccggcgcgg tcgagcgatc
     6481 gccgcacgtt ccgccgcagc acgcggagga accgacgccg cgtcctgggc gacttcacgc
     6541 cgatctcccc ggccgtcttc agcatcaggc gagccatcgg ctccatggta ggtcagatgg
     6601 tcggcgggat gacctgcgga acgggctcgc cggcccgctg ggcccgctcc acctcgtcgg
     6661 cgtgctcgcg cgcggcgcct gcgatcgccc ggaggtccag cccccgccgc tcgggaaggt
     6721 agcgcacgac ccgcgagagg ccgcgccgaa gcagcacctt ggccccgtgg tggttcattc
     6781 gccggacgtg cacgtagccc gccccgagct gcgacagccc cttgaagagc tcctcctcct
     6841 cggttccctt ggaccgcttc cagcacgact cccaggcctc gtgggcgggg aagaagttgc
     6901 ggtcgttcca gtaggcgatc gccagacggt ggttctcctc caggggcagc tggtcgaagt
     6961 cctccatcgg gagccggttc ggggcgtccc acgggagagg tcggcccagc tcgtcgcggg
     7021 gcctcttcgg cttggagggg gtggccatcg gagcgaggat atccggccca gccgggtcgc
     7081 cgaactctct atgctccaag gcatggccag cgcgctcctc accgacctct acgagctcaa
     7141 catggtggcg agctacgtga agcgcggcat gaccgcctct gccacgttca gcctcttcat
     7201 ccgtgagctc ccgtcggggt ggggcttctt cgtcgccacc ggcctggagg cctgcctcga
     7261 cgagctggag ggcctccgct tcacggacga ggacctcgcg tacctccggg agagccaggg
     7321 ctacgacgag gccacgctcc ggggcctggc cgacctccgc ttcaccggcg acgtcgtggc
     7381 cgtccccgag gggcgcgtcg tgtacccgac cgagcccctc ctcgaggtga ccgctccgct
     7441 ccccgaggcc cagctcgtcg agacgatcct cctcaaccgc gtcacctacc agaccgcggt
     7501 cgcctcgaag gcggcccgct gcgtgctcgc cgcccccggg aaggacatgg tggatttcgc
     7561 gctccgtcgc gtccacggcg gggaggcggg catggggatc gcccgggcca ccgcgatcgc
     7621 cgggttcacg gccaccagca acgtcgaggc cgcccggaag ttcgggctga ccgcctccgg
     7681 aacgatggcc cactcctaca tcgaggcgtt cgaccgcgag gaggaggcct tccgggcgtt
     7741 cgcccaggac ttccccgacc aggtcgtgct cctcgtggat acctacgaca cggtccgcgg
     7801 ggtgcagaag gccatcgagg tggtgcgcga gctccccgag gtccacctcc gcgggatccg
     7861 cctcgacagc ggggacctcg gggagctcgc gcaccgcgcc cgggccatgc tggacgaggc
     7921 cggcctcacc gaggtccgca tcttcgccag cggaggcctg gacgagcgca aggtggccga
     7981 gctggcggac gcgccgatcg acgccttcgg agtggggagc aaggtgggcg tgtcggagga
     8041 ctcccccacc ctcgacagcg tctacaagct cgtcgagtac gacgaccgcc cggtggccaa
     8101 gttgtcgacc gccaaggcca cgctcccagg ccgcaagcag gtcttccgcg ccgaggacgg
     8161 cgacacgctc gccctgcggg gcgagtcgct ccccggcgag cctgtgctgc tcccggcgat
     8221 gaagggcgga gagcggctga accgcgacac gtggcgagag gcgcgcgatc gctgccgggc
     8281 cgacctcgcc gcgctggccg aggaccgccg cgggctcgac gctccgccga tcgagatccc
     8341 caggtccgag gccctcgccg cgctcgacga ggagatccgg tccgacatcc gccgccggga
     8401 gctgagccaa tgagccgtcg acgggggaga ctgtgaccgt ggaccacgtc gtcttcgagc
     8461 gcgagccaga gctggaccgg cccgtcctga tctgcgcgtt caccgggtgg aacgacgggg
     8521 gcgaggcggc cagcacggcc gcggcgtacc tccgcgaccg gtggagcgcg gagcgattcg
     8581 cccgcctcga ccccgaggag ttcttcgact tccaggtgac ccggcccacc gtccggctcg
     8641 tggacggggt cacccgccgc atcgaatggc ccgagaacga cttcttcgcc gcctcgctcc
     8701 cccacagaga cgtggcgctg ttcctgggca tcgagccgaa cgtccgctgg cggacctaca
     8761 ccgagcagat catcgggacc gcccgggcga tcggggccga gacgatcctg ctcctgggcg
     8821 ccttcctggc cgacgtgccc cacacgacca ccccgcccgt gaccggctcg gcgcccgacg
     8881 aggagaccgc ggagcgcctc gggctggccg gctcccgcta cgaggggccc agcggcatcg
     8941 tcggcgtgct ccacgacgcc gccacgcgga ccggcctcga gacgatctcc ttctgggcgg
     9001 ccgtccctca ctacctcccg gccgggccca accccagagc cgccctcgcc ctggtggaca
     9061 aggtggcgcg attcctggac gtggagatcg cgaccgacac cctggagcga gcgggagcca
     9121 cgtgggagtc gcaggtgaac gcgatcatcg aggacaacga ggagctcgcc tcctacgtcg
     9181 agcggctgga gcgctcggcc caggagagcg acgatctcgg ggaggtaccc agcggcgagg
     9241 cgctggcggc cgagctcgag cggttcctgc gggaccagcg gggggacggc tcgtgatcaa
     9301 ccggaagcag cggttccggc accggcccga cccgatcatg ccgcacgagg gacccctgcc
     9361 ggccgagggc gagatcgcct cgctggtcgg cggcaagagc ggcgccctcc gggcggcgat
     9421 cttcggcgtc aacgacggcc tggtctccaa cctctcgctc atcatggggg tggccggcgc
     9481 gggcgtgagc ccggaggtga tcctgctcgc cggcatcgcg gggctgctcg ccggagcctt
     9541 ctcgatggcg gcgggagaat acatctcgat gcgggtccag cgagagctct tcgagcgact
     9601 gatccacctc gaggcccacg agctcggggt ggaccccgag ggagagaccg aggagctcgc
     9661 cgaactcctc gagaagcgcg gcatccccgg ggagctggcg ccccaggtcg cccagagcct
     9721 gatggaggac ccggccacgg cgctggaggt ccacgcccgc gaggagctgg ggctcgaccc
     9781 cgaggagctc ggctctccgt ggggcgccgc cggatcgtcc ttcgtgacgt tcgcgctggg
     9841 cgccctggtc ccgctggcgc cctacctctt cgccgaggga ctgctcgccg cctggctcgc
     9901 ggtgggcctc tcgctgggcg cgctgttcgg ggtgggcgcg ctgatgacgc tgttcacggg
     9961 acgcagcgcc gcgctctcgg gcggccggat gatgctgatc gggggcgccg ccgcgctcgt
    10021 gacgttcttc gtcgggaagc tcctcgacgt caccgccctg ggctgatcag ccggcggccg
    10081 cgcggtcggc caccacgagg acccgttcgg cctcgacccg agccgcaggg acctcagggt
    10141 cgccgtccag cagcccggtc agggcctccc gcttcgtctc gccatcgatc aggaacatca
    10201 cggcggcggc cgcgttcagc acggggagcg tcagcgtgag gcgccggaag tcggggcggt
    10261 ccacgggcac cacccaccgc tcccgctcgt cgagcgcggc gtcgccgggg aagagcgagg
    10321 cggtgtgccc gtcctcgccg atcccgagga agacgaggtc gatcgagggg acctcgtccc
    10381 cgaagcgctc gcggagcgtc cgctcgtagg ccacggcgtc gcaggtctcg ccgggcatgg
    10441 ggtgggctcg tgccgggacc ttcgaaagaa gcgcctcgcg cgcctttccg aagttcgact
    10501 cgggatgatc cagcggcacg catcgctcgt cgcccatgaa cgcgtcgacc tcctcccagg
    10561 gatacgcgtg ctcggccagc gccgcgtaca cgggctgagg ggtggcgccg ccggcaagcg
    10621 cgatcgtccg aggccccagc ccgaggaaca ccccgagccc ggcgctgacg aggtcgtcga
    10681 cgacgagtag cctccgctcc acgggcggaa gcctacccgg gaggggctca ggccgactcg
    10741 cgggggacga actcccccga ggggagcagg acgccgtcgg agagctcggc ctccaccgaa
    10801 cccccgtgga actgccgctc gtacagcgtg gcgtacaacc ctccgcgctc gagcagctcg
    10861 tcgtgcgtgc cgcgctcgac cacccgtccc tcgtcgagga cgaagatcac gtcggccccg
    10921 aggatcgtcg agaggcgatg ggcgatcgcg atcgtcgtcc ggccctccat cagggggcgc
    10981 agggcctgct gcacgagccg ctccgaggtc gtgtcgagcg acgaggtcgc ctcgtcgagg
    11041 atgaggatcc tcggatcctt caggatcacc cgggcgatcg ccagacgctg cttctcgccc
    11101 ccggacatct tgtagccgcg ctcccccacc ggggtgtcgt agccgtcggg cagctgcatc
    11161 acgcggtcgt ggatgaacgc ggccttggcg gcggcgatca tctcctcctc ggtggcgtcg
    11221 ggccgcccgt acatgaggtt ctcgcggacg ctggcgtgga agaggtaggt ctcctgcgtc
    11281 accatcccga ccgtctcgcc cagggactcc agcgagacgc ctcggacgtc gcggccgtcg
    11341 atctccaccg cgccccgctg gacgtcgtag aagcggggca ccaggtacgt gatcgtcgtc
    11401 ttgccggcgc cgctcggccc gacgagggcc gccagctggc ccggctcgat ctcgaggtcc
    11461 acgtcctgga gcgtccacag ccggcgcggt cgatcaccgt ccccgtcctg agaaggctcg
    11521 ccgtccggag gcgtctcgta ccggaaccac acgtcccgga accgcacccg cccctccacg
    11581 tcgtcgtgcg tcagctgcac cgcgtccggc cggtcctgga tctcgggctc catgtcgagg
    11641 tactcgaaga tccgatcgaa gagcgccatc gcgctctgga cctcgacgct gacctggagc
    11701 atctgaccga tcgggaagaa cagcctggac tgcagcgtgg tgaaggccac gatcgtcccc
    11761 gcggtgatcc ccgcggccgc cgggctgggg ctgccgctga tcacccagcc ggccacgagg
    11821 tacaccagcg ccggcgcgat cgagaagaag atctggaccg tagcgaagaa cgatcgcccg
    11881 atcatcgtct ggcggatctg gagcccggcc agcctctcgt tctccttcga gaagcggtcc
    11941 tggtggtacc gctgacgccc gaaggccttg gagaggagca tccccgagac cgagagggtc
    12001 tcctcggcca gcgccgagag atcggccagc gaccgctggg tcgaggcagc cacctgctgg
    12061 cgcgccttcc ccaccttcgc cgtgagccag aggaagaacg gcagcatcgc cagggagagc
    12121 acggtgagct gccaggagag gatcgccatc gcgatcaccg tggagaggac gatgaccgtg
    12181 ttcgacagga ggctcgaggc cgtgtccgtc accacgctct gcacgccgcc gatgtcgttc
    12241 gagagccgcg actggatctc gccggtgcgg gtcgacgtga agaaccggag aggcatccgc
    12301 tggaggtgcc ggtagagcgc gttccgaagg tcctgcatga cgcgctgtcc cacgatgttg
    12361 gagagatagg tctgcccgat cgagatcaca ccggccacga tggggactgc caccatgagc
    12421 gacacccaga agtacagcgc cccgagctgc acgccttcgg gcgtgaacag ggccgtgtcg
    12481 aagatgacct tgatcaggag cggattgacg acgcccagcc ccgccgtgac gaggatcgtg
    12541 agcgcgatga ggagcaccct ccgccggtag gggcggaagg tctggacgac ccggcggacg
    12601 gtggcccgct cgatcggacg cctgtcctcc tcccggagga ccatcgggaa gtgacctcga
    12661 ggcacggacc aaggatagcg gcgagtgtca ctcggagtcg gtcagcgacc tcgcccgttc
    12721 gaagacggcg tcgaacatct cctcggtcag cttcccggtg aacgtgttct gctggctcgg
    12781 atggtacgag ccgaggaggc gataggggcc cacctgcacc tcgacgccgt gcccgaacct
    12841 cggcttgggc cgcgggatct ccacccccaa cgcccgcagc gcccgcaggt cgccgtccca
    12901 cgcgtacgac cccagcgcga cgatcacgcg gacctccgtg agcagctcca gctcgcgcac
    12961 gaggtacggg aggcacgtgt cgcgctcctc cggggtgggg cggttcccgg gcggggcgca
    13021 ccggttcacc gccgccacgt agcacccggc gagccgcagc ccgtcgtcgc gcgacaccga
    13081 ctcgggctgg ctggcgaacc ccgctcgata cagcgagcgg aacagccagt cccccgaccg
    13141 atcccccgtg aagatccggc ccgtccggtt cccgccgtgg gccgcgggcg cgagccccac
    13201 gatcagcagc cgggcccgcg ggtccccgag cccgggcagc ggccgggccc agtacaccca
    13261 gcccaggtag cgcttcgggc gctcccgggc ggcccgctcg cgccactcga cgagcctcgg
    13321 gcagcgcgtg cacgaggcga tctcgccgcg gagcgcgtcc agctcacgca tgaggggtcc
    13381 gattgaagca ggtgtggtgg gccgcttggg ggaggacgga atcaccccat cgggcggtcg
    13441 aaatgccgag gccgtcccgc catgatggct caagcgctcc cgcaccgagc cgatgccaga
    13501 ggtacctact cgatgaccac agagaccacg accgtaaggg tcctcctcgt cgacgacgtc
    13561 tccgccagcc gggagacgct gcgacgtgcg ctggccttcg acgagcggat cgaggtcgtg
    13621 ggcgaggccg gctccgggcc cgaggccgtg gagaaagccg agcagctccg tcccgatctc
    13681 gtcctgatgg acgttcggat gcccgacggt gacggggtcg aggccacgcg tcacatcgtg
    13741 acgcggttcc cgcagacccg cgtcgtggcg ctcaccgccc acgacgacgt ggacacggtg
    13801 cgcgacatgc tcacggccgg cgcgaccggc tacgcgctga agggcgcctc ggtggatgat
    13861 ctcctcagcg cggtgcacaa ggcgtggcgt ggcgaggcgc acatcgacga tcgggtgctt
    13921 cccagcgccg tcgacgagct gcgtcgcctg ctgcgcgagg agcgagagcg ccgcaccgag
    13981 gtcgagcggc tggcgcgcat ccgtcaggag ttcgtccagg tcctctccca cgagctgcgc
    14041 acgccgctga cggtgatgtc cggcgcgctc cgctacctga tccgccgcgg gctcacgggc
    14101 gaggaggagc cgctgatcgc ctcggccctc caacggggca tcgagctcga gcggatgatc
    14161 gagggcctgg agctgatcgg cgagagccgg cacgacgacc gcgcctcggc cagcccgtgc
    14221 ggcgcgatcg aggccgccct ggtgcgggtc gagggccacc ccgacgccgt cgaatgcgac
    14281 gggggggtct ggccgggggt ccgcgaccgg cacttggcgc ggatcgcctt cgagctgatc
    14341 gacaacgcga tgcggcacgg ccggccaccg gtcgaggtcc gagcctgggc cagcgacggg
    14401 ctggctcacc tggtcgtctc cgacggcggg gacgccgacc tcaggcccga gctgttcgag
    14461 gcgttctcgg ccggcgacat gtcggccacg aggagtcggg gcggcctcgg gctcggcctg
    14521 ttcgtggctc agcggctatg cgaggccgat ttggggaggc tcgcgatccg ccgcgaccgc
    14581 ggccgcacga tcgccgaagt cgtctacgag atcccggcct gacggctact cggcccccgg
    14641 ctcgggcctc gccggctgct cgggcacggt ccctcgcttc gggatcagca ccttccgccg
    14701 gaagtacagg acctcgaccc cgtcctggtt gtagccgcgc gtctccacgg tgacgacgcc
    14761 gcgatcgggc ttcgacctcg actccttctt gtccaggacg gtggtctcgg cgtagagggt
    14821 gtcgccgtgg aacatcggcg ccgcgtgctg cagcgactcg acctcgaggt tggcgatggc
    14881 cttgccgctg acgtcgggga cactcatccc gagcgccagt gaatacacca ggttgcccac
    14941 caccacgttg cgcttgaact gcgtggcctc ctcggcgtag tgcgcgttcg tgtggagggg
    15001 atggtggttc atggtgatca tgcagaagag gtggtcgtcg tactcggtga tcgtcttgcc
    15061 cggccagtgc ttgtagacgt cgccgacctc gaagtcctcg aagtatcgtc cgaactcgtg
    15121 cgcgtcaccc atcgcgtccc tcctaggcct tgtggcgctc caggagctgg cggccgatga
    15181 tcatctgctg gatctcgctc gtgccctcgc cgatcagcag gaacggggcc tcccggtaga
    15241 gccgttccac cgtgtactcc gccgagtagc cgtacccccc gtggatccgc agggcgtcct
    15301 ccacgatctc cttgcacgcc tcggagcaga acagcttggc catccccgcc tcgaggtcgc
    15361 tccgctcccc cgatgccttc ttctgcgccg ccgagagcag catcagcctc gccgcctcga
    15421 gcttcgtgcc catctgggcg agcttgaact ggatcgcctg gtggtgcgcg atcggcttcc
    15481 cgaacgcctc tcgctcctgg gcgtacctga tcgcctcctc gaaggcccgc gtggccagcc
    15541 ccaccgcgcg gcccgcgacg ttcacccgcc ccacctcgat gccgtccatc acctggtaga
    15601 agccctggcc ctcctcctcg ccgaggaggt tctcgaccgg cacgcggtgg ttctcgaaga
    15661 cgagctcggt ggtctccacg cccttgtagc ccatcttcgg gatgttgggg gggacggtga
    15721 tcccctcgaa ctcggtcgct cccggctcct tctcgatgta gagcagcgac atccccttgg
    15781 cgggcgggtc ggcctcgggg tccgtcttca cgagcgtcac cacgagcccc gcgcgcagcc
    15841 cgttcgtcac ccacatcttc gagccgttga cgacgtagtg gtcgccgtcg cgctcggctt
    15901 tcgtcttgat cgcctggacg tcggagccgg cgccggcctc ggacatcgag tacgcggccc
    15961 gcacctcgcc ggtggccatc ctgggaagcc agcgctcctt ctgctcgtcg gtgccgtgct
    16021 tgcgcagcac gtaggccgcc atgaagtggg tgttgaggat cccggagatg ctcatccacc
    16081 cccgggagag ctccgccacg accaatgcgt aggtcatcac gtccatcccc gcgccgccgt
    16141 gctcctcggg gatcgtcagg ccgaagagcc ccatctcctt gagctgctcg acgatcttct
    16201 cggggaactc gtccctgtgc tccatctcgt cggcgatcgg gaagacctcc tgctccacga
    16261 actcccggac cgccttgacg atcgcttcct ggtcctcgct gagctcgatc actgctccca
    16321 ctccttcttg aagtcgtccc aggtcttcgt gacctcgagg ccggcggcgc gcccgcgctc
    16381 ggccacctgc tcggccatct tgcggctggc ctcgtcgatc atctcggtcc cgaacatcac
    16441 cgcgcccgtg gcctcgacct cggtggcgtg gcggtaggcc tcgagcatgg ccgttgcctt
    16501 gtcgtactcc tcctgcgtcg gggcgaacac ctcgttcagg acgtcgatct gcccagggtg
    16561 cagggcccac ttgccgtcgt agcccagggc ccgcgagcgc atcgccatct cccggaagcc
    16621 gtcgagatcc ttgatcagca gataggggcc gtcgatcgcc tggaggccg
//
LOCUS       DASUTZ010000144         4757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_819154, whole genome shotgun sequence.
ACCESSION   DASUTZ010000144 DASUTZ010000000
VERSION     DASUTZ010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819154"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VF097_06355"
     CDS             complement(<1..858)
                     /locus_tag="VF097_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX6262453.1"
                     /translation="MPSARARAGPRPAPERGTLRPVSDRRVTDSGIEVRPLYGPEEGL
                     EPERDLGRPGEPPFTRGIYPSMYRGRLWTMRQYAGMGTAEETNRRFRYLLDHGQTGLS
                     IAFDLPTQMGLDSDHPRAEDEVGRTGVAIDSVEDMHRLLEGIPLDRVTTSMTINATAP
                     ILLLLYELVAEEQGVPSEKLGGTVQNDILKEYAARGTYIYPPKPSMRLVTDLFAYCAD
                     RIPRWNTISISGYHMREAGSTAVQEVAFTLANGISYVRAALDAGLDVDDFAPRVSFFF
                     ACHMSFFEEV"
     gene            complement(815..2188)
                     /locus_tag="VF097_06360"
     CDS             complement(815..2188)
                     /locus_tag="VF097_06360"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX6262454.1"
                     /translation="MPLIRRAAALAAGLALVASSPGPGPAATAAAQDSPPNILIILSD
                     DQRAGTLGVMPKTRRWFKRAGVLYPRAYATTPVCCPSRASILTGRYAHNHDVNTNQEA
                     QELDHDTSIQRYLQDAGYRTGFLGKYMNAWPLLEPPPHFDRWALMAPARHRDATFNID
                     GTVRTVRRYSTDFIAERAARMIEELEATGDPWFILVAPYAPHGPFQPSKKYRRAKVPR
                     FRPTPSMLEKDRRDKPPYVQVRRQPLRNAKRIRRLQLRMLMSVDDLVGETMRALGRTG
                     ARQRTLAFYLSDHGYLWTDHGLKGKNHPYTESIRLPLLARWPGRLPPGTEDLRIATNV
                     DVPATILDAAGVAAPDPPLDGRSLLAPGVRDRLFTEAWPENHVPHWRSLRTDAFHYVE
                     YRDQETGDTTFREYYDLANDPWQLRNLLGDSNLANDPPPWMLTALSLQLERDAVCEGT
                     SGPSACP"
     gene            complement(2179..3546)
                     /locus_tag="VF097_06365"
     CDS             complement(2179..3546)
                     /locus_tag="VF097_06365"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX6262455.1"
                     /translation="MRRRIAAVTAAAIMGTLAAAPGGTSSEEEAVQQPNVLIVITDDQ
                     RAGTLKVMPRTRRWMKRGGTTFPSAFATTPLCCPSRASIYTGRYAHNHGVRTNDDPTR
                     LDQGTTIQRLAQDAGYRTAFFGKFFNRWTTNPLHFDHWETFHGVARYKRPEMNVNGTV
                     REFSRYSTDLLGARASRRISAWSGRPWLMYVAPIAPHAPYTPKHRDARAPVGRWPGNP
                     AVFERDRSDKPEWVRARSSTFRRGARIRRRQLRTLLAVDRMVDRLMRTLGQTGQRRDT
                     LVVFLSDHGLLWGEHGLGQKQSPYTASVRIPLMVRWPGHVARGAVDGRLAGTVDVVPT
                     ILEAAGIGPAPGAPPDGRSLLDPGWERDHVLLEFQKRQGKGVPSWASLRGTQEQYAEY
                     YDDAEAVSFREYYDLANDPWQLRNLLGDGSTGNDPPAGHLEALLTALARARVCEGTVG
                     ATACP"
     gene            complement(3539..>4757)
                     /locus_tag="VF097_06370"
     CDS             complement(3539..>4757)
                     /locus_tag="VF097_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX6262456.1"
                     /translation="DGDDVICGGPGDDGLLGGPGDPGADVLVGGPGNDGLFGVAGADE
                     QYGGPGDDLMTTGGGDPSDDKAAGGPGDDVVLNAGPGADLLLGGPGRDVLQAFAGPDE
                     VRGGGGPDVLVGGVGSDLLIGGSGHDRLSGGRGADRLHGRRGHDHLDGGSQRDRARGG
                     PGRDVCRSALSANGCSRPRRAVTSSGGRCAGLRATIVGTVGDDLILGTPGDDIVLGRG
                     GDDEIVGGKGNDVICGGDGHDGIRGGGGDDLLLGGPGDDMLLGGAGADEQRGGGGDDL
                     LDGGARDDGADLLIGGAEHDVLLGYAGDDVMRGGPGDDAFHAGRGDDQILGGPGRDRA
                     YGDDHDDLVVGGAGDDHVAGGFHDDRVFGGPGDDIVNGNDGFDELDGGDGIDVCLDAE
                     AGGAGCESDVRRA"
BASE COUNT          610 a         1761 c         1686 g          700 t
ORIGIN      
        1 cacctcctcg aagaagctca tgtggcacgc gaagaagaag gagacgcggg gggcgaagtc
       61 gtcgacgtcc agcccggcgt cgagcgcggc ccggacgtag gagatcccgt tggccagcgt
      121 gaaggccacc tcctggacgg ccgtggagcc cgcctcgcgc atgtggtagc ccgagataga
      181 gatcgtgttc caccgcggga tgcggtcggc gcagtaggcg aacaggtcgg tgacgagccg
      241 catcgagggc ttcggcgggt agatgtaggt cccgcgggcc gcgtactcct tgaggatgtc
      301 gttctgcacc gtgcccccga gcttctccga cgggaccccc tgctcctcgg ccacgagctc
      361 gtagagcagc agcaggatcg gcgcggtggc gttgatcgtc atcgaggtcg tcacccggtc
      421 gagcgggatc ccctcgagga gccggtgcat gtcctcgacc gagtcgatgg ccaccccggt
      481 ccggcccacc tcgtcctcgg cccggggatg gtccgagtcg agccccatct gggtagggag
      541 gtcgaaggcg atcgagaggc ccgtctgccc gtggtcgagg aggtagcgga agcgccggtt
      601 cgtctcctcg gccgtcccca tccccgcgta ctggcgcatc gtccagaggc ggccccggta
      661 catcgagggg tagatgccgc gcgtgaacgg cggctcgccc ggccgtccga ggtcccgctc
      721 gggctcgagc ccctcttcgg gaccgtacag cggccgtacc tcgatgcccg agtcggtgac
      781 gcgccggtcg ctcacggggc gtagcgtacc gcgctcaggg gcaggccgag ggcccgctcg
      841 tgccctcgca gacggcatcc cgctcgagct gcagagacag cgccgtcagc atccacgggg
      901 gcgggtcgtt cgcgaggttc gagtcgccga gcaggttccg gagctgccag gggtcgttcg
      961 ccaggtcgta gtactcgcgg aaggtcgtgt cccccgtctc ctggtcgcgg tactccacgt
     1021 agtggaacgc gtcggtgcgg agcgatcgcc agtggggcac gtggttctcg ggccaggcct
     1081 cggtgaagag gcggtcccgg acccccggcg cgagcaggga gcgcccgtcg aggggcgggt
     1141 ccggggcggc gacgcccgcg gcgtcgagga tcgtggccgg cacgtcgacg ttcgtcgcga
     1201 tccgcaggtc ctcggtcccg gggggcaggc gaccgggcca ccgcgccagc agcgggaggc
     1261 ggatcgactc cgtgtacggg tggttcttcc ccttcagccc gtgatccgtc cagaggtatc
     1321 cgtggtcgga gaggtagaag gcgagggtgc gctgccgggc accggtccgg cccagggccc
     1381 gcatcgtctc acccacgagg tcgtccaccg acatcagcat gcgcagctgg aggcgccgga
     1441 tccgcttcgc gttgcggagg ggctgacgac gcacctgcac gtaggggggc ttgtcgcgcc
     1501 ggtccttctc cagcatcgag ggagtgggcc ggaacctcgg gaccttcgcc cgccgatact
     1561 tcttggaggg ctggaagggg ccgtgcggcg cgtagggggc cacgaggatg aaccacgggt
     1621 ctcccgtggc ctcgagctcc tcgatcatgc gcgccgcccg ctccgcgatg aagtccgtcg
     1681 agtagcgccg gaccgtccgg acggttccgt cgatattgaa ggtggcatcg cggtggcggg
     1741 ccggcgccat cagcgcccaa cggtcgaagt ggggaggcgg ctccagcagg ggccaggcgt
     1801 tcatgtactt gcccaggaag ccggtgcggt agcccgcatc ctgcaggtag cgctggatcg
     1861 acgtgtcgtg gtcgagctcc tgcgcctcct ggttcgtgtt gacgtcgtgg ttgtgcgcgt
     1921 accggcccgt caggatcgaa gcccgggacg gacagcagac cggtgtcgtg gcgtaggccc
     1981 tcgggtagag gacgccggcc cgcttgaacc accgccgcgt cttgggcatc acgccgagcg
     2041 tcccggcgcg ctggtcgtcg ctgaggatga tgaggatgtt gggcgggctg tcctgggccg
     2101 cggccgtggc ggcgggcccg gggcccggcg aggacgcgac gagcgccagg cccgccgcca
     2161 gggccgcggc cctcctgatc aggggcacgc cgtcgccccc acggtcccct cgcagacccg
     2221 agcgcgcgcc agcgccgtga gcagcgcctc caggtgaccc gccggggggt cgttccccgt
     2281 cgacccgtcg ccgagcaggt tgcggagctg ccaggggtcg ttcgccaggt cgtagtactc
     2341 gcggaagctg acggcctccg cgtcgtcgta gtactccgcg tactgttcct gggtcccccg
     2401 cagcgaggcc caggagggga cgcccttccc ctgccgcttc tggaactcga gcaggacgtg
     2461 gtcccgctcc cacccggggt ccaggagcga gcgcccgtcg ggcggggcgc cgggggccgg
     2521 cccgatcccg gccgcctcca ggatcgtcgg gacgacgtcg accgtcccgg cgagccggcc
     2581 gtccaccgcg ccccgcgcca catggcccgg ccatcgcacc atcagtggga tccgcaccga
     2641 cgcggtgtac ggcgactgct tctgccccag cccgtgctcg ccccagagca ggccgtggtc
     2701 cgagaggaag acgaccagcg tgtcccgccg ctgacccgtc tggcccaggg tcctcatcag
     2761 ccggtccacc atgcgatcca cagccaggag ggtcctgagc tgacgcctgc ggattcgcgc
     2821 cccccgccgg aaggtcgagg accgggctcg gacccactcc ggcttgtccg agcggtccct
     2881 ctcgaagacg gccgggttgc ccggccaccg gcccacgggc gcacgcgcgt ccctgtgctt
     2941 cggcgtgtag ggcgcatgcg gcgcgatcgg cgcgacgtac atcagccacg gcctccccga
     3001 ccaggcgctg atgcgccggg acgcacgcgc gcccaagaga tcggtcgagt agcgggagaa
     3061 ttcgcgcacg gtgccgttca cgttcatctc ggggcgcttg tagcgggcca cgccgtggaa
     3121 cgtctcccag tgatcgaagt ggagggggtt cgtcgtccat cggttgaaga acttgccgaa
     3181 gaaggccgtg cggtagcccg cgtcctgggc cagcctctgg atcgtcgtcc cctggtcgag
     3241 ccgggtcggg tcgtcgttcg tccgcacgcc gtggttgtgc gcgtacctcc ccgtgtagat
     3301 cgaggcccgg gacgggcagc acagcggggt cgtggcgaac gcgctcggga acgtggtccc
     3361 tccccgcttc atccaacgcc gggtccgcgg catcaccttc agcgtgcccg cccgctgatc
     3421 gtcggtgatc acgatgagga cgttcggctg ctggacggcc tcctcctctg atgacgtgcc
     3481 gccgggcgcg gccgcgaggg tccccatgat cgccgccgcc gtcaccgccg cgatccgtct
     3541 acgcacgtcg cacgtcgctc tcgcatccgg ccccgccggc ctcggcgtcg aggcacacgt
     3601 cgataccgtc gccgccatcc agctcgtcga agccgtcgtt gccgttcacg atatcgtcgc
     3661 ccggtccccc gaagacccgg tcgtcgtgga agccgccggc gacgtggtcg tcgcccgcgc
     3721 cgcccaccac gaggtcgtcg tggtcgtcgc cgtaggcacg gtcgcgaccg ggcccgccaa
     3781 ggatctggtc gtcacccctc cccgcgtgga aggcgtcgtc tcccgggccc ccccgcatga
     3841 cgtcgtcccc cgcgtacccg agcagcacgt cgtgctccgc gccgccgatc agcaggtccg
     3901 cgccgtcgtc gcgcgcgccc ccgtcgagca ggtcgtctcc ccctccgccg cgctgctcat
     3961 cggcaccggc ccctccgagc agcatgtcgt ccccagggcc gcccaagagc aggtcgtcgc
     4021 cccctccgcc ccggatcccg tcgtggccgt caccgccgca tatgacgtcg ttccccttcc
     4081 cccccacgat ctcgtcgtcg cctccccggc cgaggacgat gtcgtccccc ggcgttccca
     4141 ggatcaggtc gtccccgacc gtccccacga tcgtggcccg gaggccggcg cacctgccgc
     4201 ccgaggaggt aacggcccgc cggggacggg agcagccatt agcagagagg gcggaacggc
     4261 agacgtcccg tcccggcccc cctcgggccc tgtcgcgctg cgaccccccg tcgaggtggt
     4321 cgtggcccct ccgtccgtgc aggcgatccg ccccccgtcc cccgctgagc cggtcgtgtc
     4381 ccgaccctcc gatcagcaga tcggagccga ctccccccac gaggacgtcg gggcccccgc
     4441 cgccccgcac ctcgtcgggg ccggcgaagg cctggaggac gtcccgtccg ggtccgccca
     4501 ggaggaggtc ggcgcccggc ccggcgttga ggacgacgtc gtctccgggg ccgcccgccg
     4561 ccttgtcgtc ggagggatcg ccgccccccg tcgtcatcag gtcgtcccca gggccgccgt
     4621 actgctcgtc cgccccggcc accccgaaga gcccgtcgtt ccccggcccc cccacgagca
     4681 cgtctgcccc ggggtccccg gggccgccga ggaggccgtc gtccccggga cccccgcaga
     4741 tgacgtcgtc accgtcg
//
LOCUS       DASUTZ010000145         3009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_170950, whole genome shotgun sequence.
ACCESSION   DASUTZ010000145 DASUTZ010000000
VERSION     DASUTZ010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170950"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            250..498
                     /locus_tag="VF097_06375"
     CDS             250..498
                     /locus_tag="VF097_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018224761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX6262457.1"
                     /translation="MILGRGAVHHYIPWQERARCREFDPEIFFPEKGGSSREAKRICA
                     QCEVRIECLNYALRHDERYGVWGGMSERERRRLKRMAS"
     gene            complement(479..826)
                     /locus_tag="VF097_06380"
     CDS             complement(479..826)
                     /locus_tag="VF097_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262458.1"
                     /translation="MLFRPDVFLRAAVFLRPVVFLRAADFLLAAVFLRPVVFLPAADF
                     LLAAVFLRPVVFLRAVVLRPVAFLRAAAVFFRAGAFLALDLFLPVAFFLAEDLFRAGG
                     IGQPPLPIRTPSA"
     gene            974..>3009
                     /locus_tag="VF097_06385"
     CDS             974..>3009
                     /locus_tag="VF097_06385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6262459.1"
                     /translation="MLAVLVVKDGADWLKRSLAALARQSYERLGVLAVDNASSDGSGK
                     LLRDLLGPRRVVSLRENVGFPGAVARAMEIPAAREADFLLLLHDDTVLHPDAVAGLVA
                     AAEGGVGVVGPKVLDGERRVLREIGYTIDRFGYRYSALEEGEIDQGQHDSPREVLFVS
                     GTAMLVARDAWLRAGPPDARLRPSHGDLDFCWRVRIAGFRVVMDPSAVVYHEAAGDAG
                     LREGARSDRTRYHEERSALTAMLKNYRLGTLLWVMPMYLVQGVAKVALFLLTRRFPDA
                     RQVLAAWAWTLWRFPGTVRRRVRLHAVRAVKEREVTATMAPTSARLRRWAGQVSAVLF
                     PARGASELAPEEEIALVPLRQRVGGFLGRYPAPVALGIGLLLTLVAFRGVLFASPLEG
                     GATPTFPDSARDVFAAYLDGTVPAGFGGADGASPALVPLGLLSVLTLGNPRTLAWIIV
                     ALTPLLAGVTAYRAALRRTGAPGAAVAAGASYALSAVVLWAASEGRVGEMAFLVGLPW
                     ILGRIADPFERPVGPPLRWALGTAVVLAITGSFFPAAWAPVAIATAVTLIVGGRRERR
                     LAGAGIALATLVMAGVLAFPFVLTLARVGVGRSAELGIAAEFGSILRLSPGEAPGSWL
                     PALFLPVGGVLGFALATQRAAAWRGLVSASLALPLAWLAAAGYLPSAAAEPIAYL"
BASE COUNT          386 a         1052 c         1149 g          422 t
ORIGIN      
        1 cggcggaact ctgatggagg agacgatctc gcggatggcc ggcgcggagt gggggatccg
       61 caaggagccc gaggagctcg agcaggcgat ccgggccatc ggccgggtgc ccgcccagcg
      121 caccacgacg tacggtcgcg ccgccgccca tctgtgagaa aggcggcggc ggcgcttcac
      181 acgcctccgg aaaaaagtca tcatggggcc tggacaagaa gagggcgtcc cggtcagaat
      241 tacacgcatg tgattctggg gaggggcgcc gtgcaccact acatcccatg gcaggagcga
      301 gcgaggtgcc gggaattcga cccggagatc ttcttccccg agaagggagg gtcgtcccgg
      361 gaggcgaagc gcatctgcgc ccagtgcgag gtgcggatcg agtgcctgaa ctacgccctg
      421 cgccacgacg agcggtacgg cgtctggggt gggatgagcg aacgggagcg gcgacgcctc
      481 aagcggatgg cgtcctgatc gggagagggg gttgaccgat gccaccggcg cggaagaggt
      541 cctccgccag gaagaaggcg accggtagga agaggtccag cgcgaggaag gcgcccgcgc
      601 ggaagaagac cgcggcggcc cgcaggaagg ccaccggccg caggaccacg gcccgcagga
      661 agaccacggg acgcaagaag acggcggcca ggaggaagtc ggcggccggc aggaagacca
      721 cgggacgcaa gaagacggcg gccaggagga agtcggcggc ccgcaggaag accacgggac
      781 gcaagaagac ggcggcccgc aggaaaacgt ccggacggaa gagcacgcgg cgccggtagg
      841 cgcgccgggc tcgggggagg ggccgtgagg ccccttctcc ctttccggat ggtgctatgc
      901 tccgccgcgg ctacgggcca cgcgccgccc tcggccgcat ccatccctcg tgggagttgc
      961 agacgcccct agcgtgcttg cggtcctcgt cgtcaaggac ggggccgact ggctgaagcg
     1021 gtcgctggcc gcgctcgccc ggcagtccta cgagcggctc ggtgtcctgg cggtggacaa
     1081 cgcgtcctcc gacggctcgg ggaagctgct gcgcgacctc ctgggccccc gccgcgtggt
     1141 gagcctccgc gagaacgtgg ggttcccggg ggccgtggct cgggccatgg agatccccgc
     1201 ggcccgggag gccgacttcc tgctgctcct ccacgacgac acggtgctcc accccgacgc
     1261 cgtcgctggc ctcgtggccg cggcggaggg cggcgtcggg gtggtgggcc ccaaggtcct
     1321 cgatggcgag cgacgcgtgc tccgcgagat cggctacacg atcgaccgct tcggctaccg
     1381 ctactcggcg ctcgaggagg gtgagatcga ccagggccag cacgactcgc cccgcgaggt
     1441 gctgttcgtc tccggcacgg ccatgctggt ggcccgcgac gcgtggctcc gggccggccc
     1501 acccgatgcg cgcctccgtc cctcgcatgg cgatctcgac ttctgctggc gggtccgcat
     1561 cgcagggttc cgggtcgtga tggacccctc ggccgtcgtt taccacgagg cggcggggga
     1621 cgccggtctc cgggagggcg caaggagcga tcgcacccgg taccacgagg agcggtcggc
     1681 cctgacggcc atgctcaaga actaccggct gggcacgctc ctgtgggtca tgccgatgta
     1741 cctggtgcag ggcgtggcca aggtggcgct gttcctcctg acgcgtcggt tccccgacgc
     1801 ccgtcaggtg ctcgccgcgt gggcgtggac gctgtggcgc ttcccgggga cggtgcggcg
     1861 acgggtccgg ctccacgcag tccgcgcggt gaaagagcga gaggtcaccg ccacgatggc
     1921 ccccacctcg gcccgcctgc ggcgctgggc aggacaggtc tcggcggtgc tcttccctgc
     1981 gaggggcgcg agcgagctgg cgcccgagga ggagatcgct ctcgtgcccc tgcggcagcg
     2041 ggtcggtggg ttcctggggc gctacccggc ccccgtggcg ctcgggatcg ggcttctcct
     2101 gaccctggtg gcgttccggg gggtcctgtt cgcctcgccc ctcgagggcg gtgcgacccc
     2161 gaccttcccc gacagcgccc gggacgtgtt cgccgcctat ctcgacggga ccgtccccgc
     2221 cggcttcggc ggcgccgacg gcgccagccc cgccctcgtc ccgctgggcc tcttgagcgt
     2281 cctgaccctc gggaaccccc ggaccctggc gtggatcatc gtggccctca ccccgctcct
     2341 ggcgggggtc acggcatacc gggccgccct gcgccggacg ggcgcgcccg gggcggccgt
     2401 ggcggcggga gcctcctacg ccctctcggc cgtcgtgctg tgggccgcct ccgagggccg
     2461 ggtgggagag atggccttcc tggtcggcct cccctggatc ctcggccgca tcgccgatcc
     2521 gttcgagcgg ccggtgggcc cgccgctgag gtgggcgttg ggcaccgccg tcgtcctggc
     2581 catcacgggg tcgttcttcc cggctgcctg ggcgcccgtg gcgatcgcga cggcggtgac
     2641 gctgatcgtc ggcggacggc gggagcgccg cctggcgggc gcggggatcg ccctcgccac
     2701 actcgtcatg gcgggcgtgc tcgccttccc cttcgtgctc accctggctc gggtgggggt
     2761 gggccgaagc gccgagctcg ggatcgccgc ggagttcggg tcgatcctga ggctctcgcc
     2821 cggggaggcc cctggctcgt ggctgcccgc tctcttcctc ccggtgggcg gggtcctcgg
     2881 cttcgccctc gccacgcagc gtgccgctgc gtggcgaggg ctcgtctcgg cgagtctcgc
     2941 cctcccgctc gcctggctgg cggcggccgg gtacctgccg agcgcggcgg ccgagcccat
     3001 cgcgtacct
//
LOCUS       DASUTZ010000146         4269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_820228, whole genome shotgun sequence.
ACCESSION   DASUTZ010000146 DASUTZ010000000
VERSION     DASUTZ010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820228"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VF097_06390"
     CDS             complement(<1..624)
                     /locus_tag="VF097_06390"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6262460.1"
                     /translation="MTHDLLTIGAGAAGEYAAGLVAEAAGRVAVVERSLVGGQCAFWA
                     CMPSKTLLDAAGRRALGDDYPWERASARRDWMISREEIDVPDDSSHARALEEQGAEVI
                     RGEARVTGPGTVEVRTEDGTRSLEGRSLLIAAGSVPVIPEIEGAEEAGYWTSNEATST
                     RDLPSSLVILGSGVVGVELAQAFARFGTKVTLVEIHDRILAKDHPRSS"
     gene            700..2430
                     /locus_tag="VF097_06395"
     CDS             700..2430
                     /locus_tag="VF097_06395"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6262461.1"
                     /translation="MAEEQAKFRGWKLWTMVAVVLGSGIVFLDTSVVNLALPKIADEL
                     PRGAFAKLEVQGYVQNGYFVTLSALLILAGALTDYHGRRKVFAIGLAGFGLTSLLCGL
                     APNAQLLIVFRILQGAAGAFVVPGSLSIIASSFDGEEQGRAFGLWAAASAATTIIGPF
                     IGGLLVDTISWRAAFLINVPLILIALYATLRHVPESRDEEATGRFDVMGSLIIIVAIA
                     GLSFGPVRGEQSQWRELLVPLVSIGVGLAAAIAFPFVMKRSRDPLVPLGLFRSRNFSV
                     TNISTFVIYGALYVVLGFMGIFLIGTLGYTARAAGLAMVPATLLLVLFSSRFGKLSAR
                     FGPRFFMSAGPAIMGVGLLWFLRFPDGSAPWALGTGPGETLLPPADYWVHIFPGILVF
                     GAGLMMMVAPLTTALMTSLPKRTSGLASAINNAISRVGSPIVFALIFVVIAAGFYGSI
                     QDRVPEAPVNESSFRRDVSPLNAPPDDASADLAAATREASTDAFRLAMLVASLMLFAG
                     AAVNAAGISNRQAIEYPHAEPGERLPEEEEAEPATPPDHELAPAPACVICGEEGTAAS
                     HGHAVDAPDT"
     gene            2427..3047
                     /locus_tag="VF097_06400"
     CDS             2427..3047
                     /locus_tag="VF097_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018485431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEX6262462.1"
                     /translation="MTERRPAPLLDLLDWKRRVFDLYEEVRSAPDPVVAWELWRATRD
                     RMMREHPQSPIPPGQRDAFGGLRYFEYDRSFRVLAEVEEAEPERLEVVTSRDGAYAFT
                     RFGRARFELAGENASLDLLWLEGYGGGVFVSFRDATSGRETYGAGRYLWDTVKGADLG
                     LEDGLMVLDFNFAYNPSCAYDPRWDCPLAPPGSRLEIPVTAGERAP"
     gene            complement(3099..4013)
                     /locus_tag="VF097_06405"
     CDS             complement(3099..4013)
                     /locus_tag="VF097_06405"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6262463.1"
                     /translation="MRKTFRTPVLGAALVALALIAAACNGSGEGGDGDGASRGQITVG
                     AVAFAENQILGEMYAQALEDAGYEVSRQLSLDSREILQPAMESGEVDVAPEYLATLSA
                     FLGGETSSDPDEQVEILEPLLEEQGQTVLDYSEAIDTNAFVVTRETAEQFNLSAVSDL
                     QGVAGDMTLGGPPECPEREFCIPGLRDVYGVEFGQFVPLDVGGPLTVEALSNGEIDVG
                     LLFSTDGAIPANDFVLLEDDMSLQQADNIAPVVRTEVLDEEIRTILDAISAALTTEKM
                     TALNAQVTVEGGDPAEAARTFLDEEGII"
     gene            complement(4083..>4269)
                     /locus_tag="VF097_06410"
     CDS             complement(4083..>4269)
                     /locus_tag="VF097_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020639272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6262464.1"
                     /translation="RYIIDGFAVRDLAQIFAGAVLVAALAILVDVLIGGVGRLLSPRV
                     ATRPGAATNHGITVGAP"
BASE COUNT          564 a         1535 c         1482 g          688 t
ORIGIN      
        1 cgaggaccgg gggtggtcct tcgccaggat ccggtcgtgg atctcgacca gggtcacctt
       61 cgtcccgaag cgggcgaagg cctgggccag ctccacgccc acgacgcccg agcccaggat
      121 gacgaggctg gacggaaggt ctcgcgtgga ggtggcctcg ttgctcgtcc agtagccggc
      181 ctcctcggcc ccctcgatct cagggatcac gggcaccgag cccgccgcga tcagcaggct
      241 ccgaccctcc agggaacgcg tcccgtcctc ggtgcggacc tcgacggtgc cggggcccgt
      301 gacgcgggcc tcgccccgga tcacctccgc cccctgctcc tccagcgccc gcgcgtgtga
      361 ggagtcgtcg gggacgtcga tctcctcccg gctgatcatc cagtcgcggc gggccgacgc
      421 gcgctcccac gggtagtcgt cgccgagcgc gcgtcggccc gccgcgtcca gcagcgtctt
      481 cgacggcatg caggcccaga aggcgcactg ccctccgacc agggagcgct cgacgaccgc
      541 gacccggccg gcggcctcgg ccaccaggcc ggccgcgtac tcgccggccg ccccggctcc
      601 gatcgtcagc agatcgtggg tcacggaata cctccccggt gagggggttc accgtactag
      661 agtcgcgtct ggcgccccga tattccccgg aaggagacca tggcggagga gcaggccaag
      721 ttccgcggct ggaagctctg gacgatggtg gcggtggtcc tcgggtcggg gatcgtcttc
      781 ctcgacacct cggtggtcaa cctggccctc cccaagatcg cggacgagct tccgcgtggc
      841 gcgttcgcaa agctcgaggt ccaggggtac gtgcagaacg ggtacttcgt gacgctctcc
      901 gccctgctga tcctggcggg cgcgctcacc gactaccacg gtcgccggaa ggtgttcgcg
      961 atcgggctgg ccgggttcgg gctgacctcg ctgctgtgcg gcctggcccc gaacgcgcag
     1021 ctgctgatcg tcttccggat cctgcaggga gcggccggcg cgttcgtggt ccccgggtcg
     1081 ctctcgatca tcgcgtcgag cttcgacggg gaggagcagg gacgggcctt cggactgtgg
     1141 gccgccgcct cggccgcgac cacgatcatc ggccccttca tcggcggcct gctcgtcgac
     1201 acgatctcct ggcgggcggc gttcctcatc aacgtgccgc tgatcctcat cgcgctctac
     1261 gcgacccttc gccacgtgcc cgagagccga gacgaggagg ccacggggcg cttcgacgtc
     1321 atgggctcgc tcatcatcat cgtcgccatc gccggcctgt cgttcggccc ggtccgcggg
     1381 gagcagtcac agtggcggga gctcctcgtg ccgctggtga gcatcggcgt cggccttgcg
     1441 gcggccatcg cgttcccctt cgtgatgaag cggtcgcggg atccgctcgt cccgctcggc
     1501 ctcttccgct cccgcaactt cagcgtcacg aacatctcga cgttcgtgat ctacggcgcc
     1561 ctctacgtgg tgctgggatt catgggcatc ttcctgatcg ggaccctggg gtatacagca
     1621 cgcgccgcgg gcctggccat ggtcccggct acccttctcc tggtcctgtt ctcgagccgg
     1681 ttcggcaagc tctcggcccg cttcgggccc cggttcttca tgtccgcggg accggcgatc
     1741 atgggagtcg gtctgctttg gttcctccgc ttcccggacg gctcggcccc gtgggctctg
     1801 gggacgggcc caggagagac gctgctgcct ccggcggatt actgggtgca catcttcccc
     1861 ggcatcctcg tcttcggagc gggcctgatg atgatggtcg caccgctcac cacggccctg
     1921 atgacctcgc tccccaagcg gacgtcgggg ctggcctcgg cgatcaacaa cgcgatctcg
     1981 agggtgggct cgccgatcgt gttcgcgctg atcttcgtgg tgatcgcggc gggcttctac
     2041 gggtcgatcc aggaccgggt cccggaggcg cccgtgaacg agtcgtcgtt ccgtcgagac
     2101 gtctcgccgc tgaacgcccc gcccgacgac gcgtccgcag acctggccgc ggccacgcgg
     2161 gaggcctcca cggacgcctt ccgcctggcg atgctggtgg cctcgctgat gctgttcgcg
     2221 ggcgcggcgg tgaacgcggc cgggatctcg aaccgtcagg cgatcgagta cccgcacgcc
     2281 gagcccgggg agcggctgcc cgaggaggag gaggccgagc ccgcaacccc gccggatcac
     2341 gagctggccc ccgcccccgc ctgcgtcatc tgcggggagg agggcacggc cgcgtcccac
     2401 gggcacgcgg tggacgcccc cgacacgtga cggagcgccg gcccgccccc ttgctcgacc
     2461 tcctcgactg gaagcgccgc gtcttcgatc tctacgagga ggtccggtcg gccccggacc
     2521 cagtggtggc ctgggagctc tggcgggcga cccgcgaccg gatgatgcgt gagcatcccc
     2581 agtcgcccat cccgccgggg cagcgcgacg ccttcggcgg cctccgctac ttcgagtacg
     2641 accggtcgtt ccgggtgctg gccgaggtcg aggaggcgga gccggagcgg ctcgaggtcg
     2701 tgacgagtcg ggacggcgct tacgcgttca cgagattcgg gagggcgagg ttcgagctcg
     2761 cgggtgagaa cgcatccctc gacctcctgt ggctggaggg ctatgggggc ggcgtgttcg
     2821 tctcgttccg cgacgcgacg agcgggcggg agacgtacgg ggccggccgc tacctctggg
     2881 acacggtgaa gggggccgat ctggggctcg aggacggcct gatggtcctg gacttcaact
     2941 tcgcctacaa cccgagctgc gcctacgacc cccgctggga ctgtccgctg gcgccccccg
     3001 gcagccggct cgagatcccg gtgaccgcgg gggagcgggc tccgtgaacg aggagggccc
     3061 gggcgcgcgc gcccgggccc tccgcgaggc atagggcctc agatgatccc ttcctcgtcc
     3121 aggaaggtcc gcgctgcctc cgccgggtcg cctccctcca ccgtgacctg cgcgttgagc
     3181 gcggtcatct tctcggtggt gagggcggcg ctgatcgcgt cgaggatggt gcggatctcc
     3241 tcgtccagga cctcggtgcg gacgacgggc gcgatgttgt ccgcctgctg gaggctcatg
     3301 tcgtcctcga gcagcacgaa gtcgttggcc gggatcgcgc cgtcggtgga gaacagcagg
     3361 ccgacgtcga tctcgccgtt cgagagcgcc tcgaccgtca gcggtccgcc cacgtccagg
     3421 gggacgaact ggccgaactc gaccccgtag acgtcccgca gccccgggat gcagaactcc
     3481 ctctcagggc actcgggtgg gccgcccagc gtcatgtcgc cggccacgcc ctgcaggtcg
     3541 ctcacggccg agaggttgaa ctgctcggcg gtctccctcg tcaccacgaa ggcgttggtg
     3601 tcgatcgcct cggagtagtc gaggaccgtc tgtccctgct cctcgagcag cggctccagg
     3661 atctcgacct gctcgtcggg gtcgctcgag gtctcgccgc ccaggaacgc cgacagcgtc
     3721 gccaggtact cgggcgccac gtcgacctcg ccgctctcca tcgcaggctg caggatctcg
     3781 cgcgagtcga ggctgagctg gcggctgacc tcgtagcccg cgtcctccag cgcctgcgcg
     3841 tacatctctc cgaggatctg gttctcggcg aaggcgacgg cgcccacggt gatctggccc
     3901 cgcgacgccc cgtcgccgtc cccgccctca cccgacccgt tgcacgccgc cgcgatcagc
     3961 gccagcgcga ccagggccgc cccaaggacg ggggtgcgga aggtcttccg catgggtacc
     4021 ccctctctct tcacggggag ccgaaggccc ccctggaaca tgggcgacag caatcttagt
     4081 aactacggag ctccaaccgt gattccgtgg ttcgtcgcgg cgccgggacg cgtggccacg
     4141 cgcggcgaga ggagccggcc gacccctccg atcagcacgt ccaccaggat cgccagcgcc
     4201 gcgacgagga ccgctcccgc gaagatctgg gcgaggtccc gaaccgcgaa gccgtcgatg
     4261 atgtagcgg
//
LOCUS       DASUTZ010000147        10574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1726812, whole genome shotgun sequence.
ACCESSION   DASUTZ010000147 DASUTZ010000000
VERSION     DASUTZ010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10574
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1726812"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..404)
                     /locus_tag="VF097_06415"
     CDS             complement(18..404)
                     /locus_tag="VF097_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX6262465.1"
                     /translation="MLKPGTSATVAETAAEEQTAERIGSGDVPVYATPMLLALVERAA
                     VAAIKTALDPGTTSVGARVELDHLAPTPPGSRIEATATLEQVDGRRLVFSLEARDPGG
                     VVARGTHVRVLVERGPFLEGARSRVV"
     gene            426..1214
                     /locus_tag="VF097_06420"
     CDS             426..1214
                     /locus_tag="VF097_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262466.1"
                     /translation="MEGASGVIALVLAETAAGSAAVLWLTGLWGNVKRGFFILTTATV
                     LACALLAALSANAGATEAGSGGARLVVVLALALSALSGLTLIALILRRMAAGRVLGLV
                     SIPVGAALLVAMASVAEAGMAMALVGLFAGAAFMGAVTVGLLLGHWYLVDRGLARDHI
                     QRMAVVLIGAVLVEAVAVIIAAVAGEVTPTEGFGLFLTVGGAAFHLAIVLGMVAITAL
                     IAFLIRAALRETRPRAVQAATGFFYLAVITAFTAELAAKVRFLD"
     gene            1216..1836
                     /locus_tag="VF097_06425"
     CDS             1216..1836
                     /locus_tag="VF097_06425"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX6262467.1"
                     /translation="MDAVAVAVAWIGPLAVAGAWAAVRVGGVSIWAAMGWTLGPLGAL
                     ALLTGGVEAGGGLLEALIGVGAGLALYAATATFLFLARGWRILRRQAHELYARRRGLS
                     LGAVLAIACLVAVPGEESLWRGLIQGGLGEALGEAPGAALAWALYVLVNLVSGSVPIV
                     LGAAVGGAAWTALALWSGGILASIACHVVWAGLMILRPPVGEGSDA"
     gene            1833..2771
                     /locus_tag="VF097_06430"
     CDS             1833..2771
                     /locus_tag="VF097_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262468.1"
                     /translation="MISEEARRVLRDGVLLYAAAPGPDAPHLTPMVFVLAEERLWATT
                     ARGSRKVRLWRAMPEGGGLVRVGDRAVSFRGRVDLYDLLDPTTWGRSLFRSPLLTRAA
                     LGFSRKNARFFAGYARDAHRVPFGWTPPGRVFLSIALRDGVLLDLSEGEVLRSWGTLD
                     EGLTGAASFRRAPPRPAPDAGVPGDVLDALGDEGRGALALGDAPVLPVRWRRSQEEVA
                     YYAVVPAPFLERTGAADRARATLVLDHLSRWRASRMLGMQLRGEGRVYVPGRLRSGGA
                     SLVDRSGATEGDAVVRLRPDRVVWWRGWASGSLGAR"
     gene            2768..3211
                     /locus_tag="VF097_06435"
     CDS             2768..3211
                     /locus_tag="VF097_06435"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6262469.1"
                     /translation="MSTIRDAVVVDATPDEVWRVIGDPRNLPRWNRQIRAVAGAPDGE
                     LREEDSYRVQLGFLGVSAEVRAVVKRLERPTYSEVALSGPIEGVVRTFVRPIGASRSR
                     VEHEVEYRFRGGALGRAAARAVRLMGASLILRRGLRAQKEMVEGR"
     gene            3208..3810
                     /locus_tag="VF097_06440"
     CDS             3208..3810
                     /locus_tag="VF097_06440"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX6262470.1"
                     /translation="MRRRRRRVGRALALAGAALGLAAITGSVRGRAFDERIYKQVNTN
                     LEHPTLDRLLSGVTELGSLYASLGAGAVIASSGSRRAAARAVGAAVSMWALGQALKSY
                     YRRARPFDSTAPGRLLIGKPRGTSWPSSHPAVFLTFALVAARRLDLPGPARSALVALA
                     GAVGLSRVYLGVHFPSDVAGGLLLGRAMADVWTAQDPDVR"
     gene            3821..4831
                     /locus_tag="VF097_06445"
     CDS             3821..4831
                     /locus_tag="VF097_06445"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEX6262471.1"
                     /translation="MDALATLSWPVLDRIELGPLAISPHGIGIAVGYLLGSWWMMRDG
                     PKRGLEEEHVTTIILWALVGAIVGARFFYVLGHLDEFESLTDMLAIWRGGISLVGGIF
                     GAIGFAVPLMRRYGYRFGQVMDSAAVGLAFGIAVGRIGDLVIGDHLGKPTDFLLGWRY
                     TSGTLPGPYQAAGPETWIASLAGPHCQLIRPEGAELYEGTCDLSGALLAQGPGVHQTA
                     LYDMLIAGALFAFLFWLSRKPRREGVLIAAFGIWYGAGRLLTDFLRVDKTWPLGLTGS
                     QWASVGVILAAVAVLVRFALRPRPAPVTIPEAETGRGELPSGPTTSFTPPEEPRLRPP
                     GG"
     gene            complement(4812..5906)
                     /locus_tag="VF097_06450"
     CDS             complement(4812..5906)
                     /locus_tag="VF097_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010047745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEX6262472.1"
                     /translation="MITEQDVRDALHRALDPSIEKPLDELGAVEAVEIERDRVRVSIR
                     VPIPGDDEAVRRSVEEAVAGLGAHLELRVSEMSPEQRAEMVGRLRGGKTREETVFFSE
                     GPTEVIAVASGKGGVGKSSVTANLAASLAAAGQRVGILDADVWGFSIPRMMGVGGQPA
                     GTSDMIIPPRAHGVKIISMGFFVSDETPVIWRGPMLHKAIQQFLGDVYWGELDALFVD
                     LPPGTGDVSISLASFLPRGAMLVVTTPQEAAAKVAERAGRMAQNPNIDLEVLGVVENM
                     AGFVCPHCGEITPLFGEGGGARTAETLGVPLLGSVPIQVSLREGSDEGTPIVVSEPDS
                     PAGAAFRAIAAELGTRIRSPRGKPLSLREA"
     gene            5978..6661
                     /locus_tag="VF097_06455"
     CDS             5978..6661
                     /locus_tag="VF097_06455"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6262473.1"
                     /translation="MDTKALEIHKALADNTRFRLYRHLRLMDRPVSVREMSRRLSLHP
                     NTLRPHLRRLEEAGLVRHELRKSDGVGRPQMLYVGVEPAGDEGRDFRLLSEMLLGTIQ
                     TRRQLERATALARGWGEYLVSRDAPKPGVRLPDRKNLAVLQSAMDRAGFEPRFRRQGT
                     SVEVTFRRCPFRELVDDHREVVCGLHRGLLEGMLGGLRPPLELRELKPFVERGVCRLR
                     AAPPRRKRG"
     gene            6706..7281
                     /locus_tag="VF097_06460"
     CDS             6706..7281
                     /locus_tag="VF097_06460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6262474.1"
                     /translation="MGSTDAPDGPLTPEAERGLVRRLISGDEEAVRILYGRFGGPVYG
                     LGLRLLGSPEAAQELTQDTFLAVWRKAPQYDPARGRLSTWIMVIAHNLGVDRLRREAR
                     RPVALVEDVETLGGEDVETEARILDRVATLELLSVLSAAERRLLGLAYYRGWTAREIS
                     LHDSIPLGTVKTRLRSALIKLRRAHGWKREL"
     gene            7281..8012
                     /locus_tag="VF097_06465"
     CDS             7281..8012
                     /locus_tag="VF097_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX6262475.1"
                     /translation="MDCAEVRGMLPEHALGTLGVSDLARVREHLAACAGCRKETGELM
                     EGVEVAALATAAPPPPELEDRVVAAVARAAGRRAGRRRLPVAGLLAAALLLAVGWGAA
                     MAGRAERLGDQAEIARRQAAQAAGSFGEFVGDLSGGGARETVLRSTSRVGAGGRAIVF
                     DSDVSGASDWVLVVVGGLPDEEGPYRAKLRGREGDLPVGRLWPSAEGRLAAYALFGAL
                     PGDGEDVVVLDADGAVVLKGRLEAR"
     gene            8046..9053
                     /locus_tag="VF097_06470"
     CDS             8046..9053
                     /locus_tag="VF097_06470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX6262476.1"
                     /translation="MRLGLALPHYDFSFADGAPVTWERLRSWGTAAEEMGFDSIWISD
                     HFFLSLARYGGRDEPFGTPEALTALAALATTTDRVRLGTLVLSAGFRHPAIVAKAAAV
                     IDLLSGGRLDLGIGAGWYEDEYRAFGYEYEDARGRFDVLEESLIVLGELLGDGPATWR
                     GERFSLDGAYNRPAPVQRPRPPIWVGGKGGPRLQRLVARHGDGWNSVWAWSPERYREK
                     VDELRATCEREGRDPASVRLSVGLYLAIGRDERGVRDVLERYAERVPGGVPASEDVDT
                     WRSERLAGTPPEVLERLGAFAELGVEEVIVSPASVPFAVHDEDALALFAQAVLPTARD
                     L"
     gene            9053..9274
                     /locus_tag="VF097_06475"
     CDS             9053..9274
                     /locus_tag="VF097_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEX6262477.1"
                     /translation="MDELEAAVRVLEEEGEPLHWTVIQDLALKRGYLDPFTRKDIRRR
                     LIAALADAAKRSDHPIRKAGKGVYTLGER"
     gene            complement(9253..9534)
                     /locus_tag="VF097_06480"
     CDS             complement(9253..9534)
                     /locus_tag="VF097_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEX6262478.1"
                     /translation="MGSFEEAVTVVVRALGPGEVATYGEVAEEAGYPGAARAVGNVMA
                     SSEGLPWWRIVRSDGSLAVDKGGEQARRLRAEGVEVRGGRVRPQRSPSV"
     gene            complement(9539..10525)
                     /gene="glpX"
                     /locus_tag="VF097_06485"
     CDS             complement(9539..10525)
                     /gene="glpX"
                     /locus_tag="VF097_06485"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020499281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX6262479.1"
                     /translation="MTEKPDRNLAMELVRTTEAAAMAAGRWAGRGEKESSDQAAVDAM
                     RLMLDTVSMDGIVVIGEGEKDEAPMLYNGEKVGSGDGPLVDVAVDPIDGTRLLSKGMG
                     NALSVVAVSERGTMFDPGAAVYMEKIAVGPEAAEAIDLEAGTAENIARVARAKDVDVD
                     DVTVVVLDRERHEGLVGEIREAGARIRFITDGDVAGAIAAASQRPGADLLMGIGGTPE
                     GVIAAAALKCLGGAIQGRLYPRNDDERSALVDAGYDLDQILTTEDLVSGEEVFFAATG
                     ITDGFLLKGIRYRQREAVTFSMVMRSKSGTIRLVEGRHQLEKLMRFSEIEYD"
BASE COUNT         1231 a         3811 c         3945 g         1587 t
ORIGIN      
        1 cgcggtcgcg ggtcgcttca cacgacccgc gaccgcgccc cctccaggaa cggccctcgc
       61 tccaccagca ccctgacgtg ggtgccccgc gccacgacgc cgcccgggtc gcgcgcctcg
      121 agcgagaaga cgagcctgcg cccgtcgacc tgctcgagcg tggccgtggc ctctatgcgc
      181 gacccgggcg gcgtcggagc caggtgatcg agctcgaccc tcgctccgac cgaggtggtc
      241 ccgggatcga gcgcggtctt gatcgcggcc accgcggccc gctcgacgag ggccagcagc
      301 atcggggtgg cgtagacagg gacgtcgccc gagccgatcc gctcggccgt ctgctcctcg
      361 gccgcggtct cggccaccgt ggcgctggtt ccgggcttca gcatcccctg taggctatcc
      421 cgcgcatgga aggggcatcc ggcgtgatcg cgctcgtgct ggccgagacc gcggccggca
      481 gcgccgccgt tctctggctc acggggctct gggggaacgt gaagcggggc ttcttcatcc
      541 tcaccacggc gaccgtgctg gcctgtgccc tgctggcggc gctgtccgcg aacgccggcg
      601 ccaccgaggc ggggagcggg ggggcgcgcc tcgtggtggt cctggctctc gccctctcgg
      661 cgctttcggg cctcaccctg atcgcgctca tcctgcgccg gatggccgcg gggcgcgtcc
      721 tgggcctcgt ctcgatcccg gtcggggcgg cgctgctggt cgcgatggcg tcggtggcgg
      781 aggcagggat ggcgatggcc ctcgtcgggc tcttcgccgg cgccgccttc atgggtgccg
      841 tcaccgtcgg cctgctgctg ggccactggt acctcgtgga ccggggcctg gcccgcgacc
      901 acatccaacg gatggcggtc gtgctgatcg gggccgtcct ggtcgaggcc gtggccgtga
      961 tcatcgcggc cgtggccgga gaggtgacgc cgacggaggg gttcggcctg ttcctcaccg
     1021 tgggaggcgc cgccttccac ctagccatcg tcctcggcat ggtggcgatc acggccctga
     1081 tcgcgttcct gatccgggcc gcgctgcgcg agacacggcc ccgggccgtc caggccgcga
     1141 cgggcttctt ctatctcgcc gtgatcacgg ctttcaccgc cgagctcgcc gcgaaggtcc
     1201 ggttcctgga ctgagatgga cgccgtcgcg gtggcggtag cgtggatcgg gccgctcgcc
     1261 gtggccggtg cctgggcggc ggtgcgcgtc ggcggggttt cgatctgggc cgcgatgggg
     1321 tggacgttag gccctctggg cgcgctcgcg ctgctgaccg gcggggtgga ggcggggggc
     1381 gggcttctcg aggccttgat cggagtcggg gcggggctcg ctctctacgc ggccaccgcc
     1441 acgttcctct tcctcgcgcg cggatggcgg atcctcaggc ggcaggccca cgagctctac
     1501 gcgcggcgcc gggggctgtc gctcggcgcg gtgctcgcga tcgcgtgcct cgtcgcggtc
     1561 ccgggcgagg agtcgctgtg gcgggggctg atccaggggg gcctgggcga ggctctcggc
     1621 gaggcccccg gcgccgcgct ggcctgggcg ctctacgtcc tggtcaacct cgtgagcggg
     1681 agcgtcccca tcgtgctggg cgcggcggtc ggcggcgccg cgtggacggc cctggccctg
     1741 tggtcgggtg ggatcctggc aagcatcgcc tgccacgtcg tgtgggcggg tctcatgatc
     1801 ctccgtccgc cggtggggga aggctccgac gcatgatctc cgaggaagcc cgccgggtgc
     1861 ttcgagacgg cgtgctcctc tacgccgccg cgcccgggcc ggacgcgccg cacctcaccc
     1921 cgatggtctt cgtcctggcg gaggagcgcc tctgggccac gaccgcgcgc gggtcccgga
     1981 aggttcgcct gtggcgagcg atgccggagg gaggaggcct cgtccgggtc ggcgaccggg
     2041 ccgtgtcgtt ccggggccgc gttgacctct acgacctcct cgaccctacg acgtggggcc
     2101 gctccctgtt ccggagcccc ctgctgacgc gggccgcgct cgggttctcg cggaagaacg
     2161 cgaggttctt cgccggctac gcacgcgacg cccaccgcgt gcccttcggt tggaccccgc
     2221 cgggcagggt cttcctctcg atcgcgctcc gggacggggt cctgctggac ctctcggaag
     2281 gggaggtgct ccgctcctgg gggacgctcg acgagggtct gaccggcgcg gcctcgttcc
     2341 gccgggcgcc ccctcggccc gcccccgacg cgggcgtccc cggcgacgtg ctcgatgccc
     2401 tcggtgacga gggacgaggc gcgttggccc tgggcgacgc tccggtcctc ccggtccgat
     2461 ggaggcgctc ccaggaggag gtcgcgtact acgcggtcgt tccggctccc ttcctggagc
     2521 ggaccggcgc cgcggatcgg gcgcgggcga cgctcgtgct cgaccacctc tcccggtgga
     2581 gggcctcgag gatgctgggg atgcagctcc gaggggaggg acgcgtgtac gtcccggggc
     2641 gtctccgctc gggcggggcg tcgctcgtcg accgctcggg ggccacggag ggcgacgccg
     2701 tggttcgcct tcgccccgat cgcgtcgtgt ggtggcgggg ctgggcgagc ggtagccttg
     2761 gcgcccgatg agtacgatcc gcgacgcggt cgtggtcgac gccacccccg atgaggtgtg
     2821 gcgcgtgatc ggcgacccca ggaaccttcc ccgctggaac cgtcagatca gggcggtcgc
     2881 gggagctccc gatggcgagc tccgcgagga ggactcgtat cgggtccagc tcgggttcct
     2941 gggggtgtcc gccgaggtcc gcgccgtggt gaagaggctg gagcgtccca cctactcgga
     3001 ggtggcgctc agcgggccaa tcgaaggggt ggtgcggacc ttcgtccgcc ccatcggcgc
     3061 cagccggtcg cgtgtggagc acgaggtgga gtaccgattc agagggggag cgctgggccg
     3121 cgcagcggcc cgcgccgtcc ggttgatggg cgcctcgctg atccttcgcc gcggtctccg
     3181 ggcccagaag gagatggtgg aggggagatg aggaggcgcc gtcggagggt gggacgggcg
     3241 ctggcgctcg ccggcgccgc gctgggcctg gcggcgatca ccggctcggt ccgtgggcgg
     3301 gcgttcgacg agcggatcta caagcaggtg aacacgaacc tggagcatcc cacgctcgat
     3361 cggctcctct cgggggtgac tgagctgggt tcgctctacg cgtcgttggg ggccggagcg
     3421 gtcatcgcgt cgtcggggag ccgtcgcgcc gcggcccggg cagtgggggc ggcggtctcg
     3481 atgtgggctc tggggcaggc gctgaagagc tactacaggc gggctcggcc cttcgactcc
     3541 accgcgccgg ggaggctcct gatcgggaag ccccgcggca cctcgtggcc gtccagccat
     3601 cccgcggtct tcctcacctt cgccctggtg gcggcccgtc gcctcgatct ccccggcccc
     3661 gcccgctcgg cccttgtggc gctcgccggt gccgtcggcc tgtcccgcgt ctacctcggc
     3721 gtgcactttc cgtccgacgt cgccgggggg ctgctcctgg gccgggccat ggccgacgtc
     3781 tggacggccc aggacccgga cgtccgctag actcccgccg atggacgcgc tcgccacact
     3841 ctcgtggccc gtgctcgacc gcatcgaact gggcccgctg gcgatctcgc cgcacgggat
     3901 cggcatcgcg gtcggctacc tgctgggctc ttggtggatg atgcgcgacg gtccgaagcg
     3961 gggcctcgag gaggagcacg tcaccacgat catcctctgg gccctggtgg gggcgatcgt
     4021 gggggcgcgt ttcttctatg tcctcggcca cctggatgag ttcgaatctc tcacggacat
     4081 gctcgcgatc tggcgcgggg gcatcagcct cgtgggcggg atcttcgggg cgatcggctt
     4141 cgccgtgccg ctgatgcggc gctacggcta ccgcttcggg caggtgatgg actcggccgc
     4201 ggtcggcctg gccttcggga tcgcggtggg gcggatcggt gacctggtga tcggggacca
     4261 cctgggcaag cccaccgact tcctcctcgg atggcgctac acgagcggga ccttgcctgg
     4321 tccgtatcag gccgccgggc cggagacctg gatcgcgagc ctggccgggc cgcactgcca
     4381 gttgatccgc ccggagggcg ccgagctcta cgaggggacc tgcgatctgt cgggcgccct
     4441 tctggcccag gggcccggtg tccaccagac ggccctctac gacatgctga tcgcgggggc
     4501 cctcttcgct ttcctcttct ggctcagccg gaagccccgc cgcgagggcg tgctgatcgc
     4561 ggcgttcggc atctggtacg gggcgggccg cctgctgacc gacttcctgc gggtggacaa
     4621 gacgtggccg ctggggctca cggggagcca gtgggcctcg gtcggggtga tcctcgcggc
     4681 cgtcgccgtg ctcgtccggt tcgccctgcg accccgtccg gcgcccgtga cgatccctga
     4741 ggcggagacg ggccgggggg agctcccctc cggaccgacg acgagcttca cgcccccgga
     4801 ggagccgcgg ctcaggcctc ccggaggctg agcggcttcc ccctcgggct gcggatccgc
     4861 gtcccgagct cggccgcgat cgcccggaag gcggcccccg cgggggagtc cggctcgctc
     4921 accacgatcg gcgtgccctc gtcggacccc tcgcgcagcg agacctggat cggcaccgat
     4981 ccgaggaggg ggacgcccag cgtctcggcc gtgcgggcgc cccccccctc gccgaacagc
     5041 ggggtgatct cgccgcagtg ggggcagacg aagccggcca tgttctccac gaccccgagg
     5101 acctcgaggt cgatgttggg gttctgggcc atcctccccg cacgctccgc caccttcgcc
     5161 gcggcctcct ggggggtcgt cacgaccagc atcgccccgc gggggaggaa cgaggccagc
     5221 gagatcgaga cgtcgccggt gcccggaggg aggtccacga agagcgcgtc gagctccccc
     5281 cagtagacgt cgcccaggaa ctgctggatc gccttgtgca gcatcgggcc tcgccagatc
     5341 acgggcgtct cgtcggagac gaagaacccc atcgagatga tcttcacgcc gtgggcccgg
     5401 ggcgggatga tcatgtcgct ggtgccggca ggctgtccgc ccacgcccat catccgaggg
     5461 atggagaacc cccagacgtc ggcgtcgagg atccccactc gctggcctgc ggcggcgagc
     5521 gaggccgcga ggttggcggt gacgctcgac ttccccaccc cgcccttccc cgaggcgacg
     5581 gcgatgacct cggttggtcc ctcggagaag aacacggtct cctctctggt cttcccgccc
     5641 cggagccgac cgaccatctc ggcccgctgc tcggggctca tctcgctcac acggagctcg
     5701 aggtgggcgc cgagaccggc cacggcctcc tccaccgagc ggcgcacggc ctcgtcgtcg
     5761 ccggggatcg gcacccggat cgagacgcgc acgcgatcgc gctcgatctc cacggcttcg
     5821 acggccccca gctcgtccag gggcttctcg atgctcggat ccagcgcgcg atgcagcgcg
     5881 tcgcgaacgt cctgctcggt gatcatggct cctcagcgag gtcgcggacg cctccatagt
     5941 atcctggctc gtgcccgctc cgaccccagg ggggcgcatg gacaccaagg ccctcgagat
     6001 ccacaaggcg ctcgccgaca acacgcgctt ccgcctgtac cggcacctgc gcctgatgga
     6061 tcggccggtg tccgtgcggg agatgtcgcg ccgcctctcc ctgcatccca acaccctccg
     6121 gccgcacctc cggcggctgg aggaggccgg gctcgtccgc cacgagctgc gcaagagcga
     6181 cggcgtggga cgcccccaga tgctctacgt gggcgtggag ccggccggcg acgagggccg
     6241 ggatttccgg ctcctttcgg agatgctcct gggcacgatc cagacccggc gtcagctcga
     6301 gcgggccacc gcgctggccc gcggctgggg cgagtacctc gtctcgcggg acgcgccgaa
     6361 gcccggcgtc cgcctccccg accggaagaa cctggcagtc ctgcagtcgg cgatggaccg
     6421 cgcgggcttc gagccgaggt tccggaggca ggggacctcc gtcgaggtca cgttccgccg
     6481 gtgtcccttc cgggagctcg tggacgacca ccgcgaggtg gtgtgcgggc tccaccgcgg
     6541 cctgctcgag gggatgctgg ggggcctgcg cccgcccctc gagctgcgtg agctgaagcc
     6601 gttcgtggag aggggcgtct gcaggctccg cgccgcgccc cctcgcagga agcgcggctg
     6661 atggagccgg ggaatccagg ccggccgccc tcgcgtatca ctggtatggg atcgaccgac
     6721 gcgcccgacg ggccgctgac acccgaggcc gagcgagggc tggtgcggcg gctgatctcc
     6781 ggtgacgagg aggcggtgag gatcctgtac ggtcggttcg gtgggcccgt ctacggcctg
     6841 ggcctgcgcc tcctggggag cccggaggcg gcccaggagc tcacgcagga taccttcctg
     6901 gccgtctggc ggaaggcgcc ccagtacgac ccggcccggg gccggctctc gacctggatc
     6961 atggtgatcg cccacaacct gggcgtggac cgcctgcggc gcgaggcccg ccggccggtg
     7021 gccctggtcg aggacgtgga gaccctggga ggggaggacg tcgagaccga ggctcggatc
     7081 ctggaccgcg tggccaccct ggagttgctc tccgtcctgt ccgccgccga gcggaggctc
     7141 ctgggcctgg cctactaccg gggctggacg gcccgagaga tatctctgca cgactcgatc
     7201 ccgctgggga ccgtgaagac ccgcctgcgg tcggcgctga tcaagctgcg ccgggcccac
     7261 ggatggaagc gggagctctg atggactgcg ccgaggtacg cgggatgctc cccgagcacg
     7321 ccctcgggac gctcggggtg agcgacctcg cccgggtgcg ggagcatctc gccgcctgcg
     7381 ccgggtgccg caaggagacg ggcgagctga tggagggcgt ggaggtggcc gccctcgcca
     7441 ccgccgcccc gcccccgccc gagctcgagg accgcgtcgt ggcggcggtg gcgagggccg
     7501 ccggacgccg ggccggccgc cgccgtctcc cggtcgccgg cctcctggcc gccgcgctcc
     7561 tcctcgcggt cggctggggc gccgcgatgg cgggacgggc cgagcgtctc ggggaccagg
     7621 ccgagatcgc gcgccggcag gccgcgcagg ccgctgggag cttcggggag ttcgtgggag
     7681 acctctccgg cgggggggcc cgcgagacgg tgctccgctc cacgtcgcgg gtgggggccg
     7741 gcgggagggc gatcgtattc gacagcgacg tatccggcgc ctccgactgg gtcctcgtgg
     7801 tcgtgggcgg actgcccgac gaagagggcc cgtaccgggc caagctccgg gggagggagg
     7861 gcgacctccc ggtggggcga ctgtggccgt ccgcggaggg caggctcgcc gcctacgcgc
     7921 tgttcggcgc acttccgggg gacggggagg acgtcgtcgt gctggacgcc gacggcgccg
     7981 tcgtgctcaa gggtcgcctc gaggcgcgct gaggcgccac ccggccgccc gataagatgg
     8041 cccgcatgag gctgggcctc gctcttcccc actacgactt ctcgttcgcg gacggagcgc
     8101 ccgtgacgtg ggagcgcctg cgctcctggg ggaccgccgc cgaggagatg gggttcgact
     8161 cgatctggat ctcggaccac ttcttcctct cgctggcccg ctacgggggg cgggacgagc
     8221 ccttcggcac gcccgaggcc ctcaccgcgc tggccgcgct ggccacgacc acggaccggg
     8281 tgcggctcgg caccctggtg ctgagcgcgg gcttccgcca ccccgcgatc gtggccaagg
     8341 ccgcggccgt catcgacctg ctgtcgggag ggcgcctcga tctggggatc ggggccggtt
     8401 ggtacgagga cgagtaccgg gccttcggct acgagtacga ggacgcccgg ggccggttcg
     8461 acgtcctgga ggagtccctg atcgtcctcg gcgagctcct gggggacggc ccggccacct
     8521 ggcgtggcga gcgattctcg ctggacggcg cctacaaccg ccctgccccg gtccagcgcc
     8581 cgcggccccc gatctgggtc ggcgggaagg gcgggccgcg gctccagcgc ctcgtggccc
     8641 ggcacgggga cggctggaac agcgtctggg cctggtcgcc ggagcggtac cgggagaagg
     8701 tggacgagct ccgagccacc tgcgagcgag aggggcgcga cccggcctcg gtgcggctct
     8761 cggtgggcct ctacctggcg atcggccgcg acgagcgcgg ggtgcgggac gtgctggagc
     8821 ggtacgccga gcgggtgccg gggggcgtgc cggccagcga ggacgtggac acgtggcggt
     8881 cggagcggct ggccggcacg ccccccgagg tcctggagcg cctgggggcc ttcgccgagc
     8941 tcggtgtgga ggaggtgatc gtgtcgccgg cctcggtccc gttcgccgtg cacgacgagg
     9001 atgcgctggc gctcttcgcc caggccgtcc ttcccaccgc ccgcgatctc tgatggacga
     9061 gctcgaggcc gccgtccgcg tcctggagga ggagggggag cccctgcact ggacggtgat
     9121 ccaggacctc gcgctcaagc gtggctacct ggaccccttc acccggaagg acatccggag
     9181 gcggctgatc gccgcgctcg ccgacgccgc caagcgttcc gaccacccga tccggaaggc
     9241 ggggaagggc gtctacacgc tgggggagcg ctgagggcgg acgcggccgc cgcgcacctc
     9301 gacgccctcg gcccggagcc gccgggcctg ctcgcccccc ttgtccaccg cgaggctgcc
     9361 gtcggagcgg acgatccgcc accaggggag cccctcgctg gacgccatca cgttgcccac
     9421 cgcgcgggcc gcgcccgggt agccggcctc ctcggccacc tcgccgtagg tggcgacctc
     9481 gccggggccg agcgcgcgga cgaccacggt caccgcctcc tcgaacgacc ccacctcgtc
     9541 agtcgtactc gatctccgag aagcgcatca gcttctcgag ctggtggcgg ccctcgacca
     9601 gccggatcgt ccccgacttc gaccgcatca ccatcgagaa cgtcaccgcc tcgcgctgcc
     9661 ggtagcggat ccccttcagc aggaagccgt cggtgatgcc cgttgccgcg aagaagacct
     9721 cctcgccgga gacgaggtcc tcggtggtga ggatctgatc gaggtcgtac ccggcgtcca
     9781 cgagggcgct ccgctcgtcg tcgttcctcg ggtagagcct gccctggatc gcgccgccca
     9841 ggcacttcag ggcggccgcc gcgatcaccc cctccggcgt gccgccgatc cccatgagga
     9901 ggtccgcgcc gggcctctgg gaggccgcgg cgatcgcgcc cgcgacgtcg ccgtcggtga
     9961 tgaagcggat ccgggctccc gcctcgcgga tctccccgac caggccctcg tgccgctccc
    10021 ggtccagcac gacgacggtg acgtcgtcca cgtccacgtc cttcgccctc gccacgcggg
    10081 cgatgttctc ggccgtgccg gcctcgaggt cgatcgcctc ggccgcctcg ggccccacgg
    10141 cgatcttctc catgtacacg gcggcccccg ggtcgaacat cgtgcctcgc tcggacacgg
    10201 ccaccaccga gagcgcgttc cccatcccct tggagaggag gcgggtcccg tcgatcgggt
    10261 ccaccgcgac gtcgaccagc gggccgtcgc cgctccccac cttctcgccg ttgtagagca
    10321 tcggcgcctc gtccttctcg ccctcgccga tcacgacgat gccgtccatg gagacggtgt
    10381 cgagcatcag gcgcatcgcg tccaccgccg cctggtcgga cgactccttc tcacctcggc
    10441 cggcccagcg gccggcggcc atcgccgcgg cctcggtggt ccgcaccagc tccatcgcca
    10501 ggttccgatc gggcttctcc gtcatcgcag ctcctccaag agactcgtgg gggacgctcc
    10561 ctcgtggagg atgc
//
LOCUS       DASUTZ010000148        10357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_431313, whole genome shotgun sequence.
ACCESSION   DASUTZ010000148 DASUTZ010000000
VERSION     DASUTZ010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10357
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431313"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3877)
                     /locus_tag="VF097_06490"
     CDS             complement(<1..3877)
                     /locus_tag="VF097_06490"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX6262480.1"
                     /translation="MVAIAVVITLNRYMDHDPDGRMYVLEQDLDEVRREEAANDRART
                     TGSDPAVSLGLQGDAIQPLTLRVLPGECLMVRLRNDLPGQEAATFHVHSSQLVVEGTG
                     RPAIATEPSAVVLPGQTVTYAWAVPADEPEGTHYVHSHGDTRRQTSHGLFGSVVVEPP
                     GSSWRDPWSDDAATTSWSAIVEPRNAPAFREFVLYYHEVGTESEVPFGADGAELPLVD
                     PFTNSYKPSGRALNYRSEPFLHRLQRQSDLGGTPDESIGYSSYAFGDPATPIMRAYLG
                     DAVKQRVVHAGSEVFHVHHVHGGSIRWPRQPGLDPSPPIIGLEKHPPLLPAVSERTDS
                     QSIGPSEVFDVEPECGAGGCQQSAGDVLFHCHVAHHYISGMWGIWRVYNTLQDGAVST
                     DQLPPLPELPDHRGVRPAVTSDELPQELRDGIEDLLPPKGTPGDGDASVWDWSIRRGV
                     YVGEPEDTRSWEGHTATRSGRPPLLFDPRDGRLAFPHLRPHLARRPPFAPGHGPAPYL
                     DPGGGSPNVPRPGENGDRSVCPRGSRVRDLDITAVDMPIPLNTEENLVDPAGMLFIRR
                     EQHDLVLADAAHRVPLVLRTNASVDCVDVTLTSEVADDADNGWLSKVNAHIHFVQFDV
                     QGSDGVDAGFNHEQSIRPWTVESERLTRAVAAGATRLFVAETERFRPGALVMVGVDTT
                     DRAEVRTIEAVEDGALRLAEPLENDHAARERVTVEFVRYRWYPDVQFGTAFFHDHVNA
                     IFSGRHGLWGALVAEPPDATWHDPYTGREVPSGAVADIYTTGSVSGDVSGSFRELVAF
                     VQDDNPITKEGRSSGSSLGLRVEPLDPRTEDDPAVAFDSFRHGDPETPILRARPGDPI
                     VLRTVVSATNDVHTIHIDGHAFRSEPWSETSPPTSTAHLGISERLDLVIPSAGGPARL
                     PGDYLIRNGRASKLREGSWGLLRVLPREHPDAPRALPGVRAGPIGDQVCPSEAPMRSF
                     DVTAVEAPLPMLNGDLGMAFVPTGSADAVASGARVPEPLVLRATVGDCLEVLVRNATD
                     RAVSFDPDLMVFDPGERAVGRNGGGPIPPGEQRVSRFHAHPDFGEAMVLVLDGGDPVE
                     GPALGGFAAIALAEAGAVFLDPSSGREMSAGWVVDVIEPGGTAYRDAVLFLQEEDAAI
                     GTHLMPYRSDVQGTVGINYRFAPLADRLLLDPDPAHAFSSAIHGDPQTPVVEAYAGDP
                     IRLRVAAPLSEQGQVFTVEGHRWRLEQRPGAMIASSIQLGALEAVTIELDGGAGGRAQ
                     LPGDYLYGDHREPYREAGLWGLLRVHDPCA"
     gene            3974..4096
                     /locus_tag="VF097_06495"
     CDS             3974..4096
                     /locus_tag="VF097_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262481.1"
                     /translation="MSVHRPRGILGVVIVPVSVWMDVSELVRGIGVHVVRTRSR"
     gene            complement(4212..5330)
                     /locus_tag="VF097_06500"
     CDS             complement(4212..5330)
                     /locus_tag="VF097_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262482.1"
                     /translation="MRPIVAERRRSRHSGRDRRRNRLAGVFAVGLIAAGCGGTGTTDA
                     PETQEPPEGERASFSVGVGGSVEEFNGNVLSFLPDSLKVHPGDAVVFRNLETGEPHTV
                     TLGTDAPTSETLPGGWFFDGGFGGPPRQDTSMPCFLETGLPPVAGECPPGQREPVPFD
                     GTQSWFNSGVLVEEEDFTLQLADDIAPGAYPFVCLVHVAEMRGFINVVEPGQPADDPA
                     DVIAQAEEQLEREVNVLRPALDRSPEKNEVFAGLTEPRLIAWANVFRPEDVQIPVGGT
                     VTWRAVGLHVIAFNAPESASPFYQRAPDGSLTENPMSGDSSGRFVEWNGKGLLTSGLM
                     EGVPQSPAVFSVTFTRAGTYPYQCLVHFDMEGRVKVGG"
     gene            5511..10265
                     /locus_tag="VF097_06505"
     CDS             5511..10265
                     /locus_tag="VF097_06505"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6262483.1"
                     /translation="MEFRLLGRLHVSERGRAIPLGGPKQRVVLAHLLLRSNEIVSADR
                     LIDEVWGADPPAAARSTVWGYVSHLRRALGSDRLKGGTGGYVLHADPSEIDAHRFEAL
                     VEEARRQMATDQAVAASTYSKALDLWRGPVLEDVSGRSSLTPEITRLEELRLAAMEER
                     IGAELDLGRHAALVPDVTTLVEAHPTRERLWGYLMTALYRSGRQAEALTAYRRARDLL
                     ARELGIDPTPDLRRLHEQILRQDGALEIVGAPLRGYRLLEQIGAGPLGVVHRALQPQV
                     GGREVAIKAIHAGFADDPRFIRRFEAEAQLVARLEHPHIVPLYDFWRGPGSAYLVTRY
                     LRGGSLEERLAEGPMAPEEAARLCDHVAAALASAHRQGVIHRDVTPANILFDEDHNAY
                     VSDFGIARDLVVAMAASPGERRGHLTHHLSPEEIRGEPVTPRTDVYGLGLVLLEVLAG
                     CRAFSGSHTAELIEDRLNDTLPSIGSLNARLPTGVGEVIERATATDSDARYGDPAGLA
                     AAFRDALFPAARPVPVPGAEAAHNPYKGLRPFFEADAADFFGRETLVTELVTRLAERG
                     RWSRFLAVVGPSGCGKSSLVRAGLVPALRGSRVPGSDAWFTIEMHPGDNPFQELNAAL
                     MRVAVNRAQGFMERLERGDEGLVRAAEEALPQGTELLLVIDQFEEIFTLTSDEDRARF
                     LRLLHTAAVDPESRLRIVVTLRADFYDKPLAYPGLAELVKARSVTVTPLTPVELERAV
                     AGPAERVGVEVDAQLVAEIVADVSVHPSSLPLLQYALTELFDQGSQATLGLDGYRKIG
                     GVSGALARRAKDLYDGLDERGKEAARQLFLRLVVLGEEGDEDTRRRVLRAELTSLEAD
                     RALMDMIIDTFGDRRLLAFDRDPVTRGPTVELSHEALLREWERLRGWIEAAREDLRAH
                     RRLASAAMEWAGAGRDPSFLLRGARLARFQDWSATAGIALTGDERAYLEISLAQGQAE
                     RAEEEARRTRESQLEKRSIVRLRALVLVLALAVLLASGLSIFAFGQQGEASRQRDLVA
                     ERSVLSRARELVLFSNAALNSDPELSLLLALESIELTAVEKGSVLPEAVDAMHWALQG
                     MRVQFPTGPPVAVRAGPDGVRGVFDLPLEELMPFARSRLERELTEGECRRYLELDHCP
                     APRDLGDLSELSLFAPEAMGQGMAPGESSLAGTQVRVATYFQDAQLAGWNRELERFRE
                     ATGIDPITVPQPSGVGAPGAEVEADIVLLPRLGDLQPLWRAGALMDIGAYLDMNRIRR
                     DYAPHLVHLTTIGDDGTWPAESGTLVAGWVSLNVKSLIWYPVPAFEPAGYTIPRTWDE
                     LIALSDRLLADGRTPWCFGIESGNATGWVATDWVEELVLRTAGLETYDRWVAHEIPFT
                     HPAIKRAVELMGRIALSPGYVAGGGDAIVRTSFWRAQNRMFEDPPACWLHRQASFAPV
                     GFPRGRVAGIDSAAFPFPAMDVDDPPVLLGAGDVFGVFADRPEVREFVRFLVSPDFGR
                     AWARMPEGAYISPNLRFGLDAHGSEVTRAQAEAARRALERDTFRFDASDSMPSEVGSG
                     AFFSGMTEYLWLGPDNLDRVLARIEESWPQQD"
BASE COUNT         1586 a         3573 c         3584 g         1614 t
ORIGIN      
        1 cggcgcacgg atcatgcacg cgcaggagac cccacaaacc ggcctcccga tacggctcgc
       61 gatggtcgcc gtataggtag tcgccgggca actgagcccg accaccggcg ccgccatcga
      121 gctcaatggt caccgcctcc aaggcgccga gctggatcga actcgcgatc atcgctccag
      181 gccgctgctc caaccgccag cggtgacctt cgacggtgaa gacctgtcct tgctcactga
      241 gtggagccgc cacgcgcaaa cggatcgggt cgcccgcgta ggcctcgacg acgggcgttt
      301 ggggatcacc gtggatcgcg ctgctgaacg cgtgggccgg gtcggggtcc aggagcaggc
      361 gatccgccag cggggcgaac cggtagttga tccccaccgt tccctgaacg tcgctgcggt
      421 acggcatcag gtgggtgccg atcgcagcgt cttcctcctg caggaacagc accgcatcgc
      481 ggtaggcagt cccccccggc tcgatgacgt cgaccaccca cccagccgac atctcccgac
      541 cgctggaagg atcgaggaag acggcaccgg cctcggctaa tgcgatggct gcgaaccctc
      601 cgagcgcagg accctcgacc gggtccccgc catcgaggac cagcaccatg gcttccccga
      661 agtccggatg tgcgtggaag cgcgagacgc gctgttcgcc cggcgggata ggccccccgc
      721 cgttgcggcc gacggcgcgc tcgcccgggt cgaagaccat cagatcggga tcgaaggaaa
      781 ccgcgcggtc agtcgcgttg cgaacgagga cctcgaggca gtcccccacc gtcgccctga
      841 ggacgagcgg ttccggcaca cgagctcccg aggccaccgc gtcggcggat ccggtcggca
      901 cgaaggccat tccgaggtcg ccgttcagca tgggcagcgg cgcctcgacc gcggtgacgt
      961 cgaacgaacg catgggagcc tccgatgggc agacctgatc gccgatcggg ccggcgcgca
     1021 cccccggcag agcccgcggc gcatcgggat gttcccgagg caacacgcgg agcagtcccc
     1081 acgacccttc tctcagcttc gacgcccgac cgttgcggat caggtagtcc cctggtagcc
     1141 gcgccgggcc gccggccgag gggatcacca ggtccagccg ttccgagatg cccaggtgtg
     1201 cggtggaggt gggcggtgac gtctccgacc acggctccga tcggaacgcg tgtccgtcga
     1261 tgtggatggt gtgcacgtcg ttggtcgccg agaccacggt acgcaggacg atcgggtcac
     1321 cgggacgagc ccggaggatg ggcgtctccg gatcgccgtg gcggaaggag tcgaacgcga
     1381 ccgcgggatc gtcctcggtt cggggatcga gcggctcgac gcggaggccc agcgagctcc
     1441 ccgacgaccg cccctccttc gtgatcgggt tgtcgtcctg aacgaacgcg acgagttccc
     1501 ggaagcttcc cgacacgtcc ccggagaccg acccggtggt gtagatgtcg gccaccgccc
     1561 ccgaggggac ctccctgccg gtgtaggggt cgtgccacgt cgcgtccggc ggctccgcga
     1621 ccagcgctcc ccacagcccg tggcgacccg agaaaatcgc gttgacgtgg tcgtggaaga
     1681 acgcggtccc gaactggaca tcggggtacc agcggtaccg gacgaactcg acggtgaccc
     1741 gctcccgtgc cgcgtggtcg ttctcgagcg gctcggcgag acggagcgcg ccgtcctcca
     1801 cggcttcgat cgtgcggacc tccgcccggt cggtggtgtc gacaccgacc atcacgagcg
     1861 caccgggccg gaaccgttcg gtctcggcca cgaaaagtcg agtggcgccg gcggcgacgg
     1921 ccctggtgag ccgttcgctc tcgaccgtcc aggggcggat ggactgctcg tggttgaagc
     1981 cggcatcgac cccgtcgctt ccctgcacgt cgaactggac gaagtggatg tgcgcgttga
     2041 ccttgctgag ccaaccgttg tcggcgtcat cggcaacctc gctcgtgagc gtcacgtcca
     2101 cgcaatcgac cgaagcgttc gtccggagaa ccagagggac ccggtgcgcc gcgtcggcga
     2161 ggaccaggtc gtgctgctcc cttcggatga acaacatccc ggccggatcc accaagttct
     2221 cctccgtgtt caacgggatc ggcatgtcga cggcagtgat gtcgaggtcc cgcacacgcg
     2281 agcctcgcgg gcacacgctc cgatccccgt tctcaccggg ccggggcacg ttcgggctgc
     2341 cgcccccggg gtccaggtac ggagccggcc cgtggcccgg cgcgaaggga gggcgacggg
     2401 cgagatgcgg tcgcaggtgc gggaaggcca ggcgtccgtc gcgcgggtcg aacaggagcg
     2461 gcggccggcc gctcctcgtc gcggtgtgcc cctcccagga cctcgtgtcc tcgggctcgc
     2521 cgacgtagac acctcgtcgg atcgaccagt cccacacaga ggcgtcccca tcgccggggg
     2581 ttcccttcgg tggcaggaga tcctcgatcc catctcgcag ctcctggggc agctcgtcgg
     2641 aggtgacggc cgggcgaacc ccacggtgat ccggcagctc gggcagcggc ggcagctggt
     2701 cggtcgagac ggcgccgtcc tgcagcgtgt tgtagacgcg ccagatgccc cacatccccg
     2761 agatgtagtg gtgggcgacg tgacagtgga acaggacgtc tcccgcactc tgctggcatc
     2821 cgccggctcc acattccggc tcgacgtcga agacctccga gggtccgatc gattgcgaat
     2881 ccgtgcgctc ggagacagcc ggaaggagcg gtgggtgctt ctccaggccg atgatggggg
     2941 ggctcggatc caggccgggt tgccggggcc accggatcga acccccgtgc acgtgatgga
     3001 cgtggaacac ctccgatccc gcgtgaacga cccgctgttt gacggcatcg ccgaggtagg
     3061 cccgcatgat cggtgtggcc gggtcgccga aggcgtagga gctgtagccg atggattcgt
     3121 cgggcgttcc acccagatcg gactggcgct gcaatcggtg caggaacggt tcactccgat
     3181 agttgagcgc ccgtccggac ggcttgtacg agttggtgaa cggatccacc agcggcagct
     3241 ccgcgccgtc ggctccgaac gggacctcgg actcggtgcc gacctcgtgg tagtagagga
     3301 cgaactcgcg aaacgccgga gcattcctcg gttcgacgat cgccgaccac gaggtcgttg
     3361 ccgcgtcgtc cgaccacgga tcgcgccagc tcgagccggg aggctccacc accacggaac
     3421 cgaacaggcc gtgagacgtt tggcgccggg tgtcaccgtg actgtgcacg tagtgggttc
     3481 cctcgggctc gtcggccggg accgcccacg cgtacgtgac ggtctgcccg gggaggacga
     3541 cggcagaggg ttcggtggcg atcgccggtc gaccggtccc ctccaccacg agctgcgagg
     3601 aatgaacatg aaaggtcgcg gcctcttgcc ctggcagatc gttgcgcaac cgaaccatca
     3661 agcactcacc gggaaggacc cgcagggtca gtggctggat cgcgtcgccc tggaggccga
     3721 gcgacacggc aggatcggac ccggtcgttc gggcgcgatc gttggccgcc tcttcgcggc
     3781 gcacctcgtc gaggtcctgc tcgaggacgt acatacgtcc gtcgggatcg tgatccatgt
     3841 accggttgag ggtgatcacg accgcgatgg cgaccacgtc atatcgacgg acgggcgcct
     3901 cgtccgggca acgcccaccc ggatccaccg actcgatccc ggaaggggcc aacacaaggc
     3961 cctccgagcc ttcgtgagcg tccatcgccc ccgtgggatc ctgggcgtcg tgatcgtgcc
     4021 cgtgtccgtc tggatggacg tgtccgagct tgtacgcggg atcggcgtcc acgtcgtgcg
     4081 gaccaggagt cggtgaggac atggcggaca ggccgaacag cgccatcgca aagatcacgg
     4141 cccatggtcg ccggcccgga tcgcggatgg gactcaacga tcccggcgcc cctcgaggcc
     4201 gggacgcgat gtcacccacc caccttgacc ctgccttcca tgtcgaagtg caccaagcac
     4261 tggtacgggt aggtgccggc gcgggtgaag gtcacgctga agaccgccgg cgattgaggt
     4321 acaccttcca tcaagcccga ggtcaacagc cccttcccgt tccactccac gaatcggccg
     4381 ctcgaatctc ccgacatcgg gttttccgtg aggctcccgt ccggggctcg ttggtagaag
     4441 ggtgaggcgc tttccggagc gttgaaggcg atgacgtgta ggcccactgc cctccaggtg
     4501 accgtgcctc cgaccgggat ctggacatcc tcgggccgga agacgttggc ccaggcaatg
     4561 agccggggct cggtcaaccc agcgaagacc tcgttcttct ccggcgagcg atccagcgcg
     4621 ggccgcagga cgttgacttc tcgctcgagc tgctcctccg cctgggcgat cacgtctgcc
     4681 ggatcgtcgg cgggctgacc cggctccacg acgttgatga accctctcat ctcggcgacg
     4741 tgcaccaggc acacgaacgg gtacgcgccc ggcgcgatgt cgtcggcgag ttggagggtg
     4801 aagtcctctt cctctacgag aacgccgctg ttgaaccacg actgggtccc gtcgaacggc
     4861 acgggttcac gttgcccggg ggggcactcc ccagcaaccg gggggaggcc cgtttcgagg
     4921 aagcacggca tgctcgtatc ctgacgcggc gggccaccga atccgccgtc gaagaaccac
     4981 cctcccggca gggtctcgga cgtcggcgcg tccgttccga gcgtcaccgt gtggggctcg
     5041 ccggtctcca ggttgcgaaa gacgacggcg tcgcctgggt gcaccttgag cgagtccggg
     5101 aggaaggaga ggacgttacc gttgaactcc tcgacggacc cacccacgcc gaccgagaac
     5161 gaggcgcgtt ccccctcggg cggctcctgg gtctcgggcg cgtccgtcgt tcccgttcct
     5221 ccgcacccgg cggcgatgag cccgacggca aacaccccgg ccagccggtt tcggcgacga
     5281 tcccgcccgc tgtggcgcga tctgcgcctc tcggcgacga taggcctcat gttcgtcacc
     5341 tcccgtagcc cgcggtcggc gggagcatcc aggccagcgg gacggccccg atctgccccg
     5401 ctcggtgagg ggtcgcgcgg acaggatgga gccgtcgcct tggaagagcc ttggaaccga
     5461 cgagggctcg gttattccac cgctgcgccg gccgaaggag cgtgacgacc atggagttcc
     5521 gcctcctcgg ccggctccat gtgtccgagc ggggccgggc gatccccctg ggaggaccga
     5581 agcagcgagt cgttctcgca catctcctcc ttcggtcgaa cgagatcgtc tccgccgacc
     5641 gcctgatcga cgaggtgtgg ggggccgatc cccctgcggc cgcccggagc acggtctggg
     5701 gctacgtctc gcacctgcga agggccctgg gttcggaccg gctgaaaggt gggacgggcg
     5761 gctacgtcct ccacgccgac ccctcggaga tcgacgcgca tcggttcgag gccctggtgg
     5821 aggaagcgag acggcagatg gctacagacc aggccgttgc cgcgtctacg tattcgaagg
     5881 ccctcgacct ctggcgaggc ccggtcctcg aggacgtctc gggccgatct tccctcacac
     5941 cggagatcac tcgcctggag gagctacgac tggcggcgat ggaggaacgg atcggcgcgg
     6001 agctggatct cggacgccac gcagcgctcg tccccgacgt gacgaccctc gtcgaggccc
     6061 atccaacgag agaacggcta tggggctacc tgatgaccgc cctgtaccgc tcgggacgcc
     6121 aggccgaagc gctcaccgcc taccggcggg cgcgggacct cctcgcgcgt gagctgggga
     6181 tcgaccccac gcccgatctc cggcgcctcc atgagcagat ccttcgtcag gacggcgccc
     6241 tggagatcgt cggtgcgccg cttcgcggct accggttgct cgagcagatc ggggcggggc
     6301 cccttggcgt ggtccaccgg gccctccagc cgcaggtggg aggccgtgag gtcgcgatca
     6361 aggccatcca cgcggggttc gccgacgacc cccgattcat ccgtcgcttc gaagccgaag
     6421 cgcaactcgt ggctcgcctg gagcaccccc acatcgtccc cttgtacgac ttctggcggg
     6481 gaccggggag tgcctacctc gtcacgcgct acctgcgagg gggaagcctg gaagaacgcc
     6541 tcgccgaagg acccatggcc ccggaagagg cggcgcggct ctgcgaccac gtggcggcgg
     6601 ccctcgcatc ggcccaccgc caaggcgtga tccaccgcga cgtcacaccg gccaacatcc
     6661 tcttcgacga ggaccacaac gcgtacgtgt cggacttcgg aatcgccagg gacctcgtcg
     6721 tggccatggc ggcctcaccg ggtgaacgcc ggggccacct cacccaccac ctctctccgg
     6781 aggagatccg cggcgagccg gtcaccccga gaaccgacgt ctacggtctg ggcctcgtcc
     6841 tcctcgaggt cctcgcgggc tgccgcgcgt tctccggcag ccacacggct gagctcatcg
     6901 aggaccgtct gaacgatacc cttccttcga tcggctccct gaacgccagg ctcccaacgg
     6961 gcgtcgggga agtcatcgag cgggccaccg ccaccgactc ggacgcacga tacggagatc
     7021 ccgcggggct cgccgccgcc ttccgagacg cgctgttccc cgccgctcgc cccgtcccgg
     7081 ttcctggggc tgaggcggcg cacaacccct acaaaggcct gcggcccttc ttcgaggccg
     7141 atgccgccga cttcttcggc cgggagacgc tcgttaccga gctcgtgacc aggctcgccg
     7201 aacgcggaag gtggtcacgt ttcctggcgg tcgtcgggcc gtccggatgc gggaagtcct
     7261 ccctggttcg ggccggcctg gtgcccgccc tcaggggcag ccgggtaccg ggctccgacg
     7321 cgtggttcac catcgagatg catccggggg acaatccgtt ccaggagctg aacgccgccc
     7381 tcatgcgcgt cgccgtcaac cgagcgcagg ggttcatgga gcgcctggag cgcggtgacg
     7441 aagggctcgt tcgcgccgcg gaggaggccc tgccccaagg gacggagctt cttctggtca
     7501 tcgatcagtt cgaggagatc ttcaccctca cgagtgacga ggaccgggcg aggttccttc
     7561 ggctcctgca caccgcggcc gtcgatccgg agagccggct gaggatcgtc gtcaccctgc
     7621 gggccgactt ctacgacaag ccgctcgcgt atccggggtt ggccgagctg gtgaaggccc
     7681 gatcggtgac cgtgacccct ctgaccccgg tggagttgga gcgtgcggtc gccggtcccg
     7741 cggagcgggt gggtgtcgag gtggatgcgc aactcgtggc cgagatcgtg gccgatgtgt
     7801 ccgtgcaccc gagctccctt ccgctcctgc aatacgcgct caccgagctg ttcgaccagg
     7861 ggagccaggc gaccctggga ctcgacggtt accggaagat cgggggcgtc tccggggccc
     7921 tcgcccggcg ggccaaggac ctgtacgacg ggctcgacga acgtggaaag gaggcggccc
     7981 ggcaactctt ccttcgcctc gtggtcctgg gcgaggaggg cgacgaggac acccgccgcc
     8041 gcgtcctccg ggccgagctc acctccctcg aggcggatcg agcgttgatg gacatgatca
     8101 tcgacacctt cggcgaccgc aggctcctcg ccttcgaccg cgacccggtg acccgtggac
     8161 cgacggtgga actctcgcat gaggccctgc tccgggagtg ggagcgcctc cgagggtgga
     8221 tcgaggccgc tcgggaagac ctgcgggcgc accggcgact cgcctcggcg gccatggagt
     8281 gggccggcgc cggccgggac ccgagcttcc tgctccgcgg agcccgcctc gcgcggttcc
     8341 aggactggtc ggccaccgcg gggatcgcgc tcacgggcga cgagcgcgcc tacctcgaga
     8401 tcagcctggc gcagggccag gccgagcggg cggaggagga ggcgcggagg acccgagagt
     8461 cgcagctgga gaagcgctcg attgtccgcc ttcgggccct cgtcctcgtc ctggcgctgg
     8521 ccgtgctcct ggcgagcggc ctgtccatct tcgcgttcgg ccagcaggga gaggccagcc
     8581 ggcagcgcga cctcgtcgct gagcggagcg tgctctcccg cgcccgggag ctggtgctct
     8641 tctcgaacgc ggcgctcaac agcgacccgg agttgagcct gttgttggcc ctcgagtcga
     8701 ttgagctgac ggccgtcgag aagggctccg tcctgccaga agcggtcgac gccatgcact
     8761 gggcgttgca ggggatgcga gtgcagttcc cgaccggccc tcctgtcgcc gtccgggcgg
     8821 gtccggacgg ggtccggggc gtgttcgatc tgccgctcga ggagctcatg ccgttcgcca
     8881 ggagccggct cgagcgagaa ctaaccgagg gtgaatgccg ccgctatctc gaactcgatc
     8941 actgccctgc tccccgcgac ctcggcgacc tatccgaact ctccctgttc gcccccgagg
     9001 cgatgggcca agggatggcc ccaggcgagt cgtcgctggc cggtacccag gtgcgtgtcg
     9061 ccacgtactt ccaagatgcc cagctggcgg gttggaaccg ggagctcgag cggttccggg
     9121 aggcaaccgg catcgacccg atcacggtgc cgcagccctc cggggttgga gcgccgggcg
     9181 cggaggtcga ggcggacatc gtgctcctgc cgaggctcgg ggatctccaa cccctctggc
     9241 gtgccggcgc gctgatggac atcggggcgt acctcgatat gaacaggatc cgccgcgact
     9301 acgctccgca cctggtgcac ctgaccacta tcggtgacga cgggacatgg ccggccgagt
     9361 cgggaaccct cgtcgccggg tgggtgagcc tcaacgtcaa gagcctgatc tggtacccgg
     9421 tgcccgcctt tgaaccagcc gggtacacga tcccgcggac ctgggacgag ctgatcgcgc
     9481 tcagcgatcg tctgctcgcc gacggccgta ccccctggtg cttcggcatc gagagcggta
     9541 acgccaccgg ctgggtggcc acggactggg tcgaggagct cgtgctccgg acggcgggcc
     9601 tcgagacgta cgaccggtgg gtggcgcacg agatcccgtt cacccacccg gcgatcaaac
     9661 gcgccgtcga gctcatgggg cgcatcgctc tttcgcccgg gtatgtggcc ggagggggtg
     9721 atgcgatcgt gcgtacttcc ttctggcgtg ctcagaaccg catgttcgag gatcccccgg
     9781 cgtgctggtt gcaccggcag gccagctttg cccccgtcgg attcccgcgc ggccgggtcg
     9841 ccggcatcga ctcggcggcc ttcccgttcc cggcgatgga tgtcgacgac ccgcccgtcc
     9901 tcctcggcgc gggtgacgtc ttcggggtgt tcgccgaccg accggaggtg cgtgagttcg
     9961 tgcggttcct ggtcagcccc gacttcggca gggcgtgggc gaggatgcct gaaggcgcgt
    10021 acatctcccc gaacctgcgg ttcggcctcg acgctcacgg gagcgaggtc acgcgcgcgc
    10081 aggctgaggc tgcccgtcgc gccctcgagc gcgacacgtt ccgattcgat gcctccgact
    10141 cgatgccttc ggaggtcggc tccggagcgt tcttctcggg catgaccgag tatctgtggc
    10201 tcgggccgga caacctcgat cgggtgctgg cccgtatcga ggagagttgg ccccagcagg
    10261 actgagctgg gctcagcgct cgggcgccgg gtattcgaag tgcatgccgt ccggtcgaac
    10321 gcgcggacca tgcgggtcct ggttcgtggg gggcaca
//
LOCUS       DASUTZ010000149         9645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_821265, whole genome shotgun sequence.
ACCESSION   DASUTZ010000149 DASUTZ010000000
VERSION     DASUTZ010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9645
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821265"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(537..4064)
                     /locus_tag="VF097_06510"
     CDS             complement(537..4064)
                     /locus_tag="VF097_06510"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HEX6262484.1"
                     /translation="MKRSDFGPNTWLVEQMYRRYQENPSSVSEAWQEFFEDYVPETDG
                     QRRPATPREEAPPEPEPKEEVPEEAVPLRGAAKIIAENMDRSLGVPTATSFRTVPAKL
                     LEVNRGMLNRHLGRNRGGKVSFTHLIGFAVVRALAERPAMNVAYREIDGTPHAIRNPH
                     VHLGLAVDVQKEDGSRTLLVPNIKEAETLEFDAFWRSYEEMVEKVQRNELRPDDFAGT
                     TVSITNPGMLGTVQSVPRLMPGQAAIIGVGAIGHPAEYSGADPDTLAELGVGKVLTLT
                     STYDHRVIQGAESGEFLRDVERLLLGGEDFYDRVFTSMGVPYVPVEWRRDVNPRPGTL
                     GADEKEARVSQLINMYRVRGHLIANLDPLESEPPAMHPELDPAFFGFTIWDLDRRFPT
                     GGLAGTEEMSLGEILTVLRDAYCRTSTVEYMHMQDVEPKRWIQERMEGPQADLTKEDR
                     TYILSKLNQAEAFEQFLHTKYVGHRRYGLEGAESMIPMLDAILDLAADSAIEETLIGM
                     AHRGRLNVLANVIGKSYAQIFRQFEGDIDPLSSQGSGDVKYHVGAAGKHTSRAGNQLV
                     VSVVSNPSHLEAVDPVLQGMARAKLEQLGRDDEDPVLPLLIHGDAAFAGQGVVAETFA
                     LSQLQGYSTGGTVHIIVNNQLGFTTGSDYGRSSTYATDVAKMVQAPIFHVNGDDPEAC
                     VRVARLAFAFRETFHRDVVIDMWCYRRWGHNEGDEPAFTQPLMYEKIRSLRSVRKRYM
                     EQLVNRGLLTVEEAERSLEEYRETLQQALDETKAEPQPQRPSAREAREAEPAPPIETG
                     VDRAVLDEVIEGIATVPETFHVHEKLQRWLDQRRKALPEDAVDWSLAEALAFGSLIRD
                     GVWVRLSGQDTRRGTFSQRHSVLVDQETGQEYLPLANLEGAPGRFVALDSLLSELAAL
                     GFEYGYSVSNPGALVLWEAQFGDFANGAQVVIDQFIASAEDKWGQTSGLVMLLPHGFE
                     GQGPEHSSARLERYLTLAAKDNMEVAVPSTPAQYFHLLRRQALRDRRKPLIVLTPKSP
                     LRLPAARSPADEFTQGRFREVLPDPSGPSEARRVLLCQGKFFYDLLRGREEREVDDVA
                     LVRVEQVYPFPAAALREQLDRYPGAEVVWAQEEPENMGAARFVHLKMERVLDVRPRLV
                     ARPESPSPATGSQTVHQQEQAELIDRAIS"
     gene            complement(4132..4932)
                     /locus_tag="VF097_06515"
     CDS             complement(4132..4932)
                     /locus_tag="VF097_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017614120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6262485.1"
                     /translation="MRFAIHAPNFGAPSDLLDLARAAEASGWDGFFLWDHVGSPAEAP
                     MEVLDPWVLLGAIAVRTERIRVGTAVTPLARRRPHVVAREAATLDVLSGGRAVLGVGL
                     GNPPDAEFAAFGEPADAQHRAPLLDEALHLIAALWTGEPVNHEGPGYTARDVAFLPRP
                     VQRPRIPIWAACALPHRAPLRRAARWDGVLLSGPVDGDLSPPSPPGVRAAADELEHLA
                     GAPRDVAVVAAGPPPDPAAYRQAGATWWLATGWPAQLAEMASQPPPRA"
     gene            complement(4937..5446)
                     /locus_tag="VF097_06520"
     CDS             complement(4937..5446)
                     /locus_tag="VF097_06520"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012834970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEX6262486.1"
                     /translation="MARITLAQGDLTEQELDAIVNAANSSLMGGGGVDGAIHRKGGPA
                     IFEACKRIRAETYPNGLPTGQAVATPAGNLPARWVIHTVGPVYAKSEDRSELLASCHT
                     ESLRVADELGARTVGFPAISTGVYGYPVEEAAPVAIAAVREADTQVDEVRFVLFDVAT
                     YRAFERALG"
     gene            5470..6618
                     /locus_tag="VF097_06525"
     CDS             5470..6618
                     /locus_tag="VF097_06525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6262487.1"
                     /translation="MPDVIVVGGGVIGAACAREAARRGMSVTLVERSELAAGASGRNH
                     GLLLTPPDPALVPMFHASRELYVEAADGAAVPFDLDPKPLGFLIVATDDQGERAAGQE
                     EAEAIAASGVPCAELDADSVRSLEPGLVDEVIEGWLLEDGWRLNPSALTVSLALFPGV
                     EVRRHVTVRALLAGGGRVRGVVTDAGTIEADEVIVAAGPWTPALLRPLGVELPIVGAR
                     GWLVHLGCDDPPVGRLVERAGWHAVGGEEAMSRLTAKGLIEAVPEPDVGTLVQPNPDG
                     TVLVGGSRQRVVAPEPEDPSVPRELTRRAVDLVPPLGEARVLGSWWGIRPITPDGLPI
                     VARVMDGLVVATGHGSEGVILGGGTGRLVGSMLAGEEPPFDPAPFALR"
     gene            complement(6620..6970)
                     /locus_tag="VF097_06530"
     CDS             complement(6620..6970)
                     /locus_tag="VF097_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013873735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX6262488.1"
                     /translation="MDLMDRRRFHELVDEAMDGLPAWVRERLENVAVIVEDEPPEDDP
                     DLLGLYQGVPLTERGADYYGVLPDRIRLFRRVIEEEAESEEDVTRVVRETVIHEVAHF
                     FGISDERLGELDRD"
     gene            complement(6961..8427)
                     /locus_tag="VF097_06535"
     CDS             complement(6961..8427)
                     /locus_tag="VF097_06535"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEX6262489.1"
                     /translation="MSDPWTDRAPTPAYRAEPFAAQAWPSSSDGPERRGAFRRALLIV
                     LVITAAAGFSRFWTLGRPAEKVFDEVYYASDACLYAGHPFEACGLESEAERSWVHPPL
                     GKWLIALGIDAFGNDPFGWRFTAALAGTTSVALAGAFAFLLWGRPLWAGVAALLLATE
                     HLHVVQSRVAMLDIFLAFFVLLGFTLLLWDRVRQKRLDAVVAANEATPFLAPPGPRWI
                     RVAAGASFAAATASKWSGIFAWLGGLVLAVMWERGRRARAGVERPLLSALRQEWASLA
                     LAFIAAPLLVYLASWLRWLGDHGWSLPRLWSNHLSMANYHLGLEPFTESGEPIHPYMS
                     EAWTWLLLLRPVAYYYESMGDRAAEILGIGNPVLFWGALVFLPYLAFAWVRRRDWRAG
                     AILVPVFVLFAPWLFVTRPLFLFYMVPVTPFLALGATYALRDVSRLRGAGRPAPRLGA
                     AVAVAVAVGLFVFFWPVLIGDPISKRAWDARIWFSGWI"
     gene            8497..9324
                     /gene="rsmI"
                     /locus_tag="VF097_06540"
     CDS             8497..9324
                     /gene="rsmI"
                     /locus_tag="VF097_06540"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004930367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX6262490.1"
                     /translation="MAGTLILVGTPIGNLKDASERARETLARVHLIACEDTRRTGRLL
                     EALRIQRRARLVSYFEGNERKQTPYLIEQLERGSDVALVTDGGMPAFSDPGFRLVRAA
                     IAAGIEVDVVPGPAAAIAALVVSGLPTDRFAFEGFLPRKARLARLRELAGEPRTLLFF
                     ESPQRLRDMLRDAAEAFGPTRPAAVVRELTKLHQEIRRGTLGELADATPDEVKGEVTV
                     VVGGAEAAEPDLRALASQVNELVDQGLSRKEASGRVAEASGAGRRALYDASLQGEDA"
     gene            9347..>9645
                     /locus_tag="VF097_06545"
     CDS             9347..>9645
                     /locus_tag="VF097_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX6262491.1"
                     /translation="MPKDTFYVTTPIYYPNDVPHIGHAYTTVATDFVARYHRLRGERV
                     FFLTGTDEHGKKVPQAAEEHGLDPQAFVDQMEPRWREVWQRLDIAHDDYIRTTEPR"
BASE COUNT         1387 a         3354 c         3524 g         1380 t
ORIGIN      
        1 cacctccggc tcgtctccat cggtcgcggc ccaggcggcc agcgggctgc ccagcgccac
       61 cagaagtacg accaggacgg cgagccagcg ccgcgtgacg ccggccagag ccccgtgtcg
      121 ataatggtgc gtcatctcct caacctcctc tttcccccgg aagcagtaac gggtcccccg
      181 aacgacccag gggggccgtc gggattcgaa tcacctggaa ccttcggtac gtgctgtgcg
      241 ctcgggtccg tgcgggaatc ggccccggcg gggccttcgc gatccgcgcc ctcctgcatc
      301 ccgctttcct ccttgctgac gaggacgttc cgccaccgcg gagcgaagct cctacggcgg
      361 gtggcggggc gggcgccggt cggctcgtcc tgcttcgtcc ccctcgtggg ggacgcttcc
      421 cccatacggg ggataaggac acttatcagt gtgccagccg cgggaccgag tgagcaccgc
      481 ccatcagtgc caattgggga tggttagtta gggctacggt tctctgatgg gatgggtcag
      541 gagattgccc ggtcgatcag ctctgcctgc tcctgctgat gcacggtctg gctcccggtc
      601 gccgggctgg ggctctcggg tcgagccacg agccggggac ggacgtcgag gacccgctcc
      661 atcttgaggt gcacgaagcg cgcggcgccc atgttttcgg gctcctcctg ggcccagacg
      721 acttcggccc ccgggtagcg atcgagctgc tcgcggaggg cggccgcggg gaacgggtac
      781 acctgctcga cccggaccag cgcgacgtcg tcgacctccc gttcctcgcg gccgcgcagg
      841 aggtcgtaga agaacttccc ctgacacagc agcacgcgtc gggcctcgga ggggccggac
      901 gggtcgggca gaacctcccg gaagcgaccc tgcgtgaact cgtcggccgg gctgcgggcc
      961 gccggaagcc ggagcgggga cttcggcgtc agcacgataa gcggcttgcg ccggtcgcgg
     1021 agggcctgcc gccgcaggag gtggaagtac tgcgcgggag tggagggcac cgcgacctcc
     1081 atattgtcct tcgccgccag cgtgaggtag cgctccagac gggcggagga gtgctcgggc
     1141 ccctggccct cgaagccgtg gggcagcagc atcacgaggc cgctcgtctg cccccacttg
     1201 tcctcggccg aggcgatgaa ctggtcgatc acgacctggg ccccgtttgc gaagtctccg
     1261 aactgcgcct cccagaggac gagcgcgccc gggtttgaca ccgagtagcc gtactcgaag
     1321 cccagcgccg cgagctcgga gagcaggctg tccagcgcga cgaagcgccc gggcgctccc
     1381 tcgaggttcg cgagcggcag gtactcctgc ccggtctcct ggtcgacgag cacggagtga
     1441 cgctggctga aggtgccccg gcgcgtgtcc tggccggaga gccggaccca cacgccgtcc
     1501 cggatcaggg acccgaaggc gagggcctcg gcaagcgacc agtccacggc gtcctcggga
     1561 agggccttcc tccgctgatc gagccaccgc tggagcttct cgtggacgtg gaaggtctcg
     1621 ggaaccgtgg cgatcccctc gatcacctcg tcgagcaccg cacggtcgac gcccgtctcg
     1681 atcggaggtg ccggctccgc ctcgcgcgcc tcccgagccg aggggcgctg aggctgcggc
     1741 tcggccttcg tctcgtccag cgcctgctga agggtctccc ggtattcctc gagcgagcgc
     1801 tccgcctcct cgacggtgag gaggcctcgg ttgacgagct gctccatgta gcgcttgcgc
     1861 accgagcgaa ggcttcggat cttctcgtac atcagcggct gcgtgaacgc gggctcgtcg
     1921 ccctcgttgt gaccccaccg ccggtagcac cacatgtcga tcacgacgtc gcgatggaag
     1981 gtctcccgga aggcgaaggc cagcctggcc acccggacgc acgcctcggg atcgtcgccg
     2041 ttcacgtgga agatcggggc ctggaccatc ttcgcgacgt cggtggcgta ggtcgaggag
     2101 cggccgtagt ccgagccggt ggtgaacccg agctggttgt tgacgatgat gtggacggtc
     2161 ccgccggtcg agtaaccctg gagctgggag agagcgaagg tctcggccac gaccccctgc
     2221 ccggcgaagg cggcgtcccc gtggatgagg agtgggagca ccgggtcctc gtcgtcgcga
     2281 ccgagctgct cgagcttggc cctcgccatc ccctggagca ccgggtccac ggcctcgagg
     2341 tgggaggggt tggagaccac ggagaccacg agctggttcc cggcgcggga cgtgtgtttg
     2401 ccggccgccc cgacgtggta cttcacgtcg cccgacccct gcgaggatag ggggtcgatg
     2461 tctccctcga actgccggaa gatctgcgcg tacgacttcc cgatcacgtt cgccagcacg
     2521 ttgaggcgtc cgcggtgggc catcccgatc agcgtctcct cgatcgccga gtcggccgcc
     2581 aggtcgagga tcgcgtcgag catcgggatc atgctctcgg ccccctccag tccgtagcgg
     2641 cgatggccca cgtacttcgt gtggaggaac tgctcgaacg cctcggcctg gttcagcttc
     2701 gagaggatgt aggtgcggtc ctccttcgtg aggtcggcct gtgggccctc catccgctcc
     2761 tggatccacc gcttgggctc gacgtcctgc atgtgcatgt actcgacggt ggaggttcgg
     2821 cagtaggcgt ctcgcaggac ggtcaggatc tcgccgaggc tcatctcctc ggtgcccgcg
     2881 agcccgccgg tggggaagcg gcggtcgagg tcccagatcg tgaagccgaa gaacgcggga
     2941 tcgagctccg ggtgcatcgc gggcggctcg gactcgagcg ggtcgaggtt cgcgatgagg
     3001 tggccgcgca cccggtacat gttgatcagc tgggagacgc gggcctcctt ctcgtcggcg
     3061 ccgagcgtgc ccggccgagg attgacgtct cggcgccact cgacggggac gtagggcacc
     3121 cccatcgagg tgaagacccg atcgtagaag tcctctccgc ccagcaggag ccgctccacg
     3181 tccctgagga actcgcccga ctcagcgccc tggatcacgc ggtggtcgta ggtcgaggtg
     3241 agggtgagga ccttgcccac gcccagctcc gccagggtgt ccgggtcggc gcccgaatac
     3301 tcggcgggat ggccgatcgc ccccacgccg atgatcgcgg cctggcccgg catcagccgg
     3361 gggacggact ggacggtgcc gagcatcccg gggttcgtga tcgagacggt cgtgccggcg
     3421 aagtcgtcgg gcctgagctc gttccgctgg accttctcca ccatctcctc gtaggaccgc
     3481 cagaaggcgt cgaactcgag ggtctccgcc tccttgatgt tcgggacgag gagggtgcgg
     3541 ctcccgtcct ccttctggac gtcgactgcg aggcccaggt gcacgtgcgg gttgcggatc
     3601 gcgtgggggg tcccgtcgat ctcccggtag gcgacgttca tcgccggccg ctcggcgagg
     3661 gcccgaacca ccgcgaaccc gatcaggtgc gtgaacgaga ccttgccccc gcggttccgc
     3721 ccgaggtgcc ggttgagcat gcctcggttg acctcgagca gcttggccgg gaccgtgcgg
     3781 aaggacgtcg ccgtggggac gcccaggctc cggtccatgt tctcggcgat gatcttggcc
     3841 gcgccgcgga gcgggacggc ctcctcgggg acctcctcct tcggctcggg ctccggggga
     3901 gcctcctcac ggggggtggc gggtcggcgt tggccgtcgg tctcggggac gtagtcctcg
     3961 aagaactcct gccaggcctc gctgacggag ctcgggttct cctggtaccg gcggtacatc
     4021 tgctccacca accaggtgtt cgggccgaaa tcagaacgct tcacgcagcc tcgccttcgt
     4081 cgcttccttc tccatcctac ccaggaccgt caccccggcc ccctcggaat ctcaggcccg
     4141 cggcgggggc tgcgaggcca tctcggcgag ctgggcgggc catccggtgg cgagccacca
     4201 ggtcgcgcct gcctgccggt acgcggcggg gtcaggggga ggcccggcag cgacgacggc
     4261 gacgtctcgt ggcgcgcctg ccagatgctc gagctcgtcg gccgccgcgc ggaccccggg
     4321 gggcgatgga gggctgagat cgccgtccac ggggccggag aggaggaccc cgtcccatcg
     4381 cgcggcgcgc cgcagcggtg cccggtgggg gagggcgcag gcggcccaga tcgggatgcg
     4441 gggacgttgg acgggccggg ggaggaaggc cacgtcccgt gcggtgtagc cgggtccctc
     4501 gtggttcacg ggctcgccgg tccagagggc ggcgatcagg tggagggcct cgtccaggag
     4561 gggcgcgcgg tgctgggcgt cggcgggctc cccgaaggcg gcgaactcgg cgtcgggcgg
     4621 gttccccagc ccgacgccca gcaccgcgcg ccccccgctc agcacgtcga gcgtcgccgc
     4681 ctccctggcc acgacgtggg ggcgtcgccg ggccagcggc gtgaccgccg ttcccacccg
     4741 gatccgttcg gtgcggaccg cgatcgcccc gagcaggacc caagggtcga ggacctccat
     4801 cggcgcctcg gccggcgacc cgacgtggtc ccagaggaag aacccatccc agccgctcgc
     4861 ctctgcggct cgagcgagat cgaggaggtc gctcggggcc ccgaagttcg gcgcgtggat
     4921 cgcgaaccgc acgggctcag cccagcgccc gctcgaaggc gcggtaggtg gccacgtcga
     4981 acagcacgaa gcggacctcg tccacctggg tgtccgcctc ccggacggcc gcgatcgcca
     5041 ccggcgcggc ctcctccacc gggtacccgt agacgcccgt ggagatcgcc gggaagccca
     5101 cggtcctcgc cccgagctcg tcggcgaccc ggagcgactc ggtgtggcac gaggccagga
     5161 gctccgagcg gtcctcgctc ttcgcgtata ccggcccgac ggtgtgaatc acccaccggg
     5221 ccggcaggtt gcctgcgggc gtcgccactg cttgccccgt ggggaggccg ttcggatagg
     5281 tctccgcccg gatgcgcttg cacgcctcga agatcgccgg accgcccttc cgatggatcg
     5341 cgccgtccac ccctccgccg cccatcaggg aggagttggc cgcgttgacg atcgcgtcga
     5401 gctcctgctc ggtgaggtcc ccctgcgcta gcgtgatgcg cgccacgcat ccagtctagg
     5461 ctcggtgcga tgccggatgt gatcgtggtg ggaggagggg tgatcggcgc ggcctgcgcg
     5521 cgcgaggccg ctcgccgcgg catgtcggtg acgctcgtgg agcggtccga gctcgcggcc
     5581 ggtgcctcgg ggaggaacca cgggcttctt ctgacgccgc ccgaccccgc gttggtcccc
     5641 atgttccacg cctcgcgaga gctctacgtg gaggccgccg acggagcagc ggtgccgttc
     5701 gacctcgatc ccaagccgct cggtttcctc atcgtggcga ctgacgacca gggcgagcgt
     5761 gcggcggggc aggaggaggc cgaggcgatc gccgcgtcgg gcgtgccctg cgccgagctc
     5821 gacgcggata gcgtccggtc gctggagccc ggactggtgg acgaggtgat cgagggctgg
     5881 ctgctcgagg acgggtggcg gctcaatccc tcggcgctca cggtctcgct cgccctgttc
     5941 cccggggtgg aagtccgccg tcacgtgacg gtgcgtgccc tgctggccgg gggcgggcgc
     6001 gtgcgcgggg tcgtgaccga cgccgggacg atcgaggccg atgaggtgat cgtggccgcc
     6061 ggcccgtgga cgccggccct cctccgcccg ctcggcgtgg agctcccgat cgtgggggcg
     6121 cggggatggc tcgtccacct cgggtgcgac gacccgccgg tggggaggct cgtcgagcgc
     6181 gcgggctggc acgcggtggg gggcgaggag gcgatgtcga ggctcacggc gaagggtctg
     6241 atcgaggccg ttccggaacc cgacgtcggc acgctcgtcc agccgaaccc cgacgggacg
     6301 gtcctcgtgg gaggctcccg ccagcgcgtg gtggctcccg agccggagga cccctcggtc
     6361 ccccgcgagc tcacgcgccg agcggtggac ctcgtccctc cgctcggcga ggcgcgggtg
     6421 ctcgggtcct ggtggggcat ccgaccgatc acgcccgacg gcctccccat cgtggcgcgg
     6481 gtcatggacg gtctcgtcgt ggccacgggc cacgggtccg agggcgtgat cctgggaggc
     6541 gggacgggac ggctcgtggg ctcgatgctc gcgggcgagg agcccccctt cgatccggcg
     6601 cccttcgcgc tccgctgact cagtcccggt ccagctcgcc gagccgctcg tcggagatcc
     6661 cgaagaagtg cgcgacctcg tggatcaccg tctcccggac cacgcgcgtg acgtcctcct
     6721 cggactcggc ctcctcctcg atcactcggc ggaacagccg gatccggtcc ggtaggaccc
     6781 cgtagtagtc ggctccgcgc tccgtgagcg gtaccccctg gtagaggccg aggaggtcgg
     6841 ggtcgtcctc gggtggctcg tcctccacga tcacggccac gttctcgagc cgctcgcgga
     6901 cccaggccgg gagtccgtcc atggcctcgt ccacgagctc gtggaagcga cgccggtcca
     6961 tcagatccat cccgagaacc agatgcgagc gtcccaggct ctcttcgaga tggggtcgcc
     7021 gatcagcacg ggccagaaga agacgaagag gcccaccgcc acggcgaccg cgaccgctgc
     7081 gccgagccgg ggcgccgggc gcccggctcc gcggaggcgc gagacgtcac ggagggcgta
     7141 cgtggcgccc agcgccagga acggtgtgac cgggaccatg tagaagagga agagcggccg
     7201 ggtgacgaag agccacggcg cgaagaggac gaacaccggc accaggatgg cccctgcccg
     7261 ccagtcgcgg cggcggaccc aggcgaacgc caggtagggg aggaacacca aagctcccca
     7321 gaagaggacg gggttgccga tccccaggat ctcggcggca cgatcgccca tgctctcgta
     7381 gtagtaggcc acgggacgga gcaggaggag ccaggtccag gcctcggaca tgtaggggtg
     7441 gatcggctcg ccgctttcgg tgaacggctc cagcccgagg tgatagttcg ccatggagag
     7501 gtggttggac cagaggcgcg ggaggctcca gccgtggtcc ccgagccagc ggagccagct
     7561 cgcgaggtag acgaggagcg gcgccgcgat gaaggcgagg gcgagcgagg cccactcctg
     7621 gcggagagcg gagaggaggg ggcgctcgac gcccgctcga gccctgcggc cccgctccca
     7681 catgaccgcc aggaccagcc cgcccagcca ggcgaagatc cccgaccact tcgaagcggt
     7741 ggccgccgcg aacgaggcac cggcggccac ccggatccac cggggaccgg gcggcgcgag
     7801 gaagggcgtc gcctcgtttg cggcgaccac cgcgtccagg cgcttctgcc gcacgcggtc
     7861 ccagaggagc agggtgaacc cgagcaggac gaagaatgcg aggaagatgt cgagcatcgc
     7921 caccctcgac tgcacgacgt ggaggtgctc ggtggcgagc agcagcgccg ccacgccggc
     7981 ccagagcggg cgcccccaca gcaggaaggc gaaggcgccg gccagcgcca ccgacgtcgt
     8041 gccggccagg gccgccgtga accgccaccc gaacgggtcg ttcccaaacg cgtcgatccc
     8101 cagcgcgatc agccacttcc ccagcggggg gtggacccag gatcgctcgg cctcgctctc
     8161 caggccgcac gcctcgaacg ggtgccccgc gtataagcac gcgtcggacg cgtagtagac
     8221 ctcgtcgaag accttctcgg cgggccgccc cagggtccag aaccgggaga accccgcagc
     8281 ggcggtgatg acgaggacga tcagcagggc tcgacgaaag gccccgcggc gctccgggcc
     8341 gtccgaggag gacggccacg cttgggccgc gaagggctcg gcgcggtagg cgggggtggg
     8401 tgcgcggtcc gtccacgggt cgctcacgat gggagcatgc cacggccccg agccctcgtc
     8461 tcggacgcgg gcgcggtccg acctatcatc gaacggatgg cgggcacgct gatcctcgtg
     8521 gggacaccga tcgggaacct caaggacgcg tccgagcggg cccgggagac gctggcccgc
     8581 gtgcatctga tcgcctgcga ggacacgagg aggaccggcc ggctcctcga ggcgctccgg
     8641 atccagcgcc gagcccgtct cgtctcctac ttcgagggga acgagcgtaa gcagaccccg
     8701 tacctgatcg agcagctgga gcgggggagc gacgtggcgc tggtcaccga cggcggcatg
     8761 cccgcgttct cggaccccgg gttcaggctg gtgagggcgg cgatcgcggc cggcatcgag
     8821 gtcgacgtgg tgccggggcc ggcggccgcg atcgccgccc tcgtggtcag cggcctcccc
     8881 acggaccggt tcgccttcga gggcttcctc cccaggaagg cgcggctcgc ccgcctgagg
     8941 gagctggcgg gcgagccgcg caccctcctc ttcttcgagt cgccccagcg cctgcgagac
     9001 atgctccggg atgcggcgga ggccttcggc cccacccgac ccgcggccgt ggtccgcgag
     9061 ctcacgaagc ttcatcagga gatccggcgg ggaacgctcg gggagctggc cgatgccact
     9121 cccgacgagg tcaagggcga ggtgaccgtc gtcgtcgggg gcgcggaggc ggcggagccg
     9181 gacctgaggg cgctggcgtc ccaggtaaac gagctcgtgg atcaaggcct ctcgcggaag
     9241 gaggcctcgg ggcgcgtggc cgaggcatcg ggcgccggcc ggcgtgccct ctacgacgcc
     9301 tccctgcaag gcgaggacgc ctgaggctgt gggagaatcg cccgcgatgc cgaaggacac
     9361 cttctacgtc acgacgccca tctactaccc gaacgacgtc ccgcacatcg ggcacgccta
     9421 caccaccgtg gccaccgact tcgtggctcg ctatcaccgg ctccgggggg agcgggtctt
     9481 cttcctcacc ggcaccgacg agcacgggaa gaaggtgccc caggccgccg aggagcacgg
     9541 cctcgacccc caggccttcg tcgaccagat ggagccccgg tggcgcgagg tctggcagcg
     9601 cctcgacatc gcccacgacg actacatccg cacgaccgag ccccg
//
LOCUS       DASUTZ010000150         2251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_821311, whole genome shotgun sequence.
ACCESSION   DASUTZ010000150 DASUTZ010000000
VERSION     DASUTZ010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821311"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="VF097_06550"
     CDS             complement(<1..1059)
                     /locus_tag="VF097_06550"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX6262492.1"
                     /translation="MEPGGPRELLDRFWDEFLSLEPVFATLVGDERFDDRLPDPGPEG
                     LARRESVHRSTLEALKNVDRTGLGMEDRTALDMAESLARRDLAIVEHRLDRLWAVSHM
                     QGSYLCGPGLLPGQLGALQRADTPERVDGYLARLSAVPAYLEAIRGVMREAADAGQVA
                     PAVVVDRSIGLAERQLEAGAEASPAMQPVTEAGDRDRVAAVLADVVLPAYAGYLEALR
                     AYRPSARETLGLHALPRGEEMYAAEILGWTGLPLDPGELHALGRSHLDAIRAEGFAIA
                     SGLGFPGPAEAVAARADGEIARDAVLLVAREQVERGWEAAKGFFGRLPARNCEVRAVP
                     PDLEEDALDYYQGPTDDWS"
     gene            complement(1091..1528)
                     /locus_tag="VF097_06555"
     CDS             complement(1091..1528)
                     /locus_tag="VF097_06555"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX6262493.1"
                     /translation="MSQQSNQTIVQRYMEAFPMDFDALGQLRHPDYVEDWPQSGERIR
                     GHESYVRIHEQYPGGLPESEVRRIVGSEDRWVATPSFTLLRINGTGDTFTVEGMLTYP
                     TGSTTHLVAILRLRDGKVAEVTHYFADPFEAPAWRAEWVEPIP"
     gene            complement(1577..1948)
                     /locus_tag="VF097_06560"
     CDS             complement(1577..1948)
                     /locus_tag="VF097_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX6262494.1"
                     /translation="MPYPRWLAKVNKRLFNPWEIRKGKQPVVIHVGRSSGTEYRTPLD
                     AHPTKRGYVLVVRYGPVSDWVRNILAAGTAVLRVEGKEQRLGSPRLVSQREALDELAP
                     GLEPGKDFFKAEHYLLMDHRD"
     gene            complement(2051..>2251)
                     /locus_tag="VF097_06565"
     CDS             complement(2051..>2251)
                     /locus_tag="VF097_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020800094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX6262495.1"
                     /translation="REIVAGYWLLQVRSMDEAIEWAKRVPVEVAEHDYGQESEIEIRQ
                     VFDLEEFGEGPEVDRAGGLEEE"
BASE COUNT          318 a          854 c          727 g          352 t
ORIGIN      
        1 ggaccagtcg tcggtcggtc cctggtagta gtcgagggcg tcctcctcga ggtccggggg
       61 cacggctcgg acctcgcagt tccgagcggg cagccgaccg aagaacccct tcgcggcctc
      121 ccatccgcgc tccacctgct cgcgagccac cagcaggacg gcgtctcgcg cgatctcgcc
      181 gtcggctcgg gccgcgaccg cctcggctgg gcccgggaag ccgaggcccg aggcgatggc
      241 gaagccctcc gcgcggatcg cgtcgagatg ggagcgcccc agcgcgtgca gctctcccgg
      301 gtcgagcggg agtcccgtcc atccgaggat ctccgccgcg tacatctcct cccctcgggg
      361 aagcgcgtgg agcccgagcg tctccctggc cgaggggcgg tatgcgcgga gggcctccag
      421 gtagcccgcg tacgcgggca gcacgacgtc ggccaggacg gcggccaccc gatcgcggtc
      481 ccccgcctcc gtcacgggct gcatcgccgg cgaggcctcc gccccggcct cgagctgccg
      541 ctccgcgagc ccgatcgagc ggtccacgac cacggccggc gccacctgcc ccgcatcggc
      601 cgcctcccgc atgacccccc ggatcgcctc caagtaggcg gggacggcgg agagccgtgc
      661 caggtagccg tctacccgct cgggcgtgtc cgcgcgctgg agcgccccga gctgcccggg
      721 aagcaggccc ggcccgcaca ggtagctgcc ctgcatgtgg ctcacagccc agaggcggtc
      781 gagacggtgc tcgacgatcg ccaagtcccg gcgggccagg gactcggcca tgtcgagcgc
      841 cgtccgatcc tccatcccga gcccggtgcg gtcgacgttc ttcagggctt ccagcgtcga
      901 gcgatggacg gactcgcggc gggccaggcc ctccgggcct gggtccggaa ggcgatcgtc
      961 gaaccgctcg tcccccacca aggtggcgaa caccggctcc aggctcagga actcgtccca
     1021 aaagcgatcg agcagctcgc gcggccctcc gggttccatg ctcgtcacgc tcccgtgctg
     1081 cctgtgcctg ctaggggatg ggctcgaccc actccgcgcg ccaggccggc gcctcgaacg
     1141 ggtcggcgaa atagtgcgtg acctcggcca ccttcccatc ccggagccgc aggatcgcca
     1201 cgaggtgggt ggtgcttccg gtcgggtacg tcagcatccc ctcgacggtg aaggtgtccc
     1261 ccgtcccgtt gatccggagc agggtgaacg aaggagtcgc gacccatcgg tcctcgctcc
     1321 ccacgatccg gcgcacctcc gactcgggga ggccccccgg atactgctcg tggatcctga
     1381 cgtagctctc gtgaccgcgg atgcgctccc ccgactgcgg ccagtcctcg acgtagtcgg
     1441 gatggcgcag ctgcccgaga gcgtcgaagt ccatcgggaa cgcctccatg tagcgctgca
     1501 cgatcgtctg gttgctctgc tggctcatcc cccacccccg gtccgacgga tcggagcgag
     1561 catactccga ggcggatcag tcgcggtggt ccatcaggag gtagtgctcc gccttgaaga
     1621 agtccttccc gggctcgaga ccgggagcca gctcgtcgag ggcctccctt tgggacacca
     1681 gccgtggtga gccgagccgc tgctccttcc cctccactcg taggaccgcg gtcccggccg
     1741 ccaggatgtt ccggacccaa tcggagaccg ggccgtaccg tacgacgagc acgtatcccc
     1801 gcttggttgg atgggcatcg agcggcgtcc ggtactcggt ccccgacgat ctccccacat
     1861 ggatcacgac gggctgcttt cccttcctga tctcccaggg attgaagagg cgcttgttga
     1921 ccttcgccaa ccacctcggg tacggcatcg accacctccc gcgtggacgc tggtggactc
     1981 gatccaccgt tctccggacg tcgcgctgac agttctcgtc gctctggccc cggggagccc
     2041 ccgactccta ctactcttcc tcgaggccac ccgcccggtc gacctccggg ccctcaccga
     2101 actcttcgag atcgaagacc tggcggatct cgatctcgct ctcctgcccg tagtcgtgct
     2161 cggccacctc gaccgggaca cgcttggccc actcgatcgc ctcgtccatc gacctcacct
     2221 gcaggagcca gtagccggcg acgatctccc t
//
LOCUS       DASUTZ010000151         3255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1469998, whole genome shotgun sequence.
ACCESSION   DASUTZ010000151 DASUTZ010000000
VERSION     DASUTZ010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469998"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..962
                     /locus_tag="VF097_06570"
     CDS             141..962
                     /locus_tag="VF097_06570"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016435491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX6262496.1"
                     /translation="MPPEDLAPLVKGWPVEHMAAGVTSAESTLGVTGDPRWMTRIASV
                     AKLFVGLAALVALEEEALDLDEPAGPEGSTVRHLLAHASGLAFDEHRSIAPPGRRRIY
                     SNTGIEVFADHLAERTGMSFGEYMRLGVLDPLGMSGTELRGSPAHGLWSSVSELLLLG
                     RQLLRPTLVAAATLEDATRPHFPELAGVLPGLGRFDPNPWGLTFEIRGDKRPHWTGER
                     NSPATFGHFGGAGTFLWVDPAHGLAAAVLTGREFGDWALEAWPAFSDAALNRFAE"
     gene            1029..2198
                     /locus_tag="VF097_06575"
     CDS             1029..2198
                     /locus_tag="VF097_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX6262497.1"
                     /translation="MRRIVALACAGVVALSFGVAPPSGAEEQPGRLLSSFCGPGVSAP
                     CIESATRDGVPVTSADPIWSVFLVSYNEGKSRFVRLHVQKDGSFELGLDALDDVWVVT
                     VRTGSVVPRVVFGYAAESTIERFASPNRVTFTASPVRMTDNDECSPIWPWSCPHQASN
                     DFDGYLDVMATDYGAWADTAQRESMYGMDYTTNVGLTSIPPEIVHDPATGRERLLIRM
                     ANHHLYSDATLFEGFAHLRIPNLFLRRAYGVDHPPSLTGSGIDTAGTGSAATVTVMPE
                     PGGGAMLVDIEGMRFSRKRVRISRGVITPTKPRDLRARRSGKRVRLRFTEAKRRGSRI
                     TRYRASCVRGSSRLTGGARRSPVLVKNLTPGVKYTCRVRAISKAGPGRWSRKVRA"
     gene            2434..>3255
                     /locus_tag="VF097_06580"
     CDS             2434..>3255
                     /locus_tag="VF097_06580"
                     /inference="COORDINATES: protein motif:HMM:NF041045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor RsbA family regulatory
                     protein"
                     /protein_id="HEX6262498.1"
                     /translation="MRRSPRLSHPVLLYGDEEEFVAGTVPFVRRGIENDEVVFVAARP
                     DYLDTVRAELGADASAATWKDTHVWHPHHASRLRAVHELVSGAPRGSRLRLVGEPIWP
                     ERPEEVLEWQRFESALNAVLAPFPASLLCLYDAGSLDPGILETAGRTHPGAGYEEPET
                     FLRAHRGEPFPPPRSAEVVEHVRDPAWARGLALDRAIRAGVGGDAAVGLSIAVNEVLM
                     NALLHGGGEASLSMWEEGASFVCQVEDEGPGIADPLAGYRPPANRTGGRGLWIARQ"
BASE COUNT          455 a         1129 c         1185 g          486 t
ORIGIN      
        1 cgcatgaacc accatccgga catcgagaac cactacgacc gggtgcggat cgggctgcac
       61 acgtggagcg aggaagccgt caccgagaag gacctcgcgc tggcccgaga gatcgagcgg
      121 ctggccgggt aggctcgccg atgccccccg aagacctcgc ccccctcgtg aagggctggc
      181 ccgtggagca catggcggcg ggcgtcacga gcgctgagtc gacgctcggc gtgaccggcg
      241 accccaggtg gatgacgcgg atcgcgtcgg tggccaagct gttcgtgggg ctggccgcac
      301 tcgtggccct ggaagaggag gccctcgacc tcgacgagcc ggcgggcccg gagggctcca
      361 cggttcggca cctcctggcc cacgcctcgg ggctcgcctt cgacgagcat cgatcgatcg
      421 cgccgccggg gcgtcggcgg atctactcca acacggggat cgaggtgttc gccgaccacc
      481 tcgcggagcg gacggggatg tcgttcggag agtacatgcg cctcggtgtc ctggaccccc
      541 tcggcatgtc cgggacggag ctccggggct ccccggccca cgggttgtgg agctcggtct
      601 cggagctgct cctgctgggc cggcagctgc tacggcccac gctcgtggcg gcggccacgc
      661 tggaggacgc cacccggccc cacttccccg agctggccgg tgtccttccg ggcctgggcc
      721 gcttcgatcc caacccctgg gggctgacct tcgagatccg gggggacaag cggccccact
      781 ggacggggga gcggaactcc ccggcgacgt ttgggcactt cggaggggcg gggaccttcc
      841 tctgggtgga tcccgcccac ggcctggcgg ccgccgtgct cacgggccgc gagttcggcg
      901 actgggccct cgaggcgtgg ccggcgttct cggacgccgc gctcaaccga ttcgccgagt
      961 gagccgacct atacctgagg gtcggcccct ggtagcatcc ccgggcactt tgcgaggagg
     1021 gagcggagat gaggcggatc gtggccttgg catgcgcggg tgtggtggcg ctctcgttcg
     1081 gggttgcccc accgtccggg gccgaggagc agcccggcag gttgctatcg tccttctgcg
     1141 gaccgggcgt ttccgccccc tgcatcgagt cggctactcg tgacggggtc cccgtcacct
     1201 cagcggaccc catctggtcc gtcttcctcg tctcgtacaa cgaggggaag tcacggttcg
     1261 tccgcctgca tgtccagaag gacggcagct tcgagctggg actcgatgcg ctggacgacg
     1321 tctgggtggt cacggtgcgg accgggtcgg tcgttcctcg tgtggtgttc ggctacgccg
     1381 ctgagtccac gatcgagcgc ttcgcgtccc cgaatcgcgt gacgttcacc gcctctccgg
     1441 tgcggatgac cgacaacgac gagtgcagcc cgatctggcc gtggtcctgc ccgcaccagg
     1501 cgtcgaacga cttcgacggc tacctcgatg tgatggccac cgactacgga gcgtgggcgg
     1561 acaccgccca gcgtgaatcg atgtacggga tggactacac gacgaacgtc gggctgacga
     1621 gcatcccgcc cgagatcgtc cacgaccccg ccaccggacg cgagcgcctg ctgatccgga
     1681 tggccaacca tcacctgtac tcggacgcaa ccctcttcga ggggttcgcg cacctccgca
     1741 tccccaacct gttccttcgg cgcgcctacg gtgtcgacca cccgccgtcg ctcaccggct
     1801 cgggcatcga taccgcgggc acgggatcgg cggcgacggt gacggtgatg ccggaaccgg
     1861 gcggtggcgc catgctcgtc gacatcgagg ggatgcggtt ctctcgcaag agggttcgca
     1921 tctcgcgcgg cgtgatcacg cccaccaagc cgagggatct ccgggcccga cgcagcggga
     1981 agcgggtgag gctgcggttc accgaggcca agcgtcgagg ctccaggatc acccgatacc
     2041 gcgcctcgtg cgtgcgtggc tcctcaaggc tcacgggtgg ggccaggcgc tctcccgtgc
     2101 tggtgaagaa cctgacgccc ggggtgaagt acacgtgccg ggtgcgggcg atctccaagg
     2161 ccggtcccgg ccgatggtcg aggaaggtgc gggcctagcg agcccggtgg atctcgcccg
     2221 ccgctcggat ccccgactcc agggcgcccg ggagccgcat ccccccgagc tcggcgtaga
     2281 ggccgggagt ggaccaacga cgtgtgtgga aggctcgggt cgagggtagg ggcaacccca
     2341 tgagcgatcc cgccgctcac gagcgggccc cgaggccgag aggagacgcc ccgatgatcc
     2401 atcggctgag cccgtccatc gagtgggtcg gggatgcgac gtagcccccg gctctcgcac
     2461 cccgtgttgc tctatgggga cgaggaggag ttcgtcgcgg ggacggtccc cttcgtccgc
     2521 cgggggatcg agaacgacga ggtggtcttc gtggcggccc gacccgacta cctcgacacc
     2581 gtccgggccg agctgggcgc ggacgcttcg gcggccacct ggaaggacac gcacgtctgg
     2641 cacccgcacc atgcctcgcg gcttcgcgcc gttcacgagc tcgtcagcgg cgccccgagg
     2701 ggctccaggc tccgcctggt gggggagccc atctggcccg agcggccgga ggaggtcctc
     2761 gagtggcagc gcttcgagtc ggcgctgaac gcggtcctcg cgcccttccc cgcctcgctc
     2821 ctatgcctct acgacgcggg ttcgctcgac ccggggatcc tcgagacggc cgggcggacg
     2881 catcccgggg ccggctacga ggagcccgag acgttcctcc gcgctcaccg aggggagccg
     2941 ttccccccac cgaggtcagc cgaggtcgtc gagcacgtgc gcgacccggc ttgggcacgg
     3001 ggcctggccc tggaccgcgc catccgggcc ggggtgggcg gggacgccgc ggtcgggctg
     3061 agcatcgcgg tcaacgaggt cctgatgaac gcgctcctcc acggcggcgg ggaggccagc
     3121 ctctcgatgt gggaggaggg ggcctccttc gtatgtcagg tcgaggacga ggggccgggg
     3181 atcgccgatc ccctcgccgg ctaccggccg ccggcgaacc ggacgggggg ccggggcctc
     3241 tggatcgccc ggcag
//
LOCUS       DASUTZ010000152        12638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_563549, whole genome shotgun sequence.
ACCESSION   DASUTZ010000152 DASUTZ010000000
VERSION     DASUTZ010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12638
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563549"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="VF097_06585"
     CDS             complement(<1..681)
                     /locus_tag="VF097_06585"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6262499.1"
                     /translation="MTGPRIAVIGLDCATPLLLFDRLRSETPNIQALLGRSMHGELES
                     ITPPITVPAWACSMTGKTPGQLGIYGFRNRKDTTYDGLSIATSLAVKEPAVWDQLGEA
                     GMRSLLIGVPPAYPIKPVEGWRVSCFLTPPSADQYTHPADLKAEVEEVVGDYIFDIPN
                     FRRQGLDFVLSQSHEMTKKRFALARHMVRTKPWDFFMMVEMGTDRLHHVFWQYCDPDH
                     PKYEPGNPY"
     gene            826..1272
                     /locus_tag="VF097_06590"
     CDS             826..1272
                     /locus_tag="VF097_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262500.1"
                     /translation="MRLLRGALYAHALAWIVLGALLAIWPGVLQSWLGQPPYVDRAWV
                     RLVGVQAVGLALFMVLVGHRLEELWWWSWGFVLTTGAVAAVLTLNAAFGLPQGADAVL
                     WWVLAIGHWALGSATLVGMSRAARERPTDRYAPAPARDPHPRGRRR"
     gene            complement(1283..1474)
                     /locus_tag="VF097_06595"
     CDS             complement(1283..1474)
                     /locus_tag="VF097_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262501.1"
                     /translation="METFVVRVWPHEEADGLHGVVRHVRSGREVRFSTALQLADLLSR
                     GFPSEAPEPTGPPEEPAAR"
     gene            complement(1518..4760)
                     /locus_tag="VF097_06600"
     CDS             complement(1518..4760)
                     /locus_tag="VF097_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX6262502.1"
                     /translation="MKTLPPARLGPLVARPRLERRLEDALSCRLTVVLAGAGFGKTTL
                     LCAWAAAARSAWYTLDATDRSLPVLVRGLADALRLRVPGLYAEVAMALEGAAGPNADD
                     PSRAQALASLVAEGLARELDEDLLLVLDDVHEAEGSPASVRLIDELSRQAPQPLHVVL
                     ASRTEVPFQIERLRGRGQVLDLPVAALAFTEEEVAELLRAVLGEEPGDLAGSLHRLTE
                     GWPAAVRLGAEVAASADPEHRTGALEALSRPGSPVFDYFAREVLGAEEPHVLGFLRAA
                     ALLDAFSPELCTAIGVEDAERTIADLSSRGLFFEPRGEGWFELSPLIRGVVERSDPGG
                     GEAAALLTSAAKWLLEAGRIEDALGALLRADDLKGVAAVLEEHGEGLIAGGSVEAVIR
                     SIEPLPADLRSTALDRLEGEARQIRGDWDRALACFLRAAPKEGPLDAGLAWRMGLIHH
                     LRGDLGAALATYERGRVDREDRDVALLLAWSAAAHWLRGDLERSRETAGRARDAAAGV
                     GDERAEAAAFTTLAMVAALEGDRRANDAYYLRALDAAERARDVLQIVRIRTNRASRFS
                     EEGDHEDALRELDLAVGLAELTGFANFHALGVHNRGQARLGLGRLEEARTDFESALAL
                     YQRMGSRMVAYPLAGLADVSRTRGDLAQARAAYEEAVAVAEEAGDLQGLVPAMAGLAR
                     VLAAEDPEAARSTAERAVELGRGMGYVQAQLALGWVALAGEDPDAARGAAKAASEAAR
                     ERRDRPGLAEALTLAAMTDPGQPWAPTALDEAAGIWREVGNPLEEAVVGIVAPALGGR
                     TEAGPSAERARRRLRAYGVRLPAAAQPAGPLHLLSGDEPSLVIQALGGFRVLRGGRAL
                     PASEWQSRKARDLLKLLVARRGRPAPRELLMEVLWPEEDPDKVANRLSVALTTVRTVL
                     DPERGRPQDHFVHADREAVALEIDRLDVDVERFLADGTTALDLVQGGSTEEALPLLRE
                     AEAAYAGDFLEENLYDDWAVALREEARATYLSVCRELARLAVAEGDHDAAIRYLLRII
                     ERDPFEERAHLGLVTVLTRAGRHGEARRAYRIYRSRMEELEVEPAPFPSA"
     gene            complement(4873..6495)
                     /gene="groL"
                     /locus_tag="VF097_06605"
     CDS             complement(4873..6495)
                     /gene="groL"
                     /locus_tag="VF097_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018154970.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX6262503.1"
                     /translation="MPGKELKFHEDARRALEAGVDKLADTVSITLGPKGRNVVLDKKW
                     GAPTVTNDGVTIAKEVELEDPWENMGAQLAKEVATKTNDVAGDGTTTATVLARAMVKQ
                     GMKNVAAGANPMALKRGIEQGVAAAVEAIEKQSREVEGKDEIAHVAAISAADPKIGEI
                     IAEALEKVGKDGVVTVEESQTFGMQLEFTEGMQFDKGYISPYFITDQDRMEAVLDEAY
                     ILLVGKKISSVQELLPVLEKVSQTGKPLVIIAEDVEGEALATLVVNKIRGTFKSSAVK
                     APGFGDRRKAMLEDMAILTGGQVISEEVGLKLESVSLDIMGTARRVVITKDDTTIVEG
                     AGEEPAIKGRIEQIRRQIDETDSDWDREKLQERLAKLSGGVALIKVGAATEVELKEKK
                     HRIEDALSATRAAVEEGIVPGGGVALIRAEEALDGLNLEGDEATGAGIVRTALSEPPR
                     LIAANAGFEGSVIVERLRGEKAATGFNAATGEWVDMIKAGILDPAKVTRSALQNAASI
                     AALVLTTESAVVEMPEEEDEAAAAAAGGGHMH"
     gene            complement(6506..6802)
                     /gene="groES"
                     /locus_tag="VF097_06610"
     CDS             complement(6506..6802)
                     /gene="groES"
                     /locus_tag="VF097_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX6262504.1"
                     /translation="MAKFSLEPLDDRVVVKPGEEEETTASGIVIPDTAKEKPQEGEVV
                     AVGPGRFEDGSRVPMDVKVGDTVLYSKYGGTEVKIEGEEHLVLSARDILAIVKK"
     gene            7068..7685
                     /locus_tag="VF097_06615"
     CDS             7068..7685
                     /locus_tag="VF097_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262505.1"
                     /translation="MVEAGSSRRLFEREVRMHKSKLRVGALIVAATVIGGAMIIPAGA
                     ADGVSRAFVRRTVKKFINRDNNKNEGIFASFNNGPGIVPTGIATIASRSVPKGNYAIF
                     GKVTIRDAGDLDTECLIQAGADFDHGWARVEANSSLAASGVTLNLEVVHSFGAGGGPI
                     LLRCDDHGDADDWEDAKIIAIRQPKLRNTVSPAFAGTSAQDPDDA"
     gene            complement(7744..8700)
                     /locus_tag="VF097_06620"
     CDS             complement(7744..8700)
                     /locus_tag="VF097_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6262506.1"
                     /translation="MSDRPGTVLVTGAAGFVGSHLSDRLLAQGYRVIGIDDLSVGRIA
                     NLAEARGYGKDFQFYNLDIRGEAIPAVFEKHQPELVMHLAAQSGVRPSLEDPRKDADI
                     NVLGTLNLLEAAARAGTRKIVYAASGGTIYGEPRRLPAKETATSGSHPQSPYGISKRV
                     VHDYLLYFQRYEGLDFTSLALANVYGPRQDPHGEAGVVAIFGRRMLAGERPQINGDGN
                     QTRDYVYVEDVVDAFARAVDAGSGKLINIGTGLETSVNHLFRLLAELTGYAGEPSFGP
                     LPKGDLRRSSLDPSLAERDLGWKPWTQLEEGLRQTVEWLKAG"
     gene            complement(8697..9701)
                     /gene="tsaD"
                     /locus_tag="VF097_06625"
     CDS             complement(8697..9701)
                     /gene="tsaD"
                     /locus_tag="VF097_06625"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX6262507.1"
                     /translation="MRVLGLETSCDETAVAVVEDGVKIHSNLLASQHDVHARFGGVVP
                     ELASRAHVERLGPLMDRALDEAGIGWRDVEAVAVTRGPGLVGALLVGLATAKAAALAL
                     KVPFLGVNHLEGHVYANFLEHGPPEPPYVALIVSGGHTLLVHMPEEHRYEVVGQTLDD
                     AAGEAFDKIARFLGLGFPGGPALDKLAREGDPEAIHFPRAMAGSGDYDFSLSGLKTAV
                     IRYVRREQEAGREISTGDLAASFQEAIVDVQVQKTFAVAEERGVETVLLGGGVVANTR
                     LRERMSEEAGRRGVRLHYPPPELCTDNAAMIASAAYFRIRRGARDGWDVAADPGLALA
                     "
     gene            complement(9698..10192)
                     /gene="rimI"
                     /locus_tag="VF097_06630"
     CDS             complement(9698..10192)
                     /gene="rimI"
                     /locus_tag="VF097_06630"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX6262508.1"
                     /translation="MRRRHLKGVLAIERQVYPRPWSSNLFLAELRNHKDRAYYVALID
                     REVVGYGGLLSYGDEAHVTTIAVHPEHQRHKIGTRLLIELIDQAGRMEAVAVSLEVRV
                     TNHGAQRMYGRFGFRPVGVRKNYYQEIGEDALVMWTDDIRTEEYGRLLEGLRASLPEG
                     LRRP"
     gene            complement(10209..10913)
                     /gene="tsaB"
                     /locus_tag="VF097_06635"
     CDS             complement(10209..10913)
                     /gene="tsaB"
                     /locus_tag="VF097_06635"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX6262509.1"
                     /translation="MLVLALETSTPQTSVAIGSEQGVVASAVVSWGRGHSEVTVPAIE
                     RLLAWSEIDLDQVGGVAVGIGPGLFTGLRVGISTAKGLAQVLGLPMVGLGSLDVLAYS
                     MRYARERICAVLDAKRSEVFWSFYRSVPGGVTRETEPSVGPPERLAAELEASPDETLL
                     VGGGALMHRRVLERDASGVDFASTANAFPLAVWLLELAVPRFHREETSSPHEILPLYL
                     RKSDAEINWDRRARSA"
     gene            complement(10913..11404)
                     /gene="tsaE"
                     /locus_tag="VF097_06640"
     CDS             complement(10913..11404)
                     /gene="tsaE"
                     /locus_tag="VF097_06640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX6262510.1"
                     /translation="MGSEIRLRTRSADETREVGAAIARALVPGDVVSLTGDLGAGKTT
                     LVQGSARELGVTDPVASPTFVLVREYRGSLPIYHVDVYRLERIQEVLDLGFEDLLDPH
                     GVVFIEWGGAIEGLLPDSHLEVELHALEGDEEREVAVRARGPGWAARWVDLSGSLGSW
                     RAA"
     gene            complement(11404..12000)
                     /locus_tag="VF097_06645"
     CDS             complement(11404..12000)
                     /locus_tag="VF097_06645"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX6262511.1"
                     /translation="MSQLEEIARTASTCTRCRLSEGRTQVVFGDGNPNAGIMFIGEGP
                     GFHEDRQGVPFVGAAGQLLNRLLEEVGLSRGDVYIANVVKCRPPGNRDPMPDEIEECS
                     PYLMQQVDLVDPRVIVTLGNFATRVILQRQVSISRVRGQREAWRGRTVIPTFHPAAAL
                     HGGGEGGPQMRALREDFAAIKAAVEEGAPAVEEQLGLF"
     gene            complement(11997..>12638)
                     /locus_tag="VF097_06650"
     CDS             complement(11997..>12638)
                     /locus_tag="VF097_06650"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX6262512.1"
                     /translation="FDGGVARVPIVPAAVIFDLDAGDPAVRPGPDQGYAACEAASEEV
                     PEGSVGAGTGATVGKLPGREHAVKGGVGTSSAAEDGVTVGALVAVNAVGAVVDEEGAS
                     IAGRLLEEGEEPSPPFGNTTLAVVATDARLSRERAHLLAIAAHDGLASAIRPAHTMWD
                     GDTVFTLATGAVEASQSLLERLAEDALADAVRRAVRLARGLAGIPPAGEEGGR"
BASE COUNT         1721 a         4757 c         4295 g         1865 t
ORIGIN      
        1 gtaggggttg cccggctcgt acttcgggtg gtccggatcg cagtactgcc agaagacgtg
       61 gtggagccgg tcggtcccca tctcgaccat catgaagaag tcccagggct tggtcctcac
      121 catgtggcgg gccagggcga accgcttctt cgtcatctcg tgagactggg agagcacgaa
      181 gtcgaggccc tgccgccgga agttggggat gtcgaagatg tagtccccca ccacctcctc
      241 gacctcggcc ttgaggtcgg cgggatgcgt gtactgatcg gccgatggag gtgtgaggaa
      301 gcacgagacg cgccacccct cgaccggctt gatggggtag gcgggcggga cgccgatcag
      361 cagggagcgc atcccggcct cgcccagctg gtcccacacc gcgggctcct tcacggccag
      421 cgaggtggcg atggagaggc cgtcgtaggt ggtgtccttg cggttccgga agccgtagat
      481 cccgagctga cccggggtct tccccgtcat ggagcaggcc cacgccggca cggtgatcgg
      541 gggcgtgatg ctctccagct cgccgtgcat cgaccgcccc aggagagcct ggatgttcgg
      601 ggtctccgac cgcagccggt cgaacagcag cagcggcgtg gcgcagtcga gcccgatcac
      661 cgcgatcctc ggtccggtca tgcctcagcg tcctttcggg gtggagagcc tttcagggcg
      721 gcgcgagcag gccggcgccg accgctcatt ctagccgact cccgacctcg ctccccagga
      781 acagggtggg tcgcgccggg gacgtcggtc ggagaggata ctgggatgcg gctgctccgt
      841 ggggcgctct acgcgcacgc gctcgcctgg atcgtgctgg gcgcgctcct cgcgatctgg
      901 cccggcgtgc tgcagtcctg gctgggccag ccgccctacg tcgaccgtgc ctgggtccgg
      961 ctcgtcgggg tccaggccgt cgggctggcg cttttcatgg tcctcgtggg gcaccgtctc
     1021 gaggagctct ggtggtggtc gtggggcttc gtgcttacga ccggggccgt ggccgcggtg
     1081 ctcaccctga acgcggcctt cgggctgccg cagggagccg acgcggtgct gtggtgggtg
     1141 ctggcgatcg gccactgggc cctgggatcc gccacgctgg tcgggatgtc gcgggccgcc
     1201 cgagagcgcc ccaccgaccg ctacgcgccc gcgcccgccc gcgacccgca cccgcgggga
     1261 cggcggcgct gagggggatc gttcatcgtg ccgccggctc ctcgggcggc ccggtaggct
     1321 ccggggcctc ggaaggaaat ccccgactca ggaggtcggc gagctgcagc gcggtcgaga
     1381 accggacctc ccggccgctc cggacgtgcc gcacgacacc gtgcagccca tcggcctcct
     1441 cgtgcggcca cacgcgaacc acgaacgtct ccacgcgcgg atgctgacac gggatccttc
     1501 gggttccctg aaggtgccta cgccgagggg aagggcgccg gctccacctc cagctcctcc
     1561 atcctgctcc ggtagatgcg gtaggccctc ctggcctccc cgtgtctccc cgccctcgtc
     1621 aggacggtca cgaggccgag gtgcgcccgc tcctcgaacg gatcccgctc gatgatgcgg
     1681 aggaggtacc ggatcgccgc gtcgtgatcg ccctcggcga ccgccaggcg cgccagctcc
     1741 cggcacacgc tcaggtaggt ggccctcgcc tcctcccgca gcgccacggc ccagtcgtcg
     1801 tagaggttct cctcgaggaa gtcccccgcg taggcggcct cggcctcgcg caggagaggg
     1861 agggcctcct cggtggaccc tccctggacg aggtcgaggg cagtcgtccc gtcggccagg
     1921 aagcgctcga cgtcgacgtc caggcggtcg atctcgagcg ccacggcctc gcggtcggcg
     1981 tgtacgaagt ggtcctgggg tctcccccgc tccggatcga gcacggtccg aacggtggtg
     2041 agggccaccg agaggcggtt ggccaccttg tcgggatcct cttcgggcca caggacctcc
     2101 atgaggagct cgcggggggc gggccgtccc cgcctcgcca ccaggagctt caggaggtcc
     2161 ctggccttcc tcgactgcca ctcggaggcg gggagggccc gccccccgcg cagcacgcgg
     2221 aaccctccca gcgcctggat cacgagcgat ggctcgtccc ccgacagcag gtggagcggt
     2281 ccagccggct gggcggccgc gggcaagcgc acgccgtagg ccctgaggcg ccgcctggcc
     2341 ctctccgcgg acgggcccgc ctcggtccgg ccaccgaggg ccggcgcgac gatccccacg
     2401 accgcctctt ccagcgggtt tcccacctcc cgccagatcc ccgcggcctc gtccagcgcc
     2461 gtcggcgccc aaggctggcc cggatccgtc atcgcggcca gggtgagggc ctcggcgagc
     2521 ccggggcggt cgcgtcgctc acgggcggcc tcggacgccg ccttggccgc cccccgcgcc
     2581 gcgtccgggt cctcgcccgc cagcgccacc cagcccagcg cgagctgcgc ctgcacgtag
     2641 cccatgcccc gccccagctc cacggcccgc tcggccgtcg atcgggccgc ctccggatcc
     2701 tccgcggcga gcacgcgggc cagcccggcc atcgccggta cgaggccctg gagatccccc
     2761 gcctcctcgg ccacggccac cgcctcctcg taggccgccc gggcctgggc caggtcgcct
     2821 cgggtccggg agacgtcggc caggcccgca agcgggtagg ccaccatccg agagcccatc
     2881 cgctggtaga gggcgagggc cgactcgaag tccgtccgtg cctcctccag ccgacccagg
     2941 ccgagccggg cctggccccg gttgtgcacg ccgagggcat ggaagttggc gaagccggtc
     3001 agctcggcca gccccacggc caggtcgagc tcgcggaggg cgtcctcgtg gtcaccctcc
     3061 tcgctgaagc gcgaggctcg attcgttcgg atcctgacga tctggaggac gtctcgggct
     3121 cgctcggcag cgtccagcgc ccggaggtag taggcgtcgt tggcccggcg gtccccttcc
     3181 agcgcggcca ccatggccag cgtggtgaag gccgcggcct cggccctctc gtcccccacc
     3241 cccgccgccg cgtcgcgagc ccgcccggcc gtctcgcggg accgctccag gtccccccgc
     3301 agccagtggg cggccgcgga ccaagccagg aggagggcga cgtcccgatc ctcgcgatcc
     3361 acccgtcccc gttcgtacgt ggcgagcgcg gccccgaggt ctccccggag gtgatggatc
     3421 agacccatcc gccaggccag cccggcgtcg agaggcccct ccttcggcgc cgcgcggagg
     3481 aagcaggcca gggcgcgatc ccagtccccg cggatctgcc gcgcctcgcc ctcgaggcga
     3541 tccagggcgg tggagcgcag atcggcgggg agcggctcga tcgatcggat cacggcctcg
     3601 acggaccctc ccgcgatcag cccctcgccg tgctcctcca ggaccgcggc cacccccttg
     3661 agatcgtccg cgcgcaggag ggctccgagc gcgtcctcga tcctccccgc ctccagcagc
     3721 cacttggcgg ccgaagtcag cagcgcggcg gcctcccctc cccccgggtc ggagcgctcg
     3781 accacccccc ggatcagcgg cgacagctcg aaccagccct ccccccgcgg ctcgaagaag
     3841 aggccccggg aggacaggtc ggcgatcgtc cgctccgcgt cctccacccc gatcgccgtg
     3901 cagagctcgg ggctgaacgc atcgaggagg gcggccgccc tgaggaaccc caggacgtga
     3961 ggctcctccg cgcccagcac ctcgcgggcg aagtagtcga agacggggct gcccgggcgg
     4021 ctgagggcct ccagggcgcc cgtccgatgc tcgggatcgg cggaggcggc cacctccgcc
     4081 ccgagccgga cggccgccgg ccacccctcg gtgagccggt ggagcgagcc ggccaggtcg
     4141 ccgggctcct ctcccaggac ggcccggagc agctcggcga cctcctcctc ggtgaaggcg
     4201 agcgcggcca cgggcaggtc gaggacctgc ccgcggccgc gcagacgctc gatctggaac
     4261 ggcacctcgg tcctcgatgc cagcaccacg tggagcggct ggggggcctg ccgcgagagc
     4321 tcgtcgatca gcctgaccga ggccggcgac ccctcggcct cgtggacgtc atccagcacg
     4381 aggaggaggt cctcgtccag ctcccgggcc agcccctccg ccacgaggga cgccagcgcc
     4441 tgggcccgcg acgggtcgtc cgcgttggga ccggcggcgc cctccagagc catcgccacc
     4501 tcggcgtaca gcccggggac cctcagccgg agcgcgtcgg ccaggcccct gaccagcacc
     4561 ggcagggacc gatccgtggc gtcgagcgtg taccaggccg atctcgccgc ggcggcccag
     4621 gcgcagagca gggtggtctt gccgaagccg gcccccgcca ggaccacggt caggcggcac
     4681 gagagcgcat cctccaggcg gcgctccagc cgcgggcggg ccacgagggg accgagccgg
     4741 gcgggcggga gggtcttcac ggagggcgtt gatctcacgg gcctcgtccc tgcttgttcc
     4801 ggtgggccca tcctacggga acgcgagagg gaccgggcgt ccgcccggtc cctctcgcga
     4861 acgaaccgcc gttcagtgca tgtggccgcc gcccgcggcg gccgccgcgg cctcgtcctc
     4921 ctcctcgggc atctccacca cggcgctctc ggtggtgagc accagcgccg cgatcgacgc
     4981 cgcgttctgg agcgcggagc gcgtgacctt ggccggatcc aggatcccgg ccttgatcat
     5041 gtcgacccac tcccccgtgg cggcgttgaa gcccgtggcg gccttctcgc cccgcagccg
     5101 ctcgacgatc accgagccct cgaagccggc gttcgcggcg atcagacgcg gcggctccga
     5161 gagcgccgtg cgcacgatcc cggcgcccgt cgcctcgtcg ccctcgaggt tcagcccgtc
     5221 cagcgcctcc tcggctcgga tgagagccac gccgcctccg gggacgatcc cctcctcgac
     5281 ggccgcccgg gtcgcggaga gcgcatcctc gatgcggtgc ttcttctcct tcagctcgac
     5341 ctcggtggcg gccccgacct tgatcagcgc cacgcctccg gagagcttgg ccagccgctc
     5401 ctggagcttc tcgcggtccc aatcggagtc cgtctcgtcg atctgacggc ggatctgctc
     5461 gatccggccc ttgatcgcgg gctcctcgcc ggcgccctcg acgatcgtag tgtcgtcctt
     5521 ggtgatgacg accctgcggg cggtccccat gatgtcgagg ctcacgctct ccagcttcag
     5581 gccgacctcc tcggagatca cctggccccc ggtcaggatc gccatgtcct cgagcatcgc
     5641 cttgcgacgg tctccgaacc ctggcgcctt caccgctgag gacttgaagg tgccccggat
     5701 cttgttgaca accagcgtgg cgagggcctc gccctcgacg tcctcggcga taatcaccag
     5761 gggcttcccg gtctgggaga ccttctccag caccggcagc agctcctgca cgctcgagat
     5821 cttcttcccc acgaggagga tgtaggcctc gtcgaggaca gcctccatcc ggtcctggtc
     5881 ggtgatgaag tacggcgaga tgtagccctt gtcgaactgc atcccctcgg tgaactcgag
     5941 ctgcatgccg aaggtctggg actcctcgac cgtcaccacg ccgtccttcc ccaccttctc
     6001 cagggcctcg gcgatgatct cgccgatctt ggggtcggcc gctgagatcg cggcgacgtg
     6061 cgcgatctcg tccttgccct cgacctcgcg cgactgcttc tcgatcgcct cgacggctgc
     6121 cgccacgccc tgctcgatgc cgcgcttgag cgccatcggg ttggcgccgg cggcgacgtt
     6181 cttcatcccc tgcttcacca tggcccgcgc cagcacggtc gcggtcgtgg tgccgtcacc
     6241 ggcgacgtcg ttcgtcttgg tggcgacctc cttggccagc tgggccccca tgttctccca
     6301 cgggtcctcc agctcgacct ccttggcgat cgtcaccccg tcgttcgtga cggtgggggc
     6361 gccccacttc ttgtcgagga cgacgttgcg gcccttcggc ccgagggtga tcgagaccgt
     6421 gtcggcaagc ttgtcgaccc ccgcctcgag ggccctccgg gcgtcctcgt ggaacttcag
     6481 ctctttcccc ggcatgaacc ctcccttact tcttgacgat ggccaggatg tcgcgggcgg
     6541 agagcacgag gtgctcctcg ccctcgatct tcacctcggt gcccccgtac ttcgagtaca
     6601 ggaccgtgtc gccgaccttg acgtccatcg ggacgcggga cccgtcctcg aagcggccgg
     6661 gccccaccgc gacgacctcg ccctcctggg gcttctcctt cgcggtgtcg gggatcacga
     6721 tgccggaggc ggtggtctcc tcctcctcgc ccggcttcac gaccacgcgg tcgtcgagcg
     6781 gctccaagct gaacttggcc atacgttccc tccttgggtc ctcgttagca ctcgatactg
     6841 gcgagtgcca agtctactga ccgcgtccgc gagcggcaac cccgggatcg ggggcgccag
     6901 gcgcctacct caccgtcctc gtcggccgat cggaggtcgt gcgagcgaac cattgacatg
     6961 catcctcggg tgtggtctct ttcctccggc gttcatcgag aacgtcgtcg gcagtccatg
     7021 aaggcatgct gcaggacgag cgtcaatggg gcccgcgatg agcatcgttg gtcgaggcgg
     7081 gatcgtcccg ccgcctgttc gagagggagg ttaggatgca caaatcgaag ttgcgcgtgg
     7141 gcgcgctgat cgtggccgcc acggtcatcg gaggcgccat gatcatcccc gcgggcgcgg
     7201 ccgatggggt ttcccgggcg tttgtccgcc gtaccgtgaa gaagttcatc aaccgggaca
     7261 acaacaagaa cgaggggatc ttcgcctcgt tcaacaatgg cccgggcatt gtcccgacgg
     7321 ggatcgccac gatcgcttcg cgctctgttc ccaagggcaa ctatgcgatc ttcggaaagg
     7381 tgacgatcag ggacgctggg gatctcgaca ctgagtgcct catccaggcg ggagccgact
     7441 tcgaccatgg atgggcccga gtcgaagcga actcctcgtt ggcggcctcc ggcgtcaccc
     7501 tcaacctgga ggtcgtgcac tcattcggag cgggtggggg tcctatcctg ctgcggtgtg
     7561 acgaccacgg cgatgctgac gactgggagg acgcgaagat catcgcgatc cgccagccaa
     7621 agctccggaa cactgtgtcg ccagcgttcg ccggcacctc ggcgcaggac ccggacgacg
     7681 cgtagaccac aggacgtcga cgacggggcg gccttcgggc cgccccgtcc gcctcctggc
     7741 cgatcaccca gccttcagcc actccacggt ctggcgaagg ccctcctcga gctgggtcca
     7801 cggcttccac cccaggtcgc gctccgccag cgaggggtcc agcgacgacc gacgcaggtc
     7861 gcccttgggg aggggcccga aggagggctc gcccgcgtag ccggtgagct cggccagcag
     7921 acggaacagg tggttgacgc tcgtctccag cccggtcccg atgttgatca gcttccccga
     7981 ccccgcgtcc acggccctgg cgaaggcgtc gacgacgtcc tcgacgtaga cgtagtcgcg
     8041 tgtctggttc ccgtcgccgt tgatctgggg acgctccccg gcgagcatcc gtcgcccgaa
     8101 gatcgccacg acccccgctt cgccgtgcgg gtcctgccgc ggcccgtaga cgttggccag
     8161 ggccagcgag gtgaagtcca gcccctcgta ccgctggaag tacagcaggt agtcgtgcac
     8221 gacccgcttg ctgatgccgt acggggactg cgggtggctc cccgacgtcg ccgtctcctt
     8281 cgcgggcagc ctgcggggct ccccgtagat cgtccccccc gaggccgcgt agacgatctt
     8341 ccgcgtcccc gcacgggcgg cggcctccag gaggttcagc gtccccagca cgttgatgtc
     8401 ggcgtccttg cgcgggtcct cgagcgaggg ccgcacgccg gactgcgccg caaggtgcat
     8461 cacgagctcg ggctggtgct tctcgaacac ggcagggatg gcctcccccc ggatgtcgag
     8521 gttgtagaac tggaagtcct tgccgtagcc ccgggcctcc gccaggttcg cgatccggcc
     8581 caccgagagg tcgtcgatgc cgatcacccg gtacccctgc gcgagcaggc ggtccgagag
     8641 gtgggacccg acgaacccgg ccgcccccgt gacgaggacg gtgcccggcc ggtcgctcat
     8701 gccagcgcca gccccgggtc ggcggccacg tcccagccgt cgcgcgcgcc ccggcggatc
     8761 cggaagtacg cggccgaggc gatcatcgcg gcgttgtcgg tgcagagctc cggcggcgga
     8821 tagtggagtc gcacgccgcg gcggcccgcc tcctcgctca tccgctcgcg gaggcgcgtg
     8881 ttcgcgacca ctcccccacc cagcaggacg gtctccacgc ctcgctcctc cgccaccgcg
     8941 aaggtcttct gcacctgcac gtccacgatc gcctcctgga acgaagccgc gaggtcgccc
     9001 gtcgaaatct cgcgcccggc ctcctgctcg cgtcggacgt agcggatcac cgccgtcttc
     9061 aggcccgaga gggagaagtc gtagtcgcct gatcccgcca tggcccgggg gaagtggatc
     9121 gcctcgggat ccccctcgcg cgccagcttg tccagcgcag gccccccggg gaacccgagg
     9181 cccaggaacc tcgcgatctt gtcgaacgcc tcccccgccg cgtcgtccag cgtctgcccc
     9241 acgacctcgt accggtgctc ctcgggcatg tggacgagca gcgtgtgccc gccggagacg
     9301 atcagggcca cgtacggagg ctccggcggc ccgtgctcca ggaagttcgc gtagacgtgc
     9361 ccctcgaggt ggttcacccc caggaacggg accttcaggg ccagcgcggc ggccttggcc
     9421 gtggccaggc cgacgaggag agcccccacg aggcccggtc cccgcgtcac cgccacggcc
     9481 tcgacgtcgc gccatccgat gccggcctca tcaagggccc gatccatcag ggggcccagc
     9541 cgctcgacgt gggcccgaga ggcgagctcc ggaacgaccc ctccgaagcg cgcgtggacg
     9601 tcgtgctggc tggcgaggag gttcgagtgg atcttcaccc cgtcctccac cacggccacc
     9661 gcggtctcgt cgcaggaggt ctcgagcccg aggaccctca tggcctgcgc agcccctcgg
     9721 gcagggacgc ccggagcccc tccaggaggc gcccgtactc ctcggtccgg atgtcgtcgg
     9781 tccacatcac gagggcgtcc tcaccgatct cctggtagta gttcttccga acgcccaccg
     9841 ggcggaaccc gaaccgcccg tacatccgct gcgccccgtg gttcgtcacc cgcacctcga
     9901 gcgagacggc caccgcctcc atcctcccgg cctgatcgat gagctcgatc aggagacggg
     9961 tcccgatctt gtgccgctgg tgctcggggt ggacggcgat cgtggtgacg tgcgcctcgt
    10021 caccgtagga gaggaggccg ccgtagccga cgacctcgcg gtcgatcagc gccacgtagt
    10081 acgcccgatc cttgtggttg cgaagctccg ccaggaacag gttcgagctc cacggccgcg
    10141 ggtagacctg ccgctcgatc gccagcaccc ccttgaggtg tcggcgacgc atgcgcgtca
    10201 cctcgacctc aggcgctccg cgctcgtcga tcccagttga tctccgcatc cgacttcctc
    10261 aggtagaggg ggaggatctc gtgcgggctg gacgtctcct cccgatggaa ccgcggcacc
    10321 gccagctcga ggagccacac cgccagcggg aaggcgttgg ccgtggaggc gaagtcgacc
    10381 cccgatgcgt cgcgctccag gaccctgcgg tgcatcaggg ctccgccgcc cacgaggagc
    10441 gtctcgtccg gcgaagcttc gagctccgcg gccagccgct cgggagggcc gaccgagggc
    10501 tcggtctcgc gggtcacgcc acccggcacc gaccggtaga aggaccagaa cacctcgctt
    10561 cgcttcgcgt cgaggaccgc gcagatccgc tccctcgcgt accgcatcga gtaggccagg
    10621 acgtcgagcg accccaggcc caccatgggg agcccgagga cctgcgccag ccccttggcg
    10681 gtggagatcc cgacgcggag gccggtgaag aggcccgggc cgatcccgac ggcgacgccg
    10741 cccacctggt cgaggtcgat ctcggaccag gccagcaggc gctcgatcgc ggggacggtg
    10801 acctcggagt ggccgcggcc ccacgagacc acggccgagg ccacgacgcc ctgctcgctc
    10861 ccgatggcca cgctcgtctg gggggtcgat gtctccagcg ccagcaccag catcaggcgg
    10921 ccctccagga gccgagcgag cccgagaggt cgacccagcg ggcggcccac ccggggcccc
    10981 gagcgcgcac cgcgacctcg cgctcctcgt ccccctcgag cgcgtggagc tcgacctcga
    11041 ggtgggagtc tgggagcagc ccctcgatcg cgcctcccca ctcgatgaag acgaccccgt
    11101 ggggatcgag caggtcctcg aagcccaggt ccagcacctc ttggatccgc tccagccgat
    11161 agacgtccac gtggtagatg gggagcgacc cccggtactc gcggacgagc acgaaggtgg
    11221 gggatgccac cgggtcggtc acgccgagct cccgggccga gccctgcacc agcgtcgtct
    11281 tcccggcgcc gaggtcgccg gtgaggctca ccacgtcgcc cgggaccagc gcgcgggcga
    11341 tggccgcgcc cacctcccgc gtctcatccg ccgaccgggt tcgaagccgg atctcgctcc
    11401 ccatcagaag agtccgagct gctcctcgac ggcgggcgct ccctcctcga cggccgcctt
    11461 gatcgcggcg aagtcctccc ggagcgcccg catctgaggc ccgccctcgc cgcccccgtg
    11521 gagggccgcg gcgggatgga acgtcgggat cacggtgcga ccccgccatg cctccctctg
    11581 cccccggacg cgcgagatcg acacctgccg ctgcaggatc acccgcgtgg cgaagttccc
    11641 cagcgtcacg atgacccggg ggtcgaccag atccacctgc tgcatgaggt agggggagca
    11701 ctcctcgatc tcgtccggca tgggatcgcg gttccccggg gggcggcact tcaccacgtt
    11761 ggcgatgtag acgtctcccc gggagagccc gacctcctcc agcagacgat tcaggagctg
    11821 tccggctgcc ccgacgaacg ggacgccctg acggtcctcg tggaagccgg ggccctcccc
    11881 gatgaacatg atcccggcgt tcgggttccc gtctccgaac acgacctggg tccgtccctc
    11941 ggacaggcgg cacctggtgc aggtggatgc ggtccgggcg atctcctcga gctgactcat
    12001 cggcccccct cctcgccggc cggggggatg ccggcgaggc cccgggccag ccgcaccgcc
    12061 cgccgaacgg cgtccgcgag cgcgtcctcg gcgaggcgct cgaggagcga ctgagatgct
    12121 tccacggcgc ccgtcgccag cgtgaagacg gtgtctccgt cccacattgt atgcgccggc
    12181 cggatcgcgc tcgcaaggcc gtcgtgggcg gcgatcgcca ggaggtgggc ccgctcgcgc
    12241 gagagccggg cgtcggtggc cacgacggcc agggtggtgt tcccgaacgg cggcgagggc
    12301 tcctcccctt cctcgaggag ccgcccggcg atcgacgcgc cctcctcgtc cacgacggcg
    12361 cccacggcgt tcaccgcaac gagcgcgccc acggtcacgc cgtcctcggc ggccgaggag
    12421 gtccccacgc cgcccttcac cgcgtgctcc cgccccggca gcttccccac ggtggcgccc
    12481 gtgccggccc ccacgctgcc ctcggggacc tcctccgagg cggcctcgca ggcggcgtac
    12541 ccctggtccg ggcccggccg gacggcgggg tcgcccgcgt ccaggtcgaa gatcacggcc
    12601 gcggggacga tggggacgcg ggcgaccccg ccgtcgaa
//
LOCUS       DASUTZ010000153        15467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_563889, whole genome shotgun sequence.
ACCESSION   DASUTZ010000153 DASUTZ010000000
VERSION     DASUTZ010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 15467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15467
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563889"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1359)
                     /locus_tag="VF097_06655"
     CDS             complement(<1..1359)
                     /locus_tag="VF097_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6262513.1"
                     /translation="MTVLEGLEAVRKRPGMYIGSTGPRGLHHLVYEVVDNAVDEALAG
                     HCTKIKVVLRKDGGVLVEDNGRGIPVTKIPGQKDRRPAVEVVLTTLHAGGKFDGKSYA
                     VSGGLHGVGVSVVNALSRRLEVEVMRDGHTWTQVYERGKRLSALKKGKATKRSGTTVV
                     FWPDPEIFEELEFKRDILAERLQELSFLTGGVEITLVDERERKPWEAVFKARGGLADF
                     VKHINQSKEPIHARVIHFDAKGKDAEVEVAMQWNGSYAESLHSFANTINTHEGGTHEE
                     GFKKALTNVVNRYARAKGLLKEKNENLVGEDIREGLAAILSVKIKDPQFEGQTKTKLG
                     NTPVRSLVETTVNEHFMTWLQENPGPAKRIFQKSEQAAKARIAARQARDLTRRKGLLD
                     SSLPGKLADCQLTDPEVCELFIVEGDSAAGPAIQARDPAYQAILPIRGKILNVEKARL
                     HKI"
     gene            complement(1537..1878)
                     /locus_tag="VF097_06660"
     CDS             complement(1537..1878)
                     /locus_tag="VF097_06660"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEX6262514.1"
                     /translation="MERPRRGDPPSEPTGVGEILRGLLSEGPLRRGLRLGRLGRVWPQ
                     VVGERLAPVTAPVSLDEGGLVVSASTQAWAAQVRFLADEIGRRAAEVLGEDPPPPVRV
                     VVGPERPKRGA"
     gene            complement(1856..2932)
                     /gene="recF"
                     /locus_tag="VF097_06665"
     CDS             complement(1856..2932)
                     /gene="recF"
                     /locus_tag="VF097_06665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HEX6262515.1"
                     /translation="MRLAWIEVRDFRNHARTRLEVPAGLTAVVGANAHGKTNLLEAAH
                     YLLTLSSPRTSADQPLIREGAGSAFLRGEVETAAGRVLVEVEIRPSGANRIQVNRSSV
                     RRKRDLRSRVRSVFFIPEDLAIVAGGPEERRRFLDEAAYGLWPMREGERRAYDRTLRQ
                     RNRLLKEHEGPGAPAELEAWDVELISAGVLVTAARAAAAERVAPRASQRYEGLSGDRL
                     LVAYAPSVAGKDLEAAFREGLAARRRDELIRRTTLVGPHRDDLELAVRGRTARRFASH
                     GEAWAAALCFRLGLTGALEAEVGEPPVLLLDDPFSGIDPERRRRLAASLSGGPQTLLA
                     VPDETQIPDGAVVWEVRDGAATPR"
     gene            complement(2933..4036)
                     /gene="dnaN"
                     /locus_tag="VF097_06670"
     CDS             complement(2933..4036)
                     /gene="dnaN"
                     /locus_tag="VF097_06670"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX6262516.1"
                     /translation="MKFRCERDTLAEALATAQRGVSTRPGIPALTGVMLEAGSEGLTV
                     TTTDLEVTTQVHVPVEVAEEGNALVPARLVAEMVKSLPLDDVDIAADGSTARVTCRSF
                     EGTLRCLPAEDFPQLRQPEGIRLTLEAPAFGEAVAQVVKAASRDEARPILTGVLFEAN
                     REGLTMAATDSYRLAVRELQATGDGEGKALVPERAISEVGRAAAAEEKGEVEVVIGQA
                     QASFRVGELRLISRLIEGEFPNFRQLIPEPGGNKLLAPRQELLEAVRRVGLLARDNAP
                     VRLELNALGLRLTSSSPDLGDAVEAMEASYEGEELTVAFNPSYLGDGLAAVTDERVHV
                     ELRDGLKPAILRGEAGDAYTYLVMPVRLPAPVG"
     gene            complement(4233..5618)
                     /gene="dnaA"
                     /locus_tag="VF097_06675"
     CDS             complement(4233..5618)
                     /gene="dnaA"
                     /locus_tag="VF097_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX6262517.1"
                     /translation="MWKEAVENLPGGLSDPDAHDLWTRAAGAARGRVSPSTYDMWFAD
                     VRGRRLEGDTIELATPTEYARAWLSSHHEDLIRSAVAEAAGRPLDVRLVVEERPEGPP
                     EEPDERPVSEDVPPYQRFPDRYTFETFVLGPSNRFAHAAAMAVAEAPPSKAYNPLFIY
                     GGVGLGKTHLLYAIATHMNKLIEGVRVKYVTSEAFVTEFIRAVRERQGYQFQRRYRDV
                     DVLLVDDIQFLARAEETQTEFFHTFNALHGSGSQIVIASDRPPQELAGLEERLRNRFR
                     WGLCVDVQPPDLETRIAILQKKAERDHMEVPDAVQHFIANKFDMNIRELEGALLRVAA
                     FASLSGVKVDLPLAERALEDLIPQTGQEIPPELIMEQSAQYFSLRRSDLVSKSRSRPL
                     TTARHIAMYLLRELSGLSLIKIGELFDRDHSTALHGIKKIEALMPARGSTYRQVQELT
                     KTVRSLARGGG"
     gene            5830..5964
                     /gene="rpmH"
                     /locus_tag="VF097_06680"
     CDS             5830..5964
                     /gene="rpmH"
                     /locus_tag="VF097_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX6262518.1"
                     /translation="MKRTYQPSNRRRKRRHGFRTRMRTRGGRAILARRRAKGRARLSA
                     "
     gene            5993..6271
                     /locus_tag="VF097_06685"
     CDS             5993..6271
                     /locus_tag="VF097_06685"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_function: GO:0042781 - 3'-tRNA processing
                     endoribonuclease activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX6262519.1"
                     /translation="MGGRPLRSRRVTLYLAPTDQAPRTAVVAGRSVGGAARRNRAKRV
                     IREAWKAVAPRLRESADVVLVARPQIEGATTTELIEEVEDVLTRAGVL"
     gene            6268..6543
                     /gene="yidD"
                     /locus_tag="VF097_06690"
     CDS             6268..6543
                     /gene="yidD"
                     /locus_tag="VF097_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX6262520.1"
                     /translation="MSRALWWAGLPARVLLLGLISGYRRLFRGTYAGRCRFHPSCSAY
                     AEEAIRVHGAFKGGGLALWRVLRCSPLTPGGVDQVPPRRDGVIQGSA"
     gene            6588..7691
                     /locus_tag="VF097_06695"
                     /pseudo
     CDS             6588..7691
                     /locus_tag="VF097_06695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
     gene            7654..8307
                     /gene="jag"
                     /locus_tag="VF097_06700"
     CDS             7654..8307
                     /gene="jag"
                     /locus_tag="VF097_06700"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HEX6262521.1"
                     /translation="MTEEVERSAASVEEAVEAALEELGITEQEAQVEVLQEPQKGLLG
                     IGSKGAVVRVRRRTVLGQATEEQLQVAIDFLSGLMERLGVKAEVEEETHENVQYLDVV
                     AAGDPDVMGLLIGRHGQTLDAIQELTRAAVQRRTAERCRIVVDVEDYRKRRRAQLVER
                     ARDAAEKVQRSGRRHTMEPMTPFERKIVHDVVAEFTELESGSEGQEPKRRVVIRKRR"
     gene            8341..8907
                     /gene="rsmG"
                     /locus_tag="VF097_06705"
     CDS             8341..8907
                     /gene="rsmG"
                     /locus_tag="VF097_06705"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX6262522.1"
                     /translation="MKRLDEYEELLRSRALPMGLIAASDAENLRERHIDDSLRATQAF
                     REDDARCFDLGSGAGLPGIPLAIALPGREFVLIESRRRRAGFLELAIDLLKLENVRVL
                     PDRVESVGERVRAGRTAPADLATARAFASLERSWRAAAPLLRPGGRLVYFGGRGLEDP
                     VGAARSAGVDGEISVLDSSSPLVIMGRR"
     gene            9171..10067
                     /locus_tag="VF097_06710"
     CDS             9171..10067
                     /locus_tag="VF097_06710"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX6262523.1"
                     /translation="MANQKGGVGKSTTAVNLGAALAESGARVLVVDMDPQGNASTGMG
                     VSPQDREITTYQVLAAGLPATRAIMATAVEGLSAITSTIDLAGAEIELVSQFSREMRL
                     ARALDGVVEMFDYVIVDCPPSLGLLTVNSLAAADELIVPIQCEYYALEGLGQLLRNVR
                     LVQQNLNPRLRLTGIVLTMYDSRTKLAEQVVAEVRKYFGRRVYDTLIPRSVRLSEAPG
                     YGQPITTYDPGSKGAKAYRALASEVAQGPREVEAPMPTEDMPEVVLPEPSSEDLDGEE
                     EQEPEPAVKRRRGLFRRKGDES"
     gene            10064..10909
                     /locus_tag="VF097_06715"
     CDS             10064..10909
                     /locus_tag="VF097_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017620594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX6262524.1"
                     /translation="MKQRGGLGRGLSALIPGATDEGGLLEIPLAAIAPNTRQPREAFD
                     EEGIAALASSIREVGVLQPIVVRRREDGYELVAGERRLRAAKVAGLATIPAVIRDTDD
                     TDSLREAIIENIHREDLSPLEQAAAFQELLEDLGASHDELAERLGYSRSHVTNTIRLL
                     QLPPPVQRLVGEGRLSAGHARALLALDDVEGQEALALRVAAEGLSVRQVEELVRTYHG
                     AGERSAERGAPEPDPRAVQVEELLGDALATRVRVQRGRRKGKIVIEFGSDDDLDRIVS
                     AIAGP"
     gene            11000..11560
                     /locus_tag="VF097_06720"
     CDS             11000..11560
                     /locus_tag="VF097_06720"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6262525.1"
                     /translation="MPQVVDVGLENLSSAPPKVLECVFWETEQKDPTDPRFHKEEWFS
                     STLLEWGVCGKLMVDGDEVVAFAQFAPPSLFPRVARYRAGRVSPDAVYLAYCYVASGH
                     RGRGLGSQMIRAVARDLVDRGFRAMESLGQSEWEGGWVLPEAFLAANRFAVVRPDPMV
                     PLMRLELFGVTTPAEARAAEALPLPR"
     gene            complement(11557..12636)
                     /locus_tag="VF097_06725"
     CDS             complement(11557..12636)
                     /locus_tag="VF097_06725"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX6262526.1"
                     /translation="MRSIRRGDRGDEVRDVQARLLALGYVIRQSELEEDRFEGSTDTA
                     VRAFQQDRGLLVDGLVGPQAWEELVEAGYALGDRVLYLRHPPFRGDDVRALQARLNTL
                     GFDPGREDGIFGRQTEVAMRDFQRNVGLPPDGIVGSTSLQALERLRASVPGPGRTMVR
                     ETEDLRLAGSLEGKVVAIDPAHGPGDPGEVGPGGSVESEITYAIAERLAEALEGRGAK
                     PVLLREPGGDPPVAARIDRATASGADLLLSIHLNHHGDPTAEGVAAYYFGRLGAVSVA
                     GRALAEVLLERITSRLGLRDGRAHPKAFPLLRETPMPAVHLEPGFISNPREERLLNEG
                     RFHHDLAEAIVEALERYFAGLAPSD"
     gene            complement(12680..13015)
                     /gene="trxA"
                     /locus_tag="VF097_06730"
     CDS             complement(12680..13015)
                     /gene="trxA"
                     /locus_tag="VF097_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX6262527.1"
                     /translation="MERMSEIMTATDAEWDKVLSSDKPVLVDFWADWCVPCHMVSPIV
                     EEIGREKSDVMSVAKLNVDDNPQTARRYGVMSIPTLILFKDGQEAARVVGAQPKESIL
                     SQIEPYAGA"
     gene            complement(13065..13643)
                     /locus_tag="VF097_06735"
     CDS             complement(13065..13643)
                     /locus_tag="VF097_06735"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX6262528.1"
                     /translation="MITDGMTFPVARSNWHSVITFGRDWHDPRFRMIGGRWRLAGVHE
                     GNDIISEEGTPIHSMTSGVVEAVGWTFYSGTRVGVRGTDGRYYFYAHLSEVALGVAAG
                     SRVEAGTVLGRVGNTGYGDPGTEDEFPPHLHLGIMEGGSWVDPYPTVRRLYRESVQAT
                     TRGERRLARLMRNGDTERYERLAERLYAETEE"
     gene            complement(13768..14694)
                     /gene="trxB"
                     /locus_tag="VF097_06740"
     CDS             complement(13768..14694)
                     /gene="trxB"
                     /locus_tag="VF097_06740"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX6262529.1"
                     /translation="MTERQVVIIGSGPAGFTAGLYAARANLEPLVLKGIEAGGQLMLT
                     TDVENYPGFPEGILGPELMELFEKQAVRFGAEMLQQNATKVDLSRRPFRVWSGDEEHV
                     ARSVIVATGAKAKMLGLPSEQQLLGHGVSTCATCDGFFFRDQELVVVGGGDSAMEEAL
                     FLTRFASKVTVVHRRDELRASKIMQDRAFENPKIGFVWDSVVTDVLGNGKVEGVRLRN
                     VRSGEESELPAGGMFLAIGHTPNTEIFRGQLEMDDVGYLKVEEPTTRTSVEGVFAAGD
                     AVDHIYRQAVTAAGMGCKAAIDAERWLERQGH"
     gene            complement(14747..>15467)
                     /locus_tag="VF097_06745"
     CDS             complement(14747..>15467)
                     /locus_tag="VF097_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262530.1"
                     /translation="ATSARQTLRGLPEVEPPPGTTMAVLREAREARTPSRWARPLRVA
                     AVVILLAGAAAGGIYVYGQLGESGGETAPAGDGGDAAQPEIEAPAAAPEEGSADQDAQ
                     SGRAEALFGGVYPQVRRTGTEHTPDSIVALAEDLRDEALEHLEAGFPPDAFAFYEAFP
                     VASFQPAARDALACAGQGLEPERSVAPYVIEDASWGGRPAWVVSYLQGPEPAAAFDRV
                     QILVVDRESCILAHFAQQRLD"
BASE COUNT         2246 a         5391 c         5496 g         2334 t
ORIGIN      
        1 gatcttgtgg aggcgggcct tctccacgtt gaggatcttc ccgcggatgg ggaggatcgc
       61 ctggtaggcg gggtccctgg cctggatcgc cggcccggcc gccgagtccc cctccacgat
      121 gaacagctcg cacacctcgg ggtccgtgag ctggcagtcg gcgagcttcc ccgggagcga
      181 cgagtccagc aggcccttcc gccgcgtgag gtcgcgggcc tggcgcgccg cgatcctggc
      241 cttcgcggcc tgctcggact tctggaagat gcgcttggcc ggcccaggat tctcctggag
      301 ccaggtcatg aagtgctcgt tgacggtcgt ctccaccagc gagcggactg gcgtgttgcc
      361 cagcttcgtc ttggtctggc cctcgaactg aggatccttg atcttcaccg agaggatcgc
      421 ggcgagccct tcgcggatgt cctcgcccac gaggttctcg ttcttctcct tgagcagccc
      481 cttggcccgc gcgtagcggt tgacgacgtt ggtcagggcc ttcttgaagc cctcctcgtg
      541 ggtcccgccc tcgtgggtgt tgatcgtgtt ggcgaacgag tggagcgact ccgcgtagct
      601 gccgttccac tgcatcgcca cctcgacctc ggcgtccttc cccttggcat cgaagtggat
      661 cacgcgggcg tggatcggct ccttggactg gttgatgtgc ttgacgaagt cggcaaggcc
      721 tcccctggcc ttgaacacgg cctcccacgg cttccgctcg cgctcgtcga cgagggtgat
      781 ctccacgccc cccgtgagga aggagagctc ctggagccgc tcggcgagga tgtcgcgctt
      841 gaactcgagc tcctcgaaga tctccgggtc gggccagaac accacggtcg tgcccgaccg
      901 cttcgtggcc ttccccttct tcagcgcgga gaggcgcttg ccccgctcgt agacctgggt
      961 ccaggtgtgc ccgtcccgca tcacctccac ttcgaggcga cgggagagcg cgttcaccac
     1021 ggagacgccc acgccgtgga ggccgcccga gaccgcgtag gacttcccat cgaacttccc
     1081 gccggcgtga agggtggtga ggaccacctc caccgcgggg cgccggtcct tctggccggg
     1141 gatcttggtg acggggatcc cacgcccgtt gtcctcgacg aggacgcccc cgtccttcct
     1201 gagcaccacc ttgatcttgg tgcagtggcc ggcgagggcc tcgtccacgg cgttgtccac
     1261 gacctcgtag acgagatgat ggaggccgcg gggcccggtc gacccgatgt acatcccggg
     1321 tcgcttgcga accgcttcga gtccctcgag gacggtgatg tctgcagccg tgtagccgct
     1381 cttgcgggtc cttttcgtcg tcttcgaggt cttttgcgcc acgtaccctc cgtgatggcc
     1441 ccgcgcacag ggtgaaggcg gccgtaccga aaggggctgc accgcgcggg tttcgtcgat
     1501 gtgattatac catcgaggac cgtcgccccc gccctgtcag gcccctcgct tgggccgctc
     1561 cgggcccacc acgaccctca ccggcggggg cggatcctct cccagaacct ccgccgcccg
     1621 ccgcccgatc tcgtctgcca agaagcgcac ctgggctgcc caggcctgcg tcgaggccga
     1681 caccacgagg cctccctcgt ccagggagac gggcgccgtg acgggggcca gccgctcccc
     1741 caccacttgg ggccagaccc gtcccaggcg ccccaagcgc agacctcggc gaaggggccc
     1801 ctcgctcagc aggcctcgca ggatctcccc caccccggtg ggctcggacg gcgggtcacc
     1861 tcggcgtggc cgctccatca cgcacctccc acacgacggc tccatccggg atctgggtct
     1921 cgtcgggcac ggcgagcagg gtctggggac cgccgctcag cgaggccgcg agacggcgcc
     1981 ggcgctccgg gtcgatcccc gagaaggggt cgtccaggag cagaaccggc ggctccccca
     2041 cctcggcctc gagggccccg gtcagcccca gccggaagca cagggccgcc gcccaggcct
     2101 ccccgtgcga cgcgaacctc cgcgccgtcc gacctcggac ggccagctcg aggtcgtctc
     2161 ggtgaggccc cacgagcgtt gtgcgcctga tcagctcgtc ccggcgacga gcggccagcc
     2221 cctcccggaa ggcggcctcc aggtccttcc ccgccacaga gggggcgtag gcgaccaaga
     2281 gacggtcccc cgagagcccc tcgtaccgct gggacgccct gggggcgacg cgctcggcgg
     2341 ccgccgccct ggccgccgtg accagcacgc cggccgagat cagctcgacg tcccaggcct
     2401 cgagctccgc cggtgccccc ggcccctcgt gctccttgag caggcggttc cgctgcctca
     2461 gcgtccggtc gtaggcccgc cgctccccct cccgcatcgg ccagaggccg taggccgcct
     2521 cgtccaggaa gcgccgacgc tcctcggggc cgccggcgac gatcgccagg tcctcgggga
     2581 tgaagaacac gcttcggaca cggctccgga ggtcgcgctt ccttctgacc gacgaccggt
     2641 tcacctggat gcggttggcc ccggagggac ggatctcgac ctcgacgagc acgcgtccgg
     2701 ccgccgtctc gacctcgccc cgaaggaagg cggatcccgc cccctcccgg atcagaggct
     2761 gatcggcaga cgtccgaggc gacgacagcg tcagcaggta gtgcgcggcc tcgaggaggt
     2821 tcgtcttccc gtgggcgttc gcgccgacca cggcggtcag cccggctggc acctcgagcc
     2881 gggttcgggc gtggttccgg aagtcccgga cctcgatcca cgcgagccgc acctagccca
     2941 cgggggcggg cagacgcacc ggcatcacga gataggtgta ggcatcgccg gcctccccgc
     3001 ggaggatcgc cggcttcagc ccgtcccgca gctccacgtg cacgcgttcg tcggtcaccg
     3061 ccgccagccc gtcccccagg taggacgggt tgaaggccac ggtcagctcc tcgccctcat
     3121 agctcgcctc catcgcctcg accgcgtcgc cgaggtcggg cgaggaggag gtcaggcgca
     3181 ggccaagcgc gttcagctcg aggcgcacgg gcgcgttgtc gcgcgccagg aggccgacgc
     3241 gacgcaccgc ctcgagcaac tcctgcctcg gagccaggag cttgtttccg ccgggctcgg
     3301 ggatgagctg acggaagttc gggaactcac cctcgatcag cctcgagatc aagcggagct
     3361 ccccgacccg gaacgaggcc tgcgcctgcc cgatcaccac ctccacctcg cccttctcct
     3421 ccgccgccgc ggcccggccc acctcgctga tggcccgctc ggggacgagc gccttcccct
     3481 cgccgtcgcc ggtggcctgc agctctcgga cggccagccg gtaggagtcg gtggcggcca
     3541 tggtgaggcc ctcgcggttc gcctcgaaca gcacgcccgt caggatcggg cgggcctcgt
     3601 cgcgggacgc cgccttgacc acctgcgcca ccgcctcccc gaaggccggg gcctccaggg
     3661 tcagacggat cccctccggc tggcggagct gggggaagtc ctcggccggc aggcagcgca
     3721 gggtgccctc gaagctccgg caggtcaccc gagcggtcga gccgtccgcc gcgatgtcga
     3781 cgtcgtcgag gggcagggac ttcaccatct ccgcgaccag cctcgccggt acgagggcgt
     3841 tcccctcctc ggcgacctcc accgggacgt gcacctgcgt cgtgacctcg agatcggtcg
     3901 tggtgacggt gagaccctcg ctccccgcct cgagcatcac ccctgtcaga gcggggatcc
     3961 ccggacgggt cgagaccccc cgctgcgcgg tcgccagcgc ctcggctagc gtgtcgcgtt
     4021 cacacctgaa cttcacaggc cacctcccgc tctcgagaaa cgccttcttc ctttccaaac
     4081 cactgacagt cgtaggggct gtggaactgt ggagaaccca tcgttcgccc agggtagcgc
     4141 gggggtctga catgggacgt tctggggacg ggctcgggat gaggcgcgcg ccggagcgcc
     4201 acccaccgag aggccgcgat cttctccaca ggtcatcccc cgcctctcgc cagcgatcgg
     4261 accgtcttcg tcagctcctg cacctgcctg tacgtcgagc cgcgagcggg catcagggcc
     4321 tcgatcttct tgatcccgtg aagcgcggtc gagtgatcgc ggtcgaacag ctcgccgatc
     4381 ttgatcaggg agagccccga gagctcacgc aggaggtaca tggcgatgtg ccgggcggtg
     4441 gtcagaggac gagacctgct cttggacacc agatcagagc gccggaggct gaagtactgg
     4501 gccgactgct ccatgatgag ctcgggcggg atctcctggc ccgtctgggg gatcaggtcc
     4561 tcgagagcgc gctcggcgag cggcaggtcc accttcacgc ccgagagcga ggcgaaggcg
     4621 gcgacccgca gcagcgcccc ctcgagctcg cggatgttca tgtcgaactt gttggcgatg
     4681 aagtgctgga cggcgtcggg gacctccatg tggtcccgct cggccttctt ctggaggatg
     4741 gcgatccgcg tctcaaggtc ggggggctgg acgtccacgc agaggcccca cctgaaccgg
     4801 ttgcggagcc gctcctcgag ccccgccagc tcctggggcg gccggtcgct ggcgatcacg
     4861 atctggctcc ccgagccgtg gagcgcgttg aacgtatgga agaactcggt ctgggtctcc
     4921 tcggcccggg ccaggaactg gatgtcgtcg acgagcagca cgtccacgtc acggtagcgc
     4981 cgctggaact ggtagccctg ccgctcccgg acggcccgga tgaactcggt gacgaaggcc
     5041 tccgaggtga cgtacttcac gcggacgccc tcgatcagct tgttcatgtg ggtggcgatc
     5101 gcgtagagca gatgggtctt ccccagcccc actcccccgt agatgaagag agggttgtag
     5161 gccttggagg gcggggcctc ggccaccgcc atcgccgcgg cgtgtgcgaa ccggttcgac
     5221 gggccgagga cgaaggtctc gaacgtgtag cggtcgggga agcgctggta cggggggacg
     5281 tcctcggaga ccgggcgctc gtccggttcc tccggtgggc cttccgggcg ctcctcgacc
     5341 accagccgga cgtcgagggg tcggcccgca gcctccgcca ccgcgctccg gatcaggtcc
     5401 tcgtgatggg acgacagcca ggcccgtgcg tactcggtgg gggtggccag ctcgatcgtg
     5461 tctccctcga gccggcggcc gcgcacgtca gcgaaccaca tgtcgtaggt gctcggcgag
     5521 acccgtccgc gcgcggcgcc cgccgctctc gtccacaggt catgggcgtc ggggtcgctc
     5581 agccctcccg ggaggttttc cacagcctct ttccacatgt gtgaaatccg ccggggaagt
     5641 acaagggggc cgccggaagg cgaccccagg gaagcgacgc ggatattagc acccccctca
     5701 cgcggaaagc aatggggcgg cgctgacctg cggcttcgtg ttcgctcgtt cgggtccggc
     5761 gcgcatcgcc gcaggtcagc gccgcccacg tggaccggcc ccgtcggcgg tgcgtatact
     5821 cccgtattcg tgaagcgcac ctatcagccg agcaatcgca gacgcaagcg ccgccacggc
     5881 ttcaggaccc gtatgcgcac ccgggggggc cgcgcgatcc tcgccaggcg acgggcgaag
     5941 ggccgtgccc gcctcagcgc gtagcctcag gggaagcgag atccgagggc tgatgggcgg
     6001 gaggccgctg cgctcgaggc gcgtcacgct gtacctggcc ccgaccgacc aggccccccg
     6061 aacggccgtc gtggccggga ggtcggtggg tggggcggcc cggaggaacc gggcgaaacg
     6121 ggtgatccgg gaggcctgga aggcggtggc cccgaggctg agggagtccg ccgacgtggt
     6181 cctggtcgcg cgaccgcaga tcgagggagc cacgacgacg gagctgatcg aggaggtcga
     6241 ggacgtgctg acgcgggcgg gcgtgttgtg agtcgggccc tctggtgggc cggcctgccc
     6301 gctcgagtcc tcctcctggg gctcatcagc ggataccgga ggctcttccg cgggacgtac
     6361 gcggggcgct gccgcttcca cccgtcgtgc tcggcgtacg ccgaggaggc gatccgagtg
     6421 cacggggcct tcaagggagg cggtttggcc ctgtggcgag tgctccggtg cagccctctg
     6481 accccgggcg gggtggacca ggtcccgccc aggcgtgacg gggtcataca ggggtccgcc
     6541 tgatggagca gatcgccggc gtccttctgc agaccctggg ctacgcgctc agcttcctgt
     6601 acgacgtggt cccggtgtac ggggtggcga tcgtgctgct cacgatcgcg gtgcggctgc
     6661 tgctcctccc cctcaccatc aagcagaccc gctccatgca ggcgatgggg cggctccagc
     6721 ccaagatgaa ggagctccag cggaagtaca agggggaccg acagaagctg aacgaggaga
     6781 tgatgaagct ctaccgggag cacggcgcca acccgctggg gggctgtctc cccctcctcc
     6841 ttcagctccc cgtgttcttc gccctctatg cggtgctgcg ggcggccacg cccgagatcg
     6901 cggtccccgc ggagccggtc tccttcgggg cggtggagga tgcgttctgc cggccggtgg
     6961 gcgagccggg tatcagcggg accgcgccga atgagctgga gtgcgactcg gatggcgacg
     7021 ggaacttcga gaaggcgatc ccggtctccg agtggcggga gcgggatgga gagccgatcc
     7081 agcctcggga cgtcctgacc cgatgcagcc cggagcgcga gggcgggcag gtgcggttcc
     7141 gctgcgagac gccggtgggc acgggacacc tccccgagga cggcgccctc ttcgaggcga
     7201 tcatcgagga cggcgagccg ttcctcggga tgtacctctc ctgctcggcc acgcaggccc
     7261 agtcggagga ggccttccgg cagtgcgcgc cgaggggggc cgaagggagc gctctgaccc
     7321 tggtgggata ctatgggctg atcgccctga tggtcctctc caccttctac cagacgaggc
     7381 agatgcaggc ggtctcaggg agccagcagc cgggcgctca gacgatcggg cggatcatgc
     7441 cgatcttcct ggggttcatc tcgctctcca tcccggccgg ggtcctggtg tattgggtga
     7501 ccacgaacat gtggcagatc ggccagcagt acgtgatgat caagaagcgc gaggcgggtg
     7561 aggcgcaggc tgcccccgcc aagccgaccg cggccaaggg cgacggcgag gaccccgagc
     7621 ccaagccgcc gccccggaag agcggaggac gtcgtgacgg aagaggtcga aagaagcgcc
     7681 gcaagcgttg aggaggcggt cgaggccgcc ctcgaggaac tcggcatcac cgagcaggaa
     7741 gctcaggtcg aggtcctcca ggagcctcag aagggcctct tggggatcgg gagcaagggc
     7801 gcggtggtca gggttcgtcg gcgcaccgtg ctcggccagg ccaccgagga gcagcttcag
     7861 gtggccatcg acttcctctc cggcctgatg gagcggctcg gagtgaaggc cgaggtcgag
     7921 gaggagaccc acgagaacgt gcagtacctc gacgtcgtgg ccgccggcga tcccgacgtg
     7981 atgggcctcc tgatcgggag gcacggccag accctcgacg cgatccagga gctgaccagg
     8041 gcggccgtgc agcggcgaac cgccgagcgg tgccggatcg tggtggacgt cgaggactat
     8101 cggaagcgac gacgcgccca gctggtcgag cgggcccgcg atgccgcaga gaaggtccag
     8161 aggtccggtc gccgccacac gatggagccg atgacgccct tcgagcggaa gatcgtgcac
     8221 gacgtcgtag cggagttcac cgagctggag tcggggagcg aggggcagga gccgaagcgg
     8281 cgcgtggtga tccgcaagcg gcgataggtt tcacgtgaaa ccctcctccg tcggttccac
     8341 gtgaaacgtc tcgacgagta cgaagagctc cttaggtccc gggccctccc gatgggactg
     8401 atcgccgcct ccgacgcgga gaacctcagg gagaggcaca tcgacgactc ccttcgcgcg
     8461 actcaggcct ttcgcgaaga cgacgcgcgc tgcttcgatc tgggctcggg cgccggcctc
     8521 ccggggatcc cgctcgccat cgcgctcccc ggtcgcgaat tcgtgctgat cgagtcccgg
     8581 cgccgccgag ccggattcct ggagctcgcc atcgacctgc tgaagctgga gaacgtccgg
     8641 gtgcttcccg atcgcgtcga gtcggtgggg gagcgggtcc gggccgggcg gacggctccg
     8701 gccgacctgg ccacggcccg cgccttcgct tccctggagc gtagctggag ggccgccgcc
     8761 cccctcctca ggcccggcgg ccgcctcgtg tatttcggtg gccggggact ggaggatccc
     8821 gtgggggcgg cccgctcggc gggcgtggac ggggagatct cggttcttga ttcctcgtcg
     8881 ccgctcgtta tcatggggcg gcggtgagcc tcgacgacga gcggcctcac gcggagcctt
     8941 ccccgccgga cgggggatcg cagcacgccc ccagcgagcc ggacgtggag catgcgcccc
     9001 aggagcgtcc gatgcctgag ggcgaggccc aggccgaggc gccggagccg cggccggagc
     9061 agacgccggg cccccccatc ccgccgcagc ccactccccc agaccctcaa gttcgagtcg
     9121 agggttccta cgagcagcca gccgctgaga cccgcggcag gatcatcgcg gtggccaacc
     9181 agaaaggagg cgtggggaag agcaccacag ccgtgaacct cggcgcggcc ctggccgagt
     9241 ccggggcacg cgtcctggtc gtggacatgg atccacaggg gaacgcgtcc accgggatgg
     9301 gggtctcgcc gcaggaccgc gagatcacca cctatcaggt tctggccgcg ggacttccgg
     9361 ccacccgtgc catcatggcg accgcggtgg agggcctctc cgcgatcacc tcgaccatcg
     9421 atctggcggg ggccgagatc gagctcgtga gccagttctc gagggagatg cggctggccc
     9481 gagcgctgga cggggtggtg gagatgttcg actacgtgat cgtggactgc ccaccctcgc
     9541 tcggcctcct caccgtgaac tccctggccg cggccgatga gctgatcgtc cccatccagt
     9601 gcgagtacta cgcgctggag gggctggggc agctgcttcg gaacgtgagg ctggtccagc
     9661 agaacctcaa cccgagactg cggctgaccg ggatcgtgct gacgatgtac gactcgagga
     9721 cgaagctggc cgagcaggtc gtggcggagg tccggaagta cttcgggcga agggtgtatg
     9781 acaccctcat acctcggagc gtgcggctgt cggaggcacc ggggtacggc cagccgatca
     9841 cgacctacga cccgggatct aagggggcga aggcgtacag ggcgctggcg tcggaggtgg
     9901 ctcagggtcc gcgggaggtc gaggccccca tgccgaccga ggacatgccg gaggtggttc
     9961 tccccgagcc cagctcggag gacctcgatg gagaggaaga gcaggagccc gagccggccg
    10021 tcaagaggag gcgggggctc ttccggagga agggagacga gtcgtgaaac agcggggtgg
    10081 actcggaagg gggctctcgg ccctgatccc gggggcgacc gacgagggtg ggctcctgga
    10141 gatcccgctc gccgcgatcg cccccaacac ccgtcagccg agggaggcgt tcgacgagga
    10201 aggcatcgct gcgctggcca gctcgatccg ggaggtgggc gtcctccagc ccatcgtggt
    10261 gaggcggcgc gaggacgggt acgagctggt ggctggggag cggcggctgc gcgcggcgaa
    10321 ggtggccggc ctggccacga tccctgcggt gatcagggac accgacgaca ccgactcgct
    10381 gcgcgaggcg atcatcgaga acatccaccg ggaggacctc tcccccctgg agcaggccgc
    10441 cgcgttccaa gagctgctgg aggacctcgg ggcctcgcac gacgagctgg cggagcgtct
    10501 ggggtactcg cggtcccacg tgacgaacac gatccggctg ctgcagctgc cgcccccggt
    10561 ccagcgcttg gtgggggagg ggcggctgtc ggcgggccac gctcgcgccc tgctggccct
    10621 ggacgacgtg gagggtcagg aggcgctcgc cctccgcgtg gcggcggagg gactctccgt
    10681 ccgccaggtg gaggagcttg tgcgcaccta ccacggagcg ggtgagcgga gtgctgagcg
    10741 gggggctccg gagcctgacc cgcgcgccgt ccaggtcgag gagcttctgg gggacgctct
    10801 cgcgacgcgt gtcagggttc agcggggacg gcgtaagggg aagatcgtca tcgagttcgg
    10861 gtccgacgac gacctggaca ggatcgtctc agcgatcgcg ggaccatgag cccggagggg
    10921 cggcgagccg cgaaggccct cgccctcgga gcgctggcgg gcgccctgat cgccttgctc
    10981 gcccgtcgac ggggctgacg tgccccaggt cgtcgacgtc ggtctggaga acctgtcctc
    11041 ggcgccgccc aaggtcctgg agtgcgtgtt ctgggagacc gagcagaagg atcccacgga
    11101 cccccggttc cataaggagg agtggttctc ctccacgctg ctggagtggg gggtctgcgg
    11161 gaagctgatg gtcgacggcg acgaggtagt ggccttcgct cagttcgctc caccatcgct
    11221 cttcccgcgc gtcgcccgct accgggcggg tcgcgtctcg ccggacgcgg tgtacctggc
    11281 ctactgttac gtggcgtcgg gccaccgcgg gcgtggcctg ggctcccaga tgatccgggc
    11341 ggtggcccgc gacctcgtgg accgcggctt ccgcgccatg gagtcgctgg ggcagtcgga
    11401 gtgggaagga ggatgggtgc tccccgaggc cttcctcgcc gcgaatcgct tcgccgtggt
    11461 ccggccggac ccgatggtac cgctgatgcg cttggagctc ttcggtgtga cgacgcccgc
    11521 cgaggcccgc gccgccgagg cgctccccct gcctcgctag tcggagggcg ccagccccgc
    11581 gaagtacctc tcgagggctt ccacgatcgc ctccgccaga tcgtggtgga accgaccctc
    11641 gttcaggagg cgctcctccc gggggttcga gatgaagccg ggctcgagat ggaccgccgg
    11701 catcggggtc tcccggagga gggggaaggc cttggggtgg gcccggccgt cgcggaggcc
    11761 gagccgagac gtgatccgct ccaagagcac ctcggccagg gcgcgccccg ccaccgagac
    11821 ggcgccgagg cggccgaagt agtaggcggc caccccctcg gccgtgggat ccccgtgatg
    11881 gttgaggtgg atcgagagga ggaggtcggc gccggaggcg gtcgctcggt cgatccgagc
    11941 ggccaccggg gggtccccgc cgggctcgcg cagcagcacc ggcttcgcgc cacgcccctc
    12001 gagcgcctcg gccaggcgct cggcgatggc ataggtgatc tcgctctcga cggatcctcc
    12061 cgggcctacc tccccggggt cccctggacc gtgcgcggga tcgatggcga ccaccttccc
    12121 ctcgagcgag ccggccagcc ggaggtcctc ggtctctcgg accatcgtcc gacccggtcc
    12181 agggaccgag gcccggagcc gctccagcgc ctggaggctc gtgctcccca cgatgccgtc
    12241 gggggggagc cccacgttcc gctggaagtc gcgcatcgcg acctcggtct ggcggccgaa
    12301 gatcccgtcc tcccgtccgg ggtcgaaccc gagggtgttg agccgagctt ggagagcccg
    12361 gacgtcgtcc cctcggaacg gggggtgccg caggtacagg acacggtcgc cgagcgcata
    12421 gccggcctcg acgagctcct cccatgcctg ggggcccacg agcccgtcca ccagcagccc
    12481 tcgatcctgc tggaacgccc ggacggccgt gtcggtggac ccctcgaacc gatcctcctc
    12541 cagctcgctc tgtctgatca cgtagcccag ggcgagcagg cgcgcctgga cgtcgcggac
    12601 ctcgtcgccg cggtcgcctc ggcgaatgct cctcacggat cgagtatgtg ccgcccgggg
    12661 ggcggcgaca acgccgggct caggctcccg cgtacggctc gatctggctg aggatggact
    12721 ccttgggctg ggctccgacc accctggccg cctcctgacc gtccttgaag aggatcagcg
    12781 tggggatgct catcacgccg tagcggcggg cggtctgcgg gttgtcgtcg acgttcagct
    12841 tggccaccga catgacatcg gacttctccc ggccgatctc ctccacgatg ggagacacca
    12901 tgtggcaggg cacgcaccag tccgcccaga agtccacgag cacgggtttg tcggaagaga
    12961 gcaccttgtc ccactcagca tccgtggcgg tcatgatctc gctcatccgt tcgattcctc
    13021 ctcggtactg cgggcgccta cctgcaacga cgtgcgtggg gcggctattc ctccgtctca
    13081 gcgtacagcc tctccgccag ccgctcgtac ctctccgtgt cgccgttccg catgagccgc
    13141 gcgaggcgcc gttctccccg ggtcgtggcc tgcacgctct cgcgatacag ccgtcgcacc
    13201 gtcggatagg ggtctaccca ggagccgccc tccatgatcc cgaggtggag gtggggcggg
    13261 aactcgtcct ccgtcccggg gtccccgtag cccgtgttgc ccacccggcc caggaccgtg
    13321 cctgcctcga cccgtgaccc ggccgcgacg ccgagagcga cctcggagag gtgggcatag
    13381 aagtagtagc ggccatccgt ccctcgcacc cccacccgcg tccccgagta gaacgtccag
    13441 cccaccgcct ccacgacccc ggaggtcatc gaatggatgg gcgtgccctc ctcggagatg
    13501 atgtcgttcc cctcgtggac cccggcgagc cgccagcgcc ccccgatcat gcggaaccga
    13561 ggatcgtgcc agtcgcggcc gaaggtgatg accgagtgcc agttcgaacg ggccaccggg
    13621 aaggtcatcc cgtccgtgat cacgggccgg tagtaggcag ggggcggttg gagaggagag
    13681 tcctgggcgg gagccgtgga cggggggacc gtcaagaccg caagcccgag gaccgcgagg
    13741 gtggtggctc ggcgcatccc gaggcggtca gtggccctgg cgctcgagcc accgctcggc
    13801 gtcgatcgcg gccttgcacc ccatcccggc cgcggtcacg gcctgccggt agatgtggtc
    13861 cacggcgtcg ccggcggcga agacgccctc cacgctggtc cgcgtcgtcg gctcctcgac
    13921 cttgaggtag cccacgtcgt ccatctctag ctggcccctg aagatctccg tgttgggcgt
    13981 gtgtccgatc gccaggaaca tgccgcccgc ggggagctcg gactcttctc cggacctgac
    14041 gtttcgcagc cggacgccct cgaccttgcc gttccccagg acgtccgtga cgaccgagtc
    14101 ccagacgaac ccgatcttcg ggttctcgaa ggcccggtcc tgcatgatct tcgaggcccg
    14161 gagctcgtcc cgccggtgca cgaccgtgac cttcgaggcg aagcgggtaa ggaagagcgc
    14221 ctcctccata gccgaatcgc ctccgcccac gaccacgagc tcctggtcac ggaagaagaa
    14281 gccgtcgcac gtggcgcagg tcgagacacc gtgacccagc agctgctgct ccgaggggag
    14341 gcccagcatc ttcgccttgg cgcccgtggc cacgatcacc gaccgggcga cgtgctcctc
    14401 gtctcccgac cagacccgga aggggcgccg ggagaggtcc accttcgtgg cgttctgctg
    14461 cagcatctcc gccccgaagc ggaccgcctg cttctcgaag agctccatga gctcggggcc
    14521 gaggatcccc tcggggaacc ccgggtaatt ctcgacgtcg gtggtgagca tgagctggcc
    14581 gccggcctcg atgcccttga ggacgagcgg ctcgagattg gcccgagccg cgtagaggcc
    14641 ggcggtgaag cccgccggcc cggacccgat gatgacgacc tgtcgctcgg tcactcgctc
    14701 attctcccag gagacgcccg atcatccaac gcgggggggc gttcgtttag tccaggcgct
    14761 gctgggcgaa gtgggcgagg atgcacgact cccgatccac cacgaggatc tggacccgat
    14821 cgaaggcggc ggcgggctcg ggcccctgga ggtacgagac gacccaggcc ggccggcccc
    14881 cccacgaggc gtcctcgatc acgtacggag cgacgctgcg ctcgggttcc aggccctggc
    14941 cggcgcaggc cagggcgtcc cgggccgccg gctggaacga ggcgaccggg aaggcctcgt
    15001 agaaggcgaa ggcgtccggt gggaagccgg cctcgaggtg ctccagcgcc tcgtcccgta
    15061 ggtcctcggc cagcgccacg atcgagtccg gggtgtgctc ggtgccggtg cgacggacct
    15121 gcgggtacac cccgccgaag agcgcctccg ctcggccgct ctgggcgtcc tggtccgcgc
    15181 ttccttcctc cggcgccgcg gccggcgcct cgatctccgg ttgggcggcg tcgcccccgt
    15241 ccccggccgg ggcggtctcc ccgccgctct ccccgagctg accgtacacg tagatgcctc
    15301 cggctgcggc cccggccagg aggatgacga cggctgcgac gcgcagaggc cgtgcccagc
    15361 gcgagggcgt ccgcgcttcc cgggcctcgc ggaggacggc catcgtggtt ccggggggtg
    15421 gctcgacctc gggaaggcct cggagggtct gtcgggccga ggtggcc
//
LOCUS       DASUTZ010000154         5653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_823065, whole genome shotgun sequence.
ACCESSION   DASUTZ010000154 DASUTZ010000000
VERSION     DASUTZ010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5653
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823065"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VF097_06750"
     CDS             complement(<1..350)
                     /locus_tag="VF097_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6262531.1"
                     /translation="MTETYKLILAGEEADAASGETFESTDPSTGKPFATVAKAGPEDV
                     QRAAEAARRAFDEGPWPRMKGSERAQVLMDVASRIKEQGKELAETEAQDSGGTIRKGR
                     FSDVAMAISTFRVYA"
     gene            423..2060
                     /locus_tag="VF097_06755"
     CDS             423..2060
                     /locus_tag="VF097_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6262532.1"
                     /translation="MDPFVQEPPQPPDRFREDRPLRHALERLLPAEVFEEALPELADM
                     AGIATGELQELADRAEAHPPRHVPFDAWGRRVDLVEVGPAWTRLVEIGQERGVVALPY
                     EDRFGEHARVVQLGLLSLYLPVSATADCPLAMTDAAVRVLLNEDPELASRYVPRMTSR
                     RDAWTSGQWMTETTGGSDVGRTETVARDQGDGTWTLHGTKWFTSATTADVALALARPE
                     GAEEGSRGLSLFLLELRDGDRWNGITVRRLKDKLGTRALPTAELDLDGTVAVPVGGIG
                     RGVAKIAAMLNVTRLHAAAGSLGAAGYGLSLARDYAYRREAFGRKLVDLPVHRGWIAR
                     VAAEYEAMNALAFRAAELLGAVERGGGVPELARVAIPLTKMALGRQGVWATSHLIESF
                     GGMGYLEDSGLPRLLRDAHVQSIWEGTTSVLALDVLRALGASGVAEAFAQDVAARTRA
                     ATGSILEEPAARVLAALETLRPMLAEPSEPQARRLAWGMARTYQAALLCEAAAWALET
                     HGDDRAATALRLFSSEPLVGEEPPADPDALGRLAFGR"
     gene            2107..2449
                     /locus_tag="VF097_06760"
                     /pseudo
     CDS             2107..2449
                     /locus_tag="VF097_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730828.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            complement(2484..2870)
                     /locus_tag="VF097_06765"
                     /pseudo
     CDS             complement(2484..2870)
                     /locus_tag="VF097_06765"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            2942..3862
                     /gene="paaA"
                     /locus_tag="VF097_06770"
     CDS             2942..3862
                     /gene="paaA"
                     /locus_tag="VF097_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HEX6262533.1"
                     /translation="MTSYDERTAEFKARIDAGEKIEAGDWMPDEYRRSALKFIEMHAN
                     SEVMGCLPEREWIPRAPSLRRKLSLTAKVQDEAGHGQILYRVAEDLGKSREQMFDDLV
                     NGRTKFHNVFHYPAETWGDVAVIGWLIDGAALVTQKALLDCSYAPYVRAMKRICAEES
                     LHLRHGEDLTLELVSGTDEQRELFQDAVNRWWKPIMHFFGPPSKPAKDVLLYWGIKTR
                     TNEDLRQEFFSTYVPKLWDLGISVPDPELRYDEEAGEWIWSDPDWDEFWQVVKGNGPM
                     TQTRLFWRKAIWDHHAWVRETFDRIPAAAV"
     gene            3867..4247
                     /locus_tag="VF097_06775"
     CDS             3867..4247
                     /locus_tag="VF097_06775"
                     /inference="COORDINATES: protein motif:HMM:NF018002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262534.1"
                     /translation="MIVWDVLQRRRAGADWEVVGGVKAPDADFAILLARESLFRRREG
                     ETYAVRRRGTEDLIEITDPIGIGGVIDREFRRMDSFAGVGGKHRRIHEEMRERGLVID
                     RPRPPDRRQRRSAKQGPSEETARA"
     gene            4240..5109
                     /gene="paaC"
                     /locus_tag="VF097_06780"
     CDS             4240..5109
                     /gene="paaC"
                     /locus_tag="VF097_06780"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEX6262535.1"
                     /translation="MPEGDPRLILLLALADDELIIGHRHSEWTGWAPHLEEDLAFSSI
                     AQDEIAHARVLLELAEPLAGRGVDELALGRSPGEYRHAVVCERPNGHWGYTVARQYLY
                     DTADDVRLAALEGSSDADLAAAVKTIRLEERYHVEHARIWFRRLAEGPVQAREHLSEG
                     LEQAVPEALALFEELPGEEALVADGVLPSAMEDLLSTWLEGLGADLESVSLDYVLSRH
                     KRVGELVPTSSGEAQEGSTLEVPGLERRGDRWVAVGGFAGAGGRRGKHTEDFDELWEE
                     MTMLYRKVPGATW"
     gene            5106..5627
                     /gene="paaD"
                     /locus_tag="VF097_06785"
     CDS             5106..5627
                     /gene="paaD"
                     /locus_tag="VF097_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015375105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HEX6262536.1"
                     /translation="MTPIAAADVRDRVRRALERVMDPEIPTASIVDLGMVERVEVRDD
                     LVEVDLLPTFVGCPAKDVIGEDAEAAVREAAGDRDVRVRFVFDPPWTTDRITEKGKAA
                     LREYGISPHWEGGPPDRGAVVVPLLAKPAGIPCPYCGSDRTVTESSFGPTPCRAAHFC
                     RACRNAFEGFKVK"
BASE COUNT          775 a         1972 c         2100 g          806 t
ORIGIN      
        1 gcgtacacgc ggaaggtcga gatcgccatc gccacgtccg agaagcggcc cttgcggatc
       61 gtgccgccgg agtcctgggc ctcggtctcc gcgagctcct tgccctgctc cttgatgcgg
      121 ctcgccacgt ccatcaggac ctgggcccgc tcggaaccct tcatccgggg ccaggggccc
      181 tcgtcgaagg cgcgccgcgc ggcctcggcc gcccgctgga cgtcctcggg gccggccttc
      241 gccaccgtgg cgaacggctt gccggtggag gggtcggtgc tctcgaaggt ctcgccggag
      301 gcggcgtcgg cctcctcgcc tgccaggatg agcttgtagg tctccgtcat gacgcggtcc
      361 tcctgggcgc ggatcgggtc gggccactat atcgcggggc cctcgtgcga tgatgccggg
      421 gcatggaccc cttcgtccag gagccgccgc agccgcccga ccggttccga gaggaccggc
      481 ccctgcgcca tgcgctggag cggctcctcc cggccgaggt gttcgaggag gcgctccccg
      541 agctcgccga catggcgggg atcgccacgg gcgagctcca ggagctggcc gaccgcgccg
      601 aggctcaccc tccgcgtcac gtgccctttg acgcctgggg gcggcgggtg gacctcgtgg
      661 aggtgggtcc ggcgtggacc cggctcgtgg agatcgggca ggagcggggc gtcgtggcgc
      721 tcccgtacga ggatcgcttc ggcgagcacg cccgcgtcgt gcagttgggg ctcctctcgc
      781 tctacctccc ggtgtcggca acggccgact gccccctggc gatgaccgac gcggccgtgc
      841 gggtcctcct gaacgaggac ccggagctgg cctcccggta cgtccctcgg atgacctccc
      901 ggcgcgacgc gtggacctcg ggccagtgga tgaccgagac gacgggcggc tctgacgtcg
      961 ggaggaccga gacggtggcc cgggaccaag gggacgggac gtggacgctg cacggtacga
     1021 agtggttcac gtcggccacc accgcagacg tggcgctggc gctggcccgg cccgagggcg
     1081 ccgaggaggg gagccgcggc ctttcgctct tcctcttgga gctgcgggac ggcgaccgct
     1141 ggaacgggat cacggtccgg cggctgaagg acaagcttgg gacgagggcg ctgccgaccg
     1201 ctgagctcga tctcgacggg accgtggcgg tcccggtcgg agggatcggt cggggtgtgg
     1261 ccaagatcgc tgcgatgctc aacgtgacgc ggctgcacgc cgcggcgggg agcctcgggg
     1321 cggccggcta cgggctgtcg ttggcccggg actacgcgta ccggcgcgag gccttcggtc
     1381 ggaagctcgt cgacctcccc gtgcatcgag gatggatcgc gcgcgtggcc gccgagtacg
     1441 aggcgatgaa cgcgctggcg ttccgggccg cggagctgct gggcgcggtg gagcggggcg
     1501 gcggggtccc cgagctggcg cgggtggcga tccccctgac gaagatggcg ctgggccgcc
     1561 agggcgtctg ggccacgagc catttgatcg agtcgttcgg cgggatgggt tacctggagg
     1621 actcgggcct gccccggctc ctccgagacg cccacgtcca gtcgatctgg gaggggacga
     1681 cgtcggtgct ggccctggac gtgcttcggg cgctgggggc gtccggcgtc gcggaagcgt
     1741 tcgcgcagga tgtggcggcg cggacccgcg cggcgaccgg atcgatcctc gaggagcccg
     1801 ccgcgcgggt cctcgccgcc ctggagacgc tccggcccat gctggccgag ccctccgagc
     1861 cccaggcccg ccgcctggcg tgggggatgg cccggaccta ccaggccgcc ctgctgtgcg
     1921 aggcggcggc gtgggcgctg gagacacacg gcgacgaccg cgccgccacg gcgctccggc
     1981 tgttcagctc ggagccactc gtgggcgagg agccgccggc cgaccccgac gcgctgggcc
     2041 gcctcgcctt cgggcgttga cggaagcatc gaccgaggtc aatatgggat cgtgcgagag
     2101 atccccgtga tcgaggagtg ctgcccgccc ctgctccagg cgcccctgga ggaggacgac
     2161 gcggagcgac tggcctcggc cctccgcgtg ctggccgacc cggcccgtct ccgcctggtc
     2221 agcatcatcg gagcgggccc agggagcgag gcctgcgtct gcgacctcac cgagccgatg
     2281 gggctgtccc agcccaccgt cagccatcac ctgaaggtgc tgcacgaggc gggcctgctg
     2341 gagcgggagc gccgcggtcg ctgggtctac taccggctcg tggacggccc gctgcgctcg
     2401 gtggcggggg cgctctcgcc cgccacctcg acgtcgtaag cgtgccaggt ggtcttctcg
     2461 gcccccgggt agcggtcgta gagccgctgg gctcggtggt tgtcggtcgc ggtgaaccac
     2521 tcgaggatcg gaagccctcg ctcgcgcgcg gcctcgacgc acgcgtcgat cagcgcctcg
     2581 ccgatgccgg tgccccgcgc ttcctccacc acgaagaggt cgttcatcag cgcgatgtcg
     2641 ccgggcagcg tcgaggaata ggtccagtag atcgtggcga agcccaccag gcgttcgcca
     2701 tcccacgcgc cgagcagaag cccctcgtcg ctcggcgcca ggagccgacg gaagaacgcc
     2761 cggttgcgct cgtcgtccgg ctcggcctcg tagaagacct gataaccggc gatcagtgga
     2821 agagcgtcct cgagctcctc ctcggccagc gcccgcacct cgaccgccat gcgcgcatcc
     2881 tacgcgtctc ggatggagcc tcgggaggcc gggtagtatg agggcaagga ggacgcaccc
     2941 catgacgagc tacgacgagc gcaccgccga gttcaaggcc cgcatcgacg cgggggagaa
     3001 gatcgaggcc ggcgactgga tgcccgacga gtaccgtcgg tcggccttga agttcatcga
     3061 gatgcacgct aactccgagg tgatgggctg cctgcccgag cgcgagtgga tcccgcgagc
     3121 gccgtctctc cgccgcaagc tttccctgac cgcgaaggtg caggacgagg ccggccacgg
     3181 ccagatcctc taccgcgtgg ccgaggacct cgggaagtcg cgggagcaga tgttcgacga
     3241 tctcgtcaac ggccgcacga agttccacaa cgtcttccac tacccggccg agacctgggg
     3301 cgacgtggcc gtgatcggat ggctgatcga cggcgccgcg ctcgtcacgc agaaggccct
     3361 cctcgactgc tcgtacgccc cctacgtccg ggccatgaag cggatctgcg cagaggagtc
     3421 cctccacctc cgccacggcg aggacctcac gctcgagctc gtctccggca ccgacgagca
     3481 gcgcgagctg ttccaggacg cggtcaaccg ctggtggaag ccgatcatgc acttcttcgg
     3541 cccgccctcg aagccggcca aggacgtcct cctctactgg gggatcaaga cgcggacgaa
     3601 cgaggacctg cgccaggaat tcttctccac ctacgtcccg aagctgtggg acctcgggat
     3661 cagcgtcccc gaccccgagc tccgctacga cgaggaggcg ggcgagtgga tctggtccga
     3721 ccccgactgg gacgagttct ggcaggtggt caagggcaac ggcccgatga cccagacgag
     3781 gctcttctgg cggaaggcga tctgggacca ccacgcctgg gtgcgggaga cgttcgacag
     3841 gatccccgcg gccgcggtgt gacgcgatga tcgtgtggga cgtcctccag cgccgccgcg
     3901 ccggcgccga ctgggaggtg gtcggcgggg tgaaggcgcc ggacgccgac ttcgccatcc
     3961 tgctggcccg cgagtccctc ttccggcgtc gcgagggtga gacctacgcg gttcgccgcc
     4021 gcggcaccga ggatctgatc gagatcacgg acccgatcgg gatcggcggc gtgatcgacc
     4081 gggagttccg caggatggac tcgttcgccg gggtcggcgg caagcaccgc aggatccacg
     4141 aggagatgcg ggagcgcggc ctcgtgatcg accgcccgcg gcctcccgac cgcaggcagc
     4201 gtcgctcggc gaagcagggt ccatctgagg agaccgcccg tgcctgaggg cgaccctcgt
     4261 ctgatcctgc tgctcgcgct ggccgacgac gagctgatca tcgggcaccg ccactccgag
     4321 tggactggct gggctccgca cctcgaggag gacctggcct tcagctcgat cgcccaggac
     4381 gagatcgccc acgctagggt gctgctcgag ctggccgagc ccctggccgg ccgcggggtg
     4441 gacgagctcg cgctcggccg ctcgccgggc gagtaccgcc acgccgtggt ctgcgagcgg
     4501 cccaacgggc actggggcta cacggtggcg cgccagtacc tctacgacac ggccgacgat
     4561 gtgcggctcg ccgcgctcga ggggtcgtcc gacgccgacc tggccgccgc cgtcaagacg
     4621 atccgcctcg aggagcgcta ccacgtggag cacgcccgga tctggttccg ccgcctggcc
     4681 gagggcccgg tccaggcccg ggagcatctg tccgaggggt tggagcaggc cgtgcccgag
     4741 gccctggccc tgttcgagga gctgccgggc gaggaggccc tcgtcgcgga tggcgtcctc
     4801 ccctcggcca tggaggacct gctgtccacc tggctcgagg ggctcggggc cgacctcgag
     4861 tcggtctcgc tcgactacgt gctctcccgc cacaagaggg tgggggagct ggtcccgacc
     4921 tcctcgggcg aggcccagga ggggtcgacg ctcgaggtgc cggggctgga gcgccgcggc
     4981 gatcgctggg tggccgtcgg aggcttcgcc ggcgccggag ggcgccgggg aaagcacacc
     5041 gaggacttcg acgagctgtg ggaggagatg acgatgctgt accggaaggt cccgggggcg
     5101 acttggtgac gccgatcgcc gccgccgacg tccgggaccg ggtccgccgc gctctggagc
     5161 gcgtgatgga cccggagatc cccaccgcct cgatcgtgga tctggggatg gtcgagcgcg
     5221 tcgaggtgcg cgacgacctc gtcgaggtag acctcctgcc cacgttcgtc ggctgcccgg
     5281 ccaaggacgt gatcggggag gacgccgagg ccgccgtccg cgaggccgcc ggggaccggg
     5341 acgtgcgcgt gcggttcgtc ttcgacccgc cctggacgac cgaccggatc accgagaagg
     5401 gcaaggccgc gctccgcgag tacggcatct cgccgcactg ggagggcggt ccccccgacc
     5461 ggggcgccgt ggtcgtcccc ctgctggcga agcccgcggg gatcccgtgc ccctactgcg
     5521 gctcggaccg gacggtcacc gagtcctcgt tcggtcccac gccctgccgc gcggcccact
     5581 tctgccgcgc gtgccgcaac gccttcgagg gcttcaaggt gaagtgagag gcgcccgccg
     5641 ctccgcggcg ggc
//
LOCUS       DASUTZ010000155         4984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_565235, whole genome shotgun sequence.
ACCESSION   DASUTZ010000155 DASUTZ010000000
VERSION     DASUTZ010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4984
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565235"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VF097_06790"
     CDS             <1..561
                     /locus_tag="VF097_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HEX6262537.1"
                     /translation="AGRKKDYRWRLLGPERAGRAGMRRLGIGALLGLHDDWRYEAIAT
                     AAHARFLQRHAWRSQVTVSVPRIRPSASGFQPRVDVSDHDLTQLVCALRLGLPDAGIV
                     LSTREPPELRDGLHRIGITHTSAGSHTEPGGYEHPGEAQEQFEIADERTPAEVAASLR
                     AAGYDVVWEDPATPEGIGRGLAAVRG"
     gene            599..1279
                     /locus_tag="VF097_06795"
     CDS             599..1279
                     /locus_tag="VF097_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6262538.1"
                     /translation="MDPRRDIERIALLREPVRRRLYDIVRGSGRAVSREEAAAEAGVS
                     RSLAAFHLDRLADAGLLEVEYRRLSGRRGRGAGRPAKLYRPGADRVEVSFPPTRYALA
                     GDLMIRALRQGGGGRPPVESARASSEAYGREAARELGRGIRRRAPLSRMGEALERLGF
                     EPENDGERVQLRNCPFHELAQRDPELMCAMNQAFLRGLAEGLTGGRAAVRARVEDGYC
                     CAVIEKAP"
     gene            1276..2103
                     /gene="thiE"
                     /locus_tag="VF097_06800"
     CDS             1276..2103
                     /gene="thiE"
                     /locus_tag="VF097_06800"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014141984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX6262539.1"
                     /translation="MIRVTANGRALQVDDDSSVADLIRSRDLDPRFVVVELNGEPLER
                     SRYEEVTLSDGDRLELVRAVAGGADLRARLEASRLYVVADARREQGDLEVFLDAILGA
                     GVDIVQLREKEAEAGDLLRWAETFRAAADRHGALFTINDRPDVAIAAGADGVHVGQND
                     LPVGVARRVVGDDLIVGLSTHAAGEWDAAPSEADYLTAGPVWETPTKPGRPAAGLEHV
                     AHAAGHADRPWFAIGGITPENLPQVVEAGATRIVVVRAVTEADDPAAGVERLLRLLP"
     gene            2170..2382
                     /locus_tag="VF097_06805"
     CDS             2170..2382
                     /locus_tag="VF097_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262540.1"
                     /translation="MAIDERSRHRMYERLQEVLGEEEAATLMEHLPPVGWADVVTKHD
                     LDLRLEALEQRLLATFREELTDRPGR"
     gene            2550..2927
                     /locus_tag="VF097_06810"
     CDS             2550..2927
                     /locus_tag="VF097_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262541.1"
                     /translation="MTIDERSRHRMYERLQEILGDEEAATLMEHLPPVGWADVATKRD
                     IDALEVATRRDLDALKAATRRDLDALKAATKRDLDVLKNELLATFRAELNEQTRTMIH
                     AMIFSTSAAVLTVGSLAVAAVRL"
     gene            complement(3221..4303)
                     /locus_tag="VF097_06815"
     CDS             complement(3221..4303)
                     /locus_tag="VF097_06815"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6262542.1"
                     /translation="MGAAGARARPQRLTALRILAAAFAAGLLGSAIWRFRRRRITRLN
                     LLISSAIALALGLLSVAPGLFDPVFDFFDFQRGGGQRLLAVLIFGNIVLLLLVLRTMS
                     ETDQVRRDFKVLMEWMGRQTFDQERAEGLPAGDRIVVVLPAFNEADSVGEVLDQMPEK
                     IDGHPVVPLVVDDASEDATAGVARAHGALVTSLPIRRGGGQAMRVGYELALRLGAVVV
                     ASLDADGQHLPQELPDVVGPILRDEADMVQGSRMLGSYQKESRIRHLGVLFFSGLVSL
                     MTGTRLTDVSNGYRATRADTLRTFFLDEDQFWTSEILIEGLRHRARIVEVPITVLARK
                     HGQTKKPRPLKYGWSFLKVIMKTWLR"
     gene            complement(4264..>4984)
                     /locus_tag="VF097_06820"
     CDS             complement(4264..>4984)
                     /locus_tag="VF097_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015202191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
                     /protein_id="HEX6262543.1"
                     /translation="DIEEGFFEVHHKGRSDTVPFPKQPGSLYHASKVHDSTNIHFACR
                     AWGLSATDLNQGIVYGIETPETRLDPRLATRFDYDETFGTALNRFCVQAVIGLPLTVY
                     GAGGQTRGYLNIVDTLQCVELAVLHRAKPGEYRVFNQFTESFSLMELAETVARAGRDV
                     GIEVRIDHLENPRVEAEQHYYNPAHTKLLDLGLEPHYLSETLIESMFEAIRRHEDRVV
                     RDHILPETRWAPRAPERAPSG"
BASE COUNT          731 a         1738 c         1792 g          723 t
ORIGIN      
        1 gccggtcgga agaaggacta tcgctggcgg ctgctggggc ccgagcgggc gggccgggcc
       61 gggatgcgcc gcctggggat cggggcgctg ctgggcctcc acgacgactg gcggtacgag
      121 gcgatcgcca ccgccgccca cgcccgcttc ctccagaggc acgcctggcg gagccaggta
      181 acggtctcgg tgcccaggat ccgcccgtcg gccagcggct tccagccccg ggtggacgtc
      241 tcggaccacg acctcacgca gctcgtgtgc gccctccggc tcgggctgcc cgacgccggg
      301 atcgtcctct ccacccgcga gccgccggag ctgcgggacg ggctgcatcg gatcggcatc
      361 acccacacca gcgccggatc gcacacggag cccggcggct acgagcatcc cggcgaggcc
      421 caggagcagt tcgagatcgc cgacgagcgc acgccggccg aggtcgcggc gtcgctccga
      481 gccgcgggct acgacgtggt gtgggaggac ccggccaccc ccgaggggat cggccggggc
      541 ctggcggccg tccggggctg agcccccgta tcaccaacgg ctctttacaa tggaggcgat
      601 ggatccccgc cgggatatcg agcgcatcgc cctcctccga gagcccgttc gccgccgtct
      661 gtacgacatc gtgcggggat ccgggcgagc cgtcagccgg gaggaggccg ccgcggaggc
      721 cggcgtctcg aggagcctgg cggcgttcca cctggaccgc ctggcggacg ccggcctgct
      781 cgaggtcgag taccgacgcc tgtcgggacg ccgcggccgc ggagcggggc gccccgcgaa
      841 gctgtaccgg ccgggggccg atcgcgtcga ggtgagcttc ccgccgaccc gctacgcgct
      901 ggccggcgac ctcatgatcc gtgctcttcg ccaggggggc ggcggccgac cacccgtcga
      961 gtcggcccgg gcctcctccg aggcctacgg tcgcgaggcg gcccgggagc tgggccgcgg
     1021 gatccggcgc cgcgcgccgc tctcgcggat gggggaggcg ctggagcgcc tcgggttcga
     1081 gcccgagaac gacggcgagc gtgtgcagct gcggaactgc ccgttccacg agctggcgca
     1141 gcgggatccc gagctgatgt gcgccatgaa ccaggcgttc ctccggggcc tggccgaggg
     1201 cctgacggga gggcgggccg cagtgcgggc tcgcgtcgag gacgggtact gctgcgcagt
     1261 gatcgagaag gccccgtgat ccgcgtcacg gcgaacggga gggctctcca ggtcgacgat
     1321 gactcctcgg tggccgacct gatccggtcg cgagacctcg atccccggtt cgtcgtcgtc
     1381 gagctgaacg gcgagccgct ggagcggtcg cggtacgagg aggtgacgct ctccgacggc
     1441 gaccggctgg agctcgtccg ggcggtggcg gggggagcgg atctccgggc ccggctcgag
     1501 gcgtcccgcc tctacgtcgt cgccgacgcc cggcgggaac agggcgatct cgaggtcttc
     1561 ctcgacgcga tcctcggggc cggcgtggac atcgtccagc tccgcgagaa ggaggccgag
     1621 gcgggcgacc tgctgcgctg ggccgagacg ttccgcgccg cggccgaccg gcatggagcg
     1681 ctcttcacga tcaacgatcg ccccgacgtg gcgatcgcgg cgggcgccga cggcgtccac
     1741 gtcggccaga acgacctccc ggtcggcgtg gcccgccgcg tggtcgggga cgacctcatc
     1801 gtcgggctgt ccacgcacgc ggcgggggag tgggacgcgg ccccgtccga ggccgactac
     1861 ctgacggccg gcccggtctg ggagacgccc acgaagcccg gccgcccggc ggcaggcctg
     1921 gagcacgtgg cccacgcggc gggccacgcc gaccgcccgt ggtttgccat cggcgggatc
     1981 acgccggaga acctgcccca ggtggtggag gccggcgcga cccgtatcgt cgtcgtgcgc
     2041 gccgtgaccg aggccgacga cccggccgcc ggggtcgagc gactccttcg actgctcccc
     2101 tgatcgtgtc ggtgcccacc ggtagccttc ctgcatcccc tcttgtcgct tcacggaggg
     2161 aggaccacaa tggcgatcga tgagcgctca cgtcatcgca tgtacgagcg cctccaagag
     2221 gtgctcggcg aggaggaggc ggccacgctg atggagcacc tcccgcccgt gggctgggcc
     2281 gacgtggtca ccaagcacga cctcgacctg cgcctcgagg ccctcgaaca gcggctgctg
     2341 gcgaccttcc gcgaagagct cacagacaga cccggacgat gatccacgcg atgatcttct
     2401 ccacctcggc ggcggtcctg accgtgggca gcctcgcggt ggccgcggtc cgcctctaac
     2461 cggtctcgcg ctcccgccct gatcgtgtcg gtaaccaccg gtagtcttcc cgcatccccc
     2521 tcttgtcgct tcaccgaggg aggaccacaa tgacgatcga tgagcggtcg cgccaccgga
     2581 tgtacgagcg ccttcaagag atccttggcg acgaggaggc tgccacgttg atggagcatc
     2641 tcccgcccgt ggggtgggcc gacgtggcga cgaagcgcga catcgatgct ctggaggtcg
     2701 ccacgaggcg ggatctggat gctctgaagg ccgcgacgag gcgggatctg gatgctctga
     2761 aggccgcgac gaagcgggac ctggacgtcc tgaagaacga gctgctcgcg accttccggg
     2821 ccgagctcaa cgagcagacg cgaacgatga tccacgcgat gatcttctcg acctcggccg
     2881 ccgtcttgac ggtgggcagc ctcgcagtgg ccgcggtacg actctagccg gacgagagga
     2941 gcccgatcat cccgtagacc agaagcacga cggtgatggc cccgatcagc agaaggccta
     3001 cgatcacgag gggtctgctg ctggagtggt ccctggcacg atgaggtcgg atcccgccgc
     3061 ttgcgggcca ccggatctcg gagttggtgt aagagctcca cctggcgtcc tcgtggaagc
     3121 ggctcaccag gtctcgctct cgccgcccta tctttcccat ctcttcttct ttcggccgcc
     3181 ctgcttgtct tgagcctacc gtgcgaaccg cgacaacgcc tcaacggagc caggtcttca
     3241 tgatcacctt gaggaagctc cacccgtact tcagcggccg cggcttcttc gtctggccgt
     3301 gcttccgggc caggacggtg atcggcacct cgacgatccg ggcccggtgc cgcagcccct
     3361 cgatcaggat ctccgacgtc cagaactgat cctcgtcgag gaagaacgtg cgcagggtgt
     3421 ccgcacgcgt ggcccggtac ccgttggaga cgtcggtaag gcgggtgccg gtcatcaggg
     3481 agacgaggcc cgagaagaag agcacgccca ggtgccggat gcgactttcc ttctggtagg
     3541 agccgagcat ccgcgagccc tgaaccatgt cggcctcgtc tcggaggatc ggtcccacca
     3601 cgtcgggcag ctcctggggc aggtgctggc cgtcggcgtc gagcgacgcg accacgaccg
     3661 cgcccagccg cagcgccagc tcgtagccca cgcgcatcgc ctggccgccc ccgcgccgga
     3721 tcgggaggct cgtcaccagc gccccgtggg cgcgagcgac ccccgctgtc gcgtcctcgg
     3781 aggcgtcgtc cacgaccagc ggcacgaccg ggtggccgtc gatcttctca ggcatctggt
     3841 ccaggacctc gcccacgctg tcggcctcgt tgaacgcggg gaggacgacg acgatccggt
     3901 cgccggccgg gaggccctcc gcccgctcct ggtcgaaggt ctgccggccc atccactcca
     3961 tcaggacctt gaagtcgcgc cgcacctgat ccgtctcact catcgtccgg aggacgagca
     4021 gcagcaggac gatgttcccg aagatcagga ccgccaacag gcgctggccg ccgcctcgct
     4081 ggaagtcgaa gaagtcgaag accgggtcga acagcccggg ggccacggag aggagcccca
     4141 gcgcgagggc gatggcagac gagatcagca ggttgagccg cgtgatccgt cggcggcgga
     4201 agcgccagat cgcggagccg aggagcccgg cggcgaaggc cgccgcgagg atccggagcg
     4261 cggtcaaccg ctgggggcgc gctcgggcgc ccgcggcgcc caccgggtct cggggaggat
     4321 gtggtcccgg accactcggt cctcgtggcg ccggatcgcc tcgaacatcg actcgatcag
     4381 ggtctcggag aggtagtggg gctcgagccc caggtcgagg agcttggtgt gggccgggtt
     4441 gtagtagtgc tgctcggcct ctacccgggg gttctcgagg tggtcgatcc tcacttcgat
     4501 ccccacgtcg cgcccggccc tggccaccgt ctcggccagc tccatcagcg agaaggactc
     4561 cgtgaactgg ttgaagaccc ggtactcgcc cggcttggcc cggtggagga ccgccagctc
     4621 gacgcactgc agggtgtcca cgatgttgag gtacccacgc gtctgcccgc cggcgccgta
     4681 caccgtcagg gggaggccga tcacggcctg gacgcagaag cggttgagcg ccgtcccgaa
     4741 cgtctcgtcg tagtcgaagc gggtggccag gcggggatcg agccgcgtct ccggggtctc
     4801 gatcccgtag acgatcccct ggttgaggtc ggtcgcgctc agcccccacg cccggcacgc
     4861 gaagtggatg ttcgtcgagt cgtgcacctt cgaggcgtgg tagagcgacc cgggctgctt
     4921 ggggaacggc acggtgtcgc tccgtccctt gtggtggacc tcgaagaagc cctcctcgat
     4981 gtcg
//
LOCUS       DASUTZ010000156         2301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_46626, whole genome shotgun sequence.
ACCESSION   DASUTZ010000156 DASUTZ010000000
VERSION     DASUTZ010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46626"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..864
                     /locus_tag="VF097_06825"
     CDS             106..864
                     /locus_tag="VF097_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262544.1"
                     /translation="MPDYQGRLDQIERAVDEGESDLRALGFWSLVSEAKRDPGAAGHA
                     EQIGRIDRRAFEARKRPLFPVWLGNLVLLLGILVLLAAAAVALRLASGSLGGEPRSEI
                     AGVVLVGAGLGLSAAVHAPAHWLLGRIVGMRFTRYFLGGPFRIQPGVKVDYGSYLLTP
                     ASQRATMHAAGALASKVAPFVVFGWAYVYHWQRGWDLLPPWSLWALLGLGLFQIVTDL
                     LWSTRRSDWKKVRRELRIAREVEGTGEPADAGIG"
     regulatory      878..963
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1012..1818
                     /locus_tag="VF097_06830"
     CDS             1012..1818
                     /locus_tag="VF097_06830"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEX6262545.1"
                     /translation="MEDQLVIAGRPFRSRLIVGTGKFGSHEVMRESLEASGAEMVTVA
                     LRRVDLDASGGPDILEFIDPERYLVLPNTSGAADADEAVRLARLARAAGLPEWVKLEV
                     TPEPRYLLPDPVETLTAAEKLVADGFTVLPYIGADPVLAKRLEETGCATVMPLGSWIG
                     SNQGLRTRDAVGIIVEQATVPVVVDAGLGAPSHAADCMEMGVDAVLVNTAIAVAGDPP
                     AMARAFAKAVEAGREAWRARLGEVRAEAEASSPLTGFLERPTEPDPLPEA"
     gene            1829..>2301
                     /locus_tag="VF097_06835"
     CDS             1829..>2301
                     /locus_tag="VF097_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013423000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262546.1"
                     /translation="MTSLADLAEPRPERDGFLDPYPSRTFAQELVVLPMDDLLGVAHG
                     APASRVDHALAARDRTLEDLAALISPAAGERLEELAVASRALTLARFGRTMHLYAPVY
                     LSNECLTTCTYCGFARNLPVERKTLREEESVDEARHLVEQGYRSLLLLTGEHERNT"
BASE COUNT          293 a          802 c          873 g          333 t
ORIGIN      
        1 gcccatgccg ccggcggcga ccctggcgag gatctggtag cgtccgtcta cggtcgtctc
       61 gatcatctcg gggggagcgg cctcgtctcc gcattgtagg gggtcatgcc cgactaccag
      121 ggaaggctcg accagatcga acgggccgtg gacgagggcg agagcgacct ccgcgcgctc
      181 gggttctgga gcctggtctc ggaggcgaag cgcgacccgg gcgccgcggg ccatgcggag
      241 cagataggga ggatcgatcg gagggcgttc gaggcgcgga agcggccgct gttccccgtg
      301 tggctaggca atctcgtgct cctcctgggg atcctggtcc tgctcgccgc cgccgcggtg
      361 gctctgcggc tcgcctcggg aagcctcgga ggggagcccc ggtcggagat cgccggcgtc
      421 gtcctcgtcg gcgcgggcct cgggctctcg gccgccgtgc acgccccggc ccactggcta
      481 ctcgggcgca tcgtggggat gcgattcacg cgttacttcc tgggcggccc cttccggatc
      541 cagcccgggg tgaaggtcga ctacgggagc tacctcctca ccccggcatc gcagcgggcc
      601 acgatgcacg ccgcgggggc cctggcgtcg aaggtcgcgc cgttcgtggt cttcggctgg
      661 gcctacgtct accactggca gcgggggtgg gacctcctcc cgccctggtc gctgtgggca
      721 ctgctcgggc tcgggctctt ccagatcgtc accgacctgc tctggtcgac gaggagatcc
      781 gactggaaga aggtgcggcg ggagctacgc atcgcccggg aggtcgaggg gaccggcgag
      841 cccgccgacg ccgggattgg gtaggatggg agcgaaccgg ggagcccgga gggaccgggc
      901 tgagaggccg gctgcaccgt cggcgacccg cggaacctga tccgggtaat gccggcgcag
      961 ggaggcgcgg gccgccggtc gtccggcggc cgttcccgtt ccggaggagg catggaggac
     1021 cagctcgtca tcgcgggccg tccgttccgg tcccggctga tcgtgggcac cgggaagttc
     1081 gggtcgcacg aggtcatgcg ggagtcgctc gaggcgtcgg gcgccgagat ggtgacggtg
     1141 gctctgcggc gcgtggacct ggacgcgagc ggcggccccg acatcctcga gttcatcgac
     1201 cccgagcgct acctcgtgct ccccaacacc tccggggccg ccgacgccga cgaggcggtt
     1261 cggctggcgc ggctggcccg ggccgccggg ctcccggagt gggtgaagct cgaggtcacc
     1321 cccgagcccc ggtacctgct tcccgatccc gtggagacgc tgaccgccgc ggagaagctc
     1381 gtggccgacg gcttcaccgt cctgccgtac atcggggccg acccggtact ggcgaagcgc
     1441 ctcgaggaga ccggctgcgc cacggtgatg ccgcttggct cgtggatcgg gtcgaaccag
     1501 ggcctgcgga cgcgcgacgc ggtcgggatc atcgtggagc aggccacggt cccggtcgtc
     1561 gtggacgcgg gcctcggcgc gccctcgcac gccgccgact gcatggagat gggcgtggac
     1621 gcggtcctcg tcaacacggc gatcgcggtg gcaggtgatc ccccggcgat ggctcgggcc
     1681 ttcgccaagg cggtggaggc gggccgcgag gcgtggcggg cgcggctcgg cgaggtccgc
     1741 gccgaggccg aggcctccag cccgctcacc ggattcctgg agcggccgac ggagcccgac
     1801 cccctcccgg aggcctgacg tgtcgcggat gaccagtctc gcggacctcg ccgagccgcg
     1861 ccccgagcgg gacggcttcc tcgacccgta cccgtctcgc accttcgccc aggagctcgt
     1921 cgtcctgccg atggacgacc tgcttggggt ggcccacggc gcccctgcgt cccgcgtgga
     1981 tcacgcgctg gccgcccgtg accgcaccct cgaggatctc gccgccctga tctccccggc
     2041 cgcgggagag cgcctggagg agctggcggt ggcctcgcgg gccctgacgc tggcccgctt
     2101 cggccggacg atgcatctct acgcgccggt ctacctctcg aacgagtgcc tgacgacgtg
     2161 cacgtactgc ggcttcgcca ggaaccttcc ggtggagcgg aagacgctcc gcgaagagga
     2221 gagcgtggac gaggcccggc acctggtcga gcagggctac cggtccctgc tgctgctgac
     2281 cggcgagcac gagcggaaca c
//
LOCUS       DASUTZ010000157         6747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1082692, whole genome shotgun sequence.
ACCESSION   DASUTZ010000157 DASUTZ010000000
VERSION     DASUTZ010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6747
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082692"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2569)
                     /gene="aceE"
                     /locus_tag="VF097_06840"
     CDS             complement(<1..2569)
                     /gene="aceE"
                     /locus_tag="VF097_06840"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEX6262547.1"
                     /translation="MMEPFKYQLPDADPQETAEWLEALRQLMEDSPARARFLIYQLTR
                     EAQARHLGVPSMVSTPYINTIPPEQEPEFPGDEDMERRIRRLIRWNAAVMVSRANKRY
                     EGIGGHISTYASGASLVEVGFNHFFRGRSHPGGGDHVYFQGHGAPGIYARAFLEGRLT
                     EDELEAFRREAFGEGLSSYPHPRLMPEFWEFPTVSMGLSPIAAVYQARFDRYLLNRGI
                     KDTSDQRVWAFVGDGEMDEPESLAGLTIAAREGLDNLIFVVNCNLQRLDGPVRGNGKV
                     IQELEALFRGAGWHVIKVIWGREWDDLLARDSDGVLVAKMNQTVDGEFQRYAVDDGAY
                     IRERFFGPDPRLQELVAHLSDEDLRRLRRGGHDYSKLYAAYKRATELTGAPVVILAKT
                     VKGWTIQGLEARNVTHQQKKLAAQELKVFRDRLELPIEDDEIHEGEAPYMTAKRFPEE
                     AEYLRERRSELGGFVPKRRAAAPGLTLPGDDVYAEFDAGSEQPVATTMAMVRLIRKLM
                     RDDRVGERVVPIIPDEARTFGMESLFPEFKIYAPHGQIYEPVDAAHVLAYKEAKDGQV
                     LEEGITEAGSMASFQAAGTSYATHGEPMIPFFIFYSMFGFQRIGDLIWQAADMRARGF
                     LLGATAGRTTLNGEGLQHEDGHSLLLASTNPAVLAYEPTFAYEVAAIVRDGLRRMQDE
                     GEDLIYYLTLQNEPYPQPKRPDGLEDAEILKGMYRFRATDGERRFRAQILGSGSIMQE
                     ALRAQELLAEHHDVAADVWSATSYHAMRLEAIEVERWNRLHPGEAPRAPFVTRQLEGA
                     DGPVIAVSDWMRAVPDQIARWVPGPYRSLGTDGFGRSDTRPALRRFYEVDAEHVVVAT
                     "
     gene            complement(2629..3111)
                     /locus_tag="VF097_06845"
     CDS             complement(2629..3111)
                     /locus_tag="VF097_06845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX6262548.1"
                     /translation="MRRLRMQRDREGGFTLVELMVVLLIIAILIAIAIPTFLEYRRRA
                     SNRAVQAHLSTVLKAQRGFMADPPFVFTSDGNDLEAIEPGLNYDTAANPTCAAYVDQY
                     CVRVEVLGPDEVLLIARSGTGEYWAIRDVTVGPGAGTFYNQGGDAAPPAAAAVVDDSW
                     "
     gene            complement(3156..3227)
                     /locus_tag="VF097_06850"
     tRNA            complement(3156..3227)
                     /locus_tag="VF097_06850"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3193..3195),aa:Val,seq:cac)
     gene            3435..4154
                     /locus_tag="VF097_06855"
     CDS             3435..4154
                     /locus_tag="VF097_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEX6262549.1"
                     /translation="MSDDIGRKMLAEFVGTFALIFVGAGSIVAASVIGGGDLAPNLVT
                     IALAHGLVIAVMVSAIGHVSGGHLNPAVTIGAWTAGKIRTGDAAAYLVTQLAAGVAGA
                     LLLRAVVPEDAWRSVSLGATLLGDVTVAQGIAIEAVLTFLLVWVIFAVAVDPDGTFRA
                     VAGLPIGLTVALDIMMGGPFTGASMNPARSFGPAVAGGFWTDHWVYWIGPVAGGVLAA
                     VLYDLLVLRRRRPEPTLEEAP"
     gene            4159..5223
                     /locus_tag="VF097_06860"
     CDS             4159..5223
                     /locus_tag="VF097_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007029492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX6262550.1"
                     /translation="MSTPPEMLEALRLLVEAESFSSDPGGLERCGLVLRDLCRGILGS
                     PPEAEGHVLRWERPGDRPILILCHYDTVWPTDTLAHFPFEVRDGVARGPGAFDMKAGI
                     VQGLFAIRDSSGPVTMLLTHDEERGSAESQEAIEREALTSRAVLVLEPSADGGAVKTA
                     RKGVAHWRIEVKGRAAHAGLEPEKGVNAAIEIARQLERISSFGDPAAGTTVTVTMVRA
                     GSAENVVPEEAWASVDARVWTREEGERLERAFGALEPLTEGAHLSVEGGMNRGPLGRE
                     ASGDLYQRLRRLGYDLPAAEVGGGSDGNFTAALGVPTLDGLGAEGGGAHARNEHVLVD
                     AMPWRAEMVARLIDDLAADG"
     gene            5216..6001
                     /locus_tag="VF097_06865"
     CDS             5216..6001
                     /locus_tag="VF097_06865"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6262551.1"
                     /translation="MAEVEIRDLAEIDDLRSTLELFERIWGARERMFAVEVLRAVATH
                     GGAILGAFLDGRMVGAQVAFLGRDENELLLHSHVTGVEPTLQHEGIGSALKWAQRDWA
                     LERGIELITWTFDPMISRNAYLNLRKLGASARRFLRDFYGRMDDDINVGERSDRLEIT
                     WRLKDPRVEGAAAGGPVARDAEGAAVLLDAPEGRPRPHPEALGAPLVLARVPTDYLDL
                     RARDPEVARAWRDAAADVLERALSGGYAAVDFLREGAYVLERS"
     gene            5998..>6747
                     /locus_tag="VF097_06870"
     CDS             5998..>6747
                     /locus_tag="VF097_06870"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX6262552.1"
                     /translation="MSLEAIELILIRLPLLRPFTTSFGTLTDKEAILIRAVDEDGLEG
                     WGECAVAEDPLYSEEWNPGAWMILRNYLAPAALEGASSGIKGHPMARTAMEVALLDLR
                     LRQKQVSLASHLGAVRDRIVCGVSLGIEESIDELLKQVDRFVADGYRRIKLKIAPGWD
                     LEPVGAVREAFPDTALSVDANAAYGLEDADHLARLDAFDLDYLEQPLPKDHLLAHADL
                     QQRIETPICLDETLTSPHVTDEAIRLEACRVV"
BASE COUNT          943 a         2363 c         2432 g         1009 t
ORIGIN      
        1 gcgtggccac gacgacgtgc tccgcgtcca cctcgtagaa gcggcgcagc gccggcctgg
       61 tgtcggagcg tccgaacccg tcggtcccga gcgagcggta gggccccggc acccaccgcg
      121 cgatctggtc ggggacggcc cgcatccagt ccgacaccgc gatcaccggc ccgtcggccc
      181 cctcgagctg ccgggtgacg aacggagccc ggggcgcctc gcccgggtgg agccggttcc
      241 agcgctcgac ctcgatcgcc tcgagccgca tcgcgtggta cgaggtcgcg ctccagacgt
      301 cggctgccac gtcgtggtgc tcggccagca gctcctgagc ccgcagggcc tcctgcatga
      361 tcgaacccga ccccaggatc tgggctcgga agcggcgttc cccgtccgtg gcgcggaacc
      421 ggtacatccc tttgaggatc tccgcgtcct ccaggccgtc gggccgcttc ggctgcgggt
      481 agggctcgtt ctggagcgtg aggtagtaga tgaggtcctc gccctcgtcc tgcatcctgc
      541 gcaggccgtc gcggacgatc gccgcgacct cgtaggcgaa cgtcggctcg tacgccagca
      601 ctgcggggtt cgtggaggcc agcagcagcg aatggccgtc ctcgtgctgg agaccctccc
      661 cgttcagcgt cgtgcggccg gccgtggcgc ccaagaggaa gccccgggcc cgcatgtctg
      721 ccgcctgcca gatcaggtcg ccgatccgct ggaacccgaa catcgagtag aagatgaaga
      781 aggggatcat cggctcgccg tgcgtcgcgt aggaggtgcc ggcggcctgg aaggaggcca
      841 tcgagccggc ctcagtgatc ccctcctcca ggacctgtcc gtccttggcc tccttgtagg
      901 ccaggacgtg cgccgcgtcg accggctcgt agatctgccc gtgcggggcg tagatcttga
      961 actccgggaa gagcgactcc ataccgaagg tccgggcctc gtcggggatg atcgggacca
     1021 cccgctcgcc cacccgatcg tcccgcatca gcttcctgat gagccggacc atcgccatcg
     1081 tcgtggccac gggctgctcc gaccccgcat cgaactcggc gtagacgtcg tcgccgggga
     1141 gggtcagtcc cggggccgcc gcgcgacgct tcgggacgaa gccgccgagc tcgctccgac
     1201 gctcccggag atactcggcc tcctcgggga agcgctttgc ggtcatgtac ggcgcttcgc
     1261 cctcgtggat ctcgtcgtcc tcgatcggaa gctccagccg gtcgcggaag accttcagct
     1321 cctgggccgc gagcttcttc tgctggtggg tgacgttcct ggcctcgagg ccctggatcg
     1381 tccagccctt cacggtcttg gccaggatca ccaccggcgc cccggtgagc tcggtggccc
     1441 gcttgtaggc ggcgtagagc ttcgagtagt cgtggccacc gcggcgcagg cggcgcaggt
     1501 cctcgtcgga gaggtgggcc accagctcct ggagcctggg gtcggggccg aagaagcgct
     1561 cgcggatgta cgcgccgtcg tccaccgcgt accgctggaa ctctccgtcc acggtctggt
     1621 tcatcttcgc caccagaacg ccgtcgctgt cccgagccag gaggtcgtcc cactcgcggc
     1681 cccagatcac cttgatcacg tgccatccgg cgccgcggaa cagggcctcg agctcctgga
     1741 tcaccttccc gttcccgcgg accgggccgt cgaggcgctg gaggttgcag ttcacgacga
     1801 agatcaggtt gtccaggccc tcgcgggcgg cgatcgtcag gccggccagc gattcgggct
     1861 cgtccatctc gccgtccccg acgaacgccc acacccgctg gtcggacgtg tccttgatcc
     1921 cccggttcag caggtagcga tcgaagcggg cctgatacac ggccgcgatc gggctgagtc
     1981 ccatcgagac cgtggggaac tcccagaact cgggcatgag ccgcgggtgg gggtaggacg
     2041 agaggccctc accgaacgcc tcgcgacgga aggcctcaag ctcgtcctcg gtcaaacgcc
     2101 cctccaggaa ggcccgagcg tagatcccgg gcgcaccgtg gccctggaag tacacgtggt
     2161 ccccgccgcc cggatggctc ctgccccgga aaaaatggtt gaagccgacc tcgaccaggg
     2221 aggcgcccga ggcgtacgtc gagatgtgcc ccccgatccc ctcgtagcgc ttgttggcgc
     2281 gggacaccat gaccgcggcg ttccagcgga tcagtcggcg gatgcgccgc tccatgtcct
     2341 cgtcgccggg gaactccggc tcctgctccg gggggatcgt gttgatgtag ggggtggaga
     2401 ccatcgacgg gacgccgagg tggcgagcct gcgcctcccg cgtgagctgg tagataagga
     2461 agcgcgcgcg ggccggcgag tcctccatga gctgccggag ggcctccagc cactccgcgg
     2521 tctcctgcgg atccgcgtcg ggaagctggt acttgaacgg ctccatcatc ggttcgccac
     2581 ccctttcccc accgatcgta cccaacggat gggtgaggcg aggcgcggct accaggaatc
     2641 gtctacgacg gcggccgcgg cgggcggcgc ggcgtccccg ccctggttgt agaaggtgcc
     2701 cgcgcccggg ccgacggtca cgtcccggat cgcccagtac tcgcccgtgc cggagcgagc
     2761 gatcaggagg acctcgtccg ggcccagcac ctcgacccgc acgcagtact ggtccacgta
     2821 ggcggcgcag gtcgggttgg ccgccgtgtc atagttaagg cccggctcga tcgcctccag
     2881 gtcgtttccg tcgctcgtga agacgaacgg gggatccgcc atgaagcctc gctgagcctt
     2941 gaggaccgtg ctcaggtggg cctggaccgc gcggttcgac gcccgccgtc ggtactcgag
     3001 gaacgtgggg atcgcgatcg cgatgaggat cgcgatgatg agcaggacca ccatgagctc
     3061 cacgagcgtg aagcccccct cccggtcccg ttgcatgcgc agccgccgca tttcatagaa
     3121 gtgtcggcac gaggagggcg tccttgagcg cagggtgggc gcggcaggat ttgaacctac
     3181 gacctctcgc gtgtgaggcg agcgctctcc cgctgagcta cgcgccctgg ccgctccgag
     3241 gatagcaccc gcgagctccc ggaaaggtga cggcggggcg tgcccgcgtc gccccgccgc
     3301 cggcccggac ccggaggccc ggacgatgcg cgtagtgtgc cacggaatcg cggagaggtg
     3361 caaaggaacc acggcgcttg cgccctcccc cctcggccgg tttgctaggc cgcaccggac
     3421 ccgaaggggg ggacatgagc gacgacatcg gacgcaagat gctcgcggag ttcgtgggga
     3481 cgttcgcgct gatcttcgtg ggggccggat cgatcgtggc ggcctccgtg atcgggggag
     3541 gcgacctcgc ccccaacctc gtcacgatcg cgctggccca cggcctcgtg atcgcggtga
     3601 tggtctccgc gattggccac gtgtcgggag ggcatctcaa tcctgcggtt accatcggag
     3661 catggacggc gggcaagatc aggacggggg acgccgcggc ctacctcgtg acccagctgg
     3721 cggcgggcgt ggccggcgcc ctcctgctgc gggccgtcgt cccggaggac gcatggcgct
     3781 ccgtctccct gggggccacc ctcctgggag acgtcaccgt ggcccagggg atcgcgatcg
     3841 aggcggttct gacgttcctg ctggtgtggg tgatcttcgc ggtcgcggtg gaccccgacg
     3901 ggaccttccg cgcggtggcc gggctgccga tcgggctcac ggtggcgctc gacatcatga
     3961 tgggcgggcc cttcacggga gcgtccatga accccgcgcg ctcgttcggc ccggcggtgg
     4021 ccggcgggtt ctggaccgac cactgggtgt actggatcgg gccggtggcc ggcggcgtgc
     4081 tggccgcggt gctgtacgac ctgctcgtgc tgcgtcgccg gcgtccggag cccacgctgg
     4141 aggaggctcc ctgacgaggt gagcactcct cccgaaatgc tcgaggcgct ccggctcctc
     4201 gtcgaggccg agtccttctc gtcggatccg ggcgggctgg agcggtgcgg cctcgtcctc
     4261 cgggacctat gccgggggat cttggggagc cccccggagg cggaggggca cgtgctgcgg
     4321 tgggagcgcc cgggggatcg gcccatcctc atcctctgcc actacgacac ggtgtggccc
     4381 acggacaccc tggcgcactt cccgttcgag gtccgggacg gcgtggcccg agggcccggg
     4441 gccttcgaca tgaaggcggg gatcgtccag gggctcttcg ccatccggga cagcagcggg
     4501 ccggtgacga tgctgctcac ccacgacgag gagcgtggga gcgccgagtc tcaggaggcg
     4561 atcgagcggg aggcgctgac ctcgcgggcc gtgctcgtgc tggagccctc ggccgacggg
     4621 ggcgcggtga agacggcgcg caagggcgtg gcccactggc ggatcgaggt gaagggccgc
     4681 gccgcccacg ccggcctcga gcccgagaag ggcgtgaacg cggccatcga gatcgcccgt
     4741 cagctggagc ggatctcctc gttcggggat cccgcggccg ggaccacggt caccgtgacg
     4801 atggtccggg cggggtccgc ggagaacgtc gtccccgagg aggcatgggc cagcgtggac
     4861 gcccgcgtgt ggacgcgaga ggagggcgag cgcctggaga gggccttcgg ggcgctcgag
     4921 cccctgaccg agggcgccca cctctccgtc gagggcggga tgaaccgggg ccccctcggg
     4981 cgggaggcga gcggggacct gtaccagcgg ctccggcgcc tcgggtacga cctcccggcg
     5041 gccgaggtgg gcggcgggag cgacgggaac ttcacggcgg ccctcggcgt ccccaccctc
     5101 gacgggctcg gcgccgaggg cggcggggcc cacgcccgga acgagcacgt cctcgtggac
     5161 gcgatgccct ggcgggccga gatggtggcc cgcctgatcg acgatctggc cgccgatggc
     5221 tgaggtcgag atccgagacc tcgccgagat cgacgacctc cgctccaccc tggagctgtt
     5281 cgagcggatc tggggcgccc gcgagcgcat gttcgcggtg gaggtgctcc gggccgtggc
     5341 cacccacggc ggcgcgatcc tcggggcgtt cctggacggc cggatggttg gggcccaggt
     5401 cgcgttcctg ggccgggacg agaacgagct gctccttcat tcgcacgtca cgggggtgga
     5461 gccgaccctc cagcacgagg ggatcggctc ggctctgaag tgggcgcagc gcgattgggc
     5521 gctggagcgc gggatcgagc tcatcacctg gaccttcgac ccgatgatct cgaggaacgc
     5581 gtacctcaac cttcggaagc tgggcgcctc cgcccgccgg ttcctgcgag acttctacgg
     5641 gcgtatggac gacgacatca acgtgggcga gcgctcggac cggctcgaga tcacgtggcg
     5701 cctgaaggac ccgagggtgg agggtgcggc cgcgggtggc cccgtggccc gcgacgccga
     5761 gggggccgcc gtcctgctcg acgccccgga gggccgcccg cggccgcacc ccgaggccct
     5821 cggcgcgccc ctcgtgctgg cccgcgtccc caccgactac ctggacctcc gcgcccggga
     5881 cccggaggtc gcccgggcct ggcgcgacgc cgcggccgac gtcctggaga gggcactgtc
     5941 cggcggctac gccgccgtgg acttcctccg cgagggcgcc tacgtgctgg agcggtcgtg
     6001 agcctcgagg cgatcgagct gatcctcatt cgactcccgc tgctgaggcc gttcacgacc
     6061 tcgttcggca cgctcaccga caaagaggcg atcctgatcc gggccgtgga cgaggacggc
     6121 ctcgaggggt ggggcgagtg cgcggtggcc gaggaccccc tctactccga ggagtggaac
     6181 cccggcgcct ggatgatcct ccgcaactac ctggcccccg cggcgctcga aggagcctcc
     6241 tcggggatca agggtcatcc gatggcccgg accgcgatgg aggtcgccct cctcgacctc
     6301 cgcctccgcc agaagcaggt ctcgctcgcc tcccacctcg gggccgtccg ggaccgcatc
     6361 gtctgcggcg tgtccctcgg catcgaggag tcgatcgacg agctcctgaa gcaggtcgac
     6421 cgcttcgtgg cggacggcta ccggcggatc aagctgaaga tcgcgcctgg gtgggacctg
     6481 gagccggtgg gcgcggtgcg cgaggcgttc cccgacaccg ccctctcggt ggacgcgaac
     6541 gccgcctacg ggctcgagga cgccgatcac ctggcccgcc tcgacgcgtt cgacctcgac
     6601 tacctcgagc agcccctccc caaggaccac ctcctggccc acgccgacct ccagcagcgc
     6661 atcgagacgc cgatctgcct cgacgagacc ctgacctcgc cccacgtgac cgacgaggcg
     6721 atccggctcg aggcgtgccg cgtggtc
//
LOCUS       DASUTZ010000158         3619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_435996, whole genome shotgun sequence.
ACCESSION   DASUTZ010000158 DASUTZ010000000
VERSION     DASUTZ010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435996"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="VF097_06875"
     CDS             <1..1050
                     /locus_tag="VF097_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX6262553.1"
                     /translation="TSLPEVPGGSANWDYRFSWPRDASLGISAFLGVGLGDEARAFLS
                     WLTIASRLTRPRLHVLYDLEGRPSSRERERRDVPGYRGSLPVRLGNAAADQHQLDVYG
                     WVVGAVWELHARGERLPPDVWRAVAAWADFVSGSWREPDSGIWEERGEPHHYVHSKLM
                     AWIALDRALRLSEGYRVGRSRRDRWSKEREALGAEIRSRGVDADRGVYVRHDETSELD
                     AALLTLPDVGIEGETSPRVVRTIEAVAAELGAGDGLLYRYPRSREDGEGAFLACSFWL
                     ARALSRIGQVDRAGELFESLCSRASELGLFGEELDPGTGRHLGNFPQALTHSALVSAA
                     LAIQEATRGRAGSRG"
     gene            complement(1019..1495)
                     /locus_tag="VF097_06880"
     CDS             complement(1019..1495)
                     /locus_tag="VF097_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010594167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262554.1"
                     /translation="MDWGGWSIFGFVGTVVLTTVMVFAQLIGWTRMDLPMMLGTIFAT
                     NVDRARLLGVFLHLAAGQLFALFYALAFWKLGRSGAILGGLFGLLHGLAALTVIVPFL
                     PAVHPRMASERSGPELDTALEPPGLLGLNFGVTTPLVALVAHVLFGITLGILLDPS"
     gene            complement(1531..1603)
                     /locus_tag="VF097_06885"
     tRNA            complement(1531..1603)
                     /locus_tag="VF097_06885"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1568..1570),aa:Arg,seq:tct)
     gene            complement(1641..2087)
                     /locus_tag="VF097_06890"
     CDS             complement(1641..2087)
                     /locus_tag="VF097_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262555.1"
                     /translation="MLDSTGGRGPPTTEEDAMIARRRTLWAVLAALVLTGTLFSIPAN
                     AGDDPRVERRGGCSASSRWKMKAKKDDGRIEVEFEVDQNVNGQRWRVVMKRNGNRFFR
                     GIRTTQPPSGSFSVERRTPDGPGIDRFRARARNLRTGEVCRGRVAI"
     gene            2178..2918
                     /locus_tag="VF097_06895"
     CDS             2178..2918
                     /locus_tag="VF097_06895"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX6262556.1"
                     /translation="MAKIAVLSDLHSNLEALDAVVAAARDRDATGFIVCGDLVGYGAD
                     PGAVLERVFDLPLRSVVAGNHDLAAVDRFSLDWFNDVAAEALRWTSEQLTPDARDTLA
                     GLEPREPTTEGLVVHGSVVEPASEYLMPGEDAAAARSFGREPFDRCFFGHTHVPTVFE
                     QGEDGGVRGAPIRSEGRLTLTAGHRYLLNPGSVGQPRDGDPRAAFMILHDGAAEWVRV
                     PYDAAAAGEKIREAGLPEVLADRLLVGR"
     gene            complement(2920..3300)
                     /locus_tag="VF097_06900"
     CDS             complement(2920..3300)
                     /locus_tag="VF097_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262557.1"
                     /translation="MARQRGALPLAVLIAGTLIAGVTTAVIRVTEDEAPQAVPTESPS
                     PTATSPSPEATDEPSPEPTEEPTEEPTEEPTEEPTEEPTVDDGDGGGGLAETGTESLP
                     FVLGGVLMIGLAGALWRLSRPPVD"
     gene            complement(3378..3448)
                     /locus_tag="VF097_06905"
     tRNA            complement(3378..3448)
                     /locus_tag="VF097_06905"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3414..3416),aa:Gly,seq:tcc)
     gene            3500..3573
                     /locus_tag="VF097_06910"
     tRNA            3500..3573
                     /locus_tag="VF097_06910"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3534..3536,aa:Pro,seq:tgg)
BASE COUNT          508 a         1255 c         1328 g          528 t
ORIGIN      
        1 acgtcgctgc ccgaggtgcc gggcgggagc gcgaattggg actaccgctt ctcctggcct
       61 cgggacgcga gcctcgggat ctccgcgttc ctcggggtcg ggctcgggga cgaggctcga
      121 gcgttcctct cgtggctgac gatcgcctcc cggctcaccc gccccaggct gcacgtcctc
      181 tacgacctgg agggacgccc gagctcgagg gagcgggagc gtcgagacgt ccccgggtat
      241 cgtgggagtc tccccgtccg gctcgggaac gccgccgccg accagcatca gctcgacgtg
      301 tacgggtggg tcgtgggcgc cgtatgggag ctgcacgcac gcggggagcg tctgccgccc
      361 gacgtgtggc gcgcggtggc ggcatgggcc gatttcgtgt ccgggtcctg gcgagagccg
      421 gactccggca tctgggagga gcgcggcgag ccgcaccact acgtccactc gaagctcatg
      481 gcctggatag ccctggaccg ggccctgcgg ctgtcggagg gctacagggt gggccggtca
      541 aggcgggacc gttggtcgaa ggagcgggag gcgctgggag ccgagatccg ctcgcgaggg
      601 gtggacgccg atcggggtgt ctacgtccgc cacgacgaga cgtcggagct cgatgcggcc
      661 ctcctgacgc tcccggacgt cgggatcgag ggggagacct ctccgcgggt cgtccggacg
      721 atcgaggccg tcgcggcaga gctcggcgcc ggcgacggcc tgctctatcg ctacccccga
      781 tccagggagg atggcgaggg cgcctttctc gcctgctcct tctggctggc gcgagccctc
      841 agccggatcg ggcaggtgga tcgagcgggc gagctcttcg agtcgctgtg cagccgcgcg
      901 agcgagctgg ggctcttcgg ggaggagctc gatccgggga cgggacggca cctcggcaac
      961 tttccgcagg cgctcaccca ctcggctctg gtgagcgccg cgctcgcgat ccaggaggct
     1021 acgaggggtc gagcaggatc ccgagggtga tcccgaacag cacgtgggcc acgagcgcga
     1081 cgaggggcgt cgtgaccccg aagttcaggc ccaggagccc cggcggctcg agcgccgtgt
     1141 cgagctcggg gcccgagcgc tcggaagcca tgcgtggatg gaccgccggc aggaagggca
     1201 cgatcaccgt cagggcggcc aatccgtgga gcagcccgaa gaggcctccc aggattgcgc
     1261 ccgaacggcc tagcttccag aacgccagcg cgtagaagag cgcgaacaac tggcccgccg
     1321 cgagatggag gaagacaccg agcagccgcg cgcggtcgac gttcgtggcg aagatggtcc
     1381 cgagcatcat cgggaggtcc atccgcgtcc atccgatcag ctgggcgaac accatgaccg
     1441 tggtgagcac cacggtcccc acgaagccga agatcgacca cccgccccag tccatcgacg
     1501 ccatcgtagg tgaggagggg tctcgtgaag cgcgccggga gggattcgaa cccccgtccc
     1561 gctgcttaga aggcagccgc tctgtcccct gagctaccgg cgctgccacc agtgtagatc
     1621 gtccggacct cccgtccgcg tcagatcgcg acgcgccctc ggcacacctc acccgtgcgg
     1681 aggttcctgg cccgggcccg gaagcggtcg atcccaggcc catcgggggt gcgacgctcc
     1741 acgctgaacg agccgctggg cggctgcgtc gtgcggatgc cccggaagaa tcggttcccg
     1801 ttgcgcttca tgaccacgcg ccaccgctgg ccgttcacgt tctggtccac ctcgaactcg
     1861 acctcgatcc ggccgtcgtc cttcttcgcc ttcatcttcc accggctgct cgccgagcag
     1921 ccgcccctgc gctccacgcg gggatcgtcc ccggcgttgg ccgggatcga gaacagcgtc
     1981 cccgtcagga cgagcgcggc cagcacggcc cagagcgtcc gtctcctcgc gatcatcgcg
     2041 tcctcctcgg tcgtgggcgg ccctcggccg ccggtcgagt cgagcatgcg gcccccgcct
     2101 gaggcggttc tgaagcgcgc ctgagggttc cttcagccct tcgtgcgcgg agcccggaag
     2161 gcgggcagac ttgaggggtg gcgaagatcg ccgtcctctc cgacctgcac tcgaacctgg
     2221 aggcgctgga cgcggtcgtg gcggccgcgc gagaccggga cgccaccggc ttcatcgtgt
     2281 gcggcgacct cgtcggatac ggggccgacc ccggagcggt gctggagcgc gtcttcgacc
     2341 tccctctccg gtcggtcgta gccgggaacc acgacctggc cgccgtggat cgcttctcgc
     2401 tcgactggtt caacgacgtc gcggccgagg ccctgcgctg gacgagcgag cagctcaccc
     2461 ccgacgcccg cgacacgctg gccggcctgg agccgcgcga gcccacgacc gagggcctcg
     2521 tcgtgcacgg ctccgtcgtc gagcccgcga gcgagtacct gatgcccggc gaggacgcgg
     2581 ccgccgcccg gagcttcggg cgcgagccct tcgaccggtg cttcttcggc catacgcacg
     2641 tccccacggt gttcgagcag ggggaggacg ggggtgttcg gggtgccccg atccggagcg
     2701 aggggcggct cacgctgacg gccggtcacc gctacctcct caaccccggg agcgtgggtc
     2761 agcctcgaga cggcgatccc cgggcggcgt tcatgatcct ccacgacggg gcggcggagt
     2821 gggtccgcgt gccctatgac gcggcggcgg cgggggagaa gatcagggaa gcgggactgc
     2881 ccgaggtcct ggccgaccgt ttgctcgtgg ggcgctgagt cagtcgacgg ggggacgcga
     2941 gaggcgccag agggcgccgg ccaggccgat catcagcacg cctcccagga cgaaggggag
     3001 cgactccgtc ccggtctcgg cgagcccgcc gccgccatcg ccgtcgtcga ccgtgggctc
     3061 ctcggtcggc tcctccgtcg gctcctccgt cggttcctcc gtcggttcct ccgtgggctc
     3121 gggcgagggc tcgtcggtgg cctcggggga cggagaggtg gccgtggggg aggggctctc
     3181 ggtgggaacg gcctgcggcg cctcgtcctc ggtgacgcgg atcaccgccg tcgtcacgcc
     3241 cgcgatgagg gtccccgcga tgagcaccgc caacggcagc gcgcctcgct gtcgtgccac
     3301 ggatctcctt ccccccagtg ccccgcggat tctaacccga aggagagcgg gcctcggccc
     3361 ggttgccgag cggatcgagc gggtgagggg aatcgaaccc ccgtaaccag cttggaaggc
     3421 tggggctcta ccattgagct acacccgcga cgcatctagt gtaccggcct cgggtctccg
     3481 ggccgtagaa tcgagcgagc ggggcgtggc gcagcttggc agcgcgcgtg ctttgggagc
     3541 acgaggtcgg cggttcgaat ccgcccgccc cgaccggcga tgaggagccg ggctccggat
     3601 cgtcactatg gacgaaggc
//
LOCUS       DASUTZ010000159        15794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1213158, whole genome shotgun sequence.
ACCESSION   DASUTZ010000159 DASUTZ010000000
VERSION     DASUTZ010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 15794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15794
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213158"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VF097_06915"
     CDS             complement(<1..434)
                     /locus_tag="VF097_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020106983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX6262558.1"
                     /translation="MATENQITIVGNLTDDPELRYTPNGAAVANFRVAVSRRYKDPQT
                     NEWKDADTSFFTVNVWRSMAENAAETLTRGSRVVVVGRLKQRSWETAEGDKRTVIEIE
                     ADEVAPSLKWATAKIEKQSRGGGGASDWNENVSVGAGGPAGET"
     gene            complement(508..795)
                     /gene="rpsF"
                     /locus_tag="VF097_06920"
     CDS             complement(508..795)
                     /gene="rpsF"
                     /locus_tag="VF097_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX6262559.1"
                     /translation="MRTYEVMVIVDESAEDKLEAVVERVTQIVTNGGGEVTKVDRWGK
                     RKFAYEIDHKTDGFYLLLEFTADTELLGELERVLLLADEVVRHKVVRREAA"
     gene            1053..1628
                     /locus_tag="VF097_06925"
     CDS             1053..1628
                     /locus_tag="VF097_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018835642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX6262560.1"
                     /translation="MSSRRSIRHFAPDPIDPELIRLAIAAAASAPSGANVQPWRFVVV
                     TDPALKRRIRDGAEKEERDFYEGRAPAEWLEALAPLGTDWRKPHLEDAPCLIVVFEVH
                     KGEGEPKPYYAKESVGIAVGFLLASLHHAGLATLTHTPSPMGFLGEILGRPPNERPFA
                     VIPVGRAAQDAEVPDITKKSLDEVVVWLGPE"
     gene            complement(1629..2372)
                     /gene="heR"
                     /locus_tag="VF097_06930"
     CDS             complement(1629..2372)
                     /gene="heR"
                     /locus_tag="VF097_06930"
                     /inference="COORDINATES: protein motif:HMM:NF037099.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heliorhodopsin HeR"
                     /protein_id="HEX6262561.1"
                     /translation="MRALRWWNASLAVLHLVQGALMWAISNDFSLPVTVAFLTGGPDG
                     IVSEPETLFTVRIGPLVASFLFLSGLAHLSLATFARAWYERMLHREIQPARWIEYSAS
                     SSVMIVVIAMLTGVYDVAALLALFAVNACMILFGWVTETRNEGRERVDWMPFWFGVFA
                     GAIPWLAIGIYLLGAGGDGAGPPGFVYGIYVSLFVFFNVFAINMVLQYRRVGRWRDYL
                     YGERAYMLLSLFAKSALAWQVFVGTLRPS"
     gene            complement(2369..3277)
                     /locus_tag="VF097_06935"
     CDS             complement(2369..3277)
                     /locus_tag="VF097_06935"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX6262562.1"
                     /translation="MASRILVTGASGTIGSRLVERLRGADVPVRLAGRSPEALRRRWP
                     PLDAVRLDALDPATIPAAMRDVDVLYYLIHSMGEGEAGFAERDRRAAANVAGAAAEAG
                     VRRIVYLGGLGDPVDSLSEHLASRQEAGRVLAAHGPPVVELRAGIILGADSASFRMLW
                     DLVDRLPVMVTPRWVDTRAQPIAIDDVIGYLDAARTVETAQRHLIVEIGGADVLSYKE
                     MMQRLARIRGKRRRIVTVPVLTPRLSSLWCAFVTSVPLSVARPLIDGVRNEVVVRDDT
                     ARLLFPDIRPVGFEAAARAAMEEMHR"
     gene            complement(3305..4039)
                     /gene="panB"
                     /locus_tag="VF097_06940"
     CDS             complement(3305..4039)
                     /gene="panB"
                     /locus_tag="VF097_06940"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009950170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX6262563.1"
                     /translation="MLTAYDYPTARILDEAGIPVLLVGDSLANVVLGHPTTLPVTMEE
                     MLHHTRAVARGATSALVVGDLPFLSYQASHEEAIRNAGRFLKEADAHAVKLEGAHPEL
                     IERMVSAGIPVMGHLGLTPQSVHAMGGYRVQARTEEAAERLLADAAQIEKAGVFSIVL
                     EGVPSSVAKRATESLAVPTIGIGAGPHCDGQVLVLHDLLGLTFGKPAKFVKRYADLGG
                     AISQAVSAFKGEVEGGAYPGPEHSYD"
     gene            complement(4159..4755)
                     /locus_tag="VF097_06945"
     CDS             complement(4159..4755)
                     /locus_tag="VF097_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262564.1"
                     /translation="MRRAWVLAISMALLTAACAEQSGDAESTPTPTETPSPTVSPMPT
                     ATSDDLEDASCDDRQGGDPNNFPDFVEVSIESEGDTEKVEFRFEPEGDPSDAPQYFVG
                     FTDQLITDGEGAPVDVAGEAFVSISFMAQGVDLSGEEFEPIYTGPTEFTPDLSTVLEV
                     EQTGDFEGLVSWGIGLSSRACFVVDARPDRIILEFPAS"
     gene            complement(4776..6629)
                     /locus_tag="VF097_06950"
     CDS             complement(4776..6629)
                     /locus_tag="VF097_06950"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEX6262565.1"
                     /translation="MAAPAAARRSLAVGALLVLAFAIGQPPADAKSYRFPEVRIEASV
                     LHDGTIELQERRTFEFDGSFSTGFFTVEPAGTPYARAEGFRVEEDGVEIPTTVTTGAG
                     GSLRAEWSFRAEDERRTFDISYRIRCAVLVYDDAAHLLWQFVGTGWEVPTDHVLVRVR
                     LPGRATAAPERPVGCPEAVPEAPATAPLERGDVRAWGHGPLGGDVRLPDPQTVELEIR
                     DLEPFTFVEGSILMPSEVVPAAPSYPGGPGRGRILAEERRLAREANELRERRSRDRSI
                     SFGMLAGVPLAFLALVLISKARDRVPGVPQHLEEPPEDLHPVELATLWDAAAGRYVSR
                     NTYRTQLLHLARAGAIELEAVGRVTDPDDIVVRLKERPSSGMDEKFTSFLFPDSERTV
                     KLGDLEARGKRRKRIAAWKDAVTGKTSAMVDRVRKEGPRWESILMLVVVGIGIWWWTK
                     ADLAGWTGLAVAGLAVLGWLVALRFMTPRLPPDLRERVARWGAFRRFLREFSSLPEAP
                     ALAVVIWERYLVYASALGVADEVERQVADLVPPEELPSPWGGAPRGLAGLDLVHSMRV
                     ATPLLAPSPPSPRGGSSFSSGVGSFSSSGGFGGGFSGGGGGGGGGTGGGAR"
     gene            complement(6601..8490)
                     /locus_tag="VF097_06955"
     CDS             complement(6601..8490)
                     /locus_tag="VF097_06955"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEX6262566.1"
                     /translation="MSTRGRLVLVLVALLPIMLTTGVVSGWIGPLGKSYHFPRVLIGA
                     TIEPDGTLALRERRTYDFRGSFSSAFFTIDPTHAPTANIRDFTVREGGRVIEHQEGVS
                     DRGGFQATWFYSAQDERRTFTISYRVRCAVDVYDDAAHLNWQFIGRDWTEPTDLARIT
                     VHVPDPARRPAPRQGVCPLPESAAQPAATRTRPLRDGEVRAWGHGPLAGTVRFEDPGT
                     VELEVRDLRPATFVEGSILFPLDSVPTAAAIPGGPGTDRIVAQERLDAEDANALRGRH
                     RFMSGLTTVLLYLVPALMLLLVIVAFRRDRVPDVPPTLQEPPEDVHPVKLTLTWNTFH
                     KRLGARDAYRAQFLHLVHEGAIRLDAEGMVTDPKEIHIRRGRMPEDPMDRAFVYFLFA
                     EGEEPRLSRLRSRGKRKELLGDWWEKVGAETKRTVTAISKARGRAESRAMAALAIAAS
                     VFGFWAWTGFSESLEPLGIVGPPAAWLIPISAISWVISSSFLRPYPSNAMRRRIARWQ
                     AFRRFLRTFSTLEDAPTLAVIVWERYLVYAVALGVADRVEKQVRGLIPEERMAELAPE
                     GVPSTWHHWSGRVTHQRSHVASGAAATVGWASGWGGSSSGGGGGGGFSGGGGGGGGGT
                     GGGAS"
     gene            8541..9707
                     /locus_tag="VF097_06960"
     CDS             8541..9707
                     /locus_tag="VF097_06960"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEX6262567.1"
                     /translation="MGRDRTLSIVLGVTLLTLTLGFLQKAPCLGPWDGRQYTLLCYSD
                     IVPLFHTERLDEGAVPYLEADNEYPVLTGMAMALAGVPADSPSSFFVWNALMLAALGL
                     MAAAALARIAGRRALLFAAAPTLLIYAFVNWDLVAVAAATLGTWAFLRRRDGLAGLAL
                     GVGAAAKLYPALLVIPFALHRLGERRADRAGALTGAAAGAWVAINLPFALLAPERWSI
                     FFRFSAERPADWDSLWHVTQRHLSWPGSTEAVNLLSGLAFGALAAGLWLAASRTRPGF
                     PAWTFGFPLLVAFLITGKVYSPQFSLWLLPWFALALPHLPTFLAFSAAEVAVFVTRFR
                     WFAEMAGQESGVPFWAFEAAILVRAAVLVWALVLWVRRPTPQPEPERRLQEAAT"
     gene            9704..10918
                     /locus_tag="VF097_06965"
     CDS             9704..10918
                     /locus_tag="VF097_06965"
                     /inference="COORDINATES: protein motif:HMM:NF016104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HEX6262568.1"
                     /translation="MRTEDQAAATGAEPDPAAEQDRVRRGLRYSLLAFLGVRMALSIV
                     ALAGTALLPSQEPVGPQGWEGSPIVGGWHNLVTSWERFDALWFLRIADQGYFDGDGSA
                     VFFPGYPLLIRGVSFLLGDRPLAAGLLVSNLAFAGALIVLYFLTSAEWGERVARRSVL
                     YLAVFPTAFFFLAPYSESPFLLFALLAFWGARRGRWAVAGLAGAAAAATRNVGVLLAL
                     PLLIEGYQQWRAGRSRVPLLGGIAAAAAIGLTVGAYLLFWKLKVGDWWAPLTQQVNWE
                     RQRVFPLESLWLGTREAFRWIGVYPGGYHLLDWLLVIPALVAAGWVAVRTRLSFAVYT
                     WSSLLVPLSFTFLPRPFLSMPRFLLVIFPLVWASAVWAERRRGVHEAVLVTFSLGLGV
                     MTVLFAAWYFIF"
     gene            10981..11619
                     /locus_tag="VF097_06970"
     CDS             10981..11619
                     /locus_tag="VF097_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6262569.1"
                     /translation="MPVTVVIADDHQLVRQGLRRYLETAEDITVVGEASGGVEAVEVV
                     GELQPDVVVLDVRMDDLDGIEATKRIRESSPDTGVVILTGHEEERVVLEAVKAGARGY
                     VLKARDAEHLVNTVRLVAGGNMVIDPDAVGAVAERLDGETGRAGGLGDLTKRELEVLR
                     LLAMGHTNRKIAERLFISPDTVKTHLEHIYQKLGAPDRTAAVAEAMRRQLID"
     gene            complement(11616..13880)
                     /locus_tag="VF097_06975"
     CDS             complement(11616..13880)
                     /locus_tag="VF097_06975"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX6262570.1"
                     /translation="MAIRTIPRKPSRKHAPRKGASSRSLKRGSKRQRQRRRGPVRRWI
                     RKWWWLWAPPLVLGLVGVGALVYLYVTIPLPPERVLAQTTFLFDREGNRLTTLDGPED
                     RSIVELDRVPQHVRQAVLAAEDSRFYDHPGISVVDIIRAGWVDLTGGRTTGASTVTQQ
                     LVRNVFPQVGTERTITRKVKEAILALKLERELEKNEILERYLNTIYLGDGTYGFQAAS
                     RHYFGKPVAGINVLQAATLAGMIARPEGFNPRVNPGNAMVRRNFVLDRMAELGSIPRT
                     RAEKLKQRPVRTRKLEEGFADSPAAWFIDYARQWLTNQRLSPLESPREQTYQGGLRIR
                     STIDMQWQQAAINAIRNRYSYDGAPDVALVAIDPHNGEIRAVASSDDYSADPEERVFH
                     PATFGCSISRGCGWKVDANGDGQKERQSATGRQTGSAFKMFTVGTAVERGVSLQTTFS
                     GASPIRLDEPPCGSEADPWEPENFGGSSYGAMNLYGATASSVNTIFAQLISHVGPDNV
                     AEFAHRMGIKSKIPEYCSITLGAVEVNVMEMTSAFATLANGGVRHEPSPVQAIRSPGG
                     EVLWRNRGKGERVMDENDAWQTVDALEGVMNGTAAGAGFGVPAFGKTGSEDDATDGWF
                     CGSTSELTACVWVGHHEGQIPIPGLTGGGWAAPVWHDFMVAAHANLEAEPFPTPELTG
                     EIVQPEVPLPAATPSPSPEPEEEEEPEAEPTKSPKPPPSPEPEPTEPPSPSPSPTQAT
                     NPGGAGRSGDPPDP"
     gene            complement(13926..15569)
                     /locus_tag="VF097_06980"
     CDS             complement(13926..15569)
                     /locus_tag="VF097_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262571.1"
                     /translation="MTQALVPAAILGPAAAALVWLALVRWIWPRRLVAGAVWIASQAA
                     AWAVFWEVFGGEQLGWMGLGPDLLRATLAAVTGAAVMLVLPRIEALAPRASSAGMAAA
                     GVGLAAVVAAAYSSSLVSLAVLAPLPTIAVGLAGAVGDRPRGAPGLLGLAAADAAVVG
                     GLLIVLERSGTTGLEAVGSLDLGVALVLLGAAVKLGALPHVGTWKASVGEGIVSPLAV
                     VLRGQGAVLAAVAAFTLARVSPSAPLAAVSAALAVLAGLAGLAARSPEGSLAAVGGAG
                     AAAALVALGLGGGVGARAALVLLPPFVLAAGVVASSGWPGLSDVPGFRDPRERRRAWR
                     WVAALALAIGAASLVGLPTAGGFPGTWLVLSLASARAVSEPWHLALALGLVSGLALAG
                     VGAIAALRSAGARPLPAVLSLLGGLALLYAGAQPLRLGLGWLLRVERELGLGEVLPSA
                     GAPALPAIGGVNLVWVVAPALLIVAAGLVLGRGGTNPAAEHESLLALSPPAPPRFARR
                     ATGLARRLRLGRTLAALLEGAAVAGAAWIVLRGIDLGFL"
     gene            complement(15566..>15794)
                     /locus_tag="VF097_06985"
     CDS             complement(15566..>15794)
                     /locus_tag="VF097_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262572.1"
                     /translation="LAALVPAFAVGFDLVEGGDAPALARWAGVALAACAAVGAIWGAL
                     DRLGPRLRLRALVPALLAASAAAVVLVSAGR"
BASE COUNT         2277 a         5778 c         5579 g         2160 t
ORIGIN      
        1 gtctccccgg cggggccgcc ggccccgacg ctgacgttct cgttccagtc ggaggcgccg
       61 ccgcctccac ggctctgctt ctcgatcttc gccgtggccc acttcaggct cggcgcgacc
      121 tcgtcggcct cgatctcgat gaccgtgcgc ttgtcgccct cggccgtctc ccaggagcgc
      181 tgcttcagcc gccccacgac gaccacccgc gagcctctcg tcagcgtctc ggcggcgttc
      241 tcggccatcg agcgccacac gttcacggtg aagaacgaag tgtccgcgtc cttccactcg
      301 ttcgtctgcg ggtccttgta ccggcggctc accgccacgc ggaagttcgc cacggccgcg
      361 ccgttcggcg tgtaccgcag ctcggggtcg tcggtcaggt tgccgacgat cgtgatctgg
      421 ttctccgtgg ccatctggca tcaccaccct cgcctcgctc gccctcccgg ccgacggccc
      481 tgccgggagg aggggaagaa ccctcgccta cgcagcctcc cgcctcacga ccttgtggcg
      541 aaccacctcg tcggccagca gcagcacccg ctcgagctcg cccagcagct ccgtgtcggc
      601 cgtgaactcg agcaagaggt agaacccgtc cgtcttgtgg tcgatctcgt aggcgaactt
      661 ccgtttgccc cagcggtcga ccttcgtgac ctcgccgccg ccgttcgtga cgatctgcgt
      721 gacgcgctcg acgaccgcct cgagcttgtc ctcagcggac tcgtcgacga tgaccatgac
      781 ctcgtacgtt cgcaatgcca cctcctctgg actcatgcgg ccccccgggg gagcaggagt
      841 ggggtcccac gcagtctagc accggccacg gcgatgtctc gtccggcact atagcgaggg
      901 ggtctgacac gaacgagggc ttcccgcccc ccgggaagca tggtaggaaa ccggacatga
      961 cgtgcgaccc tcgccaccgg accatcccgg ccccccgccg ggagctgaca cccgaggatg
     1021 gcctcgccgc cgcccgacgg ttcctcgaga cgatgtcctc caggcgctcg atccgccact
     1081 tcgcgcccga ccccatcgat ccagagctga tccggctggc gatcgccgcc gccgcctccg
     1141 ccccctcggg ggcgaacgtc cagccctggc ggttcgtcgt cgtcaccgac cccgcgctca
     1201 aacggaggat ccgggacggg gccgagaagg aggagcggga cttctacgaa ggccgagcgc
     1261 ccgcggaatg gctcgaggcc ctcgccccgc tggggaccga ctggaggaag ccgcacctcg
     1321 aggacgctcc gtgcctcatc gtggtgttcg aggtgcacaa gggggagggc gagcccaagc
     1381 cctactacgc caaggaatcg gtggggatcg cggtggggtt cctcctcgcc tcgctccacc
     1441 acgccgggct cgccacgctg acccacacgc cgagcccgat ggggttcctg ggcgagatcc
     1501 tgggccgccc gcccaacgag cggccgttcg cggtgatccc cgtggggcgg gcggcccagg
     1561 acgcggaggt ccccgacatc accaagaagt cgctcgacga ggttgtcgtc tggctcggac
     1621 ccgaatgatc agctcggccg gagcgttccc acgaacacct gccacgccag ggccgacttg
     1681 gcgaagagcg atagcagcat gtaggcccgc tccccgtaga ggtagtcccg ccaccggccc
     1741 acgcgccggt attgcaggac catgttgatg gcgaacacgt tgaagaagac gaacagcgag
     1801 acgtagatcc cgtagacgaa gccgggcggc ccggcgccgt cgcctcccgc cccgagcagg
     1861 tagatcccga tcgcgagcca ggggatcgcc ccagcgaaga caccgaacca gaacggcatc
     1921 cagtcgaccc gctcccggcc ctcgttccgc gtttcggtca cccatccgaa gaggatcatg
     1981 caggcgttca cggcgaagag cgcgagcagc gccgccacgt cgtagacccc ggtcagcatg
     2041 gcgatcacga cgatcatcac cgaggaggac gcggagtact cgatccaccg tgccggctgg
     2101 atctcccgat gcagcatccg ctcgtaccat gcgcgggcga acgtggccag cgagaggtgg
     2161 gccagacccg aaaggaagag gaagctggcc accagcggcc cgatgcgcac cgtgaacagg
     2221 gtctccggct cggagacgat cccgtcgggc ccgccggtca ggaaggccac ggtcaccggg
     2281 agggagaagt cgttcgagat cgcccacatc agggccccct ggacgaggtg taggacggcc
     2341 agggaggcgt tccaccaccg cagcgctctc atcgatgcat ctcctccatc gccgcgcggg
     2401 ctgcggcctc gaagccgacg ggtcggatgt cggggaacag cagacgtgcg gtgtcgtcgc
     2461 ggaccaccac ctcgttgcgc acgccgtcga tcagaggacg agccaccgag agcggcaccg
     2521 aggtcacgaa ggcgcaccac agcgaggaga gcctcggcgt gaggacgggc acggtgacga
     2581 tccgcctccg cttgcccctg atccgcgcca gtcgctgcat catctccttg taggagagga
     2641 cgtcggcgcc gccgatctcc acgatcagat ggcgctgggc cgtctccacc gtcctcgccg
     2701 cgtcaaggta gccgatgacg tcgtcgatgg cgatgggctg cgcgcgagtg tccacccatc
     2761 tgggggtcac catgaccggc agccggtcca ccaggtccca cagcatccgg aacgaggcgc
     2821 tgtcggcccc caggatgatc ccggcgcgga gctcgacgac cggcgggccg tgggccgcga
     2881 gcacgcgccc ggcctcctgg cggctggcga ggtgctcgga gaggctgtca acgggatcac
     2941 ccagccctcc gaggtagacg atcctccgca cgccggcctc cgccgccgcc ccggcgacgt
     3001 tcgccgccgc ccgccgatcg cgctcggcga acccggcctc tccctctccc atcgagtgga
     3061 tcaggtagta gaggacgtcg acgtcccgca tcgcggcggg gatggtcgcg ggatcgaggg
     3121 cgtcgaggcg gaccgcgtcc aggggcggcc atcgccggcg gagggcctcc gggctccgcc
     3181 cggccaggcg gacgggaacg tcggcgccgc ggagacgctc cacgagccgc gagccgatgg
     3241 tgccgctcgc ccccgtgacg aggatgcgag acgccacgat gacgacatcc tacggcgcgg
     3301 cgggtcagtc gtaggagtgc tcggggcccg ggtaggctcc cccctcgacc tcccccttga
     3361 aggcagagac ggcttgggag atggcgccac ccaggtccgc gtagcgcttc acgaacttcg
     3421 cgggcttccc gaaggtcaga cccaggagat catgcaggac gagcacctgg ccgtcgcagt
     3481 gcggtcccgc gccgatcccg atcgtgggga cggccagcga ctcggtcgcg cgcttggcca
     3541 cggacgacgg tacgccctcc aggacgatcg agaacacccc cgctttctcg atctgcgcgg
     3601 catcggccag gaggcgctcg gcggcctcct cggtgcgagc ctgcacccgg tagccgccca
     3661 tcgcgtggac ggactgcggg gtgaggccca ggtggcccat cacggggatg ccggccgaca
     3721 ccatccgctc gatcagctct gggtgggcgc cctcgagctt cacagcgtgc gcatcggcct
     3781 ccttgaggaa ccggcccgcg ttgcggatcg cctcctcgtg cgaggcctgg tacgagagga
     3841 acgggagatc gcccaccacg agcgcgctcg tcgctccgcg ggccaccgcc cgcgtgtggt
     3901 ggagcatctc ctccatcgtc accggcagcg tggtgggatg ccccagcacc acgttggcca
     3961 gcgagtcccc cacgaggagc actgggatgc ccgcctcgtc gaggatgcgc gcggtcgggt
     4021 aatcgtaggc cgtgagcatg gcgaagcgcc tgccctcggc tttgaacgac cgcaggtcgt
     4081 tgatcgtggt cacggctcgc gcctccttcc gtgagagtgc acggcccgac aagagtaccc
     4141 cggctgggcc cgggagcgtc aggaagcggg gaactcgagg atgatgcgat cggggcgggc
     4201 gtcgactacg aagcaggccc gcgaggagag cccgatgccc cacgagacca ggccctcgaa
     4261 gtcgccggtc tgctccacct cgagcacggt cgacagatcg ggggtgaact cggtgggccc
     4321 cgtgtagatc ggctcgaact cctcgcccga gaggtccaca ccttgcgcca tgaacgagat
     4381 cgacacgaac gcctcgcccg cgacatcgac cggggcaccc tcaccgtcgg tgatcagctg
     4441 atcggtgaac cccacgaaat actgcggggc gtcgctcgga tcgccctcgg gctcgaagcg
     4501 gaactccacc ttctcggtgt cgccctcgga ctcgatgctc acctcgacga agtcggggaa
     4561 gttattcggg tcccctccct gccggtcgtc gcacgaggcg tcctcgaggt cgtccgaggt
     4621 cgcggtgggc atgggagaga ccgtggggga cggcgtctcg gtcggcgtgg gagtggattc
     4681 ggcgtccccc gactgttcgg cgcaggccgc cgtcagcaac gccatcgaga tcgccaggac
     4741 ccacgcgcgt cgcacgccca tcagtgtagg cacgttcagc gggctccgcc gccggttccg
     4801 ccgccgcccc cgccccctcc tccggagaag ccgccgccga acccacccga cgagctgaag
     4861 gacccgaccc cgctggagaa cgacgagccg cctctcggag aggggggcga gggggccagg
     4921 agcggcgtgg ccacgcgcat cgaatgcacc aggtccagcc cggccaggcc tcgaggcgct
     4981 cccccccacg gcgagggcag ctcctctggc ggcaccaggt cggcgacctg tcgctccacc
     5041 tcgtcggcca ccccgagggc gctggcgtag acgaggtacc gctcccagat caccacggcc
     5101 agcgcggggg cctcggggag agaggagaac tcccggagga agcgccggaa cgccccccac
     5161 cgggccaccc gctcccggag gtcggggggg agccgcggcg tcatgaaccg gagcgccacg
     5221 agccatccca gcaccgcgag ccctgccacg gcgagacccg tccatccggc cagatccgcc
     5281 ttcgtccacc accagatccc gatgccgacg accacgagca tcaggatcga ctcccaccgg
     5341 ggcccttcct tgcggacccg atccaccatc gcgctcgtct tccccgtcac ggcgtccttc
     5401 caggcggcga tccgcttgcg gcgcttgcct cgggcctcga ggtcgccgag cttcacggtc
     5461 cgctcggagt ccgggaagag gaagctcgtg aacttctcgt ccatgcccga ggacggtcgc
     5521 tccttgaggc gcacgacgat gtcgtcggga tcggtgacgc gtcccaccgc ctcgagctcg
     5581 atggccccgg cccgggccag gtggaggagc tgggttcgat aggtgttgcg cgagacgtac
     5641 cggccggcgg cggcgtccca gagcgtggcc agctcgacgg gatggaggtc ctcgggcggc
     5701 tcctcgaggt gctggggcac ccccggaacc cggtcccgcg ccttcgagat caggacgagg
     5761 gcgaggaacg cgagcgggac cccggccagc atcccgaagg agatcgaccg gtcccgcgag
     5821 cgtcgctccc gcagctcgtt ggcctcccgg gccagacgac gctcctccgc gaggatgcgc
     5881 ccccgcccgg gcccgccggg atacgaggga gcggccggca cgacctcgga gggcatcagg
     5941 atcgacccct ccacgaacgt gaagggctcg aggtcccgga tctcgagctc gaccgtctgg
     6001 ggatcgggaa ggcggacgtc gcctccgagc ggaccgtggc cccaggcccg aacgtcgccg
     6061 cgctcgaggg gggcggtggc gggcgcctcg ggaacggcct cggggcagcc gacgggacgc
     6121 tcgggggccg cggtggcccg cccgggcagg cgcacccgga ccaggacgtg gtcggtcggg
     6181 acctcccatc ccgtcccgac gaactgccag aggaggtgcg ccgcgtcgtc gtagacgagg
     6241 acggcacacc gtatccggta ggagatgtcg aaggtgcggc gctcgtcctc ggcgcggaag
     6301 gaccactccg cacggaggga cccgccggca ccggtggtca ccgtggtggg gatctccaca
     6361 ccgtcctcct cgacccggaa gccctcggcc ctggcgtagg gggtccccgc cggctcgacg
     6421 gtgaagaagc cggtggagaa agacccgtcg aactcgaagg tgcggcgctc ctgaagctcg
     6481 atcgtgccgt cgtggaggac cgaggcttcg atccggacct ccgggaagcg ataagacttc
     6541 gcgtccgcgg gcggttggcc gatggcgaag gcgaggacca gcagggcccc gaccgcgagg
     6601 ctacgacgcg ccgccgccgg tgccgccacc gccgccgcca cctcctccgg agaatccgcc
     6661 gcccccgccg ccccccgagg acgagccgcc ccacccgctc gcccacccca ccgtggccgc
     6721 ggcccccgag gccacgtgcg acctctgatg cgtcacccgg ccgctccagt ggtgccaggt
     6781 gctggggacc ccctccggcg cgagctcggc catccgctcc tcgggtatga ggccgcgtac
     6841 ctgcttctcc acccggtccg cgaccccgag ggccacggcg tacacgaggt accgctccca
     6901 gacgatcacc gcgagcgtcg gggcgtcctc gagcgtggag aaggtcctga ggaaccggcg
     6961 gaaggcctgc catcgggcga tgcgacgacg catggcgttg gacgggtagg gccggaggaa
     7021 tgagctggag atcacccacg agatcgcgga gatggggatc agccaggcgg cgggggggcc
     7081 cacgatcccc aagggctcga ggctctccga gaaacccgtc cacgcccaga acccgaacac
     7141 ggaggccgcg atggccagcg ccgccatggc ccgagactcg gcgcggcccc tggccttcga
     7201 gatggccgtc accgtccgct ttgtctccgc tcccaccttc tcccaccagt cgccgagcag
     7261 ctccttccgc ttccccctcg accggagccg cgagagcctc ggctcctcgc cctccgcaaa
     7321 gaggaagtac acgaaggccc ggtccatcgg gtcctcgggc atccggcccc ggcggatgtg
     7381 gatctccttc gggtcggtga ccatgccttc ggcgtcgagg cggatcgcgc cctcatgcac
     7441 caggtggagg aactgcgccc ggtaggcgtc gcgagccccg agccgcttgt ggaaggtgtt
     7501 ccaggtcagg gtcagcttga cggggtggac gtcctcggga ggctcctgga gcgtcggggg
     7561 gacgtccggc acccggtcgc gccggaaggc cacgatgacg aggaggagca ttagggcggg
     7621 cacgaggtac agcagcacgg tggtgaggcc gctcatgaag cggtggcgcc cgcggagggc
     7681 gttggcgtcc tcggcgtcca gccgctcctg ggccacgatg cggtcggtgc cggggccgcc
     7741 ggggatcgcg gcggccgtgg ggacggaatc gagcgggaac aggatcgagc cctccacgaa
     7801 ggtcgccggc cgcagatcgc gaacctcgag ctcgacggtg cctgggtcct cgaaccggac
     7861 ggtgccggcc agcgggccgt gcccccaggc gcgaacctct ccgtcgcgga ggggcctggt
     7921 ccgggtggcg gccggctggg ccgccgactc cgggaggggg cacacgccct ggcgcggggc
     7981 cgggcgtcgt gcgggatccg gcacgtggac cgtgatccgg gccagatcgg tgggctcggt
     8041 ccagtccctg ccgatgaact gccagttgag gtgggccgca tcgtcgtaga cgtccaccgc
     8101 gcagcggacc cggtaggaga tcgtgaaggt ccggcgctcg tcctgggccg agtagaacca
     8161 ggtggcctgg aacccgcccc ggtcggagac gccctcctga tgctcgatca cgcggccccc
     8221 ctcgcgcacg gtgaagtccc ggatgttcgc cgtgggcgcg tgggtgggat cgatcgtgaa
     8281 gaaggccgag gagaacgagc cccggaagtc gtaggtgcgg cgctcgcgga gcgccagcgt
     8341 gccgtcgggc tcgatcgtgg cgccgatcag gacccggggg aagtggtagc tcttgccgag
     8401 gggaccgatc cagcccgaga ccacgcccgt cgtcagcatg atcgggagga gggcgacgag
     8461 gaccagcacg agcctccccc tcgtcgacat gccggaagtg tacccgggag gtcggtccgg
     8521 tcgccgatac taggcgggtg atgggccgcg accggacgct ctcgatcgtc ctcggggtta
     8581 cgctcctcac cctgaccctc gggttcctgc agaaggcccc gtgcctgggg ccgtgggacg
     8641 ggaggcagta caccctgctc tgctactcgg acatcgtgcc gctcttccac accgagcggc
     8701 tggacgaggg ggcggtcccg tacctcgagg ccgacaacga gtacccggtc ctgaccggga
     8761 tggcgatggc gctggcgggg gtccccgcgg actccccgtc gtcgttcttc gtgtggaacg
     8821 cgctgatgct ggcggcgctg gggcttatgg cagccgcggc gctggcgagg atcgccgggc
     8881 gacgggcgct cttgttcgcg gccgccccca cgctgctgat ctacgccttc gtcaactggg
     8941 acctggtggc cgtcgcggcg gcgaccctgg gcacgtgggc cttccttcgc cgccgcgacg
     9001 gcctcgcggg actggccctc ggcgtggggg cggccgccaa gctgtacccc gctctcctcg
     9061 tgatcccgtt cgccctccac cggctcggag agcggcgggc ggatcgggcc ggggcgctga
     9121 cgggggcggc cgccggcgcg tgggtcgcga tcaacctccc cttcgccctc ctggctcctg
     9181 agcgctggtc gatcttcttc cgtttcagcg cggagcggcc cgccgactgg gactcgctct
     9241 ggcacgtgac gcagcggcac ctgagctggc ccgggtccac cgaggccgtg aacctcctct
     9301 ccggcctcgc attcggggcg ctcgcagccg ggctctggct cgcggcatcc cggacgcggc
     9361 ccggcttccc cgcctggacc ttcgggttcc cgctgctggt cgccttcctg atcaccggca
     9421 aggtctattc gccccagttc agcctgtggc tgctcccatg gttcgcgcta gccctgcccc
     9481 atctcccgac cttcctggcg ttctctgcgg cagaggtcgc ggtcttcgtc acgcgcttcc
     9541 gctggttcgc cgagatggcg gggcaggaga gcggggtacc cttctgggcg ttcgaggcgg
     9601 cgatcctggt ccgggcggcc gtgctggtct gggccctcgt cctgtgggtg cgccgcccga
     9661 cgccccagcc cgagcccgag cgccgtctcc aggaggccgc gacgtgagga ccgaggacca
     9721 ggccgccgcc accggggccg agcccgaccc ggccgccgag caggatcgcg tccgccgcgg
     9781 gctccgctac agcctgctcg cgttcctggg cgtccggatg gcgctctcga tcgtggcgct
     9841 cgccggcacg gcgctcctcc cctcgcagga gccggtcggc ccccaggggt gggagggctc
     9901 cccgatcgtc ggcggctggc acaacctcgt cacttcctgg gagcggttcg acgcgctgtg
     9961 gttcctgcgg atcgccgacc agggctactt cgacggggac ggcagcgcgg tgttcttccc
    10021 cggctacccc ctgctgatcc gcggcgtctc gttcctcctg ggggaccgtc cgctggcggc
    10081 tggcctgctc gtctcgaacc tggccttcgc cggggcgctg atcgtcctct acttcctgac
    10141 gagcgccgag tggggcgagc gcgtggcccg gcggtcggtg ctctacctcg ccgtgttccc
    10201 caccgcgttc ttcttcctcg cgccgtactc ggagtcgccg ttcctcctct tcgccctgct
    10261 ggcgttctgg ggggcgcgtc gcggccgctg ggcggtcgcc ggcctcgccg gcgccgccgc
    10321 ggccgcgacg cgcaacgtcg gcgtcctgct ggcgctcccc ctcctcatcg aggggtacca
    10381 gcagtggcgg gcgggtcggt cgagggtgcc gctcctcggg gggatcgcgg cggccgcggc
    10441 gatcgggctc acggtcgggg cctacctcct cttctggaag ctgaaggtgg gcgactggtg
    10501 ggccccgctc acccagcagg tgaactggga gcgccagcgc gtcttcccgc tcgagtcgct
    10561 gtggctgggg acccgcgagg cgttccgctg gatcggcgtc tacccgggcg ggtaccacct
    10621 cctggactgg ctcttggtga tccccgccct ggtcgccgcg ggctgggtgg cggtccggac
    10681 ccgcctttcc ttcgccgtct acacctggag cagcctcctc gtgccgctct cgttcacctt
    10741 cctgccgcgc ccgttcctct cgatgccgcg gttcctgctc gtgatcttcc ccctggtatg
    10801 ggcgtcggcc gtctgggccg agcgacgtcg aggggtgcac gaggccgtgc tcgtgacgtt
    10861 ctcgctcggg ctgggagtca tgaccgtcct cttcgccgcc tggtacttca tcttctgatt
    10921 tccccgcggc ccgggaggtc gctacgatac gcgcagccga acagtcgggg ggaaggacgc
    10981 gtgcccgtca ccgtcgtgat cgctgacgat catcagctcg tcagacaggg cctgcgccgc
    11041 tacctcgaga ccgcggagga catcaccgtc gtgggtgagg cctcgggcgg cgtggaggcc
    11101 gtcgaggtcg tgggagagct gcagccggac gtggtcgtgc tcgacgtgcg catggacgac
    11161 ctcgacggga tcgaggccac caagcggatc cgcgaatcga gccccgacac cggcgtggtg
    11221 atcctcacgg ggcatgagga ggagcgggtc gtcctcgagg cggtcaaggc cggcgcgcga
    11281 ggctacgtcc tgaaggctcg cgacgcggag cacctcgtca acacggtgcg cctcgtggcc
    11341 ggcgggaaca tggtcatcga ccccgacgcg gtgggggcgg tcgccgagag gctcgacggg
    11401 gaaaccggac gcgccggcgg cttgggcgac ctgacgaagc gggagctcga ggtcctccgt
    11461 ctcctggcga tgggtcacac caaccgcaag atcgcggagc ggctgttcat ctcacccgac
    11521 acggtgaaga cccacctgga gcacatctac caaaagctcg gagccccgga caggacggcg
    11581 gcggtggccg aggcgatgcg ccggcagctc atcgactagg ggtccggagg gtcgcccgac
    11641 cggcccgcac ccccggggtt cgtcgcctgg gtgggcgagg gcgaaggcga cggaggctcg
    11701 gtcggctcgg gctccgggga gggcgggggc ttcggcgact tcgtcggctc ggcctccggc
    11761 tcctcttcct cctcgggctc cggcgacggc gaaggcgtcg ccgccgggag gggaacctcg
    11821 ggctgcacga tctcaccggt gagctccggc gtggggaagg gctccgcctc gaggttcgcg
    11881 tgcgcggcca ccatgaagtc gtgccacacc ggggcggccc agcccccacc cgtcagaccg
    11941 gggatcggga tctggccctc gtggtgcccg acccacacgc aggcggtcag ctcgctcgtc
    12001 gagccgcaga accacccgtc cgtggcgtcg tcctcggacc cggtcttccc gaaggcgggg
    12061 acgccgaagc cggcgcccgc ggcggttccg ttcatcacgc cctcgagggc gtcaaccgtc
    12121 tgccaggcgt cgttctcgtc catcacccgc tcgcccttac cgcggttcct ccaaaggacc
    12181 tcgccccccg gggagcggat cgcctggacg ggcgagggct cgtggcgaac accgccgttc
    12241 gccagcgtgg cgaaggcgga ggtcatctcc atgacgttga cctcgacggc gccgagagtg
    12301 atggagcagt actcggggat cttgctcttg atccccatgc ggtgagcgaa ctccgccacg
    12361 ttgtcggggc ccacgtggga gatgagctgg gcgaagatcg tgttcaccga ggacgccgtg
    12421 gcgccgtaga ggttcatcgc cccgtagctc gacccgccga aattctcggg ctcccaggga
    12481 tcggcctccg agccgcaggg cggctcgtcc agccggatcg ggctcgcacc cgagaacgtc
    12541 gtctggaggc tcacgccccg ctccacggcc gtcccgaccg tgaacatctt gaacgccgat
    12601 cccgtctggc ggccggtcgc cgactgccgc tccttctggc cgtccccgtt cgcgtccacc
    12661 ttccagccgc agccgcggga gatgctgcag ccgaacgtcg cggggtggaa cactcgctcc
    12721 tcggggtcgg ccgagtagtc gtcgctcgag gccacggccc ggatctcgcc gttgtgggga
    12781 tcgatcgcca ccagcgcgac gtcgggggcg ccgtcgtacg agtagcggtt cctgatcgcg
    12841 ttgatcgcgg cctgctgcca ctgcatgtcg atcgtggacc tgatccgcag cccgccctgg
    12901 taggtctgct ccctcgggct ctccagcggg gagagccgct ggttcgtcag ccactgccgc
    12961 gcgtagtcga tgaaccaggc ggcggggctg tcggcgaacc cctcctcgag cttcctggtc
    13021 ctgaccggcc gctgcttcag cttctcggcg cgggtccggg ggatggagcc cagctccgcc
    13081 atccggtcca ggacgaagtt ccttcggacc atcgcgttgc cggggttgac cctggggttg
    13141 aagccctcgg ggcgggcgat catgccggcc agcgtcgccg cctgcagcac gttgatgccc
    13201 gccaccggct tcccgaagta gtggcgcgaa gcggcctgga acccataggt gccgtccccc
    13261 aggtagatcg tgttgaggta gcgctcgagg atctcgttct tctcgagctc ccgctcaagc
    13321 ttgagggcga ggatcgcctc cttcaccttc cgcgtgatcg tccgctcggt ccccacctga
    13381 gggaacacgt tgcggacgag ctgctgcgtg acggtggagg cccccgtggt cctcccgccg
    13441 gtgaggtcca cccatcccgc gcggatgatg tccaccaccg agatcccggg atggtcgtag
    13501 aagcgggagt cctccgcggc cagcacggcc tgccgcacgt gctggggcac gcggtccagc
    13561 tcgacgatgg agcggtcctc cggtccatcc agggtggtga gccggttccc ctcacggtcg
    13621 aacaggaacg tggtctgggc cagcacgcgc tctggcggca gggggatcgt gacgtagagg
    13681 tagacgagcg ccccgacgcc gaccaggccc agcacgagcg gcggcgccca gagccaccac
    13741 cacttacgga tccagcggcg cacggggcct cgacggcgct gacgctggcg cttggacccg
    13801 cgcttcagcg atcgagagga cgcgcccttc cgcggggcgt gcttcctcga aggcttcctc
    13861 gggatcgttc ggatggccat cggagtcgag tctacccatc gagctcggat ttcgacctca
    13921 ggtcgtcaca ggaacccaag gtcgatgccg cggagcacga tccaggcggc gccggccacg
    13981 gccgccccct ccaggagggc ggcgagcgtg cgccccaagc ggagacggcg ggccagcccg
    14041 gtggcccgac gggcgaaccg gggaggcgcc ggaggagaga gcgccaggag ggactcgtgc
    14101 tcggccgcgg ggttcgtccc cccgcggccg agcacgagcc ccgcggccac gatcagaagg
    14161 gccggggcca cgacccagac caggttcacc ccgccgatcg ccggcagagc cggagcgccg
    14221 gccgaaggca gcacctcccc gagccccagc tcgcgctcga cgcggaggag ccagccgagc
    14281 ccgagccgca gcggctgggc ccctgcgtag aggagggcca gccctcccag gagcgagagc
    14341 acggccggga gggggcgggc ccccgccgat cggagcgccg cgatcgcccc gacgcctgcg
    14401 agggccagcc cggagaccag gcccagggcc agcgccaggt gccacggctc cgagaccgcc
    14461 cgggccgagg ccagcgacag gacgagccag gtcccgggga agcccccggc ggtcgggagg
    14521 cctaccagcg aggcggcccc gatcgcgaga gccagggccg cgacccaccg ccaggcgcgc
    14581 cgccgctcgc gcgggtctcg gaaacctggg acgtcgctca agcccggcca cccggaggat
    14641 gcgacgacgc ccgcggcgag cacgaatggc gggagcagga cgagggcggc gcgggcgccg
    14701 acgcccccgc ccaggccgag ggccacgagc gcagcggcgg cgcccgcgcc gcccaccgcc
    14761 gccagcgagc cttccgggga ccgggcggcg agcccggcca ggcccgcgag cacggccagc
    14821 gcagccgaga cggcggccag tggcgccgac ggcgagaccc gcgcgagcgt gaaggcggcg
    14881 acggccgcca gcaccgcccc ctggccgcgc aggacaaccg ccagcggtga cacgatcccc
    14941 tcgccgactg aagccttcca cgttcccacg tggggcagcg cccccagctt gacggccgcc
    15001 cccagaagca cgagcgcgac cccgagatcc agggagccca cggcctccag cccggtcgtg
    15061 ccggaccgtt cgagcacgat caggagcccg cccacgacgg ccgcgtccgc cgcggcgaga
    15121 cccaggaggc cgggagcccc ccggggccga tcgcccaccg cgccagccag gccgaccgcg
    15181 atcgtgggca gcggcgccag cacggccagc gacaccaggc tcgaggagta ggcggccgcg
    15241 accaccgccg ccagccccac gccggccgcg gccatcccgg cggacgaggc cctcggcgcc
    15301 agggcctcga tccgggggag cacgagcatc accgcggccc cggtcaccgc cgccagggtc
    15361 gcccgcagga ggtccggccc caggcccatc cacccgagct gctccccgcc gaacacctcc
    15421 cagaacaccg cccaggcggc ggcctgcgac gcgatccaga ccgccccggc cacgagacga
    15481 cgaggccaaa tccacctgac gagcgcgagc cagacgaggg cggcggccgc gggccccagg
    15541 atcgccgcgg ggaccagggc ctgcgtcatc ggcccgccga gacgagcacg acggcggccg
    15601 cggatgctgc cagcagcgcc ggcaccaacg cccggagccg caggcgaggt cccaggcggt
    15661 ccagcgcacc ccagatggcg cccacggccg cgcacgccgc gagcgccact cccgcccagc
    15721 gcgcgagcgc cggcgcgtcg cccccctcga ccaggtcgaa ccccacggcg aaggccggga
    15781 cgagggcggc cagg
//
LOCUS       DASUTZ010000160         2182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1731720, whole genome shotgun sequence.
ACCESSION   DASUTZ010000160 DASUTZ010000000
VERSION     DASUTZ010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1731720"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..584)
                     /locus_tag="VF097_06990"
     CDS             complement(72..584)
                     /locus_tag="VF097_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262573.1"
                     /translation="MMRTRLMAVTVVALAAAVAAGMLAHAGEGAGTRRRPPAATDAAV
                     PAVPRHLGAGEELFLVVGGVFPKRADALAAARPFGELQGYYVAPVAQFEGLAGHLGAA
                     ISDHVLVSAFRTEEGAREFVELATAAGEAALLTPLMTNRGFVYVGLGQEADPGGGGPL
                     QRPLDEGSGS"
     gene            complement(595..1191)
                     /locus_tag="VF097_06995"
     CDS             complement(595..1191)
                     /locus_tag="VF097_06995"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX6262574.1"
                     /translation="MSFSFRRAPLLALAVAFVVGGLGGPAAAWHYGQRGWRDWHANYP
                     RRPSGYAAIVNRFGQPCSNAASKISMQWRAADNGVYYTVRFHRKLGGMKTEMVADKGG
                     VSTNLDNDVHGHIRNEHLGKHTKSGIWGYNCRLIAGSSSYSTHAWGIAVDVSSAYDVN
                     GLCGSVENKYHAQIWKDHRWRWGKAWCDPMHFQYASDY"
     gene            complement(1501..>2182)
                     /locus_tag="VF097_07000"
     CDS             complement(1501..>2182)
                     /locus_tag="VF097_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEX6262575.1"
                     /translation="GLEENNPMLGLRGCRLGILYPDIYRAQVRAILEAAVNRKKRGGD
                     PKVEIMVPLVATKEELDTVEAMIREEADGVLSRKGVELEYLVGTMIELPRAAMAAGEI
                     AESAEFFSFGTNDLTQTTYGFSRDDVEGKFLARYLEDKIMLANPFESLDVSGVGELVD
                     IACQRGRKTREDLKLGICGEHGGDPDSVHFFHRVGLDYVSCSPYRVPIARLAAAQAVL
                     GEQEQASK"
BASE COUNT          330 a          816 c          711 g          325 t
ORIGIN      
        1 gggaacgggg cggtccccgg agcacgaggc tccgaggacc gccgccgccg ccaggcaggc
       61 gagcgccctc ctcacgagcc ggacccctcg tcgaggggcc gctggagcgg gccgccgccc
      121 cccggatcgg cctcctggcc cagccccacg tatacgaagc cccggttcgt catcagcggc
      181 gtgagcagcg ccgcctcgcc cgccgccgtg gccagctcga cgaactcgcg agccccctcc
      241 tccgtccgga aggccgagac cagtacgtgg tcggagatcg ccgccccgag atgccccgcc
      301 agcccctcga actgcgccac gggcgccacg tagtagccct gcagctcccc gaacggtcgg
      361 gccgccgcca gcgcgtcggc ccgcttcggg aagaccccgc ccaccaccag gaagagctcc
      421 tcccccgccc ccaggtggcg ggggaccgcc ggcacggcgg cgtccgtcgc ggccggcggc
      481 cggcgcctcg tccccgcccc ctcccccgcg tgggccagca tgccggcggc cacggccgca
      541 gcgagcgcga ccaccgtcac cgccatcaag cgagtgcgca tcatcgtccc ctcctcagta
      601 gtcgctcgcg tactggaagt gcatggggtc gcaccaagcc ttcccccacc tccagcgatg
      661 gtccttccag atctgggcgt ggtacttgtt ctcgacgctc ccgcagaggc cgttcacgtc
      721 gtaggcggat gagacgtcta cggcgatgcc ccaggcgtgg gtgctgtagg agctcgaccc
      781 ggcgatcagg cggcagttgt agccccagat gccgctcttc gtgtgcttcc ccaggtgctc
      841 gttccggatg tggccgtgga cgtcgttgtc gaggttcgtg gacacgccac ccttgtcggc
      901 gaccatctcg gtcttcatgc cgcccagctt cctgtggaag cggaccgtgt agtagacgcc
      961 gttgtcggcc gccctccact gcatcgagat cttcgaggcg gcgttgctgc agggctggcc
     1021 gaaccggttc acgatcgcgg cgtagccgct gggtcgcctg gggtagttcg cgtgccagtc
     1081 acgccagccc cgctggccgt agtgccaggc cgccgcgggc ccaccgagac cccccacgac
     1141 gaaggcgacc gcgagcgcca ggaggggcgc gcggcgaaag gaaaaggaca ctggctcctc
     1201 acctccctgt ctcaggccgg cgggagaggg aaccggcgca tcgtcacgga acggacgaaa
     1261 ggataaaccg agccgtgagg cgaggcatcc cccggccggg ggattcggag ggatcgtccg
     1321 acgtcggaaa gaccgatggg cggggtggcc cccgcccatc gtgtcggtgt cggcccaagt
     1381 gcccgcagta ccgggccgac ggctcccagt cggctgtggg aggctcgcgg atattacgcc
     1441 tccgatgaaa gaatgacaac cccccggccg gaaaaacctc gaccgggtgg gcggagcgag
     1501 ctacttggag gcttgctcct gttccccgag caccgcctga gccgccgcca gacgcgcgat
     1561 cggaacccgg tacggcgagc acgacacgta gtccagcccc accctgtgga agaagtgcac
     1621 cgagtcggga tcccctccgt gctcgccgca gatccccagc ttgaggtcct cgcgggtctt
     1681 gcgaccgcgc tggcacgcga tgtccaccag ctcgcccacc ccgctcacgt ccagggactc
     1741 gaacgggttg gcgagcatga tcttgtcctc gaggtagcgg gcgaggaact tcccctcgac
     1801 gtcgtcccga gaaaagccgt acgtcgtctg cgtgaggtcg ttggtgccga agctgaagaa
     1861 ctccgccgac tcggcgatct ctcctgcagc catcgccgcc cgcggcagct caatcatcgt
     1921 ccccacgagg tactcgagct cgacgccctt cctgctcagg accccgtcgg cctcctcgcg
     1981 gatcatggcc tccaccgtat cgagctcctc cttcgtggcg acgagcggca ccatgatctc
     2041 gaccttggga tccccgcccc gcttcttgcg gttgacggcc gcctccagga tggcgcggac
     2101 ctgcgcccgg tagatgtccg ggtagaggat ccccaggcgg catcccctca ggcccagcat
     2161 cgggttgttc tcctcgagcc cc
//
LOCUS       DASUTZ010000161         3165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1213432, whole genome shotgun sequence.
ACCESSION   DASUTZ010000161 DASUTZ010000000
VERSION     DASUTZ010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213432"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VF097_07005"
     CDS             <1..651
                     /locus_tag="VF097_07005"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX6262576.1"
                     /translation="FSTKEAAFMAGYAAASWTKTGTMGTYGGINIGPPVTDFMDGFFA
                     GAMYYNQENGTNVEVLGWDPEAEDGLFTGDFENQDNGRRVTEDLISEGADVILPVAGP
                     VGLGTAAAVEDSGQEDVMIGVDTDWCVSAEEACPIVLTSVMKNMDVAVFNAVQSVVDG
                     SFEGGLYVGTLSDEGVGLAPFHEYEGEISEDVVAALEEIRAGIIDGSISVNGVDYL"
     gene            720..2243
                     /locus_tag="VF097_07010"
     CDS             720..2243
                     /locus_tag="VF097_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6262577.1"
                     /translation="MRLELRGITKRFPGVLANDRIDLDVSGGEIHALLGENGAGKTTL
                     MNVLFGLYHADEGEILIDDEPVRFETPRDAIAAGIGMVHQHFMLIPVFTVTENVMLGV
                     EETGAVGLLDTREARRRVEEISGRYSLEVDPDARIEDLSVGAQQRVEIVKALYREAEV
                     LILDEPTAVLTPQETEELFRVMRTLRESGVGIIFITHKLKEVLAVSDRITVLRRGRVA
                     GATRPKEADESKLASMMVGRDVRLEVEKELAQPGDPVLQVEDLTALDDRGQVAVNGIS
                     FEVRKGEVLGVAGVQGNGQTELVEAITGLRPVDAGRVMIGGKDLTRASPRDVFLAGVG
                     HIPEDRISDGLVADFPVADNLILDQYYQRPFARGWIRNDEAIHRAAEQRREAYDIRTP
                     SVDQPVSNLSGGNQQKVIVARELSREIALLVASQPTRGLDVGSMAYIHEQVIAARDGG
                     TAVLLVSSELDEVLSVADRVAVIYRGRIVGLVDPKTIGREEIGLMMAGATAGQVEGS"
     gene            2240..>3165
                     /locus_tag="VF097_07015"
     CDS             2240..>3165
                     /locus_tag="VF097_07015"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6262578.1"
                     /translation="MTDEERPEATEEQGAGPGTIEAPEDRSEGAAGPAPGETSFEAPR
                     AGGALRSLVVPVLAFITALILGGLLMVFTDQEALRAWASFVRSPIYALKVSGQVVLEA
                     YQALFTGSLGSPIGIVEALASGDLEEIQVAFRPLSETIVAATPLIFAGLSVALGFRAG
                     LFNIGAEGQMNAGAITAAFVGFTFTGLPVGIHVIVAVAAGFLGGALWGAIPGILKART
                     GAHEVITTIMLNFIALRTLDFLLGTTAFQRPDRPDPISKVVAPGSELPGITGSFLRVH
                     FGIILALLAAWGVWWLLFRTTKGFEFRAVGSNP"
BASE COUNT          484 a         1030 c         1165 g          486 t
ORIGIN      
        1 ttctccacga aggaggcggc gttcatggcc ggctacgccg cggcctcgtg gaccaagacg
       61 ggcacgatgg gcacctacgg cgggatcaac atcggcccgc ccgtcaccga cttcatggac
      121 gggttcttcg ccggggcgat gtactacaac caggagaacg gcacgaacgt cgaggtgctc
      181 ggctgggacc cggaggccga ggacggcctc ttcaccggcg acttcgagaa ccaggacaat
      241 ggtcgccgcg taaccgagga cctgatctcc gagggcgccg acgtcatcct gccggtcgcc
      301 ggacccgtcg gcctcggcac cgccgcggcg gtcgaggact ccggtcagga ggacgtgatg
      361 atcggggtcg acaccgactg gtgcgtcagc gccgaggaag cctgtccgat cgtgctgacc
      421 agcgtcatga agaacatgga cgtggcggtg ttcaacgcgg ttcagtccgt ggtggatggc
      481 tcgttcgagg gtggtctcta cgtcgggacg ctctcggacg agggtgtggg cctggccccg
      541 ttccacgagt acgagggcga gatctccgag gacgtggtgg cggccctgga ggagatcagg
      601 gccggcatca tcgacggttc gatctcggtg aacggcgtcg actacctgta gcgaaccatg
      661 atggcgaggg gggcgacggc gaacccgccg ccccccttcg ccgttccgag ggggagggca
      721 tgaggctgga gcttcggggg atcaccaagc gattccccgg ggtgctggcc aacgaccgga
      781 tcgatctcga tgtctcgggc ggggagatcc acgccctcct cggcgagaac ggcgcgggca
      841 agaccaccct catgaacgtc ctcttcgggc tctaccacgc cgacgagggg gagatcctga
      901 tcgacgacga gcccgtccgc ttcgagaccc cgcgggacgc gatcgccgcg ggcatcggga
      961 tggtccacca gcacttcatg ctgatcccgg tcttcaccgt gaccgagaac gtgatgctgg
     1021 gcgtggagga gaccggggcc gtcggtctcc tcgacacgcg cgaggctcgt cgtcgcgtcg
     1081 aggagatctc cggtcgctat agcctcgagg tggatcctga tgcccgcatc gaggatctct
     1141 ccgtcggggc ccagcagcgc gtggagatcg tcaaggcgct ctaccgtgag gccgaggtgc
     1201 tgatcctcga cgagcccacg gccgtgctca caccccagga gaccgaggag ctcttccggg
     1261 tgatgaggac cctgcgggag tcgggcgtgg ggatcatctt catcacccac aagctgaagg
     1321 aggtcctggc ggtctccgac cgcatcacgg tgctccggcg gggacgggtg gcgggggcga
     1381 ccaggcccaa ggaggccgac gagtcgaagc tcgcctcgat gatggtgggg cgcgacgtgc
     1441 gcctcgaggt cgagaaggag ttggcgcagc cgggggaccc ggtcctgcag gtggaggacc
     1501 tgacggccct ggacgaccgg ggacaggtcg cggtcaacgg catctcgttc gaggtccgga
     1561 agggcgaggt gctgggcgtg gccggggttc agggcaacgg gcagaccgag ctggtcgagg
     1621 ccatcaccgg gctcaggccg gtggacgcgg gacgcgtgat gataggtggg aaggacctga
     1681 cgcgggccag cccccgggac gtcttcctgg ccggtgtggg gcacatcccc gaggaccgga
     1741 tcagcgacgg gctcgtcgcg gacttccccg tggccgacaa cctcatcctc gaccagtact
     1801 accagcggcc tttcgcccgg ggatggatcc ggaacgacga ggcgatccac cgggcggcgg
     1861 agcagcggcg tgaggcatac gacatccgca cgccgtccgt ggaccagccc gtctcgaacc
     1921 tgtcgggtgg caaccagcag aaggtgatcg tggcccgcga gctctcccgc gagatcgccc
     1981 tcctcgttgc ctctcagccc acgcgggggc tcgacgtggg atccatggcg tacatccacg
     2041 agcaggtgat cgccgcccgc gacgggggga cggccgtgct gctcgtctcc agcgagctgg
     2101 acgaggtgct ctccgtcgcc gaccgtgtgg ccgtgatcta cagggggcgc atcgtggggc
     2161 tcgtggaccc gaagacgatc ggccgggagg agatcgggct gatgatggcc ggcgcgaccg
     2221 ccgggcaggt ggaggggtca tgacggacga ggaacggccc gaggcgaccg aggagcaggg
     2281 ggcgggcccc ggaacgatcg aagcgcccga ggaccgctcg gagggcgccg ccggccccgc
     2341 tccgggcgag acgagctttg aggcgccgcg ggccggcggc gccctccgct cgctggtcgt
     2401 ccccgtgctg gccttcatca ccgcgctcat cctcggggga ctgctgatgg tcttcacgga
     2461 ccaggaggct ctccgggcgt gggccagctt cgtccgcagc ccgatctacg cgctgaaagt
     2521 ctcggggcag gtcgtgctcg aggcctacca ggccctgttc acgggctcgc tgggcagccc
     2581 gatcgggatc gtggaggcgc tcgcctccgg ggacctcgaa gagatccagg tcgcgttccg
     2641 gccgctgtcg gagacgatcg tcgccgcgac cccgctgatc ttcgcgggcc tgtcggtggc
     2701 cttggggttc cgggcggggc tgttcaacat cggcgccgag ggccagatga acgcgggggc
     2761 gatcaccgcc gcgttcgtgg gcttcacgtt cacgggcctc cccgtcggga tccacgtcat
     2821 cgtggcggtg gccgccgggt tcctgggcgg ggcgctctgg ggggcgatac ccgggatcct
     2881 caaggcgcgc acgggcgccc acgaggtcat cacgaccatc atgctgaact tcatcgccct
     2941 gcggacgctc gacttcctgt tggggacgac cgccttccag cgccccgatc gccccgatcc
     3001 gatcagcaag gtggtcgcgc cgggatcgga gctgccgggc atcacgggct ccttcctccg
     3061 cgtccacttc ggcatcatcc tggccctgct ggcggcctgg ggcgtctggt ggctgctctt
     3121 ccggaccacc aagggcttcg agttccgagc cgtcggctcg aaccc
//
LOCUS       DASUTZ010000162        15374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_307744, whole genome shotgun sequence.
ACCESSION   DASUTZ010000162 DASUTZ010000000
VERSION     DASUTZ010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 15374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15374
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307744"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VF097_07020"
     CDS             <1..534
                     /locus_tag="VF097_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6262579.1"
                     /translation="EGNFDWIEPDDEFHAFINDLQRPVGTYLYGRRIYEVMSVFESDE
                     VFESVYAEQGVEVSPAVRDFASIWRAAEKVVYSRTLEEVTTARTRLERAFDPEAVREL
                     KASSAADLSIAGPELAAHALRAGLVDELQLTLSPVAVGGGKRALPDGLRIDLELTDEL
                     RFASGIVYLGYRVLAGD"
     gene            794..1204
                     /locus_tag="VF097_07025"
     CDS             794..1204
                     /locus_tag="VF097_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004546866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX6262580.1"
                     /translation="MRVMMMVKGDQEPGEIPSEKLLAAMGSYNEELAKAGVLLDLAGL
                     HPSAEGVRVRLSGGKTTVVGGPFADRREIVAGYWLLQVRSMDEAIEWAKRLPVEAAEH
                     DYGQESEIEIRQVFELEEFAEGPAIDRAGGLEEE"
     gene            1299..1736
                     /locus_tag="VF097_07030"
     CDS             1299..1736
                     /locus_tag="VF097_07030"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX6262581.1"
                     /translation="MSQPSNQTIVQRYMEAFPMDFDVLGELRHPDYVEDWPQSGERIR
                     GHERYVRIHEQYPGGLPESEVRRIVGSEDRWVTTPSFTLLRINGTGDTFTVEGTLTYP
                     TGNATHLVAILRLWDGKVAEVTHYFADPFEAPDWRAEWVEPIP"
     gene            1813..3441
                     /locus_tag="VF097_07035"
     CDS             1813..3441
                     /locus_tag="VF097_07035"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX6262582.1"
                     /translation="MEPRELLDRFWDEFLGLEPVFATLVGDERFDDRLPDPSPEGLAR
                     RESVHRSTLEALGDVDRTGLGMEERTALDMAESLACRDLAIVELRLDRLWAVSHMQGS
                     YLCGPGLLPGHLGALQRADTPERVDRYLARLSAVPAYLEAIRGVMREAIDAGQVAPAV
                     VVDRSIGLVERQLEAGAEASPAMQPATEGADRDRVAAVLADVVLPAYADYLEALRAYR
                     PSARETLGLHALPGGEEMYAAEILGWTGLPLDTGELHELGRSQLDSIRAEGAAIASGL
                     GFPDPAEAIAARADHEIARDAVLLVAREQVERGWEAAKGFFGRLPARNCEVRAVPPDL
                     EEDALDYYQGPTDDWSRPGVYWVNTAPRPRHSLAPTTYHEATPGHHFETALSVEAEGR
                     HPIRRLGNELQGAAFGEGWGLYSERLAHEMGLYADEYERLGMLEMQSLRAARLVVDTG
                     IHAFGWERERAVGELEGTGLPRWMAEAEADRYIAMPGQALCYKVGQLEIDRLRVEASR
                     RSGFRLADFHDRLLEVGSVPLPTLRRELAVAGDA"
     gene            3471..3620
                     /locus_tag="VF097_07040"
     CDS             3471..3620
                     /locus_tag="VF097_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011222411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEX6262583.1"
                     /translation="MAMVTDPVCGMRIDPEDAAATAEHEGTTYSFCSEACHEAFVEDP
                     ESYVS"
     gene            3624..4835
                     /locus_tag="VF097_07045"
     CDS             3624..4835
                     /locus_tag="VF097_07045"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6262584.1"
                     /translation="MPPDRISEAELAERAGTSVEHVRELAELGIVEPKAGTFPRRDVM
                     RVRVVLELEAKGMDAASLGAAVAGGHLTLGYLESAGRRFPRSDRTLEELGEEIGIPLE
                     TLQAAYVASGLSRPAADELVREEDLPVLRALPVLFGAGVGEGEVLRAVRVWGDSARRV
                     AQFQTHYFHHTIEEPFRRRGLLDNEAFEAAIREVGLRMGHSGEQMLGWLFRRHSERFL
                     TEHQFEHVEAALEEAGVRSRTPKGAEAVVFADLSGYTKLTEEAGDEVAARVSISLAQL
                     VNEVASRHDGEVVKMLGDGVLFHFREPEDSARASLEIVEAVRPRGLPPAHVGINAGPM
                     IYDEGDYFGRTVNIAARIASEASADRVFVGEDARREMRPSGFRLVEVGPFSLKGIAEP
                     VTIYEALPDAE"
     gene            4909..5280
                     /locus_tag="VF097_07050"
     CDS             4909..5280
                     /locus_tag="VF097_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018639109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6262585.1"
                     /translation="MSVRVLIVDDQEPFRMAARAVVELTDGFEVVGESETGEAAVEAA
                     RGLQPDLVLMDVNLPGIDGLEATRRILAEAAGRVIVLMVSTYEAEEYAPRAAEVGAAA
                     YIPKSEFEPDRLEEAWAAATG"
     gene            complement(5214..7313)
                     /locus_tag="VF097_07055"
     CDS             complement(5214..7313)
                     /locus_tag="VF097_07055"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX6262586.1"
                     /translation="MKRRSAARLAWSLAAVAVALSVVGIALAVAGRRDVGFNLAVLLV
                     GVAPFMSVGALVASRFPRNPIGWIFCAVGLLQALQVASYEYAVEAVIERDGALPLGGL
                     AAWISALAWMPSLGLLTTYLFLLFPDGHPPSPRWRWVGWTGGIGLGAVVVAAMVGNAT
                     GTPRALLSEEPLEYPPLVGALLLLGFVTVLVASVASVASLVVRYRRSRGDEREQLKWV
                     AFAATFALVAIAVQFVPVGWPDWFQLLVGLGFLAVPVASGVAILKHRLYDIDVVINKT
                     VVYGALAAFVTVVYVGIVAGIGSLVGSGGNAFLTIVATAIIALLFQPARVRARHFANR
                     LVYGKRATPYEVLSRLAQGMGSQYSADDALPSLARVLGEGTGAARAEVWLRVGSDLRP
                     AASWPDDVEPRQRSIALADGDLPPIVGADLTLPVKHEGEVLGALAISMPRGETVTPDT
                     ERLLADVASQAGLLLRNVRLIEELRASRQRLVAAQDEERRRLERNIHDGAQQQLVALS
                     VKVRLAESLARKDPDKATELMAEVRAETQQALDDLRDLARGVYPPLLADKGLAAALEA
                     QARKVPFPVRVEPNGIGRYPPEAEANAYFCVLEALQNAAKYAAASAAVVRLFEDAGHL
                     QFVVSDDGTGFDPDVVPRGSGLQNMADRLEALGGRLEIASSSGRGTTVTGRIPVQPVA
                     AAQASSSRSGSNSDLGM"
     gene            complement(7310..9391)
                     /locus_tag="VF097_07060"
     CDS             complement(7310..9391)
                     /locus_tag="VF097_07060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX6262587.1"
                     /translation="MKSRISARIAWSIVVLATGLAGIAIALQVLREGAGLGLVENLLF
                     AAIFISMGSVGALVVGGEPRNAVGWILLGVAVLPALAFFAEMYARYGQTVAAGSLPAL
                     AWGWWLGDWTWMGLLAMIATLPLVFPDGRPPSARWRPYLWLVLSMALITAVLFALDPA
                     AYPGGRHTNPIGVEALRPVADFVEGPGYLLFVGLFLGSVASLIFRFRRAGREQRQQIK
                     WFLFAVAVLVTLFVLDGVFQALGLSDLEGVSFWVNTVLSAAAFASLPVGLGIAILRHR
                     LYDIDVVISKTVVYGALAAFVTLVYVGIVVGIGALVGTRGNLFLSIVATAVIALAFQP
                     VRRWAGHLANRLVYGKRATPYEVLSRLAQGMGSQHTADDALPSLAQVLAEGTGAARAE
                     VWLRVGGALRLAAAWPDVGSQVETIAILDEGLPEIRTVEAVVPVEHDGDLLGALGISP
                     ARGEALTATTEKLLADVGAQAGLLLRNVRLIEELRASRQRLVTAQDEERRRLERNIHD
                     GAQQQLVALSVKLRLIEAMIEKDPAKARELAAETKAESQEALDNLRDLARGIYPPLLA
                     DKGLAAALEAQAHKAPFPVTVEPNGVGRYPAEAEATAYFCVLEALQNVSKYADASGTT
                     VSLSDEDGQLVFSVADDGRGFDPRSTPSGSGLQNMRDRVEALGGRLEIRSAPGQGTTL
                     IGRVPTSGARR"
     gene            complement(9512..10186)
                     /locus_tag="VF097_07065"
     CDS             complement(9512..10186)
                     /locus_tag="VF097_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6262588.1"
                     /translation="MALRIVFAEDDYLVREGTAALLSQAEDVEVVAIVADRDALLAAV
                     GEHEPDAVLTDIRMPPTRTTEGIDAARTIRAEHPDTGVVVLSQYAEEEYAYELLKDGA
                     EGLGYLLKERVADIEEVVRALHDVAQGGSVLDPKIVEGLVARRSREERSPLGRLTERE
                     REVLALMAEGKNNAAIAASLYVTERAIEKHINSLFHKLGLSEEPHVHRRVMAVLTFFR
                     EGPTAP"
     gene            10210..11487
                     /locus_tag="VF097_07070"
     CDS             10210..11487
                     /locus_tag="VF097_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6262589.1"
                     /translation="MTIRVVLGDDNVLIREGVRALLDATDGIEVVGVAEDAPTLLAAA
                     DEHRPDVVVTDIKMPPHFQLEGIEAAHEIRRRSPDTGVVVLSSHDDEAYALALLGEGQ
                     SGLAYLLKDRLGEGDELPRAIREVAAGGSAVDPTIAERLSGRQPDEADREILDLMAEG
                     LGYEAMAERLGTTQEAIDRRVTELFRRLSTEVGRGASHAVDQLKRLHAAVVDRETDAR
                     TLRSFVPSQVAERLRGSASADLQEEVEATILFSDIRGFSSLAETMEPRDLAELLAKHL
                     AAMSEVVLEHGGMIDKFAGDAVMAVFGVPDHQPDHAERGLRCALAMQARQAEINAESD
                     VPLGVGIGVNTGIVMAGTVGGGGRLEYTVLGDAVNVAQRLQGVAAAGEVIAAAATVTA
                     APGIPAEHVGAQEVKGRSEPVEVYSVGERSDGA"
     gene            11477..11860
                     /locus_tag="VF097_07075"
     CDS             11477..11860
                     /locus_tag="VF097_07075"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6262590.1"
                     /translation="MVRELRFCLVVDDYEAAARLYRDVLGLEVLLDLDDQGGRGVILK
                     VPEATLEIVDPEHGAMVDEIEVGRPLREPVRIAIRVDDLEEASRAVEAAGAEPLAGPV
                     DTPWGDRNRRYRGGEGFQLTLFQTG"
     gene            complement(11911..12363)
                     /locus_tag="VF097_07080"
     CDS             complement(11911..12363)
                     /locus_tag="VF097_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262591.1"
                     /translation="MKERFTEEEWRGIMALPFAIFGVVAGADGTIDEQEVGEFTKRML
                     NPTWTYKDAMHQELAVAVAGEDISVLIKEGMALDPEPVKTALKEKLTQDEYQSFLGSV
                     FIDELAVARASGGGIFHKEKIGDEETEKLVGFAAYFGIDLPSVQRIFS"
     gene            complement(12489..12965)
                     /locus_tag="VF097_07085"
     CDS             complement(12489..12965)
                     /locus_tag="VF097_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011904993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX6262592.1"
                     /translation="MPAPAGGGADGSEDEAVFDSPSGWVGRHVREYVETNGRKGHRWS
                     GVHTLLLTTRGRKSGKLRRTALIYGRDGDRYLVVASKGGAKSHPSWYLNLREHPEVRV
                     QVGPERFQGLARTATPEEKPRLWRIMTEIWPEYDRYQERTARDIPVVILEPAGESA"
     gene            complement(12937..13257)
                     /locus_tag="VF097_07090"
     CDS             complement(12937..13257)
                     /locus_tag="VF097_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262593.1"
                     /translation="METRPGQTRRRRGLGATLVLVASLALLLSSCAAGPNPAVGTGEP
                     AGFLLGLWHGIILPVTFIISLFTDDVSIYEVVNNGNWYDFGFILGVLISLGGGGAGAG
                     RRRG"
     gene            complement(13341..13904)
                     /locus_tag="VF097_07095"
     CDS             complement(13341..13904)
                     /locus_tag="VF097_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262594.1"
                     /translation="MRSVSDGLLLTIAQIAATLMGLLMVAVFFYVESGFRRLRTLGEE
                     ANRYMRATTKVVLMLYALVLALSLALAMLEDRWASLVLAVMSGAVVLSLAQWTSRSRE
                     LRRTVRIRGTSAWGGWVLTLIPLLLPWAVDGWIPGRETLTIALLLVGAFAFANSIGLL
                     LVAFDLADLEKAAEAAAHRERQASEER"
     gene            14249..14707
                     /locus_tag="VF097_07100"
     CDS             14249..14707
                     /locus_tag="VF097_07100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEX6262595.1"
                     /translation="MYARHSSDAFRLAYLVTGDQPLAEDLVQEAFINVAGRLVHLRHP
                     DAFPAYLRRTVLNLCKMHFRRRQVERAYLQREGHRIEDDDPTLASDLREDLWSELQRL
                     PHRQRVAVVLRYYEGLTDHEAAEILECRPGTVRSLLSRAMSVLRTRVRTG"
     gene            14709..15326
                     /locus_tag="VF097_07105"
     CDS             14709..15326
                     /locus_tag="VF097_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262596.1"
                     /translation="MESHENEVEASLREMLRQKAGTLPPPRGLSAPALRRTRLRIART
                     ITTAALVLGLSGFAATAALDRLTGTSEVTPGGASFVQGATVRIRFDGSLGDLGCSHDG
                     PRTVSAREEVTFGLTNDGRVDIVVDVLRFAEGKGFDDLVAYVEDPREDPEVAPEWATS
                     QHAFRASPGQRESWTRALTPGEYGVVCYTTDPPRLWLLSPLTVAD"
BASE COUNT         2354 a         5283 c         5519 g         2218 t
ORIGIN      
        1 gagggcaact tcgactggat cgagccggac gacgagttcc acgcgttcat caacgacctg
       61 cagcggccgg tcggcaccta cctctacggg cgcaggatct acgaggtgat gtcggtcttt
      121 gagtccgacg aggtgttcga gagcgtgtac gccgagcagg gcgtggaggt atctcccgcg
      181 gtccgtgact tcgccagcat ctggcgggcg gcggagaagg tcgtctactc gcgcacgctc
      241 gaggaggtga cgaccgcgag aacccggctg gagcgcgcgt tcgaccccga ggcggtccgt
      301 gagctgaagg cgagctcagc ggccgacctc tcgatcgccg gccccgagct cgcagctcac
      361 gcgcttcgcg ccggcctcgt cgacgagctt cagctgaccc tgtctccggt cgccgtcggg
      421 ggcggcaagc gcgcgctgcc ggacggcctg cgcatcgacc tcgagctgac cgacgagctc
      481 cgcttcgcga gcgggatcgt ctacctcggc taccgcgtgc tcgccggtga ctaggtcgac
      541 cacgagagcc gatccatccg ggcccgcgcg agtgaatcga gaaacgttct gaccgagcgc
      601 gaggggctcg agtagtgccc gctcgccgac gtgtccgaac ccgaatcaac cggtcctgcg
      661 ctcggcacct gggcgagcgt tccagtcgtt cgggctcgcc gcgcccgcag cttcccgcac
      721 ggcgccttgt ttgagtcggg gtcgcccgtg ggatggagcg cgtgggatgg gcccgaacca
      781 aaaggaggca gacatgcggg tcatgatgat ggtgaaaggg gaccaggagc ccggagagat
      841 ccccagcgag aagctcctgg cggcgatggg cagctacaac gaggagctgg cgaaggccgg
      901 cgttctgctc gacctcgccg ggcttcatcc cagcgcggag ggtgtccgcg tcaggctctc
      961 cggcgggaag accaccgtgg tcggcgggcc cttcgctgac cggagggaga tcgtcgcggg
     1021 ctactggctc ctgcaggtga ggtcgatgga cgaggccatc gagtgggcca agcgtctccc
     1081 ggtcgaggcg gccgagcacg actacggcca ggagagcgag atcgagatcc gccaggtctt
     1141 cgagctcgag gagttcgctg agggcccggc gatcgaccgg gccggcggcc tcgaggagga
     1201 gtagttggga gtcaggggct ccctacctct cgatggacca tcgcgactga tccatctggg
     1261 agtatgctcg ctccgatccg tcggaccggg agtgggggat gagccagccg agcaaccaga
     1321 cgatcgtgca gcgctacatg gaagcgttcc cgatggactt cgacgttctc ggggagctcc
     1381 gtcatcccga ctacgtcgag gactggccgc agtcgggcga gcgcatccgc ggccacgagc
     1441 gctacgtcag gatccacgag cagtacccgg gaggcctccc cgagtccgag gttcgccgga
     1501 tcgtggggag cgaggaccga tgggtcacga cgccctcgtt caccctgctc cggatcaacg
     1561 ggacggggga caccttcacc gtcgagggga cgctgaccta cccgaccggg aacgccaccc
     1621 acctcgtggc gatcctccgg ctctgggatg gaaaggtggc ggaggtcacc cactacttcg
     1681 cggacccgtt cgaggcgccg gactggcggg cggagtgggt cgagcccatc ccctagcagg
     1741 caccggcgcc ggggcggcgg cgcggtagcc tgggcggccc cggcggacga gaaagggacg
     1801 aggatgggac ctgtggagcc gcgcgagctg ctcgatcgct tctgggacga gttcctgggc
     1861 ctggagccgg tcttcgcaac cctggtgggg gacgagcggt tcgacgatcg cctcccggac
     1921 ccgagcccgg agggcctggc ccgccgcgag tccgtccatc gttcgacgct ggaagccctg
     1981 ggggacgtcg accgcaccgg cctcgggatg gaggagcgga cggcgctcga catggccgag
     2041 tcgctggcct gccgggacct ggcgatcgtc gagctccgcc tggaccgcct ctgggcggtg
     2101 agccacatgc agggcagcta cctgtgcggg ccgggcctgc tcccaggcca cctgggggcg
     2161 ctccagcgcg cggacactcc cgagcgggtg gaccgctacc tggcacggct gtccgccgtc
     2221 cccgcctatc tcgaggcgat ccggggcgtc atgcgcgagg cgatcgacgc cggtcaggtg
     2281 gcgccggccg tggtcgtgga ccgctcgatc gggctcgtgg agcggcagct cgaggcaggg
     2341 gccgaggcct cgcccgcgat gcagcccgcg acggaggggg cggaccgcga tcgggtggcc
     2401 gccgtcctgg ccgacgtcgt gctccccgcg tacgcggact acctggaagc cctccgcgcg
     2461 taccggccct cggccaggga gacgctcggg cttcacgcgc tccccggagg ggaggagatg
     2521 tacgcggcgg agatcctcgg gtggacgggc ctcccgctcg acacgggaga gcttcacgag
     2581 ctgggacgct cccagctcga ctcgatccgc gcggagggag cggccatcgc ctcgggcctc
     2641 ggtttcccgg acccggccga ggcgatcgcc gcccgcgccg accacgagat cgcgcgagat
     2701 gccgtccttc tggtggctcg ggagcaggtg gagcgcggat gggaggccgc gaaagggttc
     2761 ttcggtcggc ttcccgctcg gaactgcgag gtccgcgccg tgccaccgga cctggaggag
     2821 gacgccctcg actactacca ggggccgacc gacgactggt cccgcccggg ggtctactgg
     2881 gtcaacacgg cgccccgtcc ccggcacagc ctcgcgccca ccacctacca cgaggccacc
     2941 ccgggccatc atttcgaaac ggccctctcc gtggaggcgg aggggcgcca tccgatccgc
     3001 cggctgggca acgagctcca gggagccgcc ttcggcgagg gctggggcct ctacagcgag
     3061 cgcctggccc acgagatggg cctctatgcg gacgagtacg agcgcctggg catgctcgag
     3121 atgcagtccc tccgcgcggc ccgtctcgtg gtcgacaccg ggatccatgc gttcggatgg
     3181 gagcgcgagc gagcggtggg tgagctcgag ggcacggggc tcccgcgctg gatggcggag
     3241 gcggaggccg atcgctacat cgcgatgccg ggccaggcgc tctgctacaa ggtgggccag
     3301 ctcgagatcg atcggctgcg cgtggaggcg tcccggcggt cggggttccg cctggccgac
     3361 ttccacgacc ggctgctcga agtgggctcg gtgccgctcc cgacgctccg ccgcgagctc
     3421 gccgtcgccg gcgacgcgta gaatccactg actcgacgaa gggggatccc atggccatgg
     3481 tgaccgatcc ggtctgcggg atgcggatcg acccggagga cgcggcggcg accgccgagc
     3541 acgaggggac cacgtactcc ttctgctcgg aggcctgcca cgaggcgttc gtggaggatc
     3601 ccgagtcgta cgtgtcctga ggcatgcccc cggatcggat cagcgaagcg gagctcgccg
     3661 agcgtgcggg gaccagcgtc gagcacgtgc gggagctcgc cgagctcggg atcgtggagc
     3721 ccaaggccgg gaccttcccc cggcgggacg tcatgcgggt gcgggtcgtc ctggagctcg
     3781 aggcgaaggg gatggacgcg gcatccctgg gcgcggcggt ggcgggcggc cacctgacgc
     3841 tcgggtacct ggagagcgcc gggcggcggt ttccgcggtc cgatcgaacg ctggaggagc
     3901 tcggcgagga gatcggcatc cccctcgaga cgctgcaggc ggcctacgtg gcgtccggtc
     3961 tctcgcggcc cgctgcggac gagctcgtgc gcgaggagga cctccccgtc ctccgagcgc
     4021 tcccggtgct gttcggggcg ggcgtggggg agggcgaggt cctccgcgcg gtgcgggtct
     4081 ggggcgacag cgcccgccgc gtggcccagt tccagaccca ctacttccac cacacgatcg
     4141 aggagccgtt ccggcgtcgg gggctcctcg acaacgaggc cttcgaggcc gcgatccgcg
     4201 aggtcgggct tcggatgggg cactcgggtg agcagatgct gggatggctg ttccgtcgcc
     4261 actcggagcg gttcctgacg gagcatcagt tcgagcacgt ggaggcggcg ctcgaggagg
     4321 ctggcgtccg ctcgcggacg ccgaaggggg cggaagccgt ggtgttcgcg gacctctccg
     4381 gctacacgaa gctcaccgag gaggccggcg acgaggtcgc ggcccgcgtc tcgatctccc
     4441 tcgcccagct tgtgaacgag gttgcgtcac gccacgacgg ggaggtcgtg aagatgctcg
     4501 gcgacggggt cctcttccac ttccgagagc cggaggactc ggcccgggcg tcgctcgaga
     4561 tcgtggaggc cgtgcgtccg cgtgggctac cgccggctca cgtcgggatc aacgcgggcc
     4621 cgatgatcta cgacgagggg gactacttcg ggcgaacggt gaacatcgcc gcccggatcg
     4681 cctcggaggc gagcgccgac cgggtgttcg tgggggagga cgcccggcgc gagatgaggc
     4741 cctcgggctt ccggctggtc gaggtgggcc cgttctcgct gaagggtatc gcggagccgg
     4801 tcacgatcta cgaggccctc cccgacgccg agtagggctg ccccttccgt ccgaggtggt
     4861 gcccgcatcc ttcccggtgc gtggtcgctg tgagacgctg agactgagat gagcgtgcgc
     4921 gtgctgatcg tcgacgatca ggagcccttc cggatggcgg ctcgcgcggt cgtggagctc
     4981 acggacgggt tcgaggtcgt gggcgagtcg gagacgggtg aggcggccgt cgaggcggcg
     5041 cgtggcctcc agcccgacct cgtgctgatg gacgtcaacc tgcccggcat cgacgggctg
     5101 gaggccacgc gccgcatcct cgccgaggcc gccggtcgcg tgatcgtgct gatggtgtcc
     5161 acctacgagg ccgaggagta cgcgcccagg gccgccgagg tgggggcggc cgcctacatc
     5221 cccaagtcgg aattcgagcc ggaccggctc gaggaggcct gggcggcggc caccggctga
     5281 accgggatcc gccccgtcac cgtcgtgccc cggccggagg acgacgcgat ctcgagcctc
     5341 ccgccgagcg cctcgaggcg gtcggccatg ttctggagtc ccgatcccct cggcaccacg
     5401 tccggatcga atcccgtccc atcgtcggac acgacgaact gcaggtgtcc ggcatcttcg
     5461 aacaggcgga cgaccgcggc ggaggccgcc gcgtacttag cggcgttctg gagcgcctcc
     5521 aggacgcaga agtaggcgtt ggcctcggcc tctggagggt atcgcccgat cccgttcggc
     5581 tccacccgca cggggaacgg caccttgcgg gcctgggcct ccagcgctgc ggccaggccc
     5641 ttgtcggcca gcagcggcgg ataaacccct ctggccaggt cccggaggtc gtccagtgct
     5701 tgctgggtct cggcccgcac ctccgccatg agctcggtgg ccttgtccgg gtccttcctc
     5761 gcgaggctct ccgccaggcg caccttcacc gagagcgcca ccagctgctg ctgcgcgccg
     5821 tcgtggatgt tccgctccag ccggcggcgc tcctcgtcct gggcggcgac cagccgctgc
     5881 ctggatgccc gcagctcctc gatcagacgg acgttccgaa gcagcaaccc ggcctgtgac
     5941 gcgacgtcgg ccaggagcct ctcggtgtcc ggggtcacgg tttctcccct aggcatcgag
     6001 atcgccagcg cgccgaggac ttctccctcg tgcttcacgg gaagggtcag atccgccccg
     6061 acgatcgggg gtaggtctcc atcggccaga gcgatggacc gttgccgagg ctccacatcg
     6121 tccggccagg aggccgcggg tcgcaggtcg ctcccgactc ggagccagac ttcggcacgg
     6181 gcggctccgg tcccttcgcc caggactctg gccaggctcg ggagggcgtc gtcggccgag
     6241 tactgcgagc ccatcccctg ggccaaccgg gacagcacct cgtagggcgt ggcccgcttc
     6301 ccgtacacca ggcggttggc gaagtggcgg gcccggaccc gggccggctg gaacagcagg
     6361 gcgatgatcg ccgtggccac gatggtgagg aacgcgttcc ccccgctccc caccagggat
     6421 ccgatgcccg ccacgatgcc cacgtacacg accgtcacga acgcggccag cgccccgtac
     6481 accacggtct tgttgatgac gacgtcgatg tcgtagaggc ggtgcttgag gatggcgacc
     6541 ccggaggcca ccggcacggc caggaatccc agaccgacca gcagctggaa ccagtccggc
     6601 catcccaccg gcacgaactg gacggcgatc gcaaccagcg cgaacgtggc cgcgaaggcc
     6661 acccacttga gctgttcgcg ctcatctcct cttgatcgcc ggtatcgaac caccagtgag
     6721 gcgaccgacg cgaccgacgc tacgagcacg gtgacgaacc cgaggagcag caacgcgccg
     6781 accagcggcg gatactccag tggctcctcg gagagcaggg cacgaggggt tcccgtggcg
     6841 ttccccacca tcgcggcaac cacgacggca ccgagtccga tcccgccggt ccatcccacc
     6901 catctccatc gcggcgacgg ggggtgtccg tccgggaaca gcaggaacag gtacgtggtg
     6961 agcagcccga ggctcggcat ccacgccaac gccgagatcc atgcagccag accgcccagg
     7021 ggaagcgcgc cgtcccgctc gatgaccgcc tcgactgcgt actcgtacga cgccacctgg
     7081 agagcctgga gcagccccac ggcacagaag atccatccga tcggattccg ggggaaccgg
     7141 gaagcgacca gggcgccgac cgacatgaac ggggccacgc cgacgagtag gaccgcgagg
     7201 ttgaacccga cgtcgcgtcg tccggcgacc gccagcgcga tgccgacgac gctgagcgcg
     7261 acggcgactg cggcgagcga ccacgccagc cgagcggccg atctccgctt caccggcgcg
     7321 ctcccgaggt cggaacccgc ccgatgagcg tcgtcccctg tccgggcgcc gaccggatct
     7381 cgagtcgacc gccgagcgcc tccacgcgat ccctcatgtt ctggagcccg gaacctgacg
     7441 gcgtcgagcg ggggtcgaaa ccccgtccgt catcggcgac cgagaacacg agctgaccgt
     7501 cctcatcgga cagcgagacc gtcgtccccg acgcatccgc gtacttggac acgttctgca
     7561 acgcctcgag cacgcagaag taggcggtgg cctcggcctc ggccgggtag cggcccaccc
     7621 cgttgggctc gaccgtcacc gggaagggcg ccttgtgggc ctgggcctcg agggccgccg
     7681 ccagcccctt gtcggccagc aggggagggt agatcccgcg ggccagatcc cgcaggttgt
     7741 ccagcgcctc ctgcgactcg gccttcgtct cggctgcgag ctcccttgcc ttcgccgggt
     7801 ccttctcgat catcgcctcg atcagcctga gcttcacgga cagggccacg agctgctgtt
     7861 gcgccccgtc gtggatgttc cgctccaggc gccgccgctc ctcgtcctgg gcggtcacaa
     7921 gccgctggcg tgacgctctc agctcctcga tcagccggac gttccgaagg aggagccccg
     7981 cttgggcccc gacgtcagcc aggagcttct ccgtggtggc cgtcagggcc tctccccgag
     8041 caggcgagat cccaagcgcc ccgaggagat cgccgtcgtg ctcgaccggc acgaccgcct
     8101 cgaccgttcg gatctctggc aggccttcgt cgaggatggc gatcgtctcg acctgtgacc
     8161 cgacgtccgg ccacgcggcg gcgagacgga gcgcaccgcc gacccggagc catacctcgg
     8221 cacgagccgc gccggttccc tcggcgagga cctgcgccag gctggggagg gcgtcgtccg
     8281 cggtgtgctg ggatcccatg ccctgcgcca ggcgggagag gacctcgtac ggcgtggccc
     8341 gcttcccgta gacgaggcgg ttcgcgaggt gcccggccca ccgccgcacc ggctggaacg
     8401 ccagggcgat cacggccgta gccacgatgg agaggaagag gttgccccgg gtgccgacga
     8461 gggctccgat ccccacgacg atccccacgt agacgagcgt cacgaacgcg gccagggctc
     8521 cgtagacgac cgtcttgctg atgacgacgt cgatgtcgta gaggcggtgc cgcaggatgg
     8581 cgatgccgag tccgaccggg aggctggcga acgccgccgc ggacagcacg gtgttgaccc
     8641 agaaggaaac cccctcgagg tcggacagac cgagagcctg gaagacgccg tcgagcacga
     8701 agagtgtgac gaggacggca accgcgaaga ggaaccactt gatctgctgc cgctgttcac
     8761 gaccggcgcg gcggaagcgg aagataagcg acgcaacaga gcccaggaag aggccgacga
     8821 agagcaagta tccgggccct tcgacgaagt ccgccaccgg tcggagcgcc tccacgccga
     8881 tcgggttcgt gtggcgaccg cccggatacg cggccggatc cagggcgaac agcacggccg
     8941 tgatgagcgc catcgacagg accagccaca ggtagggtct ccaccgcgct gaggggggcc
     9001 ttccgtcggg gaacacgagc ggcagcgtgg cgatcatcgc caggagcccc atccacgtcc
     9061 agtccccgag ccaccagccc caggcgagcg cggggaggga gccggcggcc accgtctgtc
     9121 cgtagcgtgc gtacatctcc gcgaagaagg ccagggcggg caggactgcc accccgagga
     9181 ggatccatcc cacggcgttc cggggttccc cccccaccac gagagctccc acggagccca
     9241 tcgagatgaa gatcgcggcg aagaggagat tctcgacgag cccgaggccc gccccttccc
     9301 ggagtacctg cagcgcgatg gcgatgccgg ccagccccgt ggccagcacg acgatcgacc
     9361 atgcgatccg cgcagagatt cgggatttca cgtcccagat tgtgccctgc gacggagacg
     9421 cgggaaaggg gggcttcccc acgtcgggga agggtgcttc caccactccg ctccacatgg
     9481 agccgcgctt ggaccggtcg cccgggtcgg tctagggtgc cgtcggacct tcccggaaga
     9541 aggtgaggac ggccatgacc cggcggtgga cgtggggctc ctccgagagc ccgagcttgt
     9601 ggaagaggga gttgatgtgc ttttcgatgg cgcgctcggt gacgtagagc gacgcggcga
     9661 tggccgcgtt gttcttcccc tcggccatga gggcgagcac ctcccgttcc cgctcggtga
     9721 ggcggccgag tggcgaccgc tcctcgcggg agcggcgggc cacgagcccc tccacgatct
     9781 tcgggtcgag gacggagccg ccctgggcca cgtcgtggag ggcccggacc acctcctcga
     9841 tgtcggccac ccgctccttc aggaggtacc cgaggccctc ggcgccgtcc ttcagcagct
     9901 cgtaggcgta ctcctcctcc gcgtattggg agaggactac gacgcccgtg tccgggtgct
     9961 ccgcgcggat cgtccgggcg gcgtcgatcc cctcggtggt cctggtgggg ggcatccgga
    10021 tgtcggtgag gacggcgtcg ggctcgtgct cgcccacggc ggccaagagc gcgtcgcgat
    10081 cggccacgat ggcgaccact tccacgtcct cggcctggga caggagcgcc gccgtcccct
    10141 cgcgcacgag gtagtcgtcc tccgcgaaga cgatgcgcag ggccacccgc ggatggtaat
    10201 acgggagccg tgacgatccg cgttgtgctc ggggacgaca acgtcctgat ccgagagggg
    10261 gtccgagcgc tgctcgacgc caccgacgga atcgaggtcg tgggcgtggc cgaggacgcc
    10321 cccacgctgc tcgccgcagc cgacgagcat cgccccgacg tcgtggtgac cgacatcaag
    10381 atgccccccc acttccagct cgagggcatc gaggccgccc acgagatccg ccgccgctcc
    10441 ccggacaccg gggtcgtcgt cctctcgtcg cacgacgacg aggcctacgc tctggccctc
    10501 ctgggagagg ggcagagcgg gctggcgtac ctcctgaagg accggctggg ggagggggac
    10561 gagctgcccc gggccatccg ggaggtggcc gcggggggct cggcggtcga cccgacgatc
    10621 gcggagcgcc tgtcgggccg tcagccggac gaggccgatc gggagatcct cgacctcatg
    10681 gccgaagggc tgggctacga agccatggcc gagcgcctgg gaaccacgca ggaggcgatc
    10741 gaccggcgag tgacggagct cttccggcgc ctctccaccg aggtggggag gggggcctcc
    10801 cacgcggtgg accagctcaa gcggctccac gccgcggtgg tcgaccgcga gaccgacgcc
    10861 cggaccctcc ggtcgttcgt gccctcccag gtcgcggagc ggctccgagg gtcggcttcc
    10921 gcggatctcc aggaggaggt cgaggcgacg atcctcttct ccgacatcag gggcttctcc
    10981 tcgctggccg agacgatgga gcctcgggac ctggccgagc tgctggcgaa gcatctcgcc
    11041 gcgatgtccg aggtcgtgtt ggagcacggc gggatgatcg acaagttcgc cggcgacgcg
    11101 gtgatggcgg tgttcggcgt cccggaccac cagcccgatc acgccgagcg gggcctccgc
    11161 tgcgccctgg ccatgcaggc tcggcaggcg gagatcaacg cggagtccga cgtccccctc
    11221 ggggtgggga tcggcgtgaa caccgggatc gtgatggcgg ggaccgtagg cgggggcggc
    11281 cgcctcgagt acacggtcct gggcgatgcg gtcaacgtcg cccagcgcct ccagggcgtg
    11341 gcggcggcgg gcgaggtgat cgccgccgcc gccacagtga ccgcggctcc cggcatcccc
    11401 gccgagcacg tcggagccca ggaggtcaag ggccgtagcg agccggtcga ggtctacagc
    11461 gtgggagaga ggagcgatgg tgcgtgagct tcggttctgc ctggtggtgg acgactacga
    11521 ggccgccgcc cgcctctacc gggacgtcct cgggctcgag gtgttgctgg acctcgacga
    11581 ccagggcggc aggggagtga tcctgaaggt cccggaggcg acgctcgaga tcgtggatcc
    11641 cgagcatgga gcgatggtag acgagatcga ggtggggcgc cccctccggg agccggtgcg
    11701 gatcgccatc cgggtggacg acctcgagga ggccagcagg gcggtcgagg ccgcgggggc
    11761 cgagccgctg gcgggcccgg tggacacgcc gtggggcgac cggaaccgcc gctaccgcgg
    11821 aggggagggg ttccagctca cgctgttcca gacggggtga gggccggtgg ccgagggatc
    11881 agccagatcc ctcgaccacc ggccccccga tcacgagaag atccgctgga ccgacggcag
    11941 gtcgatcccg aagtaggccg cgaagcccac gagcttctcg gtctcctcgt ccccgatctt
    12001 ctccttgtgg aagatcccac ccccgctggc ccgagccacg gccagctcgt cgatgaagac
    12061 gctgccgagg aagctctggt actcgtcctg ggtcagcttc tccttcagcg ccgtcttcac
    12121 cggctccgga tcgagggcca tcccctcctt gatcaggacg ctgatgtcct cgccggccac
    12181 ggccactgcc agctcctggt gcatcgcgtc cttgtaggtc cacgtggggt tcagcatgcg
    12241 cttcgtgaac tccccgacct cctgctcgtc gatcgtgccg tcggcgcctg cgacgacgcc
    12301 gaagatcgcg aacgggagcg ccatgatccc gcgccactcc tcctccgtga atcgctcctt
    12361 catactggtt cgcccccttc catccgtccc ccagcgtagt gacggctcga gaggagccac
    12421 aggggagggg gtcccgggtc ggaggggtcc atcggctccg gcccgggctg gtcactcagg
    12481 gtggtcgatc acgccgactc gccggcgggc tcgaggatga ccaccgggat gtcccgggcg
    12541 gtccgctcct ggtagcggtc gtactccggc cagatctcgg tcatgattcg ccagaggcgg
    12601 ggcttctcct cgggcgtggc cgtccgcgcg agcccctgga acctctccgg gcccacctga
    12661 acgcggacct ccggatgctc gcgcaggttg aggtaccagg agggatggct cttcgcgccg
    12721 cctttcgagg ccaccacgag gtagcggtcg ccgtcccgcc cgtagatgag cgcggtccgc
    12781 cggagcttcc cggacttcct gccgcgcgtg gtcagcagca gggtgtgcac gccggaccag
    12841 cggtggccct tgcgcccgtt ggtctccacg tactcgcgga cgtgtctgcc gacccagccg
    12901 ctcgggctgt cgaagactgc ctcgtcctcc gagccgtcag cccctccgcc tgccggcgcc
    12961 ggcaccgccg cctccgagag agatcagcac gccgaggatg aacccgaagt cgtaccagtt
    13021 cccgttgttg acgacctcgt agatgctcac gtcgtcggtg aagagggaga tgatgaacgt
    13081 gacggggagg atgatcccgt gccagaggcc caggaggaac ccggccggct cgcccgtccc
    13141 gaccgccggg ttggggcccg ccgcgcacga cgagaggagc agcgcaaggg acgccaccag
    13201 cacgagggtc gcgccgagtc ctcggcgcct gcgcgtctga cccggcctgg tctccatccc
    13261 ggcgattctg acacgggccg gagcgagccc gccacccgtg cgtcagccct cgagcggggc
    13321 ctatggcatc cgggacccgg tcaccgctct tctgaagcct gcctctcccg gtgagcggcg
    13381 gcctcggcag ccttctccag gtccgcgagg tcgaaggcga ccagcaggag cccgatgctg
    13441 ttggcgaacg cgaacgcgcc cacgagcagc agcgcgatcg tgagggtctc ccggccgggg
    13501 atccacccgt ccacagccca cgggagcagg agcgggatca aggtcaggac ccatccgccc
    13561 cacgccgacg tgccgcggat gcgcaccgtt ctgcggagct cacgagagcg cgaggtccac
    13621 tgagcgagcg acaggacgac ggcgccgctc atcacggcga gcacaaggga ggcccatcga
    13681 tcctcgagca tcgcgagggc cagggacagg gccagcacga gggcatagag catcagcacc
    13741 accttcgtgg tggctcgcat gtagcggttc gcctcctcgc cgagggtcct gagccgacgg
    13801 aaccccgact cgacgtagaa gaagaccgcc accatgagga ggcccatcag ggtcgcggcg
    13861 atctgcgcga tcgtgaggag cagtccgtcg gagaccgacc tcatgcctac acctcctctc
    13921 cgtcccgtcc gggcgtggtc tcctatgaga ttccgtggag gcggtgctgg tgtcctgggg
    13981 cgatggtcac gaggagcagg ggtccttcga cccgcttgcc gctgatgccc cgtcgggtcc
    14041 aacggtcgaa ggcggcccgg ccaggcgcaa ccttcgctcc acgcctccgt ccggcggtcg
    14101 ccacgacgaa cggcatcgga gcgacccaac ggtgatccct gcaacacttc cgcgacgtta
    14161 cgcatctcag ttgtgagaag gcaccggtgc ataatggtcg ccagagcttc ggaggtgggt
    14221 cgcggccgag tggggacgcc tgggcgagtt gtacgcgcgc cactcctccg acgcgttccg
    14281 cctggcctac ctggtgaccg gagaccagcc cctggccgag gatctggtgc aggaagcgtt
    14341 tatcaacgta gcgggacggc ttgtgcacct tcgtcatccg gacgccttcc ctgcctacct
    14401 tcgtcggacc gtgttgaacc tctgcaagat gcacttcagg aggaggcagg tggagcgagc
    14461 gtatctgcag agagaggggc atcggatcga ggatgatgat ccgaccctcg catcggacct
    14521 gcgggaggac ctgtggtcgg agcttcagcg gctgccgcac cgccagcgag tcgccgtcgt
    14581 gctccgctac tacgagggtc tcacggacca tgaggcagcc gagatcttgg agtgccggcc
    14641 ggggaccgtc cggtcgcttc tctccagggc catgagcgtc cttcgcacgc gcgtcaggac
    14701 ggggtgagat ggagagccac gagaacgagg tcgaggcgag cctccgcgag atgctgcggc
    14761 agaaggcggg cacactcccc ccaccgcgtg ggctctcggc cccggcgctc cggcgcacgc
    14821 ggctgcgcat cgctcgaacc atcaccaccg cagcattggt gctggggctc tcgggcttcg
    14881 cggcgaccgc cgccctggat cggctcacgg gcacgagtga ggtgacgccg ggtggggcct
    14941 cgttcgtcca gggagcgacg gtccggatca ggttcgacgg cagcctcggc gacctcggct
    15001 gctctcatga cggaccgcgg acggtctcgg cccgggagga ggtcaccttc gggctgacga
    15061 acgacggacg ggtcgacatc gtggtcgacg ttctcaggtt cgccgagggc aagggcttcg
    15121 acgacctcgt ggcgtacgtg gaggatcccc gggaggatcc ggaggtggca cccgagtggg
    15181 ccacctcaca gcacgccttt cgtgcctcgc ccgggcagcg ggagtcctgg acgagggcgc
    15241 tcacacctgg tgagtacggc gtcgtgtgct acacgacgga tccacctcgc ctgtggctcc
    15301 tcagcccgct gaccgtggcc gactgacctc gctccaaggg cgcaacggag cggggagccc
    15361 gcgcatctct ctat
//
LOCUS       DASUTZ010000163        10762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1732879, whole genome shotgun sequence.
ACCESSION   DASUTZ010000163 DASUTZ010000000
VERSION     DASUTZ010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10762
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732879"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..956
                     /gene="dapA"
                     /locus_tag="VF097_07110"
     CDS             78..956
                     /gene="dapA"
                     /locus_tag="VF097_07110"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX6262597.1"
                     /translation="MAGSFGTLVTAMVTPFKDDGSLDLDRARDLAAWLFDHGTNALVV
                     TGSTGEAATLSDGEKVELWRACAEVGGGMIIAGTGTYDTAHSVHLTHQADEAGVDAML
                     VVTPYYNKPPQRGLIEHFTTVAGATSKPVMVYNIPGRTGTRIEHDTLLRLAEVENIVA
                     VKDSTGDLDGVSHLVAQAPTDFELYCGDDWATFAMMCAGARGLVSVAAHVVGERLKRM
                     MELIEGGDAAGARKIHEELLPVFDALFITSNPIPLKAAMEMIGQPVGPPRLPLVPATD
                     EERSRIGKALEDAGVL"
     gene            940..2598
                     /locus_tag="VF097_07115"
     CDS             940..2598
                     /locus_tag="VF097_07115"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX6262598.1"
                     /translation="MQASSSLVFLGGVGEVGRNMACLESNGRVLVLDAGLSFPTEDMP
                     GIDLVLPDWEWLRRRRDRIDGLILTHGHEDHMGAVPYILREIPLRIHATALALALLRP
                     KLEEHGVEDRAELVEVNPGQETRIGSFTARFHRVTHSIPDGVAVAIDIPQGTILHSGD
                     FKLDPTPIDERPTDLQGIADEARRGVHVFLSDSTNAEEKGTIPSERTVGPRLLEIFRR
                     GERLIVAACFASHIHRVQQICDAAREVGRKIAFLGRSMHQSVEQGRRLGYLDVPEEDV
                     IDIERLNDYDPREVAVVCTGSQGEPLSALALMAAREHKWVDLEEGDLVVIAASFIPGN
                     ETAIHRTIDGLYRTGADVFHSKIADVHVSGHAAADELQFMLSLVRPRWFIPIHGERRH
                     LAHHARLARGVGVPSDRILIVEDGDVVEVGAEAVKIVDRAPSGMIFVDGLGIGDVGDV
                     VLRDRRKLAGDGFVVVVVTADAHSGEVVAGPDVITRGFVHDETSVEMLEEAKARTLAS
                     LAEVAEEEVADPSILRQHIRRTLGRYFFEAIRRKPVIVPVIIEV"
     gene            2598..4751
                     /locus_tag="VF097_07120"
     CDS             2598..4751
                     /locus_tag="VF097_07120"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX6262599.1"
                     /translation="MARKRKRRTGGAVSAAGQKVAEHLGPQLTDALGIGLLVLAGLSV
                     LGIWLDAAGPLGRFLTIVVVGMLGLAGYAFPVLALYWGLLLVRGTAEEDRGRMFVGLI
                     FLLMGGLGIVSLVRGNPSPAAGFSGFGDAGGLVGAVTAWPLSKVMSVYGAAIVCIGLV
                     ALGALVFTATPLRSVWEWLVRREDEVLDLTEVEEATPKRRRRKQPEAEGEILTPALPD
                     PEPEEESSPSVVPSPSGRGYKLPPVDMLRKAPASGGPGRDEKETMAILERTLRNFGVA
                     ASVVGASRGPTVTLFEVEVSAGTKVNKVLNLGDDIAYALATPDVRIIAPIPGRSAIGV
                     EVPNKVRDFVTLGDVLGSKAAGKEKHPLAVALGKDVQGNPLLLRLNEMPHLLIAGATG
                     AGKSSLINAFVTSILIRAHPDEVKLMLVDPKRVELSHFADLPHLISPVIVAPKRAAEA
                     LGWVVREMELRYESLATVGMRDIDSYNGAHAEGSLRIPPGRDAEFERLPYILVVIDEL
                     ADLMMVAPRDVEDAIVRISQMARAVGIHLVVATQRPSVDVVTGLIKANIPSRIAFMTA
                     SAADSRVILDVGGAEKLVGHGDLLFLPSSASKAARIQGAWVTQHEIDAVAEYVRQQRE
                     PDYVEGIEGLGKPPADDEDLDAEDDLLQQAMELVIHSQLGSTSMLQRKLKVGFARAGR
                     LMDMLEERGVVGPSVGSKPRDVLITWDEYAESRAG"
     gene            complement(4767..5099)
                     /locus_tag="VF097_07125"
     CDS             complement(4767..5099)
                     /locus_tag="VF097_07125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6262600.1"
                     /translation="MTTRLEARVEAGHRIRLAGELDISTVPELEQSLDGLADDPYDPL
                     VLELTDLTFIDSTGLRAILRLARSHADRGRLVLRAPTPRVLEVLTICGVLGGPSNLEL
                     EREEEGAV"
     gene            complement(5096..5509)
                     /locus_tag="VF097_07130"
     CDS             complement(5096..5509)
                     /locus_tag="VF097_07130"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6262601.1"
                     /translation="MRPGSSAGRRGRSPSSIAAAIRCGVRRSPDPGRLSIEALGRRGL
                     ALIGELDTSARSEVASAIADVVSSPGEVVLDLAGLRFMDSAGIHVLTEAARELKGDDA
                     ITVRHPRPCVLRVLVAAATLGWEGRLRIELDRSEP"
     gene            complement(5530..5880)
                     /locus_tag="VF097_07135"
     CDS             complement(5530..5880)
                     /locus_tag="VF097_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262602.1"
                     /translation="MVSSWRDVEVSNGTVFRRMNEWTHDDTEHLGSRIAAYLCECGDT
                     SCTDPITIHRLKYERVRAYPARFVIALDHENPEIDYVVREAPGFAVVEMVGRSARIAR
                     ATNPRRSHRTGVDR"
     gene            complement(5880..6521)
                     /locus_tag="VF097_07140"
     CDS             complement(5880..6521)
                     /locus_tag="VF097_07140"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6262603.1"
                     /translation="MAQGGHEVGDRLEVRHVAVCDQDPQRRLLWWLHAATLPERADRA
                     HPLTVPPPSRSVGGAGPQPTARHDRALTLGRRSVGDFRRPTERGGLVPDASPTAEPLG
                     VRIQRRNGVVRVLLGGALDLAGARRLAEELESISSDGAEGVLFDARDVTFADRMGLRA
                     MFDALLHLRSEGARTAVIGVTPAVRRLVDLTGSALDGAAGFELLRRFSSAEGG"
     gene            6423..7043
                     /locus_tag="VF097_07145"
     CDS             6423..7043
                     /locus_tag="VF097_07145"
                     /inference="COORDINATES: protein motif:HMM:NF015799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEX6262604.1"
                     /translation="MEPPKQASLRILIADGDVAHLEAIADLVTALGHEVVAKETSLEE
                     LGAHTAAQRPDVALVLVEEGPTALDRIDEIVQEAVCPVIAVLRGEDPAFINQAARRGI
                     SAYVAADDPEEMQSSIDIVLRRFGEYRGLQDAFERRAVTERAKGILMERHGVDEHTAF
                     LMLRDEARSTRRKLVELAEAVVSGHRLLTADRRPDRTPSGPEEPGD"
     gene            7113..7328
                     /locus_tag="VF097_07150"
     CDS             7113..7328
                     /locus_tag="VF097_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262605.1"
                     /translation="MDELRDEDIRTEDQEPGGAPAPERDADEQDADGVDQADGQDQGD
                     EVDDADQGDQGDRDDDASDEDADQEDA"
     gene            7454..8515
                     /locus_tag="VF097_07155"
     CDS             7454..8515
                     /locus_tag="VF097_07155"
                     /inference="COORDINATES: protein motif:HMM:NF019619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6262606.1"
                     /translation="MDRLVTGGLMRLPGMRLVRDGASVPVGDYTRTIRIGSRRVPETV
                     RPERVVHHFARGATIVLQALHRQWPSIAAFCRELELDLTHPVQANAYVTPATSRGFAV
                     HHDTHDVFVIQTHGRKRWRVYPPVIELAGKEQPWASELGDPGPPILETELSPGDVLYI
                     PRGFPHDATAREEVSIHVTVGIQARTWLDVWRHVMERAPEHGPFREALPIGFARDQEA
                     LAGEIAARLPELRAWLEKAAGPEAAASFAEGFWEGRPPVLGGQLQQVAGLDAVDVTTA
                     LRVRVGAIFRPGREGGEAVARLGNRVLRMPGWVEPALRFIAEARGPFAPRDLAGLDPG
                     SALVLCRRLIVEGVLEADG"
     gene            8508..9389
                     /locus_tag="VF097_07160"
     CDS             8508..9389
                     /locus_tag="VF097_07160"
                     /inference="COORDINATES: protein motif:HMM:NF018677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HEX6262607.1"
                     /translation="MADAPTCSALALAMDEPLQATASRIRHWLLVEQPGRWGHHALLD
                     SELPREVGEHLAIEGHRHGVRVLLIKRRSRRPGGPRRCFAAFTGRRERRLITFQIDAP
                     QDLLDLDLGALARDRWSGLGEEIGGPLFAVCTHGKHDQCCARLGAPLLRALSHRDDTW
                     EVTHVGGDRFAGNLLAFPHGLYFGRVTAEAAGRVVEAYEAGRIALDHFRGRSAFTPAA
                     QAAEHHLRAALGVDGVDDLVLTSHRPLGPLHRVDFMVRGEARVVEVEEVLLETRPLTC
                     KSGVARAPRSFVLRELS"
     gene            complement(9391..9879)
                     /locus_tag="VF097_07165"
     CDS             complement(9391..9879)
                     /locus_tag="VF097_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX6262608.1"
                     /translation="MADETVLVVVEIPRGSRNKYEVDHETGHLVLDRMLFTSMVYPAD
                     YGFIEGTLGSDGDPLDALVFVGEPTFPGCRIRVRPVGLFRMWDEKGADEKILCVPLSD
                     PMWSHVQDLEDVREPLRNEIEHFFAVYKDLEDKPSGVEGFEDRDAAVRVIAEARERTR
                     GA"
     gene            9947..10684
                     /locus_tag="VF097_07170"
     CDS             9947..10684
                     /locus_tag="VF097_07170"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX6262609.1"
                     /translation="MPTRAEIGLGRALRKARHRQGLTLEEASRGTRLRAEYLDALERE
                     SFDDLPGDVYVRSFLRSYSGFLGLKPTKVVGVYEQAVGSGPAAPAPVDRSPAVVVGPN
                     DPLPDVRHHFPWPMAAAVAVIVLVAAAAIGLLSRSNSSPTPALPTAPANLPVLPRHVE
                     VNMVALRDVEARIVVDGVPAFEGIFREDEARSFRGDEEIEVWLATGKLVRLEVNGENL
                     GKPGQPVREFLRTFDRDDFRGDGSGRG"
BASE COUNT         1566 a         3730 c         3930 g         1536 t
ORIGIN      
        1 ggtcgtgcga cgatgggatc gccccaccgc accccgccgg aggggcggac gccgccgcag
       61 cgggaaggag gcagcagatg gccggcagct tcggaaccct cgtcaccgcc atggtgaccc
      121 cgttcaagga cgacggatcg ctcgacctcg accgggcccg cgacctcgcg gcctggctct
      181 tcgatcacgg cacgaacgcg ctcgtcgtca cggggtccac gggcgaggcg gcgacgctct
      241 ccgacgggga gaaggtggag ctctggcggg cgtgcgccga ggtcggcggc ggcatgatca
      301 tcgccgggac cggcacctac gacaccgcgc actccgtcca cctgacccat caggccgacg
      361 aagctggcgt ggacgcgatg ctcgtcgtca ctccctacta caacaagcct cctcagcgcg
      421 gcctgatcga gcacttcacg accgtggccg gggccacctc gaagcccgtc atggtctaca
      481 acatccccgg ccgaaccggc acgcgcatcg aacacgacac gctgctgcgg ctggccgaag
      541 tggagaacat cgtcgcggtg aaggactcga ccggcgatct cgacggcgtg tcccatctcg
      601 tcgctcaggc cccgacggac ttcgagctct actgcggcga cgactgggcc accttcgcca
      661 tgatgtgcgc cggtgcccgc gggctcgtct cggtcgccgc gcacgtcgtg ggggagcgtc
      721 tgaagcggat gatggagctg atcgagggtg gggacgcggc cggagcgcgc aagatccacg
      781 aggagctgtt gcccgttttc gacgcgctgt tcatcacgag caacccgatc ccgctgaagg
      841 cggcgatgga gatgatcggc cagcccgtcg ggccgccgcg cctgccgctc gtcccggcca
      901 ccgacgagga gcgctcccgg atcggcaagg cgctggagga tgcaggcgtc ctctagcctc
      961 gtcttcctcg gcggcgtggg ggaggtgggc cgcaatatgg cctgcctcga gtcgaacggc
     1021 cgcgtcctcg tcctcgacgc gggcctctcg ttccccaccg aggacatgcc cgggatcgac
     1081 ctcgtcctcc ccgactggga gtggctgcgc cggcggcggg accggatcga cggcctgatc
     1141 ctcacccacg gccacgagga ccacatggga gcggtgccct acatccttcg ggagatcccg
     1201 ctgcggatcc acgccacggc cctcgcgctc gcgctcctgc gcccaaagct ggaggagcac
     1261 ggggtcgagg accgggccga gctcgtcgag gtgaacccgg gtcaggagac ccggatcggg
     1321 agcttcaccg cgcggttcca ccgcgtcacg cactcgatcc ccgacggcgt ggcagtggcg
     1381 atcgacatcc ctcaggggac gatcctccat tcgggagact tcaagctcga cccgacgccg
     1441 atcgacgaac ggccgaccga cctgcagggc atcgcggacg aggcgcggcg aggcgtccac
     1501 gtcttcctct cggattccac gaacgccgag gagaagggga cgatccccag cgagcgaacg
     1561 gtcggccccc ggctgctgga gatcttccgg cggggcgagc ggctgatcgt ggcggcctgc
     1621 ttcgccagcc acatccacag ggtccagcag atctgcgacg cggcgcggga ggtggggcgc
     1681 aagatcgcct tcctcggacg ctcgatgcac cagtccgtgg agcaggggcg ccgcctcggc
     1741 tacctcgacg ttcccgagga ggacgtgatc gacatcgagc ggctcaacga ctacgatccg
     1801 cgcgaggtgg ccgtggtctg caccgggagc cagggcgaac cgctgtcggc gctcgccctg
     1861 atggcagccc gcgagcacaa atgggtggac ctcgaggagg gcgacctcgt ggtgatcgcc
     1921 gcgtcgttca tccctgggaa cgagaccgcg atccaccgca cgatcgacgg gctctatcgg
     1981 acgggggccg acgtcttcca ctccaagatc gcggacgtcc acgtgtcggg ccacgcggcc
     2041 gcggacgagc tgcagttcat gctctcgctc gtccgccccc gctggttcat cccgatccac
     2101 ggggagcggc gccatctggc ccaccacgct cgcctggctc ggggggtggg cgtcccctcc
     2161 gaccgcatcc tcatcgtgga ggacggcgac gtcgtggagg tgggagccga ggcggtgaag
     2221 atcgtcgacc gcgccccctc ggggatgatc ttcgtggatg gcctggggat cggcgacgtg
     2281 ggcgacgtgg tcctgcgcga ccgccggaag ctggccgggg acggcttcgt cgtcgtggtc
     2341 gtcaccgccg acgcgcacag cggcgaggtc gtggccgggc ccgacgtcat cacccgcggc
     2401 ttcgtccatg acgagacctc cgtcgagatg ctcgaggagg cgaaggcccg gaccctggcc
     2461 tcgctggccg aggtggccga ggaggaggtg gccgatccga gcatcctccg ccagcacatc
     2521 cggcgcaccc tcggccggta cttcttcgaa gcgattcgcc ggaaaccggt catcgttccc
     2581 gtcatcattg aggtctgatg gcgaggaagc ggaagcggag gacgggcggg gcggtctcgg
     2641 ccgccggcca gaaggtcgcg gagcacctcg ggccgcagct gaccgacgcg ctcggcatcg
     2701 ggctgctggt cctggccggg ctctcggtgc tggggatctg gctcgacgcg gcgggccccc
     2761 tggggcgctt cctgacgatc gtggtggtcg gcatgcttgg gctggccggg tacgccttcc
     2821 ccgtgctcgc cctctactgg gggcttctgc tcgtccgggg cacggccgag gaggaccggg
     2881 gccgcatgtt cgtcggcctg atcttcctgc tgatgggcgg cctgggcatc gtctcgctcg
     2941 ttcgggggaa ccccagcccg gcggcgggct tctcggggtt cggcgatgcc ggcggcctgg
     3001 tcggggcggt gacggcgtgg ccgctctcga aggtgatgtc agtctacggg gcggcgatcg
     3061 tctgcatcgg gctcgtggcg ctcggggccc tcgtcttcac cgccacgccg ctgcgctcgg
     3121 tgtgggagtg gctggtccgg cgcgaggacg aggtcctgga cctcaccgag gtcgaggagg
     3181 cgacgcccaa gcgccgacgg cggaagcagc cggaggccga gggcgagatc ctgacgccgg
     3241 ccctccccga tccggagccc gaggaagagt cgagcccatc cgtcgtcccg agcccctcgg
     3301 gccgcggcta caagcttccc ccggtcgaca tgctgcggaa ggcgccggcg tcgggcgggc
     3361 cggggcggga cgagaaggag acgatggcca tcctggagcg cacgctccgc aacttcggcg
     3421 tcgcggcgag cgtggtgggc gcgagccggg gacccacggt cacgctcttc gaggtcgagg
     3481 tctccgcggg caccaaggtg aacaaggttc tcaacctcgg ggacgacatc gcctatgcgc
     3541 ttgccacgcc ggacgtccgg atcatcgccc cgatccccgg gaggtccgcg atcggcgtgg
     3601 aggtgcccaa caaggtccgc gacttcgtga cgctgggcga cgtgctcggg tcgaaggccg
     3661 cggggaagga gaagcacccg ctggctgtgg ccctggggaa ggacgtccaa gggaaccccc
     3721 tcctgctcag gctgaacgag atgccccacc tcctgatcgc cggggccacc ggcgccggga
     3781 agtcgtcgct gatcaacgcg ttcgtgacct cgatcctgat ccgcgcgcac cccgacgagg
     3841 tgaagctgat gctcgtggac cccaagcggg tcgagctcag ccacttcgcc gatctgcccc
     3901 acctcatctc gcccgtgatc gtggcgccga agcgcgctgc ggaggcgctg gggtgggtcg
     3961 tccgcgagat ggagctccgg tacgagtccc tggccaccgt ggggatgcgg gacatcgatt
     4021 cgtacaacgg ggcacacgcc gagggctcgc tgcgcatccc cccgggccgt gacgcggagt
     4081 tcgagcgcct cccctacatc ctcgtcgtga tcgacgagct ggcggacctc atgatggtgg
     4141 ccccgcgcga cgtggaggac gccatcgtgc ggatctcgca gatggcgcga gccgtcggga
     4201 tccatctcgt cgtggccacc cagcggccca gcgtggacgt ggtgacgggg ctgatcaagg
     4261 cgaacatccc ttcgcggatc gcgttcatga cggccagcgc ggcggactcg cgggtgatcc
     4321 tcgacgtggg cggcgcggag aagctcgtcg gccacggcga cctgctgttc cttccctcgt
     4381 cggcctcgaa ggccgctcgg atccagggcg cgtgggtgac ccagcacgag atcgacgcgg
     4441 tcgccgaata cgtgcggcag cagcgtgagc cggactacgt cgaggggatc gaagggctgg
     4501 ggaagcctcc cgccgacgac gaggacctcg acgcggagga cgatcttctc cagcaggcga
     4561 tggagctcgt gatccattcc cagctcggct cgacctcgat gctccagcgg aagctgaagg
     4621 tggggttcgc gcgcgcgggt cgcctcatgg acatgctcga ggagcggggc gtggtggggc
     4681 cctcggtggg ttcgaagccc cgggacgtat tgatcacttg ggacgagtac gcggagtcca
     4741 gggccggctg aaggacgggc gtcggctcag accgccccct cttcctcgcg ctcgagctcg
     4801 aggttcgagg gcccgccgag caccccgcag atcgtcagca cctccagcac gcgaggtgtg
     4861 ggggcccgca ggacgagtcg accccgatcc gcgtgggacc gtgcgaggcg caggatcgct
     4921 cgaagccccg tggagtcgat gaacgtcagg tccgtcagct cgaggacgag aggatcgtaa
     4981 ggatcgtcgg cgagcccgtc caggctctgc tcgagctcgg gcacggtgga gatgtcgagc
     5041 tcgcctgcca gccggatgcg gtggccggcc tcgacgcggg cctcgaggcg agtcgtcatg
     5101 gttcgctccg atcgagctcg atgcgcaggc gtccctccca gcccagggtc gccgcggcaa
     5161 caaggacccg cagcacgcac gggcgcgggt ggcgcaccgt gatggcgtcg tcgcccttca
     5221 gctccctcgc cgcctcggtc agcacgtgga tgcccgcact gtccatgaac cgcagccccg
     5281 cgagatcgag gaccacctcg cctggactgc tcacgacgtc cgcgatcgcc gaggcgacct
     5341 ccgagcgagc gctcgtgtcg agctcgccga tcagcgcgag cccccgccgc ccgagggcct
     5401 cgatcgagag tctccctgga tcaggggagc gccggacccc gcatcggatg gcggcggcga
     5461 tcgaggaagg ggaccgcccc cgccggccgg ccgaggatcc tgggcgcatc gagcgatggc
     5521 gaggggccgt catcgatcga cccccgtgcg atgcgacctc cgcggattgg tggctctcgc
     5581 gatcctggcc gaccgaccga ccatctcgac gacggcgaag ccgggcgcct cgcgcaccac
     5641 gtagtcgatc tccgggttct cgtgatcgag ggcgatgacg aacctcgcgg ggtaggcgcg
     5701 cacgcgttcg tacttcaggc gatggatcgt gatcggatcg gtgcacgagg tatcgccgca
     5761 ttcgcagagg taggctgcga tcctgctgcc gaggtgctcg gtgtcgtcgt gggtccactc
     5821 gttcatcctc cggaagaccg tcccgttgga gacctcgacg tcgcgccagc tcgacaccat
     5881 cagccaccct ccgccgacga gaagcgcctc agcagctcga acccggccgc tccgtcgagc
     5941 gcggaccccg tgaggtccac gagtcgccgc acggccgggg tgacgccgat cacggcggtg
     6001 cgggcgccct cgcttctcaa gtgaaggagc gcatcgaaca tggcccggag acccatgcga
     6061 tcggcgaagg tcacgtcgcg agcgtcgaag aggacgcctt ccgcgccgtc cgatgagatg
     6121 ctctccagct cttctgccag gcgccgggcg ccggcgaggt ccagggcgcc gcccagcagc
     6181 acgcgcacga ccccattgcg acgctggata cgaaccccga gaggctctgc ggtgggagat
     6241 gcatcgggca cgagaccgcc tcgctcggtt ggtcgacgga aatcgccgac gctgcggcgg
     6301 ccgagggtta gggccctgtc gtgccgagcc gttggctggg gaccggcacc ccccacgcta
     6361 cgactcggag ggggaaccgt caaggggtgt gcccggtcag cccgctcggg tagggtggcc
     6421 gcatggagcc accaaagcag gcgtcgctgc ggatcctgat cgcagacggc gacgtggcgc
     6481 acctcgaggc gatcgccgac ctcgtgaccg ccctgggcca cgaggtcgtg gctaaggaga
     6541 cctccctcga ggagctcggg gctcacacgg cggctcagcg gcccgacgtg gcgctcgtcc
     6601 tggtggagga ggggcccacg gccctggatc ggatcgacga gatcgtccag gaggccgtct
     6661 gcccggtcat cgcggtgctc cggggcgagg accccgcgtt catcaaccag gcggctcgtc
     6721 gggggatctc ggcctacgtg gccgccgacg atcccgagga gatgcagagc tcgatcgaca
     6781 tcgtgcttcg acgcttcgga gagtaccggg ggctccagga cgcgttcgag cgacgcgcgg
     6841 tcacggagcg ggccaagggc atcctgatgg agcgccatgg ggtggacgag cacacggcct
     6901 tcctcatgct gcgcgatgag gcgcgcagca cgaggcgcaa gctcgtcgag ctggccgagg
     6961 cggttgtgtc gggccaccgg ctgctcacgg cggatcggcg gccggaccgg acgccctccg
     7021 ggccggagga gcccggggac tgacgcggcc ggcgtttgca cctcccgagg gccgggcata
     7081 gcctgccgga gagcaagcgg aaggagggct gcatggacga gctcagggac gaggacatcc
     7141 gcaccgagga tcaggagccg ggaggcgccc cggcgcccga gcgcgacgcc gacgagcagg
     7201 acgccgacgg cgtggaccag gccgacggac aggaccaggg agatgaggtc gacgacgccg
     7261 accaggggga ccagggcgac cgggacgacg acgcgtccga cgaggacgcc gaccaggagg
     7321 acgcctgacc gagatcggag cgctcggccg gctcgtcggt gacgaggagc gcttcgcgga
     7381 ggatcactgg gggcgccgcc cgttcctgag ggagggtgcg ggcgacttcg cctcgctcct
     7441 ggatcttccc ggggtggacc ggctcgtcac cggcggcctc atgcgcctcc ccgggatgcg
     7501 gctcgtccgc gacggagcgt cggtccccgt cggcgactac acgaggacga tccggatcgg
     7561 gtcccggcgg gtccccgaga cggtccgtcc ggagcgggtc gtccaccact tcgcgagggg
     7621 agccacgatc gtcctgcagg ccctccaccg gcagtggccg tccatcgccg cgttctgccg
     7681 cgagctcgag ctcgatctca cgcatccggt ccaggcgaac gcgtacgtga cgccggccac
     7741 ttcgcgaggg ttcgccgtcc atcacgacac gcacgacgtc ttcgtgatcc agacgcacgg
     7801 tcggaagcga tggagggtct acccgccggt gatcgagctg gcggggaagg agcagccctg
     7861 ggcgagcgag ctcggggacc ccgggccgcc gatcctggag accgagctgt cgcccggaga
     7921 cgtgctctac atcccgcggg gcttcccgca cgacgccacc gccagggagg aggtctctat
     7981 ccacgtcacg gtggggatcc aggccaggac ctggctcgac gtctggcggc acgtcatgga
     8041 gcgagccccc gagcacggtc ccttccggga ggcgctgccg atcggcttcg cccgagacca
     8101 ggaggccctg gccggcgaga tcgcggcccg actgcccgag ctccgggcgt ggctcgagaa
     8161 ggccgcgggt ccggaggcag ccgcgtcctt cgcggaaggc ttctgggagg ggaggccccc
     8221 ggtcctgggg ggccagctcc agcaggtggc ggggctggac gccgtggacg tgacgacggc
     8281 gctccgcgtg cgggtggggg cgatcttccg gcccggcagg gagggcgggg aggccgtggc
     8341 ccggctcggg aaccgggtcc tgcggatgcc cggctgggtg gagcccgccc tccggttcat
     8401 cgccgaggcc aggggcccgt tcgcccctcg ggacctcgcc ggcctggatc ccggctcggc
     8461 cctggtgctc tgccgccggc tgatcgtgga gggtgtcctg gaggccgatg gctgacgcgc
     8521 cgacctgctc ggcgctggcg ctcgccatgg atgagcccct ccaggcgacc gcctcgcgca
     8581 tccggcactg gctgctcgtg gagcagcccg ggcgctgggg ccaccacgcc ctcctcgaca
     8641 gcgagctccc gcgcgaggtg ggggagcatc tggcgatcga ggggcaccgt cacggcgtgc
     8701 gggtgctgct gatcaagcgg cgctcgcggc gccccggcgg cccccggcgg tgcttcgccg
     8761 ccttcacggg gcgccgcgag cgccgcttga tcacgttcca gatcgacgct ccgcaggacc
     8821 tcctcgacct cgacctcggg gcgctggctc gcgatcgctg gagcgggctg ggcgaggaga
     8881 tcggtggacc gctcttcgcc gtctgcacgc acgggaagca cgaccagtgc tgcgcgcggc
     8941 tgggcgcgcc gctcctccgg gccctgagcc accgagacga cacgtgggag gtcacgcacg
     9001 tggggggcga ccggttcgcc ggcaacctcc tggcgttccc ccacgggctc tacttcggcc
     9061 gggtgacggc ggaggcggcc gggcgagtgg tcgaggccta cgaggcggga cggatcgccc
     9121 tcgaccactt ccggggccgg tcggccttca cccccgccgc ccaggccgcc gagcaccacc
     9181 tccgcgcggc gctgggggtg gacggggtgg acgacctcgt cctgacgagc caccgaccgc
     9241 ttgggcctct ccaccgcgtg gacttcatgg tgcggggaga ggccagggtg gtggaggtgg
     9301 aggaggtcct cctcgagacg cggcccctca cctgcaagtc gggcgtcgcg cgggcgccac
     9361 ggtccttcgt gctccgagag ctctcctgag ttaggcccct cgagtccgct cccgagcctc
     9421 ggcgatgacc cgcacggccg cgtcgcggtc ctcgaacccc tcgacgcccg agggcttgtc
     9481 ctcgagatcc ttgtacacgg cgaagaagtg ctcgatctcg ttccgaagcg gctcccgaac
     9541 gtcctccaga tcctgcacgt gcgaccacat cgggtcgctc agcggcacgc agaggatctt
     9601 ctcgtcggcg cccttctcgt cccacatgcg gaagagcccc acgggccgca cgcggatgcg
     9661 gcagccgggg aaggtgggct cccccacgaa cacgagcgcg tcgagcggat ccccgtccga
     9721 gccgagggtc ccctcgatga agccgtagtc ggccgggtag accatcgagg tgaagagcat
     9781 gcggtcgagg accaggtggc cggtctcgtg atcgacctcg tacttgttcc tcgatccccg
     9841 ggggatctcg accaccacga gcacggtctc gtcggccacg ccgcgagtct agccggggat
     9901 ggccctcgcc gcgggccgac ccctatctgc cctacaatca gtgcccgtgc cgacgcgcgc
     9961 cgagatcggc ctcggtcggg cgctgcgcaa ggcgcggcac cgccaggggc tcacgctgga
    10021 ggaggccagc cgcggcacgc gcctgcgcgc cgagtacctg gatgcgctgg agcgagagtc
    10081 cttcgacgac ctccccggcg acgtctacgt ccgcagcttc ctccgctcgt actcggggtt
    10141 cctggggctg aagccgacga aggtggtggg tgtctacgag caggcggtcg ggagcggtcc
    10201 cgccgctccc gcccccgtcg accggtcgcc cgcggtggtc gtcggcccga acgaccccct
    10261 cccggacgtc cgccaccact tcccctggcc catggccgcg gcggtggcgg tgatcgtgct
    10321 cgtggcggcg gcggcgatcg gcctcctgtc ccgatcgaac tcctccccca cccccgcgct
    10381 cccgaccgcc ccggcgaacc tcccggtcct gccgcggcac gtggaggtga acatggtcgc
    10441 cctcagggac gtggaggcga ggatcgtcgt agacggcgtg ccggcgttcg aggggatctt
    10501 ccgggaggac gaggcccgct ccttccgggg cgacgaggag atcgaggtgt ggctggccac
    10561 gggcaagctc gtgaggctcg aggtgaacgg cgagaacctg gggaagcccg gacagcccgt
    10621 tcgggagttc ctcaggacgt tcgatcgaga cgacttccgg ggggacggat cggggagagg
    10681 ataggcccgc tcgggctcgg ctgggcgaac ctcgtcacgg tcctgcggat cctcctgatc
    10741 cccgtcgtcg tcctgctgat cc
//
LOCUS       DASUTZ010000164         2617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1603753, whole genome shotgun sequence.
ACCESSION   DASUTZ010000164 DASUTZ010000000
VERSION     DASUTZ010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1603753"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VF097_07175"
     CDS             <1..400
                     /locus_tag="VF097_07175"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262610.1"
                     /translation="VLGDVAERLIEGVGPTTVLQTFFGDVAEHGPAFFELPFDAFGLD
                     LVAGPGNLEAIHEFPADRILQAGIVDARNTRLEDSDDLARRIEGLAATVTPDRLWVSP
                     SAGLEYLPREAAQRKCALLAEAARRFRGGR"
     gene            397..1374
                     /locus_tag="VF097_07180"
     CDS             397..1374
                     /locus_tag="VF097_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013987812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX6262611.1"
                     /translation="MSGVLLTTQVGSLPKPDYLLRARSAFARDEISREELRDLERRAT
                     REWVEFQNEIGIDVVVDGEQYRGDMVAFFAEELEGFSRGGLVRSYGNRYYRKPIITGP
                     IRRTHPITVEWWSYASSLTDSPVKGMLTGPYTIRDWSFDEHYDTRRDAVLALAEAIRD
                     EAVALQEAGAEYVQIDEPAVSTRMDELDLAIEALGVVTEPLSIHTITHICYGDFHNAY
                     PRMLDLPVDQIDLELTNSRFDLLDRFAEHPFTKSIGLGVVDVHSHRIETVDDVVKGIE
                     RTLEVIPPERIFVDPDCGLKTRSVEEAKDKLRVVVEGARRVRSRLGRYS"
     gene            1442..>2617
                     /locus_tag="VF097_07185"
     CDS             1442..>2617
                     /locus_tag="VF097_07185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELWxxDGT repeat protein"
                     /protein_id="HEX6262612.1"
                     /translation="MPRHTIRTLVVIIALVGPSLLPGIAGAAPRVRLVKDIHPGGDSN
                     PGGFVSLGNRTLFSADDGVLGPELWRTDGTRRRTKRLKDIWPGAGGSAPRSLTRMGKR
                     ILFVADDGLHGLELWRTNGRPSGTRLVKDINPGGSAFPAMGPIDLVGVGGTLFFAADD
                     GTRGRELWKSDGTRRGTKLVKNIWPGADDGSPGELTRFGKRLLFVADDGTRGRELWRS
                     DGTRRGTKLVKDVRPAGDSSLPNFITAFRKVAYFQADDGTRGRELWRTNGRPKGTRMV
                     KDIRPGPDGSSPEDFFMVGGRLIFEANDGTRGFEPWRSDGTRKGTRILKDVAPGMTSS
                     SPDYGFVFRGILYQTMSDGTRGSELWRTDGTRRGTRLVKDIWQGVDGSIPRYFARAGR
                     "
BASE COUNT          413 a          875 c          950 g          379 t
ORIGIN      
        1 cgtgctcggc gacgtcgccg agcgcctgat cgagggcgtg ggcccgacga ccgtgctcca
       61 gacgttcttc ggcgacgtcg ccgagcacgg ccccgccttc ttcgagctcc ccttcgacgc
      121 gttcggcctc gatctcgtgg ccggtccagg gaacctcgag gcgatccacg agttcccggc
      181 ggaccggatc ctccaggcgg gcatcgtgga cgctcgcaac acgcggctgg aggactcgga
      241 cgacctggcc cggcggatcg agggcctggc cgcgacggtc acccccgatc ggctctgggt
      301 gagcccctcc gcgggcctgg agtacctgcc gcgcgaggcc gcgcagcgga agtgcgcgct
      361 gctggccgag gcggcccgac ggttcagggg gggccgatga gcggcgtgct gctgacgacc
      421 caggttggga gccttcccaa gcccgactat ctcctccggg cccgctcggc gttcgcacgg
      481 gatgagatct cccgggagga gctgcgggat ctcgagcgcc gcgccacccg cgagtgggtg
      541 gagttccaga acgagatcgg gatcgacgtc gtcgtggacg gcgaacagta ccgcggcgac
      601 atggtggcgt tcttcgccga ggagctcgag gggttctctc gcggcgggct cgtccgctcc
      661 tacggcaacc gctactaccg caagccgatc atcacgggac cgatccggcg gacgcacccc
      721 atcaccgtgg agtggtggtc gtacgcgagc tcgctcaccg acagcccggt gaaggggatg
      781 ctgactgggc cctacacgat ccgggactgg tccttcgacg agcactacga cacgaggcgc
      841 gacgcggtgc tggcgctggc cgaggcgatc agggacgagg ccgtggcgct gcaggaggcc
      901 ggggccgagt acgtccagat cgacgagccg gcggtctcca cgcggatgga cgagctcgac
      961 ctggccatcg aggcgctcgg cgtggtgacc gagccgctct cgatccacac gatcacccac
     1021 atctgctacg gcgacttcca caacgcctac ccgcggatgc tcgacctgcc cgtggaccag
     1081 atcgacctgg agctgaccaa ctccaggttc gacctcctgg accgcttcgc cgagcacccg
     1141 ttcacgaagt cgatcgggct cggcgtggtg gacgtccact cgcaccggat cgagacggtg
     1201 gacgacgtgg tgaaggggat cgagcggacc ctcgaggtga tcccgcccga gcggatcttc
     1261 gtcgatccgg actgtgggct gaagacccgc tcagtcgagg aggccaagga caagctccgc
     1321 gtcgtcgtcg agggcgcccg ccgggtccgg agcaggctcg gccgctactc ctagccttcc
     1381 gtggcccgat gccgtacgat gcggccctcc ggtcggcgtt ccaagcgaga gggaggctca
     1441 cgtgcccagg cacaccatcc gcaccttggt cgtcattatt gctctcgtcg ggccctcgct
     1501 cctccccggc atcgcaggcg ccgctccgcg cgtccggctc gtgaaggaca tccatccggg
     1561 aggcgactcc aaccccggcg gcttcgtttc cctggggaat cgcacgctct tctcggcgga
     1621 cgacggcgtc ctcggccccg agctctggag gaccgacggg acccggaggc gcacgaagcg
     1681 cctgaaggac atctggcccg gcgcgggcgg atcggctcca cggtccctga cccgcatggg
     1741 gaagcggatc ctcttcgtgg cggacgacgg cctccacgga ttggagctct ggaggacgaa
     1801 cgggaggccg tcgggtacgc ggctggtgaa ggacatcaac ccgggggggt ccgccttccc
     1861 cgccatggga ccgatcgatc tggttggggt gggcggaacg ctgttcttcg ccgccgacga
     1921 cgggacgcgg ggccgtgagc tgtggaagtc ggacgggacc cgccggggca cgaagctggt
     1981 caagaacatc tggccgggcg cggacgacgg ctcgccgggg gagctgacgc ggttcgggaa
     2041 gcgcctcctg ttcgtggccg acgacggcac ccgaggccga gagctgtgga ggtcggacgg
     2101 gacccgccgg ggcacgaagc tggtcaagga cgtccggccg gcgggggact cgagcctccc
     2161 gaacttcatc accgccttca ggaaggtcgc ctacttccag gccgacgacg gcacacgagg
     2221 tcgggagctg tggcgcacga acggcaggcc caagggcaca aggatggtca aggacatcag
     2281 gccgggcccg gacggcagct cccccgagga cttcttcatg gtgggaggtc gcctgatctt
     2341 cgaggcgaac gacggtaccc ggggcttcga gccctggcgc tcggacggca cgcggaaggg
     2401 gacgaggatc ctgaaggacg tcgcgccggg gatgacctcg agcagccccg actacggctt
     2461 cgtcttccga gggatcctct accagacgat gtccgacggg acccgtggat cggagctgtg
     2521 gaggacggac gggacccgac gaggcacgag gctggtcaag gacatctggc agggggtcga
     2581 cgggtcgatc cctcggtact tcgccagggc ggggcgc
//
LOCUS       DASUTZ010000165        13842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_437689, whole genome shotgun sequence.
ACCESSION   DASUTZ010000165 DASUTZ010000000
VERSION     DASUTZ010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13842
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437689"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1946)
                     /locus_tag="VF097_07190"
     CDS             complement(<1..1946)
                     /locus_tag="VF097_07190"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6262613.1"
                     /translation="MLRATLKGLLAHKFRLALTAIAVVLGVAFVAGTLVLTDTIAKAF
                     DEVFANAQEGVDVEVRAQASDLAAGGDRERIPEELLSVIQRVEGVDRVEGGVTGYAQI
                     VDESGEAIGTTGAPTFGISWPTYEGFSALSIREGRAPLTGGEVAIDAATAERHGFAIG
                     DRVTVLFQGPQEEFQVVGIAGFGDADNLAGATMAAFDVATAQRVLGAEDTFDSIFVAA
                     DEGVGAGELRDRISEALPQGVEAQTGAAVAAEQANAIKDALGFFNTFLLVFAAIALFV
                     GAFIIFNTFNILVTQRTRELALLRALGASPRQVTLSVLTEAGIVGLAASVVGLGLGIL
                     VAFGLRALLAGFGIDLPSASLQILPRTIAVAFLVGILVTLVAALMPARRAARISPIAA
                     MKAVEAVRPLDSRRRMVIGGALVAGGVALGLVGLFGGGDSALQAVGIGVAATFIGTAI
                     LSPLIAQPLARAIGSPLPRLAHLPGKLGRENAMRNPRRTASTSAALMIGLALVGTFLI
                     LGSSLKTSVGQTIERTLRADYVIQPGVGTMAGFSPELAGLLADSPDVTALSPLRFGQW
                     REEGSGATSWLSGVDPAQIEQVLFLDVAEGDLASLDDDGVFVREDEAEAKGLEVGETL
                     RMEFGAAGVVPMEVRGIYREGWLAG"
     gene            complement(1950..2702)
                     /locus_tag="VF097_07195"
     CDS             complement(1950..2702)
                     /locus_tag="VF097_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003936171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6262614.1"
                     /translation="MSIGQDVTANGTAARAVEAVKVYGSGEAEVRALDGITAGFARGT
                     FTAIMGPSGSGKSTLLHCLAGLDTLTTGRAFIGDADLTTLPERELTRIRRDKIGFIFQ
                     AYNLIPTLSAGENITLPLDIASRKHEEDWLASVVETMGLGDRLHHRPSELSGGQQQRV
                     AAARALVARPDIVFADEPTGNLDSKSGAELLAFLEGVVRDFGQTIVMVTHDPKAAAHA
                     DRVVFLADGRIADEMQGPTAESVLDRMKSLEA"
     gene            2834..4036
                     /locus_tag="VF097_07200"
     CDS             2834..4036
                     /locus_tag="VF097_07200"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX6262615.1"
                     /translation="MADTTDERQDVYTAMGEQWLLRGGGVDLIRAEGQDLSTEEMVLN
                     IGPQHPSTHGVLRIVLELDGERIRRAEPVIGYMHRAAEKLAEHRDWRQVLVLMNRHDW
                     LSAFNNELGWTIAVERMLGVEVPERAQWIRTMMAEWNRVLNHLMFMGSYPLELGAMTP
                     MFYAFREREMIQDLMESATGARMHHSYARVGGLKDDLPRGFLKRSGEVLNWIRAKLRD
                     FDNLIMGNEIIHNRTRGIGVLPPDVAVSYGVSGPVLQASGVPMDSRKDEPYEKYGEVE
                     FDVPVGRNGDCYDRLWVLVQRMHQSCNIVEQCMDRLPAGEYRSTSLPKKINIPEGEIY
                     VRTENPLGLMGYYVVGDGGEYPYRLKMRTASFSNVSVLPWLLRDQLLPDLIAILGSVF
                     FVVGDVDR"
     gene            complement(4205..5299)
                     /locus_tag="VF097_07205"
     CDS             complement(4205..5299)
                     /locus_tag="VF097_07205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX6262616.1"
                     /translation="MSDLEARLLERIRREGPITFADFMEAALYDREGFYAKLPIGAEG
                     DFVTSPHVSPAFGSLIARQLAQCWELLDRPAPFRVVEVGAGDGTLARQVMAEAGSVPP
                     LADALAYAAVERSPAARRAVAEAGIEARERLEGPAHVVLANELLDNLPFHRVRGGPEG
                     PVEVWVGATRRGIEELEGPPSAAALAALEAVGRPLEPGEERPVSPAALAFVDGVAGAL
                     APGYAFLFDYGFTGGERPGDPQAYRRHRVSGDLLADPGSRDITAAVDLDAIAARARAA
                     GLQVWGPVTQREALLGVGMRLWLQGVRRRTQQAEEQGDLDEARRLFEARSRASILIDE
                     TKLGGLRLLTLGSEGLPAPAAALGDSQTGC"
     gene            5352..6005
                     /locus_tag="VF097_07210"
     CDS             5352..6005
                     /locus_tag="VF097_07210"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX6262617.1"
                     /translation="MEPRPAATVVVARPADPGVEVLVLRRSASSRFAPGFLVFPGGVV
                     EERDVALAERLFGRPDEAPRACAVRELYEETSLLLARGGLEPHPDRPPLEGRAFDPPA
                     REDLVEIARWVAPESLVERFDAIFYAIAAPRGLDPLPDHVEADDAWWADPEEVVVEST
                     RGESPLMWPTLITLERLASVSSVEEVLSLRIEQLEPGLEERPARVRGAWRRPEGRRP"
     gene            6002..6874
                     /locus_tag="VF097_07215"
     CDS             6002..6874
                     /locus_tag="VF097_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6262618.1"
                     /translation="MIRIRRVLAPNPGPFTLEGTNTWIVGERPSLVIDPGPDDAGHLL
                     TVADEAEPLEAILLTHRHPDHAPGAGRLSAATGAPVYALRPEGDERPIRHNQVLEMGG
                     LAVRAIHTPGHTSDHLVFLLDSDGAMFTGDTILGRGTSVVDPPDGDMAAYVRSLGELL
                     ALRPRVIYPGHGPVVFDATGRIEAYLSHREQREAQVLEALSEGRSSPEEMVPGIYGGD
                     VPEAMFPHAARSVLAHLLKLEREGRAARTGRLSDNRFVVVREAPCVRCGRPARVGSSL
                     CRRCSLDALQEGPG"
     gene            6892..7272
                     /locus_tag="VF097_07220"
     CDS             6892..7272
                     /locus_tag="VF097_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262619.1"
                     /translation="MRAEFLRPEDPETVVGTVEWDGVRVTLRADDESRATLQRVFRVS
                     SVPIDDPSRRALGTSGPVLAEPGDLDWFLAAARVRGRQEGLTVRLVPGTAGGWDPAGA
                     YRRMGDWIGEREASARETHPASGR"
     gene            complement(7274..7504)
                     /locus_tag="VF097_07225"
     CDS             complement(7274..7504)
                     /locus_tag="VF097_07225"
                     /inference="COORDINATES: protein motif:HMM:NF024776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6262620.1"
                     /translation="MDSGRLLRRAQNKELPFSALPAIRELRGYLDSLETKIVLLARDK
                     GATWDEIAEAMGITRQALYQRMRNRERRGSRG"
     gene            7596..10484
                     /locus_tag="VF097_07230"
     CDS             7596..10484
                     /locus_tag="VF097_07230"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX6262621.1"
                     /translation="MSWEEAQTRVLDHAGGGMVVIGPPGSGKSSLLRERFVRLVDAGV
                     DPERVVLFVLDRRAARRARDDLIHRLRRSLPDLPVFTVHGFAFRVLGAAFERLGYPEP
                     PQVLSAPEQYAFVRGILQEEDPADWPRLGAYLSEPDFAAQAADFVLRAQERLLDPEEL
                     AGMSSRDEDREMASFYRRYLDAIASAGQVDFAGLLFQSAKVLDEEDPGYRHVLVDDYQ
                     DATHAAEGILRSLASRAESVVVAADPGGHVFSYRGGTLEPYRRLDETLGRPERVELPG
                     SRRLSDQGRSLEELERAEASGVEAPPWLSARLYAHPGEEAEAIAHELLQRRVDEDLPW
                     EAMAVVLRRYGGYLTAIRHALARHRIPFVVVAEAAEIAAEPANRPIVDLLRFVFRPER
                     RGELLEPVLTSPIGGLDPHETRRLRREARRAGTPLLHLVLEAALEDLPGPLRGRVLAL
                     RELFDRMPAVEEERGADGLFHWLWTRLPHVRGLVAEEGRQRDLDALAALAGVLSRFVE
                     RRPGSGIEDYLDTLDAAGFGPDPWIPPEERHPQAVRIISAHRAQGLEFDVVLIAGCLE
                     GEFPSLGARSALVDLESIVDPKPPTERLRDRLAEERALFRLAVSRARLATVLFASTSS
                     GARRPRRPSRFAERLGIVWEPPAEVAPPAVSLRSMEAALRLRLADPEAPRHERFAALA
                     ALPAIGARPSAWWHGRDWTDPGRPIREGEFQTSFSRLEALEACGLLYLGRTELGLDPD
                     ATHYMWLGSVIHEIIERVQKGELPRDVDALVEALDAAWEPEQFPHRAVEHRRRLDAEA
                     MLRRWVEHDRREAIASERAFEFEIARARVRGRIDAIFQMNNGGLHILDYKSGKNAPTQ
                     KDIEGNLQLATYYLGVKRDPELSQLGEPKYLELAFLGKDHYKDGFVKRGFMASKVEGY
                     EDLSEGRLEELIRRVRDEDFKPVANPECRLCALRTICAIQIEGGEAPV"
     gene            10481..13750
                     /locus_tag="VF097_07235"
     CDS             10481..13750
                     /locus_tag="VF097_07235"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX6262622.1"
                     /translation="MSAASFHEGTPREIVEALGGRTPTVQQWEAISAGLEPAAVVAGA
                     GSGKTAVMAARVVYLALVHAGRLEADHPGAPPSRVLCLTFTNKAAEELADRVGRATAG
                     LGLPDGEEATVLTYHSFAAALIDEHGMRLGLPATARLLSEGERWQIAERLLDDRELEW
                     IPTAWVPSLVGDTLALADACANQLIDPEELAARELATVEALGEPEGRVDKTRRTIGRQ
                     RSELARMVADFQAAKREMGVVDYGDQIAHAAKLAKEHPEVGEWFRERYSVVLLDEYQD
                     TNLAQADLMRHLVGRGFPIFAVGDPDQNIYAWRGATLRNILGFGDDFGASNGELPLYV
                     NFRSGSRILAAANNVIEPVPPERRAAGKILHRHEPLGEGRVLGFVAEDQRAEAGQIAR
                     MIKEMRGSPRRDGGALQYGDFVVLCRKKRLFGPIAEVLRQENLPVEIVDLGGLLQLPE
                     IVDVVAWLRLLENPGDNVSLARILFGPRWRIGYRDLAILAHWSADQNRSLADELDADR
                     SPGEVRFALVEAVDHLDEIEGLSPEARERLEEFRGDLAGLRERVRQPLGDLVAEVFEV
                     SGLLRELEASTSPPARAARRNLLNLVDHVAAFTSLDGEATLSAFIAYLNAALEAEDEL
                     EPAQASESDTVKLMTVHKAKGLEWPVVFVPGMAEHERPASSIFPNLRTRENPLLGKTA
                     IPLEMRRDEADFPDFEGDIDAWGAALREQQLEEERRLGYVALTRAQETLVVSAAYWYE
                     GPQDPYGPSRFFDEVTGSPGAEVLFVADRPDENPIAALLAEKAADWPGPGRREDADEL
                     FPGGWRAAAIDAVGSPDGAERRVESVLGPRDLEAFRRLVASHRERAARIGERVTSEGP
                     PPLPAALSVSSVIDLERCPKLFYWSHVRPLPRRPSSAARLGSEVHRWIELQSSGQASL
                     LDVDEIPDMGTEERMGDPGIVAEMRRAFRESRFSESVPLYAERPFLLYLEGSVVGGRI
                     DAIFGEPHGPWEVVDYKTGRKPDDDPLAGLQLDLYALACVEIWGKAPEELTLTYFYLA
                     SGEEETRPAGDPEETRKRVLEAIRRAASGEFEATPGPQCRWCDFREFCEPGAIWVREH
                     G"
BASE COUNT         1923 a         5035 c         4934 g         1950 t
ORIGIN      
        1 ccggccagcc agccctcccg gtagatccct cgaacctcca tggggacgac gccggcggcc
       61 ccgaactcca tgcgcagggt ttcgcctacc tcgagcccct tcgcctcagc ctcgtcctct
      121 cggacgaaga cgccgtcgtc atcgagcgac gcgaggtccc cctcggccac atcgaggaag
      181 aggacctgct cgatctgtgc agggtcgacc cccgagagcc aggaggtcgc gccggagccc
      241 tcctctcgcc actgcccgaa gcgcaggggc gagagggccg tcacgtcggg gctgtcggcg
      301 aggaggccgg cgagctcggg actgaagccg gccatcgtgc ccacgcccgg ctggatcacg
      361 tagtcggccc ggagcgtgcg ctcgatcgtc tggcccaccg aggtcttcag cgacgagccc
      421 aggatcagga acgtccccac cagggccagg ccgatcatca gcgccgccga ggtggatgcc
      481 gttcgccggg gattgcgcat cgcgttctcg cggcccagct tgcccgggag gtgggcgagc
      541 cgggggagag gggacccgat cgctcgcgcc agcggctggg cgatgagggg gctcaggatg
      601 gccgtgccga tgaaggtggc ggccacgccg atcccgaccg cctgcagcgc gctgtcgccc
      661 ccgccgaaga ggcccacgag cccgagagcg acccctcccg ccaccagcgc acctccgatg
      721 accatccggc gcctcgagtc gaggggccgc accgcctcca cggccttcat ggcggcgatc
      781 ggcgagatgc gggcggctct gcgcgcgggc atgagcgccg cgacgagcgt gaccaggatt
      841 ccaacgagga acgccaccgc gatcgtcctc gggaggatct gcaggctcgc cgatgggagg
      901 tcgatcccga agccggccag gagggcccgc aggccgaacg ccacgaggat ccccagcccg
      961 aggcccacga ccgacgcggc cagccccacg atgcccgcct cggtgagcac cgagagcgtg
     1021 acctggcgcg ggcttgctcc cagggcccgc agcagcgcca gctcccgcgt ccgctgggtg
     1081 acgaggatgt tgaacgtgtt gaagatgatg aaggcaccca cgaagagcgc gatcgccgcg
     1141 aacaccagga ggaaggtgtt gaagaagccg agcgcatctt tgatcgcgtt ggcctgctcc
     1201 gcggccaccg ccgcccccgt ctgcgcctcg accccctggg ggagagcctc ggagatccgg
     1261 tcccggagct cgcccgctcc caccccctcg tcggccgcca cgaagatcga gtcgaaggtg
     1321 tcctcggccc ccagcacccg ctgcgctgtg gcgacgtcga acgcggccat cgtggccccc
     1381 gcgaggttgt ccgcgtcgcc gaaccccgcg atgcccacca cctggaactc ttcctgcggt
     1441 ccctggaaga gcaccgtgac ccggtcgccg atggcgaagc cgtgacgctc cgcggtggcg
     1501 gcgtcgatcg ccacctcgcc ccccgtgagg ggggcccggc cctcgcggat cgagagcgcc
     1561 gagaagccct cgtaggtcgg ccacgagatc ccgaacgtgg gggcgcccgt ggtgccgatc
     1621 gcctcgccgc tctcatccac gatctgcgcg tagccggtga cgccgccctc gacccggtcc
     1681 accccctcga ccctctggat caccgagagg agctcctccg ggatgcgctc gcggtcgccc
     1741 cccgcggcca ggtcggaggc ctgggccctg acctcgacgt ccacgccctc ctgggcgttg
     1801 gcgaacacct cgtcgaacgc cttggcgatc gtgtcggtga gcacgagggt cccggccacg
     1861 aaggcgaccc caagcacgac ggcgatcgcg gtcagcgcga gcctgaactt gtgggccagc
     1921 agccccttga gcgtcgcccg cagcatggct caggcctcca gcgacttcat gcggtcgagc
     1981 accgactcgg ccgtggggcc ctgcatctcg tcggcgatcc gcccgtccgc caggaagacc
     2041 acccggtcgg cgtgggccgc cgccttgggg tcgtgcgtga ccatcacgat cgtctggccg
     2101 aaatcccgga ccacgccctc caggaaggcc agcagctccg cccccgactt ggagtcgagg
     2161 ttcccggtgg gctcgtcggc gaagacgatg tcgggcctgg ccacgagcgc gcgggctgcg
     2221 gccacgcgct gctgctggcc gccggagagc tccgaggggc ggtggtgcag gcgatcaccc
     2281 agccccatcg tctccacgac gctcgcgagc caatcttcct cgtgcttcct cgaggcgatg
     2341 tccaacggca gggtgatgtt ctccccggcg ctcagcgtgg ggatcaggtt gtaggcctgg
     2401 aagatgaagc cgatcttgtc gcggcggatc cgcgtgagct cccgctccgg gagggtggtc
     2461 aggtccgcgt cgccgatgaa ggctcgcccc gtcgtcagcg tgtccaggcc tgcgaggcag
     2521 tggagcagcg tggacttccc ggagcccgag gggcccatga tcgcggtgaa ggtgccccga
     2581 gcgaaccccg cggtgatgcc gtcgagggcg cggacttccg cctcacccga cccgtacacc
     2641 ttgacggcct ccacggcacg tgcggcggtg ccgttggctg tcacgtcctg cccgatgctc
     2701 atcgaacgtc tccctcctcg tggcacgtct gggggccgtc gaccgtctgc gcgggcgcca
     2761 aggatagacg cttccgctcg tggggtgaag ggttgcggct aggacggccc gttcgagtac
     2821 cctaggcgcg tcgatggccg acacgaccga cgagcgccag gacgtctaca ccgcgatggg
     2881 ggagcagtgg ctcctccgag gcgggggcgt ggacctgatc cgggccgagg gccaggacct
     2941 ctccaccgag gagatggtgc tgaacatcgg cccccagcac ccctcgaccc acggcgtgct
     3001 gcggatcgtg ctcgagctcg acggcgagcg gatccgccgg gcggagccgg tgatcggtta
     3061 catgcaccgg gccgctgaga agctcgcaga gcaccgggac tggcgccagg tgctcgtgct
     3121 gatgaaccgc cacgactggc tctcggcctt caacaacgag ctggggtgga cgatcgccgt
     3181 ggagcggatg ctgggcgtcg aggtccccga gcgcgcccag tggatcagaa cgatgatggc
     3241 cgagtggaac cgggtcctga accacctgat gttcatgggc tcgtacccgc tggagctcgg
     3301 cgcgatgacc ccgatgttct acgcgttccg cgagcgggag atgatccagg acctgatgga
     3361 gtcggccacc ggggcccgca tgcaccactc ctacgcccgg gtggggggcc tgaaggacga
     3421 cctcccccgt gggttcctga agcgctccgg cgaggtgctg aactggatcc gggccaagct
     3481 tcgcgacttc gacaacctca tcatgggcaa cgagatcatc cacaaccgca cgagggggat
     3541 cggggtcctg cccccggacg tggcggtctc ctacggcgtc agcgggccgg tcctgcaggc
     3601 ctctggcgtc ccgatggact cgcgcaagga cgagccctac gagaagtacg gcgaggtcga
     3661 gttcgacgtg ccggtggggc ggaacggcga ctgctacgac cgcctgtggg tgctcgtcca
     3721 gcggatgcac cagtcgtgca acatcgtcga gcagtgcatg gataggcttc ccgcgggcga
     3781 gtaccgatcc accagcctcc ccaagaagat caacatcccc gagggcgaga tctacgtgcg
     3841 caccgagaac ccgctcgggc tgatgggcta ctacgtcgtc ggcgacggtg gtgagtaccc
     3901 ctaccgcctg aagatgcgca cggcctcgtt ctccaacgtg tccgttcttc cgtggctgct
     3961 ccgggatcag ctgctgcccg atctcatcgc gatcctgggc tccgtcttct tcgtggtcgg
     4021 cgacgtggac cggtagtagc cgctcccggc agaggacctg cccgcgctgc ggcgccgtcg
     4081 tcccgtccga gttccgattc tgccgccgcc gcggcaccga tcttcaggcc acggcggact
     4141 cgcttccgca gagcatgcgg gacacgttcc tagagctgcc ctggatcgcg cgggtcgtcg
     4201 gccctcagca gcccgtctga gagtcgccca gggccgcggc cggcgccggc agcccctccg
     4261 agcccagcgt cagcaggcgc aggcccccca gcttcgtctc gtcgatcagg atcgaggcgc
     4321 ggctgcgagc ctcgaacagc cgccgcgcct cgtcgagatc gccctgctcc tccgcctgct
     4381 gcgtccggcg ccgcacgccc tggagccaga ggcgcatccc gacgccgagg agagcttcgc
     4441 gctgggtgac cggcccccac acctggagcc cggcggcgcg ggcccgggcc gcgatcgcgt
     4501 cgaggtcgac cgccgccgtg atgtccctgc tccccgggtc ggccagaagg tccccgctca
     4561 cccggtggcg tcggtaggcc tgggggtcgc cggggcgctc gcccccggtg aacccgtagt
     4621 cgaagaggaa cgcgtacccg ggggcgagcg ccccggcgac gccgtccacg aaggccagcg
     4681 ccgcgggaga gaccggccgc tcctcgcccg gctccagcgg ccgacccacc gcttccaggg
     4741 ccgcgagcgc tgctgccgac ggggggccct ccagctcctc gatccctcgg cgcgtcgcgc
     4801 cgacccagac ctcgaccggc ccctccggcc ctcctcgcac gcggtggaac ggcaggttgt
     4861 ccaggagctc gttggccagc acgacgtggg cgggcccctc gagccgctcc cgagcctcga
     4921 tcccggcctc ggccacggcc cgccgcgccg ccgggctccg ctcgacggcc gcgtacgcca
     4981 gggcgtcggc caggggcggg acgctcccgg cctccgccat cacctggcgg gccagggtcc
     5041 cgtcccccgc tccgacctcc acgacgcgga agggggccgg ccggtccagc agctcccagc
     5101 actgcgcgag ctggcgggcg atcagcgagc cgaacgctgg cgacacgtgg ggactggtca
     5161 cgaagtcgcc ctcggccccg atcgggagct tcgcgtagaa cccctcccgg tcgtagagag
     5221 ccgcctccat gaagtcggcg aacgtgatcg gtccctctcg cctgatccgc tccaggagcc
     5281 gggcctcgag atcgctcacc ggggcgagcc tagcaggcgc tcccgcccgg ccggtctgcc
     5341 agaatcgcgg cgtggagccc aggcctgccg ccaccgtcgt cgtggcgcgc cccgccgatc
     5401 ccggcgtgga ggtgctggtc cttcggcgct cggcctccag ccggttcgcc ccggggttcc
     5461 tggtgttccc ggggggcgtc gtcgaggagc gcgacgtcgc gctcgccgag cgactcttcg
     5521 gccgtccaga tgaggctccc cgcgcctgcg ccgtccggga gctgtacgag gagacgagcc
     5581 tgctcctggc gcggggcggc ctcgagcccc accccgaccg gcctccgctg gagggccggg
     5641 ccttcgaccc tccggcccgc gaggacctcg tcgagatcgc tcgctgggtg gcccccgaga
     5701 gcctcgtcga gcgcttcgac gcgatcttct acgcgatcgc agccccccgg ggcctcgacc
     5761 ccctccccga tcacgtcgag gccgacgatg cctggtgggc ggacccggag gaggtcgtcg
     5821 tggagagcac gcgcggcgag agcccgctga tgtggcccac gctgatcacc ctggagcgcc
     5881 tggcctcggt ctcgagcgtg gaggaggtgc tgtccttgcg gatcgagcag ctcgagccgg
     5941 ggctcgagga gcggcccgcg agggtgcgtg gcgcctggcg gaggcccgag gggaggcgtc
     6001 cgtgatccgg atccggcgcg tgctggcccc gaacccgggc ccattcacgc tggagggcac
     6061 gaacacgtgg atcgtagggg agcgtccgag cctcgtgatc gaccccggcc ccgacgacgc
     6121 cgggcacctg ctcaccgtgg ccgacgaggc cgagcccctc gaggcgatcc tcctcacgca
     6181 ccgccatccg gaccacgcgc ccggggcggg gcgtctctcg gccgcaaccg gggcgcccgt
     6241 ctacgccctg cggcccgagg gggacgagcg accgatccgc cacaaccagg tcctggagat
     6301 gggagggctc gccgtgcggg ccatccacac cccggggcac acctcggacc acctcgtctt
     6361 cctgctggac tccgacgggg cgatgttcac gggcgacacg atcctggggc gagggaccag
     6421 cgtcgtggac ccgcccgacg gcgacatggc ggcgtacgtg cgctcgctcg gcgagctcct
     6481 cgccctccgc ccccgggtga tctacccggg acacggcccg gtggtcttcg acgcgaccgg
     6541 gaggatcgag gcctacctct cccaccggga gcagcgggag gcgcaggtgc tcgaggcgct
     6601 gtccgagggg aggtcctctc ccgaggagat ggtccccggc atctacggcg gcgacgtccc
     6661 ggaggcgatg ttcccgcacg cggcccggtc ggtcctggcg cacctgctca agctggagcg
     6721 cgaggggcgc gcggcgcgga cggggaggct ctcggacaac cgcttcgtgg tcgtccggga
     6781 ggccccgtgc gtccggtgcg gccggccggc gcgcgtgggc tcctcgctct gcaggaggtg
     6841 cagcctcgac gctctgcagg agggaccggg ctgagtgggt atccttcggg tgtgcgcgcc
     6901 gagttcttgc gacccgagga tccggagacc gtcgtcggca cggtcgagtg ggacggcgtc
     6961 cgcgtcaccc tgcgggccga cgacgagtcg cgggcgacgc tgcagcgggt gttccgggtc
     7021 tcatcggtgc cgatcgacga tccatcgcgc cgggccctgg ggacgagcgg cccggtcctg
     7081 gccgagcccg gggacctcga ctggttcctg gccgcggcgc gggtacgtgg gcggcaggag
     7141 ggcctgaccg tccgcctcgt tccgggcacc gcgggcggct gggacccggc cggtgcctac
     7201 cggcggatgg gcgactggat cggggagagg gaagcgagcg cgcgggagac gcatcccgcg
     7261 tccgggcgtt gaatcagccg cgcgatccgc ggcgttcccg gttccgcatc ctctgataga
     7321 gcgcttggcg tgtgatgccc atggcctcag cgatctcgtc ccaggtcgca cccttgtccc
     7381 gggcgaggag aacgatcttc gtctcgagcg aatccaggta gccgcgcagc tctcggatcg
     7441 cggggagggc cgagaagggc agctccttgt tctgtgctcg ccgaagcagg cgtccggagt
     7501 ccatgcccga aaccctagcg aagggcgagg gggagaggca aagcgcggcc gcgatccggg
     7561 ccgacgtgtc agaccctggc ggtagcatgc gattgatgtc ctgggaggaa gcgcagacga
     7621 gggtgctcga tcacgctggg ggcgggatgg tcgtgatcgg gccccccgga tcggggaaga
     7681 gctccttgct ccgggagcgg ttcgtccgtc tcgtcgatgc gggggtcgac cccgagcgcg
     7741 tcgtcctgtt cgtcctggac cgacgcgcgg cccgccgggc acgcgacgac ctgatccatc
     7801 gcctgcgccg ctccctcccg gacctccccg tcttcaccgt ccacggcttc gcgttccgcg
     7861 tgctgggggc cgcgttcgag cgtctcggct accccgagcc cccccaggtc ctctccgcgc
     7921 ccgagcagta cgcgttcgtg cgggggatcc tgcaggagga ggaccccgcc gactggcctc
     7981 gcctcggcgc gtacctctcc gagcccgact tcgccgccca ggccgccgac ttcgtcctcc
     8041 gagcccagga gcgcctgctc gacccggagg agctcgccgg gatgagctcc cgagacgagg
     8101 accgcgagat ggcgtccttc taccggaggt acctcgacgc gatcgcctcg gcgggccagg
     8161 tcgacttcgc gggcctcctc ttccagagcg ccaaggtgct ggacgaggag gatcccgggt
     8221 accgccacgt gctcgtggac gactatcagg acgccacgca cgcggccgag gggatcctgc
     8281 gctcgctggc gtcgcgggcc gagagcgtgg tcgtggcggc cgacccgggc ggccacgtct
     8341 tctcctaccg aggagggacg ctggagccgt accggcgcct ggacgagacg ctgggtcggc
     8401 cggagcgcgt cgagctgccc gggagccgga ggctgtcgga ccagggccgg tcgctcgagg
     8461 agctggagcg agccgaagcg agcggggtcg aggcgccgcc ttggctcagc gcccgcctct
     8521 acgcgcaccc cggcgaggag gccgaggcga tcgcccacga gctgctccaa cggcgggtgg
     8581 acgaggacct cccctgggag gccatggccg tcgtgctccg ccgctacggc ggctacctga
     8641 ccgcgatccg gcacgcgctg gctcgccacc ggatcccctt cgtcgtcgtg gccgaggcgg
     8701 ccgagatcgc ggcggagccc gccaaccgtc cgatcgtgga cctcctgcgg ttcgtcttcc
     8761 gccccgagcg gcggggcgag ctgctcgagc cggtgctgac ctcgccgatc ggcggcctcg
     8821 atccccacga gacccggcgc ctgcgccggg aggcccggcg cgccgggacc ccgctgctcc
     8881 acctcgtcct cgaggcggcg ctcgaggacc tccccggccc gctgcgtggg cgggtgttgg
     8941 cgctccgcga gctcttcgat cggatgccgg cggtcgaaga ggagcgagga gccgacggcc
     9001 tcttccactg gctgtggacg cggctgcccc acgtccgcgg tctcgtggcc gaggaggggc
     9061 gccagcgcga cctcgacgcg ctcgcggcgc tggccggggt cctctcccga ttcgtcgagc
     9121 gccgcccggg ctcggggatc gaggactacc tggacactct cgacgccgcc ggcttcggcc
     9181 ccgatccctg gatcccgccc gaggagcggc atccccaggc ggtccggatc atctcggccc
     9241 accgcgcgca gggtctcgag ttcgacgtgg tcctcatcgc cgggtgtcta gaaggcgagt
     9301 tccccagcct aggggctcgc tcggccctcg tggacctgga gtcgatcgtg gacccgaagc
     9361 cccccaccga gcggctccgc gatcgcctgg ccgaggagcg ggccctgttc cggctcgccg
     9421 tctcgcgcgc ccgcctggcc acggtcctct tcgcatcgac ctccagcggg gcccgccgcc
     9481 ccaggcgtcc ctcccggttc gcggaacgtc tggggatcgt ctgggagccg ccggccgagg
     9541 tcgcgcctcc ggccgtatcg ctccgctcga tggaggcggc ccttcgcctc cgcctcgccg
     9601 atcccgaagc ccctcgccac gagcggttcg cggccctcgc cgccctcccc gcgatcgggg
     9661 ctcgaccctc cgcttggtgg cacggccgcg actggaccga tccggggcgc ccgatccggg
     9721 agggcgagtt ccagacgagc ttctcgcgcc tcgaggccct cgaggcctgc ggcctcctct
     9781 acctggggag gaccgagctc ggcctcgacc ccgacgccac ccactacatg tggctgggct
     9841 cggtcatcca cgagatcatc gagcgggtcc agaagggtga gctaccccgg gacgtcgacg
     9901 ccctggtcga ggcgctggac gcggcctggg agcccgagca gttcccccac cgggccgtcg
     9961 agcaccggcg acgcctcgac gccgaggcga tgctgcggcg ctgggtcgag cacgaccggc
    10021 gcgaggccat cgcctccgag cgagcgttcg agttcgagat cgccagggcc cgggtccggg
    10081 ggcgcatcga cgcgatcttc cagatgaaca acggcggcct ccacatcctc gactacaaga
    10141 gcgggaagaa cgcgcccacc cagaaggaca tcgagggcaa cctgcagctg gccacgtact
    10201 acctcggggt gaagcgcgac cccgagctgt cccagctcgg cgagccgaag tacctcgagc
    10261 tcgccttcct cggcaaggac cactacaagg acgggttcgt gaagcgcggc ttcatggcct
    10321 cgaaggtcga ggggtacgag gacctctcgg aggggaggct cgaggagctg atacggcggg
    10381 tccgggacga ggacttcaag cccgtggcca accccgagtg ccgcctgtgc gccctgcgca
    10441 cgatctgcgc catccagatc gagggcgggg aggcgccggt gtgagcgcgg cctccttcca
    10501 cgaggggacg cctcgcgaga tcgtcgaggc gctggggggc cggacgccga ccgtccagca
    10561 atgggaggcg atctcggccg gcctggagcc cgcagcggtc gtggccggcg ccggctcggg
    10621 gaagacggcg gtgatggccg ctcgggtcgt gtacctggcg ctggtgcacg ccgggaggct
    10681 tgaggccgac catccgggag ccccgccgtc gcgcgtcctc tgcctcacct tcacgaacaa
    10741 ggccgccgag gagctggccg atcgcgtggg acgcgcgacg gcggggctcg gcctccccga
    10801 tggcgaagag gcgaccgtcc tcacctacca ctcgttcgcc gcggccctga tcgacgagca
    10861 cggcatgcgg ctcggcctcc ccgctaccgc caggctcctc agcgaggggg agcgctggca
    10921 gatcgccgag cgcctcctcg acgatcgcga gctcgagtgg atccccaccg cgtgggtccc
    10981 ctcgctcgtc ggcgacacgc tcgcgctggc cgacgcctgc gccaaccagc tcatcgaccc
    11041 cgaggagctc gccgcccgcg agctcgccac cgtggaggcc ctgggcgagc ccgagggccg
    11101 tgtggacaag acccggcgca cgatcgggcg tcagcgctca gagctggctc ggatggtggc
    11161 cgacttccag gcggcgaagc gggagatggg cgtcgtggac tacggggacc agatcgcgca
    11221 cgccgcgaag ctggccaagg agcatcccga ggtgggggag tggttccgcg agcgctactc
    11281 ggtcgtcctc ctcgacgagt accaggacac gaacctcgcg caggccgacc tgatgcggca
    11341 cctcgtcggc cgggggttcc cgatcttcgc cgtgggcgac cccgaccaga acatctacgc
    11401 ctggcggggc gccacgctcc gcaacatcct cggcttcggc gacgacttcg gcgcctcgaa
    11461 cggcgagctg ccgctgtacg tgaacttccg ctccggctcc cggatcctcg ccgccgcgaa
    11521 caacgtgatc gagccggtcc cgccggagcg ccgggccgcc gggaagatcc tgcatcgcca
    11581 cgagcccttg ggggaggggc gcgtcctggg cttcgtggcc gaggatcagc gggccgaggc
    11641 cggacagatc gcccgcatga tcaaggagat gcggggctcg ccccgacgcg acggcggcgc
    11701 gctccagtat ggggacttcg tggtcctctg tcggaagaag cggctcttcg gtcccatcgc
    11761 ggaggtcctt cgccaggaga acctccctgt ggagatcgtg gacctcggcg gcctgctaca
    11821 gctccccgag atcgtggacg tcgtggcgtg gctgcggctg ctggagaacc ccggcgacaa
    11881 cgtctcgctg gcccgcatcc tcttcggccc gcgttggcgg atcggctacc gggacctcgc
    11941 catcctggcc cactggtcgg ccgaccagaa ccgctccctg gccgacgagc tcgatgcaga
    12001 ccggagcccc ggcgaggtcc ggttcgcgct ggtggaggcc gtggatcacc tcgacgagat
    12061 cgagggcctc tcgcccgagg cccgggagcg gctggaggag ttccggggcg acctcgcggg
    12121 gctccgagag cgggtgcgtc agcccctcgg ggacctcgtg gccgaggtgt tcgaggtctc
    12181 cggcctcctc cgcgagctcg aggcctcgac atcgccgccg gcccgcgccg ctcgccggaa
    12241 cctcctcaac ctcgtggatc acgtcgcggc gttcacctcg ctcgacggcg aggccaccct
    12301 ctccgcgttc atcgcctatc tgaacgccgc cctcgaggcc gaggacgagc tcgagcccgc
    12361 ccaggcctcc gagtccgaca ccgtgaagct catgacggtt cacaaggcca aggggctgga
    12421 gtggccggtc gtgttcgtcc cggggatggc cgagcacgag cgcccggcgt cgagcatctt
    12481 ccccaacttg cggacgcgcg agaaccccct gctcgggaag accgcgatcc ccctcgagat
    12541 gcggcgtgac gaggccgatt tccccgactt cgagggggac atcgacgctt ggggggcggc
    12601 cctccgggag cagcagctcg aggaggagcg ccgcctgggc tacgtcgcgc tcacgcgggc
    12661 tcaggagacg ctcgtggtct cggccgcgta ctggtacgag gggccccagg acccgtacgg
    12721 gccctcccgc ttcttcgacg aggtgaccgg gtcgcccggg gccgaggtcc tcttcgttgc
    12781 cgatcgaccc gacgagaacc cgatcgccgc cctgctcgcc gagaaggcgg ccgactggcc
    12841 gggcccgggg cgacgcgagg acgccgacga gctcttcccc ggagggtggc gcgccgccgc
    12901 gatcgatgcg gtcgggtccc cggacggcgc tgagcgccgc gtggagagcg tcctcggccc
    12961 tcgggacctg gaggccttcc gccgcctggt cgcctctcat cgtgagcgag cggcccgcat
    13021 cggggagcgc gtgacctccg aggggccgcc gccgctcccg gcggcgctct cggtctcgtc
    13081 ggtgatcgac ctcgagcgat gccccaagct cttctactgg tcccacgtgc ggcccctgcc
    13141 ccggcgaccg agcagcgccg cgcggctcgg gagcgaggtg caccggtgga tcgagctcca
    13201 gtccagtggc caggcttcgc ttctcgacgt ggacgagatc ccggacatgg gtacggagga
    13261 gaggatgggg gatccgggca tcgtcgccga gatgcggcgt gccttccgcg agagcaggtt
    13321 ctccgagagc gtgcccctct atgcggagcg tccgttcctc ttgtacctgg agggaagcgt
    13381 cgtgggcggc cggatcgacg cgatcttcgg ggagccccac ggaccctggg aggtggtcga
    13441 ctacaagact ggccggaagc ccgacgacga tccgctggcg ggcctccagc tcgacctgta
    13501 cgcgctcgca tgcgtcgaga tctggggaaa ggctcccgag gagctcacgc tgacctactt
    13561 ctacctggcc tcgggtgagg aggagacgcg cccggccggc gatcctgagg agacccggaa
    13621 gcgtgtcctc gaggcgatcc gacgggcagc atcgggcgag ttcgaggcca caccgggccc
    13681 gcagtgcagg tggtgcgact tccgagagtt ctgcgaaccg ggagccatct gggtccgaga
    13741 acacgggtga tacccatccc ccatccaggg gacaagattt caccctgcga gaaattgact
    13801 ctcgctcctc cccagcgcga cgattcccct gcgatggcaa cc
//
LOCUS       DASUTZ010000166         5605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_309172, whole genome shotgun sequence.
ACCESSION   DASUTZ010000166 DASUTZ010000000
VERSION     DASUTZ010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5605
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309172"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..1836
                     /locus_tag="VF097_07240"
     CDS             223..1836
                     /locus_tag="VF097_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262623.1"
                     /translation="MQRIRDPRAGRSGGRTVRLEGDDAGELERHLAENTEIIDERLED
                     GLAAIERSAAKLMRHLAAEVWRASGPDARKNLKDGVIAALTQDDAIRGLLSHTDERFQ
                     SLQIRVTRMEEAVRQLAEATRDAVKRVSERAREAEASGVAEEIARQRQDLARFSRRMT
                     EGMGEMYRRLQTGLRSEVADVREGIRGELQRLEAAVTSETAFSADAARQLREELRALV
                     DGTAERTGVVLEGIHADLTRRIAESMEGRARDIESLAERSDALRRDLDERLERLEAAQ
                     SRGSERMSEYAAQAAEGVAEMARRIQGEIAALAERAEEAERRAGERLGGLRGAIEESA
                     RRTSALTERVGRGLTEATARIREGFRMTLESAAEESRRTGEAMELALAREIVSLQEEV
                     AGVAARTGAVLQGIHADLADRIEGTAGSMREEAIHLVTQLVGRHAGQQGRSLQAITDS
                     LRALLDAVRKSGQDQSASIERVLRTSAARQEAALDESLTALRASVEELTGIYAREPRP
                     EDAAVFTRRLRGVERRLERLSRELAGDVA"
     gene            complement(1833..2198)
                     /locus_tag="VF097_07245"
     CDS             complement(1833..2198)
                     /locus_tag="VF097_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262624.1"
                     /translation="MRCDEVVAVLPAFAGEEAPYPHDLEVHLQTCRGCADEEIRYREL
                     LGIVRGLRDAGEPAPPGLALRVSRRAARLDVAWRGYARRLTRTPRSRYAAAGIGGAVV
                     GATAIALILRRGARRAATS"
     gene            complement(2188..2748)
                     /locus_tag="VF097_07250"
     CDS             complement(2188..2748)
                     /locus_tag="VF097_07250"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6262625.1"
                     /translation="MEGSKPLVDGELLDACRHGDPAAFEELVARTQHRVYALAYRLVG
                     DRSEAEDVAQEAYLRVFRSLGGFREESRFETWLHRIVVNAAMTHLRRRGRFGDLLRDP
                     VTDPEPPPAPPAQDRAADRDLIRRALEELPQGMRTALLLYEMYGLSCGEIGRELGLSE
                     GAVKIRLHRARKRLKEAIHGASEHAV"
     gene            complement(2782..3213)
                     /locus_tag="VF097_07255"
     CDS             complement(2782..3213)
                     /locus_tag="VF097_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018519331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX6262626.1"
                     /translation="MTVARRALEDVAVGDEIPERTLVVDRAQLVQYAGASGDFNPIHW
                     NPDFARMVGLPGVIAHGMFTMALVARAVGEWAGDAGAVRRISVQFRREVLPEEKVVAK
                     GRVAEKDEEARTVRLELWAELERDGKVLQPIKNGEAVVELS"
     gene            complement(3210..3659)
                     /locus_tag="VF097_07260"
     CDS             complement(3210..3659)
                     /locus_tag="VF097_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017593331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6262627.1"
                     /translation="MALNRELVGKEYPGVIYEVGREKIREFAQAVGETNPVYHDPEAA
                     RAAGHAEQVAPLTFPTVLHLRSGPQVVMDPELGLDYSLVVHGTQEFEYRRPILAGDVL
                     TATPRIAEITAKGPHEFLVIENTMTDPSGEVVCIARATLISRGTAER"
     gene            complement(3663..4469)
                     /locus_tag="VF097_07265"
     CDS             complement(3663..4469)
                     /locus_tag="VF097_07265"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX6262628.1"
                     /translation="MGRLDDRVAVITGAGRGIGRATAVRFAAEGAAVVVNDVDAEPAE
                     ETAQGVKDAGGEVLVSTHDTVDLEQARGMVEAAVERFGKLDVVVNNAGITRDRTFHNL
                     DDELWDLVLDVNLKTAFHTTLAAMPHLRGAAKRELGEQGKPSHHRKITFTTSVAALVG
                     NAGQANYTAAKGGIAALTRTLARELGPFQINVNAVAPGFIETRLTQAKQEGEDLGVPE
                     QIRQMALMLIALGRYGQPEDVANAHLFLASSEADYISGVILPVAGGQLGA"
     gene            4598..5293
                     /locus_tag="VF097_07270"
     CDS             4598..5293
                     /locus_tag="VF097_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6262629.1"
                     /translation="MASRRRREEQRIRILIADDHRSYAEALKTVIDLERGLMVTEVVT
                     DGRSAVDRVAVQRPDVVLMDVEMPVMDGIAATREIKRVFPEARIVVLAPDDNEVTLAR
                     AVEAGASGFVSKVRPVREVAEAVRAAFSGESEFDPEEVDRMLSVLGELREEDAAARAR
                     VERLTPRQIEILQLMADGLSPEEMATDLGISRHTLRTHVQNILTKLGVHSKLQALAVA
                     IRFGKVKARGPVA"
     gene            complement(5297..5461)
                     /gene="rpmG"
                     /locus_tag="VF097_07275"
     CDS             complement(5297..5461)
                     /gene="rpmG"
                     /locus_tag="VF097_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX6262630.1"
                     /translation="MPKSDNRPKITLACGECKRRNYATRKNKVNDRERIELRKYCRWC
                     RRHTLHKETR"
     gene            complement(5465..>5605)
                     /gene="tuf"
                     /locus_tag="VF097_07280"
     CDS             complement(5465..>5605)
                     /gene="tuf"
                     /locus_tag="VF097_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011435038.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX6262631.1"
                     /translation="EMVMPGDNTEMTVELIAPIAMEEGLRFAIREGGRTVGAGRVTKI
                     LE"
BASE COUNT          811 a         2018 c         1983 g          793 t
ORIGIN      
        1 ccggagggac tcgaaccccc aaccgccggt tttggagacc ggtgctctgc cagattgagc
       61 tacggccctc ggcaccggga gtatatcggt aacgcgggaa cctcagtata cgggctcctc
      121 gcggagagcg gcaaaggatc ccccgcgccg cgggtgcgtg accccctcgc ggggctgtcc
      181 tacactggcg ggagcctcgc gcggcccaga acgcgaggat acatgcagcg catccgcgac
      241 ccgcgggccg ggaggtccgg cgggcgcacg gtccggctcg agggcgacga cgcaggcgag
      301 ctggagcgcc atctcgccga gaacacggag atcatcgacg agcgcctcga ggacggtctg
      361 gccgcgatcg agcgctcggc cgccaagctg atgcgccacc tcgccgccga ggtgtggagg
      421 gccagcggcc ccgacgcccg caagaatctc aaggacggcg tgatcgcggc cctgacccag
      481 gacgacgcga tccgaggcct cctcagccac acggatgagc gcttccagtc gctccagatc
      541 cgcgtcacgc ggatggagga ggcggtgcgc cagctcgccg aagccacccg cgatgcggtg
      601 aagcgcgtct cggagcgagc gagggaagcg gaggcctcgg gagtggccga ggagatcgcg
      661 cgacagcggc aggacctggc ccgcttctct cggcggatga ccgagggcat gggcgagatg
      721 taccgccggc tccagaccgg gctccggagc gaggtggcgg acgtccgcga gggcatccgg
      781 ggagagctgc agcgcctcga ggccgcggtg acctccgaga cggccttctc ggccgacgcg
      841 gcgcgccagc tccgcgaaga gctgcgagcg ctcgtggacg ggacggccga gcgcaccggg
      901 gtcgttctcg aggggatcca cgcggacctc actcgccgca tcgcggagtc gatggagggg
      961 cgagcccgcg acatcgagtc gctggccgag cggtccgacg ctctccggcg ggatctcgac
     1021 gagcgtctgg agcggctcga ggccgcgcag agccggggga gcgagcggat gagcgaatac
     1081 gcggcccagg ctgcggaggg cgtggccgag atggcccgcc ggatccaggg cgagatcgcg
     1141 gccctcgccg agcgagccga ggaggccgag cggcgcgccg gcgagcgcct gggcgggctg
     1201 cggggcgcga tcgaggagtc ggcgcggcga acgtccgcgc tgaccgagcg cgtgggcagg
     1261 gggctgaccg aggccaccgc tcggatccgg gagggcttcc ggatgactct ggagagcgcg
     1321 gccgaggagt cgaggcggac cggggaggcg atggagttgg ccctcgcgcg ggagatcgtg
     1381 agcctccagg aggaggtggc aggggtcgcc gcccggacgg gggcggtgct ccaggggatc
     1441 cacgccgacc tggccgaccg catcgagggg acggccggct cgatgcgcga ggaggcgatc
     1501 cacctcgtga cgcagctcgt cgggcgccac gccggacagc agggtcgcag cctccaggcg
     1561 atcaccgact cgctccgggc gctgctcgac gcggtccgca aatcgggcca ggatcagtcg
     1621 gcatcgatcg agagggtgct gcggacctcc gccgcgagac aggaggctgc cctggacgag
     1681 tcgctcacgg cgctccgcgc ctccgtcgag gagctgaccg ggatctacgc gcgcgagccc
     1741 cgacccgaag atgccgccgt gttcacgcgc cgcctgcgcg gcgtggagcg ccgcctcgag
     1801 cgtctctccc gggagctcgc cggcgacgtg gcctaggacg tcgccgcgcg acgagcgcct
     1861 cgacgcagga tcagggcgat cgcggtggcg ccgacgacgg ccccgccgat ccccgccgcc
     1921 gcgtaacggg atcgaggggt gcgcgtcagc cggcgcgcgt agccgcgcca ggccacgtcg
     1981 agccgggccg cgcgccggct gacgcgcagg gccagccctg gaggggcagg ctcgccggcg
     2041 tcgcggagcc ctcggacgat ccccagcagc tcccgatagc ggatctcctc gtcggcgcag
     2101 ccccggcagg tctggaggtg cacctcgagg tcgtgcgggt agggcgcctc ctcccccgcg
     2161 aaggcgggga ggacggccac cacctcgtca caccgcatgc tcgctcgcgc catggatcgc
     2221 ttccttcagt cgcttcctcg ccctgtggag ccggatcttc acggcccctt ccgagagccc
     2281 tagctcccga ccgatctccc cgcagctcag cccgtacatc tcgtagagga ggagcgccgt
     2341 cctcatcccc tgcggaagct cctcgagggc ccgacggatc aggtcgcgat cggccgcccg
     2401 gtcctgcgcc ggcggggccg ggggcggctc cggatcggtc acgggatccc gcaggaggtc
     2461 gccgaaccgg ccccgccggc gcagatgcgt catcgccgcg ttgacgacga tccggtgcag
     2521 ccaggtctcg aacctcgact cctcccggaa gccgccgagc gaccggaaca cccgcaggta
     2581 cgcctcctgg gccacgtcct cggcctcgct tcggtctccc accagccggt aggccagcgc
     2641 gtacacgcga tgctgcgtcc gcgccacgag ctcctcgaac gccgccgggt caccatggcg
     2701 gcacgcatcc agcagctccc cgtcgaccag cggtttggac ccctccatcc cccggcaggt
     2761 taccgcgctc ggggggtccg ttcaggacag ctcgacgacc gcctcaccgt tcttgatcgg
     2821 ctgaagcacc ttcccgtcgc gctccagctc cgcccacagc tccaaccgga ccgttcgcgc
     2881 ctcctcgtcc ttctccgcga ccctcccctt ggccaccacc ttctcttcgg ggaggacctc
     2941 cctgcggaac tgcacggaga tgcgcctcac ggcgccggcg tcgccggccc actccccgac
     3001 ggccctggcc accagcgcca tcgtgaacat gccgtgggcg atcaccccgg gcaggccgac
     3061 catcctggcg aagtccgggt tccagtggat cgggttgaag tcgccggacg cgcccgcgta
     3121 ctggaccagc tgggcccggt ccacgaccag ggtccgctcg gggatctcgt cccccaccgc
     3181 cacgtcctcg agggccctgc gcgccaccgt catcgctccg cggtcccccg ggagatgagc
     3241 gtagcccgag cgatgcaaac cacctccccc gacgggtcgg tcatcgtgtt ctcgatcacc
     3301 aagaactcgt ggggcccctt cgcggtgatc tcggcgatcc gaggggtcgc cgtcaacacg
     3361 tcgccggcca ggatcgggcg ccggtactcg aactcctggg tcccgtgcac gaccagcgag
     3421 tagtccagcc ccagctcagg gtccatcacg acctgcgggc ccgaccggag atgcagcacg
     3481 gtggggaagg tcagcggggc cacctgctcg gcatgccccg cggccctcgc ggcctcgggg
     3541 tcgtggtaga ccgggttcgt ctctccgacc gcctgcgcga actcgcggat cttctctcgg
     3601 ccgacctcgt agatcacccc cgggtactcc ttccccacga gctcacggtt gagcgccacg
     3661 cgtcaggctc ccagctgtcc gccggccacg gggaggatca cgccggagat gtagtcagcc
     3721 tccgagctgg cgaggaacag gtgcgcgttc gccacgtcct ccggctgacc gtaccgaccc
     3781 agcgcgatca gcatcagggc catctgacgg atctgctcgg ggacgcccag atcctcgcct
     3841 tcctgcttcg cctgcgtgag gcgcgtctcg atgaaccccg gggcgaccgc gttcacgttg
     3901 atctggaagg gtccgagctc gcgggccagg gtcctggtca gcgccgcgat cccgcccttc
     3961 gccgccgtgt agttcgcctg gcccgcgttg cccacgaggg ccgccaccga cgtggtgaag
     4021 gtgatcttcc ggtgatggct gggcttcccc tgctcgccca gctccctctt cgccgctccc
     4081 cgcaggtggg gcatcgcggc cagggtcgta tggaaggccg tcttgaggtt cacgtcgagc
     4141 acgaggtccc agagctcatc gtcgaggttg tggaaggtgc ggtccctcgt gatccccgcg
     4201 ttgttcacca cgacgtcgag cttgccgaag cgctccaccg ccgcctcgac catccccctc
     4261 gcctgctcga ggtccacggt gtcgtgggtg gagacgagga cctctccgcc cgcatccttg
     4321 accccctgag cagtctcctc ggccggctcc gcgtccacat cgttcacgac gacggccgcg
     4381 ccctcggcgg cgaagcgcac cgcggtggcc ctgccgatgc ctcgcccggc tcccgtgatg
     4441 acggcgaccc tgtcgtccag acgtcccaag cggctcctcc tcgcggcccg gtgaaggcta
     4501 ccgaagggta ggtacgaggg tcaaggcggg gacctgtgga gacgagatcc gccgcggtgc
     4561 tggcaacggc ggagcatggg tgcgaggatt cccggtgatg gcgtcacgga gacggcgcga
     4621 ggagcagcgg atccgcatcc tcatcgccga cgatcaccgg agctacgccg aggcgctgaa
     4681 gaccgtcatc gacctcgagc ggggcctgat ggtgaccgag gtggtgacgg acggccgctc
     4741 ggcggtggac cgggtcgcgg tccagcgacc cgacgtcgtg ctgatggacg tcgagatgcc
     4801 ggtgatggac ggcatcgccg cgacccgcga gatcaagcgg gtgttccccg aggctcgcat
     4861 cgtggtgctc gcgcccgatg acaacgaggt cacgctggct cgcgcggtcg aggcaggagc
     4921 ctccgggttc gtctcgaagg tgcggccggt gcgcgaggtg gccgaggccg tccgcgcggc
     4981 cttctcgggg gagtccgaat tcgacccgga ggaggtcgac aggatgctgt ccgttctcgg
     5041 ggagctccgg gaggaggacg ccgcggccag ggcccgcgtg gagcgcctca ccccgagaca
     5101 gatcgagatc ctccagctga tggccgacgg cctctctccg gaggagatgg ccaccgacct
     5161 ggggatcagc cgtcacaccc tccggaccca cgtccagaac atcctgacca agctcggggt
     5221 ccactcgaag ctccaagccc tggcggtggc gatccggttc gggaaggtca aggcccgagg
     5281 gcccgtggcc tgagcgctac cgcgtttcct tgtggagggt gtggcgccgg caccaccggc
     5341 agtacttccg cagctcgatc cgctcccgat cgttgacctt gttcttccgg gtcgcgtagt
     5401 tgcgccgctt gcactcgccg caggccagcg tgatcttcgg tcggttgtcg gacttgggca
     5461 ccggctactc caggatcttc gtcacgcgcc cggcgcccac ggtgcggccg ccttcgcgga
     5521 tggcgaagcg cagcccctcc tccatggcga tgggggcgat cagctcgacg gtcatctcgg
     5581 tgttgtcccc gggcatcacc atctc
//
LOCUS       DASUTZ010000167         7580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_697931, whole genome shotgun sequence.
ACCESSION   DASUTZ010000167 DASUTZ010000000
VERSION     DASUTZ010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7580
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697931"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VF097_07285"
     CDS             complement(<1..342)
                     /locus_tag="VF097_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX6262632.1"
                     /translation="MLRAFIDAFKIPDLRNKILFTLFIIAVYRIGAHWPVPNVDVAAA
                     RSATQVTGGQGNVLSFIDLFAGGALQNFAIFALGIMPYITSSIIMQLLTVVIPKLEQW
                     QKQGEVGVKKIT"
     gene            complement(398..835)
                     /gene="rplO"
                     /locus_tag="VF097_07290"
     CDS             complement(398..835)
                     /gene="rplO"
                     /locus_tag="VF097_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008287615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX6262633.1"
                     /translation="MKLHHLKPPKGAKKDRTRVGRGRAGARGKTAGRGTKGYLARNQP
                     KQGFEGGQMPLARRVPKKKGFTNRNREAWAVVNVETLAARFEAGETVTPETMAERGLV
                     RRRLPVKVLGAGEIDRALTIQAHAVSKGAREKIEGAGGTVEVL"
     gene            complement(832..1014)
                     /gene="rpmD"
                     /locus_tag="VF097_07295"
     CDS             complement(832..1014)
                     /gene="rpmD"
                     /locus_tag="VF097_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019818483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX6262634.1"
                     /translation="MAKLKVTQVRGRAGKPEPQRRTLIALGLRRVRQSVVHEDRPEIR
                     GMIAKVRHLVDVEEVR"
     gene            complement(1001..1600)
                     /gene="rpsE"
                     /locus_tag="VF097_07300"
     CDS             complement(1001..1600)
                     /gene="rpsE"
                     /locus_tag="VF097_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX6262635.1"
                     /translation="MRRYDSQDKSPYEERVVSINRVAKVVKGGRRFSFAALVVVGDTA
                     GRVGVGYGKAKEVPAAIQKGVEEAKRAMFEVPMMGTTIPHQVVGADGAGRVLLKPAAP
                     GTGVIAGGPVRAVIEAAGIKDVLSKSLGSDNPINIVHATVKGLRELRRPEDVAALRER
                     EIHEVAPRPMIEAMEAARTRLAHERREAGLDREEAGGEA"
     gene            complement(1597..1962)
                     /gene="rplR"
                     /locus_tag="VF097_07305"
     CDS             complement(1597..1962)
                     /gene="rplR"
                     /locus_tag="VF097_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX6262636.1"
                     /translation="MTTRTAKTRAARVRRHRRVRKRITGTAERPRLAVYRSNRHVYAQ
                     LVDDVSGRTLASASSLEPEVRDGGMGKTEVAKVVGGLIAGRAKEAGIERAVFDRGGFL
                     FHGRVAAVAEGAREGGLQI"
     gene            complement(1959..2510)
                     /gene="rplF"
                     /locus_tag="VF097_07310"
     CDS             complement(1959..2510)
                     /gene="rplF"
                     /locus_tag="VF097_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015755975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX6262637.1"
                     /translation="MSRIGKEPIEIPDGVTVTLEGSRVSVEGPKGSLHQDVDPDMRVT
                     VEDGRVTVERPSDVREHRALHGLTRSLIYNMVHGVTEGYQKVLEIQGVGYRATLQGNA
                     LEVQVGYSHPVRVEPPEGIEFEVPAQNRIVVRGIDKQLVGQVAADIRDIRKPEPYKGK
                     GIRYEGEYVRKKAGKAAKAGLGA"
     gene            complement(2515..2916)
                     /gene="rpsH"
                     /locus_tag="VF097_07315"
     CDS             complement(2515..2916)
                     /gene="rpsH"
                     /locus_tag="VF097_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX6262638.1"
                     /translation="MTMTDPVADMLARIRNAAQAHQDEVAIPASGVKEQIARVLRDEG
                     YVRGYRIDESEGPKNRIVIEMKYGPERQRAITGMRRVSRPGRRIYAGRDRLPRVQGGL
                     GVAILSTSKGVMTDRRAAREGVGGEVLCYVW"
     gene            complement(2924..3109)
                     /locus_tag="VF097_07320"
     CDS             complement(2924..3109)
                     /locus_tag="VF097_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEX6262639.1"
                     /translation="MARKALMNRAKARPKYRSRALTRCERCGRARAVFRRFRLCRLCF
                     RELAHRGEIPGVTKASW"
     gene            complement(3120..3710)
                     /gene="rplE"
                     /locus_tag="VF097_07325"
     CDS             complement(3120..3710)
                     /gene="rplE"
                     /locus_tag="VF097_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003149758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX6262640.1"
                     /translation="MREGSLMAPRLKDWYRETLVAKLQKELGLSNPMEVPRLEKIVIN
                     MGLGEASRDVKVLDGAVEDLRTITGQQPVITKARQSIAGFKLREGMPIGAKVTLRGDR
                     MWEFLDRMLSTALPRIRDFRGLDPEAFDGHGNFTIGVTEQLIFSEVDYDQIDAVRGMD
                     ITIVTTAATDEEGRALLRVLGFPFQGLPVEPVMGAA"
     gene            complement(3692..4006)
                     /gene="rplX"
                     /locus_tag="VF097_07330"
     CDS             complement(3692..4006)
                     /gene="rplX"
                     /locus_tag="VF097_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX6262641.1"
                     /translation="MPVAIRKDDEVLVISGKDRGKRGRVVNVLPREGRVMVEGIARAK
                     KHARPSRKRQQGGIVDIEQFVDISNLMLVCRSCGQPTRVGHRFDEDGGKVRVCKRCGK
                     DL"
     gene            complement(4006..4374)
                     /gene="rplN"
                     /locus_tag="VF097_07335"
     CDS             complement(4006..4374)
                     /gene="rplN"
                     /locus_tag="VF097_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX6262642.1"
                     /translation="MIQQESRVKVADNTGAKEVLCIRVLGGSARRYAGVGDIIVGTVK
                     DALPGAGVKKGDIVRAVVVRAAKERRRSDGSYIKFDDNAVVLINETNAPRGTRIFGPV
                     ARELREKRFMKIVSLAPEVL"
     gene            complement(4371..4631)
                     /gene="rpsQ"
                     /locus_tag="VF097_07340"
     CDS             complement(4371..4631)
                     /gene="rpsQ"
                     /locus_tag="VF097_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005253856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX6262643.1"
                     /translation="MSAERGSRKVRTGVVVSNAMDKTVVVRVDGQLRHPLYRKIVRRT
                     SKLRAHDEGNEVGVGDRVRIMETRPLSKTKRWRVVEIVEKAK"
     gene            complement(4628..4867)
                     /gene="rpmC"
                     /locus_tag="VF097_07345"
     CDS             complement(4628..4867)
                     /gene="rpmC"
                     /locus_tag="VF097_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013805286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX6262644.1"
                     /translation="MTKPAELRDLDRNELLERLEAAKEELFNLRFQFATGQLDNPMTI
                     KAVRHDMARILTILRERELEELEAEEAAGEEEGAR"
     gene            complement(4864..5283)
                     /gene="rplP"
                     /locus_tag="VF097_07350"
     CDS             complement(4864..5283)
                     /gene="rplP"
                     /locus_tag="VF097_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX6262645.1"
                     /translation="MLQPKKVKWRKQQRGRMRGKAKGGTDVQFGDYGLAAGEPGWISN
                     RQIEAARVAITRYIRRGGKVWINIFPDKPVTKKPAETRMGSGKGNPEDWVAVVKPGRV
                     MFELSGVSEPVAREALRRGAAKLSVRSKFVTRFGGGA"
     gene            complement(5283..6119)
                     /gene="rpsC"
                     /locus_tag="VF097_07355"
     CDS             complement(5283..6119)
                     /gene="rpsC"
                     /locus_tag="VF097_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009306350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX6262646.1"
                     /translation="MGQKINPYGFRLGVIYPWRSKWYAGKKEYAQALHEDIKIRDYIK
                     LHLSRAGISEIIIDRKGGDQIEVNIRTARPGIVIGRKGSEVERVRKDLERITAKRVSV
                     NVEDLNVAATDSRPETDATLLAQGVAEQLKGRVSFRRAMRRAVQTAMRAGALGVRVQC
                     SGRLGGMEMSRREWYREGRVPLHTLRAKIDYGLAEARTTFGMIGVQVWVYHGDEIPQE
                     EKETERARARALAGIGGASTGALVTDEKEMAEVAEEPGSAKPADGDEATSKEGAPQGG
                     SA"
     gene            complement(6103..6462)
                     /gene="rplV"
                     /locus_tag="VF097_07360"
     CDS             complement(6103..6462)
                     /gene="rplV"
                     /locus_tag="VF097_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEX6262647.1"
                     /translation="MEARASSKYVRVAPRKARRVIDLIRGRHVEEARRILRFTPYGVN
                     QTVAKTLESAVANAGQLPGVVPENLFVTKAYVDEGPTLRRFRPRAMGRATRIDKRTSH
                     ITVIVGTTEEVPGGPED"
     gene            complement(6468..6752)
                     /gene="rpsS"
                     /locus_tag="VF097_07365"
     CDS             complement(6468..6752)
                     /gene="rpsS"
                     /locus_tag="VF097_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEX6262648.1"
                     /translation="MPRSLKKGPFVDDHLLAKVEAMNQRGEKRVIRTWSRRSTIIPEM
                     VGHTIAVHDGRKHVPVFITEGMVGHKLGEFAPTRTFRTHGYHTERSTSLK"
     gene            complement(6756..>7580)
                     /gene="rplB"
                     /locus_tag="VF097_07370"
     CDS             complement(6756..>7580)
                     /gene="rplB"
                     /locus_tag="VF097_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX6262649.1"
                     /translation="VRKHKPTTPGRRGSSVSDFSEITKDRPERSLTKPLPKKAGRNTY
                     GRITSRRRGGGHKRRYRVIDFRRNKDGVPAKVAAVEYDPNRSARIALLHYADGEKRYI
                     LAPQGLAVGDTVTSGPGADIKPGNALPLANIPVGTIVHAVELRPGGGAKMARSAGSSI
                     QIMAKEGKHAQLRMPSGEMRLVEAQCRASVGAVGNPEHELISWGKAGRTRWRGKRPKV
                     RGVAMNPVDHPLGGGEGKSSGGRHPTSPWGKKEGRTRKKGKASDRYIIRRRGKGRG"
BASE COUNT          962 a         2845 c         2442 g         1331 t
ORIGIN      
        1 ggtgatcttc ttcaccccga cctcgccctg cttctgccac tgctcgagct tcgggatcac
       61 cacggtgagg agctgcatga tgatcgagct cgtgatgtag ggcatgatcc cgagcgcgaa
      121 gatcgcgaag ttctggagcg cacccccggc gaagaggtcg atgaacgaga ggacgttccc
      181 ctggcctccc gtgacctgcg tcgcggagcg ggccgcggcc acatcgacgt tggggacggg
      241 ccagtgcgcg ccgatccggt acaccgcgat gatgaagagc gtgaagagga tcttgttccg
      301 gagatccggg atcttgaacg cgtcgatgaa cgctcgcagc acgtctgctc ctcctcgccg
      361 gcccgcccgg cgaactcggc gcctagtcta tcggaggcta gaggacttcg accgtcccgc
      421 ccgcaccctc gatcttctcg cgggcgccct tcgagacggc gtgcgcctgg atcgtcagcg
      481 cgcggtcgat ctcgccggct ccgaggacct tcacggggag gcggcgccgg accagccccc
      541 gctccgccat cgtctccggg gtgacggtct cgcccgcctc gaagcgagcg gccagcgtct
      601 cgacgttgac caccgcccac gcctcgcggt tccggttcgt gaaccccttc ttcttgggaa
      661 ccctgcgggc caggggcatc tgcccgccct cgaacccctg cttgggctgg ttccgagcga
      721 ggtagccctt cgtgccgcgg ccggccgtct tgcctcgggc gccggctcgc cctcgaccca
      781 cccgggtccg gtccttcttg gcgcccttcg gcggcttgag gtggtgcagc ttcatcggac
      841 ctcctcgacg tccaccaggt ggcggacctt ggcgatcatg cctcggatct ccgggcggtc
      901 ctcgtggacg accgactggc gcacccggcg gagccccagc gcgatcaggg tccgccgctg
      961 aggctcgggc ttgcccgccc gcccccgcac ctgagtgacc ttaagcttcg ccaccggcct
     1021 cctcccgatc caggccggcc tcccgccgct cgtgggccag gcgcgtgcgg gcggcctcca
     1081 tcgcctcgat catggggcga ggggccacct cgtggatctc gcgctcacgc agggcggcca
     1141 cgtcctcggg gcgtcggagc tcgcgaagcc ccttcacggt ggcgtgcacg atgttgatcg
     1201 ggttgtcgga gcccagcgac ttcgagagca cgtccttgat cccggccgcc tcgatcacgg
     1261 cgcgcacggg cccgccggcg atgacgcccg tgcccggcgc tgcgggcttg agcaggacgc
     1321 ggcccgcccc gtccgctccc acgacctggt gcgggatcgt ggtccccatc atcgggacct
     1381 caaacatggc gcgcttggcc tcctccacgc ccttctggat cgccgcgggg acctccttgg
     1441 cctttccgta gccgacccct acgcgtccgg ccgtgtcgcc cacgaccacg agggccgcga
     1501 acgagaagcg ccggcccccc ttcacgacct tggccacgcg gttgatggag accacgcgct
     1561 cttcgtaggg gctcttgtcc tggctgtcgt agcgcctcaa atctggagtc ctccctctcg
     1621 ggccccctcg gccacggccg ccacgcgacc gtggaagagg aagccgccgc ggtcgaagac
     1681 ggcgcgctcg atgcccgcct ccttggcccg gccggcgatg aggccgccca cgaccttcgc
     1741 cacctcggtc ttgcccatcc caccatcccg gacctccggc tcgagcgagg aggccgaggc
     1801 cagcgtgcgg cccgagacgt cgtccacgag ctgcgcgtag acgtggcggt tcgagcggta
     1861 caccgcgagg cgtggcctct cggcggtgcc ggtgatccgc ttccggaccc gtcggtgccg
     1921 gcggacccgg gcggccctcg tcttcgcggt tctggtcgtc atgcccccag ccccgccttc
     1981 gcggccttcc cggctttctt ccgcacgtac tcgccctcgt agcggatgcc cttgcccttg
     2041 tacggctccg gcttccggat gtcgcggatg tcggccgcga cctgtcccac gagctgcttg
     2101 tcgatccccc gcaccacgat gcggttctgg gcggggacct cgaactcgat gccctcgggg
     2161 ggctcgaccc gcaccggatg cgagtagccc acctggacct cgagcgcgtt cccctggagc
     2221 gtcgcccggt agcccacgcc ctggatctcg agcaccttct ggtagccctc ggtgacgccg
     2281 tggaccatgt tgtagatcag cgatcgggtc aggccgtgca gggcccggtg ctcccgcacg
     2341 tccgaggggc gctcgaccgt cacgcggccg tcctcgacgg tcacgcgcat gtcggggtcc
     2401 acgtcctggt ggagcgaccc cttgggcccc tccacggaga cgcgcgagcc ctccagcgtc
     2461 accgtgacgc cgtcggggat ctcgatgggc tccttgccga tcctgctcat ctcgtcacca
     2521 cacgtagcag aggacttcgc cgcccacgcc ctcgcgagcg gcacgtcggt cggtcatcac
     2581 acccttcgag gtggagagga tcgccacccc gaggcctccc tggacccgtg ggagccgatc
     2641 ccggccggcg tagatccgcc ggcccggtct cgacacccgc cgcatgcctg taatcgcgcg
     2701 ctggcgctcg ggcccgtact tcatctcgat cacgatccgg ttcttgggac cctccgactc
     2761 gtcgatccgg tagccgcgaa cgtagccctc gtcgcggagg acgcgcgcga tctgctcctt
     2821 cacgccggaa gccgggatcg ccacctcgtc ctggtgcgcc tgggcggcgt tccggatcct
     2881 tgccagcatg tctgcgacgg ggtcggtcat cgtcatcgcg tcatcaccac gaggccttcg
     2941 tcacgccggg gatctcgccc cggtgagcga gctcacggaa gcagaggcgg cagagccgga
     3001 agcggcggaa gaccgcccgc gcccggccgc accgctcgca gcgggtcagc gcccgcgacc
     3061 ggtacttcgg cctcgccttc gccctgttca tcaacgcctt acgcgccatc tcgctcccct
     3121 cacgccgcgc ccatcacggg ctccacgggc agcccctgga aggggaaccc gaggacccgc
     3181 aggagcgcgc gtccttcttc gtcggtggcc gcggtggtca cgatcgtgat gtccatcccg
     3241 cgcaccgcgt cgatctggtc gtagtcgacc tccgagaaga tcagctgctc ggtcaccccg
     3301 atcgtgaagt tgccgtggcc gtcgaaggcc tcggggtcga ggccccggaa gtctcggatc
     3361 ctcggcagcg ccgtggagag cattcggtcg aggaactccc acatccgatc gccgcggagg
     3421 gtgaccttag cgccgatcgg catcccctcg cggagcttga aaccagcgat cgactgtcga
     3481 gccttcgtga tcaccggctg ctgccccgtg atcgtgcgga ggtcctccac cgcgccgtcg
     3541 agcaccttga cgtcgcggga ggcctcgccc aggcccatgt tgataacgat cttctccagc
     3601 cggggcacct ccatcgggtt ggagagaccg agctccttct gcagcttggc cacgagggtc
     3661 tcccggtacc agtccttcag cctgggagcc atcagagatc cttcccgcat cgcttgcaca
     3721 cccgcacctt gccgccgtcc tcgtcgaatc ggtggccgac cctcgtgggc tggccgcacg
     3781 accggcacac cagcatcagg ttggatatgt ccacgaactg ctcgatgtcc acgatcccgc
     3841 cctgctggcg cttgcgcgag ggccgggcgt gcttcttggc ccgggcgatg ccctccacca
     3901 tgacgcggcc ctcccggggc aggacgttca ccacgcggcc tcgcttgccg cggtccttcc
     3961 ccgagatcac caggacctcg tcgtccttcc tgatcgccac cggcatcaca ggacctccgg
     4021 cgcgagcgac acgatcttca tgaacctctt ctccctgagc tccctggcca cgggaccgaa
     4081 gatgcgggtc ccgcgcggcg cgttcgtctc gttgatgagc acgacggcgt tgtcgtcgaa
     4141 cttgatgtag gagccgtcgg agcggcgtcg ctccttcgcg gccctgacca cgacggcccg
     4201 gacgatgtcg cccttcttca ccccggctcc ggggagggcg tccttcaccg tcccgacgat
     4261 gatatcgccc acaccggcgt accgacgggc cgagccgccg agcacgcgga tgcacaggac
     4321 ctccttggcc cccgtgttgt ccgcgacctt gacccgggac tcctgctgga tcacttggcc
     4381 ttctccacga tctcgaccac gcgccagcgc ttcgtcttgg acaggggacg ggtctccatg
     4441 atgcggaccc ggtcgcccac gccgacctcg tttccctcgt cgtgggcccg gagcttcgac
     4501 gtccgccgaa cgatcttccg gtacagaggg tggcggagct gcccgtcgac gcgcaccacg
     4561 acggttttgt ccatcgcgtt cgagacgacg acgcccgtcc gcaccttcct gctgccgcgc
     4621 tcggcgctca tcgggcaccc tcctcctcgc ccgcagcctc ctcggcctca agctcctcga
     4681 gctcgcgctc gcgaaggatc gtcaggatcc gggccatgtc gtgacggacc gccttgatcg
     4741 tcatcgggtt gtcgagctgg ccggtggcga actggaagcg gaggttgaac aactcctcct
     4801 tcgccgcctc cagccgctcc agcagctcgt tccggtccag gtcacgaagc tcggcgggct
     4861 tcgtcacgcc ccacccccga acctggtcac gaacttgctg cggacggaga gcttcgcggc
     4921 gccgcggcgc agggcctcgc gggccacggg ctcgctgacg ccggagagct cgaacatcac
     4981 gcgtccgggc ttgaccacgg ccacccagtc ctcggggttg cccttccccg agcccatacg
     5041 ggtctcggcc ggcttcttcg tcacgggctt gtcggggaag atgttgatcc acaccttgcc
     5101 gccgcgtcgg atgtagcggg tgatggccac gcgggccgcc tcgatctggc ggttgctgat
     5161 ccagcccggc tccccggctg ccaggccgta gtccccgaac tgcacgtcgg tgccgccctt
     5221 ggccttgccc cgcatccggc cccgctgctg cttccgccac ttgactttct tcggctgcag
     5281 catcaggccg atcctccctg gggggcgccc tctttcgagg tggcctcgtc gccatcggcg
     5341 ggcttcgccg acccgggctc ctcggccacc tccgccatct ccttctcgtc ggtgaccagc
     5401 gcgccggtcg aggcgccgcc gatgccggcc agggcccggg cgcgggcccg ctccgtctcc
     5461 ttctcctcct gcgggatctc gtccccgtgg tagacccaga cctggacccc gatcatgccg
     5521 aaggtggtgc gggcctcggc cagcccgtag tcgatcttgg ctcggagggt gtggagaggg
     5581 acacgcccct ctcggtacca ctcgcggcgc gacatctcca tgccgcccag acggccgctg
     5641 cactgcacgc ggacgcccag ggccccggcg cgcatcgcgg tctgcacggc ccggcgcatg
     5701 gcccggcgga acgagacgcg gcccttgagc tgctcggcca ccccctgcgc caggagggtg
     5761 gcgtcggtct cgggacggga gtcggtggcc gccacgttga ggtcctcgac gttgacggac
     5821 acgcgcttcg cggtgatccg ctcgaggtcc ttgcggaccc gctccacctc ggaccccttc
     5881 cggccgataa cgatccccgg ccgcgccgtc cggatgttca cctcgatctg gtcgccgccc
     5941 ttgcggtcga tgatgatctc ggagatcccg gcccgcgaca ggtggagctt gatgtagtcg
     6001 cggatcttga tgtcctcgtg gagcgcctgc gcgtactcct tcttgcccgc gtaccacttc
     6061 gagcgccacg ggtagatcac gccgaggcgg aagccgtagg ggttaatctt ctggcccacc
     6121 gggcacctcc tcggtcgtcc ccacgatcac agtgatgtgg ctggttcgct tgtcgatcct
     6181 cgtggcccgc cccatcgcgc ggggacggaa ccggcgcagg gtcggcccct cgtccacgta
     6241 ggccttcgtg acgaacaggt tctcgggtac gacgcccggc agctgcccgg cgttggccac
     6301 agccgattcg agggtcttgg ccacggtctg gttgacgccg tagggcgtga agcgcaggat
     6361 ccgcctcgcc tcctccacgt gcctcccgcg gatgaggtcg atgacccggc gcgccttcct
     6421 cggcgcgacc ctcacgtact tgctgctcgc tctcgcttcc atcgctccta cttcagggac
     6481 gtgcttcgct cggtgtggta gccgtgcgtg cggaaggtcc gcgtgggcgc gaactcgccc
     6541 agcttgtggc ccaccatgcc ctcggtgatg aacaccggga cgtgcttcct gccgtcgtgg
     6601 acggcgatcg tgtgcccgac catctcgggg atgatcgtcg agcggcggga ccacgtgcgg
     6661 atcacccgct tctcgccgcg ctggttcatc gcctccacct tggctaggag gtggtcgtcc
     6721 acgaacgggc ccttcttcag cgacctcggc atgcctcagc ccctcccctt gccgcggcga
     6781 cggatgatgt agcggtccga ggccttcccc ttcttgcggg tccgcccctc cttcttgccc
     6841 caggggctcg tgggatggcg gccgcccgag gactttccct cgccgcctcc caggggatgg
     6901 tccacggggt tcatggccac gccacgcacc ttgggccgct tgccccgcca gcgggtccgg
     6961 ccggccttcc cccacgagat cagctcgtgc tcgggattgc ccaccgcgcc gaccgaggct
     7021 cggcactggg cctcgaccag tcgcatctcg ccggagggca tccggagctg ggcgtgcttg
     7081 ccctccttgg ccatgatctg gatcgaggag ccggccgagc gggccatctt ggcgccccca
     7141 ccgggacgga gctccaccgc gtgcacgatc gtccccacgg ggatgttggc cagcgggagg
     7201 gcgttccccg gcttgatgtc ggccccgggg cccgaggtca ccgtgtcgcc caccgcgagc
     7261 ccctggggcg cgaggatgta gcgcttctcg ccgtccgcgt agtggagcag ggcgatccgc
     7321 gcgctccggt tggggtcgta ctcgaccgcc gcgaccttcg ccgggacccc gtctttgttg
     7381 cgccggaagt cgatcacgcg gtagcggcgt ttgtggccgc cccctctccg ccgagacgtg
     7441 atgcggccgt acgtgttccg gcccgccttc ttcggcagcg gcttggtgag cgaccgctcg
     7501 ggccgatcct tggtgatctc cgagaagtcg gagaccgagg acccgcgcct tccgggcgtc
     7561 gtcggcttgt gcttcctgac
//
LOCUS       DASUTZ010000168         6574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1345075, whole genome shotgun sequence.
ACCESSION   DASUTZ010000168 DASUTZ010000000
VERSION     DASUTZ010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6574
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345075"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VF097_07375"
     CDS             <1..673
                     /locus_tag="VF097_07375"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="catalyzes the conversion of salicylyl-CoA to
                     gentisyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HEX6262650.1"
                     /translation="IGHAGRRGSTRPRHRGLDLPLGASGWPLLSASPLPYTSVSPAPR
                     EMDRADMDRIRDAFVRAARAATDAGADVLGIHLGHGYLLASFLSPLTNVREDGYGGSL
                     EGRARFPLEVVEAVRAAWPENRPLWGTVTVDDWKRGGLSPMEAIELCAMLRDRGCDVL
                     EPVAGQTTPDSRPTFGRMFLAPYADRVRNEVGVATMTSGGITTTGQVNTLVAGGRADL
                     CVMDP"
     gene            670..1581
                     /locus_tag="VF097_07380"
     CDS             670..1581
                     /locus_tag="VF097_07380"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017753412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX6262651.1"
                     /translation="MSKLASMRDAVSEHVSDGSSVVMGTCLESMIPFAAGHELIRQER
                     RELTLIGPISDILFDQLIGAGCVARIRAAWVGNVSAGLGHNYRRAAERGTPRPIEVED
                     HSNLSIALGLHAAALGLPFLPTRSLLGSDIAAGSDAFADLRDPWSEDRLLAVRAIRPD
                     VAIVGAQRADENGNAHAWGNLGVTQDAVMAADRVILLAEEIVTGDVIRSDPGRVLVPG
                     FRVSAIVHEPGACHPSPVQGCYRRDHAAFAEYHEATRDEEGFEAWLREWILEVPDRAA
                     YLERLGPRWDALRDLHEARAAVTDYGW"
     gene            1578..2339
                     /locus_tag="VF097_07385"
     CDS             1578..2339
                     /locus_tag="VF097_07385"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003320712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX6262652.1"
                     /translation="MTVDLAIGELMVAAAAREIRDQERIFVGMRLPMLAFFLARRTHA
                     PAAVGIFENGVIRDRPSPAPLATMSDTPNVAGALWCGGMLQPMGLLQRGEVDVGFIGG
                     AEVDRHGNVNSSYIGDPKAPKVKLPGSGGGADIASLARRILLIMPHDRRRLTEHVSYV
                     TSPGHGNGPGWRDRVGLPPGGPSALITTMAVFRFVDGEALLESWHPGFDEGDVLRATG
                     WRVRPAEDAGPTPPPSEEELAVIRELDPDGVWTRR"
     gene            complement(2336..2920)
                     /locus_tag="VF097_07390"
     CDS             complement(2336..2920)
                     /locus_tag="VF097_07390"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX6262653.1"
                     /translation="MGRVAAVVLAAGSSSRMGRPKQLLEVGDAPMLQHAVDAAASADV
                     DEVIVVLGHAAAAVEEALRLPGGARVVRNPDHGEGLSTSLATGLRALGGEHAAAVVIL
                     GDQPEIEASSIRAVVGAYRETGARVVQASYRGTADHPVLLDRRAWPEVLAVTGDRGAR
                     EVIASHPRWVHRVELDLDPPRDVDTPADYEGLRR"
     gene            complement(2922..3932)
                     /gene="moaA"
                     /locus_tag="VF097_07395"
     CDS             complement(2922..3932)
                     /gene="moaA"
                     /locus_tag="VF097_07395"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013289331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX6262654.1"
                     /translation="MKSRAAPRSGPLVDRFGRTVSDLRVSVTDRCNFRCTYCMPAEGL
                     PWLPSEDLLDTGEIRRVIGLFVRLGVREVKVTGGEPTVRPDLPEVVASIREAGPSLDL
                     SMTTNGLLLDRLAGPLREAGLDRVTVSCDSLLRHRFAEMTRRDALDRVLSGLRAAEEA
                     GLAPVKVNCVVLRGENDVEALDFARMARRTGHVIRFIEYMPLDAQEAWERERVVPSAE
                     LHDRIHAMFPLVRDGDGEGPAAVFRFADGAPGGLGFISSVTEPFCETCNRVRLTADGQ
                     LRACLFSLDEKDLRGVLRGGGSDADLERVVREAVWGKWAGHRINHPDFVRPGRSMSMI
                     GG"
     gene            complement(3932..5065)
                     /locus_tag="VF097_07400"
     CDS             complement(3932..5065)
                     /locus_tag="VF097_07400"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX6262655.1"
                     /translation="MRSEIGEVLAAIERLRERGERSALATVVAVRGSTYRGPGARVLV
                     AEGGDLTGTISGGCLEGDVADVARVVMEDEEPRLVDFDLTADDEAVWGWGLGCNGALE
                     VFVEPVEESLGLLEPLRQALEEDRPVALATVVESGVDGVEPGARLLVHPDGRVEGDVG
                     RPEVDEVLTVAGRDALERGRSTTERLSTAGGDVRAFVEVLEPASRLLVCGAGHDAIPL
                     VRIAASLGWRPFVVDDREDLLTPDRFPHAAGFVPVGRPEEAARAAGVDPRTSVVIMSH
                     NYLRDLGYLRSFLGSGAAYLGCLGPRTRLERLLGDLEREGIVPSEEDRAALRGPAGLD
                     LGADGPDEIALAIVSEILASRSGRDGGPLRARGGPIHEREGAG"
     gene            complement(5062..5523)
                     /gene="moaC"
                     /locus_tag="VF097_07405"
     CDS             complement(5062..5523)
                     /gene="moaC"
                     /locus_tag="VF097_07405"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011646086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX6262656.1"
                     /translation="MAQPDDAARMVEVGGKEITAREAVAEARVTMTPEACRRLLVGTP
                     KGDPIEAARVAGLMAAKRTPDLLPFCHPVALTGAEVSITSDAQAGVLRVRASVRAADR
                     TGVEMEALTAVSVAALSLYDTAKAYDRGVVIGEIRLLEKRGGRSGDHGSPG"
     gene            complement(5523..>6574)
                     /gene="glp"
                     /locus_tag="VF097_07410"
     CDS             complement(5523..>6574)
                     /gene="glp"
                     /locus_tag="VF097_07410"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX6262657.1"
                     /translation="RAADVGGAHRERPVGLRPAGEVRMGSGDRLALSEGEAIAIPTGG
                     AVPEGADAVVPIELCSTDGERVWVLQPAGPGEHVRPAGEDAEAGDVLVPAGRRLLGPD
                     LGLLASSGTASAPVHPRVRVGVVSTGDELVPVGRPLAPGRIHDANAFTLIAAVEEAGG
                     VAVHPPIVPDDPDRLVEALDRLAEEVDVLISSGGVAVGERDPVKRAFAGRGEVELYEV
                     AMQPGKPQAFGSWRNRPFFGLPGNAVSVFVSFEVFVRPALMRMMGRAPFRPEAVAVLE
                     EDVAGLPRKTRFARVRLRRDGARLLAAPTGSHRSSLLTPLAGAHGLAVIPAGVEAVRA
                     GHPCPVIVFRDPEEG"
BASE COUNT          904 a         2505 c         2370 g          795 t
ORIGIN      
        1 gatcgggcac gccgggcgca ggggctccac ccgccctcgc catcgggggc tggaccttcc
       61 gctgggcgcc tccggctggc ccctgctctc ggcttccccg ctgccgtaca caagcgtctc
      121 ccccgcgcct cgggagatgg atcgggccga catggatcgg atccgagacg cgttcgtccg
      181 cgccgcgcgg gccgcaacgg acgcgggcgc cgacgtgctc gggatccacc tcggccacgg
      241 ctacctgctg gcgagcttcc tctccccact gacgaacgtc cgtgaggacg ggtacggggg
      301 ctcgctcgag gggcgagccc gcttcccgct ggaggtggtg gaggccgtcc gggcggcgtg
      361 gcccgagaac cgccccctct ggggcacggt cacggtggac gactggaagc ggggcgggct
      421 gtcgccgatg gaggcgatcg agctgtgcgc catgctccgg gaccggggat gcgacgtcct
      481 ggagcccgtg gccgggcaga ccacgccgga ctcccggccg accttcggtc ggatgttcct
      541 ggcgccctac gcggaccgag tccggaacga ggtcggggtg gcgacgatga cctccggcgg
      601 catcacgacc accggccagg tgaacacgct cgtagccggg ggccgggcgg acctctgcgt
      661 gatggacccg tgagcaagct tgccagcatg cgggatgccg tgtccgagca cgtctcggac
      721 ggctcgagcg tcgtcatggg gacctgtctc gagagcatga tcccgttcgc ggccggacac
      781 gagctgatcc ggcaggagcg acgcgagctc acgctgatcg ggccgatctc cgacatccta
      841 ttcgaccagc tgatcggcgc gggctgcgtg gcgaggatcc gcgcggcctg ggtggggaac
      901 gtcagcgcgg ggctcggcca caactaccgg cgcgcggccg aacggggcac gcctcgcccc
      961 atcgaggtcg aggaccactc caacctctcg atcgcgctcg gactccacgc ggcagcgctg
     1021 gggctcccct tcctgcccac gcggtcgctg ctgggaagcg acatcgcggc ggggagcgac
     1081 gccttcgccg acctccgcga tccctggagc gaggaccgcc tgctcgcggt ccgggcgatc
     1141 cggccagacg tcgccatcgt cggcgcccag cgtgccgacg agaacggcaa cgcccatgca
     1201 tgggggaacc tcggcgtgac gcaggatgcg gtcatggcgg cggatcgcgt gatcctgctg
     1261 gcggaggaga tcgtgaccgg ggacgtgatc cggagcgacc cgggccgcgt gctcgtgccc
     1321 gggttccggg tctcggccat cgtgcacgag ccgggcgcct gccacccctc gcccgtgcag
     1381 ggctgctacc ggcgggacca tgccgccttt gccgagtacc acgaggccac gagagacgag
     1441 gaggggttcg aggcgtggct gcgggagtgg atcctcgagg tcccggaccg cgcggcctac
     1501 ctggagcggc tggggcccag atgggacgcg ctacgggacc tgcacgaggc gcgcgccgcg
     1561 gtcacggact acggctggtg acggtggacc tggcgatcgg cgagctcatg gtcgcggccg
     1621 cggcgcgcga gatccgcgac caggagcgga tcttcgtggg gatgcgcctc cccatgctgg
     1681 cgttcttcct cgcccgccgg acgcacgccc ccgccgccgt gggcatcttc gagaacggcg
     1741 tcatccgtga ccgccctagc cccgcgccgc tcgccacgat gtccgacacc cccaacgtcg
     1801 ccggcgccct gtggtgcggc ggcatgctcc agccgatggg gctgctccag cggggcgagg
     1861 tcgacgtcgg gttcatcggc ggcgccgagg tcgaccgcca cggcaacgtc aacagctcct
     1921 acatagggga cccgaaggcc ccgaaggtga agctgcccgg gagcggtggc ggggcagaca
     1981 tcgcctcgct ggcgcggcgg atcctcctca tcatgccgca cgaccggcgc cggctcaccg
     2041 agcacgtgag ctatgtcacc tcgccgggcc atggaaacgg ccccgggtgg cgggatcggg
     2101 tcgggctccc gcccggcggg ccctcggcgc tcatcacgac gatggccgtg ttccggttcg
     2161 tggacggcga ggcgctgctg gagagctggc atccgggctt cgacgagggc gacgtgctcc
     2221 gggcgaccgg ctggcgggtg cgcccggccg aggacgcggg ccccacgccg cccccctccg
     2281 aggaggagct tgcggtgatc agggagctcg accccgacgg cgtctggacc cgtcgctagc
     2341 gccggagacc ctcgtagtcc gcgggagtgt ccacgtccct cggcggatcg agatcgagct
     2401 ccacgcgatg gacccaccgg ggatgggagg cgatcacctc gcgggcccca cggtcccccg
     2461 tcacggcgag gacctcgggc caggcccggc gatcgagcag gacggggtgg tcggcggtcc
     2521 ctcggtacga ggcctgcacc actcgggcgc cggtctcccg gtacgcgccg acgacggcgc
     2581 ggatcgacga cgcctcgatc tcgggctggt ccccgaggat caccaccgcg gcggcgtgct
     2641 ccccgcccag cgcccggagc ccggttgcga gcgaggtgga gagcccctcg ccgtgatcgg
     2701 ggttccgaac gacgcgcgcg ccgccgggga gccggagcgc ctcctcgacc gcagccgccg
     2761 cgtggccgag gacgacgatc acctcgtcga cgtcggcgga cgcggcggcg tccaccgcgt
     2821 gctgcagcat cggggcgtcc ccgacctcga ggagctgctt cggcctcccc atccgcgagg
     2881 agctccccgc cgccagcacg acggcggcca cacggcccac ctcagccccc gatcatcgac
     2941 atgctgcggc cgggccgtac gaagtcgggg tggttgatgc ggtggcccgc ccacttcccc
     3001 cagaccgcct cgcggacgac gcgctccaga tcggcgtcgc tgccccctcc gcggaggacc
     3061 ccccgcaggt ccttctcgtc gagggagaag aggcacgctc ggagctgccc gtcggccgtc
     3121 agccgaaccc tgttgcacgt ctcgcagaac ggctccgtca ccgaggagat gaagcccagg
     3181 ccgccgggag ccccatcggc gaaccggaag acggcggccg gaccctcccc gtcgccgtcc
     3241 cgcaccaagg ggaacatggc gtggatccgg tcgtggagct ccgccgaggg cacgacccgc
     3301 tcccgctccc aggcttcctg agcgtcgagc ggcatgtact cgatgaaccg gatcacgtgg
     3361 ccggtgcgac gggccatccg ggcgaagtcc agcgcctcga cgtcgttctc gccccggagc
     3421 acgacgcagt tgaccttgac cggggccagc ccggcctcct cggcggcccg cagcccggaa
     3481 agcacgcggt cgagggcgtc gcggcgcgtc atctcggcga agcggtgccg cagcagcgag
     3541 tcgcacgaca ccgtgacccg gtccaggcct gcctctcgga gcggtccggc cagccggtcg
     3601 agcaggaggc cgttcgtcgt catggagagg tcgagcgagg gaccggcctc gcggatcgag
     3661 gccacgactt caggcaggtc ggggcggacg gtgggctccc cgcccgtgac cttcacctcg
     3721 cgcaccccga ggcgcacgaa cagtccgatc acccggcgga tctcacccgt gtcgagcagg
     3781 tcctcgctgg gcaaccaggg caggccctcg gccggcatgc agtacgtgca ccggaagttg
     3841 cagcgatcgg tgaccgagac gcggaggtcc gagacggtcc gcccgaatcg gtccaccagc
     3901 ggaccgctcc gcggcgcggc acggctcttc atcaccccgc cccctctcgc tcgtggatcg
     3961 gccccccgcg ggcgcggagg gggccaccat cgcgccccga ccggctggcc aggatctcgg
     4021 agacgatggc gagagcgatc tcgtcgggcc cgtcggcgcc gaggtcgagc cccgccgggc
     4081 cgcgcagcgc ggcccgatcc tcctcggacg ggacgatccc ctcgcgctcc agatcgccca
     4141 gaagccgctc cagccgagtg cgggggccga gacagccgag gtacgccgcg cccgagccga
     4201 ggaacgaccg gaggtagccc agatcccgca ggtagttgtg gctcatgatc acgaccgagg
     4261 tccggggatc gacgccggcc gcccgggccg cctcctcggg ccgccccacc gggacgaagc
     4321 cggcggcgtg ggggaagcgg tctggggtga gtaggtcctc acggtcgtcc acgacgaacg
     4381 ggcgccatcc gagcgaggcg gcgatccgga cgagagggat cgcgtcgtgc cccgccccgc
     4441 agaccaggag ccgggaggcg ggctcgagca cctccacgaa ggcccgcacg tccccgcccg
     4501 ccgtggacag acgctcagtc gtcgagcggc cgcgctccag ggcatcccgc cccgccacgg
     4561 tcaggacctc gtcgacctcc ggccgtccga cgtcgccctc gacgcgaccg tccgggtgga
     4621 ccagcagccg cgcgccgggc tcgacgccgt cgacgcccga ctccaccacg gtcgccaggg
     4681 cgaccggccg gtcctcctcc agtgcctgac ggaggggctc caggagcccc aggctctcct
     4741 ccaccggctc gacgaacacc tccagggccc cgttgcatcc gagaccccat ccccacaccg
     4801 cctcgtcgtc ggcggtcaga tcgaagtcga cgaggcgggg ctcctcgtcc tccatcacga
     4861 cccgggccac gtcggccacg tccccctcga ggcatccccc gctgatcgtc ccggtgaggt
     4921 cgccgccctc ggccaccagc acgcgcgcgc ccgggccccg gtaggtcgac ccgcgcacgg
     4981 ccacgaccgt ggccagggcc gaccgctcgc cccgctcccg taggcgctcg atcgccgcca
     5041 gcacctcgcc gatctcgctc ctcatcccgg gctcccgtgg tctccgctcc gcccgccccg
     5101 cttctcgagg aggcggatct ccccgatcac gacgccgcgg tcgtaggcct tggcggtgtc
     5161 gtagagcgag agcgccgcca ccgacacggc cgtgagcgcc tccatctcca cgcccgtgcg
     5221 gtcggcggcc cgcaccgaag cccgaacccg gaggaccccg gcctgggcgt cagaggtgat
     5281 cgagacctcg gccccggtca gcgccacggg atggcagaac gggaggaggt cgggcgtccg
     5341 cttggcggcc atcaggccgg cgacgcgggc cgcctcgatc ggatcgccct tgggcgtgcc
     5401 gaccagcagg cggcggcacg cctcgggcgt catcgtcacc ctggcctccg ccacggcctc
     5461 ccgggccgtg atctccttgc cgccgacctc gaccatgcgc gcggcgtcat cgggttgggc
     5521 catcagccct cctccgggtc gcggaagacg atgaccgggc aggggtggcc ggcgcgcacc
     5581 gcctcgacgc ccgcggggat gacggcgagc ccgtgcgcgc cggccagcgg ggtcagcagg
     5641 ctcgagcggt ggctgccggt gggggcggcc agcaggcgag cgccgtctcg gcgcaggcgc
     5701 acccgcgcga acctcgtctt gcgcgggagg ccggccacgt cctcctccag gacggcgacg
     5761 gcctcgggac ggaagggcgc ccgtcccatc atccgcatca gcgcggggcg cacgaagacc
     5821 tcgaacgaca cgaagaccga gaccgcgttg ccggggagac cgaagaaggg acggttccgc
     5881 caggacccga acgcctgggg cttccccggc tgcatcgcga cctcgtagag ctcgacctcc
     5941 ccccggccgg cgaacgcccg cttcacgggg tcgcgctccc cgacggccac gccgccggac
     6001 gagatcagca cgtccacctc ctcggccagg cgatccaggg cctcgaccag ccggtcgggg
     6061 tcgtcgggga cgatcggcgg gtggaccgcc accccgccgg cctcctccac cgcagcgatg
     6121 agcgtgaacg cattggcgtc gtggatccgc cccggagcga gcggccgccc gaccggcacg
     6181 agctcgtccc ccgtcgagac gacccccacc cggacgcggg gatggacggg agccgacgcc
     6241 gtccccgagg aggcgagcag cccgaggtcg gggccgagga gccgcctccc cgccgggacc
     6301 aggacgtcgc cggcctcggc gtcctcaccc gccggccgga cgtgctcccc cggacccgcc
     6361 ggctgaagca cccagacccg ctcgccgtcc gtggagcaga gctcgatcgg gacgacggcg
     6421 tcggccccct cggggacggc cccgcccgtg gggatggcga tcgcctcgcc ctcggaaagg
     6481 gcgaggcgat cgccggaccc catccggacc tcgccggccg ggcgcagccc gaccgggcgt
     6541 tcgcggtgcg cgccgccgac gtcggcggcg cgca
//
LOCUS       DASUTZ010000169        11717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1605702, whole genome shotgun sequence.
ACCESSION   DASUTZ010000169 DASUTZ010000000
VERSION     DASUTZ010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11717
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1605702"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /locus_tag="VF097_07415"
     CDS             <1..1097
                     /locus_tag="VF097_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015224672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6262658.1"
                     /translation="PLFEFSGACAGCGETPYLGLLSRLFGDRTLVANATGCSSIFGGN
                     LPTTPWSKNDEGRGPAWANSLFEDNAEFGLGMRAGLDRRAERARRLLAGLSWELGWEL
                     PQAILRADQDSEEEIARQRARVAELKERLEAVDSPEARELLGLADDLVRKNVWIVGGD
                     GWAYDIGFGGLDHVLASGRDVNVLVLDTEVYSNTGGQASKATPRAAAAKFASAGKGTA
                     KKDLGMIAMTYGDVYVAQVALGADNPQTVKALAEAEAYPGPSLVIAYGTCIEHGIEMA
                     TSMTHQKEAVAVGHWPLYRYDPRRAREGSPAFQLDSRRKEGAFRDFAMKETRFAALAR
                     SNPLRAEALLEAAEEDIADRWRHYERLAAG"
     gene            complement(1192..1539)
                     /locus_tag="VF097_07420"
     CDS             complement(1192..1539)
                     /locus_tag="VF097_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262659.1"
                     /translation="MTNAIAVYAAAVGVLMAAWWALALLRGAWGRKDRTYAELGLHLG
                     AELLTAAALLASGIAVLAGGPTAFLAAALGMLLYTTIASPGYFLARGEVPMVAMFGVL
                     AALTASGLVVLLV"
     gene            complement(1541..1816)
                     /locus_tag="VF097_07425"
     CDS             complement(1541..1816)
                     /locus_tag="VF097_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015747713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HEX6262660.1"
                     /translation="MVETREDEEVLREQAVERLKRKRDFTAHLLAYVMVNTVLIVVWA
                     MTGAAFFWPIFPLAGWGVGLGFHAWDTFSRPPSEERIREEMDHLRAA"
     gene            complement(1832..2545)
                     /locus_tag="VF097_07430"
     CDS             complement(1832..2545)
                     /locus_tag="VF097_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262661.1"
                     /translation="MVEAGTPVAWGEGLVWLVGVIVGGFLISWVLTDVLRMHRALYVA
                     LLTLLAWGLTIGYVMWSGSGPAFWIERWPWGMLGALVAGAFLAVMVSRRPAEREADGM
                     GPGDALWEGVVYGSAEGLLLSVLPVLITWQLLGALGWGDAWRALAALAASVVVIVAHH
                     LGYPEYRGRAIASPVIGCTVLSIAYLATGSPIAAIGGHIVLHLAMRMRGMELPPHAEA
                     IPGGERTRAGGELAAARSG"
     gene            2673..3002
                     /locus_tag="VF097_07435"
     CDS             2673..3002
                     /locus_tag="VF097_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262662.1"
                     /translation="MDPREERVARNESTSREINEGIQEAHEDAGPAEQVRMLCECGHR
                     TCERVIAITLPEYERVRSDGRRFAVVHEHVIPDVEDVVEENERFVVVVKREGTPADVA
                     LEEDPRG"
     gene            3047..4435
                     /locus_tag="VF097_07440"
     CDS             3047..4435
                     /locus_tag="VF097_07440"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX6262663.1"
                     /translation="MEVRADSPPRVLADRYEVGELLGAGGMASVYRGHDRILQRPVAV
                     KILDASLAVDDRFLRSFVREARAAAGLIHPNVVTVFDTGVDDDQHFILMELVEGSTLQ
                     QVLAREHRLEAERARRIAIDVARALTAAHGRGIVHRDVKPANVLMGEDGRPRVADFGI
                     ARPTDQDDESRVVFGTAAYLSPEQARGRPTTPASDVYSLGCVLYEMLTGRRPFTGDNP
                     VSVAVKHLRYTAPPVEALAPDVPPALAAVVYRAMQKDPRRRFPDAGAMLAALEGAATV
                     SGLPERRRPGFLRWAAASVSLAFIAVLAPALIGAGGDAPEPGARVIAEERGRSPARVD
                     VPAVTGMSRQEAGRALRRADLIPIFRGAPLHQSASHVVATQDPQAGATLAVGSFVTLE
                     LVPADADVAPPAGNGPSGKRQGPSGSGKSAEGRSDETRPDASPSPSLSPTPSPSPTPT
                     PTPSPSPTEGVR"
     gene            4441..5811
                     /locus_tag="VF097_07445"
     CDS             4441..5811
                     /locus_tag="VF097_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6262664.1"
                     /translation="MFLERFHEEKLGHASYLVGSQDAGVALLVDPRRDVDPYLEAADR
                     RGVRIAHALDTHGHNDYLSGLSEIAARTDAEILASAHGEARYPHRPLKDGQSLEVGEA
                     VVEAMHTPGHTPEHLALLVRDASVDDLEPLMLLSGGSLLVGDVARPDLLGGRDEAREA
                     ARVLCRTLAERILTLPDHVEVHPTHVSGSLCAGSIGSRLTTTIGYERRTNPSLARPED
                     CVSLEDLPAVPPYWRRMRSQNLEGVALLGTVTPPPALQPEEVERRLAEGALALDTRTP
                     EDFAGGHVPGALSAGLGGSFPTWAGTVLPEGARVVLVLDDPGSAMEAAWHLLRIGYEP
                     PLGFLEGGMQAWRADGKPVREMPVMDVRELRRRLDDVAVLDVRQPGEWRDGHIAGATH
                     ITGAELERRLDEVPSRRPLAVLCGTGYRSSASASLLLRKGFDDVWNVLGGSTAWDAAG
                     YPTVRA"
     gene            5808..6227
                     /locus_tag="VF097_07450"
     CDS             5808..6227
                     /locus_tag="VF097_07450"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX6262665.1"
                     /translation="MSDPACIFCRIVSRDALALTIYEDDATMAFMDINPATDGHCLVI
                     PKRHAPDVWTLPEEDGVAVWRSVRRLAGAVRDALRADGLNILQSNGRAALQTVFHYHV
                     HVIPRYVGDGIHVPLMRAPGDRSRLDEFADLIRAQLT"
     gene            complement(6224..7093)
                     /locus_tag="VF097_07455"
     CDS             complement(6224..7093)
                     /locus_tag="VF097_07455"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6262666.1"
                     /translation="MVVLAFCGLGQMGAPMARRLLQAGHEVAVWNRTEEKARPLVEAG
                     ARAAASPADAAEGAEAAFTMLADPGADREVVLGTGGLAEGLREDATLIEMSTIGVRAV
                     REIAEALPEGLGVLDAPVLGSVTQAEEGTLRIFVGGPSDLVDRWAPTLRAMGTPRRFG
                     ELGAGAAMKLVVNSTLGALMAGLGEALALADALGVDEEAALDVLEGSPIGATTRSKRD
                     KIVSGRYPPNFRLGLAAKDLRLVLDEAAEAGARLPVAEAVLAGLEAARDAGLGGLDYS
                     AVIPYVRGRPATG"
     gene            7135..7482
                     /locus_tag="VF097_07460"
     CDS             7135..7482
                     /locus_tag="VF097_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262667.1"
                     /translation="MSDEYAPLPRVHPVMAAMAGGALWGAMGYAVLWGLSPIVVGRAF
                     VVSAIGTILFLPVRLVLWVLRWVEGLVGRPFEFAHSNWWIGALAAVVGAGIGIALTLT
                     ARTAFRRLRPTPD"
     gene            7559..8458
                     /locus_tag="VF097_07465"
     CDS             7559..8458
                     /locus_tag="VF097_07465"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX6262668.1"
                     /translation="MAGSPPTPRGWRALPTLAVRAVRDSFEDRIFGLGAEVAFFFLLS
                     FPPALLTILGTLGYVSQVLGEDVAAAMRDQILSVAGSFLTEETVTETVRPLVNRLLAE
                     GRADVASFGIVLTLWAASRATTRLIEAVAIAYDEEDERSAVRRRLLSLGMTVGTIVAL
                     VLVIPILVAGPRLLEALAEPLGLTAAVAAGWRVLYWPVVALIGIGLLSLFYHLASPLE
                     TRWRRDVPGAFAAAGVWLLGGLGLRVYLGFSLDSGAYGPLAAPIAVLLWMYVTALAVL
                     LGAEVNAEIERMWPDRPRKKRDA"
     gene            complement(8455..8841)
                     /locus_tag="VF097_07470"
     CDS             complement(8455..8841)
                     /locus_tag="VF097_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262669.1"
                     /translation="MAGRGGDRLRAAALGAIALGLAPLGACDDAPGDAGPQRLRVEMR
                     EFAFDPSEIALRAGVPVDVRLRNVGRVEHDWTLLAPGGEEVSYVYVQPGRRGRDRFTL
                     DEGTYDLLCTIEGHAESGMVGSVVAG"
     gene            complement(8814..9077)
                     /locus_tag="VF097_07475"
     CDS             complement(8814..9077)
                     /locus_tag="VF097_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262670.1"
                     /translation="MAGWLEFRQCPGCGFDPATAEGEKGCHYVDCPYLPAELDVLCPQ
                     CRFDFYTGEGNPSCDDPSTCAEGATARANVENVRRWRAGAAIG"
     gene            complement(9163..10344)
                     /locus_tag="VF097_07480"
     CDS             complement(9163..10344)
                     /locus_tag="VF097_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262671.1"
                     /translation="MRFSGPSAAAQVLARIWLLAAAASLLLPPADRLGWWLPIHLALA
                     GAAGSAVSGTLPLFANALAGAHDLPQRWDPLLLVTAGAASIAIGRPLGITWLLGVGGG
                     AYALGSLRLTWLVLTARRAGSNRRHAAALFAYAGAALSLTLGAVLGALLGAGTVGGAD
                     VLDIRAAHVTLNAEGFLALAVAGSLLVLVPITLRVRAPDATGRLVVGGLGLGVILQAA
                     GHVASWTPLVAVGAGAALLGAADLVHVAAGSVARRPRMPEPGAALHLLSVPLWLVGVT
                     AAALILALTGNAGWPLSLVAALTLGVFIQALLGAWSYLLPVSDPAGSEARRARLERTR
                     RWAWPRAAAFNAGAALVALAAAGWVPSPLGTIGLVGAAVAALAAIVLASLGAPVRGEA
                     P"
     gene            complement(10348..10860)
                     /locus_tag="VF097_07485"
     CDS             complement(10348..10860)
                     /locus_tag="VF097_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010593700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEX6262672.1"
                     /translation="MAELAELPVPEEAEEKKLVIMAWSGDLDKVWPTLILATSGAAMG
                     MRTTVFFTFWGLFPLVKNDVRITGENWMQKMLSMMNRGGTEHLKLGKMNFAGMGPWMM
                     KKLAAEHKVASPQELLELARDMGVRLVPCQMTMDMLGLRREDLIDGIDEPAGATTALL
                     EAQDAVTLFI"
     gene            complement(10866..11096)
                     /locus_tag="VF097_07490"
     CDS             complement(10866..11096)
                     /locus_tag="VF097_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011067043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX6262673.1"
                     /translation="MKTIAKTLDLKGLSCPLPVVKTAQAMKEAAAGDLIEVLATDPGS
                     VPDFKAWAGATGNELVEQTEQDGVYRFLIRKG"
     gene            complement(11093..>11717)
                     /locus_tag="VF097_07495"
     CDS             complement(11093..>11717)
                     /locus_tag="VF097_07495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEX6262674.1"
                     /translation="SPLNRAFTIESASKLVQPIMERRGIGLETFFNVEAVDPAAHVVE
                     SLEGEKAEYDLLVLVPPHRGQEVVSASGLGDAGGWMPTDPATLQHKEHERIFAIGDAT
                     NLPISKSGSTAHFEGPVVASRIASLIRGTAPKASYGGRVMCFLETGGGRSTVLRFDYE
                     HPPVPPKPSRRWHAAKWLFNRLYWNTVPQGRIPEFLGSPGSEEGSEA"
BASE COUNT         1622 a         4334 c         4196 g         1565 t
ORIGIN      
        1 agccgctgtt cgagttctcc ggcgcctgcg ccggctgcgg ggagaccccg tatctcggcc
       61 tgctgagcag gctcttcggc gaccgaacgc tggtggccaa cgcgaccggg tgctcctcga
      121 tcttcggggg gaacctcccc accaccccct ggtcgaagaa cgacgaggga cggggccctg
      181 cctgggccaa ctcactgttc gaggacaacg ccgagttcgg cctggggatg cgcgccgggc
      241 tcgaccgccg ggcggagcgg gcccggcggc tgctggcggg gctgtcgtgg gagctgggat
      301 gggagctgcc ccaggcgatc ctccgcgccg accaggactc cgaggaggag atcgcccgcc
      361 agcgcgctcg tgtcgcggag ctgaaggaac ggctggaggc cgtggactcg ccggaggcca
      421 gggagctgct ggggctggcc gacgacctcg tccgcaagaa cgtgtggatc gtcggcggcg
      481 acggatgggc ctacgacatc gggttcggcg ggctggacca cgtcctggcg tcgggtcggg
      541 acgtgaacgt cctcgtcctc gacaccgagg tctactcgaa caccggcgga caggcctcga
      601 aggccacgcc gcgggcggcg gcggcgaagt tcgcctcggc gggcaagggg acggccaaga
      661 aggacctcgg gatgatcgcg atgacctacg gcgacgtgta cgtggcccag gtggcgctgg
      721 gcgccgacaa cccgcagacc gtgaaggcgc tggccgaggc cgaggcgtac cccggtccct
      781 cgctggtcat cgcctacggc acgtgcatcg agcacggcat cgagatggcc accagcatga
      841 cccaccagaa ggaggccgtg gccgtcggtc actggccgct gtaccggtac gatcccaggc
      901 gcgcccgcga gggatcgccg gcgttccagc tcgattcccg gcggaaggaa ggcgcgttcc
      961 gcgacttcgc gatgaaggag acgcggttcg cggcgctcgc acggtcgaac cccctcaggg
     1021 ccgaggccct gctggaggcc gcggaggagg acatcgccga ccggtggcga cactacgagc
     1081 ggctcgccgc cggatgatcc tccgaagaag gcctccgcat cgaccacttc cgcgctgagc
     1141 ccggcgaacc ggttcgccca agcgctcgcc ctcatcaagc cgaagacggc cctagaccag
     1201 caggaccacc agcccggacg cggtgagcgc cgcgaggacg ccgaacatgg ccaccatggg
     1261 gacctcgccg cgagccagga agtacccggg gctggcgatc gtcgtgtaga ggagcatccc
     1321 cagcgctgcg gcgaggaacg ccgtcggccc tcccgccagg acggcgatgc cggaggcgag
     1381 gagggccgcg gccgtcagga gctccgcgcc gaggtgcagc cccagctcgg cgtaggtgcg
     1441 atcctttcga ccccacgcgc ctcgcagcaa cgccagggcc caccaggcgg ccatcagcac
     1501 tcccaccgcc gccgcgtaga cggcgatcgc gttcgtcacg ctacgcggcc cggaggtggt
     1561 ccatctcctc ccggatccgc tcctcgctgg gagggcggct gaacgtgtcc caggcgtgga
     1621 acccgagccc gaccccccat ccggccaggg ggaagatggg ccagaagaac gcggcccccg
     1681 tcatcgccca gaccacgatg agcacggtgt tcaccatcac ataggcgagc aggtgagcgg
     1741 tgaagtcccg cttcctcttc agtctctcca ccgcctgttc ccgcaggacc tcctcgtcct
     1801 ctctcgtctc gaccatcgct ccctcctcct ttcagccgga cctcgccgcc gccagctccc
     1861 ctccggcgcg ggtgcgctct ccgccgggga tggcctcggc gtgcggcgga agctccatac
     1921 ctctcatccg catggcgagg tgcagcacga tgtggccgcc gatcgccgcg atcgggctgc
     1981 cggtcgccag gtaggcgatg ctcagcaccg tgcacccgat gaccggagag gcgatcgctc
     2041 gcccccggta ctcggggtag ccgaggtgat gggcgacgat gaccacgacg ctcgcggcga
     2101 gggccgcgag ggccctccag gcgtcgcccc aacccagcgc cccgagcagc tgccaggtga
     2161 tcaggacggg cagcacggag agcagcaggc cctccgccga cccgtagacg accccctccc
     2221 acagcgcgtc gccggggccc atcccgtcgg cctcacgctc ggcgggtcgg cgggagacca
     2281 tcaccgccag gaacgctccg gccaccagag cgcccagcat cccccaaggc caccgctcga
     2341 tccagaacgc ggggccgctc ccgctccaca tgacgtaccc gatcgtgagg ccccacgcga
     2401 gcagcgtgag cagcgccacg tagagcgccc gatgcatacg cagcacgtcg gtgaggaccc
     2461 acgagatcag gaagccgcca acgatcacgc cgaccagcca caccaggccc tcaccccacg
     2521 cgacgggtgt gccagcttcg accatgccct tccacctcct ccggtcccac gctaggggct
     2581 ccgcgcgctc cctccgaggg ccgggggtcc cggcgggtgg gtgaccttcg ggctcgctcc
     2641 agggatcctc gccactcggt acgatgcagg acatggatcc cagggaagag cgggtcgcgc
     2701 gcaacgagtc gacctcgcgg gagatcaacg agggcatcca ggaggcccac gaggacgcgg
     2761 ggcctgccga gcaggtccgg atgctgtgcg agtgcggcca tcgaacgtgc gagcgggtga
     2821 tcgcgatcac ccttcccgaa tacgagcggg tccggagcga cgggcgccgc ttcgccgtgg
     2881 tccacgagca cgtgatcccc gacgtcgagg acgtcgtgga ggagaacgag cgcttcgtgg
     2941 tggtcgtgaa gcgcgagggg acgcccgccg acgtcgcgct cgaggaggac ccccggggct
     3001 agcacaggcg tttcctgcgc ggcgccgtcg ggtatgcggc catgagatgg aggtgcgcgc
     3061 agactcgccg ccacgcgtgc tcgccgaccg ctacgaggtc ggcgagcttc tcggcgcggg
     3121 ggggatggcg agcgtgtacc ggggccacga ccggatcctc cagcgtcccg tggccgtcaa
     3181 gatcctcgat gcctcgctcg cggtagacga ccggttcctg cgcagcttcg tccgtgaggc
     3241 tcgcgcggcc gcgggcctga tccaccccaa cgtggtgacg gtgttcgaca ccggggtgga
     3301 cgacgatcag cacttcatcc tgatggagct ggtcgaaggg agcaccctcc agcaggtcct
     3361 ggcccgggag catcgcctgg aggcggagcg ggcccgccgg atcgcgatcg acgtggccag
     3421 ggccctgacg gcagcccacg gacgagggat cgtccaccgg gacgtgaagc cggccaacgt
     3481 gctcatgggc gaggacgggc gtcctcgcgt ggcggacttc gggatcgctc gccccaccga
     3541 ccaggacgac gagagccgcg tcgtcttcgg gaccgcggcc tacctctcgc ccgagcaggc
     3601 aaggggccgt cccaccacgc ccgccagcga cgtgtactcg ctcggctgtg tcctctacga
     3661 gatgctcacc ggacgtcgcc cgttcaccgg agacaacccc gtgtcggtgg cggtgaagca
     3721 cctccgatac actgcgccgc cggtggaggc cctggccccc gacgtgcctc cggcgctggc
     3781 ggcggtcgtc tacagggcca tgcagaagga tcctcgccgc cgcttccccg acgcgggagc
     3841 catgctcgcc gcgctggagg gcgccgccac ggtctcgggg ctccctgagc ggcgccgccc
     3901 ggggttccta cggtgggcgg ccgcctcggt gagcctggct ttcatcgccg tcctggcgcc
     3961 ggcgctgatc ggggcgggcg gcgacgcccc cgagcccgga gcgcgcgtga tcgccgaaga
     4021 gcgcggacgg agcccggccc gtgtggacgt cccggccgtc accggcatga gtcggcagga
     4081 ggccggccgg gccctccgcc gggcggacct gatcccgatc ttccgcgggg caccgctgca
     4141 ccagagcgcg tcccacgtcg tggccaccca ggacccgcag gcgggtgcca ccctcgccgt
     4201 ggggtccttc gtcaccctgg agctcgtgcc ggcggacgcc gacgtcgccc cgccggcggg
     4261 gaacggcccg agcgggaagc ggcaaggacc gtccggctcg gggaagtcgg ccgaggggcg
     4321 ctcggacgag acccggcccg acgcctctcc gtcgccgtct ctctcgccca cccccagccc
     4381 gagcccgacg cctacgccga cgccgtctcc cagcccgacc gagggcgtac gctaggccga
     4441 atgttcctcg aacggttcca tgaggagaag ctcgggcacg cctcgtacct cgtcgggtcg
     4501 caggacgcgg gcgtggccct gctggtcgac ccgcgtcgcg acgtcgaccc atacctcgag
     4561 gccgcggatc gcaggggcgt ccggatcgcc cacgcgctcg atacgcacgg gcacaacgac
     4621 tacctgagcg ggctctccga gatcgcggcc cggaccgacg ccgagatcct ggcctccgcc
     4681 cacggggagg cccgctaccc gcaccggccc ctcaaggacg ggcagtcgct cgaggtggga
     4741 gaggccgtcg ttgaggcgat gcacaccccc ggccacacgc ccgagcacct ggcgctgctg
     4801 gtacgcgacg cctcggtgga cgatctcgag ccgctgatgc tgctctcggg cggctcgctg
     4861 ctggtgggcg acgtggcccg cccggacttg ctgggagggc gcgacgaggc ccgggaggcg
     4921 gcccgcgtcc tgtgccggac cctggcggag cgcatcctga cgctccccga ccacgtggag
     4981 gtgcacccga cgcacgtctc gggatcgctc tgcgcgggga gcatcgggag ccgcctcacc
     5041 accacgatcg ggtacgagcg gaggacgaat ccttcgctgg cccggcccga ggactgcgtc
     5101 tcgctggagg acctcccggc cgtccccccg tactggcgca ggatgcggtc gcagaacctg
     5161 gagggggtcg cgctgctggg gacggtgacg ccgccgccag ccctccagcc ggaggaggtc
     5221 gagcggcggc tggcggaggg ggccttggcc ctcgacacgc ggacccccga ggacttcgcc
     5281 ggcgggcacg tcccgggcgc gctgagtgcc ggcctgggcg gctcgttccc gacctgggcc
     5341 ggcaccgtgc tgcccgaggg cgcccgcgtc gtgctggtgc tcgacgaccc ggggagcgcg
     5401 atggaggccg cgtggcacct gctccgcatc gggtacgagc cccccctggg tttcctcgag
     5461 ggcggcatgc aggcctggcg agctgacggg aagccggtgc gcgagatgcc agtgatggac
     5521 gtccgcgagc ttcgtcgccg gctggacgac gtcgcggtgc tggacgtgcg ccagccgggg
     5581 gagtggcggg acggccacat cgccggtgcg acccacatca ccggcgcgga gctcgagcgc
     5641 cgcctggacg aggtcccgag ccgccgaccg ctggcggtgc tctgcgggac cggctaccgc
     5701 tcgtccgcgt cggccagcct gctcctgcgg aagggcttcg acgacgtctg gaacgtcctg
     5761 ggcgggagca cagcctggga cgccgccggt tacccgacgg tccgagcgtg agcgacccgg
     5821 cctgcatctt ctgccgcatc gtgtcccgcg acgccctcgc gctcacgatc tacgaggacg
     5881 acgccacgat ggccttcatg gacatcaatc cggccaccga cggccactgc ctcgtgatcc
     5941 ccaagcgcca cgcgccggac gtctggaccc tgcccgagga ggacggggtc gccgtgtggc
     6001 ggtcggtccg gcggctcgcg ggggccgtca gagacgccct ccgcgctgac gggttgaaca
     6061 tcctccagag caacgggcgg gcggcgctgc agaccgtctt ccactaccac gtgcacgtga
     6121 tcccccggta cgtgggggac gggatccacg tgccgctgat gcgggcgccc ggcgaccgga
     6181 gccggctcga cgagttcgcc gacctgatcc gggcgcagct gacctagccg gtcgccgggc
     6241 gcccccggac gtaggggatc accgccgagt agtcgaggcc gccgaggccg gcgtcgcggg
     6301 cggcctccag gccggccagc acggcctccg cgaccggaag ccgggcgccg gcctcggcgg
     6361 cctcgtccag gaccagccgg aggtccttcg ctgcgagccc cagccggaag ttcgggggat
     6421 agcggccgga cacgatcttg tcgcgtttcg agcgggtggt ggcgccgatc ggggagccct
     6481 ccaggacgtc gagtgccgcc tcctcgtcca ccccgagggc gtcggccagc gccagagcct
     6541 cgcccagccc ggccatcagc gctcccagcg tcgagttcac cacgagcttc atggccgccc
     6601 ccgctcccag ctccccgaag cgtcgtggcg tccccatcgc ccggagcgtc ggggcccagc
     6661 ggtccacgag gtcgctcggg ccgcccacga agatccggag cgttccctcc tcggcctggg
     6721 tcacgctgcc cagcacgggc gcgtcgagca cgcccagccc ttccgggagc gcctccgcga
     6781 tctcccgcac ggcccggacc ccgatcgtgg acatctcgat tagcgtggcg tcctcgcgga
     6841 gaccctcggc cagccccccg gtcccgagga cgacctcccg gtccgcgccg gggtcggcca
     6901 gcatcgtgaa cgccgcctcg gctccctccg cggcgtcggc cggcgaggcc gccgcccggg
     6961 ccccggcctc caccaggggc cgggccttct cctcggttcg gttccagacg gccacctcgt
     7021 gcccggcctg gagcagccgc cgggccatgg gagcgcccat ctgccccagt ccgcagaacg
     7081 cgagcacgac catcggcgac agcctacccg cgacccgcgt gggaggatgg cgagatgtcg
     7141 gacgagtacg caccactccc gcgggtccac cccgttatgg cggcgatggc cggcggcgcc
     7201 ctctgggggg cgatggggta cgcggttctg tggggcctct cgccgatcgt ggtggggcgg
     7261 gccttcgtgg tgagcgcgat cggcacgatc ctgttcctgc ccgtccggct cgtgctgtgg
     7321 gtcctgcggt gggtcgaggg ccttgtcggc agaccgttcg agttcgccca ctccaactgg
     7381 tggatcgggg cgctggcggc ggtcgtcggg gccgggatcg ggatcgccct gactctgacg
     7441 gctcgcaccg cgttccgccg gctgcggccg acgccggact gacggccgcg cgtcgaggcc
     7501 gcgcccgggc ggcgggtctg agcgcccgcc ggtgaccgtc gtgggatgat cggaggtggt
     7561 ggccggctct cctcccaccc ctcgaggctg gcgagcgctc cccaccctgg cggtgcgcgc
     7621 cgttcgcgac tccttcgagg accggatctt cggactgggg gcggaggtgg ccttcttctt
     7681 cctcctgtcg ttccctccgg cgctgctgac gatcctggga acgctgggct acgtctcgca
     7741 ggtcctgggg gaggacgtgg cggccgcgat gcgggaccag atcctgagcg tcgcgggcag
     7801 cttcctcacc gaggagacgg tgaccgagac ggtacgaccg ctcgtgaacc ggctgctggc
     7861 cgagggaagg gccgacgtgg cgtcgttcgg gatcgtgctc acgctgtggg ccgcctcccg
     7921 ggccacgacg cgcctgatcg aggcggtcgc gatcgcctac gacgaggagg acgagcgatc
     7981 ggcggtgcgc cgtcgcctcc tctccctggg gatgacggtt gggacgatcg tcgccctcgt
     8041 gctcgtcatc ccgatcctcg tcgccggccc ccgcctgctc gaggcgctgg ccgagcccct
     8101 ggggctgacc gcagccgtcg ctgccgggtg gcgggtcctg tactggccgg tcgtcgcgct
     8161 gatcggcatc gggttgctct cgctcttcta tcatctggcc tccccgctgg agacccggtg
     8221 gcgccgggac gtcccgggag ccttcgcggc ggcgggcgtt tggctcctcg gggggctggg
     8281 gctgcgcgtg tacctgggct tctcgctgga ctcgggggcc tacggtccgc tggcggctcc
     8341 gatcgcggtc ctcctctgga tgtacgtcac cgcgctggcg gtgctgctgg gggccgaggt
     8401 caacgccgag atcgagagga tgtggccgga tcggccgagg aagaagcgcg acgcttaacc
     8461 cgcgacgacg cttcccacca tgccggactc ggcgtgaccc tcgatcgtgc agaggaggtc
     8521 gtaggtcccc tcgtcgagcg tgaagcggtc gcggccccgc cggccgggct ggacgtacac
     8581 atagctcacc tcctctccgc caggggccag gagggtccag tcgtgctcga cccggcccac
     8641 gttccggagt cggacgtcca cggggacgcc ggcccgcagc gcgatctcgg acggatcgaa
     8701 ggcgaactca cgcatctcga cgcggaggcg ctgcgggccg gcgtcccccg gcgcatcatc
     8761 gcaggcgccg agcggggcga gcccgagcgc gatcgctccg agcgcggcag ccctcagccg
     8821 atcgccgccc cggcccgcca ccgtcggacg ttctcgacgt tcgcccgagc cgtcgcgccc
     8881 tccgcgcacg tcgaggggtc gtcgcaggac gggttgccct cgccggtgta gaagtcgaac
     8941 cggcactggg gacagaggac gtccagctcg gccggcaggt aggggcagtc gacgtagtgg
     9001 cagcccttct cgccctcggc cgtggccgga tcgaagccgc agccgggaca ttgacggaac
     9061 tccagccagc ccgccatcgc tcctcctttc gtcgccccac cgtaggaggg cggcccggga
     9121 ggtcccaggg ccgtccggcc cccgggcgcc gggccggtcg gctcaggggg cctcgccccg
     9181 cacgggcgct cccagcgagg ccaggacgat cgcggccagc gcggcgacgg ccgcgcccac
     9241 caggccgatc gtccccagcg gtgaggggac ccacccggcc gcagccaggg cgaccagcgc
     9301 ggctcccgcg ttgaaggccg ccgccctggg ccaggcccac cggcgggtcc gctccaggcg
     9361 ggccctccgc gcctccgagc ccgcggggtc cgacacgggc aggaggtagg accaggctcc
     9421 cagcagcgcc tggatgaaga cgccgagcgt cagggccgcc acgagcgaga gaggccaccc
     9481 agcgttcccg gtgagggcga ggatcagcgc ggccgcggtc acgcccacca gccagagcgg
     9541 caccgagagg aggtgcagcg ccgcccccgg ctcgggcatc cgcgggcgcc tggccacgga
     9601 ccccgccgcc acgtgcacga ggtcggcggc gcccaggagc gcggccccgg cgccgacggc
     9661 cacgagcggc gtccaggatg cgacgtgccc ggccgcctgg aggatcaccc ccagcccaag
     9721 gcctcccacg acgaggcgac cggtggcgtc gggcgcgcgc acccgcagcg tgatggggac
     9781 gagcacgagg agggaccccg cgacggccag ggccaggaac ccctccgcgt tgagggtcac
     9841 gtgggcggcg cggatgtcca ggacatccgc gccgcccacg gtccctgctc ccagcagcgc
     9901 cccgaggacg gcccccagcg tcagcgagag cgccgccccc gcgtaggcga agagggccgc
     9961 cgcgtggcgc cggttgctcc ccgcgcgccg ggcggtcagc acgagccagg tgaggcggag
    10021 ggaccccagc gcgtaggctc cgccgcccac tcccaggagc cacgtgatcc ccaggggcct
    10081 cccgatcgcg atcgaggcgg ccccggccgt gacgagcagg agcggatccc agcgctgggg
    10141 gaggtcgtgc gcgcccgcca gcgcgttggc gaagagcggg agcgtgccgg agacggccga
    10201 gcccgccgcc ccggccagcg cgaggtggat cggcagccac cagcccagcc gatcggccgg
    10261 ggggagcagc agagaggccg ccgccgcgag gagccagatc cgggcgagga cctgggctgc
    10321 ggcggacggc cccgagaacc tcatggatca gatgaacagg gtgaccgcgt cctgcgcctc
    10381 cagcagcgcc gtcgtcgctc cggcgggctc gtcgatgccg tcgatcaggt cctcacggcg
    10441 gaggccgagc atgtccatcg tcatctggca cggtaccagc cggacgccca tgtcgcgcgc
    10501 cagctccagc agctcctggg ggctggccac cttgtgctcc gccgcgagct tcttcatcat
    10561 ccacgggccc atcccggcga agttcatctt ccccagcttc agatgctccg tgcccccgcg
    10621 gttcatcatc gagagcatct tctgcatcca gttctcgccg gtgatccgga cgtcgttctt
    10681 caccaggggg aacagccccc agaacgtgaa gaagacggtc gtgcgcatcc ccatcgcggc
    10741 accgctcgtg gccaggatca gcgtgggcca caccttgtcc aggtccccgc tccaggccat
    10801 gatgaccagc ttcttctcct cggcctcctc cgggacgggg agctccgcga gctctgccat
    10861 ctcggtcacc ccttcctgat caggaaccgg tacacgccgt cctgctccgt ctgctccacg
    10921 agctcgttgc ccgtcgctcc ggcccacgcc ttgaagtccg ggaccgagcc cggatcggtg
    10981 gccaggacct cgatcaggtc tcctgcggcc gcctccttca tggcctgggc ggtcttcacg
    11041 accggcaggg ggcaggacag gcccttcagg tccagcgtct tcgcgatcgt cttcatgcct
    11101 cgctcccttc ttcgctcccg gggctcccca ggaactcggg gatccgcccc tgcggcacgg
    11161 tgttccagta caggcggttg aagagccact tggcggcgtg ccagcgtcgc gacggcttgg
    11221 ggggcaccgg cgggtgctca tagtcgaacc gcagcaccgt cgacctgccc ccgccggtct
    11281 ccaggaagca catcacccgt cctccgtagg aggccttggg cgcggtgccc cggatcagcg
    11341 acgcgatcct gcttgccacg accgggccct cgaagtgggc cgtcgagccg ctcttgctga
    11401 tcggcaggtt cgtggcgtcg ccgatggcga agatccgctc gtgctccttg tgctggagcg
    11461 tggcggggtc ggtcggcatc cacccccccg cgtcgcccag ccccgaggcc gacacgacct
    11521 cctgcccccg gtggggcggg accaggacga gcaggtcgta ctcggccttc tcgccttcca
    11581 gcgactccac cacgtgggcc gccgggtcca cggcctcgac gttgaagaag gtctccaagc
    11641 cgatgccgcg acgctccatg atcggctgga cgagcttcga ggccgactcg atcgtgaacg
    11701 cccggttgag cggcgag
//
LOCUS       DASUTZ010000170        20121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_699905, whole genome shotgun sequence.
ACCESSION   DASUTZ010000170 DASUTZ010000000
VERSION     DASUTZ010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 20121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20121
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699905"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="VF097_07500"
     CDS             complement(<1..1302)
                     /locus_tag="VF097_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX6262675.1"
                     /translation="MTRLPVGAGGLDPTEARARLERDGPNLLPTPAGPALWRELLRQL
                     VHFFALMLWVAGGLALVAGLPQLGIAIFAVVLVNAGFAFAQEFRAERAAQRLRELLPR
                     RVIVIRGGVRREIDAAELVRGDLVLLAAGDRISADLCAMEAHGIWVDSSTLTGESVPS
                     PVEAGDALYAGTFLAEGEGTAVVVEVGDATRLAGIARLAQAGERPTPPLALELRRLVR
                     TISAIAIGVGVSFFGVATLVGTAPTEGIVLAIGITVALVPEALLPTVTLSLAIGAQRM
                     AARRALVRRLESVETLGSTTFICTDKTGTLTMNEMSAVEVWTPAGWAMIRGHGYEPWG
                     EIEAGGPRVGESLREAALAAVRCSTGRVVEREGRWSPLGDPTEVALDVLARRVVGRVL
                     DESEPERVRFPFDPRRRRMSLVVGDRVLVKGAPDAVLPLCRT"
     gene            complement(1343..1696)
                     /locus_tag="VF097_07505"
     CDS             complement(1343..1696)
                     /locus_tag="VF097_07505"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6262676.1"
                     /translation="MKTKAEELIVDAAKVERRPWRPLGGEVGVGYRPLWVGAGSGSYA
                     GVMRMDPGASIRHHAHAYAVHHMWVLEGECAVGERTLEPGAYAFVPAGVEHGIDSAGP
                     EGCTFFYLYLRASLD"
     gene            1836..2423
                     /locus_tag="VF097_07510"
     CDS             1836..2423
                     /locus_tag="VF097_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6262677.1"
                     /translation="MKARDIMSRPAITVTPETPLKRVAELMVDHRIALVPVVDASGNL
                     LGVISEADLLRGNATPQRGEIITSTAREGAAPGTAAMVMTMDVVAAEEAAEVADLFPL
                     MVERHLKGVPVVSDGIVTGVLARRDLLRLLVRTDTDVHDDVMEVLDHVGEEPFSVDVA
                     EGVVTINRWLGPSARRRIEAFVRAIPGVMAVVFKE"
     gene            complement(2485..2901)
                     /locus_tag="VF097_07515"
     CDS             complement(2485..2901)
                     /locus_tag="VF097_07515"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HEX6262678.1"
                     /translation="MTFDVLSEINYLAAVVAALAYFAIGALWYSPVLFSKPWQRAIGM
                     QPQEAQSPNPLLFVLTAVAYFVQAVTLGALARATGGTDLMDGLVLGLFVAIGIVAMGL
                     WVNVSYEQRAASLLWINAGNALVGIVVMSIIMTVWD"
     gene            complement(3294..3443)
                     /locus_tag="VF097_07520"
     CDS             complement(3294..3443)
                     /locus_tag="VF097_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262679.1"
                     /translation="MDRAWERIEDDSYRVCEAWGKPIAREWLAAIPAARFRAEAQARL
                     EASGS"
     gene            3676..4398
                     /locus_tag="VF097_07525"
     CDS             3676..4398
                     /locus_tag="VF097_07525"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX6262680.1"
                     /translation="MSDPGVLDFDWRGRTGPFTIRLAPRVFPPSRTSMLVAETIEIEP
                     GETVIDVGCGSGVLSFVAARLGAARVYGTDVVPEAVEVATENARALGLEDRTEFRAGS
                     LLDPVEDVVADVVIGDVSGIPDSVAELTGWFPGGHAGGPTGAEVPVALIESVADHLRP
                     GGRMYLPTGTIQDEPRVIEAARRIFGAANMESLAEREFPLPSLVGGSKAVARLMADGL
                     LALRRRGSRLLWRLAIWRCIRR"
     gene            4480..4692
                     /locus_tag="VF097_07530"
     CDS             4480..4692
                     /locus_tag="VF097_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262681.1"
                     /translation="MSLFLFAVGAVLTWAIEVEAQGIDLNLVGVILMIVGAVGFVLSM
                     LFWSPWAPYGRRDTPPHDPHGADHVH"
     gene            complement(4754..4921)
                     /locus_tag="VF097_07535"
     CDS             complement(4754..4921)
                     /locus_tag="VF097_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262682.1"
                     /translation="MGLVILLIVLALIFGGFGLLVEGLTWLLIIAGILFLIGLFSGWR
                     TWGGRSTHTRV"
     gene            5018..5341
                     /locus_tag="VF097_07540"
     CDS             5018..5341
                     /locus_tag="VF097_07540"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEX6262683.1"
                     /translation="MDEREAPRPPDDPRREREISEVEQEQMQRLARERRARLAKTIVI
                     LAIAIILIVFVTQNLGEVPIDFVFVTESFPLIWVLIVTTLLGAVAGYLLGRPSKAGRR
                     EPDER"
     gene            5447..5590
                     /locus_tag="VF097_07545"
     CDS             5447..5590
                     /locus_tag="VF097_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3096 domain-containing protein"
                     /protein_id="HEX6262684.1"
                     /translation="MSVNLTLPGIVALAAGILILIFPTILNYIVAIYLIVTGAILIFD
                     IRI"
     gene            5587..6108
                     /locus_tag="VF097_07550"
     CDS             5587..6108
                     /locus_tag="VF097_07550"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HEX6262685.1"
                     /translation="MSGEPERDAETEKERLSRKLGELLEELRVALPGVQVLFAFLLTL
                     PFTSRFSGISDLSRDVYFGSFLAAALASVFLIAPSAYHRMRWRFTDREELEDKRRMLI
                     TSSRLATTGIVLLGAAIAGVTFVIANVLFNVPMALTVSTAITGVAVVLWFALPLTRRA
                     RAREGRRSGSEGP"
     gene            6124..9057
                     /locus_tag="VF097_07555"
     CDS             6124..9057
                     /locus_tag="VF097_07555"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6262686.1"
                     /translation="MSGVHEGLEARLFEHLGAAAVAVDREGVVRLWNDHAETLFGVSR
                     EDAVGHDVVKALHKDELAEVVQALTSASGLDAEVVLRDPLGADRLCFVRVSRLPQPAD
                     GAIALAVDISHRRLVEQRLEAQYAAAMVLAVATDVEKATRRFLREVGRAIGWDAGDVW
                     ILDADTQVMRFADLWAGPGVKGEHLAEAGRTHAFVAGEGLPGRVLASGGPVWIPDVGQ
                     DDNFPRKTLVMEAGVQAAFSFPIRLGDRVLGAMEFFHRDPRLPDEALLKAMDAVGVQF
                     GQFLERATALEAMQESEALKASILKASLEAVIVIDHRGTVIDWNPAAERYFGYSREEA
                     LGWEMAEMIVPEDARARMREGLQRFLETGESRIMGRRHEMVAQRRGGARFPVEVTVVR
                     TDLPGPPTFTGYVRDITERKQTEAALVEGERRLRHALEAGGMGAWEWDLGSGRVEWSA
                     SLERIHGLEKGSFAGTLEAFEADIHPDDRERVREAVDRALAGPDPYRIEYRIVRPDGS
                     QRWLEAIGDVRRDSSGAPVRMHGVCMDVTDRREAEEIRTRLYEGEQAARAEAEAARAR
                     LSFLAEASRILSSSLNLERTLAKLAELAVPRLADWCSVEMVEEGGALKHVALGARDQE
                     LRQEVRAMRERYPPDPEGPQGMGRVLGSGEAIIYPEIPDDLVAAAARDEEHRHRLERL
                     DTRSGMIVPLRARGRTLGAMTFASSARRFDDEDLALAQELADRAAVAIDNARLYEERS
                     AIARSLQRSLLPPALPEIPGVDVAARYEPAGEGFQVGGDFYDVFQRTAGEWAIVIGDV
                     CGKGPEAAAVTGLARHTLHAAWLGEHTPSGSLRLLNEALIKSGTDRFCTVAKGRIAPG
                     QGGATLTVACGGHHRPYVLRASGDIEIVGEPGTLLGVFEDVTITDQTVRLGPGDVVIF
                     YTDGVVDDRGGSADRSPRTLMDFLEGVPRDSAGAVADHIEEQVRKANPDGPQDDVAIV
                     VLRVE"
     gene            complement(9061..9801)
                     /locus_tag="VF097_07560"
     CDS             complement(9061..9801)
                     /locus_tag="VF097_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017581906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HEX6262687.1"
                     /translation="MADESREIELFQRRHEDPIAREQLVEQFRALAEYLARRFQGRGE
                     PVEDLTQVAMIGLLKAIDRFDLGREVKFSTYATATIVGELKRHFRDKGWSVRVPRRLQ
                     ETGLKVSKAMAEVSQEVGRSPTVAEIASHTGLSEEEVLEGMETVHAYSASSLDAPIDE
                     EGRSSHEVMGSEDETIEMIEAWTTVGPAIQALPERERRILYLRFFRGLTQTQIAEEIG
                     ISQMHVSRLLARTIRQLREEVRADEPVE"
     gene            complement(9794..10180)
                     /locus_tag="VF097_07565"
     CDS             complement(9794..10180)
                     /locus_tag="VF097_07565"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6262688.1"
                     /translation="MSDRNSPPVIVEVPAAPEQAYVLRSVVASVAARFDLPLDSIEDL
                     KIAVDEAVAHLLTTGGTRLRMEVVPSDSGLKVTTSTDASQAAWPPEGFEGSLTWQVLT
                     GLTDEAWFAVDGGPILGFTKRALLHG"
     gene            10226..10558
                     /locus_tag="VF097_07570"
     CDS             10226..10558
                     /locus_tag="VF097_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014061729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6262689.1"
                     /translation="MDLTLEVRTENDTPILEVGGEVDIYTAPKLREKLVELIDGGNDR
                     IVVDLEQVGFMDSTGLGSLVAGLKRIRERDGELVIVCTREPVLKVLAITGLDRVFPVH
                     DSVGNALG"
     gene            10562..10954
                     /locus_tag="VF097_07575"
     CDS             10562..10954
                     /locus_tag="VF097_07575"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6262690.1"
                     /translation="MVETRSTLRIPARAAYVGTARIFAAAVARQAGMGEEHVDDLKLA
                     ISEACAAGVRDDGDGSIVIHADLDGSAVSFEVSGQDIPPPPSDDLVSTPESFRRSIGY
                     DVIRSLFPDASLPSKGSAAIRFSVSDES"
     gene            10984..11376
                     /locus_tag="VF097_07580"
     CDS             10984..11376
                     /locus_tag="VF097_07580"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6262691.1"
                     /translation="MLDAQDTDVRILLAPSPESPAAARDAMDRFEGWVDDETLENLRL
                     IVSELVTNSYRHATMRGDERVEVRAGLRDGVLRLEVQDPGHGFAAPDDPVPQAESGWG
                     LYLVDHIADRWGVNRGPDTEVWAELDVD"
     gene            11405..11923
                     /locus_tag="VF097_07585"
     CDS             11405..11923
                     /locus_tag="VF097_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262692.1"
                     /translation="MSQKLIATFAGRESARRAALALERRGIEGSSISLEGAARDSVPI
                     EAGDTRGGDRRLSGDVGARVGVGALIGTVVGGILGLALGLLAFPGAGVWASTIGAAVA
                     GGAVGGIVGGVSGLGTGGAWQRTYAEIREGRVVVSVAHSDPDVVDKAEQTLRELEPDR
                     LERLDDGQRQAS"
     gene            11980..12576
                     /locus_tag="VF097_07590"
     CDS             11980..12576
                     /locus_tag="VF097_07590"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX6262693.1"
                     /translation="MMDRLKDRLASDERMERLRERYGSLQKELIRGLPGERRRRERRA
                     LVMGFLGGMVVGGALTYLLDPVLGRTRRARLADQAAAQAREAARDTERFGRRVASDVE
                     GRIQAARRGTEDYDPPNDVALARKVESEVLGRPDVPKGSVLVNVEHGRVVLRGTVERP
                     EVRDELTRLVLAVDGVDEVENFVQVPGQDAEGIRRLAR"
     gene            12804..12962
                     /locus_tag="VF097_07595"
     CDS             12804..12962
                     /locus_tag="VF097_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262694.1"
                     /translation="MLRCPVCNSIRVVMVLKGESRAFCARCGARWVQNGGRQTDVRRG
                     DTPADHPA"
     gene            complement(13038..14087)
                     /locus_tag="VF097_07600"
     CDS             complement(13038..14087)
                     /locus_tag="VF097_07600"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6262695.1"
                     /translation="MQHVAGEGDEPRGHGGSLPAKQSDNRGRLGRGHPRGGNPRAENH
                     RDHGGVGRRGTSDGEQGLEATAKEVEAEGGTPLALPTDVADPKAVERAAAAVERDLGP
                     IDVWVNNAMTSVFAPFTEIEPEEFRRVTDVTYLGVVHGTLAALRRMMARDRGVIVQVG
                     SALAYRGIPFQSAYCGSKHAIQGFTESVRAELLHEGSGVRLSMVQLPALNTPQFGWVE
                     ARLPNEPQPVPPIYQPEVAARAVVFAADHPRRQVWVGGSTVATILANKLVPGLLDRYL
                     GRTGYGSQQTDEPLDPGRRSNLWEPVPGDHGAHGSFDDRAKDRSIQLWLTTHRGAMVA
                     ALAVGALALVRRALR"
     gene            14046..15296
                     /locus_tag="VF097_07605"
     CDS             14046..15296
                     /locus_tag="VF097_07605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEX6262696.1"
                     /translation="MAPRFVSFARDVLQRSGRDRSGYLAAIVTYYGFLSLFPLLLLAL
                     SVIGFVLADRPELQEEIVDAVAGAVPGLQDVMGDSLESLVDARAASGLIGLAGLVWSG
                     LGATEAAGFAVSRIFRVQPYASFVKRKLWSLWTTIVLGLMAVTALGIVALVGNLPMSG
                     WAAVGLRAGGLLVAFVLDVALFLVGYRLLTQRQGPPFGDLWKGAIFAAAAWGVVKVVG
                     TWYVAGTVSRSSAVYGTLAGAVGILLLIYAASQLLLYGAEVNAVALERAGKDLGSVER
                     GIYVEGDGEMRRTVNGERSTGDLVRSIATDTSQLVRKQIELAKQEMAEGAKAKAVGGA
                     ALAAAAVFGLIAFVFLGVTMAVALRIVWPAWLAWIVTAATFLLLAGVIAGVGVSRMKR
                     PVVPEETKRTVKEDVEWAKAQLRR"
     gene            15293..15640
                     /locus_tag="VF097_07610"
     CDS             15293..15640
                     /locus_tag="VF097_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262697.1"
                     /translation="MREIEEIRGRLSSDIDELQSRMPAAAVWGKRAVGALVGGGAAAT
                     VALLLLRRRRAKRRRQAPVQAVVNVLPQDVARKVADKLDDERVKRMLMVAGGAWLLFK
                     LAELRQLRRMRAA"
     gene            15677..16540
                     /locus_tag="VF097_07615"
     CDS             15677..16540
                     /locus_tag="VF097_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX6262698.1"
                     /translation="MNEALRAWYEPRAAAYPWRSRPTSYRVLVSEVMLQQTQASRVVP
                     EFRAFLRRFPSVRALSAAPLDEVLRAWSGLGYDRRAVWLHRAARAIVDEHGGRLPRDV
                     EALRALPGVGSYTAAAVASIAFGEPVPALDVNVGRVVARFRLGREVHEEGSDRVLEAA
                     SAWIDRDDPGAWNQALMDLGREVCRPTPRCAACPLAPGCAFRRAGRRPARAPRRQDRF
                     EGSFRQVRGRVLAVLRERGSASLGSLEAEIGDGRVARAVRALADDGLVSAGPAALAGR
                     ASGRVRLGSIR"
     gene            16537..18108
                     /locus_tag="VF097_07620"
     CDS             16537..18108
                     /locus_tag="VF097_07620"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX6262699.1"
                     /translation="MSDLLVRAARVHGAPGADAVAISGHRIDAVGPWDEIRWLGGETV
                     EVPAGLILPGFQDAHIHVAEGGRILSLCDLHDVSGLAAYLEAVVTYGRRHPDRPWIEG
                     GGWTMSDFPGGTPHREPLDAAVPDRPVLLESRDGHDAWVNSRALEAAGIDRRTPDPPR
                     GRIGRDPDGETSGVLHEAAVRLVADLLPEPSGREWEEAILAGQGHLLALGVTAWQDVN
                     VFPEWVEAYRRLADDGRLIGRATIGMHWDPARGLEQIAELVQRRGSSGRLRVETVKLF
                     QDGIVENGSASMLEPYTGSESRGVSNFEPEELNEIVSALDAEGFQVHVHAIGDRAVRE
                     ALDAFEVARVANGVRDARHTIAHIQVVHPDDVPRFAELDVIPNGQPYWACLEPQMVEL
                     TLPVLGPERSARQYPFGSLLRTGARLAFGSDWTVSTADPLAEMAVAVTRQPPDEPEAE
                     VLLPEERLTPGEAIEAFTIGSARVNRLEDVTAAVEPGRLADLVVVDRDILEPGSMEGA
                     SAAVTIVGGRIVHGP"
     gene            complement(18112..19680)
                     /locus_tag="VF097_07625"
     CDS             complement(18112..19680)
                     /locus_tag="VF097_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015801882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6262700.1"
                     /translation="MGEAGFRVTYATMSADDDALHEAYERGIEEARSWLGGQHPFLVN
                     GEPREGESWQETRSPIDRDVVIGTFAEASKQDVDDAISAAQTFAPEWAATPWDERVAL
                     LRKAADLISERRNELSALMAIEVGKARLEALGDVEETADLIRYYCHEMERNDGFAKPM
                     GQLSEAEKTRDVMRPYGVWGVISPFNFPMALAGGPSGAALVAGNTVVLKPSGQGYFTG
                     LKLGEALLDAGVPPAAFQVLPGKGSVAGAALVADPRLGGITFTGSYEVGMGIYKSFTQ
                     DYPRPVICEMGGKNPVIVTAKADLEKAAEGIKRSAFGFGGQKCSAASRVYVEAPVRDE
                     LVSALLEKTSEIRIGDPLERDVWLGPVIDEKAVARYEEAVAEARANGEVLHGGERLTE
                     GDLERGNFVEPTIVTAPRDSWLWKRELFVPFIAVDAVGSLDEALELANDTEYGLTAGL
                     FSEDRGEIDRFLAGIEAGVVYVNRRAGATTGAWPGVQPFGGWKGSGTSGKAGGGDYYV
                     QQFMREQSQTLVAD"
     gene            complement(19683..>20121)
                     /locus_tag="VF097_07630"
     CDS             complement(19683..>20121)
                     /locus_tag="VF097_07630"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX6262701.1"
                     /translation="EGDSPADCARKLRDAGAHVVGVNCLRPPGHTLPILREMREAVDG
                     WIAGQPVAYRTPAESPDFTSLPEFPEGLDPLQLPRSEMAAWAVEASDLGVNLIGSCCG
                     SVAAHVRAMALALGKLPEDDREWRVDYERPMSAYEYYEHGKEA"
BASE COUNT         2842 a         6986 c         7284 g         3009 t
ORIGIN      
        1 ggtcctgcac aggggcagga ccgcgtccgg ggcccccttg accagcaccc gatcgccgac
       61 aaccagcgac atccggcggc gcctgggatc gaaggggaac cgcacgcgct cgggctcgct
      121 ctcgtccagg acccgtccca ccacccgccg ggccaagacg tcgagggcca cctcggtggg
      181 atcgcccaat ggcgaccacc gtccctcccg ctccaccacc cgacccgtcg agcagcggac
      241 ggcggccagg gccgcctccc tgagcgactc gccgacccgg ggtccccccg cctcgatctc
      301 cccccagggc tcgtagccat ggcctcggat catggcccac ccggccgggg tccagacctc
      361 gaccgccgac atctcgttca tcgtcagcgt gccggtcttg tcggtgcaga tgaaggtcgt
      421 ggaccccagg gtctccacgg actcgagacg ccgcaccagg gccctcctgg ctgccatgcg
      481 ctgggcgccg atcgcgagcg acagggtgac ggtcggcaat agcgcctcgg gcacgagcgc
      541 caccgtgatc ccgatggcga gcacgatccc ctcggtgggc gccgtgccca ccagggtggc
      601 cacgccgaag aaggagaccc cgacgccgat cgcgatggcc gagatcgtcc tcaccaggcg
      661 gcggagctcc agcgccagcg gaggggtggg gcgctcgccc gcctgagcca gccgggcgat
      721 ccccgcgagc cgcgtggcgt cccccacctc gaccaccacg gccgttccct cgccctccgc
      781 gaggaaggtc cccgcgtaga gcgcatcccc cgcctcgacc ggcgatggga cgctctcgcc
      841 ggtcagcgtc gagctgtcca cccatatgcc gtgggcctcc atggcgcaga ggtcggccga
      901 gatccgatct ccggccgcga gcaggacgag gtcccctcgc acgagctccg ccgcatcgat
      961 ctccctccgc acgccgcccc ggatcacgat gacccggcgg gggaggagtt cgcggagccg
     1021 ctgtgcggcc cgctccgccc ggaactcctg ggcgaaggcg aagccggcgt tcaccaggac
     1081 gacggcgaag atggcgatcc ccagctgggg gagcccggcc acgagggcca ggccccccgc
     1141 gacccacagc atgagcgcga agaagtggac gagctgacgc aggagctccc gccacagggc
     1201 cggcccggcg ggcgtgggca ggaggttcgg gccgtcgcgc tccaagcggg cccgggcctc
     1261 cgtcgggtcg agcccccccg cgccgaccgg gaggcgtgtc atggagatct cccggacggc
     1321 ccccgccacg actcgttccc cctcagtcca gagaggctcg gaggtacagg tagaagaacg
     1381 tgcacccctc cgggcccgcc gagtcgatgc cgtgctcgac gccggccggg acgaatgcgt
     1441 aggctcccgg ctccagggtc cgctccccga cggcgcactc gccttccagc acccacatgt
     1501 gatggacggc gtacgcatgc gcgtgatggc ggatcgacgc ccccgggtcc atccgcatca
     1561 cgcccgcata ggaacccgag cccgccccga cccagagagg tcggtagccc acgccgacct
     1621 ccccgcccag cggtctccac ggacggcgct ccaccttggc ggcgtcgacg atcagctcct
     1681 ccgccttcgt cttcatggtt cctcctctcc ctcaacggtg catgcccgga gacggagggg
     1741 gcagggctca gggtcccttc cgacgtgcga ccttcggccc atgcccgctc cgggccccgg
     1801 cgggagagtg ggggcgacgg aaggagcgtg gtcgcatgaa agcacgcgac atcatgtcca
     1861 ggcccgccat caccgtgacg cccgagaccc cgctgaaacg cgtcgccgag ctcatggtgg
     1921 accaccgcat cgccctcgtg cccgtggtgg acgcctcggg gaacctcctc ggcgtcatct
     1981 cggaggcaga tctgctccgg gggaacgcga cgccgcaacg cggcgagatc atcactagta
     2041 cggctcgcga gggagccgcg ccggggacgg cggcgatggt catgacgatg gacgtcgtgg
     2101 ccgcggagga ggccgcggag gtcgcggacc tcttccccct gatggtcgag cgacacctca
     2161 agggggtccc cgtcgtctcc gacggcatcg tcacgggcgt cctggcgcgc agggacctgc
     2221 tcagactcct cgtgcggacg gacaccgacg tgcacgacga cgtgatggag gtgctcgacc
     2281 acgtgggcga ggagcccttc agcgtggacg tggccgaggg cgtcgtgacg atcaaccggt
     2341 ggctggggcc ctcagcgcgc cgcaggatcg aggccttcgt gcgcgcgatc ccgggcgtga
     2401 tggcggtcgt cttcaaggaa tagtggcata ccggatagga ccccgggacg aggccggcgg
     2461 gccgggggct gccggtccgc ctgctcagtc ccagaccgtc atgatgatcg acatcacgac
     2521 gatccccacc agcgcgttgc cggcattgat ccacagcagc gaagccgctc gctgctcgta
     2581 ggagacgttg acccagagcc ccatggcaac gatgccgatc gccacgaaga ggccgagaac
     2641 gagcccgtcc atcaggtccg tcccaccggt cgcgcgagcc aacgccccga gtgtgaccgc
     2701 ctgcacgaag taggccacgg ccgtcaggac gaagagcaac gggttcgggg actgcgcctc
     2761 ttgcggctgc atcccgatcg cccgctgcca cggcttcgag aagaggacgg gcgagtacca
     2821 gagcgccccg atcgcgaagt aggcgagcgc ggccaccacc gccgccagat agttgatctc
     2881 gctgagcacg tcgaacgtca aggtgtcccc cttgttccgc ttgccgctca gttatagacg
     2941 ggggcgcacg gaagcgccag cggagagctc agcactgctt tcttgccgtg cacttgggac
     3001 cggcccacgt cccctccagg gtcacctggg agatcgcgat agcgctcctg gtcagagcct
     3061 gctccgtcac cacgacgctg tcgacgattc gcccggtgtc ctcgagggcc gggagctcga
     3121 atcggattga cgcgagggtt gcgccggcgg cctgagggaa aggtaggttc ccgaggaggt
     3181 gagcgcatgt tcatcccgat cggcatcggg acgctgatcg tcatcatcgt cctgatcgcc
     3241 ctgctgacct gaccaccgga gggcggcgac ccagggtcgc cgcctcttcc gtctcagctt
     3301 cctgatgcct ccagcctggc ttgggcctcc gcgcggaatc gggcggccgg gatcgccgcc
     3361 agccactcac gggcgatcgg cttcccccac gcttcgcata cccggtagga gtcgtcctcg
     3421 atccgctccc acgcgcggtc cacgtcggcg aggcgatcct ccagctgctc gacgatcgag
     3481 agatccttcg ccctctcgaa cgtcccctct cgatcatcgc cccccggagc tgctcgagct
     3541 ccaggcgatc cttcgacagg cgcttccgtg tctcgtcccg gttgatcccc tctcctctag
     3601 cgtatcggat cttccggtca tcgccccccg gcaggggtgg cgcgtagccc cgatcgctgg
     3661 gaggatggtc cccaaatgag cgatccgggg gtgctcgact tcgactggcg gggcaggacc
     3721 ggtccgttca cgatccgcct cgcccccagg gtcttccctc ccagccgcac cagcatgctg
     3781 gtcgccgaga cgatcgagat cgagcccggc gagaccgtga tcgacgtggg gtgcggctcc
     3841 ggggtgctca gcttcgtcgc cgcgcgtctg ggcgcagccc gcgtgtacgg gacggacgtg
     3901 gtccccgagg ccgtggaggt ggcgacggag aacgcgcgtg cgctcggcct cgaggatcgc
     3961 accgagttcc gcgctggcag cctgctggat ccggttgagg acgtggtggc cgacgtcgtg
     4021 atcggagacg tctcagggat cccggactcg gtcgccgagc tcacggggtg gttcccgggc
     4081 gggcacgcgg ggggccccac cggcgccgag gttcccgtgg ccctgatcga atccgtcgcc
     4141 gaccacctgc gtcccggcgg gcgcatgtat ctgccgacgg gcacgatcca ggacgagccc
     4201 cgcgtgatcg aggccgcccg aaggatcttt ggggcggcga acatggagtc cctggccgag
     4261 cgggagttcc ccctgccgtc gctcgtgggc gggtcgaagg cggtggcccg cttgatggcc
     4321 gacggcctcc tggccctccg ccgccgcggc agcaggcttc tctggcgtct cgccatctgg
     4381 cgctgcatcc gccgctgacg ccgggtttcc cacgccccct cccagggaat ccaacggagc
     4441 gaacaaccaa gcgggaggtg acgagacgtg ggtatcggtg tgagcctgtt cctgttcgcc
     4501 gtgggcgccg tgctgacctg ggccatcgag gtcgaggcac aggggatcga cctcaacctc
     4561 gtgggtgtga tcctcatgat cgtgggagcc gtggggttcg tgctctcgat gctcttctgg
     4621 agcccgtggg caccctacgg gcggcgcgac acgccgccgc acgatccgca cggcgcggat
     4681 cacgttcact gacggacgcg aatgcgaaga agccccggcc ttccggccgg ggcttcttcg
     4741 tgtctccagt cggtcagacc cgcgtatggg tggatcggcc accccaggtc cgccagccgc
     4801 tgaacagccc gatgaggaag aggatccccg cgatgatgag gagccaggtc aggccctcga
     4861 cgaggagccc gaagcctccg aagatcagcg ccagcacgat cagcaggatc acaaggccca
     4921 tggattcgca cctccttctg atgttcagca gcgaacctac ccgggtcgcc tcctccgcaa
     4981 tcctgggcgg tctcgggtat agacttcgat gcatcacatg gacgagcgag aagctccgcg
     5041 gccgcccgat gacccgcggc gggagcgcga gatcagcgag gtcgagcagg agcagatgca
     5101 gcgcctcgcc cgcgagcgcc gggcgcggct ggcgaagacc atcgtcatcc tggcgatcgc
     5161 gatcatcctg atcgtcttcg tgacccagaa cctgggcgag gtccccatcg acttcgtgtt
     5221 cgtcaccgag agcttccctc tgatctgggt gctgatcgtc acgacgctcc tgggcgccgt
     5281 cgccggctac ctgctggggc ggccatccaa ggccgggcgc cgggaaccgg acgagcgctg
     5341 aacggtttgc tctgctcgcg cagagggtag gccagggccg tccggatcac aggaggaagg
     5401 catgctcgga atcgcgaccg cgctcgtggc tcaggccgcc ggggacatct ccgtcaacct
     5461 caccctgccg gggatcgtgg ccctcgccgc ggggatcctg atcctcatct tccctacgat
     5521 cctcaactac atcgtggcga tctacctcat cgtgaccggg gcgatcctga tcttcgacat
     5581 ccggatatga gcggggagcc ggagcgggac gccgagaccg agaaggagcg actctcccgg
     5641 aagctgggcg agctgctcga ggagctccgc gtcgccctcc ccggcgtcca ggtgctgttc
     5701 gccttcctgc tgaccctccc gttcacgagc cggttctcgg gcatctccga cctctcccgg
     5761 gacgtctact tcggctcctt cctcgccgcg gcgctggcct cggtgttcct gatcgcgccg
     5821 tccgcctatc accggatgcg gtggcgcttc accgacaggg aggagctgga ggacaagcgg
     5881 aggatgctga tcacatccag caggctggcc acgacgggca tcgtcctgct cggagccgcg
     5941 atcgccgggg tgaccttcgt catcgcgaac gtactgttca atgtgccgat ggccctcacg
     6001 gtcagcaccg cgatcaccgg cgtggcggtc gtgctctggt tcgccctccc cctcacgaga
     6061 agggcccggg cgagggaagg gcgcagatca gggtccgagg gcccgtagta tcctcgcttt
     6121 cccatgtcgg gcgtccacga aggcctcgaa gcccgcctct tcgagcacct cggcgccgcc
     6181 gcggtcgccg tcgaccgcga gggggtcgta aggctctgga acgaccacgc cgagaccctc
     6241 ttcggcgtct ctcgcgagga cgccgtgggc cacgacgtgg tcaaggccct ccataaggac
     6301 gagctcgccg aggtggtgca agccctcacc tcggccagcg gcctcgacgc cgaggtcgtt
     6361 ctgcgggacc cgctcggtgc cgaccgcctc tgcttcgtcc gcgtgtcgcg cttgccgcag
     6421 ccggcggacg gagcgatcgc gctcgccgtc gacatcagcc accggcggct cgtggagcag
     6481 cgcctcgagg ctcagtacgc ggcggcgatg gtcctcgcag tggcgacgga cgtcgagaag
     6541 gccacccgca gattcctccg cgaggtgggg cgggcgatcg gatgggacgc cggagacgtc
     6601 tggatcctcg acgcggacac gcaggtcatg cggttcgccg atctctgggc gggcccgggc
     6661 gtgaaggggg agcatctcgc cgaggccggc cgcacacacg ccttcgtggc cggcgagggg
     6721 ctcccggggc gcgtgctggc ctcgggcggc ccggtctgga ttcccgacgt gggccaggac
     6781 gacaacttcc ctcgcaagac cctggtgatg gaggccggcg tgcaagccgc gttctcgttc
     6841 cccatcaggc tcggagaccg ggtcctcggc gccatggagt tcttccaccg cgacccccgt
     6901 cttcccgacg aggccctcct gaaggcgatg gacgcggtgg gcgtccagtt tggccagttc
     6961 ctggagcggg ccacggcgct ggaggcgatg caggagagcg aagccctcaa ggcctcgatc
     7021 ctgaaagcct cgctcgaggc cgtgatcgtg atcgaccatc gggggacggt gatcgactgg
     7081 aaccccgccg ccgagcggta cttcggatac tcccgcgagg aagccctggg ctgggagatg
     7141 gccgagatga tcgtccccga ggacgcccgc gcgaggatgc gtgagggcct gcagcgcttc
     7201 ctggagaccg gggagtcgcg gatcatgggc aggcggcacg agatggtcgc ccagcgccgg
     7261 ggcggcgccc gcttcccggt cgaggtcacc gtggtgcgca cggacctccc cggtcccccc
     7321 accttcaccg gctacgtccg ggacatcacc gagcggaagc agacggaggc ggcgctggtg
     7381 gagggcgagc gcaggctccg tcacgcgctc gaggctggcg ggatgggcgc gtgggagtgg
     7441 gacctggggt cgggacgcgt cgagtggtcc gccagcctgg agcggatcca cggactcgag
     7501 aagggatcct tcgccgggac gctggaggcc ttcgaggccg acatccaccc ggacgaccga
     7561 gagcgggtcc gggaggcggt cgatcgggcg ctggcgggcc ccgaccccta ccggatcgag
     7621 taccggatcg tgcggccgga tggctcccag cgctggctgg aagcgatcgg ggacgtccgg
     7681 cgcgattcct cgggcgcgcc cgtccggatg cacggcgtct gcatggacgt gacggatcgg
     7741 cgcgaggccg aggagatcag gacccgtctc tacgagggcg agcaagcggc ccgggcagag
     7801 gccgaggcgg ccagggctcg cctgtcgttc ctggccgagg cgagccgcat actgagctcg
     7861 tcgctcaatc tggagcgcac gctggctaag ctcgcggagc tcgcggtgcc ccgcctggcg
     7921 gactggtgca gcgtggagat ggtggaggag gggggcgccc tcaagcacgt cgccctcggg
     7981 gctcgcgacc aagagctccg gcaggaggtc cgggcgatgc gggagcggta cccgcccgat
     8041 cccgagggtc cccagggcat gggtcgggtc ctcgggagcg gcgaggcgat catctacccc
     8101 gagatcccgg acgatctggt ggccgccgcg gcgcgggacg aggagcaccg gcacaggctg
     8161 gagcggctgg acacccgctc cgggatgatc gtgccgctca gggctcgcgg gcggacgttg
     8221 ggtgcgatga ccttcgcctc gagcgcgcgt cgcttcgacg acgaggacct ggcgctggcc
     8281 caggagctgg ccgatcgggc cgccgtggcg atcgacaacg cccggctgta cgaggagcgg
     8341 agcgcgatcg cccgcagcct ccagcgcagc ctcctgcccc cggccctccc ggagatcccg
     8401 ggcgtcgacg tcgcggctcg gtacgagccc gccggggagg ggttccaggt ggggggcgac
     8461 ttctacgacg tgttccagcg caccgcgggg gagtgggcga tcgtgatcgg cgacgtctgc
     8521 gggaagggcc ccgaggcggc cgccgtgacc ggcctggccc ggcacacgct ccacgccgcc
     8581 tggctggggg agcacacgcc cagcggctca ctccggctcc tcaacgaggc gctgatcaag
     8641 tcggggaccg accgcttctg cacggtggcc aagggccgca tcgccccggg gcagggtggg
     8701 gcgacgctga cggtcgcctg cggcggccac caccgcccct acgtcctccg ggcgagcggc
     8761 gacatcgaga tcgtaggcga gcccgggacc ctcctgggcg tcttcgagga cgtcacgatc
     8821 accgaccaga ccgtgcggct cggcccaggc gacgtggtga tcttctacac cgacggcgtc
     8881 gtggatgatc ggggcggttc ggccgaccgg tccccgcgca cgctgatgga cttcctggag
     8941 ggcgtccctc gggactcggc aggggccgtc gccgaccaca tcgaggagca ggtgcgcaag
     9001 gcgaacccgg acgggccgca ggacgacgtg gcgatcgtgg tcctccgcgt ggagtgacgg
     9061 tcactccacg ggctcgtccg cccgaacctc ctcgcggagc tggcggatcg tccgcgctag
     9121 cagccgggag acgtgcatct gagagatgcc gatctcctcg gcgatctgtg tttgcgtgag
     9181 cccccggaag aaccgcaggt agaggatcct ccgttcccgc tcggggagcg cctggatggc
     9241 ggggcccacg gtggtccagg cctcgatcat ctcgatcgtc tcgtcctcgg agcccatcac
     9301 ctcgtgtgag ctgcggccct cctcgtcgat cggggcgtcc agcgaggagg cggagtaggc
     9361 gtggacggtc tccatgccct ccaggacctc ttcctcggag agccccgtgt gggaggcgat
     9421 ctcggccacg gtgggcgagc gtcccacctc ctgcgagacc tccgccatgg ccttgctgac
     9481 cttgaggccg gtctcctgaa ggcggcgcgg gacgcgtacg gaccagccct tgtcgcggaa
     9541 gtgccgcttc agctccccga cgatcgtggc cgtggcgtag gtcgagaact tcacctcgcg
     9601 cccgaggtcg aagcggtcga tcgccttcag caggccgatc atcgccacct gggtcaggtc
     9661 ctcgaccggc tcgccacgcc cctggaagcg ccgagccagg tactcggcca gcgcgcggaa
     9721 ctgctcgacg agctgctccc tcgcgatggg gtcctcgtgc cgccgctgga acagctcgat
     9781 ctcgcgggac tcgtcagcca tggaggaggg ctctcttcgt gaagccgagg atcggtcccc
     9841 cgtcgacggc gaaccacgcc tcgtccgtca ggccggtcag cacctgccag gtgagcgatc
     9901 cttcgaagcc ctcggggggc caggccgcct gggaggcgtc ggtcgaagtg gttaccttga
     9961 gacccgaatc cgaggggacg acctccatgc ggagccgcgt gccgccggtc gtgagcaggt
    10021 gggccacggc ctcgtccacg gcgatcttga ggtcctcgat actgtcgagc ggcaggtcga
    10081 aacgcgcggc caccgaggcc acgacgcttc gcagcacgta ggcctgctcc ggggcggcag
    10141 ggacctccac gatgacgggg ggcgagttac ggtcgctcac ggaccgccta tactacaggc
    10201 cgccgatgcg acgaggtgag gagccatcga cctgaccctc gaagtgcgaa ccgagaacga
    10261 cacccccatc ctggaggttg gaggcgaggt cgacatctac accgcgccca agctccggga
    10321 gaagctcgtc gagctgatcg acgggggcaa cgaccggatc gtggtggacc tcgagcaggt
    10381 gggcttcatg gactccaccg gcctggggag cctcgtcgcc ggcctgaagc ggatccggga
    10441 gcgggacggc gagctcgtga tcgtctgcac ccgcgagccg gtcctgaagg tcctggcgat
    10501 caccggcctc gaccgtgtgt tccccgtgca cgactccgtc gggaacgcgc tgggctgagc
    10561 gttggtcgag acccgttcca cgctgcggat ccccgcccga gcggcctacg tggggacggc
    10621 tcgcatcttc gccgcggccg ttgcccggca ggccggcatg ggcgaggagc acgtggacga
    10681 cctcaaactc gccatctcgg aggcgtgcgc cgccggggtc cgtgatgacg gcgacggcag
    10741 catcgtgatc cacgccgacc tcgacggatc cgcggtgtcg ttcgaggtgt ccggtcagga
    10801 catcccccct cccccttcgg acgatctcgt gtccacaccc gaatccttcc ggcgcagcat
    10861 cgggtacgac gtgatccggt ctctcttccc ggacgcctcc ctcccgtcga agggctcggc
    10921 ggccatccgg ttttcggtct cggacgagtc gtgagcccga gacggggtat cagtagacag
    10981 gtgatgttgg acgcgcagga cacggacgtg aggatcctcc tcgccccgtc ccccgagtcc
    11041 cccgcagctg cgcgcgacgc gatggatcga ttcgaggggt gggtcgacga cgagaccctg
    11101 gagaacctcc ggctcatcgt gagcgagctg gtgacgaaca gctaccggca cgcgacgatg
    11161 cgaggcgatg agcgtgtcga ggttcgtgcc ggcctgcgcg acggtgtgct ccggctcgag
    11221 gtccaggatc cgggccacgg gttcgcggcg cccgacgacc ccgtgcccca ggccgagtcc
    11281 ggctgggggc tgtacctcgt cgatcacatc gcggaccgct ggggcgtgaa tcgagggccg
    11341 gacaccgagg tctgggccga gctcgacgtg gactgagcgg cccccgagcg ggtagggacc
    11401 caccatgtct cagaagctga tcgcgacctt cgccggccgg gagagcgcac gccgagccgc
    11461 tctcgctctg gagcgacgag ggatcgaggg gtccagcatc tcgctcgagg gcgcagcccg
    11521 cgattccgtc cccatcgagg ccggcgacac tcgcgggggc gatcgtcgtc tcagcgggga
    11581 cgtgggcgcg cgggtcgggg tcggggcgct gatcggaacg gttgtcggcg ggatcctcgg
    11641 gctcgccctg gggctcctgg ccttcccggg ggcgggggtc tgggcctcga ccatcggggc
    11701 cgccgtcgcc ggaggggccg tcggcgggat cgtcggaggc gtctcgggtc tcgggacggg
    11761 cggggcgtgg caacgaacct acgccgagat ccgggaggga cgggttgtcg tcagcgtcgc
    11821 gcattcggat cccgacgttg tcgacaaggc cgagcagacc ctccgggagc tggagccgga
    11881 ccggctcgag cggttggacg acgggcagcg gcaggcgtcc tagctcgctc tcgtttgcca
    11941 gcatccgctc cgggtaccac cggacggggg tgactgacga tgatggatcg tctgaaggat
    12001 cgactggcat ccgatgagcg gatggagcgt ctgcgcgaac gctatgggtc tctccagaag
    12061 gagctgatcc gagggctccc cggggagcgg cgcaggagag agcgccgtgc cctggtgatg
    12121 ggcttccttg gaggcatggt cgtcggcgga gcgctcacgt acttgctgga tccggtgctt
    12181 ggccgcacgc gccgggccag gctggccgac caggccgcgg ctcaggctcg cgaggcggcg
    12241 agggacacgg agcgcttcgg gcgaagggtc gcatcggacg tggaggggcg catacaggcc
    12301 gcccgccgcg ggactgagga ctacgatccg ccgaacgacg tcgcgctcgc ccgcaaggtc
    12361 gagagcgagg tcctggggcg gccggacgtc ccgaagggat cggtgctcgt caacgtggag
    12421 cacggacggg tggtcctgag gggcaccgtt gagcgccccg aggtgcggga cgagctgacc
    12481 cgcctggtgc tggcggtgga cggggtggac gaggtggaga acttcgtcca ggtgcccggc
    12541 caggacgcgg aggggattcg gcgcctggcg aggtgagccc agacacgaaa ggggccgcgc
    12601 tgagcgcggc cccttgtcgt cgagagaggg aggtctcccg cggtcggttg tgccctcaac
    12661 attcccacgg atccggtctc caaacctttc ttccgggatc tcctaggggg ctccctggag
    12721 gcgtttcgtg cccgggcggt gagggaatga acgcacgacc ctggaagccc ctctcggggc
    12781 ccggagccaa ggaggagacc ctgatgctgc gctgtcccgt ctgcaactcg atccgcgttg
    12841 tgatggtcct gaagggtgag agccgggcgt tctgcgcccg ctgtggcgcg cggtgggtgc
    12901 agaacggcgg ccgtcagacc gacgtccgcc gtggggacac gccggccgac catccggcct
    12961 gagcgatggc cgaggcggcc gagaacgtcc cgaacggtcg tggaggacca ggcgaaggtg
    13021 gccccggttc ctctgactca gcggagggcc cggcgaacga gggcgagggc gcccacggcc
    13081 aaagccgcga ccatcgctcc tcgatgggtg gtgagccaca gctggatgct gcggtccttg
    13141 gcgcggtcgt cgaacgagcc gtgcgcgccg tggtcgcctg ggacgggctc ccagaggttc
    13201 gagcgacggc cggggtccag cggctcgtcg gtctgctgcg agccatagcc cgtcctcccc
    13261 aggtaccgat cgagtaggcc ggggaccagc ttgttcgcga ggatcgtggc cacggtggag
    13321 cccccgaccc agacctggcg cctggggtgg tcggcggcga agacgacggc ccgcgccgcc
    13381 acctcgggct ggtagatggg cggcacgggc tggggctcgt tcgggaggcg cgcctccacc
    13441 cagccgaact ggggcgtgtt gagcgccggc agctggacca tcgagaggcg cacgccgctc
    13501 ccttcgtgga ggagctcagc ccggaccgac tcggtgaagc cctggatggc gtgcttggag
    13561 ccgcagtagg ccgactggaa ggggatcccc cggtaggcga gggccgagcc gacctggacg
    13621 atcacccctc ggtcgcgggc catcatgcgt cggagggcgg ccagggtgcc gtggaccacg
    13681 cccaggtagg tcacgtcggt gacgcggcgg aattcctcgg gctcgatctc ggtgaagggg
    13741 gcgaagaccg acgtcatcgc gttgttgacc cagacgtcga tcggtcccag atcccgctcg
    13801 accgccgccg ccgcccgctc cacggccttg gggtcggcga cgtcggtggg gagggcgaga
    13861 ggggttccgc cctcggcctc gacctccttg gcggtggcct cgaggccttg ttcgccgtcg
    13921 ctcgtcccac gccggccgac gcccccgtga tcacgatggt tctcggcacg agggttccct
    13981 cccctcgggt ggccccgccc taaacgccct cggttatcgc tctgcttcgc gggtaggctt
    14041 cccccgtggc ccctcggttc gtctccttcg cccgcgacgt gctgcagcgc tccggtcgcg
    14101 accggagcgg gtacctggcg gcaatcgtga catactacgg gttcctctcg ctcttccccc
    14161 tgctcctgct ggccctctcg gtgatcggct tcgtcctggc ggaccgtccg gagcttcagg
    14221 aagagatcgt ggacgcggtc gccggcgccg tgcccggcct gcaggacgtc atgggcgaca
    14281 gcctggagtc gctggtggac gcccgggccg cctcggggct gatcgggctg gccgggctcg
    14341 tgtggtccgg gctcggggcg accgaggccg ccgggttcgc cgtcagccgg atcttccgcg
    14401 tccagcccta cgcctcgttc gtcaagcgga agctctggtc gctgtggacg acgatcgtgc
    14461 tcgggctgat ggcggtgacc gccctcggga tcgtggcgct ggtggggaac ctgccgatgt
    14521 ccggatgggc cgccgtgggc ctgcgcgcgg gcggcttgct cgtggccttc gtcctggacg
    14581 tcgcgctgtt cctggtgggg taccggctcc tcacccagcg gcaggggccg cccttcggcg
    14641 atctgtggaa gggggcgatc ttcgccgcgg cggcctgggg ggtcgtgaag gtcgtcggaa
    14701 cctggtacgt cgctggcacc gtctcgcggt cgagcgccgt gtacgggacg ctcgccggtg
    14761 cggtggggat cctgctgctg atctacgctg cgtcccagct cctcctgtac ggggcggagg
    14821 tcaacgcggt tgcgttggaa cgagcgggga aggaccttgg gagcgtggag cgagggatct
    14881 acgtggaagg ggatggtgag atgcgacgaa cggtgaacgg agagcggtcc accggggatc
    14941 tggtgcggtc gatcgcgacg gacacgtcgc agctggtgcg gaagcagatc gagctcgcca
    15001 agcaggagat ggccgagggg gccaaggcga aggccgtggg cggggccgcg ctggccgcgg
    15061 cggccgtctt cggcctgatc gcgttcgtgt tcctgggcgt caccatggcc gtggcgctcc
    15121 ggatcgtctg gccggcctgg ctggcctgga tcgtgaccgc ggcgacgttc ctgctgctgg
    15181 ccggcgtgat cgccggggtg ggcgtgagcc ggatgaagcg gcccgtggtg cccgaggaga
    15241 cgaagcgcac ggtgaaggag gacgtggaat gggccaaagc gcagctgaga cggtgaggga
    15301 gattgaggag atccgtgggc ggctctcctc ggacatcgac gagctccaga gccgcatgcc
    15361 cgcggccgcc gtctggggga agcgggccgt gggcgccctc gtcgggggag gggccgcggc
    15421 gacggtcgcg ctcctgctcc tgcggaggcg gcgggccaag cggcggcgtc aggctcccgt
    15481 ccaggcggtt gtcaacgtcc tcccccagga cgtcgccagg aaggtggcgg acaagctcga
    15541 cgacgagcgg gtgaagcgga tgctgatggt ggcgggggga gcctggctcc tgttcaagct
    15601 cgccgagctc cgccagctcc gccgcatgcg ggccgcctga gtcaagccgg ccgcgcctcc
    15661 gtgcgaaggt atgcgcgtga acgaggcgct gagggcttgg tacgagccgc gagcggccgc
    15721 ctacccgtgg cggtcccgcc ccacctcgta ccgcgtgctc gtctccgagg tgatgctcca
    15781 gcagacgcag gcctcgaggg tcgtgcctga gttccgagcg ttccttcggc ggttcccttc
    15841 ggtgcgcgcg ctctcggcgg ctcccctgga tgaggtgctg cgcgcgtggt ccgggctcgg
    15901 ctacgaccgt cgggccgtct ggctgcaccg ggcggcgcgg gcgatcgtgg atgaacatgg
    15961 ggggcgcctc ccgcgggacg tcgaagcgct ccgcgccctg cccggcgtgg ggtcctacac
    16021 ggccgcggcg gtggcctcga tcgcgttcgg ggagcccgtg ccggcgctcg acgtcaatgt
    16081 ggggcgtgtg gtggcgcggt tccgtctggg acgtgaggtg cacgaggagg gctcggaccg
    16141 tgtgctcgaa gcggcctcgg cctggatcga ccgcgacgac ccgggagcct ggaaccaggc
    16201 gctgatggat ctggggcgcg aggtgtgccg gccgacgccc cgctgcgccg cgtgcccgct
    16261 ggccccggga tgcgcgttcc ggcgcgcggg gcgtcggccg gcacgtgcgc ctcgacggca
    16321 ggatcggttc gaggggtcgt tccgccaggt ccgggggagg gtgctggccg tgctccggga
    16381 gcgtgggtcg gcctcgctgg ggtcgctgga ggccgagatc ggcgacggtc gcgtggcccg
    16441 ggccgtgcgg gcgctcgccg atgacgggct ggtctcggcc ggtcccgccg cgctggcggg
    16501 cagggcgtcg ggccgggtcc gtctggggtc catccggtga gcgacctcct cgtccgggcg
    16561 gcccgggtcc acggcgcgcc cggggcggac gccgtggcga tctcgggcca ccggatcgat
    16621 gcggtgggac cctgggacga gatccgctgg ctcggcggcg agaccgtgga ggtacccgcc
    16681 gggctgatcc tccccgggtt ccaggacgcc cacatccacg tggccgaggg gggccgcatc
    16741 ctctcgctgt gcgacctcca cgacgtgtcc gggctcgccg cctacctcga ggcggtggtg
    16801 acctacggac gccggcatcc ggaccgcccg tggatcgagg ggggcggatg gacgatgagc
    16861 gacttcccgg gagggacgcc gcaccgcgag ccgctggacg ccgccgttcc cgatcgcccc
    16921 gtcctcctgg agagccgcga cggccacgac gcgtgggtga actcgagggc gctggaggcg
    16981 gcggggatcg accggcggac gcccgacccg ccgaggggcc ggatcggccg tgacccggac
    17041 ggagagacca gcggcgtcct ccacgaggcg gccgtgcggc tggtggccga cctcctcccc
    17101 gagccctcgg ggcgggagtg ggaggaggcg attctggccg gccagggcca cctactcgcg
    17161 ctgggcgtca ccgcgtggca ggacgtgaac gtcttcccgg agtgggtgga ggcctaccgg
    17221 cggctcgccg acgacggtcg cctgatcggg cgggccacga tcgggatgca ctgggacccg
    17281 gcgcgcggcc tggagcagat cgccgagctc gtgcagcggc ggggctcctc gggccggctc
    17341 cgtgtcgaga ccgtgaagct cttccaggac ggcatcgtgg agaacggcag cgcatcgatg
    17401 ctcgagccct acacggggag cgagagccga ggcgtcagca acttcgagcc cgaggagctg
    17461 aacgagatcg tgtcggccct ggacgccgag ggcttccagg tgcacgtcca cgcgatcggg
    17521 gaccgggcgg tgcgcgaggc gcttgatgcc ttcgaggtgg cgcgcgtcgc gaacggagtc
    17581 cgcgacgcgc gccacacgat cgcccacatc caggtcgtgc acccggacga cgtcccgcgc
    17641 ttcgcggagc tcgacgtgat cccgaacggc cagccctact gggcctgcct cgagccccag
    17701 atggtcgagc tgaccctccc ggtcctcggc cccgagcgct cggcccgcca gtacccgttc
    17761 gggagcctcc ttcggacagg cgcgcgcctg gccttcggga gcgactggac ggtctcgacg
    17821 gccgaccctc tggccgagat ggcggtggcc gtgacgagac agcctcccga tgagccagaa
    17881 gcggaggtcc tcctccccga ggagcggctc acccccgggg aggcgatcga ggcctttacg
    17941 atcgggtcgg cccgcgtgaa ccgcctggag gacgtcacgg ccgcggtcga gcccggcagg
    18001 ctcgccgacc tcgtcgtcgt ggaccgcgac atcttggagc cggggtcgat ggagggagcg
    18061 agcgccgcgg tcacgatcgt cggcggccgc atcgtccacg ggccgtgacc gtcagtcggc
    18121 gacgagcgtc tggctctgct ctcgcatgaa ctgctggacg tagtagtcgc cgccgccggc
    18181 cttcccgctc gtccccgagc ccttccatcc cccgaacggc tggacgccgg gccacgcgcc
    18241 ggtcgtcgcg ccggcccgcc ggttgacgta cacgaccccc gcctcgatcc cggcgaggaa
    18301 ccgatcgatc tcgccccggt cctcgctgaa gaggccggcc gtcagcccgt actcggtgtc
    18361 gttggcgagc tcgagcgcct cgtccagcga tcccaccgcg tccaccgcga tgaacgggac
    18421 gaacagctcc cgcttccaca gccagctgtc gcggggcgcc gtcacgatcg tgggctccac
    18481 gaagttcccg cgctccaggt cgccctccgt gagccgctcg ccgccgtgga ggacctcgcc
    18541 gttcgccctc gcctccgcca ccgcctcctc gtacctggcc accgccttct cgtcgatcac
    18601 gggccccagc cagacgtctc gctccagcgg atcgccgatc cggatctcgg aggtcttctc
    18661 cagcagcgcg ctcacgagct cgtcgcgaac cggcgcctcg acgtagacgc gtgaggccgc
    18721 cgagcacttc tgccctccga acccgaacgc cgagcgcttg atcccctcgg ccgccttctc
    18781 caggtcggcc ttggccgtca cgatcaccgg gttctttccg cccatctcgc agatcacggg
    18841 gcgggggtag tcctgggtga acgacttgta gatgcccatc ccgacctcgt aggagccggt
    18901 gaaggtgatg ccgcccagcc gcgggtcggc gaccagggcg gcgccggcga cgcttccctt
    18961 cccgggcagc acctggaagg cggccggcgg gacgcccgcg tcgagcaagg cctctccgag
    19021 cttcagtccg gtgaagtagc cttggcccga gggcttcagc accactgtgt tgccggctac
    19081 cagcgcggcg ccgctcgggc cgccggccag cgccatgggg aagttgaacg ggctgatcac
    19141 gccccagacc ccatagggcc gcatcacatc gcgggtcttc tcggcctcgg acagctgccc
    19201 catcggcttg gcgaatccgt cgttccgttc catctcgtgg cagtagtagc ggatcaggtc
    19261 ggcggtctcc tcgacgtcgc ccagggcctc gaggcgggcc ttgcccacct cgatcgccat
    19321 cagggcggac agctcgttcc tccgctcgga gatcaggtcg gccgccttcc gcaggagcgc
    19381 caccctctcg tcccacgggg tcgctgccca ctcaggggcg aaggtctggg cggccgaaat
    19441 cgcgtcgtcc acgtcctgct tggacgcctc ggcgaacgtc ccgatcacca cgtcgcggtc
    19501 gatcggggag cgcgtctcct gccaggactc tccctcgcgc ggctcgccgt tgacgaggaa
    19561 cggatgctgg ccacccagcc acgatcgcgc ctcctcgatg ccgcgctcgt acgcctcgtg
    19621 gagcgcgtcg tcgtccgccg acatcgtcgc gtacgtcacc cggaaccccg cctcgcccat
    19681 agctacgcct ccttgccgtg ctcgtagtac tcgtaggcgg acatcggcct ctcgtagtcc
    19741 acccgccatt ctcgatcgtc ctccggaagc tttcccagcg ccagggccat ggcacgcacg
    19801 tgggctgcga ccgagccgca acacgagccg atcaggttca cgccgaggtc acttgcctcc
    19861 accgcccagg ctgccatctc tgaccgcggg agctggagcg ggtccagccc ctcagggaac
    19921 tccgggagcg aggtgaagtc ggggctctcg gccggcgtcc ggtaggcgac cggctggccg
    19981 gcgatccacc catccaccgc ctcccgcatc tcgcggagga tcggcagcgt gtgccccggc
    20041 ggccggaggc agttcacgcc caccacgtgg gcccccgcgt cgcggagctt tcgcgcgcag
    20101 tcggccggcg agtctccctc g
//
LOCUS       DASUTZ010000171        14637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1087985, whole genome shotgun sequence.
ACCESSION   DASUTZ010000171 DASUTZ010000000
VERSION     DASUTZ010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14637
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087985"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1629
                     /locus_tag="VF097_07635"
     CDS             <1..1629
                     /locus_tag="VF097_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6262702.1"
                     /translation="HRRAADEDRRIIVSRRGGAAMTENGFRVTYATMSADDDALHEAY
                     ERGIEEARSWLGGRHPFLVNGEPREGEGWRETRSPIDRDVVIGTFAEASKQDMDDAIA
                     TAQGFAPEWATTPWEERVRLLRKAADLISERRSELSALMAIEVGKNRLEALGDVEETA
                     DLIRYYCHEMERNDGFAKPMGQLSEAEKTRDVMRPYGVWGVISPFNFPMALAGGPSGA
                     ALVAGNTLVLKPSGQGYFTGLKLGEALLDAGIPPAAFQVLPGKGSVAGAALVADPRLG
                     GITFTGSYEVGMGIYKSFTQDYPRPVICEMGGKNPVIVTAKADLEKAAEGIKRSAFGF
                     GGQKCSAASRLYVEAPVRDELVSSLIEKTSEIRIGDPLERDVWLGPVIDEKAVARYAE
                     AVAEARANGEVLHGGERLTEGDLARGNFVQPAIVTAPRDSWLWKRELFVPFIAVDAVE
                     SLDEAVALANDTEYGLTAGLFSEDQGEIDRFLDGIEAGVVYVNRRAGATTGAWPGVQP
                     FGGWKGSGTSGKAGGGDYYVQQFMREQSQTLVAD"
     gene            complement(1633..3198)
                     /locus_tag="VF097_07640"
     CDS             complement(1633..3198)
                     /locus_tag="VF097_07640"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003995715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX6262703.1"
                     /translation="MSDFLVRASRVHGVAGADAVRVSRDRVEAVGPWEDISSLGGEAV
                     EVPGGLILPGFHDAHVHVAEGGREMSLCALHDLSGIDEYVEAVASYGHRHPERPWIEG
                     GGWTMSDFPGGMPHRDLLDDVVPDRPVYLTSRDGHDAWVNTRALEAAAIHRDTADPPR
                     GRIGRDDRGEPTGVLHEAAMDLVADLLPEASGADWEEAILAGEDHLLRLGVTAWQDVN
                     VFPGWVEAYRRLAAEGRLKGRASIGMHWDPGRGLEQIPELVERRGSSGRLRVETVKIF
                     QDGIVENGSASMLEPYTGTENRGVSNVEPQELIEIVSALDSEGFQVHIHAIGDRAVRE
                     ALDAFQAARQANGSRDARHTIAHIQIVHPDDVPRFAALGVIPNGQPFWACLEPQMVEL
                     TLPVLGPKRSAQQYPFGSLLRAGARLAFGSDWTVSTADPFEEMAVAVARRPPGEPDAE
                     VFLPDERVTPEEAIEAFTTGAARVTGFHGATIEPGRPADLVVADRDVLEPGAMEEARA
                     AVTIVGGRVVHGP"
     gene            complement(3195..4058)
                     /locus_tag="VF097_07645"
     CDS             complement(3195..4058)
                     /locus_tag="VF097_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX6262704.1"
                     /translation="MNEVLRAWYASRAGAYPWRSRPTPYRVLVSEVMLQQTRASRVAP
                     AFRAFLGRFPSVRALSAAPLDEALRAWSGLGYDRRAVWLHRAARTIVHEHGGRLPRDV
                     EALRSLPGVGSYTAAAVASIAFGEPVPALDVNVERVVARCRLGREAHEQAPGRVRAAA
                     ERWIDREDPGSWNQALMDLGREVCRPTPRCAACPLAPGCAFRRAGRRPAPPPRRQGRF
                     EGSFRQVRGRVLAVMRERGVATLATLESTIGDPRVGRAVGALADDGLVSAGPAARAGR
                     PGGRVRLGSGR"
     gene            complement(4094..4441)
                     /locus_tag="VF097_07650"
     CDS             complement(4094..4441)
                     /locus_tag="VF097_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262705.1"
                     /translation="MREIEEIRGRLSSDIDELQSRMPAAAVWGKRAVGALVGGGAAAT
                     VALLLLRRRRAKRRRQAPVQAVVNVLPEDVARRVADKLDDERVKRMLMVAGGAWLLFK
                     LAELRQLRRMRTA"
     gene            complement(4438..5688)
                     /locus_tag="VF097_07655"
     CDS             complement(4438..5688)
                     /locus_tag="VF097_07655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEX6262706.1"
                     /translation="MAPRFVSFARDVLERSGRDRSGYLAATVTYYGFLSLFPLLLLGL
                     SVIGFVLADRPNLQEEIVDAVAGAVPGLQDVMGESLDSLVDARAASGLIGLAGLVWSG
                     LGATEAAGFAVSRIFRVHPYASFVKRKLWSLWTTIVLGLMAVTALGIVALVGNLPVSG
                     WAAVSLRAGGLVVAFVLDVALFLVGYRLLTQRQGPPFGALWRGAIFAAVAWGVVKVVG
                     TWYVAGTVSRSSAVYGTLASAVGILLLIYAASQLLLYGAEVNAVALERAGKDLGSVER
                     GIYVEGDGEMRRTVNGERSTGDLVRSIATDTSQLVRKQIELAKQEMAEGAKAKAVGGA
                     ALGAAAIFGLIAFVFLGVTMAAALRIVWPAWLAWIVTAATFLLLAGVIAGVGVSRMKR
                     PVVPEETKRTVKEDVEWAKAQLRR"
     gene            5766..6749
                     /locus_tag="VF097_07660"
     CDS             5766..6749
                     /locus_tag="VF097_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020646777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6262707.1"
                     /translation="MPRTIVITGASAGVGRATARMFAAREQAAIGLLARGEQGLEAAS
                     REVETEGGTPLALPTDVADAEAVERAAAAVERHLGPIDVWVNNAMTSVFAPFTEIVPE
                     EFRRVTDVTYLGVVHGTLAALRRMGPRGRGVIVQVGSALAYRGIPLQSAYCGSKHAIQ
                     GFTESVRTELLHEGSGVHLTMVQLPALNTPQFGWVEARLPNEPQPVPPIYQPEVAARA
                     VVFAADHPRRQVWVGGSTVATILANRLVPGLLDRYLGRTGYGSQQTDETLEPGRPSNL
                     WQPVPGDQGAHGSFDDRAKDRSIQLWLATHRGAVVAAVAAGTLALVRRALR"
     gene            complement(6818..6976)
                     /locus_tag="VF097_07665"
     CDS             complement(6818..6976)
                     /locus_tag="VF097_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262708.1"
                     /translation="MPRCPVCNSIRVVMVLNGDSRAFCGRCGARWVQSGGRQTDVRRR
                     DAPADHPA"
     gene            complement(7208..7804)
                     /locus_tag="VF097_07670"
     CDS             complement(7208..7804)
                     /locus_tag="VF097_07670"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX6262709.1"
                     /translation="MMDRLKDRLASDERLAGLRDRYGYLQKELIRGLPGERRRRERRA
                     LVMGFLGGMVVGGALTYLLDPVLGRTRRARLADQAVARAREAARDTERFGRKVASDVE
                     GRIQATRRGTEDYVAPNDVTLARKVESEVLGRPDVPKGSVLVNVEHGRVVLRGTVERP
                     EVRDELTRLVLDVDGVDEVENLVQVPGHDAEGVRRLAR"
     gene            complement(7862..8380)
                     /locus_tag="VF097_07675"
     CDS             complement(7862..8380)
                     /locus_tag="VF097_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262710.1"
                     /translation="MPQKLVATFSDQERARRAALALERRGIEGSSVSLESAGREGPPV
                     EAGDTRAGDRRLSRDVGARVGVGAVIGTIAGGILGLALGLLAFPGAGVWASTIGAAIA
                     GGAVGGIIGGVSGLGTGGAWQRTYAEIREERVVVGVTHSDPDILDRAEQALRELEPDR
                     LERLDDEQRRAS"
     gene            complement(8410..8802)
                     /locus_tag="VF097_07680"
     CDS             complement(8410..8802)
                     /locus_tag="VF097_07680"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6262711.1"
                     /translation="MLDAQDTDVRILLAPSPESPAAARDAMDRFEGWVDEETLENLRL
                     VASELVTNSYRHASMRGDERVEVRAGLRSGVLRLEVRDPGGGFAAPDDPVPQAESGWG
                     LYLVDHIADRWGVNRGRDTEVWAELDVD"
     gene            complement(8832..9224)
                     /locus_tag="VF097_07685"
     CDS             complement(8832..9224)
                     /locus_tag="VF097_07685"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6262712.1"
                     /translation="MVETRSTLRIPARAAYVGTARIFAAAVARQSGVGEEHVDDLKLA
                     ISEACAAGVREDGDGAIVVHAVLDGSALSFEVSGQDIPPPPSDDFVSTPESFRRSVGY
                     DVIRSLYPDASFPSKGSAAIRFSISNAS"
     gene            complement(9228..9560)
                     /locus_tag="VF097_07690"
     CDS             complement(9228..9560)
                     /locus_tag="VF097_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007264942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6262713.1"
                     /translation="MDLTLEVRTENDTPILEVGGEVDIYTAPKLREKLVELIDGGSDR
                     IVVDLEQVGFMDSTGLGSLVAGLKRIRERDGELAIVCTREPILKVLAITGLDRVFPVH
                     DSVGNALG"
     gene            9606..9992
                     /locus_tag="VF097_07695"
     CDS             9606..9992
                     /locus_tag="VF097_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262714.1"
                     /translation="MSDRISTPVVVDVPAAPEQAYVLRSVVASVGARLDLPLDSIEDL
                     KIAVDEAVAHLLTTGGTRLRMEIFPSESGLKVTTSTDASQEAWPPEGFEGSLTWQVLT
                     GLTDEAWFAVDGGPILGFTKQALVHG"
     gene            9985..10725
                     /locus_tag="VF097_07700"
     CDS             9985..10725
                     /locus_tag="VF097_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017581906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HEX6262715.1"
                     /translation="MADESRETELFQRRDEDPAAREQLVEQFRALAEYLARRFQGRGE
                     PVEDLTQVAMIGLLKAIDRFDLGREVKFSTYATATIVGELKRHFRDKGWSVRVPRRLQ
                     ETGLKVSKAMAEVSQEVGRSPTVAEIASHTGLSEEEVLEGMETVHAYSASSLDAPIDE
                     EGRSSHELMGSEDEQIEMLEAWTTVAPAIQALPERERRILYLRFFRGLTQTQIADEIG
                     ISQMHVSRLLARTIRQLREEVRADEPVE"
     gene            complement(10729..13662)
                     /locus_tag="VF097_07705"
     CDS             complement(10729..13662)
                     /locus_tag="VF097_07705"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6262716.1"
                     /translation="MSGVHEGLEARLFQHLGAAAVAVDREGVVTLWNEHAETLFGVPR
                     EDAVGHDVIKALHEDELSEVVHALTSASGLETEVVLRDPLGADRLCFVRVARMPQPGG
                     GAIALAVDISHRRLVEQRLEAQYAAARVLAEATDVEKATRRFLREVGQAIGWDAGDVW
                     IVDADTQVMRFADLWAGPGVEGERLAEAGRTHAFTAGEGLPGRVLASGSPVWVPDVGE
                     DDNFPRKRMVMEAGLRAGFSFPIRLGDRVLGAMEFFHRDPRLPDEALLEAMDAVGVQF
                     GQLLERATALEAMQESEALKASILKASLEAVIVIDHRGTVIDWNPAAERYFGFSRHEA
                     LGWEMAEMIVPEDARERMRAGLRRFLETGESRIMGKRLEMVAQRRGGARFPVEVTVVR
                     TDLPGPPTFTGYVRDITERKQTEAALVEGERRLRHALEAGEMGAWEWDLGSGRIEWSA
                     SLERIHGLEEGSFAGTLEAFEADIYPDDRERVREAVDRALSGLNPYRIEYRIVRPDGS
                     QRWLEAIGDVRRDASGAPVRMHGVCMDVTDRREAEEIRTRLYEGEQAARAEAEAARAR
                     LSFLAEASRILSSSLNLERTLAKLAELVVPRLADWCSVELVEEGGTLKHVALGARDEE
                     IRQMVRGMRERYPPDPEGPKGIGEVLGTGEAIIYPEIPDDLVAAAAQDEEHRRTLERL
                     DIRSGMIVPLRARGRTLGAMTFASSERRFDEADLALAQELADRAAVAIDNARLYEERS
                     AIARSLQRSLLPPALPEIPGVDVAARYEPAGEGFQVGGDFYDVFQRRAGEWAIAIGDV
                     CGKGPEAAAVTGLARHTLHAAWLGDHTPSGSLRLLNEALLKSGTDRFCTVAKGRITPG
                     QGGATLTVACGGHHRPYVLRAGGGIEVVGEPGTLLGVFQDVTITDQTVRLGPGDLVIF
                     YTDGVVDDRGGPADRAPRTLMDFLEGVPRDSAGAVADHIEEQVRKANPDGPQDDVAIV
                     VLRVE"
     gene            complement(13678..14202)
                     /locus_tag="VF097_07710"
     CDS             complement(13678..14202)
                     /locus_tag="VF097_07710"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HEX6262717.1"
                     /translation="MSGGPERDAETEKERLSRKLGELLEELRVALPGVQVLFAFLLTL
                     PFTNRFSGISDLSRDVYFGSFLAAALASVFLIAPSAYHRMRWRFTDREELEDKRKMLI
                     TSSRMATTGIVLLGAAIAGVTFVIANVLFNVPVALTVSAAITGVAVVLWFALPLTRRA
                     RARQGRGPGLSAGS"
     gene            complement(14199..14342)
                     /locus_tag="VF097_07715"
     CDS             complement(14199..14342)
                     /locus_tag="VF097_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3096 domain-containing protein"
                     /protein_id="HEX6262718.1"
                     /translation="MSVNLTLPGILALAAGILVLIFPTILNYIVAIYLIVTGAILIFD
                     VRI"
     gene            complement(14444..>14637)
                     /locus_tag="VF097_07720"
     CDS             complement(14444..>14637)
                     /locus_tag="VF097_07720"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEX6262719.1"
                     /translation="LAIVIVLIVFVTQNLGEVPVDFVFVTESFPLIWVLIVTTLLGAV
                     AGYLLGRPSKAGRRERDER"
BASE COUNT         2240 a         5464 c         4954 g         1979 t
ORIGIN      
        1 caccgacgag ccgccgacga ggaccggcgg atcattgtgt ctcgcagagg aggggcggcg
       61 atgaccgaga acgggttccg ggtgacgtac gcgacgatgt cggcggacga cgacgcgctc
      121 cacgaagcgt acgagcgcgg catcgaggag gcgaggtcgt ggctcggcgg ccggcatccc
      181 ttcctcgtga acggcgagcc gcgagagggt gaggggtggc gagagacccg ctccccgatc
      241 gaccgcgacg tggtgatcgg gaccttcgcc gaggcctcca agcaggacat ggacgacgcg
      301 atcgccaccg cgcagggctt cgcccccgag tgggcgacca ccccgtggga ggagcgggtc
      361 cggctcctgc ggaaggccgc cgacctcatc tcggagcggc gcagcgagct ctccgccctc
      421 atggcgatcg aggtgggcaa gaaccgcctc gaggcactag gcgacgtcga ggagaccgcc
      481 gacctgatcc gctactactg ccacgagatg gagcggaacg acgggttcgc caagccgatg
      541 gggcagctct ccgaggccga gaagacgcgc gacgtgatgc gaccctacgg cgtctggggc
      601 gtgatcagcc cgttcaactt ccccatggcg ctggccggcg gcccgagcgg cgccgccctc
      661 gtcgcgggca acaccctcgt gctgaagccg tccggccagg gctacttcac cgggctgaag
      721 ctcggcgagg cgctgctcga tgccggcatc ccgccggccg ccttccaggt gctgcccggg
      781 aaggggagcg tggccggggc cgccctggtc gccgacccac ggctgggcgg catcaccttc
      841 accggctcgt acgaggtcgg catggggatc tacaagtcgt tcacccagga ctacccgcgc
      901 cccgtgatct gcgagatggg cgggaagaac ccggtgatcg taacggccaa ggccgacctg
      961 gagaaggcgg ccgaggggat caagcgctcg gccttcgggt tcggggggca gaagtgctcg
     1021 gcggcctcgc gcctctacgt ggaggcgccg gtccgcgacg aactcgtgag ctcgctgatc
     1081 gagaagacct ccgagatccg catcggagac ccgctggagc gagacgtctg gctcggtccc
     1141 gtgatcgacg agaaggccgt ggccagatac gcggaggcgg tggccgaggc gagggcgaac
     1201 ggcgaggtcc tccatggggg cgagcggctc acggaaggcg acctggcgcg agggaacttc
     1261 gttcagcccg ccatcgtgac cgctccgcgc gacagctggc tgtggaagcg cgagcttttc
     1321 gtgccgttca tcgcggtgga tgcggtggag tcgctcgacg aggccgtcgc cctggccaac
     1381 gacaccgagt acgggctgac ggctgggctc ttcagcgagg accagggcga gatcgaccgc
     1441 ttcctggacg ggatcgaggc cggggtcgtc tacgtgaacc gccgcgccgg cgcgaccacc
     1501 ggcgcctggc ccggcgtcca gccgttcggc ggctggaagg ggtcgggcac gagcgggaag
     1561 gccggcggcg gcgactacta cgtccagcag ttcatgcggg agcagagcca gacgctggtg
     1621 gcggactgag gatcaggggc cgtggacgac gcggcccccg acgatcgtga cggcggcccg
     1681 ggcctcctcc atcgctcccg gctcgaggac gtcccggtcg gccacgacga ggtcggcggg
     1741 gcgtcccggc tcgatcgttg cgccgtggaa acccgtgacc cgagcggcgc cggtcgtgaa
     1801 ggcctcgatc gcctcctcgg gcgtcacgcg ctcgtcgggg aggaacacct cggcgtcggg
     1861 ctctccgggc ggacggcgcg cgaccgccac ggccatctcc tcgaacggat cggccgtcga
     1921 cacggtccag tcgctcccga acgcgagccg ggcgcccgca cggagcaggg agccgaatgg
     1981 gtactgctgc gccgaccgct tggggcccag taccggcagg gtcagctcga ccatctgggg
     2041 ctcgaggcac gcccagaagg gctgcccgtt ggggatcacg ccgagcgcgg cgaaccgagg
     2101 gacgtcgtcc gggtggacga tctggatgtg agcgatcgtg tggcgcgcgt cgcgggagcc
     2161 gttggcctga cgggcggcct ggaacgcgtc gagcgcctcg cgcaccgccc ggtcaccgat
     2221 cgcgtggatg tgcacctgga acccctccga gtccagagcc gacacgatct cgatcagctc
     2281 ctggggctcg acgttgctga ccccccggtt ctcggtcccc gtgtagggct cgagcatcga
     2341 ggcgctgccg ttctccacga tcccgtcctg gaagatcttc acggtctcga cgcggagccg
     2401 gcccgaggag ccccgccgct ccacgagctc ggggatctgc tcgaggccgc gccccgggtc
     2461 ccagtgcatc ccgatcgacg cccgcccctt caggcggccc tccgcagcca gccgtcggta
     2521 ggcctccacc cacccgggga agacgttcac gtcctgccag gccgtcaccc ccagccgcag
     2581 cagatgatcc tcccccgcga ggatcgcttc ctcccagtcc gcgcccgacg cctcgggcag
     2641 gaggtcggcc accaggtcca tggccgcctc gtggaggacc ccggtgggct ccccccggtc
     2701 gtcgcggccg atgcggcccc ggggcggatc agccgtatca cggtggatcg ccgcggcctc
     2761 gagcgccctc gtgttcaccc aggcgtcgtg gccgtcgcgg ctcgtcaggt acacgggacg
     2821 atccggaacg acgtcgtcaa ggaggtcgcg gtgcggcatc cccccgggga agtcgctcat
     2881 cgtccacccg ccaccctcga tccaggggcg ctcggggtga cggtggccgt aggaggcgac
     2941 cgcctcgacg tactcatcga tccccgagag atcgtggagg gcgcacagag acatctcccg
     3001 gcctccctcg gccacgtgga cgtgggcatc gtggaacccc ggaaggatca ggccgccggg
     3061 aacctcgacc gcctcgccgc ccagcgaact gatgtcctcc cacgggccga cggcctcgac
     3121 gcggtccctg gagaccctca cggcatcggc ccccgcgacg ccatggaccc gcgaggccct
     3181 taccagaaag tcgctcatcg acccgatccc agacggacgc ggccgccggg cctcccggcc
     3241 cgcgccgccg gccccgcaga gaccagcccg tcgtccgcaa gggcgccgac cgcccgcccc
     3301 acacggggat cgccgatcgt ggattcgagc gtcgcgagcg tcgcgacgcc tcgctcccgc
     3361 atcacggcaa gtacccggcc ccgcacctgc cggaacgagc cctcgaaccg cccctgtcgc
     3421 ctgggcggcg gtgccggccg acgccccgcg cgccggaacg cgcatcccgg ggccagcgga
     3481 catgcggcgc agcggggcgt cggccggcac acctcccgcc ccagatccat cagggcctgg
     3541 ttccacgagc ccgggtcctc ccgatcgatc cagcgctcgg ccgcggcccg cacccgtccc
     3601 ggcgcctgct cgtgcgcctc acgtcccagc cggcaccgcg ccaccacacg ctccacgttc
     3661 acgtcgagcg ccggcacggg ctccccgaac gcgatcgagg ccaccgccgc ggccgtatac
     3721 gaccccacgc cgggcagaga acggagcgct tcgacgtccc tcgggaggcg cccgccgtgc
     3781 tcgtggacga tcgtccgtgc cgcccggtgc agccagacgg cccgacggtc gtagccgagc
     3841 ccggaccacg cgcgcagcgc ctcgtccagg ggagccgccg acagcgcacg caccgatggg
     3901 aaccgcccca agaaggcccg gaaggcgggc gccaccctcg aggcccgcgt ctgctggagc
     3961 atcacctcgg agacgagcac gcggtacggg gtcggacggg accgccacgg gtaagccccc
     4021 gctctcgacg cgtaccaggc cctcagcacc tcgttcacgc gcctaccttc gcatggagga
     4081 ggcgccgcct gactcaggct gtgcgcatgc ggcggagctg gcggagctcg gcgagcttga
     4141 acagcagcca ggctcctccc gccaccatca gcatccgctt cacccgctcg tcgtcgagct
     4201 tgtccgccac cctcctggcg acgtcctcgg ggaggacgtt gaccaccgcc tggacggggg
     4261 cctgacgccg ccgcttggcc cgccgccgcc gcaggagcag gagcgcgacg gtcgccgcgg
     4321 ccccgccccc gacgagggcg cccacggccc gcttgcccca gacggcggcc gcgggcatgc
     4381 ggctctggag ctcgtcgatg tccgaggaga gccgcccgcg gatctcctcg atctccctca
     4441 ccgtctcagc tgcgctttgg cccattccac atcctccttc accgtgcgct tcgtctcctc
     4501 gggcaccacg ggccgcttca tccggctcac ccccaccccg gcgatcacgc cggccagcag
     4561 caagaacgtc gccgcggtca cgatccaggc cagccaggcc ggccagacga tccggagcgc
     4621 cgcggccatc gtcacgccga ggaacacgaa cgcgatcagg ccgaagatgg ctgccgcgcc
     4681 gagtgcggcc ccgcccacgg ccttcgcctt ggccccctcg gccatctcct gcttggcgag
     4741 ctcgatctgc ttccgcacca gctgcgacgt gtccgtcgcg atcgaccgca ccaggtcgcc
     4801 ggtggaccgc tctccgttca ccgtgcgtcg catctcacca tctccttcca cgtagatgcc
     4861 tcgctccacg ctcccaaggt ccttccccgc ccgctccagc gcaaccgcgt tgacctccgc
     4921 tccgtacagc agaagctggg acgcagcgta gatcagcagc aggatcccga ccgcactggc
     4981 cagcgtcccg tacaccgcgc tcgaccgcga gacggtgccg gcgacgtacc aggtgccgac
     5041 gaccttgacg accccccagg ccaccgcggc gaagatcgcc cctctccaca gggcgccgaa
     5101 gggcggcccc tgccgctggg ttaggagccg ataccccacc aggaacagcg cgacgtccag
     5161 gacgaaggcc accaccaggc cccccgcgcg caggctcacg gcggcccatc cggacaccgg
     5221 caggttcccc accagcgcca cgatcccgag ggccgtcacc gccatcagcc cgagcacgat
     5281 cgtcgtccag agcgaccaga gcttccgctt cacgaacgag gcgtaggggt gcacgcggaa
     5341 gatccggctg acggcgaatc cggcggcctc ggtggcgcca aggccggacc acaccagacc
     5401 ggccagcccg atcaggccgg acgcggcccg tgcgtccacc agcgagtcca ggctctcgcc
     5461 catgacatcc tgcaggcccg gcaccgcgcc ggcgaccgcg tccacgatct cctcctgcag
     5521 gttcgggcgg tccgccagca cgaagccgat caccgagagg cccagcaaga gcagggggaa
     5581 gagcgagagg aacccgtagt acgtcacggt cgccgccaga tacccgctcc ggtcgcgacc
     5641 ggaccgctcc agcacgtcgc gggcgaagga gacgaaccga ggggccacgg ggggaagcct
     5701 acccgggagg cagagcgata accgagcgcg tttagggcgg ggccacccgg ggggatggaa
     5761 ccgtcgtgcc gagaacgatc gtgatcacgg gagcgtcggc cggcgtggga cgagcgacgg
     5821 cgcggatgtt cgccgcgcgc gagcaggccg cgatcggcct gctcgcgcgc ggcgaacaag
     5881 gcctcgaggc cgcctccagg gaggtcgaga ccgagggcgg aacccctctc gccctcccca
     5941 ccgacgtcgc cgacgccgag gccgtggagc gggcggcggc ggcggtcgag cggcatctgg
     6001 gaccgatcga cgtctgggtc aacaacgcca tgacgtcggt cttcgcccct ttcaccgaga
     6061 tcgtgcccga ggagttccgc cgcgtcaccg acgtgaccta cctgggcgtg gtccacggca
     6121 ccctggccgc gctccgccgc atggggcccc gcggccgggg ggtcatcgtc caggtcggct
     6181 cggcactcgc ctaccggggg atccccctcc agtcggccta ctgcggctcg aagcacgcca
     6241 tccaggggtt cacagagtcc gtccgaaccg agctcctcca cgaggggagc ggcgttcacc
     6301 tcacgatggt ccagctgcct gcgctcaaca ccccccagtt cggctgggtg gaggcacgcc
     6361 tcccgaacga gccccagccc gtgccgccca tctaccagcc cgaggtggcg gcgcgggccg
     6421 tcgtcttcgc cgccgatcac ccgaggcgtc aggtctgggt cgggggctcc accgtggcca
     6481 cgatcctcgc gaaccggctg gtccccggcc tcctcgatcg gtacctgggg aggaccggct
     6541 acggctcgca gcagaccgac gagacgctgg agccggggcg cccctcgaac ctctggcagc
     6601 ccgtcccggg agaccagggc gcacacggct cattcgacga ccgcgccaag gaccgcagca
     6661 tccagctttg gctcgccacc catcgaggag cggtggtcgc ggccgtcgcc gcgggcaccc
     6721 tcgcgctcgt ccgccgggcg ctccgctgag tcagaggaac cgggccaact tcgcaaggtc
     6781 ttccacgagc gtacggctcg gtcctctcgg ccaccactca ggccggatgg tcggccggcg
     6841 cgtcccttcg gcggacgtcg gtctgacggc cgccgctctg cacccaccgc gcgccgcagc
     6901 ggccgcagaa cgcccggctg tcaccgttca ggaccatcac gacacggatc gagttgcaga
     6961 cgggacagcg cggcatcaag gtcatcctcc tcggctccgg ccccccgaga ggggcatcca
     7021 gggtcgtgcg ttcattccct caccccccgc gcccgaaacg cctcctcgaa ctcccgaaaa
     7081 aaaatcgggg agaaggtggt ttgggggccg gatccgtggg aaagctggag gcacaaccga
     7141 ccgcgggaga cctccctctc tcgacgacaa ggggccgcgc tcagcgcggc ccctttcgtg
     7201 tccgggctca cctcgccagg cgccgaaccc cttccgcgtc atggccgggc acctgcacaa
     7261 ggttctccac ctcgtccacc ccgtccacgt ccagtaccag gcgggtcagc tcgtcccgca
     7321 cctcgggccg ctcgacggtg cccctcagga ccacccgccc gtgctccacg ttgacgagca
     7381 ccgacccctt gggcacgtcc gggcgcccga ggacctcgct ctccactttg cgagcgagtg
     7441 tgacgtcgtt gggcgcgacg tagtcctcgg tcccgcggcg cgtggcctgg atgcgcccct
     7501 ccacgtcgga ggcgaccttc cggccgaagc gctccgtgtc cctcgccgcc tcgcgagccc
     7561 gggccacggc ctggtcggcc agcctggccc gacgcgtgcg gccgagcacc ggatccagca
     7621 ggtacgtgag cgctccgccc acgaccatgc ctcccaggaa gcccatcacc agagcgcgtc
     7681 gctctctcct gcgacgctcc ccggggagcc cgcggatcag ctccttctgc agatatccgt
     7741 agcggtcgcg caggcccgcc agccgctcat cggatgccag tcgatccttg agacgatcca
     7801 tcatcggcag tcacctccgt ccgatgggta cccgggccgg atgcggacaa acaagaagga
     7861 gctaagacgc ccggcgctgc tcgtcgtcca gccgctcgag ccggtccggc tccagttctc
     7921 ggagggcctg ctcggcccgg tcgaggatgt cggggtcgga atgcgtgaca ccgaccacga
     7981 cgcgttcctc tcggatctcg gcgtaggttc gctgccacgc acctccggtc ccgaggcccg
     8041 agacgcctcc gatgatcccg ccgacggccc ctcccgcgat ggcggccccg atggtggagg
     8101 cccagacacc tgcccccggg aaggccagga gacccagggc gagcccgagg atccctcctg
     8161 cgatcgttcc gatcaccgcc ccgaccccga cccgtgctcc gacgtcccgg ctgagacgac
     8221 gatcgcccgc gcgagtgtcg ccggcctcga cgggagggcc ctcacggccc gcgctctcga
     8281 gcgagacgct ggatccctcg atcccccgcc gctccagagc gagggcggct cggcgggcgc
     8341 gctcctgatc ggagaaggtc gctacgagct tctgaggcat ggcggatccc tacccgatcg
     8401 gcgggcagct cagtccacgt cgagctccgc ccagacctcg gtgtcccgcc cccggttcac
     8461 accccagcgg tccgcgatgt ggtccacgag gtacagcccc cacccggact cggcctgggg
     8521 cacggggtcg tctggcgccg cgaacccgcc cccgggatcc cggacctcga gccggagcac
     8581 gccgctgcgc aggccggcac gaacctcgac gcgctcatcg cctcgcatcg acgcgtgccg
     8641 gtagctgttc gtcaccagct cgctcgcgac gagccggagg ttctccaggg tctcctcgtc
     8701 gacccacccc tcgaatcgat ccatcgcgtc gcgcgcagct gcgggggact cgggggacgg
     8761 ggcgaggagg atcctcacgt ccgtgtcctg cgcgtccaac atcacctgac tattgatacc
     8821 ccgtctcagg ctcacgacgc gttcgagatc gaaaaccgga tggctgccga gcccttcgac
     8881 gggaaggagg cgtccgggta gagagaccgg atcacgtcgt acccaacgct gcgacggaag
     8941 gactcgggtg tggacacgaa atcgtccgaa gggggaggag ggatgtcctg gccggagacc
     9001 tcgaaggaca gcgccgatcc gtcgaggacg gcgtggacca cgatcgcccc gtccccgtcc
     9061 tcacggaccc cggcggcgca cgcctccgag atggcgagct tgaggtcgtc aacgtgctcc
     9121 tcgcccacgc cggactgccg ggccaccgcc gcggcgaaga tccgggccgt gcccacgtag
     9181 gcagctcggg cggggatccg cagcgtggaa cgggtctcga ccaacgctca gcccagcgcg
     9241 ttcccgacgg agtcgtgcac ggggaacacg cggtcgaggc cggtgatcgc aaggaccttc
     9301 aggatcggct cccgggtgca gacgatcgcg agctcgccgt cgcgctcccg gatccgcttc
     9361 aggccggcga cgaggctgcc caggccggtg gagtccatga agcccacctg ctcgaggtcc
     9421 accacgatcc ggtcgctgcc cccgtcgatc agctcgacga gcttctcccg gagcttcggg
     9481 gcagtgtaga tgtcgacctc gcctcccacc tcaaggatgg gggtgtcgtt ctcggttcgc
     9541 acttcgaggg tcaggtcgat ggctcctcac ctcgtcgcat cggcggcctg tagtataggc
     9601 ggtccgtgag cgatcgtatc tcgacccccg tcgtcgtcga cgtccctgcc gcccccgagc
     9661 aggcctatgt gctgcgaagc gtcgtggctt cggtgggcgc gcgtctcgac ctgccgctcg
     9721 acagcatcga ggacctcaag atcgccgtgg acgaggcggt ggcccatctg ctgacgaccg
     9781 gcgggacgcg gctccgcatg gagatcttcc cctcggaatc gggcctcaag gtgaccacgt
     9841 cgaccgacgc ctcccaggag gcctggcccc ccgagggctt cgagggatcg ctcacctggc
     9901 aggtgctgac cggcctgacc gacgaggcgt ggttcgccgt cgacgggggg ccgatcctcg
     9961 gcttcacgaa gcaagccctc gtccatggct gacgagtccc gcgagaccga gctgttccag
    10021 cggcgggacg aggaccccgc cgccagggag cagctcgtcg agcagttccg cgcgctggcc
    10081 gagtacctgg cccggcgctt ccagggtcgg ggcgagccgg tcgaggacct gacccaggtg
    10141 gcgatgatcg gactgttgaa ggcgatcgac cgcttcgacc tcgggcgcga ggtgaagttc
    10201 tcgacctacg ccacggccac gatcgtcggg gagctgaagc gccacttccg cgacaagggg
    10261 tggtccgttc gcgtcccgcg ccgcctgcag gagaccggcc tcaaggtcag caaggcgatg
    10321 gctgaggtct cgcaggaggt cggacgctcg cccaccgtgg ccgagatcgc ctcccacacg
    10381 gggctctccg aggaagaggt cctcgagggc atggagaccg tccacgccta ctccgcctcg
    10441 tcgctggacg ccccgatcga cgaggagggc cgcagctcgc acgagctgat gggctccgag
    10501 gacgagcaga tcgagatgct cgaggcctgg accaccgtgg ccccggcgat ccaggcgctc
    10561 cccgagcgag agcggaggat cctctacctg cggttcttcc gggggctcac gcagacccaa
    10621 atcgccgacg agatcgggat ctcccagatg cacgtgtcca ggctgctggc gcggacaatc
    10681 cgccagctcc gcgaggaggt ccgggcggac gagccggtgg agtgaccgtc actccacgcg
    10741 gaggaccacg atcgccacgt cgtcctgcgg cccgtccggg ttcgccttcc tcacctgctc
    10801 ctcgatgtgg tcggcgacgg cccccgccga gtcccgaggg acgccctcca ggaagtccat
    10861 cagcgtgcgc ggggcccggt cggccggccc gcctcgatcg tccacgacgc cgtccgtgta
    10921 gaagatcacc aggtcgccgg ggccgagccg cacggtctga tcggtgatcg tgacgtcctg
    10981 gaagacgccc aggagcgttc cgggctcgcc cacgacctcg atgccgccgc ccgcccggag
    11041 gacgtacggg cggtggtggc ccccgcaggc gaccgtcagc gtcgcgccac cctgcccggg
    11101 ggtgattcga cccttggcca ccgtgcagaa gcggtcggtc cccgacttga gcagtgcctc
    11161 gttgaggagc cggagcgacc cgctgggcgt gtggtcaccc agccaagcgg cgtggagcgt
    11221 gtgccgagcc aggccggtca cggcggccgc ctcaggcccc ttcccgcaga catcgccgat
    11281 cgcgatcgcc cactcccccg cgcggcgttg gaacacatcg tagaagtcgc ccccaacctg
    11341 gaaaccctcg ccggcgggct cgtaccgagc cgcgacgtcg accccaggga tctccgggag
    11401 ggccgggggg agcaggctgc gctgcaggct gcgggcgatc gcgctccgct cctcgtacag
    11461 ccgcgcgtta tcgatcgcga cggcggcccg atcggccagc tcctgggcca gcgcaaggtc
    11521 cgcctcatcg aaccgtcgct cgctcgaggc gaaggtcatc gcacccagcg ttcgcccgcg
    11581 agccctcagc ggcacgatca tcccggagcg gatgtccagc cgctccagcg tgcgcctgtg
    11641 ctcctcgtcc tgcgccgcgg cggccaccag atcgtccggg atctcggggt agatgatcgc
    11701 ctcgcccgtc ccgaggacct caccgatgcc cttgggaccc tcgggatcgg gcgggtagcg
    11761 ctccctcatc cctcggacca tctgccgaat ctcttcgtcg cgagccccca gggcgacgtg
    11821 cttgagggtg cccccctcct ccaccaactc cacgctgcac cagtccgcca ggcgcgggac
    11881 cacgagctcc gcgagcttgg ccagggtccg ctccaggttg agcgacgagc tcaggatgcg
    11941 gctcgcctcg gccaggaacg acaggcgagc cctcgccgcc tcggcctcgg cccgggccgc
    12001 ctgctcgccc tcgtagaggc gggtcctgat ctcctcggcc tcgcgccgat ccgtcacgtc
    12061 catgcagacc ccgtgcatcc gcacgggcgc gcccgaggcg tcgcgcctga cgtcgccgat
    12121 cgcctccagc cagcgctggg agccgtccgg ccgcacgatc cgatactcga tccggtaggg
    12181 gttgaggccc gacagcgccc ggtcgaccgc ctcccggacc cgctcgcggt catccgggta
    12241 gatgtcggcc tcgaaggcct cgagcgttcc ggcgaaggac ccctcctcga gaccgtggat
    12301 ccgctccagg ctggcggacc actcgatgcg gcccgacccc aggtcccact cccaggcgcc
    12361 catctctccg gcctccagcg cgtggcggag ccggcgctcg ccttccacca acgccgcctc
    12421 ggtctgcttc cgctcggtga tgtcccggac gtagccggtg aaggtgggag gaccggggag
    12481 gtccgtgcgc accacggtga cctcgacggg gaagcgagcg ccgccccggc gctgggccac
    12541 catctcgagc cgcttgccca tgatccgcga ctccccggtc tcaaggaagc gccgcagacc
    12601 cgctcgcatc cgctcgcggg cgtcctcggg gacgatcatc tcggccatct cccagcccag
    12661 ggcctcgtgg cgggagaatc caaagtaccg ctcggcggcg ggattccagt cgatcaccgt
    12721 cccccgatgg tcgatcacga tcacggcctc gagcgaggcc ttcaggatcg aggccttgag
    12781 tgcctcgctc tcctgcatcg cctccagcgc cgtggcccgc tccaggagct ggccgaactg
    12841 gacgcccacc gcgtccatcg cctcgaggag ggcttcgtcg ggaagacggg ggtctcggtg
    12901 gaagaactcc atggctccga gcacgcgatc cccgagcctg atcgggaacg agaagccggc
    12961 gcgcaggcca gcctccatca ccatccgctt ccgggggaag ttgtcgtcct cgcccacgtc
    13021 gggcacccag acggggctgc ccgaggccag cacgcgcccc gggagcccct cgccggccgt
    13081 gaaggcatgg gtccggccgg cctcggcgag gcgctccccc tccacgcccg gacctgccca
    13141 gagatcggcg aaccgcatga cctgcgtgtc cgcgtcgacg atccagacgt ccccggcgtc
    13201 ccatccgatc gcctggccca cctcgcggag gaacctacgg gtggccttct ccacgtccgt
    13261 cgcctccgcg aggaccctgg ccgccgcgta ctgcgcctcg aggcgctgct ccacgagtcg
    13321 ccggtggctg atgtcgacgg cgagcgcgat cgctccgccc cccggctggg gcatgcgggc
    13381 gacgcggacg aagcagagac gatcggcacc gagcgggtcc cggaggacga cctcggtctc
    13441 gaggccgctg gccgaggtga gggcgtgcac cacctcggag agctcgtcct cgtggagcgc
    13501 cttgatcacg tcgtggccca cggcgtcctc gcgggggacg ccgaacaggg tctcggcgtg
    13561 ctcgttccag agcgtcacga ccccctcgcg gtcgacagcg accgcggcgg cgccgaggtg
    13621 ctggaagagg cgggcttcga ggccttcgtg gacacccgac atgggaaagc gaggatacta
    13681 cgagcccgcg gacagccctg gccctctccc ttgcctcgcc cgggcccttc tggtgagggg
    13741 gagagcgaac cagaggacga ccgccacgcc ggtgatcgcc gcgctgaccg tcagggccac
    13801 cggcacgttg aacagcacgt tggcgatgac gaaggtcacc cctgcgatcg cggctccgag
    13861 aaggacgatg cccgtcgtgg ccatcctgct cgaagtgatc agcatcttcc gcttgtcctc
    13921 cagctcctcc ctgtcggtga agcgccaccg catccggtga taggcggacg gggcgatcaa
    13981 gaacaccgag gccagggccg cggcgaggaa ggacccgaag tagacgtccc gagagaggtc
    14041 ggagatccct gagaaccgat tcgtgaacgg gagggtcagc aggaaggcga acagcacctg
    14101 gacgccgggg agggcgacgc ggagctcctc gagcagctcg cccagctttc gggagagtcg
    14161 ctccttctcg gtctcggcgt cgcgctccgg ccccccgctc atatccggac gtcgaagatc
    14221 aggatcgccc ccgtcacgat caggtagatc gccacgatgt agttgaggat ggtggggaag
    14281 atgaggacca ggatccccgc ggccagggcc aggatccccg gcagggtgag attgacggag
    14341 atgtccccgg cctgggccac gagcgcggtc gcgattccga gcatgcttcc tcctgtgatc
    14401 gggacggccc tggcctaccc tccgcgagag cagagcaaac cgttcagcgc tcgtcccgct
    14461 cccggcgccc ggccttggat ggccgcccaa gcaggtagcc ggccaccgcg cccagaaggg
    14521 tcgtgacgat cagcacccag atcagcggga agctctcggt gacgaacacg aagtcgacgg
    14581 ggacctcgcc cagattctgg gtcacgaaga cgatcaggac gatcacgatc gccagga
//
LOCUS       DASUTZ010000172         2919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1088413, whole genome shotgun sequence.
ACCESSION   DASUTZ010000172 DASUTZ010000000
VERSION     DASUTZ010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088413"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="VF097_07725"
     CDS             complement(<1..972)
                     /locus_tag="VF097_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEX6262720.1"
                     /translation="MTHRIALIPGDGIGPEVSEAMRLVVAGAGVRVEWDVQEAGAAAI
                     EREGTPLPDRVLEAVKRSRTAIKGPVTTPVGTGFRSVNVALRRELDLFAAIRPSRHFP
                     GILTRHPGVDLVVIRENTEDLYQGVEFERGSRELAALRETLDRLAGYHLAEDAGVTVK
                     PISVTGTRRIVRAALEYARRNHRKHVLVGHKANIMKFSDGVFLATAMEVAEEYPDVEV
                     WEMQVDELAMWLAWEPEVMDVILLPNLYGDIISDLCAGLVGGLGIAPGANLGWEYALF
                     EPVHGSAPDIAGKGVANPIAMILSAALMLRHLSEDHAASRVEAAVASV"
     gene            complement(1016..1366)
                     /locus_tag="VF097_07730"
     CDS             complement(1016..1366)
                     /locus_tag="VF097_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016431403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HEX6262721.1"
                     /translation="MPQRSWSAKDERKYEHIKKSQKDQGKSSDRAEEIAARTVNKDRA
                     QEGRTKSSSRSSRRETSSSRRGGRRSGTNRPKGRTKEQLYNDAKRMGIEGRSKMTKQQ
                     LQRAVAGKKGGRRS"
     gene            complement(1449..2159)
                     /locus_tag="VF097_07735"
     CDS             complement(1449..2159)
                     /locus_tag="VF097_07735"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEX6262722.1"
                     /translation="MTETLSLEVKIRRFNPEVSAEPWWDTFTLDAEPSDRLLDALHEI
                     KWRQDGTLALRRSCAHGICGSDAMLINGHNALACKVLVRDVAPNVTVEPIRGLPVLKD
                     LIVDMEPFFAQYRSVLPYLINESGAPERERLQSQEERERFDDTTKCILCAACTTACPV
                     YWGDDGYVGPAAIVNAHRFIFDSRDEAARERLRILGEKSGVFRCRTIFNCTEDCPRGI
                     KVTKAIQEVKRAILFDKI"
     gene            complement(2160..>2919)
                     /locus_tag="VF097_07740"
     CDS             complement(2160..>2919)
                     /locus_tag="VF097_07740"
                     /inference="COORDINATES: protein motif:HMM:NF014907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX6262723.1"
                     /translation="TEFARIYLGVEPTTEPVPIQPTAHYGMGGIPTDVDGRVVVGEAE
                     EVVPGLYAAGECACVSVHGANRLGTNSLLDIVVFGRRAGRHAAAFVAEVGHAEVPPDA
                     AAATEDEVKRLLGSSGSVRMADVRADLQEAMTDLAGVVRTEEGLRKALETLGDLRERY
                     GRAFVEDKGSVYNTDLMEAWELGALLDCADTLVAAALARDESRGGHFRADHELRDDDS
                     WLRHSLAYREGDGSVRLEYKPVKMGPYVPMERKY"
BASE COUNT          396 a         1037 c         1008 g          478 t
ORIGIN      
        1 gaccgaggcc accgcggcct cgacccgcga ggcggcgtgg tcctcggaga ggtgccgcag
       61 catcagggcc gccgagagga tcatcgcgat cgggttggcc acgcccttcc cggcgatgtc
      121 gggggcgctg ccgtgcaccg gctcgaacag cgcgtactcc cagccgaggt tggcgcccgg
      181 cgcgatcccc aggccgccga cgagccccgc gcacagatcg ctgatgatgt cgccgtagag
      241 gttggggagg aggatcacgt ccatcacctc gggttcccag gccagccaca tcgcgagctc
      301 gtccacctgc atctcccaga cctcgacatc ggggtactcc tcggccacct ccatggcggt
      361 ggccaggaag acgccgtcgg agaacttcat gatgttcgcc ttgtgtccga ccaggacgtg
      421 cttccggtgg ttgcgcctcg cgtactcgag ggcggcccgg acgatccggc gggtgccggt
      481 cacgctgatc ggcttcacgg tcacgccggc gtcctcggcg aggtggtacc cggcgaggcg
      541 atcgagcgtc tctcgcagcg ccgccagctc gcgcgaccca cgctcgaact ccacgccctg
      601 gtagaggtcc tcggtgttct cccggatcac cacgaggtcc acgcccgggt gacgggtgag
      661 gatcccgggg aagtgacggg aggggcggat ggcggcgaag aggtcgagct ctcgccgcag
      721 ggccacattg acgctgcgga acccggtccc gacgggggtg gtgaccgggc ccttgatcgc
      781 ggtacgggag cgcttgacgg cctcgagcac gcggtcgggg agcggcgttc cctcccgctc
      841 gatcgcggcc gcgccggcct cctggacgtc ccactccacc cgaaccccgg ccccggccac
      901 gaccagccgc atggcctcgg agacctcggg gccgatcccg tcgccgggga taagggcgat
      961 gcggtgggtc atcggccctc aggctagcaa ggcccgaggg ccggagaccc cagggtcagc
     1021 tccgcctgcc gcccttcttg ccggcgacgg cgcgctgcag ctgctgcttc gtcatcttgg
     1081 agcggccctc gatgcccatc cgcttggcat cgttgtagag ctgctccttg gtgcggccct
     1141 tgggccggtt cgtccccgac ctgcgtccgc cccgcctcga ggagctcgtt tcgcgcctgg
     1201 aggacctgga ggacgactta gtccgcccct cctgcgcccg gtccttgttg accgtgcgcg
     1261 cggcgatctc ctcggcgcgg tcgctgctct tcccttgatc cttctggctc ttcttgatgt
     1321 gctcgtactt ccgctcgtcc ttcgcgctcc agcttcgctg cggcatgtgt atctcacctc
     1381 ctccaggggg gtacccgtcg tccccttcgt caaacggcag ggtcggcctg ggcaagaaga
     1441 ggtgcggctc agatcttgtc gaagaggata gctcgcttca cttcctggat cgccttcgtc
     1501 accttgatgc cgcgggggca gtcctcggtg cagttgaaga tggtccggca gcggaagacc
     1561 cccgacttct cgccgaggat gcggagccgc tcgcgagcgg cctcgtctcg cgagtcgaag
     1621 atgaagcggt gagcgttgac gatggcggca ggccccacgt agccgtcgtc gccccagtac
     1681 acggggcatg cggtggtgca ggcggcgcag aggatgcact tcgttgtgtc gtcgaagcgc
     1741 tcccgctcct cctgcgactg gagccgctcg cgctcgggcg cgcccgactc gttgatcagg
     1801 taggggagga ccgagcggta ttgggcgaag aacggctcca tgtccacgat gaggtccttc
     1861 aggacgggga ggccccggat cggctcgacg gtgacgttcg gggccacgtc ccggacgagg
     1921 accttgcacg ccagggcgtt gtgcccgttg atcagcatcg cgtcggagcc gcagatcccg
     1981 tgggcgcacg agcgccgcag ggcaagggtg ccgtcctgcc gccacttgat ctcgtgcagc
     2041 gcgtcgagga gccggtccga gggctccgca tcgagggtga acgtgtccca ccagggctcc
     2101 gccgacacct ccgggttgaa ccggcggatc ttgacctcca gggacagggt ctcggtcatc
     2161 tagtacttcc tctccatggg gacgtaggga cccatcttca ccggcttgta ctccagtcgg
     2221 accgagccgt ccccctcgcg gtaggcgagc gagtgccgca gccagctgtc gtcgtcacgc
     2281 agctcgtggt cggcgcggaa gtggccgccg cgcgactcgt cccgggccag ggccgcggcc
     2341 acgagcgtgt cggcgcagtc gagcagcgcg ccgagctccc aggcctccat caggtcggtg
     2401 ttgtagacgg agcccttgtc ctcgacgaag gcccggccgt accgctcgcg caggtcgccc
     2461 agggtctcca gcgccttccg caggccttcc tcggtgcgga ccacgcccgc caggtcggtc
     2521 atcgcctcct gcagatcggc ccgcacgtcg gccatccgga cggagccgct cgagccgagc
     2581 aggcgcttga cctcgtcctc ggtggcggcc gccgcgtccg gagggacctc cgcgtgcccc
     2641 acctcggcga cgaaggccgc ggcgtgcctc cccgctcgcc gcccgaacac aacgatgtcc
     2701 agcagggaat tcgtccccag gcgattcgcg ccgtggaccg agacgcaggc gcactcgccg
     2761 gcggcgtaga ggcccggcac gacctcctcg gcctccccca ccaccacgcg gccgtccacg
     2821 tcggtgggga tgccgcccat gccgtagtgc gcggtgggct ggatcggcac gggctcggtg
     2881 gtcggctcca cgccgaggta gatgcgggcg aactcggtc
//
LOCUS       DASUTZ010000173         4279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1607085, whole genome shotgun sequence.
ACCESSION   DASUTZ010000173 DASUTZ010000000
VERSION     DASUTZ010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607085"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1833
                     /locus_tag="VF097_07745"
     CDS             82..1833
                     /locus_tag="VF097_07745"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX6262724.1"
                     /translation="MAQPLPEKQCDLVMKGGITSGVVYPSAVLRLKDEYRFRSVGGTS
                     VGAIAAAIVAAAEHGRVTGRGTGFAELEAMNEELRRPGMLQSLFVPRRGGRPLYEVAS
                     AAMSKRGGLHVLRVLLRRGWWALGGLLLWWASLAWAMWFLGDRWWLVPVLLGAWAVAG
                     FVVAAMVTGRWRPAFLLASLVLALAWPAAAAPAAVVARGLAELGGKGFGIVSGREEGG
                     TEALGEWLHARIQRAAGLPDDEPLTFGDLESSEIHLRMMTTDVAAARPVRLPGELQEF
                     SFRPDDVRGILPERVASVLIERGRGLAARRGLPEGDLLPMPPTDDIPILLAFRMSLAF
                     PGLFTAVRLWSEQIQGPPRAHWFSDGGISSNFPIHFFDSWMPTRPTFGLSLGPGTGKA
                     VEPPPKANEARLPGWRRVESLGGFVKAIVDTMQEWRDTMQAELPGFRDRVWEVRLAAD
                     EGGFNIGMGPETIARLIEKGDEAGRSIREEFDWDQHVFTRYLMAMRRLELGLVGGIDP
                     AGREQRGIRAAFDARREWFERPSRVELFDHDPEWLRKAAASTERLLSEMEGWREFGGF
                     AGQSPRPPGEMRVVPDV"
     gene            complement(1838..2515)
                     /locus_tag="VF097_07750"
     CDS             complement(1838..2515)
                     /locus_tag="VF097_07750"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HEX6262725.1"
                     /translation="MSDRPGRYLEVRRNSIAIVLIAAFAVGLMLIMMGLFSWPMILPG
                     LLLILYFDGQTKDGRLLDPTSWVRGLHGERAVAKALERLPAGYRVIHDLDIGRGNVDH
                     VVIGPTGVFAIETKAWSGRLLARAGRLLHNGRDAAETRRQAVSGALAVRRHLDAAGMS
                     HTWVEAIIVSTRALVENGELELPQTKVLDVNALVPHIERSRQSLSPEQVARAADAVTR
                     PLGTLSA"
     gene            complement(2555..3721)
                     /locus_tag="VF097_07755"
     CDS             complement(2555..3721)
                     /locus_tag="VF097_07755"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HEX6262726.1"
                     /translation="MTQTQDTTPARLGLPEGARGVLPSQALERAIDQGIIDAGDYRVP
                     DEAIQPASVDLRLGETAYRIRSSFLPDKRPVEVKLKEFVVDELDLRRDGAVLETNRPY
                     LIPLIERLNLPPGVRGKANPKSSTGRLDVFTRVITDEGFRFDEIREGYAGPLYLEVVP
                     LSFTVKVQHKLALSQLRLSIGNAKLTDEEIREVHASEPLLFVEGAPAPDQDLATADGL
                     FLGLDLRGDANGRVGYRAKDYAPAIEMARTRAYEWERYWEPVYKEEGDRVVLAPEHFY
                     LLLSEEAVRIPPGFAAEMAAYDPTSGELRTHYAGFFDPGFGFDREGRFQGSRAALEVR
                     AHDVPFLIEHRQRVCKLAFERMAEEPEVLYGEAIGSSYQQQTDTLGKHFIQPPA"
     gene            complement(3767..4237)
                     /locus_tag="VF097_07760"
     CDS             complement(3767..4237)
                     /locus_tag="VF097_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017823508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX6262727.1"
                     /translation="MDQVTPALRSLERSSLDFEVVRTERPSSVEESASLQGIEVDQLL
                     RTIVVRRAEDDYVFVLVPGGREIDWPRLRKHLGVRRLSLPSVDQAREATGYERGAITP
                     LGSARPWPVIADASIPADRRVAIGGGAHGVNLHVVAGDLIEHLGADVAEVTKRA"
BASE COUNT          559 a         1502 c         1581 g          637 t
ORIGIN      
        1 ccgcccccct cctcgtttcg gggttgaccc cgtccccttc ccgttccagg ctgagcaggc
       61 cccgagcgaa ggaggtccgc catggctcag cctctgcccg agaagcagtg cgatctcgtc
      121 atgaagggcg ggatcaccag cggcgtggtc tatccctcgg ccgtcctccg gctgaaggac
      181 gagtaccggt tccggtcggt gggcggcacc tcggtcgggg cgatcgccgc cgccatcgtg
      241 gccgccgccg agcatggccg ggtcacgggc cgggggaccg ggttcgccga gctcgaggcc
      301 atgaacgagg agctccggcg gccgggcatg ctccagtcgc tcttcgtccc caggcgcggc
      361 ggacgccccc tctacgaggt cgcgtccgcc gccatgtcga agcggggcgg gctccacgtc
      421 cttcgcgtcc tgctccgccg cggctggtgg gccctgggag gcctcctcct ctggtgggcc
      481 tccctggcct gggccatgtg gttcctgggg gatcgctggt ggctcgtgcc ggtgctgctc
      541 ggagcctggg cggtggccgg gttcgtggtc gccgcgatgg tcaccgggcg atggcgcccg
      601 gccttcctcc tcgcctcgct cgtcctcgcc ctcgcgtggc cggccgccgc cgctcccgcc
      661 gccgtcgtgg cccgggggct cgcggagctg gggggcaagg ggtttgggat cgtcagcggc
      721 cgagaggagg gcggcaccga ggccctgggc gagtggctgc acgcccgcat ccagcgcgcc
      781 gccgggctcc ccgacgacga gcccctgacc tttggcgacc tcgagagctc ggagatccac
      841 ctccgcatga tgaccaccga tgtcgccgcg gcccgacccg tgcggctccc cggcgagctg
      901 caggagttct cgttccgacc cgacgacgtg cgagggatcc tccccgagcg ggtcgcatcc
      961 gttctgatcg aacgggggcg cgggctcgcc gcccgacgcg gcctccccga gggggatctc
     1021 ctccccatgc cgcccaccga cgacatcccc atcctcctcg cgttccggat gtcgctggct
     1081 ttcccggggc tgttcacggc cgtgcgcctg tggtcggagc agatccaggg cccgccgcgc
     1141 gcgcactggt tctcggacgg ggggatctcg agcaacttcc cgatccactt cttcgactcg
     1201 tggatgccca cccggcccac gttcgggctg tcgctcggac ccgggaccgg gaaggccgtg
     1261 gagccgccgc cgaaggcgaa cgaggcgcgc ctgcccggct ggcgacgcgt ggagtcgctg
     1321 ggagggttcg tgaaggcgat cgtcgacacg atgcaggagt ggcgcgacac gatgcaggcc
     1381 gagctcccgg ggttccgcga ccgtgtgtgg gaggtgcggc tcgcggccga cgagggcggc
     1441 ttcaacatcg ggatgggccc cgagacgatc gctcggctca tcgagaaggg cgacgaggcg
     1501 ggccgctcga tccgcgagga gttcgactgg gaccagcacg tcttcacgcg gtacctgatg
     1561 gccatgcgcc gcctggagct ggggctcgtg ggggggatcg atccagccgg ccgtgagcag
     1621 agggggatcc gggccgcgtt cgacgcccgt cgcgagtggt tcgagcgacc gtcgcgggtc
     1681 gagctcttcg atcacgaccc cgagtggctg cggaaggcgg cggcgtcgac ggagcggctg
     1741 ctctccgaga tggaggggtg gagggagttc ggggggttcg cgggccagtc gccgcggccc
     1801 ccgggggaga tgcgcgtggt ccccgacgtc tagagggtca ggcggagagg gtcccgagcg
     1861 gccgggtcac ggcgtcggct gcccgggcca cctgctcggg cgacaggctc tggcgggacc
     1921 gctcgatgtg cgggacgagc gcgttgacgt ccagcacctt cgtctgcggc agctccagct
     1981 ccccgttctc cacgagcgct cgcgtcgaga cgatgatcgc ctccacccag gtgtgggaca
     2041 tgcccgcggc gtcgaggtgg cgtcgcaccg ccaacgcccc gctcaccgcc tgcctcctgg
     2101 tctcggccgc gtcacgcccg ttgtggagga ggcggcccgc gcgggcgagc agccgtccgc
     2161 tccaagcctt cgtctcgatg gcgaagaccc ccgtgggccc gatcacgacg tggtccacgt
     2221 tcccccgacc gatgtcgagg tcgtggatga cccggtagcc ggcggggagc cgctcgagcg
     2281 ccttggccac cgcccgctcg ccgtggagac cccggaccca agacgtgggg tcgaggagcc
     2341 ggccgtcctt ggtctgcccg tcgaagtaga ggatcagaag gaggccgggc aggatcatcg
     2401 gccaggagaa gagccccatc atgatcagca tcaagccgac ggcgaaggct gcgatcagga
     2461 cgatcgcgat ggagttgcgg cgaacctcca ggtacctgcc gggacggtcg gacatgctgg
     2521 gcctatcggc acatatcggg ccgaaccttg aacgctacgc cggcggctgg atgaagtgct
     2581 tgccgagcgt gtcggtctgc tgctggtacg aggagccgat cgcctctccg tagagcacct
     2641 cgggctcctc ggccatgcgc tcgaacgcga gcttgcagac gcgctggcgg tgctcgatca
     2701 ggaagggcac gtcgtgcgcc cgcacctcga gcgcggcgcg cgagccctgg aagcggccct
     2761 cgcggtcgaa cccgaagccg ggatcgaaga accccgcgta gtgcgtcctc agctccccgc
     2821 tggtcgggtc gtaggcggcc atctcggccg cgaagcccgg cgggatccgc accgcctcct
     2881 cggacagcag gaggtagaag tgctccggcg ccaggacgac ccggtcgccc tcctccttgt
     2941 agacgggctc ccagtaccgc tcccactcgt aagcccgggt gcgagccatc tcgatcgccg
     3001 gcgcgtagtc cttcgcccga tacccgacgc ggccgttcgc gtcgccccgg aggtcgagcc
     3061 ccaggaagag cccgtccgcg gtggcgaggt cctggtcggg cgccggggcg ccctccacga
     3121 acaggagcgg ctcgctcgcg tggacctcgc ggatctcctc gtcggtgagc ttcgcgttgc
     3181 cgatcgacag gcgcagctgg ctcagcgcga gcttgtgctg gaccttcacc gtgaaggaca
     3241 ggggcacgac ctcgaggtac agcggccccg cgtagccctc gcggatctcg tcgaagcgga
     3301 acccctcgtc ggtgatgacg cgggtgaaga cgtcgagccg gccggtggag ctcttggggt
     3361 tggccttgcc ccgcacgccc ggggggaggt tgagccgctc gatcaggggg atgaggtagg
     3421 gccggttcgt ctcgaggacg gcgccgtccc gccgcaggtc gagctcgtcg accacgaact
     3481 ccttgagctt gacctccacc ggccgcttgt cggggaggaa gctcgagcgg atccggtacg
     3541 cggtctcgcc gagccgaagg tcgacgctgg cgggctggat cgcctcgtcg gggacgcggt
     3601 agtcgccggc gtcgatgatg ccctggtcga tggcccgctc cagggcctgg ctggggagga
     3661 cgccgcgggc gccctcgggg aggccgagcc gagccggagt ggtgtcctgc gtctgggtca
     3721 tgacggtcgg gccatcatag ggtcggcgcg caggcggtcg ccacgctcaa gcgcgcttcg
     3781 tgacctcggc aacgtcggcc cccaggtgct cgatcaggtc gcccgcgacc acgtgaaggt
     3841 tcacgccgtg cgcgccgccc ccgatcgcca cgcgccggtc cgccgggatc gaggcatccg
     3901 cgatcacggg ccacggccgg gccgacccga gcggcgtgat cgcgccgcgc tcgtagccgg
     3961 tggcttcccg ggcctggtcc acggaaggca gcgacaggcg cctcacgccg aggtgcttcc
     4021 gcaggcgcgg ccagtcgatc tcccgacccc cgggcacgag cacgaacacg tagtcgtcct
     4081 cggctcgccg gaccacgatc gtccggagca gctgatccac ctcgatgccc tggagtgacg
     4141 cgctctcttc gacgctgctc ggccgctcgg tccgcacgac ctcgaagtcc agcgacgagc
     4201 gctcgaggct ccgcagggcg ggggtgacct gatccacgtg gggaggatag cggggcgggc
     4261 gggccccgcc gctggcggg
//
LOCUS       DASUTZ010000174        13425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1349101, whole genome shotgun sequence.
ACCESSION   DASUTZ010000174 DASUTZ010000000
VERSION     DASUTZ010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13425
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1349101"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VF097_07765"
     CDS             <1..501
                     /locus_tag="VF097_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEX6262728.1"
                     /translation="GARNMHHFPLLQEVGQTGKPIVLKRGLSATVEEWLMAAEYVAQQ
                     GNSDIVLCERGIRTFEPSTRNTLDLAGMIVAREASHLPVIADPSHATGQRSLVAPMAK
                     AAIAAGADGLMVEVHPRPDEALCDGPQALTDGDLDALVAEVAALARSLGRFVAEPAAS
                     VGESGA"
     gene            498..1502
                     /gene="trpS"
                     /locus_tag="VF097_07770"
     CDS             498..1502
                     /gene="trpS"
                     /locus_tag="VF097_07770"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020554601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX6262729.1"
                     /translation="MTDGKRVLSGIQPTGEIHIGNLIGAVRQWVADQDEFDTFYVIVD
                     LHAVTVPQEPGALREATVRMAASLIAAGLDPERCTLFVQSHVHEHAELAWVLGTLAGF
                     GELRRMTQFKDRVARYGEGSVSAGLFTYPVLQAGDILMYQADRVPVGADQRQHIELTR
                     DIAGRFNARYGETFVLPEAAIPRVGARIMDLQDGSSKMSASAPSQQGVLRLADEPDVL
                     RKKIRSAVTDSGREIVAREDKPEVSNLLTIMAVATGKDVSVLEVEYEGKGYGDFKGDL
                     AEAVIEFLRPIQERYAALRSDPAELARQLEIGAGKAQAVAAKTLKSVYERVGFLPRGG
                     "
     gene            complement(1499..2881)
                     /locus_tag="VF097_07775"
     CDS             complement(1499..2881)
                     /locus_tag="VF097_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6262730.1"
                     /translation="MPSEWAARCHNLEKVYRTATATVHALKGVTLELPRSRMTAVAGP
                     SGSGKSSLLRLLAGLDRPTSGTLEVDGVQLHRATGRARRAFRQRRVGYVFQRPSDNFI
                     PHLTVGQHLAMAARSAGEGAHPMEEVAEQLGIGGRLDHLPSALSGGEQQRAAVAQALV
                     RGADIIVADEPTADLDTTSTEILLARLSTLASEGLTIILATHDASVRRAADHVVELDH
                     GSVVSATRRHAPTSARASVRGAAAPLLVTATGLVRSFARGPETVHAVDHADLALREGE
                     VVALVGRSGSGKTTLLDLLGGWERPDAGTITWHGQEAAREPAWEDVSIVPQQLGLIEE
                     FSLRRNVEYPLRLAGVPGADRRVDGLFEKLRLAHLGDRFPGEVSVGEQQRAALARAVA
                     RRPRLLLADEPTGHQDRESARAVAVVLSEAASEGTCCLVATHEEHLSAFADRVIRMAD
                     GRLSADGGRP"
     gene            complement(2871..5579)
                     /locus_tag="VF097_07780"
     CDS             complement(2871..5579)
                     /locus_tag="VF097_07780"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX6262731.1"
                     /translation="MSRRVGLRGNPLWTTAPSALLHTPGLWTAVAAGTLLLAVAATFA
                     PAFLSATERELLTREIDNASVTRFGAGISFQISSLPLDRAVRGRPLHVEQRRVFSRRV
                     SEDPRLGSVVEGILSPEIEAAAQNGSRSRTAKLMFRTDGARHVRILEGEAEGVLIPEL
                     LARGLRAGPGASIDVRYQGGPPVRMTVGGVYETLYTTPRTGFWRSWSEEIWPCIGCDP
                     PPQPILVDRPQLLGAMSTLGVESAVFAWDAPLRVGGLTAPDAEGVARTFSAFLAEATG
                     VPGAPSEGAEILLCCGFRFEPFRTRTSLTSAMPAVLTIVDRRMIALEGPVRVLRLAGV
                     LVAVVTVAAAGAFATAARRREYTLLYATGLRTSSVALKGFLESVAPAAVGAAAGLGLS
                     LLLVAWLAPEGPMSGPALRSTIGWAAIVWAAAASLVGAVTAALFRRHAEVAHRTGMGL
                     PFDVALILVGVYLLDRITGTGALVVDPETGIETPSLALLALPVAGLAGIGGLAARAFH
                     VGLSRARERLRMRSEALYLAVHRLAGARRLALLLVATGAASIGLLLHALTVARSLDAT
                     VHAKSRVFVGSEVAAQIDPEFVPSREYGLPHTVVTRWRSGGRFASTGRQFDVLAVDPD
                     TVPGAAFWDDSFASVALEELMARLEAPGDGALPVIVAGHRDDLPERTTLILGRTEVSV
                     RIVATARAFPGMSSGDPLVVVGEEALRGGGPSGPLVGGRATGAVWVRASPRAAARRLI
                     AAGIHPDDILTARRVEDLPSIEAAVGTFAILAGLGLVSGLLVLVVLIMYVQARQRAQV
                     VSYALARRMGLRRGENVLALGLEVGTMLLTSFLVGAVVGVGISFFVVPQIDPLPAIAT
                     DPLMRSPVTASILTLTALAACTAIASWLAERAARRTNVAEVMRVAE"
     gene            complement(5644..6426)
                     /locus_tag="VF097_07785"
     CDS             complement(5644..6426)
                     /locus_tag="VF097_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262732.1"
                     /translation="MPKAELVFGNVLDGRVYEANGVEGPSFVYGFSAPGRALPFAVVR
                     HWKAPQGVVVEGFEIISPNGTTAYHSPTTTRRMPGQMDLTEIVDVVEDAVLPELGVYV
                     VSFLIDGEVEGQVEFQYVLQEAPDKLPKELEDGFRKTDVIWVGVDRDGRDETIPAWFA
                     YQQGRVYLLHAIKDDSPEQRVPGADDATDLLVITRHKYRDTRLSRLHATARVIEPSDG
                     DFARYAGILADRRRDRHGPPEEAIASWRQGHVILELIPLVPG"
     gene            complement(6464..7645)
                     /locus_tag="VF097_07790"
     CDS             complement(6464..7645)
                     /locus_tag="VF097_07790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX6262733.1"
                     /translation="MADEPFSDDIFKDVPLFRELQRVLFSGTGPVNWELARQVGIATA
                     VGDRPDPEPTDEDRAMLEPVVRAAELAVADLTGLEAPPEVSKIRPVRRATWVEASVTG
                     LRGLFEPGAEKLGKLMLEAPGHELGGESAPGLEGLAGLGALMGRMGPLLMGTQVGMVL
                     GMLGRRALGQYDLAVPREGGDALLWVIPALAELEREWDLPRDDVRAWVAVHEVAHSFQ
                     LGRDWVREHFLEQIRELAAGMEFDLSGLEERLAGVDLSDPERLQEAIGDPADLLGQKL
                     TDEQRLLMRRVQALMAAAEAHADHVLEVLGERMLTQAARIEEAMLRRREDRSEHERMA
                     ERLLGFELTDEHLRLGRAFVERVVEATDERVLASMWESAESLPSMPELEEPTLWLSRM
                     A"
     gene            7673..8527
                     /locus_tag="VF097_07795"
     CDS             7673..8527
                     /locus_tag="VF097_07795"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6262734.1"
                     /translation="MRIRPAEESDLRALAEIQVAGREWLARHYEPETLHQFPVDPDGA
                     TFTIRHVHKTGAVFVAEETDPVGFSSAIIREGVWFLSQLWVAPDHHGAGIGSALLDEA
                     LTWGHGSSIFSVVASPFPGAQTVYMRRSMFPIWHSMEFEGEAPRVEMPEGADELTAGD
                     QPWVNELDREVRGIARPEDHELFRAHARGIALRRGGDPLGYLYAWPEGKLGPAAIRDG
                     RDTPDLLRAGIAAGPSRLSLIVPTTNWGALQEAIRLRLRLVSRTVFMASRAMPDGSRY
                     LSSGGALA"
     gene            8590..9588
                     /locus_tag="VF097_07800"
     CDS             8590..9588
                     /locus_tag="VF097_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX6262735.1"
                     /translation="MRAGDLAIDLGTANTLVFSQERGIILNQPTVLAVNGRDGSVLAV
                     GEEAWEMIGRTPANIVAVRPMRSGAITDYDLTAQMMRLVLRRVGATRFHKPRVLLCVP
                     SAITEVERRAVSEATISSGARSVQLIEEPMAGAIGAGLPIHEPLGNLLVDVGGGTTEV
                     AMISMGGIVEARSTRVGGFDLDDAVQHYLRQRYGLAVGERAAETVKIAIGTAYPAGGV
                     PPYSIRGREITSGMPKEVQLTQEEIREAISDLVAHIVDAARDTLASSPPELGHDVLER
                     GMFLTGGGGLLRGLDMRIAQECEVPVHLTDSPLETVVLGAGKCLDLPADVPGLFAR"
     gene            9585..10436
                     /locus_tag="VF097_07805"
     CDS             9585..10436
                     /locus_tag="VF097_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011900493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEX6262736.1"
                     /translation="MTFSIVAADLEAGDWGVGVASKFPAVGAVVPWARAGVGAVATQA
                     WANTSFGPRGLELMAGGAEANEALRQMLDEDDGRVQRQVGMVDAHGIVATFTGEGCME
                     WAGGHTGEGFACQGNILVGPEVVEAMARAYEATEGDLLDRLLAAVTAGDAAGGDRRGR
                     QSAAILVVREGGGYGGYNDRYVDLRIDDHPDAVTELARLARVYDREILVRNDPLLEAT
                     PELVSDLQRRLSRLGRYGGEAHGAYDEATRAAIESFAGEYNLEGRLRDDELISGSLVS
                     EIRDVTP"
     gene            complement(10433..10723)
                     /locus_tag="VF097_07810"
     CDS             complement(10433..10723)
                     /locus_tag="VF097_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262737.1"
                     /translation="MELFLLALTAAVVGWLLWQLTEGSRDHRWTGMTEPPAPDADAGL
                     RSLPSTPDERTHPLVAATLLVFSILIVALGVAAGVYFAGRFVIERLGQMLGL"
     gene            10781..11746
                     /locus_tag="VF097_07815"
     CDS             10781..11746
                     /locus_tag="VF097_07815"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEX6262738.1"
                     /translation="MRRLLLSALPLVSLATALSLVDIGMFTIGPGSAEEVFPRIQLDG
                     VSVSEPRGRLLLTTVSIPRATVLHAAWAGWDPAVELVPEEAILGQRTEREHERLTRSQ
                     MDESKIAAVSIAATDLTDYPEEHGPGALVQNVLGGTAAAGKLFPGDLIVSVGDDPVDD
                     VDDVSRAIVATEARRALALTVEAGGEERTVRIRPRMSEEEGRPIIGVILIEPFPFEVE
                     IESGQIGGPSAGLMWTLGVYDLLSEEDLVDGRRIAGTGAIDLAGNVYAIGGAAQKVRA
                     ARDAGAEVFLVPRENLEEARAAGAGIRVVPVRDVRTALRFLRGTA"
     gene            11773..>13425
                     /locus_tag="VF097_07820"
     CDS             11773..>13425
                     /locus_tag="VF097_07820"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEX6262739.1"
                     /translation="MDRPPPPRRAAPRRRWLLWVGGAVLAIVVVFGALSGFYVDILWF
                     REVEQSGVFWTIFWSRALLAAVFGALFFALLYANLLIARAVRPRYQVFAPGEEAVDRY
                     RTLIEPYTRWALLGVSALLALFAAAGVAAQWEAYQQWRVAGRVSFGVIDPVFERDVAF
                     HVLSLPFQRFVQSWLFSSLVAVTVVTAAAHYLWGGIRFRAVAERVTPQVKAHLSVLLG
                     LIVLVRAWGYRLGQFDLLLSPRGTVTGASYTDVNAQLPALQLLVVMSIVVTVLFLVNI
                     RFRGWALPAIGLGLLVLTSVLAGAVWPAAIQRFRVDPQELQREREFITRNIEFTRQAY
                     GLDVVEAESFPAEPELTREELEENIGTVEDIRLWNPATLARAYFQLQRIRPYYEFVDV
                     DVDRYEIDGEKVLTMLAPREIAQDTIPGGQTWQNRHLVYTHGFGAAASRADQLDVAGT
                     PRFLLRDVPPAGDLAASLEQPRVYFGEETDVPYSLVRTEQQELDFPVGENEFARTTYD
                     GEGGIEVGGFFRRLAFAWRYRDVNLLISGLVRDDTKILINTDL"
BASE COUNT         1899 a         4768 c         4901 g         1857 t
ORIGIN      
        1 ggcgcccgga acatgcatca cttcccgctg ctccaggagg tgggacagac gggcaagccg
       61 atcgtcctga agcgcggcct gtcggcgacg gtcgaggagt ggctgatggc cgcggagtac
      121 gtcgcccagc aggggaacag cgacatcgtc ctgtgcgagc gggggatccg aaccttcgag
      181 ccgtccaccc ggaacacgct cgacctcgcc ggcatgatcg tggcccgcga ggcgagccat
      241 ctcccggtga tcgccgaccc gtcgcacgcc accggccagc gcagcctcgt ggcgccgatg
      301 gccaaggcgg cgatcgcggc gggcgccgac ggcctcatgg tggaggtcca cccccggccg
      361 gacgaagccc tctgcgacgg ccctcaggcg ctgaccgacg gggacctcga cgcgctggtg
      421 gcagaggtgg ccgcgctggc ccgatccctg gggcggttcg ttgcggagcc cgcggccagc
      481 gtgggagagt ccggggcatg accgacggga agcgtgtcct ctccggcatc cagcccaccg
      541 gtgagatcca catcggcaac ctgatcggcg ccgtccgcca gtgggtggcc gatcaggacg
      601 agttcgacac cttctacgtg atcgtggatc tccacgcggt cacggtcccg caggagcccg
      661 gcgccctccg ggaggccacg gtgaggatgg ccgccagcct gatcgcagcg ggcctggacc
      721 cggagcgctg cacgctcttc gtccagtcgc acgtacacga gcacgcggag ctggcgtggg
      781 tgctggggac actcgcgggg ttcggggagc tgcgccgcat gacgcagttc aaggaccggg
      841 tggcccgcta cggggagggc tcggtcagcg ccggactctt cacctacccg gtgctgcagg
      901 ccggggacat cctgatgtac caggccgacc gcgtccccgt gggagcggac cagcgtcagc
      961 acatcgagct gacgcgtgac atcgcggggc gcttcaacgc gcggtacggg gagacgttcg
     1021 tgctccccga ggccgccatc cctcgagtgg gggcccggat catggacctc caggacggct
     1081 cgtccaagat gtccgcctcc gcaccgtcgc agcagggagt cctgcggctg gcggacgagc
     1141 cggacgtgct ccgcaagaag atccgctcgg cggtgaccga ctcgggccgg gagatcgttg
     1201 cccgcgagga caagccggag gtctcgaacc tgctcacgat catggcggtg gccacgggca
     1261 aggacgtctc ggtcctagag gtcgagtatg aggggaaggg ctacggcgac ttcaagggag
     1321 acctggccga ggccgtgatc gagttcctgc ggccgatcca ggagcggtac gccgccctgc
     1381 ggagcgaccc ggccgagctg gcccggcagc tggagatcgg cgcggggaag gcccaggcgg
     1441 tcgccgccaa gacgctcaag tcggtctacg agcgggtcgg cttcctgcct cgcggcggct
     1501 agggacggcc tccgtccgcg gacagccgtc cgtcggccat cctgatcacg cggtccgcga
     1561 acgcgctgag gtgttcctcg tgggtggcca cgaggcagca ggttccctcg gaggcggcct
     1621 cgctcaggac cacggccacc gctcgggccg actcgcggtc ctggtgcccc gtcggctcgt
     1681 cggcgaggag gaggcgaggc cgcctggcca cggcccgggc cagggccgcc cgctgctgct
     1741 ctccgaccga gacctctcct gggaagcggt ccccgaggtg ggccaggcgc agcttctcga
     1801 acagcccatc caccctccga tcggcgcctg ggacgcccgc cagccgcagt gggtactcca
     1861 cgttcctgcg aagggagaac tcctcgatga ggccgagctg ctgggggacg atggagacgt
     1921 cctcccaggc cggctcacgg gccgcttcct gcccgtgcca cgtgatcgtc ccggcgtcgg
     1981 gccgctccca gccgcccagg aggtcgagga gcgtcgtctt gccggacccc gatcgtccca
     2041 ccagcgccac gacctctcct tcccgcagcg ccaggtcggc gtggtccacg gcgtgaacgg
     2101 tctcggggcc gcgcgcgaac gagcggacca gcccggtcgc ggtgacgagg agcggggccg
     2161 ccgccccccg gacgctcgca cgggccgatg tgggggcatg acggcgagtg gccgagacga
     2221 cggagccgtg atcgagctcg acgacgtggt cggccgcccg acgaacgctg gcgtcgtgcg
     2281 tggccaggat gatcgtcagg ccttccgagg ccagggtcga gagccgtgcg agcaggatct
     2341 cggtggacgt ggtgtcgagg tccgcggtgg gctcgtccgc gacgatgatg tcggcccctc
     2401 ggaccagggc ctgcgcgacg gccgcccgct gctgctcccc ccccgagagg gcggagggca
     2461 ggtggtccag gcgacccccg atgcccagct gctcggcgac ctcctccata gggtgggctc
     2521 cctctcccgc ggatcgggcc gccatcgcga ggtgctgtcc cacggtcagg tgggggatga
     2581 agttgtcgga ggggcgctgg aagacgtacc ccacccggcg ctggcggaac gctcgccggg
     2641 cgcgaccggt cgccctgtgg agctgcacgc cgtccacctc gagggtcccc gaggtcggac
     2701 gatccagccc ggccagcagc cggaggaggg acgacttccc gctgcccgac gggccggcaa
     2761 ccgccgtcat cctcgaccgc gggagctcga gcgtgacgcc cttcagagcg tgcaccgtag
     2821 ccgtggccgt ccggtacacc ttctcgaggt tgtggcatcg cgccgcccac tcactcggca
     2881 acgcgcatca cctccgccac attcgtccga cgggccgccc gctccgccag ccacgaggcg
     2941 atcgccgtgc aggccgccag ggccgtcagg gtcaggatcg aggcggtcac gggcgacctc
     3001 atgagggggt ccgtggcgat cgcggggagg gggtcgatct ggggcacgac gaagaacgag
     3061 atccctacac caaccacggc gcccactagg aaggaggtca gcagcatcgt ccccacctcg
     3121 agcccgaggg ccaggacgtt ctcgcctcgg cgaagcccca tccggcgcgc cagtgcatag
     3181 gagacgactt gagcccgctg tcgggcctgc acgtacatga tcaggaccac gagaacgagg
     3241 aggccgctca ccagccccag ccccgccagg atggcgaagg tgccgacggc cgcctcgatc
     3301 gagggcaggt cctcgacccg cctggccgtc aggatgtcgt ccggatggat ccccgccgcg
     3361 atgagccggc gcgccgcggc ccgaggggag gcccgcaccc agacggcccc ggtcgcgcgc
     3421 ccgccgacca gcgggcccga ggggccgccc cccctcagag cctcctcgcc cacgaccacg
     3481 agaggatcgc ccgagctcat gccggggaac gcccgagcgg tggcgacgat ccggacggag
     3541 acttcggtcc tgccgaggat cagcgtggtg cgctcgggga gatcgtcgcg gtggccggca
     3601 acgatcaccg gaagggctcc gtcgccgggg gcctccagtc gggccatcag ctcctcgagg
     3661 gccacggagg cgaacgagtc gtcccagaag gccgcccctg gcaccgtgtc ggggtccacg
     3721 gcgaggacgt cgaactgccg gcccgtggac gcgaaccgcc ctcccgaccg ccagcgggtc
     3781 accacggtgt ggggaaggcc gtactcgcgg ctggggacga actcggggtc gatctgcgcc
     3841 gccacctcgc tcccgacgaa gacccgcgac ttcgcgtgga cggtcgcatc cagcgaccgg
     3901 gccacggtca gggcgtggag cagcaggccg atgctggccg cgcccgtcgc taccaggagc
     3961 agggcgaggc gccgagctcc ggccaggcgg tggacggcga ggtagagggc ctccgatcgc
     4021 atccgcagcc tctctcgggc ccgtgagagg ccgacgtgga aggcgcgggc ggccagtcct
     4081 ccgatgccgg ccaggccggc gaccgggagc gcgagcagcg ccaggctggg ggtctcgatg
     4141 ccggtctccg gatcgacgac caacgccccc gtgccggtga tccgatcgag gaggtagacc
     4201 ccgaccagga taagggccac gtcgaatggc agccccatcc cggtccggtg ggcgacctcg
     4261 gcgtgccgtc ggaagagcgc tgccgtcacc gcgcccacca acgaggcggc cgccgcccag
     4321 acgatcgcgg cccagccgat cgtcgatcgg agggcgggcc cggacatcgg gccctccggc
     4381 gccagccagg caacgagcag gaggctcagt ccgaggccgg ccgcggcgcc gacggcggcg
     4441 ggtgccacgg actccaggaa gcccttcagc gccacgctgc tcgtccggag gccggtggcg
     4501 tacaggagcg tgtactcgcg ccgccgggcc gcggtggcga aggcccctgc cgcggccacg
     4561 gtgaccacgg cgaccagcac gccggccagc ctcagcacgc ggacggggcc ctccagcgcg
     4621 atcattcggc ggtccacgat ggtgaggacc gccggcatcg ccgaggtgag ggaggtcctc
     4681 gttcggaacg gctcgaagcg gaacccgcag cagagcagga tctccgctcc ctccgaggga
     4741 gcgcccggca ccccggtggc ctcggccagg aacgcggaga aggtgcgagc caccccctcc
     4801 gcgtcggggg ccgtgaggcc cccgacgcgg aggggggcgt cccacgcgaa gaccgccgac
     4861 tccacgccca gcgtcgacat cgcgcccagc agctggggcc ggtccacgag gatcggctgg
     4921 ggcggcgggt cgcagccgat gcagggccag atctcctcgg accatgaccg ccaaaagccc
     4981 gtccggggtg tcgtgtacag cgtctcgtac acccccccca cggtcattcg gacggggggc
     5041 ccgccctgat agcggacgtc gatcgaggcg ccggggccgg cccgcagccc ccgcgccagc
     5101 agctccggga tgaggacgcc ctccgcttcg ccctccagga tccggacgtg gcgggctccg
     5161 tccgtccgga acatcagctt ggcggtgcgg gacctcgatc cgttctgggc cgcggcctcg
     5221 atctcggggc tgaggatgcc ttcgaccacg gatcccaggc gcggatcctc cgacacccgc
     5281 ctcgagaaga cccgccgctg ctcgacgtgg aggggtcgac cgcgcacggc gcggtccagg
     5341 ggcaggctgc tgatctggaa cgagatcccc gcgccgaacc gcgtcacgga ggcgttgtcg
     5401 atctccctgg tcaggagctc ccgttcggtg gccgatagga acgcgggcgc gaacgtggcg
     5461 gcgaccgcca agaggagcgt gcccgccgcg acggccgtcc acaggcccgg ggtgtggagc
     5521 agcgccgagg gagcggtggt ccagagcggg tttcctcgca gacccaccct gcggctcatc
     5581 ggctcatccc gctcggcacc attggttcta cgtcctcccg gtcgagcggg atgtcctccc
     5641 ggctcagccg gggacgagcg ggatgagctc gaggatcacg tggccctgcc gccacgacgc
     5701 gatcgcctcc tccggcggcc cgtgacggtc tcgccgacgg tcggccagga tcccggcgta
     5761 ccgagcgaag tccccgtcgc tcggctcgat caccctcgcc gtggcgtgga gcctgctcag
     5821 cctcgtgtcg cggtacttgt gccgcgtgat caccaggagg tcggtggcgt cgtcggcccc
     5881 gggcacacgc tgctccggcg agtcgtcctt gatcgcgtgg aggaggtaga cgcgaccctg
     5941 ctgataggcg aaccaggcgg ggatcgtctc gtccctcccg tctcggtcca cccccaccca
     6001 gatcacgtcg gtcttgcgga agccgtcctc gagctccttg gggagcttgt cgggagcctc
     6061 ctggagcacg tactggaact cgacctgccc ctccacctcg ccgtcgatca ggaacgagac
     6121 gacgtacacc ccgagctccg gcaggaccgc gtcctccacc acgtcgacga tctcggtgag
     6181 gtccatctgg ccgggcatcc tgcgggtcgt ggtcgggctg tggtaggccg tggtcccgtt
     6241 cgggctgatg atctcgaagc cctcgaccac gacgccctgc ggcgccttcc agtggcggac
     6301 cacggcgaac ggcagggccc gccccggggc gctgaagccg tacacgaacg agggtccctc
     6361 gaccccgttc gcctcgtaga cccgtccgtc caggacgttc ccgaacacga gctccgcctt
     6421 gggcacggcg cgaggatagc agagggggtg gagcggctcg tgctcaggcc atgcgagaca
     6481 gccagagggt gggctcctcg agctccggca tcgagggaag gctctcggcg ctctcccaca
     6541 tcgaggccag cacccgctcg tcggtggcct cgaccactcg ctccacgaac gcgcggccca
     6601 ggcggaggtg ctcgtcggtc agctcgaacc cgaggaggcg ctcggccatc cgctcgtgct
     6661 cgctccggtc ctcgcggcga cggagcatgg cctcctcgat gcgtgcggcc tgcgtcagca
     6721 tccgctcgcc gaggacctcc aggacgtggt cggcgtgggc ctcggccgcg gccatcagcg
     6781 cctggacccg ccgcatcagg aggcgctgct cgtcggtgag cttctgcccc aggaggtcgg
     6841 ccgggtcccc gatcgcctcc tggagccgct cggggtcgga gaggtccacc ccggccagcc
     6901 gctcctcgag ccccgagaga tcgaactcca tcccggccgc cagctctcgg atctgctcca
     6961 ggaagtgctc ccggacccag tcgcgaccca gctggaagct gtgcgccacc tcgtggacgg
     7021 cgacccaggc ccggacgtcg tcgcggggga ggtcccactc gcgctccagc tccgccagcg
     7081 ccgggatcac ccagaggagg gcgtcccctc cctcgcgggg gaccgccagg tcgtactgcc
     7141 ccagggcgcg gcgccccagc atccccaata ccatcccgac ctgagtgccc atgaggagcg
     7201 gccccatccg gcccatgagg gcgcccaggc cggccaggcc ctccagaccc ggggcgctct
     7261 cgccgccgag ctcgtggccg ggcgcctcca gcatcagctt ccccagcttc tcggcgcctg
     7321 gctcgaagag gccccggagg ccggtcacgc tcgcctcgac ccacgtcgcg cgtcggaccg
     7381 gacggatctt ggagacctcg ggcggggcct cgagcccggt gaggtcggcc accgcgagct
     7441 cggcggcgcg gacgaccggc tcgagcatcg ctcggtcctc gtcggtgggc tcggggtcgg
     7501 ggcggtcgcc gactgcggtg gcgatcccca cctgtcgggc cagctcccag ttcacggggc
     7561 ccgtgcccga gaagaggact cgctggagct cccggaagag gggcacgtcc ttgaagatgt
     7621 cgtcggagaa gggctcgtcg gccatcgggt ccattgtagg ctcgccctcg ccatgcggat
     7681 ccgcccggcc gaggagtcgg acctccgcgc cctggccgag atccaggtgg ccgggcgcga
     7741 gtggctggcg cgccactacg agcccgagac cctgcatcag ttccccgtcg acccggacgg
     7801 ggccacgttc acgatccggc acgtgcacaa gacgggcgcg gtgttcgtgg ccgaggagac
     7861 cgatcccgta gggttctcct cggcgatcat ccgggagggg gtctggttcc tctcgcagtt
     7921 gtgggtcgcg ccggatcatc atggggcggg catcgggtcg gccctgctcg acgaggccct
     7981 gacctggggc cacggctcct cgatcttcag cgtcgtggcc agcccgttcc cgggcgcgca
     8041 gaccgtctac atgcgacgct cgatgttccc catctggcac tcgatggagt tcgagggaga
     8101 ggctcccagg gtcgagatgc ccgagggagc ggacgagctg acggcggggg accagccgtg
     8161 ggtgaacgag ctcgaccgcg aggtgcgcgg catcgccagg cccgaggacc acgagctgtt
     8221 tcgcgcgcac gcgaggggga tcgccctgcg gcgagggggt gatcccctcg ggtacctgta
     8281 cgcgtggccg gaagggaagc tcgggccggc cgcgatccgc gacggccggg acacccccga
     8341 ccttctccgc gccgggatcg cggccggccc gagccgcctc tcgctcatcg tccccacgac
     8401 gaactggggc gcgcttcagg aggcgatccg cctgcgcctc cgcctcgtct cgaggacggt
     8461 gttcatggcc agccgcgcca tgcccgacgg ctcccgctac ctctccagcg gcggcgccct
     8521 agcctgagag gccgcccggg gccttgggtg ggccggggcc ttgggtgggc cgggggctag
     8581 aatggccgga tgcgagccgg ggatctcgcc atcgatctgg ggaccgccaa cacgctcgtc
     8641 ttctcccagg agcgcggcat catcctcaac cagccgaccg tgctggccgt gaacggccgg
     8701 gacgggtcgg tgctggccgt cggcgaggag gcctgggaga tgatcggccg cacccccgcc
     8761 aacatcgtcg ccgtccgccc catgcgcagc ggggcgatca cggactacga cctcaccgcc
     8821 cagatgatgc ggctcgtcct gcgccgcgtc ggggccacgc ggttccacaa gccccgggtc
     8881 ctgctgtgtg tgccgtcggc gatcaccgag gtggagcgcc gcgcggtgag cgaggccacg
     8941 atctcgagcg gtgcccggtc ggtccagctg atcgaggagc cgatggccgg ggcaatcggg
     9001 gcgggcctcc ccatccacga gccgctgggg aacctcctcg tggacgtggg cggcggcacg
     9061 accgaggtgg ccatgatctc gatgggaggc atcgtcgagg cccgctccac gagggtggga
     9121 ggcttcgacc tcgacgacgc ggtccaacac tacctccggc agcggtacgg gctggcggtc
     9181 ggcgagcgtg ccgccgagac cgtgaagatc gcgatcggga cggcctaccc cgccggcggc
     9241 gtccccccct actcgatccg gggccgtgag atcacgtcgg ggatgcccaa ggaggtccag
     9301 ctcacccagg aggagatccg cgaggccatc tcggacctcg tcgcgcacat cgtcgacgcc
     9361 gcgcgggata cgctcgcctc gagcccgccc gagctgggcc acgacgtcct ggagcgcggg
     9421 atgttcctca cgggaggcgg ggggctgctg cgggggctgg acatgcggat cgcccaggag
     9481 tgcgaggtgc cggtgcacct caccgactcg ccgctggaga cggtcgtgct gggggctggg
     9541 aagtgtctcg acctgccggc cgacgtcccg gggctgttcg cgcgatgacg ttctcgatcg
     9601 tggccgcaga cctcgaggcg ggggactggg gcgtgggcgt cgcctcgaag ttccccgcgg
     9661 tgggggccgt ggtgccgtgg gcccgcgccg gggtcggggc ggtggccacg caggcctggg
     9721 ccaacacctc gttcggcccc cgcggcctcg agctgatggc cggcggggcc gaggcgaacg
     9781 aggcgctcag gcagatgctg gacgaggacg acgggcgcgt gcagcggcag gtcgggatgg
     9841 tggacgcgca cggcatcgtg gcgacgttca ccggcgaggg gtgcatggag tgggccggcg
     9901 ggcacaccgg ggagggcttc gcctgccagg ggaacatcct cgtgggcccg gaggtggtgg
     9961 aggcgatggc ccgggcctac gaggcgaccg agggggatct cctcgaccgg ctgctggcgg
    10021 cggtcaccgc cggcgatgcc gccggcggcg accgccgcgg acgccagtcg gccgcgatcc
    10081 tcgtcgtccg ggagggaggc gggtacggcg gctacaacga ccgatacgtg gacctccgga
    10141 tcgacgacca ccccgacgcg gtgaccgagc tcgccaggct cgcccgagtc tacgaccgcg
    10201 agatcctggt gcggaacgac ccgcttttgg aagccacccc cgagctcgta tccgacctcc
    10261 agcggcgcct atcccggctc ggacgctacg ggggcgaggc ccacggcgcg tacgacgagg
    10321 ccacgcgggc ggcgatcgag agcttcgcgg gcgagtacaa cctcgagggg aggcttcgcg
    10381 acgacgagct gatctcgggc tccctcgtga gcgagatccg ggacgtgacg ccctagagcc
    10441 cgagcatctg gccgagccgc tcgatcacga agcggccggc gaagtacacg cccgcggcga
    10501 cccccaaggc cacgatcagg atcgagaaga cgaggagcgt cgcggcgacg agcggatgcg
    10561 tccgctcgtc gggggtgctg gggagcgatc ggaggccggc gtcggcgtcc ggagcggggg
    10621 gctcggtcat cccggtccag cggtggtcgc gcgacccctc ggtcagctgc cagagcagcc
    10681 atccgacgac cgcggccgtc agcgccagga ggaagagctc catgaccccg atgttcccat
    10741 ccgaagggat tccatgctcc ccggcgtacg attgatccgc atgcgccgac tcctgctctc
    10801 cgcgctgccg ctcgtctccc tcgcgacggc cctctctctc gtggatatcg gcatgttcac
    10861 catcgggcct ggatcggccg aggaggtctt cccccggatc cagctcgacg gtgtgagcgt
    10921 cagcgagccc agaggcaggc tcctgctgac gaccgtcagc atccctcgtg ccaccgtcct
    10981 ccacgccgcg tgggcgggat gggacccggc cgtcgagctg gtccccgagg aggcgatcct
    11041 gggccagcgc accgagcgcg agcacgagcg gctcacgcga tcccagatgg acgagagcaa
    11101 gatcgcggcc gtcagcatcg cggccacgga cctcaccgac tacccggagg agcacggccc
    11161 gggcgcgctc gtgcagaacg tcctcggggg gaccgccgcc gcggggaagc tgttcccggg
    11221 ggacctcatc gtgtcggtcg gggacgatcc cgtcgacgac gtggacgacg tgtcgcgggc
    11281 gatcgtcgcc accgaggccc ggcgggcgct ggccctcacc gtcgaggccg gaggcgagga
    11341 gcgcaccgtc cgcatccgcc cgcggatgtc cgaagaagag ggccgtccca tcatcggggt
    11401 gatcctgatc gagcccttcc ccttcgaggt cgagatcgag agcggccaga tcggcgggcc
    11461 ctcggccggg ctgatgtgga cccttggcgt ctacgacctc ctgtccgagg aggacctggt
    11521 ggacggacgg cggatcgcgg gcaccggcgc gatcgacctg gccgggaacg tctacgcgat
    11581 cggcggcgcc gcccagaagg tacgcgcggc gagagacgcg ggcgccgagg tcttcctcgt
    11641 tccgagggag aacctggagg aggcgcgggc tgcgggagcc gggatccggg tcgtccccgt
    11701 gagggacgtc cggaccgccc tgcgcttcct ccgcggaacc gcctgatgcc cgtctgatag
    11761 gctggccacg ttttggaccg acctccacct ccccggcgcg cggcgccccg gcgacgctgg
    11821 ctgctgtggg tcggcggcgc cgtcctggcg atcgtggtcg tcttcggcgc gctctccggc
    11881 ttctacgtgg acatcctctg gttccgggag gtcgagcagt cgggcgtctt ctggacgatc
    11941 ttctggtcga gggccctcct ggcggccgtg ttcggcgccc tcttcttcgc gctcctctac
    12001 gccaacctgc tgatcgcgcg ggccgtgcgg ccgcgctacc aggtgttcgc gccgggggag
    12061 gaggccgtgg accgctaccg cacgctcatc gagccctaca cgcggtgggc gctcctgggg
    12121 gtctcggccc tcctggcgct gttcgccgcg gccggggtgg ccgcgcagtg ggaggcctat
    12181 cagcagtggc gcgtggcggg gagggtctcg ttcggcgtga tcgatcccgt cttcgagcgc
    12241 gacgtggcct tccacgtgct ctcgctcccg ttccagcggt tcgtgcagtc gtggctgttc
    12301 tcgtcgctgg tggccgtcac cgtggtgacc gcggccgcgc actacctgtg gggagggatc
    12361 cggttccggg ccgtggccga gcgcgtgacc ccgcaggtga aggcgcacct ctcggtgctt
    12421 cttgggctca tcgtgctggt ccgggcctgg ggctaccgcc tggggcagtt cgacctcctc
    12481 ctgtcgccgc ggggcaccgt caccggcgcc tcgtacacgg acgtgaacgc ccaactcccg
    12541 gcgcttcagc tcctcgtggt gatgtcgatc gtggtcaccg tcctcttcct cgtgaacatc
    12601 cggttccgcg ggtgggccct gccggcgatc gggctggggc tgctggtgct gacctcggtg
    12661 ctggccggcg ccgtctggcc cgccgcgatc cagcgcttcc gcgtcgatcc ccaggagctc
    12721 cagcgggagc gggagttcat caccaggaac atcgagttca cgcggcaggc ctacgggctc
    12781 gatgtggtcg aggcggagtc gttcccggcg gagccggagc tgacccgtga ggagctcgag
    12841 gagaacatcg ggaccgtgga ggacatcagg ctgtggaacc cggccaccct ggctcgcgcc
    12901 tacttccagc tccagcggat ccgcccctac tacgaattcg tggacgtcga cgtggatcgt
    12961 tatgagatcg acggcgagaa ggtgctgacg atgctcgcgc cccgtgagat cgcgcaggac
    13021 acgatcccgg gcgggcagac gtggcagaac cggcacctcg tgtacacgca cggcttcggc
    13081 gcggcggcca gcagggcgga ccagctggac gtggccggca ccccgcgctt cctcctgcgg
    13141 gacgtccccc cggcgggcga tctcgcggcc tcgctggagc agccccgggt ctacttcggg
    13201 gaggagaccg acgtccccta ctcgctcgtg cgcaccgagc agcaggagct cgacttcccg
    13261 gtgggcgaga acgagttcgc gcgtaccacc tacgacggtg aggggggcat cgaggtgggc
    13321 gggttcttcc gtcgcctcgc cttcgcgtgg cggtaccgcg acgtgaacct gctgatctcc
    13381 gggctcgtgc gggacgacac gaagatcctg atcaacaccg atctc
//
LOCUS       DASUTZ010000175         5610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1480456, whole genome shotgun sequence.
ACCESSION   DASUTZ010000175 DASUTZ010000000
VERSION     DASUTZ010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5610
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480456"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4895
                     /locus_tag="VF097_07825"
     CDS             <1..4895
                     /locus_tag="VF097_07825"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX6262740.1"
                     /translation="EIDAFRFEALLDEARGLATSDPAGAIRRYREALALWRGPALEDL
                     SDQPSLRGTITRLEELRMAATEELISAEMDLGRHVELVPELETLVALHPLRERLWGQL
                     MLALYRSGRQGDALGAFLRAREILAEELGIDPSPELQRLQEGILRQDLPLEPAGEPLR
                     GYRLLEQVGAGAFGTVHRAFQPQVGREVAVKVIHPELANDAEFIRRFESEAQLVARLE
                     HPHVVPLYDYWREPDGAYLVMRFLRGGSLRDALSEGALDPARAATILEQTGQALAAAH
                     RQGIVHRDVKPANILLDDEGNAYLSDFGIAKDLVAPHRPAEGGVPSPLAYYISPEELH
                     GEVPTPKSDIYNLGLVLYELLVGRHPYADAPPERLDEMQLREPVPSLVAMRPDLPASL
                     DGVIQRATAKEPGDRYPDVAAFAAASRTALGTAREGVTPAVGVGDRNPYKGLHPFLEA
                     DAPDFFGREELAERLLSRMAEVEGEPRFLGVVGPSGSGKSSLVRAGLIPALRRGALPG
                     SERWFVVDMIPGARPFEELAAALTRIAVDPLPGLLEVLERDDSGLARAGAEILPEGSE
                     LLLVIDQFEELFTLVEDEDARARFLAAIHTAVTSPESRVRAVVTLRADFYDRPLLYKP
                     FGDVLAANSEAVTPLSPEEVERAVSGPAEAVGVSSDPALLADIIADVAARPGALPLLQ
                     HALLELFDRRRDGLLTVEAYREIGGISGALGSRAEDLYGRLSEAGKEAARQLFLRLVT
                     LGEDGSSEDTRRRVLRAELASLEVDPGAMEAAVDGFGARRLLFFDRDPTTRGPTVEVA
                     HEALLREWGRLRGWIEAAREDVKTHRRLAAAAAEWTEGGRETSFLLRGERLAGFEAWS
                     ETSRLALTRDEREYLQAALAKREAERVEEEARKAREAALEHRSIYRLRALVAVFAVLA
                     LVASGLTALTLNQRGRAEREARIATARELSAAAVANLDVDPERSILLALEAVDRTRSV
                     DGSVLPEAEEALHRAVAASRIILSVPDVGGEVAWSPDGSTFVTEGPQESGIIDVRDAE
                     TGVSLRRWHGHDADVKNVTFNHDGSMLATTGGDGAARVWDPETGEELAVMLQEPSDSD
                     VAGAAFSPDGTLLAAAWRTEGVVRIMDVKTGEIRPEIEAATYWHVSFSPDGTRLAVTI
                     NDAPRTVVVVDVDSGEERFRLEGHVLPSLDVAWSPDGRWIATASWDQTARIWDAETGE
                     PRFTLHGHTGGVVNLDWSHDGTRLVTGGTDGTAKVWEVTPTGARQVLSLTADALSTGV
                     WGVAFSPDGSRVMTGAGLSAPTVQVWDVSLGGGAQWGTFPLASARSGSGLSGVAFTPE
                     GDLVAVDEDAVRTWDLQTGEEALWIKHGGHSQFFRIEVSPDGALIAGVTDQGVTVWDA
                     DTGDTVLTLPSRHVVLTWPSDVAWSPDGTHLAVSSDRDGILILDRAGDEVAFLPEPGF
                     EVPALEFSPDGRLLAAATFYRDRVDLEASGVKIWDWRDGEVVQEILTAAVELTFDPGG
                     ERLATAETWGSRAHVWDVATGDSLVTLTGHTGFLWDVEFAPDGTTIATASWDGTVRLW
                     DVDSGRQELTLRGHESVVFQARFSPDGSRLASASTDGTVRVWALDLDDLIRIANRKLT
                     RGLTDEECRHYLRQHPCPTS"
     gene            complement(5020..5096)
                     /locus_tag="VF097_07830"
     tRNA            complement(5020..5096)
                     /locus_tag="VF097_07830"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5060..5062),aa:Arg,seq:acg)
     gene            complement(5099..5189)
                     /locus_tag="VF097_07835"
     tRNA            complement(5099..5189)
                     /locus_tag="VF097_07835"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5151..5153),aa:Ser,seq:gct)
     gene            5308..>5610
                     /locus_tag="VF097_07840"
     CDS             5308..>5610
                     /locus_tag="VF097_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262741.1"
                     /translation="MVPAAGHGPSTPALFRLPAIGALAGTAAGITAAAGSRVAMRISA
                     AVAHPALSEAGVRTENGNVVGVITVGGTLGLILFGTILTAGAGLLYLALRRWLPGPP"
BASE COUNT          843 a         1947 c         2039 g          781 t
ORIGIN      
        1 ccgagatcga cgcattccgg ttcgaggccc tgctcgacga agcgagaggg ctcgcgacga
       61 gcgacccggc cggcgcgatc cgccgctacc gagaggccct ggccctgtgg agagggcccg
      121 cactcgagga cctgagcgac cagccctcgc tccgcggcac catcactcgc ctcgaggagc
      181 tgcggatggc ggccaccgag gagctgatct cggccgagat ggacctgggg cgtcacgtgg
      241 agctcgtgcc ggagctggag accctcgtgg ctctccaccc actgcgggag cgcctgtggg
      301 gacagctgat gctggccctg taccggtccg gccggcaggg ggacgccctg ggggcgttcc
      361 tgcgggcccg ggagatcctc gctgaggagc tggggatcga cccctcgccg gagctccagc
      421 gcctccagga ggggatcctc agacaggacc tccccctgga gccggccggg gagccgctcc
      481 gcggataccg gctgctggag caggtgggag cgggcgcgtt cgggacggtg caccgagcgt
      541 tccagcctca ggtcggacgc gaggtcgccg tcaaggtcat ccatccggag ctggccaacg
      601 acgcggagtt catccgccgg ttcgagtccg aagcacagct ggtggcccgg ctggagcacc
      661 cgcacgtggt gccgctatac gactactggc gggagccgga tggcgcctac ctcgtgatgc
      721 ggttcctgcg cggagggagc ctccgcgatg cgttgagcga gggagcgctc gacccggcgc
      781 gagccgcgac gatcctcgaa cagaccggcc aggccctggc cgcggcgcat cggcagggca
      841 tcgtccaccg ggacgtgaag cccgccaaca tcctcctgga cgacgagggg aacgcctacc
      901 tctcggactt cggcatcgcc aaggacctag tggcgcccca tcggcccgcc gaggggggcg
      961 tcccgagccc cctcgcgtac tacatctccc cggaggagct ccacggagag gtcccgacgc
     1021 ccaagagcga catctacaac ctcggcctcg tcctctacga gctgctggtc ggacggcacc
     1081 cgtacgcgga tgcgcctccg gagcggctgg acgagatgca gctccgggag ccggtgccat
     1141 ccctcgtcgc gatgcggccc gaccttccgg cgtcgctgga cggcgtcatc cagcgagcca
     1201 cggcgaagga gccgggcgat cggtatccgg acgtggccgc gttcgcggcg gcctccagaa
     1261 ccgcgctcgg gacagcccgg gagggggtca cgccggccgt gggggtcggt gaccggaacc
     1321 cctacaaggg gctccacccc ttcctcgagg ccgatgcccc ggacttcttc ggacgcgagg
     1381 agctcgccga gcggctcctc tcccggatgg cggaggtcga gggggaaccc cggttcctcg
     1441 gggtcgtcgg cccctcgggg agcgggaagt cctcccttgt gcgggcgggc ctgatccccg
     1501 ccctccgacg cggggcactc ccgggatcgg agcgctggtt cgtggtcgac atgatccccg
     1561 gagcccgccc gttcgaggag ctggccgcgg cgctcaccag gatcgctgtt gatccgctcc
     1621 cgggcctcct cgaggtgctc gagcgagacg actcggggct cgcacgggcg ggcgcggaga
     1681 tccttcccga ggggtccgag ctcctcctgg tcatcgacca gttcgaggag ctgttcaccc
     1741 tggtcgagga cgaggacgcg cgggcccggt tcctggcggc catccacacc gccgtcacga
     1801 gcccggagag ccgagtgcgg gccgtcgtga ccctccgggc cgatttctac gatcgcccgc
     1861 tcctctacaa accgttcggc gacgtcctgg ccgcgaacag cgaggcggtc accccactct
     1921 cccctgagga ggtcgagcgg gccgtctccg gacccgctga ggcggtgggg gttagcagcg
     1981 accccgcgct cctcgccgac atcatcgccg atgtggcggc gagaccgggg gccctcccgc
     2041 tcctccagca cgccctcctc gagctgttcg accgccgcag ggacggactc ctcaccgtgg
     2101 aggcctaccg cgagatcgga gggatctccg gagcgttggg gagcagggcc gaggatcttt
     2161 acgggcggct cagcgaggcc gggaaggagg ccgcccggca gctcttcctc cgtctcgtga
     2221 ccctcggcga ggacgggagc agcgaggaca cccggcggcg cgtgcttcgg gcggagctcg
     2281 cctcgctgga ggtcgacccc ggggccatgg aggccgccgt cgacggcttc ggggcccgca
     2341 ggctcctgtt cttcgaccgg gacccgacca ccaggggacc caccgtggag gtggcccacg
     2401 aggccctgct ccgcgagtgg gggcggctcc gagggtggat cgaggccgcc cgggaggacg
     2461 tcaagaccca ccgccgcctg gccgccgccg ccgccgagtg gacggaggga ggaagggaga
     2521 ccagcttcct cctccggggg gagcggctcg ccgggttcga agcctggagt gagacgtccc
     2581 gcctcgcgct gaccagggac gagcgggagt acctccaggc cgccctggcc aagcgggaag
     2641 ccgaacgggt cgaggaagag gcgaggaagg ctcgagaggc ggcgctcgag catcggtcca
     2701 tctaccggtt gcgagccctg gtagcagtgt tcgccgtcct ggccctggtg gcctcgggcc
     2761 tgacggctct caccctcaac cagcgggggc gcgccgagag agaggcccgc atcgccaccg
     2821 cccgagagct gtccgcggcc gcggtcgcca accttgacgt cgaccccgag cgaagcatcc
     2881 tgctggccct ggaggcggtg gaccggaccc ggtccgtgga cggctccgtg ttgccggagg
     2941 cggaggaggc gctccatcgc gccgtcgccg cgagccgcat catcctcagc gtccccgacg
     3001 tcggcggtga ggtggcctgg agccccgacg ggagcacctt cgtcaccgag ggaccgcagg
     3061 agagcggcat catcgacgtc cgcgacgccg agacgggtgt ctccctgcgc agatggcatg
     3121 ggcacgacgc cgacgtgaag aacgtcacct tcaaccacga cgggtccatg ctggccacga
     3181 ccgggggcga cggcgccgcc agggtctggg atccggagac cggcgaggag ctcgcggtca
     3241 tgctgcagga gccctcagac tcggacgtgg cgggcgcggc gttcagcccc gatgggaccc
     3301 tgctggccgc cgcgtggcgg accgagggcg tggtgcggat catggatgtg aagaccggcg
     3361 agatccgccc ggagatcgag gcagccacgt actggcacgt gtcgttcagc ccggacggga
     3421 cgaggctcgc tgtgacgatc aacgatgccc cccggacggt cgtcgtcgtg gacgtggact
     3481 ccggcgagga gcggttcagg ctcgagggcc acgtcttgcc ctccctcgat gtcgcgtgga
     3541 gtccggacgg gaggtggatc gccaccgcga gctgggacca gaccgctcgg atctgggacg
     3601 cggagaccgg agagcctcgg ttcacgctcc acggccacac cggcggcgtg gtgaatctcg
     3661 actggtccca cgacgggaca cgcctggtca ccggcggcac agacgggacg gcgaaggtct
     3721 gggaggtcac gccgactggg gcgcggcagg tgctctcgct caccgcggac gccctaagca
     3781 ccggcgtctg gggcgtggcg ttctcacccg acggctcccg ggtcatgacg ggagccgggc
     3841 tctcggctcc aaccgtccag gtctgggacg tgagccttgg cggcggcgcc cagtggggga
     3901 cctttccgct ggcctccgcc cggagtggaa gcggtctgag cggcgtggcg ttcacccctg
     3961 aaggtgacct cgtggccgtg gatgaggatg cagtcaggac ctgggacctc cagaccggag
     4021 aggaggcgct ctggatcaag catggtggtc actcccagtt cttccggatc gaggtcagcc
     4081 ccgatggggc gctgatcgca ggggtgaccg atcagggggt cacggtctgg gacgctgaca
     4141 ccggggacac ggtgctcacg ctccccagcc gacacgtggt cttaacatgg ccgtcggatg
     4201 tcgcgtggag ccccgacggc acccacctcg ccgtctcgtc ggaccgagac gggatcctca
     4261 tcctcgaccg tgcgggggac gaggtcgcgt tcctcccaga gcccggcttc gaagtaccgg
     4321 ccctcgagtt cagccccgat ggacgcctcc tggccgccgc gaccttctac cgcgaccggg
     4381 tcgacctcga ggccagcggc gtcaagatct gggactggcg ggatggtgag gtcgtgcagg
     4441 agatcctcac ggccgccgtc gaactcacct tcgacccggg cggcgagcgc ctcgccaccg
     4501 ccgaaacctg ggggtcgcgg gcgcacgtct gggacgtggc taccggggat tccctggtga
     4561 cccttacggg gcacaccggg ttcctctggg atgtcgagtt cgccccggac gggaccacca
     4621 tcgccaccgc gagctgggac gggaccgtac gcctgtggga cgtggattcc ggccggcagg
     4681 agctcacgct caggggtcac gaatccgtgg tctttcaggc gcggttcagt ccggacggat
     4741 ccaggctcgc ctcggccagc accgacggga cggtccgcgt ctgggcgctc gacctcgacg
     4801 acctcatccg gatcgcgaac cggaagctga cgcgggggct caccgacgag gagtgccgcc
     4861 attacctgcg gcagcacccc tgccccacga gctgagctcg atgaaccagg aacccgctcc
     4921 ccgtggctca gagcacacga agcgtgccgg acagaaccca gcccgtgaga tcgacgcagc
     4981 gcccgaaagt ggtcgctgaa ctgcgaaaac gcctcatcgt ggcgcgcccg ggaggactcg
     5041 aacccccagc cttctgatcc gtagtcagac gctctatcca attgagctac gggcgcagcg
     5101 gaggcggcgc gattcgaacg cgcgaggggg attgaacccc caacgggatt agcaatcccg
     5161 cgccatagac cagactaggc gacgcctccg ccgttcagtc tagccgacgc caggcgcgcc
     5221 gccagggggg ctgatccaga cggggaccgt gacgatcaga gcctggctct tgacctggac
     5281 acacaggaga gcgagcatga cccggtcatg gtcccagcgg cggggcatgg cccgtctacg
     5341 ccggcgctct tccgcctgcc ggcgatcggg gcgctggccg gcacggccgc cggcatcacg
     5401 gcggctgcgg ggtcccgcgt cgcgatgagg atcagcgctg ccgtcgcgca tccggcgctc
     5461 tcggaggctg gggtgcggac ggagaacggc aacgtggtcg gggtcatcac ggtcggcggc
     5521 acgctggggc tgatactctt cggcacgatc ctcaccgccg gagcggggct gctctacctg
     5581 gctcttcgcc gctggctccc cgggcctccg
//
LOCUS       DASUTZ010000176         4898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_833032, whole genome shotgun sequence.
ACCESSION   DASUTZ010000176 DASUTZ010000000
VERSION     DASUTZ010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833032"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2125
                     /locus_tag="VF097_07845"
     CDS             <1..2125
                     /locus_tag="VF097_07845"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX6262742.1"
                     /translation="GSAVTVVLKRVENPLEFGIVIADDEGRIERFLEKPGWGEVFSDT
                     INTGIYVVEPEVLNHVPAGEEYDFAGELFPLLLDKGLPMHGFVAEGYWTDVGNLEAYL
                     QVHRDILDQKVRVDIGGYRTETGVWRGEGSEVDPDAQIHGPVLIGENARIEAGASVHE
                     YSVLGKGVVVKTGAFVHRAIVHDGAYIGSSASIRGCVVGKNVDVKHGARLEEEVVVAD
                     EVYVGEGAVLSPRVKVFPFKRIDPGALVSKSIVWETTTQQGLFGERGVAGLVNIDITP
                     EMAMRLALAFASTMPKKSVLTAGRDPTRTARVIKRAMVAGVNAAGVNAHDLELVPTPV
                     TRFYARTSRAEGGFVVRTAPGDPQSVEIVFIDERGIDIGPSAQRKVERGFFRDDLRRA
                     FHHEIGELTFPARGREYYLDGILGVVDAETVRGARPKVVIDYGWGSAAITAPLVLGRL
                     GADTLAINATVEEERSVLTRQQQEQHLLALGGLVRAGGADLGVLMDPTGERMWLADER
                     GRVIGLSQALLAFVRLVADAVPAPTIAVPVASSRVVEEIVGAGGGKVVWTGISPAALS
                     TASDAGDVAFAGAEGGGYIFPSFLPAYDAVMSLSKLLELLIRSGRSLGEIVDGLPSTH
                     VVRIDVPCPWEAKGAVMRRLVERFAEEDVVTIDGVKRFRGRDWALIVPHPLEPVVRVW
                     AEADEEEAARALAADHASLVEELRG"
     gene            2146..2874
                     /locus_tag="VF097_07850"
     CDS             2146..2874
                     /locus_tag="VF097_07850"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEX6262743.1"
                     /translation="MREARVHIGLWIVLALFGFLVSVGFLHERSREETSPARRAELER
                     LIEQRRGAIHELSTQVSRLSSRVDDVRKRAGAGSSEVDDLVRRLEGLRREVGLEDVEG
                     PGVVVELSDSAKPATTRDELTDLRIQDVDLQLVANALWRSGAEAVSVNGRRLTATSAI
                     RKAGSAILVNYRAVSSPYRIAAIGDQEALLDGLEGSEIAERFAVWKEIYGLGFSMRAE
                     DELRAPALPGLQEFRYAVPVEGER"
     gene            2871..3200
                     /locus_tag="VF097_07855"
     CDS             2871..3200
                     /locus_tag="VF097_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013476763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HEX6262744.1"
                     /translation="MIALIALLAGVAIGLLLQPTLPAALAPFLPVIVVAALDTVLAGL
                     LSRLEGGYREGEFIVAFLSNTLLAAFIVWVGDRLGTPDLTIGVVVVFGIRMFQSLAGI
                     RHALFRD"
     gene            3200..3937
                     /locus_tag="VF097_07860"
     CDS             3200..3937
                     /locus_tag="VF097_07860"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEX6262745.1"
                     /translation="MDYLDLHDPGPRRPTMRPPSRTQTRIALVVVALAVGLLVGVQAR
                     TRGDATARLAAEAPEDLARILSDLNEEADQLARQLADLRLRLFRYEGTAERGDLALRD
                     ARKTLRDLQVLAGTTEVRGPGLRVDIADQMARVSWEPMLDLVQELRDAGAEAIAVSGV
                     RVVASSWFGPGDAGITIDGEHLVPPYRIEAIGDPPGLREALGIPGGPISLIEAQPGVS
                     ISAQELDRMRLPALRREITFRYARPAS"
     gene            3950..4348
                     /gene="gcvH"
                     /locus_tag="VF097_07865"
     CDS             3950..4348
                     /gene="gcvH"
                     /locus_tag="VF097_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008341044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX6262746.1"
                     /translation="MDYPEDLRYTKEHEWAAAEDGNRVRVGITDFAQDALGDVVYADL
                     PEEGTEVRAREPFGEVESTKSVSDIFSPVSGRVAERNAALEDQPELVNEDPYGEGWMV
                     VIEMSDPAELEELLTAEGYRSFAEGGEEGA"
     gene            4437..4892
                     /locus_tag="VF097_07870"
     CDS             4437..4892
                     /locus_tag="VF097_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006540372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX6262747.1"
                     /translation="MYCTRCGERNPDDARFCAHCGHALQDDTTVGLAATEIEDEAGEE
                     IFLPLDELQAGQALLVVKRGPTAGSRILVDKDVTTAGRHPESDIFLDDITVSRRHAEF
                     RRKEGRFFIHDMGSLNGTYVNRDRVDSTQLASGDEVQIGKFKLVFFMGE"
BASE COUNT          716 a         1589 c         1889 g          704 t
ORIGIN      
        1 gggctcggcc gtgaccgtgg tgctgaagcg cgtggagaac cccctggagt tcgggatcgt
       61 gatcgccgac gacgagggcc ggatcgagcg cttcctggag aagccgggct ggggcgaggt
      121 cttcagcgac acgatcaaca cggggatcta cgtcgtcgag cccgaggtcc tgaaccacgt
      181 gccggcgggc gaggagtacg acttcgcggg cgagctcttc ccgctgctgc tggacaaggg
      241 cttgcccatg cacgggttcg tggccgaggg ctactggacc gacgtgggca acctcgaggc
      301 atacctccag gtgcaccgcg acatcctcga ccagaaggtg cgcgtggaca tcggcggcta
      361 ccgcacggag accggggtgt ggcgggggga gggatccgag gtcgatccgg acgcgcagat
      421 ccacgggccc gtcctcatcg gcgagaacgc ccggatcgag gccggcgcct cggtccacga
      481 gtactcggtt ctgggcaagg gcgtcgtggt gaagaccgga gcgttcgtcc accgggccat
      541 cgtccacgac ggcgcctaca tcggatcctc cgcgtcgatc cggggctgcg tcgtgggcaa
      601 gaacgtggac gtgaagcacg gcgcccgcct cgaggaggag gtcgtggtag ccgacgaggt
      661 gtacgtgggc gagggggccg tcctcagccc gagggtgaag gtcttcccct tcaagcggat
      721 cgacccgggg gcgctcgtct ccaagagcat cgtgtgggag acgaccaccc agcagggact
      781 gttcggcgag cggggcgtgg ccgggctcgt caacatcgac atcacccccg agatggcgat
      841 gcgcctggcg ctggccttcg ccagcacgat gccgaagaag tcggtcctga cggcagggag
      901 ggaccccacc cggaccgccc gggtgatcaa gcgggccatg gtcgcgggcg tgaacgcggc
      961 cggcgtcaac gcgcacgacc tggagctggt cccgaccccg gtgacgcgct tctacgccag
     1021 gaccagccgc gccgaggggg ggttcgtcgt ccgcaccgcg ccgggcgacc cgcagtcggt
     1081 cgagatcgtc ttcatcgacg agcgagggat cgacatcggc ccgtcggccc agcggaaggt
     1141 ggagcgaggc ttcttccgag acgatctccg ccgggcgttc caccacgaga tcggggagct
     1201 caccttcccg gcgcggggtc gcgagtacta cctggacggg atcctgggcg tggtcgacgc
     1261 ggagacggta cgaggagccc ggcccaaggt cgtgatcgac tacgggtggg gctccgcggc
     1321 gatcaccgcc cctctggtgc tggggaggct gggcgcggac acgctggcca tcaacgccac
     1381 ggtcgaggag gagcgctcgg tcctgacccg tcagcagcag gagcagcacc tgctggcgct
     1441 cggagggctg gtccgcgcgg gcggtgccga cctcggcgtg ctgatggatc ccaccggcga
     1501 gcggatgtgg ctcgcggacg agcgggggcg cgtcatcggg ctgagccagg ccctgctggc
     1561 gttcgtgcgc ctcgtggccg acgcggtccc tgcgcccacg atcgcggtgc cggtcgcctc
     1621 cagccgggtc gtggaggaga tcgtgggcgc ggggggaggc aaggtcgtct ggacggggat
     1681 ctcgccggcg gcgctctcga cggcgagcga cgcgggcgac gtcgccttcg ccggcgccga
     1741 gggcggcggc tacatcttcc cgagcttcct ccccgcctac gatgccgtca tgagcctgtc
     1801 gaagctgctc gagctcctga tccgcagcgg ccgttccctg ggggagatcg tggacggcct
     1861 ccccagcacc cacgtcgtgc ggatcgacgt cccctgtccg tgggaggcga agggggccgt
     1921 gatgcggcgg ctcgtcgagc gcttcgccga ggaggacgtc gtgacgatcg acggcgtgaa
     1981 gcggttccgg ggccgggact gggcgctgat cgtgccgcac cccctcgagc ccgtcgtgcg
     2041 ggtatgggcg gaggccgacg aggaggaggc ggcccgagcg ctcgccgccg accacgcctc
     2101 gctggtggag gagctgcgag ggtagcgtcg gctggagggc gtgcgatgag ggaggcgcgg
     2161 gtccacatcg gtctctggat cgtcctggcc ctcttcgggt tcctcgtgag cgtgggtttc
     2221 ctccatgagc ggagccgcga ggagacgtcg ccggcccgcc gggccgagct ggagcgcctc
     2281 atcgagcaac gccgtggggc gatccacgag ctctccacgc aggtgagcag gctctcgtcg
     2341 cgggtggacg acgtccggaa gcgagcgggg gcggggtcct cggaggtcga cgacctcgtc
     2401 cggaggctcg agggcctgcg ccgggaggtg gggctggagg acgtggaggg ccccggcgtc
     2461 gtggtggagc tctccgacag cgccaagccg gcgacgaccc gcgacgagct gaccgacctc
     2521 aggatccagg acgtggacct ccagcttgtg gcgaacgcgc tgtggcgatc gggggccgag
     2581 gccgtctcgg tgaacggccg gcgcctcacc gcgacctcgg cgatccggaa ggcggggagc
     2641 gcgatcctcg tgaactaccg ggcggtgtcg tcgccctacc ggatcgcagc gatcggggac
     2701 caggaggcgc tgctcgacgg gctggaggga tcggagatcg ccgagcggtt cgcggtgtgg
     2761 aaggagatct acgggctcgg cttctcgatg cgggccgagg acgagctccg tgcccccgcg
     2821 ctcccgggtc tccaggagtt ccggtacgcg gtgccggtgg agggggaacg atgatcgcac
     2881 tgatcgctct gctggcgggg gtggcgatcg ggctgctcct ccagcccacc ctgccggccg
     2941 cgctggctcc cttcctcccc gtcatcgtcg tggccgctct cgacaccgtg ctggccggcc
     3001 tcctgtcccg cctcgagggc gggtaccggg agggggagtt catcgtggcg ttcctctcga
     3061 acaccctgct ggcggcgttc atcgtctggg tgggcgatag actgggaacg cccgacctga
     3121 cgatcggcgt ggtggtcgtg ttcgggatcc ggatgttcca gagcctggcg gggatccgcc
     3181 acgccctctt tcgagactga tggactacct cgacctccac gatccgggcc cgaggcggcc
     3241 gacgatgcgg ccccccagcc gcacgcagac gaggatcgca ctggtggtcg tcgcgctggc
     3301 tgtgggcctg ctggtcgggg tgcaggcgag gacccgaggg gacgccacgg cccggctggc
     3361 ggcggaggcg cccgaggact tggcccggat cctctccgac ctcaacgagg aggccgacca
     3421 gctcgcgcgc cagctggccg atctgcggct gaggctgttc cgctacgagg ggacggccga
     3481 gcgaggggac ctcgcgctgc gcgacgcgcg gaagacgctc cgggacctcc aggtcctggc
     3541 cgggaccacc gaggtgcggg ggccgggcct ccgggtcgac atcgcggatc agatggcccg
     3601 ggtgtcgtgg gagccgatgc tcgatctcgt ccaggagctg cgcgacgcgg gggccgaggc
     3661 catcgccgtg agcggcgtgc gcgtcgtcgc atcgagctgg ttcggccccg gggacgccgg
     3721 gatcacgatc gatggggagc acctcgtgcc gccgtaccgg atcgaggcga tcggcgaccc
     3781 gccggggctg cgcgaggccc tggggatccc cgggggcccg atctcactga tcgaggccca
     3841 gccgggggtg tcgatctcgg cgcaggagct cgaccgcatg cgcctgccgg cgttgcgtcg
     3901 agagatcacg ttccgatacg ctcgcccggc ctcgtaacgg aggaggacca tggactaccc
     3961 agaggacctg cgctacacga aggagcacga gtgggccgcg gccgaggacg ggaacagggt
     4021 ccgggtcggc atcacggact tcgcgcagga cgccctgggc gacgtcgtct acgcggacct
     4081 gcccgaggag gggaccgagg tgagggcccg cgagcccttc ggcgaggtcg agtcgacgaa
     4141 gtcggtctcg gacatcttca gcccggtgtc gggccgcgtg gccgagcgca acgcggccct
     4201 cgaggaccag cccgagctgg tgaacgagga tccctacggc gaggggtgga tggtcgtgat
     4261 cgagatgtcc gatccggccg agctcgagga gctgctcacc gccgaggggt accggtcgtt
     4321 cgccgaggga ggagaagagg gggcctgagc ccctccagcc ctcgttgacc gttctgggac
     4381 cgtccgatac agtcgcctca gcctctccct gaggcttcgg gtcggatcgg aggccgatgt
     4441 actgcacccg ctgcggcgag aggaaccccg acgacgcgcg cttctgcgcg cactgcggcc
     4501 acgccctcca ggacgatacg acggtcggcc tggccgcgac cgagatcgag gatgaagcgg
     4561 gggaggagat cttcctcccg ctggacgagc tccaggccgg gcaggccctc ctcgtggtga
     4621 agcggggacc caccgcgggg tcgcgcatcc tcgtggacaa ggacgtgacg acggccgggc
     4681 gccaccccga gagcgacatc ttcctcgacg acatcacggt gtcgcggcgg catgcggagt
     4741 tccgccgcaa ggaggggagg ttcttcatcc acgacatggg gagcctcaac ggcacgtacg
     4801 tcaaccgcga ccgggtggac agcacgcagc tcgccagcgg ggacgaggtg cagatcggca
     4861 agttcaagct cgtgttcttc atgggggagt gatggcgg
//
LOCUS       DASUTZ010000177         3597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_833043, whole genome shotgun sequence.
ACCESSION   DASUTZ010000177 DASUTZ010000000
VERSION     DASUTZ010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833043"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="VF097_07875"
     CDS             complement(<1..789)
                     /locus_tag="VF097_07875"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX6262748.1"
                     /translation="MPGRIRVGTSSWTDKTLLESGWYPPEADTPEERLKHYASVFPVV
                     EVDSTYYGLPSERNSGLWVERTPPDFTFHVKAFSAMTGHPTRQRGLPKAIREALPEDA
                     KDRLYPKDLPQDALDEIWEIFRRALMPLHSAGKLGVVMFQYPEWFVPSKRSRGEILRA
                     RERLPDYDIAVELRQAKWFESEEDAKKTLEFLRGEEIPFVCVDMPQGFSSSIPPIAEA
                     TSEKYGLVRMHGRREETWGKRGVPVIERFKYDYAKEELKGWASRV"
     gene            complement(795..1079)
                     /locus_tag="VF097_07880"
     CDS             complement(795..1079)
                     /locus_tag="VF097_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262749.1"
                     /translation="MKIRGTCGNCGKEFLVEQVVASHGHCPWCGKAFTRDYTANLARA
                     LAQADAAGDVLEDALEAIADIHPAMDLDEESVLEAIREPLRAQRRRRTPA"
     gene            complement(1076..1471)
                     /locus_tag="VF097_07885"
     CDS             complement(1076..1471)
                     /locus_tag="VF097_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262750.1"
                     /translation="MKALVISADPDVRRQLEVALRSAGRQASDQWEFLEASNGVQGIR
                     VAWRELPDLVVADEIASGAGAFAAAKDLRGQMRPFPGAIIIVLARPQDAWLARWAGAD
                     AWVTKPVDPFELADHVVRLTGRQTVEEGA"
     gene            complement(1499..1897)
                     /locus_tag="VF097_07890"
     CDS             complement(1499..1897)
                     /locus_tag="VF097_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEX6262751.1"
                     /translation="MRRRLQGARVARLATADPTGRPHLVPIVFALVEDLIVTVVDEKP
                     KRTKRLQRLENVRANPLVTVLVDHYEEDWSRLWWVRVRGDARVHEEGPERERALEALA
                     GKYAQYRDAAPHGPAILIEAQEWLGWSASG"
     gene            complement(1924..2094)
                     /locus_tag="VF097_07895"
     CDS             complement(1924..2094)
                     /locus_tag="VF097_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262752.1"
                     /translation="MADERKTQTKDEGEEQNGRVCGECGMKLSSYNPGPNCWQHTIGH
                     PWRGPSAKPRYD"
     gene            complement(2170..3228)
                     /locus_tag="VF097_07900"
     CDS             complement(2170..3228)
                     /locus_tag="VF097_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017569223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEX6262753.1"
                     /translation="MTDGNGGGNGDGVRGRQLTLTKKDFTSDQEVRWCPGCGDYAILS
                     TVQGFLPELGVPPEDIVFVAGIGCSGRFAYYMDTYGVHGIHGRAPALATGIATTRPDL
                     SVWVITGDGDGLSIGGNHLIHALRRNVDITILLFNNQIYGLTKGQYSPTSEQGKVTKS
                     TPFGSVDYPFNPVALALGAEATFVARTIDNDRKHSTEVLRAAAQHKGSAFIEIFQNCN
                     VFNDGAFAAVRDKQAGAQQNQIRLEHGRPIRFGKDGERGVVQRSDGSLEIVAVQDAGE
                     EGLLVHDMHRAEPALAFSLAHLSERPTGPTPIGIFREISRPVYGEAMDAQLEQAKEKM
                     GEGQLTTLLHSGDTWTVE"
     gene            complement(3225..>3597)
                     /locus_tag="VF097_07905"
     CDS             complement(3225..>3597)
                     /locus_tag="VF097_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HEX6262754.1"
                     /translation="LQKVKNVADSLPPLEVDDPDGDAELLVVGWGSTQGTIKAALAAV
                     RDRGKKVAHVHLVHLHPFPNDLEEILRRYPKLLVPEMNMGQLSRLLRAEYLVDAQLYS
                     KVQGQPIFVEELTEEIVRRLA"
BASE COUNT          508 a         1293 c         1211 g          585 t
ORIGIN      
        1 gacccgcgag gcccacccct tcagctcctc cttcgcgtag tcgtacttga agcgctcgat
       61 cacggggacg ccccgcttgc cccacgtctc ctcgcgccgc ccgtgcatcc gcacgagccc
      121 gtacttctcg ctcgtggcct cagcgatcgg agggatcgag gaggagaacc cctgcggcat
      181 gtccacgcac acgaacggga tctcctcgcc ccgcaggaac tcgagcgtct tcttcgcgtc
      241 ctcctcggac tcgaaccact tcgcctggcg cagctccacg gcgatgtcgt agtcgggcag
      301 ccgctcgcgc gcccgcagga tctccccgcg cgagcgcttg gagggcacga accactccgg
      361 gtattggaac atcaccacgc ccagcttccc cgcggagtgg agcggcatca gggcgcggcg
      421 aaagatctcc cagatctcgt cgagcgcgtc ctgggggagg tccttcgggt agaggcggtc
      481 cttcgcgtcc tcgggcaggg cctcgcggat cgccttgggg aggcctcgct ggcgggtggg
      541 gtggccggtc atcgccgaga acgccttgac gtggaacgtg aagtcgggcg gggtccgctc
      601 cacccagagc cccgagttcc gctccgaggg aagcccgtaa taggtcgagt ccacttcgac
      661 caccgggaag acggacgcgt agtgcttcag ccgctcctcg ggggtatcgg cctcgggcgg
      721 gtaccacccg gactcgagca gggtcttgtc ggtccaggac gaggtaccga cccggatcct
      781 cccgggcatg gcggtcaggc cggggtgcga cggcggcgct gggcccggag cggctcccgg
      841 atggcctcga gcacgctctc ctcgtcgaga tccatcgccg gatggatgtc ggcgatcgcc
      901 tccaacgcgt cctcgaggac gtcgccggcg gcatcggcct gggccagggc ccgggccagg
      961 ttggccgtat agtcccgggt gaacgccttc ccacaccagg ggcagtgccc gtgcgacgcc
     1021 accacctgct cgaccaggaa ctccttcccg cagttgccgc acgtgcctcg gatcttcacg
     1081 ctccctcctc cacggtctga cgacccgtga gccgcaccac gtggtcggcc agctcgaagg
     1141 ggtccaccgg cttcgtcacc caggcgtcgg ccccggccca cctcgccagc cacgcgtcct
     1201 ggggacgggc cagcacgatg atgatcgcgc ccgggaaggg tcgcatctgg cctcggaggt
     1261 ccttcgccgc ggcgaaggca cccgcgcccg aggcgatctc gtccgccacg accaggtcgg
     1321 gcagctcgcg ccatgccacg cggatcccct ggacgccgtt cgaggcctcg aggaactccc
     1381 actgatcgga cgcctgccgg ccggcgcttc ggagcgccac ctcgagctgg cgccgcacgt
     1441 cgggatccgc ggagatgacc agggctttca cggggctcat cctagcgcac gggcccggtc
     1501 accccgacgc tgaccatcca agccactcct gggcctcgat caggatcgcg gggccgtgcg
     1561 gagccgcgtc gcgatactgc gcgtacttcc cggccagcgc ctcgagtgcc cgctcgcgct
     1621 ccggaccctc ctcgtggacg cgggcgtccc cccgcacccg aacccaccac aggcgcgacc
     1681 agtcctcttc gtagtggtcc acgagcacgg tcacgagcgg gttggcccgg acgttctcca
     1741 gccgctggag ccgcttcgtc cgcttcggct tctcgtcgac cacagtgacg atcaggtctt
     1801 cgaccagggc gaagacgatc ggcacgaggt gggggcggcc ggtcgggtcc gccgtggcca
     1861 gccgcgctac gcgcgcgccc tggagccgcc ggcgcatctc cgcgagctcc aagggagccc
     1921 gcgtcagtcg tagcggggct tggccgaggg gccgcgccag gggtggccga tcgtgtgctg
     1981 ccagcagttg ggcccaggat tgtaggagga gagcttcatg ccgcactcgc cacacacgcg
     2041 gccgttctgc tcttcgccct cgtccttcgt ctgcgtcttg cgctcgtcgg ccatggccct
     2101 cagcataaca cccgaggcaa atcgggccct cctgacctgt ggtttcgtcc cggtagccac
     2161 cggttcgggc tattcgaccg tccaggtgtc gcccgagtgg agcagggtcg tgagctgacc
     2221 ctcgcccatc ttctccttcg cctgctcgag ctgggcgtcc atggcctcgc cgtagacggg
     2281 gcgcgagatc tcgcggaaga tcccgatcgg cgtcgggccg gtcggtcgct ccgagaggtg
     2341 cgccagcgag aaggccagcg cgggctcggc tcggtgcatg tcgtggacga gcagcccctc
     2401 ctcgccggcg tcctggacgg cgacgatctc gagggacccg tcggagcgct ggacgacgcc
     2461 gcgctcaccg tccttcccga agcggatcgg ccggccgtgc tccaggcgga tctggttctg
     2521 ctgggcgccg gcctgcttgt cgcgcaccgc ggcgaacgcc ccgtcgttga agacgttgca
     2581 gttctggaag atctcgatga aggccgagcc cttgtgctgg gcggccgcgc gcaagacctc
     2641 cgtggagtgc ttccggtcgt tgtcgatcgt ccgggcaacg aacgtggcct cggcccccag
     2701 cgccagcgcc acggggttga agggatagtc caccgagccg aacggcgtgg acttcgttac
     2761 cttcccctgc tccgaggtgg gcgagtactg ccctttcgtc aacccgtaga tctggttgtt
     2821 gaagagaagg atcgtgatgt cgacgttgcg gcgcagggcg tggatcagat ggttgccgcc
     2881 gatcgagagg ccgtcgccgt cgcccgtgat cacccagacc gagagatcgg ggcgcgtcgt
     2941 cgcgatcccg gtggcgagcg ccggcgccct cccgtggatc ccgtggaccc cgtacgtgtc
     3001 catgtagtag gcgaaccgcc ccgagcaccc gatgcccgcc acgaacacga tgtcctccgg
     3061 gggcacgccg agctccggca ggaacccctg cacggtcgag aggatcgcgt agtcgccgca
     3121 gcccgggcac cagcgcacct cctggtcgga ggtgaagtcc ttcttcgtca gggtgagctg
     3181 gcgcccgcgg acgccgtcgc cgttgccgcc gccgttgccg tcggtcatgc gagcctcctg
     3241 acgatctcct ccgtcagctc ctcgacgaag atgggctgac cctgcacctt cgagtacagc
     3301 tgggcgtcca ccaggtactc cgcgcggagc agccgcgaga gctggcccat gttcatctcc
     3361 gggacgagga gcttggggta cctgcggagg atctcctcca gatcgttcgg gaacggatgg
     3421 aggtgcacga ggtgcacgtg cgccaccttc ttccctcgat cgcggacggc cgcgagggcc
     3481 gccttgatcg tcccctgcgt ggatccccag ccgacgacga gcagctccgc gtcgccatcg
     3541 ggatcgtcga cctcgagcgg cggcagcgag tcggcgacgt tcttcacctt ctgcagc
//
LOCUS       DASUTZ010000178         4904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1481452, whole genome shotgun sequence.
ACCESSION   DASUTZ010000178 DASUTZ010000000
VERSION     DASUTZ010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481452"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..1241
                     /locus_tag="VF097_07910"
     CDS             57..1241
                     /locus_tag="VF097_07910"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX6262755.1"
                     /translation="MSGSVIVAGARTPIGKFGRALKDLKAVDLGALAIREALTRAGVA
                     GDQIDYVIMGQVIQAGSGQITARQAAIAAGIPKEVPAITVNKVCLSGLNAVALADQLI
                     RAGEIEVAVAGGMESMSNAPYLLPEARWGVRMGDATLVDSMIYDGLYSTFTGNEMGAD
                     TDEVNRELKIPRDVQDEWAARSHQRAAAAWDEGRMADEVVAVEVPQRKSDPITVDRDE
                     GIRPDTTVGGLARLRPAFHEDGTITAGNASQLSDGGAALVVMSRQRAAAMGLEPLAEI
                     VAHGMSADRYPYLHTVPATAIEHALKKAGPAASDLGLVEINEAFAAVAVNATSMLGVD
                     EEIVNVNGGAVALGHPIGMSGARLVLTLALEMGRRGVELGAASLCGGGGQGDALILRR
                     PA"
     gene            1241..2395
                     /locus_tag="VF097_07915"
     CDS             1241..2395
                     /locus_tag="VF097_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6262756.1"
                     /translation="MEPFSLPEELELFRKAAREVCDERIAPRAAEIDETEEFPHDVHR
                     VLVESELMAVGYPEEHGGAGGGSLTTAILVEEVARASASCSLIPLVSRLGAIPVMLEG
                     TREQKDELLGGISRGEHQMSYGLTEPAAGSDAVAMQTRYEPAAGSSFRLNGAKRFISN
                     AGVSDRITVFATRDPAERSKGISAFIVHDDDAGFSVGKSEKKMGIRGSPTRELYFDNV
                     EIPEDRLIGEEGKGFSYAMRTLDYSRPTIAAQALGIAQGAFEVAARYSREREQFGKPI
                     GELQGIQFLLADMAIQIEAARLLTYKAAAACDAQDPQLTYLASIAKAHASDTAMQVTT
                     DAVQVLGGYGYMREYGVERMMRDAKITQLYEGTNQIQRVVIARQIAKAFG"
     gene            2395..2901
                     /locus_tag="VF097_07920"
     CDS             2395..2901
                     /locus_tag="VF097_07920"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX6262757.1"
                     /translation="MRAKTPGESHVELVDQMQVTDANLMGNVHGGVIMKMVDNAAGLA
                     AIKHSEGPVVTAAMDEMSFIEPVYVGDVVTVKATVNEAFTTSMEVGVRVEAENLHTRE
                     VKHTSSAYLVFVAMDADGEPRPVPPLQPETEEERRRQQEAKLRREARLARKEAIEQAR
                     AGGERAPS"
     gene            complement(2894..4834)
                     /locus_tag="VF097_07925"
     CDS             complement(2894..4834)
                     /locus_tag="VF097_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX6262758.1"
                     /translation="MSDIVWEPTEEIIERANVTRFMRAHGIETYEELVRRSQDDVEWF
                     WDAVVEDLDVPFFEPYERVLDTSEGIPWAKWFVGGKLNLAHVTCDRWAEETPDAVAVL
                     WEGEDGAVRSVTYRELREMADRLANGLASLGVGQRDAVGIFMPMAPETVAAVLACAKL
                     GAVYLPIFSGYAADAVATRLRDADAKVLLTADGFPRRGTEVAMKEIADEAADVSPTVE
                     KVVVWSRLGRSDLPWRADRDLRWEELTEKAAHSFETLSVDSEHPLFIAYTSGTTGRPK
                     GSVHVHGGFTVKIAEEVAYQTDVQRGDVLFWVSDLGWIMGPWEITGAGALGATVFLYE
                     GAPNHPEPDRIWDMVERHRITHLGISPTLIRALIPAGDEWVERHDLSSLRILGSTGEP
                     WNPDPYLWFLEKVGGGRCPIINLSGGTEIGACFLSPLPINSLKPATLRGPALGMAIEV
                     WGPDGKSVPPGEVGELVCTKPWPAMTRGVWGDPQRYLDTYWSRWPDVWVHGDWASVDE
                     DGFWFLHGRSDDTMNIAGKRLGPAEVESALAEHPLVAESAAVGVPHEVKGESIWCFVV
                     TRPGTELEGELASELQGVVVEHLGKAYKPDRIVFVEELPRTRSAKILRRAIRAAVAGQ
                     DPGDLSSLENPGALEKIRASLS"
BASE COUNT          769 a         1683 c         1769 g          683 t
ORIGIN      
        1 ggcgcgcgcg tagaatggac atcccacagc gtctggccgc gaaaggagca cgaccgatgt
       61 ccggttccgt catcgtcgcg ggcgcgcgca cgccgatcgg gaagttcggg cgcgcgctca
      121 aggacctgaa ggccgtcgac ctcggggcgc tcgcgatccg cgaggcgctg acccgggccg
      181 gcgtggccgg cgatcagatc gactacgtga tcatgggcca ggtgatccag gcggggtccg
      241 gacagatcac ggcgcgtcag gcggcgatcg cggcggggat ccccaaggag gtccccgcga
      301 tcacggtcaa caaggtctgc ctctccgggc tgaacgctgt ggcgctcgcc gatcagctga
      361 tccgggccgg cgagatcgag gtcgcggtgg cgggggggat ggaatcgatg tcgaacgcgc
      421 cgtacctcct ccccgaggcc cgatggggcg tgcggatggg cgacgccacc ctcgtcgact
      481 cgatgatcta cgacggcctc tactccacgt tcaccggcaa cgagatgggc gccgacaccg
      541 acgaggtcaa ccgtgagctg aagatccctc gcgacgtcca ggacgagtgg gcggcgcgca
      601 gccaccagcg ggccgcggcc gcgtgggacg aggggcgcat ggccgatgag gtcgtggccg
      661 tcgaggtccc gcagcggaag agcgacccga tcaccgtgga ccgggatgag ggcatccgtc
      721 ccgacacgac cgtggggggc ttggccaggc tccgtcccgc tttccacgag gacggcacga
      781 tcaccgcggg caacgcttcc cagctctccg acggcggcgc ggctctcgtg gtgatgtcgc
      841 gccagcgggc tgcggcgatg ggcctcgagc cgctggccga gatcgtggcc cacggcatga
      901 gcgccgaccg ctacccgtac ctccacacgg tgccggccac cgcgatcgag cacgcgctga
      961 agaaggcggg ccccgccgcg agcgacctcg ggctcgtgga gatcaacgag gccttcgccg
     1021 cggtggcggt gaacgccacg agcatgctcg gcgtggacga ggagatcgtg aacgtcaacg
     1081 gcggcgccgt cgccctgggt cacccgatcg ggatgagcgg cgcccggctc gtcctgacgc
     1141 tggccctcga gatggggcgg cgcggcgtgg agctcggggc ggcctcgctc tgcggtggcg
     1201 gcggccaggg cgacgcgctg atcctccgcc gcccggcctg atggagccct tctcgctccc
     1261 ggaggagctg gagctcttcc gcaaggcggc ccgcgaggtc tgcgacgagc ggatcgcgcc
     1321 gcgcgcggcc gagatcgacg agaccgagga gttcccccac gacgtgcacc gcgtgctcgt
     1381 ggagagtgag ctgatggcgg tgggctaccc ggaggagcac ggcggggccg gcggcggctc
     1441 gctgacgacg gcgatcctcg tggaggaggt cgcgcgggcc tccgcgagct gctcgctgat
     1501 cccgctcgtc tcccggctcg gggcgatccc cgtgatgctc gaggggacga gggagcagaa
     1561 ggacgagctg ctcggcggca tcagtcgcgg ggagcaccag atgtcctacg gactcaccga
     1621 gcccgcggcc ggctccgacg ccgtcgcgat gcagacccgc tacgagccgg ccgcgggctc
     1681 gagctttcgc ctgaacggcg ccaaacgctt catctccaac gcgggcgtgt cggatcgcat
     1741 caccgtgttc gctacacgcg acccggccga gcgctccaag gggatctcgg ccttcatcgt
     1801 ccacgacgac gacgccggct tctcggtggg gaagtccgag aagaagatgg ggatccgcgg
     1861 atcgcccacg cgcgagctgt acttcgacaa cgtagagatc cccgaggacc gcctgatcgg
     1921 cgaggagggc aaggggttct cctacgcgat gcggacgctc gactactcca ggcccacgat
     1981 cgcggcccag gcgctgggga tcgcccaggg cgcgttcgag gtcgccgccc gctactcgcg
     2041 ggagcgggag cagttcggga agccgatcgg cgagctccag gggatccagt tcctgctggc
     2101 ggacatggcg atccagatcg aggcggcgag gctcctgacc tacaaggccg cggcggcctg
     2161 tgacgcgcag gatccgcagc tcacctacct ggcctcgatc gccaaggcgc acgccagcga
     2221 caccgcgatg caggtcacga ccgacgcggt gcaggtgctc gggggctacg gctacatgcg
     2281 tgagtacggg gtggagcgca tgatgcgcga cgccaagatc acccagctct acgagggcac
     2341 gaaccagatc cagcgcgtgg tcatcgcccg ccagatcgcg aaggccttcg gctgatgcgg
     2401 gccaagacgc ccggcgagtc gcacgtcgag ctcgtcgatc agatgcaggt caccgacgcc
     2461 aacctcatgg gcaacgttca cggcggcgtg atcatgaaga tggtggacaa cgcggccggc
     2521 ctcgcagcga tcaagcactc ggaggggccg gtggtgaccg cggcgatgga cgagatgtcg
     2581 ttcatcgagc cggtctacgt gggcgacgtc gtcacggtga aggcgacggt gaacgaggcg
     2641 ttcacgacct cgatggaggt cggggtcagg gtcgaggccg agaacctcca cacgcgcgag
     2701 gtgaagcaca cgtcgagcgc ctacctcgtc ttcgtggcca tggacgccga cggggagcca
     2761 cgaccggttc caccgctcca gccggagacc gaggaggagc ggcggcgcca gcaggaggcg
     2821 aagctccgac gcgaggcccg cctggcccgc aaggaggcga tcgaacaggc gcgagccggg
     2881 ggcgagcgag cgcctagctg aggctcgccc tgatcttctc cagggcgccg gggttctcca
     2941 ggctggagag gtcgcccggg tcctgaccgg ccaccgcggc gcggatcgcg cgccgcagga
     3001 tcttcgcgga ccgggtccgg gggagctcct ccacgaacac gatccggtcg ggcttgtagg
     3061 ccttccccag gtgctcgacg acgacgcctt gtagctcgct cgccagctcg ccctcgagct
     3121 ccgtccccgg cctcgtcacc acgaagcacc agatcgactc gcccttgacc tcgtggggga
     3181 cgccgacagc ggccgactcc gccaccaggg ggtgctcggc cagggccgat tcgacctcgg
     3241 ccggtcccag ccgtttcccg gcgatgttca tcgtgtcgtc gctccgccca tggaggaacc
     3301 agaagccgtc ctcgtccacg ctggcccagt cgccgtggac ccagacgtcg ggccagcgcg
     3361 accagtaggt gtcgaggtac cgctggggat cgccccacac cccgcgggtc atcgcgggcc
     3421 agggcttcgt gcagacgagc tcgccgacct cgccgggggg cacggacttc ccgtcggggc
     3481 cccacacctc gatcgccatg cccagggccg ggccacgcag ggtggccggc ttcagcgagt
     3541 tgatcggaag cggcgacagg aagcacgccc cgatctcggt gccacccgag aggttgatga
     3601 tcggacagcg gccgccaccg accttctcca ggaaccagag gtacgggtcc gggttccagg
     3661 gctcgccggt ggagccgagg atgcggagcg aggagaggtc gtggcgctcc acccactcgt
     3721 cgccggcggg gatcaatgcc cggatcaggg tgggggagat gccgaggtgg gtgatgcgat
     3781 ggcgctccac catgtcccag atccggtcgg gctccgggtg gttgggggcg ccctcgtaga
     3841 ggaagaccgt ggcgccgagc gcgccggcgc cggtgatctc ccagggcccc atgatccagc
     3901 ccaggtccga gacccagaac aggacgtccc cgcgctggac gtcggtctgg taggcgacct
     3961 cctcagcgat cttcacggtg aagccgccgt ggacgtggac cgagcccttg ggcctccccg
     4021 tggtgccgct cgtgtaggcg atgaagaggg ggtgctcgct gtcgaccgag agcgtctcga
     4081 acgagtgagc cgccttctcg gtcagctcct cccatctcag gtctcgatcg gctcgccatg
     4141 ggaggtcgga tctgcccagt cgggaccaga ccaccacctt ctccacggtg ggggagacgt
     4201 cggcggcctc gtcggcgatc tccttcatcg ccacctcggt cccccgccgg gggaagccgt
     4261 ccgcggtgag caggaccttg gcgtcggcgt cccggagccg ggtggccacc gcgtcggccg
     4321 cgtagcccga gaagatcggg aggtagacgg ccccgagctt ggcgcacgcg agcacggcgg
     4381 ccacggtctc gggggccatc ggcatgaaga tccccacggc gtcgcgctgg cccacgccga
     4441 gcgaggccag gccgttcgcc aggcggtcgg ccatctcgcg cagctcgcga taggtgacgg
     4501 agcggacggc gccgtcctcc ccctcccaga gcacagccac cgcgtccggc gtctcctcgg
     4561 cccaccggtc gcacgtcacg tgggccaggt tgagcttccc gccgacgaac cacttcgccc
     4621 aggggatccc ctcgctcgtg tcgagcacgc gctcgtacgg ttcgaagaat gggacgtcga
     4681 ggtcctccac gaccgcgtcc cagaaccact cgacgtcgtc ctgcgagcgg cgcacgagct
     4741 cttcgtacgt ctcgatgccg tgggcccgca tgaagcgggt gacgttcgct cgctcgatga
     4801 tctcctcggt gggctcccag acgatgtcgc tcatctgccc tcccaggtac gcttgactgc
     4861 gtgagcgagg ccaagcctac cgggaagcgg cgtctgctca ccga
//
LOCUS       DASUTZ010000179         7141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1610591, whole genome shotgun sequence.
ACCESSION   DASUTZ010000179 DASUTZ010000000
VERSION     DASUTZ010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7141
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610591"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1207
                     /locus_tag="VF097_07930"
     CDS             <1..1207
                     /locus_tag="VF097_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6262759.1"
                     /translation="PPRAPPHTMSDTATVETARPPLWRNVRVLRMAGQILFLLAVLFV
                     GREIWLNLVFGVESQNIDLSFSFLRQRAGFGIKEGIEYSANDTVTRALIVAFVNTIIV
                     AGTGIALATFFGLLIGIGRLSPNWLVRKVTQVYVEIFRNTPVLVIIIFFYFGVVLALP
                     LIGGGGIFGLAYLSNRGAAFPWPRTSEGVGLWTLLLVAAVVAATLVARWRTRVNERTG
                     EPHHRVLWWLGMFLGLGAVAYALTGAPLTIDVPDQVGRGYVGGIQISGELFGVLAGLV
                     IYTSAFIAEITRGSILAVDKGQKEAGEALGLTPFQQMRFVVLPQAMRIAIPPINSQYL
                     NLTKNTSLGLAVAYPELVSVGSTIINQKGRFTQVLIIWMLAYLTLSLAISFLMNILNR
                     IVAYRGARR"
     gene            1207..2460
                     /locus_tag="VF097_07935"
     CDS             1207..2460
                     /locus_tag="VF097_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX6262760.1"
                     /translation="MADVDEQRLQTIAAQEGPPVEVAPHARPSTPREWLRENLFSTWR
                     NGLVTVVTALVVGWALYQILRWIFVTGDFQVVKANLASYMLARFPREEVWRVWTSLYL
                     VLALAGLSWGYSERHIAWTRRRMVEATVLVLGGILVLVYLLDTVLIWMLITGAVAALL
                     VPMAAGRLLAHRYRRPLRRVVIAGWILIYPAILAILLLFDGPSSRAWGGVLLNVMAGS
                     VAIVASFPIGILLALGRRSSFPAIRTFCVGVIELIRGVPLITLLISGVFILPLLLPPQ
                     LQMPTVMRVIVMLTVFSAVYVAEIVRGGLQGVHHGQYEAARALGLSTTRMTALVILPQ
                     ALRNTIPALISHFISLWKDTSLLAIVAGLTDLLAAARRSAAGLEFIGDSKEALLGAAF
                     IFWAVAFTASRWSQRLERRVGVGER"
     gene            2526..3248
                     /locus_tag="VF097_07940"
     CDS             2526..3248
                     /locus_tag="VF097_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011430145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX6262761.1"
                     /translation="MIVAEAVEKWFGGFQALKGISTTVRRGEVVVILGPSGSGKSTFI
                     RTINRLERHDAGRIVVDGIELTDDIRNIERVRSEIGIVFQSFNLFPHMSVLHNVTLAP
                     RNVRRRPKKQAEEKAMQLLERVGIPEQAEKYPAQLSGGQQQRVAIARALAMEPKIMLF
                     DEPTSALDPEMIKEVLDVMRELARSGMTMIVVSHEIGFAKEVGDRVLFFDDGLLVEEN
                     TPERFFSRPETDRGRLFLSQIL"
     gene            3275..4138
                     /locus_tag="VF097_07945"
     CDS             3275..4138
                     /locus_tag="VF097_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HEX6262762.1"
                     /translation="MTARIIDGKAMAAEIRSEVAARVEALARRGVTPGLAAVLVGDDE
                     PSRIYVGAKQKACAEVGIHSERHELPQDASEGKLLGLILRLNEDPAVHGILVQLPLPP
                     QISTLAVHETIAVEKDVDGLTPASVGRLVRGEATYLPATPFGIVEMLVRTGIPIEGQE
                     VVMVGRGALVGMPLSIMLAQKSERGNATVTMCHTRTRDLASHTRRADILVVAAGRPGT
                     VTGDMVKPGAVVIDVAVNRTDDGLVGDVDFDGAAEVAGWITPVPGGVGPLTVAMLLVN
                     TVTAAERLAGG"
     gene            4135..5049
                     /locus_tag="VF097_07950"
     CDS             4135..5049
                     /locus_tag="VF097_07950"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEX6262763.1"
                     /translation="MTVRSDSRLERVLHEGVFAVTAEVVPPRGGDPEPVRRQARDLAG
                     YADAVNVTDNPSSSAHMSSVAGAALVAGEGLEPVVQVTVRDRNRLAITADLLGAWALG
                     ARNVLCLTGDPAAIGDHPDAVEVKDLDVLDLVRFVRTMRDEGRLLSGGPADPAPRYFV
                     GVADVPLAERYDPGRLEAKVDAGADFVQTQIVYDLEALGEWADRLRPKGIFERAFVLV
                     GVAPPRSLGSVRFMQEHLPGVVVPGSVIERLERAGHDAAAEGVRLTVEIVEGLRSIQG
                     IGGVHVMGLGREAAVRAVIEGAGLLPRP"
     gene            complement(5046..5510)
                     /locus_tag="VF097_07955"
     CDS             complement(5046..5510)
                     /locus_tag="VF097_07955"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6262764.1"
                     /translation="MKISDIMTKAAVTDRPDDTLASAAAKMWEQQTGSLLVLDGDELV
                     GIITERDVLKAVAGGTDLEATRISEVMSKDLITVDPGTSLREAAGIMTEKWIRHLPVL
                     QGGRLVGIVSQRDLSGVLAKALNEPEGLHRLIESSELVRDQRLKRIEAGDLD"
     gene            complement(5507..6625)
                     /locus_tag="VF097_07960"
     CDS             complement(5507..6625)
                     /locus_tag="VF097_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX6262765.1"
                     /translation="MTEKGLADVIAAQTSVSDIDGKLGKLWYVGYDIHDLARHSTFEE
                     TTFLLHHHRLPTAEELDQYTERLVEERELEQFTRGLFPTFGEVSAPMSMLRTAVSAAS
                     AYDPDGWDHSEEGNERKAVRLTSRIATMIATYHRFRGGEEPVPPDPKLPHAANFLLML
                     TGEEPDQEAARALDICLVLHADHTMNASTFAARVVASTLSDMHSAITAAIAALKGPLH
                     GGANERVMEMLIEIDEPEKADDHVRRLLSDGQRIMGFGHRVYKTEDPRATHLRRLSRE
                     LGERAGDTRWYEISERIEKAVMDEKGLYPNVDFYAASVYHYLGIPIDLFTTVFAASRV
                     AGWTAHVREQLSDNRLIRPESEYIGPRDQTYVPLEERR"
     gene            6720..>7141
                     /locus_tag="VF097_07965"
     CDS             6720..>7141
                     /locus_tag="VF097_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX6262766.1"
                     /translation="MAHRVTFIPGDGVGPELSEATRRVLEATGVEFDWDEQEAGADVM
                     DKYGTPLPDHVLESIRSTGAAIKGPITTPVGTGFRSVNVALRKELDLYACLRPCKWYP
                     GVRSRYEDVDLVIVRENHEDLYAGIEFEQGADDTKRLI"
BASE COUNT         1009 a         2404 c         2547 g         1181 t
ORIGIN      
        1 cccaccccgc gccccgcccc acacgatgag cgacaccgcg accgtcgaga ccgcccggcc
       61 gccgctctgg cgcaacgtcc gtgtcctgcg catggcgggc cagatcctct tcctgttggc
      121 ggtcctgttc gtcgggcggg agatctggct gaacctcgtc ttcggcgtgg agagccagaa
      181 catcgacctg tccttctcgt tcctccgaca gagggccggc ttcggtatca aggaggggat
      241 cgagtactcc gccaacgaca cggtgacccg ggcgctcatc gtggccttcg tcaacaccat
      301 catcgtggcg gggacgggga tcgccctggc cacgttcttc ggcctcctca tcgggatcgg
      361 ccgtctctcg cccaactggc tcgtgcgcaa ggtcacgcag gtctacgtcg agatcttccg
      421 caacacgccc gtgctggtca tcatcatctt cttctacttc ggggtggtgc tcgcgctgcc
      481 gctgatcggc gggggcggca tcttcgggct cgcctacctc tcgaaccgcg gcgcggcctt
      541 tccgtggcca cgaacctctg aaggcgtcgg gctgtggacg ctgctgctcg tggcggccgt
      601 cgtggccgcc acgctcgtgg cgcggtggcg cacgcgcgtc aacgagcgca ccggcgagcc
      661 gcaccaccgc gtcctctggt ggctgggcat gttcctcggg cttggcgcgg tggcctacgc
      721 cctcacgggc gcccccttga cgatcgacgt ccccgaccag gtggggaggg gctacgtagg
      781 cggcatccag atctcgggcg agctcttcgg ggtgctcgcg ggcctcgtga tctacacctc
      841 ggcgttcatc gccgagatca cccgcggcag catcctcgcg gtagacaaag gtcagaagga
      901 ggcaggggag gcgctcgggc tcacgccctt ccagcagatg aggttcgtgg tcctgccgca
      961 ggcgatgcgc atcgcgatcc cgccgatcaa cagccagtac ctgaacctga ccaagaacac
     1021 gagcctggga ctggccgtgg cctaccccga gctcgtctcg gtggggtcca ccatcatcaa
     1081 ccagaagggc cgcttcactc aggtcctcat catctggatg ctcgcctacc tgacgctcag
     1141 cctcgcgatc tcgttcctca tgaacatcct gaaccgcatc gtggcctatc ggggggcgcg
     1201 gcgctgatgg ccgacgtcga cgagcagcgg ctccagacga tcgcggccca ggagggtccc
     1261 ccggtcgagg tggcgccgca cgcccggccc tccacgccga gggagtggct ccgcgagaac
     1321 ctcttctcca cctggcggaa cgggttggtc acggtcgtca ccgcgcttgt cgtgggatgg
     1381 gctctctacc agatcctccg gtggatcttc gtgaccgggg acttccaggt ggtcaaggcc
     1441 aacctcgcct cgtacatgct cgccagattc ccccgcgagg aggtctggag ggtgtggacg
     1501 tcgctgtacc tggtgctggc cctagccggg ctgagctggg gctactccga gcgccacatc
     1561 gcctggacgc ggcgccggat ggtggaggcg acggtcctcg tgctgggggg gatcctcgtg
     1621 ctcgtctacc tcctcgacac ggtcctgatc tggatgctga tcaccggcgc ggtggcggcg
     1681 ctgctcgtcc cgatggcggc gggacgactc ctcgcccacc ggtatcgccg tcccctccgg
     1741 cgggtcgtga tcgcggggtg gatcctcatc tacccggcga tcctggcgat cctgctgctg
     1801 tttgacgggc cgtccagccg agcgtggggg ggcgtgctgc tcaacgtgat ggccgggagc
     1861 gtggcgatcg tggcctcctt cccgatcggc atcctgctcg ccctcgggcg tcggagcagc
     1921 ttcccggcga tccgtacgtt ctgcgtcggc gtgatcgagc tcatccgtgg cgtgccgctc
     1981 atcaccctgc tgatctccgg ggtgttcatc ctgccgctgc tcctgccgcc ccagctgcag
     2041 atgccgaccg tgatgcgcgt gatcgttatg ctgaccgtgt tcagcgcggt gtacgtcgcc
     2101 gagatcgtgc ggggcggcct gcagggcgtg catcatggtc agtacgaggc ggcacgcgcc
     2161 ctggggctgt ccacgacccg gatgaccgcg ctcgtgatcc tgccgcaggc tctgcgtaac
     2221 acgatcccgg ccctcatcag ccacttcatc agcctgtgga aggacacctc gctgctcgcc
     2281 atcgtggcgg gcctcaccga cctcctcgcc gcggcgcggc ggtcggcggc gggcctggag
     2341 ttcatcgggg acagcaagga ggcgctgctg ggcgcggcgt tcatcttctg ggccgtggcg
     2401 ttcacggcgt cgcggtggag ccagcgcctc gagcgacggg tgggagtggg agagcgatga
     2461 acgacgacga gcgggtggag atggagcttg aggaggcgag ggtgatcggg gacggcgacg
     2521 accggatgat cgtggctgag gccgtcgaga agtggttcgg tggcttccaa gctctcaagg
     2581 gcatcagcac gaccgtccgg cgcggcgagg tggtcgtgat cctcgggccg tccgggtcgg
     2641 gaaagtccac cttcatccga acgatcaatc gcctggagcg gcacgatgcc ggccggatcg
     2701 tcgtcgacgg gatcgagctg accgacgaca tccgcaacat cgaacgggtc cggtccgaga
     2761 tcgggatcgt gttccagtcg ttcaacctct tcccccacat gagcgtgctg cacaacgtca
     2821 cgctcgcgcc gcggaacgtg cgcaggcggc cgaagaagca ggccgaggag aaggcgatgc
     2881 agctgctcga gcgggtcggc atcccggaac aggccgagaa gtacccggcc cagctctccg
     2941 gcgggcagca gcagcgggtg gcgatcgctc gggcgctggc gatggagccg aagatcatgc
     3001 tgttcgatga gccgacctcc gccctcgatc ccgagatgat caaggaggtc ctcgacgtga
     3061 tgcgggagct cgcccgctcc gggatgacga tgatcgtcgt cagtcatgag atcgggttcg
     3121 ccaaggaggt gggggaccgc gtgctcttct tcgacgacgg gcttctcgtc gaggagaaca
     3181 cccccgagcg cttcttcagc cgccccgaga ccgaccgcgg gcggctcttc ctctcccaga
     3241 tcctctgacg cgagtcggta ggatgggcgc cggcatgacc gcgcgcatca tcgacgggaa
     3301 ggcgatggcg gccgagatcc gctcggaggt ggccgcccgc gtcgaggctc tggccaggcg
     3361 gggcgtgacc ccggggctcg ccgccgtgct ggtcggggac gacgagccct cccgcatcta
     3421 cgtcggggcg aagcagaagg cgtgcgccga ggtggggatt cactccgagc gacacgagct
     3481 ccctcaggac gcgagcgagg ggaagctcct cggcctgatc ctgcggctga acgaggaccc
     3541 tgccgttcac ggcatcctcg tccagctgcc gctcccgccg cagatctcca ccctggccgt
     3601 gcacgagacg atcgcggtgg agaaggacgt cgacgggctc acccccgcca gcgtggggag
     3661 gctcgtccgg ggcgaggcca cgtacctgcc ggcgaccccc ttcggcatcg tggagatgct
     3721 cgtgcggacc ggcatcccca tcgagggcca ggaggtcgtg atggtggggc gaggagcact
     3781 cgtggggatg cccctctcga tcatgctcgc ccagaagtcc gagcgcggga acgccaccgt
     3841 gacgatgtgc cacacgcgga cccgcgacct cgcctcccac acgcggcggg cagacatcct
     3901 cgtcgtggcc gccggccgtc cggggacggt gacgggcgac atggtgaagc cgggggccgt
     3961 cgtgatcgac gtggccgtga atcgcaccga cgacgggctc gtgggcgacg tggacttcga
     4021 cggcgccgcg gaggtcgcgg gctggatcac gcccgtcccg ggcggggtgg gcccgctcac
     4081 cgtggcgatg ctgctcgtga acaccgtcac ggccgcggaa cggctcgccg gcggatgacg
     4141 gtccggtcgg acagccggct cgagcgcgtc ctccacgagg gcgtcttcgc ggtgacggcc
     4201 gaggtggttc cgccgcgggg aggggatccc gagcccgtgc gtcgccaggc ccgcgacctc
     4261 gcaggctacg ccgacgccgt caacgtcacc gacaacccga gctcctccgc gcacatgtcg
     4321 tcggtggcgg gggccgcgct ggtggcgggc gagggcctcg agcccgtggt ccaggtgacg
     4381 gtgcgggatc ggaaccgcct ggcgatcacg gccgacctgc tcggagcttg ggccctcggc
     4441 gcccggaacg tcctctgcct gacgggggat ccggccgcca tcggcgatca tcccgatgcc
     4501 gtcgaggtca aggatctcga cgtcttggac ctggtgcgat tcgtgaggac gatgcgggac
     4561 gaggggcggc tgctgtcggg agggcctgcg gatcccgccc cgcgatactt cgtgggcgtg
     4621 gccgacgtgc ccctggctga gcggtacgac ccgggccggc tcgaggcgaa ggtcgacgcc
     4681 ggcgcggact tcgtccagac gcagatcgtc tacgacctcg aggcgctggg ggagtgggcg
     4741 gaccggctcc gcccgaaggg gatcttcgag cgggcgttcg tgctcgtggg ggtggcgccg
     4801 ccgaggagcc tgggctcggt gcggttcatg caggagcacc tccccggcgt ggtggtgccg
     4861 ggatccgtga tcgagcggct ggagagggcc ggccacgatg ctgccgccga gggcgtgcgg
     4921 ttgacggtcg agatcgtcga gggcttgcgc tcgatccagg ggatcggggg ggtgcacgtg
     4981 atggggctcg gccgtgaggc cgccgtccgg gcggtgatcg agggggccgg cctcctgccg
     5041 cggccctagt cgaggtcgcc ggcctcgatg cgcttcaggc gctggtcgcg gacgagctcg
     5101 ctcgactcga tcaggcgatg caggccctcg ggctcgttga gcgctttggc cagcaccccg
     5161 gagaggtccc gctgcgagac gatccccacg agcctccctc cctggaggac cgggaggtgg
     5221 cggatccact tctcggtcat gatccccgcc gcctcacgca gcgaggtgcc ggggtccacc
     5281 gtgatgaggt ccttcgacat gacctccgag atccgggtgg cctcgagatc cgtccctccg
     5341 gccacggcct tcaggacgtc ccgctcggtg atgatgccca cgagctcgtc cccgtcgagc
     5401 acgagcagcg agccggtctg ctgctcccac atcttcgctg cggccgaggc cagcgtgtcg
     5461 tcggggcgat cggtcaccgc cgccttcgtc atgatgtccg agatcttcat cggcgctcct
     5521 cgagggggac gtaggtctgg tcgcgcgggc cgatgtactc ggactcgggg cggatcagcc
     5581 ggttgtccga gagctgctcg cggacgtggg ccgtccaccc ggccacgcgg ctcgccgcga
     5641 ataccgtcgt gaacaggtcg atcgggatcc ccaggtagtg gtagacggag gccgcgtaga
     5701 agtcgacgtt cgggtagagg cccttctcgt ccatcacggc cttctcgatg cgctcggaga
     5761 tctcgtacca gcgcgtgtcg ccggcccgct cgcccagctc ccgggagagt cgccgcaggt
     5821 gcgtggcgcg gggatcctcg gtcttgtaca cgcggtggcc gaagcccatg atccgctggc
     5881 cgtcgctcag cagccgccgc acgtggtcgt cggccttctc gggctcgtcg atctcgatca
     5941 gcatctccat cacgcgctcg ttcgcccccc cgtgcagggg tcccttcagc gcggcgatgg
     6001 cggcggtgat cgccgagtgc atgtccgaca gcgtcgaggc cacgaccctg gccgcgaacg
     6061 tcgaggcgtt catcgtgtgg tcggcatgca gcacgaggca gatgtcgagg gccctggcgg
     6121 cctcctggtc gggctcctcg cccgtgagca tcagcaggaa gttggccgcg tgggggagct
     6181 tcggatcggg cgggaccggc tcctccccgc ccctgaagcg gtggtaagtg gcgatcatcg
     6241 tggcgatccg cgaggtcagc ctgaccgcct tccgctcgtt gccctcctcg gagtggtccc
     6301 atccgtcggg gtcgtaggcg gaggcggccg agacggcggt ccgaagcatc gacatcggcg
     6361 cggacacctc gccgaacgtt gggaagaggc cgcgcgtgaa ctgctcgagc tcccgctcct
     6421 cgacgagcct ctcggtgtac tggtcgagct cttcggccgt cgggaggcgg tggtggtgga
     6481 ggaggaaggt ggtctcctcg aacgtcgagt gccgagccag gtcgtggatg tcgtagccga
     6541 cgtaccagag cttcccgagc ttcccgtcga tgtccgagac gctcgtctga gccgcgatga
     6601 cgtccgccag gcccttctcg gtcacggtcc ctccttgtca ggcctcgggg tccgagtcta
     6661 gcagggggcg gcttctggac cgctcccgat atcatgtgcc gaccgatcga ggaggagcca
     6721 tggcgcatcg cgtgacgttc atccccggcg acggggtcgg ccccgagctc tccgaggcca
     6781 cccgccgcgt gctcgaggcc acgggggtcg agttcgactg ggacgagcag gaggccggcg
     6841 ccgacgtcat ggacaagtac ggcaccccgc tgcccgacca cgtcctggag tcgatccgca
     6901 gcaccggcgc tgccatcaag ggcccgatca cgacgccggt gggcaccgga ttccgcagcg
     6961 tcaacgtggc cctgcggaaa gagctcgacc tctacgcgtg cctccgcccc tgcaagtggt
     7021 atccgggcgt ccgatcacgg tacgaggacg tggacctcgt catcgtccgc gagaaccacg
     7081 aggacctcta cgcggggatc gagttcgagc agggggccga cgacacgaag cgcctgatcg
     7141 a
//
LOCUS       DASUTZ010000180        15209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1482426, whole genome shotgun sequence.
ACCESSION   DASUTZ010000180 DASUTZ010000000
VERSION     DASUTZ010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 15209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15209
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482426"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VF097_07970"
     CDS             <1..888
                     /locus_tag="VF097_07970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03842 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HEX6262767.1"
                     /translation="SHVLWQDVYPVFTLMANETERIRMGPLVTNPVTRDPTVTASVLA
                     TLDDISGGRMDMGIGRGDSARRVIGKTPVSVERMEWASSLIRDLVAGKEVDYDGTPIR
                     LAWAHWELPVWIAAYGPKALRAVGRVADGLIMQLADPFILEWSLGYVREGAEEAGRDP
                     ADIKIMSAAPTFISDDRAAAREQVRWFPALVSNHVVDLVNRYAKNELPSELTDYIKAR
                     DHYDYSEHGQKGAEHADFVPDEIVDRFCVIGTVDECREKLRELQRLGVDQFNVYAMSV
                     DGPDGIIETYGREIIPELS"
     gene            920..1762
                     /locus_tag="VF097_07975"
     CDS             920..1762
                     /locus_tag="VF097_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX6262768.1"
                     /translation="MTVRAAIIQANANMPKDEAIEKHVELIGKAAAEGAQITGLQEIF
                     NGPYFPAEQDAKWYAEAEPEDGPTMTRMREVARQHKMVLVVPWYEEAQTGVYYNSAAV
                     IEADGTVLGKYRKTHIPHVGPCFWEKFYFKPGNLGYPVFETSVGKVGLIICYDRHFPE
                     IARALGLKGAELVFNPSATVESLSKHLWELEQPAHAVANGYWIAAINRVGVEEPLNTA
                     KFYGSSYFCDPRGQITAQASDSEDEVLVADVDLDLIRDVRNTWQFMRDRRPETYGELT
                     ELLP"
     gene            1759..3066
                     /locus_tag="VF097_07980"
     CDS             1759..3066
                     /locus_tag="VF097_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX6262769.1"
                     /translation="MTDGGAEELLARHKEVMPSWLLLYYDEPIELVRGEGTRVWDGAG
                     KEYLDFFGGIVTTISGHVVPKLVEALTEQAGKIAHTSTVYLIRPMVELAERLTGLAPI
                     DPPTKAFFVGSGTEAVEAALLFATSMRRSNEVIALRHSYHGRSFGAVGITGNKGWSAT
                     SFSPLNVSYALSPYCYRCPLGLEYPDCGVACAEDLRNVIETTTTGEPAAMIAEPIQGV
                     GGFVTPPPEYFGIVKSILDEFGIPFIADEVQTGFGRTGESFWGIDSFDVRPDAIVCAK
                     GLGNGMAIGAVVGRTELVDSLRGNSISTFGGNPLASTYALANLDNIEEQDLQQNAYKV
                     GDYLMRGLKDLEDRYDVVGEVRGKGLMIGVELVKDGESKEPGAEEAGRVMQRAKDEGL
                     LVGKGGLYGNVLRLSPPLTITEDEAARAIETLDVTFGEIERGV"
     gene            3063..4445
                     /gene="hydA"
                     /locus_tag="VF097_07985"
     CDS             3063..4445
                     /gene="hydA"
                     /locus_tag="VF097_07985"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008737960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEX6262770.1"
                     /translation="MTMRTLIKNGTVVTAAEETAADVLIEDERIASIGIGLEAQADKV
                     IDATDRYVIPGGVDVHTHYDLPFGGSFCSDDFGTGTRAAAFGGTTTSIDFAVQDYGES
                     LRQGLDRWFEKGEKAQTDYGLHMIVREVNEGVLKEMDELVGEGVTSFKLFMAYPGVFM
                     LDDGSIFEAMQRAADSGALIMMHAENGGPIDVLVRQYLAEGKTEPVNHGLTRPAEMEG
                     EAVHRAFAMAELAGAPAYIVHLSSRDALHAVQEARDRGVAAFAETCPQYLYLSADDMA
                     RPGFEGAKFVCSPPLRPKDHQEDLWTGLAHGDLQAVATDHAPFNYKGQKDMGKDDFAK
                     IPNGLPSVEDRVTLMFQRVVEERMTPSRWVELIATNPAKMFGLYPRKGTIAPGFDADV
                     VVFDPSHERVLSAETHHMNVDYSCYEGMEVRGLPEVVIQRGNVLVEDGEWHGAEGQGR
                     FLPRSRFQEP"
     gene            4458..5954
                     /locus_tag="VF097_07990"
     CDS             4458..5954
                     /locus_tag="VF097_07990"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017551922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX6262771.1"
                     /translation="MSVTTIGSYIGGALREPGNGDVQVDPVFDPATGETIASLHHSSE
                     AEIEDAVAAAAAAFPEWANTPVPDRAQVLFRFKALLEEHLDELARLVVRENGKTLPEA
                     AGEVRRAIEVVELACGAPTLLMGTNLDQIADGIDEELVRFPVGVCAGITPFNFPNMVP
                     LWMVPVALAAGNTFVLKPSPRTPLSGMRIAELLAEAELPDGVLNVVHGGKEAVDALLT
                     HPDVSAVSFVGSVGVASYIYATAAANGKRVQALGGAKNHLIVMDDADLERSVTGLISS
                     AFGNAGQRCLAGSVAVGVGSVGDALVEELSAQASALNLGPGLEPDTDMGPVIREERRK
                     ELIGYIDEGTEVGATLALDGRGSGPSEGFFLGPTIFDGVTPDMRLWKEELFGPVLSVM
                     RASDIDEALKVLNASTYGNAASIFTSSGANAREFKRRAEAGMLGVNIGVAAPMAFFPF
                     TGWKDSFFGDLHATGADGFRFYTRQKVVTSRWSLPPALDHSQPEGRGH"
     gene            complement(5956..6028)
                     /locus_tag="VF097_07995"
     tRNA            complement(5956..6028)
                     /locus_tag="VF097_07995"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5992..5994),aa:Glu,seq:ctc)
     gene            complement(6058..6128)
                     /locus_tag="VF097_08000"
     tRNA            complement(6058..6128)
                     /locus_tag="VF097_08000"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6094..6096),aa:Gln,seq:ctg)
     gene            6263..6577
                     /locus_tag="VF097_08005"
     CDS             6263..6577
                     /locus_tag="VF097_08005"
                     /inference="COORDINATES: protein motif:HMM:NF037081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX6262772.1"
                     /translation="MNKTDLVHELVSRTDLDRKDVVRVVNAMLDVVRTTVAKGGRVTL
                     ADFGTFQRVRRAARVGRNPHTGEAVRIPATVKPMFRPGRGFLKAAAPRRRKTTRKATR
                     RR"
     gene            complement(6561..6773)
                     /locus_tag="VF097_08010"
     CDS             complement(6561..6773)
                     /locus_tag="VF097_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262773.1"
                     /translation="MAQGTIKTYDRENRTGSLLLDDETEVEIDPTSMEGSGIRYLRIG
                     QRVKFDLAEESGRRLARTLRIVTFAE"
     gene            complement(6783..7427)
                     /gene="cofC"
                     /locus_tag="VF097_08015"
     CDS             complement(6783..7427)
                     /gene="cofC"
                     /locus_tag="VF097_08015"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEX6262774.1"
                     /translation="MRVIAVPIKDLSRAKGRLSGILSPMERAALTLAMLEDVLAACVE
                     MPGWQTWVISPDPSALQQAARMRARPVEERDRGLVSAIRQVEEEAANADALAVVLADL
                     PLLTPAALMRALQTLGSVVAAPADSDAGTNLLLRRPPDAIPPRFGTDSFRKHREAAEL
                     HDIPFAEVRLPQLAFDLDRPADLTALLASSSRSRTKATCHEMGLPERLSVRKQA"
     gene            7493..8167
                     /locus_tag="VF097_08020"
     CDS             7493..8167
                     /locus_tag="VF097_08020"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX6262775.1"
                     /translation="MKRVEPWFRFAETVLIPPIALWFRWRFEGLEWVPGEGPVLVAAN
                     HISYLDPLAHGYFIEKARRRPRFLAKTELYGNPIVGAVLRGARQIPVRRGSGEHAPVE
                     SALKALRAGEVVVVYPESTVTSNADFSPMQGKTGIARLALAARVPVLPIAVWGSQRVW
                     QRGGARSLRFGRPIWVKAGPPLDLSAHEEAASDPTVLRTATQQVMDELTVLVEDLRAR
                     YPKKWA"
     gene            8164..9195
                     /locus_tag="VF097_08025"
     CDS             8164..9195
                     /locus_tag="VF097_08025"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX6262776.1"
                     /translation="MSRVAVIGAGSWGTAFARIPAEKDVETVLWARRPELAAEIGARH
                     ENPDYLPGTTLPPALTSTSDLEEAAARAEVLVVAVPSHAFREVFREAAPFLGQEVPVV
                     SLTKGIEQGSLRRMSEVMVEEGELDPGRVAVVSGPNLAKEVVAGMPSATVVACVDEER
                     AGHLQELFMAPYFRVYTNPDVIGVEMAGALKNVVAIAAGIADGMGFGDNSRASLITRG
                     LAEITRLGVKLGGAPLTFAGLAGMGDLVATCISRLSRNRTLGEALGRGRSLEEVVGEM
                     RMVAEGVKSSRPVLSLAEREGVEMPIAEHVVKVLYEGVSPADMAASLMTRAARPELHG
                     IDPGASGLG"
     gene            9206..10378
                     /locus_tag="VF097_08030"
     CDS             9206..10378
                     /locus_tag="VF097_08030"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX6262777.1"
                     /translation="MRGFTTRALGGRREDLGDAAPVAPAIHPAATYAFEDPEVLADLI
                     RRGKDVGYVYTRWHNPTRDLLERLMAELEGGERAVSFSSGMAAIVTTFAALAGSSDHV
                     VASPSLYGGSFSTLSKLLPRWGVEVTFAESHRVDEMLAPVRPETKLVYTETIGNPTLQ
                     VPDLEALGSGCRERGVPLVVDNTFASPYLCRPLELGAAVVVHSTTKYLGGHSDLTGGI
                     AVGDDELLHEVREMSVDLGGTANAWESWLTMRGIETLALRMDRHVENARALAGLLEGH
                     PAVARVWYPGLPSHPDREVAERILRAPGGMISFELAGGLESGRRFQQSLEVALVGPSL
                     GSTKTLVVHPPTVTHTQMTKEQREALGITDGFVRVSTGIEDAEDILEDFRQALEKA"
     gene            10375..11442
                     /locus_tag="VF097_08035"
     CDS             10375..11442
                     /locus_tag="VF097_08035"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEX6262778.1"
                     /translation="MIRVAVVFGGRSAEHEVSCVSAASVIGAIDRQRYEVVPIGIDKD
                     GRWMLVDGPPARDPKADRLPAIEPGMWEPVTLAGAGQPALVRSGGDRIPVDVVFPVLH
                     GPYGEDGTIQGLLEFAGIPYVGAGVLASAVAMDKSVARVLFRAAGLPLTPWLVTSRVE
                     WGEDAEAVEARAEALGVPLFVKPAALGSSVGVSKVKSLADLPAALEEAFAHGRRAVVE
                     AAVEDAREVECAILGNDEPVASVPGEIVPAGEFYDYRSKYLDDRTRLIVPADLSPETV
                     EEVQRMAVAAFQAVDAAGMARVDFLLGPRGLHLNEVNTIPGFTEVSMYPKMWEASGLP
                     YPDLIDRLIHLGLERHGGADV"
     gene            11435..12859
                     /locus_tag="VF097_08040"
     CDS             11435..12859
                     /locus_tag="VF097_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014987609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX6262779.1"
                     /translation="MSDPDPREQKEILDLAREEAQRYLDALPDEPVRTQRADEAALAF
                     DGPLPEEGVGGPAAIRELLEGSDALIRSSGPRFFHWVIGGTTPAAMGADWLTSAYDQN
                     AGAWDSSPLSARLERVAVGWLRDLFGLPQSWGGVLTTGATMANFVGLACARSWWAERH
                     GVDVDVEGLGDLPPVPVFSSGYVHPSATKALAMMGIGRERVKRLARDEAGRLDLGALE
                     RELVALGGQPAIVIGNAGEVNAGDFDPIAAMADLCEEHGSWLHVDGAFGLFAAVSPRS
                     AHLVSGIERAHSVISDGHKWLNVPHDCGFAFLREPSRLGRVFSVAAAYLPPPDEERTV
                     YFNLGPESSRRARGFTVWATLRAYGREGYRRIVERHLDLAQRLARKVEEAPDLELLAE
                     AQLNIVCFRYRPTGVPKELLDELNERIGREAMMDGRLYVGTTTYEGKAAFRPAIVNWR
                     TSEEDVDLIPDVVRELGERIRGEA"
     gene            complement(12860..13012)
                     /locus_tag="VF097_08045"
     CDS             complement(12860..13012)
                     /locus_tag="VF097_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262780.1"
                     /translation="MRACDGCRSEMRVVDSYVALEGAEEVEVIRWRCQTCGEEGESTD
                     RLLEVA"
     gene            complement(13110..13343)
                     /locus_tag="VF097_08050"
     CDS             complement(13110..13343)
                     /locus_tag="VF097_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX6262781.1"
                     /translation="MVNAYILIQTEVGKAAQVAKEVAAIDGVTSAEDVTGPYDVIVRA
                     EAGNVDDLGKLVVARIQSVDGITRTLTCPVVHL"
     gene            13428..14084
                     /locus_tag="VF097_08055"
     CDS             13428..14084
                     /locus_tag="VF097_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019631507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX6262782.1"
                     /translation="MDEGFGLPSGGFADRTEAGRLLAEALEGLEDDVVVLGIPRGGVP
                     VAAEVARARGWSLDVVIPRKIGAPGNPELGLGAIAPGVRVLDERIVARLGVPQEYLDR
                     EIERQEAEIRRRAEAYRRGRPPASVAGKTAIVVDDGVATGGTAAAALRWARAQGAEAV
                     VLAVPVGAPESIRRLSREADRVVVLAAPERFYAVGQWYRDFEQTSDEEVVRLLDEARA
                     "
     gene            14081..14374
                     /locus_tag="VF097_08060"
     CDS             14081..14374
                     /locus_tag="VF097_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262783.1"
                     /translation="MNATLDVLAWVLAGVFFLIGLRAGFDGTELEGRLSGTDRLLIAV
                     HDAARAGFWLALAGLFVGFALVDDPYAFRWFGLIPISMAALRLLAAVGLSRGR"
     gene            complement(14486..>15209)
                     /locus_tag="VF097_08065"
     CDS             complement(14486..>15209)
                     /locus_tag="VF097_08065"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF013461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEX6262784.1"
                     /translation="SAVTKPSAYLENVYQDEQGKVNFFETSYTQNGRAVFGLEDLGDY
                     ADPRDLDTVDFMLILNRNENIIPAVARLTRTQAAAYFMLGETTGTSAGGKAEEGKFLR
                     VPGTNPFFPLRQDQHGNRILELLDSHPDMEVYLMNTGRIGGKDEHEGSKKVKIPHSSA
                     VVKGIAEGTISWAEDPDFGYEVAESVPGFDDEELLQPRRLYERQGRLDEYDAMVDRLK
                     TERVEFLKRFEGLDEEIIKAVG"
BASE COUNT         2211 a         5063 c         5657 g         2278 t
ORIGIN      
        1 tcgcacgtcc tgtggcagga cgtctacccg gtcttcacgc tcatggcgaa cgagaccgag
       61 cggatccgga tgggccccct cgtgaccaac cctgtcacgc gtgatccgac ggtcaccgcc
      121 agcgtgctgg ctaccctcga cgacatctcc gggggccgca tggacatggg catcggccga
      181 ggcgacagcg cgcggcgcgt gatcgggaag acgcccgtct cggtggagcg catggagtgg
      241 gcgtcatcgc tgatccgcga cctcgtggcg ggcaaggagg tcgactacga cggcacgccg
      301 atccggctgg cgtgggcgca ctgggagctc ccggtgtgga tcgcggcgta cgggccgaag
      361 gcgctccgtg ccgtcggccg ggtcgcggac ggcctcatca tgcagctcgc cgacccgttc
      421 atcctggagt ggtcgctcgg ctacgtgcgg gagggggccg aggaggccgg ccgcgacccg
      481 gccgacatca agatcatgag cgcggccccc accttcatct cggacgaccg ggcggcagcc
      541 cgggagcagg tgcggtggtt cccggccctc gtctccaacc acgtggtgga cctcgtcaac
      601 cgctacgcga agaacgagct tccctccgag ctcaccgact acatcaaggc ccgggaccac
      661 tacgactaca gcgagcacgg ccagaagggg gccgagcacg ccgacttcgt ccccgacgag
      721 atcgtggatc gcttctgcgt gatcggcacg gtggacgaat gcagggagaa gctccgggag
      781 ctccagcggc tcggcgtgga tcagttcaac gtctacgcga tgtctgtgga cggcccggat
      841 ggcatcatcg agacgtacgg cagggagatc atccccgagc tctcctgagg gagcggggcg
      901 agtcggagag gagcgcgaca tgacggtgcg ggcggcgatc atccaggcga acgccaacat
      961 gccgaaggac gaggcgatcg agaagcacgt cgagctgatc ggcaaggccg cggcggaggg
     1021 agcccagatc acgggcctcc aggaaatctt caacggaccg tacttcccgg ccgagcagga
     1081 cgccaagtgg tacgcggagg ccgagcccga ggacgggccg accatgacgc gcatgcggga
     1141 ggtggccagg cagcacaaga tggtcctggt ggtgccctgg tacgaggagg cccagaccgg
     1201 cgtctactac aactccgcgg cggtgatcga ggccgacggc accgtgctcg ggaagtaccg
     1261 gaagacgcac atcccccacg tgggcccctg cttctgggag aagttctact tcaagcccgg
     1321 caacctgggg tatccggtct tcgagacctc ggtggggaag gtggggctga tcatctgcta
     1381 cgaccgccat ttcccggaga tcgcccgggc gctcgggctg aagggcgccg agctcgtctt
     1441 caacccctcc gccaccgtgg agtcgctctc caagcacctc tgggagctgg agcagccggc
     1501 ccacgcggtg gcgaacgggt actggatcgc cgccatcaac cgcgtggggg tcgaggagcc
     1561 cctcaacacg gcgaagttct acgggtcgtc ctacttctgc gacccgcggg gccagatcac
     1621 ggcccaggcc tcggactccg aggacgaggt gctcgtggcg gacgtcgacc tggacctgat
     1681 ccgcgacgtc cgcaacacct ggcagttcat gcgggaccgg agacccgaga cctacggcga
     1741 gctaaccgag ctgctgccat gacggacggc ggcgccgagg agctcctcgc acggcacaag
     1801 gaggtcatgc cctcctggct cctcctctac tacgacgagc cgatcgagct ggtccggggg
     1861 gaggggacca gggtgtggga cggggcgggc aaggagtacc tggacttctt cggagggatc
     1921 gtcaccacga tctccggaca cgtcgtcccg aagctcgtcg aggcgctgac cgagcaggcc
     1981 ggcaagatcg cgcacacctc cacggtgtac ctgatccggc cgatggtgga gctggccgag
     2041 cgcctcaccg ggctggcgcc gatcgacccg cccacgaagg ccttcttcgt cggatcgggg
     2101 accgaggccg tcgaggcggc cctactgttc gcgacctcga tgcgccgctc gaacgaggtg
     2161 atcgcgctcc ggcactccta ccacgggcgg tccttcgggg ccgtggggat caccggcaac
     2221 aagggctggt cggcgacctc gttctcgccg ctcaacgtgt cctacgcgct gtcgccctac
     2281 tgctaccggt gtccgctggg actcgagtat cccgactgcg gggtggcgtg cgccgaggac
     2341 ctccggaacg tgatcgagac cacgaccacc ggcgagcctg ccgcgatgat cgccgagccg
     2401 atccagggcg tcggcgggtt cgtgaccccg ccccccgagt acttcgggat cgtgaagtcg
     2461 atcctcgacg agttcgggat ccccttcatc gccgacgagg tgcagaccgg cttcggcagg
     2521 accggcgagt ccttctgggg gatcgactcc ttcgacgtcc ggcccgacgc gatcgtctgc
     2581 gccaagggtc tggggaacgg gatggccatc ggggcggtgg tgggccgcac cgagctggtg
     2641 gactccctgc gtggcaactc gatctcgacg ttcgggggga acccgctggc gtccacctac
     2701 gcgctggcca acctcgacaa catcgaggag caggacctcc agcagaacgc ctacaaggtg
     2761 ggggactacc tgatgcgcgg gctgaaggac ctcgaggatc gctacgacgt cgtgggcgag
     2821 gtccggggca agggcctcat gatcggcgtc gagctggtga aggacgggga gtcgaaggag
     2881 ccgggggccg aggaggccgg ccgggtgatg cagagggcca aggacgaggg gctgctcgtt
     2941 gggaagggcg gcctgtacgg caacgtcctg cgactctccc cgccgctcac catcaccgag
     3001 gacgaggccg cgcgggcgat cgagaccctc gacgtgacct tcggcgagat cgagcgaggg
     3061 gtgtgaccat gcggaccctc atcaagaacg gcaccgtggt gacggccgcc gaggagaccg
     3121 ccgccgacgt cctgatcgag gacgagagga tcgcctccat cgggatcggg ctcgaggccc
     3181 aggccgacaa ggtgatcgac gccaccgatc gctacgtgat cccgggcggc gtggacgtcc
     3241 acacgcacta cgacctcccc ttcggggggt cgttctgctc ggacgacttc gggacgggga
     3301 cccgggccgc ggcgttcggg gggaccacca cctcgatcga cttcgccgtg caggactacg
     3361 gagagtcgct ccgccagggc ctcgaccgct ggttcgagaa gggcgagaag gcccagaccg
     3421 actacggcct gcacatgatc gtccgcgagg tcaacgaggg cgtcctgaag gagatggacg
     3481 agctcgtggg cgagggggtc acctcgttca agctcttcat ggcctacccg ggcgtgttca
     3541 tgctcgacga cggatcgatc ttcgaggcca tgcagcgcgc ggccgacagc ggcgcgctca
     3601 tcatgatgca cgcggagaac ggcgggccga tcgacgtcct ggtcaggcag tacctggccg
     3661 aggggaagac cgagcccgtc aaccacggcc tcacgcgtcc ggccgagatg gaaggcgagg
     3721 ccgtgcaccg ggcgttcgcc atggccgagc tggccggcgc ccccgcctat atcgttcacc
     3781 tctcctcgag ggacgctctg cacgccgtcc aggaggcgcg agaccggggg gtggcggcgt
     3841 tcgccgagac gtgccctcag tacctctacc tgagcgccga cgacatggcg cgccccggct
     3901 tcgagggagc gaagttcgtc tgctccccgc cgctccgccc caaggaccac caggaggacc
     3961 tgtggaccgg gctggcccac ggcgacctgc aggccgtggc caccgaccac gcgcccttca
     4021 actacaaggg ccagaaagac atgggaaagg atgacttcgc caagatcccc aacggtctcc
     4081 ccagcgtcga ggatcgcgtc accctgatgt tccagcgcgt ggtcgaggag aggatgacgc
     4141 cctcgcgctg ggtcgagctg atcgccacca acccagcgaa gatgttcggc ctctatccgc
     4201 ggaaggggac gatcgctccc gggttcgatg ccgacgtggt cgtgttcgac ccgagccacg
     4261 agcgggtcct ctcggccgag acgcaccaca tgaacgtgga ctactcctgc tacgagggga
     4321 tggaggttag gggcctcccc gaagtcgtca tccagcgggg aaacgtcctg gtggaggacg
     4381 gggagtggca cggcgcggaa ggtcagggaa gattccttcc gcgcagcaga ttccaggagc
     4441 cgtgaagagg tgatgacatg agcgtcacga cgatcgggag ctacatcggc ggcgcgctcc
     4501 gcgagccggg gaacggggac gtccaggtgg atccggtgtt cgacccggcc acgggcgaga
     4561 cgatcgcctc ccttcaccac tccagcgagg ccgagatcga ggacgcggtg gctgccgctg
     4621 cggcggcgtt ccccgagtgg gccaacacgc ccgtgccgga ccgggcccag gtcctgttcc
     4681 ggttcaaggc cctgctggag gagcatctcg acgagctggc ccggctcgtc gtccgggaga
     4741 acggcaagac cctgcccgag gcggccggcg aggttcggcg agcgatcgag gtggtggagc
     4801 tggcctgcgg ggcaccgacc ctcctcatgg gcacgaacct ggaccagatc gccgacggga
     4861 tcgacgagga gctggtccgc ttcccggtgg gggtgtgcgc ggggatcacc ccgttcaact
     4921 tccccaacat ggtgccgctc tggatggtcc cagtggcgct ggccgccggg aacacgttcg
     4981 tgctgaagcc ctcgccccgg accccgctct cggggatgcg gatcgccgag ctgctggcgg
     5041 aggccgagct ccccgacggc gtgctgaacg tggtgcacgg cggcaaggag gcggtcgacg
     5101 ccctgctgac ccatcccgac gtgtcggcgg tctcgttcgt gggctcggtg ggggtggctt
     5161 cgtacatcta cgccacggcg gcggcgaacg ggaagcgggt ccaggcgctc ggcggcgcca
     5221 agaaccacct gatcgtgatg gacgacgccg acctcgagcg gagcgtgacc gggctgatct
     5281 cctcggcctt cgggaacgcg gggcagcggt gcctggcggg cagcgtggcc gtgggtgtgg
     5341 ggtccgtggg cgacgccctc gtcgaggagc tctcggcaca ggcctcggcg ctcaaccttg
     5401 gcccgggcct cgaacccgat accgacatgg ggccggtgat ccgggaggag cgccgcaagg
     5461 agctgattgg ctacatcgac gaagggactg aggtgggggc gacgctcgcc ctggacgggc
     5521 gcggctcggg cccgtcggag gggttctttc ttgggccgac gatcttcgac ggggtcacgc
     5581 ccgacatgcg gctgtggaag gaggagctgt tcgggccggt cctgtctgtg atgcgcgcga
     5641 gcgacatcga cgaggcgctg aaggtgctca acgcgagcac gtacggcaac gcggcctcga
     5701 tcttcacgtc gtcgggcgcg aacgcgaggg agttcaagcg gcgggccgag gcggggatgc
     5761 tcggcgtcaa tatcggcgtg gcggcgccga tggcgttctt ccccttcacg gggtggaagg
     5821 actcgttctt cggggacctg cacgcgacgg gcgcggacgg cttccgcttc tacacgaggc
     5881 agaaggtggt cacctcgagg tggtcgctgc ctccggcgct cgaccactcg cagcccgagg
     5941 gccgcggcca ctgagtgacc cctaccggat ttgaaccggt gtctccacct tgagagggtg
     6001 gtgtcctagg ccactagacg aaggggccgg ggcgccgggc tgacccggcg cggtccgctg
     6061 ggtcgcctgg attcgaacca ggactagctg atccagagtc agccgggcta ccgttacccc
     6121 acgacccatc acgtggtcga aacgaccacc tcgccatcgt agcagaggct tccgggcccg
     6181 ttccccggcc ggattgaccg gccgcgcggc cggggcgcac aatccccgcg ggccgcggtg
     6241 aacggcctcg atcggaggag ccgtgaacaa gacggacctg gtgcacgagc tggtgtctcg
     6301 gacggacttg gaccgcaagg acgtggtcag ggtcgtgaac gcgatgctcg acgtcgtccg
     6361 cacgacggtg gccaagggcg gtcgcgtcac cctggccgac ttcgggacgt ttcagcgggt
     6421 gcgtcgagcg gcgcgcgtgg gccgcaaccc gcacacgggc gaggcggtga ggatccccgc
     6481 cacggtgaag ccgatgttcc gtcccggtcg cggcttcctg aaggccgccg cgccgaggcg
     6541 gcggaagacg acgcggaagg ctactcggcg aaggtgacga tccggagcgt ccgggccagc
     6601 ctcctcccgg actcctccgc gagatcgaac ttcacccgct gaccgatccg aaggtagcgg
     6661 atccccgagc cctccatcga cgtggggtcg atctcgacct cggtctcgtc gtcgagcagc
     6721 agggatcccg tgcggttctc ccggtcgtac gtcttgatcg ttccttgggc catgcgcgct
     6781 cctcaggcct gcttccggac ggagagtctc tccgggaggc ccatctcgtg gcaagtcgcc
     6841 ttcgtccggc tgcgcgagga ggaggcgagc agggcggtca ggtccgcggg ccggtcgagg
     6901 tcgaaggcga gctgggggag ccggacctcg gcgaacggga tgtcgtggag ctcggccgcc
     6961 tcgcggtgct tcctgaagga gtcggtgccg aagcggggcg ggatcgcgtc tgggggacgg
     7021 cggagcagga ggttcgtgcc ggcgtcggag tcggccgggg cggccaccac gctgcccagc
     7081 gtttggagcg cgcgcatcag cgcggcgggc gtcaggagag ggaggtcggc cagcacgacg
     7141 gccagggcgt cggcattcgc ggcctcctcc tcgacctggc ggatcgccga gaccaggccc
     7201 cggtcgcgct cctcgacggg gcgggcccgc atccgcgcgg cctgctggag ggccgaggga
     7261 tcgggcgaga tcacccacgt ctgccagccg ggcatctcga cgcaggcggc gaggacgtct
     7321 tcgagcatgg ccagggtgag cgcggcgcgc tccatgggcg agaggatccc ggagagtcgt
     7381 cccttcgccc gggagaggtc cttgatgggg acggcgatga cgcgcatggg ggagtcatgc
     7441 tagcgggtgc gggcgttcca tggaaccatt ccccgccggt aggatggcgg ccatgaagcg
     7501 ggtcgagccc tggttccggt tcgcggagac ggtgctgatc ccgcccatcg cgctgtggtt
     7561 ccggtggcgc ttcgaggggc tggagtgggt tccgggcgag gggccggtcc tggtggcggc
     7621 caaccacatc tcctacctcg acccgttggc gcacggctac ttcatcgaga aggcgaggcg
     7681 gcgcccccgc ttcctggcga agaccgagct ctacgggaac ccgatcgtgg gggcggtgct
     7741 ccggggggcc cgtcagatcc ccgtgcggcg cgggtcgggg gagcacgcgc cggtggagtc
     7801 ggcgctgaag gcgctgcggg ccggcgaggt ggtggtcgtc tacccggagt cgaccgtgac
     7861 gagcaacgcc gacttctcgc cgatgcaggg gaagacgggc atcgctcggc tggctttggc
     7921 cgcccgtgtg cccgtcctgc cgatcgccgt ctggggctcc cagcgggtct ggcagcgggg
     7981 cggcgcgcgc agcctccgct tcggccggcc gatctgggtg aaggccgggc cccctctcga
     8041 cctttcggcc catgaggagg cggcctccga cccgaccgtg ctccggacgg cgacccagca
     8101 ggtgatggac gagctgacgg tgctcgtgga ggacctccgg gcccgatacc cgaagaagtg
     8161 ggcgtgagcc gggtcgccgt catcggggcg ggatcctggg ggacggcatt cgcgcgcatc
     8221 ccggcggaga aggacgtcga gacggtgctc tgggcccgcc gccccgagct cgccgccgag
     8281 atcggggccc gccacgagaa ccccgactat ctgccgggga cgacgctgcc tccggcgctg
     8341 acgtcgacgt cggatctcga ggaggcggcg gcgcgggccg aggtcctggt ggtggccgtc
     8401 ccgtcccacg cgttccggga ggtgttccgc gaggccgccc cgttcctcgg gcaggaagtc
     8461 cccgtggtct cgctgacgaa gggtatcgag caggggtcgc tgcggcggat gagcgaggtg
     8521 atggtcgagg agggcgagct cgatcccggc agggtggccg tggtctccgg cccgaacctg
     8581 gccaaggagg tcgtggccgg gatgccctcg gccaccgtgg tggcgtgcgt cgacgaggag
     8641 cgcgccggcc atctccagga gctgttcatg gccccgtact tccgcgtgta cacgaacccg
     8701 gacgtgatcg gggtggagat ggccggcgcg ctcaagaacg tcgtcgcgat cgccgcgggg
     8761 atcgccgacg ggatggggtt cggcgacaac tcccgcgcct cgctgatcac ccgcggcctg
     8821 gccgagatca cccggctggg ggtgaagctg ggcggagccc cgctcacgtt cgccggcctc
     8881 gccgggatgg gcgacctggt ggccacgtgc atctctcggt tgtcccggaa ccgcacgctg
     8941 ggcgaggccc tgggccgcgg ccggagcctg gaggaggtcg tgggggagat gcggatggtg
     9001 gccgagggcg tgaagtcgag ccggccggtg ctgtcgctgg ccgagcggga gggggtggag
     9061 atgccgatcg ccgagcacgt ggtgaaggtc ctgtacgagg gggtctcgcc cgcggacatg
     9121 gccgcctcgc tgatgacgcg ggcggcgcgc cccgagctcc acggcatcga cccgggagcc
     9181 tctggcctcg ggtaggcttc ccgggatgcg aggcttcacc acgcgagcgc tgggcgggcg
     9241 gcgcgaggac ctgggggacg ccgcccccgt cgccccggcg atccaccccg ccgccaccta
     9301 tgccttcgag gatcccgagg tcctggccga cctgatccgc cgtgggaagg acgtcggcta
     9361 cgtctacacg cggtggcaca accccacccg cgacctcctc gagcgcctga tggccgagct
     9421 ggagggcggc gagcgggccg tctcgttctc gtccggcatg gcggccatcg tcacgacctt
     9481 cgccgccctt gccggtagtt ccgaccacgt cgtggcgagc ccctcgctgt acggcgggag
     9541 cttctccacc ctttccaagc tgctcccccg gtggggggtc gaggtgacgt tcgcggagag
     9601 ccaccgggtc gacgagatgc ttgcccccgt gcggcccgag accaagctcg tgtacaccga
     9661 gacgatcggg aacccgacgc tgcaggtgcc cgacctcgag gccctcggct cggggtgccg
     9721 ggagcgcggg gtgccgctcg tcgtggacaa cacgttcgcc agcccctacc tctgccgtcc
     9781 gctggagctc ggggccgcgg tcgtcgtgca ctcgaccacg aagtacctgg ggggccactc
     9841 cgacctcacg ggagggatcg cggtgggcga cgacgagctg ctgcacgagg tccgcgagat
     9901 gtcggtcgac ctcggaggca ccgcgaacgc ctgggagtcg tggctcacga tgcgcggcat
     9961 cgagacgctc gccctccgga tggatcgtca cgtggagaac gcccgggcgc tggccggcct
    10021 cctggagggg caccccgccg tggcgcgcgt ctggtacccg ggcctgccgt cgcaccccga
    10081 ccgtgaggtc gccgagcgga tccttcgggc gccgggcggg atgatctcgt tcgagctggc
    10141 cgggggcctc gagtcgggcc ggaggttcca gcagtcgctg gaggtcgcgc tcgtcgggcc
    10201 cagcctgggg agcacgaaga cgctcgtcgt ccacccgccg accgtcaccc acacccagat
    10261 gacgaaggag cagcgcgagg ccctgggcat caccgacggc ttcgtccgcg tgtccacggg
    10321 gatcgaggac gccgaggaca tcctggagga cttccgccaa gcgctggaga aggcgtgatc
    10381 cgcgtcgccg tcgtcttcgg cgggcggtcc gcggagcacg aggtctcgtg cgtctcggct
    10441 gcctccgtga tcggcgcgat cgaccgccag cgctacgagg tcgtgcccat cggcatcgac
    10501 aaggacggcc gctggatgct cgtggacggc cccccggcca gggatccgaa ggccgatcgg
    10561 ctgccggcga tcgagccggg gatgtgggag cccgtgacgc tcgccggcgc gggccagccc
    10621 gccctcgtcc gctccggggg cgatcggatc ccggtggacg tcgtcttccc cgtcctccac
    10681 ggcccctacg gggaggacgg cacgatccag gggctcctgg agttcgccgg gatcccctac
    10741 gtgggggcgg gcgtgctggc ctcggcggtg gcgatggaca agtcggtggc cagggtgctg
    10801 ttccgcgccg ccggcctccc cctgacgccg tggctcgtga cctcgcgggt ggagtggggc
    10861 gaggacgccg aggccgtgga ggcccgggcc gaggcgctcg gcgtcccgct gttcgtcaag
    10921 ccggccgcgc tggggtcgtc ggtgggggtg tcgaaggtga agtccctggc ggatctcccc
    10981 gccgcgctcg aggaggcgtt cgcgcatggg cgccgcgccg tcgtggaggc ggcggtcgag
    11041 gacgcccggg aggtcgagtg cgcgatcctt gggaacgacg agccggtggc ctccgtcccc
    11101 ggggagatcg tgcccgccgg ggagttctac gactaccgtt ccaagtacct ggacgaccgc
    11161 acgcgcctga tcgtgccggc cgacctctcg cccgagacgg tggaggaggt ccagcggatg
    11221 gcggtcgccg cgttccaggc cgtggacgcc gcggggatgg cccgcgtgga ctttctcctg
    11281 gggcctcgcg gtctgcacct gaacgaggtg aacacgatcc cagggttcac cgaggtgtcg
    11341 atgtacccga agatgtggga ggcctcgggc ctcccctacc cggatctcat cgaccgcttg
    11401 atccacctgg gcctggagcg ccacggaggt gccgatgtct gaccccgatc cgcgagagca
    11461 gaaggagatc ctcgacctgg cccgggagga ggcccagcgc tacctcgacg cgcttcccga
    11521 cgagccggtg cggacccagc gagcggacga ggccgcgctg gccttcgacg gccccctccc
    11581 ggaggagggc gtgggcgggc cggccgcgat ccgcgagctc ctcgagggga gcgacgccct
    11641 gatccggtcg tccggtcccc gcttcttcca ctgggtgatc ggcgggacga ccccagcggc
    11701 gatgggggcc gactggctga cctccgccta cgaccagaac gccggggcct gggactcctc
    11761 accgctctcc gcccgcctgg agcgggtggc ggtggggtgg ctccgcgacc tcttcgggct
    11821 gccgcagagc tggggcgggg tgctcacgac gggcgcgacg atggcgaact tcgtcggcct
    11881 ggcgtgcgcc aggagctggt gggccgagcg gcacggggtc gacgttgacg tagaggggct
    11941 gggcgatctc ccgcccgtcc ccgtgttctc cagcgggtac gtgcatccca gcgccacgaa
    12001 ggcgctggcg atgatgggga tcggccggga gcgggtgaag cgcctggccc gcgacgaggc
    12061 ggggcgcctc gacctcggtg cgctggagcg ggagcttgtc gccctcggag gccagccggc
    12121 gatcgtgatc gggaacgcgg gcgaggtcaa cgcgggcgac ttcgatccga tcgccgcgat
    12181 ggccgacctc tgcgaggagc acgggtcctg gctgcacgtg gacggcgcgt tcgggctctt
    12241 cgccgcggtc tcgccgcggt ctgcgcacct cgtgagcggg atcgagcggg cccactcggt
    12301 gatctccgac gggcacaagt ggctgaacgt ccctcacgac tgcggctttg ccttcctgcg
    12361 ggagccctcc cggctgggtc gggtcttctc cgtggccgcc gcgtacctcc ccccgccgga
    12421 cgaggagcgc accgtgtact tcaacctcgg gccggagtcg tcgcgccgag ctcgaggctt
    12481 caccgtctgg gccaccctca gggcctacgg gcgcgagggc taccgcagga tcgtcgagcg
    12541 tcatctggac ttggcccagc ggctggcgag gaaggtcgag gaggctcccg atctcgagct
    12601 gctggcggag gcgcagctca acatcgtctg cttccggtac cgcccaacgg gcgtccccaa
    12661 ggagctcctg gacgagctga acgagcggat cggccgggag gcgatgatgg acggccggct
    12721 ctacgtgggg acgaccacct acgaggggaa ggcggccttc cgccccgcga tcgtgaactg
    12781 gcggacgtcc gaggaggacg tggacctcat cccggacgtc gtgagggagc tgggggagcg
    12841 gatccgcggg gaagcctagt caggccacct cgaggagccg gtcggtcgac tcgccctctt
    12901 ccccgcacgt ctggcaccgc cagcggatga cctcgacctc ctccgcccct tccagggcca
    12961 cgtaggagtc cacgacgcgc atctcgctcc ggcagccgtc acacgctcgc attcgctctc
    13021 acctccgcgt cccatcatgc cacgcgggta cgacacgaat cacacgggtg tgattatcgc
    13081 accctccccg gggaggaggt gggagcggct cagaggtgga cgaccgggca ggtcagcgtc
    13141 cgggtgatgc catccacgga ctggatccgc gccacgacga gctttccgag atcgtcgacg
    13201 ttcccggcct cggcgcgcac gatcacgtcg taggggccgg tgacgtcctc ggccgaggtg
    13261 acgccgtcga tcgcggcgac ctccttggcg acctgggcgg ccttcccgac ctcggtctgg
    13321 atcaggatgt aggcgttgac cacgtggcac ctcctgtccg tcgaagtgcc acattctacg
    13381 ccctcggccc ggcgggtcac cacggagccc cgggggataa tgcccgcatg gacgagggct
    13441 tcgggcttcc ctctggcggg ttcgccgacc ggaccgaggc cgggcgcctc ctggcggagg
    13501 ccctggaggg gctggaggac gacgtcgtgg tgctgggaat cccgcgggga ggcgtgccgg
    13561 tggcggccga ggtcgcgcgg gcccgggggt ggtcgctgga cgtggtgatc cctcggaaga
    13621 tcggggcgcc ggggaacccc gagctcgggc tgggtgcgat cgcccccggc gtgcgtgtgc
    13681 tcgacgagcg catcgtcgcc aggctgggcg tgccgcagga gtacctggat cgggagatcg
    13741 agcgccagga ggccgagatc cgaaggcggg ccgaggccta ccgccgcggc cgtcctcccg
    13801 cctccgtggc cgggaagacg gcgatcgtcg tggacgacgg cgtggccacc ggggggacgg
    13861 ccgcggcagc gctccgctgg gcccgggccc agggcgccga ggccgtggtg ctggcggtgc
    13921 ccgtcggggc tcccgagtcg atccggcggc tgtcccggga ggcagaccgg gtcgtggtcc
    13981 ttgcggcccc cgagcgcttc tacgcggtgg ggcagtggta ccgggatttc gagcagacgt
    14041 ccgacgagga ggtcgtgcgg ctcctcgacg aggcccgggc gtgaacgcga cgctggacgt
    14101 cctggcctgg gttctggccg gcgtcttctt cctgatcggc ttgagagcgg ggttcgacgg
    14161 cacggagctc gagggccggc tctcggggac cgaccgcctc ctgatcgccg tccacgacgc
    14221 ggctcgcgcc ggcttctggc tcgcgctggc cgggctcttc gtgggcttcg ccctcgtgga
    14281 cgatccctac gcgttccggt ggttcggttt gatcccgatc tcgatggcgg ccctgcgtct
    14341 gctggccgcg gtcgggctca gccgcggccg ctgagcaggc gctcgacctt caggcaccca
    14401 caggctcgtg tgcaggatgc tgatcgacgc gggcagagga gagaggtggg tgcggggcgt
    14461 ctcgggcggg ggcgccccca ccgcctcagc cgacggcctt gatgatctcc tcgtccagcc
    14521 cctcgaaccg cttcaggaac tcgacgcgct cggtcttgag gcggtccacc atcgcgtcgt
    14581 actcgtcgag gcgtccctgc cgctcgtaca gcctgcgagg ctggagcagc tcctcgtcgt
    14641 cgaacccggg gacgctctcg gccacctcgt agccgaagtc cgggtcctcg gcccacgaga
    14701 tcgttccctc ggcgatgccc ttcaccaccg cggaggaatg ggggatcttg accttcttgg
    14761 agccctcgtg ctcgtccttg cccccgatcc gccccgtgtt catcaggtag acctccatgt
    14821 cggggtgcga gtcgagcagc tcgaggatcc ggttcccgtg ctggtcctgc cgcaggggga
    14881 agaaggggtt cgtccccggc acgcggagga acttcccctc ctcggccttc ccgccggctg
    14941 aggtcccggt cgtctccccg agcatgaagt aggcggcagc ctgcgtgcgg gtgagccggg
    15001 ccacggccgg gatgatgttc tcgttccggt tgaggatcag catgaagtcg acggtatcga
    15061 ggtcgcgggg gtcggcgtag tcgccgaggt cctcgagccc gaacaccgct cgtccgttct
    15121 gcgtgtagga ggtctcgaag aagttcacct tgccctgctc gtcctggtac acgttctcga
    15181 ggtacgcgct gggcttggtg acggcgctc
//
LOCUS       DASUTZ010000181         9996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1092770, whole genome shotgun sequence.
ACCESSION   DASUTZ010000181 DASUTZ010000000
VERSION     DASUTZ010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9996
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092770"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..542
                     /gene="rpsF"
                     /locus_tag="VF097_08070"
     CDS             255..542
                     /gene="rpsF"
                     /locus_tag="VF097_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX6262785.1"
                     /translation="MREYEVMVIVDETAEDKLEAVVERVTQIVTGGGGEVTKIDRWGK
                     RKFAYEIDHKTDGFYLVIVFTADTDLLGELERVLLLADEVVRHKVVKREAA"
     gene            614..1063
                     /locus_tag="VF097_08075"
     CDS             614..1063
                     /locus_tag="VF097_08075"
                     /inference="COORDINATES: protein motif:HMM:NF005851.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX6262786.1"
                     /translation="MATENQITIVGNLTDDPELRYTPNGAAVANFRVAVSRRYRDPQT
                     NEWKDADTSFFTVNVWRSMAENAAESLTRGSRVVVVGRLKQRSWETAEGDKRTVIEIE
                     ADEVAPSLKWATAKVEKQSRGGGSGGDWNENVSVGAGGPAGETPIEG"
     gene            1080..1352
                     /gene="rpsR"
                     /locus_tag="VF097_08080"
     CDS             1080..1352
                     /gene="rpsR"
                     /locus_tag="VF097_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007319000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX6262787.1"
                     /translation="MARKQQQRQQKRRGREDRDDRKQQRKKYCYFCKEKTPIDYKDTA
                     LLRRFVSDRGKIRARRVTGNCAQHQRDVAVAVKNAREMALMPFSSR"
     gene            1349..1795
                     /gene="rplI"
                     /locus_tag="VF097_08085"
     CDS             1349..1795
                     /gene="rplI"
                     /locus_tag="VF097_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX6262788.1"
                     /translation="MKIILQKEVEKLGQPGDIVEVKDGYARNYLIPRGFATQATKGAL
                     QHAGRVRREHETRETKALEHAAAFAKRLEKTPVQVQAQAGEDGRLFGSVTAQQIAEAV
                     QRSIGEEVDRHEIRMDEPIRSTGTHEVLVHLHPEVNATITVQVVAQ"
     gene            2007..3356
                     /gene="dnaB"
                     /locus_tag="VF097_08090"
     CDS             2007..3356
                     /gene="dnaB"
                     /locus_tag="VF097_08090"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017604054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX6262789.1"
                     /translation="MARVAEEVPRPGGRVPPHNIEAEESVLGSMMLLEDAIAQVSEVL
                     QPDDFYRSAHRVIYETLIALFARGEPVDAITTVEELKRRSKLDAAGGALYIHHLVESV
                     PTPASAAHYARIVTELAMLRRLIEASARITQLAYDVPEDPVKLADQAESLIYAVARHR
                     EKEEIVPLSTLVGESLETLERMHDREGGLAGLPTGFTDLDSLLQGLQPGNLIIIAARP
                     GVGKSSLVTNIARNVSVDAATPVAMFSLEMSRIEIAMRLLCAEARVGQDRVRSMRVAA
                     DEWPRIIEAAEVLDKAPFYIVDSGAMTIVDIRAKARRLKSAQGLGLLIVDYLQLMSSH
                     QRVESRQQEIAEISRSLKLLAKELEIPVIAVSQLNRNPESRTDKRPMLADLRESGSLE
                     QDADVVMFIHREELWSDDPNVKGLAEVIVAKHRNGPTDKISLTFLSHLTQFRNFARG"
     gene            3374..4069
                     /gene="nth"
                     /locus_tag="VF097_08095"
     CDS             3374..4069
                     /gene="nth"
                     /locus_tag="VF097_08095"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX6262790.1"
                     /translation="MSTGAEAMTDVGRVREIHRRLRRAHGPLDPPRKRDPLEELVLTI
                     LSQNTSDVNRDRAFEAMRRQFPTWEEVADAPIGELRDAIRPGGLANTKAPRIQQVLRE
                     IRAREGRLDLTWMRRVGDEEVEEYLVSLPGVGPKTAACVLAFSLGRPALPVDTHVYRV
                     AGRLGLLPVGSTPGRAHQALKSLVAPAMRIPLHVGLIRHGRAICKAGVPRCDICPLAD
                     ICPSASRFLEQGA"
     gene            complement(4509..4814)
                     /locus_tag="VF097_08100"
     CDS             complement(4509..4814)
                     /locus_tag="VF097_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262791.1"
                     /translation="MRWTIRAATPAVAIVVIVVWLLGLLRSPRAVVVGVLDTVIVVVP
                     GILTRLLAVAGLLAVARLLAVSRLLAGTLVRGAGGNRALGASPQRRADPSQEGSRRG"
     gene            complement(5141..5211)
                     /locus_tag="VF097_08105"
     tRNA            complement(5141..5211)
                     /locus_tag="VF097_08105"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5177..5179),aa:Gly,seq:ccc)
     gene            complement(5249..7279)
                     /locus_tag="VF097_08110"
     CDS             complement(5249..7279)
                     /locus_tag="VF097_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020633967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX6262792.1"
                     /translation="MLGPIILLALLVVAGGIFMQRAYFLFRLVTLGRPVKRTEDLPRR
                     VEKEAVVVFGQRKLLQRIGPGLMHAFIFWGFLILLTTIIEAGGEVFDESFAIPWIGRT
                     AWLGFVQDLFAGLVLVGIGMALYFRKVRRDRRFVGSHLEEADFILVMIFGVILTLLFL
                     NATKIAMGANESPAAWTPISNVIAPLFETMTRDSQNLFRQIFLWSHIAVILGFLVYIP
                     FSKHLHILTSPLNVFFSKTKPMGKLETIDIDLENLEESRLGAATVEDLTWKQMLDTYT
                     CTECGRCQNVCPAWNTGKPLNPKLLIMNLRDHLFDHGGDILRAKKRGEEYEQVPLVPD
                     VVEEEVIWDCTTCGACMQECPVNIEHVDHIVDMRRNLVMAESSFPTEAGLLLRNLESS
                     KNPWGQSQSSRADWAEDLGVRVVEDGDAPEYLYWVGCASSFDDRSRKIARAFTRLLQK
                     AGVRFGILGPRELCNGDPARRMGNEFLFQELARENVETLNGIGTKKIVAACPHCFNTL
                     RNEYPDFGGDFEVLHHTELLAQLVDQGRLTPTEEVRALLTYHDPCYLGRHNGVYDEPR
                     SALESVPGLRTVEMERHGKRGFCCGAGGARMWMEEPIGKRVNMERTDEAAATGADILG
                     VACPYCMVMLDDGAKAKGADMQVLDVAQVLENSVFGEREPVETAAERSESSA"
     gene            7402..8034
                     /locus_tag="VF097_08115"
     CDS             7402..8034
                     /locus_tag="VF097_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262793.1"
                     /translation="MSRLRSVLGLFVTVAVLVAAGVAGAQGGLFTSDPVDEGMEEVVE
                     DPDLCAPEEGEGEEGEPEEGEPEECEPEEEPEGDEPEGDDEGEQEAEVAQAERDAACD
                     AAAGIEESPLPEGGKLAGLDNALSRVLENCKKNLQAPGLLNALERLSENRARKAARDE
                     AKAERKAEREARKAERKAAKGQGGGPGGGGPPPHAGPPGGGGPPGHAKGG"
     gene            8267..>9996
                     /gene="dnaK"
                     /locus_tag="VF097_08120"
     CDS             8267..>9996
                     /gene="dnaK"
                     /locus_tag="VF097_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017947900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX6262794.1"
                     /translation="MARAVGIDLGTTNSVVSVLEGGEPTVIPNAEGARTTPSVVGFSK
                     SGEILVGEVAKRQAITNPDRTVRSVKRHMGSKDWSVEIDDKRWTPQEISAQILTKLKR
                     DAESYLGDTVVQAVITVPAYFDDAQRQATKEAGQIAGLEVLRIINEPTAASLAYGLDK
                     EQHDQTILVFDLGGGTFDVSLLEIGEGVFEVKSTSGNTQLGGDDWDQRIIDWMVTEFK
                     NAHGVDLSKDRMALQRLKEAAEKAKIELSQVTEAQINLPFVTATNEGPLHLDLRLTRA
                     EFERMTEDLVQAAREPFEQAVKDWGKDVSEIDHIILVGGSTRMPMIQELVRDLTGGKE
                     PHKGVNPDEVVAVGAGIQAGVLKGEVKDVLLLDVTPLSLGIETKGGIFTKLIERNTTI
                     PTKKSEIFTTADDNQTQVEVHVLQGEGEMVDSRANRSLGRFHLTGIPPAPRGMPQIEV
                     TFDIDANGIVNVSAKDTATGKEQAMTITGGTALDRDELDRMMKDAEQYAEEDHRRREA
                     AEARNQADNLAYQAEKTVKELGDKVPDDDRQAVETAVADVRKALEGDDLDEIKAKTEA
                     LTEAFQKVGQA"
BASE COUNT         1787 a         3272 c         3556 g         1381 t
ORIGIN      
        1 gtctcgagga agctccgcgc cgcggcgagc gcctcgtcgg ggggcaggtc gcgtcggggg
       61 gcggggaccg tgcggtgccg agggttgcag gtcacggtcg ccttcctatc atgcggcggg
      121 agcgccggga agccccggcg atcgtgacag acccccgcgc tatggtggcg ggtgacatcg
      181 ccgtcgccgg tgctagactg cgtgagacca ctcctgctcc cccggggggc cgcgtgagtc
      241 cagaggaggt ggctttgcga gagtacgagg tcatggtcat cgtcgacgag accgccgagg
      301 acaagctcga ggcggtcgtc gagcgcgtca cccagatcgt caccggcggt ggcggcgagg
      361 tcacgaagat tgaccggtgg gggaagcgca agttcgccta cgagatcgac cacaagaccg
      421 acgggttcta cctcgtcatc gtgttcacgg ccgacaccga cctgcttggc gagctcgagc
      481 gggtgctgct gctggccgac gaggtcgtcc gtcacaaggt cgtgaagcgg gaggctgcgt
      541 agacgaggtc ttcccctcct cccggcggag gccatcggcc gggagggcga gcgaggcgag
      601 gtggtgatgc cggatggcca cggagaacca gatcacgatc gtcggcaacc tgaccgacga
      661 cccggagctg cggtacacgc ccaacggcgc ggccgtggcc aacttccgcg tggcggtgag
      721 ccgacgctac cgggacccgc agacgaacga gtggaaggac gccgacacgt cgttcttcac
      781 cgtgaacgtc tggcgctcga tggccgagaa cgccgccgag tcgctgacga ggggctcgcg
      841 ggtggtcgtc gtgggtcggc tgaagcagcg ctcctgggag accgccgagg gcgataagcg
      901 caccgtcatc gagatcgagg ccgacgaggt cgcgcccagc ctgaagtggg ccacggcgaa
      961 ggtcgagaag cagagccgcg gcggcggaag cggcggcgac tggaacgaga acgtgagcgt
     1021 cggggccggg ggaccggccg gggagacgcc gatcgagggc tgagaaggag ggaacgaagc
     1081 tggcacggaa gcagcagcag agacagcaga agaggcgggg acgggaggat cgggacgacc
     1141 gcaagcagca gcgcaagaag tactgctact tctgcaagga gaagaccccg atcgactaca
     1201 aggacacggc cctcctgcgg aggttcgtct ccgaccgggg gaagatccgg gctcgccggg
     1261 tgacgggcaa ctgcgctcag catcagcgcg acgtggccgt ggccgtgaag aacgcccgcg
     1321 agatggcgct gatgccgttc tccagccgat gaagatcatc ctccagaagg aggtcgagaa
     1381 gctcgggcag cccggcgaca tcgtcgaggt gaaggacggc tacgcgcgga actacctgat
     1441 ccctcgcggc ttcgcgaccc aggccacgaa gggggctctg cagcacgccg ggcgggtccg
     1501 cagggagcac gagacgcgcg agaccaaggc cctggagcac gccgccgctt tcgccaagcg
     1561 cctggagaag acgccggtcc aggtgcaggc gcaggccggc gaggacgggc ggctcttcgg
     1621 cagcgtgacc gcccagcaga tcgccgaggc cgtccagcgg tcgatcggcg aggaggtgga
     1681 ccgccacgag atccggatgg acgagccgat ccgctccacc ggaacgcacg aggtcctggt
     1741 ccacctccac ccggaggtca acgccacgat cacggtgcag gtcgtcgccc agtaggacat
     1801 ctcgaacccg gggtcgacgt tcgtgtcaga ccccgggttt ttcatggaac acgtgttcgg
     1861 gcatgtggaa atccgggacg caggtcacgc acggggggcg cgggcgttcc acaggttctc
     1921 cacaacgggt ccaccgcgtc cggcccttta tcacatggat accttcagcg gcgctccgct
     1981 agggtcagcc ggcgagggag ggggagatgg cacgggtcgc tgaggaggtc ccacgtccgg
     2041 gcgggcgggt cccgccgcac aacatcgagg ccgaggagtc ggtcctcggc tcgatgatgc
     2101 tcctggagga cgccatcgcc caggtcagtg aggtcctcca gcccgacgac ttctaccgct
     2161 cggcgcaccg ggtgatctac gagaccctca tcgcgctctt cgctcgtggg gagcccgtgg
     2221 acgcgatcac caccgtcgag gagttgaagc gccggagcaa gctggacgcg gcggggggcg
     2281 ccctctacat ccatcacctg gtcgagtcgg tgccgacccc cgccagcgcc gctcactacg
     2341 cccggatcgt gaccgagctg gcgatgctca ggcgcctgat cgaggccagc gcaaggatca
     2401 cccagctcgc ctacgacgtc cccgaggatc ccgtgaagct cgcggatcag gccgagagcc
     2461 tcatctacgc cgtcgctcgg catcgcgaga aggaggagat cgtcccgctc agcaccctcg
     2521 tgggcgagtc cctggagacg ctggagcgga tgcacgaccg ggagggcggc ctcgccgggc
     2581 tccccaccgg gttcaccgac ctcgactcgc tgctccaggg gctgcagccg ggcaacctga
     2641 tcatcatcgc cgccagaccg ggagtcggga aatcctccct cgtcacgaac atcgcccgca
     2701 acgtttcggt ggacgcggcc acgccggtcg ccatgttctc cctcgagatg tcccggatcg
     2761 agatcgcgat gcggctcctc tgcgccgagg cccgcgtggg tcaggaccgc gtgcgctcga
     2821 tgcgcgtggc cgccgacgag tggcccagga tcatcgaggc cgcggaggtt ctcgacaagg
     2881 cgccgttcta catcgtggac tccggcgcca tgacgatcgt cgacatcagg gccaaggccc
     2941 gccgcctcaa gtcggcgcag ggcctcggcc tcctcatcgt cgactacctc cagctcatgt
     3001 cgagccacca gcgggtggag agccgccagc aggagatcgc cgagatctcg cgctcgctga
     3061 agctcctggc caaggagctg gagatccccg tgatcgccgt ctcccaactc aaccgcaacc
     3121 cggagagccg caccgacaag cgcccgatgc tcgccgacct ccgcgagtcc ggctcgctgg
     3181 agcaagacgc cgacgtcgtg atgttcatcc acagggagga gctctggagc gacgacccga
     3241 acgtgaaggg actggccgag gtgatcgtgg ctaagcaccg gaacggcccc accgacaaga
     3301 tctcgttgac gttcctctcc cacctcacgc agttccggaa cttcgctcgc ggctagggcg
     3361 gtagggttgc agcgtgagca ccggggccga ggccatgacc gacgtcggcc gcgtccgcga
     3421 gatccatcgc cgcctgcgga gggcgcacgg cccgctcgac cctcctcgca agcgcgatcc
     3481 gctcgaggag ctcgtgctca cgatcctgtc ccagaacacc tccgacgtga accgggaccg
     3541 agccttcgag gcgatgcgtc gtcagttccc gacgtgggag gaggtggccg acgccccgat
     3601 cggggagctg cgcgacgcga tccgccccgg cggcctggcc aacacgaagg ccccgaggat
     3661 ccagcaggtt ctccgtgaga tccgggctcg cgagggaagg ctcgacctca cttggatgcg
     3721 aagggtcggt gacgaggagg tcgaggagta cctcgtgagc ctcccaggtg tgggcccgaa
     3781 gacggccgcg tgcgtcctcg ccttctccct cggacgcccg gcgcttccgg tcgataccca
     3841 cgtgtatcgg gtggccgggc ggctcggcct ccttcccgtt ggctcaaccc ccggtcgagc
     3901 gcaccaggcg ttgaagagtc tcgtggcccc ggcgatgcgc atccccctgc acgtcgggct
     3961 gatccgccac gggagggcga tctgcaaggc cggcgtgccg aggtgcgaca tctgccccct
     4021 cgcggacatc tgccccagcg cctcacggtt cctcgagcaa ggcgcctgat atggcgggaa
     4081 gtggcgaccc cccggaagcc cacgcggtcg ggctagccga ccttctctcg cgcgcgggtc
     4141 gatgcccgtc tcagaggaat cttcaccaat agggatggcg gaggcgaacg agagggtaga
     4201 ctgcgcgcag ccgggttcag ttcctcggtt ggacgtccga tagggagact aggttgcgac
     4261 ctcgatccgc gcgtgtggag ggcagcgggg cggcgcgccg ggcaggagtg accggcgcca
     4321 tcttggcgct gtcattcttg ggcaccctgg tagccctcat gatgacggct tcttctcgcc
     4381 tgcccgagcc cggccaccct ctcacgcttc caaacgagcg agaagtccag gcgatcaggg
     4441 ttcccacgcc cgacggagga gccgtcatcg ccttcgttcc cctccccagc cctcagacgg
     4501 gattcccctc atccgcggcg gctcccctcc tgggagggat cggcacgccg ctggggggag
     4561 gccccgaggg cacggttccc cccggccccc cgaacgaggg tcccggcgag gagcctggag
     4621 acggcgagga gcctggcgac ggcgaggagc ccggcgacgg cgaggagccg ggtgaggatc
     4681 ccggggacga cgacgatgac ggtgtcgaga accccgacga cgacggcccg gggcgaccgc
     4741 aggagcccga ggagccagac gacgatgacg acgatggcga cggccggggt agctgcccgg
     4801 atggtccacc tcacggcaag ggtcccggcg aggggaaggg atccgaggac gagaacggag
     4861 gtcctcccca cggaaagggc cccggggagg ggaggtgcaa ggacagccct ccccacggga
     4921 agggcaaggg acccgggaac gggaagggta aggactccgg cgggggcaac ggcggaggca
     4981 acggcggggg caacggcggg ggcaacggcg ggggcaacgg cggaggcaac ggcggaggca
     5041 acggcggagg caacggcggc gggcctccgg agggcaaggg acccgggaac ggcaagggga
     5101 agggcaacgc ccgggccaat gcgcgacgct agagggagag agcggatgag gggaatcgaa
     5161 cccccatctc gagcttggga agctcgcgtt ctaccattga actacatccg cttgagggcc
     5221 cgagtatagc gactgccgac gcgccgctct acgccgagga ttcgctcctc tcggccgccg
     5281 tctccacagg ctcccgctcc ccgaagaccg agttctccag gacctgggcc acgtccagca
     5341 cctgcatgtc ggcgcccttc gccttcgccc cgtcgtccag catcaccatg cagtaggggc
     5401 acgccacgcc gaggatgtcg gctccggtcg ccgccgcctc gtcggtgcgc tccatgttga
     5461 cccgcttgcc gatcggctcc tccatccaca tccgagcacc gccggcgcca cagcagaagc
     5521 cgcgcttccc gtggcgctcc atctccaccg tccggagccc cggcacggac tccagcgccg
     5581 agcgaggctc gtcgtagacg ccgttgtgcc gaccgaggta gcacgggtcg tgatacgtga
     5641 ggagcgcgcg gacctcttcg gtgggagtca gccggccctg atccacgagc tgggccagca
     5701 gctctgtgtg gtggagaacc tcgaagtcgc caccgaagtc cgggtactcg ttccggagcg
     5761 tgttgaagca gtggggacac gcggcgacga tctttttcgt cccgatgccg ttcagcgtct
     5821 cgacgttctc gcgggccagc tcctggaaga ggaactcgtt ccccatccga cgcgcggggt
     5881 cgccgttgca cagctcgcga ggccccagga tcccgaagcg cacgccagcc ttctgcagca
     5941 gcctcgtgaa ggcccgtgcg atcttccggg agcggtcgtc gaacgacgag gcacagccga
     6001 cccagtagag gtactcgggg gcgtcgccgt cctccaccac ccgcacgccg aggtcttccg
     6061 cccagtccgc cctcgagctc tgcgactggc cccaggggtt cttcgagctc tcgaggttgc
     6121 gcagcaggag gccggcctcg gtggggaagc tcgactccgc catgacgagg ttccggcgca
     6181 tgtccacgat gtgatcgacg tgctcgatgt tgaccgggca ctcctgcatg catgccccgc
     6241 acgtcgtgca atcccagatg acctcctcct cgacgacgtc gggaacgagc gggacctgct
     6301 cgtactcctc gcctcgtttc ttggcccgga ggatgtcgcc accgtgatcg aagaggtggt
     6361 ctcggaggtt catgatcagc agtttggggt tgagcggctt cccggtgttc caggccgggc
     6421 agacgttctg gcagcggccg cactctgtgc acgtgtaggt gtcgagcatc tgcttccagg
     6481 tgaggtcctc gacggttgcg gccccgagcc gcgactcctc gaggttctcg agatcgatgt
     6541 cgatcgtctc gagcttgccc atcggcttcg tcttggagaa gaagacgttg agggggctcg
     6601 tgaggatgtg gaggtgcttg ctgaagggga tgtagacgag gaaccccagg atcaccgcga
     6661 tgtgggacca gaggaagatc tgccggaaga ggttctgcga gtccctcgtc atcgtctcga
     6721 agagcggcgc gatgacgttc gagatcgggg tccaggccgc cggagactcg ttggctccca
     6781 tcgcgatctt cgtggcgttg aggaagagca gggtcaggat cacgccgaag atcatcacga
     6841 gtatgaagtc ggcctcctcg aggtggctgc cgacgaaccg ccggtcccgt cggaccttgc
     6901 ggaagtacag cgccatcccg atgcccacga gcaccagccc cgcgaaaagg tcctggacga
     6961 agcccagcca cgccgtgcgc ccgatccagg ggatcgcgaa gctctcgtcg aagacctcgc
     7021 cccccgcctc gatgatcgtg gtcagcagga tcaggaagcc ccagaagatg aaggcgtgca
     7081 tcagccccgg cccgatcctc tgcagcagct tccgctggcc gaagacgacg acggcttcct
     7141 tctccacacg gcggggaagg tcctcggtcc gcttgacggg gcgtcccagg gtcaccagac
     7201 ggaagaggaa gtaggcgcgc tgcatgaaga tcccgcccgc gacgacgagg agcgccagaa
     7261 ggatgatggg tccgagcacg ggccagagtc taggggccgg tccggcgcgt tcgcaacact
     7321 caccctcaag ggctcccgct cgagaaccga tacgagagag accggatcgg tggtgcatcc
     7381 gtcgtagctg gggggtaggt cgtgagcagg cttcgaagtg tcctgggatt gttcgtcacc
     7441 gtggccgtcc ttgtggcagc gggtgtggcc ggcgcccaag gcgggctctt cacctcggac
     7501 cccgtggacg aggggatgga ggaggtcgtc gaggacccgg acctttgtgc tcccgaggag
     7561 ggcgaaggcg aggaggggga gcccgaggag ggggagcccg aggagtgtga gcccgaggag
     7621 gagcccgagg gcgacgagcc cgagggcgac gacgagggcg aacaggaggc cgaggtggcg
     7681 caggccgagc gggacgccgc ctgcgacgcg gccgcgggga tcgaggagag ccccctgccg
     7741 gagggcggga agctggccgg cctcgacaac gcgctctccc gcgtcctcga gaactgcaag
     7801 aagaaccttc aggcgccggg gctgctcaac gccctggagc gcctctccga gaaccgagcc
     7861 cgcaaggccg cccgcgacga ggccaaggcc gagcgaaagg ccgagcgtga ggcccgcaag
     7921 gcagagcgga aggcggcgaa gggtcagggc gggggcccag ggggcggagg cccgcccccg
     7981 cacgcgggac ctcccggggg cggagggccc ccgggccacg ccaagggcgg ctaggggtaa
     8041 agggggctcc ccagcctccc gatagagagg ttgggcgcgg cggcgaccgc ctggctgggt
     8101 gggcgggccc tgtcagagct tcggtcgccg ccgcgcccac cgctcccaag agagccctcc
     8161 ggggctcttc ttgcctttga gccgctctgc ttgagcaaca ttgacaatat cgcgctcaag
     8221 tctctaccat gtttggcgca tccgctgagt cttcaggagg cgtgacatgg ccagagctgt
     8281 aggcatcgat ctggggacga cgaactcggt cgtctccgtg ctggagggcg gagagccgac
     8341 ggtgatcccc aacgcggagg gagcccgcac gaccccgtcc gtggtgggct tctccaagtc
     8401 cggggagatc ctcgtgggcg aggtcgccaa gcgacaggcg atcacgaacc ccgaccgcac
     8461 cgtccggtcg gtgaagcgcc acatgggttc caaggactgg agcgtcgaga tcgacgacaa
     8521 gaggtggacg ccccaggaga tctcggccca gatcctcacc aagctgaagc gggacgccga
     8581 gtcctacctc ggcgacacgg tcgtgcaggc cgtgatcacc gtccccgcgt atttcgacga
     8641 cgcgcagcgg caggccacca aggaggctgg ccagatcgcg ggcctcgagg tcctgcgcat
     8701 catcaacgag cccacggccg cgtctctcgc ctacggcctg gacaaggagc agcacgacca
     8761 gacgatcctc gtcttcgacc tgggcggcgg gaccttcgac gtctcgctgc tcgagatcgg
     8821 ggagggcgtc ttcgaggtga agtccacgag cggcaacacc cagctcgggg gggacgattg
     8881 ggaccagcgg atcatcgact ggatggtcac ggagttcaag aacgcccatg gcgtcgacct
     8941 ctccaaggat cggatggcgc tccaacggct gaaggaggcc gcggagaagg cgaagatcga
     9001 gctctcccag gtgaccgagg cccagatcaa tctgcccttc gtcaccgcca cgaacgaggg
     9061 cccgctccac ctcgatctgc ggctcacgcg ggccgagttc gagcggatga ccgaggatct
     9121 cgtgcaggcg gcccgcgagc ccttcgagca ggccgtgaag gactggggca aggacgtctc
     9181 cgagatcgac cacatcatcc ttgtcggcgg ctcgacgcgg atgcccatga tccaggagct
     9241 cgtcagggac ctcaccggag gcaaggagcc ccacaagggc gtgaacccgg acgaggtggt
     9301 ggccgtcggc gcggggatcc aggccggcgt gctcaagggc gaggtgaagg acgtcctcct
     9361 cctcgacgtg acgccactct cgctggggat cgaaaccaag ggcgggatct tcaccaagct
     9421 gatcgagcgg aacacgacga tcccaaccaa gaagtccgag atcttcacga ccgccgacga
     9481 caaccagacg caggtcgagg tgcacgtcct ccagggcgag ggcgagatgg tggactcgcg
     9541 ggccaaccgc tcgctgggcc gcttccacct gaccggcatc ccgccggcgc cgcgcggcat
     9601 gccccagatc gaggtgacct tcgacatcga cgcgaacggc atcgtcaacg tctcggcgaa
     9661 ggacacggcg acgggcaagg agcaggcgat gacgatcacc ggggggaccg ccctggacag
     9721 ggacgagctc gaccggatga tgaaggacgc ggaacagtat gcggaggagg accaccgtcg
     9781 gcgcgaagcc gccgaggcgc ggaaccaggc cgacaacctt gcctaccagg ccgagaagac
     9841 ggtcaaggag ctcggcgaca aggtccccga cgacgaccgt caggccgtgg agacggcggt
     9901 ggccgacgtc cgcaaggcgc tcgagggcga cgacctcgac gaaatcaagg cgaagaccga
     9961 ggccctgacg gaagccttcc agaaggtggg ccaggc
//
LOCUS       DASUTZ010000182         2579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1223122, whole genome shotgun sequence.
ACCESSION   DASUTZ010000182 DASUTZ010000000
VERSION     DASUTZ010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223122"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..426)
                     /locus_tag="VF097_08125"
     CDS             complement(34..426)
                     /locus_tag="VF097_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4260 domain-containing protein"
                     /protein_id="HEX6262795.1"
                     /translation="MTQPEAPSVVGLPRVLLRLEGLIVLALAILLYAKVGRSWWLFAI
                     LLLAPDLGMLGYVRGTRVGAVAYNAFHTYVAAGTLALVGVIAEHELTIALALIWFAHI
                     GFDRALGYGLKYPEGFGSTHLGRIGRRG"
     gene            complement(423..1778)
                     /locus_tag="VF097_08130"
     CDS             complement(423..1778)
                     /locus_tag="VF097_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6262796.1"
                     /translation="MGGTASKAEAAGDHQVTRSAGRPTGAVMGGFTLRFRDPYLEAAY
                     RAAHYRQNIVNIRVAHVLGALLMVAWGFAIHGHLPFWDRPFDLFIRYGVFIPVIMIGF
                     ALTFMRWYPRFWEWELLGLLFIIMPLWVYYVARLLTMPPDFGYVGLILMAMFVYTLVR
                     PRFPLVLAIAAVPIIVYVPWAVSAIQVSGVKTTIAVFYLLSFGGLGIVASHRSEQNSR
                     LLFLRERQLDRERERSDSLLLNILPKVIVARLKREPEGGRLAEGFDEVSVLFADAVRF
                     TEQAAKTSPEDLVEALDELFRRFDELSDKYGLEKIKTVGDAYMAVAGAPVPNPHHAEA
                     AAEMALGILEESSRVRWPSGDPIVMRVGVATGPAVAGVIGQRKFAYDLWGDTVNLASR
                     LEEYGEPGCILISEELAERLGDRYRLGPPKVMDLKGKGPTAVRFLMGRLGAKVPSPAD
                     A"
     gene            complement(1863..>2579)
                     /locus_tag="VF097_08135"
     CDS             complement(1863..>2579)
                     /locus_tag="VF097_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX6262797.1"
                     /translation="RTGEIELLVDRDDRAAIGMAVDHRSRLFVAGGPTGDAFVYDART
                     GAELAFYDLADGETFINDVVVTREAAWLTDSFNPFLYRIPIGPGGRLASDAEQVPLGG
                     DYQDLPGFNLNGIEATPNGRWLIAVHAAIGDLYRIDPATGEATVIDLGGDAVPNGDGI
                     LLDGKRLYVVQNFFNQIAVVDLASDLASGEIVEVIMDPRFDIPTTIAEHGHSLYAVNA
                     RFSTPPTPETEYDVVRVEKV"
BASE COUNT          430 a          905 c          869 g          375 t
ORIGIN      
        1 cgcgagagac gtcgttcgag cacggcaccc atcctatccg cggcggccga tgcggcccag
       61 gtgcgtgctt ccgaacccct ccgggtactt cagcccgtac ccgagcgcgc ggtcgaaccc
      121 gatgtgcgcg aaccagatca gagccagggc gatcgtcagc tcgtgctcgg cgatcacccc
      181 caccagggcg agggtcccgg ccgcgacgta ggtgtggaag gcgttgtacg cgacggcgcc
      241 cacccgggtg ccgcggacgt agcccagcat cccgagatcg ggggcgagca gcaggatagc
      301 gaatagccac caggagcgcc ccaccttcgc gtacagcagg atcgcaagcg cgagcacgat
      361 cagcccctcc aggcgaagga ggaccctggg gagcccgacg accgacggcg cctccggctg
      421 cgtcacgcgt cagccggcga ggggaccttc gcgcccagcc gtcccatcag gaaccggacc
      481 gctgttgggc ccttcccctt gagatccatc accttgggcg ggcccaagcg gtagcggtcc
      541 cccagacgct ctgccagctc ctcggagatc aggatgcagc cgggctcgcc gtactcctcg
      601 aggcggctgg ccaggttgac cgtgtcgccc cagaggtcgt aggcgaactt ccgctggccg
      661 atcacgccgg ccacggccgg gccggtagcc acgccgacgc gcatcacgat cgggtcgccc
      721 gagggccacc ggacacgcga ggactcctcg aggatcccga gcgccatctc ggccgccgcc
      781 tcggcgtggt gcgggttcgg gactggggct cccgccaccg ccatgtaggc gtcccccacc
      841 gtcttgatct tctccaggcc gtacttgtcg gacagctcgt cgaaccttcg gaagagctcg
      901 tcgagcgcct ccaccagatc ctcgggcgac gtcttcgcgg cctgctcggt gaacctcacc
      961 gcgtcggcga agaggacgct cacctcgtcg aacccctcgg caagcctccc cccttccggc
     1021 tcccgcttca gcctggccac gatgaccttc gggaggatgt tcaggagcag cgagtcggag
     1081 cgctcgcgct cgcgatcgag ctgtcgctcc cggaggaaca ggaggcggct gttctgctcg
     1141 ctccgatggg acgcgacgat ccccagcccc ccgaaggaca ggaggtagaa gacggcgatc
     1201 gtcgtcttca ccccggacac ctggatcgcg gagaccgccc acgggacgta gacgatgatc
     1261 ggaacggccg cgatcgccag cacgagcggg aaccgaggcc gaacgagcgt gtacacgaac
     1321 atcgccatga ggatgaggcc cacgtacccg aagtcggggg gcatcgtcag cagcctcgcg
     1381 acgtagtaga cccagagcgg catgatgatg aagaggagcc ccagcagctc ccactcccag
     1441 aagcgcgggt accagcgcat gaacgtcagc gcgaacccga tcatgatgac cgggatgaac
     1501 acgccgtacc tgatgaagag atcgaagggc cgatcccaga acggcaggtg cccgtggatc
     1561 gcgaaccccc acgccaccat caggagggcc ccgaggacgt gcgccaccct tatgttgacg
     1621 atgttctgcc ggtagtgggc ggcgcggtag gctgcctcga ggtaggggtc ccggaagcgc
     1681 agcgtgaagc ctcccatgac cgcccccgtc gggcggcccg cggaccgggt cacctggtgg
     1741 tcgccggccg cctcagcctt cgacgcggtg cctcccatga cctccatcat cctctctcgg
     1801 gagggcagcg tcgaggaaac gccggatctc gcggacgaac ctgttcgtga ggccggccgc
     1861 gctcagacct tctccaccct caccacgtcg tactcggtct cgggggtcgg cggcgtcgag
     1921 aagcgcgcgt tgaccgcgta cagcgagtgg ccgtgctccg cgatcgtcgt cgggatgtcg
     1981 aacctcgggt ccatgatgac ttccacgatc tcgccgctcg cgaggtcgga cgcgaggtcg
     2041 accaccgcga tctggttgaa gaagttctgc acgacgtaga ggcgctttcc gtcgagcagg
     2101 atcccatcgc cgttcggaac ggcatcgccc ccgaggtcga tgacggtggc ctcgcccgta
     2161 gcggggtcga tccggtagag gtcgccgatc gcggcgtgga ccgcgatcag ccaccggccg
     2221 ttcggcgtgg cctcgatccc attgaggttg aagcccggga ggtcctggta gtcgccgccc
     2281 agcggcacct gctctgcgtc ggaggccagg cggccgccgg ggccgatggg gatccggtac
     2341 aggaacgggt tgaacgagtc ggtgagccag gccgcctcgc gcgtgacgac cacgtcgttg
     2401 atgaacgtct cgccgtccgc gaggtcgtag aaggcgagct cggctcccgt gcgcgcgtcg
     2461 tagacgaagg cgtcgccggt cgggcccccg gccacgaaca gcctgctccg gtggtcgacg
     2521 gccatcccga tcgcggctcg gtcgtcccgg tccaccagca gctcgatctc gccggtgcg
//
LOCUS       DASUTZ010000183         4127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_448024, whole genome shotgun sequence.
ACCESSION   DASUTZ010000183 DASUTZ010000000
VERSION     DASUTZ010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448024"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VF097_08140"
     CDS             <1..393
                     /locus_tag="VF097_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018838437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX6262798.1"
                     /translation="EGTRTAADAARAIGCEIGQIVKSVVLMADGAPVVALTSGANRAD
                     TAKVAAIVGASRVRKADAAEAREATGFAIGGTPPFGHPRTLRVLMDRDLLGYDEVWAA
                     AGTPASVFPISPRDLVRATGAEVVELKE"
     gene            480..797
                     /locus_tag="VF097_08145"
     CDS             480..797
                     /locus_tag="VF097_08145"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX6262799.1"
                     /translation="MHHLDGIVGSALRQARERTGLSLKDVERLTNGRFKPSTVAGYER
                     GERSITVSRFVALARFYGARPSGLLSDATHAQEGRKRLVMVVPEDAAEVGDLAFAQSS
                     GEG"
     gene            complement(832..1398)
                     /locus_tag="VF097_08150"
     CDS             complement(832..1398)
                     /locus_tag="VF097_08150"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX6262800.1"
                     /translation="MAPLQGRGISLSQAFRRVVGPFPVAGEATWSNDWHAARCNPEPH
                     LHKGIDIFAPAGTPLVATRNGVVTQKGIGAVSGLHVEITGHKGIQYFYAHLSRFPPGL
                     TLGQRVSRGDVVGFVGTTGNARGTSPHVHLEVQPGGVPVPPKPFVDRWIARAELEATR
                     WVRSVLSRPTTPEEEPAAKASGPAQPRW"
     gene            2043..2537
                     /locus_tag="VF097_08155"
     CDS             2043..2537
                     /locus_tag="VF097_08155"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6262801.1"
                     /translation="MAHRDPESARFDRLAGAALRRAREAVGLTLHEVAERSGGRFKPS
                     TVAGYERGERSISLGRFAQLATVYDRPPDQLLGEVLEDIYPSVRGGVAIDESRLAFVE
                     EPAREAMRWLLRRVRERRISAESPVLTIRAGDLRVAILRSGEEPGTVLRKLRPAMVES
                     GGAS"
     gene            complement(2541..3209)
                     /locus_tag="VF097_08160"
     CDS             complement(2541..3209)
                     /locus_tag="VF097_08160"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6262802.1"
                     /translation="MSVRILLADAQSLFREAVRVVLERQGDLEIVEEAHDGLDAVAKA
                     ERAVPDVALIDASLINCDGIRATALIRERVPDCRVILLTEDEDPATLVAAMEAGASGY
                     LTKMAPLDHLAKSVRAVDRGETAIPRSMVGSLIWRLLKRRDDQDEALGLVSRLTPRER
                     EVLALLADGANNDVIAQELVISPQTARTHVHNVLQKLNVHSRLEAVAMVRRTGILGDS
                     RLAP"
     gene            complement(3442..4032)
                     /locus_tag="VF097_08165"
     CDS             complement(3442..4032)
                     /locus_tag="VF097_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6262803.1"
                     /translation="MLVASSSRLDGEAAASLLGSLRGWEVAGPLQDGLEVLGEIRRRP
                     PHAVLILHDLARLGPAPLAGQIRRRWPEVSVVVIGTVEAPHASVLPPSAARDDVLAAL
                     AAPPLDAEPVRTPVGAGMERLRTLTPRERDVLKLLGSGATRQEIASALGTSPHTVRTH
                     MHNLYRKLDAHSHLEIVRFAAQHGLIETEPGEKHKG"
BASE COUNT          560 a         1408 c         1542 g          617 t
ORIGIN      
        1 gaggggacga ggacggcggc cgacgcggct cgggcgatcg gctgcgagat cggccagatc
       61 gtgaagagcg tggtgctgat ggcggacggc gctccggtgg tcgccctcac ctcgggcgcc
      121 aaccgggcgg acacggccaa ggtcgcggcg atcgtcggcg cgtcccgggt ccggaaggcc
      181 gatgccgcgg aggcccgaga ggcgacgggt ttcgccatcg gggggacgcc cccgttcggt
      241 catcccagga cgctcagggt gctgatggat cgggacctgc tcggctacga cgaggtctgg
      301 gcggcggccg ggacgccggc ctccgtcttc ccgatctcgc cccgagacct cgtgcgggcc
      361 accggtgcgg aggtcgtgga gctgaaggag tgagtcggcc gcatcccccg gcttccccac
      421 gatggtgtgg gacggaatcg cgactccccg cgagccgatc ctccccctac catcacggta
      481 tgcaccatct ggatggaatc gtcgggtccg ccctgcggca ggctcgtgag aggaccggcc
      541 tctccctgaa ggacgtcgag cggctcacca acggacgctt caagccctcc accgtggcgg
      601 gctatgagcg aggggagcgg tcgatcacgg tatcgaggtt cgtggccctc gcccgcttct
      661 acggagcccg tccctccgga ctgttgtcag acgcaacgca cgcccaggag gggcgcaaga
      721 gactggtcat ggtcgtcccg gaggacgccg ctgaggttgg ggacctggcc ttcgcgcaga
      781 gctcgggtga gggctaggcg tccgagccgg gaggggtccg cgagtcctgg atcaccaccg
      841 aggctgagcc ggccctgagg ccttggccgc cggctcctcc tccggcgtcg tgggccggct
      901 gaggaccgac cggacccatc tcgtcgcctc gagctccgcg cgggcgatcc agcgatccac
      961 gaagggcttc gggggcaccg gcacgccgcc gggctggacc tcgaggtgca cgtgcgggga
     1021 cgtgcccctg gcgttcccgg tcgttcccac gaaccccacg acgtcacccc tcgagacgcg
     1081 ctgcccgagc gtcagtcccg gggggaaccg actgaggtgc gcgtagaagt actggatgcc
     1141 tttgtgaccc gtgatctcga cgtgcaggcc gctcaccgct ccgatgccct tctgcgtcac
     1201 gacgccattc ctcgtcgcca cgagcggggt accggcggga gcgaagatgt ctataccctt
     1261 gtggaggtgc ggctcggggt tgcagcgcgc cgcgtgccag tcgttcgacc aggtggcctc
     1321 ccccgccacg gggaaggggc ccacgacgcg gcggaacgcc tgcgagaggg agatgccccg
     1381 gccctggagc ggcgccacga tcctggcgag gcgatccgtg gtgcccggcg ggccgagatc
     1441 gggccgccgg gcttcgaggg ccagcgcacc gtcgcatccc gcgggcccgc ccttacgggc
     1501 cttcccggcc gcactctcac ccgaggaggt cgtggctgcc ggcgtccgtc cggcgccgcc
     1561 cttggagtcg ccctgctcgg gttccggctg tggcgacggc tccggctgag gcgagggagc
     1621 gggctccggc tgtggcgacg gctcgggctc gggcgcgggt ggcgggggct cttcgggtgc
     1681 gggccggttc gactggcccg gcggggattc cggcggccct tttcccggct ccgcgggcca
     1741 ggcaggagcc catgcctgcc acagaagccc tcccaccacc gtgagaagga ggagtgctcg
     1801 agtccgccgc gcgtgaagcg aatcggggaa gcgaggcccg cgcatgcccg gcagtctagg
     1861 aaccgatgcc cctgaggaga ccacgcatct gcggcgaaaa atctacgcag atctccgcag
     1921 gcggccggag tgtctgtgga cgtgcctcgt ccgtacgctc gttccccact agctgcgtag
     1981 agaatttaca gcggtacggc gtggccaggg ccctgcggtg ccatcagaat gtgtcattac
     2041 cgttggctca cagggacccg gagtcggctc gattcgaccg cctggctggg gcggccctca
     2101 ggagggctcg cgaggcggtg gggctcacgc tgcacgaggt cgccgagcga tcgggggggc
     2161 ggttcaagcc ctcgacggtg gcgggctacg agcgagggga gcgatcgatc tccctcggcc
     2221 gcttcgcgca gctggcgacg gtctacgacc ggccgcccga ccagctcctc ggtgaggtcc
     2281 tggaggacat ctacccgtcg gtccggggcg gggtcgcgat cgacgagtcc aggctggcct
     2341 tcgtggagga gcccgcccgc gaggccatga ggtggctgct gcggcgggtg cgggagaggc
     2401 ggatctccgc ggagagtccc gtgctcacga tccgggcggg cgaccttcgg gtcgcgatcc
     2461 ttcgttccgg agaggagccg gggacggtcc tgcggaagtt gcgccccgcg atggtggagt
     2521 cgggcggagc gtcgtaggtc tcagggggcg agccgcgagt cccctaggat gccggtgcgc
     2581 cggaccatgg ccaccgcctc gagccgcgag tggacgttca gcttctggag cacgttgtgc
     2641 acgtgggtgc gggctgtctg ggggctgatc accaactcct gggcgatcac gtcgttgttc
     2701 gcgccgtcgg cgaggagggc caggacctca cgctcccgcg gcgtgagccg ggacacgagc
     2761 ccgagcgcct cgtcctggtc gtccctccgc ttgaggagcc tccagatcag ggagcccacc
     2821 atgctccgag ggatcgcggt ctcaccccgg tcgacggcgc gcacgctctt cgcgaggtgg
     2881 tccagtgggg ccatcttcgt gaggtacccg ctcgcgccgg cctccatcgc ggccaccagc
     2941 gtcgccgggt cctcgtcctc ggtcaggagg atcacccgac agtccgggac ccgctctcgg
     3001 atcagcgccg tcgcccgaat cccatcgcag ttgatcaggc tggcgtcgat cagcgcgacg
     3061 tccgggacgg cccgctccgc cttggccacc gcgtcgaggc cgtcgtgcgc ctcctcgacg
     3121 atctccaggt cgccctgcct ctcgagcacg actcggacgg cctcgcggaa gagggactga
     3181 gcgtcggcca gcaggatccg gacgctcatc gtgcgttctc gggtgtcggg gtaggtgcga
     3241 tgggggacga gcataggccg ttcgtccgcg cggagcatca ggtccgcgtc gtaggccgcc
     3301 tacggcgtac ggatgagaat ctttggggca gagggtgcat aacatgacgt ctcatccgtc
     3361 cgcggtaccc cagctactcc gggcgggtgg tgccctgggt acggcatttc ggctaggatc
     3421 ccgcccggcg cggggagcgg atcagccctt gtgcttctcg cccggctcgg tctcgatcaa
     3481 gccatgctga gcggcgaaac ggacgatctc gaggtgactg tgcgcgtcga gcttccgata
     3541 gagattgtgc atgtgggtcc ggaccgtgtg ggggctggtc cccagcgcgg aggcgatctc
     3601 ctgcctcgtg gctcccgacc cgaggagctt cagcacgtcg cgctcgcggg gcgtgagcgt
     3661 ccggagccgc tccatccctg cgccgacggg cgtccgcacg ggctccgcat ccagcggggg
     3721 agcggccagc gctgcgagga cgtcatcgcg agccgccgag ggggggagga cgctcgcgtg
     3781 cggggcctcc accgtcccga tcaccaccac ggagacctcg ggccaccggc ggcggatctg
     3841 cccggcgagg ggtgccgggc ccagccgagc gaggtcgtgg aggatgagca cggcgtgagg
     3901 cgggcggcgg cggatctctc cgagcacctc gagcccgtcc tggagggggc cggccacctc
     3961 ccatccccgg agggagccga ggagcgacgc cgcggcctcc ccgtcgaggc gggaggacga
     4021 ggcgaccaac acccgccgcg tcccatccct ggggggcctc acgtcgctcc gagctccgga
     4081 ccggagatgc ggaccacctg gcccgcgtcg cgatcccccg cgtggat
//
LOCUS       DASUTZ010000184         9785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1095369, whole genome shotgun sequence.
ACCESSION   DASUTZ010000184 DASUTZ010000000
VERSION     DASUTZ010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9785
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095369"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VF097_08170"
     CDS             complement(<1..588)
                     /locus_tag="VF097_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262804.1"
                     /translation="MSGIRTGAALAVAGAATMLLLLAPTPAMAHEGREVGDVVMVVGF
                     GSEPAMAYEPNWASLTLTDHDTEQPILNGVDLEVEITYGEESTTMAMELAFGDTGEYR
                     AVFVPSRPGDYSFRFFGTVRGQGINETFSSGPDTFSPANDPAELSFPAQDPTVAEVAE
                     RLEQETTRLNESITSLEQSIAAEEPAGEEEDPAAEA"
     gene            complement(622..1164)
                     /locus_tag="VF097_08175"
     CDS             complement(622..1164)
                     /locus_tag="VF097_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262805.1"
                     /translation="MTSRRALLGLTAAGVVVGHSIAYVLAAADHAPGAHRYWPIAVAV
                     AVVAGITASVRLTLPHLREASLAPTPVLPFAARLATLQVLLFVMMEITERLAGGQSAH
                     GFLASDLAWIGVAIQVLVALAVALLTRLLGEVVGAIGRALRRVWDQPVPLPLPRALLV
                     LRPQALSGSGSMRGPPPSLD"
     gene            complement(1164..1322)
                     /locus_tag="VF097_08180"
     CDS             complement(1164..1322)
                     /locus_tag="VF097_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262806.1"
                     /translation="MPLAIVAHAGWDELLVAVVAVGVVLILRTRSERKRRLEDDREEQ
                     APEPAEEP"
     gene            1387..2388
                     /locus_tag="VF097_08185"
     CDS             1387..2388
                     /locus_tag="VF097_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase small subunit domain-containing
                     protein"
                     /protein_id="HEX6262807.1"
                     /translation="MSEVEILGVSGRDVRVTSPGKVLFPQRGITKLDLVRYYLSVGEG
                     AVHGARDRPVILHRFPHGIDGEDFYQKRVQKGAADWLVTAHIAFPSGRRAEQLVIADE
                     AHLAWMINLGCIDINPWPVRAGDLDHPDELRVDLDPTPGIPWGHVREVALLAREVLEE
                     HGLVGFVKTSGKRGIHVLVRIEPRWTFTAVRRAALALAREVERRDARATTAWWKEERT
                     GVFVDYNQNARDRTVASAYSVRPVPDARVSAPLAWDEVADVEAEDLTIDTVPGRYAEI
                     GDPHEPIDDRAGSLESLLELAERDRREGLGDAPWPPHFPKGDDEPVRAAPSRRRGPR"
     gene            complement(2385..3395)
                     /locus_tag="VF097_08190"
     CDS             complement(2385..3395)
                     /locus_tag="VF097_08190"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018893298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX6262808.1"
                     /translation="MTLPISPPLAPMLSKSRDELPRGEGWVYEPKWDGFRAIVFRDGE
                     DVHIGSRTKRPLHRYFPELLDPLRKALPERCVMDGEIVLAREGRLDFEALQLRLHPAE
                     SRVRMLSEAIPASFVGFDLPALGDEDLCARPLSERWELLAGILEERSIRDLLRDGPIV
                     ARTPRTDDPEEAERWMDELGAFGLDGVIAKTGDLAYRPGERTMVKVKKRRTADCVVGG
                     YRIHKDGQGVGSLLLALYAEDGTLHHVGFTASFRAKQRRELLDVVRPHHGESAFGEHA
                     GPGGPSRWRQEETPWIPLRPELVCEVAFNHTTGRRIRHGATFVRWREDKDPRDCTMDQ
                     IA"
     gene            3444..3638
                     /locus_tag="VF097_08195"
     CDS             3444..3638
                     /locus_tag="VF097_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262809.1"
                     /translation="MPREIDMQLFEQAVNVTATALRGAMGGEKSQPPSYAAELFREVW
                     EALKEASKDLPDRSRAGFGD"
     gene            complement(3639..4013)
                     /locus_tag="VF097_08200"
     CDS             complement(3639..4013)
                     /locus_tag="VF097_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6262810.1"
                     /translation="MQTVADVMTSGVLSVGPSTTVAEAATMMGERRVGSALVMEDDRL
                     LGIFTERDIVRALGQDFGAAERPVSEWMTPEPTTVDPETPAVEALKTMLDGGFRHLPV
                     SEGDRVLGIVSIRDLSAAGLSE"
     gene            4101..4958
                     /locus_tag="VF097_08205"
     CDS             4101..4958
                     /locus_tag="VF097_08205"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEX6262811.1"
                     /translation="MKRTWIGIVAAGLVLAACGGGATEEADGGGPVPGVAEEPAAEGL
                     QVEAGDEAVADGADVETAAAELPVLGPSVIKTAQIGLEVERGGFKDALQRATSVASAE
                     GGFVLSSSVEGKEARRGSIVLRVPSDRFEAALASLRDLGTVRRERIEGEEVGQEFVDL
                     EARLRNLRAQEAVLLRLMDEAGSIAETIRVQRQLTGIQLQVERIRGRLRYLEDRTSFG
                     TISVSLAEVGAAAPSEPSIIARAWERAAEGFLSVIGGLITALGVLTPLAVLALLAWLL
                     LRRRLPAKA"
     gene            complement(4987..6006)
                     /locus_tag="VF097_08210"
     CDS             complement(4987..6006)
                     /locus_tag="VF097_08210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6262812.1"
                     /translation="MRFGIGLFTAQVPPDSGRTFVDEYRDVVELARLAEQVGLDSVWV
                     SEHHGAGDGYMPSLLPVLGALAAVTDRVLLGTGVLLAPFHDPLRLAEDAATVDLLSEG
                     RLILGLGLGWREEEFRMWGIPIGERVRRTSETVEILRKAWTGERFSHEGHVFSYDRVR
                     ITPPPAREGGPPIYLGGFAEAPVRRAGRLGDGYIVSRSVLDVVHRNLAWAEEGAREAG
                     KDPSDLGFAILRNAFVAREGAWEAILPGVSHQLGVYPAWGAGADMPDRPFEVHPPEEE
                     TMRSVTTAGTPEEVVAGLRPFVDAFGGRRESHLILRLHYPGMDRDTAASAIELFASEV
                     LPRLR"
     gene            complement(6008..6499)
                     /locus_tag="VF097_08215"
     CDS             complement(6008..6499)
                     /locus_tag="VF097_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX6262813.1"
                     /translation="MTLDPERLLDETGWRILRELQENARITFSELGRRVALTAPAVAD
                     RVRRLEEAGVIRGYRVEVDREALGLPVLAFIRITATGAECRALAAEISGFPEVLECHR
                     VTGEEAFIAKVAVRSVAHLQDLIDRLMPYGETITSIVLSSPVSYRTIDRESANGEAAA
                     RER"
     gene            6593..7612
                     /locus_tag="VF097_08220"
     CDS             6593..7612
                     /locus_tag="VF097_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020481404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX6262814.1"
                     /translation="MARPGSMLSSPQDPSHGGGHAPTWAIWSAMWAVYIVWGSTYLAI
                     AVVVETFPPFLSAAIRFLVAGALLYAWAIRRGDGEGDRPTLRHWRSAAIVGGALLLGG
                     NGMVVWAEQTVPSGIAALLVGTVPLWMVVISGLVLRERTTWREVLGIVVGFGGVALLV
                     GPIGGGGTFDPWGVAAVVFASISWASGSLYARRAPLPERPLVGTAMEMLAGGLVLTLV
                     AAVTGEFGRVEWSEISLASWIGTAYLVVFGSLVGFAAYAWLLRVAKTSLVATYAYVNP
                     VIAVLLGWAFLREPVTARTLVAGVVIVGAVALIVSARAEHGEEEPSGELGGASVEPPD
                     EQLTA"
     gene            complement(7602..7865)
                     /locus_tag="VF097_08225"
     CDS             complement(7602..7865)
                     /locus_tag="VF097_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262815.1"
                     /translation="MAEITVEPQGDAFFVRVDDGSTQTTHTVTVSDEDFERLGSGYGS
                     REDFVSACFAFLLEHEPKESILSTFDLSVIPRYFPEFEGEIRR"
     gene            7905..9278
                     /locus_tag="VF097_08230"
     CDS             7905..9278
                     /locus_tag="VF097_08230"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX6262816.1"
                     /translation="MQATELARGIAAREISPVEVLERALLRAEHWDLVSNVFSMLHAE
                     QAQEVARVRQEEAARGEPRGPLHGVPVAVKDLFDVEGWETTGCCRAYRGAVAERDAAV
                     VARLRAAGAVIVGKTNMHELAAGGTNAVSACGACRNPWDPSRISGGSSGGSAAAVGAR
                     VVPLALGTDTGGSVRIPASMCGTVGLKTTFGSLDMMGVMPLTRTLDTVGPLTMTVEDA
                     ALGLAVIAGRGQRFVDETASPPQGLRVAVPGAFFAERARRDVLAAVHEARDVLIGMGG
                     RPATGEVGHIGYANDAWINIAWPEFAADHGLLLRRPESLYPLTRRALERGSTQPAVEM
                     VRAQRDVARLRAAFDEALAEADVLVAPATPFPAPRFEEEEIDVGSGPPHGLHTGGPAW
                     FTRVVNLVGLPALSLPAGFSDEGLPLGVQLIGRAGEEASLLRLGRAFQRATDHHLREP
                     KARSADG"
     gene            complement(9275..9706)
                     /locus_tag="VF097_08235"
     CDS             complement(9275..9706)
                     /locus_tag="VF097_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262817.1"
                     /translation="MAGREVLYETFYTKAPGSEKLRRTAEGRFRYLVREGWHEIERRP
                     SGPDSVQIRFEREGASRPLEPLRKKPEPPPRRREGRGGDRGGRGGGFRGGGGPRGGRG
                     GGPPGGQGGQPGGGQPGGGRSQGAGEGGSPEAPQTETPPSS"
BASE COUNT         1316 a         3464 c         3661 g         1344 t
ORIGIN      
        1 cgcctccgcc gcagggtcct cctcctctcc ggccggctcc tccgcggcga tcgactgctc
       61 gagcgaggtg atgctctcgt tcagcctcgt cgtctcctgc tccaggcgct cggcgacctc
      121 ggccacggtc gggtcctggg ctgggaacga gagctccgcg gggtcgttcg ccgggctgaa
      181 ggtgtcgggg ccggagctga acgtttcgtt gatcccctgg ccccggaccg tgccgaagaa
      241 ccggaacgag tagtctcccg gccgcgacgg cacgaagacg gcccggtact ccccggtgtc
      301 cccgaacgcc agctccatgg ccatcgtcgt ggactcctcc ccgtaggtga tctcgacctc
      361 gaggtccacc ccgttcagga tcggctgctc cgtgtcgtgg tctgtgagcg tcagcgaggc
      421 ccagttgggc tcgtaggcca tggccggctc gctcccgaac cccaccacca tcacgacatc
      481 ccccacctcg cgtccctcgt gggccatcgc gggcgtggga gcgagcagga gcagcatcgt
      541 ggcggcgccg gccaccgcca gcgccgcacc cgttcggatg cctgacatag gatccctccc
      601 ttcgtttcgt tggacgatgc gtcagtccag cgacggaggg gggcctcgca tcgagcccga
      661 ccccgacagc gcctgggggc ggaggacgag cagggcccgg gggaggggca gcgggacggg
      721 ctggtcccag acgcgccgca gcgcccggcc gatcgcgccg acaacctcgc ccagcaggcg
      781 ggtcagcagc gccacggcca gggcgaccag gacctggatg gcgacgccga tccacgccag
      841 gtcgctggcc aggaacccgt gggcgctctg gccgccggcc aggcgctcgg tgatctccat
      901 catcacgaag agcaggacct ggagggtggc caggcgagcg gcgaaaggca gcacgggggt
      961 gggcgcgagc gaggcctcgc gcaggtgggg gagggtcaga cggaccgagg ccgtgatccc
     1021 cgccaccacg gccaccgcca ccgcgatcgg ccagtagcgg tgagctccgg gggcgtgatc
     1081 ggccgcggcc aggacgtagg cgatcgagtg ccccaccacg acgccagccg ccgtgaggcc
     1141 gagcagggcg cgccgggagg tcatcaaggt tcctccgcgg gctcgggcgc ctgctcctcg
     1201 cggtcgtcct cgaggcggcg cttgcgctcc gaccgagtgc ggaggatcag cacgaccccc
     1261 accgcaacca cggcgacgag caactcgtcc cacccagcgt gggccacgat cgcaagaggc
     1321 acggcaatca tcctagcgcc tgtcgctgac cacaggagag gcggcccggc gtggtaggaa
     1381 tgtccggtga gcgaggtcga gatcctcggg gtctccggca gggacgtgcg cgtcaccagc
     1441 ccgggcaagg tcctcttccc ccagcggggc atcacgaagc tcgacctcgt ccgctactac
     1501 ctctcggtgg gggagggggc ggtccacggg gcccgggacc gcccggtgat cctgcaccgg
     1561 ttcccccacg ggatcgacgg cgaggacttc taccagaagc gggtgcagaa gggggcggcc
     1621 gactggctcg tcacggccca catcgcgttc cccagcggca ggagggccga gcagctggtg
     1681 atcgccgacg aggcgcacct cgcctggatg atcaacctgg ggtgcatcga catcaacccc
     1741 tggcccgtgc gggccggcga cctcgaccac ccagacgagc ttcgcgtgga cctcgacccg
     1801 acgcccggca tcccctgggg acacgtccgc gaggtggcgc tgctggctcg cgaggtgctc
     1861 gaggagcacg ggctggtggg cttcgtgaag acctcgggga agcgagggat ccacgtcctg
     1921 gtccggatcg agccgaggtg gaccttcacg gcggtccggc gcgcggcgct cgccctcgct
     1981 cgcgaggtcg agcgccgcga cgcccgggcc accaccgcct ggtggaagga ggagcgcacc
     2041 ggcgtcttcg tcgactacaa ccagaacgcg cgggaccgga cggtggcctc cgcctactcg
     2101 gtgcggcccg tccccgacgc ccgcgtctcg gcccccctgg cctgggacga ggtggccgac
     2161 gtggaggccg aggacctcac gatcgacacg gtccccggcc gctacgcgga gatcggcgac
     2221 ccgcacgagc cgatcgacga ccgagccggc tcgctggagt cgctcctcga gctcgcggag
     2281 cgcgaccgcc gcgagggcct cggcgacgcg ccgtggccgc cccacttccc caagggagac
     2341 gacgagcccg tccgggcggc cccgtcgcgt cgccgaggcc cccgctaggc gatctggtcc
     2401 atcgtgcagt cccgagggtc cttgtcctcg cgccaccgca cgaacgtcgc cccgtgacgg
     2461 atccgtcgtc ccgtcgtgtg gttgaaggcg acctcgcaca cgagctcggg gcggagcggg
     2521 atccacggcg tctcctcctg tcgccaccgg ctcggtccgc ccggcccggc gtgctcgccg
     2581 aacgcgctct ccccgtggtg gggacggacg acgtccaaca gctcccggcg ctgcttcgcc
     2641 cggaacgagg ccgtgaagcc gacgtggtgg agcgtaccgt cctcggcgta gagggcgagg
     2701 agcagcgagc ccacgccctg gccgtccttg tggatccggt agcccccgac cacgcagtcg
     2761 gcggtcctcc gcttcttgac cttcaccatg gtccgctcgc ccgggcggta ggcgaggtct
     2821 ccggtcttgg cgatcacccc gtcgagcccg aaggccccga gctcatccat ccagcgctcg
     2881 gcctcctcgg ggtcgtcggt ccgaggggtg cgggccacga tcggcccgtc ccgcagaagg
     2941 tcccggatcg atcgctcctc gaggatgccc gccaggagtt cccagcgctc ggagagcggc
     3001 cgggcgcaga ggtcctcgtc gcccagcgcc ggaaggtcga aacccacgaa cgaggccggg
     3061 atcgcctccg agagcatccg cacgcgcgac tcggcggggt ggagccgcag ctgcagggct
     3121 tcgaagtcga ggcggccctc gcgagccagc acgatctcac cgtccatgac gcaccgctcg
     3181 ggcagggcct tccgcagcgg gtcgagcagc tcggggaagt accggtggag cggacgcttc
     3241 gtcctgctcc cgatgtggac gtcctccccg tcccggaaca cgatggcccg gaagccgtcc
     3301 cacttcggct cgtagaccca gccctccccg cgcgggagct cgtcgcggga cttggacagc
     3361 atgggcgcga gtggcggcga gatcggcagg gtcacgcccg ccacgatagc cttatccgga
     3421 ccgatcacac aggaggacca gcggtgccac gcgagatcga catgcagctc ttcgagcagg
     3481 ccgtcaacgt gaccgcaacc gccctgcggg gggcgatggg cggggagaag tcccagccgc
     3541 cgtcctacgc cgccgagctc ttccgggagg tctgggaggc gctgaaggag gcctcgaagg
     3601 acctgccgga ccgctccagg gccgggttcg gggactagct actcggagag ccccgcggcc
     3661 gagaggtcgc ggatcgagac gatccccagc acgcggtcgc cctccgacac cgggaggtga
     3721 cggaacccgc cgtccaacat cgtcttgagc gcctccacgg ccggcgtctc agggtccacg
     3781 gtggtcggct cgggggtcat ccactcgctg acggggcgct ccgccgcccc gaagtcctgg
     3841 cccagcgcgc ggacgatgtc gcgctcggtg aagatcccca ggaggcggtc gtcctccatc
     3901 acgagagccg aaccgaccct gcgctcgccc atcatcgtgg ccgcctcggc caccgtggtc
     3961 gacggcccga cgctcaggac cccggacgtc atgacatccg cgaccgtctg catggccgta
     4021 ccttacttcg cgcggtgacc tgtgggaacc ccgccggagg accccccgtc caagctcccg
     4081 ggggacggag gaggtgcggg atgaagagga cgtggatcgg catcgtggcc gcagggctcg
     4141 tcctggcggc ctgcggaggg ggagcgacgg aggaggccga tggcggcggc cccgtgcccg
     4201 gggtggccga ggagccggcg gccgagggcc tgcaggtcga ggcgggggac gaggccgtcg
     4261 cggacggagc ggatgtcgag accgcggcgg ctgagctccc cgtgctgggc ccgtcggtga
     4321 tcaagacggc ccagatcggg ctcgaggtcg agcggggggg gttcaaggac gcgctccagc
     4381 gggccacctc ggtggcttcg gcggaggggg gcttcgtcct ctcctcgagc gtggagggga
     4441 aggaggcgcg gcgagggtcg atcgtgctgc gggtcccctc ggaccgcttc gaggccgcgc
     4501 tggcctcgtt gcgagacctg ggcacggtcc ggcgggagcg gatcgagggc gaggaggtgg
     4561 gccaggagtt cgtcgatctc gaggcccgtc tccgcaacct gcgggcgcag gaggctgtgc
     4621 tgctccgcct gatggacgag gccgggtcga tcgccgagac gatccgggtg cagaggcagc
     4681 tcacggggat ccagctccag gtcgagcgga tccgcgggcg gctccgctac ctcgaggatc
     4741 ggacgtcgtt cggcacgatc tcggtgtcgc tggccgaggt cggggccgcg gcgccgagcg
     4801 agccgagcat catcgcccgg gcctgggagc gggcggccga ggggttcctc tcggtgatcg
     4861 gggggctgat cacggcgctc ggggtgctga cgcccctcgc ggtcctggcc ctgctggcgt
     4921 ggctgctgct ccggcggagg ctgccggcga aggcctagcc gctcgtcgcg ccggggcgcc
     4981 cacgcgtcag cgcaggcggg ggaggacctc ggatgcgaag agttcgatcg cggaggccgc
     5041 ggtgtcccga tccatccccg gatagtggag ccggaggatg aggtgggact ccctgcggcc
     5101 gccgaaggcg tccacgaagg gccgcagccc cgcgaccact tcctcgggcg tgcccgccgt
     5161 cgtgaccgag cgcatcgtct cctcctcggg cgggtggacc tcgaagggcc ggtcgggcat
     5221 gtctgccccc gccccccagg cggggtagac gccgagctgg tgggagacgc cggggaggat
     5281 cgcctcccag gcgccctccc tggccacgaa ggcgttccgg aggatcgcga agccgaggtc
     5341 cgacgggtcc ttccccgcct cgcgcgcccc ctcctcggcc caggccaggt tgcggtggac
     5401 gacgtcgagc acggaccgcg acacgatgta gccgtccccg aggcggcccg cgcggcgcac
     5461 cggagcctcg gcgaaccccc cgaggtagat cggcgggccg ccctcccggg ccggaggggg
     5521 cgtgatcctg acccggtcgt aggagaagac gtggccctcg tggctgaagc gctcgcccgt
     5581 ccacgccttc cggaggatct ccacggtctc cgacgtgcga cgcacgcgct cgccgatggg
     5641 gatcccccac atgcggaact cctcctcgcg ccagccgagg cccagcccga ggatcagccg
     5701 cccctcggac aggaggtcca ccgtggccgc gtcctcggcc agccgcagcg ggtcgtggaa
     5761 gggcgcgagc agcacgccgg tgccgaggag gacgcggtcg gtgacggcgg cgagcgcgcc
     5821 gagcacgggg agcagggagg gcatgtagcc gtcgccggcg ccgtgatgct cgctcaccca
     5881 caccgagtcc agcccgacct gctctgcgag gcgcgcgagc tcgacgacgt ctcggtactc
     5941 gtcgacgaac gtccggcccg agtcgggcgg cacctgcgcg gtgaagagcc cgatgccgaa
     6001 ccgcacgtca gcgctcccga gcggcagcct cgccgttcgc cgactcccgg tcgatcgtcc
     6061 ggtaggacac gggcgaggag agcacgatcg aggtgatcgt ctccccgtag ggcatgaggc
     6121 ggtcgatcag gtcctggagg tgcgccaccg atcggacggc caccttggcg atgaaggcct
     6181 cctcgccggt gacgcggtgg cactcgagga cctcggggaa ccccgagatc tcggcggcca
     6241 gcgcccggca ctcggcgccc gtcgcggtga tgcggatgaa cgccagcacg gggaggccga
     6301 gggcctcgcg gtcgacctcc acccggtagc ctcggatcac cccggcctcc tcgaggcggc
     6361 gcaccctgtc ggcgaccgcc ggtgcggtga gggcgacccg cctccccagc tcgctgaagg
     6421 tgatgcgggc gttctcctgg agctcgcgga ggatccgcca gcccgtctcg tcgaggagtc
     6481 gctcaggatc caaggtcatc gcctccgttc gtcttcgtat gacaagcgtc ctcgcccgaa
     6541 gaccttagac agagggttca cagacggtca agaccctctc tatcatgggg tcatggcacg
     6601 gcccggctcg atgctctcct cccctcagga cccgtcccac ggcggcggac acgctccgac
     6661 ctgggcgatc tggtcggcga tgtgggccgt ctacatcgtg tggggctcga cgtacctggc
     6721 gatcgctgtc gtcgtggaga cgttcccgcc cttcctctcg gcggcgatcc gtttcctcgt
     6781 ggcgggcgcc ctgctctacg cgtgggcgat ccggagaggg gacggcgagg gcgaccgccc
     6841 cacgctccgc cactggcggt cggcggcgat cgtgggcggg gcgctgcttc tcggcggcaa
     6901 cgggatggtg gtgtgggccg agcagacggt gccgtccggg atcgcggccc tgctcgtcgg
     6961 gaccgtgccg ctgtggatgg tggtcatctc gggcctggtg cttcgcgagc ggacgacctg
     7021 gcgagaggtg ctggggatcg tcgtggggtt cggcggtgtg gccctgctcg tgggcccgat
     7081 cggcgggggt gggaccttcg acccgtgggg ggtggccgcg gtggtctttg cctcgatctc
     7141 gtgggcctcg ggctcgctct acgcgcgccg tgcgccgctg cccgagcgcc cgctcgtggg
     7201 gaccgcgatg gagatgctgg ccgggggcct ggttctcacc ctcgtggccg cggtcaccgg
     7261 cgagttcggt cgcgtggagt ggagcgagat ctcgctcgca tcgtggatcg gcaccgccta
     7321 cctcgtggtg ttcggctcgc tggtgggctt cgccgcctac gcctggctgc tgcgggtggc
     7381 gaagacctcg ctcgtcgcca cctacgccta cgtgaacccg gtgatcgccg tgctgctggg
     7441 ctgggccttc ctgcgcgagc cggtcacggc gcgcacgctc gtggccgggg tcgtcatcgt
     7501 gggggccgtg gcgctgatcg tgagcgccag ggccgagcac ggcgaggagg agccgtccgg
     7561 cgagcttggg ggggcctcgg tggagcctcc cgacgagcaa ctcaccgcct gatctcgccc
     7621 tcgaactccg ggaagtaccg ggggatcacg ctcaggtcga acgtcgagag gatcgactcc
     7681 ttcggctcgt gctccaggag gaacgcgaag cacgcggaca cgaagtcctc ccgcgacccg
     7741 tacccggagc caagccgttc gaagtcctcg tccgagaccg tgaccgtgtg cgtggtctgg
     7801 gtcgatccgt cgtccacacg cacgaagaag gcgtcaccct gcggctccac cgtgatctcc
     7861 gccacgaccc cacgctacca gggcgcgcgg taggcttccc gtcgatgcag gcgaccgagc
     7921 tcgctcgcgg gatcgccgct cgcgagatct cccccgtgga ggtcctggag cgagcgctgc
     7981 tgcgcgcgga gcactgggac ctcgtctcga atgtcttctc gatgctgcac gctgagcagg
     8041 cgcaggaggt ggcccgcgtt cggcaggagg aggcggcgcg cggggagccg cggggaccac
     8101 tccacggcgt gcccgtcgcc gtgaaggacc tcttcgacgt cgaggggtgg gagacgaccg
     8161 ggtgctgccg agcgtatcgg ggcgccgtgg ccgagcgcga cgccgcggtg gtggcccggc
     8221 tccgggcggc cggcgcggtg atcgtcggga agacgaacat gcacgagctc gccgcgggcg
     8281 ggacgaacgc ggtgtcggcg tgcggcgcat gccgcaaccc gtgggacccc tcgaggatca
     8341 gcggcgggtc gagcgggggc tcggcggctg ccgtgggcgc ccgggtcgtg ccgctggcgc
     8401 tggggaccga cacgggaggc tcggtgcgca tccccgcctc gatgtgcggc accgtcgggc
     8461 tgaagaccac gttcgggagc ctcgacatga tgggcgtcat gccgctgacc cgcacgctgg
     8521 acacggtggg tccgctgacg atgaccgtcg aggacgctgc cctggggctc gcggtgatcg
     8581 cggggcgggg acagcggttc gtggacgaga ccgcgtcacc gccccagggg ctgagggtgg
     8641 ccgtcccggg cgccttcttc gccgagcggg cccggcggga cgtcctggcc gcggtgcacg
     8701 aggcgcgcga cgtcctgatc gggatggggg ggaggcccgc cacgggcgaa gtgggccaca
     8761 tcggctacgc caacgatgcc tggatcaaca tcgcctggcc cgagttcgcc gcggaccacg
     8821 ggctgctgct ccgccgcccc gagtcgctgt acccgctcac gcgccgggcc ctggagcgcg
     8881 gctccaccca gccggccgtc gagatggttc gagcccagcg cgacgtggcc cggcttcgcg
     8941 cggccttcga cgaggcgctg gccgaagcgg acgtgctggt agcgccggcc acgcccttcc
     9001 cggccccgcg cttcgaagag gaggagatcg acgtggggag cggcccgccg cacggcctgc
     9061 acacgggggg cccggcgtgg ttcacgaggg tggtgaacct ggtgggcctg ccggccctct
     9121 cgctcccagc ggggttctcg gacgaggggt tgccgctcgg ggtgcagctg atcggacggg
     9181 ccggggagga ggcgtcgctc ttgaggctgg ggcgcgcgtt ccagcgcgcc accgaccatc
     9241 acctgcggga gccgaaggcg agaagcgcgg acggctagga ggaggggggg gtctcggtct
     9301 gaggcgcctc gggggacccg ccctcgccgg cgccctgtga ccgtccgccc ccgggctggc
     9361 cgcccccggg ctggccgccc tgcccgcccg gggggccgcc gccgcggccg ccgcgagggc
     9421 cgccgcctcc ccggaacccg ccgcctcgac cgccacggtc gccacctcgg ccctcgcggc
     9481 ggcgcggcgg cggctcgggc ttcttccgca ggggctcgag cgggcggctc gcgccctcgc
     9541 gctcgaaccg gatctggacc gagtcggggc ccgagggccg ccgctcgatc tcgtgccatc
     9601 cctcgcggac gagatagcgg aaccgcccct cggcggtcct ccgcagcttc tcggagcccg
     9661 gcgccttggt gtagaaggtc tcgtagagga cttctctgcc tgccatgtgg gttcctaacg
     9721 aggccggacg gcctccggcg ggcaactgta gcacacggcc gcgggacctc ccgcgcccga
     9781 ggaag
//
LOCUS       DASUTZ010000185         8301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1743601, whole genome shotgun sequence.
ACCESSION   DASUTZ010000185 DASUTZ010000000
VERSION     DASUTZ010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8301
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1743601"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="VF097_08240"
     CDS             <1..1017
                     /locus_tag="VF097_08240"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6262818.1"
                     /translation="EAADRAAEATARLTRLLVRLSTHPEDTTDAVRVEVDEVAPVFEE
                     LGDERGLANVARLRAEIEWLASRFGAAAEHLERTIEHARRAGDRRLEMDALAQLAAAI
                     LFGPIPADEGIRRCREIYDRSGGDRRVEAAVLSVEAELRAMQGRFEGTREQISKAQAI
                     LEDLGLRFRSRASEALASVEMLAGDPEAARRAYMSGYETLEEIGERGFLSTTAAELAL
                     VAWAQEDFEDAERFVRISEESGASGDVATIVPAKRVRAKLLARQGSFEEAERIARQAV
                     DAARRSDDLNLQADALLDLAEVLRTAGREHEAIEPLEEAAALYEEKGNLVGAQRARAL
                     LRSP"
     gene            1079..1249
                     /locus_tag="VF097_08245"
     CDS             1079..1249
                     /locus_tag="VF097_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262819.1"
                     /translation="MPRHYRIMISIQDEDDNEIDSSVHEEDYEDDDEARRRFQEKAQA
                     ASGAGRGRPQRQ"
     gene            1263..1772
                     /locus_tag="VF097_08250"
     CDS             1263..1772
                     /locus_tag="VF097_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015659032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262820.1"
                     /translation="MATIAQERAGPAETRLRVGSWLMGLAAAGFIAYAVVFLILSFTS
                     RFLELGIGPEQVDVGRDEIRAFSPSLSHYIDHLHIAVAGFIAAIGAAVLFLVMFGVRR
                     GALWAWVGAVAATVLALGISLPAHYPNGFDTLAHLGPVYVATAIFAAGAALALRGILA
                     ERRARGNLP"
     gene            complement(1769..4090)
                     /locus_tag="VF097_08255"
     CDS             complement(1769..4090)
                     /locus_tag="VF097_08255"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX6262821.1"
                     /translation="MSTPVLKRVFVGRALASHTLEHQRLPKTLALPIFSSDPLSSNAY
                     ATEEMMLVLVLAGTAAFRLMIPIALLIVLVLSIVITSYRQTVRAYPGGGGSYIVAHEN
                     LGPLPGVIAASALLSDYVLTVAVSITAGTLAITSAAPGLLPYKVPLALGLIVLVAMAN
                     LRGVKEAGMLFAVPAYGFVIAVYVLLITGFLRCTNGCPTAETADLPIQPEHALTFFLI
                     LRAFASGSTALTGVEAISNGVQAFRRPQAKNAAATLAVMGAMTVGMFLGITVLARSIQ
                     LRVTEELAAEKSVLAQIGETVFGGDDPMFYVLQAFTALILILAANTAYQDFPRLSAIL
                     ATDHYMPRQFMNRGDRLVFSNGIVALSILAGLLVWIFDANLTRLIQLYLVGVFVAFTL
                     SQAGMVRRWLRLRERGWRRSALINGVGATATGVVLVIITGTKFTHGAWIVVLAIPLIV
                     LLLLSIYRHYERVRVTLRSRGLTMDAEVENTFVVLVPDLGLATREAISWLVTVRPDPG
                     RVIPLYVGPDPLEVAAAEWARLAPRLGELRRLEVHNGQEVRSLRRFVRGIERGPDDFV
                     TVVVPERVSTGLLLYVLRRTRSFRVKAAFLLEPGVVVTDVPLLPEELEVAAGRALEER
                     PLELARNVCIVPVSAVHDATMRALAYARSLRPAHLEAIFLAGEPQEADEILADWREKR
                     VDVPLSAIEAPFRDYGPPFLEEIRRHTRRGDTIVTVVLPEFVVDRWRHALLHNQRALF
                     FKRTLLVEPWVVAVSVPYQIGAARRAERAPSPA"
     gene            complement(4087..4272)
                     /locus_tag="VF097_08260"
     CDS             complement(4087..4272)
                     /locus_tag="VF097_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262822.1"
                     /translation="MVASLGEVGPFLGGLVVGVLIGVVLTPLVGFWVRRHEWKEASRK
                     ARLTEDVLDLMDSMAPR"
     gene            4428..5810
                     /locus_tag="VF097_08265"
     CDS             4428..5810
                     /locus_tag="VF097_08265"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6262823.1"
                     /translation="MLLPAMTALALTTVPTLLAALPARASTATAAMAYVLSVTVASAL
                     GGLRAGLATSVLSFLALNFFFTPPFLTLTVGRVEDVVALAVFLIVSGIVGTLLSRAVS
                     QRARAERREREARLLHRVGTRLLSGEEVGDILESFARAVTELFSLARCEITTELTPEP
                     VVVETATDGAGEPETVEMSARGRRVGRISMTPTDPAGLGAAEREVVQTLANQMGLAIE
                     GVRLGSEATTARVEAETNRLRAALFSSVTHDLRTPLASITASVTSLQDEDARFTEEDR
                     SELLETIRQEAERLDRLVADLLDLSRIRAGALIPTKEKASIEELIEGVVGRLHPLLAG
                     HEIALRFRPDLPEVPMDIVQIDQVLTNLLENAAKHSPPGTQITIAAARWQTMIEVRVI
                     DRGTGIPVEERERVFELFATGDGDSRGTGMGLAIGQAIVQSHGGRIWIEGTPGGGTTV
                     VFQLPVGEVG"
     gene            5807..6511
                     /locus_tag="VF097_08270"
     CDS             5807..6511
                     /locus_tag="VF097_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6262824.1"
                     /translation="MTIRVLVVDDDPQILRAVRTSLRARGFDVHTAPNGETALDALSE
                     EGTELVILDLGLPGLDGQEVLRRLRSWSDVPVIVLTVRDAQSEKVSALDAGADDYVVK
                     PFAMEELLARMRAVRRRVSAGASEAVLRFAELEVDLARQAVRVNGELVHLTPTEYRLL
                     ETLVTHPGKLLTHGWLLRRVWGPRYGDESHYLRVYVQQLRRKLRDDPGKPRFILTEPG
                     LGYRWMPEPQNDPDRR"
     gene            complement(6533..7195)
                     /gene="hemQ"
                     /locus_tag="VF097_08275"
     CDS             complement(6533..7195)
                     /gene="hemQ"
                     /locus_tag="VF097_08275"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HEX6262825.1"
                     /translation="MSEMVTYAYYPAFRRLPSIAEVAGDAAHEWEVLFKEHEDRVQVR
                     GFYAAEGFRPDADLLMWWVAESADAHQDLLIALRRTAIGRHLELTHAFMGAVRPPEFN
                     PDHQPAFMRGEAPRRYLCVYPFVRDAEWYLIPPEERAEMLREHGVMGREFPGILPNTT
                     SAFGLGDWEWILAFESDELHEIVDCIRRLRDAKARRHTKEEVPFVVGIRKELPEVLAD
                     LL"
     gene            complement(7192..8082)
                     /gene="hemH"
                     /locus_tag="VF097_08280"
     CDS             complement(7192..8082)
                     /gene="hemH"
                     /locus_tag="VF097_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX6262826.1"
                     /translation="MTIGVLVMAYGTAAGPDDIERYYTDIRGGRPPSPELLEELRVRY
                     AAIGNRFPLTEITEAQAAGVERELGEGLRAFVGMKHSPPFIPETVEKMAADGIDRAVG
                     IVMAPHYSRMSIGGYVERVEKAEAPIEVDVIESWHTHPAYLEVLADRVRRALDTLPED
                     PLVVFTAHSLPARIVGEGDPYPDQLRQTADAIAERLGLAHHTTGWQSAGRTPEPWLGP
                     PLEELIPEVAKEHAAILVVPCGFVSDHLEILYDLDVEARGIAEREGIAFARTESLNAE
                     PDFVAALATIVRDRLASWDR"
     gene            complement(8079..>8301)
                     /locus_tag="VF097_08285"
     CDS             complement(8079..>8301)
                     /locus_tag="VF097_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX6262827.1"
                     /translation="AWERVGPRAIQGNLDPTACIGSWEACEREAIAVLDRAGGRPGHI
                     FNLGHGVLPETPPDNLARLVDLVHERTAR"
BASE COUNT         1277 a         2876 c         2996 g         1152 t
ORIGIN      
        1 gaagccgccg accgcgcggc cgaagccacc gcccgcctca cccgcctcct ggtccgcctc
       61 tccacccatc ccgaggacac caccgacgcc gttcgcgtcg aggtggacga ggtggccccg
      121 gtgttcgagg agctggggga cgagcggggc ctggccaacg tggcccgtct ccgcgccgag
      181 atcgagtggc tggcctcgcg cttcggcgcg gccgcggagc acctggagcg gaccatcgag
      241 cacgcacgcc gggccggcga ccgccgcctg gagatggacg cgctcgctca gctcgccgcg
      301 gcgatcctgt tcggccccat tcccgccgac gaggggatcc ggcgctgccg cgagatctac
      361 gaccgctccg ggggcgaccg gcgcgtcgag gccgcggtcc tctcggtgga ggcggagctc
      421 cgcgccatgc agggccggtt cgaaggcacc cgggagcaga tctccaaggc ccaagccatc
      481 ctcgaggacc tcggcctccg cttccgctcc cgtgcctccg aggcgctggc ctccgtcgag
      541 atgctggccg gcgaccccga ggccgcccgc cgcgcctaca tgagcggcta cgagaccctc
      601 gaggagatcg gcgagcgcgg cttcctctcc acgaccgccg cggagctggc tctcgtggcc
      661 tgggcgcagg aggacttcga ggacgcggag cggttcgtgc ggatcagcga ggagagcggc
      721 gcctccgggg acgtggccac gatcgtgccc gccaagcggg tccgcgccaa gctgctcgcc
      781 cgccagggca gcttcgagga ggccgagcgc atcgctcgcc aggcggtgga cgccgcccgc
      841 cggtccgacg acctcaacct gcaggccgac gccctgctcg acctagccga ggtcctccgg
      901 acggccggcc gtgagcacga ggcgatcgag ccgctcgagg aggcggccgc cctctacgag
      961 gagaagggca acctcgtggg cgcccagcgg gcccgagccc tcctccgttc cccctgaccg
     1021 gttgcacgcc gaagcatcac cggaacacaa tggagcccga gagaccggga gggggcgtat
     1081 gccgaggcac taccggatca tgatctcgat ccaggacgag gacgacaacg agatcgactc
     1141 gtcggtccac gaggaggact acgaggacga cgacgaggct cgtcgcaggt tccaggagaa
     1201 ggctcaggcc gccagcgggg ccgggagagg ccgcccacag cgccagtagg ccggacacgg
     1261 ccatggccac gatcgcccag gaacgagcgg ggcccgcgga gacccggctc agggttggat
     1321 cctggctcat gggcctggcc gccgccggct tcatcgcgta cgccgtcgtc ttcttgatcc
     1381 tgagcttcac gagtcggttc ctagagctgg ggatcggtcc ggagcaggtc gacgtgggcc
     1441 gcgacgagat ccgggcgttc agcccctcgc tctcccacta catcgatcac ctgcacatcg
     1501 cggtggccgg cttcatcgcg gcgatcgggg ccgccgtgct gttcctcgtg atgttcgggg
     1561 tgcggcgagg agccctctgg gcctgggtcg gggcggtcgc cgcgacggtc ctcgcgctgg
     1621 ggatctcgct tcccgcccac taccccaatg gcttcgacac cctcgcgcac ctcgggcccg
     1681 tgtacgtggc aacggcgatc ttcgccgcgg gcgcggcgct cgcactccga gggatcctgg
     1741 cggagcgacg agcccggggg aacctcccct aggccggcga gggagctctc tcggcgcggc
     1801 gggccgcgcc gatctggtag gggacgctga cggcgaccac ccacggctcc acgagcagcg
     1861 tgcgcttgaa gaagagagcc cgctggttgt ggaggagagc gtggcgccac cgatcgacca
     1921 cgaactcggg caggaccacc gtcacgatgg tgtccccgcg cctcgtgtgc cggcggatct
     1981 cctccaggaa gggtggcccg tagtcgcgga acggcgcctc gatcgcgctc agcgggacgt
     2041 cgacccgctt ctccctccaa tcggccagga tctcgtcggc ctcctgcggc tccccggcca
     2101 ggaagatcgc ctccaggtga gccggccgga gcgagcgggc gtaggccagc gcccgcatcg
     2161 tcgcgtcgtg aaccgccgac accgggacga tgcacacgtt gcgagccagc tccagggggc
     2221 gctcctcgag cgctcggccg gcggcgacct ccagctcctc ggggaggagg ggcacgtccg
     2281 tgaccaccac gccgggctcc agcaggaagg cggccttcac gcggaaggac cgggtcctgc
     2341 gcaggacgta gaggagcagc cccgtcgaga ccctctcggg gacgaccacc gtgacgaagt
     2401 cgtcggggcc gcgctcgatc ccgcggacga accggcggag ggaccggacc tcctgaccgt
     2461 tgtggacctc caggcggcgg agctcgccca ggcgtggggc gagcctggcc cactcggccg
     2521 cggcgacctc caggggatcg gggcccacgt agagggggat gacccgaccg ggatccgggc
     2581 gcacggtaac cagccatgag atcgcctccc tggtggcgag ccccagatcg ggcaccagga
     2641 cgacgaacgt gttctccacc tcggcgtcca tcgtgaggcc cctggagcgc agcgtgacgc
     2701 gcacgcgctc gtagtgacgg tagatcgaga gcagcaggag cacgatgagc gggatcgcca
     2761 gcaccacgat ccacgctccg tgggtgaact tcgtccccgt gatgatcacc agcaccacgc
     2821 ccgtcgccgt ggcaccgacg ccgttgatca gggcgctccg gcgccacccc ctctcccgca
     2881 gcctgagcca ccggcgcacc atgcccgcct gggacagggt gaaggccacg aagacaccga
     2941 ccaggtagag ctggatcagc ctggtgaggt tggcgtcgaa gatccagacg aggagccccg
     3001 ccaggatcga gagcgcgacg atgccgttcg agaacacgag ccggtcgcct cggttcatga
     3061 actgccgcgg catgtagtgg tcggtggcga ggatcgccga gagtcggggg aagtcctggt
     3121 acgccgtgtt tgccgcgagg atgaggatca gcgccgtgaa cgcctgcagc acgtagaaca
     3181 tcgggtcgtc cccaccgaag accgtctccc cgatctgcgc cagcaccgac ttctccgcgg
     3241 ccagctcctc ggtcacgcgc agctggatcg acctcgccag caccgtgatg ccgaggaaca
     3301 tcccgacggt catcgctccc atgacggcga gcgtcgcggc cgcgttcttc gcctgcgggc
     3361 ggcggaacgc ctggacgccg ttcgagatgg cctccacgcc ggtgagggcc gtggagcccg
     3421 aggcgaaggc tcggaggatg aggaagaacg tgagcgcgtg ctcgggctgg atcgggaggt
     3481 cggcggtctc cgcggtcgga catccattcg tgcaccggag gaagcccgtg atcagcagca
     3541 cgtagaccgc gatcacgaac ccgtacgcgg ggacggcgaa gagcatcccc gcctccttca
     3601 cgccccggag gttggccatc gccacgagca cgatgagccc cagggccagc ggcaccttgt
     3661 agggcagcag ccccggcgcc gcggacgtga tcgccagggt ccccgccgtg atgctgacgg
     3721 ccaccgtcag gacgtagtcg gacaggagcg cggaggcggc gatcacgccc gggagcggcc
     3781 cgaggttctc gtgggccacg atgtacgagc ccccgccgcc cgggtacgct cgcaccgtct
     3841 gccggtacga cgtgatcacg atcgacagaa ccagcacgat gagcagagcg atcgggatca
     3901 tgaggcggaa cgccgcggtc ccggccagca ccaggacgag catcatctcc tcggtcgcgt
     3961 aggcgttcga ggagaggggg tccgaggaga agatgggaag ggcgagggtc ttcggcaggc
     4021 gctgatgctc cagcgtgtgg ctcgccagcg ctcgtccaac gaacacgcgc ttcaggaccg
     4081 gggtgctcat cggggcgcca tcgagtccat cagatcgagc acgtcctcgg tgagacgcgc
     4141 cttccgggag gcttccttcc attcgtgccg cctcacccag aagccgacca gaggggtcag
     4201 gacgacgccg atcagcaccc cgaccaccag tccgccgagg aacggtccaa cttcgccgag
     4261 cgatgccacc atggtccggc gatggtgacg ccggagccgt caggaccccg tcaggacgag
     4321 ccgtcgaggc gtaaagaagg cgtatggaac tacccggccg gttgtggggc cctccgggca
     4381 gggcgagagt gtttgggatg aacggtcgcg gagaccggag aggcagggtg ctcctgcccg
     4441 ccatgacggc gctcgcgctc acgaccgtcc cgaccctgct cgctgcgctc cccgccaggg
     4501 cgtccaccgc caccgccgcg atggcctacg tcctctcggt gaccgtggcg tcggcgctgg
     4561 ggggcctgcg cgcggggctc gcgacgtccg tgctctcgtt cctcgccctg aacttcttct
     4621 tcaccccacc cttcctcacc ctgacggtgg ggagggtcga ggacgtggtc gccctcgccg
     4681 tcttcctgat cgtctccggg atcgtcggga cccttctctc ccgagccgtc tcgcagaggg
     4741 cacgagcgga gcgtcgcgag cgtgaggccc gtctcctcca tcgcgtcggc acccgcctgc
     4801 tttccggcga ggaggtcgga gacattctgg agagcttcgc tcgagccgtc accgagctct
     4861 tctccctcgc tcgatgcgag atcaccacgg agctgacgcc cgagccggtg gtggtggaga
     4921 ccgcgacgga cggagccggg gagccggaga ccgtcgagat gagcgcccgg ggacggcgcg
     4981 tgggccggat ctcgatgacc ccgaccgacc cggccggcct gggagctgcc gagcgggagg
     5041 tggtccagac cctcgccaac cagatgggcc tggccatcga gggtgtgcgg ctcggcagcg
     5101 aggccacgac cgctcgcgtc gaggccgaga cgaaccggct ccgggccgcc ctcttctcct
     5161 ccgtcaccca cgacctgagg accccgctgg cctcgatcac ggcgtccgtc acgagcctcc
     5221 aggacgagga cgcgcgcttc accgaggagg atcggagcga gctgctggag acgatacggc
     5281 aggaagccga gcgcctcgac cgcctggtgg cagacctcct cgatctctcg cggatccggg
     5341 cgggggccct gatcccgacc aaggagaagg cctcgatcga ggagctgatc gaaggcgtgg
     5401 tgggccggct gcatccgctg ctggcaggcc acgagatcgc cctccgcttc cgcccggatc
     5461 ttcccgaggt gccgatggac atcgtccaga tcgaccaggt gctgacgaac ctgctggaga
     5521 acgcggcgaa gcacagcccg ccgggaacgc agatcacgat cgcggcggcc cgatggcaga
     5581 cgatgatcga ggtgcgggtg atcgatcgag ggacgggcat ccccgtcgag gagcgcgaac
     5641 gggtcttcga gctgttcgcc accggcgacg gcgactcgag ggggacgggg atgggcctcg
     5701 cgatcgggca ggccatcgtc cagtcgcacg gagggaggat ctggatcgag ggcacccccg
     5761 gaggcgggac gaccgtcgtc ttccagcttc cggtcggaga ggtcggatga cgatccgggt
     5821 gctggtcgtg gacgacgatc cccagatcct ccgtgccgtg cggacgagcc tccgggcgcg
     5881 cgggttcgac gtgcacaccg ctcccaacgg cgagacggcg ctggacgcgc tctccgagga
     5941 gggcaccgag ctggtgatcc tcgacctggg actccccggc ctggacggcc aggaggtgct
     6001 tcgtcggctc cgctcctggt cggacgtgcc cgtgatcgtg ctgaccgtcc gggacgcgca
     6061 gagcgagaag gtatcggccc tcgacgccgg ggccgacgac tacgtcgtca agccgttcgc
     6121 gatggaggag ctgctggcga ggatgcgggc cgtgcggcgg cgcgtctcgg cgggcgcgtc
     6181 ggaggcggtc ctgcggttcg cggagctcga ggtcgacctg gcacgtcagg ccgtgagggt
     6241 gaacggagag ctcgtgcacc tcacccccac cgagtaccgc ctcctcgaga cgctggtgac
     6301 gcatccgggg aagctgctca ctcacgggtg gctgctccgg cgggtgtggg gaccgaggta
     6361 cggggacgag tcccactacc tcagggttta cgtccagcag ctccggagga agctccggga
     6421 cgatcccggg aagccacggt tcatcctcac ggagccgggg ctgggctacc gctggatgcc
     6481 cgagccccag aacgacccgg atcgtcgttg actgtcgcaa cctgctccgc ggctagagca
     6541 ggtcggccag gacctccggg agctccttgc ggatgccgac gacgaagggg acctcctcct
     6601 tcgtgtggcg gcgggccttc gcgtcgcgga gacgacggat gcagtccacg atctcgtgga
     6661 gctcgtccga ctcgaaggcg aggatccact cccaatcccc cagcccgaac gcgctggtcg
     6721 tgttgggaag gatgcccggg aactcccggc ccatcacccc gtgctcgcgc agcatctcgg
     6781 cccgctcctc gggcgggatc aggtaccact ccgcgtcgcg gacgaagggg tagacgcaga
     6841 ggtagcgtcg cggcgcctcc ccccgcatga acgcgggctg gtggtcgggg ttgaactcgg
     6901 ggggccggac ggcgcccatg aaggcgtggg tcagctcgag gtgacggccg atcgccgtgc
     6961 ggcgcagcgc gatcagcaag tcctggtggg cgtcggccga ttccgccacc caccacatca
     7021 ggaggtcggc gtcgggacgg aagccctcgg ccgcgtagaa cccgcgcacc tgcacacggt
     7081 cctcgtgctc cttgaagagc acctcccact cgtgggccgc gtcaccggcg acctcggcga
     7141 tcgacggcag ccggcggaac gccgggtagt aggcgtaggt gaccatctcg ctcatcgatc
     7201 ccatgatgcc agacgatctc ggacgatggt cgcgagcgcg gcgacgaagt ccggctcggc
     7261 gttgagcgac tcggtgcggg cgaaggcgat gccctcgcgc tctgcgatcc cgcgggcctc
     7321 gacgtcgaga tcgtagagga tctcgaggtg gtccgagacg aacccgcagg ggacgacgag
     7381 gatcgcggcg tgctccttgg cgacctcggg gatcagctcc tcgagcggtg ggccgagcca
     7441 gggctcgggg gtgcggccgg cgctctgcca gccggtggtg tggtgggcga ggccgaggcg
     7501 ttccgcgatc gcatcggcgg tctggcggag ctggtccggg tacgggtcgc cctcgcccac
     7561 gatccgcgcg gggaggctgt gggcggtgaa gacgacgagc gggtcctcgg ggagggtgtc
     7621 gagggcgcgg cgcacgcggt cggccagcac ctcgaggtac gcggggtgcg tgtgccagga
     7681 ctcgatcacg tccacctcga tgggggcctc cgccttctcg acgcgctcca cgtagccccc
     7741 gatcgacatc ctcgagtagt gcggcgccat cacgatcccc accgcgcggt cgatcccatc
     7801 ggccgccatc ttctccaccg tctcggggat gaagggcggc gagtgcttca tcccgacgaa
     7861 ggcccggagg ccctcgccca gctcgcgctc cactcccgcg gcctgggcct cggtgatctc
     7921 ggtcagcggg aagcggttgc cgatcgccgc gtagcgcacg cgcagctcct cgagcaactc
     7981 ggggctgggc gggcggcccc cgcggatgtc cgtgtagtag cgctcgatgt cgtccggccc
     8041 ggcggcggtg ccgtaggcca tcacgaggac gccgatggtc atcgggccgt cctctcgtgg
     8101 acgaggtcga cgaggcgggc caggttgtcc ggcggggtct cggggagcac gccgtggccg
     8161 aggttgaaga tgtgcccggg gcggccgccg gcgcggtcca gcacggcgat cgcctcgcgc
     8221 tcgcaggcct cccaggaccc gatgcacgcg gtgggatcga ggttgccctg gatcgccctg
     8281 ggcccgaccc gctcccaggc t
//
LOCUS       DASUTZ010000186         6994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_837429, whole genome shotgun sequence.
ACCESSION   DASUTZ010000186 DASUTZ010000000
VERSION     DASUTZ010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6994
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837429"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="VF097_08290"
     CDS             complement(<1..1158)
                     /locus_tag="VF097_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein FdrA"
                     /protein_id="HEX6262828.1"
                     /translation="MSATAVRVRPDTYVDSVVLMSGARAMRDLDGVAWATAVMGTDAN
                     LDELRSRGFKGDDLGSARANDLVLAVEAGSEEEAREALDAGMRALSESGGGAAEGPKE
                     RGPRSLQQAVADAPDVNVALISVPGEHAPLEAHKALSAGLHVLLFSDNVPLDDEVELK
                     VRGRELRRFVMGPGAGTAILGGVGLGFANVVQRGPVGVVAAAGTGAQEVSSLLDRWGV
                     GVRDLIGVGGRDLSERVEGGMTRMALEAFEADDEVEVVLLVSKPPAPDVAADLVRRLG
                     EGKPAVAVLLGLADDLDPPEGVLLARTLEDGVLRTLEVLGRDGPELSRGLEERVGEAI
                     EGLDRDRTAVRGLFSGGTLCYEAMIVLSERLEAEVRSNTPLKEGWGLPAPDG"
     gene            complement(1155..2108)
                     /gene="arcC"
                     /locus_tag="VF097_08295"
     CDS             complement(1155..2108)
                     /gene="arcC"
                     /locus_tag="VF097_08295"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011251108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEX6262829.1"
                     /translation="MRSVVVALGGNAITREGEAGAYEEQEANARRMARAIARMVGAGH
                     RVVVTHGNGPQVGNLAIQQEEGASLVPAQPLFALGAMTQGQIGHLLGLALAPLVETKV
                     ASVVTHVVVDPRDPAFADPTKPIGPFFHEATARRLASARGWTVREDAGRGYRRVVPSP
                     EPKEILESGVIRALLRQGAVVIASGGGGIPVVRRGKRLVGVDAVIDKDLSAERLGTEI
                     GADDLLMLTGVDRVALGFGTREERPIREMTADEAETYMSEGHFPPGSMGPKIAAAVRF
                     VREGGEAAIVTSPRRVGAALAGNHGTRIVPAREAPRRAAGQ"
     gene            complement(2108..2665)
                     /locus_tag="VF097_08300"
     CDS             complement(2108..2665)
                     /locus_tag="VF097_08300"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6262830.1"
                     /translation="MEEVIAAIGPKVRQLRRQKNLSLQQLAERSGVSAAAIHKLERNG
                     MVPTITTLMKLASALNRPVSYFVDEQDPDHAVVVRSGDRKTVYTSKSGLGLESVSGPY
                     GRFFMAGAVATVEPKADSGPKPMEHPGEELVYVLSGSLRFEIDGEEHVLRRGDALHFR
                     TDRPHRWWNPGARPAKALWMALRST"
     gene            2783..4174
                     /locus_tag="VF097_08305"
     CDS             2783..4174
                     /locus_tag="VF097_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6262831.1"
                     /translation="MAQRYVVVGGGAAGIGAAGAIKGVDREADLQVFTEYEDAAYSPC
                     GIPYVHGREIDSFERLFLQGKEYYKEQGIDIRYETRVGRIDLSAREIELAPLGQTPFD
                     TERRTDEPAGGGERIPFDKLIVATGFDYEAPDIPGTDLEGQYYVKNIRRAMEWDKVLD
                     DAKSVVVLGASPLGLEMITSMAHRGLETHVVDPHPWALAEAADPDIVEPVQESWTEMG
                     VTMHFNTKAEALVGEGRVRGVKTSEGEIPCDLVIICTHKRPNNRLAAEAGLKIGSTGG
                     IVVDEGMRASVDGVFAAGDVAEIPHGLTKLPLQGLSGSHAYAQGKVAGTNAAGGDRAY
                     RPVYVPWGMVAGKWMIGGVSFGESTATALGIPFVMGVAQGISRARYYPDSKMIKVKLL
                     AEPGTLRLIGAQMVGGEGIKERADFLAMCARKGITLGDLATMENVYSPPIGALGEPIS
                     AAAQNALTEQGLW"
     gene            4174..4434
                     /locus_tag="VF097_08310"
     CDS             4174..4434
                     /locus_tag="VF097_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262832.1"
                     /translation="MGFSKWLRSNAESFLLEATEHDLAKKYGLRPPVRRRGVMAFLWR
                     RVFVPVYRILPWSLRRWIMGIMPGSHRQTWQDRAPPYRKTAA"
     gene            4478..4963
                     /locus_tag="VF097_08315"
     CDS             4478..4963
                     /locus_tag="VF097_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0572/Sll0783 family nitrogen starvation
                     response protein"
                     /protein_id="HEX6262833.1"
                     /translation="MADFVNEPVEAGQAIVDYEGKVFEDIQAEPGQKAFVFMHTVPFE
                     GSVGIVNMLTATRLVRKGFELHFVLFGPGSLMASKTRGFPKVGDEAFPGHLNFNNQLQ
                     TIMDEGGKVYACRFSAAALYGMRESDMMDGVIPIHPRDVADAALTAWREGAFTMNTWN
                     L"
     gene            5031..5648
                     /locus_tag="VF097_08320"
     CDS             5031..5648
                     /locus_tag="VF097_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262834.1"
                     /translation="MRPIERLAAEWSAAEERITSLVMTRPEAYERHLRAARLVADALS
                     EVGSREELVATFEEGEALAERVLHETGYPPDEVSASQVAGAGFAIRYRELVRELARAE
                     AADRIREARRRGDAWVVVEEQGHADAPMPGLYRRLEMRLADGAGLHAFVDEDPGTGAP
                     RFGAERVPLDPETGDATGDPSDRRMFGQREEWERHVRAIREGGPS"
     gene            5716..6582
                     /locus_tag="VF097_08325"
     CDS             5716..6582
                     /locus_tag="VF097_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006917634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6262835.1"
                     /translation="MADGEKVSGRVIDGHNHIGEMEAWAFYDLEEPVKPTVFEFKDGK
                     DYIKNHLDRYGIERAMVIPNYGIPKQDQPFGLNAVALEAAQSSDRLSCAIWTSFMGQN
                     KELTMEALKLAGEDGVKALKTTFLLGGSPNPADWDDDDREVAEACLRAAEEHDLVFHF
                     HTSPGGSSDISNYIPLIEEWGKRIKIYLVHFGGGVSGHIKLVPKFLDWVEQGLQVYTD
                     CTWSVGFGPRWLMTEIEKRGIGEDRVLFSSDVPWSDFWGEYYKMTGQPGISQELKDRI
                     LHRNFEELYGND"
     gene            6569..>6994
                     /locus_tag="VF097_08330"
     CDS             6569..>6994
                     /locus_tag="VF097_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX6262836.1"
                     /translation="MATTDLDLRRLIVELQSHGVRVETSDVEGRKGGAGPTDSGFVYL
                     DGIPVTFPFTGDYLTDSPFTMRREGDAWVLYRDEERLAEADLPPRPKIYDLETEDGIP
                     YWQIALLHLDSLASTVVQTCVHWGTPDQCTFCGIEVSLEA"
BASE COUNT         1053 a         2453 c         2524 g          964 t
ORIGIN      
        1 gccgtcgggg gcggggagcc cccagccctc cttgagcggc gtgttcgatc ggacctccgc
       61 ctcgaggcgc tcgctcagca cgatcatcgc ctcgtagcag agggtgcccc ccgagaacag
      121 gcctcgcacg gcggtccgat cccggtccag gccttcgatc gcctcgccga cccgctcctc
      181 cagccctcgg gagagctcgg gcccatcccg gcccaggacc tccagcgtgc gcagtacccc
      241 gtcctccagg gtgcgggcca gcaggacccc ttccggtggg tcgagatcgt cggccaggcc
      301 caagaggacc gccaccgccg gcttgccctc gcccagccgc cgcacgaggt ccgccgccac
      361 gtcgggcgcc ggcggcttgg agacgagcag gaccacctcc acctcgtcgt cggcctcgaa
      421 ggcctccagc gccatgcggg tcatcccccc ctccacccgc tcggagaggt cgcggccccc
      481 cacgccgatc aggtcgcgca cgccgacccc ccagcggtcg aggagcgagc tgacctcctg
      541 ggctcccgtc ccggccgccg ccaccacgcc gacaggtccc cgctggacca cgttggcgaa
      601 cccgagcccc acgccgccga ggatcgcggt ccccgcgccc ggccccatca cgaagcgccg
      661 gagctcacgc ccgcggacct tcagctcgac ctcgtcgtcg agggggacat tgtcgctgaa
      721 cagtaggacg tggaggccgg cggacagcgc cttgtgggcc tcgagcggag cgtgctcgcc
      781 ggggaccgag atgagcgcga cgttgacgtc cggcgcgtcc gccaccgcct gctgcagcga
      841 gcgggggccg cgctccttcg ggccctcggc ggccccgccc ccggactccg acagcgcccg
      901 catgccggcg tcgagcgcct cccgcgcctc ctcctcggac ccggcctcca cggccagcac
      961 gaggtcgttg gcccgggccg agcccaggtc gtcgcccttg aacccccggg accggagctc
     1021 gtcgaggttg gcgtcggtgc ccatcaccgc cgtggcccag gccacgccgt ccaggtcccg
     1081 catggcgcgg gctcccgaca tcaggacgac cgagtcgaca taggtgtcgg ggcggacgcg
     1141 gacggcggtc gcgctcactg cccggccgcc cgccgcggcg cctccctcgc ggggacgatc
     1201 cgggtcccgt ggttgccggc gagcgccgcg ccgacccgcc tcggggacgt gacgatcgcc
     1261 gcctcgcccc cctctcgcac gaacctgacg gcggcggcga tcttcggtcc catgctcccg
     1321 ggggggaagt gcccctcgga catgtaggtc tcggcctcgt cggccgtcat ctcccggatc
     1381 gggcgctcct cgcgcgtgcc gaagccgagc gccacgcggt ccacgcccgt gagcatcagc
     1441 aggtcgtcgg cgccgatctc ggtcccgaga cgctcggcgg agagatcctt gtcgatcacg
     1501 gcgtccaccc cgaccagcct cttccccctc cgcacgacgg ggatgcctcc gccccccgag
     1561 gcgatgacga cggccccctg ccgcagcagg gcccggatca cgccggactc caggatctct
     1621 ttcggctcgg gggaggggac cacccggcgg taccctcggc cggcgtcctc gcggacggtc
     1681 cacccgcgcg ccgaagcgag cctgcgggcg gtggcctcgt ggaagaacgg cccgatcggc
     1741 ttcgtcgggt ccgcgaacgc ggggtcgcgt gggtccacca ccacgtgggt aacgacggag
     1801 gccaccttcg tctccacgag gggggccagg gccagcccca ggaggtggcc gatctggccc
     1861 tgcgtcatcg cgccgagcgc gaacagcggc tgggccggca cgagggaggc cccctcctcc
     1921 tgctggatcg cgaggttgcc gacctggggg ccgttgccgt gggtaacgac gacccggtgg
     1981 ccggccccga ccatccgcgc gatggcccgg gccatgcgcc gagcgttcgc ctcctgctcc
     2041 tcgtacgcgc cggcctcgcc ctcgcgggtg atggcgttcc ctccgagcgc gacgacgacc
     2101 gagcgcatca cgtcgaccgg agggccatcc agagggcctt cgccgggcgg gccccggggt
     2161 tccaccaccg gtgcggacgg tcggtgcgga agtgcagcgc gtcgccgcgc cgcaggacgt
     2221 gctcctcgcc gtcgatctcg aagcggagcg agccgctcag gacgtacacg agctcctccc
     2281 ccggatgctc catcggtttg ggcccgctgt cggccttggg ctccaccgtg gccaccgcgc
     2341 ccgccatgaa gaaccggccg tagggcccgg agacgctctc caggccgagc ccggacttcg
     2401 aggtgtacac ggtcttccgg tcgcccgacc gcacgacgac ggcgtggtcg ggatcctgct
     2461 cgtccacgaa gtaggacacg ggccggttga gggccgaggc cagcttcatg agcgtcgtga
     2521 tcgtggggac catcccgttc cgctccagct tgtggatggc cgccgccgag acccccgacc
     2581 gctcggcgag ctgctggagc gagaggttct tctgccggcg cagctgtcgg accttcggcc
     2641 cgatcgccgc gatgacctcc tccatgacgc gccctccact tgaagattcg cgatgacccc
     2701 gggtagagtt cgggtatcgg tgaacggcgc gttgagtata gtgaacaact cgctcgacgg
     2761 gcaatcctga ggagaggcgt acatggctca gcgctacgtg gtcgtgggcg gcggcgccgc
     2821 gggcatcggc gcggccgggg cgatcaaagg cgtcgatcgc gaggccgatc tccaggtgtt
     2881 caccgagtac gaggacgccg cctacagccc ctgcgggatc ccctacgtcc acggccgcga
     2941 gatcgactcg ttcgagcgcc tgttcctcca ggggaaggag tactacaagg agcagggcat
     3001 cgacatccgc tatgagacac gcgtgggtcg gatcgacctg agcgcccgag agatcgagct
     3061 ggccccgctg ggccagaccc cattcgacac cgagcgccgc acggacgagc cggcgggcgg
     3121 cggcgagcgc atccccttcg acaagctgat cgtggccacg ggattcgact acgaggcgcc
     3181 ggatatcccc gggaccgacc tcgaaggcca gtactacgtg aagaacatcc gccgggcgat
     3241 ggagtgggac aaggtcctcg acgacgcgaa gtcggtggtg gttctgggcg cctcccccct
     3301 gggcctggag atgatcactt cgatggccca ccgcggcctg gagacgcacg tcgtggaccc
     3361 ccacccctgg gccctggcgg aggccgccga ccccgacatc gtcgagcccg tccaggagtc
     3421 ctggaccgag atgggcgtga cgatgcactt caacacgaag gccgaggcgc tcgtggggga
     3481 gggtcgggtg cgcggggtga agacctcgga gggtgagatc ccctgcgacc tcgtgatcat
     3541 ctgtacgcac aagcggccca acaaccggct cgccgcggag gcgggcctga agatcggctc
     3601 caccgggggc atcgtcgtgg acgaggggat gcgcgcctcg gtggacggcg tgttcgccgc
     3661 cggcgacgtg gccgagatcc cgcacggcct gacgaagctc cccctccagg gcctgtcggg
     3721 gagccacgcc tacgcgcagg gcaaggtggc cggcacgaac gcggccggtg gggaccgggc
     3781 ctaccggccc gtctacgtcc cctggggcat ggtggccggc aagtggatga tcggcggcgt
     3841 ctcgttcggg gagagcacgg ccaccgcgct gggcatcccg ttcgtgatgg gcgtggccca
     3901 ggggatctcg cgggcccgct actacccgga cagcaagatg atcaaggtga agctgctggc
     3961 cgagccgggg acgctgaggc tgatcggggc tcagatggtt ggcggcgagg gcatcaagga
     4021 gcgggccgac ttcctggcga tgtgcgcccg taaggggatc acgctgggcg atctggccac
     4081 gatggagaac gtctactccc cgccgatcgg cgctctgggg gagccgatct cggcggcggc
     4141 gcagaacgcc ctcaccgagc aggggctctg gtgatgggct tctcgaaatg gctccggagc
     4201 aacgcggagt ccttcctcct cgaggccacc gagcacgacc tggcgaagaa gtacgggctg
     4261 cgcccgcccg tgcggcgccg gggggtcatg gcgttcctgt ggcgcagggt cttcgtgccc
     4321 gtctaccgga tcctcccctg gagcctgcgc cgctggatca tggggatcat gccggggagc
     4381 caccggcaga cgtggcagga ccgcgcgccg ccctaccgga agacggccgc gtgaagctgc
     4441 gggacgggca ccatcgtcga acggaaggag ggtcggagtg gcggacttcg tgaacgagcc
     4501 ggtggaggcc ggccaggcga tcgtcgacta cgaggggaag gtcttcgagg acatccaggc
     4561 ggagcctggg cagaaggcct tcgtgttcat gcacacggtg ccgttcgagg gctccgtggg
     4621 gatcgtgaac atgctcacgg cgactcgact ggtgcggaag gggttcgagc tgcacttcgt
     4681 gctgttcggg cccgggtcgc tgatggcgtc gaagacgcgc gggttcccca aggtgggcga
     4741 cgaggccttc ccggggcacc tgaacttcaa caaccagctc cagacgatca tggacgaggg
     4801 cggcaaggtc tacgcctgtc gcttctcggc cgcggccctg tacgggatgc gcgagtccga
     4861 catgatggac ggcgtgatcc cgatccaccc gagggacgtg gcggacgccg ctctcacggc
     4921 gtggcgtgag ggcgcgttca ccatgaacac ctggaacctc tgaggaggcg atcggcggag
     4981 gccccgggcc ggacgcgtcc ggcccggggc ctccgcgctg cctgggaccg atgagaccga
     5041 tcgagcggct ggcggccgag tggagcgccg cggaggagcg gatcacctcg ctcgtcatga
     5101 cccgccccga ggcctacgag cggcacctgc gggcggcccg gctcgtggcc gacgcgctgt
     5161 cggaggtcgg ctcccgggag gagctggtgg cgaccttcga ggagggcgag gcgctggcgg
     5221 agcgggtcct ccacgagacg gggtacccgc cggacgaggt gtccgcgtct caggtggcgg
     5281 gggcggggtt cgccatcagg taccgggagc tggtccggga gctggcccgg gccgaggccg
     5341 ccgatcggat ccgggaggcc cgccggcgcg gggacgcctg ggtggtggtg gaggagcagg
     5401 ggcacgccga cgccccgatg cccggcctct accggcgcct ggagatgcgg ctcgccgacg
     5461 gggccggcct ccacgcgttc gtggacgagg accccgggac cggggcgccc cgcttcgggg
     5521 cggagcgcgt cccgctcgac cccgagaccg gcgacgccac cggcgacccc tccgaccggc
     5581 gcatgttcgg ccagcgcgag gagtgggaac ggcatgtccg ggccatccgc gagggcggcc
     5641 cgtcttgaca tcgggctccg ggcggcgtga ggattccggt agaggctcgt ttcctatagt
     5701 gtcggagggg tagccatggc ggacggcgag aaggtcagcg gccgcgtcat cgacggccac
     5761 aaccacatcg gcgagatgga ggcctgggcg ttctacgacc tcgaggagcc ggtgaagccc
     5821 accgtcttcg agttcaagga cgggaaggac tacatcaaga accacctcga ccgctacggc
     5881 atcgagcgcg ccatggtgat ccccaactac gggatcccca agcaggacca gcccttcggc
     5941 ctcaacgcgg tggcgctgga ggccgcccag tccagcgacc gactcagctg cgcgatctgg
     6001 acctcgttca tgggccagaa caaggagctg acgatggagg cgctgaagct cgcgggcgag
     6061 gacggggtga aggcgctgaa gaccacgttc ctgctgggcg ggagccccaa cccggcggac
     6121 tgggacgacg acgaccgcga ggtggccgag gcgtgcctgc gcgcggccga ggagcacgac
     6181 ctcgtcttcc acttccacac gagccccggc ggctcatcgg acatcagcaa ctacatcccg
     6241 ctgatcgagg agtgggggaa gcgcatcaag atctacctcg tgcacttcgg gggcggggtc
     6301 agcggccaca tcaagctcgt cccgaagttc ctggactggg tggagcaggg cctgcaggtc
     6361 tacaccgact gcacctggtc ggtggggttc gggccgcgct ggctgatgac cgagatcgag
     6421 aagcggggta tcggcgagga ccgcgtgctg ttctcctccg acgtgccctg gagcgacttc
     6481 tggggcgagt actacaagat gacgggccag ccggggatca gccaggagct gaaggaccgg
     6541 atcctccacc ggaacttcga ggagctctat ggcaacgact gacctcgacc tgcgccgcct
     6601 catcgtcgag ctgcagagcc acggcgtccg cgtcgagacc tcggacgtcg aggggcggaa
     6661 gggcggggcg gggccgaccg actcggggtt cgtctacctc gacggcatcc cggtcacctt
     6721 ccccttcacc ggcgactacc tcaccgactc gccattcacg atgcggcggg aaggagacgc
     6781 gtgggtgctg taccgggacg aggagcggct ggcggaggcc gacctgcccc ccaggcccaa
     6841 gatctacgac ctcgagaccg aggacggcat cccgtactgg cagatcgcgc tgctgcacct
     6901 cgactcgctg gccagcaccg tggtgcagac gtgcgtccac tggggcacgc ccgaccagtg
     6961 cacgttctgc gggatcgagg tctcgctcga agcc
//
LOCUS       DASUTZ010000187         2663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_448521, whole genome shotgun sequence.
ACCESSION   DASUTZ010000187 DASUTZ010000000
VERSION     DASUTZ010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448521"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..996)
                     /locus_tag="VF097_08335"
     CDS             complement(28..996)
                     /locus_tag="VF097_08335"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX6262837.1"
                     /translation="MTRVGGLVLIAGGLLGTVVTVIAEGSRLRIPIALGLSGATLLLA
                     LGYLAAGERVPEHVLHAGPPIGSLLVASMIYVGGGGVTSAALAMPYVWIAMYAGYFHS
                     ARYMAGVVALGAAGHAVALLALGGPRSWPPIWLYAAATSAVVGAMVHYLVVRLRDLAA
                     TDALTGLPNRREWDELLSSHLRFAGRLGYRICVAMLDVDDLKGFNDSRGHAAGDEALR
                     SIARAGRTVLRGTDVLARVGGDEFGLLLPTCDPEGASEIMGRLRHAIAADVTVSVGVA
                     VWDGDEGADALMLRADVALYRAKRMGRDRVELAEVRPGREARALST"
     gene            complement(1104..1535)
                     /gene="dut"
                     /locus_tag="VF097_08340"
     CDS             complement(1104..1535)
                     /gene="dut"
                     /locus_tag="VF097_08340"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017535347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX6262838.1"
                     /translation="MRLEFQRLDPDVPLPSAAHEGDAGLDLRAWADLVVEPGRRALVP
                     TGLAVAIPEGHAGLVLPRSGLASRSGLTLANAPGLIDAGYRGEIKIAVVNLDPDEPVK
                     ISRGDRIAQLVVVPFSSVEPVAVDELPRTSRGVGGFGSTGS"
     gene            1620..2582
                     /locus_tag="VF097_08345"
     CDS             1620..2582
                     /locus_tag="VF097_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262839.1"
                     /translation="MSANLCSRCGRPLRDGVCPVGHPQRVARRRRRRPWRATLAIVIV
                     IALLAGAAYAALIWYPPRAAGDAIRPLSTELARTVPLFRQTVEALPSDSTPEALSEAS
                     AVLTAADEARSELTRLQGRLETTSVPSIPVISDRPPMETASRVHAQMSDFAVGALETV
                     GDLEASARYLTELAAILPSLANLRAAVGSPVAPGEVEGSVAASRPIAEQLRADLRSLT
                     PPEELSETHATLLAIARGLGTSIDELEATAGAASGPVIRAIVADIRSQISSFRETAGA
                     APQDARTGGLSDQIEDAERRLERIVAGLRELEDQGVEDLTIPDA"
BASE COUNT          408 a         1020 c          904 g          331 t
ORIGIN      
        1 acgacgagtc taccaatccg gctccgctca ggtcgagagc gcgcgggcct cgcgaccggg
       61 ccggacctcc gccagctcga cgcgatcgcg ccccatgcgc ttcgctcgat acagcgcgac
      121 gtcggcccgc agcatcaagg catcggcccc ctcgtcgcca tcccacacgg cgacacccac
      181 cgagaccgtt acgtcggctg cgatcgcgtg ccgcaagcgc cccatgatct cgctggcgcc
      241 ctccgggtcg caggtgggca ggagcaggcc gaactcgtcg ccacccacgc gcgccaggac
      301 gtcggtgccc cggagcacgg tccgccccgc acgggcgatc gaccggagcg cctcgtcgcc
      361 cgcggcgtgt cccctcgagt cgttgaatcc cttgaggtcg tccacgtcca gcatcgcgac
      421 acagatccgg tacccgaggc gccccgcgaa cctcaggtgg ctcgaaagga gctcgtccca
      481 ctcccgacgg ttgggcagac ccgtgagcgc atcggtggcg gccaggtcgc gaaggcgcac
      541 aacgaggtag tggaccatcg caccgaccac cgcggacgtt gccgccgcgt acagccagat
      601 cggcggccac gagcgtggcc cacccagcgc gaggagggcg accgcgtgcc ccgccgcgcc
      661 gagcgccacc acgcccgcca tgtaccgagc cgagtggaag tagcccgcgt acatcgcgat
      721 ccacacgtag ggcatcgcca gggcggccga ggtcaccccg ccgcctccca cgtagatcat
      781 cgacgcgacc agcaggctcc cgatcggggg gcccgcatgc aggacgtgct cgggaacccg
      841 ctcgccagcg gccaggtagc ccagcgccag gagcagcgtg gcgcccgaga gcccgagggc
      901 gatcgggatc cggagccgag agccctccgc gatcaccgtg accacggtcc ccaggagccc
      961 gccggcgatc aggaccaggc cacccacgcg ggtcatgtgc gcacgagtcg ctgctgggat
     1021 cggttccatc actaagtaca tcgacgcatc cgaagccggt catcacggcc cgccgtgacc
     1081 atcttgggca tagcccgttc aggtcaggag cccgtcgagc cgaagccccc tacccctcgg
     1141 ctggtccgag ggagctcgtc cacggccacg ggctcgaccg aggagaacgg cacgacgacg
     1201 agctgtgcga tccggtctcc tctgctgatc ttcaccggct cgtccggatc gaggttcacc
     1261 acggcgatct tgatctcgcc ccggtagccg gcgtcgatca gccccggcgc gttcgccagc
     1321 gtcaggccgg agcgggatgc cagccccgac cgcggaagga cgagaccggc gtggccctcg
     1381 gggatggcta cagcgaggcc cgtgggaaca agcgccctgc ggcccggctc caccacgagg
     1441 tccgcccagg cccggagatc gaggcccgca tcgccctcgt gggcggcgga gggaagcgga
     1501 acgtccgggt cgagacgctg gaactcgagg cgcatcaggc gcagcctacc cgcctcccgg
     1561 ccccgcgcaa agcggccgct ctttgaccac cctcgtcgcg cggtcctatg ctcagccgcg
     1621 tgtcagccaa cctctgcagc cgctgcggcc gtcccctgcg cgacggcgtc tgtccggtgg
     1681 ggcatccaca gcgcgtggct cgccggcgcc ggagacgccc gtggagggcc acgctcgcca
     1741 tcgtgatcgt catcgctctg ctcgccgggg ccgcgtacgc agcgctgatc tggtacccgc
     1801 cgagagccgc gggtgatgcg atccggcccc tgtccaccga gctcgccagg acggtgcccc
     1861 tcttccgtca gacggtcgag gcccttccca gcgacagcac gcccgaggcg ctgtccgagg
     1921 cgtccgcggt cctcacggcg gcggacgagg cacgaagcga gctgacccgg ctccagggcc
     1981 gtctggagac gacctccgtc ccctcgatcc ccgtcatctc ggaccggcca ccgatggaga
     2041 cggcctcgcg ggtccacgcg cagatgtccg acttcgcggt cggcgcgctc gagaccgtgg
     2101 gcgacctgga ggcgtcggcg cggtacctca ccgagctcgc cgcgatcctg ccctccctcg
     2161 ccaacctccg agccgcggtg gggagccccg tcgctcccgg cgaggtggag ggatccgtgg
     2221 cggcgtcacg ccccatcgcc gagcagctgc gcgcggatct ccggtcgctc acgcctcccg
     2281 aggagctgag cgagacccac gccaccctcc tggcgatcgc gcgggggctc ggcacgagca
     2341 tcgacgagct cgaagcgacc gcgggcgccg cctcggggcc ggtgatccgc gccatcgtgg
     2401 ccgacatccg ctcccagatc tcctcgttcc gggagacggc cggggcagcc ccgcaggacg
     2461 cccggacggg cgggctgtcc gatcagatcg aggacgcgga aagacgcctg gagcgcatcg
     2521 tcgccgggct ccgggagctg gaggaccagg gcgtcgagga tctcacgatc cccgacgcct
     2581 gatcgcgctc aggagcagcc gctggtcgcg ccgcaggagt tgcagacgta gcaggagccg
     2641 gccggctgca tccgcgaccc gca
//
LOCUS       DASUTZ010000188         2067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_319951, whole genome shotgun sequence.
ACCESSION   DASUTZ010000188 DASUTZ010000000
VERSION     DASUTZ010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319951"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1604)
                     /locus_tag="VF097_08350"
     CDS             complement(<1..1604)
                     /locus_tag="VF097_08350"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX6262840.1"
                     /translation="MRILTLGGGPAGLYASLLLKRLDPSHDIRIVERNPHDATYGWGV
                     VFSDRTLTEFREADYPTYRRITDDFILWDAIDIHYRGGVIRAGGQGFSGISRKKLLLI
                     LQERCGQLGIPMTFEHEFEDWGVLGDFDLVLAADGANSFTRKRFESAFRPSISQGRAR
                     FIWFGTTRPFDSFTFIFREHEHGLFQVHAYPFEGATSTFIVECHEDVWRRAGLDAATT
                     EETMAFCEDLFAAELRGHRLLSNKSEWISFPTIKTASWRHENVVLLGDSAHTAHFSIG
                     SGTKLAMEDSIALAEAFERHPEDLDAALDDYEMERRPAVERFQLAAAQSQAYFEDLRR
                     YLHMERMQFAFHLLTRSGRIDYDQLRLRDASFSDEVDRWFERDTAGQGVARFAAPPPM
                     FVPLEVRGVRLENRGVLSPAPRYSAKEGEPTPEHREALLDAARREPGLVVTEIMAVEA
                     EGRITPGDLGLYLDGHADALRDLVEAIHASSRAKVGVRIGHAGRRGSTRPRHRGLDLP
                     LGASGWPLLSASPLPYTSVSPAPREM"
     gene            complement(1601..>2067)
                     /locus_tag="VF097_08355"
     CDS             complement(1601..>2067)
                     /locus_tag="VF097_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6262841.1"
                     /translation="VWEDPSIGPRPAQDGGPELLVGGGSDLAFARVARYADGFVHGGG
                     PPRAFDRAAQRVRAAWRDAGRPEEPRLWGQSYFALGEDAAERGRDYMLDYYAFAGPFA
                     ERIAESLLTTPQDVVQQIRGYAEASCHELVMIPTVPDVEQVDRMAEIVSGMS"
BASE COUNT          305 a          709 c          753 g          300 t
ORIGIN      
        1 tccatctccc gaggcgcggg ggagacgctt gtgtacggca gcggggaagc cgagagcagg
       61 ggccagccgg aggcgcccag cggaaggtcc agcccccgat ggcgagggcg ggtggagccc
      121 ctgcgcccgg cgtgcccgat ccgaacgccg accttggccc gggagctcgc gtggatcgcc
      181 tcgacgaggt cccgcagggc gtcggcgtgg ccgtcgaggt agagcccgag gtcgccgggg
      241 gtgatccggc cctcggcctc cacggccatg atctcggtga ctacgagccc cggctcgcgc
      301 cgagccgcat cgagcagcgc ctcacggtgc tcgggggtgg gttccccctc cttggccgag
      361 taccgggggg ccggcgacag cacgccgcgg ttctcgagcc ggaccccacg cacctccagc
      421 gggacgaaca tcggcggggg cgcggcgaag cgcgccacgc cctggcccgc cgtgtcgcgc
      481 tcgaaccagc gatccacctc gtcggagaac gaagcgtcgc ggagccgcag ctggtcgtag
      541 tcgatccggc cgctgcgcgt gagcaggtgg aaggcgaact gcatccgctc catgtgaagg
      601 tagcggcgga ggtcctcgaa gtaggcctgg ctctgcgcgg cggcgagctg gaaccgttcc
      661 acggcgggcc gccgctccat ctcgtagtcg tcgagcgcgg cgtcgaggtc ctcggggtgc
      721 cgctcgaagg cctcggcgag cgcgatcgaa tcctccatcg ccagcttcgt ccccgagccg
      781 atcgagaagt gcgctgtgtg cgccgagtcc ccgagcagga cgacgttctc atggcgccag
      841 ctcgcagtct tgatcgtggg gaagctgatc cactcggact tgttggagag gaggcggtgg
      901 ccgcgcagct cggcggcgaa gaggtcctcg cagaaggcca tcgtctcttc ggtcgttgcc
      961 gcatcgagcc cggcgcgccg ccacacgtcc tcgtggcact ccacgatgaa cgtgctcgtg
     1021 gctccctcga acgggtaggc gtggacctgg aagagcccgt gctcgtgctc gcggaagatg
     1081 aacgtgaacg agtcgaacgg ccgcgtggtc ccgaaccaga tgaagcgggc ccgcccctgc
     1141 gagatcgacg gccggaaggc gctttcgaat cgcttccgtg tgaagctgtt cgcgccgtcc
     1201 gcggccagga cgagatcgaa gtccccgagc acgccccagt cctcgaactc gtgctcgaac
     1261 gtcatgggga tccccagctg cccgcagcgc tcctggagga tcagcagaag cttcttcctg
     1321 gagatccccg agaacccctg gcccccggcc cggatcacgc ccccgcggta gtggatgtcg
     1381 atggcgtccc acaggatgaa gtcgtcggtg atccggcggt aggtggggta gtcggcctcc
     1441 cggaactccg tcagggtgcg gtcggagaag accacccccc acccgtaggt ggcgtcgtgg
     1501 gggttgcgct cgacgatccg gatgtcgtgg ctggggtcca gacgcttcag caggaggctc
     1561 gcgtagaggc ccgccgggcc gccgcccaag gtgaggatcc tcacgacatc cccgagacga
     1621 tctcggccat ccggtccacc tgctctacgt cggggacggt ggggatcatc acgagctcgt
     1681 gacagctcgc ctcggcgtag cctcggatct gctggacgac gtcctggggc gtggtcagca
     1741 ggctctcggc gatccgctcg gcgaacggcc cggcgaaggc gtaatagtcg agcatgtagt
     1801 cgcggccccg ctcggcggcg tcttcgccga gcgcgaagta gctctgcccc cagagcctcg
     1861 gctcctcggg tcgtcccgcg tcgcgccacg cggcgcgcac gcgctgggcc gcccggtcga
     1921 aggcgcgagg cggaccgccg ccgtggacga atccgtcggc gtaccgggcg acgcgcgcga
     1981 aggcgagatc gctgcctcct ccgacgagga gctcggggcc gccgtcctgg gcggggcggg
     2041 gaccgatcga ggggtcctcc cagacgc
//
LOCUS       DASUTZ010000189         6109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_582181, whole genome shotgun sequence.
ACCESSION   DASUTZ010000189 DASUTZ010000000
VERSION     DASUTZ010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6109
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582181"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VF097_08360"
     CDS             complement(<1..205)
                     /locus_tag="VF097_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262842.1"
                     /translation="MSGLLSAGPAQDRWAGAFVSAPAGTMRASPSRDAAGRRASMDGF
                     RDWLEGLTARELAVYGVVALALLV"
     gene            204..845
                     /locus_tag="VF097_08365"
     CDS             204..845
                     /locus_tag="VF097_08365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEX6262843.1"
                     /translation="MDIVNPEVERYLRELLDEDHPLAAEMEARAEEHDFPIVGRVVGQ
                     TMEVLARSIGARRVFEMGSGYGYSAYWFSRAVGPKGEVHMTEGDPENQRLALEYLERA
                     GFADRVSSHVGDALELIEETPGEFDVVFCDIDKHGYPEAWSKARERIRVGGLYLCDNV
                     LWSGRVAQKDPEDPRPEWTAAIREHNAAVAGDERYRSTIVPTRDGLTVALRVA"
     gene            complement(931..3360)
                     /gene="leuS"
                     /locus_tag="VF097_08370"
     CDS             complement(931..3360)
                     /gene="leuS"
                     /locus_tag="VF097_08370"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX6262844.1"
                     /translation="MNEYRPQEIEPRWVDAWEEDRLYEAGGEEDPRPRFYALDMFPYP
                     SGDIHMGHAEAFAIGDVVARFHRMRGQNVLHPIGWDAFGLPAENAAIQRGIHPKTWTY
                     ENVDKQAASFKRYGVSFDWARRFNTCDPEYYRWTQWLFLKLHERGLAYRKLAPANWCP
                     KDQTVLANEQVVNGACERCGTPVVRKDLTQWFLRITDYAEGLLDDMELLEDWSDRVLT
                     MQRNWIGRSEGAEVEFEIAETGDRVTVFTTRPDTLWGVTFMVFAPEHELVPALAERGG
                     ASGQAAELLERVRSTPLASRDLGESREGIELGVHAVNPVNGEKVPIFVAPYVLMEYGT
                     GGVMGVPGHDQRDFEFAREHGLEVRVVIQPEGEELDAEEMTEAYPHEGVMVRSGGFDG
                     TPSPESIGRVTAWLEETGRGRPAVTYRLRDWLISRQRYWGAPIPIIHCPECGEVPVPE
                     EDLPVLLPEDVDFAPTGESPLAEHEGFVNVSCPRCGGAARRETDTMDTFVDSSWYYLR
                     YCSPHEEDRPFDSEAVERWMPVGQYTGGIEHAILHLLYSRFLTKVLHDMGMVSFREPF
                     PRLLNQGMVIMQGAKMSKSRGNLVEPHPLIERWGADTLRVTMLFAGPVEDDVDWATVS
                     PAGVHSWLGRVWRVGLAVADQGGEDPEDLRRLTHRTIRDVTSLYERFRTNVAVARLME
                     LTNEIRRVLDEGGSARQAAEALVLMLAPMAPFVTEEIWRGTWGRRESVHVQDFPSFDP
                     SLAREETVMLVVQVDGKVRDTIEVPADVDEEAAVELARASEKVRRALDGREVSRVIAR
                     PPRLVNLVTAV"
     gene            3465..4664
                     /locus_tag="VF097_08375"
     CDS             3465..4664
                     /locus_tag="VF097_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262845.1"
                     /translation="MGKDISADESKSKHASEPEVIPRGNGDRAVPAPTPEPVEAHRGR
                     RRRWPVLANKVGHFLAAFFLLVLSIELMKGGAEAIAPRLQGTFPVDNMVSTMGLGWLG
                     AYLVLSGSPVAAASLALFTAGATTQAQTFTMLSGSRLGASFIVLLVGFLYAMRQRNRH
                     RRRESIGMGVLALGMTAVVYVPGMFLGYGILKSGLLSGVSWRSSAGINGIVDAVWGPI
                     TRFLSELLPGPLLFPLGLAVILASFKLLDFVLPALDGEKHAGERGHWMKHPWAMFGLG
                     MLAALLTLSVSVALTILVPLASKGYIERREAIPYIAGANITTLADTLFAAMLLGNPIA
                     VQIVLAQAIAVAIVTLLLLAFLYRPLQTTIMALDEWIVRTTPRLVAFVAILFVMPIGL
                     LFSGRML"
     gene            complement(4669..5970)
                     /locus_tag="VF097_08380"
     CDS             complement(4669..5970)
                     /locus_tag="VF097_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262846.1"
                     /translation="MRRFAVLVAALLALTGLLATPSARAAPQTPPFGPLIEGHAVYEG
                     QSRCAPKEKPGVTAFRKLLVSTYGETWMNSSRPCNVGGKSEHKEGRALDWAMDASKPR
                     DRKKVKDLFDWMFAEDARGNINAMARRLGLMYVIWNRRWWTSWKGTWKRYCVQKPRGC
                     RTPSGSFTHPHDDHVHFSFSWQGARKRTSFFTPERSFITGMAASSGGGYWLAGGDGSV
                     RAYAAPFHGSKAGKFPPAPIVEMASRPDDDGYWLVLRSGRVLAFGEAGLHGRVTRKKA
                     VIVGFGATATGEGYWLLTKQGKVAAFGDAGRYGRVEKGAAAVTGIVPTPSGQGYWVYA
                     AGGEVFAFGDAEHQGDLADASLAAPIVGGAASGPDGYWLVTRKGNVHAFGSAQLHGQA
                     ANKGLGPVAAIAASPSGSGYWVVGRTGRVAAFGDAKLRGSL"
BASE COUNT          888 a         2267 c         2017 g          937 t
ORIGIN      
        1 ccacgagcag cgccagcgcg acgaccccgt acaccgcgag ctccctcgcg gtcagcccct
       61 ccagccagtc gcggaacccg tccatggaag cgcggcgtcc ggccgcgtct cgggaggggg
      121 aggcccgcat cgtaccggcc ggcgccgaca cgaacgcccc ggcccaccta tcctgtgccg
      181 gtcccgccga gaggagcccc gacatggaca tcgtgaatcc cgaggtggag cggtacctgc
      241 gcgagctgct ggacgaggac caccccttgg ccgccgagat ggaggcccgg gccgaggagc
      301 acgatttccc gatcgtgggg cgcgtggtgg gccagaccat ggaggtcctg gcccgctcga
      361 tcggcgcccg ccgcgtcttc gagatgggct ccggctacgg ctactccgcc tactggttct
      421 cacgagcggt cggccccaag ggcgaggtcc acatgacgga gggcgacccc gagaaccagc
      481 gcttggcgct cgagtacctg gagcgagccg ggttcgcgga tcgagtcagc tcccacgtcg
      541 gcgacgccct cgagctgatc gaggagaccc cgggcgagtt cgacgtcgtc ttctgcgaca
      601 tcgacaagca cggctacccc gaggcctgga gcaaggcgcg ggagcggatc cgcgtgggcg
      661 gcctctacct ctgcgacaac gtgctctggt cgggccgggt ggcccagaag gatcccgaag
      721 acccccgtcc cgagtggacc gccgcgatcc gggagcacaa cgccgccgtg gcgggagacg
      781 agcgctaccg ctcgacgatc gtccccacgc gggacggcct gacggtcgcc ctccgcgtgg
      841 cctgagtcat cgcttgccgc tcggcttggc cccggggtcc gcgaagagtc gcaatcgtcc
      901 tctgctgcac ctatccagcg aggggacggc tcacacggcc gtcacgagat tcaccaagcg
      961 gggcggccgg gcgatgacgc gagacacctc gcgtccatcc agggcccgcc gcaccttctc
     1021 tgaggcccgg gccagctcga ccgccgcctc ctcgtccacg tcggccggca cctcgatcgt
     1081 gtctcgcacc ttcccgtcca cctggacgac gagcatgacg gtctcctccc gggccaggct
     1141 gggatcgaac gaggggaagt cctggacgtg gacgctctcc ctccggcccc atgtcccccg
     1201 ccagatctcc tcggtgacga acggagccat cggggcgagc atcagcacca gggcctcggc
     1261 ggcctgccgc gcgctccccc cctcgtccag cacccgccgg atctcgttcg tgagctccat
     1321 cagccgcgcc acggccacgt tcgtccggaa ccgctcgtag aggctcgtga cgtcgcggat
     1381 cgtgcggtgc gtcaggcgcc ggaggtcctc cggatcctcg cccccctggt cggccacggc
     1441 caggcccacc cgccagacgc gtcccagcca cgaatggact cccgccggcg agacggtcgc
     1501 ccaatcgacg tcgtcctcga cgggcccggc gaacagcatc gtcacgcgga gggtgtcggc
     1561 tccccaccgt tcgatcaagg gatggggctc cacgaggttc ccccgcgact tcgacatctt
     1621 ggccccctgc atgatgacca tgccctggtt cagcagccgg gggaagggct cgcggaacga
     1681 caccatcccc atgtcgtgca gcaccttggt gaggaaccgc gagtacagga ggtggaggat
     1741 cgcgtgctcg atccctccgg tgtactggcc gaccggcatc caccgctcca cagcctcgga
     1801 gtcgaacggg cggtcctcct cgtgggggga gcagtaccgc aggtagtacc aggaggagtc
     1861 cacgaacgtg tccatcgtgt ccgtctcgcg ccgcgcggcg ccgccgcagc gcggacacga
     1921 cacgttcacg aacccctcgt gctcggccag gggcgactcg cccgtcgggg cgaagtccac
     1981 gtcctcgggg aggaggaccg ggaggtcctc ctccggcacc ggcacctcgc cgcactcggg
     2041 gcagtggatg atcgggatcg gcgcgcccca gtaccgctgc ctggagatca gccagtcgcg
     2101 gagccggtac gtcaccgcgg gccgccctcg tcccgtctcc tcgagccagg cggtcacccg
     2161 gccgatcgac tccggagacg gcgtcccgtc gaaccctccg gaacggacca tgacgccctc
     2221 gtggggatac gcctcggtca tctcctcggc gtcgagctcc tcaccctccg gctggatcac
     2281 cacgcgcacc tcgagaccgt gctcgcgggc gaactcgaag tcccgctggt cgtgcccggg
     2341 cacccccatc accccgccgg tcccgtactc catcaggacg tagggagcca cgaagatcgg
     2401 caccttctcg ccgttcacgg ggttcacggc gtgcacgccc agctcgatgc cctcgcggga
     2461 ctcgcccagg tctcgcgagg cgagcggcgt ggagcgcacc cgctccagca gctcggcggc
     2521 ctggcccgac gctccccccc gctcggcgag cgcgggcacc agctcgtgct cgggcgcgaa
     2581 gaccatgaac gtcacgcccc agagcgtgtc cgggcgggtc gtgaagacgg tcacccggtc
     2641 gcccgtctcg gcgatctcga actcgacctc tgccccctcc gagcgcccga tccagttccg
     2701 ctgcatcgtc aggactcggt cggaccagtc ctcgagcagc tccatgtcgt cgaggagccc
     2761 ctccgcgtaa tcggtgatcc ggaggaacca ctgcgtgagg tccttccgca cgacgggggt
     2821 tccgcagcgc tcgcaggcgc cgttcacgac ctgctcgttg gccagcaccg tctggtcctt
     2881 ggggcaccag ttcgcgggcg cgagcttccg gtaggcgagg ccccgctcgt ggagcttcag
     2941 gaacagccac tgggtccagc ggtagtactc cgggtcgcag gtgttgaaac gacgggccca
     3001 gtcgaacgac acgccgtacc gcttgaacga cgcggcctgc ttgtcgacgt tctcgtaggt
     3061 ccacgtcttc ggatggatcc cccgctggat cgcggcgttc tccgccggca gcccgaacgc
     3121 atcccacccg atcgggtgca ggacgttctg tcctcgcatc cggtggaacc tggccaccac
     3181 gtcgccgatg gcgaacgcct ccgcgtggcc catatggatg tcgccggacg ggtacgggaa
     3241 catgtcgagc gcgtagaagc ggggacgagg atcctcctcg cctcccgcct cgtagagacg
     3301 atcctcctcc caggcgtcga cccacctggg ctcgatctcc tgtggtcggt actcgttcat
     3361 ggagccttcc gccgggtaga gcagggaagg gagccggaac cattgtcacc ccgacgcccc
     3421 gccgatatcg tatcgtgagc cccggcccct gatggccccg agcaatgggg aaggacatct
     3481 ccgccgacga gtcgaagtcg aagcacgcat ccgagcccga ggtcatcccc cgcgggaacg
     3541 gggatcgggc cgtgcccgcc ccgacgccgg aaccggtcga ggcccaccgc ggacggcgtc
     3601 ggcgctggcc cgtgctggcc aacaaggtgg gtcacttcct cgcggcgttc ttcctcctgg
     3661 tgctgtcgat cgagctgatg aagggtggag ccgaggccat cgccccccgg ctccagggaa
     3721 ccttccccgt cgacaacatg gtcagcacga tggggctggg ctggctgggc gcctacctgg
     3781 tgctgtccgg atcgcccgtc gcggccgcct cgctcgccct cttcacggcc ggggccacga
     3841 cccaggccca gaccttcacc atgctctccg gctcacggct gggcgcctcg ttcatcgtcc
     3901 tcctcgtcgg gttcctctac gcgatgcgcc agcgcaaccg gcatcgaagg cgggagagca
     3961 tcgggatggg cgtcctggcc ctggggatga cggccgtcgt gtacgtgccc gggatgttcc
     4021 tcgggtacgg gatcctcaag tcgggcctcc tctcgggcgt ctcgtggcgc tcctcggccg
     4081 ggatcaacgg gatcgtggac gccgtctggg ggccgatcac gcgcttcctg tcggagctgc
     4141 tgccggggcc gctgctcttc cccctgggcc tggccgtcat cctcgcgtcg ttcaagctgc
     4201 tcgacttcgt gctgccggcg ctcgacggcg agaagcacgc cggggagcgc ggccactgga
     4261 tgaagcaccc gtgggcgatg ttcgggctcg ggatgctcgc cgccctgctc accctgtcgg
     4321 tgtcggtggc gctcacgatc ctcgtcccgc tggcgagcaa gggctacatc gagcgccgcg
     4381 aggcgatccc ctacatcgcc ggcgcgaaca tcaccacgct cgccgacacg ctcttcgcgg
     4441 ccatgctgct agggaacccc atcgccgtgc agatcgtgct ggcgcaggcg atcgcggtgg
     4501 ccatcgtcac gctgctcctc ctggcgttcc tctaccgtcc gctccagacg acgatcatgg
     4561 cgctggacga gtggatcgtg cgcaccacgc cccgcctcgt cgcgttcgtg gcgatcctct
     4621 tcgtgatgcc gatcggcctg ctcttcagcg ggaggatgct ctgacggctc acagggagcc
     4681 gcggagcttg gcgtccccga aggccgcgac cctccccgtc cgccccacga cccagtagcc
     4741 ggagcccgag ggcgacgcgg cgatggcggc gaccgggccc aggcccttgt tcgcggcctg
     4801 gccgtgcagt tgggccgagc cgaaggcgtg gacgttgccc ttgcgggtca cgagccagta
     4861 tccgtccggg ccgctcgcgg ccccgcccac gatcggcgcg gcgagggacg catcggccag
     4921 gtccccctga tgctccgcgt ctccgaaggc gaagacctcg cccccggccg cgtagaccca
     4981 gtagccctgt cccgacggcg tagggacgat cccggtgacg gctgccgccc ccttctccac
     5041 ccggccgtag cggcccgcgt cgccgaaggc cgcaaccttc ccctgcttgg tcaggagcca
     5101 gtacccctcg cccgtcgccg tggccccgaa cccgacgatg accgccttct tccgcgtcac
     5161 ccggccgtgg agcccggcct cgccgaaggc cagcacgcgg cccgaccgga ggacgagcca
     5221 gtacccgtca tcgtccggcc gggaggccat ctccacgatg ggcgccggcg ggaacttccc
     5281 cgccttggag ccgtggaacg gcgcggcgta ggcccgcacg ctcccgtccc ctcccgccag
     5341 ccagtacccg ccgccgctcg aggccgccat ccccgtgatg aaggaccgct ccggggtgaa
     5401 gaagctcgtt cgcttccggg ctccctgcca cgagaagctg aagtggacgt ggtcgtcgtg
     5461 cggatgggtg aaggagcccg acggcgtcct gcatccccga ggcttctgga cgcagtaccg
     5521 cttccaggtc cccttccagg acgtccacca cctgcggttc cagatcacgt acatcagccc
     5581 gagcctccgg gccatcgcgt tgatgttccc ccgggcatcc tccgcgaaca tccagtcgaa
     5641 gagatccttc accttcttac ggtctctggg cttcgaggcg tccatggccc aatcgagggc
     5701 ccgcccctcc ttgtgctcgc tcttcccgcc tacgttgcag ggacggctcg agttcatcca
     5761 cgtctcgccg taggtggaaa cgaggagctt ccggaacgcc gtgacgccgg gcttctcctt
     5821 gggagcgcag cggctctggc cttcgtacac ggcgtggccc tcgatcagcg ggccgaaggg
     5881 gggtgtttgc ggcgctgctc gcgccgaggg ggtcgcaagg agtcccgtca gcgcgagcag
     5941 cgccgcgacg agcacggcga agcgtcgcat aggagtgatg tccgcaggca tggggttccc
     6001 cctgtcctac ggcatcactc ggaggatcgc aagcccgact tgagccgggc gcggcctcag
     6061 aagatgctga cgccgccggg cccgatgagg aggcccagga cgatgagca
//
LOCUS       DASUTZ010000190         7638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_63587, whole genome shotgun sequence.
ACCESSION   DASUTZ010000190 DASUTZ010000000
VERSION     DASUTZ010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7638
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63587"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..1464
                     /locus_tag="VF097_08385"
     CDS             208..1464
                     /locus_tag="VF097_08385"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX6262847.1"
                     /translation="MIRSLDELADRTPATRDRVMDFLRAFSIATVVLGHWLIAILWWE
                     DGLLYQTSAIGETSGLWLATWFLQVMPIFFFVGGFANMITYDAYKRRGDSTWAFVRSR
                     VERLLRPSLVFLGLWLAVQVALHLADVGRPTGPVLWGDTRLLRGMNPPPGTLPFGPLW
                     FLAVYLVVVAISPATIWLHRRFRWWVVAILAAGPFVVDLFGFGFDMYRLRYLNIPFVL
                     LLPHQLGHFYADGTLRRLPRSVYWAMAIGGLAGLVLATNPWLFQWLGGERRFEWFPTI
                     GYYPKSALGTDAEPISNAFPPTIVFLLVGVWTIGAVMLLRHRLAPWLERRGPWKATIL
                     GNSVIMTLFLWHMTAYLLAILLLWPMGFGREHDSTLRWWLERPLWILVPGLILLGIVT
                     LVGRFERGATRPAEAPAPPEHPLATA"
     gene            complement(1469..4105)
                     /locus_tag="VF097_08390"
     CDS             complement(1469..4105)
                     /locus_tag="VF097_08390"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6262848.1"
                     /translation="MSDLPTGTVTFLFTDIEGSTRLLQDLGPGYRRVQDRHDELLLEA
                     IQAEGGRVVRIEGDSAFSVFVNPTQAVRAAVAAQRSLAREPWPHGEALRVRMGLHTGE
                     GTPGGGDYVGIDVNRAARIAAAGHGGQVLLSEATRGLIDHALPEGVIVRDLGTHRLKD
                     IDRPERLHDLVVEGLPADFPPLRTLDHRSTNLPAERSSFVGREGDLARIEELLAGSRL
                     VTLVGPGGIGKTRMALRAAAQHLDRFDDGVFLVDLSGVASPDLVPSAIAASLRIPQEP
                     SRELLDSIAERLRERSLLLVLDNFEHVVAAAPSVEHLLGAAPELRVLATSRLPLGLAG
                     EQEYPVEPLPLPGPDEAADVEALTVNDSVKLFVDRAAQVHPGFAITGRNAAPIGEIVS
                     RLDGLPLAIELAASRMKVLSPSELLGRLGRRLPLLTGGARGAPERHRTLRAAIEWSHE
                     LLESEERRLFARLAVFAGGWTLESAEEVCGAGLRIEVLQGLSSLVDQSLVGRGHLRDE
                     GTRFRMLETIREFAAERLATSGEESDIRRRHGEWMRDLALEAGPHLVGPAQARWLARL
                     ETEHGNLRAALEWAEATGDAETGLRIAASVWRFWHRRGHLDEAMSRLTRLLAMPQGQR
                     RDAVRARALGALGGIAYWQADYGAMGSAYEEAVEIARELGDRDLEAHALFDAAFIPVV
                     KNDHEGMETAFQAALRLAEEVGDRSLQANAWAALARGRLFGGRAAEAADLLHRAISLR
                     GDQGDPQAVAEDYEVLAHIEGTGGAMARAKTHIAAAMRLHLAAGNVLGLALSMRPLAI
                     LASIEGEHGRAVRLLGASARLLEDVGGISPPMGFLMLRDVEATARDALGAGPYRRAWE
                     DGYASSFDDAVAMAMVGLPETP"
     gene            complement(4257..4646)
                     /locus_tag="VF097_08395"
     CDS             complement(4257..4646)
                     /locus_tag="VF097_08395"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX6262849.1"
                     /translation="MAGDENQRIVERYWAALATGDFDDAAAFLSDDFVEDWPQSGERI
                     IGPANWLEMARHHPTFPSIEPVRHEGQGDLWVSELLFDYGRGGESQPYHVCAVQHLRE
                     GRIASIVEYFAAPFEPADWRIDWVERT"
     gene            complement(4658..5146)
                     /locus_tag="VF097_08400"
     CDS             complement(4658..5146)
                     /locus_tag="VF097_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262850.1"
                     /translation="MYRWWVFLHVVGGFGFFMAHGASAMASIRLRPERSPERVKALLD
                     LSSSSLSFMYVSLLLLLIGGIVSGFLGDWWGEGWIWTAIGLLILIMGAMYGLASNYYN
                     QVREAAGIQTYQQRKKGIEAAPPDPGRLDALLSSSRAVVIMVVGFGGLLLILWLMILK
                     PF"
     gene            5192..7219
                     /locus_tag="VF097_08405"
     CDS             5192..7219
                     /locus_tag="VF097_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262851.1"
                     /translation="MAPRLKHRLDDVVRALVTPRAEPGALPLVDAAARFRPDDRSAVS
                     SSSAFLLALAGSGRAWEHLSAAAEGGDAVAAFLADGLRRIEDEVEGLVGREPALAERL
                     EALAGFLEREDAGSSVDELAERIWSVFFPEGVGIRGHEEERAAELRARRAIDINGPGP
                     AVTDAARQVLFTVNLLLTGPPAGVEVDDLALPSEITEALRDPGSQMFWYDHPVQIGID
                     APSSELRYGLMAMDRGMAFERERGAHRPLRVVVSLSTTHVALEGIARPYLEHEVREMG
                     GLEHLELYAFGEADAERLVAEVLAPAAEVFLDRPDARELLGVLGVRGPYGRHHSFGKA
                     IAAIWHVLIDPEVAATFKTDLDHSWPQNELVSETGASLLEHFAFPLWGATGVDAEGRR
                     VELGGFAAALVNEADIDQGLFTIDVPVPDRPPEADELVFWSALPQGLSTLAEASTRYG
                     IDGPDGLTTAIERVHVHAGTVGILVEALRRHRPFTPSFVGRAEDQAYILSTLWESEPR
                     LAHVHHRELIQRHDKKAFAGAAIEAARVGTWVGDHERLLLFSELARHLGPGREEVKSV
                     AGPFTGAFISEIPVTVTLLRLTVKAGALAANGAGVEAEELLAMAAERLGRRLAFVEGG
                     ELAERLDAERRGWDLLYETLGELERRLAAGDPEAERLTDRARRVVAACAVG"
     gene            complement(7227..>7638)
                     /locus_tag="VF097_08410"
     CDS             complement(7227..>7638)
                     /locus_tag="VF097_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX6262852.1"
                     /translation="GGAGRDRLDGGFGADVFRGGPGRDTVSYVGRTAKVDVVLDDVAN
                     DGYITGEGDNVRTDVENAIGGDGPDGLVGSALVNVFHGGPGEDNLFGGDGNDRLFGEG
                     GDDFILGQDGDGDLANGGPGTDDCDAENEIGCEV"
BASE COUNT         1051 a         2693 c         2737 g         1157 t
ORIGIN      
        1 ctcgcgctgg ccgtcggtct gaccggctgg ggagcgaagg ccagcctgac cgccatggtg
       61 atcatcctcc tgatcccggt ggcggcgggc atgatcgtgt ggcgcaggca gaccgagccg
      121 gatccgttcg gtctcgcctg agacgggctc gacgtcggcg tctgcgagcg cgaacccccc
      181 aacggggagc gatagtctga gcgagtcatg atccgctcgc tggacgagct cgccgatcgg
      241 acgcccgcca cccgcgatcg ggtcatggac ttcctgcgcg cgttcagcat cgccacggtg
      301 gtgctcgggc actggctgat cgcgatcctc tggtgggagg acgggctcct ctaccagacg
      361 agcgcgatcg gggagacctc ggggctctgg ctcgccacgt ggttcctgca ggtgatgccg
      421 attttcttct tcgtcggtgg gttcgccaac atgatcacct acgatgcgta caagcggcgc
      481 ggggactcca cgtgggcgtt cgtcaggagc cgggtggagc ggctgctccg tccctcgctc
      541 gtcttcctcg ggctctggct cgccgtgcag gtggcgctgc acctggccga cgtgggtcgg
      601 cccaccggtc ccgtgctctg gggcgacacg cggctgcttc gggggatgaa cccgccgccc
      661 ggaactctcc cgttcgggcc gctctggttc ctggccgtgt acctcgtcgt cgtggcgatc
      721 agcccggcga cgatctggct ccaccgccgc ttccgctggt gggtcgtggc catcctggcg
      781 gccgggccgt tcgtggtgga cctcttcggg ttcggcttcg acatgtatcg cctgcggtac
      841 ctcaacatcc cgttcgtgct gctgctgccc caccagctcg ggcacttcta cgccgacggg
      901 acgctgcggc gcctcccccg gagcgtgtac tgggcgatgg cgatcggtgg gctggcgggg
      961 ctcgtgctgg ccacgaaccc gtggctcttc cagtggctgg ggggagaacg gcggttcgag
     1021 tggttcccca cgatcggcta ctacccgaag agcgcgctcg gcaccgacgc cgaaccgatc
     1081 tcgaacgcgt tcccgcccac gatcgtcttc ctcctcgtgg gggtctggac gatcggggcg
     1141 gtgatgctgc tgcgccaccg gctggccccg tggctggagc ggcgcggccc gtggaaggcg
     1201 acgatcctcg ggaacagcgt aatcatgacg ctcttcctct ggcacatgac cgcctacctc
     1261 ctggcgatcc tgctgctgtg gccgatgggg ttcgggcggg agcacgacag caccctgcgg
     1321 tggtggctgg agcggccgct gtggatcctc gtccccggcc tgatcctgct cggcatcgtc
     1381 acgctggtgg ggcgcttcga gcggggggcg acccgcccgg cggaggctcc cgcgccaccc
     1441 gaacacccgc ttgcgacggc gtgaccgttc atggtgtctc cggcaggccc accatcgcca
     1501 tcgccaccgc gtcgtcgaag gacgaggcgt atccgtcctc ccaggcccgt cggtacggcc
     1561 cggccccgag cgcgtcccgg gcggtagcct ccacgtcgcg cagcatgagg aatcccatgg
     1621 gcggggatat cccaccgacg tcctcgagca gccgggccga ggctccgagg aggcgcaccg
     1681 cccgcccgtg ctcgccctcg atcgacgcga ggatcgcgag cggccgcatc gagagcgcca
     1741 gcccgagcac gttcccggcc gccaggtgga gtcgcatcgc cgctgcgatg tgcgttttcg
     1801 cacgggccat ggctccaccg gtcccttcga tgtgggcgag gacctcgtag tcctcggcca
     1861 ccgcctgagg atccccctgg tcccctcgga gggagatggc tcggtggagg agatcggcgg
     1921 cctccgccgc gcgtccgccg aagagtcgac cccgagccag ggcggcccag gcgttggcct
     1981 ggagcgatcg atcgccgacc tcctccgcca ggcggagagc cgcctggaat gccgtctcca
     2041 tgccctcatg gtcgttcttc accaccggga tgaacgcggc gtcgaagagg gcgtgggcct
     2101 cgaggtcccg atccccgagc tcccgggcga tctccaccgc ctcctcgtag gcggacccca
     2161 tcgctccgta gtcggcctgc cagtacgcga tcccgccgag ggcgccgagg gcccgggccc
     2221 ggaccgcgtc tcgacgctgg ccttgtggca tggcgagcaa ccgggtcagg cgcgacatgg
     2281 cctcgtccag atggccgcgg cgatgccaga agcgccagac ggaagcggcg atccgaagcc
     2341 cggtctccgc gtcgccggtg gcctcggccc actcgagagc ggctcggagg ttgccgtgct
     2401 ccgtctcgag gcgggccagc caccgggcct gggccggccc caccaggtga gggccggcct
     2461 caagggccag gtcccgcatc cactccccgt ggcgacgacg gatgtcggac tcctcgccgg
     2521 aggtggccag acgctcggcg gcgaactcgc ggatggtctc cagcatcctg aaccgggtcc
     2581 cctcgtcgcg aaggtgacct ctcccgacca ggctctggtc cacgagggag ctcagcccct
     2641 gcagcacctc gatccggagg ccggccccgc agacctcctc cgcgctctct agggtccagc
     2701 cgccggcgaa caccgcgagg cgagcgaaga ggcgccgctc ctcggactcg agcagttcgt
     2761 ggctccattc gatggcggcg cggagggtgc gatgacgctc cggggcgccg cgggcgccac
     2821 cggtcaggag ggggagtcgc cggcccagcc gccccaggag ctcggaaggg ctcaggacct
     2881 tcatccggct cgcggcgagc tcgatggcca gcgggagccc gtcgagacga gaaacgatct
     2941 cgccgatcgg ggcggcgttc cgtccggtga tcgcgaagcc tgggtgcacc tgcgccgcgc
     3001 ggtccacgaa gagcttcacc gagtcgttga cggtgagcgc ttcgacatcg gcggcctcgt
     3061 ccggccccgg caggggaagc ggctccaccg ggtactcctg ctctccggcg agcccgagcg
     3121 ggagccggct cgtggccagc acccgcagct cgggggccgc cccgaggaga tgctcgacgc
     3181 tgggggcagc cgcgacgacg tgctcgaagt tgtccaacac cagaaggagg ctccgctctc
     3241 tcagccgctc ggctatcgaa tcgagaagct cccgggaggg ctcctgtggg atgcggaggg
     3301 atgcggcgat cgccgagggg accaagtcgg ggctcgccac cccgctgagg tcgacgagga
     3361 acaccccgtc gtcgaaccgg tcgaggtgct gggccgccgc ccgcagggcc atcctcgtct
     3421 tcccgatgcc gcccgggccc acgagcgtca cgagacggct cccggccagc agctcctcga
     3481 tcctcgccag gtccccttcc cggccgacga aagagctccg ctcggcgggg aggttggtgg
     3541 agcggtggtc gagggtccgc agcggcggga agtccgcggg gagaccctcg acgaccaggt
     3601 cgtggagacg ctccgggcgg tcgatgtcct tgaggcggtg ggtgccgagg tcccggacga
     3661 tcacgccctc cggaagggcg tgatcgatca gccctcgggt tgcctccgag aggagcacct
     3721 gtccgccgtg gcccgcggcg gcgatcctgg ccgcacggtt gacgtcgatc cccacgtagt
     3781 cgccccctcc gggggtcccc tctcctgtgt gcaggcccat gcgcacccgg agggcctccc
     3841 catggggcca gggctcccgg gccaggctcc tctgagcggc cacggctgcg cgcaccgcct
     3901 gcgtggggtt cacgaacacc gagaaggcgg agtcgccctc gatgcgcacc acccgccctc
     3961 cctcggcctg gatggcttcg aggaggagct cgtcgtggcg gtcctgcacc cggcggtacc
     4021 ccggtccgag atcctggagc agccgcgtgg agccctcgat gtccgtgaag aggaaggtga
     4081 cggtcccggt ggggagatcg ctcatcgttc gagcgagcgc gcgtcggagg tgggagaggt
     4141 accgctcatc ctccgaggag cacgcctcct ccgttggggc cggcgtaacg accctacacg
     4201 gctggtcggc gccgtgggag aaggatgcga cgtcctcgat ccacggctcg ctccccctac
     4261 gtccgctcca cccagtcgat gcgccagtcg gcgggctcga agggcgcggc gaagtactcg
     4321 acgatcgaag cgatcctgcc ctcgcggagg tgttggaccg cgcagacgtg atagggctgg
     4381 ctctcgccgc cccgcccgta gtcgaagagc agctcgctca cccagaggtc gccctgaccc
     4441 tcatgtcgca cgggctcgat cgaggggaag gtggggtggt gacgcgccat ctccagccag
     4501 ttcgcgggcc cgatgatcct ctcgccggac tgaggccagt cctctacgaa gtcgtcgctg
     4561 aggaaggccg cggcatcgtc gaagtctccg gtcgccagtg ccgcccagta gcgctccaca
     4621 atccgctggt tctcgtctcc cgccatgctt cctcccctca gaacggcttg aggatcatca
     4681 gccacaggat caggagcagg ccgccgaagc ccaccaccat gatcaccacg gcccgcgagg
     4741 acgagaggag ggcatccagg cggccgggat cgggaggtgc ggcctcgatg cccttcttcc
     4801 tctgctggta ggtctggatc ccggccgcct cccgcacctg gttgtagtag ttcgaggcga
     4861 gcccgtacat cgcccccatg atgaggatca gcagcccgat ggccgtccag atccatccct
     4921 cgccccacca gtcccccagg aagccgctga cgatgccccc gatcagcagc agcagcaggg
     4981 agacgtacat gaagctcaga ctcgaggacg agagatcgag gagggccttg acccgctcgg
     5041 ggctccgttc gggccggagc cggatcgacg ccatcgccga cgctccgtgc gccatgaaga
     5101 acccgaaccc cccgaccacg tgcaggaaca cccaccagcg gtacatcttg cctccctcgt
     5161 tggctctcgg cggggagggt agcatcgctg cgtggcgccg cgcctcaagc atcggctcga
     5221 cgacgtcgtc cgggcactcg tgacgccgcg agccgagccc ggagctctcc cgctggtcga
     5281 cgcggccgcc cggttccggc ccgacgatcg gtccgccgtc tcctccagct ccgccttcct
     5341 cctggcgctc gcagggtcgg gccgggcctg ggagcacctg tccgcagcgg cagagggcgg
     5401 cgacgccgtg gccgcgttcc tggcggacgg cctccggagg atcgaggacg aggtggaggg
     5461 tctcgtgggc cgggagcccg cgctggccga gcggctggag gcgctggccg ggttcctgga
     5521 gcgggaggac gccggaagct cggtggacga gctggccgag cgcatctggt ccgtgttctt
     5581 ccccgagggc gtggggatcc ggggtcacga ggaggagcgg gccgcggagc tgcgggctcg
     5641 acgggccatc gacatcaacg ggcccggccc ggccgtgacg gacgccgcac gccaggtcct
     5701 gttcacggtc aacctgctgc tgaccggacc gcctgccggc gtggaggtgg acgacctggc
     5761 tctcccgagc gagatcacgg aggccctgcg ggatccgggt tcgcagatgt tctggtacga
     5821 ccacccggtc cagatcggca tcgatgctcc ttccagcgag ctccgatacg ggctgatggc
     5881 catggaccgg gggatggcct tcgaaaggga gcggggggcc caccggcccc tgcgggtcgt
     5941 ggtctcgctg tccacgacac acgtggcgct cgaggggatc gcccgcccct acctcgagca
     6001 cgaggttcgc gagatgggtg ggctggagca cctcgagctg tacgcgttcg gggaggccga
     6061 tgcggagcgg ctcgtggcgg aggtgctggc cccggcggcc gaggtgttcc tcgaccggcc
     6121 cgatgccaga gagcttctgg gggtgctcgg cgtgcgcggg ccctacgggc gccaccacag
     6181 cttcgggaag gcgatcgcgg ccatctggca cgtgctgatc gatcccgagg tggcggcgac
     6241 gttcaagacg gatctcgacc acagctggcc ccagaacgag ctcgtctcgg agacgggggc
     6301 ctcgctgctg gagcacttcg ctttcccgct gtggggcgcg accggggtgg acgcggaagg
     6361 gcgccgggtg gagctcggag ggttcgcggc ggcgctggtc aacgaggcgg acatcgacca
     6421 ggggctgttc acgatcgacg tgcccgttcc cgaccggcca cccgaggccg acgagctcgt
     6481 cttttggagc gcgctgcccc agggtctctc gaccttggcc gaggcctcga cgcgatacgg
     6541 gatcgacggc cccgacggct tgaccacggc gatcgagcgg gtccacgtcc acgccggcac
     6601 ggtggggatc ctggtggagg ccctcaggcg gcaccgaccc ttcacgccgt ccttcgtcgg
     6661 ccgggccgag gaccaggcgt acatcctctc caccctctgg gagtccgagc cgaggctggc
     6721 gcacgtccac caccgcgagc tgatccagcg acacgacaag aaggcgttcg cgggcgcggc
     6781 catcgaggcc gctcgcgtgg ggacctgggt cggggaccac gagcggctgc tgctcttctc
     6841 cgagctggcg cggcacctgg gcccgggccg ggaggaggtg aagtcggtgg ccgggccctt
     6901 cacgggtgcc ttcatctcgg agatccccgt cacggtcacc ctcctgcggc tgacggtgaa
     6961 ggcgggggcc ctggcggcga acggggccgg ggtcgaggcc gaggagctgc tcgcgatggc
     7021 ggcggagcgg ctggggcgga ggctggcgtt cgtggagggg ggcgagctcg ccgagcgtct
     7081 cgacgccgag cgccgaggat gggacctgct ctacgagacg ctcggcgagc tcgagcggcg
     7141 cctggcggcg ggggatcccg aagcggagag gctgaccgac cgcgcacgcc gcgtggtcgc
     7201 ggcgtgcgcg gtcggctgac cgccgatcag acctcgcagc cgatctcgtt ctcggcgtcg
     7261 cagtcgtcgg tgccgggacc cccgttggcg aggtcgccgt ccccatcctg gccgaggatg
     7321 aagtcgtcgc ctccctcgcc gaagagccgg tcgttcccgt caccgccgaa gaggttgtct
     7381 tcaccgggtc cgccgtggaa cacgttcacg agcgccgacc ccaccaggcc gtccggtccg
     7441 tcgccgccga tcgcgttctc gacgtccgtc ctcacgttgt cgccctcgcc ggtgatgtag
     7501 ccgtcgttgg cgacgtcatc caggacgaca tcgaccttcg ccgtgcggcc cacgtagctc
     7561 accgtgtccc ggccgggccc gccacggaac acgtcggcgc cgaaacctcc atccagccga
     7621 tcgcggccgg cgccgccg
//
LOCUS       DASUTZ010000191         2296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1747155, whole genome shotgun sequence.
ACCESSION   DASUTZ010000191 DASUTZ010000000
VERSION     DASUTZ010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1747155"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="VF097_08415"
     CDS             complement(<1..789)
                     /locus_tag="VF097_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of 2-phospho-D-glyceroyl
                     phosphate from ATP and 2-phospho-D-glycerate; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphoglycerate kinase"
                     /protein_id="HEX6262853.1"
                     /translation="MKKSKREAIVISDREPGLPYSKGLMAGQMMVTGLSPYRAYQAAE
                     RIEDLLLERNETTISNEELNALAGEVLEDLAGERYAKNFVRWQRVAQLDVPIVIVIGG
                     ATGVGKSTIATQIAARMGIVRVVPTDAVREVMRALFSRELMPTLYTSSFDAGEALRES
                     SSRQAERTVVGFREQTSAVALGVRKLIERAAMEGTSTIIEGAHVVPGFLDTSTFEDGV
                     LLVPLVVVVEDEDVHRNNFVVRSSDSAARPMGRYLENFENIRRVQ"
     gene            complement(786..2120)
                     /locus_tag="VF097_08420"
     CDS             complement(786..2120)
                     /locus_tag="VF097_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate synthetase"
                     /protein_id="HEX6262854.1"
                     /translation="MRVVALVDGEHYPPTTRWALRNAEADGHEVVAALFVGGTEKVAA
                     DGSVDLGGVPVLPGGEDAAAALGAAIEEHRPDAILDLSDEPILGYRERMALAAVALAA
                     SVPYLGPDFRLDPPIRDAPLPAPTMAVIGTGKRTGKTAIAGEAARVAAAAGLNPVVVA
                     MGRGGPPAPQVARPGTVTLERLLELVRNGMHAASDYLEDALTTGVTTVGARRAGGGLA
                     GRPYVSNVRQAAEVALREEPGLVLLEGSGAAIPPIPWDAGVLVAPASAPREYLAGYLG
                     PYRLLLSDVVVLTMAGSPDVGPEDLAYLTSLVRRLRPDARIVVTDFRPVPLDDVGGKK
                     VYFATTAPETVGPILTSYLESAHGCAVVGVTHHLSDRARLAEDIDAAPAFDVLLTELK
                     AAAVDVAADRAVRRGARVVFADNRAETLEGDGDLPDLLEEAARLAVERASGR"
BASE COUNT          320 a          837 c          814 g          325 t
ORIGIN      
        1 ctggacccgg cggatgttct cgaagttctc gaggtagcgg cccatcgggc gggccgccga
       61 gtcagacgag cgtaccacga aattgttccg atgcacgtcc tcgtcctcca cgaccacgac
      121 gagcgggacc aggaggaccc cgtcctcgaa ggtggacgtg tcgaggaagc ccgggacgac
      181 gtgggcgccc tcgatgatcg tgctcgtccc ctccatcgcg gcgcgctcga tcagcttccg
      241 gacgcccagg gcgacggccg acgtctgctc gcggaagccc accacggtcc gctcggcctg
      301 gcgggaggag gattcgcgta gggcctcccc cgcgtcgaag gacgaggtgt agagggtcgg
      361 catcagctcg cgggagaaga gggcccgcat cacctcgcgc acggcgtcgg tggggaccac
      421 gcggacgatg cccatccggg ccgcgatctg cgtggcgatc gtggactttc ccaccccggt
      481 ggcgccgccg atcacgatca cgatggggac gtcgagctga gccaccctct gccaccggac
      541 gaagttcttg gcgtagcgct cgccggcgag gtcctcgagg acctcgccgg cgagcgcgtt
      601 cagctcctcg ttcgagatcg tcgtctcgtt ccgctcgagc aggaggtcct cgatgcgctc
      661 cgcggcctgg taggcgcggt agggcgagag acccgtcacc atcatctgcc cggccatcag
      721 ccccttggag taggggaggc cgggctcccg gtcggagatg acgatcgcct cgcgcttcga
      781 cttcttcatc gcccgctcgc gcgctccacg gccagtcggg cggcctcctc caggaggtcg
      841 ggaagatcgc cgtcgccctc cagggtctcc gctctgttgt cggcgaagac cacccgggcg
      901 ccccttctca ccgcccgatc ggcggcgacg tccacggcgg ccgccttcag ctcggtgagg
      961 aggacgtcga aggcgggcgc ggcgtcgatg tcctcggcga ggcgcgctcg atcggagagg
     1021 tgatgggtga cgcccacgac ggcacagccg tgggcgctct ccagatacga ggtcaggatc
     1081 gggcccaccg tctccggagc agtggtcgcg aagtacacct tcttcccccc cacgtcgtcc
     1141 aagggaacgg gcctgaagtc cgtcacgacg atccgtgcat ccggccgcag ccgtcgcacg
     1201 agggatgtca ggtacgcgag gtcttccggc cccacgtcgg ggctcccggc cattgtaagc
     1261 accaccacgt cggagagcaa cagtcggtag ggcccgaggt agccggccag gtactcgcgg
     1321 ggcgcggagg cgggggccac gagcaccccg gcgtcccacg ggatcggggg gatcgcagcc
     1381 ccgctgccct cgaggaggac gaggcccggc tcctcgcgga gcgcgacctc agcggcctgg
     1441 cgcacgttcg agacgtaggg gcggccggcg agcccgccgc cggcccggcg cgcccccacc
     1501 gtggtcaccc cggtagtgag ggcgtcctcg aggtagtcgc tggccgcatg catcccgttc
     1561 cggaccagct cgaggaggcg ctccagggtg acggtgccgg ggcgggccac ctgcggagca
     1621 ggtggcccgc cccgccccat cgccacgacg accgggttca gcccggccgc ggcggccacg
     1681 cgcgcggcct ccccggcgat tgcggtcttc cccgtccgct tgcccgtccc gatcacggcc
     1741 atcgtcgggg cggggagcgg cgcgtcccgg atcggggggt ccagacggaa gtcgggcccc
     1801 aggtacggca cgctcgccgc gagcgccacc gcggccagcg ccatccgctc gcggtagccg
     1861 aggatcggct cgtcggagag atccaggatc gcgtccggtc gatgctcctc gatcgcggcg
     1921 ccgagcgcgg cggcggcgtc ctcgcccccg gggagcaccg ggacgcctcc caggtcgacg
     1981 ctgccgtcgg cggccacctt ctcggtgccc cccacgaaca gcgcggccac gacctcgtgc
     2041 ccgtcggcct ccgcgttgcg gagggcccac cgggtggtgg gtgggtagtg ctcaccgtcg
     2101 accagcgcga cgactctcat cagggtcgga cccgatctac tctgtcgcca gcggggcgcc
     2161 cgcgcgcggc tcctgcatca gcgcctcgag ctcccggtag tactgcgccc cggtctcgat
     2221 cgagaagtgc gcgatgacgt tcgacgcacc gaaggggaac ttccgcgaga tcttcacctc
     2281 gtccccgtcg aactcg
//
LOCUS       DASUTZ010000192        15556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_451955, whole genome shotgun sequence.
ACCESSION   DASUTZ010000192 DASUTZ010000000
VERSION     DASUTZ010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 15556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15556
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451955"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /gene="rmuC"
                     /locus_tag="VF097_08425"
     CDS             complement(<1..614)
                     /gene="rmuC"
                     /locus_tag="VF097_08425"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEX6262855.1"
                     /translation="MTGTELIVLLVALGVAGSAVLIAMRRPAPPQADARVPTMLEAQA
                     SRLDRLADALGRQTIDDQELRRSLEATKRTVEAMRTHAEARRQADEQTWEALRRLESM
                     LIGGSRRGRAGENVLEEALSSLPAQMVVRDFPVNGKRVEFALVLPDGRRLPVDSKWAA
                     AAEVEALEAEEDPAAREPIARRLEDEVAKRAREVASYLEPSLTTP"
     gene            complement(668..1597)
                     /locus_tag="VF097_08430"
     CDS             complement(668..1597)
                     /locus_tag="VF097_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6262856.1"
                     /translation="MNLLEGKVIAVTGAGRGIGREEALLLAREGARVVVNDLGGEWDG
                     TGEDTRVATEVASEIEAAGGAAAPHFEDISEPEGAASLVETALDRWGRIDGLVNNAGI
                     LRDRMTFNMAPEEWDAVIKVHLRGTFLCTAEVCRRWRELAKGGEDVSGRRIVNTSSTS
                     GLLGMVGQSNYGTAKAGIAAFTQIVSMEMRSYGVGVNAIAPGARTRMTEKTFGDLRVE
                     GDFDPLDPGNIAPLVAFLCSDSAAGITGQVFGITGGLVELYQSWTPVSELDKGARWEA
                     SGLAERMDELFGKRPRSYEPGRSPLRRITGIGG"
     gene            1714..3153
                     /locus_tag="VF097_08435"
     CDS             1714..3153
                     /locus_tag="VF097_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX6262857.1"
                     /translation="MLDIAKSAVEAARGAGAGYADARAALRETESLTVKNQEMEGVDR
                     SRTMGVGIRVLVDGFWGFASTDRREPEEIEHAAVLAVEIARAASRLPREPIALAEVEP
                     AQTTWQTVVAEDPFTIPLDEKVSLLMEAARRTQGVKGVSFAEASLDFFRQRTAFASSE
                     GAAIDQTITHSGGGIEATAISGGELQRRSFPNSFRGHFSAAGYEHIRSLGLVEEAERI
                     GEEAVALLEAPDCPSTETTLILDSSQMVLQVHESVGHPIELDRVLGMEEAYAGTSFLT
                     PDDRGKFRYASDVVTISADATVPTGIGSFGYDDEGVPAQPVPVIEEGIFQGFLSSRET
                     AAVLGETSNGTMRADGWEHLPLIRMTNVNLHPGEGTLAELIGDTKDGIFMSTNSSWSI
                     DDKRVNFQFGCEVAWRIENGRLTQLYRNPNYTGITTEFWRSCDAVCGSEEWTMWGTPN
                     CGKGQPGQVARVGHGTSPARFRNVRVGVR"
     gene            3153..4502
                     /locus_tag="VF097_08440"
     CDS             3153..4502
                     /locus_tag="VF097_08440"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX6262858.1"
                     /translation="MPEPMGREAALELAGPALDAAGGADVEVLVIRGWGGLTRFAGSA
                     IHQSTAQEETAVRVRAVRDGRVGVSSTNDLRPDGVRAAARSALEMAEMAAPDPMFPGL
                     APRAEAPTMDGYDPATSETSPAARAEAVEALVGACGPGFTAAGAFETGALEVAVANTE
                     GLAVHAARSKASITAVVSGGEGGAGFAERAAGRVADLDAAAVGAAAFAKARDSQRPRD
                     LQPGDYEVVVEPPAVATLLAFLSYLGFGGRAIAEGRSCFAGREGQRLMSERVSIWDDA
                     LSPDTIGLPFDFEGTPKRRVDLVRQGVFVGGVHDRRSGAQTGGRSTGHALPPPNPEGP
                     FPLNLFMAIGDASADDMISATGRGLLVSRFHYSNVVHPKEAVITGMTRDGTWLIEDGA
                     VVGPVKNLRFTESIIEALDRVEMVGSESTLASEFFFEASRVPTLRIGSFRFTGTSDH"
     gene            complement(4480..7272)
                     /locus_tag="VF097_08445"
     CDS             complement(4480..7272)
                     /locus_tag="VF097_08445"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6262859.1"
                     /translation="MTSTERSLLAAGTLLAGSTAVLSLMSSGPAAGTKLLPWVALAAS
                     LLVCAAWVALERRARTDAPSGRSGGREAILGAVAEAAERFLSEGVEAMPVVLEQLAGA
                     TGASRVYVWENHEGEGGALLTSQRYEWVAPGIDPQIDNPELQALPYEEAGLGRWARRL
                     GAGMTIHGPLRAFPSRERAILDPQRIRSLVAVPIFVGDEWWGFIGVDDCVRERAWSAG
                     EIDALRAAAGALGGAIHRARAMELLEQAEAKYRAIVEKIPAAIFLDALDGKATTLWVS
                     PQIEEILGFPAEEWLADPDLWMRQMHPADRDRVAAATVRHNETLEPFGEEYRIFHRDG
                     RLVWVRDEAVITYDDEGRPRFSQGFFMDVTEGKRAEAQERRHSEYLSAIHETALGLMD
                     RLEVDGLLQAIVGRAAELLGTGHGFVYLPDETGERMEMKVGVGALTEAVGLQAGRGEG
                     AAGLVWERGEPVVISDYAGWEGRLSFSLDVSSVVAVPLGSRNEVVGVLGLAHMDPELG
                     FTDEDVEHLVTFAEFASIALDNARLYEAAQRELAERKRAQAELEEQEERFRTLSDSAL
                     EGICIHDRGTITEVNRAFAEMFRMPPEELVGTQVLELLAPEDREMAARRIEEQSEEVG
                     RHEGVRGDGSRFAVELVGRRIPYRGREARVLAIRDITERRRAEESLREAYEREREAAE
                     QLRHVDEMKNAFLTAVSHELRTPLSAILGFALTLERESLSLSPAEVREMVDRIVANAR
                     KLDSLLTDLLDLDRISRGILEATRRPTDVGDLVHRVVEGADLAGRRVLVEAQELVAQV
                     DGPKVERIVENLLANAAKYTPPGTPVWVRLVRRPGGVLIAVEDAGPGVELEQRDAIFR
                     PFTQGEDAPAHAPGTGIGLSLVARFAEMHGGSAWVEDREGGGASFRVFLAAAEVESSE
                     LPRAVGFGELSDRSSQ"
     gene            7394..8326
                     /locus_tag="VF097_08450"
     CDS             7394..8326
                     /locus_tag="VF097_08450"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262860.1"
                     /translation="MALASILIAAGSLLLYGGAELADRGLSRVSIPGVPALGSRTIRV
                     GAEVGVLAAALAAAGRGETEIAGGLATGGALFLLAVGMGWRLAVGRRPLFAPSSTLLL
                     LAPSGAVILAALAVSGRYVDRLEGLLLAVGFAGYLVLQLREQAVLSGGDEPPPKRPSE
                     PEREPDGARSAAPAVAGIGLLAGGAVVIVEGGVRLSLAGGLGAGFVGASLLAALTTLH
                     RVIPRSLGGEPPERAAGSLLGAITALLTGVLGLAALVRPLTIDTGVNLAFLGVAVVYT
                     VVAVALLLRRRAGWILGIALLASYASWLVYASGA"
     gene            complement(8323..9441)
                     /locus_tag="VF097_08455"
     CDS             complement(8323..9441)
                     /locus_tag="VF097_08455"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6262861.1"
                     /translation="MRPLRGIRVADLSRVLAGPLCTMVLADLGAEVVKVERPGVGDET
                     RSWGPPFVGQDAAYFLSVNRGKRSLELDLKAPEGAAAAKELILRSDVLVENFRPGTMA
                     RLGLDPEELGERHPGLVVCSITAMGEEADPRVAGYDLVMQAATGLMSLTGPAGGEPTK
                     VGVALLDVVAGLYAAVGITAALRDRERTGRGTRVSVSLFDAGVAALVNQAANYLLGGE
                     VPVPMGNAHPNIAPYEALSTPGGLLVLAAANDGLFVRACEVLGRPDLPSDARFARNAD
                     RVRNREALRGLLEEALAERPRDEWIGLLIEAGVPAAPVRDLAAVFGSDEGRATIQEID
                     DPVRGLLRLVRSPIRLDGVAESASRPPPALGTHEPDDP"
     gene            complement(9438..11018)
                     /locus_tag="VF097_08460"
     CDS             complement(9438..11018)
                     /locus_tag="VF097_08460"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6262862.1"
                     /translation="MDEVPAGRYLESFDPATGELVGRLPRTPPEEVEAVAAAVAEVQP
                     TWARAPLSDRLDVIGRAADRMLDRREELSLLFTRESGKPITESSIAEVGASALVLSWV
                     ARHGRRHLSPERVPTSQPIVAHKKHWVVFDPLGVIGVIGPWNYPCLLPMADVAFALAA
                     GNGVVFKPSELTPLSGQAIADLFAEAGLPEGVLRVVHGEGDTGAALCEAGPVQKILFT
                     GSVESGRKVLEETARAGKMAQLELGGNDPAVVCADADLERAVAGVLWASCANAGQTCA
                     AIERVYVDRRIHDRFVAGLVAAAETLVPGDPKRPDTQIGPIISERQHRRVLEHLEDAR
                     RRGATVECGGPVEVPGLAGLFVAPAVLTGVDHSMRVMREETFGPLIPVMAFDDEREAL
                     ALANDSEFGLGASVWTRDARRGRALALGIEAGMVWINDHAYSNGLAEAPWGGVKSSGT
                     GVAHSRHGLQDMTQKRLIAEDAGRVPAGWWFPYSEIRRRGFLAVVESLARARRARRVR
                     AAWDGRGTIGRYVRSLLG"
     gene            complement(11046..11876)
                     /locus_tag="VF097_08465"
     CDS             complement(11046..11876)
                     /locus_tag="VF097_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018544349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX6262863.1"
                     /translation="MSIDLHTHTRHSDGTFEPRESVGLALDRGLRVLAVTDHDTTAGV
                     SAAEEAAAGTGLEIVPGVEFSAEYEGGSVHVLGYWMDPEHEEFRAELARLQDDRVRRG
                     ERMVERLQALGYPVTVERVREIAAGGSVGRPHVAQALVEAGVVPTIEDAFTADLIATG
                     GRAYVQKHALHPVEAVRLIRRANGLAVLAHPVHWRDGIPVPHTLIQEMIEAGMDGIEC
                     AHPDQDRATEARYREMSARHGLVATGSSDCHGTRYDPIRMGSRTTDREEFEKLLARRR
                     "
     gene            11968..12171
                     /locus_tag="VF097_08470"
     CDS             11968..12171
                     /locus_tag="VF097_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262864.1"
                     /translation="MATTAERLREPDMLRSYLHALARNGGTGREELGHGRQRRVCTHC
                     GERAVFTPDTDGWYRCSHCGRYA"
     gene            complement(12172..14301)
                     /locus_tag="VF097_08475"
     CDS             complement(12172..14301)
                     /locus_tag="VF097_08475"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEX6262865.1"
                     /translation="MARPPATVDIREVQVIKHDRTFFVSNRFGDVPEGNTAALGLYQA
                     DTRFLSRLEVTLNQLRPLLLHSSTERNYSQIVELVYPVRHVLLAGPERKDNISLQRRR
                     VLHGSLFERMRIRNFGLQDRRIGLTVSFESDFLDIFEVRGMAREERGEMLPPEVDRHS
                     VVLGYRGLDGVTRSTTLRFSPAPETLSEREASFDLEVAAGDQVDIVLEIEPHVGDAAP
                     TRRSMREIEQELEQVYAAWEKRSTRFGASDVRLQGFLRRAVRDLRMLSSTGENGTRFI
                     DAGIPWYSAMFGRDSLITAYQALTGCPKLAWSTLRGLAALQGKVDDPWREEEPGKILH
                     ELRVGELAGADEIPHTPYYGSIDATPLWLVVLGAAFRWTGDREALRELWPNALAALRW
                     IDEHGDRDGDGYVEYERRSPRGLDNQGWKDSWDGVPWPDGTPVEPPIALVEVQGYVYQ
                     AKSLMAQLAREMGEEGLAARLESEAAELKERFNRDFWEEETGFFALALDGRKRRVPTI
                     TSNPGHCLWSRIVDRRLAPQVVKRLMDPGLLSGWGIRTLSSRHPVYDPIGYHTGTIWP
                     HDNSLIAQGLKRYGFDAEAQDVLDQLTAAGRFFPYSRFPEVFCGFSSDEVPVPVEYPV
                     ACRPHAWASAAPLLMVRAYGGLSADSARRRLTIERPRLPDWLTGIELRGMHVAGARLD
                     LSFVNSDGVTAVKVDHKRGDLEVVIRQ"
     gene            14372..14605
                     /locus_tag="VF097_08480"
     CDS             14372..14605
                     /locus_tag="VF097_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262866.1"
                     /translation="MLGADGFYDAGEIGCGGPTLKEIGRLVDELPAGSTVEVRSATHT
                     GRNSLRAFCRLRELAIVAEDAGPDRDRILIRKT"
     gene            14616..15014
                     /locus_tag="VF097_08485"
     CDS             14616..15014
                     /locus_tag="VF097_08485"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEX6262867.1"
                     /translation="MTIVIHASHGAEDPERATLPFVLGNNAAVAGQDPVILLIVEAVH
                     LATEGGADGVQKQGLPPLPEIMAEYVSNGGAIWACRSCTKPRGIEEGDLSREPGSRRR
                     WRSWSSSREARPASPSDLRRPPAMLGPWPW"
     gene            15002..>15556
                     /locus_tag="VF097_08490"
     CDS             15002..>15556
                     /locus_tag="VF097_08490"
                     /inference="COORDINATES: protein motif:HMM:NF013383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HEX6262868.1"
                     /translation="MALVTVFGAGAAGTALAIHLARKGEDAVLWGSEHDAAVLGDLTE
                     KRRHPALPERLPDELRILGPDDLEEAGAHAEIAVLGANSAGAGPLGRLVAPALGGARL
                     AVSIAKGLQPETLERVSAVYARELPSIAVVAMSGPSLAAEIAEGLLTAVAFACEDEGA
                     CKEAAEAFRSDRFLVSTTDDVVGVE"
BASE COUNT         2169 a         5752 c         5433 g         2202 t
ORIGIN      
        1 ggggtggtca gcgacggctc gaggtacgac gcgacctcgc gcgcccgctt cgccacctcg
       61 tcctcgagcc gacgggcgat gggctcgcgc gcggcggggt cctcctcggc ctccagggcc
      121 tcgacctccg cggcggcggc ccacttcgag tccacgggga gacgccgccc gtccggcagg
      181 acgagcgcga actccacacg cttcccgttc acggggaagt cgcggacgac catctgagcc
      241 ggcagggacg agagcgcctc ctcgaggacg ttctccccgg cccggccccg cctggagccg
      301 ccgatcagca tcgactcgag ccgtcggagc gcctcccacg tctgctcgtc tgcctgccga
      361 cgggcctcgg cgtgcgtccg catcgcctcg accgtgcgct tcgtggcctc gagcgagcgg
      421 cgaagctcct ggtcgtcgat cgtctgacga cccagcgcgt cggccagccg gtccaggcgg
      481 ctcgcctgcg cctcgagcat cgtgggcacg cgcgcgtccg cctggggcgg cgccggccga
      541 cgcatggcga tcagcacggc cgaccccgcc acccccaggg cgaccagcag cacgatcagc
      601 tcggttccgg tcatcgcgac ctcctctcag ggccactgta gagagggggt ctgacacgaa
      661 cggcggctca gccgccgatg cccgtgatgc gccgcagggg cgagcggccg ggctcgtagg
      721 agcgcggccg cttgccgaag agctcgtcca tccgctcggc gagcccggac gcctcccacc
      781 gggccccctt gtcgagctcc gacacgggcg tccagctctg gtagagctcg acgagccccc
      841 cggtgatccc gaacacctgg ccggtgatcc cggcggcgga gtccgagcag aggaacgcca
      901 cgagcggcgc gatgttgccc gggtcgagcg gatcgaagtc gccctccacc cggaggtcac
      961 cgaaggtctt ctcggtcatg cgggtccggg cgccgggcgc gatcgcgttc acgcccacgc
     1021 catagctccg catctccatc gagacgatct gggtgaacgc ggcgatgccg gccttggccg
     1081 tcccgtagtt cgactgcccc accatcccca ggagtcccga ggtgctcgag gtgttcacga
     1141 tgcggcggcc cgagacgtcc tcgcctccct tcgccagctc gcgccagcgg cggcagacct
     1201 cggccgtgca caggaaggtt ccccggaggt ggaccttgat caccgcgtcc cactcctcag
     1261 gagccatgtt gaaggtcatg cggtcgcgca ggatccccgc gttgttgacg aggccgtcga
     1321 tgcggcccca gcgatcgagc gcggtctcga ccagggatgc ggccccctcg ggctcggaga
     1381 tgtcctcgaa atggggcgcg gccgcacccc ccgcggcctc gatctcgctc gccacctcgg
     1441 tggccacgcg ggtgtcctcc ccggtgccgt cccactcgcc gccgaggtcg ttgacgacga
     1501 ccctcgcgcc ctcgcgggcc agcagcagcg cctcctctcg gccgatcccc cggccggcgc
     1561 cggtgacggc gatcaccttc ccttcgagca agttcatgca tcgctccttt tccgatccgc
     1621 ccacacgata gacgagcctc tgggtcgagg acggcctcgt cggatacact gcgctccgtc
     1681 cgtccagagg cgggttcgtc cgaaaggaga ggcatgctcg acatcgcgaa gtcggcggtc
     1741 gaggcggcgc gcggcgcggg cgccggctac gccgacgccc gcgccgcgct ccgcgagacc
     1801 gagtcgctca ccgtcaagaa ccaggagatg gagggcgtgg accgctcccg caccatgggg
     1861 gtggggatcc gggtcctcgt ggacggcttc tggggcttcg cctccaccga ccgcagggag
     1921 cccgaggaga tcgagcacgc cgcggtcctg gccgtggaga tcgcccgcgc cgcgtcccgc
     1981 cttccccgcg agccgatcgc cctggccgag gtggagcccg ctcagacgac gtggcagacg
     2041 gtcgtggccg aggacccgtt cacgatcccc ctcgatgaga aggtctcgct cctcatggag
     2101 gccgcgcgcc ggacccaggg cgtcaagggg gtgtcgttcg ccgaagccag cctggatttc
     2161 ttccgtcagc ggacggcgtt tgcctcgtcc gagggggcgg cgatcgacca gacgatcacg
     2221 cactcgggcg gcgggatcga ggccacggcg atctccggcg gcgagctcca gcgccgctcg
     2281 ttccccaact cgttccgggg ccacttctcg gcggccggct acgagcacat ccggtccctg
     2341 gggctcgtgg aggaggccga gcggatcggc gaggaggcgg tggccctcct cgaggccccc
     2401 gactgcccct cgaccgagac cactctgatc ctcgactcct cgcagatggt gctccaggtc
     2461 cacgagtcgg tgggccaccc gatcgagctc gaccgcgtgc tcgggatgga ggaggcctac
     2521 gcgggcacat cgttcctcac cccggacgac cggggcaagt tccggtacgc ctccgacgtc
     2581 gtgacgatct cggccgacgc caccgtcccc acggggatcg ggtcgttcgg ctacgacgac
     2641 gagggcgtgc ccgcccaacc cgtgccggtc atcgaggagg gcatcttcca gggcttcctc
     2701 tccagccggg agacggccgc cgtcctcggg gagacgtcca acggcacgat gcgcgcggac
     2761 ggctgggagc accttccgct gatcaggatg acgaacgtca acctccaccc gggcgaaggg
     2821 acgctggccg agctgatcgg cgacacgaag gatggcatct tcatgtccac gaacagctcg
     2881 tggtcgatcg acgacaagcg cgtgaacttc cagttcggct gcgaggtggc atggcggatc
     2941 gagaacggcc gcctcaccca gctctaccgc aaccccaact acacgggcat cacgaccgag
     3001 ttctggcgct cctgcgacgc ggtctgcggg agcgaggagt ggacgatgtg gggcacgccg
     3061 aactgcggga agggtcagcc ggggcaagtg gcccgggtgg ggcatgggac ctcgccggcc
     3121 cgattccgga acgtcagggt gggggtgcgc tgatgcccga gccgatgggg cgagaggcgg
     3181 cgctggagct cgccgggccg gccctcgacg cggccggcgg cgccgacgtc gaggtcctcg
     3241 tgatccgtgg ctggggaggc ctgacgcggt tcgccggatc ggcgatccac cagagcaccg
     3301 cccaggagga gaccgcggtc cgcgtgcggg cggtccgcga cggacgggtg ggagtctcct
     3361 cgacgaacga cctccggccc gacggcgtcc gggccgcggc gcgaagcgcg ctggagatgg
     3421 ccgagatggc cgcgcccgac ccgatgttcc ccggactcgc ccctcgggcc gaggctccga
     3481 cgatggacgg ctacgacccc gccacgtccg agacgtcgcc cgcggccagg gccgaggccg
     3541 tcgaggcgct cgtgggggcc tgcggccccg gcttcacggc cgcgggtgcg ttcgagacgg
     3601 gcgccctcga ggtggccgtg gccaacacgg aggggctggc cgtccacgcc gcacggagca
     3661 aggcctcgat cacggcggtc gtctccggcg gcgagggagg cgcggggttc gccgagcgag
     3721 ccgccggacg agtggccgac ctggacgccg cggcggtggg cgcggcggcc ttcgccaagg
     3781 cccgcgacag ccagcggccc cgtgacctcc agccggggga ctacgaggtg gtggtggagc
     3841 cccccgccgt ggccacgctg ctcgccttcc tctcctatct cgggttcggg gggcgggcca
     3901 tcgccgaggg ccgctcgtgc ttcgccggca gggagggcca gcggctgatg tccgagcggg
     3961 tctcgatctg ggacgacgcg ctgtccccgg acacgatcgg gctcccgttc gacttcgagg
     4021 ggacgcccaa gcgacgagtg gacctcgtgc gccagggggt cttcgtcggg ggcgtccacg
     4081 atcgcaggag cggagcgcag accgggggcc ggtccaccgg ccatgcgctc ccgcctccca
     4141 accccgaggg accgttcccc ctcaacctgt tcatggcgat cggcgacgcg tcggccgacg
     4201 acatgatctc agccacgggc cggggcctcc tcgtcagccg cttccactac tcgaacgtgg
     4261 tgcaccccaa ggaggccgtg atcaccggga tgacccggga cggcacctgg ctgatcgagg
     4321 acggagccgt cgtgggtccc gtgaagaacc tccgcttcac cgagtcgatc atcgaggccc
     4381 tcgaccgcgt cgagatggtc ggctccgagt ccacgctcgc cagcgagttc ttcttcgagg
     4441 cctcacgcgt cccgacgctc cggatcggct cgttccggtt cactgggacg agcgatcact
     4501 gagctccccg aacccgacgg cccgcggcag ctccgaggac tccacctcgg ccgcggcgag
     4561 gaacacccgg aacgacgccc cgcctccctc tcgatcctcg acccaggcgc tcccgccgtg
     4621 catctcggcg aaccgagcca cgagcgagag gccgatcccc gtcccggggg cgtgggccgg
     4681 ggcgtcctcg ccctgggtga acggccggaa gatcgcatcc cgctgctcca gctccacgcc
     4741 cggccccgcg tcctccacgg cgatcagcac cccgccgggc cttcggacca ggcgcaccca
     4801 gaccggggtc ccgggaggcg tgtacttcgc cgcgttcgcc agcagattct ccacgatccg
     4861 ctccaccttc ggcccgtcca cctgcgcgac gagctcctgg gcctccacga gcacgcgtcg
     4921 gccggcgaga tcggctccct ccaccacgcg gtgaaccagg tcgccgacat ccgtcggtcg
     4981 ccgcgtcgcc tcgaggatcc cgcgcgagat gcgatcgagg tcgaggaggt ccgtcagcag
     5041 agagtcgagc ttcctggcgt tggccacgat ccgatcgacc atctcgcgca cctcggccgg
     5101 tgagaggctg aggctctccc gctccagcgt cagcgcgaac cccaggatgg cggagagcgg
     5161 ggttcggagc tcgtggctca ccgccgtgag gaaggcgttc ttcatctcgt cgacgtgtcg
     5221 gagctgctct gccgcctcac gctcccgctc gtacgcttcg cgcagggact cctcggcccg
     5281 ccgccgctcg gtgatgtccc ggatcgccag gacccgggcc tccctccccc ggtaagggat
     5341 cctgcgtccc acgagctcca ctgcgaagcg ggacccgtcg ccccggaccc cctcgtgccg
     5401 cccgacctcc tcggactgct cctcgatccg cctggcggcc atctcgcgat cctcgggcgc
     5461 caggagctcc aggacctggg tcccgacgag ctcctcgggt ggcatccgga acatctcggc
     5521 gaaggcccgg ttgacctcgg tgatcgttcc ccggtcgtgg atgcagatcc cctccagggc
     5581 cgaatcggac agcgtgcgga agcgctcctc ctgctcctcc agctcggcct gggcccgctt
     5641 ccgctcggcg agctcgcgct gggcggcctc gtatagccgc gcgttgtcca gggcgatcga
     5701 ggcgaactcg gcgaaggtca ccaggtgctc cacgtcctca tcggtgaagc cgagctccgg
     5761 gtccatgtga gcgaggccca ggacccccac cacctcgttc cgggatccga gggggaccgc
     5821 cacaacgctc gacacatcga gagagaagct cagacggccc tcccagcccg cgtagtctga
     5881 gatcacgacc ggctcccccc gctcccaaac cagcccggcc gccccctccc cgcgcccggc
     5941 ctgcaacccc accgcttcgg tgagggcccc cacgccgacc ttcatctcca tacgctcgcc
     6001 ggtctcgtcg gggaggtaga cgaacccgtg acccgtgccg agcagctccg ccgcccgccc
     6061 cacgatcgcc tgcaggaggc cgtcgacctc gaggcgatcc atgagaccga gcgcggtctc
     6121 atggatcgcc gagaggtact ccgagtggcg ccgctcctgc gcctccgcgc gcttcccctc
     6181 ggtcacgtcc atgaagaagc cctgcgagaa gcgcggcctg ccctcgtcgt cgtaggtgat
     6241 cacggcctca tcccggaccc acaccaatcg gccgtcgcgg tgaaagatcc ggtactcctc
     6301 cccgaagggc tccagggtct cgttgtggcg gacggtggcg gcggcgacgc ggtcgcggtc
     6361 ggccgggtgc atctggcgca tccacaagtc tgggtcggcg agccactcct cggcggggaa
     6421 ccctaggatc tcctcgatct gagggctcac ccagagcgtg gtggccttcc cgtccagggc
     6481 gtccaagaag atggcggcgg ggatcttctc cacgatcgcc cggtacttcg cctcggcctg
     6541 ctccagcagc tccatcgctc tcgctcgatg gatcgccccg ccgagagcgc ccgccgcggc
     6601 gcgcagggcg tcgatctccc cggcggacca tgcgcgctcc ctcacgcagt cgtccacgcc
     6661 gatgaagccc caccattcgt cgcccacgaa gatggggacg gcgacgagcg agcggatccg
     6721 ctgaggatcg aggatcgccc gctcgcgaga ggggaacgcc cggaggggcc cgtggatcgt
     6781 catcccggcg cccaggcgac gggcccagcg acccaggccc gcctcctcgt acggcagggc
     6841 ctgcagctcg gggttgtcga tctgcgggtc gatgcctggg gccacccact cgtagcgctg
     6901 cgaggtgagc agcgcgccgc cttcgccctc gtggttctcc cacacgtaga cgcggctggc
     6961 ccccgtcgcc cccgcgagct gctcgaggac cacgggcatc gcctccacgc cctcgctgag
     7021 gaagcgctcc gcggcctccg ccacggctcc gaggatcgcc tcacgccctc ccgagcgtcc
     7081 ggacggggcg tcggtgcggg cccggcgctc gagagcgacc caggccgcgc agacgaggag
     7141 tgatgcagcg agggccaccc acggcagcag tttcgtcccc gctgccggac ccgaggacat
     7201 gagcgagagc acggcggtgg agccggcgag cagcgtaccg gcggcgagga ggctccgctc
     7261 ggtcgaggtc atctctcaat gaggttgtcg gaagcgatgg cggcgtccct ccatggtcat
     7321 ttcgagccat cttccgggcc gccggatggg cgacgtggcc cgggcggacc ggctcatcct
     7381 ctatcgtgcg cgcatggccc tcgcctcaat cctgatcgcg gccggctccc tgctcctcta
     7441 cggaggggcc gagctcgccg accggggcct gtcccgcgtc tcgatccccg gcgtcccggc
     7501 actcgggtcc cggacgatcc gggtcggggc cgaggtcggc gtcctcgcgg cggcgctggc
     7561 ggctgccgga cgcggcgaga cagagatcgc gggggggctg gccaccggcg gggcgctgtt
     7621 cctgctcgcc gtggggatgg ggtggaggct cgccgtcggc cggcggcccc tcttcgcgcc
     7681 gtcctctacc cttctgctgc tggccccgtc cggagccgtg atcctggccg cgctcgccgt
     7741 gagcggtcgc tacgtggacc gcctggaggg gcttctgctg gccgtggggt tcgccgggta
     7801 cctcgtcctc cagctgaggg agcaggcggt cttgtcgggg ggcgacgagc ccccaccgaa
     7861 gcgtccctcg gagccggagc gcgagccgga tggggcgcga tcggccgctc cggcggtcgc
     7921 ggggatcggc ctcctggccg gcggcgccgt cgtgatcgtg gagggaggag tccggctctc
     7981 gctggccggg ggcctggggg cgggcttcgt gggagcctct ctgctggcgg ccctcacgac
     8041 gctccaccgc gtgatcccgc gatccctcgg cggggagcct ccggaacgcg ccgccggaag
     8101 cctcctcggg gcgatcacgg ccctgctcac cggcgtcctg ggactggccg ccttggtccg
     8161 gccgctcacg atcgatacgg gcgtcaacct cgccttcctg ggggtggccg tggtctacac
     8221 ggtcgtggcc gtcgccctgc tgctgcggcg tcgggccggg tggatcctcg ggatcgccct
     8281 cctcgcttcc tacgcgagct ggctcgtcta cgcgtccgga gcctaggggt cgtccggctc
     8341 gtgtgtcccc agcgcgggcg gcgggcgtga cgccgactcg gcgacgccgt cgagccggat
     8401 cgggctccgc acgagccgca ggaggccgcg caccggatcg tcgatctcct ggatcgttgc
     8461 ccggccttcg tccgatccga agaccgcggc gaggtcgcgc accggggccg cgggaacccc
     8521 ggcctcgatc agcaggccga tccactcgtc cctcgggcgc tccgcgagcg cctcctccag
     8581 gaggccgcga agggcctctc gattccgcac gcgatcggcg ttgcgggcga acctcgcgtc
     8641 cgaggggagg tcgggccgtc ccagcacctc gcatgcgcgg acgaagaggc cgtcgttcgc
     8701 cgcggcgagc acgagcagcc cccctggggt cgagagcgcc tcgtagggag cgatgttggg
     8761 gtgcgcgttg cccatcggga cgggcacctc tccccccagc aggtagttgg cggcctggtt
     8821 gacgagcgct gccaccccgg cgtcgaacag cgagacggac acgcgcgtcc cccgccccgt
     8881 ccgctcgcgg tcgcggagcg cggcggtgat ccccacggca gcgtagaggc cggcgaccac
     8941 gtcgaggagc gcgacgccga ccttcgtggg ctccccgccg gccggacccg tcagactcat
     9001 gagcccggtc gccgcctgca tcacgaggtc gtagccggcc acccggggat cggcctcctc
     9061 gcccatggcc gtgatcgagc acacgacgag gcccggatgg cgctcgccca gctcctcggg
     9121 atcgagaccc aggcgagcca tcgtgcccgg gcggaagttc tccacgagga cgtccgagcg
     9181 gaggatcagc tccttggccg ccgcggcgcc ctcgggcgcc ttgaggtcga gctcgagcga
     9241 gcgcttcccc cggttcaccg agaggaagta cgcggcgtcc tgcccgacga acggcggccc
     9301 ccaggagcgg gtctcatcgc ccacccccgg ccgctcgacc ttcaccacct ccgcccccag
     9361 atcggccagg accatcgtgc agagcggtcc cgccaggacg cgggacaggt cggcgacccg
     9421 gatgcctcga agcggcctca cccgagcagg gaccgcacgt aacgcccgat cgtgccccgc
     9481 ccgtcccacg ccgcgcgcac ccgccgcgca cgccgggccc gggccagcga ctcaacgacg
     9541 gccaggaagc ctcgtcttcg gatctcgctg tacgggaacc accaaccggc cgggaccctc
     9601 ccggcatcct cggcgatcag ccgcttctgg gtcatgtcct ggaggccgtg cctggagtgc
     9661 gccaccccgg tccccgacga cttcacaccg ccccagggcg cctccgccag cccgttcgag
     9721 tacgcgtggt cgttgatcca gaccatgccg gcctcgatcc ccagggccag ggcgcggccc
     9781 ctccgcgcgt cgcgggtcca cacgctcgcc ccgagcccga actcggagtc gttggccagc
     9841 gccagtgcct ctcgctcgtc gtcgaacgcc atcaccggga tcagcggccc gaacgtctcc
     9901 tcgcgcatga cccgcatcga gtgatccacc ccggtcagaa cggcgggggc cacgaagagc
     9961 cccgcgagcc ccggcacctc taccggccct ccgcactcga cggtcgcgcc ccggcggcgg
    10021 gcgtcctcca ggtgctccag gacccgccgg tgctgacgct cggagatgat cggccctatc
    10081 tgagtgtccg ggcgcttcgg gtcgccggga acgagtgtct cggccgccgc aacgaggccg
    10141 gccacgaacc ggtcgtggat gcgcctatcg acgtagaccc gctcgatcgc agcacaggtc
    10201 tgcccggcgt tcgcgcacga ggcccagagc accccggcca cggcccgctc gaggtccgcg
    10261 tccgcgcaga cgaccgcggg gtcgttccct ccgagctcca gctgggccat ctttccagcc
    10321 cgggccgtct cctcgaggac cttccgcccg ctctccacgc tccccgtgaa gaggatcttc
    10381 tggaccggcc ccgcctcgca cagcgcggcc ccggtgtcgc cctccccgtg cacgacgcgc
    10441 agcacgccct cgggcagccc ggcctccgcg aacaggtcgg cgatcgcctg gcccgagagc
    10501 ggggtgagct cggacggctt gaagaccacc ccgttgcccg cggccaaggc gaaggccacg
    10561 tccgccatcg gaagcagaca cgggtagttc cacggcccga tgacgccgat cacgcccagg
    10621 ggatcgaaga cgacccagtg cttcttgtgg gccacgatcg gctgcgaggt ggggacgcgc
    10681 tcgggcgaga gatggcgacg cccgtgtcgg gcgacccagg agaggacgag cgccgaggcg
    10741 cccacctccg cgatcgagga ctcggtgatg ggcttccccg actccctggt gaagagaagg
    10801 ctgagctcct cccgccggtc gagcatccgg tcggcggccc ggccgatcac gtccaggcga
    10861 tcgctcaggg gcgcgcgggc ccacgtcggc tggacctcgg ccacggcggc cgccacggcc
    10921 tcgacctcct ccggcggcgt gcgtggaagc cgccccacga gctcgcccgt ggcagggtcg
    10981 aagctctcca gatacctccc ggccgggacc tcatccacgg cccgagccat catcctcctc
    11041 ccgactcagc ggcggcgggc cagcagcttc tcgaactcct cgcgatcggt ggtccgcgat
    11101 cccatgcgga tgggatcgta gcgggttccg tggcagtccg aggaccccgt ggccacgagc
    11161 ccgtgacggg ccgacatctc ccggtagcga gcctcggtgg cacggtcctg gtcggggtgg
    11221 gcgcactcga tgccgtccat ccccgcctcg atcatctcct ggatgagcgt gtgcgggacg
    11281 gggatgccgt cgcgccagtg caccgggtgc gcgaggacgg cgaggccgtt cgctcgccgg
    11341 atcaagcgga ccgcctccac gggatggagg gcgtgcttct gcacgtaggc gcgcccgccc
    11401 gtcgcgatca ggtccgcggt gaacgcgtcc tcgatcgtgg gcaccacgcc cgcctccacc
    11461 agcgcctggg ccacgtgggg acggcccacg cttcccccgg ccgcgatctc gcggacccgc
    11521 tcgacggtga ccggatagcc cagcgcctgg agacgctcca ccatccgctc gccgcgccgg
    11581 acccgatcgt cctgcagcct ggctagctcg gcgcggaact cctcgtgctc gggatccatc
    11641 cagtacccga ggacgtggac gctccccccc tcgtactcgg ccgagaactc gacgcccggg
    11701 acgatctcga gccccgtgcc ggccgccgcc tcctcggccg cggagacccc cgccgtggtg
    11761 tcgtggtcgg tcaccgcgag cacgcggagc ccgcgatcga gggccaggcc caccgactca
    11821 cggggctcga acgtcccgtc cgaatgacgc gtatgcgtgt gcagatcgat gctcatcgct
    11881 cgaccagtgt agtggcccgt tctccgcgtt catcctccgc gggagtgggt acaacccgtc
    11941 gggaccggga aggaagaggt gagcggcatg gcaaccactg cagagcgtct cagagagccc
    12001 gacatgcttc ggtcgtacct ccacgcgctc gcgcggaacg gcggcaccgg ccgcgaggag
    12061 ctggggcacg gccgccagcg gcgggtctgc actcactgcg gggagcgggc ggtgttcacg
    12121 cccgacaccg acggctggta ccgttgctcg cactgcgggc gctacgcctg atcactgccg
    12181 gatcacgacc tccaggtcgc cccgcttgtg atcgaccttc acggccgtga cgccgtcgct
    12241 gttcacgaag gagagatcaa ggcgggcccc ggccacgtgc atccctcgca gctcgatccc
    12301 ggtcagccag tccggcagcc ggggacgctc gatggtcaga cgccgtcgcg ccgagtcggc
    12361 ggacagccct ccgtaggccc ggaccatgag cagcggggca gcggacgccc acgcgtgggg
    12421 ccggcaggcg accgggtact cgacggggac cgggacctcg tcgctggaga atccgcagaa
    12481 cacttccggg aacctcgagt aggggaagaa tcgaccggcc gccgtgagct ggtccaggac
    12541 gtcctgggcc tccgcgtcga acccatacct cttgagtccc tgagcgatga gtgagttgtc
    12601 gtgaggccag atcgtgcccg tgtgataccc gatcgggtcg tagacggggt gtcgcgagga
    12661 gagcgtcctg atcccccatc ccgagagcag gccgggatcc atcaggcgct tcaccacttg
    12721 gggggcgagg cgccggtcca cgatccgcga ccagagacag tggccgggat tcgacgtgat
    12781 ggtcggcact cggcgcttcc ttccgtcgag cgcgagcgcg aagaatcccg tctcctcctc
    12841 ccagaagtcg cggttgaagc gctccttgag ctcggcggcc tcgctctcga gccgcgccgc
    12901 gaggccctcc tcgcccatct cccgcgccag ttgcgccatc aggctcttgg cttggtacac
    12961 gtatccctgg acctcgacca gggcgatggg cggctccacc ggtgtgccgt ccggccaggg
    13021 gacgccgtcc cacgaatcct tccatccctg gttgtcgaga cccctgggag agcgccgctc
    13081 gtactccacg tagccgtcgc cgtcacgatc cccgtgctcg tcgatccagc ggagtgcggc
    13141 gagcgcgttc ggccagagct ctcggagcgc ctcccgatcc cccgtccacc ggaacgccgc
    13201 ccccaggacg acgagccaga ggggcgtcgc gtcgatcgac ccgtagtagg gggtgtgggg
    13261 gatctcgtcg gccccggcga gctcaccgac gcgcagctcg tggaggatct tccccggctc
    13321 ctcctcccgc cagggatcgt cgaccttccc ctggagcgcc gcgagccccc ggagcgtgct
    13381 ccacgccagc ttggggcagc ccgtgagcgc ctggtaggcc gtgatgaggg agtctcggcc
    13441 gaacatcgcc gagtaccacg ggatcccggc gtcgatgaag cgggtgccgt tctcaccggt
    13501 cgagctgagc atgcggaggt cgcgcacggc ccgcctcagg aagccctgga ggcgcacgtc
    13561 gctggcaccg aagcgggtgg agcgcttctc ccaggcggcg tacacctgct ccagctcctg
    13621 ctcgatctcg cgcatcgatc ggcgggtcgg ggcggcgtcg cccacgtggg gctcgatctc
    13681 caggacgatg tcgacctggt cgccggccgc cacctcgagg tcgaacgagg cctcgcgctc
    13741 gctcagcgtc tcgggggcgg gcgagaaacg gagggtcgtc gaccgggtga ccccgtccag
    13801 acctcgatag ccgaggacga cgctgtgacg gtccacctcc gggggcagca tctcgcctcg
    13861 ctcctcccgg gccatccccc gcacctcgaa gatgtcgagg aagtcggact cgaaggagac
    13921 ggtcagcccg atgcggcgat cctggaggcc gaagttccgg atccgcatcc gctcgaagag
    13981 gctcccgtga aggacccgcc tgcgctggag cgagatgttg tccttccgct cgggacccgc
    14041 cagcaggacg tgccgcaccg ggtagacgag ctccacgatc tgcgaatagt tccgctcggt
    14101 cgaggagtgc agcagcagcg gccgcagctg attgagcgtt acctcgagcc gcgagaggaa
    14161 cctcgtgtcg gcctgataga ggccgagcgc cgcggtgttg ccctccggga catcgccgaa
    14221 gcggttcgag acgaagaagg tccggtcgtg cttgatgacc tggacctccc ggatgtccac
    14281 ggtcgccggc ggcctggcca cgagcccatc ctaagccggg ccgtccccgg tcggggagaa
    14341 tggcggcggg aggaggagcg gatggggatc ggtgctgggg gccgacggtt tctatgacgc
    14401 gggcgagatc gggtgcggcg gccccacgct gaaggagatc gggcggctgg tggacgagct
    14461 gccggcgggc agcaccgtcg aggtgcgctc agccacgcac accgggagga acagcctgag
    14521 ggccttctgc cggctccggg agctggcgat cgtggccgag gacgcgggcc cggaccgcga
    14581 ccgcatcctg atccgcaaga cctgaggagg accgcatgac gatcgtgatc cacgcgagcc
    14641 acggagcgga ggacccggag cgggcgaccc tgcccttcgt gctcgggaac aacgcggcgg
    14701 tggccgggca ggatcccgtg atcctgctga tcgtcgaggc cgtccatctc gccaccgaag
    14761 ggggagccga cggggtccag aagcaggggc tgcccccact ccccgagatc atggccgagt
    14821 acgtgtcgaa cgggggcgcg atctgggcct gccggtcctg cacgaagccg cgcggaatcg
    14881 aggaggggga cctctcgagg gagccaggat caagacgtcg atggaggtcg tggagctcct
    14941 cgcgggaggc gcggccagct tcgccttctg acctccgccg gccccctgct atgctcgggc
    15001 cgtggccctg gtgaccgtct tcggggcagg cgcggccggg accgcgctcg cgatccatct
    15061 ggcccgtaag ggcgaggacg cggtgctctg gggcagcgag cacgacgccg cggtgctcgg
    15121 cgatctcacg gagaagcgcc gccaccctgc ccttcccgag cgtctgcccg acgagctgcg
    15181 catcctcggt cccgacgacc ttgaggaggc cggcgcccac gcggagatcg ccgtgctcgg
    15241 ggccaactcg gccggcgccg ggccgctggg ccgcctcgtg gcccctgcct tgggcggcgc
    15301 tcggctcgcg gtctcgatcg ccaagggact gcagcccgag acgctggagc gcgtcagcgc
    15361 ggtctacgcg cgcgagctgc cctcgatcgc ggtcgtggcc atgagcggcc cctcactggc
    15421 ggcggagatc gccgaggggc tgctgaccgc cgtggcgttt gcctgcgagg acgagggggc
    15481 atgcaaggag gcggcggagg cgttccgctc ggaccgcttc ctcgtgagca cgaccgacga
    15541 cgtcgtgggc gtggag
//
LOCUS       DASUTZ010000193         4514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1747336, whole genome shotgun sequence.
ACCESSION   DASUTZ010000193 DASUTZ010000000
VERSION     DASUTZ010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1747336"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /gene="pafA"
                     /locus_tag="VF097_08495"
     CDS             complement(<1..1199)
                     /gene="pafA"
                     /locus_tag="VF097_08495"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HEX6262869.1"
                     /translation="MERRIFGLENEYGVTCTFRGQRRLSPDEVARYLFRRVVHWGRSS
                     NVFLENGARLYLDVGSHPEYATPECDSVDDLVAHDKAGERILERLLEAAELRLHEEGI
                     SGEIFLFKNNTDSAGNSYGCHENYLVARQGEFARMAEVLIPFFVTRQVFAGAGKVLHG
                     PRGATYCLSQRAEHIWEGVSSATTRSRPIINTRDEPHADAERYRRLHVIVGDSNMSEF
                     TTHLKIGVTDLVLRMVEDNTVMRDLTMENPIRAIREISHDITCRKRVRLTNGRELSAL
                     EIQAEYLERAMRYADHRGLDARGMAVLKDWEDVVTALRDGDLDRLRDRIDWVAKWHLI
                     EAYREKHDLPLSSPRVALMDLSYHDVNQDRGLYYLLERKAKMRRAVSEKAVQQAMSDP
                     PQTTRARL"
     gene            complement(1234..1611)
                     /locus_tag="VF097_08500"
     CDS             complement(1234..1611)
                     /locus_tag="VF097_08500"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX6262870.1"
                     /translation="MRTSTRLGALIAASVLLLAACGGDDDPTVEGGGDAGGAGDIQVA
                     MVDNSFAPSTLELTAGEEVTISTPNNGQNPHSFTIDDLGVDTGVLEAGEGSEVTFTVP
                     EGETQYYCTVHGAEVMSGTITAG"
     gene            complement(1662..2876)
                     /locus_tag="VF097_08505"
     CDS             complement(1662..2876)
                     /locus_tag="VF097_08505"
                     /inference="COORDINATES: protein motif:HMM:NF002367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6262871.1"
                     /translation="MSVEVRGIRPEEFDDLLRAVATGFGGHINDRDLALERRVMEIER
                     TVAATDGEVFVGGATASSMTLSVPGGIVPAAGITGVSVLPTHRRRGILTRMMRRSLDH
                     VREREPLSILWAAEGGIYGRFGYGMATRLMSLKVDRQHSAFRSDYSPQGSMRLVSRGE
                     AMKLIPPIYDRVAAEQPGFIGQTEEWTDHRFTIYDFLDDGFGKEFFFAIHEGSDGADG
                     YVVYRIKADWAGDERRTLKVEELVAATPGAYADVWRYVFDIDLVETSTAWDRPSHEPL
                     LQVFADPNAAKVELHDGLWVRLIRVDEALAARRYATGGRIVLEVDDSFCPWNRGRYEL
                     VGGPEGAECRPTDADPELRLTAELLGAAYLGGVSFRGLARAGLVQAAPDVLARADGMF
                     GWDPPPWCPIVF"
     gene            complement(2881..3567)
                     /gene="prcA"
                     /locus_tag="VF097_08510"
     CDS             complement(2881..3567)
                     /gene="prcA"
                     /locus_tag="VF097_08510"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011981501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HEX6262872.1"
                     /translation="MTLPFYVAPEQLMKDRSDYARKGIARGKPVVSLEYADGILFLAD
                     NPSTHLHKVSEIYDRIAFAGVGKYSEFENLRIAGVRLADIRGYSYGREDVTAKSLANA
                     YSQALSNVFTQEMKPYEVEILVAQLGDSNDENEMFHILYDGSVTDREGFVAMGGEADS
                     LTERLEASYRDGMDLGEALRAGVAALSEANEGGLEPSRIEAAVLDRTRKQRRKFRRLT
                     GEQVAGHLQG"
     gene            complement(3567..4358)
                     /gene="prcB"
                     /locus_tag="VF097_08515"
     CDS             complement(3567..4358)
                     /gene="prcB"
                     /locus_tag="VF097_08515"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HEX6262873.1"
                     /translation="MTEGVPGSFGANPSFYDLVRARGMGAPGTPGQTEGPGLAPPHGT
                     TVLALRFADGIVMAGDRRAVEGTSVADRRMDKVFPADDYSTVAIAGVAAQAIELVRLF
                     QTELEHYEKVEGEGLSLDGKANRLAQMIRSWMPMVMQGLIVVPVFAGYDLAKREGRIY
                     RYDVIGGRYEGIDFHATGSGGRDAKGSLKKRFRDGLDRGEAIRVALEALLDASEEDTA
                     TGGADVRRGIYPTVYVVTAEGADRVPEDEVRAAHEDVLERRTETG"
     gene            complement(4355..>4514)
                     /locus_tag="VF097_08520"
     CDS             complement(4355..>4514)
                     /locus_tag="VF097_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HEX6262874.1"
                     /translation="AEREAQAEGQTETAAKGEELKEEMDEILDEIDEVLEENAEEFIK
                     SYVQKGGE"
BASE COUNT          640 a         1625 c         1501 g          748 t
ORIGIN      
        1 agcctggccc gtgtggtctg cggcgggtcg ctcatcgcct gctggaccgc cttctcgctc
       61 accgcgcggc gcatcttcgc cttccgctcg aggaggtagt agaggccgcg gtcctggttc
      121 acgtcgtgat aggagaggtc catcagcgcc acccgggggc tggacagcgg caggtcgtgc
      181 ttctcccggt acgcctcgat cagatgccac ttcgcgaccc agtcgatgcg gtcccggagc
      241 cggtcgaggt cgccgtcccg gagcgccgtc acgacgtcct cccaatcctt gagcacggcc
      301 atgccccgcg cgtcgagccc gcggtggtcc gcgtaccgca tggcgcgctc caggtactcg
      361 gcctggatct cgagcgcgga gagctcgcgg ccgttggtga gccgcacgcg cttccggcag
      421 gtgatgtcgt ggctgatctc gcggatggcc cggatcgggt tctccatcgt gaggtcccgc
      481 atcacggtgt tgtcctcgac catacgcaga acgaggtcgg tcacaccgat cttgaggtgg
      541 gtggtgaact cgctcatgtt cgagtccccc acgatcacgt ggagccggcg gtaccgctcg
      601 gcgtcggcgt ggggctcgtc gcgcgtgttg atgatgggcc gggacctcgt cgtcgccgat
      661 gagaccccct cccagatgtg ctcggctcgc tgggagaggc agtaggtggc gccgcgcggc
      721 ccgtggagca ccttccccgc cccggcgaag acctgcctcg tgacgaagaa cgggatcagc
      781 acctcggcca tccgcgcgaa ctctccctgc ctggccacca ggtagttctc gtggcacccg
      841 taggagttcc cggccgagtc ggtgttgttc ttgaacagga agatctcgcc cgagatcccc
      901 tcctcgtgga ggcgaagctc ggcggcctcg agaaggcgct ccaggatccg ctctcccgcc
      961 ttgtcgtgcg ccacgaggtc gtccaccgag tcgcactcgg gcgttgcgta ctcgggatgc
     1021 gagcccacgt cgaggtagag ccgggcgccg ttctccagga acacgttcga cgagcgtccc
     1081 cagtggacca cgcgccggaa gaggtaccgc gcgacctcgt cggggctcag ccgtcgctgg
     1141 ccgcggaagg tgcaggtgac gccgtactcg ttctcgagcc cgaagatgcg ccgctccacc
     1201 cgtccagtgt aggcgcaggc cccgcggatg gcgtcagccg gccgtgatgg ttcccgacat
     1261 cacctccgcg ccatggaccg tgcagtagta ctgggtctcg ccctctggca cggtgaacgt
     1321 gacctcgctg ccctccccgg cctccaggac gccggtgtcc acgcccaagt cgtcgatcgt
     1381 gaatgagtgc gggttctggc cgttgttggg cgtcgagatc gtgacctcct cgcccgcggt
     1441 gagctcgaga gtgctgggcg cgaagctgtt gtcgaccatc gccacctgga tgtcgcccgc
     1501 gccgccggcg tcgccacctc cctcgacggt cggatcgtcg tcgccgccac acgctgccag
     1561 gaggaggacc gaggcggcga tcagggcgcc caaacgcgtg ctggttcgca tcgcttgcct
     1621 ttctcctcgg ggacgcccga ttctaacaac ggacctgatt tttagaagac gatcgggcac
     1681 cagggaggag gatcccaccc gaacatcccg tccgcccggg ccagaacgtc gggggcagcc
     1741 tggacgagcc cggcgcgcgc cagcccacgg aacgagacgc caccgaggta cgccgcgccc
     1801 agcagctcgg cggtgaggcg aagctctggg tcggcgtcgg tggggcgaca ctccgcgccc
     1861 tccgggccgc cgacgagctc gtagcggccc cggttccagg gacagaacga gtcgtccacc
     1921 tcgagcacga tgcggcctcc tgtcgcgtat cgacgcgccg ccagcgcctc gtccacacgg
     1981 atgagccgga cccagaggcc gtcgtgcagc tcgaccttgg ccgcgttggg gtccgcgaag
     2041 acctgcagca gcggctcgtg ggatggtcga tcccaggcgg tggaggtctc gacgaggtcg
     2101 atatcgaaaa cgtagcgcca cacgtcggcg taggctccgg gcgtggcggc gacgagctcc
     2161 tccaccttca gcgtgcgacg ctcgtctccc gcccagtcgg ccttgatgcg atagaccacg
     2221 tatccgtcgg ccccatccga cccctcgtgg atcgcgaaga agaactcctt cccgaaaccg
     2281 tcgtcgagga agtcgtagat cgtgaagcgg tggtcggtcc attcctcggt ctgcccgatg
     2341 aagcccggct gctcggcggc gacgcgatcg tagatcgggg ggatgagctt catcgcctcc
     2401 cccctcgaca ccaggcgcat cgatccctgg gggctgtaat ccgaacggaa cgcgctgtgc
     2461 tgtcggtcga ccttgaggct catcagcctc gtggccatcc catagccgaa ccggccgtag
     2521 atgccgccct ccgcggccca caggatcgag agcggctccc gctcacggac gtggtccagg
     2581 ctgcgccgca tcatccgggt caggatcccc cggcggcggt gggtggggag cacggacacg
     2641 cccgtgatgc cggcggcggg cacgatcccc cccgggaccg acagggtcat cgaggacgcg
     2701 gtggcgcccc cgacgaacac ctctccgtcg gtggcggcca cggtccgctc gatctccatc
     2761 acccggcgct cgagcgccag gtcccggtcg ttgatgtgcc cgccgaaccc cgtcgccacg
     2821 gcgcgcagca agtcgtcgaa ctcctcgggg cggatccccc gaacctcgac gctcatcccc
     2881 tcaaccctgg aggtggccgg ccacctgctc gccggtcagg cggcggaact tccggcgctg
     2941 cttccgggtt cggtcgagga cggcggcctc gatccgcgag ggctcgagcc cgccctcatt
     3001 cgcctccgac agtgccgcca cgcccgcgcg gagcgcctcg ccgaggtcca tgccgtcgcg
     3061 gtaggacgcc tcgagcctct cggtcaggga gtcggcctcg ccgcccatcg ccacgaagcc
     3121 ctcccggtcg gtgacgctgc cgtcgtagag gatgtggaac atctcgttct cgtcgttcga
     3181 gtcgccgagc tgggccacga ggatctcgac ctcgtagggc ttcatctcct gggtgaagac
     3241 gttcgagagc gcctgggagt aggcgttggc cagcgacttc gcggtgacgt cctcccggcc
     3301 gtaggagtac ccgcggatgt cggcgaggcg cacgcccgcg atcctgaggt tctcgaactc
     3361 cgagtacttc cccacaccgg cgaaggcgat ccggtcgtag atctcgctga ccttgtgcag
     3421 gtgggtggag gggttgtccg ccaagaacag gatgccgtcc gcatactcca gcgagaccac
     3481 cggcttgccg cgcgcgatcc ccttgcgcgc gtagtcggag cgatccttca tcagctgctc
     3541 gggggcgacg tagaagggca gggtcatcag cccgtctccg tcctgcgctc taggacgtcc
     3601 tcgtgggccg cccgcacctc gtcctcgggg acgcggtcgg ccccctcggc ggtcaccacg
     3661 tacacggtgg gatagatccc gcgccgcacg tcggctccgc cggtggcggt gtcctcctcg
     3721 gaggcgtcca ggagcgcctc gagcgccacc cggatcgcct ccccccggtc gagcccgtct
     3781 cggaaccgct tcttcaggct gcccttggcg tcgcggccgc ccgatcccgt cgcgtggaag
     3841 tcgatcccct cgtagcggcc gccgatcacg tcgtagcggt agatccgacc ctcccgcttg
     3901 gccaggtcgt agccggcgaa gaccggcacc acgatgagcc cctgcatcac catcggcatc
     3961 cacgagcgga tcatctgggc cagacggttg gccttcccgt ccagcgagag gccctccccc
     4021 tcgaccttct cgtagtgctc cagctcggtc tggaagagac ggaccagctc gatcgcctgc
     4081 gcggcgaccc cggcgatggc gaccgtggag tagtcgtcgg cggggaacac cttgtccatc
     4141 cggcggtcgg ccaccgacgt gccctccacc gcgcggcggt cgccggccat cacgatcccg
     4201 tcggcaaagc gtagggcgag gacggtcgtc ccgtgaggcg gcgcgagccc cggcccctcg
     4261 gtctggccgg gggtcccggg cgcgcccatc ccccgggccc ggacgagatc gtagaacgac
     4321 gggttggcgc cgaacgagcc gggaaccccc tccgtcattc gccgcccttc tggacgtagg
     4381 acttgatgaa ctcctcggcg ttctcctcga ggacctcgtc gatctcgtcg aggatctcgt
     4441 ccatctcctc cttgagctcc tcgcccttcg ccgcggtctc ggtctgcccc tcggcctggg
     4501 cctcgcgctc ggct
//
LOCUS       DASUTZ010000194         2729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_582832, whole genome shotgun sequence.
ACCESSION   DASUTZ010000194 DASUTZ010000000
VERSION     DASUTZ010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582832"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VF097_08525"
     CDS             complement(<1..630)
                     /locus_tag="VF097_08525"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6262875.1"
                     /translation="MGTRRYLLGKVAQATLTVLFVLVFNFFLFRVIPGDPAQILARAK
                     HLPKEAIVQLTEDFGLHEPIPKQFALYVEDTVTGNLGISYKFRRPVAGVIADRIWPTV
                     LLVGVSTLAATAIGLLIGVYGAWRRGSKLDLGSLGTSLVFYAMPEFWLGILLLMGFAA
                     GLGWFPTGGIRTPGAELTGVAAATDVLNHLFLPAITLTLALIGEYALIMR"
     gene            complement(658..2487)
                     /locus_tag="VF097_08530"
     CDS             complement(658..2487)
                     /locus_tag="VF097_08530"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6262876.1"
                     /translation="MTRRRGRGLAALTATALLIGLYAPGAGAQTPAPTAAEDEKITFV
                     YGDDNDIDSMNPWVGVEAPAYLMYALNYDLLVNFAIDDLSPSPGLAESWEVSEDGLTW
                     TFDIREGVTWHDGEPFTAHDVAFTFNRILEDRQGCCIDYIRQAETVEATDDTTLVITT
                     RRPTLQFLTAYVYILPEHIWSDISKEEAKTFENYPNPVGTGAFRVVEWEEGQFFRMEA
                     NPDYWAGAPKIDEVIYRIYQNEDALAQALLAGEVDFADTLGAGIYDSLEGQSDIGTNE
                     ANIPSFEEIGFNAGADDTYGDEADGHPALKDVTVRRAIAHAIDKETIVDRVVRGHGTV
                     GTTIVPEVTPFYHYEPTEEELIPFDLEEANRLLDEAGYEDTDDDGVREMPGGGQPLHF
                     RYFVRNEKQTTVTTSEFVTDWLEQIGISTEVRGMSDTRLTNVIYEGDFDMFHWGWFPD
                     ADPDFILSVLTCGQRPPDGIWSDSFYCNEEYDRLYEEQQTTLDLDERAEIVKEMQRIA
                     YEDAPYVVLFYDRSLQAYREDRWTGFRQQPADGGDLLAAYGPFSFISIRPKTAEEGGT
                     GQTGATSDTGIPTGVWLGLIGAVLVVAIIWLLLRRRGAEEERE"
     gene            complement(2484..>2729)
                     /locus_tag="VF097_08535"
     CDS             complement(2484..>2729)
                     /locus_tag="VF097_08535"
                     /inference="COORDINATES: protein motif:HMM:NF036772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262877.1"
                     /translation="GEMYRSEEWRHPEPWLRSRSMEDVLEYHLGPLGVPVLTNLPLGH
                     GKHLATIPLGVEVTVDADERRLIVEESALQEAEGGER"
BASE COUNT          397 a          940 c          921 g          471 t
ORIGIN      
        1 ccgcatgatc agcgcgtact cgccgatcag cgccagcgtc agcgtgatag ccggcaggaa
       61 cagatggttg aggacgtcgg tggctgcggc gacccccgtg agctcggcgc cgggcgtccg
      121 gatccccccg gtcgggaacc agcccaggcc ggccgcgaac cccatcagga gcaggatccc
      181 cagccagaac tcgggcatcg catagaagac gagggacgtc cccagcgatc cgaggtcgag
      241 cttgctccca cgtcgccacg ctccgtagac gccgatcagg agaccgatcg cggtggccgc
      301 cagcgtggag acgcccacga gcagaaccgt gggccagatg cgatcggcga tcacccccgc
      361 caccggcctc cggaacttat acgagatccc caggttcccc gtgacggtgt cctcgacgta
      421 cagcgcgaac tgtttcggga tcggctcgtg cagcccgaag tcctcggtga gctggacgat
      481 cgcctccttg ggcaggtgct tcgcgcgcgc gaggatctgc gcggggtcgc ccgggatgac
      541 gcggaacagg aagaagttga agacgaggac gaacagcacg gtcagggtcg cctgggccac
      601 cttcccgagc aggtagcgtc tcgtccccat ggggcctccc gtcccgcgcc cggctcccta
      661 ctcgcgctcc tcttcggcgc ctcgacggcg cagcagcaac cagatgatgg ccacgaccag
      721 cacggccccg atcaagccga gccagacgcc ggtggggatg ccggtgtccg aggtggcccc
      781 agtctgtccg gtgcccccct cctccgcggt cttgggccgg atgctgatga agctgaacgg
      841 cccgtacgcg gccaggaggt cgccgccgtc cgccggctgc tgccggaacc cggtccagcg
      901 gtcctcgcgg taggcctgga ggctccggtc gtagaacagc acgacgtacg gagcgtcctc
      961 gtaggcgatc cgctgcatct ccttgacgat ttcggcccgc tcgtcgaggt cgagcgtcgt
     1021 ctgctgctcc tcgtacaggc ggtcgtactc ctcgttgcag tagaaggagt cgctccagat
     1081 gccgtccggc gggcgctggc cgcatgtcag caccgacagg atgaagtcgg ggtcggcgtc
     1141 ggggaaccag ccccagtgga acatgtcgaa gtcgccttcg tagatcacgt tcgtgagccg
     1201 cgtgtcgctc atcccgcgga cctcggtgga gatgccgatc tgctcgagcc agtcggtgac
     1261 gaactcgctc gtcgtcaccg tcgtctgctt ctcgttccgc acgaagtagc ggaagtggag
     1321 tggctgcccc ccgcccggca tctcgcgcac gccgtcgtcg tcggtgtcct cgtaccccgc
     1381 ctcgtccagc aggcggttgg cctcctcgag atcgaagggg atcagctcct cctcggtcgg
     1441 ctcgtagtgg tagaagggtg tcacctccgg gacgatcgtg gtgccgaccg tgccgtggcc
     1501 ccggaccacg cggtcgacga tcgtctcctt gtcgatggcg tgggcgatgg cccggcgtac
     1561 ggtgacgtcc ttgagggcgg ggtgaccgtc cgcctcgtcg ccgtaggtgt cgtcggcccc
     1621 cgcgttgaac ccgatctcct cgaagctggg gatgttggcc tcgttcgtcc cgatgtcgga
     1681 ctggccctcg agcgagtcgt agatgccggc cccgagggtg tcggcgaagt ccacctcgcc
     1741 ggccaggagg gcttgggcca gggcgtcctc gttctggtag atgcggtaga tcacctcgtc
     1801 gatcttcggg gcgcccgccc agtagtcggg gttggcctcc atccggaaga actggccctc
     1861 ctcccactcg acgacgcgga aggcgcccgt ccccacgggg ttcgggtagt tctcgaaggt
     1921 cttggcctcc tccttggaga tgtcgctcca gatgtgctcg gggaggatgt agacgtacgc
     1981 ggtgaggaac tgcagggtcg gtcgccgcgt ggtgatcacg agggtagtgt cgtcggtggc
     2041 ctcgaccgtc tccgcctgcc ggatgtagtc gatgcagcag ccctgccggt cctcgaggat
     2101 ccggttgaag gtgaaggcca cgtcgtgcgc cgtgaagggc tcgccgtcgt gccacgtcac
     2161 gccctcgcgg atgtcgaagg tccaggtcag cccgtcctcg ctcacctccc acgactcggc
     2221 caggccgggt gagggcgaga ggtcatcgat cgcgaagttc accaggaggt cgtagttgag
     2281 cgcgtacatc aggtaggcgg gcgcctccac gccgacccac gggttcatcg agtcgatgtc
     2341 gttgtcgtcg ccgtagacga aggtgatctt ctcgtcctcg gcggccgtcg gcgccggcgt
     2401 ctgtgccccg gcccccggcg cgtacagacc gatcagcaga gccgtcgcgg tcagtgcggc
     2461 cagccctcgt ccccttctgc gcgtcatcgc tcccctcctt ccgcctcctg gagggccgac
     2521 tcctccacga tcaggcgtcg ctcgtccgcg tccacggtca cctcgacccc cagcgggatc
     2581 gtggccaggt gcttcccgtg ccccaggggc aggttggtca gcaccgggac cccgaggggc
     2641 ccgaggtggt actccagcac gtcctccatc gagcggctcc ggagccaggg ctccggatgc
     2701 cgccactcct cgctgcggta catctcgcc
//
LOCUS       DASUTZ010000195         7788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_971923, whole genome shotgun sequence.
ACCESSION   DASUTZ010000195 DASUTZ010000000
VERSION     DASUTZ010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7788
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971923"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /gene="miaA"
                     /locus_tag="VF097_08540"
     CDS             <1..871
                     /gene="miaA"
                     /locus_tag="VF097_08540"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018785978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX6262878.1"
                     /translation="ELARRLDAEVILVDSMTVYRGMDVGTAKPGAEERAAVPHHLVDV
                     ADPTEPFNVALFQRMARAAVVAIAARGRTPLLVGGSGLYFRAVVDDLEFPGTDSGLRA
                     LLETEGRALGPEWMHARLAGLDPDAAARIEPQNLRRSVRALEVAALTGRPFSSFSAAW
                     ELYPQDRVRAAGLRLSADLLRARIEARARRLLEEGLVEEVRALLDRGFGPFLTASQAI
                     GYLEIVRHLEDRLTLEEAAREIARRTRALARRQMAWFRRDPRIRWFDVDEGGPPLDRI
                     EKHLRGAAPGRGR"
     gene            868..1758
                     /gene="dapF"
                     /locus_tag="VF097_08545"
     CDS             868..1758
                     /gene="dapF"
                     /locus_tag="VF097_08545"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX6262879.1"
                     /translation="MKVSKYHGTGNDFVMWVDLDDRDRQTPELVAALCDRHLGVGGDG
                     TIRITGSDRADFFMDYWNASGETAEMCGNGIRCLAKLVYERGLTTATEIDVDTRAGVK
                     RLVLTVEDGVVEAVRVDMGPPAFERSVLPMRGEGTFLDQAFEVEGATYRGFGISMGNP
                     HLVLIGDRDPEELDVRRIGPDLEHHEDFPQRTNVEFVRVVDGVVEVRVWERGSGETMA
                     CGTGACASLVAANLAGLAPREAPVRFPGGELRLAWADDDRVFLTGPATFVFDAELSPA
                     WVERARAGRVASPTPEGVAT"
     gene            1755..2909
                     /locus_tag="VF097_08550"
     CDS             1755..2909
                     /locus_tag="VF097_08550"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HEX6262880.1"
                     /translation="MRIASRLDRLPPYMFAELDRKIEGKRAAGVDVISLGVGDPDTPT
                     PEHVVDALREAATDPATHRYPSYFGMPELRRAIAGHYRDRFGVELDPDAEVQPLIGSK
                     EGLAHLPIAFVDPGDEALVPDPGYPVYASSTTLAGGTPVAFPLSADRGFLPDLDAMPV
                     GDRSRILWLNYPSNPTAAVADLAFLERAVAFAREHDLLLVHDAAYSEMTYEGYVAPSV
                     LQVPDAKDVAIEFGSVSKTHNMTGWRVGWAVGSPEAVRALAQVKTNIDSGIFDAVQRA
                     AIAAITGPQDHLERLRALYQKRRDVVIATLNDLGWDLEPPKGSFYIWFPTKDGMGSAE
                     FCDLVLERTGVVISPGSGYGAGGEGYARISLTVPDDRLQEAMDRFREALS"
     gene            2996..4225
                     /gene="hflX"
                     /locus_tag="VF097_08555"
     CDS             2996..4225
                     /gene="hflX"
                     /locus_tag="VF097_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018504638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX6262881.1"
                     /translation="MGIAPDEGDLDELAALADTAGAEVVGRLVQPRDAPDPRTFIGKG
                     RLGDLHALVHDTDAGLVIFDQELTPGQLRHLEEDLGVKVVDRTALILDIFALHARSRE
                     GKLQVELAQLSYLLPRLRGWGEAMSRMGGGIGTRRGPGETKMEVDRRRIRQRISRLRR
                     ELRDVARSRDVTRSRRERSRIPQIAIAGYTNAGKSTLMNALTGADALVADQLFATLDP
                     TVRRLRLPGGRGVTLSDTVGFVGKLPHDLVEAFRSTLEEVTRADLILHVADASAADVD
                     EQVAAVREVLAEIDAAQLPEVLALNKADLVSAEDRARLEPRFPDAVLVSALEGDGLDK
                     LLARVDDVLPSPPVEVHLRIPYARQDVTARLHREAEVLSVEPGEEDTLVDVRLREDQV
                     TWVEEFLVQSPRVRRSG"
     gene            4233..5036
                     /locus_tag="VF097_08560"
     CDS             4233..5036
                     /locus_tag="VF097_08560"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6262882.1"
                     /translation="MDLPDQEWVRRVVERYTADPSAYRDCWAPVLVTLSARLARRLPL
                     GAARRVLDLGAGTGALLPELRGLAPRADVVAADVTEAMLREADPSTPRVVADAQHLPF
                     AADTFDVVVMAFMLFHLPDPVAGLREARRVLGPGGSVGVATWTDHDLCPPLAAWEEEL
                     DAHGAAPTPSLSAHERTDAPEKVRGLVEAAGLEMLSVETARANVRLSRDEFWGQRVRL
                     STSRERLASLDEVARAACLAAVGKRLDALPDTAFVDRSEAVLAVARVPI"
     gene            complement(5037..5648)
                     /gene="lexA"
                     /locus_tag="VF097_08565"
     CDS             complement(5037..5648)
                     /gene="lexA"
                     /locus_tag="VF097_08565"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX6262883.1"
                     /translation="MDDITPRQQRILDFIRATVRDRGYPPTVREIGEAVGLTSSSSVH
                     AQLSNLQRKGLLRRDPSKPRTIELAEHRATSATSVPLLGRVAAGAPILADENLEDHIA
                     VPTDFAGGGELFALRVEGDSMVEAGILDGDVVVVRRQDVAADGDIVAALVPGPAEHEA
                     TVKRLRRGGGGLTLVPENPAMEPFPMPEGGRVLGVVTAVLRKV"
     gene            5754..6047
                     /locus_tag="VF097_08570"
     CDS             5754..6047
                     /locus_tag="VF097_08570"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEX6262884.1"
                     /translation="MGRTRVRTGRIAALIAAISVTVVLISGAASGRIEAPAGGSGGDV
                     HVVAQGDTLWGIARDLVGPEGDPRPLVQDLRDLNGLGPSDVLMPGLPLALPAP"
     gene            complement(6044..6724)
                     /locus_tag="VF097_08575"
     CDS             complement(6044..6724)
                     /locus_tag="VF097_08575"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX6262885.1"
                     /translation="MPQDWELALRLLAAAGLGGLIGFEREIRDQPAGFRTHILVAVGS
                     CLFAIVSAYGFDAFLGFRPQEIRFDPSRVAAQIVTGIGFLGAGAIIRYGMTVRGLTTA
                     ASLWIVAAIGLAVAVGQWITAGTATAITLVALWALRHIRPRLTRGLKPEHEEFFLDLE
                     PTTSLESVVRHVHEAGLRIDHLQIEEGGEGERAVSLVLRLPPDRSPEDVAALLTEAPG
                     IRNVGWTR"
     gene            6797..7264
                     /gene="nrdR"
                     /locus_tag="VF097_08580"
     CDS             6797..7264
                     /gene="nrdR"
                     /locus_tag="VF097_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX6262886.1"
                     /translation="MRCRYCGAEGTRVVDSRAAEEGRTIRRRRACPSCGRRFTTYERA
                     DGAGLTVVKLDGRKEPYDRSKVVSGVMKAIKNRPVDETQVSRLVDEVERRLERKGPVV
                     TTQEVGVEVLDQLRKLDEIAYIRFASVYKDFQELTDFERELGLLLQKRGPKEG"
     gene            7430..>7788
                     /locus_tag="VF097_08585"
     CDS             7430..>7788
                     /locus_tag="VF097_08585"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674281.1"
                     /note="catalyzes the reduction of ribonucleotides to
                     deoxyribonucleotides; the rate-limiting step in dNTP
                     synthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEX6262887.1"
                     /translation="MATKDLDRKTRGLEFERYFTGEGVHPFDEIEWETRDAVIPNLKT
                     GGNAFEQRGVEFPTSWSQNATNIVAQKYFRGPLGTPQRESSVRQLIGRVVDTIRGWGE
                     KDGYFATERDAVVFSEEL"
BASE COUNT         1089 a         2770 c         2833 g         1096 t
ORIGIN      
        1 cgagctggcg cgaaggctcg acgccgaggt gatcctcgtg gactcgatga ccgtctaccg
       61 ggggatggac gtcggcaccg cgaagcccgg tgccgaggag cgagccgccg tcccgcacca
      121 tctcgtcgac gtcgccgacc ctacggagcc cttcaacgtg gcgctgttcc agcggatggc
      181 ccgggccgcg gtcgtcgcga tcgccgctcg cgggcggacc ccactgctcg tgggaggatc
      241 gggcctctac ttccgcgcgg tcgtggacga cctcgagttc cccggcaccg actcggggct
      301 ccgcgcgctc ctcgagaccg agggccgcgc cctggggccc gagtggatgc acgcccgcct
      361 ggccgggctc gatcccgacg cggcggcgcg gatcgagccc cagaacctcc ggcgcagcgt
      421 ccgcgcgctc gaggtcgccg cgctcaccgg caggccgttc tcatcgttct ccgcggcctg
      481 ggagctctac cctcaggacc gcgtccgcgc cgcgggcctt cgactctccg ccgatctcct
      541 ccgcgcgcgg atcgaggccc gcgcgcgccg cttgctcgag gaggggctcg tggaggaggt
      601 ccgtgcgctc ctggaccgcg gcttcggccc cttcctcacg gcatcccagg ccatcgggta
      661 ccttgagatc gtgcggcacc tcgaggatcg cctcacgctc gaggaggcgg cccgcgagat
      721 cgcacggcgc acgcgggcgc tggctcgtcg ccagatggcc tggttccgtc gcgacccgcg
      781 gatccgctgg ttcgacgtgg acgagggggg gccgccgctg gatcgcatcg agaagcacct
      841 gcggggcgcg gccccgggga ggggacgatg aaggtatcga agtaccacgg gaccgggaac
      901 gacttcgtga tgtgggtcga tctcgacgac cgtgaccgcc aaacgcccga gctcgtggcg
      961 gccctctgcg accgccacct gggcgtggga ggcgacggga cgatccgcat cacggggtcg
     1021 gaccgggccg acttcttcat ggactactgg aacgcgagcg gcgagacggc cgagatgtgc
     1081 gggaacggga tccgctgcct ggccaagctc gtctacgagc gggggctgac caccgccacc
     1141 gagatcgacg tggacacccg tgcgggcgtg aagcgcttgg tcctcaccgt ggaggacggc
     1201 gtggtcgaag ccgtccgcgt ggacatgggc cccccggcgt tcgagcggtc cgtgctcccc
     1261 atgcggggcg aagggacctt cctcgaccag gccttcgagg tcgagggggc gacgtatcgg
     1321 ggcttcggga tctcgatggg gaacccccac ctcgtcctca tcggcgatcg ggaccccgag
     1381 gagctcgacg tgcggaggat cgggccggac ctggagcacc acgaggactt cccgcagcgg
     1441 acgaacgtgg agttcgtccg ggtcgtggac ggcgtggtcg aggtccgggt ctgggagcgc
     1501 gggtcggggg agacgatggc ctgcggcacc ggggcctgcg cctcgctcgt cgcggccaac
     1561 ctcgcggggc tcgcgccccg ggaggccccc gtccggttcc ccggcggcga gctccggctc
     1621 gcgtgggccg atgacgaccg cgtcttcctc accggcccgg ccaccttcgt gttcgacgcg
     1681 gagctctccc ctgcctgggt ggagcgggcc cgggccgggc gcgtcgcgtc cccgacgccg
     1741 gagggggtgg ccacgtgagg atcgcctcgc gcctcgatcg cctgccgccg tacatgttcg
     1801 ccgagctgga ccggaagatc gaggggaagc gcgcggccgg cgtggacgtg atctcgctcg
     1861 gcgtcggcga cccggacacc cccacccccg agcacgtcgt ggacgccctg cgggaggccg
     1921 cgaccgatcc cgcgacgcac cggtacccct cgtacttcgg gatgcccgag ctgcgaaggg
     1981 cgatcgccgg ccactaccgg gaccggttcg gggtggagct cgatccggac gccgaggtgc
     2041 aaccgctgat cgggtcgaag gaggggctgg cccacctgcc gatcgcgttc gtcgaccccg
     2101 gcgacgaggc gctcgtcccc gaccccggct accccgtgta cgcgtcgagc accacgctgg
     2161 cgggggggac gccggtggcc ttccccctgt ccgccgaccg tggcttcctc ccggacctcg
     2221 acgcgatgcc ggtgggggac cggagccgga tcctgtggct caactacccc tcgaacccga
     2281 ccgcggccgt ggccgacctc gccttcctcg agcgggccgt ggccttcgcc cgcgagcacg
     2341 acctgctcct ggtccacgac gccgcttact cggagatgac ctacgagggc tacgtggccc
     2401 cctcggtgct ccaggtcccg gatgcgaagg acgtggccat cgagttcggc tcggtctcca
     2461 agacccacaa catgacgggc tggcgcgtgg ggtgggccgt cggcagcccg gaagccgtcc
     2521 gggccctggc ccaggtcaag acgaacatcg actcggggat cttcgacgcg gtccagcggg
     2581 cggcgatcgc ggcgatcacc ggcccccagg accacctgga gcgcctccgg gcgctgtacc
     2641 agaagcggcg ggacgtcgtg atcgccacgc ttaacgacct cgggtgggac ctcgagccgc
     2701 cgaagggatc cttctacatc tggttcccca cgaaggacgg gatggggtcg gccgagttct
     2761 gcgacctcgt gctggagcgg accggcgtcg tgatctcacc cggcagcggc tacggggccg
     2821 ggggagaggg ctacgcgcgg atctcgctga ccgtccccga cgaccggctg caggaggcga
     2881 tggaccggtt ccgagaggcg ctctcgtgag cgggcgccct ccgaggcgac gaagggtctc
     2941 cgagtccccc ctctcagccc ggtggcggcc gaccacctcc gagcccgcgg tcctcgtggg
     3001 gatcgctccc gacgagggcg atctcgacga gctcgctgcc ctggccgaca ccgcgggcgc
     3061 ggaggtggtc ggccgcctgg tccagccccg cgacgccccc gacccgagga ccttcatcgg
     3121 gaaggggagg ctcggcgacc tccatgccct cgttcacgac accgacgcgg ggctcgtgat
     3181 cttcgaccag gagctgacgc cggggcagct gcgccacctc gaggaggatc tcggtgtgaa
     3241 ggtggtcgac cgcaccgcgc tgatcctgga catcttcgcg ctccacgccc gctccaggga
     3301 gggcaagctc caggtcgagc tggcccagct cagctacctc ctcccgcgtc tgcggggttg
     3361 gggagaggcg atgtcgagga tgggcggggg catcgggacg cgtcgcggcc cgggcgagac
     3421 gaagatggag gtggaccgcc ggcgcatccg ccagcggatc tctcggctcc gtcgcgagct
     3481 gcgcgacgtg gcccgctccc gcgacgtgac caggtctcgc cgggagcgct cccggatccc
     3541 acagatcgcg atcgccgggt acacgaacgc ggggaagtcc acgctgatga acgcgctcac
     3601 cggcgcggac gcgctcgtcg ccgaccagct cttcgccacg ctggatccga cggtgcggag
     3661 gctccggctc cccggcggcc gcggcgtcac cctctcggac accgtggggt tcgtggggaa
     3721 gctcccccac gacctcgtgg aggccttccg ctccaccctc gaggaggtga cgagggccga
     3781 cctgatcctc cacgtggcgg acgcgtccgc cgccgacgtc gacgagcagg tcgccgccgt
     3841 gcgcgaggtg ctggccgaga tcgacgccgc ccagctgccc gaggttctcg ccctcaacaa
     3901 ggccgatctg gtctccgccg aggaccgggc ccggctcgag ccgcgcttcc ccgatgcggt
     3961 gctcgtgtcc gcgctcgagg gcgacgggct ggacaagctg ctggcccgcg tggacgacgt
     4021 cctcccgtcc ccgccggtcg aggtgcacct gcggatcccg tacgcccgcc aggacgtcac
     4081 ggcgcggctt caccgtgagg ccgaggtgct gagcgtggag ccgggggagg aagacaccct
     4141 cgtggacgtc cgcctccgcg aggaccaggt cacctgggtc gaggagttcc tcgtgcagtc
     4201 gccgcgggtg cgccgctccg gctgagggcc ggatggacct tcccgaccag gagtgggtcc
     4261 ggagggtcgt cgaacggtac acggcggacc ccagcgctta ccgcgactgc tgggcccccg
     4321 tcctggtcac gctcagcgcc cgcctcgccc gccgtctccc gctcggcgcc gcccgccgcg
     4381 tgctcgatct cggagccgga acgggcgccc tgctgcccga gctccgcggt ctcgctcccc
     4441 gggcggacgt cgtggccgcg gacgtcaccg aggccatgct gagggaggcc gacccctcga
     4501 ccccgagggt cgtggccgat gcgcagcatc tcccgttcgc cgccgacacg ttcgatgtgg
     4561 tcgtgatggc cttcatgctg ttccacctcc ccgaccccgt cgccgggctg cgcgaggctc
     4621 ggcgggtgct cgggcccggc gggtcggtgg gggtggcgac gtggacggac cacgatctgt
     4681 gcccgcccct ggcggcgtgg gaggaggagc tggacgcgca cggggcggct cccacgccga
     4741 gcctctcggc ccacgagcgg acggacgccc cagagaaggt ccgcggcctc gtggaggcgg
     4801 ccgggctcga gatgctgtcc gtcgagacgg cgcgagcgaa cgtccggctc tcgcgcgatg
     4861 agttctgggg ccagcgggtg cgcctctcca cctcccggga gcgtctggcg tccctcgatg
     4921 aggtggcgcg ggcggcgtgc ctggccgcgg tcgggaagcg cctcgacgcg ctcccagata
     4981 cagccttcgt cgatcggtcg gaggcggtcc tggcggtggc ccgcgtcccg atctagctag
     5041 accttccgca ggacggccgt gacgactccc agcacccgcc cgccctcggg catgggaaag
     5101 ggctccatgg ccgggttctc cgggacgagc gtgaggccgc ccccgccgcg ccggaggcgc
     5161 ttcaccgtgg cctcgtgctc cgcgggcccg gggacgagcg cggccacgat gtcgccgtcc
     5221 gcggcgacgt cctggcgccg cacgaccacc acgtcgccgt cgaggatccc ggcctcgacc
     5281 atcgaatcac cctccacgcg aagggcgaag agctctccgc ctccggcgaa gtcggtgggc
     5341 accgcgatgt ggtcctccag gttctcgtcg gccaggatcg gcgccccggc cgccacgcga
     5401 cccagcaggg ggaccgaggt cgcgctcgtg gcgcggtgtt cggcgagctc gatcgtcctc
     5461 ggcttggagg ggtcgcggcg gagcagcccc ttccgctgga gattcgagag ctgggcgtgc
     5521 acgctggagg acgaggtgag gccgaccgcc tccccgatct cccgcacggt cggcggatac
     5581 cctcgatccc gcaccgtggc ccggatgaag tcgaggatcc gctgctgccg tggcgtgatg
     5641 tcgtccatgc cgacctcctc ttggtccggt cgacgctagc acgcgaaggg gcctcaagca
     5701 aacatctgtt cgagtgcttg acgctcgaac gggtgttcgg catacttccc cgcatgggtc
     5761 gaacacgggt tcgaacgggg aggatcgccg cgcttatcgc ggcgatctcc gtgacggtgg
     5821 tcctgatctc gggggccgcc agtggccgga tcgaggcgcc cgcgggagga tcgggcggag
     5881 acgtccacgt cgtggcccag ggggacaccc tgtggggcat cgccagggac ctcgtgggac
     5941 ccgagggcga cccccgcccg ctcgtgcagg acctccgcga cctgaacggc ctgggcccga
     6001 gcgacgtcct gatgcccggc ctcccgctcg ccctccccgc tccctagcgg gtccacccca
     6061 cgttccggat ccccggggcc tccgtgagca gcgcggctac gtcctcgggg ctgcgatccg
     6121 gcgggagccg caggacgagg gagacggcgc gctcgccctc gcccccctcc tcgatctgga
     6181 ggtggtcgat gcggaggccc gcctcgtgga cgtgacggac gaccgactcc agcgaggtcg
     6241 tcggctccag gtcgaggaag aactcctcgt gctcgggctt gaggccccgg gtcagcctgg
     6301 ggcggatgtg gcggagcgcc cagagggcga ccagcgtgat ggcggtggcc gtgcccgcgg
     6361 tgatccactg cccgaccgcg acggcgaggc cgatcgcggc cacgatccac aggctggcgg
     6421 ccgtggtcag gccgcgaacg gtcatcccgt agcggatgat cgccccggcc ccgaggaagc
     6481 cgatcccggt cacgatctgg gcggccaccc tcgagggatc gaagcggatc tcctggggcc
     6541 ggaacccgag gaaggcgtcg aagccgtagg ccgacacgat ggcgaagagg cacgagccga
     6601 cggccacgag gatgtgcgtg cggaagccgg ccggctggtc gcggatctct cgctcgaacc
     6661 cgatcaggcc gcccagcccg gcggcggcga gcagtcggag cgcgagctcc cagtcctggg
     6721 gcatgaccgg attatcgcag gggagggccg cccccacatc ttgaggtccc cttctcgacg
     6781 gtccaagatg ttgtgcatgc gatgccggta ctgcggggcc gagggcaccc gggtcgtgga
     6841 ctcccgggcc gcggaggagg gccggacgat ccggcggcgt cgggcgtgtc cctcgtgcgg
     6901 ccggaggttc accacgtacg agcgcgccga cggggccggc ctgaccgtgg tcaaactgga
     6961 cggccgcaag gaaccctacg accggtccaa ggtggtctcg ggggtgatga aggcgatcaa
     7021 gaaccggccg gtggacgaga cgcaggtctc ccgtcttgtg gacgaggtcg agcgacggtt
     7081 ggagcgcaag ggcccggtcg tcaccacgca ggaggtgggc gtggaggtcc tcgaccagct
     7141 ccggaagctc gacgagatcg cctacatccg cttcgcctcg gtctacaagg acttccagga
     7201 gctcacggac ttcgagcgcg agctgggcct cctgctgcag aagaggggcc ccaaggaggg
     7261 gtgaccggcc ctccacacgc tttccacagg ttctccacag aaaccacaac atcttgtggt
     7321 cgcgatccgt agacgccaca acatcatccg tcgtagactc ttgcaaggac cggccgacga
     7381 gcgtagaatc acgccactgt aattcgcacc ccggaaggag gcgtcagccg tggcgaccaa
     7441 ggacctcgat cgcaagacaa gggggctcga gttcgagcgc tacttcaccg gcgagggggt
     7501 gcacccgttc gacgagatcg agtgggagac tcgcgacgcg gtgatcccca acctcaagac
     7561 cggcgggaac gccttcgagc agcgcggggt ggagttcccc acgtcctggt cccagaacgc
     7621 cacgaacatc gtggcccaga agtacttccg tggcccgctc ggcacccccc agcgcgagtc
     7681 ctcggtccgc cagctgatcg gcagggtcgt ggacacgatc cggggctggg gcgagaagga
     7741 cggctacttc gccaccgagc gcgacgccgt ggtcttctcc gaggagct
//
LOCUS       DASUTZ010000196        13167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_583400, whole genome shotgun sequence.
ACCESSION   DASUTZ010000196 DASUTZ010000000
VERSION     DASUTZ010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13167
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583400"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..2775)
                     /locus_tag="VF097_08590"
     CDS             complement(49..2775)
                     /locus_tag="VF097_08590"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEX6262888.1"
                     /translation="MATKDLDRKTKGLEFERYFTSEGVHPFDEIEWETRDAVIPNLKT
                     GGNAFEQRGVEFPKSWSQNATNIVAQKYFRGPLGTPQRESSVRQLIGRVVDTIRGWGE
                     KDGYFATERDATVFSEELSHLLVTQKAAFNSPVWFNVGAEEKPQCSACFILSVEDTMT
                     GILNWYVEEGTIFKGGSGSGINLSTIRSSKERLSSGGLASGPVSFMRGADASAGTIKS
                     GGKTRRAAKMVILDVDHADVEEFIWCKAVEERKARVLRENGFDMDLDGVDSHSTQYQN
                     ANNSVRVTDEFMKAHLEDRDWNLKAVLDGSTVKSVRARDVMRQIAQAAWECADPGMQY
                     DTTINDWHTCPATGRINASNPCSEYMHLDNSACNLASLNLLKFLNHDGSFDIQAFKRA
                     VEVVFTAQEILVGNSSYPTEKIERNAKAFRQLGLGYANLGGLLMARGLAYDSDQGRAW
                     AGAITAIMTGHAYATSARIAEKMGPFAGYAPNRDAMLRVMRKHRAGADEIDADQVPEG
                     LLSAAKQAWDEAIGLGEQFGYRNAQASVLAPTGTIGLMMDCDTTGIEPDLALVKTKKL
                     VGGGTMQIVNRTVPRALETLGYTEDQTKDIVGYIAEHNSVKGAPHLRPEHESVFDTSM
                     GEQAIHYMGHVRMMAAAQPFISGAISKTVNMPEEATVEDVEQLFLEGWRLGLKAVAIY
                     RDNCKVAQPLSADKKKSATEPTTAPAGTPVRRRLPSTRPGKTFSFSVGEADGYIIAGE
                     YPEDGIGEIFLKVAKQGSTLAGIMDAFAISVSLGLQYGVPLEAYVQKFTNMRFEPSGI
                     TSDADIRFATSLVDYIFRRLALEYLPPETRKALGVHSIEERKETASEHAGAAGADPAP
                     QVVDVSVELDEKQGTAEAKAKDAPLCYSCGSRMQPAGSCYVCNSCGATSGCS"
     gene            complement(2941..3408)
                     /gene="nrdR"
                     /locus_tag="VF097_08595"
     CDS             complement(2941..3408)
                     /gene="nrdR"
                     /locus_tag="VF097_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX6262889.1"
                     /translation="MRCRYCGADDTRVVDSRAAEDGRSIRRRRACPSCGRRFTTYERA
                     DGAGLTVVKLDGRKEPYDRSKVVSGVMKAIKNRPVDETQVLRLVDEVERRLERKGPVV
                     TTQEVGVEVLDQLRKLDEIAYIRFASVYKDFQELTDFERELGLLLQKRSPKEG"
     gene            3481..4161
                     /locus_tag="VF097_08600"
     CDS             3481..4161
                     /locus_tag="VF097_08600"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX6262890.1"
                     /translation="MPQDWELALRLLAAAGLGGLIGFEREIRDQPAGFRTHILVAVGS
                     CLFAIVSAYGFDAFLGFRPQEIRFDPSRVAAQIVTGIGFLGAGAIIRYGMTVRGLTTA
                     ASLWIVAAIGLAVAVGQWITAGTATAITLVALWALRHIRPRLTRGLKPEHEEFFLDLE
                     PTTSLESVVRHVHEAGLRIDHLQIEEGGEGERAVSLVLRLPPDRSPEDVAALLTEAPG
                     IRNVGWTR"
     gene            complement(4158..4451)
                     /locus_tag="VF097_08605"
     CDS             complement(4158..4451)
                     /locus_tag="VF097_08605"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEX6262891.1"
                     /translation="MGRTRVRTGRIAGLIAAVSVTVVLISGAASGRIEAPAGGSGGDV
                     HVVARGDTLWGIARGLVGPEGDPRPLVQDLRDLNGLGPGDVLVPGLRLALPAP"
     gene            4556..5167
                     /gene="lexA"
                     /locus_tag="VF097_08610"
     CDS             4556..5167
                     /gene="lexA"
                     /locus_tag="VF097_08610"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX6262892.1"
                     /translation="MDDITPRQQRILDFIRATVRDRGYPPTVREIGEAVGLTSSSSVH
                     AQLANLQRKGLLRRDPSKPRTIELAEHRAASATSVPLLGRVAAGAPILADENLEDHIA
                     VPTDFAGGGELFALRVEGDSMVEAGILDGDVVVVRRQDVAADGNIVAALVPGPAEHEA
                     TVKRLRRGGGGLTLVPENPALEPFPMPEGGRVLGVVTAVLRKV"
     gene            complement(5168..5971)
                     /locus_tag="VF097_08615"
     CDS             complement(5168..5971)
                     /locus_tag="VF097_08615"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6262893.1"
                     /translation="MDLPDQEWVRRVVERYTADPSAYRDCWAPVLVTLSARLARRLPL
                     GAARRVLDLGAGTGALLPELRGLAPRADVVAADVTEAMLREADPSIPRVVADAQRLPF
                     AAGTFDVVVMAFMLFHLPDPVAGLREARRVLGPGGSVGVATWTDHDLCPPLAAWEEEL
                     DAHGAAPTPSLSAHERTDAPEKVRGLVEAAGLEMLSVETARANLRLSRDEFWGQRVRL
                     STSRERLGSLDEVARAACLAAVGKRLDALPDRAFVDRSEAVLAVARVPG"
     gene            complement(5979..7298)
                     /gene="hflX"
                     /locus_tag="VF097_08620"
     CDS             complement(5979..7298)
                     /gene="hflX"
                     /locus_tag="VF097_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013474486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX6262894.1"
                     /translation="MTERAPRGRRVSESPLSARWRPTTSEPAVLVGIAPDEGDLDELA
                     ALADTAGAEVVGRLVQPRDAPDPRTFIGKGRLGDLHALVHDTGAGLVIFDQELTPGQL
                     RHLEEDLGVKVVDRTALILDIFALHARSREGKLQVELAQLSYLLPRLRGWGEAMSRMG
                     GGIGTRRGPGETKMEVDRRRIRQRISRLRRELRDVARSRDVTRARRERSRTPQIAIAG
                     YTNAGKSTLMNALTGADALVADQLFATLDPTVRRLRLPGGRGVTLSDTVGFVGKLPHD
                     LVEAFRSTLEEVTRADLILHVANASAADVDEQVAAVREVLAEIDAAQLPEALALNKAD
                     LVSAEDRARLERRFPDAVLVSALEGDGLDKLLARVDDVLPSPPVEVHLRIPYARQDVT
                     ARLHREAEVLSVEPGEQDTLVDVRLRQDQMSWIEEFLVRSPRVRRSD"
     gene            complement(7295..8449)
                     /locus_tag="VF097_08625"
     CDS             complement(7295..8449)
                     /locus_tag="VF097_08625"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HEX6262895.1"
                     /translation="MRIASRLDRLPPYMFAELDRKIDEKREAGVDVISLGVGDPDTPT
                     PEHVVEALREAATDPATHRYPSYFGMPELRRAIAGHYRERFGVELDPDAEVQPLIGSK
                     EGLAHLPIAFVDPGDEALVPDPGYPVYASSTTLAGGTPVAFPLTADRGFLPDLEAMPA
                     GDRTRILWLNYPSNPTAAVADLPFLERAVAFALEHGLLLVHDAAYSEMTYEGYVAPSV
                     LEVPGAKDVAIEFGSVSKTHNMTGWRVGWAVGNPQAVRGLAQVKTNIDSGIFDAVQRA
                     AIAAITGPQDHLERLRALYQKRRDVVIATLNDLGWDLEPPKGSFYIWFPTKDGMGSAE
                     FCDLVLERTGVVISPGSGYGAGGEGYARISLTVADDRLQEAMDRFREALS"
     gene            complement(8446..9336)
                     /gene="dapF"
                     /locus_tag="VF097_08630"
     CDS             complement(8446..9336)
                     /gene="dapF"
                     /locus_tag="VF097_08630"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX6262896.1"
                     /translation="MKVSKYHGTGNDFVMWVDLDDRHPQTPELVAALCDRHLGVGGDG
                     TIRVTGSDRADFFMDYWNASGETAEMCGNGIRCLAKLVYDRGLTTATEIDVDTRAGVK
                     HLVLTVEDGVVEAVRVDMGPPAFERSALPMRGDGTFLDQPFEVEGTTYRAFGISMGNP
                     HLVLIGDRDPREIEVRRIGPALEHHEDFPERTNVEFVRVVDGVIEVRVWERGSGETMA
                     CGTGACAALVGANLAGLAPRRSPVRFPGGVLDLEWAEDDRVFLRGPATFVFDAELSPV
                     WVERARAGRVVAPTPEGVAT"
     gene            complement(9333..10310)
                     /gene="miaA"
                     /locus_tag="VF097_08635"
     CDS             complement(9333..10310)
                     /gene="miaA"
                     /locus_tag="VF097_08635"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX6262897.1"
                     /translation="MRTARAARAAAATAADLSIPRALVGPTASGKTEASIELARRLDA
                     EVILVDSMTVYRGMDVGTAKPGPDERARIPHHLADVADPTESFSVARFQALAREAVRE
                     IAARGRVPLLVGGSGLYFRAAVDDLEFPGTDPGLRGLLNREAGLLGPEWMHARLADLD
                     PDAAAKIDPENVRRSVRALEVAALTGRPFSSFAAAWERYPEGRVVAAGLRAASDVLVA
                     RIEVRVEAMLEGGLVDEVRVLLDRGFGPFLTSSQAIGYLEVVRHLEGRLSLEEAAAEI
                     ARRTRALARRQMAWFRRDPRIRWFDVDRGGPPMDEIEDHLRGAAPGRER"
     gene            complement(10346..11638)
                     /gene="miaB"
                     /locus_tag="VF097_08640"
                     /pseudo
     CDS             complement(10346..11638)
                     /gene="miaB"
                     /locus_tag="VF097_08640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
     gene            complement(11649..>13167)
                     /gene="rny"
                     /locus_tag="VF097_08645"
     CDS             complement(11649..>13167)
                     /gene="rny"
                     /locus_tag="VF097_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017594580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX6262898.1"
                     /translation="AALVALVVGLILGFLLRAALRGRAVSAAESRARRLVLEAEEEAQ
                     TTVRRSLDEVKDEIAAMRREAEEEIRLRREEAARQEERQAGREEALESKAARIEQAQA
                     ALERDRATLAAKADELKAAGEDIQKRLERVASMTAQEAKEALAEQMRDAAKREAMANI
                     REAEERAREEAEERARKIVTIAIQRVAAEQTADASVAVFPLPSDDLKGRIIGREGRNI
                     RHFEALTGVNLVIDDTPEAVVLSSFDPVRREVARLTLEKLVQDGRIHPARIEEMYERS
                     SGEVEEQVRRAGEQAVIDVGLTDVHPEMIRVLGRLQFRTSYGQNVLRHLVESAHIAGV
                     MAAELGLDPTLVKRAALMHDIGKAVTHEVEGSHALIGAELARRLKEPPEVVHAIESHH
                     GEVEQRTVIAVLAQTADQISGGRPGARRESIEQYVKRLERLEEICSAHPGVEKVYAMQ
                     AGREVRVMVQPEEVDDLAAQVLARDIAKQVEEELQYPGQIKITVVRETRASELAK"
BASE COUNT         1814 a         4816 c         4546 g         1991 t
ORIGIN      
        1 gaggaccagg gcgtcgagga tctcacgatc cccgacgcct gatcgcgctc aggagcagcc
       61 gctggtcgcg ccgcaggagt tgcagacgta gcaggagccg gccggctgca tccgcgaccc
      121 gcacgagtag cagagcgggg cgtccttcgc cttggcctcc gccgtgccct gcttctcgtc
      181 gagctccacg ctgacgtcca ccacctgcgg ggccggatcg gctcccgccg cccccgcgtg
      241 ctcgctcgcg gtctccttcc gctcctcgat cgagtggacc ccgagcgcct tccgcgtctc
      301 cggcggcagg tactcgagcg ccagacgccg gaagatgtag tccaccagcg aggtggcgaa
      361 ccggatgtcc gcatcggagg tgatccccga cggctcgaag cgcatgttcg tgaacttctg
      421 cacgtacgcc tcgagcggca cgccgtactg caggcccagc gagaccgaga tcgcgaacgc
      481 gtccatgatc ccggccaggg tcgagccctg cttcgcgacc ttgaggaaga tctcgccgat
      541 gccgtcctcg ggatactcgc cggcgatgat gtagccgtcg gcctccccca ccgagaacga
      601 gaaggtcttc ccgggccgcg tggaggggag gcgccgccgc accggggtgc ccgcgggcgc
      661 cgtcgtcggc tcggtcgccg acttcttctt gtcggcggag agcggctggg ccaccttgca
      721 gttgtcgcgg tagatcgcca cggccttcag gccgagcctc cacccctcga ggaagagctg
      781 ctccacgtcc tcgacggttg cctcctccgg catgttcacc gtcttggaga tcgccccgga
      841 gatgaacggc tgggcggccg ccatcatccg gacgtgcccc atgtagtgga tcgcctgctc
      901 ccccatcgag gtgtcgaaga cgctctcgtg ctccggcctg aggtgcggcg cgcccttcac
      961 cgagttgtgc tcggcgatgt acccgacgat gtccttcgtc tggtcctcgg tgtagccgag
     1021 ggtctccagc gcgcggggca ccgtgcggtt gacgatctgc atcgtcccgc cgcccaccag
     1081 cttcttcgtc ttgacgaggg ccaggtcggg ctcgatgccg gtcgtgtcgc agtccatcat
     1141 cagcccgatc gtcccggtcg gcgccaacac gctggcctgc gcgttccggt acccgaactg
     1201 ctcgccgagc ccgatcgcct cgtcccaggc ctgcttcgcc gccgagagca gaccctcggg
     1261 gacctggtcg gcgtcgatct cgtcggcccc ggcccggtgc ttccgcatca cgcggagcat
     1321 ggcgtcgcgg ttgggcgcgt agccggcgaa cggacccatc ttctcggcga tccgagccga
     1381 ggtggcgtag gcgtgccccg tcatgatcgc ggtgatcgcg cccgcccacg cgcggccctg
     1441 gtcggagtcg tacgcaagcc cccgcgccat cagcaggccc ccgaggttcg cgtagcccag
     1501 gccgagctgg cggaaggcct tggcgttcct ctcgatcttc tcggtggggt agctggagtt
     1561 ccccacgagg atctcctgcg cggtgaacac gacctcgacc gcccgcttga acgcctggat
     1621 gtcgaagctc ccgtcgtggt tgaggaactt cagcaggttg aggctggcca ggttgcaggc
     1681 ggagttgtcc agatgcatgt actccgagca cgggttgctg gcgttgatcc gacccgtcgc
     1741 cgggcaggtg tgccagtcgt tgatcgtcgt gtcgtactgc atgcctggat cggcgcactc
     1801 ccatgccgcc tgcgcgatct gccgcatgac gtcgcgggca cgcaccgact tcacggtcga
     1861 gccgtcgagg acggccttga ggttccagtc gcggtcctcc aggtgcgcct tcatgaactc
     1921 gtcggtcacc cgcaccgagt tgttggcgtt ctggtactgg gtcgagtgcg agtcaacccc
     1981 gtcgaggtcc atgtcgaacc cgttctcgcg gagcacgcgg gccttccgct cctcgaccgc
     2041 cttgcaccag atgaattcct cgacgtcagc gtggtccacg tcgaggatga ccatcttcgc
     2101 ggcgcgccgc gtcttgccgc cggacttgat cgtgccggcg gaggcgtcgg cgccacgcat
     2161 gaacgacacc ggcccggagg cgagcccgcc cgaggacagg cgctccttgg acgaccggat
     2221 cgtggagaga ttgatgccgg acccggaccc gcccttgaag atcgtcccct cctcgacgta
     2281 ccagttgagg atcccggtca tcgtgtcctc caccgagagg atgaagcagg ccgagcactg
     2341 gggcttctcc tcggccccga cgttgaacca gaccggcgag ttgaacgcgg ccttctgcgt
     2401 cacgaggagg tggctcagct cctcggagaa gaccgtggcg tcgcgctcgg tggcgaagta
     2461 gccgtccttc tcgccccagc cccggatcgt gtccacgacc ctgccgatca gctggcggac
     2521 cgaggactcg cgctgaggag tgccgagcgg gccgcggaag tacttctggg ccacgatgtt
     2581 cgtggcgttc tgggaccagg acttggggaa ctccaccccg cgctgctcga aggcgttccc
     2641 gcccgtcttg aggttgggga tcaccgcgtc gcgggtctcc cactcgatct cgtcgaacgg
     2701 gtgcacgccc tcgctggtga agtagcgctc gaactcgagc ccctttgtct tgcgatcgag
     2761 gtccttggtc gccacggctg acgcctcctt ccggggtgcg aattacagtg gcgtgattct
     2821 acgctcgtcg gccggccctt gcaagagtgt acgaccgatg atgttgtggc gtctacccat
     2881 gaggaccaca agatgttgtg gtttctgtgg agaacctgtg gaaagcgtgt ggagggtcga
     2941 tcacccctcc ttggggcttc tcttctgcag caggagcccc agctcgcgct cgaagtcggt
     3001 gagctcctgg aagtccttgt agaccgaggc gaagcggatg taggcgatct cgtcgagctt
     3061 ccggagctgg tcgagcacct ccacgcccac ctcctgggtg gtgacgaccg ggcccttgcg
     3121 ctccagccgc cgctccacct cgtccaccag ccgcaagacc tgcgtctcgt ccaccggcct
     3181 gttcttgatc gccttcatca cccccgagac caccttggag cggtcgtagg gctccttgcg
     3241 gccgtcgagc ttcaccacgg tcaggccggc cccgtcggcc cgctcgtagg tggtgaacct
     3301 ccggccgcac gaggggcacg cgcggcgacg gcggatcgaa cggccgtcct cggcggctcg
     3361 agagtccacg acccgggtgt cgtcggcccc gcagtagcgg catcgcatgc acaacatctt
     3421 ggaccgtcgg ggagaggacc tcaagatgtg gtagcggccc tcccctgcga taattcggtc
     3481 atgccccagg actgggagct cgcgctccgg ctcctcgccg ccgccgggct gggcggcctg
     3541 atcgggttcg agcgggagat ccgggaccag cctgccggct tccgcaccca catcctcgtg
     3601 gccgtcggct cgtgcctctt cgccatcgtc tcggcctacg gcttcgacgc gttcctcggg
     3661 ttccggcccc aggagatccg cttcgacccc tcgagggtgg ccgcccagat cgtgaccggg
     3721 atcggcttcc tcggggcggg ggcgatcatc cgatacggca tgaccgttcg cggcctgacc
     3781 acggccgcca gcctgtggat cgtggccgcg atcggtctcg ccgtcgcggt cgggcagtgg
     3841 atcaccgcag gcacggccac cgcgatcacg ctggtcgcgc tctgggcgct ccgccacatc
     3901 cgccccaggc tcacccgagg tctcaagccc gagcacgagg agttcttcct cgacctggag
     3961 ccgacgacct cgctggagtc ggtcgtgcgt cacgtccacg aggcgggcct ccgcatcgac
     4021 cacctccaga tcgaggaggg gggcgagggc gagcgcgccg tctccctcgt cctgcggctc
     4081 ccgccggatc gcagccccga ggacgtggcc gcgctgctca cggaggctcc ggggatccgg
     4141 aacgtggggt ggacccgcta gggggcgggg agggccagcc ggaggccggg cacgaggacg
     4201 tcgcccgggc ccaggccgtt caggtcgcgg aggtcctgca cgagcgggcg ggggtcgccc
     4261 tccggtccca ccaggcccct ggcgatgccc cacagggtgt ccccccgggc cacgacgtgg
     4321 acgtccccgc ccgatcctcc cgcgggcgcc tcgatccggc cgctggcagc ccctgagatc
     4381 aggaccaccg tcacggagac cgccgcgatc agcccggcga tcctccccgt tcgaacccgt
     4441 gttcgaccca tgttggcagt atgccgaaca cccgttcgag cgtcaagcac tcgaacaggt
     4501 gtttgcctgc ggcccctccg cgtgctagcg tcgaccggac caagaggagg tcggcatgga
     4561 cgacatcacg ccacggcagc agcggatcct cgacttcatc cgggccacgg tgcgggatcg
     4621 agggtacccg ccgaccgtgc gggagatcgg ggaggcggtc ggcctcacct cgtcctccag
     4681 cgtgcacgcc cagctcgcga acctccagcg gaagggtctg ctccgccgcg acccctccaa
     4741 gccgaggacg atcgagctcg ccgagcaccg cgccgcgagc gcgacctcgg tccccctgct
     4801 gggccgcgtg gcggcgggcg cgccgatcct ggccgacgag aacctcgagg accacatcgc
     4861 ggtgcccacc gacttcgccg gcggcgggga gctgttcgcc ctgcgcgtgg agggcgactc
     4921 gatggtggaa gccgggatcc tcgacggcga cgtggtggtc gtgcggcgcc aggacgtcgc
     4981 cgcggacggc aacatcgtgg ccgcgctcgt ccccgggccc gcggagcacg aggccacggt
     5041 gaagcgtctc cggcgcggcg ggggcgggct cacgctcgtc ccggagaacc cggccttgga
     5101 gcccttcccc atgccagagg gcgggcgcgt gctgggggtc gtcaccgccg tcctgcggaa
     5161 ggtctgacta gcccgggacg cgggccaccg ccaggaccgc ctccgaccga tcgacgaagg
     5221 ctctgtccgg gagcgcgtcg agacgcttcc cgaccgcggc caggcacgcc gcccgggcca
     5281 cctcatcgag ggaccccaga cgctcccggg aggtggagag gcgcacccgc tggccccaga
     5341 actcatcgcg cgagagccgc aggttcgctc gcgccgtctc gacggacagc atctcgagcc
     5401 cggccgcctc cacgaggcca cggactttct cgggggcgtc cgtccgctcg tgggccgaga
     5461 ggctcggcgt gggagcggcc ccgtgcgcgt ccagctcctc ctcccacgcc gccaggggtg
     5521 ggcacagatc gtggtccgtc cacgtcgcca cccccaccga ccccccgggg ccgagcaccc
     5581 ggcgagcctc gcgcaggccg gcgacggggt cggggaggtg gaacagcatg aaggccatca
     5641 cgaccacatc gaacgtcccg gcggcgaacg ggaggcgctg ggcgtcggcc acgaccctcg
     5701 ggatcgaggg gtcggcctcc ctcagcatgg cctcggtgac gtccgcggcc acgacgtccg
     5761 cccggggagc gagaccgcgc agctcgggca gcagggcgcc cgttccggct ccgaggtcga
     5821 gcacgcggcg ggcggcgccg agcgggagcc ggcgggcgag gcgggcgctg agcgtgacca
     5881 ggacgggggc ccagcagtcg cggtaggcgc tggggtccgc cgtgtaccgt tcgacgaccc
     5941 tccggaccca ctcctggtcg ggaaggtcca tccggccctc agtcggagcg gcgcacgcgc
     6001 ggcgaccgta ccaggaactc ctcgatccag ctcatctggt cctggcggag acggacgtcc
     6061 acgagggtgt cttgctcccc cggctcgacg ctcagcacct cggcctcacg gtgcagccgg
     6121 gccgtcacgt cctggcgggc gtacgggatc cgcaggtgca cctcgaccgg cggggacggg
     6181 aggacgtcgt ccacccgggc caacagcttg tccaggccgt cgccctcgag cgcagacacg
     6241 agcaccgcgt cggggaagcg ccgctcgagc cgggcccggt cctcggcgga gacgagatcg
     6301 gccttgttga gagcgagagc ctcgggcagc tgggcggcgt cgatctcggc caggacctcg
     6361 cgcacggcgg cgacctgctc gtcgacgtcg gcggcggacg cgttcgccac gtggaggatc
     6421 aggtcggccc tcgtcacctc ctcgagggtg gagcggaagg cctccacgag gtcgtgcggg
     6481 agcttcccca cgaaccccac ggtgtccgag agggtgacgc cgcggccgcc ggggagccgg
     6541 agcctccgca ccgtcgggtc gagcgtggcg aagagctgat ctgcgacgag cgcgtccgca
     6601 ccggtgagcg cgttcatcag cgtggacttc cccgcgttcg tgtacccggc gatcgcgatc
     6661 tgcggggtcc gggagcgctc ccggcgagcc ctggtcacgt cgcgagagcg ggccacgtcg
     6721 cgcagctcgc gacggagccg ggagatccgc tgacggatgc gccggcggtc gacctccatc
     6781 ttcgtctcgc ccgggccacg acgcgtcccg atgccgccgc ccatcctcga catcgcctcg
     6841 ccccaaccgc gcaggcgggg gaggaggtag ctgagctggg ccagctcgac ctggagcttg
     6901 ccctccctgg agcgggcgtg gagcgcgaag atgtccagga tcagcgcggt gcggtcgacg
     6961 accttcactc cgaggtcctc ctccaggtgg cgcagctgcc ccggcgtcag ctcctggtcg
     7021 aagatcacga gccccgcgcc ggtgtcgtgg acgagagcat ggaggtcgcc gagcctcccc
     7081 ttcccgatga aggtcctcgg atcgggggcg tcgcggggct gcaccaggcg gccgacgacc
     7141 tccgcgcccg cggtgtcggc cagggcggcg agctcgtcga ggtcgccctc gtccggagcg
     7201 atgcccacga ggaccgcggg ctcggaggtc gtcggccgcc accgggccga gagcggggac
     7261 tcggagaccc tccgtcccct cggagcgcgc tccgtcacga gagcgcctcc cggaagcggt
     7321 ccatcgcctc ctgcagccgg tcgtcggcga ccgtcagcga gatccgcgcg tagccctctc
     7381 ccccggcccc gtagccgctc cccggcgaga tcaccacgcc ggtccgctcc agcacgaggt
     7441 cgcagaactc agccgacccc atcccgtcct tcgtggggaa ccagatgtag aaggacccct
     7501 tcggcggctc gaggtcccac ccgaggtcgt tgagcgtggc gatcaccacg tcccgtcgct
     7561 tctgatacag ggcccggaga cgctccaggt ggtcctgcgg gccggtgatg gccgcgatcg
     7621 cggcccgctg caccgcgtcg aagatccccg agtcgatgtt cgtcttgacc tgggccaggc
     7681 cccggacggc ctgcggattc ccgacggccc accccacgcg ccagcccgtc atgttgtggg
     7741 tcttggagac cgagccgaac tcgatcgcca cgtccttcgc cccgggaacc tccaggaccg
     7801 agggggccac gtagccctcg taggtcatct ccgagtaggc ggcgtcgtgg acgagcagga
     7861 ggccgtgctc cagggcgaag gccacggccc gctcgaggaa ggggagatcg gccaccgccg
     7921 ccgtcgggtt cgaggggtag ttcagccaca ggatccgggt ccggtccccc gccggcatcg
     7981 cctcgaggtc cggcaggaag ccgcggtccg cggtcagggg gaaggccacc ggcgtccccc
     8041 cggccagcgt ggtgctcgac gcgtacacgg ggtagccggg gtcggggacg agcgcctcgt
     8101 ctcccgggtc gacgaacgcg atcgggaggt gggccagccc ctccttcgac ccgatcagcg
     8161 gttgcacctc ggcgtccgga tcgagctcca cgccgaaccg ctcccgatag tggccggcga
     8221 tcgctcgccg cagctccggc atcccgaagt acgaggggta gcggtgggtg gcgggatcgg
     8281 tggcggcctc ccgcagggcc tccacgacgt gctcgggggt gggggtgtcc gggtcgccga
     8341 cgcccagcga gatcacgtcc acgccggcct cgcgcttctc gtcgatcttc cgatccagct
     8401 cggcgaacat gtacggcggc aggcgatcga ggcgcgaggc gatcctcacg tggccacccc
     8461 ctcgggcgtc ggggccacga cgcgccccgc ccgagctcgt tccacccaca ccggagagag
     8521 ctcggcgtcg aagacgaacg tggccgggcc cctgaggaac acgcggtcgt cctcggccca
     8581 ttcgagatcg agcactccgc ccgggaagcg gacgggcgag cgccgcgggg ccaaccccgc
     8641 gaggttggcc ccgacgagcg cggcgcaggc cccggtgccg caggccatcg tctcccccga
     8701 cccgcgctcc cagacccgga cctcgatcac gccgtcaacg acccggacga actccacgtt
     8761 cgtccgctcc gggaagtcct cgtggtgctc cagggcgggg ccgatcctcc gcacctcgat
     8821 ctccctgggg tctcgatcgc cgatcaggac gaggtggggg ttgcccatcg agatcccgaa
     8881 ggcccgatag gtcgtcccct cgacctcgaa gggctgatcg aggaaggtcc cgtcgccccg
     8941 catggggagc gcggaccgct cgaacgccgg ggggcccatg tccacgcgga cggcttcgac
     9001 cacgccgtcc tccacggtga ggaccagatg cttcacgccc gcgcgggtgt ccacgtcgat
     9061 ctcggtggcc gtggtcagcc cgcggtcgta gacgagcttg gccaggcacc ggatcccgtt
     9121 cccgcacatc tcggccgtct cgccgctcgc gttccagtag tccatgaaga agtcggcccg
     9181 gtccgagccc gtgacgcgga tcgtcccgtc gcctcccacc cccaggtggc ggtcgcagag
     9241 cgccgccacg agctccggcg tctgcgggtg gcgatcgtcg agatcgaccc acatcacgaa
     9301 gtcgttcccg gtgccgtggt acttcgatac cttcatcgtt ccctccccgg ggccgcgccc
     9361 cgcaggtgat cctcgatctc atccatcggc ggcccgcccc ggtccacgtc gaaccagcgg
     9421 atgcgcgggt cgcgacggaa ccacgccatc tgtcgtcggg ccagggccct ggtgcgccgg
     9481 gcgatctccg cggccgcctc ctccagagag aggcggccct cgaggtgccg cacgacctca
     9541 aggtacccga tggcctgcga cgaggtgagg aagggaccga acccccggtc gagcaggacg
     9601 cggacctcgt ccacgaggcc tccctcgagc atggcttcca ctcggacctc gatcctggcg
     9661 accaggacgt ccgaggctgc gcggagcccg gcggcgacca cgcgtccctc cggatagcgt
     9721 tcccacgccg cggcgaacga ggagaagggt cgtcccgtca gggcggccac ctcgagggcg
     9781 cggacgctgc ggcgcacgtt ctctgggtcg atcttggccg ccgcgtcggg atccaggtcg
     9841 gccagccggg cgtgcatcca ctccgggccc agcaggcccg cctccctgtt caggagaccc
     9901 ctgagcccgg gatcggtgcc cgggaactcg agatcgtcga cggccgcccg gaagtagagc
     9961 ccggaccctc ccacgaggag cgggacgcga ccccgcgccg cgatctcgcg gacggcctcg
    10021 cgggccagcg cctggaacct cgcaacgctg aaggactcgg tgggatcggc cacgtccgcg
    10081 aggtggtgag ggatccgcgc gcgctcgtcg ggccccggct tggccgtgcc cacgtccatg
    10141 ccgcggtaga ccgtcatcga gtcgacgagg atcacctcgg cgtccaggcg ccgggccagc
    10201 tcgatgctcg cctcggtctt ccccgaggcc gtgggcccga cgagcgcgcg cgggatcgac
    10261 aggtcggcgg cggtggccgc cgctgccctc gcggcgcgcg cggtcctcat cgcgaggcgg
    10321 ccacccgggc gggccggccg ctgaggtgat gcgggtgccc ccgggtgatc cacacgtcga
    10381 ggaaggagcc ggcctgagcg tcgccctccc agttgacgac gcggttcgtc cgggtccgcc
    10441 cctggacccc gcccttgcgg cccgagccct ccacgagcac ctcctggacg gagccctcga
    10501 ggccgcggtt cgcggccagc gagatccctt cctggagcgc aacgagacgg tcgaagcgct
    10561 cctggaggac ctccttgggc acctgcccct cgaacccggc ggccttggtg cccggccgcg
    10621 gcgaatactg aaacgtgtag gccgcgtcga atcgagcctc ctcgaccacg cggagggtct
    10681 cccggaagtc ctcctcggtc tcgcccggga accccacgat gatgtccgtg cttaccccga
    10741 tccccggcac cgcctcgcgg atccgctcca gccacgagag gtagcgggtc gagcggtagg
    10801 agcgtcgcat cgccttgagg atgcggtccg accccgactg cagggggaag tggatgtgct
    10861 cgcagacctt ctcgcactcg gccatggcct cgatcacgtc gggcgtgaag tcgtgggggt
    10921 gagggctcgt gaagcggatc cgctcgatcc cctccacctc gttcacggcg cgcagcaggc
    10981 gggcgaacag tggcttccgg ggggagtccg ccgccgtgag gtcccggccg taggtattga
    11041 cgttctggcc caggagggtg acctcgagga ccccctcggc ggccagcccc tggacctcgg
    11101 caagcacctc gcccagcggc ctggagcgct gggggccgcg gacgagcggc acgatgcaga
    11161 acgagcacag gttgtcgcag cccgtggaga cggagaccca cgcggcgtga ggcacctctc
    11221 tgcgggccgg aagcgcgctg gggaagacct ccgtgtactc gcggacgtcc atctggggac
    11281 cttcgcggtc cgcgcgctcc aggaggtcgg ccaggtacgg aacggcgtgg gtacccacga
    11341 cgacgtcgac ccacggggct cgccgctgga tctcgcctcg gtccttctgg gccaggcagc
    11401 ccgccaccac gatccgaaga cgggggttgg cctccttcgc ggcccggagc tggcccaggt
    11461 tcccgtagag ccggttgtcg gcgttctcgc ggacggcaca cgtgttgagg acgacgacct
    11521 cggcctcgcc gacgcgctcg gtccgctcga ggccgcgcga ctccaagagg cccgagatcc
    11581 gctcggagtc gtgctcgttc atctgacagc cgaacgtccg gatcaggtac cgctccatga
    11641 cgtccaggct acttcgccag ctcgctggcg cgggtctcac ggaccacggt gatcttgatc
    11701 tgacccgggt actggagctc ctcctcgacc tgcttggcga tgtccctggc caggacctgg
    11761 gcggcgaggt cgtccacctc ctcgggctgg accatcacgc ggacctcccg gcccgcctgc
    11821 atcgcgtaga ccttctccac gccggggtgg gccgagcaga tctcctccag ccgctccagg
    11881 cgcttcacgt actgctcgat gctctcgcga cgggcgcccg ggcgaccgcc ggagatctga
    11941 tcggcggtct gcgcgaggac ggcgatcacg gtccgctgct cgacctcgcc gtggtgcgac
    12001 tcgatcgcgt ggacgacctc ggggggctcc ttgagccggc gggccagctc ggcgccgatg
    12061 agcgcgtgcg agccctccac ctcgtgggtg acggccttcc cgatgtcgtg catcagcgca
    12121 gcccgcttga cgagcgtggg atcgagcccg agctcggcgg ccatcacccc cgcgatgtgg
    12181 gcagactcga cgaggtgccg gagcacgttc tggccgtagg aggtccggaa ctggagccgt
    12241 cccaggaccc ggatcatctc ggggtgcacg tcggtcagcc ccacgtcgat cacggcctgc
    12301 tccccggcgc gacggacctg ctcctcgacc tcccccgagg agcgctcgta catctcctcg
    12361 atcctcgcgg ggtggatgcg cccgtcctgc accagcttct ccagcgtgag gcgggccacc
    12421 tcccgacgca cgggatcgaa actcgagagc acgaccgcct cgggtgtgtc gtcgataacc
    12481 aggttcacgc ccgtgagggc ctcgaagtgg cggatgttcc tcccctcgcg gccgatgatg
    12541 cggcccttga ggtcgtcgga cggcagcggg aagaccgcca cggaggcgtc cgccgtctgc
    12601 tctgcggcca cccgctggat ggcgatcgtg acgatcttcc gggcccgctc ctccgcttcc
    12661 tcgcgcgccc gctcctccgc ctcccggatg ttggccatcg cctcccgctt ggccgcgtcg
    12721 cgcatctgct cggcgagggc ctccttggcc tcctgcgcgg tcatcgaggc cacccgctcc
    12781 agacgcttct ggatgtcctc gcccgcagcc ttcagctcgt cggccttggc cgccagcgtg
    12841 gcccggtccc gctccagagc ggcttgggcc tgctcgatcc gggcggcctt gctctccagg
    12901 gcctcctcgc ggccggcctg ccgctcctcc tgcctcgcgg cctcctcccg gcggagccgg
    12961 atctcctcct cagcctccct ccgcatcgcc gcgatctcgt ccttgacctc gtcaagcgag
    13021 cggcggaccg tcgtctgggc ctcctcctcc gcctcgagca cgagccgtcg ggcgcggctc
    13081 tcggcggcgg agaccgcacg gcctcgcagc gcggcgcgga ggaggaaccc gaggatcagg
    13141 cccacgacga gcgcgacgag ggctgca
//
LOCUS       DASUTZ010000197         2817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_454670, whole genome shotgun sequence.
ACCESSION   DASUTZ010000197 DASUTZ010000000
VERSION     DASUTZ010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454670"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..1117
                     /gene="cofD"
                     /locus_tag="VF097_08650"
     CDS             188..1117
                     /gene="cofD"
                     /locus_tag="VF097_08650"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019878263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEX6262899.1"
                     /translation="MRVAALAGGVGAGKLLRGLVRAVDPSDVTVIGNVGDDMVHLGLH
                     ISPDLDSVAYWLSGEMDRERGWGRAGETFRAMEELGRLGEQAWFSLGDRDLATHLHRT
                     QRLNEGASLSDVTAELCEALRVPCRVLPVTDDSVRTWIDVQGGSLPFQMYWVAHGAEP
                     KVERVRFEGAEKARPAAGALEAIAEADAIVICPSNPVVSVMPILAVPGVHEALRERRD
                     RVVGISPIVGGTVLQGMADKLMPAMGLEVSAAGAAEGYRGLLAAWVVDERDRDLAPKI
                     EEREGLRVGVTDTIMVDDEASERLASFALELLG"
     gene            1114..2391
                     /locus_tag="VF097_08655"
     CDS             1114..2391
                     /locus_tag="VF097_08655"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF009810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEX6262900.1"
                     /translation="MSVEVLPVAGLPEIGESDDLAALIADAAELRDGDVVAVTQKVVS
                     KAEGRLVPEGEDWVDREAQRVVARRGDLVIAATRHGFVCANAGIDHSNVPRGFLSLLP
                     EDPDASAAAITDGLLARTGRRVGVVVTDTFGRPWRHGVVNVAIGVAGLPALVDLRGEK
                     DAHGKVLEATVIALADEVAAAAGLVQGKADGVPVAVVRGLTSAAPPGRGRDLIRGRED
                     DLFRESPLQSIHARRTVRSFAEGTVPREVLREAAAASLTAPVPHGNRKPNRPWMWVVL
                     DSEPAKRRLLGAMAEAWVRDLRGDGLPEDRIRRRLDRSDALLGAAPVLAIPALSLANA
                     DAYPDQRRREAEREMFLLATGAMVQSYLLAIHAQGYGGAWVSSTLFCKEEAREALGLG
                     SEWLPMGSVACGPIEGDPPARPPIDPGSHLRFA"
     gene            complement(2371..2649)
                     /locus_tag="VF097_08660"
     CDS             complement(2371..2649)
                     /locus_tag="VF097_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262901.1"
                     /translation="MRGAAVAAFPVLVLGAAALIVAGLAGSGLSWVVVAPAAIVLLLG
                     AVTGAALAALAFSRREPWWGLALLVAWPVAVPLYAAALRRRRSGEAEV"
BASE COUNT          386 a         1008 c         1075 g          348 t
ORIGIN      
        1 cgggaggcga cggccagcag ccggtcgagg tcctcccccc gggccgcgag cagcgcggcc
       61 acgtctccgg gcgacaggga cttgcccgac tcggcgcggg ccagggcccg ccgaacctcc
      121 gcgggggtgg gagccatggc cggagcgtag cctcggccct cggttcggtc aaaggtaggc
      181 tgtgcgcatg cgggtggcgg cgctggccgg cggggtgggg gcgggcaagc tcctgcgagg
      241 gctcgtgcgg gccgtggacc cctcggacgt gaccgtgatc ggcaacgtcg gggacgacat
      301 ggtgcatctc gggctccaca tctcgcccga cctcgactcg gtggcgtact ggctgagcgg
      361 cgagatggac cgggagcgag gctgggggcg tgcaggggag acgttccgag cgatggagga
      421 gctggggcgg ctcggagagc aggcctggtt ctcgctcggg gaccgggatc tggctaccca
      481 tctccaccgg acccagcggc tgaacgaggg ggcctcgctc tcggacgtga cggcggagct
      541 ctgcgaggcc ctgcgcgtgc cctgtcgcgt gctgccagtc acggacgact ccgtgcggac
      601 ttggatcgac gtccaaggag gatcgcttcc cttccagatg tactgggtcg cgcacggagc
      661 cgagccgaag gtggagcgcg tgcggttcga gggagccgag aaggcgaggc cggcggcggg
      721 cgcgctggag gcgatcgccg aggccgacgc gatcgtgatc tgcccctcga accccgtggt
      781 gagcgtcatg ccgatcctgg ccgtgccggg ggtccacgag gcacttcgag agcggaggga
      841 ccgcgtcgtc gggatctcgc cgatcgtggg ggggaccgtg ctccagggga tggccgacaa
      901 gctgatgccc gcgatgggcc tcgaggtgtc ggcggccggc gcggcggaag gctaccgggg
      961 cctgctggcc gcctgggtgg tcgacgagcg ggaccgcgac ctggccccga agatcgagga
     1021 gcgggagggc ctccgcgtgg gcgtcaccga cacgatcatg gtggacgacg aggcgtcgga
     1081 gcggctcgcg agcttcgcgc tggagctcct gggatgagcg tggaggtcct cccggtcgcg
     1141 gggctccccg agatcggcga gagcgacgac ctcgcggccc tgatcgcgga cgcggccgag
     1201 ctgagggacg gggacgtggt ggccgtgacg cagaaggtcg tttcgaaggc cgaaggtcgg
     1261 ctcgtgcccg agggcgagga ctgggtggac cgagaggccc agcgggtggt ggcgcgtcgc
     1321 ggcgatctcg tgatcgccgc gacgcgccac gggttcgtct gcgcgaacgc cgggatcgac
     1381 cattcgaacg tcccccgggg cttcctcagc ctcctccccg aggaccccga cgccagcgcc
     1441 gctgcgatca cggacgggct cctggccagg acggggcggc gggtcggcgt cgtcgtcacc
     1501 gacacgttcg gccgcccctg gcgccacgga gtggtcaacg tggccatcgg cgtggccggg
     1561 ctccccgcgc tcgtcgacct gcggggcgag aaggacgccc acgggaaggt gctcgaggcc
     1621 accgtgatcg ccctggccga cgaggtcgcg gccgcggccg ggctcgtcca gggcaaggcc
     1681 gacggcgtcc cggtggcggt cgtgcgagga ctgacctccg ccgccccgcc cggccgcggc
     1741 cgcgacctga tccgcggacg ggaggatgat ctcttccgcg agtcccccct ccagtcgatc
     1801 cacgcccgcc gcacggtccg ctccttcgcc gagggcacgg ttccccggga ggtgctgcgg
     1861 gaagccgccg ccgcctcgct caccgccccg gtcccccacg gcaaccgcaa gcccaaccgc
     1921 ccctggatgt gggtggtcct cgactccgag cccgcgaagc ggcgcctgct gggcgcgatg
     1981 gccgaggctt gggtccgcga cctgcgtggg gacgggctcc ccgaggaccg gatccggcgg
     2041 cgccttgacc gctccgacgc cctcctcggc gccgcgcccg tgctggcgat ccccgccctc
     2101 tccttggcca acgccgacgc gtacccggac cagcgccgca gggaggccga gcgcgagatg
     2161 ttcctcctcg ccaccggggc gatggtccag agctacctgc tcgcgatcca tgcccaggga
     2221 tacggaggag cgtgggtctc ctccaccctc ttctgcaagg aggaggccag ggaggccctc
     2281 ggcctcggct ccgagtggct cccgatgggt tcggtggcct gcggcccgat cgagggggac
     2341 cccccggccc gtccgcccat cgaccccggc tcacacctcc gcttcgcctg agcggcggcg
     2401 acggagcgcc gcggcgtaca ggggcacggc gacgggccag gcgacgagca gggccaggcc
     2461 ccaccagggc tccctccgcg agaaggccag ggccgccagg gccgcgccgg tcaccgcccc
     2521 cagcaggagc acgatcgctg ccggtgccac aaccacccag ctcaacccgg agcccgccag
     2581 cccggccacg atgagcgccg cggccccgag cacgagcacc gggaacgccg ccacggccgc
     2641 ccccctcacc ccccgaacct ccgtgcgacg atgcctcgat ccttgcgtat agttgctcgt
     2701 cgagggatct ccccatggcg aacaagaaga agaagcggaa gaagctcgcg cccggcgcgc
     2761 ccacgtcggc ccccgccgag gagcgggagg cccggaagga ggccgctcgc caggacc
//
LOCUS       DASUTZ010000198        10498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1232197, whole genome shotgun sequence.
ACCESSION   DASUTZ010000198 DASUTZ010000000
VERSION     DASUTZ010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10498
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232197"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..689
                     /locus_tag="VF097_08665"
     CDS             249..689
                     /locus_tag="VF097_08665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX6262902.1"
                     /translation="MNLTLTRRGDYTVRAAIHLARRWGDGLAKLREISADMELPRSYT
                     PQVLGILVRAGLAEAKAGRDGGYQLVRDPASVSLLEVVEAADGPLRTTECTLRGGSCR
                     WDDACAVHPSWVAASEAFRGSLGSTSLADVAGVEPIVERRGAEG"
     gene            complement(693..1619)
                     /locus_tag="VF097_08670"
     CDS             complement(693..1619)
                     /locus_tag="VF097_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX6262903.1"
                     /translation="MDLESFARTLATTIREAVLPMFGHPDARRRAGTAAGGDPTFAID
                     EVAERTAEALVERRGDVAYFTEDAGLRTLGRPEGLLLLDPVDGSRPAVAGYETCGVSV
                     AVAPFGEGVTMADVRYGCVVEIATGTVFEARRGGGAVAEGAEIRPSGATEPRGMLWAG
                     GFRGQPVVPTVVALEEVFDVPDAEGAFFDQGSAAYSLTRLATGQLDAYVDVGQALVEE
                     IAGMEGRFREVGGGHILNTTTYDTAAGYLLLKELGLPVTDARGRPVGGVPLFAEDGAA
                     SLVSTVAACTPELHEALLEAVSRGIARLRSWG"
     gene            complement(1609..2703)
                     /locus_tag="VF097_08675"
     CDS             complement(1609..2703)
                     /locus_tag="VF097_08675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6262904.1"
                     /translation="MRIALACPYAWDAPGGVQVHVAQLAERLRGRGHRVLVVAPSRAR
                     IDDPHVVSIGGTIPVPVNGSIAPIAPRPAAARRTRHALDDFGPDVVHAHEPFVPSASM
                     FAVRWGGAPVVATFHAYADRSPLLAAAVPFLRPVWDRLTVRVAVSEAARRFVSRRFRG
                     EIRVVPNGVDVDLFRDARPADLPPGRRVLFVNRLDPRKGFAVMVRAFELLLGSHPDAL
                     LVVAGDGKERAAVSDLPRPARDRVVMLGNVPHDLLPPYHAACEVFAAPAVGRESFGIV
                     LVEAMAAGLPVVASDIPGYREVVRRGDDGLLVPPRDTAALAAALRRVLDDHELAERLR
                     DAGRARSAGYSWETVTRQVETAYEEAVGGP"
     gene            complement(2700..3653)
                     /locus_tag="VF097_08680"
     CDS             complement(2700..3653)
                     /locus_tag="VF097_08680"
                     /inference="COORDINATES: protein motif:HMM:NF005919.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HEX6262905.1"
                     /translation="MIERETFAETFSYWYYRSAEWAARALPERVGRRLFSAAAAVEHA
                     RNPAQRRVVARNLSRVLGKPPDSPEVRAAVREAFDSYGRYWYDTFLLRALPPEQVRKR
                     FDMRGLEFVDAALEQGRGALFCLPHLGNWDVAGHWMTLQGYALTAVAETLRPERLFRL
                     FLEHRRDLGMNIVPLTGTTKVGEQLLQLIAKNELIALVSDRDLRGRGVDVEMFGATRK
                     LPAGPALLSLTTGSPLLPSAVYDTPEGWLCYVTEPISVERSDVLRDDVTALTRTLAGR
                     FETFIAEAPTQWHMWQPAWPEDEVDAPRGSGLEPAPAEGPG"
     gene            complement(3650..4168)
                     /locus_tag="VF097_08685"
     CDS             complement(3650..4168)
                     /locus_tag="VF097_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262906.1"
                     /translation="MEQTIRPRSSSVWTILAGAVLAAASSAVFVLVEELQIAGAATLI
                     AVLGVVVAGWSARQSGEPRVGFVASMVERVVDAAILGALAWSLLPERAGAAALVALAG
                     SYVAAYLEVKAAGLGFEAGAWGLAWPLALVVLAAGLVAGEAELGLWGAAAISIAAALA
                     RSVQVARQREPS"
     gene            4272..6353
                     /gene="fusA"
                     /locus_tag="VF097_08690"
     CDS             4272..6353
                     /gene="fusA"
                     /locus_tag="VF097_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX6262907.1"
                     /translation="MNTYEPNAIRNVLLVGHGGTGKTTLAEALLFSAGATTRMGTVEE
                     GNTVTDFEPEEQKKQISVSLALAPFEWRGTKINLLDAPGYADFIGDVQAALRAADACL
                     FVVSAVDGVQVQHEVVWEMAVEAGLPRAVFVNKIDRERASFERTLDHLVQAFGTQVAP
                     LQFPIGEEHDFEGLADLLSGRAYRYQSGPKSEESDWPDDIAGKAEPMREKLAEAVAEG
                     DDALLEKYLEEGELSAEEITSGVKSGFLQARFVPVLVGSAASAKGVDRLADLIVNAFP
                     APTDRPPVKVMTQAGEEVEREADPNGPLTAFVFKSFSDPYVGQISLFRVLSGRIRPDS
                     TVHNATKGSDERIHQVFTLRGKEHENVSDVPAGDIAAVSKLSDTATGDTLTTKDAAAT
                     LPAVEWAEPLYAVAVHPKTQGDEDKLSTALHRIQDEDPTIRVERRVETGQTVVTGMGE
                     AHIDVLIERMQRKFGVEVTTEPAKVAFKETIRGRAEGLGRHVKQSGGHGQYGVCNIRV
                     EPLPRGEGFVFEDEIFGGAIPNQFIGSVEKGIVKAMQEGVLAGFPVVDVKVTLYDGKY
                     HSVDSSDMAFQIAGALAFRDATQNAGVVLLEPMVDVEVIVPEGMTGDIMGDLNSKRGK
                     VQGMDPTGTGKTRIRAQVPLTEMTRYPIDLRSITGGHGTFSMTFSHYEEVPSHIADRL
                     VQELKAADAED"
     gene            6360..6857
                     /locus_tag="VF097_08695"
     CDS             6360..6857
                     /locus_tag="VF097_08695"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018383424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX6262908.1"
                     /translation="MHETDEDLEALQRVLDESHAAGGGHLRSILQEDRRLAAEELPGL
                     MPGVQVIVLSTVTSDCRPIAGPVDGLFYRGAFHFGTSAGSVRFNHLGRRPQISAVHAR
                     GEELVVVVHGEARAIDVAKAEHEGFRAYLLEVYPDWESWWGEAPPPYFRIEPRKMFAS
                     RLPGA"
     gene            complement(6854..7570)
                     /locus_tag="VF097_08700"
     CDS             complement(6854..7570)
                     /locus_tag="VF097_08700"
                     /inference="COORDINATES: protein motif:HMM:NF013671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEX6262909.1"
                     /translation="MSEQPKDELLTITAQAREALLALRAQDPAPDSLGLRIAVAGERD
                     GRYLHDMSFELLEDASPEDVVLQHDDLPVIVPQDSMKMLRGAVVDLAPDGQSWTIDNP
                     NRPKRMLPLAPVGGVHAGHGDAGHEHAASPAAQAEIPEELRGTLQGDTAQQVIEVLDR
                     HINPSIAMHGGRAELAGLKDGVAYLRLSGGCQGCSMAAVTLRQGIERSLRQLVPEIEG
                     VADVTDHASGTDPFYSSSKS"
     gene            7590..8807
                     /locus_tag="VF097_08705"
     CDS             7590..8807
                     /locus_tag="VF097_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX6262910.1"
                     /translation="MARILLLLPATAYRADDFLEAAEALGVEVVVASDRRSALAAQME
                     DRALTVPMSDPDAAAKRIERFGREHQIDAVVPVDDRGVVVAAVAAQRLDLPHNPQGAA
                     TAATDKVRMRRALAAAGVPQPAFRVLGPEDAAGDLADELGYPVVLKPVSLSMSRGVLR
                     ADSPREARAAAGRIREILARAREDPLGPLLVERYVPGPEMALEGLLLRGDVETLALFD
                     KPEPMEGPTFEETIYVTPSREPFEMQDAVRTLVAGAAKAMGLREGPIHAEVRLGPSGP
                     VVLEVAARSIGGLCSRSLRFGAGISLEEVILRHALGLPTDDLHRESAASGVLMLPVPS
                     SGTLVEVRGQDQARGVPGIEGVTITIPPGGELVPLPEGDRYLGFVFARAETPDEVESA
                     LREARERLEPIVE"
     gene            complement(8808..9116)
                     /locus_tag="VF097_08710"
     CDS             complement(8808..9116)
                     /locus_tag="VF097_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6256 family protein"
                     /protein_id="HEX6262911.1"
                     /translation="MSRILREVVPPLLTIYVVVVAMLASLRRGWPPRRFERPLREVAV
                     TIMLGYGAFLLIVLVFMTWATGDESALPDAVWEGAALLGIAVPAFVALSAVDAFRRRR
                     "
     gene            complement(9113..9340)
                     /locus_tag="VF097_08715"
     CDS             complement(9113..9340)
                     /locus_tag="VF097_08715"
                     /inference="COORDINATES: protein motif:HMM:NF040017.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6186 family protein"
                     /protein_id="HEX6262912.1"
                     /translation="MTRLSATAAVLWGLVFAALLAVQGLGLVREGWPTLSDMLRAITR
                     HPVARWVLFGFWIWIGWHLFVRGWRFLLREP"
     gene            9439..10458
                     /locus_tag="VF097_08720"
     CDS             9439..10458
                     /locus_tag="VF097_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017835052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HEX6262913.1"
                     /translation="MTKTARAVSALVALSLMAPACGDGGAAEEQDVSLTVYSGRDEEF
                     IGPLYERFEDETGIDLEVRYGDSAELAATILEEGEASPADVFVSQDAGALGVVAREGL
                     LTEIDGEILDRVDERFRSPQGRWVGVSGRARVIAYNTETVSESALPSTVMDLTGPAYE
                     GRVGFPPTNASFQAFVAAMIVTEGEGATRDFLQGLADNEPRLFESNSDTVRAVAAGEV
                     DFGLVNHYYKYEVEAEDGPIPVENHFLSGGDPGALVNAAGAGVLASSDGPEASMRLLD
                     FLTSEEGQRYFATETFEYPVVPGIEPGPDLPPLAEIESPSIDLSDLGETLEPALRLLA
                     EVGLL"
BASE COUNT         1582 a         3775 c         3780 g         1361 t
ORIGIN      
        1 atctcaccgg tcccctcccc ctcgatccgg aacgccgagt cctgcgcggc gacggcgacg
       61 tcctcggacg agccgcccac gcccgccatc accgcgccga cgaccaggag ccacacgctc
      121 acggtcgcgg cgacccacat cctcaggaac cgcatatgta tccctcctca gggccaatcc
      181 ttgactccgt tcggcatgta gagtaccgta ctaaacgttc agtcgcaagg tcacggaagg
      241 gtccctggat gaatctcacg ctgacgaggc ggggcgacta cacggtccgg gcggcgatcc
      301 acctcgcccg acgctggggc gatggcctgg ccaagcttcg cgagatctcc gccgacatgg
      361 agctgccgcg cagctacacc ccgcaggtcc tggggatcct ggtgcgggcg gggctcgcgg
      421 aggcgaaggc gggacgcgac ggcgggtatc agctcgtccg cgatcccgcg tccgtcagcc
      481 tcctcgaggt ggtggaggcc gccgacgggc cgctcaggac gaccgaatgc accctgcgcg
      541 ggggctcgtg ccgctgggac gatgcgtgcg cggtgcaccc atcctgggtc gcggcatcgg
      601 aggccttccg cggatcgctg ggctccacca gcctggccga cgtggcgggc gtggagccca
      661 tcgtggagcg gcgcggcgct gaaggctgac ggtcagcccc aggagcgaag ccgagcgatc
      721 ccccgcgaca ccgcttccag cagggcctcg tgcagctcag gggtgcaggc cgcgaccgtt
      781 gacacgagcg acgccgcgcc gtcctccgcg aacaggggaa cccctcccac cggcctccca
      841 cgggcatccg tgaccgggag gccgagctcc ttcaggagga ggtagccggc cgcggtgtcg
      901 taggtcgtcg tgttgaggat gtgccctcca ccgacctccc ggaaccgccc ctccatcccc
      961 gcgatctcct ccacgagcgc ctggcccacg tccacgtagg cgtcgagctg accggtggcg
     1021 agccgggtca gcgagtaggc cgcgctcccc tggtcgaaga acgccccctc ggcgtccggc
     1081 acgtcgaaca cctcttcgag ggccacgacc gtggggacga cgggctggcc gcggaatccg
     1141 cccgcccaca gcatccccct cggctcggtc gcgcccgacg gccggatctc agctccctcg
     1201 gccaccgccc ctccaccccg gcgggcctcg aacaccgtcc cggtggcgat ctcgaccacg
     1261 cagccgtagc gcacgtcggc catcgtcacg ccctctccga acggcgccac cgccaccgag
     1321 accccgcagg tctcgtaccc ggccaccgcg ggacggctcc cgtcgacggg gtcgaggagc
     1381 aggaggccct cgggccggcc cagggtgcgg agcccggcgt cctccgtgaa gtacgcgacg
     1441 tctcctcgcc gttcgacgag cgcctcggcc gtccgctcgg ccacctcgtc gatcgcgaac
     1501 gtcggatcgc ccccggccgc cgtcccggcc ctgcgccgcg cgtcgggatg cccgaacatg
     1561 gggaggacgg cctcccggat cgtcgtggcc agggtgcggg cgaacgactc aaggtccacc
     1621 gacggcctcc tcgtaggcgg tctccacctg gcgcgtgacc gtctcccagg agtagccggc
     1681 cgagcgggct cgtccggcgt cgcggagccg ctccgcgagc tcatggtcgt ccaacacccg
     1741 ccgaagggcg gcggccaggg cggccgtgtc tcggggcggg acgaggagtc cgtcgtctcc
     1801 cctgcggacg acctcgcggt acccgggaat gtcgctcgcc acgaccggca gacccgccgc
     1861 catcgcctcg accagcacga tcccgaagct ctccctcccc acggcggggg cagcgaagac
     1921 ctcgcacgcg gcgtggtagg gcggtagcag gtcgtgaggc acgttcccca gcatcacgac
     1981 tcggtcccgg gcgggacggg gaaggtccga gaccgcggcc cgctccttcc cgtcccccgc
     2041 caccacgagg agcgcgtcgg ggtggctccc cagcaagagc tcgaacgccc ggaccatcac
     2101 ggcgaacccc ttccgtggat cgagccggtt gacgaacagc acccgacgac ccggcgggag
     2161 gtcggcgggc ctcgcgtccc tgaacaggtc cacgtccacg ccgttgggga cgacgcggat
     2221 ctccccccgg aaccgacggg agacgaagcg gcgggccgcc tccgacacgg cgacccgcac
     2281 ggtcaggcgg tcccagaccg gtcggaggaa cggaacggcc gcagcgagca gcggcgaccg
     2341 atccgcgtac gcgtggaacg tggcaaccac gggggcgccg ccccaccgga cggcgaacat
     2401 cgaggcgctg ggcacgaacg gctcgtgcgc gtgaacgacg tccggaccga agtcgtcgag
     2461 cgcatgcctc gtcctccggg ccgcagccgg ccggggcgcg atgggagcga tcgatccgtt
     2521 caccgggacg gggatcgtcc cgccgatcga gaccacgtgt ggatcgtcga ttcgcgcgcg
     2581 agacggcgcc accacgagga cgcgatgccc gcggccgcgg agccgctcgg ccagctgggc
     2641 cacgtggacc tgcaccccgc ccggcgcgtc ccacgcgtac gggcaggcca gggcgatcct
     2701 catcccggcc cttcggcggg ggccggctcg agcccggagc cgcgcggcgc gtccacctcg
     2761 tcctcgggcc acgccggctg ccacatgtgc cactgcgtcg gagcctcggc gatgaacgtc
     2821 tcgaagcgcc cggcgagcgt cctcgtgagg gcggtcacgt cgtcccggag gacgtccgac
     2881 cgctcgaccg agatgggctc cgtgacgtag cagagccagc cctcgggcgt gtcgtacacg
     2941 gcgctgggga gcagcggcga cccggtcgtc agcgagagca gcgcgggacc cgcgggcagc
     3001 ttccgcgtgg ccccgaacat ctcgacgtcg accccccgcc cccggaggtc ccggtcggag
     3061 acgagcgcga tcagctcgtt cttcgcgatg agctgcagca gctgctctcc caccttcgtg
     3121 gtgccggtca gcggcacgat gttcatcccg aggtcccggc gatgctcgag gaacaggcgg
     3181 aacaggcgct cgggccggag cgtctcggcc accgccgtca gcgcgtagcc ctgcagcgtc
     3241 atccagtgcc ccgcgacgtc ccagttcccg aggtgcggaa ggcagaagag cgcccccctc
     3301 ccctgctcga gggcggcgtc cacgaactcc agccctcgca tgtcgaagcg cttgcggacc
     3361 tgctcgggag gcagagcccg caggaggaag gtgtcgtacc agtagcgacc atacgagtcg
     3421 aacgcctcgc ggaccgctgc ccgcacctca gggctgtccg ggggcttgcc gagcacccga
     3481 gacaggttgc gcgcgacgac gcgccgctgg gcggggttgc gggcgtgctc gacggccgcc
     3541 gcggccgaga acagccgccg cccgacgcgc tcgggcagtg cccgggcggc ccactcggcg
     3601 ctgcggtagt accagtacga gaaggtctcg gcgaacgtct cccgctcgat cacgacggct
     3661 cccgctggcg ggcgacctgg accgacctgg ccagggcggc ggcgatcgag atcgccgccg
     3721 ccccccacag gccgagctcg gcctccccgg cgacaaggcc cgcggcgagg acgaccaacg
     3781 ccaggggcca cgccaagccc cacgcgcccg cctcgaaccc gagcccggcc gccttcacct
     3841 cgaggtacgc ggccacgtag gacccggcca gggccacgag cgccgcggcc ccggcccgct
     3901 ccggcagcag cgaccaggcc agcgctccca ggatggccgc gtccacgacc cgctctacca
     3961 tcgatgccac gaaccccacg cgaggctcgc ccgactgtcg ggcactccac ccggccacca
     4021 cgacgcccag cacggcgatc agcgtcgccg cgccggcgat ctggagctcc tcgacgagca
     4081 cgaagaccgc cgaggacgcc gccgccagga ccgcgccggc gaggatcgtc cacaccgacg
     4141 aggagcgggg tcggatggtc tgctccatgg gggatgcgag cgtagcacgg cgtccccggg
     4201 gccccggcgg ccagaacgcc ctctcgggac cccgcctata atccggggaa cgccgacccc
     4261 atgaggtgcc gatgaacacc tacgaaccga acgcgatccg caacgtcctg ctcgtcgggc
     4321 acgggggaac cgggaagacg accctcgccg aggccctcct cttctccgcc ggggccacca
     4381 cccggatggg cacggtggag gagggcaaca ccgtcaccga cttcgagccc gaggagcaga
     4441 agaagcagat cagcgtctcg ctggcgctgg ccccgttcga gtggaggggc acgaagatca
     4501 acctcctcga cgcccccggc tacgccgact tcatcgggga cgtgcaggcc gcgctccggg
     4561 cggccgacgc ctgcctcttc gtggtctcgg cggtggacgg cgtccaggtc cagcacgagg
     4621 tcgtctggga gatggccgta gaggcgggcc tgccccgggc cgtcttcgtc aacaagatcg
     4681 accgggagcg cgcctcgttc gagcggaccc tggaccacct ggtgcaggcg ttcggaaccc
     4741 aggtcgcccc cctccagttc cccatcggtg aggagcacga cttcgagggc ctcgcggatc
     4801 tgctgagcgg cagggcgtat cggtaccaga gcgggccgaa gagcgaggag agcgactggc
     4861 ccgacgacat cgcgggcaag gcggagccga tgcgcgagaa gctcgccgag gccgtggcgg
     4921 agggcgacga cgcgctgctg gagaagtacc tcgaggaggg cgagctcagc gccgaggaga
     4981 tcacctcggg cgtgaagagc gggttcctcc aggcgcgttt cgtgcccgtc cttgtcggct
     5041 cggcggcctc cgccaagggc gtcgaccgcc tcgccgacct catcgtgaac gccttccccg
     5101 cccccaccga ccggccgccc gtcaaggtga tgacgcaggc gggcgaggag gtggagcgcg
     5161 aggccgaccc gaacggcccg ctcaccgcct tcgtcttcaa gagcttctcg gacccgtacg
     5221 tgggccagat ctcgctcttc cgggtcctgt cgggccggat ccggcccgac tcgaccgtgc
     5281 acaacgcgac gaagggctcg gatgagcgga tccaccaggt cttcaccctg cgggggaagg
     5341 agcacgagaa cgtctccgac gtgccggccg gggacatcgc cgcggtgtcg aagctctctg
     5401 acacggcgac cggcgacacg ctgacgacga aggacgcggc ggcgacgctc cccgcggtgg
     5461 agtgggccga gccgctctac gcggtggccg tccacccgaa gacccagggc gacgaggaca
     5521 agctctccac cgcgctccac cggatccagg acgaggaccc cacgatccgc gtcgagcgtc
     5581 gcgtcgagac cggccagacc gtcgtcaccg ggatgggcga ggcccacatc gacgtgctga
     5641 tcgagcggat gcagcgaaag ttcggggtgg aggtgaccac cgagccggcc aaggtcgctt
     5701 tcaaggagac gatcaggggg agggccgagg gcctgggccg ccacgtgaag cagtccggcg
     5761 ggcacggcca gtacggcgtg tgcaacatcc gcgtcgagcc gcttcccagg ggcgagggct
     5821 tcgttttcga ggacgagatc ttcggcggcg cgatcccgaa ccagttcatc gggtcggtcg
     5881 agaagggcat cgtcaaggcg atgcaggaag gggtcctggc ggggttcccg gtcgtcgacg
     5941 tgaaggtcac cctctacgac ggcaagtatc actcggtcga ctcctccgac atggcgttcc
     6001 agatcgccgg ggcgctcgcg ttcagggacg cgacacagaa cgcgggggtg gtgctgctcg
     6061 agcccatggt ggacgtcgag gtgatcgtcc ccgaggggat gaccggcgac atcatgggcg
     6121 acctgaactc gaagcggggg aaggtccagg ggatggaccc aaccggcacc gggaagacga
     6181 ggatccgggc ccaggtcccg ctcaccgaga tgacccgcta ccccatcgat ctgcggtcga
     6241 tcaccggggg gcacgggacc ttctccatga cgttctcgca ctacgaggaa gtcccctcgc
     6301 acatcgcgga ccggctggtc caggagctga aggccgccga cgccgaggac taggcgagct
     6361 tgcacgagac ggatgaggac ctcgaggcgc tccagcgggt cctcgacgag agccacgccg
     6421 ccgggggcgg gcatctccgc agcatcctcc aggaggaccg gagactggcc gcggaggagc
     6481 tgccggggct gatgccgggc gtccaggtga tcgtgctgtc gacggtgacg tccgactgcc
     6541 ggccgatcgc gggcccggtg gacgggctct tctaccgggg agccttccac ttcgggacgt
     6601 cggcggggtc ggttcggttc aaccacctcg gcaggcgacc gcagatcagc gcggtgcacg
     6661 cgcggggcga ggagctcgtg gtcgtcgtcc acggcgaggc ccgggcgatc gacgtggcga
     6721 aggccgagca cgaggggttc cgcgcgtacc tgctcgaggt gtacccggac tgggagtcgt
     6781 ggtgggggga ggcgccgcct ccctacttcc ggatcgagcc gcggaagatg ttcgcctcgc
     6841 ggctccccgg cgcctagctc ttcgaggagc tgtagaaggg atctgtgccc gaggcgtggt
     6901 cggtgacgtc ggccacgccc tcgatctcgg ggacgagctg gcgcagcgag cgctcgatcc
     6961 cctgtcgcag cgtgacggcc gccatcgagc atccctggca ccccccggag agacggaggt
     7021 aggcgacgcc gtccttgagg ccggccagct ccgcgcggcc cccgtgcatc gcgatgctgg
     7081 ggttgatgtg gcggtcgagg acctcgatca cctgctgggc ggtgtccccc tggagcgtgc
     7141 cgcgcagctc ctccgggatc tcggcctggg ccgcggggct cgcggcatgc tcgtgcccag
     7201 cgtcgccgtg gccggcgtgc acgccaccca ccggggccag cggcagcatc cgcttgggcc
     7261 gattcgggtt gtcgatcgtc caggactgcc cgtcgggcgc gaggtccacg accgcgccgc
     7321 gaagcatctt catcgagtcc tgcgggacga tcacagggag gtcgtcgtgc tggaggacga
     7381 cgtcctcggg gctcgcgtcc tcgagcagct cgaacgacat gtcgtggagg tagcgcccgt
     7441 cccgctcccc cgccaccgcg atccgcaggc ccagcgagtc cggggcgggg tcctgggccc
     7501 gcagggcgag gagggcctcc cgagcctgcg cggtgatcgt gaggagctcg tccttcggct
     7561 gttcgctcat cactccctag gatacatgtg tggcccggat cctcttgctc ctgcccgcga
     7621 cggcctaccg cgcggatgac ttcctcgagg ccgcggaggc gctcggggtc gaggtcgtgg
     7681 tggcctccga ccgccgctcc gccctcgccg cgcagatgga ggatcgagcg ctgaccgtcc
     7741 cgatgtcgga cccggatgct gccgccaagc gcatcgagcg gttcggcagg gagcaccaga
     7801 tcgacgccgt ggtgccggtg gacgaccgtg gggtggtggt ggcggcggtg gccgcccagc
     7861 ggctcgacct cccccacaac ccgcaggggg cggccaccgc cgccaccgac aaggtcagga
     7921 tgcgccgggc cctggccgcg gccggcgtcc ctcagcccgc cttcagggtt ctgggcccgg
     7981 aggacgccgc gggggacctg gccgacgagc tcggctaccc ggtcgtcctc aagccggtct
     8041 cgctctcgat gagccgcggc gtcctccggg ccgacagccc acgggaggct cgggcggccg
     8101 ccgggcgcat ccgggagatc ctggcacggg cccgtgagga tcctctcggt cctcttctgg
     8161 tggagcggta cgtccccggc ccggagatgg cccttgaggg cctgctcctt cggggcgatg
     8221 tggagaccct ggccctgttc gacaagcccg agccgatgga ggggccgacc ttcgaggaga
     8281 cgatctacgt cacgccctcg cgagagccct tcgagatgca ggacgcggtg cggaccctcg
     8341 tggccggcgc ggcgaaggcg atggggctgc gcgagggccc gatccacgcc gaggtccggc
     8401 tcgggccgtc cggccccgtc gtgctcgagg tggcggcccg atcgatcggc ggcctctgct
     8461 cccgctccct ccgcttcggg gcgggcatct cgctcgagga ggtgatcctc cgccacgcgc
     8521 tcggcctccc gaccgacgac ctccaccgcg agtcggccgc ctcgggcgtc ctgatgctgc
     8581 ccgttccctc atccggcacg ctcgtcgagg tccgaggcca ggaccaggcg aggggcgttc
     8641 ccgggatcga gggggtgacg atcacgatcc cgcccggagg ggagctggtc ccgctgcccg
     8701 agggcgaccg gtacctcggc ttcgtcttcg cgcgggcgga gacgccggac gaggtcgagt
     8761 ccgccctgcg ggaggccagg gagcgcctcg agccgatcgt cgagtgatca gcggcgccgc
     8821 cggaaggcgt cgaccgccga cagcgccacg aacgccggga cagcgatccc cagcagggcg
     8881 gccccctccc acacggcgtc ggggagggcc gactcgtccc ccgtggccca cgtcatgaag
     8941 acgaggacga tcagcaggaa ggccccgtag ccgagcatga tcgtgaccgc gacctcgcgg
     9001 agcggacgct cgaaccgccg cggaggccag ccgcggcgga gggaggccag catcgccacg
     9061 acgacgacgt agatcgtgag gagtggaggg acgacctccc ggaggatccg gctcacggct
     9121 cgcggagcag gaaccgccag ccccggacga acaggtgcca cccgatccag atccagaagc
     9181 cgaagaggac ccacctggcg accgggtgcc tggtgatggc gcggagcatg tccgagaggg
     9241 tgggccaccc ttcgcgcacc aggcccaggc cctggacggc cagcagcgcc gcgaagacca
     9301 ggccccagag gacggccgcc gtggcgctca gccgcgtcat gcgggatagc atagcccttg
     9361 acgcatttcc gacccagtag gtaggatttc ggccgcccga aggtcgggtg ttagggatac
     9421 cttacgggag aacgatcgat gacgaagacc gctcgcgccg tctccgccct ggtggcgctg
     9481 tccctcatgg cccccgcctg cggggacggc ggagcggccg aggagcagga cgtctcgctg
     9541 accgtgtact cggggcgcga cgaggagttc atcgggccgc tgtacgagcg gttcgaggac
     9601 gagaccggga tcgacctcga ggtccgctac ggcgactccg cggagctggc cgccacgatc
     9661 ctggaggagg gggaggccag ccccgccgac gtgttcgtct cccaggacgc cggagccctg
     9721 ggggtcgtgg cgcgggaggg gctgctgacc gagatcgacg gcgagatctt ggaccgcgtg
     9781 gacgagcggt tccgctcccc ccagggccgc tgggtgggcg tctcgggccg agcccgggtg
     9841 atcgcctaca acaccgagac ggtgagcgag agcgcgctcc cgagcacggt gatggatcta
     9901 accggcccgg cgtacgaggg ccgcgtcggg ttccccccta cgaacgcgtc cttccaggcg
     9961 ttcgtcgcgg ccatgatcgt caccgagggc gagggcgcca cgcgcgactt cctccagggg
    10021 ctcgcggaca acgagccgag gctgttcgag tcgaactcgg acacggttcg ggccgtggcc
    10081 gcgggcgagg tggacttcgg gctcgtgaac cactactaca agtacgaggt ggaggcggag
    10141 gacgggccga tcccggtcga gaaccacttc ctttcgggag gggaccccgg cgctctggtg
    10201 aacgcggccg gcgccggcgt gctcgcctcc tccgacggcc ctgaggcctc gatgcgcctc
    10261 ctggacttcc tcacgagcga ggagggccag cggtacttcg ccacggagac gttcgagtat
    10321 ccggtggtgc ccgggatcga gccgggcccg gatctcccgc ccctggccga gatcgagtcg
    10381 ccctcgatcg acctctccga cctgggggag acgctggagc cggcgctgcg tctgctggcc
    10441 gaggtggggc tgctgtgagc gtgggggtgg cggcggccga gcccgctgcg cgggctcg
//
LOCUS       DASUTZ010000199        14765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1492285, whole genome shotgun sequence.
ACCESSION   DASUTZ010000199 DASUTZ010000000
VERSION     DASUTZ010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14765
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492285"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2066)
                     /locus_tag="VF097_08725"
     CDS             complement(<1..2066)
                     /locus_tag="VF097_08725"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX6262914.1"
                     /translation="MVVGVLEVVVATSFAALIFSGPLAEHLPAGIGLGLVAAAAILIT
                     VALVSSHRGTFGSVQDISAAVLSIVAASIVADLPPEDPRVFLTVVAAITLTSLLTGGF
                     FLLLGTFRLGDVIRFVPYPVVGGFLAGTGWLLFRGGMEVVSGVPLAFGRLGVLAESDV
                     LARWIPGVVFAVVLLVLARRSQHPMVIPLSVLGGIGAFYVVVLVAGAGIGAVEEAGWL
                     LGPFPEGGLWEPWVARSVAQADWSAVVGEAGNLATILIVSLLALLLNASGIELVVGRD
                     LDLNRELRAAGIANLAAGLGGGLPGFHALSLTALAYKMGARSRAAGVVAGGVCVVALV
                     AGGGILGMFPRPVLGGIIVFLGLAFLVEWVVDAWALLPRSEYLIVVLILVVVAAVGFL
                     PGVALGVLLAVVLFAVAYGRSDVVKHALSGATYHSKMDRPLAHREVLRRDGDRIHVLE
                     LQGFLFFGTANALLDRIRARATDGKLPPLRFLVLDFRRVIGLDSSVVLSFSKTQRLAA
                     SSGFAMVLTSMGDGVRRALEQGGLTEGDPSVRFFPDLDRGLQWCEDGLLDEEGAPEEP
                     PGTPWREALAGVGIPEDVLLGYMERIEAPAGEILIRQGDRTEDVLFLESGQVTTQVTR
                     ERGGPVRLQTLGPGAVVGEVALYAGGVRSATVVAERPSVLHRLTRDRLHEMEQRDPFA
                     AAALHRL"
     gene            complement(2254..2745)
                     /locus_tag="VF097_08730"
     CDS             complement(2254..2745)
                     /locus_tag="VF097_08730"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6262915.1"
                     /translation="MRLRNLTTEDLWLYEAIYRDPRMWEHLGGPHPKEGLAEKLERDA
                     AATEADEVWVLVIVLEEETAAGTVAIWPHELDGEVITEIGWMVLPDHQGKGVGSAGVR
                     MALDRAREKGRWDVLHAFPPVSNAASNAMCRTLGFTLVGARDYTARVTLRVNHWRLDL
                     RSA"
     gene            complement(2749..3072)
                     /locus_tag="VF097_08735"
     CDS             complement(2749..3072)
                     /locus_tag="VF097_08735"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX6262916.1"
                     /translation="MSARGHALPRPRLSRRAAGLAAISFVLLLALVLPLRQYVIQRSE
                     IAGLERKVQRLEEERATLEHRVERLQEPEYLERIARQCLGMVRPGEIAFVAVPEDGQS
                     LPASC"
     gene            complement(3069..4346)
                     /gene="eno"
                     /locus_tag="VF097_08740"
     CDS             complement(3069..4346)
                     /gene="eno"
                     /locus_tag="VF097_08740"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX6262917.1"
                     /translation="MPPIERIRAREILDSRGNPTVEAEVGLADGSLALAAVPSGASTG
                     EHEVLELRDGGDRYRGKGVLKAIENIHERIEPSLQGVEALDQREVDRMMIDLDGTLDK
                     SGLGANAILAVSLANAKAAAISSGLPLYRYLGGPNAHLLPVPLLNVINGGAHADNAIE
                     LQEFMLAPRGAASFSEAIRWGSEIFHTLKEQLHGKGLSTAVGDEGGFAPDLPGSAAAC
                     EALVGSIEAAGLEPGSDVAIALDAAASEIFRDGSYHLEGEARSAEQMAEYYDRLVTDF
                     PIVSIEDPLDENDWEGWAAVTSLLGNRIQLVGDDLLVTNPERLSRAIAEASCNAILVK
                     PNQIGSLTETLDVVDAAVASSFGVVISHRSGETEDSTIADVAVATNAGQIKAGAPSRG
                     ERTAKYNRLLRIEEELGDTARYAGAAVYRRFGR"
     gene            complement(4394..5080)
                     /locus_tag="VF097_08745"
     CDS             complement(4394..5080)
                     /locus_tag="VF097_08745"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6262918.1"
                     /translation="MARVDRATWIVELRRVNEEEEDVLAPTYDERWGEIDETHRAFVE
                     RFLSMVPPAGRVLDAACGTGKYLGMVLESGRSVLGVDHAGACLVEAKAKHPGAETERH
                     DLQDLAYREAFDGVMCVDAMEMIPPEDWPDVLRRFRRAIAGRGPLYLTVEIAPPEEVR
                     AGTEEGRRRGLPLADGEVIWEEYGYHHYPSLDRVRTWLREAGFAIEDEEEQPLEDEGF
                     AYHHVLARAV"
     gene            complement(5088..5984)
                     /locus_tag="VF097_08750"
     CDS             complement(5088..5984)
                     /locus_tag="VF097_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6262919.1"
                     /translation="MTHLLSLVGIVSISFSAIFVRLADVSPDTAAFFRAAYAVPALVL
                     VWLVFRDRDRRTTRERWMAFAAGLFLALDFAFWHRAIEDIGAGLATVIGNTQVVFVGI
                     YAWMILRERPTRLAFVLVPTVFAGVLLISGLGREDAFGSDPLAGVFWGVLTGLMYTAF
                     LVLLRASNRGLAPPAGPLMEATVGALIGMAIIGPVFDPSFSVAPSWPAHGWLLALALI
                     CQVFGWLLITVALPRLPSLETSVMLLLQPMLTVLWAYLLFSEILSLVQWLGVALVLIG
                     VGTLAARGSVEPEVERETALQQ"
     gene            complement(5981..6835)
                     /gene="mazG"
                     /locus_tag="VF097_08755"
     CDS             complement(5981..6835)
                     /gene="mazG"
                     /locus_tag="VF097_08755"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEX6262920.1"
                     /translation="MAEVHGTPDPLDHVPTGPVPSGRGGERLLDLVRVVARLRAPDGC
                     PWDRAQTHMSLRRHLLDEAYELIEAIEEGTPDDLKEELGDVLLQAIFHAQMAADDGTF
                     DVDDVAEVTVEKLIRRHPHVFAEVEADTPEAVYANWERIKAEEKGEHAVDEGVPKALP
                     ALIAAQKVQRRASGRGFDWAALAGAVEKVREELTELEEAAAAHAGRDVEGEAKDALEG
                     EVGDVLFAVTALARKLGVDAESALRSTIRKFVERFETMDERAREEGVRLEDLPEDDLL
                     RRFREARA"
     gene            complement(6841..7269)
                     /locus_tag="VF097_08760"
     CDS             complement(6841..7269)
                     /locus_tag="VF097_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262921.1"
                     /translation="MAVAAALVSGCGGDEAGTVPTPGPDEPTSTTAPSPTGPLTEGEP
                     QIVTLREGLVDVRPVTLERIERLGPRRLLAVFYGGVEECYGVARVDVEERSDAVTLTV
                     YEGRDPEAATCIEIAALKGIEVILGERVGDREVLDGTAVG"
     gene            complement(7305..8249)
                     /locus_tag="VF097_08765"
     CDS             complement(7305..8249)
                     /locus_tag="VF097_08765"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX6262922.1"
                     /translation="MRLRLLSLLAAVSLVLSGCAAFEPAAAVVGPHRIEDDEFLRLLD
                     FIMADPQFAEAAVANPDVERATLQRQLLTFLIQHEVVEERAREEGIQADRRRLEELMQ
                     DQAIQDQIERSDATVEDVRAFVRAQLIRSDVGQAVVEEEASDERLQATYEERAREFTE
                     VRASHILVSSQEEASRIVAEATRSNFARLARELSEDQGSGANGGDLGTRAAGAYVPEF
                     ADALLELPVGRIGGPVQTQFGFHVILVHSREAIPFEQAREQLLQELGPEVFRDWMLER
                     LRSLTVRVNPRYGTFDAETGEVVERRATTPLPGPQTTP"
     gene            complement(8260..11580)
                     /gene="mfd"
                     /locus_tag="VF097_08770"
     CDS             complement(8260..11580)
                     /gene="mfd"
                     /locus_tag="VF097_08770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX6262923.1"
                     /translation="MRKLLEPLIESEALQAVLARPGPVRVDPAGHAYAIAALAEALEG
                     PALAVTAGPRESAPLAHDVAAYLGADRAAHLPAWEALPVEGISPSPETSARRHAAIRR
                     AREARGAFVLVAPAVAVVQRLLPQVGAVDPLVVEAGQEIGPDELADRLVALGYTRSDL
                     VTHRGEFAVRGGIVDVFPGAAPRPVRIELFGDEVESLREFSASTQTSTGRLSRLEADP
                     VRELLPTPQVMERALEAVPRHKDRFRDAVDRVSQGLLFEGMEQAGPLIFEDLPLVRDR
                     LPGGVWAVVTAPRTAEERAESARRECEALADALGWAGVETMAAPGEALEGMAHVDLSD
                     FTEGVDLGIEGWGPSLGDPGRVASRAATLAGEGYTVAATAQGHGSLDRAVEVLSGAGV
                     SPDPAISDGPVTGFVFPEGRVAVVGEDDLFGRRRRTHEAPRLAGRSAGAFALELSPGD
                     FVVHRVHGVARYAGMTRRRIGDAERDYLLLEYAKGDRLFVPADQVDVVSKYVGGESPR
                     VHRLGTNDWVRTKARVKRALRDMAGELVRLYSARMAAKGHAFPPDDVWQHELETAFPY
                     EETPDQLTAIDEVKRDMEVARPMDRLLCGDVGYGKTEVALRAAFKAATSGKQVAVLVP
                     TTLLAEQHFVTFAERYAPFPVRVAMLSRFLSAKEQREVLDGLASGEIEVVIGTHRLLG
                     ADVRFKDLGLLIVDEEQRFGVAHKERLKQLRLDVDVLTMTATPIPRTLEMALTGIRDM
                     SVVDTPPEDRQPVLTYVGSWEDDVALGAARRELRRGGQVFWVHNDTRTIDRRAGWLAE
                     RVPEARIGVAHGQMDEKVLEKTMLAFWNGEVDVLLTTTIIESGLDVPTANTLVIERAD
                     ALGLAQLYQLRGRVGRSAERAFAYLFFPPQRQLTEEAHARLSAIGRHTALGSGFQIAM
                     RDLEIRGAGNLLGAEQSGHIAAVGFDTYARLLQEAVAELQGEPLPEEAPVRIDLPIKA
                     YLPPDYLGQESLRLDLYREIAGARDEADLDRIRSATEDRFGPLPPEGDALIELTRLRI
                     ACERHGVEEVSTFRGQVRVRPFVPDVEDLPERTAWHAATSTFNLDPPALAGADLARWL
                     RDRLVEASGARTEPAASG"
     gene            complement(11708..12889)
                     /locus_tag="VF097_08775"
     CDS             complement(11708..12889)
                     /locus_tag="VF097_08775"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6262924.1"
                     /translation="MTPAFLLIALSTLAYFVAEGALIPTVPRYVEGPLGGGESAVGIV
                     VGAFAVTALLFRPWAGRLADRRGRRFVMLVGMVVFAASVAGYVVAESIPLMVVMRLMT
                     GAGAAFFFVGAATAVTDLAPEARRGEAISFFSLSLYAGVGAGPVLGEVAVSSSGFDLA
                     WWLIVGASVLAFVLAVPVPDTRPPERSEGRMRIVHPAAVVPGLVILASIWGMAGFFAF
                     TPLYVERLGLEDSRFVFLLFSGVVLLIRGFGAKLPDRLGPGRAARAALTASALALLVI
                     GTFESTPGLLIGTAILGVGVGLAFPALMSMTIANTKPAERGAAVGTFSAFVDLAFGVG
                     PLTLGYVAEALGLRSVYFTAAGVALLGLVMLAVRRRRRLGPRRRRRRAEPEFPVPEIP
                     A"
     gene            12978..>14765
                     /locus_tag="VF097_08780"
     CDS             12978..>14765
                     /locus_tag="VF097_08780"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX6262925.1"
                     /translation="MQRILVGTDRSSSADRAVRWAADLASRFESELFVLQVILPEQET
                     DIEEGARAQHAFEELRLLAEEVAGARGKARVVVDDDPAAAICGAAAEEEVDAVVVGNF
                     GMAGRKRFLLGNIANRVSHSATCTVIIANTVSPEDRVVHPTREEREAAETEPHLLPRA
                     ARIGRVMAKAGLRDLFRPGASDQDLRDRAGKLRTAFEELGPTFGKLGQVLATRPDLLP
                     QPFIHELSSLHSSVPPVSEEEIVAVMEQELGVPWEDVFESIEPEPLAAGTMAQVHVAT
                     LADGDEVVVKIQRPTARQDILQDLGLLQMFAEKTRDKKALRAVVDLPAIVDQLSESLQ
                     RELDFSIEAKNAERLREVLTLYSRLDVPRVYTEFSTERLLVLEAIRGVPVHEAPLGPE
                     RIEAARQLLESYYRQVLGDGFFHADPHPGNLLWADGKVWFLDVGMVGELGPEVRELLV
                     LLIMAFWQQDVAFLSDMVLMLTGEDQRQDLDLHAFQEELGHMVDRYRGLSLRDIQLGP
                     ILQEITEISVRHQVRLPASLALTGKALAQVQLIVSDLDPELDPFSVAGSFLVRNVGSR
                     VLSRLDPQKLFYEGQKLKVRASRMIETLER"
BASE COUNT         2200 a         5506 c         5128 g         1931 t
ORIGIN      
        1 agccggtgga gggccgcggc cgcgaagggg tcccgctgct ccatctcgtg gagtcggtcc
       61 cgggtgaggc ggtgcaggac gctgggccgc tcggccacca cggtggcgga gcggacgccc
      121 cccgcgtaga gcgctacctc ccccaccacg gctccgggac ctagggtctg gaggcgcacc
      181 gggcctccgc gctcccgggt cacctgggtg gtcacctgtc cggactcgag gaagaggacg
      241 tcctccgtcc gatccccctg gcggatcagg atctccccgg cgggggcctc gatacgttcc
      301 atgtacccca ggaggacgtc ctcggggatt cccacgcccg ccagcgcctc ccgccacggc
      361 gtgcccggtg gctcctccgg agccccctcc tcgtccagaa gcccatcctc acaccactgg
      421 aggccgcggt cgagatcggg gaagaaccgc acggacgggt ctccctcggt gagcccgccc
      481 tgctcgagcg ctcgccgaac accatccccc atgctggtga ggaccatcgc gaagccggac
      541 gaggccgcca ggcgctgggt cttggagaag ctcagcacca ccgacgagtc caggccgatg
      601 acccgtcgga agtccagcac caggaaccgg agcggtggaa gcttcccgtc ggtcgcccga
      661 gcccggatcc ggtcgaggag cgcgttggcc gttccgaaga acaggaagcc ctggagctcg
      721 aggacgtgga tccggtcccc gtccctccga aggacctcac gatgggcgag cggccgatcc
      781 atcttgctgt ggtaggtggc gccgctgagc gcgtgcttca ctacgtcgct ccttccgtag
      841 gcgacggcga acagaaccac cgcgagcagc acgccgagcg ccacgccggg gaggaagccg
      901 accgcggcga ccaccaccag gatcagcacg acgatcaggt actcggatcg agggagcagc
      961 gcccacgcgt ccaccaccca ctcgaccagg aaggccaacc ccaggaacac gatgatcccg
     1021 ccgaggactg gtcgggggaa catgccgagg atgccgcccc ccgccaccag agccaccacg
     1081 cacacaccgc cggctacgac cccggccgcg cggctccggg cgcccatctt gtacgccaac
     1141 gccgtcaggc tcagcgcgtg gaacccgggg aggccgcccc cgagccccgc agccaggttc
     1201 gcaatccccg ccgctcggag ctctcggttc agatcgaggt cgcggcccac caccagctcg
     1261 atcccgctgg cgttcaggag cagggccagc agggagacga tgaggatcgt ggcgaggttc
     1321 ccggcctctc ccacgaccgc tgaccagtcc gcctgcgcga cggagcgggc gacccacggc
     1381 tcccacaggc ccccctccgg gaacgggccg agcagccagc cggcctcctc cacggccccg
     1441 atgcccgcgc cggcgaccag gaccaccacg tagaatgccc cgatcccgcc gagcacgctg
     1501 agggggatca ccatcgggtg ctgcgaccgc cgcgccagga ccagcaggac cacggcgaag
     1561 acgacgccgg ggatccaccg cgcgagcacg tccgattccg cgagcacccc cagacgcccg
     1621 aacgcgaggg gcacgccgct cacgacctcc atcccccctc ggaacaggag ccagcccgtg
     1681 ccggccagga agccaccgac gacgggatag gggacgaacc ggatcacgtc ccccaggcgg
     1741 aaggtcccaa gcaggaggaa gaacccgcct gtcaggagac tcgtcaacgt gatggccgcg
     1801 acgaccgtga ggaagacgcg agggtcttcc gggggaaggt cggccacgat ggacgcagcc
     1861 acgatggaca gcaccgcggc ggagatgtcc tggacgctgc cgaacgtgcc ccgatggcta
     1921 ctcacgagag ccacggtgat caggatcgcc gcggcagcca cgagtccgag gccgatgcca
     1981 gccgggaggt gctcggccag cggcccgctg aagatgagag cggcgaagga cgtggccacg
     2041 accacctcga ggacccccac caccacgccg gccgtcaggg tcgggaagag actcgcccca
     2101 cgccccgatc ggagaggatg tccgtcatgt gccgtcgccg tcgcggagcc ctccgcggac
     2161 gtcgccatgg tccggtcccc cttcctcccg agcggcctcc gcgatggtgg cacacgcccc
     2221 gcgccgaagc gagaggaggc ggcggccgca cctctacgct gaccgcaggt cgagccgcca
     2281 gtggttcacg cgtagggtga ctcgagccgt gtagtcgcgc gctcccacga gcgtgaaccc
     2341 cagcgtccga cacatcgcgt tcgaggcggc gttcgacacg ggagggaacg cgtgcagcac
     2401 gtcccaccgc cccttctccc gggcacggtc gagcgccatc cgcacccccg cgctccccac
     2461 gcccttcccc tggtggtcgg ggagcaccat ccacccgatc tcggtgatca cctcgccgtc
     2521 cagctcgtgc ggccagatcg ccacggttcc cgcggcggtc tcctcctcga ggacgatcac
     2581 gaggacccac acctcgtcgg cttcggtggc ggccgcgtcc cgctcgagct tctcggccag
     2641 cccttccttc gggtgtggac cgcccaggtg ctcccacatc cgagggtcac ggtagatcgc
     2701 ctcgtagagc cagagatcct cggtcgtgag gttcctcagc cgcacggctc agcaggacgc
     2761 ggggagggac tggccgtcct cggggaccgc gacgaaggcg atctcgcccg gtctcaccat
     2821 ccccaggcac tgacgggcga tgcgctccag gtactcgggc tcctgcagcc gctccacgcg
     2881 gtgctccagc gtggcgcgct cctcctcgag gcgctgcacc tttcgctcca gacctgcgat
     2941 ctcgctccgc tggatcacgt actggcgaag cgggaggacg agcgccagca gcaggacgaa
     3001 cgagatggcg gcgaggccgg ccgcgcgacg gctgagcctg ggtcggggca gggcgtgacc
     3061 cctcgcgctc atcgcccgaa cctccggtag accgcggcgc ccgcgtatcg ggccgtgtcg
     3121 cccagctcct cctcgatcct gaggagacgg ttgtacttcg ccgtccgctc gccgcgggag
     3181 ggggcgccgg ccttgatctg gcccgcgttc gtcgccacgg ccacgtcggc gatcgtggag
     3241 tcctcggtct cgcccgaccg gtgcgagatc acgacgccga aactcgaggc cacggcggcg
     3301 tccacgacgt cgagggtctc ggtgaggctc ccgatctggt tcggcttgac gaggatcgcg
     3361 ttgcaggagg cttcggcgat cgcgcgggag aggcgctcgg gattcgtcac cagcaggtcg
     3421 tcgcccacga gctggatccg gttgccgagc aggctcgtga cggccgccca gccctcccag
     3481 tcgttctcgt cgagagggtc ctcgatcgac acgataggga agtcggtcac gaggcggtcg
     3541 tagtactccg ccatctgctc ggccgagcgg gcctccccct cgaggtggta cgagccgtct
     3601 cggaagatct cgctggccgc cgcgtcgagc gcgatcgcca cgtcggatcc gggctccagg
     3661 ccggccgcct cgatcgagcc caccagggcc tcgcaggccg cggccgagcc ggggaggtcg
     3721 ggcgcgaacc cgccctcgtc gcccacggcg gtggacaggc cctttccgtg gagctgctcc
     3781 ttcagggtgt ggaagatctc ggagccccag cggatcgcct ccgagaacga cgccgccccg
     3841 cggggcgcga gcatgaactc ctggagctcg atcgcgttgt ccgcgtgcgc gccgccgttg
     3901 atgacgttca gcaggggcac ggggaggagg tgggcgttcg ggcctccgag gtagcggtac
     3961 agcggcagcc cggacgagat cgcggccgcc ttggcgttcg ccagcgacac ggcgaggatc
     4021 gcgttggccc caagaccgga cttgtcgagc gtcccgtcga ggtcgatcat catgcggtcg
     4081 acctcgcgct gatcgagggc ctcgacgccc tggagcgagg gctcgatgcg ttcgtggatg
     4141 ttctcgatag ccttcaggac gcccttcccc ctgtagcggt cgccgccgtc gcgcagctcc
     4201 aggacctcgt gctcgccggt cgaggcgccg gacggcaccg cggccagggc gagcgagccg
     4261 tcggccagcc cgacctcggc ctccacggtg gggttgccgc gggaatcgag gatctctcgc
     4321 gcacggatgc gttcgatggg tggcatgggc atccgattct aggacgggga gggcccccga
     4381 acacgcacct tcgtcagacg gcgcgcgcga gcacgtggtg gtaggcgaac ccctcgtcct
     4441 ccaggggctg ctcctcctcg tcttcgatcg cgaagccggc ttccctgagc cacgttcgca
     4501 cccgatcgag ggacgggtag tggtggtagc cgtactcctc ccagatcacc tccccgtccg
     4561 cgagcgggag gccgcgccgc cgtccctcct cggttcccgc ccggacctct tccggaggcg
     4621 cgatctcgac cgtcaggtag agggggcccc ggccggcgat cgcccgacgg aatcgacgca
     4681 ggacgtccgg ccaatcctcg ggcgggatca tctccatggc atcgacgcac atcacgccgt
     4741 cgaacgcctc ccggtaggcg aggtcctgga ggtcgtgccg ctccgtctcg gccccggggt
     4801 gcttcgcctt cgcctcgacg agacaggccc cggcatgatc cacgcccagg acggagcgac
     4861 cgctctccag gaccatgccg aggtacttcc cggtgccgca cgcggcatcg agcacccgcc
     4921 cggcgggcgg gaccatggac aggaaccgct cgacgaaggc tcggtgcgtc tcgtcgatct
     4981 cgccccagcg ctcgtcgtac gtcggggcga gcacgtcctc ctcttcctcg ttcacgcgcc
     5041 ggagctcgac gatccaggtg gcccgatcca cgcgggccat cgtgccacta ctgctggagc
     5101 gccgtctccc gctctacctc gggctcgacg ctgcctcggg ccgcgagcgt ccccaccccg
     5161 atcagcacga gcgccacgcc gagccactgg accagggaca ggatctcgga gaacagcagg
     5221 taggcccaga gcacggtcag catcggctga agcaggagca tcaccgaggt ctcaagcgag
     5281 ggcaggcgag ggagcgcgac ggtgatcagc agccacccga agacctgaca gatcagcgcc
     5341 agcgccagca gccagccgtg cgccggccag ctgggcgcca ccgagaacga gggatcgaag
     5401 accggcccga tgatcgccat cccgatgagg gcgccgaccg tggcctccat cagcggaccc
     5461 gcgggagggg ccaggccacg gttcgacgcg cgcagcagca ccaggaaggc cgtgtacatc
     5521 agtcccgtca gcacgcccca gaacacgccg gccagcgggt cgctcccgaa cgcgtcctcc
     5581 cgccccaggc ccgagatcag caggacgccg gcgaacacgg tcggcacgag gacgaacgcc
     5641 agccgggtcg gccgctcgcg caggatcatc cacgcgtaga tcccgacgaa cacaacctgg
     5701 gtgttcccga tcaccgtggc caggcccgcg ccgatgtcct cgatggcgcg gtgccagaaa
     5761 gcgaagtcga gcgcgaggaa gagcccggcg gcgaaagcca tccaccgctc gcgggtcgtc
     5821 cgccggtcgc ggtcccgaaa gacgagccac acgaggacga gcgcagggac cgcgtaggcg
     5881 gcccggaaga acgccgccgt gtcgggggag acgtcggcga gcctcacgaa gatggcagag
     5941 aacgagatgc tgacgatgcc gaccagggag agcaggtggg tcacgcgcgg gcctcgcgga
     6001 accgccgcag gaggtcgtcc tccgggaggt cctcgagccg taccccttcc tcccgggccc
     6061 gctcgtccat cgtctcgaag cgctcgacga acttgcggat cgtggagcgg agggcgctct
     6121 cggcgtccac gcccagcttc cgggccaggg cggtgacggc gaacaggacg tcgcccacct
     6181 ccccttcgag ggcgtccttg gcctcgccct cgacgtcccg gccggcatgg gcggcggccg
     6241 cctcctcgag ctcggtcagc tcctcgcgga ccttctccac ggcgccggcc agcgcggccc
     6301 agtcgaagcc ccggcccgac gcccggcgct ggaccttctg cgccgcgatc agggcgggga
     6361 gggccttggg gaccccctcg tccacggcat gctcgccctt ctcctcggcc ttgatccgct
     6421 cccagttcgc gtagacggcc tcgggggtgt cggcttccac ctcggcgaac acgtggggat
     6481 ggcgccggat cagcttctcc acggtgacct cggccacgtc gtccacgtcg aaggtcccgt
     6541 cgtcggccgc catctgcgcg tggaagatcg cctggaggag gacgtcgccc agctcctcct
     6601 tgagatcgtc gggcgtgccc tcctcgatcg cctcgatcag ctcgtaggcc tcgtcgagga
     6661 ggtgacggcg gagcgacatg tgggtctggg cccggtccca ggggcagccg tcgggggcgc
     6721 ggaggcgagc gaccactcga acgagatcga ggagtcgctc gcctccgcgc cccgacggca
     6781 cggggcccgt ggggacgtgg tcgagcggat cgggggtgcc gtggacctcg gccacggcgc
     6841 ctatcctact gccgtgccgt cgagcacctc gcggtcgccg acgcgctccc ccaggatcac
     6901 ctcgatcccc ttgagggccg cgatctcgat gcaggtcgcg gcctccggat cccgtccttc
     6961 gtacacggtc agggtgacgg cgtcggaccg ctcctcgacg tccacgcgcg ccactccgta
     7021 gcactcctcc accccgccgt agaagaccgc cagcaggcgc ctcgggccca ggcgctcgat
     7081 ccgctcgagg gtcaccggcc gcacgtccac gaggccctcc cgcagggtga cgatctgggg
     7141 ctcgccctcg gtgagcgggc cggtcggcga tggcgccgtc gtggaggtcg gctcgtcggg
     7201 cccgggtgtg ggcaccgttc cggcctcgtc gccgccgcag cccgagacca gcgccgccgc
     7261 gaccgccacg gccaggaggg tgcgcgcggc tcccgcccac gcgctcaggg cgtcgtctgc
     7321 gggcccggca gcggcgtggt cgccctccgc tcgacgacct cccccgtctc ggcgtcgaac
     7381 gtcccgtacc gggggttcac gcggacggtc agggagcgga gccgctccag catccagtcc
     7441 cggaagacct cgggccccag ctcctggagc agctgctccc tcgcctgctc gaaggggatc
     7501 gcctcgcggc tgtgcaccag gatcacgtgg aacccgaact gggtctgcac cgggcctccg
     7561 atccggccaa cagggagctc cagcagggca tcggcgaact ccgggacgta agcgccggcg
     7621 gcccgggtgc ccaggtctcc gccgttcgcc cccgatccct ggtcctcgga gagctcccgc
     7681 gccagccggg cgaagttcga gcgcgtggcc tccgccacga tgcggctcgc ctcctcctgg
     7741 ctgctgacga ggatgtgcga ggcgcgcacc tcggtgaact ctcgggcccg ctcctcgtag
     7801 gtggcctgca gccgctcgtc ggaggcctct tcctcgacca ccgcctggcc cacgtcgctg
     7861 cggatcagct gggcccgcac gaacgcgcgg acgtcctcga cggtggcgtc cgagcgctcg
     7921 atctggtcct ggatcgcctg atcctgcatc agctcctcaa ggcgccggcg gtccgcctgg
     7981 atgccttcct cgcgcgccct ctcctcgacc acctcgtgct ggatcaggaa ggtgagcagc
     8041 tgtcgctgga gcgtggcccg ctcgacgtcg gggttggcca cggccgcctc cgcgaactga
     8101 gggtcggcca tgatgaagtc gaggagcctg aggaactcgt cgtcctcgat cctgtggggg
     8161 ccgacgaccg cggcggccgg ctcgaaggcg gcgcagcccg agaggacgag cgagacggcg
     8221 gcgaggaggc tcaggaggcg caggcgcacg cgccggattc tagccggatg ccgccggctc
     8281 cgtccgggcg cccgaggcct cgacgagccg gtcccggagc caacgggcta ggtcggcgcc
     8341 ggccagggcg ggcgggtcga ggttgaaggt gctcgtcgcc gcgtgccagg cggtgcgctc
     8401 gggcaggtcc tccacgtccg ggacgaaggg ccggacccgg acctggcccc ggaaggtcga
     8461 gacctcctcc accccgtggc gctcgcaggc gatgcggagc cgcgtgagct cgatcagggc
     8521 gtcgccctcg ggcggcaggg gcccgaaccg gtcctcggtg gcgctccgga tccggtcgag
     8581 gtcggcctcg tcccgggccc cggcgatctc gcggtagagg tcgagccgca gggactcctg
     8641 gcccaggtag tccgggggga ggtaggcctt gatcgggagg tcgatccgga ccggggcctc
     8701 ctcggggagg ggctcaccct ggagctctgc caccgcctcc tggagcagcc gggcgtaggt
     8761 gtcgaacccc accgcggcga tgtggcccga ctgctcggca cccagcaggt tgccggcccc
     8821 gcggatctcg aggtcccgca tcgcgatctg gaagcccgag cccagcgcgg tgtgtcgccc
     8881 gatcgccgac aggcgggcgt gggcctcctc ggtcagctgg cgctggggcg ggaagaagag
     8941 gtaggcgaag gcgcgctcgg ccgagcgccc cacgcggccc cggagctggt acagctgcgc
     9001 gaggccgagc gcgtcggccc gctcgatcac gagggtgttg gcggtgggga cgtcgagccc
     9061 cgactcgatg atcgtcgtcg tcaggaggac gtcgacctcc ccgttccaga aggcgagcat
     9121 cgtcttctcc aggaccttct cgtccatctg gccgtgggcc acgccgatcc gggcctcggg
     9181 gacgcgttcc gccagccagc cggcccggcg gtcgatcgtc ctcgtgtcgt tgtggaccca
     9241 gaagacctgg ccgccccgcc gcagctcgcg gcgggccgcg cccagggcca cgtcgtcctc
     9301 ccaggagccg acgtaggtca gcaccggctg gcggtcctcg ggcggcgtgt ccaccacgct
     9361 catgtcgcgg atgcccgtca gcgccatctc cagggttcgc gggatcgggg tggccgtcat
     9421 cgtcaggacg tccacgtcga ggcggagctg cttcagccgc tccttgtggg ccacgccgaa
     9481 ccgctgctcc tcgtccacga tcaggagccc gagatccttg aagcgcacgt cggccccgag
     9541 caggcggtgg gtcccgatca cgacctcgat ctcacccgag gccagcccgt ccagcacttc
     9601 gcgctgctcc ttcgccgaca ggaagcgcga gagcatcgcc acccgcacgg ggaagggcgc
     9661 gtaccgctcg gcgaaggtca cgaagtgctg ctcggccagc agggtggtcg ggacgagcac
     9721 cgccacctgc ttccccgagg tcgccgcctt gaacgcggcg cgcagggcga cctcggtctt
     9781 cccgtagccc acgtcgccgc acagcaggcg gtccatcggc ctggcgacct ccatgtcgcg
     9841 cttcacttcg tcgatcgcgg tgagctggtc gggggtctcc tcgtacggga aggccgtctc
     9901 gagctcgtgc tgccagacgt cgtctggggg gaaggcgtga cccttcgcgg ccatccgagc
     9961 cgagtacagg cggacgagct cgccggccat gtcgcggagg gcccgcttca ctcgggcctt
    10021 ggtgcgcacc cagtcgttcg tcccgaggcg gtgcacgcgc gggctctcgc ccccgacgta
    10081 cttggagacg acgtcgacct ggtcggctgg gacgaagagc cggtccccct tcgcgtactc
    10141 caggaggagg taatcgcgct cggcgtcgcc gattctgcgg cgcgtcatcc ctgcgtaccg
    10201 ggccacgccg tgaacgcggt ggacgacgaa gtcgccgggt gacagctcca gggcgaacgc
    10261 cccggccgac ctccccgcca gtcgcggcgc ctcgtgcgtg cggcggcgtc gcccgaagag
    10321 gtcgtcctcc cccaccaccg ccacccgccc ctccgggaag acgaagccgg tcacggggcc
    10381 gtcggagatc gcgggatcgg gcgagacccc ggcgcccgac agcacctcca cggcgcggtc
    10441 gaggctgccg tggccctggg cggtggcggc caccgtgtag ccctcgccgg cgagcgtcgc
    10501 ggctcggctc gccacccggc ccgggtcgcc cagggacggc ccccacccct cgatcccgag
    10561 atccacgccc tcggtgaagt cggagaggtc gacgtgggcc atgccctcga gggcctcgcc
    10621 gggagcggcc atcgtctcaa ccccggccca cccgagcgcg tcggccaggg cctcgcactc
    10681 ccggcgggcg gactccgcgc gctcctcggc cgtgcgcggc gccgtcacga cggcccagac
    10741 accgccgggt agccggtccc gcaccagcgg gaggtcctcg aagatcagcg ggccggcctg
    10801 ttccatcccc tcgaagagga ggccctgcga gacgcggtcc accgcgtcgc ggaagcggtc
    10861 cttgtggcgt ggaacggcct cgagggctcg ctccatgacc tggggcgtgg ggagcagctc
    10921 gcggacgggg tcggcctcga gccgcgagag cctcccggtc gaggtctggg tggatgccga
    10981 gaactcgcgg agggactcca cctcgtcccc gaacagctcg atgcggaccg ggcggggcgc
    11041 ggccccgggg aagacgtcca cgatcccgcc gcgcacggcg aactcgcccc ggtgggtgac
    11101 gagatccgag cgcgtgtagc ccagggcgac aagacgatcc gccagctcgt cggggccgat
    11161 ctcctggcct gcctccacga cgagcgggtc caccgcaccc acctggggga gcagcctctg
    11221 gacgacggcc acggccggcg ccaccagcac gaacgcgcca cgggcctccc gggctcggcg
    11281 gatcgccgcg tggcggcggg ccgaggtctc cggcgagggg ctgatcccct ccactgggag
    11341 cgcctcccaa gcggggaggt gggccgcgcg atcggcgccc aggtacgcgg ctacgtcgtg
    11401 ggccaggggc gccgattcgc gcggcccagc cgtcaccgcg agcgccggcc cctccagcgc
    11461 ctccgccagc gccgcgatcg cgtaggcgtg accggccggg tccacccgca ccgggcccgg
    11521 ccgcgcgagg accgcctgca gggcctcgct ctcgatcagg ggttccagca gcttccgcac
    11581 agcacgatgg ggcctccgcc ggcctcggcg gggccctcga tctccttccc ggttccgtcc
    11641 cggccggatg ccgggcgcgc tcccatccta gtgctcaacg gcccttctcg aagcgccgat
    11701 gggctgatca ggccgggatc tctggcaccg ggaactccgg ctcggcgcga cgcctccggc
    11761 gtcgtggacc gaggcgcctg cggcgccgca ccgccagcat cacgagcccg agcagcgcca
    11821 cccccgccgc ggtgaagtac acggaccgca ggcccagcgc ctcggcgacg tacccgagcg
    11881 tcagcggccc caccccgaac gcaaggtcca cgaacgccga gaaggtaccc accgccgcgc
    11941 cgcgctccgc gggcttcgta ttggcgatcg tcatcgacat cagcgcgggg aaggcgaggc
    12001 ccacgcccac gcccaggatc gccgtaccga tcaacaggcc cggcgtcgac tcgaacgtcc
    12061 cgatcacgag gagcgccagc gccgacgccg tcagcgcagc gcgagccgct cgccccggac
    12121 ccagccggtc ggggagcttc gccccgaacc cccggatcag caggaccacg cccgagaaca
    12181 gcaggaagac gaagcgcgag tcctccagcc ccagtcgctc cacgtacagc ggcgtgaagg
    12241 cgaagaaccc ggccatcccc cagatgctgg ccaggatcac gaggcccggg acgaccgccg
    12301 ccgggtggac gatccgcatc ctgccctcgc tgcgctcggg cggcctcgtg tcggggacgg
    12361 gcacggcgag gacgaacgcc agcacggacg cccccacgat cagccaccag gcgaggtcga
    12421 agcccgacga cgagacggcc acctccccca gcaccgggcc cgccccgacc cccgcgtaca
    12481 gcgagagcga gaagaacgag atggcctcgc cccgacgagc ctcgggggcg aggtcggtca
    12541 ccgcggtggc cgcgcccacg aagaagaacg cggcaccggc gccggtcatg agccgcatga
    12601 ccaccatcag cgggatcgac tcggccacga cgtagcccgc caccgacgcc gcgaagacca
    12661 ccatcccgac cagcatcacg aagcggcgac cgcgacgatc ggccagccgc ccggcccacg
    12721 gacggaacag cagcgcggtg accgcgaacg cgccgaccac gatccccacg gccgactccc
    12781 cgcctccgag cggcccctcc acgtaccggg gcacggtcgg gatcagggcc ccttcggcca
    12841 cgaagtaggc cagcgtcgac agggcgatga gcaggaaggc cggcgtcacc agcctcggcc
    12901 tcgtcgccgc ccgcgtcacc cttctccccc cacggcgggg caggctagca cctcgcccgc
    12961 ggtatcatcc gctcgccgtg cagcgcatcc tcgtgggcac cgaccgatcc tcctccgccg
    13021 accgggccgt ccgctgggcc gccgacctgg cctcccggtt cgagtcggag ctcttcgtgc
    13081 tgcaggtgat cctccccgaa caggagaccg atatcgagga gggagcccgg gcccagcacg
    13141 cgttcgagga gctgcgcctc ctggccgagg aggtcgccgg cgcccggggg aaggcccgcg
    13201 tcgtggtgga cgacgatccg gccgcagcga tctgcggcgc ggccgccgag gaggaggtgg
    13261 acgcggtggt cgtgggcaac ttcggcatgg ccggccgcaa gcggttcctc ctgggcaaca
    13321 tcgccaaccg cgtctcccac agcgccacct gcacggtgat catcgccaac accgtctcgc
    13381 ccgaggaccg ggtggtccac ccgacgcgcg aggagcggga ggctgccgag accgagcccc
    13441 acctcctgcc ccgcgccgcg cggatcggca gggtcatggc caaggccggg ctccgcgacc
    13501 tcttccggcc cggcgcctcg gaccaggacc tccgcgaccg cgccgggaag ctgcggaccg
    13561 ccttcgagga gctgggcccc accttcggga agctaggcca ggtgctcgcc accagacccg
    13621 acctcctccc ccagcccttc atccacgagc tctcgagcct gcacagcagc gtcccgcccg
    13681 tctccgagga ggagatcgtg gcggtgatgg agcaggagct cggggtcccg tgggaagacg
    13741 tcttcgagag catcgagccc gagcccctgg ccgcggggac gatggcccag gtccacgtcg
    13801 cgacgctcgc cgacggcgac gaggtggtcg tcaagatcca gcggccgacc gcgcggcagg
    13861 acatcctcca ggacctcggg ctgctgcaga tgttcgcgga gaagacgcgg gacaagaagg
    13921 cgctccgggc cgtcgtggat ctcccggcga tcgtggatca gctctcggag tcgctccagc
    13981 gcgagctcga cttctcgatc gaggcgaaga acgccgagcg gctccgcgag gtgctgacgc
    14041 tctactcgag gctcgacgtc ccccgcgtgt acacggagtt ctccaccgag cggctgctgg
    14101 tcctggaggc gatccgcggg gtccccgtcc acgaggcgcc gctcgggccc gagcgcatcg
    14161 aggccgcccg tcagctcctc gagtcctact accggcaggt gctgggggac ggcttcttcc
    14221 acgccgaccc gcaccccggg aacctgctgt gggccgacgg caaggtctgg ttcctggacg
    14281 tgggtatggt cggggagctc ggccccgagg tccgcgagct gctggtcctg ctgatcatgg
    14341 cgttctggca gcaggacgtg gcgttcctct cggacatggt gctgatgctg acgggcgagg
    14401 accagcgcca agacctcgac ctccacgcgt tccaggagga gcttgggcac atggtggacc
    14461 gctaccgcgg gctgtccctg cgcgacatcc agctcggccc gatcctccag gagatcaccg
    14521 agatctccgt ccgccaccaa gtgcggctgc cggcctcgct cgcgctcacc gggaaggccc
    14581 tggcccaggt ccagctgatc gtctcggacc tcgaccccga gctcgacccg ttctcggtgg
    14641 ccggctcgtt cctggtcagg aacgtggggt cgagagtgct gagccggctc gatccccaga
    14701 agctcttcta cgagggacag aagctgaagg tgcgggcctc gcggatgatc gagacgctgg
    14761 agcgg
//
LOCUS       DASUTZ010000200         6549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1881516, whole genome shotgun sequence.
ACCESSION   DASUTZ010000200 DASUTZ010000000
VERSION     DASUTZ010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6549
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1881516"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VF097_08785"
     CDS             complement(<1..395)
                     /locus_tag="VF097_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262926.1"
                     /translation="MADHREAEDRERRARLIDERLSPVMAVLGILFLLVVVGQALAMP
                     GSGLRHTLATLIWVLWGLFVTEFFVRLWVAPSAGRYLRRNWWQVLLLAIPVLSFVRLL
                     ILARLARMGRLVSSALRGTRSARSRLGSRL"
     gene            459..827
                     /locus_tag="VF097_08790"
     CDS             459..827
                     /locus_tag="VF097_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006607936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HEX6262927.1"
                     /translation="MERVDLLVFDGACGFCTRAARWVQRRLPPGARAVPWQRAPLDRL
                     GLSPAEAARMVWWIDADGTRRAGHLAVAAALRAIGGGWGALGVAIRLPPVRWVAAGVY
                     RLVARYRGRLPGTTPAVRDR"
     gene            838..1200
                     /gene="paaI"
                     /locus_tag="VF097_08795"
     CDS             838..1200
                     /gene="paaI"
                     /locus_tag="VF097_08795"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HEX6262928.1"
                     /translation="MIDPLSRLLGLELLEEGPGRATVRGRVSEDHLNPHGMAHGGFIF
                     SLADAALAVASNSHGPEAMAIAATIHFTRPAKAGDLLTAQAREVSLGARTATYEIEVV
                     SGEGTPVALFTGTVYRRG"
     gene            complement(1208..1594)
                     /locus_tag="VF097_08800"
     CDS             complement(1208..1594)
                     /locus_tag="VF097_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017199277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX6262929.1"
                     /translation="MGEQENRRVIERLIQCINDGKIEIMDELFHEDAVMDWPQSGEMV
                     VGGDNRRAIYGAFPQLPTITPRRMVSGGDLVVAEASLDYGGGTVYATVFIFELRDGRI
                     ERETAYWSEAFEPPDWRSQWVQVAGA"
     gene            complement(1655..4504)
                     /locus_tag="VF097_08805"
     CDS             complement(1655..4504)
                     /locus_tag="VF097_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX6262930.1"
                     /translation="MIRLRAALRVRPGEGGLVLRFLAVMLVGLFGATLGGAAVESLFF
                     SRFGPHFLPYLYMALGVATFFVMAAMSAVLARPHPGRVLATLPLGLAVLLLAARTTLL
                     LDQRWLYPVLWVVMMIAWTTQVMVSWGLAGDVHDTRQAKRLFPLYGVGLILGGIAGGL
                     VTGPLAALLGTENLLLIWAAALITVFALGRAVVGPGVRRRRRGRRASVLAEAGEALRQ
                     VRAAPLLRWLSLALMLFALLYFTLSLQFAEAATARFPRTEELAGFLGGFMAVSNAAAL
                     LLSLLVANRLFARFGVPAMVLALAAIYAVGFGVLVAASGFAILVGFRFVQMVWVNGVW
                     GTGWQALYNVIPSETRARIRSFMDAGPLQAGVVLSGAMLVLADRVLSGRALAALGVAA
                     AGLTAWAMWRARRAYGEALEVALRAGNPDVFGSEDEPFTGRAWDAEAVQAALGGASTR
                     DPVVRRISMEVLSRLDAPPALPALLDALSDPDAEVRAAAARGVGRAGGPSHAAAIEAL
                     LDDPDAGVRGSAVQAMRALGGGAASTSLRPLLTDPDPEIAARAAATLVASGDEQALDL
                     LVEMAVAVEPDRRRAAVTALAETGADPDIVLERMSDPDVSVRRAAVAAAGSLEAGGAA
                     RAVLQALQDPHPSVREAAVEALRDIRHADPDLLRAYVERETGEALRYDGLWRALGNDG
                     DPRMGLLASAVREESLTHARNALRAAALTGDPSAVELAAANLESRDAQQRANALEMLE
                     AVGPRALVRPIVALWEGAPEPRGDRARALLRLLDDADPWLRSCAAFAAATTEGGKQME
                     TLATLSAMDRVLFLRNVPLFADLTPRDLGRIAGVATEHVFQDGELIAEQGEPGDEMHV
                     VVSGEIRAIVEDDAGGRQEVGRRGPGDYVGEMAIISDQPRMASLVCVGDVRTLALDRK
                     RFERILTDRPEASLAVMRVLCDRLREAHGQLPSSA"
     gene            4519..6477
                     /locus_tag="VF097_08810"
     CDS             4519..6477
                     /locus_tag="VF097_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6262931.1"
                     /translation="MPRPAEDAIAAGREALERHAWQEAYENLSEADRRGSLAAEGLAL
                     LAESAWWAARPDDVIEALERAYAAYLKEDDRREAAMMAFRVAEQHGMRMAGPQAQGWA
                     SRAAELVADDPDAPVHGWLAWIQGLMTLAMGGDEEESLGHYDRALEIARRLGDRDLEA
                     MSLHDMGHILCSRGQVTEGLALMDQVMVAAVGGELDPTATGYVYCGMIGICSRLGDYG
                     RAAEWTEATMRWCERHSITGFPGVCRVHRAELLRLRGALEQAEEEARLACDELPRFNL
                     YSGLGHAWYEIAEVRRRMGDVAAAEAAFAAAHEHGKSPQPGLSLLRLAQGRVEAAAAG
                     IAQALAEAGDDRLARMRHLPAQAMIALEAGDLETAAAAAEELAEIVEGHGAPAMRATL
                     SSVRGALLLARGDAEAALPHLREARDGWRGLDAPYEIAEVRLLLARACRATGDEDGAR
                     MEARAARSAFETLGARPAAEAAAELVGTPTAAERPERVRRAFMFTDIERSTDLVGVIG
                     DEAWESLLGWHDRTLRSLFASHGGEVAHHTGDGFFVSFEDPASALACAVAVQRALAEH
                     RRTHGFAPRVRIGVHTAEATRRGQDYGGVEVHKAARIAAEARGGEILASEETARGGGT
                     TVSGDPRTVSLKGISEPVRVVPVRWRDG"
BASE COUNT          952 a         2431 c         2365 g          801 t
ORIGIN      
        1 agccggctcc cgagccgcga ccgggccgag cgcgtgccgc ggagagccga ggagacgagc
       61 cggcccatcc tggccagacg agcgaggatc agcagccgga cgaagctcag cacggggatg
      121 gcgagcagca ggacctgcca ccagttccgc ctcaggtacc gaccggccga aggcgcgacc
      181 cacagccgca cgaagaactc cgtgacgaac aagccccaca ggacccagat gagcgtggcc
      241 agggtgtgtc ggagcccgct tccgggcatg gccagggcct gccccacaac gacgagcagg
      301 aagaggatcc cgagcacggc catcaccggg ctcagccgct cgtcgatcag ccgagcccgg
      361 cgctcgcggt cctccgcctc tcgatggtct gccatggatg atggatgagt ctccgcaaca
      421 cgcgtggtct aacccttgcg gggtaccgtg gagccgtggt ggaacgggtg gacctcctcg
      481 tgttcgacgg ggcctgcggc ttctgcaccc gcgcggcgcg ctgggtccag cggcgtctgc
      541 ccccgggtgc ccgcgccgtc ccctggcagc gggcgcccct cgatcggctc gggctctccc
      601 cggcggaggc ggcccgcatg gtgtggtgga tcgacgccga cggcacccgt cgagccgggc
      661 acctcgcggt agccgcggcg ctccgcgcga tcggcggcgg ttggggggcg ctcggcgtcg
      721 cgatccgcct cccgcccgtc cgttgggtcg ccgccggcgt ctaccggctc gttgctaggt
      781 accgaggccg cctgccaggg acgacgccgg ccgtccggga ccggtagcct gcggcccgtg
      841 atcgatccgc tgtcccgcct gctcggcctg gagctgctcg aagagggccc gggccgcgcg
      901 accgttcgag ggcgcgtctc cgaggaccac ctcaacccgc acggcatggc ccacgggggc
      961 ttcatcttca gcctggccga cgccgccctc gccgtcgcgt cgaacagcca cggacccgag
     1021 gcgatggcga tcgcggcgac gatccacttc acgcggccgg cgaaggccgg ggacctgctg
     1081 acggcccagg cccgcgaggt ctcgctgggc gcccgaacgg ccacctacga gatcgaggtc
     1141 gtctccggcg aggggacccc cgtggccctg ttcactggga ccgtctatcg gcggggctga
     1201 gctccgatca ggcgccagcc acctggaccc actgcgagcg ccaatccggc ggctcgaagg
     1261 cctcgctcca ataggccgtc tcccgctcga tcctgccgtc ccggagctcg aagatgaaga
     1321 cggtcgcgta gacggtcccg ccgccgtagt cgagggatgc ctcggccacc accaggtcgc
     1381 ccccgctgac catgcggcgg ggagtgatcg tgggcagctg ggggaaggcg ccgtagatcg
     1441 cgcggcggtt gtccccgccg accaccatct caccggactg cggccagtcc atcacagcgt
     1501 cctcgtggaa cagctcgtcc atgatctcga tcttcccatc gttgatgcac tggatgagac
     1561 gctcgatgac ccggcggttc tcctgctcac ccatccccaa cctcctgtcc gtgacggacg
     1621 tatctttgca cggagcaggc accggtcgac ccagtcaggc cgacgagggc agctggccgt
     1681 gtgcctcgcg caggcggtcg cagagcacgc gcatcacggc gaggctggcc tccggccggt
     1741 cggtgaggat ccgctcgaag cgcttgcggt cgagggccag ggtccgcacg tcccccacgc
     1801 acacgagcga tgccatcctc ggctgatcgc tgatgatggc catctcacca acgtagtcgc
     1861 cgggaccccg gcggcccacc tcctgccggc cacccgcgtc gtcctccacg atcgcccgga
     1921 tctcgccgga gacgaccacg tgcatctcgt ccccgggctc gccctgctcg gcgatcaact
     1981 cgccgtcctg gaagacgtgc tcggtggcca cccccgcgat ccggcccagg tcgcgcggcg
     2041 tgaggtccgc gaacagcggc acgttccgca ggaacagcac tcggtccatc gccgacaacg
     2101 tggccagcgt ctccatctgc ttccctcctt cggtggtggc cgcggcgaag gcggcgcatg
     2161 atcgcagcca cgggtcggcg tcgtccagga ggcgcaggag cgccctggcc cggtcgccgc
     2221 ggggctcggg ggctccctcc cagagcgcca cgatcggtcg cacgagggct cggggaccca
     2281 cggcctcgag catctcgagc gcgttcgcgc gctgctgcgc gtcccggctc tccaggttgg
     2341 ccgcggccag ctccacggcg ctcggatcac cggtgagggc cgcggcccgc agcgcgttgc
     2401 gggcgtgggt cagcgactcc tcccggacgg ccgaggccag cagccccatg cgcgggtcgc
     2461 cgtcgttccc cagggccctc cacaggccgt cgtaccggag cgcctcgccc gtctcccgct
     2521 cgacgtaggc acggagcagg tcggggtcgg cgtgccggat gtctcgcagg gcctcgaccg
     2581 cggcctcccg cacactcggg tggggatcct ggagagcctg gaggaccgcc cgggcggcgc
     2641 ccccggcctc gagcgacccg gccgcggcca ccgcggcgcg gcgcaccgac acatcgggat
     2701 ccgacatccg ctccaggacg atgtccggat ccgcgcccgt ctcggccagg gccgtcaccg
     2761 cagctcgtcg ccggtcgggc tccacggcca ccgccatctc caccagcagg tcgagcgcct
     2821 gctcgtcgcc cgaggcgacg agcgtggcgg ccgccctcgc cgcgatctcg ggatcgggat
     2881 cagtcaggag gggtcggagc gaggtgctcg cggccccacc tcccagggcc cgcatcgcct
     2941 gcacggcgct gccccggacc ccggcgtcgg ggtcatccag cagcgcctcg atcgcggcgg
     3001 cgtgcgaggg gccccccgcc ctccccacgc cccgagccgc ggccgcccgc acctccgcgt
     3061 cggggtccga gagggcatcg aggagggcgg ggagggcggg gggcgcgtcc aaccgcgaca
     3121 ggacctccat ggagatccgg cggacgacgg ggtcgcgggt ggacgcgccc cccagcgccg
     3181 cctgcaccgc ctcggcgtcc caggcccgcc cggtgaaggg ctcgtcctcg ctcccgaaga
     3241 cgtccgggtt gcccgcccgg agcgccacct cgagcgcctc gccgtacgcc cgcctggctc
     3301 tccacatcgc ccacgcggtg agacctgccg cggccacgcc cagggccgcc agggcacgac
     3361 ccgagaggac gcggtccgcg aggaccagca tcgcgcccga gagcaccacc cccgcctgca
     3421 gcggccccgc gtccatgaag ctcctgatcc tggccctggt ctcggagggg atcacgttgt
     3481 agagcgcctg ccacccggtc ccccacaccc cgttcaccca caccatctgg acgaaccgga
     3541 agcccacgag gatggcgaac ccagaggcgg cgaccagcac gccgaagccc accgcgtaga
     3601 tcgcggcgag cgccagaacc atcgccggga ccccgaaccg cgcgaagaga cggttcgcca
     3661 ccaggagcga gaggagcagc gccgccgcgt tcgacacggc catgaacccg ccgaggaacc
     3721 ccgccagctc ctccgtccga gggaaccgag cggttgccgc ctcggcgaac tggaggctca
     3781 gcgtgaagta cagcagcgcg aacagcatca gcgcgagcga cagccatcga aggagcgggg
     3841 ccgcgcgcac ctgtctgagc gcctcccccg cctcggccag gaccgacgcg cgacggcctc
     3901 gtcgccgccg gcggaccccg ggtccgacga ccgcccgtcc cagggcgaag accgtgatca
     3961 gcgcggccgc ccagatcagc aagaggttct cggtgccgag cagggccgcc agcggccccg
     4021 tgaccaggcc gcccgcgatc ccgcccagga tcagccccac cccgtagagc gggaacagcc
     4081 gtttggcctg cctcgtgtcg tggacgtcgc cggccaggcc ccacgagacc atgacctggg
     4141 tggtccacgc gatcatcatc acgacccaga ggaccgggta gagccaccgc tggtcgagca
     4201 gcagggtcgt ccgcgcggcg agcaggagga cggcgagccc gagcgggagc gtcgccagca
     4261 cccggcccgg atggggacgg gcaaggaccg cgctcatcgc ggccatcacg aagaaggtgg
     4321 ccacccccag cgccatgtag aggtacggga ggaagtgcgg gccgaaccgc gagaagaaga
     4381 gcgactcgac cgccgcgcct cccagggtcg ccccgaacag gcccacgagc atcacggcga
     4441 ggaatcggag gacgaggccg ccctcaccgg gacggaccct cagtgcagcc cggagccgga
     4501 tcatcgtacg attctcacat gccccgccca gccgaagacg cgatcgcggc cggccgggag
     4561 gcgctcgagc gtcacgcctg gcaggaggcg tacgagaacc tgtcggaggc ggaccgccgg
     4621 ggctcactcg ccgccgaggg gctcgccctc ctggcggagt ccgcctggtg ggccgcccgc
     4681 cccgacgacg tgatcgaggc cctggagcgg gcctacgccg cctacctcaa ggaggacgat
     4741 cgcagggagg cagcgatgat ggccttccgc gtcgccgagc agcacggcat gcgcatggcg
     4801 ggcccccagg cccagggatg ggcctcccga gcggccgagc tggtcgccga cgacccggac
     4861 gcccccgtcc acggttggct cgcctggatc caggggctca tgaccctcgc catgggcgga
     4921 gacgaggagg agtcgctggg ccactacgat cgagcgctcg agatcgcccg gcgtctcggg
     4981 gaccgggatc tcgaggcgat gagtctccac gacatgggcc acatcctctg ctcccggggg
     5041 caggtgaccg aggggctggc cctgatggat caggtcatgg tggcggcggt cggcggggag
     5101 ctcgacccca ccgccacggg ctacgtgtac tgcggcatga tcggtatctg ctcccgactc
     5161 ggcgactacg gccgcgcagc cgagtggacc gaggccacga tgcgctggtg cgagcgtcac
     5221 tcgatcacgg ggttcccggg ggtgtgtcgt gtccaccggg ccgagcttct gcggctgcgg
     5281 ggagccttgg agcaggcgga ggaggaggcc cggctggcct gcgacgagct gccgcgcttc
     5341 aacctctact cggggctggg tcacgcctgg tacgagatcg cggaagtccg ccgcaggatg
     5401 ggtgacgtcg ccgccgccga ggctgcgttc gccgcagcgc acgagcacgg gaagagtcct
     5461 cagccggggc tgtccctcct ccggctcgcc caggggcgcg tggaggcggc cgcggccggg
     5521 atcgcccagg ccctggccga ggccggggat gatcgacttg cccgcatgcg gcacctgccc
     5581 gcccaggcca tgatcgctct ggaggcgggg gacctggaga cggcggccgc ggccgccgag
     5641 gagctcgcag agatcgtgga ggggcacggc gcaccggcga tgcgcgcgac gctctcctcg
     5701 gtgcgggggg ccctgctcct ggcccgcggg gatgccgagg ccgcgctgcc ccacctccgc
     5761 gaggcgaggg acggctggag gggcctcgac gctccctatg agatcgccga ggtgcggctg
     5821 ctgctggcgc gagcctgccg ggcgaccggc gacgaggacg gggcgcggat ggaggccagg
     5881 gccgctcgga gcgccttcga gaccctcggg gcacggccgg ccgcggaggc cgcggcggag
     5941 ctcgtcggca cgcctaccgc ggccgagagg cccgagcggg tgcgccgggc cttcatgttc
     6001 accgacatcg agcgctcgac ggacctggtc ggcgtgatcg gcgacgaggc ctgggagagc
     6061 ctgctcgggt ggcacgatcg cacgctccgc tcgctcttcg cctcccacgg cggggaggtg
     6121 gcacaccaca cgggcgacgg gttcttcgtg tctttcgagg atccagcgtc ggccctggcg
     6181 tgcgcggtgg ccgtacagcg cgcgctcgca gagcaccgaa gaacccacgg cttcgccccc
     6241 cgggtccgca tcggcgtcca cacggccgag gcgacgaggc gcggacagga ctacggaggg
     6301 gtggaggtgc acaaggcggc ccggatcgcc gcggaggccc gcggcgggga gatcctggcc
     6361 agcgaggaga cggcaagggg gggcgggacg acggtctccg gagatccgcg aacggtctcc
     6421 ctgaaaggga tctcggagcc cgtacgggtc gtccccgtgc gctggcgaga cgggtaggcc
     6481 gttcgacctc atccgttcgg gggagtcctc acggccccct cgtggcgggg ctcacgctcg
     6541 tagcctcgg
//
LOCUS       DASUTZ010000201         3798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_585941, whole genome shotgun sequence.
ACCESSION   DASUTZ010000201 DASUTZ010000000
VERSION     DASUTZ010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585941"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1733)
                     /locus_tag="VF097_08815"
     CDS             complement(<1..1733)
                     /locus_tag="VF097_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262932.1"
                     /translation="MRLRYSRWDGSQDPLGPELSAAEVLEAMSDDLLEGAGPEAALRG
                     LARRGIRGRITGTDALRARLRDARRREQERLDLEGPLREVAEELEEILELERSALSLD
                     PEDDARMRESFLDALPRDPAGRIRELREYRFTDAVSQRRFDELMAKLAEQVLGAYFRQ
                     LAGGMRDLTPEHLARIKDMLAELNAMIEARDRGEPYDFQGFMERYGDMFPGSPRTLDE
                     LLEGMARRMAAMSRLMASLSPDQQRELRELAEQVMRDMDLAFEADRLGSHLAELFPDM
                     PWGDPVEGGGEGSMPLDATVGALERVHDFDELDRALQGDYPGASLDDLDEDAVRRTLG
                     EEAVADVRRLKRIEKVLEEAGLLSRKDGRLQVTPRGARRLGERALIKVFERLRRDREG
                     AHEARETGAGAEPTGATRPWRFGDPALIDVQRTVFNAVLREGPGASVSLRPDDFELIE
                     AETRTETATALLLDLSFSMPLRGHFLPAKRMALALHALIEGRYPHDRLYLVGFSDYAR
                     QLQPEDLTAPGFERVYGTNMHHAFHLAGRLLARHPRATRQVIMVTDGEPTAHLEGDEV
                     FFSWPPVRETIR"
     gene            complement(1738..3141)
                     /locus_tag="VF097_08820"
     CDS             complement(1738..3141)
                     /locus_tag="VF097_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6262933.1"
                     /translation="MSRPATIEALRETGYPDRSVKEELRDNLLARLRGGEELFPGVLG
                     FTDSVLPALERGILAGHDLILLGERGQAKTRLIRHLTGLLDEETPIVAGCEVNDHPFR
                     PICARCRALVAEHGGDTPVAWVGRDARYAEKLATPDTSVADLIGDVDPIKVAEGRHLG
                     DELTIHYGLVPRTNRGIVAINELPDLPERIQVALFNILEERDVQIRGYKVRLPLDLLL
                     VATANPEDYTHRGRIVSPLKDRFGTQVRTHYPDDPEDEIAIMRQEARPPASDIPVLVP
                     VFLEEVLGELTRELRESPHVNHRSGVSVRFSIGNLETLVAAALRRAARTGEAEAVPRP
                     CDLPAVLDASLGRIEFDTIEEGREEEILTRALASAILKVFRRRLAGFDLNPLLDRFDE
                     GLVVSTSDLTPAAHMLGQLDRVPGLAKLLSAVGAREESPGVAASALEFALEGLHLSRR
                     LNRDADGRGAYRYESAG"
     gene            3320..>3798
                     /locus_tag="VF097_08825"
     CDS             3320..>3798
                     /locus_tag="VF097_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262934.1"
                     /translation="MLAFSSDRGSGFNIFAVPAEGGDPVLLVGGEGEDHDPAWSPDGS
                     MLALARVTDRGRRSSIFLVEADGSGLRQLTSGPHLDRQPSWSPDGTEIAFVRAVPAQG
                     ISRIHVVRLGGGRRRLREPPEAGAVGIHDEQVRRPSREGPRERDARAVRRPRGAGVVG
                     "
BASE COUNT          495 a         1400 c         1357 g          546 t
ORIGIN      
        1 cggatcgtct cccgcaccgg cggccacgag aagaagacct cgtcgccctc caggtgcgcg
       61 gtcggctcgc cgtcggtgac catgatcacc tggcgcgtgg cccgcgggtg tcgggccagc
      121 agccgccccg ccagatggaa ggcgtggtgc atgttcgtcc cgtagacgcg ctcgaaccca
      181 ggggccgtca ggtcctcggg ctggagctgg cgcgcgtagt ccgagaaccc cacgaggtac
      241 agccggtcgt gcgggtaccg accctcgatc agggcatgga gcgccagcgc catccgcttc
      301 gccgggagga agtgtcctcg gaggggcatc gagaaggaga ggtcgagcag cagcgcggtg
      361 gccgtctccg tacgggtctc ggcctcgatc agctcgaagt cgtcgggcct cagcgagacg
      421 ctcgcgccgg gaccctcgcg cagcaccgcg ttgaacaccg ttcgctggac gtcgatcagg
      481 gccgggtccc cgaagcgcca gggacgcgtg gcgccggtgg gctcggcgcc cgcgccggtc
      541 tcgcgggcct cgtgggcccc ctcgcgatcg cggcgcagtc gctcgaacac cttgatcagc
      601 gcgcgctcgc ccagccgccg cgccccgcgc ggcgtcacct ggagtcggcc gtccttcctc
      661 gagaggagcc ccgcctcctc cagcaccttc tcgatccgct tcaggcgccg gacgtcggcc
      721 acggcctcct cccccagcgt ccgccgcacc gcgtcctcgt ccaggtcgtc cagcgaggcg
      781 cccggatagt cgccctggag cgcgcggtcg agctcgtcga agtcgtggac ccgctccaac
      841 gcgccgaccg tggcgtcgag cggcatcgag ccctcgcctc cgccctccac cgggtctccc
      901 cagggcatgt cggggaacag ctccgccagg tggctcccca ggcggtctgc ttcgaaggca
      961 agatccatgt cccgcatcac ctgctcggcc agctcccgga gctcccgctg ctggtcgggg
     1021 gagagcgacg ccatgagccg gctcatcgcg gccatccgcc gggccatccc ctccagcagc
     1081 tcgtcgagcg tccgggggct cccggggaac atgtccccgt accgctccat gaacccctgg
     1141 aagtcgtagg gctcgccgcg atcgcgggcc tcgatcatcg cgttgagctc ggccagcatg
     1201 tccttgatcc gcgcgaggtg ctcgggggtg aggtctcgca tcccgccggc cagctggcgg
     1261 aagtacgcgc cgagcacctg ctccgcgagc ttcgccatca gctcgtcgaa cctccgctgc
     1321 gacaccgcgt cggtgaagcg gtactcgcgg agctcgcgga tcctcccggc cgggtcccgc
     1381 ggcagcgcgt cgaggaacga ctcccgcatc cgcgcgtcgt cctccgggtc gagggagagc
     1441 gcgctgcgct cgagctccag gatctcctcg agctcctcgg ccacctcgcg caggggtccc
     1501 tcgaggtcga gccgttcctg ctcccggcgc cgcgcgtcgc ggagtcgggc ccggagggcg
     1561 tcggtgccgg tgatgcggcc ccggatcccc cggcgggcca ggccccggag ggccgcttcg
     1621 ggccccgcgc cctccagcag gtcgtcggac atcgcctcga gcacctcggc cgccgacagc
     1681 tccggcccga gcgggtcctg gctcccgtcc cagcgcgagt accggaggcg cacgccctca
     1741 gcccgccgac tcgtagcggt acgcgccgcg gccgtccgcg tccctgttca gccgcctgga
     1801 caggtggagg ccctccaggg cgaactcgag ggccgaggcg gccacgcccg ggctctcctc
     1861 tcgggctccg actgccgaga gcagcttggc cagcccgggg acccgatcga gctggcccag
     1921 catgtgcgcc gccggcgtca ggtccgacgt cgagaccacg aggccctcgt cgaaccgatc
     1981 caggagcggg ttcagatcga agccggccag ccgccgccgg aacaccttga ggatcgcgga
     2041 ggccagcgcg cgggtcagga tctcctcctc gcggccctcc tcgatcgtgt cgaactcgat
     2101 cctccccagc gaggcgtcga ggacggccgg cagatcgcag ggccggggga ccgcctcggc
     2161 ctcccccgtc cgggccgcac gccgcagagc ggccgccacg agcgtctcca ggttcccgat
     2221 cgagaacctc acggagactc cggagcggtg gttcacgtgg ggcgactcgc ggagctcgcg
     2281 ggtaagctcg cccagcacct cctccaggaa gacgggaacc agcaccggga tgtccgaggc
     2341 cggcggcctc gcctcctgcc gcatgatcgc gatctcgtcc tcgggatcgt ctgggtagtg
     2401 ggtgcggacc tgggtcccga agcggtcctt cagcggagac acgatccgcc cgcggtgcgt
     2461 gtagtcctcg ggattggccg tcgccacgag caggaggtcg agcggcaggc ggaccttgta
     2521 cccgcggatc tgcacgtcgc gctcctcgag gatgttgaag agggccacct ggatccgctc
     2581 ggggaggtcc ggcagctcgt tgatcgccac gatcccacgg ttcgtgcggg gtacgagccc
     2641 gtagtggatc gtgagctcgt cgcccaggtg tcggccctcg gccaccttga tcgggtccac
     2701 gtcgccgatg aggtcggcca cgctcgtgtc gggggtggcg agtttctccg cgtagcgcgc
     2761 gtcacggccg acccaggcca ccggcgtgtc gcccccgtgc tcggctacga gcgcccggca
     2821 ccgggcgcag atcgggcgga aggggtggtc gttgacctcg cagcccgcaa cgatgggggt
     2881 ctcctcgtcg aggagcccgg tgaggtggcg gatcagcctg gtcttcgcct gaccgcgctc
     2941 accgagcagg atcaggtcgt ggccggcgag gatgcctcgc tccagagcgg ggaggaccga
     3001 gtcggtgaag ccgaggacgc cggggaagag ctcctcccct ccgcggagtc gggccaggag
     3061 gttgtcgcgg agctcctcct tcacgctgcg gtcgggatac cccgtctcgc ggagggcttc
     3121 gatcgttgcg ggtcgactca tgggccccag cgtaccctgg ggggagagga gagagggttc
     3181 ccggctcgag cgtcctccgc tatcctgtcg cctcgcatcg aagggagacg tcacgagtcg
     3241 ccgccgcgcc gtcgtgctgg tcccgctcgc cgccgccacc ttggccgccc ccgcgccctc
     3301 gccggcgacc gatgctccga tgctcgcctt cagcagcgat cggggatccg ggttcaacat
     3361 cttcgccgtc cccgcagagg ggggtgaccc cgtgctgctg gttggtgggg agggcgagga
     3421 ccacgatccc gcgtggtccc ccgacgggag catgctggcc ttggcccggg tcacggatcg
     3481 aggccgcagg tcgtcgatct tcctggtcga ggccgatgga agcggcctgc ggcagctcac
     3541 ctccggaccg cacctcgacc gccagccgtc gtggtcaccc gacggaacgg agatcgcgtt
     3601 cgtccgggcc gtgccggcgc agggcatctc ccggatccac gtcgtgaggc tggggggcgg
     3661 ccggcggcgg ctccgtgagc cgccggaggc cggcgccgtc ggcatccacg acgaacaggt
     3721 ccggcgtccg tcccgggagg gcccgcgaga acgcgacgcg cgagccgtcc ggcgaccacg
     3781 cggggccggc gtcgtggg
//
LOCUS       DASUTZ010000202         6405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_586527, whole genome shotgun sequence.
ACCESSION   DASUTZ010000202 DASUTZ010000000
VERSION     DASUTZ010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6405
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586527"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="VF097_08830"
     CDS             <1..785
                     /locus_tag="VF097_08830"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEX6262935.1"
                     /translation="ILAYSCSSRDLPMANKKKKRKKLAPGAPTSAPAEEREARKEAAR
                     QDREKAIRSYRRKRLVKRVGVVALVVAVVGGGGFLVVNAMRSSEQLDETIAALAREVG
                     CGEILTDDAGDLVDEGAGHVDQPPQYQNVPATSGPHFTQWLPPGERVYDVPFDPAFEF
                     RAVHNLEHGYIIMYYQQDGEGALDPGIVEDLQTMARSQPKVMIAPYPNLQEDENLVLV
                     AWNRAQRCDVSGASAGDVREVAEYFMDQFRGDGGEAPEPNAA"
     gene            complement(782..2299)
                     /locus_tag="VF097_08835"
     CDS             complement(782..2299)
                     /locus_tag="VF097_08835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEX6262936.1"
                     /translation="MVARRLLLVSILAAALAVAPLAGAADRFTFHGSGWGHGIGMGQY
                     GAFGLAKKGWKAPRILKHYYTGISVERRDPPRDVFRVGLLQYRERITVSAQTGPFDLA
                     LSNGTHIETVTQGRTRVITVQGGEFRIETASGQLVGGRLWGGANRHLQLLRADGSVIR
                     VAEWGRRAGRGMLELRIADQGSAHLVAVLDPEEYLYGLGEVPSSWPMAALKAQAIAGR
                     TYAYQRAAGGRTGCACHILGDTRDQAYVGWDKEVGVDGDRWVRAVDETARRVALHGGA
                     PITTFYSSSSGGYTESVENVWGGAPQPYLKGVCDPGDFVSSNPNRTWEVTLSSGDVAA
                     RLRSALGWSVADVRSIKAVRRGVSGRLVEVRVRGRSSGGGSFSRTTSGWSLRGALGLK
                     DTRVWVNADRSVTGAIRKEYDRILCRPGLATSRQRMAAGGVWQRFQRGRIFRKGIAAW
                     IRKPILPVYLDRGGPSGPLGYPTSRQKKLKDGRVRARFEGGVVTCRPDGGCSVRT"
     gene            complement(2303..3991)
                     /locus_tag="VF097_08840"
     CDS             complement(2303..3991)
                     /locus_tag="VF097_08840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEX6262937.1"
                     /translation="MTRDPKLAALRAALAAVVVAGLLAAPAGAADGFRFNGSGWGHGI
                     GLGQYGAFGLAQEGWGPAKIVRHYYTNTSVQRRDPPKGRFRVGLLQGRQSVSLAVQRG
                     RAVLRVGKAVVERIRAGATRRILIVNGRYRVKRPNGSVVKNRLWGSAKKPLEVQLKNG
                     GVVRVPEWGHSAGRGFLRLKIVGKRSAHLVAVVPAEQYLYGLGEVPSSWPMAALKAQA
                     IAARSYAYVVVGFGRTGCACDIYASTRDQAYVGWDKESGTAGGRWVKAVNGTSRRVAI
                     HGGAVIQGLYSSSSGGHTENVENVWGGGAVGYLKGVCDPGDWTTANPYRLWSTSFTPR
                     GLASRLGRPNGMVRVTAFNVNKRGVSGRVVTATIKGKRGNGSTTSWSTSGWNLRGRLG
                     LRDTRFWVNQDRQITGKIREAYDELDCRPGLPMSNQKKISGGRWQRFEDGRIYHHAGR
                     KRETWLRGPVLDAYLSLGGHGGILGRPWSYGAQAGGVRARFDGGAIYHHEAAGAHEVH
                     GPVLAAYLSHGGPASDLGFPTTDVVSEADGTLVSTFQGGTISCPPQAGCTVTPA"
     gene            complement(4053..5063)
                     /locus_tag="VF097_08845"
     CDS             complement(4053..5063)
                     /locus_tag="VF097_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEX6262938.1"
                     /translation="MKAVVLVGGEGTRLRPLTETRPKPLIPFMGRPFLHHLLDLLARH
                     GIEEAILSSPHLEEEFRAFLKERGGAPRITWITEETPLGTCGAIASAREHLEETFLVC
                     NGDILTDLDLSELVAFHGRREATGTIALTPVEDARPYGLVETDDESRILAFREKPSEP
                     IAADVNAGTYVLEPRALDGVPQGVPVSIERETFPGLIARGEPLYAFPSDAYWRDLGTP
                     ESYLAAHLDALEGRVRDVVAGRPHLGPGVVVEPGATVEGTVVLGRGARVARGAHVSRS
                     VLHEEALVAERAEVSGSILGPRSEVGADAVVEDAVLGDGARVAAGAAVHGERVGPERS
                     VS"
     gene            complement(5060..5947)
                     /locus_tag="VF097_08850"
     CDS             complement(5060..5947)
                     /locus_tag="VF097_08850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6262939.1"
                     /translation="MTADLAVVVVNFNAGEHLRSCVASVYAAAGDLDLDVVIVDNASR
                     DGSAGAAAAAHPSIRVINNRVNRGFAAAANRGFAETQAPFIFLLNPDAVISGGRLDAF
                     VKVAKERPRVGALGCLVRNPDGSLQPSARKVPGIGEGLAHAFLGRVAPDNRWSRSYTM
                     ADWDRASEREVEWVSGSAMLVRREAVDAVGGFDEGYFMYVEDVDLCTRLRKRGWSALF
                     TPELEVTHEVGVSTRSHPRRMAYEHSRSIYRYFSTHVATGPGTVLRPLARATLWARAA
                     LIARRSPEDRVAGPRRRPR"
     gene            complement(5944..>6405)
                     /locus_tag="VF097_08855"
     CDS             complement(5944..>6405)
                     /locus_tag="VF097_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262940.1"
                     /translation="GPVTEHRHTDPLDMYYEPPPPPGEKRRTLMLLGLIIGSVVLAAV
                     AQLTLKYGMNQVGAIGTSDLGSPVATALRVFRQPAVLGGFALFGLSAVLWLVVLSRTS
                     LSFAYPFAGITYVLILIFDRVVLGEPVTALRWTGVFLIIGGLVLISRTAPT"
BASE COUNT          943 a         2373 c         2213 g          876 t
ORIGIN      
        1 cgatccttgc gtatagttgc tcgtcgaggg atctccccat ggcgaacaag aagaagaagc
       61 ggaagaagct cgcgcccggc gcgcccacgt cggcccccgc cgaggagcgg gaggcccgga
      121 aggaggccgc tcgccaggac cgcgagaagg cgatccggtc ctaccgtcgg aagcgtctgg
      181 tgaagcgcgt cggcgtcgtg gcgctcgtcg tcgccgtcgt gggcggggga gggttcctcg
      241 tcgtcaacgc gatgcgatcg agcgaacagc tcgacgagac gatcgccgcg ctggccaggg
      301 aggtcggctg cggggagatc ctcaccgacg acgccgggga cctggtcgac gagggcgctg
      361 ggcacgtcga ccagccgccc cagtaccaga acgtcccggc cacgtccgga ccccacttca
      421 cgcagtggct gccccccggc gagcgcgtct acgacgtgcc cttcgatccg gcgttcgagt
      481 tccgcgccgt ccacaacctc gagcacggct acatcatcat gtactaccag caggacgggg
      541 agggggccct tgatcccggg atcgtcgagg acctgcagac catggcccgc agccagccca
      601 aggtgatgat cgcgccctac ccgaacctcc aggaggacga gaacctcgtg ctcgtggcct
      661 ggaaccgggc ccagcggtgc gacgtctccg gggcctccgc cggcgacgtc cgcgaggtcg
      721 ccgagtactt catggaccag ttccgcggcg acgggggcga ggcccccgag cccaacgcgg
      781 cctaggtccg gaccgagcag cccccgtccg gtcggcaggt gacgacgccg ccctcgaacc
      841 gcgcccgcac gcgcccgtcc ttcagcttct tctgacggga cgtcggatac ccgagcggcc
      901 cgctcggccc cccccgatcg aggtagacgg ggaggatcgg cttgcggatc cacgccgcga
      961 tccccttacg gaagatccgt ccccgctgga accgctgcca cacgccgccg gcggccatcc
     1021 gctgacggct cgtggccagc ccggggcggc acaggatccg gtcgtactcc ttgcggatcg
     1081 cgccggtgac gctgcggtcg gcgttcaccc agacccttgt gtccttgagg cccagggcgc
     1141 cgcgcaggct ccatcccgag gtcgtcctcg agaacgaccc tccgcccgac gagcgacccc
     1201 gcacccggac ctccacgagc cggcccgaga cccctcgccg caccgccttg atcgatcgga
     1261 cgtcggccac cgaccatccg agggcggacc gcagacgcgc ggcgacgtcg ccggacgaga
     1321 gcgtcacctc ccaggtccgg ttggggttcg aggagacgaa gtcccctgga tcgcacacgc
     1381 ccttgaggta gggctggggc gccccgcccc agacgttctc cacgctctcg gtgtacccgc
     1441 cactcgagga cgagtagaac gtcgtgatcg gagcgccacc gtgcagcgcc acgcgccggg
     1501 ccgtctcgtc gaccgcgcgc acccagcggt cgccgtcgac accgacctcc ttgtcccacc
     1561 ccacgtaggc ctggtcccgc gtgtcgccca ggatgtggca cgcgcacccg gtccggccgc
     1621 cggccgcccg ctggtacgcg taggtgcgcc cggcgatcgc ctgggccttc agcgcggcca
     1681 tgggccacga ggaggggacc tcccccagcc cgtagaggta ctcctccggg tcgagcacgg
     1741 ccaccagatg ggcgctgccc tggtcggcga tccggagctc gagcatcccg cgcccggccc
     1801 ggcgccccca ctcggccact cggatcaccg agccgtcggc acggagcagc tggaggtgcc
     1861 tgttcgcccc gccccagagt cgaccgccca cgagctggcc ggaggccgtc tcgatccgga
     1921 actcgccgcc ctgcaccgtg atcaccctgg tgcgaccctg tgtcacggtc tcgatgtgcg
     1981 tgccgttcga gagcgccagg tcgaagggtc cggtctgggc gctgacggtg atccgttccc
     2041 gatactggag cagtcccacc ctgaaaacgt cgcgaggcgg atcccgccgc tcgacgctga
     2101 tgcccgtgta gtagtgcttg aggatccggg gggccttcca tcccttcttg gccagaccga
     2161 aggctccgta ctgacccatg ccgatcccgt gcccccatcc cgatccgtgg aaggtgaagc
     2221 ggtcggcggc gcccgccagc ggcgcgaccg ccagagccgc cgccaggatc gagacgagga
     2281 ggaggcgccg cgcgaccatc gctcaggcgg gggtcaccgt gcagccggct tggggcgggc
     2341 acgagatcgt gcccccctgg aacgtagaga cgagcgtgcc gtcggcctcc gagacgacgt
     2401 cggtcgtggg gaagccgagg tcggaggcgg gcccgccgtg gctcaggtag gccgcgagga
     2461 cggggccgtg gacctcgtgg gcacccgcgg cctcgtggtg gtatatcgct cccccgtcga
     2521 accgcgcgcg gacgcccccg gcctgggcgc cgtaggacca gggccgtccc aggatcccgc
     2581 cgtggccccc cagcgagagg tacgcgtcga ggacggggcc gcgcagccac gtctcgcgct
     2641 tccgcccggc gtggtggtag atccgaccgt cctcgaaccg ctgccaacgc ccgccactga
     2701 tcttcttctg attgctcatg gggagcccgg ggcggcagtc gagctcgtcg taggcctccc
     2761 ggatcttccc ggtgatctgg cgatcctgat tcacccagaa gcgcgtgtcc ctgaggccga
     2821 gccgaccccg caggttccac ccgcttgtgc tccaggacgt cgtggagccg ttgccgcgct
     2881 tgcctttgat cgtggccgtg acgacccgcc cggagacccc gcgcttgttc acgttgaagg
     2941 cggtgacgcg gaccatgccg ttcgggcggc ccagccgcga tgccaggccc cgcggcgtga
     3001 agctcgtgga ccagaggcgg taggggttgg ccgtggtcca gtccccgggg tcgcagacgc
     3061 ccttcagata gccgacggcg ccaccgcccc agacgttctc gacgttctcc gtgtgcccgc
     3121 cgctggagga ggaatacagc ccctggataa ccgcaccgcc gtggatcgcc accctcctcg
     3181 aggtgccgtt cacggccttc acccatctgc ctcccgccgt tcccgactcc ttgtcccatc
     3241 cgacgtacgc ctggtcccgc gtgctcgcgt agatgtcgca cgcacatccc gtgcgcccga
     3301 agccgacgac gacgtacgcg taggagcggg cggcgatggc ctgggccttc agggcggcca
     3361 tgggccacga ggatggaacc tcgcccaggc cgtagaggta ctgctcggcg ggcacgacgg
     3421 ccacgaggtg cgcgctccgc ttcccgacga tcttcagacg gaggaagccg cggcccgcgc
     3481 tgtgccccca ctcggggacg cggaccaccc cgccgttctt cagctggacc tcgagcggct
     3541 tcttggccga cccccagagg cggttcttga ccacgctgcc gttcggacgc ttgacgcggt
     3601 accggccgtt cacgatgagg atccggcgtg tggccccggc gcggatccgc tccacgacgg
     3661 ccttccccac tcggagcacc gccctccccc tctgcaccgc gagcgagacc gactgccgac
     3721 cctggaggag tcccacccgg aaccggccct tgggcgggtc gcggcgctgg acgctggtgt
     3781 tcgtgtagta gtgccggacg atcttggcag ggccccatcc ctcctgggcc agcccgaagg
     3841 cgccgtactg accgagcccg atcccgtggc cccagcccga gccgttgaag cggaagccgt
     3901 ccgccgctcc ggccggggcc gcgaggagac ccgcgacgac gaccgcggcg agcgcggctc
     3961 ggagcgcggc gagcttcggg tccctggtca tggtgtgttg gtcacatcgt cggagcgggc
     4021 accccgattt agccccatct gggggccgat gtctaggaga ccgagcgctc cggccctacg
     4081 cgctcgccgt ggacggcggc cccggcggcg accctggctc cgtcgcctag gaccgcgtcc
     4141 tcgaccacgg cgtccgcgcc cacctcgctc cggggcccga ggatgctgcc cgagacctcg
     4201 gcccgctcgg ccacgagcgc ctcctcgtgc agcacgctgc gcgagacgtg ggccccgcgc
     4261 gccacacgag cgccgcgccc gagcaccacc gtcccctcaa ccgtcgcccc gggctcgacc
     4321 acgaccccgg gcccgaggtg gggccgcccc gcgacgacat cccggaccct cccctcgagc
     4381 gcgtcgaggt gggcggcgag atacgactcc ggcgtcccca ggtcgcgcca gtaggcgtcg
     4441 gaagggaacg cgtagagagg ctcgccccgg gcgatgaggc ccgggaacgt ctcgcgctcg
     4501 atcgagaccg ggaccccctg ggggacgccg tcgagcgcgc ggggctcgag gacgtacgtc
     4561 cccgcgttga cgtcggccgc gatcggctcg ctcggcttct cacggaacgc caggatccgg
     4621 ctctcgtcgt cggtctccac gagcccgtac ggtcgggcgt cctccaccgg ggtgagcgcg
     4681 atcgtccccg tcgcctcccg ccggccgtgg aaggccacga gctccgagag gtcgaggtcc
     4741 gtgaggatgt cgccgttgca gacgaggaac gtctcctcga ggtgttcgcg agctgaggcg
     4801 atcgcgccgc aggtcccgag cggcgtctcc tcggtgatcc acgtgatccg gggcgcccct
     4861 ccgcgctcct tcaggaacgc ccggaactcc tcctcgaggt ggggcgagga gaggatcgcc
     4921 tcctcgatcc catgccgagc cagcaggtcg agcagatggt ggaggaaggg cctgcccatg
     4981 aaggggatca gcggcttggg ccgcgtctcg gtgagcggcc gaagccgcgt cccctcgcca
     5041 ccgacgagga cgaccgcctt caacgaggcc gtctccgggg gccggccacc cgatcctcgg
     5101 ggctgcggcg cgcgatcagc gctgcccggg cccagagcgt cgcgcgggcc agcggtcgca
     5161 ggaccgtacc gggcccggtg gcgacgtgcg tcgagaagta gcggtagatg ctccgggaat
     5221 gttcgtaggc catgcggcgg ggatggctcc gcgtcgagac ccccacctcg tgcgtcacct
     5281 ccagctcggg cgtgaacagc gccgaccagc cccgcttccg aagacgcgta cagaggtcca
     5341 cgtcctcgac gtacatgaag tagccctcgt cgaacccgcc gacggcgtcg acggcctccc
     5401 gccggaccag catcgccgac ccggacaccc actccacttc tcgctcggag gcccggtccc
     5461 agtcggccat cgtgtacgag cgggaccagc ggttgtccgg agccaccctc cccaggaaag
     5521 cgtgggccag tccctcgccg atcccgggga ccttccgggc ggagggctgg agcgacccat
     5581 cggggttgcg caccagacag cccagcgcgc ccacccgcgg ccgttccttc gccaccttga
     5641 cgaaggcgtc caggcggccg ccactgatca ccgcgtcggg gttgaggagg aagatgaacg
     5701 gcgcctgggt ctcggcgaac ccgcggttgg ccgcggcggc aaacccgcgg ttcacccggt
     5761 tgttgatgac ccggatcgac gggtgagccg ccgcggccgc ccccgcgctc ccgtccctcg
     5821 aggcgttgtc gacgatcacg acgtcgaggt cgaggtcgcc cgccgccgcg tagacggagg
     5881 ccacgcagct ccgaaggtgc tcgccggcgt tgaagttgac cacgacgacg gcgaggtcgg
     5941 cggtcacgtg ggggccgtcc gggagatcag gaccaggccg ccgatgatga ggaacacgcc
     6001 cgtccagcgc agggccgtga ccggctcgcc cagcacgacc cggtcgaaga tcaggatcag
     6061 cacgtaggtg atcccggcga acgggtacgc gaacgagagc gaggtcctcg acagcacgac
     6121 gagccagagg actgccgaca gaccgaagag cgcgaagccc ccgaggaccg cgggctgacg
     6181 gaacacgcgg agggccgtgg ccacggggga cccgaggtcg gacgtcccga tcgcgccgac
     6241 ctggttcatc ccgtacttca gggtgagctg ggcgacggcc gccaggacga ccgagccgat
     6301 gatcagtccg agcagcatca aggtcctcct cttctccccc gggggcgggg gaggctcgta
     6361 gtacatgtcc agcggatcgg tgtgccggtg ctccgtcaca ggtcc
//
LOCUS       DASUTZ010000203         6415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_327961, whole genome shotgun sequence.
ACCESSION   DASUTZ010000203 DASUTZ010000000
VERSION     DASUTZ010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6415
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327961"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..749)
                     /gene="rpiB"
                     /locus_tag="VF097_08860"
     CDS             complement(156..749)
                     /gene="rpiB"
                     /locus_tag="VF097_08860"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX6262941.1"
                     /translation="MSERRSPEEVRALVATVVDAVAGEAHPVPREDEPAAPSPEGTPS
                     LADVAVGADHGGFHLKQRIAAELRDHGFSVQDCGTEGSDSVDYPDYAHEVARLVAEGA
                     CRWGIVVDGAGIGSAITANKVPGVRAATCWDVSSARNSREHNHANVLALGAGLVGEAL
                     ALQIVQAWLSTGWGGGRHARRVSMIDKIERTYARTTA"
     gene            complement(746..1519)
                     /locus_tag="VF097_08865"
     CDS             complement(746..1519)
                     /locus_tag="VF097_08865"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX6262942.1"
                     /translation="MMNPAQASLDRRGPLPLYFQLREALLRDVRDRGLQPGDRLPTEA
                     ELERRYGVSRSTIRQAMNELAAEGLVHRIQGKGTFLGTPKIQHVPVLTSYSELLRSQG
                     YRPSHRLLVSATRPAPSEVAEALGIDEGETCRFLRRLFLADEDPVGLAETWLPQDVLG
                     EHDGLLERGAIEDGSLYELLQGEPIALRLHRAVETINPGVAEDGEAGLLGCEQGSPLL
                     VIHRITFTPQERPIEWTRLLFAGGRYEYRVEMHRPGGSS"
     gene            complement(1581..2039)
                     /locus_tag="VF097_08870"
     CDS             complement(1581..2039)
                     /locus_tag="VF097_08870"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEX6262943.1"
                     /translation="MRYFAYGANMHPREMAKTSPTATVLGPGLVEGFRLTFGVYSDRW
                     KGGAANIMPAEGARCWGVVWDVPEDDVAKLDTFAGHPTYYRHDDVVVHVGGSLETCTT
                     YRVAHQGGYVRPDEAYLQVLRAAIREQGLPVEALDLLERAASLPYPRIDS"
     gene            complement(2084..2755)
                     /gene="uppS"
                     /locus_tag="VF097_08875"
     CDS             complement(2084..2755)
                     /gene="uppS"
                     /locus_tag="VF097_08875"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX6262944.1"
                     /translation="MDGNGRWARERGLPRTAGHEQGERALYDVVDGALEIGLRHLTVF
                     AFSTENWRRPPSEVRFLMGFNRRIIHSRRDELHQRGVRIRFLGRRGRPLPRSVLREME
                     EAERMTARNDRMTFTIALNYGGRLEVVDAVRRLLEDHDAGRLRGRLTERSIAARLYDP
                     ELPDPDLMIRTSGEQRLSNFLLWQSAYTELWFTPVLWPDFTREHLVEAIREYQRRERR
                     FGGVT"
     gene            complement(2800..3549)
                     /gene="recO"
                     /locus_tag="VF097_08880"
     CDS             complement(2800..3549)
                     /gene="recO"
                     /locus_tag="VF097_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014375434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX6262945.1"
                     /translation="MPLYKAQGIVLGSFKLGEADKILTILTQEEGKVRAVAKGIRRTK
                     SRFGARLEPFTHVDLLLYRGRNLDTVTQAEILAAFRRIREDYALFAAGEAMLEAADKV
                     AEEHERNVRLFLLLRKGLQALDAGPADPAAVAEAFLLKLLGLSGFGPILGACAACGGG
                     PVTRFSNEQGGSVCEACRDRDARKASPEALAWLSALAEGDLDEAGDATPAPRVRGEAR
                     ALLYGFTEHHLERRMRSFPLLARVEPRAVLP"
     gene            3643..6177
                     /locus_tag="VF097_08885"
     CDS             3643..6177
                     /locus_tag="VF097_08885"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEX6262946.1"
                     /translation="MSSIRSLRRYASTVAVTVFTAVSVAGLPALAHVGGTVGHLWTKH
                     IRSKTDARYFKKAQLRADDGVVNQAGDPVHWTKLRGVPAGFADGVDDTGGGGPATDVQ
                     CAGCVDTSDLANGTVSIPKLDFDPATQAELDSHAGSGDHDGRYFTEAELSTSDGTPPN
                     GGSNRVHWDNLAGVPAGFADGVDDTGGGGSGWALTGNAGTNPATDFLGTTDAQAMSIR
                     VNNARALRIEPGSTPNLIGGHSTNSATAGVVGAAIGGGGAGGTANRVTDDYGTVGGGQ
                     DNQAGDASGGTADRSWATVAGGLSNQARGQGSAIAGGGVNIAGGFTSFVGAGQANVVS
                     ASEAVIGGGFSNTASGTSVRSVIGGGEFNNLNATHTTIGGGLGNSVTAAYGTIAGGGR
                     STPADPATGNRVTDDYGTVGGGGGNRAGDAAGTTTDARFATVAGGNENVASGFIASAG
                     GGRLNEASGTGATIGGGFDSTASASNATVGGGFRNAASADNATIAGGIENVASGAWAT
                     VAGGSSNIASAEGAAIGGGGSLPNPNRVTDRSGTISGGEGNLAGDDDADIADASWATV
                     GGGFENSAEASFATAGGGHSNQATGSGATIAGGQLNDASGTNSTVAGGAVNLAQGSWS
                     VVPGGLSNEAVGDHSLAAGSGAVASHEGSFVWADSSIPDFLSTAANEFSVRSTGGARF
                     VSAVDGSGTPTAGVVLAPGAGSWSSLSDRDAKTALAPADTRAVLNQVASLPISTWSYE
                     SQDEGIRHIGPMAQDFHAAFGVGEDPRYIGTLDADGVALAAIQGLNDVVEKQERTIES
                     LRDRVDGSTPSSGPSVWSLVVAAIGLIGLGAGIGIGLAGRRRPALA"
BASE COUNT          951 a         2258 c         2336 g          870 t
ORIGIN      
        1 gtagctgatg cggtgggcgc cgttgtccac gatggcccgg accttgtccg ggacgtgggc
       61 cgcgcaggag ccgcggcagt cgggacagcg ctcggccggg gccagcgcgg ccagcacctc
      121 gctggtgacg gcctcgatca ggtcctcgcg cgcgctcatg ccgtcgtcct cgcgtaagtc
      181 cgctcgatct tgtcgatcat cgacacgcgc cgcgcgtgcc ggccaccgcc ccagcccgtg
      241 gagagccagg cctgcacgat ctgcagggcc agggcctcgc ccacgaggcc ggcgccgagc
      301 gccagcacgt tcgcgtggtt gtgctcgcgg ctgttccgcg cggacgagac gtcccagcag
      361 gtagcggcgc gcacgccggg caccttgttg gccgtgatgg cggatccgat ccccgcgccg
      421 tccaccacga tcccccaccg gcacgcgccc tcggccacca gccgcgccac ctcgtgcgcg
      481 taatccgggt agtccacgga gtcggacccc tccgtcccgc agtcctggac gctgaaccca
      541 tggtccctga gctcggccgc gatccgctgc ttcaggtgga agcccccgtg gtcggcaccg
      601 acggccacgt ccgccagcga cggggtcccc tcgggcgacg gagcggccgg ctcgtcctcg
      661 cgcggcaccg gatgggcctc gcctgccacc gcgtccacca ccgtggccac gagcgcccgg
      721 acctcctcgg ggctccgccg ctcgctcacg acgacccccc cggccggtgc atctcgaccc
      781 ggtactcgta cctgccgccc gcgaacagga ggcgcgtcca ctcgatcggc cgttcctggg
      841 gcgtgaaggt gatccggtgg atcacgagga gcgggctccc ctgctcgcac cccagcagcc
      901 cggcctcccc gtcctcggct acgcccggat tgatcgtctc gacggcccgg tggaggcgca
      961 gggcgatcgg ctcgccctgc aggagctcgt agagcgagcc gtcctcgatc gcgccccgct
     1021 cgagcaggcc gtcgtgctcc cccaggacat cctgcgggag ccaggtctcg gcgaggccga
     1081 cggggtcctc gtcggccagg aagagccgcc gcaggaagcg gcaggtctcg ccctcgtcga
     1141 tccccagggc ctcggccacc tcggacggcg ccgggcgggt ggccgagacg aggaggcggt
     1201 gtgaggggcg gtacccctgg ctgcggagta gctccgagta cgaggtcagc accggcacgt
     1261 gctgaatctt cggggtgccc aggaacgtgc ccttgccctg gatccggtgc acgaggccct
     1321 cggcggcgag ctcgttcatc gcctggcgga tcgtggatcg ggagacgccg tatcgccgct
     1381 cgagctcggc ctcagtgggg aggcgatccc cgggctggag gccccgatcg cgcacgtcgc
     1441 gcaggagggc ctcacgcagc tggaagtaga gggggagcgg cccccgccgg tcgagcgagg
     1501 cctgtgcagg attcatcatg atgatatgtc atcatcctct cccgtcgtgc ggctgtcaag
     1561 gaccccggtt gaggatggga tcagctgtcg atgcgggggt acggcagcga ggctgcccgc
     1621 tccagcaggt ccagagcctc caccgggagg ccctgctcgc ggatcgccgc ccggaggacc
     1681 tggaggtagg cctcatcggg ccgcacgtag ccgccctggt gggcgacgcg gtaggtcgtg
     1741 caggtctcga gggatccccc cacgtgcacg accacgtcgt cgtgccggta gtaggtcgga
     1801 tggccggcga acgtgtcgag cttggcgacg tcgtcctcgg ggacgtccca aaccacgccc
     1861 cagcaccgag ctccctcggc gggcatgatg ttggcggccc ctcccttcca gcggtccgag
     1921 tacacgccga acgtcagccg gaagccctcc acgagccctg ggccaagcac cgtcgcggtg
     1981 ggagacgtct tcgccatctc ccgcgggtgc atgttcgcgc cgtaggcgaa gtaccgcaca
     2041 ggacgatgct agccccggcc cggggaatac cgcgaatcca gtctcaggtg acgccgccga
     2101 agcggcgctc acgcctctgg tactcgcgga tggcttcgac gagatgctct cgggtgaagt
     2161 ccggccacag cacgggggtg aaccagagct cggtgtaggc gctctgccaa aggaggaagt
     2221 tcgagagtcg ctgctccccc gaggtccgga tcatgaggtc gggatcgggg agctccgggt
     2281 cgtagaggcg ggccgcgatg gagcgctcag tgaggcggcc gcgcaggcga ccggcgtcgt
     2341 gatcctcgag cagccgacgg acggcgtcga ccacctcgag gcgccctccg tagttcaggg
     2401 cgatggtgaa cgtcatgcgg tcgttccggg cggtcatgcg ctccgcctcc tccatctcgc
     2461 ggaggaccga gcggggcagg ggccgccccc gccggccgag gaaccggatc cgcaccccgc
     2521 gctggtgaag ctcgtcccgc cggctgtgga tgatcctccg gttgaagccc atcaggaagc
     2581 ggacctcgga gggcggccgg cgccagttct cggtagagaa ggcgaagacc gtgaggtggc
     2641 ggagcccgat ctccagggct ccatccacca cgtcgtagag ggcccgctcc ccctgctcgt
     2701 ggccggccgt ccggggcagg cctcgctccc gggcccaccg gccgttgccg tccatgacga
     2761 tggccacgtg ggccgggagg cggtcgagat cgaggtccct cacgggagga cggcgcgcgg
     2821 ctcgacccgc gccaggagcg ggaagctgcg catacggcgc tcgaggtggt gctcggtgaa
     2881 cccgtagagg agggcccgag cctctcctcg gacccgcggg gccggggtcg cgtcgccggc
     2941 ctcgtccaga tcgccctcgg cgagcgcgct gagccaggcc agcgcctcgg gggaagcctt
     3001 ccgggcgtcg cggtcgcggc acgcctcgca cacggagccc ccctgttcgt tggagaagcg
     3061 ggtgacggga ccgccgccgc acgcggcgca ggcgccgagg atcgggccga agccggacag
     3121 ccccagcagc ttcagcagga aggcctcggc caccgcggcg ggatcggcgg gcccggcgtc
     3181 gagcgcctgc aggcccttcc ggagaaggag gaacagccgg acgttccgct cgtgctcctc
     3241 cgccaccttg tcggccgcct ccagcatcgc ctcccccgcg gcgaacagcg cgtagtcctc
     3301 gcggatcctc cggaacgcgg ccaggatctc cgcctgggtg accgtgtcca ggttgcggcc
     3361 ccggtagagg agcagatcaa cgtgggtgaa gggctccagg cgggccccga atcggctctt
     3421 ggtcctccgg atccccttgg ccacggcccg gaccttgccc tcctcctggg tcaggatcgt
     3481 gaggatcttg tcggcctccc ccagcttgaa ggagccgagc acgatcccct gggccttata
     3541 gagcggcatc gaccgcatcg tatcagcggg gatccgccgc cctaagcaag ccttcaccgc
     3601 ctgagggcat actcgcgcga cgcgacctcc aggaggtgca ggatgagctc catccgttcg
     3661 ctccgccgct acgcctcgac cgtggcggtg acggtcttca ccgccgtgtc cgtggccggc
     3721 cttccagcgc tggctcacgt cggcggaacc gtcggtcatc tctggaccaa gcacatccgc
     3781 tcgaagaccg acgccaggta cttcaagaag gcgcagctcc gggcggacga cggcgtcgtg
     3841 aaccaggccg gcgacccagt ccactggacg aagctcaggg gcgttcccgc cggatttgcg
     3901 gacggagtgg acgacacggg cggaggcggg ccggccaccg acgtgcagtg cgcgggctgc
     3961 gtcgacacct ccgacctcgc gaacgggacc gtctccatcc cgaagctcga tttcgacccg
     4021 gccacgcagg cggagctcga cagccacgcg ggatcgggcg accacgacgg gaggtacttc
     4081 accgaggcgg agctgtccac ctcggacggg acgccgccca atggaggatc gaaccgcgtg
     4141 cactgggaca acctcgcggg cgtcccggcg gggttcgccg acggggtcga cgacacgggt
     4201 gggggcgggt ccggctgggc gctcacgggg aacgccggca cgaacccggc cacggacttc
     4261 ctcggcacga cggatgccca ggccatgtcg atccgcgtga acaacgcccg ggcgctccgg
     4321 atcgagcccg gctccacgcc caacctcatc ggggggcact ccacgaacag cgcgaccgcg
     4381 ggcgtagtcg gcgcggcgat cggcggcgga ggcgccgggg gcacggcgaa ccgcgtgacc
     4441 gacgactacg gcaccgtcgg cgggggtcag gacaatcaag ccggcgatgc gagcggcggg
     4501 acggctgacc ggtcgtgggc gaccgtggcc ggcgggctct cgaaccaggc ccgcggccag
     4561 gggtcggcca tcgccggggg cggcgtgaac atcgccggcg ggttcacctc gttcgtcggc
     4621 gcggggcagg ccaatgtggt cagcgcctct gaggccgtga tcggcggtgg gttcagcaac
     4681 accgcgtccg ggaccagcgt ccggagcgtg atcggcgggg gcgagttcaa caacctcaac
     4741 gccacccaca ccacgatcgg gggcgggctc ggcaacagcg tgacggccgc ctacgggacg
     4801 atcgccggcg gggggcggtc gaccccggcc gaccccgcca cgggcaaccg cgtcacggac
     4861 gactacggca cggtgggagg cggcggcggg aaccgggcag gcgatgccgc cggaaccacg
     4921 accgacgctc gcttcgccac cgtcgccggc ggaaacgaga acgtcgcctc gggcttcatc
     4981 gcgagcgccg ggggagggcg acttaacgag gcatctggca ccggagccac catcggcggg
     5041 ggcttcgaca gcaccgcgtc agcctccaac gccacggtcg gcggcggctt tcggaacgcc
     5101 gccagcgccg acaacgcgac gatcgccggt gggatcgaga acgtcgcctc cggcgcatgg
     5161 gccacggtcg ccggagggag cagcaacatc gcgtccgcag agggcgctgc catcggaggg
     5221 ggtgggagcc tcccaaaccc gaaccgcgtg accgatcgct caggcacgat cagcgggggt
     5281 gaggggaacc tcgcaggcga cgacgatgcg gatatcgcgg atgcatcctg ggctacggtc
     5341 ggaggtggtt tcgagaacag cgccgaggcg agcttcgcga cggcgggggg tggtcactcc
     5401 aatcaggcaa ccggcagcgg ggcgacgatc gcaggagggc agctcaacga cgcgagcggc
     5461 acaaactcaa ccgtcgccgg cggcgccgtg aacctcgcgc aaggcagctg gtcggttgtt
     5521 ccgggcggcc tgagcaacga ggcggttggt gatcacagcc ttgcggcagg ttccggtgcg
     5581 gtcgcaagtc atgagggctc cttcgtctgg gccgattcct caatcccgga cttcctctcg
     5641 acggcggcca acgagttcag cgtgaggtca acggggggag cccggttcgt gtcggcggtg
     5701 gacgggagcg ggacgccgac ggccggcgtg gtgctggccc ccggcgcggg ctcgtggtcc
     5761 tctctctccg atcgagacgc gaagaccgcg ctggccccgg cggacacgcg cgccgtcctg
     5821 aatcaggtgg cctccttgcc catcagcacc tggagctacg aatcccagga cgaggggatc
     5881 cgtcacatcg ggccgatggc gcaggacttc cacgcggcct tcggcgtcgg cgaggatccc
     5941 cgctacatcg ggaccctgga cgccgacggt gtggccctgg ccgcgatcca gggcctcaac
     6001 gacgtcgtcg aaaagcagga gcggacgatc gaatcgcttc gcgaccgggt cgacgggagc
     6061 acgccctcgt ccggtccctc ggtctggtcc ctggtcgtgg ccgcgatcgg gctcattggc
     6121 ctgggagccg ggatcggcat cggcctggcc ggcaggagac gccccgccct cgcgtaggtc
     6181 ttggggccgg gggtcccggc atcaccggga cccccggccc tcccccgtgt ccctctcgac
     6241 cggtgcaatg gccggcgacg tgaccacgga tcgacggagg atcgaatgag cgagctgagg
     6301 cggatgacct tcggcgcggt gatcgggttc ctggtcgccg cgtccttcgg cgtgggactg
     6361 atctcgatgg ccgcctacag ggatggggag ccggtcgcgg ccgctcccgc ggccg
//
LOCUS       DASUTZ010000204         5167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_330282, whole genome shotgun sequence.
ACCESSION   DASUTZ010000204 DASUTZ010000000
VERSION     DASUTZ010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330282"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VF097_08890"
     CDS             <1..320
                     /locus_tag="VF097_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262947.1"
                     /translation="TVTETGTGGADSVTYSPDGGTPSDPPTVEITSDGQEVTVTVTNT
                     FIEVLGDITDRDTDTDTDVTTTEEGGTLAFTGAELLILALMALGLLVTGAPLLLLTRR
                     RDE"
     gene            complement(393..872)
                     /locus_tag="VF097_08895"
     CDS             complement(393..872)
                     /locus_tag="VF097_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX6262948.1"
                     /translation="MLSLAHPRIKRIPILMLLATLLLSLVAHGEAEAAPKAQRVERRL
                     MNQERRPKARATFRLDHGLSRIARRHSRRMARTGTLHHNPNLVDQVQGRRWQRLGENV
                     GVVGPTGDLRETLRLLHGAFMNSDAHRRNILYRPFRRVGVGLVRDDGLVWVTVVFLG"
     regulatory      complement(926..1003)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1075..1509)
                     /locus_tag="VF097_08900"
     CDS             complement(1075..1509)
                     /locus_tag="VF097_08900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX6262949.1"
                     /translation="MPREARDAERALLGVLVLVIALAAITGFTYPGPASRDEPLREAA
                     PGPQRPELFGVASWYGEPFRGRTSACGDPYDPDALTTAHRHLPCGTRLRVEHGGRGVT
                     VTVTDRGPFVPGRIIDLSRAAFARLAPLEEGLVEVRVVILSR"
     gene            complement(1555..2202)
                     /locus_tag="VF097_08905"
     CDS             complement(1555..2202)
                     /locus_tag="VF097_08905"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX6262950.1"
                     /translation="MARWATFDCYGTLIDWDRGIGDALAGLWPPADRQRLLAVFHAVE
                     PLIQDAEPALPYREVLDRAREAVGSILGLPAGEENVLAESLPTWPPFPEVPGVLRDLR
                     DRGWRLAILSNTDPDLLAASIARIAVPFDLTITAADAGSYKPSPGHWERFDALSGDRE
                     AQVHVGASAFHDIEPCARLGIPAIWVNRLGERSDLLRAAELPTLEGLPEALEDVA"
     gene            complement(2236..2736)
                     /locus_tag="VF097_08910"
     CDS             complement(2236..2736)
                     /locus_tag="VF097_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262951.1"
                     /translation="MRRAIVILALLAAACSGEAPADPAPEAEGGGVPYPYPFTTPTPP
                     REKTPLDGVWAREVPDELAGPVGKCRRCPPYRLELGDTNTLTIDRGTFEIEHTLHGWT
                     NAGHVFVEGDTATLINDPNCTSAEGVYRWTIDGDELTFELVEDGCAFGDLRPRYFTST
                     SWTRAG"
     gene            2814..3518
                     /locus_tag="VF097_08915"
     CDS             2814..3518
                     /locus_tag="VF097_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX6262952.1"
                     /translation="MGTVITRVFRGTVRPGQHAEFERLFREEAVPRFETTPGCLSVHF
                     GTPTEASPDEYLVVTVWENVESLKAFAGERWSDPKLSLREAHVLSDATVHHYRNGDGE
                     AIVPPASAHGGGPPAEVLDLGRLRVDLARRVAELDGGEVELPPQEFSVLAELALRAGE
                     PVPSDELARLVWPEDAWPTGEDVRRSVYRLRRLLGDQGRARPLIRTRRGHGYVLDPGE
                     RTSPRHRRHSRTAARA"
     gene            3531..3896
                     /locus_tag="VF097_08920"
     CDS             3531..3896
                     /locus_tag="VF097_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262953.1"
                     /translation="MRATALAAVLLLASCARLPDPGTLPGRYESTTAPATLALRDGQW
                     SLDNGGFVKSGAYGISGDRVAFLITETSHPAFDHYCREEADVYEWRFDDDLLVLRAVG
                     DTCDPVGQSVLTHGPWERA"
     gene            3893..4174
                     /locus_tag="VF097_08925"
     CDS             3893..4174
                     /locus_tag="VF097_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262954.1"
                     /translation="MSWEVRAARVMAWVTLVAGVGLAIACFVLAPVKDTTTERIVGHA
                     TITYEGVELVVRESFILAGIASLVLGAALWALLLASARAGERGRADTRS"
     gene            complement(4235..>5167)
                     /locus_tag="VF097_08930"
     CDS             complement(4235..>5167)
                     /locus_tag="VF097_08930"
                     /inference="COORDINATES: protein motif:HMM:NF014428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX6262955.1"
                     /translation="TDHTVTATVVDQFDDPVQGVVVLFDVSAEGTPSQTSGSGVTNAS
                     GQVSFSFTNSTAGVTNTITACMDTDPQNAGCDEGEETATATKAWIGVPVTTCPGFEGD
                     PRNQVVGTPGDDVLEGTEGDDIICGLGGNDTIAGLGGNDLILGGAGHDTANGGSGLDE
                     LIGGGGRDTLVGGADDDILRGAGGRDTLRGAAGSDLLVGAGGRDTLIGGPGSDTLRGG
                     ARNDDLRGGGGNDQLNGGGGRDTLRGAGGNDVLRGGGGNDDLRGGNGNDRMFGGAGDD
                     ALRGAGGNDLLNGGPGSDFCNGGQGADTLVSCES"
BASE COUNT          738 a         1840 c         1809 g          780 t
ORIGIN      
        1 gcacggtgac cgagaccggg accgggggcg ccgattcggt gacctactcc ccggacggcg
       61 gcaccccttc tgaccccccg accgtcgaga tcaccagcga cgggcaggag gtcacggtga
      121 cggtgaccaa caccttcatc gaggtcctgg gcgacatcac ggaccgcgac acggacaccg
      181 acaccgacgt cacgaccacg gaggagggcg gcacgctcgc attcaccgga gcggagctgc
      241 tgatcctcgc gctgatggca ctggggctcc tcgtgaccgg ggccccgctg ctcctcctca
      301 cgaggcgccg ggacgagtag cccgacacgc tccaagagga gcgggagcgc ccccgaggcc
      361 gtaggcctcg ggggcgctcc cgcgtccacc tctcagccca ggaagaccac ggtcacccag
      421 acaagtccat cgtcccggac cagccccacg cccaccctcc ggaaggggcg gtagaggatg
      481 ttccgacggt gcgcgtcgct gttcatgaac gcgccgtgga gcaagcggag cgtctcccgt
      541 agatccccgg tgggtccgac gacgccgacg ttctcgccca gccgctgcca ccgacggccc
      601 tggacctgat cgacgaggtt cgggttgtgg tgaagcgttc ccgtccttgc catccggcgg
      661 ctgtgccgtc gcgcgatcct ggagagcccg tggtcgagcc tgaacgtggc acgggccttg
      721 ggtcggcgct cttgattcat cagcctgcgt tcgacgcgct gagccttcgg ggccgcctcc
      781 gcctctccgt gcgccaccag ggagagaagg agcgtggcca ggagcatgag gatcgggatc
      841 ctcttgatcc ttgggtgcgc gagcgaaagc atcggtggac acctcctcgc ggtcccgcca
      901 ggacccgcga ggggtgtcct ggtccttcgg caggtcggct acccattggc gggggcccgt
      961 acctttgcgt cccgtcgtca ccgacggttt gcctttgtcg agaccgttcg tcgtcttgtg
     1021 ttgatcgtgc tcgcttccat gtatcggagg atggggcgga atgcttgagc gggctcagcg
     1081 cgagaggatc acgacgcgga cctcgaccag gccctcctcg agcggcgcca gccgggcgaa
     1141 agccgccctg gagagatcga tgatccgccc tgggacgaag gggccccggt cggtgaccgt
     1201 cacggtgacg ccgcggccgc cgtgctcaac gcggagccgc gtgccgcagg gaaggtggcg
     1261 gtgggcggtc gtgagcgcgt cgggatcata gggatcaccg caggcgctgg tgcgcccgcg
     1321 gaagggctcc ccgtaccagg aggcgacccc gaagagctcg gggcgctggg gacccggcgc
     1381 cgcctcgcgg agaggctcgt cccgcgaggc cggccccgga taggtgaagc ccgtgatcgc
     1441 ggcgagcgcg atcaccaaga cgaggacgcc gagcagcgcc cgttcagcat cccgggcctc
     1501 gcggggcatg catccatcct tccccgccct cccgtggatg actccggacc gagctcaggc
     1561 gacatcctcg agagcctccg gcaggccctc gagggtaggg agctccgcgg cccggagcag
     1621 atcgctccgc tccccgagcc gattgaccca gatcgctggg atccccagcc tcgcgcacgg
     1681 ctcgatgtcg tggaaggcgc tcgccccgac gtggacctgc gcctcgcggt cgccggacag
     1741 tgcgtcgaag cgctcccaat gccccgggga gggcttgtac gacccggcgt cggccgccgt
     1801 gatcgtcagg tcgaagggga cggcgatgcg cgcgatcgag gcggcgagca gatcgggatc
     1861 agtgttggag aggatggcca gacgccagcc ccggtcccgg aggtcgcgga ggacgcccgg
     1921 cacctcgggg aagggaggcc aggtcgggag cgactcggcc agcacgttct cctctccggc
     1981 cggcagcccg aggatcgagc cgacggcctc gcgggcccgg tccaggacct cccggtacgg
     2041 aagcgcgggc tcggcgtcct ggatcagcgg ctccaccgca tggaagacgg ccaggagccg
     2101 ctggcggtcg gccgggggcc agaggccggc cagcgcatcg ccgatgcccc ggtcccagtc
     2161 gatcagggtc ccgtagcagt cgaaggtcgc ccagcgcgcc acggggccaa ccttacgggt
     2221 gcgccttcgg cgacctcaac ccgcccgggt ccaggacgtc gaggtgaagt accgcggcct
     2281 gaggtcgccg aaggcgcacc cgtcctccac gagctcgaag gtgagctcat ctccgtcgat
     2341 cgtccagcga tacacacctt cggcggaggt gcagttcgga tcgttgatca gcgtggcggt
     2401 atcgccctcc acgaagacgt gtcccgcgtt cgtccacccg tgcagcgtgt gctcgatctc
     2461 gaacgtccct cgatcgatcg tcagagtgtt cgtgtccccg agctcgagcc ggtacggggg
     2521 gcagcgccgg cacttcccca ccggtcctgc cagctcgtcc gggacctccc tggcccagac
     2581 gccgtccagg ggcgtcttct cgcgtggggg ggtcggcgtg gtgaagggat aggggtacgg
     2641 aacgccgccg ccctccgcct cgggggcggg gtcggcaggg gcctcaccgg agcatgcggc
     2701 ggcgagcagg gcgaggatca ctatcgctcg tcgcatggcg atagggaacc acggtagcgg
     2761 cgagcgcacc atggcctcga ggtcgccgtc acgtcctcgg ctcatggagg acaatgggga
     2821 ccgtgatcac gcgggttttt cgggggacgg tgcggcctgg ccagcacgcg gagttcgagc
     2881 ggctgttccg cgaggaggct gtgccgcgct tcgagacgac ccccgggtgc ctctccgtgc
     2941 acttcggaac gccgacggag gcttcccccg acgaatacct ggtcgtgacc gtctgggaga
     3001 acgtcgagtc gctcaaggcc ttcgccggcg agcgctggtc ggacccgaag ctcagtctcc
     3061 gggaggccca cgtgctgtcg gacgcgaccg tccatcatta ccgcaacggc gatggggagg
     3121 cgatcgtgcc gccggcctcc gcccacggcg ggggtccgcc cgccgaggtg ctcgacctgg
     3181 gacgtctccg cgtcgacctc gcccgccgcg tggccgagct ggacggcgga gaggtcgagc
     3241 ttcctcccca ggagttctcg gtgctggccg agctggcgct tcgggcggga gagcccgtcc
     3301 cctccgacga gctggcccgc ctcgtgtggc ccgaggacgc ctggcccacc ggtgaggatg
     3361 tccgccgctc cgtgtatcgg ctgcgcaggc tcctcggcga tcagggacgg gcccggcccc
     3421 tcatccggac ccgccggggg cacggctacg tcctggatcc gggcgagagg acatcgccgc
     3481 gacaccgccg ccattcacgc accgccgccc gtgcctgaga ctgcccaagg atgagagcca
     3541 ccgccctggc agccgtcctg ctcctcgcct cgtgcgcgag gctacccgac cccgggaccc
     3601 tgccgggcag gtacgagagc accaccgcgc cggccacctt ggccctgagg gacggccagt
     3661 ggagcctgga caacggcggg ttcgtgaagt cgggcgcgta cgggatctcc ggcgaccgcg
     3721 tggccttcct gatcacggag acgagtcacc ccgcgttcga tcactactgc cgcgaggagg
     3781 ccgacgtgta cgaatggcgc ttcgacgatg atctgttggt gctccgggcg gtcggggata
     3841 cgtgcgaccc cgtggggcag tcggtcctca cgcacggacc gtgggagcga gcatgagctg
     3901 ggaggtgcgc gctgctcgag tgatggcctg ggtgacgctc gtcgccggcg tcgggctggc
     3961 gatcgcttgc ttcgtcctgg cgccggtgaa ggacacgacg accgagcgca tcgttgggca
     4021 tgccaccatc acgtacgagg gcgtcgagct cgtcgttcgt gagtccttca tcctcgcagg
     4081 gatcgcctcc ctcgtgctgg gcgctgcgct ctgggcgctc ctgctcgcct ccgcccgagc
     4141 tggggagcga ggaagagccg atacccggtc atagggaatg atgcgcgggc cggcccggag
     4201 gccggcccgc gccgttcacc tgcggacggc tcgcctagct ctcgcagctg acgagcgtat
     4261 cggccccctg acctccgttg cagaagtcgg agcccggacc gccgttcagc aggtcgttcc
     4321 caccggctcc gcgcagggca tcgtcgccag cgcccccgaa catccggtcg ttgccgttgc
     4381 ctcctcggag atcgtcgttc cctccgcctc cgcgaaggac gtcgttcccg ccggcaccgc
     4441 gcaacgtgtc gcgcccgccg ccgccgttga gttgatcgtt cccgccgcct cctcggaggt
     4501 catcgttgcg ggctccaccc cgcagcgtgt cgctgcccgg gccaccgatc agggtgtcgc
     4561 ggccacccgc gcccacgagt aggtcggagc ccgcggcgcc gcgcaacgtg tcgcggcccc
     4621 cagcgccgcg gaggatgtcg tcgtccgccc cgccaacgag cgtgtcgcgg ccaccgccgc
     4681 cgatcagctc atccaagccg gagccgccat tggcggtgtc gtgccccgct cccccgagga
     4741 taaggtcgtt gcctcccagg cccgcgatcg tgtcgttgcc accgaggccg cagatgatgt
     4801 cgtcgccctc cgtgccctcg agcacgtcgt cgcccggggt gccgacgacc tggtttcgag
     4861 gatcaccctc gaacccgggg cacgtggtca ccggaacacc gatccacgcc ttcgtggcgg
     4921 tggccgtctc ctcgccctcg tcgcaccccg cgttctgggg atcggtatcc atgcaggcgg
     4981 tgatggtgtt cgtcacgccc gcggtcgagt tcgtgaagct aaacgacacc tgcccgctcg
     5041 cgttcgtgac ccccgaaccc gacgtctgcg acggcgtccc ctccgcactg acgtcgaaca
     5101 ggacgaccac gccctgcacc ggatcgtcga actgatcaac caccgtcgcc gtcactgtgt
     5161 ggtcggt
//
LOCUS       DASUTZ010000205        19652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1754538, whole genome shotgun sequence.
ACCESSION   DASUTZ010000205 DASUTZ010000000
VERSION     DASUTZ010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 19652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19652
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1754538"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1191)
                     /gene="rodA"
                     /locus_tag="VF097_08935"
     CDS             complement(49..1191)
                     /gene="rodA"
                     /locus_tag="VF097_08935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX6262956.1"
                     /translation="MALEAAPARRWLGDVAPIRHLDPTLIFVSIGLTIYGLLMVYSAT
                     HRTLATFGEDPGYYLKRQLAFMMLGLIVLVVASVVDYRLVKVYAPFIFAICGFLLFLV
                     LTPLGSETAGAQRWVSLFGFQFQPSEVTKLALIAMLAAYLSEIRGPVRLEHVWRATGL
                     TVIPIALVFIQPDIGTSMVFAVILVTMLIVSGAKAKQIGILAIAALIALFAAFRLGVV
                     QDYQIERLTGFLDPANDPLRAGYNRAQAEIAIGAGGLFGTGYGSGTQTNLDFVPEQHT
                     DFIFTVVGEEFGFVGAVLLLSMYGILVWRGFRIAMLSKDAFGSLLALGIVGMLAFQVF
                     VNVGMTLGIMPITGIPLPFLSYGGTALLTNFLAVGLLLNVHMRRFK"
     gene            complement(1191..3095)
                     /gene="mrdA"
                     /locus_tag="VF097_08940"
     CDS             complement(1191..3095)
                     /gene="mrdA"
                     /locus_tag="VF097_08940"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX6262957.1"
                     /translation="MSEDRMSNRMRVLAAFVGFLFAALVTRLWFLQVLAAEEYRERAE
                     SNRVRIIPEPAPRGRILDAHGRVLVDNRRSIQVIVNRRHVENPEELLFRLSEVLEVPA
                     EELAERYEDPTYLPYQPVPVADDVPERKAAYLVAHRREFPGVSYRVVGVRDYVHGSLA
                     AHVLGYLGEINEEELKSPAFRGYRPGDEVGRGGVEQHYEEFLRGREGYRSIQVDAQGR
                     SGQVLGSRRPEPGSDLYLSIDLGLQTAVEESLAQGVRAANGVVTSEGRTLNAKAGAVV
                     ALDPNTGHVRAMASLPAYNPDVFVDGLTQREWRRFDRDPTHPLQNRAIQGLYPAASTF
                     KPFVGAAAVKAGIADPESYFPCPPTFMVPGDTSETVFHNWHSAHEGSLSLAGSLERSC
                     DTIYYQFGLDFWRLRDDRGDVMQRQLRKWGFGRPTGVDIPGELEGRVPDSEWKRQVHE
                     LYPQYFPEGIWLPGDNINMSIGQGDLLVTPLQLASAYAAIANGGALYRPEVGLRVEAL
                     DGTVLKAVAPREIRTIPMQKKPREQILAGLRAAVQGGGGTATTAFAGFPESLAGKTGT
                     AEVIVDGQETTHSWFAAFAPAEDPEIVVVAIVEEGGHGSQVAAPIVRRVLEQYFGITP
                     SAFRIGVATD"
     gene            complement(3097..3603)
                     /gene="mreD"
                     /locus_tag="VF097_08945"
     CDS             complement(3097..3603)
                     /gene="mreD"
                     /locus_tag="VF097_08945"
                     /inference="COORDINATES: protein motif:HMM:NF016019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX6262958.1"
                     /translation="MRRGLAWAVVVVTALLLQSTVFAQVKLAGAKPELMFLITIVMAM
                     LEGPSVGAITGFTAGMGQDFLLDQPKGITALTLTLVGYAVGQVRQYIVTPGPLTPVLL
                     VGGFTAIGVLFYGLVAFLLGQLGTGFLFLLRTALLSAVYNAVLTPLLYPVLKRVAEAS
                     KTSRVHRW"
     gene            complement(3642..4487)
                     /gene="mreC"
                     /locus_tag="VF097_08950"
     CDS             complement(3642..4487)
                     /gene="mreC"
                     /locus_tag="VF097_08950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX6262959.1"
                     /translation="MAIYRRERSTRLLVVALVVTSLVIITLDFRGGQRGPLAAAGRIS
                     LAIISPLQDGIAKVFRPIGSFFSNLASLGSLRAENERLQAELAELQAVRVRFLEYERR
                     IKQLTALLEFRETQEVETIGATVISEVPSNFEASVTIDRGSADGVELDMPVVAAEGLV
                     GRVVSVSKRWSKVLLLIDVDHAVHARLASTGETGILRGQRERDLRFDLIDPGTPVEAG
                     EPVVTSGQDGVYPPGIEIGSVSHVAPNESALQEQILVRPYVDVSRLSDVLVVLSGGVV
                     EGSQR"
     gene            complement(4515..5537)
                     /locus_tag="VF097_08955"
     CDS             complement(4515..5537)
                     /locus_tag="VF097_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX6262960.1"
                     /translation="MTGLLGRDMAVDLGTANTLVYVRGRGIVLNEPSVVAINTMTKQV
                     LAVGAEAKRMIGRTPSHIQAVRPLKDGVIADFDITEKMLRYFIQKVHTRRFLAKPRVV
                     VCVPSGITGVEQRAVEEATISAGARAAYIIEEPMAAAIGAGLPVHEPTGNMVVDIGGG
                     TTEVAIISLGGIVTATSVRVGGDELDEAIIQYVKKEYSLVLGERTAEAIKMAIGSAFP
                     MPDEMIAEIKGRDLVSGLPKTIHVSAEEIRKGLEEPVNAVVDAIKNTLDRCPPELAAD
                     IMDQGIVLTGGGALLKGLDERLKHETGMPIHVAENPLACVVMGSGKCLEEFEALKQVL
                     ASSTRR"
     gene            5919..6842
                     /locus_tag="VF097_08960"
     CDS             5919..6842
                     /locus_tag="VF097_08960"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX6262961.1"
                     /translation="MRIAVLFPGQGSQYAGMAEPWTDHPRGKETLTEASDVLGYDVVA
                     VAGDEEKLGLTDVVQPAMFACDLAAFRVLEAEGATFAMTAGHSLGEFVALAAAGAVPV
                     DQALTAVVERGRAMQEASDASPGGMTALVGVSPQEAAEIVATAGRGDVLTVANENSPK
                     QTVLSGSISAIERAEELARSRKVRTVRLKVAGAFHSKLMEPALARVRRAVAKLDFSEP
                     RFPVVPNASGRPTSQPSALRDLLSRHLVSPVKWERSIRAMADAGIELFVEAGPGDVLS
                     KLVKRCVPGSRAVPVGSPAEARAFLDEEGVR"
     gene            6839..7123
                     /locus_tag="VF097_08965"
     CDS             6839..7123
                     /locus_tag="VF097_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262962.1"
                     /translation="MTVTFEPYGHTPTTLRVLVSPGAGRLRYCPPRRFAGGRELVEAG
                     QPVARVQQGSEEVILRAPVGGVVSSVVGIEGEPLVPGQAILAIEPATEGS"
     gene            7120..8124
                     /locus_tag="VF097_08970"
     CDS             7120..8124
                     /locus_tag="VF097_08970"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX6262963.1"
                     /translation="MTGRHAQIAGLGSALPERVVTNDDLAAMGVDTSDEWIRARTGIG
                     ARRFVAEGETASMLGAQAARKALEAAGVDPGAVDLTISATFTGDQPLPSTASFLQAEL
                     GIYGAAFDLAAACAGFIYATEMARGQIVTGGAETVLVVGVEVLSPILNLEDRTTCVLF
                     GDGAGAAVLVPGDEPGIIGSVLENDGRHAHMLEVPSGGSASPTSSETLARGEHYVRMA
                     DGREVFKRAVRGMSDAARRLLEKAGLALDDVTMLVPHQANARIIRAVAERLSVPPERV
                     YIDLEEVGNTSAASVPIALDHAWREGRLSPGDLVLTAAFGAGLAWGANLIRWTAEPPA
                     "
     gene            8121..8858
                     /locus_tag="VF097_08975"
     CDS             8121..8858
                     /locus_tag="VF097_08975"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEX6262964.1"
                     /translation="MSGRIALVTGAGGGLGRAAALELAGRGMAVACAYATNDAGAKET
                     AELIEERGGAAQAFGADVADEAQVRNLFAEVKEWRGAPLVLVANAGVRRDGPTVKYPL
                     EQWQRTLDVNLTGVFLCVREALRGMQLARWGRVILVSSAAGLRGNPGQASYSASKAGV
                     LGLTRSLSREVGARGITVNAVAPGYVETAIVAEMTEAARERMMGLIATGRPGRPEEIA
                     AAIGFLASEEASYVTGAVLPVDGGMTA"
     gene            8890..9141
                     /gene="acpP"
                     /locus_tag="VF097_08980"
     CDS             8890..9141
                     /gene="acpP"
                     /locus_tag="VF097_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009556086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX6262965.1"
                     /translation="MERKEIEERVRKVLAEQLARDPDEVTLEASFEDDLDADSLDLVE
                     AVLALEEEFGIEIPEEEMEGVKTVKEAVDLAASKLGVSA"
     gene            9142..10371
                     /gene="fabF"
                     /locus_tag="VF097_08985"
     CDS             9142..10371
                     /gene="fabF"
                     /locus_tag="VF097_08985"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX6262966.1"
                     /translation="MTERKVVVTGIGPVTPVGTGREAFWDALASGRSGIGEITLFDAS
                     EHPVRIGGEVEGFDAADWMDRKAAKRTDRAVHLAVAAAKLAWEDAGRPQIDPERAAVV
                     VSTGVGGLRYMEEQIKIMNDRGPGRVSAFTVPAMMANAATGTVAMEFGFTGPNACITT
                     ACAAGAHGVGEGYRYIKHGMADLALAGGTEAGITPLSLAAFAQMQALSRNADPERASR
                     PFDADRDGFVISEAACVLVLEEEERARTRGATIYAEVAGYGTSADAFHITQPEPQGSG
                     AVIAMQMALTDAGEPAEAVDYINAHGTSTPLNDAAETRAIKKAFGDYAYEVAVSSTKS
                     MTGHMVGATGAAEAAVAALAVRDGVIPPTVNYETPDPECDLDYVPNEKRETDVRLALS
                     NAFGFGGQNAVLALRRI"
     gene            complement(10372..11112)
                     /locus_tag="VF097_08990"
     CDS             complement(10372..11112)
                     /locus_tag="VF097_08990"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEX6262967.1"
                     /translation="MAISVAPKARVGARLKEARARRGVSVEDAALHTRIKPRFLRALE
                     ADAPPSAFRAPVYARAFLREYAGFLGLDPESLVADYASVHGIGDGQSIRLPRPVERRS
                     HKKLIATLLWIASLGAMGALVVVSARSVERSIPPVTLPAETSPPAAEAAAPAGGENEI
                     VLRLRVKDARTWIRVRVDGELAEDGRRKPGFVKTFRADRRIRLELANAGAVRLRLGGE
                     PLGAPGAEGVTYRATFVLVDGEVQVRRP"
     gene            complement(11188..11592)
                     /gene="ndk"
                     /locus_tag="VF097_08995"
     CDS             complement(11188..11592)
                     /gene="ndk"
                     /locus_tag="VF097_08995"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX6262968.1"
                     /translation="MTTQETLVIAKPDAVRRRLVGEILRRIEDRGLRITGLRMMRIDQ
                     DLADRHYEEHRDKPFFGELVEFITSGPVVVARIEGEDAVAAVRELMGPTDPKDAPSGT
                     IRGDYGEVITENLVHGSDSPESAKRELGLFFG"
     gene            complement(11594..12874)
                     /locus_tag="VF097_09000"
     CDS             complement(11594..12874)
                     /locus_tag="VF097_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX6262969.1"
                     /translation="MRFEDAVADLEARGEGRMIPTLDRIRAVVQLMGDPQLAYPTVHV
                     AGTNGKTTTTRLVERLLCAHGVSAGALTSPHLHTVRERLTLCGDPIGEGEFAETYAHL
                     RLFLEEVDREGERITYFETLAALAALWFADKPVDVGVFEVGMGGSWDATNVIRSEVAV
                     ITPIGLDHPELGGTVEEVAAEKAGVLKEGTRAVVREQRPEARKVLEARAAEVGAILLH
                     EGDGFTVPVRDLAVGGQRLAVRGLRGEYRGLSLPLHGEHAARNAAAAVAAVELTLDRE
                     LGADAVREALAGASSPGRLEVVARHPLVVLDGAHNPDGAAALAAALGEAFIWEHLYLV
                     LAVLETKDAAGIASVLASRTTSAFVAASSHPRAMDADRLTAVAERAGIPAMPHPTVAA
                     ALDAATKAAGPDDLILVTGSLYTVADVRPRYVKG"
     gene            complement(12876..15515)
                     /locus_tag="VF097_09005"
     CDS             complement(12876..15515)
                     /locus_tag="VF097_09005"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX6262970.1"
                     /translation="MTMASAYEPSEVESRLYAEWIERGYFRAEPTDEREPFCIVLPPP
                     NVTGALHIGHALDHTIQDALIRRRRMQGYEALWLPGTDHAGIGTEVLVGRRLLEEGID
                     KQAIGREAFIERVWEWRERYGGRIVEQMKALGSSCDWDRLRFTMDEGLQHAVRVAFVR
                     LYEDGLIYRGERIINWCPTDQTALSDSEVEHEEVEGELITFRYPMADGEGHIEVATTR
                     IETMLGDTGIAVHPDDERYRDLIGRSVRHPVDGRELPVVADEAVDPSFGTGAVKVTPA
                     HDPNDFEIAERTGLEARNILTSEARLNDVVPEAFRGLDRYEARRAVRDRIDEMGLLVR
                     EERPYVHSVGHCYRCRTEIEPWLSGEQWFVKVDPLKGPAMEAAESGRLAFSPPRWKDR
                     YLEWLEGLRDWNISRQLWWGHRIPVWYCSNGHEFASIEDPEACAECGSAEIEQDPDVL
                     DTWFSSQLWPFSTLGWPEETEDLRYFYPTSVLVTGYEILYLWVARMVMSGLRLTGDLP
                     FHTVAIHGLVRDAQGRKMSKSLGNVIDPLDMIERYGADALRFALSRMASPEQQNLPLS
                     EDQIEAGKHFANKLWNAARLVRANLDGGDLPPPSPERLTVTDRWLLSRAEACRAEVDA
                     AMETYRFADAAQALYRFLWSEYCDWGLEMAKPRFYEGSEEGRRDAATVLAWVLERSLR
                     LLHPIMPFVTEEIRRRFVPDGSIMVAPWPEPMPAHRDEEAEGRVGFAMDLVTAVRRFR
                     SDHRVPPSIELDVRVEGDGAQREILGTLSEEVRRLARVGSIVMTGEASSPSASARLHV
                     QGAEVFIPLEGVLDPEAECDRLRGRIEALGEEAGKAERKLANEGFVTKAPAEVVKEQR
                     RRLSRLKEEEAALREQLDELGCD"
     gene            15638..18466
                     /locus_tag="VF097_09010"
     CDS             15638..18466
                     /locus_tag="VF097_09010"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX6262971.1"
                     /translation="MVQREGPGPGGAAPGAPAPPAVAGGLIVRVFQETPDVILMLQVS
                     ADGYLRCQTAISSFPTAPSLAALQGLTIEQILPPEILSRFGRRCQEATERKGPIRYQE
                     HLRVGDSDVVLDAAITPIFDGEGASTHLFWSARNVTDEWTTRESLRRTEERLRTLERE
                     HAKALASRSEAGEAAIERRGKELEERTQKAEELGRQLEERATEIESRERAMEEQREAL
                     EGQARLIEQREKTLEERIGTLDAERRSMLEKAGTHDEQRKALEDLRSTLEEERASIDA
                     HRAKLEHDRSELDEAKAELEKRRANVEAERDALRARVEELERSAGEQEERAAEVEAQR
                     ELVETERETLQALKASLSEERASLEAEKEGLEAEKEALEARLAQVESRRETAAGHADH
                     LFQVSTTGVAWSDGDGKLREVNRALAAMMRRSPEDLTGISLQDLLHADEQDAIERLLA
                     EARESEGGVAQTELRFAHEDGESSHVHVTLTAVREGDDGDDRFLWQVVDYTERRGLQE
                     RLAHQALHDSLTGLPNRALFLDRLSRALQRLDRNPGTVAVMIMDLDHFAQVNEQNGHE
                     VGDRVLAHVAERLGESIRGSDTVGRLESDTFGILCEELSDAEDAQGVADRLRRAVEGR
                     AMIDELGVDVGVSIGVALTDETTDRPETLLRDATLAVREAKEAGRARTEIFDRDRREE
                     AAQRFRTESDLARAIEEDEFRVFYQPQIDLQGGTIVGVEVLVRWQQPDSGLVTPSEFI
                     PLAEQTGLIVPLGAWILEQACLQSKRWASVAGTELATWVNMSPRQLAQPDLPDMTEKV
                     LAQTGADPATLCVEVTEGVLLEDTGAAVGNLRALKELGLRVGIDDFGKGFSSLAYLKR
                     LPVDMLKIDRTFVQGLGQSKEDTAIAEAVIGLARAMDLTSIAEGVETPEQLARLREMG
                     CDLAQGYLFASPQPAEVIEQLLTQDLRW"
     gene            complement(18473..>19652)
                     /gene="clpX"
                     /locus_tag="VF097_09015"
     CDS             complement(18473..>19652)
                     /gene="clpX"
                     /locus_tag="VF097_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX6262972.1"
                     /translation="GVYICDECIDLCNEIIEEELSETSEIKLEELPKPREIYEFLNDY
                     VIGQDQAKKVLSVAVYNHYKRVQVEGGGGADDVELQKSNILLLGPSGCGKTLLAQTLA
                     RMLNVPFAIADATALTEAGYVGEDVENILLKLIQAADYDVKRAETGIIYIDEIDKIAR
                     KAENPSITRDVSGEGVQQALLKILEGTTASVPPQGGRKHPHQEFIQIDTTKILFICGG
                     SFAGLEKIIESRIGRKGIGFGADVRSSEEKDVGESLALGLPEDLLKYGLIPEFVGRLP
                     VITHVHQLDKDALIDILTKPKNALVRQYHKFFEFDGVELDFTPGALEAIAEQSIRRQT
                     GARGLRAIMEEVLLNVMYDLPGRSDVDRCVIDEQVVMEGVNPTLVPMDQEPERKERSA
                     "
BASE COUNT         3020 a         6897 c         6912 g         2823 t
ORIGIN      
        1 acatgcacca ccaggatagc aggaggaggt gccggctccg ggaggctcct acttgaacct
       61 gcgcatgtgc acgttcagca ggaggcccac cgccaggaag ttggtgagca gcgcggtgcc
      121 cccgtaggag aggaacggta aggggatccc cgtgatcggc atgattccca gggtcatccc
      181 cacgttgacg aagacctgga acgcgagcat ccccacgatc cccagggcga gcagcgagcc
      241 gaaggcgtcc ttcgacagca tggcgatcct gaacccgcgc cagaccagga tgccgtacat
      301 cgaaaggaga aggacggccc ccacgaaccc gaactcctca ccgaccaccg tgaagatgaa
      361 gtcggtgtgc tgctcgggca cgaagtcgag gttcgtctgg gtccccgacc cgtaccccgt
      421 cccgaacagg ccgccggcgc cgatcgcgat ctcggcctgc gctcggttgt agccggcgcg
      481 aagcggatcg ttcgcggggt cgaggaagcc cgtcagccgc tcgatctggt aatcctgcac
      541 cacccccaga cggaacgcgg cgaagagcgc gatcaaggcc gcgatcgcca ggatcccgat
      601 ctgcttcgcc ttcgcccccg acacgatgag catcgttacg aggatcacgg caaagaccat
      661 cgaggtcccg atgtcgggct ggatgaacac cagggcaatc gggatcaccg tcaggccggt
      721 ggcccgccag acgtgctcga ggcgcacggg gccccggatc tccgagaggt aggccgccag
      781 catcgcgatg agcgccagct tcgtcacctc cgagggctgg aactggaagc cgaacaggct
      841 cacccagcgc tgggcgccgg ccgtctcgct gccgagcggg gtcagcacga ggaagaggag
      901 gaacccgcag atcgcgaaga tgaacggcgc gtacaccttc accagccggt aatcgacgac
      961 cgaggcgacc acgagcacga tgagccccag catcatgaag gcgagctgcc gcttgaggta
     1021 gtagcccgga tcctcgccga acgtggccag cgtccggtgg gtggcggagt agaccatcag
     1081 caggccgtag atcgtcagcc cgatgctgac gaagatcagc gtgggatcga ggtggcggat
     1141 cggggccaca tcgccgagcc atcgccgcgc cggagccgcc tcgagggcca tcagtccgtc
     1201 gccaccccga tgcggaacgc gctgggcgtg atcccgaagt actgctcgag gacccggcgc
     1261 acgatcggcg ccgccacctg cgagccgtgg ccgccctcct cgacgatggc aaccaccacg
     1321 atctccggat cctctgccgg ggcgaacgcg gcgaaccacg agtgggtcgt ctcttgaccg
     1381 tccacgatca cctcggcggt gccggtcttg ccggccaggg actccgggaa gccggcgaac
     1441 gccgtggtcg ccgttcctcc gccgccctgc acggccgccc gcagaccggc caggatctgc
     1501 tccctcggct tcttctgcat ggggatcgtt cggatctcgc gcggcgccac ggccttgagg
     1561 accgtcccgt cgagcgcctc aacgcgaagg ccgacctctg gccggtacag cgcgcctcca
     1621 ttcgcgatcg ccgcgtaggc ggaggcaagc tgcagcgggg tcacgaggag atccccctgc
     1681 ccgatcgaca tgttgatgtt gtcgccgggg agccagatgc cctccgggaa gtactgcggg
     1741 tagagctcgt gcacctgccg cttccattcg ctgtcgggga cccgaccctc cagctcgccc
     1801 gggatgtcga ccccggttgg ccggccgaac ccccacttcc ggagctgtcg ctgcatcacg
     1861 tcgccgcgat cgtctcgcag ccgccagaag tccagcccga actggtagta gatggtgtcg
     1921 cacgatcgct ccagcgagcc agccagcgag agggacccct catgggccga gtgccaattg
     1981 tggaacaccg tctccgacgt gtcccccggc accatgaacg tcggggggca cgggaagtag
     2041 ctctcggggt cggcgatgcc ggccttcacc gcggccgccc ccacgaaggg cttgaacgtg
     2101 gaggccgcgg gatagaggcc ctggatcgcc cggttctgca gcggatgggt cggatcacgg
     2161 tcgaaccgcc tccattccct ctgggtgagg ccgtccacga acacgtcggg gttgtaggcg
     2221 gggagggagg ccatggcacg gacgtgaccg gtgttcgggt ccagcgcgac cacggccccc
     2281 gccttggcgt tgagcgttcg gccctccgag gtgacgaccc cattggcggc ccggacgccc
     2341 tgggccaggc tctcctccac ggccgtctgc agccccaggt cgatcgatag gtacaggtcg
     2401 ctgccgggct ccggccgacg ggagccgagc acctgcccgg accgaccctg cgcgtcgacc
     2461 tggatgctcc ggtagccctc cctgcctcgg aggaactcct cgtagtgctg ctcaacgccg
     2521 cctcggccga cctcgtcgcc cggccggtag ccccggaacg cgggtgactt cagctcctct
     2581 tcgttgatct ctcccagata gccgagcacg tgggccgcga gcgacccgtg cacgtagtcg
     2641 cgcacgccga ccacgcgata cgagacgccg gggaactccc tgcggtgcgc cacgaggtag
     2701 gccgccttcc gctcgggcac gtcgtcggcc acgggcaccg gctgatacgg caggtaggtg
     2761 gggtcctcgt agcgctccgc cagctcctcc gccggcacct cgagcacctc ggagaggcgg
     2821 aaaaggagct cttccgggtt ctcgacgtgg cgccgattga cgatcacctg gatgctccgc
     2881 cggttgtcca cgaggacccg cccgtgcgca tcgaggatcc tcccacgcgg ggccggctcc
     2941 gggatgatcc ggacgcggtt ggactcggct cgctcccggt actcctcggc ggcgaggacc
     3001 tggaggaacc acagccgggt caccagggcg gcgaacagga acccgacgaa cgccgcgagc
     3061 actcgcatcc ggttcgacat ccggtcctcg ctcatgtcac cacctgtgca cccggctcgt
     3121 cttggacgcc tcggccacgc gcttcagcac ggggtacagg aggggcgtca gcacggcgtt
     3181 gtagaccgcg gacagcaggg ccgtccgcag caggaagagg aagccggtcc cgagctggcc
     3241 cagcaggaag gccacgagcc cgtagaagag cacgccgatc gccgtgaacc cgcccaccag
     3301 cagcacgggg gtgagagggc cgggcgtgac gatgtactga cgcacctgcc ccaccgcgta
     3361 gcccacgagc gtgagcgtca gggccgtgat gcccttgggc tgatcgagga ggaagtcctg
     3421 ccccatcccc gccgtgaacc ccgtgatggc gcccaccgac ggcccctcga gcatcgccat
     3481 cacgatcgtg atgaggaaca tcagctccgg cttcgctcct gcgagcttca cctgggcgaa
     3541 gacggtggat tgcagcagca gggccgtgac gaccacgacg gcccaagcga gtccgcgtcg
     3601 cacggagagt cagtctatcc ccctccatgt ccccggtccg cctaccgctg actgccctcg
     3661 accacgccgc cggagaggac gaccagcacg tccgacagcc gggacacgtc gacgtatggg
     3721 cgcacgagga tctgctcctg cagggccgac tcgttcggcg cgacgtgcga gacggacccg
     3781 atctcgatgc cgggcggata gacgccgtcc tgacccgagg tcacgacggg ctcacccgcc
     3841 tcgaccgggg tccccgggtc gatcagatcg aacctcaggt cccgctcccg ctgcccccgg
     3901 aggatccccg tctcgccggt ggaggcgagc cgcgcgtgga ccgcgtgatc cacgtcgatc
     3961 agcaggagga ccttggacca gcgcttggag accgacacca cccgtcccac gaggccctcg
     4021 gcggcgacca cgggcatgtc gagctccacc ccgtcggccg agccccggtc gatcgtcacg
     4081 gaggcctcga agttcgaggg aacctcgctg atcacggtgg ccccgatcgt ctcgacctcc
     4141 tgcgtctctc tgaactccag gagcgcggtg agctgcttga tccgccgctc gtactcgaga
     4201 aaccggaccc gcacggcctg gagctccgcc agctcggcct ggagccgctc gttctcggcc
     4261 cggagggagc ccagcgaggc gaggttgctg aagaagctcc cgatcggacg aaagaccttg
     4321 gcgatgccgt cctggagcgg gctgatgatg gcgagggaga tccttcccgc ggcggccagc
     4381 ggtccccgct ggccgccgcg gaagtccagc gtgatgatga cgaggctcgt cacgaccagc
     4441 gccaccacga gcagacgggt gctccgctcg cgcctgtaga tcgccatacg ctgggagtct
     4501 agccctgcgg gttgctagcg tcgcgtcgag gatgcgagga cctgcttcag tgcctcgaac
     4561 tcctccaggc acttcccgga ccccatcacc acgcacgcca gcgggttctc agccacgtgg
     4621 atcggcatcc cggtctcgtg cttcagccgc tcgtcgaggc ccttgaggag agctccgccg
     4681 cccgtcagga cgatcccctg gtccatgatg tcggcggcca gctcgggtgg gcagcggtcg
     4741 agcgtgttct tgatcgcgtc caccacggcg ttgaccggct cctcgaggcc cttgcggatc
     4801 tcctctgccg agacgtggat cgtcttgggc agcccgctga cgaggtcacg gcccttgatc
     4861 tcggcgatca tctcgtcggg catggggaag gccgacccga tcgccatctt gatcgcctca
     4921 gccgtccgct cgccgagcac gagcgagtac tccttcttca cgtactggat gatcgcctcg
     4981 tccagctcgt caccgcccac gcggacggag gtggcggtga cgatgccgcc cagcgagatg
     5041 atggcgacct cggtggtgcc gcccccgatg tccacgacca tgttgcccgt cggctcgtgg
     5101 accgggagcc cggcgccgat cgcggcggcc atcggctcct cgatgatgta cgcggcgcgg
     5161 gcgccggccg agatcgtcgc ctcctcgacc gcacgctgct ccaccccggt gatgccgctc
     5221 gggacgcaga ccacgacgcg gggcttggcc aggaaccgcc gcgtgtgcac cttctggatg
     5281 aagtaccgca gcatcttctc ggtgatgtcg aagtcggcga tcacgccgtc cttcagcggg
     5341 cggacggcct ggatgtggga cggggtccgg ccgatcatcc gcttcgcctc ggcccccacg
     5401 gccaggacct gcttcgtcat cgtgttgatc gcgaccacgg agggctcgtt caggacgatg
     5461 ccccgcccgc ggacgtagac cagggtgttg gcggtgccga ggtccacggc catgtcccga
     5521 ccgaggagcc cggtgatgcc ctccggccgg cggtcgcggg agagccgctc ggtccgctgt
     5581 ggctcaccgt tctgtgtcaa tctcaccttc ccggggaagc tcgaggccgg atggcctctg
     5641 tctacaggac tccataccgt aatgcaacga cgcggccgcg tccaaccggg tccgtttgtg
     5701 cgcaaccgtc gcgaagggta caacgaaacg gtgccaacgc gctggccttc gcggtgtggc
     5761 gacgggtggg gcggtcccgg cctccactca acctaccgag tagtatgtct gacctgcgga
     5821 aacctaccgg ccgttaagtt gcgcgagccg ggtaaccgtg gcaccctgcg tcgagtctga
     5881 gaagcgggtc gcccacccaa atctccgagg gaggtgtcat gaggatcgcc gtgctgttcc
     5941 cgggccaagg gtcccagtac gccgggatgg ccgagccctg gaccgaccac cccaggggca
     6001 aggagaccct caccgaagcc tccgacgtcc tggggtacga cgtcgtggcg gtggcgggcg
     6061 acgaggagaa gctggggctg accgacgtcg tccagccggc gatgttcgcc tgcgatctgg
     6121 ccgcgttccg ggtcctcgag gccgagggcg cgaccttcgc catgaccgcc ggacactccc
     6181 tcggcgagtt cgtggcgctg gcggcggcgg gcgccgtccc ggtcgaccag gcgctcaccg
     6241 cggtggtcga gcggggacgc gcgatgcagg aggcctccga cgcctccccc ggcgggatga
     6301 cggccctggt cggcgtctct ccccaggagg ccgccgagat cgtggccacc gccggacggg
     6361 gcgacgtcct gaccgtggcc aacgagaaca gccccaagca gaccgtcctc tcggggtcga
     6421 tctccgcgat cgagcgggcc gaggagctgg cccgcagccg caaggtccgc acggtgaggc
     6481 tgaaggtcgc gggggccttc cactcgaagc tgatggagcc cgccctcgcc cgcgtccggc
     6541 gggcggtcgc caagctcgat ttctcggagc cccggttccc cgtggtaccg aacgcgagcg
     6601 ggcgccccac ctcacagccg tccgccctcc gcgacctgct ctcccgtcat ctggtgtccc
     6661 cggtgaagtg ggagcgctcc atccgcgcga tggcggacgc cgggatcgag ctcttcgtgg
     6721 aggcgggccc cggcgacgtc ctgtccaagc tggtgaagcg ctgcgtcccc ggctctcgcg
     6781 ccgtcccggt gggaagcccc gcggaggcgc gagcgttcct cgacgaagaa ggagtccgat
     6841 gaccgtcacc ttcgagccct acggccacac gccgaccacg ctccgggtgc tcgtgagccc
     6901 cggcgcgggc cggctccgct actgtccccc gcgccgcttc gcgggcgggc gcgagctcgt
     6961 ggaggccggg cagcccgtgg cccgcgtcca gcagggatcc gaagaggtga tcctgcgggc
     7021 ccccgtgggc ggggtggtct cctcggttgt cgggatcgag ggcgagccgc tcgtgccggg
     7081 gcaggcgatc ctcgccatcg agccggcgac ggagggctcg tgaccggacg gcacgcccag
     7141 atcgccgggc tcggctccgc cctccccgag cgggtcgtga cgaacgacga cctcgccgcg
     7201 atgggcgtgg acacctcgga cgagtggatc cgcgccagga cggggatcgg agcgaggcga
     7261 ttcgtggccg agggcgagac ggcctcgatg ctgggcgcgc aggccgcgag gaaggcgctc
     7321 gaggcggcgg gggtggatcc gggcgccgtg gacctcacga tctccgccac cttcaccggc
     7381 gaccagcccc tcccctcgac cgcctcgttc ctccaggcgg agctggggat ctacggggcg
     7441 gccttcgacc tcgccgccgc ctgcgcgggg ttcatctacg ccaccgagat ggcgagggga
     7501 cagatcgtca ccggcggggc cgagacggtg ctcgtcgtcg gcgtcgaggt gctctcgccg
     7561 atcctcaacc tggaggatcg cacgacctgc gttctcttcg gcgacggcgc cggcgccgcg
     7621 gtgctcgtcc ccggggacga gccggggatc atcggctcgg tcctcgagaa cgacggtcgc
     7681 cacgcccaca tgctcgaggt tccctccgga gggtcggcct cgcccacctc gtccgagacg
     7741 ctcgcccgcg gcgagcacta tgtgcggatg gccgacgggc gcgaggtgtt caagcgggcc
     7801 gtgcggggga tgtccgacgc cgcgaggcgg ctgctcgaga aggccggcct cgccctcgac
     7861 gacgtcacga tgctcgtccc ccaccaagcc aacgcccgta tcatccgcgc agtggccgag
     7921 cgcctctccg tcccgcccga gcgcgtctac atcgacctgg aggaggtggg gaacacctcc
     7981 gccgcctccg tcccgatcgc cctcgaccac gcgtggcgcg agggcaggct gtcgcccggc
     8041 gacctggtgc tgacagccgc cttcggggcg ggcctggcct ggggagcgaa cctgatccgg
     8101 tggaccgcgg agccgccggc gtgagtgggc gcatcgccct cgtgaccggc gcggggggcg
     8161 gcctgggccg cgccgccgcg ctcgagctcg cgggtcgggg tatggccgtg gcgtgcgcct
     8221 acgccaccaa cgacgcgggg gccaaggaga cggccgagtt gatcgaggag cgcggcggcg
     8281 cggcccaggc cttcggggcc gacgtcgccg acgaggccca ggtacggaac cttttcgccg
     8341 aggtcaagga gtggcgcggg gccccgctcg tcctcgtggc caacgccggc gtccgccgcg
     8401 acggacccac ggtgaagtac ccgctggagc agtggcagag gacgctcgac gtcaacctca
     8461 caggggtgtt cctctgcgtg cgggaggctc tccggggcat gcagctcgcc cggtggggac
     8521 gggtgatcct cgtctcctcc gccgcgggcc tgcggggcaa cccgggccag gcctcctaca
     8581 gcgcctccaa ggccggggtg ctgggcctga cccgctcgct ctcccgggag gtgggcgcgc
     8641 gcgggatcac ggtgaatgcc gttgcgcccg gctacgtcga gaccgcgatc gtggccgaga
     8701 tgaccgaggc ggcccgcgag cggatgatgg ggctgatcgc gaccgggagg ccggggcgcc
     8761 ccgaggagat cgccgccgcg atcggctttc tcgccagcga ggaagcctcc tacgtaactg
     8821 gagcggtgct ccctgtcgac gggggtatga ccgcgtaggg ttgtcgggaa ctggaccaag
     8881 gaggaaggga tggagaggaa ggagatcgag gagcgcgtcc gcaaggtgct ggccgagcag
     8941 ctggcccggg acccggacga ggtcacgctg gaggccagtt tcgaggacga cctcgacgcg
     9001 gattccctcg acctcgtgga ggccgtgctc gcgctcgagg aggagttcgg catcgagatc
     9061 cccgaggagg agatggaggg cgtcaagacc gtgaaggagg cggtcgacct cgccgcgtcc
     9121 aagctggggg tgtccgccta ggtgaccgag cggaaggtcg tcgtcaccgg gatcggcccg
     9181 gtcaccccgg tcggaaccgg gcgcgaggcg ttctgggacg cactcgcctc gggacgatcg
     9241 ggcatcggcg agatcaccct cttcgacgcc tcggagcacc ccgtgcggat cggcggggag
     9301 gtcgagggct tcgatgccgc cgactggatg gaccgcaagg ccgccaagcg caccgatcgg
     9361 gcggtgcatc tcgccgtggc cgcggccaag ctggcgtggg aggacgccgg ccggcctcag
     9421 atcgacccgg agcgcgcggc cgttgtggtc tccacgggcg tgggcggcct ccgctacatg
     9481 gaggagcaga tcaagatcat gaacgatcgg gggcccggga gggtatcggc cttcacggtg
     9541 cccgcgatga tggccaacgc cgccacggga accgtggcga tggagttcgg gttcacggga
     9601 ccgaacgcat gcatcaccac cgcctgcgcg gcgggggccc acggcgtcgg cgaggggtac
     9661 cgctacatca agcacggcat ggccgacctg gccctggccg gaggcacgga ggcggggatc
     9721 accccgctct cgctggccgc gttcgcccag atgcaggcgc tctccaggaa cgccgatccc
     9781 gagcgcgcgt cccgcccctt cgacgccgac cgggacggct tcgtgatctc ggaggcggcc
     9841 tgcgtgctgg tcctcgagga ggaggagcgg gcccggaccc gaggcgcgac gatctacgcg
     9901 gaggtcgcgg gttacgggac ctcggcagac gcgttccaca tcacccagcc cgagccccag
     9961 gggtcgggcg ccgtgatcgc gatgcagatg gcgctgaccg acgccgggga gccggcagag
    10021 gcggtcgact acatcaacgc ccacggcacc tcaacccccc tgaacgacgc cgcggagact
    10081 cgggccatca agaaggcgtt cggcgactac gcatatgagg tcgcggtctc gtccacgaag
    10141 tcgatgacgg ggcacatggt gggcgcgacc ggtgccgccg aggccgccgt ggccgcgctg
    10201 gccgtgcgcg acggcgtgat ccctcccacg gtcaactacg agacgcccga cccggagtgc
    10261 gacctcgact acgtgccgaa cgagaagcgc gagacggacg tcaggctggc gctctcgaac
    10321 gcgttcgggt tcgggggtca gaacgccgtc ctcgcactcc gacggatctg atcagggcct
    10381 gcggacctgt acctcgccgt ccaccaggac gaacgtcgcc cgataggtga cgccctcggc
    10441 acccggggct cccaggggct cgccgcccag cctgagcctc accgcgccgg cgttcgccag
    10501 ctcgaggcgg atccggcggt cggcccggaa cgtcttcacg aaccccggct tccgccgtcc
    10561 gtcctcggcc agctccccgt cgacccgcac gcggatccag gtcctcgcgt ccttcacccg
    10621 gagccggagg acgatctcgt tctccccgcc cgccggggcg gcggcctcgg cggccggggg
    10681 cgaagtctcc gccgggaggg tcacgggcgg gatggatcgc tccacgctcc gcgcggagac
    10741 caccaccagc gctcccatcg cgcccagcga cgcgatccac aggagcgtgg cgatcagctt
    10801 cttgtgcgat cgtcgctcga cgggccgtgg gagccggatc gactggccgt cgccgatccc
    10861 gtgcacggag gcgtagtcgg cgacgaggga ttcggggtcc agtcccagga accccgcgta
    10921 ctcccggagg aaggccctcg cgtacacggg tgcgcggaag gccgagggcg gggcgtccgc
    10981 ctccagggcg cgcaggaacc tgggcttgat cctcgtgtga agcgcggcgt cctcgacgga
    11041 cacgccgcgc cgcgctcgtg cctccttcag gcgggcgccg acccgtgcct tcggagcaac
    11101 cgagatggcc atgcgaacct ctccatccgg tgcccctccc ccgtgggcgc gattataggg
    11161 gcggtcccgg cgaaaggtgg gacctgatca gccgaagaag aggccgagct cgcgcttcgc
    11221 gctctcggga gagtcgctgc cgtggacgag gttctcggtg atgacctcgc cgtagtcgcc
    11281 ccggatcgtg ccggagggcg cgtccttggg atcggtgggt cccatcagct cgcgcaccgc
    11341 cgccacggcg tcctcgccct cgatcctcgc caccaccacc gggcccgacg tgatgaactc
    11401 gacgagctct ccgaagaacg gcttgtcgcg atgctcctcg tagtggcggt cggcgaggtc
    11461 ctggtcgatg cgcatcatcc gaaggcccgt gatgcggagg ccgcgatcct cgatgcgacg
    11521 caggatctcc cccacgagcc ggcgccggac ggcgtcgggc ttcgcaatca cgagcgtctc
    11581 ctgcgtcgtc atctcagcct ttcacgtagc gggggcggac atccgcgaca gtgtacagag
    11641 agccggtcac gaggatcagg tcgtcggggc ccgcggcctt cgtcgccgcg tcgagcgcgg
    11701 cggccacggt cgggtgcggc atcgcgggga tccccgcccg ctcggcgacg gccgtgagac
    11761 ggtcggcgtc catcgcccgc gggtggctcg acgcggcgac gaaggccgac gtcgtccgcg
    11821 aggcgagcac cgaggcgatc ccggccgcgt ccttcgtctc cagcaccgcc agcacgaggt
    11881 agaggtgctc ccagatgaag gcctccccca gcgccgccgc cagcgccgcg gccccatcgg
    11941 ggttgtgcgc cccgtcgagg accacgaggg ggtggcgagc caccacctcg aggcgccccg
    12001 gggagctcgc gccggccagc gcctcgcgga ccgcgtccgc cccaagctcc cggtcgagcg
    12061 tgagctcgac cgcggccacg gccgcggcag cgttgcgggc ggcatgctcg ccgtggagag
    12121 gcagggagag gcccctgtac tcgccgcgca gcccgcggac cgcgaggcgc tggcccccga
    12181 cggccaggtc ccgcaccggg accgtgaagc cgtcgccctc gtggagcagt atggcgccca
    12241 cctcggcggc acgggcctcc aggaccttcc gagcttcggg ccgctgctcc cgcaccaccg
    12301 cccgcgtgcc ctccttcagg accccggcct tctccgccgc aacctcctcg acggtgccgc
    12361 ccagctcggg atggtcgagc ccgatcggcg tgatcaccgc cacctccgac cggatcacgt
    12421 tcgtcgcgtc ccaggagccg cccatcccta cctcgaacac cccgacgtcg acgggcttgt
    12481 cggcgaacca cagggcggcg agagcggcca gcgtctcgaa gtacgtgatc cgctcgccct
    12541 cccgatccac ctcctccagg aagagccgga ggtgggcgta cgtctccgcg aactccccct
    12601 ccccgatcgg gtccccgcag agggtgaggc gctcccgaac ggtgtggagg tgcggggagg
    12661 tcagcgcccc cgcggagacg ccgtgggcgc agagcagccg ctcgacgaga cgcgtggtcg
    12721 tcgtcttccc gttcgtgccg gccacgtgga ccgtcgggta ggcgagctgg gggtcgccca
    12781 tcagctggac cacggcgcgg atccggtcaa gggtggggat catcctcccc tcgccccgcg
    12841 cctctaggtc ggcgacggca tcctcgaagc gcacgtcagt cgcagcccag ctcgtcgagc
    12901 tgctcgcgga gtgccgcctc ctcctccttg agccggctga gcctccgccg ctgctccttc
    12961 acgacctcgg ccggggcctt cgtcacgaag ccctcgttgg ccagcttccg ctcagccttc
    13021 ccggcttcct ctcccagcgc ctcgatccga cctcgaaggc gatcgcactc agcctcggga
    13081 tcgaggaccc cctccagcgg gatgaacacc tcggctccct gcacgtggag gcgcgccgag
    13141 gccgagggag aggaagcctc cccggtcatg acgatggagc cgacccgggc cagcctccgc
    13201 acctcctcgg acagcgtgcc caggatctct cgctgggcgc cgtcgccctc caccctcaca
    13261 tcgagctcga tcgatggcgg cacccggtga tcgctgcgga accggcggac ggccgtgacg
    13321 agatccatgg cgaagcccac tcgaccctca gcctcctcgt cgcgatgagc gggcatgggc
    13381 tcgggccacg gggcgaccat gatcgacccg tccgggacga agcgccgacg gatctcctcg
    13441 gtgacgaaag gcatgatcgg gtggaggagg cgcaggcttc gctcgaggac ccatgccagg
    13501 accgtggcgg cgtcgcgccg accctcctcg gatccctcgt agaaccgcgg cttcgccatc
    13561 tccaggcccc agtcgcagta ctcggaccag aggaaccggt agagggcctg ggccgcgtcg
    13621 gcgaaccggt acgtctccat cgcggcgtca acctccgcgc ggcacgcctc ggcccgggag
    13681 agcagccacc ggtcggtcac ggtgaggcgc tcgggcgacg gcggcgggag atcaccgccg
    13741 tcgaggttcg ctcggacgag gcgcgcggcg ttccagagct tgttggcgaa gtgcttcccg
    13801 gcttcgatct ggtcttcgga gagaggcagg ttctgctgct cgggcgaggc catccgcgac
    13861 agcgcgaagc gaagcgcgtc ggcgccgtac cgctcgatca tgtcgagggg atcgatcacg
    13921 ttccccagcg acttggacat cttgcggccc tgagcatctc ggaccagccc gtggatcgcg
    13981 acggtgtgga agggcaggtc gccggtcagc cgcagccccg acatcaccat ccgggccacc
    14041 cagaggtaga ggatctcgta gccggtcacg aggaccgagg tgggatagaa gtagcggaga
    14101 tcctctgtct cctcgggcca tcccaacgtg gagaacggcc agagctggga cgagaaccac
    14161 gtatcgagga cgtcggggtc ctgctcgatc tcggcgctcc cgcactcggc gcaggcctcc
    14221 ggatcctcga tcgaggcaaa ctcgtgcccg ttcgagcagt accagacggg gatccggtgg
    14281 ccccaccaga gctggcgaga gatgttccag tcccgcaggc cctccagcca ctccaggtat
    14341 cggtccttcc agcggggcgg cgagaaggcg aggcgccccg actcagccgc ctccatcgcg
    14401 gggcccttca gcgggtcgac cttcacgaac cactgctccc ccgacagcca gggctcgatc
    14461 tcggtccggc atcggtagca gtggcccacg gagtggacgt acggccgctc ctctcgcacc
    14521 aggagaccca tctcgtcgat ccggtcccga acggcccgcc gcgcctcgta ccggtccagg
    14581 cctcggaagg cctcgggcac gacgtcgttg agcctggcct cggacgtgag gatgttccgg
    14641 gcctccagcc ccgtccgctc ggcgatctcg aagtcgttgg ggtcgtgcgc gggcgtgacc
    14701 ttgacggcgc cggtgccgaa ggacgggtcg accgcctcgt cggcgacgac agggagctcc
    14761 cgcccgtcca ccgggtgccg caccgaccgg ccgattaggt cccggtaccg ctcgtcgtcc
    14821 gggtggaccg cgatcccggt atcccccagc atcgtctcga tccgggtcgt ggccacctcg
    14881 atgtggccct cgccgtcggc catggggtac cggaacgtga tgagctctcc ctccacctcc
    14941 tcgtgctcca cctcggagtc ggagagcgcc gtctgatccg tggggcacca gttgatgatc
    15001 cgctcgccac ggtagatcag cccgtcctcg tacaggcgca cgaacgccac gcgcacggcg
    15061 tgctggagac cctcgtccat cgtgaagcgc agccggtccc agtcgcacga gctgccgagg
    15121 gccttcatct gctcgacgat ccggcccccg tagcgctccc gccactccca cacccgttcg
    15181 atgaaggcct cacgcccgat cgcctgcttg tcgatgccct cctcaagcag gcgtcggccg
    15241 acgaggacct cggtcccgat ccccgcgtga tcggtgcccg gcagccagag cgcctcgtac
    15301 ccctgcatgc ggcgacggcg gatcagggcg tcctggatcg tgtgatcgag cgcgtgcccg
    15361 atgtggaggg cccccgtcac gttcgggggc ggcagcacga tgcagaaggg ctcgcgctcg
    15421 tcggtcggct cggcgcggaa gtaccctcgc tcgatccact ccgcgtacag acgcgactcg
    15481 acctcggatg gctcgtaggc cgatgccatg gtcatggccg cgagtgtacc gcggcccctt
    15541 ccggccgccc gatcacgggc gcttcactcg gtcccccgaa cggccgatag agagacggag
    15601 gctcccgtga gggagtcgtc ccaaggaaag gctgaggatg gtgcagcgcg agggacccgg
    15661 gcccgggggc gccgctcccg gagcccccgc gccgcccgcc gttgcgggcg gcttgatcgt
    15721 gcgcgtcttc caggagacgc ccgacgtcat cctcatgctg caggtcagcg ccgatggcta
    15781 cctccgctgt cagacggcca tcagctcctt cccgaccgcc ccgagcctcg cggccctgca
    15841 ggggctcacg atcgagcaga tcctcccccc cgagatcctc tcgaggttcg gacgccggtg
    15901 ccaggaggcg accgaacgga agggcccgat ccggtatcag gagcatctcc gcgtggggga
    15961 ctcggacgtg gtcctcgacg cggccatcac gccgatcttc gatggcgagg gggcctcgac
    16021 gcacctcttc tggagcgctc gcaacgtcac cgacgagtgg accacgcgcg agtcgctccg
    16081 caggacggag gagcggctcc ggacgctgga gcgcgagcac gccaaggccc tggcgagccg
    16141 cagcgaggcg ggcgaggccg cgatcgagcg gcgcgggaag gagctcgagg agcgcacgca
    16201 gaaggcggag gagctcgggc ggcagctcga ggagcgcgcg acggagatcg agtcacgcga
    16261 gcgggcgatg gaggagcagc gcgaggctct cgagggccag gcccgcctga tcgagcagcg
    16321 cgagaagacg ctcgaggagc ggatcggcac gctggacgcc gagcgacggt cgatgctcga
    16381 gaaggcaggg acccacgacg agcagcggaa ggcgctggag gacctccggt cgacgctgga
    16441 ggaggagcgg gcctcgatcg acgcccatcg cgccaagctc gagcacgacc gctccgagct
    16501 ggacgaggcg aaggccgagc tcgagaagcg gcgagcgaac gtcgaggcgg agcgggacgc
    16561 gctgagggcc cgcgtcgagg agctggagcg ctccgccggc gagcaggagg agcgggccgc
    16621 cgaggtggag gctcagcgag agctcgtgga gaccgagcgg gagaccctcc aggccctcaa
    16681 ggcttcgttg agcgaggagc gagcctctct cgaggcggag aaggaagggc tggaggccga
    16741 gaaggaggcc ctcgaggccc gcctggcgca ggtcgagtcg cgtcgagaga ccgcagccgg
    16801 gcacgcggac cacctcttcc aggtgtcgac gaccggggtg gcctggagcg acggggacgg
    16861 gaagctccgc gaggtcaacc gggcgctcgc cgcgatgatg aggcgctcgc ccgaggacct
    16921 caccggcatc tccctgcagg acctgctgca cgccgacgag caggacgcga tcgagcgact
    16981 gctcgcggag gctcgagaga gcgaaggggg cgtcgcgcag accgagttgc ggttcgccca
    17041 cgaggacggg gagtcctcgc acgtgcacgt gacgctgacc gcggtccgcg agggggacga
    17101 cggggacgac cggttcctct ggcaggtcgt ggactacacg gagcgccgcg gcctccagga
    17161 gcggctcgcg caccaggccc tccacgactc cctgacgggg ctccccaacc gggcgctgtt
    17221 cctcgaccgg ctctccaggg cgctgcagcg actcgaccgg aaccccggga cggtggccgt
    17281 gatgatcatg gacctcgatc acttcgccca ggtgaacgag cagaacgggc acgaggtggg
    17341 cgaccgtgtt ctggcccacg tcgccgagcg gctcggcgag tcgatccgcg gctccgacac
    17401 ggtggggcga ctcgagagcg acaccttcgg catcctctgc gaggagctca gcgacgccga
    17461 ggacgcgcag ggggtcgccg accgcctccg ccgggccgtc gagggcaggg cgatgatcga
    17521 cgagctgggc gtcgacgtcg gggtcagcat cggcgtcgcg ctcaccgatg agaccaccga
    17581 ccgtccggag accctcctcc gcgacgccac cctcgccgtc cgcgaggcca aggaggccgg
    17641 gcgcgcccgc accgagatct tcgacaggga ccggcgggag gaggccgcgc agcgcttccg
    17701 gacggagagc gacctggctc gcgcgatcga ggaagacgag ttcagggtct tctaccagcc
    17761 gcagatcgat cttcagggcg gcacgatcgt cggggtcgag gtcctggtgc gatggcagca
    17821 gcccgacagc ggcctggtca ccccctcgga gttcatcccc ctggccgagc agaccgggct
    17881 catcgtgccg ctgggggcct ggatcctgga gcaggcgtgc ctccagtcca agcgctgggc
    17941 ctcggtcgcc gggacggagc tcgcgacctg ggtcaacatg tcgcctcgcc agctggccca
    18001 gcccgacctg ccggacatga ccgagaaggt gctcgctcag accggcgccg acccggcgac
    18061 gctctgcgtc gaagtgacgg agggggtcct tctggaggac accggcgcgg cggtcggcaa
    18121 cctccgtgcg ctgaaggagc tcggcctgcg cgtggggatc gacgacttcg ggaaggggtt
    18181 ctcgtcgctc gcctacctca agcggctccc ggtggacatg ctgaagatcg accggacgtt
    18241 cgtgcagggc ctgggacaga gcaaggagga caccgcgatc gcggaggccg tgatcggcct
    18301 ggcacgggcg atggacctca cctcgatcgc agagggtgtg gagacgcccg agcagctggc
    18361 ccggctgcgg gagatgggat gcgacctggc ccagggctat ctcttcgcgt ccccgcagcc
    18421 cgccgaggtg atcgagcagc tgctgaccca ggacctgcgc tggtagagct cgctaggcgg
    18481 agcgctcctt gcgctccggc tcctggtcca tgggaaccag ggtggggttg acgccctcca
    18541 tgacgacctg ctcgtcgatc acgcaccggt ccacgtcgct gcgcccgggg aggtcgtaca
    18601 tcacgttcag gaggacctcc tccatgatgg cccggaggcc gcgggcgccc gtctgccgac
    18661 ggatcgactg ttcggcgatg gcctcgaggg ccccgggggt gaagtcgagt tcgacgccgt
    18721 cgaactcgaa gaacttgtgg tactggcgga cgagggcgtt cttgggcttg gtgaggatgt
    18781 cgatcagggc atccttgtcg agctggtgaa cgtgggtgat gaccggcagg cggcccacga
    18841 actcggggat caggccgtac ttcaagaggt cctcggggag gccgagggcg agcgactcgc
    18901 ccacgtcctt ctcctcgctg gagcggacgt cggccccgaa cccgatgccc ttccgtccga
    18961 tcctcgactc gatgattttc tccagcccgg cgaacgagcc gccgcagatg aagaggatct
    19021 tggtggtgtc gatctggatg aactcctggt ggggatgctt gcggccgccc tggggcggga
    19081 cgctggccgt cgtgccctcg aggatcttca gcagcgcctg ttgaacgccc tcccccgaga
    19141 cgtcgcgcgt gatcgagggg ttctcggcct tgcgcgcgat cttgtcgatc tcgtcgatgt
    19201 agatgatgcc cgtctccgct cgcttcacgt cgtagtcggc agcctggatc agcttcagga
    19261 ggatgttctc cacgtcctcg ccgacgtagc cggcctcggt gagcgccgtg gcgtcggcga
    19321 tggcgaacgg gacgttgagc atgcgagcca gggtctgggc caggagggtc ttgccgcacc
    19381 cggagggacc gagaaggagg atgttggact tctggagctc gacatcgtcc gcgccacctc
    19441 ccccctcgac ctggacccgc ttgtagtggt tgtagacggc gacggacagg accttcttgg
    19501 cctggtcttg cccgatcacg tagtcgttga ggaactcgta gatctcacgc ggcttgggga
    19561 gctcctcgag cttgatctcg gaggtctccg acagctcctc ctcgatgatc tcgttgcaga
    19621 gatcgatgca ctcgtcgcag atgtagacgc cg
//
LOCUS       DASUTZ010000206        10658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_849368, whole genome shotgun sequence.
ACCESSION   DASUTZ010000206 DASUTZ010000000
VERSION     DASUTZ010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10658
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849368"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VF097_09020"
     CDS             <1..409
                     /locus_tag="VF097_09020"
                     /inference="COORDINATES: protein motif:HMM:NF019028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histone H1-like repetitive region-containing
                     protein"
                     /protein_id="HEX6262973.1"
                     /translation="ERTKPSAPLPQRRPAARPPTGRPDEEKPAPEAAPSRPAAKKPAA
                     KKPAAKKPAAKKPAAKKPAAKKPAAKKPAAKKPAASRPSPRSLVMAIPERGTFHRSDC
                     RFVKGRRDTERITLGTAEKRGYSSCGVCKPTEG"
     gene            635..1708
                     /locus_tag="VF097_09025"
     CDS             635..1708
                     /locus_tag="VF097_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262974.1"
                     /translation="MRRELDEIADAAGAEPRTGLDPRRPSPDDVVIVPEGWERPLAYG
                     RVALSPARAVLLLLAPPGLFGWSFEESWAPPDPLTVAIEDVARPESFRAMHAFGFALW
                     THSPGIVKAAERAGVPTSFVGEGRPGPFPEVPPKRTDAVYLEDNRWADLARDVARELG
                     DRVRGIPTLPHDEVLRRIGEARVLVLPSRVEGTSRIQWEARAMGTVPVALSTNPFAHG
                     LDSSGGAVAVASVSEMAGAVLELLGDRDRLAELAALATRTARAQVDWEAFRGRVRDAL
                     DESPEESGRGARAAVGREIEEVLRRADARASEAEAETSRRARELERLSGRRSVRAAVT
                     LADALCRLLARLPWRGRPEAPRR"
     gene            1807..3390
                     /locus_tag="VF097_09030"
     CDS             1807..3390
                     /locus_tag="VF097_09030"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6262975.1"
                     /translation="MAAALLWVLVSSACTEDRRLPEDAASARDVPGGDPCGLPAGYFE
                     RIRNGHDPTRSGNLQIVPEEPHFFGEWLSHSGPWPYLQRVPLLFLGPGVVPARGWIPA
                     PATTADIAPTLAAMMGSTFSAPDGRTLRAAIDDEAPSPRVAVVVVWDSVGVNVLESYP
                     DAWPALRRLSEQGVWFDRATVGTSPTTTGAVHATIGTGAWPRTHRRVGHQYVSGGRIV
                     ESAKEGPADLEAWTLADHWDQERGNRPLAGLVAFKAWHLGLLGHGSFLRGGDRDLAVT
                     LDFEMGRWTLEPPDDAYFRPIPYAGEVDVPEQKIRDLDLEDGRLDGRWLGEPVLERPD
                     DLATTPAYAAWQTDLIQQMVRREGMGDDAITDLIFTNYNQPDLIGHRWSMNSPQMERA
                     VRSLDEELGRLVGFLDEEIGRGGWVLALTADHGATPDPEVSGATILGLPALREAIGER
                     FAPGEDPRGLVTAAAPNELWIDEARLLRDGASLEDVAEHLRRLTIGDVMGPRAGDDAS
                     DPLLAAAFPSRALGEHGCG"
     gene            complement(3459..4778)
                     /locus_tag="VF097_09035"
     CDS             complement(3459..4778)
                     /locus_tag="VF097_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262976.1"
                     /translation="MPPPVDALADGTGRVERVLQRMLAVLGGDRAAWRDANLELRDAM
                     VDHAARTCPYYRNVAPPGTPFSDIPILTKQVIRERHDDLLSEAVPAERRVASKTSGST
                     DGVPLHFMRDAAQGPLEYDAGRRFLLALEGIPADATMVWVATRPAPFPGVSTDGDPPG
                     LIPVPTLGLRRRDVRRLFRRLRKLDRWYLYGHVSALARIAEIARGGRSLRPLAVVTTA
                     ETLTPDTQRTIEDVFVCPVHSWYGSNEFNGFVMGTEPGARRYVYNPFLVHIEVLDEHG
                     NPTDPGAMGRLVMTDLNNYAMPFLRYDTGDLAIADDPEAALPRVGEIQGRSSEVLVLE
                     GGVRLTPILVGWKLFIDHDLGRHVRAWQCAQVDQRTVELRIVWRDGRRAEAELISAMR
                     EVTGERAEVRVRAVEELERLPSGKTWVLRRMIGPQTGGSDVQTPHDE"
     gene            4777..6363
                     /locus_tag="VF097_09040"
     CDS             4777..6363
                     /locus_tag="VF097_09040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEX6262977.1"
                     /translation="MAVSCTNRSTVPAHHRAVWCWLTRPREAGRRVIGVQTSDTPAGR
                     GPRIGIWGTFDLENYGDMLFPLVIEEELARRLPEARLRRLAPVGAGRPNRFDAGRPAE
                     DLGPWSPTRVAELADELDLVVVGGGEIIHTRDELLAPHYGRSPEEMVERAPSRFFIEG
                     LGPDLEREVPVVWTSVGIPFDPEDGEVLRRALVHRPTITVRDEISRSRLEALGLEAQV
                     VPDPILLLPRIWGPDLLERRLRHLRAMDWYPERGWTLIAQGNRAGLPQADETAAAIDR
                     IVEGREDPTVVIVSTGPIHGDDAFAAALKEGLGARTILLPEAGLQDVAAAIAHADGFV
                     GSSLHGNITAAAYGRPHAILGWGGESKLEGFAALLEAPESVARTPQDLPAAFEKMEAR
                     GPRPDVLATLQDRVEKHFDRVAELAREAAARRPGGAGEGEGDALRALGELRDAYEARG
                     RLLALGRWAAADEAATQREEARALRDEVAWLRGTVGDLERDVADRDRRIEEILTSRSW
                     RATAWLRALGRAGRRLLGRG"
     gene            6360..7682
                     /locus_tag="VF097_09045"
     CDS             6360..7682
                     /locus_tag="VF097_09045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6262978.1"
                     /translation="MTVELRAAERPLVSIVMVTYGGWDWPRRALEAVRDHTDAPYEVV
                     IVDNDSPDGTAERLREGVRGARLILHDRNVGFAPAVNRGVLAARGEHICILNPDALVQ
                     PLWLPPLLEAVARPEVGAVVPLFLEPDGRVQEAGSLVDRQGWTFAIGAGGDPRDLALR
                     SARAIDYGSAACLVMRRETFHRVGGLDAAYLPAYVEDLDLAFALRELGLRTVFEPRSS
                     VVHGGTVSSDVATRTRLIEANREVFLDRWSDRLEGRPPLEELDRLPHRLHALRDVLAP
                     VRLLVVAGRLGTAAGEAVIRMARGRLDARVTALAVEVREVEPLVEAGVEVALGAGEPW
                     LEDRLFHFTAILAWGPAGLERWGGPIRRTQPQAVRVYAAPGAPPGTGLPDVDRVALEE
                     AEIVVADEVAGRPGGRLLRLDDPRWPDRLMTMLGAAPPAALRLSAGPA"
     gene            complement(7662..>10658)
                     /gene="ileS"
                     /locus_tag="VF097_09050"
     CDS             complement(7662..>10658)
                     /gene="ileS"
                     /locus_tag="VF097_09050"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX6262979.1"
                     /translation="PGIHHVLSRTFKDVFPRYRTMTGFSVPRKGGWDCHGLPVELEVE
                     KELGLGTKREIEEIGIAEFNRRCRESVSRYVKDWERLTERIGFWLDLSEAYWTMDPDY
                     VQSVWWSLKELHRRGLLAEDFKIVPWCPRCETPLSDHEVSMGYTQVTDPSVFVRFTVT
                     GGDLDGAHLVGWTTTPWTLISNTGLAVHPQATYAVVERDGERLIVAEALRERVLGENA
                     AAVQSLPGERLMGLTYAPLFDTVAGEGTHRVVGADFVSMDDGSGIVHLSPAFGAEDLE
                     IGRREGWPVVNLVDDRGRFTDEAPELVRGRFIKEADPEITADLESRGLLVSSGTLEHT
                     YPLCWRCSTPLIYYARTSWYVLTTRRKADLLAANEKVGWYPEHIKHGRYGNWLENNVD
                     WALSRARYWGTPLPVWRCGEGHDTVVGSLRELSEVAGRDVTSVDPHRPDIDEVEIACP
                     ECGGEARRIPDVIDAWYDSGAMPFAQWGYHPDLGSGEEIFERRFPADFICEAVDQTRG
                     WFYSLMAEGVLLFGESAYRNCLVLGLIVDKDGRKMSKSLGNVIDPWTIVDAEGADALR
                     WYLFTAGPAWQTRRIFPEAVRETLRRFLLTLWNTYSFFVTYATIDDVDPTADPPPRTE
                     RPVLDRWILSRLHATIRASREGLEGFDATSAARAIDAFVDDLSNWYVRRARRRFWDPA
                     GPDDDRDKAAAFATLYECLATVATLLAPFTPFVSEELHQNLVRGTEPGAAESVHLADY
                     PEADPEAIDPALEEAMALARTAASLGRAARTEAKARVRQPLRRAVVHLPGDADALRPL
                     LDLVAEELNVKEVELTSSEEELSGWTAKPSFRELGPRLGRRVQEVAAALEADDGSLAG
                     RLAAGEIVELQLPSGPVELAPGDVELRRRTREGWGVASDGGVTVALDLDLDETLRLEG
                     LAREVVRAVQDARKAAGLEVADRIDLALSADGDVGRAIEAHAGWIEGEVLAVRTMAAD
                     DLESGHRQSAEVEGEPIEIALRRAGG"
BASE COUNT         1465 a         3752 c         3940 g         1501 t
ORIGIN      
        1 cgaacggacc aagccgagcg cgcccctgcc ccagcggcgc cccgccgcgc ggccgccgac
       61 cgggcggccc gacgaggaga agccggcgcc cgaggccgcc ccatcgcggc ccgccgccaa
      121 gaagcccgcc gccaagaagc ccgccgccaa gaagcccgcc gccaagaagc ccgccgccaa
      181 gaagcccgcc gccaagaagc ccgccgccaa gaagcccgcc gcgaagaagc ccgccgcctc
      241 ccgaccgtcc ccccgatcgc tcgtcatggc gatccccgag cgcgggactt tccatcggtc
      301 ggactgcagg ttcgtcaagg gccgtcggga cacggagcgc atcaccctgg ggaccgcgga
      361 gaagcgcggc tactcatcct gtggggtgtg caagcccacc gaagggtagg atgcggcgcg
      421 gggagcggca ctaggggaag gcgatgacct cccgagagcc gcgggtgtga tgccgtacgt
      481 ggtctgcagc cttggagggg acgaagatcc ttggcgccta ccgcgatccc gcttcagcca
      541 gcgcacgtgg atccacggcg gtgagcgaac cctctacgag ctggccgtag cgatcgcgtc
      601 cctcggcgag gaggttgagc tccgcggaga cctcgtgcgt cgggagctcg acgagatcgc
      661 cgacgccgcg ggcgctgaac ctcggaccgg cttggacccg aggcgcccgt cgccggatga
      721 cgtcgtgatc gtcccggagg gctgggagcg acccctggcg tacgggaggg tcgctctctc
      781 tccggcccgg gcagtactcc tcctcctcgc acccccaggg ctgttcggat ggtccttcga
      841 ggagtcctgg gctcctcccg accccctcac cgtggcgatc gaggacgtgg cccgcccgga
      901 gagcttccgg gcgatgcacg cgttcgggtt cgcgttgtgg acgcactcac ccgggatcgt
      961 gaaggcggcc gagcgggccg gggtccccac ctcgttcgtg ggcgagggac ggcccggacc
     1021 cttcccagag gtgccgccca aaaggaccga cgccgtctac ctggaggaca accgatgggc
     1081 cgatctcgct cgggacgtgg ctcgggagct cggcgacagg gtccgaggca tccccaccct
     1141 cccacacgat gaggtccttc ggaggatcgg agaggcgagg gtgctcgtcc ttccttcgag
     1201 ggtagagggc acctctcgca tccagtggga ggctcgagcg atgggcaccg tgcccgtcgc
     1261 cctttcgacg aacccgttcg cccacggcct cgactcctcc gggggagccg tggccgtggc
     1321 gtcggtgtca gagatggccg gggcggtcct cgagctcctc ggggaccgcg accggctggc
     1381 ggagctcgcg gcgctcgcga cccgaacggc ccgagcccag gtcgattggg aggcgttccg
     1441 cggtcgggtt cgagacgctc tcgacgagtc gcccgaggaa tcgggtcgag gggccagggc
     1501 cgccgtcggc cgagagatcg aggaggtcct gcgacgggcc gatgcccggg cgtccgaggc
     1561 cgaggcggag acatcccgtc gcgcgagaga gctggagcgg ctctcgggca ggcgatcggt
     1621 tcgggccgcg gtcaccctcg cggatgcgct gtgccggctg ctggctcgcc tcccatggag
     1681 gggccgaccg gaagctcctc gccgatgagt gagaggtctc cgaggatctc tgggcccccg
     1741 gggcgcatcg accccgtcgg ccgggcgacg tcgtccggcc gccgccgatc gcgcggtcgt
     1801 cgcgtcgtgg ctgccgcgct cctctgggtc ctcgtgtcga gcgcgtgcac cgaggaccgg
     1861 cggctgccgg aagacgcggc ctccgcgcgg gatgtcccgg ggggcgatcc ctgcgggctg
     1921 cccgccggct acttcgagcg tatccgcaac ggccacgatc ccacccgctc ggggaacctc
     1981 cagatcgtgc ccgaggagcc ccacttcttc ggagagtggc tgtcccactc gggcccgtgg
     2041 ccctacctcc agcgggtgcc tctgctgttc ctcggaccgg gcgtggtgcc ggcccgaggg
     2101 tggatcccgg cgccggccac gacggccgac atcgctccga ccctcgccgc gatgatgggc
     2161 tccacctttt cggccccgga cgggcgcacg ctccgggcgg cgatcgacga tgaggcgccg
     2221 tcgccgcggg tcgcggttgt cgtcgtctgg gacagcgtgg gcgtcaacgt gctggagtcg
     2281 tatccggacg cgtggccggc ccttcgccgc ctctccgagc agggcgtctg gttcgatcga
     2341 gccacggtcg gcacctctcc gacgacgacg ggggccgtcc atgccacgat cgggaccggg
     2401 gcgtggcctc gcactcaccg acgagtcggc catcagtacg tgtccggagg tcgcatcgtc
     2461 gagtccgcga aggagggccc cgccgatctc gaggcgtgga ccctcgccga tcactgggac
     2521 caggagcggg gcaatcgccc cctggccggc ctcgtggcct tcaaggcctg gcacctcggc
     2581 ctgctcggcc atggatcctt cctgcgtggc ggcgatcgag accttgcggt gacgctcgac
     2641 ttcgagatgg ggcgatggac cctcgagccg cccgacgacg cctacttccg cccgatcccc
     2701 tacgcggggg aagtggatgt gccggagcag aagatccgcg atctcgatct cgaggacggg
     2761 aggttggacg ggcggtggct gggagagccg gttctggaac gaccggacga tctggcgacc
     2821 acgcccgcct acgccgcatg gcagacggac ctgatacagc agatggttcg gagggaaggc
     2881 atgggggacg atgccatcac ggacctgatc ttcacgaact acaaccagcc cgacctcatc
     2941 ggccatcggt ggagcatgaa cagcccacag atggagcggg ccgtgcggtc tctcgacgag
     3001 gagctcggga ggctggtcgg cttcctcgac gaggagatcg ggcggggggg ctgggtgctc
     3061 gccctgacgg cggatcacgg ggcgacgccc gaccccgagg tgagcggagc gaccatcctc
     3121 ggtctccccg cacttcggga ggcgatcggc gagcggttcg ctccggggga ggatccccgg
     3181 ggcctggtca cggccgctgc gccgaacgag ctctggatcg acgaggcgag gctcctgcgg
     3241 gacggagcct ccctcgagga cgtcgccgag catctccgac gtctcacgat cggggacgtc
     3301 atggggcctc gcgccggaga cgacgcgagc gatccgctgc tcgcagcggc cttccccagc
     3361 cgcgcgctcg gcgaacacgg gtgcgggtga cggtgcgggg tcggtcctga ccgtcgatcg
     3421 cccgcggcgc cggacgaccg cggccgcgag aagcgtctct actcgtcgtg tggggtttgg
     3481 acgtcggatc cgcctgtctg agggccgatc atccggcgca gcacccaggt cttccccgag
     3541 ggcagccgct cgagctcctc gaccgcccgc acccgcacct cggcgcgctc gccggtcacc
     3601 tcccgcatgg cggagatcag ctccgcctcc gctcggcggc cgtcgcgcca cacgatccgc
     3661 agctcgaccg tccgctggtc gacctgcgcg cactgccacg cccgcacgtg cctccccaga
     3721 tcgtgatcga tgaagagctt ccaaccgacg aggatcggcg tcagccggac cccgccctcc
     3781 aggacgagca cttcggaaga gcggccttgg atctccccga cgcggggcag cgccgcttcc
     3841 ggatcgtcgg cgatggccag gtcgccggtg tcgtatcgga ggaacggcat cgcgtagttg
     3901 ttgaggtcgg tcatgacgag gcggcccatc gctccgggat cggtggggtt tccgtgctcg
     3961 tccaggacct cgatgtgaac gaggaacggg ttgtagacgt accggcgcgc gccgggctcg
     4021 gttcccatga cgaacccgtt gaactcgttc gatccgtacc aggagtgcac ggggcagacg
     4081 aagacatcct cgatcgtccg ctgagtgtcg ggggtgagag tctcggccgt ggtgacgacg
     4141 gccagcggtc ggaggctccg cccgcctcgg gcgatctccg cgatccgggc gagggcgctg
     4201 acgtgcccgt agaggtacca gcggtccagc ttccggagtc ggcggaagag ccgccggacg
     4261 tcgcgcctgc ggaggcccag cgttgggacg gggatgagcc cgggcgggtc cccgtccgtg
     4321 gagacccccg ggaagggcgc ggggcgcgtc gcgacccata ccatcgtggc gtcggccggg
     4381 atgccctcga gcgcaaggag gaaccgtcgt ccggcgtcgt actcgagcgg cccctgcgcc
     4441 gcgtcacgca tgaagtggag cggcacgccg tcggtgctcc ctgacgtctt cgaggcgacc
     4501 ctcctctcgg cgggaaccgc ctccgagagg agatcgtcgt ggcgctcccg gatcacctgc
     4561 ttcgtgagga tggggatgtc tgagaagggc gtcccgggcg gcgcgacgtt gcggtagtag
     4621 gggcacgtcc gtgccgcgtg atccaccatc gcgtcgcgga gctcgagatt ggcgtcgcgc
     4681 caggcggccc ggtcgcctcc cagaacggcc agcatcctct ggagcacgcg ctcgacgcga
     4741 cccgtgccat cggcgagagc gtcgactggt gggggcatgg cggtatcttg cacgaaccgg
     4801 agcaccgtgc ccgcccatca ccgcgcggta tggtgctggc tgacccggcc gcgggaggcg
     4861 ggtcggaggg tgatcggcgt gcagacgagc gataccccgg cggggcgagg gcccaggatc
     4921 ggcatctggg ggaccttcga tctcgagaac tacggcgaca tgctcttccc tctcgtgatc
     4981 gaggaggagc tggcgcggcg gctgccggaa gcccgcctgc gcaggctcgc cccggtggga
     5041 gcgggccgcc ccaatcggtt cgacgcgggg aggcccgccg aggacctcgg cccctggagc
     5101 cccacgcgcg tggccgagct cgccgacgag ctcgacctcg tcgtagtcgg gggaggggag
     5161 atcatccaca ccagggacga gctcctcgct cctcattacg ggaggagtcc cgaggagatg
     5221 gtggagcggg ccccgagccg gttcttcatc gaggggctcg gccccgacct cgagcgggag
     5281 gtgccggtgg tctggacctc ggtggggatc cccttcgacc ccgaggatgg ggaggtgctc
     5341 cgtcgggcgc tcgtgcatcg gcccacgatc accgtccggg acgagatctc ccgatcccgg
     5401 ctcgaggcgc tgggcctgga ggcccaggtg gtccccgacc cgatcctcct cctgcccagg
     5461 atctgggggc cggacctgct cgagcggcgg ctgcgacacc ttcgggcgat ggactggtat
     5521 ccggagcggg ggtggaccct gatcgcccag gggaatcggg ctgggctccc gcaggccgac
     5581 gagaccgcgg cggcgatcga tcggatcgtc gagggccggg aggaccccac ggtggtcatc
     5641 gtctcgaccg gcccgatcca cggggacgac gccttcgccg cggcgctgaa ggaaggtctg
     5701 ggggcccgaa cgatcctcct ccccgaggcg gggctccagg acgtggcggc ggcgatcgcg
     5761 cacgccgacg ggttcgtggg ctcgtcgctc cacggcaaca tcacggctgc ggcgtacgga
     5821 cgcccccacg cgatcctggg atggggcgga gagtcgaagc tggaagggtt cgccgcgctg
     5881 ctcgaagccc ccgagagcgt ggccaggacg ccgcaggatc tcccggccgc cttcgagaag
     5941 atggaggctc gcgggccccg cccggacgtc ctcgcgacgc tgcaggatcg cgtggaaaag
     6001 cacttcgatc gggtcgcgga gctggcgcgg gaagccgcgg cccgccgccc cgggggtgcc
     6061 ggcgaggggg aaggagacgc ccttcgcgcg ctgggggagc tgcgggacgc ctacgaggcg
     6121 cgaggtcggc tcctcgcgct cggccggtgg gccgccgcgg acgaggcggc tacccagcgt
     6181 gaggaggctc gggcgctgcg cgacgaggtg gcgtggctgc gtgggacggt gggggacctc
     6241 gagcgggacg tggccgatcg ggatcggcgc atcgaggaga tcctcacgag ccggtcgtgg
     6301 cgggccacgg cgtggctgcg ggcgctcggc cgcgcgggcc gcaggcttct ggggcgggga
     6361 tgaccgtcga gctccgcgcg gccgagcgcc cgctcgtctc gatcgtgatg gtgacctacg
     6421 gcgggtggga ctggcctcgg cgggccctgg aggcggtgcg ggaccatacg gacgctccgt
     6481 acgaggtggt gatcgtcgac aacgactcgc ccgacgggac ggcggagcgc ctccgggagg
     6541 gggtccgcgg ggcgcgcctg atcctccacg accgcaacgt gggcttcgcg ccggctgtga
     6601 accggggcgt gctcgcggcg aggggcgagc acatctgcat cctcaacccc gacgctctgg
     6661 tgcagcccct gtggcttccg ccgctgctcg aagcggtggc gcggcccgag gtgggagcgg
     6721 tcgtgcctct cttcctggag ccggacgggc gtgtccagga ggcagggtcg ctcgtggacc
     6781 gacagggctg gaccttcgcg atcggggcgg gaggcgatcc gcgggacctc gccctccgat
     6841 ccgccagggc gatcgactac gggtccgcgg cgtgcctggt catgcgccgg gagacgttcc
     6901 atcgggtcgg agggctcgac gcggcgtacc tgcccgccta cgtcgaggac ctcgacctcg
     6961 cgttcgcgct gcgcgagctc gggctccgga ccgtgttcga gccgcgctcg agcgtcgtgc
     7021 acgggggcac ggtctcgagc gacgtggcca cccgcacccg cctgatcgag gcgaatcggg
     7081 aggtgttcct cgaccgatgg tccgatcggc tcgaggggcg gccgccgctg gaggagctgg
     7141 accgcctgcc gcaccgcctc cacgccctgc gggacgtgct cgcgcccgtg cggctgctgg
     7201 tcgtggccgg caggctgggc accgctgcgg gcgaggccgt gatcaggatg gcgcggggta
     7261 ggctcgacgc gcgggtgacg gcgctcgcgg tcgaggtccg ggaggtcgag ccattggtgg
     7321 aggccggcgt cgaggtggct ctgggcgcgg gggagccatg gctcgaggat cgactgttcc
     7381 acttcaccgc catcctggcg tggggccccg cgggcctcga gcgctggggc ggcccgatcc
     7441 gaaggaccca gccgcaggcg gtccgcgtct acgcggcccc cggcgccccg cccggcacgg
     7501 ggctgcccga cgtggatcgg gtggcgctgg aggaggccga gatcgtggtg gcggacgagg
     7561 tcgcggggcg gccggggggg cggctcctgc gcctcgacga tccccggtgg cccgatcggc
     7621 tcatgacgat gctcggagcc gcgcccccgg ccgccctccg gctatccgcc ggcccggcgt
     7681 agcgcgatct cgatcggctc gccctccacc tccgcggact gacgatgccc gctctcgagg
     7741 tcgtccgcgg ccatggtccg caccgcgagt acctcgccct cgatccaccc cgcgtgcgct
     7801 tcgatcgccc ggcccacgtc cccgtcggcc gaaagagcca gatcgatccg gtcggccacc
     7861 tcgagcccgg cggccttccg ggcgtcctgg acggcgcgca cgacctcgcg ggccaggccc
     7921 tccaggcgca gggtctcgtc gaggtcgagg tcgagggcca ccgtcacgcc accgtcgctg
     7981 gccacgcccc agccctcgcg ggtgcgccgc ctgagctcga cgtcgccggg ggccagctcg
     8041 accgggcccg agggcagctg gagctctacg atctcgccgg ccgccagccg ccccgccagc
     8101 gacccgtcgt ccgcctccag ggcggcggcc acctcctgga ctcgccggcc tagacgaggg
     8161 cccagctcgc ggaagctcgg cttggccgtc caccccgaca gctcttcctc ggaggaggtc
     8221 agctccacct ccttcacgtt gagctcctcc gcgacgagat cgagcagcgg ccggagcgcg
     8281 tcggcgtcgc ccggcaggtg cacgacggct cgccgcagcg gctgacggac gcgcgccttg
     8341 gcctcggtcc gggccgcccg ccccagcgac gcggccgtcc gggccagcgc catggcctcc
     8401 tcgagcgccg ggtcgatcgc ctcgggatcg gcttccgggt agtcggcgag gtgcaccgac
     8461 tcggcggcgc cgggctcagt cccgcgcacc aggttctggt ggagctcctc ggagacgaac
     8521 ggcgtgaacg gcgccagcag cgtggccacc gtcgccaggc actcgtacag cgtggcgaag
     8581 gccgcggcct tgtcccggtc gtcgtcgggc ccggccgggt cccagaaccg ccgccgtgcc
     8641 cggcgcacgt accagttcga gagatcgtct acgaaagcgt cgatcgccct ggccgccgag
     8701 gtggcgtcga acccctccag cccctcccgc gacgcccgga tcgtggcgtg gagccgggag
     8761 aggatccacc ggtcgagcac cggccgctcc gttcggggcg gcgggtcggc cgtcgggtcg
     8821 acgtcgtcga ttgtggcgta ggtcacgaag aacgagtacg tgttccacag cgtaaggagg
     8881 aacctccgca gggtctcccg aacggcctcg gggaagatgc ggcgcgtctg ccaggccggg
     8941 cccgccgtga agaggtacca ccgcagcgcg tcggctccct ccgcgtccac gatcgtccag
     9001 ggatcaatca cgttccccag ggacttggac atcttgcggc cgtccttgtc cacgatcagg
     9061 ccgagcacga ggcagttccg gtaggccgac tcgccgaaga gcagcacccc ctcggccatc
     9121 agcgagtaga accagccccg cgtctgatcc accgcctcgc agatgaagtc cgcggggaag
     9181 cgtctctcga agatctcctc gccgctcccc aggtcggggt ggtagcccca ttgggcgaag
     9241 ggcatcgctc cggagtcgta ccaggcgtcg atcacgtctg ggatgcggcg ggcctcgcct
     9301 ccgcactcgg ggcacgcgat ctcgacctcg tcgatgtccg ggcggtgagg gtcgacggag
     9361 gtgacgtccc gccccgcgac ctccgagagc tcccggagcg agccgacgac cgtgtcgtgg
     9421 ccctccccgc agcgccacac ggggaggggg gttccccagt agcgggcccg cgagagcgcc
     9481 cagtccacgt tgttctccag ccagttcccg tagcggccgt gcttgatgtg ctccgggtac
     9541 cagcccacct tctcgttggc cgccaggagg tcggccttcc gccgtgtcgt gagcacgtac
     9601 caggaggtcc gggcgtagta gatcagcggc gtggagcatc gccagcagag gggataggtg
     9661 tgctcgagcg ttcccgagga gaccagcagc ccgcgcgact cgaggtcggc ggtgatctcc
     9721 gggtccgcct ccttgatgaa gcggccccgg accagctcgg gggcctcatc cgtgaagcgt
     9781 ccccggtcgt cgacgaggtt gaccacgggc cagccctcgc gccggccgat ctcgaggtcc
     9841 tcggccccga aggcggggga gaggtggacg atccccgacc cgtcatccat cgagacgaag
     9901 tccgccccca cgacgcggtg cgtgccttcg ccggctaccg tgtcgaagag gggggcgtag
     9961 gtgaggccca tgagccgctc gccggggagc gactggacgg ccgccgcgtt ctcgcccagc
    10021 acgcgctcgc gaagcgcctc ggccacgatc agccgctcgc cgtcccgctc caccaccgcg
    10081 taggtggcct gtgggtgcac ggcaaggccg gtgttggaga tcagggtcca gggggtggtg
    10141 gtccagccca cgaggtgcgc cccgtcgagg tccccgccgg tcaccgtgaa ccggacgaag
    10201 acgctggggt cggtgacctg cgtgtacccc atcgagacct cgtggtccga gagcggcgtc
    10261 tcgcaccgcg ggcaccacgg gacgatcttg aagtcctcgg cgagcagccc ccgccggtgc
    10321 agctccttca gcgaccacca gacgctctgc acgtagtcgg gatccatcgt ccagtaggcc
    10381 tcggacaggt cgagccagaa cccgatccgt tcggtgagcc gctcccaatc cttcacgtac
    10441 cgactcaccg actcgcggca ccggcggttg aactccgcga tgccgatctc ctcgatctcg
    10501 cgcttcgtgc cgaggccgag ctccttctcc acctcgagct cgaccgggag gccgtggcag
    10561 tcccagcccc ccttccgggg gaccgagaag ccggtcatgg tccggtaccg ggggaagacg
    10621 tccttgaagg tccgggagag gacgtggtgg atgccggg
//
LOCUS       DASUTZ010000207         4085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_460585, whole genome shotgun sequence.
ACCESSION   DASUTZ010000207 DASUTZ010000000
VERSION     DASUTZ010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460585"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VF097_09055"
     CDS             <1..508
                     /locus_tag="VF097_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262980.1"
                     /translation="TLDPVWEVDEPEVQFYHSALFSDDKTTVIFGDEIIVGHCFDGTG
                     SGQLWFYDLATGDLQGSYNIPRNQGGAYCSAHMFNNVPGVKQDVLVSSWYGGGVTVVD
                     FTDRSNPTEIGYYDPAGGSIWSAYWYNDYIYASDIPAGTRFLQFTEKSPGRERSQPFR
                     EMNPQTQK"
     gene            805..1953
                     /locus_tag="VF097_09060"
     CDS             805..1953
                     /locus_tag="VF097_09060"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEX6262981.1"
                     /translation="MARASRRESSDPPGVSWDRVRGVRLARQHLLRRAPRSRMLDVVR
                     DVCGLHGQVASSTELQVWARIKGSSPEDVREALWERRSLIRTWAMRGTLHVLAAADLP
                     LFVAALRTHNRWWKGTWLRMIGFGAEELREILDAIRDSLGAQPITREKLAERVGERVD
                     HDARERMLSGWGEMLKPAAFEGSLISGPPRGQSVTFVRPDRWLGAWDEPEPEAAWREI
                     VRRYLRTYGPAAREEFARWWGTQPAPAGRVLKASADELTEVDVDGYRGFALTEDLAEL
                     RKASPRAPVRLLPMFDPYVVGIRPRSSLVDPAFEDRVFRSAGWISPVVLVDGMAAGVW
                     NYEQRGGRIEVTVEPFEKLSRAVHKEIEAEADRLGRFLDAPVDVASGT"
     gene            1950..2678
                     /locus_tag="VF097_09065"
     CDS             1950..2678
                     /locus_tag="VF097_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262982.1"
                     /translation="MNEDQLFGLPLDEFTAARDELAAELRGHGERDAAAQVKALRRPT
                     VAAWAVNQLARERAAEVQELLSLREELRAAQESAISGGGVKRMREIGERRRRVIEGLT
                     EQAEEVLTRGGHAASRSTLDRVEDTLMAATLDEEGARAVEAGRLTRELTPPSGFESLT
                     GVLAPPKKKRSPAPDRKARERARRAREEARAAEEEAERADREARELEEEAGRARRRAD
                     RSRRKAEKAAARAAELESKAEDTG"
     gene            complement(2686..>4085)
                     /locus_tag="VF097_09070"
     CDS             complement(2686..>4085)
                     /locus_tag="VF097_09070"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6262983.1"
                     /translation="VASGGWGEHVPVNGPESHPGSPIQPRLGRIASLAARLPRIGADP
                     GDDEDLRQKKALLVLLAVLIAPVAGLWGILYLAFGSPVGIAPLVYLAISLASLLIFAR
                     TRSFRFLLLVQLFDILLAPTAGGQMLVGGFLPSGGVALWGILAPLGALVFLEVRQAVR
                     WFVGFLVVFVVTGVLGELVFPDADLPAWFTSTMLALNVIGAGSIAFTLLASFANQRDA
                     ALRGLRAEQAKAEALLVNVLPSSIAQRLKGASRSIADYFESASILFADVVDFTPLARR
                     LAPAEVVGILDELFSHFDVLVERHGLEKIKTIGDCYMAASGVPDPTPDHARRAALLAL
                     DMRDAVAAWAVAGRLGLELRIGINSGPVVAGVIGTKRFLYDLWGDAVNIASRMETNGA
                     PGEIQVTRATYELLKDQFVCERRGAIRVKGQGLMETWYLVGPRSDEVSESDAPGSGER
                     SIRAEPSAAERPASS"
BASE COUNT          654 a         1404 c         1470 g          557 t
ORIGIN      
        1 gaccctggat cccgtgtggg aggtggacga gccggaggtg cagttctacc actcggccct
       61 gttcagcgac gacaagacca ccgtgatctt cggcgatgag atcatcgtgg ggcactgctt
      121 cgatgggacc gggagcggcc agctctggtt ctatgatctc gccacggggg atctccaggg
      181 ctcctacaac atcccgcgga accagggggg cgcgtactgc tccgcccaca tgttcaacaa
      241 cgtgcccggg gtgaagcagg acgtcctcgt gtcgtcctgg tacggcgggg gcgtgaccgt
      301 cgtcgacttc accgaccgta gcaacccgac cgagatcggc tactatgatc ccgcgggcgg
      361 gagcatctgg tcggcgtact ggtacaacga ctacatctac gccagcgaca tcccggccgg
      421 gacgcgattc ctccagttca cggagaagtc gccgggccgg gagcggtccc agccgttccg
      481 ggagatgaac ccgcagacgc agaagtagct cggggggccg aggccgtgcg cggctgacgg
      541 gtcggcctgg ccaagcccgg cgctacgcga cgcgcccgcc gcgcggctcg accagccctc
      601 caggggcccg tctgtgcagt tcccacgaga gcctcctctc ggggtccagc atccaaccgc
      661 gatcgaggct ttcgccgtct ccacaatcct ctggaaactc caccgagacc gtcttcccgg
      721 gccctcgctc gacgacgatc ttgcctcgcg cgacttcgat ctcccctcgg gtcgtggagt
      781 ccccggcgcg ctatcgtcct tcggatggca cgcgcctctc ggagggagag ctcggatcca
      841 cccggcgtct cgtgggatcg cgtgcgaggg gtccgcctcg cgcgccagca cctgctgcgc
      901 cgcgcccccc gctcccgcat gctcgatgtg gtccgagacg tgtgcgggct gcacgggcag
      961 gtggcgtcct ccaccgagct gcaggtctgg gcccggatca agggctcctc gcccgaggac
     1021 gtccgagagg cgctctggga acgccgatcg ctcatccgca cgtgggcgat gcgaggcacg
     1081 ctgcacgtcc tggccgcggc ggacctcccc ctgttcgtcg cggcgctccg cacgcacaat
     1141 cgatggtgga agggcacctg gctgcggatg atcggcttcg gggcggagga gctccgcgag
     1201 atcctcgacg cgatccggga ctccctcggc gcccagccca tcacgcgcga gaagctcgcg
     1261 gagcgcgtcg gcgagcgcgt ggaccacgac gcccgcgagc ggatgctctc cggatgggga
     1321 gagatgctga agccggccgc gttcgaggga tcgctgatct ccgggcctcc ccggggccag
     1381 agcgtgacgt tcgtccgccc cgaccgctgg ctcggggcct gggacgagcc ggagccagag
     1441 gcggcctggc gggagatcgt tcgccggtat ctacggacct acgggcccgc cgcccgcgag
     1501 gagttcgcac ggtggtgggg tacacagccg gctccggcgg ggcgcgtgct gaaggcctcg
     1561 gccgatgagc tgaccgaggt ggatgtggac gggtatcgag ggttcgcgct gaccgaggac
     1621 ctcgccgagc tgcggaaggc atcgccccgg gccccggtgc gtctcctccc catgtttgac
     1681 ccgtacgtcg tgggcatacg gccgcgctcg tcgctggtgg accccgcctt cgaggaccgc
     1741 gtgttccgct ccgccggctg gatatcgccg gtcgtgctcg tggacgggat ggcggccggg
     1801 gtctggaact acgagcagcg cgggggtcgc atcgaggtca ctgtcgagcc gttcgagaag
     1861 ctgtcgcggg ccgtccacaa ggagatcgag gccgaggccg atcgcctggg gaggttcctc
     1921 gacgcccccg tagacgtagc atcgggcacg tgaacgagga tcagctcttc ggccttcccc
     1981 tggacgagtt caccgcggcg cgagacgagt tggccgcgga gctccgcggc catggggaac
     2041 gcgacgccgc cgcgcaggtc aaggccctcc gccgaccgac ggtggccgcg tgggcggtga
     2101 accagctcgc ccgcgagcgc gccgccgagg ttcaggagct cctctctctg agggaggagc
     2161 tgcgggcggc gcaggaatcg gcgatctcgg gcggcggcgt gaagcgcatg cgagagatcg
     2221 gcgagcgtcg ccggcgcgtg atcgaggggc tcacggagca ggctgaggag gtgctgaccc
     2281 ggggcgggca cgccgcgagc cggtcgaccc tcgatcgggt cgaggacacg ctgatggcgg
     2341 cgaccctcga cgaggagggc gcacgggccg tcgaggccgg gcggctgacg cgcgagctga
     2401 cccctccctc cggcttcgag tcgctgacgg gggttctggc cccgcccaag aagaagcgct
     2461 cccccgcccc ggaccgaaag gcccgagagc gcgctcgacg ggcaagggag gaagcccggg
     2521 ccgcagagga ggaggcggag cgggcggacc gcgaggctcg cgagctggag gaagaggccg
     2581 gtcgggcccg aaggcgggcc gaccgttccc ggagaaaagc cgagaaggcc gcggcgcggg
     2641 ccgcagagct cgagtccaag gccgaggaca cggggtagca gggtcttacg acgatgcagg
     2701 gcgttcggcc gccgacggtt cggcccggat cgatcgttcc ccgcttcccg gagcgtccga
     2761 ctcggagacc tcatccgatc gaggccccac gaggtaccag gtctccatca gcccctgacc
     2821 cttgacccgg atcgctcccc gccgttcaca gacgaactgg tccttcagca gctcgtacgt
     2881 cgcacgcgtg acctggatct ccccgggcgc cccgttggtc tccatccgac tcgcgatgtt
     2941 cacggcgtcg ccccagaggt cgtagaggaa gcgcttggtg ccgatcaccc ctgcgacgac
     3001 cggtccggag ttgatgccga tcctcagctc gagccccagg cggccggcaa ccgcccacgc
     3061 ggcgacggca tcccgcatgt cgagggcgag gagcgcggcc ctgcgcgcgt ggtcgggggt
     3121 gggatcgggc acacccgatg cagccatgta gcagtctccg atcgtcttga tcttctcgag
     3181 cccgtgtcgc tcgaccagga cgtcgaagtg ggagaacagc tcatcgagga tcccgacgac
     3241 ctcggccggc gccaggcgcc gcgcgagcgg cgtgaagtcc acgacgtcgg cgaagaggat
     3301 ggaggccgac tcgaagtagt cggcgatcga gcggctcgct cccttcagcc gctgcgcgat
     3361 cgagctcggc aggacgttca cgaggagcgc ctcggccttc gcctgctcgg cccgcagccc
     3421 ccgcagcgcc gcgtcgcgct gattggcgaa cgacgcgagg agggtgaacg cgatggagcc
     3481 ggcgccgatg acgttcagcg ccagcatcgt gctggtgaac cacgccggga gatcggcgtc
     3541 gggaaagacc agctccccga ggacgcccgt gaccacgaag acgaccagga acccgacgaa
     3601 ccaccggacg gcctgtcgca cctcgaggaa gacgagggca cccagcggag ccaggatgcc
     3661 ccacaaggcc acccccccgg aggggaggaa cccgcccacg agcatctggc cgccggccgt
     3721 aggcgcgagg aggatgtcga agagctggac gaggaggagg aaccgaaagc tcctcgttcg
     3781 agcgaagatc agcagggacg cgagcgagat ggcgaggtag acaagcggcg cgatcccgac
     3841 cggcgacccg aaggccaggt acaggatgcc ccacagcccc gccaccggag cgatcaggac
     3901 cgcgagcagg acgaggagcg ccttcttctg acgcagatcc tcgtcgtccc cgggatccgc
     3961 gccgatcctc ggcagccgcg ccgccaggct ggcgatccgg cccagccgcg gctggatggg
     4021 tgaccccggg tggctctccg gtccattcac tggcacatgt tccccccaac cgccggacgc
     4081 gacaa
//
LOCUS       DASUTZ010000208         2989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_460719, whole genome shotgun sequence.
ACCESSION   DASUTZ010000208 DASUTZ010000000
VERSION     DASUTZ010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460719"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VF097_09075"
     CDS             complement(<1..357)
                     /locus_tag="VF097_09075"
                     /inference="COORDINATES: protein motif:HMM:NF013483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEX6262984.1"
                     /translation="MKRRRQQAILELVRREPLSSQRAILDRLREEGFEVTQPTVSRDL
                     DELGLARVRDDSGRLRYAAPQEAAPVGREEQLRHVLQEFALAMVASGNLVVVRTPPGA
                     ANAVAQVLDRVEVPDVI"
     gene            complement(354..1529)
                     /locus_tag="VF097_09080"
     CDS             complement(354..1529)
                     /locus_tag="VF097_09080"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002874.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HEX6262985.1"
                     /translation="MTTPPPLVDRAEGILMDTYARFPATFVRGEGIRLFDDEGREYLD
                     FAAGIAVVQIGHAHPRWVEAVTDQAARLAHVSNLFSTVPQVDLAERLVGLLGWGRAFF
                     ANSGAEANEAALKLVRKATGRSKVVATGGGFHGRTLATLAATGQPEKHAPFEPLPREF
                     VHVPFGDAEALRAAVDERTAAVLLEPVQGEAGVVPAPKGYLAAARDACEGAGALLVLD
                     EVQTGIGRCGAWFAHQLEDLEPDVVTLAKGLAGGLPIGVCLATQEVSAAFGPGDHAST
                     FGGGPIPCAAALAVLDVIEEEGLLERARIMGERLVETLRETAAAAVDVRGRGLLVGVE
                     LPAEIPARQVVSAALGRRLVLTQAGRNTVRFTPPLIATAQDVDEAARIFGAVLEDET"
     gene            complement(1526..2332)
                     /gene="argB"
                     /locus_tag="VF097_09085"
     CDS             complement(1526..2332)
                     /gene="argB"
                     /locus_tag="VF097_09085"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006545430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX6262986.1"
                     /translation="MREHGGAVIVVKLGGSAMSDPGLLETFALDVALLRQVGVRPVIV
                     HGGGPQITRASERLGLTPRFIRGLRVTDAETLEVVRTVLVGRINKDLVKAMNERGAPA
                     AGVSGEDADLMIATRRSEDLGLVGSITEVRPALLGSLMEDFVPVVASLATDGAGQSLN
                     VNADEAAAALAVALGASKLVYLTDVPGIIEDRDGDAALVSEASVAECERLLSSGAVTD
                     GMIPKLEHLIHALRGGVQRGHVLDGRVPHAMILELFTPEGLGTMVTREEA"
     gene            complement(2407..>2989)
                     /locus_tag="VF097_09090"
     CDS             complement(2407..>2989)
                     /locus_tag="VF097_09090"
                     /inference="COORDINATES: protein motif:HMM:NF014065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HEX6262987.1"
                     /translation="DDLGEIVRRDLAPVFNALTVDGCASTNDTVLVMASGAAGPASPD
                     ALGAALREASLDLARKVVAEAEGASKTLVVHVSGAADDEQARHAGRAVAGSLLVKTAL
                     FGEDPNAGRLLQAIGAAGVSLDPSAADVDLAGHRVVEGGVVAAFDQRACREDLKEREV
                     VVRAHLGVGEGEATFFGCDLGYEYVRINAEYRT"
BASE COUNT          397 a         1146 c         1062 g          384 t
ORIGIN      
        1 gatcacgtcg ggcacctcga cccggtcgag cacctgggcc acggcgttcg ccgcccccgg
       61 cggcgtacgg acgacgacga ggttgcccga ggccaccatg gcgagcgcga actcctggag
      121 gacgtgccgg agctgctcct cccgtcccac gggggcggcc tcctggggcg ccgcgtagcg
      181 gaggcgcccg gagtcgtctc ggacccttgc cagccccagc tcgtccaggt ccctcgagac
      241 cgtgggctgc gtgacctcga acccctcctc ccgcaggcga tccaggatcg cccgctggga
      301 gctgagcggt tcccggcgca ccagctccag gatcgcctgc tgccgccgcc tcttcatgtc
      361 tcatcctcca ggaccgcgcc gaagatgcga gcggcctcgt ccacgtcctg ggccgtcgcg
      421 atgaggggcg gggtgaagcg caccgtgttg cggcccgcct gggtcagcac gagcctccgc
      481 cccagggccg cggagacgac ctgccgcgcg gggatctcgg cggggagctc cacgccgacc
      541 agcaggccgc ggccgcgcac gtcgaccgcc gccgcggccg tctcgcggag cgtctccacc
      601 aaccgctccc ccatgatccg ggcccgctcc agcaggccct cctcctcgat cacgtcgagc
      661 accgcgaggg cggcggcgca cgggatcggc ccgccgccga aggtcgaggc gtggtccccc
      721 ggcccgaagg ccgccgagac ctcctgcgtc gccaggcaga cgccgatcgg gaggccgccg
      781 gccagcccct tggccagcgt gacgacgtcg ggttccaggt cctcgagctg atgggcgaac
      841 caggccccgc agcggccgat gccggtctgg acctcgtcca ggacgagcag cgcgcctgcg
      901 ccctcgcagg cgtcgcgggc cgccgccagg tagcccttcg gcgccggcac gacgccggcc
      961 tccccctgca ccggctccag gagcacggcg gccgtccgct cgtcgaccgc ggccctcagc
     1021 gcctccgcgt cgccgaacgg cacatggacg aactcgcggg gcagcggctc gaacggggcg
     1081 tgcttctccg gctgacccgt ggccgcgagc gtggccaggg ttcggccgtg gaacccgccg
     1141 ccggtggcca cgaccttgga ccggccagtc gccttgcgga cgagcttgag agccgcctcg
     1201 ttcgcctcgg cgcccgagtt ggcgaagaac gccctccccc atccgaggag gccgacgagg
     1261 cgctcggcca ggtccacctg gggcacggtg gagaagaggt tgctgacgtg cgccagacgg
     1321 gcggcctggt ccgtcaccgc ctcgacccat cgagggtggg cgtgaccgat ctggaccacg
     1381 gcgatgcccg ccgcgaagtc caggtactcg cgaccctcgt cgtcgaacag ccggatcccc
     1441 tcgccgcgca cgaacgtcgc ggggaaccga gcgtacgtgt ccatcaggat gccttcggct
     1501 cggtcgacga gcggaggagg ggtcgtcacg cctcctccct cgtcaccatc gtgcccaggc
     1561 cctcgggcgt gaacagctcg aggatcatcg cgtgggggac ccgcccgtcg aggacgtggc
     1621 cccgctggac gcccccccgg agggcatgga tcaggtgctc gagcttgggg atcatgccgt
     1681 cggtcaccgc gcccgaggag aggaggcgct cgcactcggc caccgacgcc tccgagacca
     1741 gcgcggcgtc tccatcccga tcctcgatga tccccgggac gtcggtcagg tagacgagct
     1801 tcgaggcccc gagcgccacg gcgagcgcgg cggcggcctc gtcggcgttg acgttgagcg
     1861 actggccggc gccgtcggtg gcgagggaag ccacgaccgg gacgaagtcc tccatcagcg
     1921 acccgaggag ggcggggcgg acctcggtga tcgagcccac cagcccgagg tcctcgctcc
     1981 gccgcgtggc gatcatgaga tccgcgtcct cgccggagac gcccgcggcc ggggcccccc
     2041 gttcattcat cgccttcacc aggtccttgt tgatgcgccc cacgaggacc gttcggacga
     2101 cctcgagggt ctcggcgtcg gtgacgcgga gccctcggat gaaccgaggg gtgaggccca
     2161 ggcgctcgga cgctcgcgtg atctgcgggc cgcccccgtg gacgatcacc gggcggaccc
     2221 ccacctggcg gagcagggcc acgtccaggg cgaaggtctc cagcaggccg gggtcgctca
     2281 tcgccgaccc cccgagcttg acgacgatca ccgcgccgcc gtgctcgcgc atgtagggga
     2341 gggcctcgag cagcacgcgg cccttggcca cggtctcctc gcggggccgg gtggcggcgc
     2401 ggcggctcac gtccggtact ccgcgttgat ccgcacgtac tcgtacccga ggtcgcatcc
     2461 gaagaaggtg gcctcgccct ctcccacacc gaggtgggcc cggaccacca cctctcgctc
     2521 cttcaggtcc tcccggcacg cgcgctggtc gaacgcggcc accacgcccc cctccaccac
     2581 gcggtggccc gcgaggtcga cgtccgcggc cgaggggtcg agcgacacgc cggccgcgcc
     2641 gatcgcctgg aggagacgcc ccgcgttggg gtcctcgccg aacagggccg tcttcaccag
     2701 cagcgagccg gcgacggcgc ggccggcgtg tcgcgcctgc tcgtcgtcgg cggcgcccga
     2761 gacgtggacg acgagcgtct tgctcgcgcc ctccgcttcc gcgacgacct tgcgggccag
     2821 atcgagcgag gcctcgcgca gcgcggcccc cagcgcgtcg ggcgacgcgg gtcccgccgc
     2881 gccgctcgcc atgacgagca ccgtgtcgtt ggtgctggcg cagccgtcca ccgtgagggc
     2941 gttgaagacg ggggcgaggt cgcggcggac gatctctccg agatcgtcc
//
LOCUS       DASUTZ010000209         4669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_720408, whole genome shotgun sequence.
ACCESSION   DASUTZ010000209 DASUTZ010000000
VERSION     DASUTZ010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720408"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VF097_09095"
     CDS             <1..568
                     /locus_tag="VF097_09095"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEX6262988.1"
                     /translation="IVFRLIERMGGGMSDEDAKSLYAGVVTDTGRFQYEATTPETLRV
                     GAALRERDFDHARLAQALYEDGSFAALGVMGKALERAQLDRDLRLVWTWITRKDLEEA
                     GVQIQETDDMIDAIRVAREADVAVVLKEQRDGGFKVSLRSRGDTNVGAVAEAFGGGGH
                     RLAAGYSWEGGLDEAVQALRKTLREAAT"
     gene            579..1424
                     /gene="truB"
                     /locus_tag="VF097_09100"
     CDS             579..1424
                     /gene="truB"
                     /locus_tag="VF097_09100"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006543550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX6262989.1"
                     /translation="MSAPEGIVLVDKPKGITSHDAVDAVRRALGTRKVGHAGTLDPMA
                     TGLLVMGVGRATRLLRFLSALDKEYEGTGRLGVETDTLDAEGEVVRESPVDVGERVVR
                     EAMASLTGEIDQRPPAYSAVRVAGERLYRAARRGEAVEAPPRRVRVEAFELVRYEAPE
                     LDFRVVCSSGTYVRSLVADLGARLGCGAHLTRLVRTRIGPYRIAEAALPEGVEAALPI
                     ERAVAHLPRVELEEEEARAARHGRALGPSGHDGPHAVFDADRRLIGVWKDEGPRARPL
                     VVLAG"
     gene            1477..2400
                     /locus_tag="VF097_09105"
     CDS             1477..2400
                     /locus_tag="VF097_09105"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX6262990.1"
                     /translation="MEVITELEAVRPDGPSAVTIGFFDGVHRGHQAVIHRLVAAAEER
                     DLRPVAVTFDRHPREVLTPSDVPGLLTTTARKAGLIEELGVDLLVVMPFDAEVASWPP
                     ERFVDDVLVERLGVAHATVGANFTFGHRAAGTVEVLTDLGRARGFSVEGVGLLELEGR
                     RISSTSIREALAAGELEWPRVALGRRHVIDGVVVSGAGRGDDLGWPTANLQTDPRVLL
                     PARGVYAGRGLLEEGAWPAAINVGINPTFGEEPLHVEAFLLGFEGEIRGRELAIELWE
                     RLRDEERFESAEALSRQIERDVERTRAVVSE"
     gene            2442..2708
                     /gene="rpsO"
                     /locus_tag="VF097_09110"
     CDS             2442..2708
                     /gene="rpsO"
                     /locus_tag="VF097_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX6262991.1"
                     /translation="MITKEEKQGLIAEYRQGEGDTGSPEVQVALLTKRIEELNEHLKT
                     HRKDHHSRRGLLQMVGKRRRLLEYLKRDDIERYRALIGRLGLRR"
     gene            2759..>4669
                     /locus_tag="VF097_09115"
     CDS             2759..>4669
                     /locus_tag="VF097_09115"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006543547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX6262992.1"
                     /translation="MEPITVSGKVGGTDLSFEAGRLAKLAHGAAVVTLGETQVLVTCA
                     WGAPREGIDFFPLTVDVEERMYAAGKIPGGFFRREGRPSEQAILTARLTDRPLRPTFR
                     EGFRQEVHVVSTILSADQANPYDIITINGASAAVMLAGLPFDGPIGAVRVAMISGRWV
                     ANPTYQDLDLATFDMVVAGRRNDAGGTDILMVEAEAPEEALSHMSEGQPAPTEEVVAE
                     GLEEAKRAIGEAIDLQEELVRRAGAEPKEFPVVADYEDEVMEAVREVAGSALSDALTI
                     ADKTERNERLDAIKEDLAASLAERWGEETYEERARQVKAAFKALQKEIMRKRVIEEGI
                     RIDGRKADEIRPIHCAVDLVPRAHGSGLFERGETQVLNVATLGMLRMTQMIDTLDPEE
                     EKRYMHHYNFPPFSTGEPGFMRGPKRREIGHGALAERALASVIPNEEEFPYALRLVSD
                     VLASNGSTSMASACASTLSLMDAGVPIKAPVAGIAMGLIAEGDRFVTLTDILGAEDAL
                     GDMDFKVAGTKDFITALQLDTKVTGLPAEVLSGALKQAREARLFILDRMLEAIPEPRS
                     ALSAKAPRVEAIQIPVDKIGEVIGPKGKRINEIIAETGADVDIHDDGTVFIGSKEGEG
                     AERAKEMINAIVN"
BASE COUNT          697 a         1557 c         1797 g          618 t
ORIGIN      
        1 gatcgtcttc cggctgatcg agcgcatggg aggcgggatg tcggacgagg acgcgaagtc
       61 gctgtacgcg ggcgtcgtga ccgacaccgg gcggttccag tacgaggcga ccacgcccga
      121 gacgctccgg gtgggggcgg ccctccggga gcgggacttc gaccatgccc ggctggccca
      181 ggccctctac gaggacggct cgttcgctgc gctcggcgtg atggggaagg ccctggagcg
      241 ggcccagctg gaccgagatc tccgtctggt ctggacgtgg atcacgcgga aggatctcga
      301 ggaggccggc gtccagatcc aggagaccga cgacatgatc gacgcgatcc gggtcgcccg
      361 ggaggcggac gtcgccgtgg tgctcaagga gcagcgcgac ggcggcttca aggtctcgct
      421 ccgctcccga ggcgatacga acgtcggggc cgtggccgag gccttcggcg ggggaggaca
      481 ccgcctcgcc gccggctact cgtgggaggg cggcctcgac gaagccgtcc aggccctgcg
      541 gaagaccctg cgcgaggcgg cgacctaagg cagccgtcgt gagcgccccc gaggggatcg
      601 tcctcgtcga caagccgaag gggatcacgt ctcacgacgc cgtggatgcg gtccggcggg
      661 cgctgggcac gcggaaggtg gggcatgcgg ggaccctcga tccgatggcg acggggctcc
      721 tcgtgatggg cgtgggacgg gccacgcgtc tcctgcggtt cctgtcggcc ctggacaagg
      781 agtacgaggg gaccggccgg ctgggcgtgg agaccgacac gctggacgcg gagggcgagg
      841 tcgtccggga gtccccggtg gacgtgggcg agcgcgtggt gcgcgaggcg atggcctcgc
      901 tcacgggcga gatcgaccag cgcccgcccg cgtactccgc cgtgcgggtg gcgggtgagc
      961 ggctgtacag ggcggctcgc aggggggagg ccgtggaggc cccgccccgg cgggtccgcg
     1021 tggaggcctt cgagctggtg cggtacgagg cgcccgaact ggacttccgg gtggtgtgct
     1081 caagcggcac ctacgtgcgc tcgctcgtcg cggatctggg cgcgcgcctc gggtgcggcg
     1141 cccacctgac ccggctcgtc cgcacccgga tcggtcccta tcggattgcc gaggccgcgc
     1201 ttccggaggg ggtggaggcg gccctcccga tcgagcgcgc cgtggcccac ctgccgcgcg
     1261 tggagctcga ggaggaggag gcgcgagcgg cgcggcacgg gcgggccctc gggccctccg
     1321 gccatgacgg tcctcacgcc gtcttcgacg ccgacaggcg gctgatcgga gtttggaagg
     1381 acgagggccc gagggcccgc ccgctcgtgg tcctggccgg ctgaggccgc gcggagcggt
     1441 ccgcgagggg cggcctgcgc taccctcact cccgcgatgg aggtgatcac ggagcttgag
     1501 gccgtccgcc cggacggtcc gtccgccgtc acgatcgggt tcttcgacgg cgtccaccgc
     1561 ggccaccagg cggtgatcca ccgcctggtg gccgcggccg aggagcggga cctgcggccg
     1621 gtggccgtca ccttcgaccg ccacccgcgc gaggtcctca cgccgagcga cgtccccggc
     1681 ctcctgacca ccacggctcg gaaggccggc ctgatcgagg agctcggggt tgacctcctc
     1741 gtcgtgatgc ccttcgacgc cgaggtggcc tcctggcccc cagagcggtt cgtggacgac
     1801 gtgctcgtgg agcggctggg cgtggcgcac gccacggtcg gcgcgaactt caccttcggg
     1861 caccgagccg ccgggacggt cgaggtcctg accgacctgg ggcgggctcg gggtttctcc
     1921 gtcgagggcg tgggcctcct cgagctcgag gggcgcagga tctcctcgac ctcgatccgc
     1981 gaggccctgg ccgccgggga gctcgagtgg cccagggtcg cgctcggccg ccgccacgtg
     2041 atcgacggcg tcgtcgtgtc gggggcgggt cggggcgacg acctggggtg gccgacggcg
     2101 aacctgcaga cagaccctcg ggtgctgctg ccggcccggg gcgtctacgc ggggcgcggc
     2161 ctgctcgagg agggcgcctg gcccgcggcg atcaacgtgg ggatcaaccc cacgttcggc
     2221 gaggagcccc tccacgtgga ggcgttcctg ctcggcttcg agggggagat ccggggccgg
     2281 gagctcgcga tcgagctctg ggagcggctc cgcgacgagg agcggttcga gtcggccgag
     2341 gcgctctccc gccagatcga gcgggacgtg gagcggacgc gggccgtcgt gtccgagtag
     2401 cctccggagc ccgagaggcc gtgtgctacc ctggtcgacg gatgatcacg aaggaagaga
     2461 agcaagggct catcgccgag taccggcagg gcgagggcga cacggggtcg cccgaggtcc
     2521 aggtggcgct cctcaccaag cggatcgagg agctgaacga gcatctcaag acccaccgga
     2581 aggaccacca ctcgcgccgc ggcctcctcc agatggtggg gaagcgccgg cggctcctcg
     2641 agtacctcaa gcgcgacgat atcgagcggt accgcgcgct gatcggccgg ctcgggctcc
     2701 ggcggtagga gggagctcct accgcttctc gagcagccca cgaactcgag gaggaaccat
     2761 ggaacccatc accgtctccg gcaaggtcgg gggcaccgac ctgtcgttcg aagcaggccg
     2821 tctcgccaag ctcgcgcacg gcgccgcggt cgtcaccctt ggcgagaccc aggtgctcgt
     2881 cacctgcgcc tggggcgccc cgcgggaggg catcgacttc ttcccgctga ccgtggacgt
     2941 cgaggagcgg atgtacgccg ccgggaagat ccccggcggc ttcttccgtc gggaggggcg
     3001 gccctccgag caggcgatcc tcacggcgcg gctgaccgac cgccccctgc ggccaacgtt
     3061 ccgcgagggt ttccgccagg aggtccacgt ggtctcgacg atcctctcgg cggaccaggc
     3121 caacccctac gacatcatca cgatcaacgg cgccagcgcg gcggtcatgc tggccgggct
     3181 gccgttcgac ggccccatcg gggccgtgcg cgtggccatg atcagcggcc gctgggtcgc
     3241 gaaccccacc taccaggacc tcgacctcgc gaccttcgac atggtggtcg cgggccgccg
     3301 gaacgacgcg ggcgggacgg acatcctcat ggtggaggcg gaggcgcctg aggaggcgct
     3361 ctcccacatg tcggagggcc agcccgcccc gaccgaggag gtcgtggccg agggcctcga
     3421 ggaggccaag cgtgcgatcg gcgaggcgat cgaccttcag gaggagctcg tccggagggc
     3481 gggggcggag ccgaaggagt tccccgtcgt ggcggactac gaggacgagg tcatggaggc
     3541 cgtccgggag gtcgcgggct cggccctctc cgacgcgctg acgatcgccg acaagaccga
     3601 gcgcaacgag cggctggacg cgatcaagga ggacctcgcg gcgagcctcg ccgagcgatg
     3661 gggcgaggag acctacgagg agcgggcccg gcaggtcaag gccgcgttca aggccctcca
     3721 gaaggagatc atgcggaagc gcgtgatcga ggaggggatc cggatcgacg ggcggaaggc
     3781 cgacgagatc cgccccatcc actgcgcggt cgatctcgtc ccgagggcgc acggctccgg
     3841 tctgttcgag cggggcgaga cccaggtcct gaacgtggcc acgctgggca tgctgcgcat
     3901 gacgcagatg atcgacacgc tcgaccccga ggaggagaag cggtacatgc accactacaa
     3961 cttcccgccc ttctccacgg gggagcccgg gttcatgcgg gggccgaagc gccgcgagat
     4021 cgggcacggc gcgctggccg agcgagcgct cgcgtccgtg atcccgaacg aggaggagtt
     4081 cccgtacgcc ctgcggctcg tctccgacgt gctggcgtcg aacggctcca cctcgatggc
     4141 ctcggcgtgc gcctcgacgc tctcgctgat ggacgcgggg gtcccgatca aggccccggt
     4201 ggccgggatc gcgatggggc tgatcgcaga gggcgatcgc ttcgtgacgc tgaccgacat
     4261 cctgggggcc gaggacgcgc tgggggacat ggacttcaag gtggccggga cgaaggactt
     4321 catcacggcc ctgcagctcg acacgaaggt gaccggcctg ccggccgagg tcctcagcgg
     4381 agccctgaag caggctcgcg aagcgaggct cttcatcctc gaccggatgc tggaggcgat
     4441 ccccgagccg cggtccgcgc tgtctgccaa ggcgccgcgc gtggaggcga tccagatccc
     4501 cgtggacaag atcggcgagg tgatcggacc gaaggggaag cggatcaacg agatcatcgc
     4561 cgagaccggg gccgacgtgg acatccacga cgacggcacg gtcttcatcg gctccaagga
     4621 gggcgagggg gccgagcggg ccaaggagat gatcaacgcg atcgtcaac
//
LOCUS       DASUTZ010000210        10665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_72576, whole genome shotgun sequence.
ACCESSION   DASUTZ010000210 DASUTZ010000000
VERSION     DASUTZ010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10665
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72576"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1302
                     /locus_tag="VF097_09120"
     CDS             <1..1302
                     /locus_tag="VF097_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012891389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX6262993.1"
                     /translation="DAVLPLCRTASGAPHALARMSERGLRVIAVAARDLEGGPAPATA
                     EEAERELDLLALIGLEDPPRPEAAEAIAACRRAGIRVAMVTGDHPTTATAIADETGLR
                     SPGAPVLVGSDLPPDDEALGELVDRDGVVIGRVDPEDKLRIARALRKRGHIVAMTGDG
                     VNDGPALHEADIGVAMGRSGTDVAREAADLVLLDDDFATIVTAVEEGRAIFANARRFL
                     TYHLTDNVAELTPFLVWALSGGSFPLALGVLQILALDLGTDTLSAVALGAEPAEPRTL
                     EQLPVRGRLLDRGVARRAFGVLGPVEGLMGMAAFLVSLLAWGWRPGDPFPTGAPLLAA
                     SGATFMAVVIGQTANAFACRSATRWPGALGWLTNRLLVVGAAIELLLSLGFLLIPPVA
                     ELLRHAAPALAGWGMALLTGPAVLAADALHKAAGRRARHDG"
     gene            1327..1704
                     /locus_tag="VF097_09125"
     CDS             1327..1704
                     /locus_tag="VF097_09125"
                     /inference="COORDINATES: protein motif:HMM:NF025601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HEX6262994.1"
                     /translation="MAEDRVWTEKVKVQADALREKVEELLREGNVRRIVVKNSEGQTV
                     IELPLTFGIVGAVAAPMITAVGAMAGVAARWSLEIERREPAGKKATPAKSTAKKSTAK
                     KASAKKAAAKKTSAKKGTSKKAE"
     gene            1782..2561
                     /gene="xth"
                     /locus_tag="VF097_09130"
     CDS             1782..2561
                     /gene="xth"
                     /locus_tag="VF097_09130"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019587983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX6262995.1"
                     /translation="MRIVSWNVNSIRMRLPRTEALLRRHEPAVLCLQELKSAEETFPS
                     DAFEALGYHAAVHAQAGRNGAAILSRQPLEDVTRGFPGDPAPEQARVVSATVAGIRVI
                     DLYVVNGVAVGAPQYELKLKWLDALSSWLRIEHDPTDPLLVIGDFNVAPEDLDVHDPE
                     RWRGRNLASEPERERVRELLGWGLVDLMRLHEGGPGPFTFWDYRAGAFHRGWGLRLDL
                     ALATQPIAERCERVWVERDERKPTFGEGKPSDHAPLIVELG"
     gene            2730..3983
                     /locus_tag="VF097_09135"
     CDS             2730..3983
                     /locus_tag="VF097_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011370488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX6262996.1"
                     /translation="MRFNAAWSMAVGGMIGGGIFSVLGVVTSVAGPLFPLSFVMGGLI
                     ALATAWSYAALARAMGEGGGAFTFLRHERLPRAAAALSWVLILGYTLTMAVYAFTFSA
                     YLSEGLGLGRGLTSAVAIAIVATLVGINLAGVRQAASVEIVSVYAKVLILLTLGIVGV
                     VHWDVGALSRGVSTSGGLIGAVVGASAVFMAYEGFQLLTYDYDDIEDPDRNLTRVLLT
                     AVGAVILVYVVVSLGAVMLTGAGTIVQHKEVALAIAGGRAAGSAGKWTVTVAAALSTA
                     SAINATLFATARLTRRVADAGELPRILGARNRRGMPHTAVILLGGIAAALAVLAGLSR
                     LVEAASLTFLLTFAMVAAIAWKELPMGRWVSGAGALGSLGAACVLAVRMALNDPWTLA
                     ALAVVTASAVLLRPLVVRRSGSATS"
     gene            complement(3998..4348)
                     /locus_tag="VF097_09140"
     CDS             complement(3998..4348)
                     /locus_tag="VF097_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012694571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEX6262997.1"
                     /translation="MAKLLINVTHGPEHPTRAALGFLVGSAAVEAGHEVSLFLAGDAV
                     QLLREPVLDHLVGLGTGSLRESYDKLREGGARFYVSGMSSKARGFEPDGIAEAAMPGK
                     LVELALEADRVFTY"
     gene            complement(4384..4470)
                     /locus_tag="VF097_09145"
     tRNA            complement(4384..4470)
                     /locus_tag="VF097_09145"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4434..4436),aa:Ser,seq:tga)
     gene            4523..4795
                     /locus_tag="VF097_09150"
     CDS             4523..4795
                     /locus_tag="VF097_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262998.1"
                     /translation="MDARYRWFVGLSEPESLAEVVDLLGRRRGFMLAHWGAEDIAGHE
                     AGMKVEVGDRAYVEMLRESDGSPHRRMDPILERAVRARPLSLLDLV"
     gene            complement(4792..6063)
                     /locus_tag="VF097_09155"
     CDS             complement(4792..6063)
                     /locus_tag="VF097_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6262999.1"
                     /translation="MDGDGLASSVTRRGALAALIAVALLVPPTAALAAPRDLRPYRGL
                     GSWVDIFDHRDLRDPVGTVEAMHVRGVRTLYLETSNFSQPRALVHRRATARFLHAAHA
                     RGMKVVAWYLPGFQNLRRDRLRSLAAIRFRTAKGHRFDSFALDIESSLVDPPDRRTRR
                     LLRLSRQLRKKARPSYTLGAIIPAPRGIQLATGYWPRFPYRRLARIYDVFVPMGYYTY
                     RYEGREAAHEYTRRNVQILRRRTGDPTVPIHMIGGVSGESSRGEVRGFVRAVREHGLL
                     GGSLYDFRGTRARHWEELAAVPVNPRQDVPLPAPLSHAGPLGNVPGVDVQHPKEVTFK
                     VGRRAGSWDLTYEAFDAGPGEVEILVNWKRLGEVVATPADAWGPPVTVTIPDGLLRDE
                     ARNLIHFVAPGDHPSWSTWGVRGVSLSAAPA"
     gene            complement(6033..7070)
                     /locus_tag="VF097_09160"
     CDS             complement(6033..7070)
                     /locus_tag="VF097_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020518204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6263000.1"
                     /translation="MEHRVLGRTGVRVSAYCLGTMMFGSWGNPDADECVKMIHRAMDA
                     GINFIDTADVYSKGESEEIVGKALSVRRDHVVLATKVHGRMGQKPNQGGNSRLWIMRE
                     VERSLRRLGTDYLDLYQMHRPDPSVDLEETLGALTDLVRQGKVRYAGCSAYPAWQIVE
                     SHWVSERRGLERFVTEQPPYSIFVRGIERDVLPVTRRYGMGVLVYSPLNGGWLAGRYR
                     KGRENPADSRAELMKERGRFRERYDREREPVRRKLDLVERLEEVAEGAGLSLARMAMA
                     FVLAHPDVTSAIVGPRTPEQLEELLEGADTRLTSDVLDAIDEVVPPGTLVDDADQGWE
                     GPWMETASRRR"
     gene            complement(7081..8349)
                     /gene="serS"
                     /locus_tag="VF097_09165"
     CDS             complement(7081..8349)
                     /gene="serS"
                     /locus_tag="VF097_09165"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014910424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX6263001.1"
                     /translation="MLDLRALRDDPEPVKAALARRDPELPAEVDRLLALDEERRRLTV
                     RADELRAEQNRASKAIGAAEEEERDRLIEEVRRVSVELKEVEPRLVEVDEGIGALLAR
                     LPNPPHESVPDGETDEDNEVISVVGEPADFGFDPKDHVDLGTALGLLDLERAARTSGS
                     RFVYLTGDAVLLEWALVRYCLDALMPEGFVPMIPPVLVREEAMYGTGFLPTDEAQLYM
                     TRDDDLYLVGTSEVPLAAFHQGDILEDLPRRYVGYSTCFRREAGAHGKDTRGMFRVHQ
                     FDKVEMFTFVPPDASQEEHERLLALEERIVSGLGLPYRVVDVCIGELGASAARKYDIE
                     VWLPGQGRYRELTSCSNTTDYQARRLEVRMRGPRGNVPVHTLNGTACAVGRTLIAILE
                     NHQREDGSVAVPQVLHPYLPEGLRVLGPPG"
     gene            complement(8354..8830)
                     /locus_tag="VF097_09170"
     CDS             complement(8354..8830)
                     /locus_tag="VF097_09170"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HEX6263002.1"
                     /translation="MAFSSELLTILVIVALGLAVLSLFLLATTAAPRRRLRGAAAPPS
                     ATLEGQADAIRRLQVSVEELSQADARLREHLAASVQRVGLIRYDAFEDMGGHLSFSLA
                     LLDEAGDGVVVSAINGRQDTRVYAKPVRGGDSGHNLSDEEAQAIRQAMAGSPAVSA"
     gene            9278..10621
                     /locus_tag="VF097_09175"
     CDS             9278..10621
                     /locus_tag="VF097_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HEX6263003.1"
                     /translation="MRKRIFLNLVAVAAVATALSASPLLAGDQEEAVQEFGSRFDAVE
                     ANEAALNANAPSSFAPCVNGTAGEFPCDNVDMLSFVPHGALGTTFVNDIWGWTDPATG
                     KDYALVGASEGTVFVDISDPKRPQVLGILPTHSTAGGDFWRDIKVYEDHAFIVSENSG
                     HGMQVFDLTQLRDWDGTYTTYSNTAHYDVFGRAHNININEDTGFAYAVGARQGEGCDF
                     GLHMIDISDPTNPTFAGCFGDNGYVHDTQCVIYEGPDAEHRGKEICFNSNAPGAYGST
                     NHWVDIVNVTDKANPVSLGRSDYPMSGYSHQGWLTPDQATFLHGDEGDEFIHGLGTTT
                     RVWDVSDLDDPELVGVHDNGTTSIDHNVYTEGRLAFASNYTTGLRVWDTRDADSGQLT
                     EIGFFDVYPENDNASFEGGTWSNYTYFSQKKVVAVSSIDRGLFILRPRGGLNNAP"
BASE COUNT         1589 a         3704 c         3813 g         1559 t
ORIGIN      
        1 gacgcggtcc tgcccctgtg caggaccgcg tccggggccc cccacgccct ggcccgcatg
       61 agcgagcgag gcctccgcgt gatcgccgtg gccgcccggg acctcgaggg cggccctgcc
      121 ccggccacgg cggaggaggc cgagcgcgag ctggacctcc tcgccctgat cggcctggag
      181 gatccaccgc gtccggaggc cgcggaggcg atcgcggcct gccggcgtgc cgggatccgc
      241 gtggcgatgg ttaccggcga ccaccccacg accgcgacgg cgatcgcgga tgagacgggg
      301 ctgcgctcgc ccggcgctcc cgtgctcgtg ggctcggacc tgccgccgga cgacgaggcc
      361 cttggggagc tcgtcgatcg ggacggcgtc gtgatcgggc gcgtggatcc cgaggacaag
      421 ctgcgcatcg cccgcgcgct gaggaagcgg gggcacatcg tggcgatgac cggcgacggc
      481 gtcaacgacg gaccggccct ccacgaggcc gacatcgggg tggccatggg tcggtcgggg
      541 accgacgtag cccgggaggc ggccgatctc gttctcctcg acgacgactt cgccacgatc
      601 gtcacggcgg tcgaggaggg gcgggccatc ttcgccaacg cccggcggtt cctcacctac
      661 cacctcaccg acaacgtggc ggagctcacg ccgttcctgg tctgggccct ctcgggcggg
      721 agcttccccc tcgcactcgg cgtcctgcaa atcctcgccc tcgacctggg caccgacacg
      781 ctctcggcag tggcactggg cgcggagccc gcggagccga ggacgctcga gcagctgccc
      841 gtcagggggc gcctcctgga ccggggcgtg gccaggcgcg cattcggcgt cctgggcccg
      901 gtcgagggcc tgatggggat ggccgccttc ctcgtctcgc tcctggcgtg gggctggcgg
      961 ccgggcgacc ctttccccac gggggctccc ctgctggcgg cctccggggc caccttcatg
     1021 gcggtagtca tcgggcagac ggccaacgcc ttcgcctgtc gcagcgcgac ccggtggccc
     1081 ggggcgctgg gctggctcac gaatcgcctc ctggttgtgg gagccgcgat cgagctgctg
     1141 ctctcgctgg ggttcttgct gatcccgccc gtggccgagc tcctgcggca cgcggccccg
     1201 gccctcgccg gctgggggat ggcgctcctg accggccccg cggtgctggc ggccgacgcc
     1261 ctgcacaagg cggccggccg ccgggcgagg catgatgggt aggcacgcga tggagaggag
     1321 accgcgatgg cagaggacag ggtctggacg gagaaggtga aggtgcaggc cgacgccctt
     1381 cgggagaagg tggaggagct cctccgcgag gggaacgtcc gccgcatcgt cgtgaagaac
     1441 tcggagggcc agacggtgat agagctgccg ctgaccttcg ggatcgtggg agccgtggcg
     1501 gcgccgatga tcacggccgt gggcgcgatg gctggcgtgg ccgcccgctg gagcctcgag
     1561 atcgagcgcc gcgagcccgc cgggaagaag gccaccccgg cgaagagcac ggccaagaag
     1621 agcacggcca agaaggcttc cgccaagaag gccgcagcga agaagacgtc ggcgaagaag
     1681 ggcacctcga agaaggccga gtaggctccc cgagccatga gcctctggca gtggctgctc
     1741 ctgttcgcgg cgggagcggt gatgttgacc tgggtcacct catgaggatc gtcagctgga
     1801 acgtcaactc gatcaggatg cggctgcccc gcaccgaggc cctgctgcga cgtcacgagc
     1861 ccgccgtgct gtgtctccag gagttgaagt ccgccgagga gacgttcccc agcgatgcct
     1921 tcgaggcgct cggctaccat gcggcggtcc atgcccaggc cgggcgcaac ggggccgcca
     1981 tcctctccag gcagccgctc gaggacgtga cacgagggtt ccccggcgat cccgcgcccg
     2041 agcaggccag ggtcgtctcc gccacggtgg ccgggatccg cgtgatcgac ctgtacgtgg
     2101 tgaacggggt cgccgtcggc gcgccacagt acgagctcaa gctgaagtgg ctcgacgccc
     2161 tctcctcatg gctccggatc gaacacgatc caaccgaccc cctcctcgtg atcggggact
     2221 tcaacgtggc gcccgaggac ctcgacgtgc acgaccccga gcgctggcgc ggtcggaacc
     2281 tggccagcga gccagagcgg gagcgggtgc gggagctcct ggggtgggga ctcgtggacc
     2341 tgatgcggct ccatgagggg gggcccggcc cgttcacctt ctgggactac cgcgccgggg
     2401 cgtttcaccg gggctggggg ctccggctcg atctggcgct cgccacgcag ccgatcgccg
     2461 agcgatgcga gcgggtctgg gtggagcggg acgagcggaa accgaccttc ggtgagggga
     2521 agccgagcga ccacgccccc ctgatcgtcg agctcggttg acccgggttc aggaggacag
     2581 ccggtccccg ctcaggcccc cagagtcctc gtcgtccacc tcgacctcag ggagtccgtg
     2641 accgccggtg tggggtaagg agccagcggg atggagatgg cacatcgcgt caaggacgga
     2701 tcggaggcgg caccataggc gacggacgca tgcgattcaa cgccgcatgg tcgatggcgg
     2761 tgggcggcat gatcggcggc ggcatcttca gcgtcttggg ggtggtcacg tcggtggcgg
     2821 ggccgctctt ccccctgagc ttcgtgatgg gcggcttgat cgccctcgcc acggcctggt
     2881 cctatgcggc gctcgcgcgc gccatgggcg agggcggagg cgcgttcacc ttcctgcgcc
     2941 acgagcgcct ccctcgtgcc gcagctgcgc tctcttgggt gctgatcctc ggatacacgc
     3001 tgaccatggc cgtctacgcc ttcacgttca gcgcctacct ctccgaaggt ctggggctgg
     3061 gcagagggct cacctcggcg gtggcgatcg cgatcgtcgc caccttggtg gggatcaacc
     3121 tcgcgggcgt gcgacaggcg gcctccgtcg agatcgtcag cgtgtacgcc aaggtgctga
     3181 tcctgctgac gctcggcatc gtgggagtcg tccactggga cgtgggcgcg ctctcacgcg
     3241 gcgtgtcgac gtccggaggg ctcatcggtg ccgtcgtcgg ggcctccgcc gtcttcatgg
     3301 cctatgaggg cttccagctg ctcacctacg actacgacga catcgaggac cccgatcgga
     3361 acctgaccag agtactgctc accgccgtgg gagcggtgat cctcgtgtac gtggtcgtct
     3421 ccctcggcgc cgtgatgctc acgggggccg gcaccatcgt ccagcataag gaagttgcgc
     3481 tcgccatcgc gggcgggcgg gcggcaggct ccgcgggcaa gtggacggtc accgtcgccg
     3541 ccgccctttc cacggcctcg gcgatcaacg cgacgctgtt cgccaccgcg cgactgactc
     3601 ggcgggtcgc cgacgctgga gagctcccac gcatcctggg ggcacggaac cgacgaggca
     3661 tgccgcacac ggcggtgatc ctcctcggcg ggatcgccgc ggcgctcgcc gtcctcgcgg
     3721 gactctcgcg gctcgtcgaa gcggcgagcc tgaccttcct cctgacgttc gcgatggtgg
     3781 cagccatcgc gtggaaggag ctgccgatgg gccggtgggt ctcgggcgcg ggtgcgctcg
     3841 gatcactggg agcggcctgc gtcctagcgg tccgtatggc gctcaacgac ccatggaccc
     3901 tggctgcgct cgccgtcgtc acggcctccg cggtgctcct ccgaccgctc gtcgtcaggc
     3961 gtagcggctc ggcgacctcg tgaccgcttc tcgctcctca gtaggtgaag acgcggtccg
     4021 cctcgagagc cagctcgacg agcttgccgg gcatcgccgc ctccgcgatc ccgtccggct
     4081 cgaagccgcg ggccttgctc gacatacccg agacgtagaa ccgagcgccc ccctcgcgaa
     4141 gcttgtcgta ggactcccgg aggctgccgg ttcccagccc gaccaggtgg tcgaggacgg
     4201 gctcgcggag gagctggacc gcgtcgccgg ccaggaagag cgagacctcg tgcccggcct
     4261 cgaccgccgc gctccccacc aggaagccga gcgccgctcg ggtagggtgc tccgggccgt
     4321 gggtcacgtt gatcaggagc ttcgccatgc gagcatcata gggaatgtgg tggatccggt
     4381 gagcgggggg actgggattc gaacccaggg gcccctcgcg ggaccaacgg ttttcaaggc
     4441 cgtcccgttc gtccgctccg gcatcccccc aaggcagtgt agccgcaccc cgggcggcct
     4501 cctcaccccc catactggaa cgatggacgc ccggtatcgg tggttcgtgg gcctcagcga
     4561 gcccgagtcg ctggccgagg tcgtggatct cctgggccgc cgacgcgggt tcatgctcgc
     4621 ccactgggga gcagaagaca tcgccgggca cgaggccggg atgaaggtcg aggtcggcga
     4681 ccgcgcctac gtcgagatgc tccgcgagag cgacgggagc cctcaccggc ggatggatcc
     4741 gatcctggag cgggcggtcc gcgcccggcc gctctcgctc ctggacctgg tctaggcggg
     4801 cgccgcggag agcgagacgc cccgcacccc ccacgtgctc cacgacggat ggtcgccggg
     4861 cgcaacgaag tggatcaggt tgcgggcttc gtcccggagg aggccgtccg ggatcgtgac
     4921 cgtcaccggc gggccccacg cgtcggccgg agtggcgacc acctcgccca gccgcttcca
     4981 gttgacgagg atctccacct cgccggggcc ggcgtcgaag gcctcgtacg tgaggtccca
     5041 cgaccccgcc cgcctcccca ccttgaaggt gacctccttc ggatgctgga cgtcgacgcc
     5101 ggggacgttc ccgagagggc ctgcgtggga gagcggcgcg ggaagtggga cgtcctgccg
     5161 cgggttgacc gggacggccg ccagctcctc ccagtgccgc gctcgggttc cgcggaagtc
     5221 gtagaggctc cccccgagca gcccgtgctc tcggacggcc cggacgaagc cgcggacctc
     5281 gccgcggctc gactcgccgg agacgcctcc gatcatgtgg atcgggaccg tcggatcccc
     5341 cgtcctccga cggaggatct ggacgttgcg cctcgtgtac tcgtgggcgg cctctcgacc
     5401 ctcgtaccga tacgtgtagt agcccatcgg gacgaacacg tcgtagatgc gcgccagccg
     5461 ccggtagggg aagcggggcc agtagcccgt cgccagctgg atcccgcggg gagccgggat
     5521 gatcgcgccc agcgtgtagc tggggcgcgc cttcttccgc agctgtcgcg agaggcgaag
     5581 cagccgcctg gtgcggcggt cgggcgggtc gaccagggac gactcgatgt ccagggcgaa
     5641 ggaatcgaag cggtgtccct tcgccgtgcg gaagcggatc gcggccagcg accgcagccg
     5701 gtccctccgg aggttctgga agcccgggag gtaccaggcc acgaccttca tgcccctggc
     5761 gtgggccgcg tggaggaatc gggcggtcgc cctgcggtgg acgagggctc ggggctgcga
     5821 gaagttcgac gtctcgaggt acagcgtgcg gacgccgcgc acgtgcatcg cctccaccgt
     5881 acccacgggg tcgcggagat cccgatgatc gaagatgtcg acccaggagc cgagcccccg
     5941 gtagggcctg aggtcccggg gtgcggcgag cgcggcggtc ggcggcacga gcaaagcgac
     6001 tgcgatcagc gcggcgaggg ctccgcgccg cgtcaccgac gacgcgaggc cgtctccatc
     6061 cacggcccct cccagccttg gtcggcgtcg tccacgaggg tgcccggggg cacgacctcg
     6121 tcgatcgcat cgagcacgtc ggacgtgagc ctcgtgtcgg cgccctccaa gagctcctcg
     6181 agctgctcgg gagtgcgggg cccgacgatg gccgaggtca cgtcggggtg cgccagcacg
     6241 aaggccatcg ccatccgggc cagggagagc ccggcgccct ccgcgacctc ctcgagccgc
     6301 tccaccaggt cgagcttccg ccggaccggc tcccgctcgc ggtcgtaccg ctcgcggaag
     6361 cggccgcgct ccttcatcag ctcggcccgg gagtcggccg ggttctcccg gcccttccgg
     6421 tacctgccgg ccagccaccc cccgttgagc gggctgtaga cgagaacacc catgccgtac
     6481 cgacgggtca ccggcaggac gtcgcgctcg atgccccgca cgaagatcga gtacggaggc
     6541 tgctcggtga cgaaccgctc caggccccgc cgctcgctga cccagtgcga ctcgacgatc
     6601 tgccaagcgg gatacgcgga gcaccccgcg taccggacct tcccctgacg gacgaggtcg
     6661 gtcagggcgc ccagcgtctc ctcgaggtcg acgctcgggt cgggccggtg catctgatag
     6721 agatcgaggt agtcggtccc caggcgacga aggctgcgct ccacctcccg catgatccac
     6781 agcctcgagt tgccgccctg gttcggcttc tggcccatcc ggccgtggac cttcgtggcc
     6841 agcacgacgt gatcgcgccg caccgagagc gccttcccca cgatctcctc ggactccccc
     6901 ttcgagtaga cgtcggccgt gtcgatgaag ttgatgcccg cgtccatcgc gcggtggatc
     6961 atcttcacgc actcgtcggc atcgggattg ccccacgagc cgaacatcat cgtgcccaga
     7021 cagtacgcgc tgacgcggac gccggtgcgg ccgaggaccc gatgctccat gccgaagagc
     7081 ctagccgggc gggccgagga cgcgaagacc ctcgggcaga tacgggtgga ggacctgggg
     7141 caccgcgacc gagccgtcct cgcgctggtg gttctcgagg atggcgatta gggtccggcc
     7201 gacggcgcac gcggtgccgt tgagcgtgtg gacgggcacg ttcccccgcg ggccgcgcat
     7261 ccggacctcg aggcgccggg cctgatagtc ggtggtgttc gagcacgagg tcagctcacg
     7321 gtagcggccc tggcccggca gccagacctc gatgtcgtac ttccgggccg ccgacgcgcc
     7381 cagctcaccg atgcagacgt cgaccacgcg gtacggaagg ccgaggccgc tcacgatccg
     7441 ctcctcgagc gccagcaggc gctcgtgctc ctcctgggag gcatcgggcg gcacgaacgt
     7501 gaacatctcc accttgtcga actggtggac gcggaacatc ccgcgggtgt ccttgccgtg
     7561 ggcgccggcc tcgcgtcgga agcacgtcga gtagcccacg taccgccgcg ggaggtcctc
     7621 gaggatgtcg ccctggtgga aggccgccag cgggacctcg gaggtgccca ccaggtacag
     7681 gtcgtcgtcg cgcgtcatgt agagctgagc ctcgtcggtg gggaggaacc cggtgccgta
     7741 catcgcctcc tcgcggacca gaacgggagg gatcatcggg acgaacccct cgggcatgag
     7801 cgcgtcgagg cagtagcgga cgagggccca ctcgagcagc accgcgtctc cggtcaggta
     7861 gacgaagcgc gagcccgagg tgcgggctgc ccgctccagg tcgaggaggc ccagggcggt
     7921 acccaggtcc acgtggtcct tcggatcgaa cccgaagtcg gccggctcgc cgacgaccga
     7981 gatcacctcg ttgtcctcgt cggtctcccc gtcgggcacg ctctcgtgtg gggggttggg
     8041 aagccgcgcc aggagggctc cgatcccctc gtcgacctcg accagccggg gctcgacctc
     8101 cttcagctcg accgagacgc ggcgcacctc ctcgatgagg cgatcccgct cctcctcctc
     8161 ggcggctccg atcgccttcg aggcccggtt ctgctcggcc ctcagctcgt cggcccggac
     8221 ggttaggcgg cggcgctcct cgtccagcgc gaggaggcgg tccacctcgg ccgggagctc
     8281 gggatcgcgt cgggccagcg cggccttgac tggctcgggg tcgtcgcgga gggcccgcag
     8341 gtccagcacg gggtcaggcg ctcacggccg gggatccggc catcgcctgg cggatggcct
     8401 gggcctcctc gtcggagagg ttgtgtcccg agtcgccgcc ccgcaccggc ttggcgtaga
     8461 cgcgggtgtc ctgccggccg ttgatcgcgc tcacaacgac gccgtcgccc gcctcgtcga
     8521 gcagggccag cgagaacgac aggtgccctc ccatgtcctc gaacgcgtcg tagcggatca
     8581 ggcccacccg ctgcaccgaa gcggcgaggt gctctcggag gcgggcgtcg gcctgcgaca
     8641 gctcctccac cgagacctgg aggcggcgga tcgcgtcggc ctggccctcg agggtggccg
     8701 agggtggcgc cgcggcgccg cgcaggcgac gccgtggcgc cgccgtcgtc gccagcagga
     8761 acagggagag gaccgccagc ccgagggcca cgatcacgag gatggtcagg agctcgctgg
     8821 agaaggccat ggatgcgggg agtatacgcc gggttcgcgc ccgcccgggc caccagactc
     8881 ccctcagcca cggccggccg cctgccgatg cctggaccac ggcgcctggc gggtctggga
     8941 gcgtgcggtc tggaactcgc ctcggcctcc tctcggggcg agcccgcccg tcgctcctcg
     9001 atttgagact catccccggc cttcgctagg gtcaagagac ccgaggcaag gatccgacgc
     9061 gaagcttgat caacgtgctt ccgagcgctc atcgcgacga ggacgtcgca cacccgggca
     9121 tcgccatgcc ccgatccggg gcggcgttcc tgcgagagga acctctgccg tcgaaggggg
     9181 gtggtagacg aggatcgaca acccgccgtt gatcccacca agacatcccc ggcgcatcgt
     9241 gcgctgggac gttggtggcc cgcaaaggag gaagagcatg aggaagcgga tcttcttgaa
     9301 tctggtggcg gtagccgccg tcgcaaccgc cctctccgcg tcgccgctgc tcgcgggcga
     9361 tcaggaagag gcggtgcaag agttcggctc ccgcttcgat gcggtggagg cgaacgaggc
     9421 ggcgctgaac gcgaacgcgc ccagcagctt cgcgccatgc gtcaacggca cggccgggga
     9481 gttcccctgc gacaacgtcg acatgctgag cttcgtgccg cacggggcgc tcggcacgac
     9541 gttcgtcaac gatatctggg gctggaccga ccctgcgacc ggcaaggact acgcgctggt
     9601 cggggcgagc gaggggaccg tgttcgtcga catcagcgac cccaagcggc cccaggtgct
     9661 gggcatcctg cctacgcatt ccaccgcggg tggggacttc tggcgcgaca tcaaggtgta
     9721 cgaggaccac gccttcatcg tctcggagaa cagcggccac gggatgcagg tcttcgacct
     9781 gacccagctc cgcgactggg acgggacgta caccacgtac tcgaacacgg cgcactacga
     9841 cgtgttcggg cgcgcgcaca acatcaacat caacgaggac accgggttcg cctacgctgt
     9901 gggcgcacgt caaggtgagg ggtgcgactt cggtctccac atgatcgaca tcagcgaccc
     9961 gacgaacccg acgttcgcgg gatgcttcgg cgacaacggc tacgtccacg acacgcagtg
    10021 cgtgatctac gagggcccgg atgccgaaca ccgcggcaag gagatctgtt tcaactccaa
    10081 cgcccccggt gcgtacggca gcacgaacca ctgggtcgac atcgtgaacg tgaccgacaa
    10141 ggcgaacccc gtctcgctgg ggaggtccga ctacccgatg tccggctact cgcaccaggg
    10201 ctggctgacg ccggaccagg cgaccttcct tcacggggac gagggcgatg agttcatcca
    10261 cggcctcggc acgaccacgc gggtgtggga cgtctccgac ctcgacgacc cggagctggt
    10321 cggcgtgcac gacaacggca cgacgtcgat cgaccacaac gtgtacaccg agggccgcct
    10381 ggccttcgcg tccaactaca cgaccggtct ccgggtgtgg gacacccggg acgccgactc
    10441 cggtcagctc accgagatcg ggttcttcga cgtctacccg gagaacgaca acgcctcctt
    10501 cgagggtggt acgtggagca actacacgta cttctcccag aagaaggtcg tggccgtcag
    10561 cagcatcgac cgcgggctgt tcatcctgcg gccgaggggc ggtctgaaca acgctccctg
    10621 agcgggagta gacgggaagg gtgggggccg cgcgcgcgag aagcg
//
LOCUS       DASUTZ010000211         4121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_461657, whole genome shotgun sequence.
ACCESSION   DASUTZ010000211 DASUTZ010000000
VERSION     DASUTZ010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461657"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1554)
                     /locus_tag="VF097_09180"
     CDS             complement(<1..1554)
                     /locus_tag="VF097_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6263004.1"
                     /translation="MAPSEEVGSLHLTHGARRMAERALELQRKRHHHQLGVNHWLLAL
                     LERHGAMAEALVDGLDAELVRRQVRRRLDEGDAGGALELSDTVSRALQASGQEEASER
                     YLASVVLAAAGYSVRAESAQGAAAARQAQAATRPGPPTPMLDEFGVDLTRQAREGRLP
                     PVVGRESEIRLVCTTLCRRTKRNPALIGPAGTGKTAIVEGLAQRVVAGQVPDPLKGIR
                     IVAIQPSSLVAGAGIAGQFEERVKAVLKEAEGPGIVLFIDELHSIVGSGGLMGATDVG
                     AQLKPALARGVSCIGATTDDEYRRFIERDRAMERRFNPIAVEEMARDDTLMVLEAHRD
                     VLGEMRGVEVPDDVLHWLLDFGEDFLRNRAFPDKAVDLLEQCVATAVMNGESKVDVPA
                     ARAVAEEMVGVPVDLEERVSELGRRLTEAALLKEQGVDELVARLSVTMRGLDLKPTRP
                     NAVILLADQAAETSEELAETMAEALLGDAKRVVELDFGQFDRAEDVNTLLGAPPAYIG
                     FGEHLPLHDL"
     gene            1733..2182
                     /locus_tag="VF097_09185"
     CDS             1733..2182
                     /locus_tag="VF097_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263005.1"
                     /translation="MDARVVEKTRDKVRKFLQRSFGGAFKEQGDIFWGRQGTTAVMIR
                     VLPWPSVEDAQVQVIAGIARDVDPTIECLSFLLKENQDFILGGFALEGDRDIIFRHGI
                     IGSKMDQKELEASVLAVASTGDKYDEIITSRFGGVRISDLAGEAAAS"
     gene            complement(2292..>4121)
                     /locus_tag="VF097_09190"
     CDS             complement(2292..>4121)
                     /locus_tag="VF097_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013734229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX6263006.1"
                     /translation="FEQVNDGTGDEDDACEPLVGFTAGNIAIAERGACNFTQKTLNAQ
                     AAGSIALVIYNNVGGSPFSPGGSAPGITIPTVMVSQDDGNTILAGLPATGTLIPSEGI
                     SNNQRVGVDSIAWGHEGGNDLTIASVDPGAADSPLSVDVSGDDITVNLGTDAGGALSS
                     TAADVVAAINGDSEASALVFAYTYRGSSGSGIAQPAPRRNLSDFLSAPASVPRDPRTV
                     KLLRIGKHRDGSRPGVFAYAQEHAREWVPPLVAIETAERLLRNYEDHGPTKQLVNRLD
                     IFILPSTNPDGGEYSFYDDNGQRRNMTRHCDVDGAYDLNARDNWGVDNNRNYDEYSLF
                     DGYSGASTNCQSDVFAGPAELSEPESANIDWVADEFENIKFSMNLHSSGNYFMWSPGS
                     YAMPGRVSAPRPSLAEESFFWGASSRILTEIKQHRGLSVTPARTGPISDVLYSAAGNS
                     GDMLWYKYGIYAWNFEVGTSFQPPFENPNPNGGSAHAESQEFANGLMELMRVAHDFDR
                     DHERPESWIEVTPSATPGMVNVVFETTEPAAVFYETDGSTPTYDSTLYASGGIREAGE
                     LLTVAEGTVFHFFSVDAAGNVENNYIPDGTRKNFNKATAVIGG"
BASE COUNT          589 a         1448 c         1405 g          679 t
ORIGIN      
        1 caggtcgtgc agcgggaggt gctcgccgaa cccgatgtag gcgggcgggg cgccaaggag
       61 cgtgttcacg tcctcggctc ggtcgaattg cccgaagtcc aactcaacga cgcgcttcgc
      121 gtcgccgagg agggcctccg ccatcgtctc cgcgagctcc tcgctggtct cggccgcttg
      181 gtcggccagg aggatcaccg cgttgggccg ggtgggcttg aggtcgagcc ctcgcatcgt
      241 caccgagagc ctggcgacca gctcgtccac gccctgctct ttcaggagag ccgcttcggt
      301 gaggcgccgt ccgagctcgg acacccgctc ctcgaggtcc accggcaccc ccaccatctc
      361 ctcggccacc gcgcgggcgg cggggacgtc gaccttggac tcgccgttca tcacggcggt
      421 ggcgacacac tgctcgagga ggtccaccgc cttgtcgggg aaggctcggt tgcggaggaa
      481 gtcctccccg aagtcgagca gccagtggag gacgtcgtcg ggcacctcca cgccccgcat
      541 ctcccccagc acgtcacggt gggcctccaa caccatcagg gtgtcgtcgc gggccatctc
      601 ctccaccgcg atcgggttga agcggcgctc catggcccgg tcccgctcga tgaaccgtcg
      661 gtactcgtcg tcggtggtgg cgccgatgca cgagaccccc cgagccagcg ccggcttgag
      721 ctgggccccc acgtcggtgg cccccatcag cccgccgctc cctacgatcg aatgcagctc
      781 gtcgatgaag aggacgatgc ccggcccctc ggcctccttg agcaccgcct tcacccgctc
      841 ctcgaactgc ccggcgatgc cggcccccgc cacgaggctc gacggctgga tggccacgat
      901 ccggatcccc ttgagagggt ccgggacctg gcccgccacc acgcgctggg ccagaccctc
      961 cacgatcgcg gtcttgccgg taccggccgg cccgatcagg gccgggttcc tcttcgtgcg
     1021 gcggcacagc gtcgtgcaca cgaggcggat ctcgctctcc cgcccaacga ccggcggcag
     1081 cctcccctcg cgggcctgcc gggtgaggtc gacaccgaac tcgtcgagca tcggggtggg
     1141 cgggccggga cgggtcgccg cctgagcctg cctcgcggcg gccgccccct gcgccgactc
     1201 cgctcgcacg ctgtacccgg ccgccgccag gacgacggag gcgaggtacc gctcgctcgc
     1261 ctcctcctgc ccggaggcct ggagggcgcg cgacacagtg tcgctcagct cgagggcgcc
     1321 tcccgcgtct ccctcgtcca gtcggcgacg gacctgccgc cggaccagct cggcatcgag
     1381 gccgtcgacg agcgcctcgg ccatggcgcc gtgacgctcc agcagggcga gcagccagtg
     1441 gttcacgccg agctggtggt ggtggcgctt ccgctggagc tcgagggctc gctcggccat
     1501 gcggcgggcc ccgtgggtga gatggagtga accgacctcc tctgagggtg ccatcggtcg
     1561 tcccccttcg cgttctctcc ccatacgaaa ccgcctggcg tgggagcgaa gtagggtcga
     1621 aggtcccggt ccgttcggtc ctctccggat ggggccgtcc ggccctacgg ccgatcggag
     1681 gccccgccta cgatgacgct gcacgcacca gccggtgcgg gggaggtgcg ccatggatgc
     1741 gcgggtggtc gagaagacca gggacaaggt gaggaagttc ctccagcgga gcttcggagg
     1801 ggctttcaag gagcaggggg acatcttctg ggggcggcag gggacgaccg cggtgatgat
     1861 ccgcgtgctc ccgtggccct cggtggagga cgcccaagtc caggtgatcg ccgggatcgc
     1921 gagggacgtc gatcccacga tcgagtgcct gagcttcctg ctcaaggaga accaggactt
     1981 catcctcggc gggttcgcac tcgagggtga ccgggacatc atcttccggc acggcatcat
     2041 cgggtcgaag atggatcaga aggagctcga ggcctcggtc ctggccgtgg cctccaccgg
     2101 ggacaagtac gacgagatca tcacgagccg attcggtggg gtgaggatca gcgacctggc
     2161 gggcgaggcg gcggcctcct gacggccggt cctccggaag ctcccgtgag tcgtggtgag
     2221 tagggccgaa ctcaaggcag tcgcccgcgc cccggcttca gcctccgcgc cccctcggcg
     2281 ctgcggcagg ctcagcctcc tatgacggcc gtcgccttgt tgaagttctt cctcgtaccg
     2341 tccgggatgt agttgttctc cacgttcccg gccgcatcga ccgagaagaa gtggaacacg
     2401 gtcccctccg cgacggtcag cagctcgcct gcctcgcgga tcccacccga ggcgtacagg
     2461 gtggagtcgt acgtcggcgt gcttccgtcc gtctcgtaga agaccgcggc gggctccgtc
     2521 gtctcgaaca cgacgttgac catgcccggc gtcgcgctgg gcgtcacctc gatccagctc
     2581 tccggccgct catggtccct gtcgaagtca tgggccaccc gcatgagctc catcagcccg
     2641 ttggcgaact cctgggactc cgcgtgcgcg gaccccccgt tggggttggg gttctcgaac
     2701 ggcggctgga acgaggtgcc cacctcgaag ttccaggcgt agatgccgta cttgtaccag
     2761 agcatgtccc cggagttccc cgccgccgag tacagcacgt cggagatcgg gcccgtcctg
     2821 gccggggtca ccgagagacc acggtgctgc ttgatctcgg tgaggatccg agaggaggct
     2881 ccccagaaga aggactcctc ggccaggcta ggccgcggcg ccgacacccg gcccggcatc
     2941 gcgtaggacc cgggcgacca catgaagtag ttgcccgacg agtggaggtt catcgagaac
     3001 ttgatgttct cgaactcgtc cgccacccag tcgatgttcg ccgactcggg ctccgacagc
     3061 tccgccggcc ccgcgaacac gtcgctctga cagttcgtgg acgcaccgct gtacccgtcg
     3121 aacaggctgt actcatcgta attgcggttg ttgtccactc cccagttgtc acgggcgttg
     3181 aggtcgtacg ccccgtccac gtcgcagtga cgggtcatgt tccgacgctg cccgttgtcg
     3241 tcgtagaagg agtactcgcc cccgtccggg ttcgtcgagg gcaggatgaa gatgtcgagc
     3301 cggttcacta gctgcttcgt cggaccgtgg tcctcgtagt tccggagcag ccgctcggcc
     3361 gtctcgatcg ccaccagcgg gggcacccac tcacgcgcgt gctcctgggc gtaggcgaac
     3421 accccgggcc tcgacccgtc gcggtgcttg ccgatccgca gcagcttcac cgtgcgcggg
     3481 tcgcgcggca ccgaggccgg cgcgctgagg aagtcgctca ggttccggcg cggcgcgggc
     3541 tgtgcgatcc cgcttccgct gctcccccgg taggtgtagg cgaacacgag cgcgctcgcc
     3601 tcagagtcgc cgttgatcgc ggccaccaca tcggcggccg tgctcgacag ggctccgccc
     3661 gcgtcggtcc caaggttgac ggtgatgtcg tcaccggaga catccacaga cagcggggag
     3721 tccgcagccc cggggtcgac gctggcgatc gtgaggtcgt tgccgccctc gtggccccag
     3781 gcgatcgagt ccacgccgac cctctggttg ttgctgatgc cctcgctcgg gatcagggtc
     3841 ccggtggcgg gcaggccggc gaggatggtg ttcccatcgt cctgagagac catcaccgtc
     3901 gggatcgtga tccccggcgc ggagccgccg ggagagaagg ggctgccgcc cacgttgttg
     3961 tagatcacga gcgcgatcga gccggcggcc tgggcgttca gggtcttctg cgtgaagttg
     4021 caggcgccgc gttcggcgat cgcgatgtta ccggccgtga agccgaccag cggctcgcag
     4081 gcgtcgtcct cgtccccggt cccgtcgttc acctgctcga a
//
LOCUS       DASUTZ010000212         9913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1368007, whole genome shotgun sequence.
ACCESSION   DASUTZ010000212 DASUTZ010000000
VERSION     DASUTZ010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9913
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368007"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3540)
                     /locus_tag="VF097_09195"
     CDS             complement(<1..3540)
                     /locus_tag="VF097_09195"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX6263007.1"
                     /translation="MRPSRALAIGITLSLTAGALLWAPPAASTQPQPPRASSRPAFVP
                     GELLIQFRPGTPAVERAAARARHGLTRVKGLPLPDLELVRFPGQGQPPEVAARVAADV
                     AIRAAEPNLLWYPDGIPDDPEFEWLWGLHNEGQTGGTPDADIDAPEGWAVAADSSDVV
                     VAVIDTGVWIDHPDLQAQIWTNPGESGGGKETNGVDDDMNGYVDDVHGWDFFNDDATV
                     FDANQPDDHGTHVAGTIAATIDNATHVAGVSQATIMPLKFLGPQGGATSAAIEALDYA
                     IDNGAHLTNNSWGGGGFSGMLQAKIEQANGAGVLFVAAAGNGGSDGEGDDNDATPHYP
                     ASYTNANVISVAATTDTDALAPFSNFGAVSVDLGAPGDDILSTVPRFAKAVLVGPDTG
                     AGVAYRSAFHTFGLEGITSGAHREAILSSALDWMGVSGTDPILLVDDDRGASFLTAYA
                     NALQSLGFTNRTVHTVPTAPPGCNNGPNAAEMSEYDAVIWFTGADFSCTLTGADQTQL
                     GTYLTGGGNLLLFGQDIGYDLIEDTGGSPPSTFLEAVLFADYVADIDFNFRPSGVAGT
                     PYASVPTVELSTSGSDSQLFSSALEPRPGAIAGMRGAPTAWFDGTSMATPHVTGAAAL
                     VMATHPSLGHLDVKARLLGRGDPLPGLAGKTVSGKRLNLASSLAFDPPGAPAALTVSA
                     SAGGQIDLSWTASPGPGVTGYNVYRGTTSGFPPSPGNRIASLVAGTSYSDPGSNTVDG
                     TTYYYLVRAENSQGDESGDSNEASATADHTPPEVAGTIPEDGETDVPVSTSVSVIFSE
                     AVEPSTVGASSITLTGPGGPVAGGVSLDPSGTEATLTPAANLLLESTYTADVTSDVED
                     LAGNPLTPASWTFDTTAVDTEAPDVEIVGPASGEGVRAQPHTMTGTATDDVEVDNVEV
                     AVRWDASGGTLYWDAGSADFVPGETWNQATIEPGVSSGGVTWSLDWMPPDADREPFAV
                     LARATDTSNNITDPPEEVSFWIDNVAPAGSVSIDDDAPLTGSRSVILTLTASDGSEIE
                     FSVANGEDPNGPWQPFAPSLSWSLSGGADGLRTVYVKFRDHAGNTTTVEDDILLDITA
                     PTASLGPLPAFQRTTRFDVSWTGGDAGSGLDGYRVEIRKAGLKGAWTPWTEWTTTSDE
                     VATFQGRTGRTYCFRVAATDQAGNTSSKDSECTA"
     gene            complement(3648..4106)
                     /locus_tag="VF097_09200"
     CDS             complement(3648..4106)
                     /locus_tag="VF097_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263008.1"
                     /translation="MTELRFTVGAPVTAERVLAALTDFSDRRPEIWPELDPDAYRVVE
                     LHATSALVREGQRKPRLWALEEYDWSTPGTVTWTARESNFCAPGSFMSARVEPVAGGG
                     SRMHVIWSRTGVGAKGKTIVGLMRLTRGRPLATSLSRALAGLRQATDVGG"
     gene            complement(4103..4408)
                     /locus_tag="VF097_09205"
     CDS             complement(4103..4408)
                     /locus_tag="VF097_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263009.1"
                     /translation="MLARVTVFKGSGENFDEASRNARENVAPALRELDGFAGMLVLAD
                     RGTGRSMAITLWETEEALRSSERAAEEMRAKTAATYDEEIVAVDTYDIVIDERGGDR"
     gene            complement(4484..6028)
                     /locus_tag="VF097_09210"
     CDS             complement(4484..6028)
                     /locus_tag="VF097_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015659022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX6263010.1"
                     /translation="MTDQLRLADLLVAISVATDIGMGQEPEKAARSCLLATRMARGLG
                     LPEAEVREVYLVTLLRHLGCTATAHEEAYLFGGDELASRPPSEAADFGEAREIVKLLL
                     ATGRGAGIRRPRLLARALMAGRKADAGILRAICEVGSHLASRLGLGTGVERALYQVME
                     RWDGTGAPEGASGEDIVLPARIAEVATQAVIFDRAGGPEAALAMAARRAGGWFDPAVA
                     EAFRRMGPQLLAEGAAEDPWEAVLAAEPEPVAVVGAAELDRVARTFADMVDLKSPYTL
                     GHSTGVAELAVEGGRSLGMSEDDLDELRLSALMHDVGRTGVSNGIWDKPGPLTTAEWE
                     MVRTHPYHTERILAHSAALAPLIPIAGAHHERQDGSGYHHGASGGEIPAAARILAAAD
                     AYQAMTQERPHRPARAPEEAAEAIAAAASEGRLDPDCARAVAEAGGHTGVRVKAERPA
                     GLSDREVEVLRLVARGLSNKEIAKRLFISPRTAEHHVQHIYAKIGSSTRASAAVFAME
                     HDLLGP"
     gene            complement(6042..7322)
                     /locus_tag="VF097_09215"
     CDS             complement(6042..7322)
                     /locus_tag="VF097_09215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELWxxDGT repeat protein"
                     /protein_id="HEX6263011.1"
                     /translation="MKDIKPGPGSSLNDEGIDFRRVGRWLLFSADDGVAGTELWRTDG
                     TRKGTRRVKDINPGPGSSVPAEVELMGGILFFQADDGATGWELWRTDGTKKGTRRVKD
                     INPGPGDSSPQGLTRLGKRILFTANDGSTGMELWRTDGTKKGTRRVKDINPGPSFAQP
                     HDFVRLGKKLLFSASNGTLSIELWRTDGTRKGTRLVKDIRPGPGDSSPGNLVRLGKRV
                     IFSATHDATGNELWRTDGTKKGTRLVKDIFLGGNSFPDRLTAYGKMILFRADGPGGRE
                     LWRTDGTKKGTRRVKDINPGPGDSEIDDVARFGKIMLFQANDGVAGTELWRTDGTKKG
                     TRRLKDINPGPGSSIPDKFTTVGNWSLFKADDGTTGPELWRTDGTKKGTRRLRDLNPG
                     PGAAYPGYFTPLGKWFLFGTYDGSSGYELWKLRR"
     gene            7525..8109
                     /locus_tag="VF097_09220"
     CDS             7525..8109
                     /locus_tag="VF097_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX6263012.1"
                     /translation="MDAIYFSGADEFRAWMEENHHTAAELYVGFHKKGAGIPSMTWEE
                     SVDVALCFGWIDGVRRSVDDERYVIRFTPRRPGSVWSARNIERAKALIEEGRMIPAGL
                     AAFEARREDRSPYSYERRASIRLDEDLERRFRRASKAWERFQAMAPSYRKSALHWVMS
                     AKRHETRERRLERLIADSAAGRRVPPLAPRERRG"
     gene            8269..8658
                     /locus_tag="VF097_09225"
     CDS             8269..8658
                     /locus_tag="VF097_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017277218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX6263013.1"
                     /translation="MGTATRERLDATFSALADPTRRAILTRLSQGEASVTQLAAPFDM
                     TLPAVSKHLKVLERAGLIARSRERQWRPARLAAEPLKEAADWLERYRRFWEESYDRLD
                     EYLDELQGRRKEEEDGEPAAGLDPHGP"
     gene            8618..9106
                     /locus_tag="VF097_09230"
     CDS             8618..9106
                     /locus_tag="VF097_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6263014.1"
                     /translation="MGNRLQVSTPTDREIVMTRVFDAPRDLVFEAHTSCEHLRNWWGP
                     RAMEISECEVDFWEGGAWRIVHRGPDGEEYGFSGEYREIVRPERITWTFEFDGAPGSV
                     SVETLTLEEHDGKTTVTATAVYDSKEARDAMIESGMETGAAETYERLDEYLEALRERA
                     PS"
     gene            9182..>9913
                     /locus_tag="VF097_09235"
     CDS             9182..>9913
                     /locus_tag="VF097_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013805615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6263015.1"
                     /translation="MIRTSGLVKRYGKVTALDGLDLEVPKGTVLGLLGPNGAGKTTAV
                     RILTTLLDADEGTAEVAGLDVRRQATEVRRVIGLSGQTAAVDEYLTGYENLEMVGRLY
                     HLGGPRSRARARELLEQFDLQEAGNRPAKTYSGGMRRRLDLAAALVAEPEVLFLDEPT
                     TGLDPRSRVQMWVTIQSLVHAGSTVLLTTQYMEEADRLADDIVVIDRGQKIAHGTADQ
                     LKLQVGGERLELVLGDMSDLESARSI"
BASE COUNT         1393 a         3436 c         3523 g         1561 t
ORIGIN      
        1 ggccgtgcac tcgctgtcct tgctcgaggt gttcccggcc tggtcggtcg ccgcgacccg
       61 gaagcagtac gtgcggccgg tcctcccctg gaaggtcgcc acctcgtcag atgtggtcgt
      121 ccactcggtc cacggcgtcc acgcgccctt cagcccagcc ttccggatct cgacgcgata
      181 gccgtcgagt ccgctccccg cgtccccgcc ggtccaggag acgtcgaatc tcgtggtccg
      241 ctggaaagcc ggcagcggac cgagggacgc cgtcggggcg gtgatgtcca agaggatgtc
      301 gtcctcgacg gtcgtagtgt tgccggcgtg atctcggaac ttcacgtaca ccgtccgaag
      361 gccgtcggcg cctccgctca ggctccacga cagcgagggg gcgaagggct gccacggccc
      421 gttcgggtcc tctccgttcg ccacgctgaa ctcgatctcg gacccgtcgg aggccgtcag
      481 ggtcaggatc accgaccttg agcctgtgag cggtgcgtcg tcgtcgatcg acacggatcc
      541 ggctggggcg acgttgtcga tccagaagga gacctcttct ggcgggtccg ttatgttgtt
      601 ggaggtatcc gtcgcccggg ccagcaccgc gaagggctcg cggtcggcat cgggaggcat
      661 ccagtcgagg gaccacgtca cgccccccga tgagaccccg ggctcgatgg tggcctggtt
      721 ccaggtctcg cccgggacga agtccgctga gcccgcatcc cagtagaggg tccccccgct
      781 cgcgtcccat cgcacggcca cctccacgtt gtcgacctcg acatcgtccg tcgccgtgcc
      841 ggtcatcgtg tgcggctggg cgcgaacgcc ctctccggac gcaggtccca cgatctcgac
      901 gtccggggcc tcggtgtcca ctgccgtcgt gtcgaaggtc cagctcgcag gggtgagcgg
      961 gttgccggcc aggtcctcga cgtcgcttgt cacatccgcg gtgtaggtcg actcgaggag
     1021 gaggttggcg gccggcgtca gcgtggcctc ggtcccggat ggatcgaggg agacgccgcc
     1081 cgcgacgggc cccccgggcc cggtcagggt gatcgaggac gctccgacgg tcgacggctc
     1141 caccgcctcg ctgaagatga cgctgacgct cgtggacacg gggacgtcgg tctcgccgtc
     1201 ctcggggatc gtgccggcga cctcaggggg cgtgtggtcg gccgtcgccg aggcctcgtt
     1261 ggagtcgccg ctctcgtccc cctgggagtt ctccgcgcgc accaggtagt agtaggtcgt
     1321 gccgtcgacg gtgttcgagc ccgggtccga gtacgacgtg ccggccacga gcgaagcgat
     1381 gcggttcccc ggcgagggag ggaacccgct ggtggtgccc cggtagacgt tgtagccggt
     1441 cacaccgggt ccgggcgatg ccgtccagga gaggtcgatc tgtcctccgg cggacgccga
     1501 gacggtgagc gccgccgggg cgcccggcgg gtcgaacgcg agggaggagg cgaggttgag
     1561 ccgcttcccc gagaccgtct tccccgccag ccccggcagg ggatcgcctc gtccgaggag
     1621 acgggccttc acgtcgagat ggccgagcga cgggtgcgtg gccatgacca aagccgccgc
     1681 ccctgtcacg tgcggtgtgg ccatcgaggt tccgtcgaac caggcggtgg gcgcgcccct
     1741 catgcctgcg atggctccgg gcctcggctc cagcgcgctg gagaagagct ggctgtccga
     1801 gccggacgtg ctgagctcga ccgtgggaac ggacgcgtat ggggtcccgg cgacacccga
     1861 cgggcggaag ttgaagtcga tgtcggccac gtagtcggcg aacagtaccg cctcgaggaa
     1921 ggtgctcggc ggcgaacctc ccgtgtcctc gatcaggtcg tacccgatgt cttgaccgaa
     1981 gagcaggagg ttgcccccgc cggtgaggta ggtcccgagc tgcgtctgat cggcccccgt
     2041 gagcgtgcag gagaagtcgg cgcccgtgaa ccagatgacg gcgtcgtact cggacatttc
     2101 cgccgcgttc gggccgttgt tgcacccggg tggagcggtg ggcaccgtgt ggaccgtccg
     2161 gttcgtgaac ccgagacttt ggagcgcgtt cgcgtacgcg gtcaggaagc ttgcgcccct
     2221 gtcgtcgtcg acgagcaaga tgggatctgt tcccgagacc cccatccagt cgagcgcgga
     2281 cgagaggatc gcctcgcgat gcgccccgga ggtgatcccc tccagcccga aggtgtggaa
     2341 ggccgagcgg tacgcgacgc cggcccccgt gtccggtcct acgagcaccg ccttggcgaa
     2401 gcgggggacg gtggacagga tgtcgtcccc gggagcgccc agatccacgc tcacggcccc
     2461 gaagttggag aacggtgcca gcgcgtcggt atcggtcgtc gcggcgacgg agatcacgtt
     2521 ggcgttcgtg tagctggcag gataatgggg cgtggcgtcg ttgtcgtctc cctccccgtc
     2581 gctcccgccg tttccggctg ccgcgacgaa gaggacgccc gcgccgttcg cctgctcgat
     2641 cttcgcctgc agcatcccgc tgaagccccc gcctccccag gagttgttcg tgaggtgcgc
     2701 gccgttgtcg atcgcgtagt ccagcgcctc gatcgcggcc gaggtggctc ctccctgagg
     2761 gccgaggaac ttcaggggca tgatcgtcgc ctggctcacc cccgcgacgt gggtcgcgtt
     2821 gtcgatcgtc gcggcgatcg ttcccgccac atgggtcccg tggtcgtcgg gctgattcgc
     2881 gtcgaacacg gtcgcgtcgt cgttgaagaa gtcccatccg tggacgtcgt cgacgtagcc
     2941 gttcatatcg tcgtccacgc cgttggtctc cttgccgccc ccgctctcgc ccgggttcgt
     3001 ccagatctgc gcctggaggt cggggtggtc gatccagacc ccggtgtcga tgacggccac
     3061 cacgacgtcg gacgaatccg cggcgacggc ccacccctcc ggagcgtcga tatcggcgtc
     3121 cggcgtcccg cccgtctggc cctcgttgtg gagcccccag agccactcga actcgggatc
     3181 gtcggggatc ccgtccgggt accagaggag gttcggttcg gcggctcgga tggcgacgtc
     3241 cgccgcgacc cgagccgcga cctcgggggg ctggccctgc cccgggaacc gcacgagctc
     3301 gagatcgggg aggggcagcc ccttgacccg ggtcaagccg tgtcgggccc gggccgcggc
     3361 ccgctcgacg gccggcgtcc ccggtcggaa ctggatcagc agctccccgg gaacgaaggc
     3421 cggtcgcgac gacgcccgcg ggggctgcgg ttgggtcgag gcggcgggcg gcgcccagag
     3481 cagggctccg gccgtgaggc tgagggtgat gccgattgcg agggctctgg acggtcgcat
     3541 gtacctcggg ggatcgggtc cgggctcgac cagcataccg gcgaggacgc cgatcgagcc
     3601 cgaccttcat atcggcccct gaaggagcac gcttgaccgg tacgagctca gcccccgacg
     3661 tcggtcgcct ggcgcaggcc cgccagcgcc ctcgacaggc tcgtggccag gggccggccc
     3721 cgcgtgaggc gcatgagccc gacgatcgtc ttccccttcg ccccaacgcc ggtcctgctc
     3781 cagatcacgt gcatccgact gccgcccccc gccacgggct cgacgcgcgc ggacatgaag
     3841 ctccccggag cgcagaagtt gctctctcga gcggtccagg tcacggtgcc gggggtggac
     3901 cagtcgtact cctccagcgc ccagagcctc ggcttccgct gcccctcgcg caccagcgcc
     3961 gaggtcgcgt ggagctcgac gaccctgtag gcgtcgggat ccagctcggg ccagatctcg
     4021 gggcgtcggt ccgagaagtc cgtgagcgcc gcgagcacgc gctcggcggt gacgggagcg
     4081 ccgacggtga agcgaagctc ggtcatcggt cgccaccccg ctcgtcgatc acgatgtcgt
     4141 atgtgtcgac ggccacgatc tcctcgtcgt acgtcgccgc cgtcttggcg cgcatctcct
     4201 cggcggccct ctcgctggac cgaagggcct cctcggtctc ccagagcgtg atcgccatcg
     4261 acctccccgt cccgcgatcc gcgaggacca gcatcccggc gaagccgtcg agctcccgga
     4321 gcgcgggagc gacgttctcc cgcgcgttcc gggaggcctc gtcgaagttc tcgccggacc
     4381 ccttgaagac ggtcactcga gcgagcatcc gcaccacctc cttgtgagga ggatggcgcg
     4441 cgcgctcgcc cgcccgcatc ggtaggactg cccattcctc cggttagggg cccaggaggt
     4501 cgtgctccat ggcgaagacc gctgctgagg cgcgggtgga gctgccgatc ttcgcgtaga
     4561 tgtgctggac gtggtgctcg gccgtgcggg gcgagatgaa gagccgcttc gcgatctcct
     4621 tgttcgacag cccccgggcg accaggcgga ggacctcgac ctcgcggtcg ctgaggccgg
     4681 cgggccgctc ggccttgacc cgcacgcccg tgtgcccgcc ggcctcagcg accgctcgag
     4741 cgcagtcggg gtccaggcgt ccctcggagg ccgcggccgc gatcgcctcc gcggcctcct
     4801 ccggggcccg ggccggtcgg tggggtcgtt cctgcgtcat cgcctggtag gcgtcggcgg
     4861 ccgccaggat gcgggccgcg gcggggatct cccctccgga ggccccgtgg tgatagccgc
     4921 tcccgtcttg acgctcgtgg tgcgcccccg cgatcgggat cagcggcgcc agggccgcgg
     4981 agtgagccag gatccgctcg gtgtggtacg ggtgcgtccg gaccatctcc cactcggcgg
     5041 tcgtgagcgg ccccggcttg tcccagatcc cgttggagac cccggtgcgg cccacgtcgt
     5101 gcatcagggc cgagaggcgc agctcatcca ggtcgtcctc gctcatgccg agcgatcgtc
     5161 cgccttcgac cgcgagctcc gcgacgcctg tggagtgccc cagcgtgtac ggcgacttca
     5221 ggtccaccat gtcggcgaag gtccgcgcca cgcggtccag ctcggcggcc ccgaccaccg
     5281 ccacgggctc gggctcggcc gcgagcaccg cttcccacgg gtcctccgcc gccccctccg
     5341 ccaggagctg aggccccatc cgccggaacg cctccgcgac agccgggtcg aaccacccgc
     5401 ctgcgcgccg agccgccatc gccagcgcgg cttcgggccc gccggcccga tcgaagatca
     5461 cggcctgcgt ggccacctcg gcgatcctcg ccgggaggac gatgtcctcc ccggatgcgc
     5521 cctcgggcgc gccggtgccg tcccaccgct ccatgacctg gtagagggcc cgctcgaccc
     5581 ccgtccccag ccccagccgc gaggcgaggt gcgagccgac ctcgcagatc gcccggagga
     5641 tgcccgcgtc cgccttccgg cccgccatga gggcgcgggc cagcagccgg ggacggcgga
     5701 tcccggcgcc tcgcccggtg gcgagcagca gcttcactat ctcgcgggcc tcgccgaagt
     5761 cggcggcctc ggacggcggc cgcgacgcga gctcgtcccc gccgaacagg taggcctctt
     5821 cgtgggcggt cgccgtgcac cccagatgcc ggaggagcgt gacgaggtag acctcgcgaa
     5881 cctccgcctc gggcagcccg agtcctctgg ccatccgggt ggccaggagg caggaccgcg
     5941 cggccttctc gggctcctgc cccatcccga tgtcggtggc gaccgagatg gccacgagca
     6001 gatcggcgag gcgcagctga tcggtcacgc tcagcggcag actagcgcct cagcttccag
     6061 agctcgtagc cgctggaccc gtcgtaggtc ccgaagagga accacttccc caggggtgtg
     6121 aagtagccgg ggtacgccgc ccccgggccg gggttgaggt ccctgagccg tctcgtcccc
     6181 ttcttcgtgc cgtcggtgcg ccagagctcg ggcccggtgg tcccgtcgtc cgccttgaac
     6241 agggaccagt tcccgaccgt cgtgaacttg tcggggatcg acgagccggg cccggggttg
     6301 atgtccttca gacgcctcgt ccccttcttc gtgccgtccg tgcgccacag ctcggtgccg
     6361 gcgaccccat cgttcgcctg aaacagcatg atcttgccga acctggccac gtcgtcgatc
     6421 tcggagtcgc ccggccccgg gttgatgtcc ttcacgcgcc tcgttccctt cttcgtgccg
     6481 tcggttcgcc agagctcccg tccccccgga ccgtcggcac ggaagaggat catcttcccg
     6541 tacgcggtga gacgatccgg gaaggagttc ccgccgagga agatgtcctt gaccagccgt
     6601 gtccccttct tcgtgccgtc ggtccgccag agctcgttcc ccgtggcatc gtgcgtggcc
     6661 gagaagatga cgcgcttgcc cagccgcacg aggttgccgg gtgaggagtc tccgggcccg
     6721 ggacggatgt ccttcaccaa tcgggtgccc ttcctggtgc cgtcggtcct ccagagctcg
     6781 atgctcaagg tcccgttcga tgcggagaag aggagcttct ttcccaagcg gacgaagtcg
     6841 tgcggctgcg cgaagctcgg ccccgggttg atgtccttca cccgcctggt ccccttcttc
     6901 gtgccgtcgg tccgccacag ctccatcccc gtcgagccgt cgttggcggt gaagaggatc
     6961 cgcttcccga gccgcgtgag cccttgtgga gaggaatcgc cgggtcctgg gttgatgtcc
     7021 ttcacccgcc tggtcccctt cttcgtgccg tccgtgcgcc acagctccca gcccgtggcg
     7081 ccgtcgtccg cctggaagaa gaggatgcct cccatcagct cgacctccgc ggggaccgag
     7141 gaccccggcc ccgggttgat gtccttcacc cgccgggtcc ccttccgcgt cccgtccgtc
     7201 cgccagagct cggtcccggc caccccgtcg tcggccgaga agaggagcca gcgtccgacg
     7261 cgtctgaagt cgatgccctc gtcgttcaag gaactgccgg ggccaggctt gatgtccttc
     7321 acgggcctga tcctcacagc agccgtggcc atgaggggcg tcgtgaggca cagggcgacc
     7381 gtcagcgcgg cgacggcaga agctcttggg ctcgtcgctc tcaatcgagg tcctcgtgtg
     7441 ctcggatcgc tcggacccta acacccaccc cccgacccgt ccaggcttcc tcttctcgag
     7501 cggtcacggt agattcggcg gccgatggat gcgatctact tctccggcgc cgacgagttc
     7561 cgtgcgtgga tggaggagaa ccaccacacc gccgccgagc tctacgtggg tttccacaag
     7621 aagggggcgg ggatccccag catgacctgg gaggagtcgg tggacgtggc cctgtgcttc
     7681 ggatggatcg acggggtgcg caggagcgtc gacgacgagc gctacgtcat ccggttcacg
     7741 ccccggaggc ccgggagcgt ctggagcgcg aggaacatcg agcgggccaa ggctctgatc
     7801 gaggagggcc gcatgatccc cgccggcctg gcggcgttcg aggcccggag ggaagatcgg
     7861 tccccctact cctacgagcg acgcgcgtcg atcaggctcg acgaggatct ggagcggcgg
     7921 ttccgccgcg cctcgaaggc ctgggagcgg ttccaggcca tggccccctc gtaccggaag
     7981 tccgcgctcc actgggtgat gagcgcgaag aggcacgaga cgcgggagcg ccggctggag
     8041 cgactgatcg cggactcggc cgccggccgc agggtccctc cgctcgctcc gagggagcgg
     8101 cgaggctgag ggcagcacgc cggcccgacc gagcgacgag gtctcgctga gcgaaccagg
     8161 gatcatctcg ctcccgaccg atggttcgac ggcgccaggg gagtctctac ttgacgtcgg
     8221 acgggaagcg atgtagttta ccgctcggtt atttaaccgg gaggtgtcat gggcaccgcg
     8281 acgagagaga ggctggacgc gacgttctcc gccctggccg accccacgcg gcgggcgatc
     8341 ctgacgcgtc tgtcgcaggg tgaggcctcg gtcacgcagc tggcggctcc cttcgacatg
     8401 acgctgccgg ccgtctccaa gcacctgaag gtgttggagc gggccgggct gatcgcccgc
     8461 agcagagagc ggcagtggag gccggcgcgg ctggccgccg agccgctgaa ggaggccgcc
     8521 gactggctgg agcggtatcg gcggttctgg gaggagagct acgaccggct cgacgagtac
     8581 ctggacgagc tccaggggcg acggaaggag gaggaagatg gggaaccggc tgcaggtctc
     8641 gacccccacg gaccgtgaga tcgtgatgac ccgggtgttc gacgcgccgc gggacctcgt
     8701 gttcgaggcc catacttcgt gcgagcacct ccgcaactgg tggggcccga gggcgatgga
     8761 gatctcggag tgcgaggtcg acttctggga gggaggggcg tggcggatcg tccaccgggg
     8821 ccccgatggg gaggagtacg ggttcagcgg cgagtaccgc gagatcgtgc ggcccgagcg
     8881 gatcacgtgg acgttcgagt tcgacggggc gccggggagc gtgtcggtgg agacccttac
     8941 cctcgaggag cacgacggta agaccaccgt gacggccacc gccgtgtacg actcgaagga
     9001 ggcccgagac gcgatgatcg agtcgggcat ggagacgggc gccgcggaaa cctacgagcg
     9061 cctcgacgag tacctggagg ctctgcggga gcgggcgccg agctgaagat cccacggccg
     9121 ggaacatccg ccgagccctc cgggttgtgg caggattgcg gccgttgttg gagggagggt
     9181 gatgatccgg acatcgggat tggtgaagcg gtacgggaag gtgacggccc tcgatgggct
     9241 cgacctcgag gtgcccaagg gcacggttct agggctcctc gggccgaacg gagcggggaa
     9301 gaccaccgcg gtgcggatcc tcaccacgct cctcgacgcg gacgagggga ctgccgaggt
     9361 ggcgggcctc gacgtccgcc ggcaggccac cgaagtccgt cgggtgatcg ggctgtcggg
     9421 ccagaccgcc gccgtggacg agtacctcac cggctacgag aacctggaga tggtggggcg
     9481 gctgtaccac ctgggcgggc ccaggtcccg agccagggcg agggagctcc tcgagcagtt
     9541 cgacctgcag gaggccggga accgcccggc caagacgtat tcgggcggga tgcgtcgacg
     9601 gctcgacctg gccgccgctc tcgtcgcgga gccggaggtg ctcttcctcg acgagccgac
     9661 gacgggcctc gaccctcgga gccgcgtcca gatgtgggtt acgatccaaa gcctggttca
     9721 cgccgggtcc acggttctcc tcaccacgca gtacatggag gaggccgacc gcctggccga
     9781 cgacatcgtc gtgatcgacc gcggccagaa gatcgcccac ggcacggccg accagctcaa
     9841 gctgcaggtc ggcggggagc ggctcgagct cgtgctgggc gacatgtccg acctggagag
     9901 cgcccgctcg atc
//
LOCUS       DASUTZ010000213         2173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_463033, whole genome shotgun sequence.
ACCESSION   DASUTZ010000213 DASUTZ010000000
VERSION     DASUTZ010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463033"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1579)
                     /locus_tag="VF097_09240"
     CDS             complement(143..1579)
                     /locus_tag="VF097_09240"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX6263016.1"
                     /translation="MAERRFLERLARLAVPALIGMVGAWLALLMAGRHDYDLGPFRVE
                     FFGRPGSSATELALPPFGRLQADTHLAPLKLSATLQEVAAVELGDIVQHKGVGGLTLE
                     IEEEAPGVLVSYVLRSLAVVLAGAAVAALLVFRARWRPVIVAVVTSGLVFGGMAGISW
                     LTFRPEAFLSPTFSGSLALAPRLVGPIRDAGGRIEDFRAELERLVGGAVSAYTTIAAT
                     PPTTGADIVALHISDVHLSPLGMDFAQRLASSFEVDLVLDTGDLTSFGSSLEQGITSR
                     IREFGVPYVFVRGNHDSALTVNEVLAQPNAIVLRHELREVAGLRIYGAPHPLFTPALE
                     RDYPGEEIEETLLAVGEVVSEEVSAASPDLLVVHDDRMAEASIGLVPVVASGHFHQTT
                     ARVVEGTILLRVGTTGAGGLDTFTAPQPIPLSAQLLYFDGDPVQLVAWDTVLLDPITR
                     DLTVRRHLPTEIGEEEEPAPQGEPAPTP"
     gene            complement(1617..>2173)
                     /locus_tag="VF097_09245"
     CDS             complement(1617..>2173)
                     /locus_tag="VF097_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263017.1"
                     /translation="TESFRTGRAPAGRRYFAQIQTDLPEPHSETVDVVVDEAASIVRV
                     HIATGTTDLLLAPAGGVLAGTRNGEPIELRWGPDRHLDYLSPVFNAISAARLDGTAEI
                     DVLYLDPVTCEPRDMIQRYEWLGPERIATPVGAFEAGAWRYTAVASGFSRRLWVAADL
                     VVAYETVFELIEYEPGQTGPFPSA"
BASE COUNT          344 a          746 c          799 g          284 t
ORIGIN      
        1 gagcgggaag aagaggttct tcctctgggc cgcgatcctc ggcgggatcg gcgccgtcgc
       61 ggcggtcttc aggcgccgcc ggtcccagag cgtcgacgag agcgagtggc aggagctccc
      121 tccggccgac gggagctgat cttcaaggtg tgggagccgg ctccccctgg ggggccggct
      181 cctcctcttc cccgatctcc gtgggaaggt gacgccgcac cgtgaggtcc cgagtgatcg
      241 ggtcgagcag gaccgtgtcc caggccacga gctgcacggg gtcgccgtcg aagtagagca
      301 gctgcgccga gagcgggatc ggctggggcg ctgtgaaggt gtcgagcccc ccggcccccg
      361 tcgtccccac ccgcagcagg atcgtcccct ccaccactcg cgctgtcgtc tggtggaagt
      421 ggccgctggc caccacggga accagaccga tcgaggcctc ggccatgcgg tcgtcgtgca
      481 ccacgagcag atccggactc gccgccgaga cctcctccga caccacctct cccaccgcca
      541 gcagggtctc ctcgatctcc tcgcccgggt agtcgcgctc gagcgcgggt gtgaagaggg
      601 ggtggggggc gccgtagata cggagacccg cgacctctcg gagctcgtgc ctgaggacga
      661 tcgcgttggg ctgagccagc acctcgttca ccgtgagcgc cgagtcgtgg ttcccgcgca
      721 cgaacacgta cggcacgccg aactcccgga tgcgagaggt gatcccctgc tccaacgagc
      781 tcccgaacga ggtgaggtct cccgtgtcca ggacgagatc cacctcgaac gaggaggcca
      841 ggcgctgcgc gaagtccatc cccagcgggg agaggtgcac gtcggagatg tggagcgcca
      901 cgatgtcggc ccccgtcgtg gggggcgtgg ccgcgatcgt cgtgtacgcg gagacggctc
      961 cgccgaccag ccgctcgagc tcggcgcgga agtcctcgat ccgccctccc gcatcccgga
     1021 tcggtcccac gagcctcggg gccagggcga gggagcccga gaaggtcggg ctgaggaagg
     1081 cctccggccg gaacgtgagc caggagatgc cggccatgcc tccgaagacg agtcccgagg
     1141 tgacgaccgc cacgatgacc ggccgccacc gcgcgcggaa gacgagcaga gccgcgacgg
     1201 ccgcccccgc caggacgacg gcgagcgacc tgaggacgta cgagacgagc acccccgggg
     1261 cctcttcctc gatctccagc gtgagccctc cgaccccttt gtgctggacg atgtcgccga
     1321 gctcgacggc ggcgacctcc tgaagggtgg ccgagagctt cagcggagcg agatgggtgt
     1381 ccgcctgcag cctgccgaac ggcgggaggg cgagctcggt cgcgctggag cccgggcgac
     1441 cgaagaactc gacccggaac gggcccaggt cgtagtcgtg ccggcccgcc atgagcagcg
     1501 cgagccacgc tcccaccatg ccgatgaggg cgggcaccgc gagccgcgcg agccgctcca
     1561 ggaagcgacg ctcggccacg ggctccactg tacccggagg aggcagacca cccgctctac
     1621 gcggagggga agggcccggt ctggccgggc tcgtactcga tcagctcaaa gaccgtctcg
     1681 taggcgacca cgaggtcggc ggcgacccag aggcgtcgcg agaagccgct ggcgacggcg
     1741 gtgtagcgcc aggcgccggc ctcgaacgca cccaccggcg tggcgatgcg ctcgggcccc
     1801 agccactcgt agcgctggat catgtctcgc ggctcgcagg tgaccgggtc gaggtagagc
     1861 acgtcgatct cggcggtgcc gtcgagacga gccgcgctga tcgcgttgaa aacgggggag
     1921 aggtagtcga ggtgacggtc ggggccccac cggagctcga tgggctcacc gttcctcgtc
     1981 ccggccagga cgccgcccgc cggagccagc aggaggtccg tcgtgcccgt ggcgatgtgg
     2041 acgcggacga tcgaggcggc ctcgtctacg acgacgtcca cggtctcgct gtgcggctcg
     2101 gggaggtcgg tctggatctg agcgaagtag cggcggccgg cgggggcgcg gccggtacgg
     2161 aaggattccg tac
//
LOCUS       DASUTZ010000214         5692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_74764, whole genome shotgun sequence.
ACCESSION   DASUTZ010000214 DASUTZ010000000
VERSION     DASUTZ010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74764"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..843)
                     /locus_tag="VF097_09250"
     CDS             complement(91..843)
                     /locus_tag="VF097_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019433781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6263018.1"
                     /translation="MNDDGQRTGAGTPLIELRGVYASYGPVEVLRDISLQVQEGEITC
                     LLGANAAGKTTTLKTILGMVKPTAGEIYLRGERIDSDNTQRIVAQGLTMVPEGRRLFG
                     KMSVRENLEIGTQLRKDEDGIQRDMERVLNLFPRLRERLDQKAGTLSGGEQQMCAIGR
                     ALMAGPKLLLMDEPSMGLAPILVEQVFEVIQEINNQGTTIFLVEQNANMALAIADRGY
                     VLQTGEVVMSDTAKVLRENPKTREAYLGELTE"
     gene            complement(840..1631)
                     /locus_tag="VF097_09255"
     CDS             complement(840..1631)
                     /locus_tag="VF097_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6263019.1"
                     /translation="MTEQLLVVKELVKHFGGVRAVDGVDFHADEKEIVSVIGPNGAGK
                     TTLFNVVTGFYEADSGTIDFDGFNLLGLKPNRITALGIARTFQQIRLFPNLTVLENAM
                     VGQHARTRSGAFGAIFRPPWTRREEHEIAERAREALAFFGDSLLPFENMMAFELSYAD
                     RRRLEIARAMATHPKLLLLDEPTAGMNPVEKHGLVTLIGRLREEQGFTVVLIEHDMPV
                     VKNVSDRVVALDHGQEIAQGSYDEVSNNEQVIEAYLGKKKAVESA"
     gene            complement(1628..3460)
                     /locus_tag="VF097_09260"
     CDS             complement(1628..3460)
                     /locus_tag="VF097_09260"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6263020.1"
                     /translation="MMATSAAAQRQRTAAETSSWFTRSLLTTGVIWGLVAVYFMAVGM
                     VESFQSLAVITDVLSLGRTLLIIAFLVAGYMGSRGLLRRGHMAEGQRLMGGLVAGAVA
                     GAVAALVLLVVDLIGVEHVRRMFQSFTANLVNNILSFGEGVAFGALALVVGGAALGLG
                     GAALQILPERLRRATVAGLGTALIVSLAQPFIVAVFRNLESNFGIDIPLSWLFTGTGL
                     AYTGLALMFVAGFVVSRWRTRTAPDRETRRPEELTGERSLLDLVRGRGLKAVGWLALI
                     ALLILMPFIVGQFLSQVINTVGIYVLLGLGLNIVVGFAGLLDLGYVAFFAAGAYATAI
                     LTSTASFLEGPFTFWVALPLVLLIAVFSGILIGAPVLRLRGDYLAIVTLGFGEIARVF
                     IASIWLQDFLGGAQGILRIPGPPFFGVNLRDPLNLYYIILAFCLIAAFVAWRLKESRV
                     GRAWAAMREDETVAEAMGISVINYKLLAFAIGAGIGCLGGAFFAIQVGSVFPNSMDIL
                     VSINALSVIVLGGMGSIPGVVLGAFGLVGLPELLREFEEFRLLIYGAVLVALMILKPE
                     GLLPEVRRKQELHEREMEEQYEDRVGEGAEPVLRAEASDDEETT"
     gene            complement(3457..4581)
                     /locus_tag="VF097_09265"
     CDS             complement(3457..4581)
                     /locus_tag="VF097_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6263021.1"
                     /translation="MADDTETKAPEPKEATPGRFQRGVMRLPIRTFILWLISIGLALF
                     IIWGTTRTLSLEVSTFSGEGWKRLISDGVARGAIYALIAMGYTLVYGILFMINFAHGE
                     VFMSGAFTAFFMARWLAQTGFLNENPIISILILFAISMTVSMSVAVVLERVAYRPLRG
                     APRLVPLITAIGASLFLQNTFRGFYGASAEAYPTIDLLAGSIEILGWPMRKIQLVVVL
                     AAGALMLGLFLFIRKTRTGRAMRAVGEDKEIAALMGIDVDRTIMSTFAIGGLLAGAAG
                     ILFVYLFRGGSVNFFMGFFPGIKAFTAAVLGGIGNVVGAGVGGLLLGVFEAVGPFLLL
                     EGFNVPSPNQLQLVIAFSILVLVLIFRPGGLLGSPDEERA"
     gene            complement(4674..>5692)
                     /locus_tag="VF097_09270"
     CDS             complement(4674..>5692)
                     /locus_tag="VF097_09270"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX6263022.1"
                     /translation="GHDIELVNEDDGCSAEGGTAGATALASDPSIVAVVGTSCSSAGE
                     PASQILSDAQVPIVSPSNTAPSLTDPATHQEFYLRTAHNDKVQGAAMAQFVSEELGVS
                     TAATIHDGSPYADQLQQVFADTLTNEYGGSITTQEGVQVGETDFGPLLNSIAADAPDF
                     LYFPIFVAEGGLITAQTREIGQLDDTDLGGADGMLTPDWIDAAGADNAEGVFLSGPDL
                     GFAGGFYQDEFLPAYNDQFGEPTSVFHAHSFDAAGMIFQAIEEVAIEDGDALLIPRVE
                     LKDALFSITGYEGIVGTLDCTPSDDAIGDCNEGATITVSQVQDGDFVVVWPADKAQGD
                     IQGA"
BASE COUNT          971 a         1968 c         1879 g          874 t
ORIGIN      
        1 tccacggccg cgcccatcgg cagcagctcg gccaagcgta cggcgtccac gatggagagg
       61 gtcacgggcg cggcggaacc ttccgacgca ctactcggtc agctcgccga ggtaggcctc
      121 gcgggtcttg gggttctccc tcaggacctt ggcggtgtcg gacatgacca cctcgccggt
      181 ctgaaggacg tagccgcggt cggcgatcgc gagagccata ttcgcgttct gctccacgag
      241 gaagatcgtg gtcccctggt tgttgatctc ctggatcacc tcgaacacct gctccacgag
      301 gatgggggcc agccccatcg agggctcgtc catgaggagc agcttggggc cggccatgag
      361 ggcccggccg atggcgcaca tctgctgctc accgccggag agggtccccg ccttctggtc
      421 gaggcgctcc cggagccgcg ggaacaggtt caggacccgc tccatgtcgc gctggatccc
      481 atcctcgtcc ttgcgcagct gggtcccgat ctcgaggttc tcccggacgg acatcttccc
      541 gaagagccgc cgcccctcgg gcaccatcgt tagcccctgc gccacgatcc gctgggtgtt
      601 gtcgctgtcg atccgctcgc cccgcaggta gatctcaccg gccgtcggct tcaccatgcc
      661 taggatcgtc ttgagcgttg tcgtcttccc ggcggcgttc gcccccagga gacaggtgat
      721 ctcaccctct tggacctgga ggctgatatc tcgcagtacc tccacgggcc cgtagctggc
      781 gtagacgccc cggagctcga tgagcggggt cccggccccc gtccgctggc cgtcgtcgtt
      841 cacgccgact ccaccgcctt cttcttcccg aggtacgcct cgatcacctg ctcgttgttc
      901 gagacttcat cgtaggagcc ctgggcgatc tcctgcccgt ggtcgagggc caccacccgg
      961 tccgagacgt tcttcaccac cggcatgtcg tgctcgatca ggaccacggt gaagccctgc
     1021 tcctcgcgga ggcggccgat cagcgtgacg aggccgtgct tctcgacggg gttcatgccc
     1081 gcggtcggct cgtcgagcag gaggagcttg gggtgcgtag ccatcgcgcg agcgatctcc
     1141 aggcggcggc ggtcggcgta ggagagctcg aacgccatca tgttttcgaa cggcaggagg
     1201 ctgtcgccga agaacgccag cgcctcgcgg gcccgctccg cgatctcgtg ctcctcccgc
     1261 ctcgtccacg ggggacggaa gatcgccccg aaggcgcccg agcgggtccg ggcgtgctgc
     1321 cccaccatgg cgttctcgag gacggtcagg ttggggaaca ggcggatctg ctggaacgtc
     1381 ctggcgatcc cgagcgccgt gatgcggttt ggcttcaggc ccagcaggtt gaatccgtcg
     1441 aagtcgatcg tgccgctgtc ggcctcgtag aagccggtga cgacgttgaa cagcgtggtc
     1501 ttgccggcac cgttggggcc gatcacgctg acgatctcct tctcgtcggc gtggaagtcc
     1561 acaccgtcca cggcgcgaac accgccgaag tgcttcacca gctccttgac gaccagcagc
     1621 tgctcggtca cgtcgtctcc tcgtcgtcgg atgcctcggc ccggagcacg ggctcggcgc
     1681 cctccccgac ccgatcctcg tactgctcct ccatctcgcg ctcgtggagc tcctgcttcc
     1741 ttcggacctc gggaaggagg ccctcgggct tgaggatcat cagcgcgacg agcacggcac
     1801 cgtagatcag gagccggaac tcctcgaact cacgcagcag ctcggggagg cccacgagcc
     1861 cgaaggcgcc caggacgacc cccgggatac tccccatccc tccgaggacg atgacggaca
     1921 gcgcgttgat cgacacgagg atgtccatcg agttcgggaa caccgatccc acctggatgg
     1981 cgaagaacgc gccgcccagg cagccgatcc cggcgccgat cgcgaaggcc agcagcttgt
     2041 agttgatcac gctgatgccc atggcctcgg cgacggtctc gtcctcgcgc atcgccgccc
     2101 acgcgcgccc cacccgagat tccttcagcc gccacgccac gaacgcggcg atgaggcaga
     2161 aggccaggat gatgtagtac aggttcaagg ggtcgcgcag gttgaccccg aagaaaggcg
     2221 gcccggggat gcgcaggatg ccctgcgcgc cgcccaggaa gtcctgcagc cagatcgagg
     2281 cgatgaacac gcgggcgatc tcaccgaacc cgagggtgac gatcgccagg tagtctcctc
     2341 ggagcctgag cacgggggcg ccgatcagga tgccgctgaa cacggcgatc aggaggacga
     2401 gcgggagggc cacccagaag gtgaacgggc cctccaggaa cgaggctgtg gaggtgagga
     2461 tggcggtggc gtaggccccg gccgcgaaga acgccacgta cccaaggtcc agaagcccgg
     2521 cgaagccgac gacgatgttc aagccgaggc cgaggaggac gtagataccc acggtgttga
     2581 tgacttggct gaggaactgc ccgacgatga acggcatcag gatcagcaaa gcgatgaggg
     2641 cgagccagcc caccgccttc aacccgcgac ccctgaccag gtcgagcagc gaccgctctc
     2701 cggtcagctc ctcgggcctc ctcgtctcgc ggtccggagc ggtgcgggta cgccaccgcg
     2761 acacgacgaa ccccgccacg aacatcagcg cgagcccggt gtaggcgagg cctgtgccgg
     2821 tgaacagcca cgagagcggg atgtcgatgc cgaagttcga ctcgaggttc cggaagaccg
     2881 ccacgatgaa cggctgcgcg aggctcacga tgagcgcggt gccgagcccc gccaccgtgg
     2941 cccgccgcag gcgctcgggc aggatctgga gggctgcccc gcccagcccg agcgcggctc
     3001 ctccgaccac cagagcgagg gcgccaaacg ccacgccctc cccgaacgag aggatattgt
     3061 tcacgaggtt cgccgtgaac gactggaaca tgcgccgtac gtgctccacg ccgatcagat
     3121 cgacgacgag cagcacgagg gcagccacgg cccctgcaac cgcccctgcc acgagcccgc
     3181 ccatgagccg ttgcccctcc gccatgtggc cccggcgcag gagccccctc gagcccatgt
     3241 acccggccac gaggaaggcg atgatcagca gggtccgtcc cagcgacagc acgtcggtga
     3301 tcacggccag agactggaag ctctccacca tccccacggc catgaagtac acagcgacga
     3361 gcccccagat cacgcctgtc gtgagaaggc tccgagtgaa ccacgacgat gtctccgcgg
     3421 ccgtgcgctg gcgctgagcc gccgccgagg tcgccatcat gcacgctcct catccgggga
     3481 accgagcagg ccgccgggtc ggaagatcag cacgagcaca aggatgctga acgcgatcac
     3541 gagctgcagc tggttcggcg agggcacgtt gaacccctcg aggagcagga agggcccgac
     3601 cgcctcgaag accccgagca gcagccctcc gacaccggcc cccaccacgt tcccgatgcc
     3661 tccgaggacc gcggcagtga acgccttgat ccccgggaag aagcccatga agaagttcac
     3721 cgaacccccg cggaagaggt agacgaagag gatgcccgcc gccccggcca ggagcccccc
     3781 gatggcgaag gtgctcatga tcgtccggtc gacgtcgatg cccatgaggg cggcgatctc
     3841 cttgtcctcc ccaaccgcgc gcatcgccct cccggtcctc gtcttcctga tgaagaggaa
     3901 gagccccagc atcagcgcgc cggccgccag gaccaccacg agctggatct tccgcatcgg
     3961 ccagccgagg atctcgatcg acccggccaa aaggtcgatc gtcgggtagg cttcggcgct
     4021 cgcgccgtag aatccgcgga aggtgttctg gaggaacaac gaggccccga tcgcggtgat
     4081 cagaggcacc agtctgggag cgcctcggag aggtcgatac gcgacgcgct ccagaacgac
     4141 ggcgacgctc atcgagaccg tcatcgagat cgcgaacagg atcaggatgg agatgatcgg
     4201 gttctcgttg aggaaccccg tctgcgcgag ccaccgagcc atgaagaacg cggtgaacgc
     4261 accgctcatg aacacctcgc cgtgggcgaa gttgatcatg aacaggatcc cgtacacgag
     4321 cgtgtagccc atcgcgatca gcgcgtagat cgcgcccctg gcgacgccgt cgctgatgag
     4381 gcgcttccac ccctctccgc tgaacgtgct cacctcgagg gagagcgtgc gggtcgtccc
     4441 ccagatgatg aagagcgcca ggccgatgga gatgagccac aggatgaacg tgcggatcgg
     4501 gaggcgcatc accccgcgct ggaagcgccc gggtgtggcc tccttgggct cgggggcctt
     4561 ggtctccgtg tcgtcggcca tcagctctcg tgaaggtctg gggtagcggc cgcgggtgag
     4621 cctttcggct cacccgcggc caaccacccg tcgggtcgtt acccgttacc ggttcacgcc
     4681 ccctggatgt cgccctgggc cttgtcggcc ggccacacga cgacgaagtc gccgtcctgg
     4741 acctggctga ccgtgatcgt ggcgccctcg ttgcagtcgc cgatcgcgtc gtcgctcggc
     4801 gtgcagtcga gggttccgac gatgccctcg tacccggtga tcgagaacag cgcgtccttc
     4861 agctcgacgc gcgggatcag cagcgcgtcc ccgtcctcga tcgcgacctc ctcgatcgcc
     4921 tggaagatca tgcccgcggc gtcgaaggag tgcgcatgga acaccgacgt gggctcaccg
     4981 aactggtcgt tgtaggcggg caggaactcg tcctggtaga agccgcccgc gaacccgagg
     5041 tccggcccgg agaggaagac gccttcggcg ttgtccgcgc cggccgcgtc gatccagtcc
     5101 ggggtgagca tgccgtcggc cccacccagg tcggtgtcgt ccagctggcc gatctcacgg
     5161 gtctgggcgg tgatgaggcc gccctccgcc acgaagatcg ggaagtacag gaagtccggc
     5221 gcgtcggccg cgatgctgtt gagcagcggg ccgaagtccg tctctcccac ctggacgcct
     5281 tcctgcgtgg tgatcgagcc gccgtactcg ttcgtcagcg tgtccgcgaa cacctgctgg
     5341 agctggtcgg cgtaggggct tccatcgtgg atggtcgccg cggtgctcac gccgagctcc
     5401 tcggacacga actgcgccat ggccgcgccc tggaccttgt cgttgtgcgc ggtgcgcagg
     5461 tagaactcct ggtgggtcgc cgggtccgtc agcgacggcg ccgtgttgga cggcgacacg
     5521 atcgggacct gggcgtccga caggatctgc gacgccggct cgccggcgct cgagcagctc
     5581 gtccccacca ccgccacgat ggacggatcg gaggcgagcg ccgtggcacc cgcggtgcca
     5641 ccctcggcgg agcagccgtc gtcctcgttc acgagctcga tgtcgtggcc ga
//
LOCUS       DASUTZ010000215        16433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_334202, whole genome shotgun sequence.
ACCESSION   DASUTZ010000215 DASUTZ010000000
VERSION     DASUTZ010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 16433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16433
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334202"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VF097_09275"
     CDS             complement(<1..400)
                     /locus_tag="VF097_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX6263023.1"
                     /translation="MMSSSDRILPNPLRGTVPAPSTGPDPLAFHRRLPGYEVTPVVEA
                     PTVARRLGVGTVWVKDESRRLGLPSFKILGASWATYRTLVERLGRDVEPWSDVAELSE
                     RLTPLRPLTLAAATDGNHGRAVARMAALLGL"
     gene            complement(408..1409)
                     /locus_tag="VF097_09280"
     CDS             complement(408..1409)
                     /locus_tag="VF097_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX6263024.1"
                     /translation="MEREALTGRDYIETLDWSVEELEEALAVSADLKERFKRGEPHRL
                     LADKTLFMLFLDKSTRTRNAFEAGMTQLGGHAHYLTAEATQVSHGESPKDMGIILSSY
                     GHGIAIRHDLIPYECNAYIREVARWAEVPVLNLQCDVDHPTQTLADLMTLRAKRGADL
                     RGMRVAVSWAYAPSYAKPLSVPQGLAALLPRFGVDVVLAHPPGWELMPELMARAEKAA
                     TEGADSITVTNDMDEAFEGADAVYPKSWGRLDLFTQEEEALAASEPYRGWICDARRME
                     LAKDDALYMHCLPADRGNEVTDEVIDGPRSVVYEEAENRLHTGKALMALTMGGFGAA"
     gene            complement(1423..3807)
                     /gene="pucD"
                     /locus_tag="VF097_09285"
     CDS             complement(1423..3807)
                     /gene="pucD"
                     /locus_tag="VF097_09285"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase subunit D"
                     /protein_id="HEX6263025.1"
                     /translation="MSVRIGERLVRGVGESVRRVDGVPKVRGEFEFGSDLWAEGMLWG
                     HTLRTPHPHARVRSIDVSRAVAAAGVHAVLTSEDVPGHKMYGLEFQDQPVLAIDRVRY
                     QGEPVAAVAAEDPELARRAAALIRVEYEELPVVDDMELAMEPDAPKLHEWGNVLRHVR
                     IAHGDPEAEADVWVEGYYETGMQDQAPLGPEAGLAVPSEDGGVDLYVATQWLHVDHRQ
                     IAGCLGLPDEKLRLHLAGVGGAFGAREDVSMQIHASMLALRTGRPVKMSYGREESFFG
                     HVHRHPARIWMRHGATSDGRLVSVRARIVIDGGAYSSSSAAVIANATTFAAGPYEVPN
                     ALLEGTCVYTNNPPCGAMRGFGAVQAAVGYESQMDRLAAALAMDPVELRERNALSTGS
                     LLPTGQVIRGSAPVREVIERCARIPMPPEPEGMREPVTLPGGANNVGRGEGLRRGVGF
                     AVGYKNIAYSGGFDDSAAARVRLSAGPDGPVAEVHSAAAEVGQGVHTILAQIAGTELG
                     VERVELLTPDTGVASAGSSSASRQTMMSGGAVQLACRAVREELFERVRRARPEVGDAP
                     LTLEEGRVLAEGLPVGDLAEFLTDPIEESSVYRHRPTEPFDPDGQADFHVSFAFAAAR
                     AVVEVDEDLGLVRVVEVAAAQDVGRALNPQSVRGQIEGGIAQGLGLALMEEIQLEEGR
                     ILNASFTDYLIPTTLDMPPVVSELVEEPEPGVPFGAKGVGEPSTIVATAAVAAALRDA
                     TGREVNRVPVRPDDLVGIAPPVSTRGWPRVPDVPGQEPVPVYAGVPATELAQKA"
     gene            complement(3804..5162)
                     /locus_tag="VF097_09290"
     CDS             complement(3804..5162)
                     /locus_tag="VF097_09290"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015793324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HEX6263026.1"
                     /translation="MTRLLVEGCEVLVTMDDAGTEIPGGSLLIEDGVIAWVGWGEPPD
                     AEGARRVDGRGAVAIPGLINTHHHLFQTLTRVRAQDEGLFGWLVELYPIWAGLDAEWE
                     LAAARVGLAELALSGCATTTDHHYIFPEGADGLLEAEIEAAREVGLRFHPCRGSMDLG
                     ESDGGLPPDRVVEDTDQVLQETEEAIARFHDPAPGAMLRISVAPCSPFSSTERLMRES
                     AELARRHDVRLHTHIAETRDEERFCLERFGRRPLELLADLGFLGPDVWLAHCVHLDQK
                     DVRRMAESGTSTAWCPSSNLRLGSGTAPARSLLDEGANVGLGVDGSASNDAGHLLGEV
                     RQAMLTARGGDGPAAMSAREALRVATRGGAAALGRDDVGSLEVGKRGDVALFSVEGLG
                     FAGAGSDPVAALLFCSPGRVRDLLVDGHAVVEDGRLTGVDEEEVARRGRRASARALGG
                     RA"
     gene            complement(5159..5629)
                     /locus_tag="VF097_09295"
     CDS             complement(5159..5629)
                     /locus_tag="VF097_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6263027.1"
                     /translation="MLIRTNVNGEWREADAWAGSSLLSFLREDLGLPGSKNACEQGEC
                     GSCSVWLDGELVCSCLVLAAQAHEREVRTVEGLGSDDLLHPVQEAFLETGAVQCGFCT
                     PGLIVAVTDLLQRDPAPSEATVREALSGNLCRCTGYAKILDAVRLAAERMGGTA"
     gene            complement(5639..6514)
                     /locus_tag="VF097_09300"
     CDS             complement(5639..6514)
                     /locus_tag="VF097_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX6263028.1"
                     /translation="MEVLRPRGLQEALAMKAAHPEARPIAGGTDLMVELNFDRDRPEA
                     ILDLWPVPELREHGREDGRIFLGAGVTFTRIMRELPELVPLFQASRTVGSPQIRNRGT
                     IGGNLGTASPAGDALPVLAAYDAEIVVGREAADPRSLPWSEFLRGPKQTALGPEELIL
                     GVRFRPPRGPGSFSKIGVRNAMVISAVGLCLVLDEDDRAVRFALGSVGPTIIRAAEAE
                     ALAARAIAEAGAWESPAAALPDGVLDDVADLVARAARPIDDVRGTAAYRRHACRVLAR
                     RALTWSLGERATGAA"
     gene            complement(6517..7533)
                     /locus_tag="VF097_09305"
     CDS             complement(6517..7533)
                     /locus_tag="VF097_09305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008165109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6263029.1"
                     /translation="MAVSSEGRVALYLQDKHPIRDGMRYVRDAEERGFEAVWQAESRL
                     VREATVPMAAFAAVTDRIKVGSGVVNSWTRNVGLLAATFATLDELAPGRIMLGIGAWW
                     EPLASKVGVRRRKPLTAMREVVEVTRRLLAMERVTFHGEFVEVEDIEIDIVHGDRSPK
                     DVPIYIGATGMRMMELAGEIADGVLLNYLVSPEYDRAAMGALAAGAERAGRSVEDVDR
                     PQLVVCSLDEDREAALDRARELVTQYLGQQPHIMKASGVDQSLLDEIAEVLTWPAGPE
                     EIENAMRLVPDDVVQMITASGTAEECRAKVREYVEAGCTCPVLYPLGDDVRAMLDAFA
                     EGAF"
     gene            complement(7533..8870)
                     /locus_tag="VF097_09310"
     CDS             complement(7533..8870)
                     /locus_tag="VF097_09310"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6263030.1"
                     /translation="MTGGAGLVLTGGTVVRSLDPPIVEAADVRIADGRVAELGSGEGD
                     ATLDCSGCLVIPGNVCAHHHLYSSLARGMPYALEPPESFVQILQRVWWRLDRALDPQG
                     VRWSAAVGGSEALLSGTTTVIDHHASPGDIEGSLDRLASALGDLGIRSVLCYEVTDRD
                     GPEKAEAGIEENRRFLKGGGSPLARGMVGAHASFTLSEDTLAGCVEAARELGAGIHIH
                     VAEDGADQDDARARFGRRVVHRLAEAGALDERALLAHCIHVGPDEAEVIRETGATPVH
                     NPRSNMNNAVGHAPVALLGDRLALGTDGIGGDMFAEGRAAYWRAREAGTASAPSWVLG
                     RLARSARFAGLIFDEPLLGTIERGAPADLVVLAYDPPTPLDAETLAGHWVFGLSARQV
                     RDVVVEGEPVVRDGRLTRVDQSEVAARARESAERLWRRMDEIAEHPFSPSGAA"
     gene            complement(8872..11499)
                     /gene="xdh"
                     /locus_tag="VF097_09315"
     CDS             complement(8872..11499)
                     /gene="xdh"
                     /locus_tag="VF097_09315"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0046415 - urate metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HEX6263031.1"
                     /translation="MSEAAPLEFVLNGRPVSVEVDEGTMLLSVLREGLGLTSMKDGCA
                     PEGSCGACTVMVDGKGVVSCAQRATRVAGKEVTTLEGLPQERRDEWAECFLASGASQC
                     GFCSPGLVMKCEAMLAKNPEPSREEVARAIAGNLCRCTGYVKIIDAIEHAAGTRRGEP
                     LPEVDRSGRVGSRTGRYEGRELALGEKPFVNDMRVPGMLHGAIRFSDHPRARVLRIDT
                     SRAEAHPGVVAVATADDVPGERRQGLITKDWRQLVAEGEEVSCVGDVLAVVAAASRAE
                     AREAAALVDVEYDVLPPVTDPFEAMAEDAPQLHEGGNVLSISEVKRGDVDAALGEAAH
                     VVTETYRTQWVEHAFLEPESSLAVPTEDGGLHFYSQGQGVWDDRRQVASLLAMPEERI
                     RVTQVSNGGAFGAKEDLNVQGHACLLALMTGRPVLVTLSREESIRFHAKRHPLTMEYT
                     VGCDQEGRLLGIRARIVGDTGAYASVGDKVLERAAGHACGPYRVPAVDVEARAVYTNN
                     PPAGAMRGFGVNQVNFAIEGVLDTLAERVGIDGWEIRWRNALETGDVFATGQVLGPGV
                     GIKQTLLAVRDVYRAAPRAGIACGLKNSGVGNGLTEYGKAVLRVEDDGSVTLFHSWTE
                     MGQGVHTALQQIACEELGITSDRIRVAVDTIRDIDTGQCTASRSTVLGGRAVIEAARN
                     LKAELNGGRLEDLAGREFYGEFVVDWTTKLGVENPVTHYAYGWATQVVTLDGEGRIEK
                     VVAAHDVGKVINPTLLEGQIEGAVHMGLGHALSEELVLEGGVPVTDTLKSLHIIPPAG
                     MPPVECIFVEEHQPEGPYGAKGVGEIGLVPTAAAVAGALHAFDGVRRTRLPMKDSPAA
                     RAAVPKLAAAARRSADREG"
     gene            complement(11525..12739)
                     /locus_tag="VF097_09320"
     CDS             complement(11525..12739)
                     /locus_tag="VF097_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HEX6263032.1"
                     /translation="MRLEERFQDEAGALAERTTAFLSDIIAIPSLSRREGDVARRIGD
                     EMTDLGYDEVTIDGFGSVIGRIGDGPVGIVYDSHIDTVDVGERSSWKADPFTPRIEDG
                     VIYGRGASDNKAATATMVYGGALIKRLGIDSSRFTVYVVGTVQEEDCDGLALEYVLTE
                     SIPRPDVVVLGEATELDVYRGHRGRVELAVHTRGRAAHASAPERGINAIYRMAPIIRQ
                     IEELNERLADDPFLGKGTIVLSKIDCRTPSLNAVPDGCSVYMDRRLTVGETLETARKE
                     LEELSAVQEAGAEVEVLQYEGESYTGLTLSTEKYFPTWVVPEDDPAVRAGVEAGERTQ
                     GRTPAVGRWIFSTNGVSSMGKLGITTIGFGPAEEKYAHSADDQCPLDHLAPAVAWYGA
                     FPESYLAATGSS"
     gene            12834..13838
                     /locus_tag="VF097_09325"
     CDS             12834..13838
                     /locus_tag="VF097_09325"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX6263033.1"
                     /translation="MAGRRTNLALLALLALALATGGLAFAIGTTWNRWIVLAHGVAGL
                     ALLALAPWKSAVARRGLRRARRDSWASVLLTVLVAVAILFGVLHAVGIVSVGPLTSMQ
                     LHVGAALGSVPFAIWHVVARSVRPRRTDLTRRNLVRAGAVVGAGGLAYAAIEGSVRIA
                     SLPGRDRRFTGSHETGSFDPEAMPVTQWLNDSVPEIDADSWRLVVRAGDREDAWSYEE
                     LTAFGDRLRATLDCTGGWFAVQDWEGVRLSRLLPEGEEGRSIHVASATGYGRRFPLQD
                     ADHLLLAMRVGAEPLSAGHGFPARIVAPGRRGFWWVKWVTRIELSPIPWWWQPPFPLA
                     "
     gene            13871..15076
                     /locus_tag="VF097_09330"
     CDS             13871..15076
                     /locus_tag="VF097_09330"
                     /inference="COORDINATES: protein motif:HMM:NF028011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6263034.1"
                     /translation="MDDLTKAELSQRSGKPPEYIDRLVELGILTPLSDGSFRSADIQR
                     VKLIDALVRSGIRLEDLSRAVRSGNLSFAFVDLLFTEPKGYLPKTYEDACTEYGWTMD
                     FVQRAYEVLGLPVPSGQDPVREDDMQMFPIGRFTLAAGIPEAGVLRSLRVYAENLARI
                     TRAESQFFHTYIEEPLLAGGAGEAQILEVSSQLSPQLRGQVEGMIWWLYRRYQEHSII
                     EHLVGHVEEALDQAGVSPRREVHPPAIVFVDLVGYTRLTEERGDEAAAELAAALAGLV
                     QGESQRRGGRPVKWLGDGVMIHFPDPGQAVLCSLELVEKAPEAGMPPAHIGVSAGSVV
                     FRDGDYFGRTVNLASRIAGRAGAGEVLVSEAASRSASVEGVRFETMDPVELKGMAEPI
                     QVFRAVRTS"
     gene            complement(15131..15376)
                     /locus_tag="VF097_09335"
     CDS             complement(15131..15376)
                     /locus_tag="VF097_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX6263035.1"
                     /translation="MMMGDGWGGWIWGALMMLLFWGGLIALVVFLVRGSPSRTSHGEE
                     RRRGPEARDILAERFARGEISEDEFEQRRRVLERSSS"
     gene            complement(15382..15624)
                     /locus_tag="VF097_09340"
     CDS             complement(15382..15624)
                     /locus_tag="VF097_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263036.1"
                     /translation="MKKFLIGAAMLLTVVAALRRFGPALGRLAMRKCEEMLARMPEDF
                     PPKRMMHGIEEIRAQNARILHHLEEGTSSTAALGRR"
     gene            15858..>16433
                     /locus_tag="VF097_09345"
     CDS             15858..>16433
                     /locus_tag="VF097_09345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6263037.1"
                     /translation="MDSSRHTERIAIVTGAGSGIGRATVLRLAQEGARVVGCDVVEPA
                     LAETKEILDQAGLSAELVSGDVTRQEDVDRIVDAAGERVDVLANVAGIMDHFLPLGEV
                     DDETWDRVLAVNLTGVMRLSRAVLPSMLEAGGGSIVTVASKASLGAGASGVAYAASKH
                     GVIGLVKSIAYFYGPRGIRSNAVLPGPVETGI"
BASE COUNT         2267 a         6196 c         5604 g         2366 t
ORIGIN      
        1 cgaggcccag cagcgcggcc atgcgcgcca cggcgcggcc gtggttgccg tcggtggccg
       61 ccgcgagggt aaggggccgg agcggggtga ggcgctccga gagctccgcg acgtccgacc
      121 acggctccac gtcgcggccc agccgctcca cgagcgtgcg atacgtggcc caggacgcgc
      181 cgaggatctt gaacgacggg aggccgagac gacgggactc gtccttcacc cacacggtcc
      241 cgaccccgag acgccgggcc accgtggggg cctccaccac gggcgtcacc tcgtagccgg
      301 gcagccgccg gtggaacgcc agggggtcgg ggcccgtgga cggcgccggg acggttcccc
      361 ggagagggtt cgggaggatc cgatcggagg aggacatcat ccgcagctca cgccgcgccg
      421 aacccgccca tcgtgagggc catcagggcc ttcccggtgt ggaggcggtt ctcggcctcc
      481 tcgtagacga ccgaccgcgg gccatcgatc acctcgtcgg tcacctcgtt cccccgatcg
      541 gcggggaggc agtgcatgta gagggcgtcg tccttggcca gctccatccg ccgcgcgtcg
      601 cagatccagc cgcggtacgg ctccgaggcg gcgagcgcct cctcctcctg ggtgaagagg
      661 tcgagccgcc cccaggactt cgggtagacg gcatcggctc cctcgaaagc ctcgtccatg
      721 tcgttcgtca ccgtgatcga atcggcaccc tcggtcgcag ccttctcggc ccgcgccatc
      781 agctcgggca tgagctccca gccgggaggg tgcgccagca cgacgtcgac cccgaaccga
      841 ggcaggagcg cggccagccc ctggggcacc gagagcggct tggcgtacga aggcgcgtag
      901 gcccacgaaa cggcgacccg catcccccgc agatcggccc cgcgcttcgc ccggagggtc
      961 atgaggtcgg ccagggtctg cgtcgggtgg tccacgtcgc actggagatt gagcaccggc
     1021 acctccgccc accgggccac ctctcggatg tacgcgttgc actcgtaggg gatgaggtcg
     1081 tgacggatcg cgatcccgtg cccgtacgac gagaggatga tccccatgtc cttcgggctc
     1141 tcgccgtggg agacctgcgt cgcctcggcg gtgaggtagt gcgcgtgccc ccccagctgg
     1201 gtcatcccgg cctcgaacgc gttccgggtg cgcgtggact tgtcgaggaa gagcatgaac
     1261 agcgtcttgt cggcgagcag tcggtggggc tcgcctcgct tgaaccgctc cttcagatcg
     1321 gccgagacgg cgagtgcttc ctcgagctcc tccaccgacc agtccagcgt ctcgatgtag
     1381 tcccgccccg tcagggcttc gcgttccacg cctcctccct cctcaggcct tctgggcgag
     1441 ctcggtggcc ggcacccccg cgtacacggg gaccggctcc tggccgggga cgtccgggac
     1501 gcgcggccac ccgcgcgtgg agacgggggg cgcgatcccg accaggtcgt ccggacgcac
     1561 cggcacccgg ttcacctccc tcccggtggc gtcccggagc gcggcggcga cggccgcggt
     1621 ggccacgatc gtggagggct cgcccacgcc cttggcgccg aacggcacgc cgggctcggg
     1681 ctcctcgacc agctcggaga ccaccggggg catgtccagc gtcgtgggga tcaggtagtc
     1741 ggtgaacgag gcgttcagga tccgaccctc ctccagctgg atctcctcca tcagcgcgag
     1801 gccgaggccc tgggcgatgc cgccctcgat ctgcccacga acgctctgcg ggttgagcgc
     1861 ccgaccgacg tcctgggccg ccgccacctc gaccacgcgc acgaggccca ggtcctcgtc
     1921 cacctcgacc accgcccgcg cggcggcgaa ggcgaacgag acgtggaagt cggcctgacc
     1981 gtcgggatcg aacggctcgg tcggacggtg gcggtagacg ctcgactcct cgatcggatc
     2041 ggtgaggaac tccgcgaggt cgcccacggg aagcccctcc gccagcaccc tcccttcctc
     2101 cagggtgagc ggggcgtccc ccacctccgg tcgagcccgg cgcacccgct cgaagagctc
     2161 ttcccggacg gcccggcatg cgagctgcac ggcccctccc gacatcatcg tctgccgaga
     2221 ggccgagctc gaccccgcgg atgcgacgcc cgtgtcgggg gtgagcagct cgacgcgctc
     2281 gaccccgagc tcggtcccgg cgatctgcgc caggatcgtg tgcacgccct ggcccacctc
     2341 cgcggcggcg ctgtgcacct ccgccaccgg accgtccggc cccgccgaca gcctgacccg
     2401 cgccgccgcg gagtcgtcga accctccgct gtaggcgatg ttcttgtagc cgacggcgaa
     2461 ccccacgccg cgcctcaggc cttctccccg acccacgttg ttggccccgc cggggagcgt
     2521 gacgggctcc cgcatgcctt cgggctcggg gggcatcggg atccgggcgc agcgctcgat
     2581 cacctcgcgg accggggccg acccccggat cacctggccc gtggggagca gcgacccggt
     2641 cgagagcgcg ttgcgctcgc gcagctcgac ggggtccatc gccagggccg cagccagccg
     2701 gtccatctgc gactcgtagc cgacggccgc ctggacggcg ccgaagcccc gcatcgcgcc
     2761 gcagggcggg ttgttcgtgt agacgcaggt cccctcgagg agggcgttcg ggacctcgta
     2821 gggccccgcc gcgaacgtcg tcgcgttcgc gatgacggcc gccgacgacg aggagtaggc
     2881 gcctccgtcg atgacgatcc tcgcccggac gctcaccagc cgcccgtcgc tcgtggcgcc
     2941 gtggcgcatc cagatgcggg ccgggtggcg gtgcacgtgg ccgaagaagg actcctcacg
     3001 cccgtaggac atcttgacgg gcctcccggt gcggagagcg agcatgctcg cgtggatctg
     3061 catgctcacg tcctcccggg ccccgaaggc gcccccaacc cccgccaggt gcaggcggag
     3121 cttctcatcg ggaaggccga ggcacccggc gatctgccgg tggtcgacgt ggagccactg
     3181 ggtggccacg tacagatcga cccctccgtc ctccgagggg acggccagcc cggcctccgg
     3241 gccgaggggc gcctgatcct gcatccccgt ctcgtagtag ccctccaccc agacgtcggc
     3301 ctcggcctcc gggtcgccgt gtgcgatccg gacgtgtcgc aggacgttgc cccattcgtg
     3361 cagcttcggc gcgtcgggct ccatggccag ctccatgtcg tccacgacgg ggagctcctc
     3421 gtactcgacc cggatgagtg cggccgccct gcgcgcgagc tccgggtcct cggcggccac
     3481 ggcggccacc ggctccccct ggtagcgcac tcgatcgatc gccagcaccg gctggtcctg
     3541 gaactccagc ccgtacatct tgtggcccgg cacgtcctcg gaagtcagga ccgcgtgcac
     3601 accggccgcg gccaccgccc gggagacgtc gatcgagcgg acacgggcat ggggatgcgg
     3661 ggtccggagc gtgtggcccc acagcatgcc ctcggcccac aggtcgctgc cgaactcgaa
     3721 ctcccctcgg accttcggga cgccgtcgac ccgacgcacg ctctccccga cgccccggac
     3781 cagccgctcc ccgatccgaa cgctcatgcc cgacctccca gagctcgcgc cgatgcccgc
     3841 cgcccccggc gagccacctc ctcctcgtcc accccggtga ggcgtccgtc ctcgacgacg
     3901 gcgtggccgt ccaccagcag atcccgaacg cggccgggcg agcagaagag cagcgccgcc
     3961 accggatccg acccggcgcc cgcgaacccc aacccttcga ccgagaagag agccacgtcg
     4021 ccgcgcttgc cgacctccag cgacccgacg tcgtcccggc ccagggccgc cgccccgccg
     4081 cgcgttgcca cgcggagggc ctcgcgagca ctcatggcgg cggggccgtc ccccccacgc
     4141 gcggtcagca tcgcctggcg aacctcgccc agcaggtggc cggcgtcgtt ggaggcggac
     4201 ccgtccaccc cgagacccac gttcgccccc tcgtccagga gcgagcgggc cggcgcggtc
     4261 ccggacccga gtcgcaggtt cgaggacggg caccacgcgg tcgaggtgcc ggactcggcc
     4321 atcctgcgga cgtccttctg gtcgaggtgc acgcagtgcg cgagccagac gtcggggccg
     4381 aggaagccga ggtcggccag gagctccagc ggccgccggc cgaaccgctc gaggcagaat
     4441 cgctcctcgt cccgggtctc cgcgatgtgg gtgtggagcc ggacgtcgtg ccgacgggcg
     4501 agctccgccg actcccgcat cagccgctcg gtcgaggaga acggggagca gggggccacc
     4561 gagatcctca gcatggctcc cggggcgggg tcgtggaagc gggcgatcgc ctcctcggtc
     4621 tcttgcagga cctggtcggt gtcctccacc acccggtcgg gggggaggcc gccgtccgac
     4681 tctccgaggt ccatcgaccc ccggcacggg tggaaccgga gcccgacctc gcgggccgcc
     4741 tcgatctcgg cttccaggag cccgtcggcc ccctccggga agatgtagtg gtggtcggtg
     4801 gtcgtcgcgc agccgctcag ggcgagctcg gccaggccaa cccgggccgc ggccagctcc
     4861 cattctgcgt ccagccccgc ccagatcggg tagagctcga ccagccatcc gaagagcccc
     4921 tcgtcctggg cccggacccg ggtgagcgtc tggaagaggt gatgatgggt gttgatgagg
     4981 ccggggatcg ccacggcccc gcgcccgtcg acgcgccggg ccccctcagc gtcgggaggc
     5041 tccccccacc ccacccaggc gatgacgccg tcctcgatca ggagggagcc gcccgggatc
     5101 tcggtgccgg cgtcgtccat cgtgaccagc acctcgcagc cctccaccag gagcctcgtc
     5161 acgcggtgcc ccccatccgc tcggccgcca ggcgcaccgc gtccaggatc ttcgcgtagc
     5221 ccgtgcaccg gcagaggttc cccgagaggg cctcgcggac ggtggcctcg ctcggggcgg
     5281 gatcccgctg aagcaggtcg gtgacggcca cgatcaggcc gggggtgcag aaaccgcact
     5341 gcacggcgcc cgtctccagg aacgcctcct ggacggggtg gaggaggtcg tccgatccca
     5401 gcccctcgac cgtccgcacc tcgcgctcgt gcgcctgggc tgcgagcacg aggcacgagc
     5461 acacgagctc gccgtccagc cagaccgagc aggagccgca ctccccctgc tcgcacgcgt
     5521 tcttcgagcc gggaagccct agatcttccc ggaggaagga gaggaggctg gaccctgccc
     5581 aggcgtccgc ctcgcgccac tccccgttca cgttcgtcct gatcagcacc tcgcctcctc
     5641 acgcagcccc ggtcgctcgc tcccccaggc tccaggtgag cgcccgccga gcgagcacgc
     5701 ggcaggcgtg gcggcggtag gccgccgtgc cccgcacgtc gtcgatcggt cgagccgccc
     5761 gcgccacgag gtcggcgacg tcgtcgagga ccccgtccgg gagggcagcc gccggcgact
     5821 cccaggcgcc cgcctcggcg atcgcccggg ccgcgagcgc ctccgcctcc gccgcccgga
     5881 tgatcgtggg ccccaccgag cccagcgcga accgcacggc gcgatcgtcc tcgtcgagca
     5941 cgaggcagag cccgaccgcc gagatcacca tcgcgttgcg gaccccgatc ttcgagaagc
     6001 tccccgggcc gcgcggagga cggaaccgca cccccaggat cagctcctcc gggcccagcg
     6061 cggtctgctt cgggcctcgg aggaactcgc tccacgggag ggagcgcgga tcggccgctt
     6121 ccctccccac cacgatctcg gcgtcgtagg cggccaggac gggcagcgcg tcgcctgccg
     6181 gcgaggcggt gcccaggttc cccccgatcg tgccgcggtt gcggatctgc ggggagccca
     6241 ccgtgcgcga cgcctggaac agcgggacga gctcggggag ctcgcgcatg atccgcgtga
     6301 aggtgacgcc ggctccgagg aagatcctcc cgtcctctcg gccgtgctcc cgcagctccg
     6361 gcaccggcca gaggtccagg atggcctcgg gccggtcccg gtcgaagttc agctcgacca
     6421 tgaggtctgt cccccccgcg atggggcggg cctccgggtg cgccgccttc atcgccaggg
     6481 cctcctggag gccgcgcggc cgcaggacct ccacggttag aacgctccct ccgcgaaggc
     6541 gtcgagcatc gcccggacat cgtcccccag ggggtagagc acggggcacg tgcagccggc
     6601 ctccacgtac tcccggacct tggcccggca ctcctcggcc gtgcccgagg ccgtgatcat
     6661 ctgcaccacg tcgtcgggca cgaggcgcat cgcgttctcg atctcctcag gaccggcggg
     6721 ccacgtgagg acctctgcga tctcatcgag gagcgactgg tccaccccgc tggccttcat
     6781 gatgtggggc tgctgtccca ggtactgggt gacgagctct cgcgcgcggt ccagcgcggc
     6841 ctcgcgatcc tcgtcgaggg agcacacgac gagctgcggg cggtccacgt cctccacgct
     6901 cctccccgcc cgctcggcgc ccgccgccag agctcccatc gcggcgcgat cgtactccgg
     6961 gctcacgagg tagttcagga ggacgccgtc ggcgatctcc ccggccagct ccatcatccg
     7021 catcccggtg gcgccgatgt agatcgggac gtccttgggg gagcggtcgc cgtgcacgat
     7081 gtcgatctcg atgtcctcga cttcgacgaa ctcgccgtgg aaggtgactc gctccatggc
     7141 cagaagccgc ctggtgacct ccaccacctc tcgcatcgcg gtcaggggct tccgccgcct
     7201 gacccccacc ttcgaggcca gcggctccca ccacgccccg atcccgagca tgatccggcc
     7261 gggcgccagc tcgtcgagcg tggcgaacgt ggcggccagg agccccacgt tgcgcgtcca
     7321 gctgttcact accccggagc ccaccttgat gcggtccgtg acggcggcga aggcggccat
     7381 cggcacggtg gcctcgcgga cgaggcggct ctccgcctgc cacacggctt cgaagccgcg
     7441 ctcctcggcg tccctcacgt accgcatccc gtcccggatc gggtgcttgt cctgcaggta
     7501 gagcgcgacg cgcccctcgc tggagaccgc catcaggccg cccccgacgg ggagaacggg
     7561 tgctcggcga tctcgtccat ccgccgccac agccgctcgg ccgactcgcg ggcccgggcc
     7621 gcgacctcgc tctggtccac gcgggtcagc ctcccgtctc ggaccacggg ctcgccctcc
     7681 acgacgacgt cccgcacctg ccgggccgag aggccgaaga cccagtgccc ggcgagggtc
     7741 tccgcgtcga gcggggtcgg cgggtcgtac gccaggacca ccaggtcggc gggggcgcct
     7801 cgctcgatcg tgcccagcag cggctcgtcg aagatcaagc ccgcgaaccg ggccgatcga
     7861 gcgaggcgcc ccaggaccca cgacggcgcc gacgcggtcc ccgcctcccg tgcccgccag
     7921 tacgccgctc ggccctcggc gaacatgtcg cccccgatcc cgtcggtccc cagggccagg
     7981 cgatcgccaa ggagggccac tggggcgtgg ccgacggcgt tgttcatgtt cgacctgggg
     8041 ttgtgcaccg gggtcgcccc cgtctcgcga atcacctccg cctcgtccgg cccgacgtgg
     8101 atgcagtgag ccaggagcgc ccgctcgtcc agggccccgg cctcggccag gcggtggacg
     8161 acccggcgac cgaaccgagc ccgggcgtcg tcctggtcgg ccccgtcctc cgccacgtgg
     8221 atgtggatcc cggctcccag ctcacgggcc gcctccacgc agccggccag ggtgtcctcg
     8281 gagagcgtga aggaggcgtg ggcccccacc atcccccggg ccaagggcga gccgccgccc
     8341 ttcaggaacc ggcggttctc ctcgatgccg gcctccgcct tctccgggcc gtcccggtcg
     8401 gtgacctcgt agcagagcac cgagcggatc cccaggtcgc ccagggcgga ggccaggcga
     8461 tccagggatc cctcgatgtc gcccggagac gcgtggtggt cgatgacggt ggtggtcccc
     8521 gagaggagcg cctccgaacc gcccaccgcg gccgaccacc gcaccccctg gggatcgagg
     8581 gcccggtcga gacgccacca gacccgctgc aggatctgga cgaagctctc gggcggttcg
     8641 agcgcgtacg gcatcccccg ggccagcgac gagtagaggt ggtggtgggc gcagacgttc
     8701 ccggggatca ccaagcaccc cgagcagtcg agcgtcgcgt cgccttctcc ggatccgagc
     8761 tcggccaccc tcccgtccgc gatccggacg tcggcggcct cgacgatcgg cgggtcgagc
     8821 gagcgcacca ccgtgccccc ggtgagcacg aggcccgcgc cgcccgtcac ctcacccctc
     8881 tcgatccgcg ctccggcgtg ccgcggcggc cagcttgggg acggcggccc tcgccgccgg
     8941 ggagtccttc atcgggagcc tggtccgccg aaccccgtcg aaggcgtgca gcgcgccggc
     9001 caccgccgcc gccgtgggca cgagcccgat ctcccccacg cccttggcgc cgtagggacc
     9061 ttcgggctga tgctcctcca cgaagatgca ctcgaccggg ggcatcccgg ccggagggat
     9121 gatgtgcagc gacttcagcg tgtcggtgac ggggaccccg ccctccagaa cgagctcctc
     9181 ggagagggcg tgcccgagcc ccatgtgcac cgccccctcg atctgcccct cgaggagggt
     9241 ggggttgatc accttcccca cgtcgtgggc ggcgacgacc ttctcgatgc ggccctcccc
     9301 gtcgagggtc acgacctgcg tcgcccatcc gtacgcgtag tgggtgaccg ggttctccac
     9361 ccccagcttc gtcgtccagt ccacgacgaa ctcgccgtag aactcgcggc ccgccaggtc
     9421 ctcgaggcgg cccccgttca actcggcctt gaggttccgg gcggcctcga tcacggcccg
     9481 gccgcccagg acggtggacc gcgacgccgt gcactgcccc gtgtcgatgt cccggatcgt
     9541 gtccaccgcg acccggatcc ggtcggaggt gatgccgagc tcctcacacg cgatctgctg
     9601 gagggcggtg tggacaccct gtcccatctc ggtccacgag tggaacagcg tgacgctccc
     9661 gtcgtcctcc acccgcagga cggccttccc gtactcggtg aggccgttcc ccaccccgct
     9721 gttcttcagc ccgcaggcga tcccggcgcg cggggccgcc cggtagacgt cgcgcaccgc
     9781 gagcagggtc tgcttgatcc ccacccccgg gccgagcacc tgcccggtgg cgaacacgtc
     9841 gccggtctcg agggcgttgc gccatcggat ctcccagccg tcgatcccca cccgctccgc
     9901 gagcgtgtcc agcacaccct cgatcgcgaa gttgacctgg ttcacgccga accctcgcat
     9961 cgcccccgca ggcgggttgt tggtgtagac ggctcgggcc tccacgtcca cggcgggcac
    10021 gcggtagggg ccgcaggcgt gcccggccgc ccgctccagg accttgtccc ccacgctcgc
    10081 gtaggcgccg gtgtcgccca cgatgcgggc ccggatcccg aggaggcgcc cctcctggtc
    10141 gcacccgacc gtgtactcca tcgtcagcgg gtggcgcttg gcgtggaagc ggatgctctc
    10201 ctcgcgggat agcgtgacga ggaccgggcg cccggtcatc agggccaaga ggcaggcgtg
    10261 cccctgaacg ttgagatcct ccttggcccc gaaggcgccc ccgttcgaca cctgcgtgac
    10321 gcggatccgc tcctcgggca tcgccaggag gctggccacc tgccggcggt cgtcccacac
    10381 cccctggccc tgggagtaga agtgcaggcc gccgtcctcg gtcggcacgg cgagcgacga
    10441 ctcgggctcg aggaaggcgt gctcgaccca ctgggtccgg taggtctcgg tgacgacgtg
    10501 cgcggcctcc cccagcgcgg cgtccacgtc gccccgcttc acctccgaga tcgagagcac
    10561 gttgccgccc tcgtgcagct ggggggcgtc ctcggccatg gcctcgaagg ggtcggtgac
    10621 cggcgggagc acgtcgtact cgacgtccac gagggcggcc gcctctcggg cctcggcccg
    10681 agaggcggcc gccaccaccg ccaggacgtc gccgacgcag ctcacctcct cgccctcggc
    10741 cacgagctgg cgccagtcct tcgtgatgag cccctgtcgc cgctcccccg ggacgtcgtc
    10801 ggccgtggcc accgccacca cccccgggtg ggcctcggcc ctcgaggtgt cgatccgaag
    10861 cacacgggcc ctagggtggt cggagaaccg gatcgctcca tggagcatgc ccggcacgcg
    10921 catgtcgttg acgaagggct tctcgccgag cgccagctcg cgtccctcgt accggccggt
    10981 ccgggagccc acgcggccgc tgcggtcaac ctccgggagc ggctcgcctc ttcgcgttcc
    11041 ggccgcgtgc tcgatcgcat cgatgatctt cacgtagccg gtgcaccggc agaggttccc
    11101 cgcgatggcc cgggcgacct cctcccgcga gggctcgggg ttcttcgcca gcatcgcctc
    11161 gcacttcatg accaggcccg gggagcagaa cccgcactgc gacgccccgg aggccaagaa
    11221 gcactcggcc cactcgtcgc gccgctcctg cgggaggccc tcgagggtgg tgacctcctt
    11281 gccggcgacc cgggtggctc gctgagcgca ggagacgacc cccttcccgt ccaccatcac
    11341 ggtgcacgcg ccgcaggatc cctcgggcgc gcacccgtcc ttcatcgagg tgagcccgag
    11401 gccctccctg agcaccgaga gcagcatcgt gccctcgtcg acctccacgg ataccggccg
    11461 gccgttgagg acgaactcga ggggggcagc ttcggacacg gacgtcacct ccggtcccat
    11521 cctctcagga ggaacccgtc gcggccaggt agctctccgg gaaggccccg taccaggcga
    11581 cggccggggc caggtgatcc agcgggcact ggtcgtccgc gctgtgcgcg tacttctcct
    11641 cggcggggcc gaagccgatc gtcgtgatcc cgagcttccc catgctcgag accccgttcg
    11701 tggagaagat ccatcgaccc acggccggcg tcctcccctg ggtgcgctcg cccgcctcga
    11761 ctccggcccg gacggccggg tcgtcctcgg gcaccaccca cgtggggaag tacttctcgg
    11821 tgctgagcgt gaggcccgtg tagctctcgc cctcgtactg gaggacctcg acctcggctc
    11881 cggcctcctg cactgcggag agctcctcca gctctttccg ggcggtctcc agagtctccc
    11941 ctacggtgag ccggcggtcc atgtacacgc tgcagccgtc gggcacggcg ttgagcgagg
    12001 gcgtccggca gtcgatcttg gagaggacga tggtcccctt ccccaggaac ggatcgtcgg
    12061 ccaggcgctc gttcagctcc tcgatctgcc ggatgatcgg ggccatccgg tagatcgcgt
    12121 tgatccctcg ctcgggggcg gaggcgtgcg ccgcccgacc gcgcgtgtgg acggcgagct
    12181 cgacgcgccc acggtgcccc cggtacacgt cgagctccgt ggcctcgccc agcacgacga
    12241 catcgggcct ggggatgctc tcggtcagca cgtactcgag ggcgagcccg tcgcagtcct
    12301 cctcctgcac cgtccccacg acgtagacgg tgaagcgaga cgagtcgatc cccagacgct
    12361 tgatcagcgc ccccccgtag accatcgtgg ccgtggcggc cttgttgtcg gaggcgccgc
    12421 gcccgtagat cacgccgtcc tcgatgcggg gggtgaacgg gtccgccttc cacgagctcc
    12481 gctcccccac gtccaccgtg tcgatgtgcg agtcgtagac gattcccacc ggcccgtctc
    12541 cgatcctccc gatgacgctg ccgaagccgt cgatcgtgac ctcgtcgtag cccaggtcgg
    12601 tcatctcgtc gccgatgcgc cgagcgacgt ccccctcacg acgggagagg ctggggatcg
    12661 cgatgatgtc cgagaggaac gcggtcgttc gctcggcgag cgcgcccgcc tcgtcctgga
    12721 atcgttcctc gagcctcata tctctcctct ccccgccggg ccgcggttcg acgcagggtc
    12781 gcttgaagtg aagcacaact ccggagtgcg ggggtcccgg gtagactcga ccgatggcgg
    12841 gacggcgcac gaatctcgcc ctcctcgccc tgctggccct cgcgctcgcc accgggggtc
    12901 tcgcgttcgc gatcggaacg acgtggaacc ggtggatcgt cctagcgcac ggcgtggcgg
    12961 ggctggcgct cctggccctc gccccctgga agtccgccgt cgcccgccga ggcctccggc
    13021 gggctcgccg cgattcctgg gcctcggtgc tcctgacggt gctcgtggcc gtcgccatcc
    13081 tgttcggcgt cctccacgcc gtgggcatcg tgtcggtggg gcctctcact tcgatgcagc
    13141 tgcacgtggg agccgcgctc ggctcggtcc ccttcgccat ctggcacgtc gtggcccgga
    13201 gcgtgcgccc gaggaggacg gacctcaccc gccgcaacct ggttcgggcg ggagccgtcg
    13261 tgggagcggg cgggctcgcc tacgcggcga tcgagggaag cgtgcggatc gcctcgctcc
    13321 ccgggcggga tcggcgcttc accggctcgc acgagaccgg ctccttcgac ccggaggcga
    13381 tgcccgtgac ccagtggctc aacgactcgg tccccgagat cgacgccgac agctggcgcc
    13441 tggtcgtgcg ggcgggagac cgagaggacg cctggtccta cgaggagctg accgccttcg
    13501 gcgatcgact gcgggcgacc ctggactgca ccggcggctg gttcgcggtg caggactggg
    13561 agggggtccg gctctcgcgg ctcctccccg aaggggagga ggggcgcagc atccacgtgg
    13621 cgtccgcgac gggctacggc cgccggttcc ctctccagga cgccgaccac ctgctgctgg
    13681 cgatgcgggt cggagcggag ccgctcagcg ccggtcacgg gtttcccgct cggatcgtcg
    13741 cgccggggcg gcgcggcttc tggtgggtga agtgggtcac gcgcatcgag ctcagcccga
    13801 tcccttggtg gtggcagccc cccttcccgc tcgcctgagc tccgcgagac atctcggtac
    13861 gatgccttcg ttggatgacc tgacgaaggc cgagctgtca caacgatcgg gcaagccacc
    13921 cgagtacatc gatcgcctgg tcgagctggg gatcctcacg cccctgtcgg acgggtcatt
    13981 ccgatccgcc gacatccagc gggtcaagct catcgatgcc ctggtgcgat cagggatccg
    14041 gctcgaggac ctgagccgcg ccgtccgctc gggcaacctc tccttcgcgt tcgtcgacct
    14101 gctgttcacc gagccgaagg ggtacttgcc gaagacctac gaggacgcgt gtacggagta
    14161 cggctggacg atggacttcg tccagcgtgc ctacgaggtc cttggcctgc cggtcccctc
    14221 gggtcaagac cccgtccgcg aggacgacat gcagatgttc ccgatcggcc ggttcaccct
    14281 ggcggccggc atcccggaag caggggtcct ccggtcgctc cgcgtgtacg ccgagaatct
    14341 cgccaggatc acccgggcag agtcccagtt cttccacacg tacatcgagg aaccgctgct
    14401 tgccggagga gccggcgagg cccagatcct cgaggtgtcc tcccagctga gcccccagct
    14461 gcgcggtcag gtcgagggga tgatctggtg gctctaccgc cgataccagg agcactccat
    14521 catcgagcac ctggtcggcc acgtcgaaga ggcgctcgat caggccggtg tgagcccacg
    14581 cagagaggtc cacccgccgg cgatcgtctt cgtcgatctc gtgggctaca cgcgcctcac
    14641 cgaggagcgg ggggacgagg ctgcggccga gctcgccgcg gcgctcgcgg ggctggtgca
    14701 aggcgagtcc cagcggcgcg gagggcgacc ggtcaagtgg ctcggcgacg gcgtgatgat
    14761 ccacttcccc gacccgggcc aagccgtcct gtgctccctc gaactggtcg agaaggcgcc
    14821 ggaggccggg atgcctccag cgcatatcgg cgtcagcgcc gggtcggtcg tcttcaggga
    14881 cggcgactac ttcggacgaa cggtcaacct ggcgtcccgg atcgccggga gagcaggggc
    14941 gggtgaggtg ctggtgagcg aggccgcgag tcgctcagcc tcggtcgaag gggttcgctt
    15001 cgagaccatg gaccccgtgg agctgaaggg catggccgaa ccgatccagg tgttccgggc
    15061 ggtccgaact tcgtaggcgc cctcgaaggc gcactcgacc aggtccctac acgaggccct
    15121 gctcatcgcc tcaggacgag ctgcgctcga gcactcgcct ccgctgctcg aactcgtcct
    15181 cggagatctc ccctcgcgcg aagcgttccg ccaggatgtc ccgggcttcc ggccctctcc
    15241 gcctctcctc tccatgagag gtgcgggaag gggagccgcg aaccaagaag accaccaggg
    15301 cgatgaggcc accccagaac agcagcatca tcagggcgcc ccagatccag cctccccaac
    15361 cgtctcccat catcatcgtc ctcacctccg tcccagagcg gcggtcgacg acgtcccttc
    15421 ctcgaggtga tgcaggatcc gtgcgttctg cgcacggatc tcctcgatgc cgtgcatcat
    15481 ccgcttcggt gggaaatcct ccggcatgcg agcgagcatc tcctcgcact tccgcatggc
    15541 aagcctgccc agcgccggcc cgaagcgtcg gagcgccgcc acgaccgtca ggagcatcgc
    15601 ggcaccgatc aggaacttct tcatcggtcc ctcctcaccg tcaccggtag cacgaaaccg
    15661 ccggtggcac gcgtccaagt caacgtgtgc ctgtcgcctt cagtcgtaca gggccgaagg
    15721 tcccggggca gacgggaccg tgcggtatgg gatgaagtcg cctgaggggc gacgacggtc
    15781 ccctttgggc aggacctggg gcaggattga gctcggagga gcgcccaccg acgggcagtc
    15841 gagaccgagg aggagccatg gacagctcgc gtcacacgga gaggatcgcg atcgtgaccg
    15901 gcgccgggag cgggatcggc cgcgcgacgg tgctccggct cgcccaggag ggagcccggg
    15961 tggtcggctg cgacgtcgtc gagcccgccc tcgccgagac gaaggagatc ctggaccagg
    16021 caggcctctc cgccgagctc gtaagcggcg acgtcacccg gcaggaggac gtcgatcgga
    16081 tcgtcgacgc cgcgggcgag cgcgtcgacg tcttggccaa cgtcgccggc atcatggacc
    16141 acttcctccc gctcggcgag gtcgacgacg agacgtggga tcgcgtcctc gccgtcaacc
    16201 tcacgggcgt catgcgcctg tcccgcgcgg tgctcccctc gatgctggag gcgggcgggg
    16261 gctcgatcgt gacggtcgcc tcgaaggcct ctctcggggc gggggcgtcc ggcgtcgcgt
    16321 acgcggcctc caagcacgga gtgatcggcc tcgtcaagag catcgcctac ttctacgggc
    16381 cccggggcat ccgctcgaac gccgtcctcc ccggtccggt ggagacgggc atc
//
LOCUS       DASUTZ010000216         2308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1241529, whole genome shotgun sequence.
ACCESSION   DASUTZ010000216 DASUTZ010000000
VERSION     DASUTZ010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241529"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /gene="selA"
                     /locus_tag="VF097_09350"
     CDS             complement(<1..1041)
                     /gene="selA"
                     /locus_tag="VF097_09350"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEX6263038.1"
                     /translation="MVDQRRRLPSVDALLRSEPGVRASERFGRDVVRAAVREVLDETR
                     RGTGRRRAMPDEEALMARAVHRAAAASLGLSGVVNATGVVLHTGLGRAPLPEEAVEAV
                     ARSARGYADLEVDRETGERGSRGARAEFLLRAITGAEAALVLNNNAAALVLVLSALAG
                     RREVLVSRGELIEIGGEFRLPDIMRASGAKLVEVGTTNRTRVADYERALGGRTAMILK
                     VHPSNYRVVGFSDSPEPRPLAALAERAGVPLVYDVGSGLLRRQRGVPLDEPSVDEALR
                     AADLVCFSGDKLLGGPQAGVIVGRAELVERLRKHPLARALRIDKMTVAAMEAGLRMHA
                     EGRRGELPLWRQL"
     gene            complement(1088..1393)
                     /locus_tag="VF097_09355"
     CDS             complement(1088..1393)
                     /locus_tag="VF097_09355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01926.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HEX6263039.1"
                     /translation="MTHHRRGLRRLLKDDELLARVERDWRGAGLDPKRIAMLEYAEKL
                     TLRPADVSREDVDALREAGFSDEDVLHICEVVAYYAYANRIADGLGVELEPWIPESD"
     gene            complement(1394..1642)
                     /locus_tag="VF097_09360"
     CDS             complement(1394..1642)
                     /locus_tag="VF097_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX6263040.1"
                     /translation="MAWIEVRGDDEAEGRLAELYARMVDPTYGRVDMIMQIHSLHPDG
                     MRTHDELYREVMKSTKTLRKVERELIALVVSQVNECHY"
     gene            1715..>2308
                     /locus_tag="VF097_09365"
     CDS             1715..>2308
                     /locus_tag="VF097_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020522161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263041.1"
                     /translation="MDTPVNRASRERFVTLPIMEEKGFVELRRYESPPEPSEWEDLEY
                     VDWKSGGDTNFAILASANGDHDPRGFWEHGKPDKDGVWVEDNIEKSPTIKKWVESVGA
                     NFGRVRIIKLEPNTYEDTVKNMHPDDNNRITEDGTGYVVRAWLQLTDNPESYMIVREE
                     KDDEASESRIPLPRGAQFVVDSERLWHAVWHNDEHYSE"
BASE COUNT          367 a          861 c          767 g          313 t
ORIGIN      
        1 gagctgccgc cacagcggca gctcgccgcg acgcccctcc gcgtgcatcc gcagtccggc
       61 ctccatcgcg gcgacggtca tcttgtcgat ccgcaaggcc ctggccaggg ggtgcttccg
      121 gaggcgctcc acgagctcgg ctctccctac gatcacgccc gcctgaggtc ctcccagcag
      181 cttgtccccc gagaagcaga ccaggtcggc cgcgcggagg gcctcgtcga cggacggctc
      241 gtcgagcggc acccctcgct gccggcggag gagccccgac cccacgtcgt agacgagcgg
      301 aacaccggcc cgctccgcca gggccgcgag cggccgaggc tccgggctgt cgctgaatcc
      361 cacgacccgg tagttgctcg gatgaacctt gaggatcatc gcggtccggc cgcccagggc
      421 ccgctcgtaa tccgcgaccc tcgtgcggtt cgtggtcccg acctcgacga gcttggcccc
      481 cgaggccctc atgatgtccg ggagccggaa ctcgcccccg atctcgatga gctcccccct
      541 cgagacgagc acctcgcgcc gcccggccag ggcggagagc accagcacca gggccgccgc
      601 gttgttgttg agcacgaggg cggcctccgc ccccgtgatc gcccggagca ggaactcggc
      661 ccgcgccccc cgggatcccc gctcgccggt ctcccggtcg acctcgaggt ccgcgtaccc
      721 tctcgccgag cgggccacgg cctccacggc ctcctcgggg agcggcgcgc gccccagccc
      781 ggtgtgcagc acgaccccgg tggcgttcac cactccagag aggcccagcg acgccgcggc
      841 ggcccggtgg acggcccggg ccatcagcgc ctcctcgtcc ggcatggccc gccggcggcc
      901 ggtcccgcgt ctcgtctcgt ccaggacctc gcggaccgcc gcccgcacca cgtcccgccc
      961 gaagcgctcg gatgcccgca cccccggctc ggagcggagg agggcatcca ccgacggcag
     1021 ccgccggcgc tgatcgacca tgcctcaacg atagcctggg agcggcccga gccccggccc
     1081 gcctcggtca gtcgctctcg gggatccagg gctccagctc gaccccgagg ccgtcggcga
     1141 tccgattcgc gtacgcgtag taggcgacca cctcgcagat gtggagcacg tcctcgtccg
     1201 agaagcccgc ctcccggagc gcgtctacgt cctcgcgact cacgtcggcg ggacgaaggg
     1261 tgagcttctc cgcgtactcg agcatcgcga tccgcttggg gtcgagcccg gcgccgcgcc
     1321 agtcgcgctc cacgcgagcc aacagctcgt cgtccttcag cagccggcgc agaccgcgcc
     1381 ggtggtgggt gattcagtag tggcactcgt tgacctggga gacgacgagg gcgatcagct
     1441 cgcgctccac cttgcggagg gtcttcgtgc tcttcatgac ctcccggtag agctcgtcgt
     1501 gggtccgcat cccgtccggg tggagggagt ggatctgcat gatcatgtcc acgcggccgt
     1561 acgtcgggtc caccatgcgc gcgtacagct cggcgagccg cccctcggcc tcgtcgtcgc
     1621 cccgcacctc gatccaggcc atgctcgaaa ccctagagga tgtcgtggag cgtgggctaa
     1681 gattcgcgcg ttgccgacaa gggagagacc gcccatggac accccggtca accgagcgag
     1741 ccgcgagaga ttcgtcacgc tgccgatcat ggaggagaag ggcttcgtgg agctcaggcg
     1801 ctacgagagc ccgcccgagc ccagcgagtg ggaggacctc gagtacgtcg actggaagtc
     1861 gggaggcgac acgaacttcg ccatcctggc ctcggccaac ggcgaccacg acccccgcgg
     1921 gttctgggag cacgggaagc ccgacaagga cggcgtctgg gtcgaggaca acatcgagaa
     1981 gtcccccacg atcaagaagt gggtcgagag cgtgggtgcg aacttcggcc gcgtgaggat
     2041 catcaagctc gagcccaaca cctacgagga cacggtcaag aacatgcacc ccgacgacaa
     2101 caaccggatc accgaggatg ggaccgggta cgtcgtgcgg gcctggctcc agctcaccga
     2161 caaccccgag tcctacatga tcgtccggga ggagaaggac gacgaggcct ccgagagccg
     2221 gatccccctg ccccgcggcg cgcagttcgt cgtggactcg gagcgcctgt ggcacgccgt
     2281 ctggcacaac gacgagcact acagcgag
//
LOCUS       DASUTZ010000217         3029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_723967, whole genome shotgun sequence.
ACCESSION   DASUTZ010000217 DASUTZ010000000
VERSION     DASUTZ010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723967"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VF097_09370"
     CDS             <1..906
                     /locus_tag="VF097_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEX6263042.1"
                     /translation="AKGMTLKAAAAGLDLGGGKAVIIGDPKRLKSEELLRAYGRFVDS
                     LGGRYITAEDVGTFREDLDIVRRETRWATGVSKLLGGSGDPSPVTAYGLFSGLKAAVQ
                     AGLGGKPFDQLHVTVQGVGKVGYGLVKHLVEAGSRVTVADVDVDAVARVVKEFGVETA
                     EPDKAHAVDCDVFAPCALGGVIRDDTLPELKCRVVAGAANNQLELPEHGDALHEAGIL
                     YAPDFVINAGGLINVADELIGYDRDRAMKKVEGIYRTLREVFRRSKTEGISPARAAEM
                     MAAERIEKVSRVRLLWVPRRNDGRR"
     gene            970..2049
                     /gene="pdhA"
                     /locus_tag="VF097_09375"
     CDS             970..2049
                     /gene="pdhA"
                     /locus_tag="VF097_09375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEX6263043.1"
                     /translation="MAQASDGAVRILAPNGSVEDHEARDALDLKDEDLRQLYRFMVVT
                     RKVDLESTALQRQGELAVYPPLIGQEAAQIGSGYALGDDDFVFPSYREMGVAVARGID
                     LVDYMHFHRGTWHGGIPDPVGKRFGMISVPVGSQPLHATGWAMGAKLDGKAACAITYF
                     GDGASSQGDVHEAMNFAGAFGAPVVFFCQNNGWAISVPIERQTAGEIYRRAEGYGFPG
                     VRVDGNDVLAVYRATREAAERARTESEPTLIEAITYRMGPHSTADDRSRYQPEEQIEA
                     WGTRDPIERFRRFLDAEEAADEGFYEEVEQEGKARAAEVRKGIAGAPPRPVEDLFKWV
                     YTGDELPPHLARQRDEALSEEGDDA"
     gene            2042..3016
                     /locus_tag="VF097_09380"
     CDS             2042..3016
                     /locus_tag="VF097_09380"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX6263044.1"
                     /translation="MPEVTMGQALTQALRYSLEHDDRVLILGEDVGKLGGVFRITDGL
                     QAEFGEKRVFDTPLAESAIAGVSLGLTMAGWKPVAEMQFDAFSYPALDQVISHMAKWR
                     YRSAGTLSVPVVIRIPEGGGIGAAEHHSESPETYYVHTSGLKVVVPSTALDAFTLLVR
                     SIQDPDPVIFMEPKSRYWSKESGELSPNGLPIGTARTMREGTHATLIAWGAMVQRCME
                     AAEAAEEDGVSVEVLDLRTLVPPDLETMAASVRKTGRAIVVHEAPMILGFGAEVVARL
                     MEDAFDHLEAPVLRVTGWDTPYPPATLEEHYLPNVDRILGALERVLTY"
BASE COUNT          471 a         1013 c         1128 g          417 t
ORIGIN      
        1 gccaagggga tgaccctcaa ggccgcggcc gccggcctcg acctcggcgg tggaaaggcc
       61 gtgatcatcg gggaccccaa gcgcctcaag tccgaggagc ttctccgcgc ctacggccgg
      121 ttcgtggact ccctgggcgg ccgctacatc acggccgagg acgtggggac cttccgcgag
      181 gacctcgaca tcgtccgccg cgagacccgc tgggcgaccg gggtctcgaa gctgctgggg
      241 gggtccgggg atccctcgcc ggtgaccgct tacggcctct tcagcggcct gaaggccgcg
      301 gtgcaggcgg gactgggcgg caagccgttc gaccagctcc acgtcacggt gcagggcgtg
      361 ggaaaggtgg ggtacgggct cgtgaagcac ctggtggagg cgggctcccg cgtgaccgtg
      421 gccgacgtgg acgtggacgc ggtggcccgg gttgtcaagg agttcggggt cgagaccgcc
      481 gagcccgaca aggctcacgc cgtcgactgc gacgtcttcg cgccgtgcgc cctgggcggc
      541 gtgattcgag acgacacgct gcccgagctg aagtgtcgag ttgtggccgg ggccgccaac
      601 aaccagctcg agctgccgga gcacggcgat gccctccacg aggcggggat cctctacgcg
      661 cccgacttcg tgatcaacgc gggcggcctg atcaacgtgg ccgacgagct gatcggctac
      721 gaccgcgacc gcgccatgaa gaaggtggag gggatctacc ggacgctccg cgaggtgttc
      781 cgccgctcga agaccgaggg gatctcaccc gctcgagctg ccgagatgat ggccgccgag
      841 cggatcgaga aggtcagccg ggttcggctc ctctgggttc cgcgccgcaa cgacggccgg
      901 cgctgacccg taggcgcgcc cgggccgcgt gccctatcct gagcccaccc gggaccagga
      961 ggcaccagct tggcacaggc atcggacggc gccgtccgca tcctcgctcc caacgggagc
     1021 gtcgaggacc acgaggctcg agacgcgctc gacctgaagg acgaggacct ccgccagctc
     1081 tatcgcttca tggtggtcac gcggaaggtg gacctcgagt ccaccgctct ccagcgccag
     1141 ggcgagctgg ccgtgtaccc gccgctgatc ggacaggagg ccgcccaaat cgggagcggg
     1201 tacgcgctcg gcgacgacga cttcgttttc ccgtcctatc gcgagatggg cgtggcggtg
     1261 gcacgaggca tcgatctggt ggactacatg cacttccacc gggggacgtg gcacgggggc
     1321 atccccgacc cggtgggcaa gcgcttcggg atgatctcgg tcccggtggg gagccagccg
     1381 ctccatgcca cgggctgggc gatgggagcg aagctcgacg gcaaggccgc gtgcgcgatc
     1441 acctacttcg gggacggggc ctcctcgcag ggcgacgtcc acgaggcgat gaatttcgca
     1501 ggcgcgttcg gcgccccggt cgtcttcttc tgccagaaca acgggtgggc catctcggtc
     1561 cccatcgagc gtcagaccgc gggcgagatc taccggcggg ccgagggcta cggcttcccc
     1621 ggggtccgcg tggacggcaa cgacgtcctc gcggtgtacc gggcgacgcg cgaggcggcc
     1681 gagcgcgccc ggaccgagtc cgagcccacg ctgatcgagg cgatcaccta ccggatgggc
     1741 ccgcactcca ccgccgacga ccgatcgcgc taccagcccg aggagcagat cgaggcgtgg
     1801 ggcacccgag acccgatcga gcggttccgg cggttcctcg acgccgagga ggccgccgac
     1861 gagggcttct acgaggaggt cgagcaggag gggaaggcca gggcggccga ggtccggaag
     1921 ggcatcgccg gagccccgcc ccggccggtg gaggacctct tcaagtgggt ctacacgggg
     1981 gacgagctcc ccccgcacct ggcccgccag cgcgacgagg ccctctccga ggagggcgac
     2041 gatgcctgag gtcacgatgg gccaggcgct gacgcaggcc ctccggtact cgctggagca
     2101 cgacgaccgg gtcctgatcc tgggcgagga cgttgggaag ctgggcggcg tgttccgcat
     2161 caccgacggc ctccaggccg agttcgggga gaagcgggtc ttcgacaccc cgctcgccga
     2221 gtcggcgatc gcgggggtgt cgcttgggct gacgatggcg gggtggaagc cggtggccga
     2281 gatgcagttc gacgcgttct cgtaccccgc gctggaccag gtgatctcgc acatggcgaa
     2341 gtggcggtac cggtcggccg gaaccctcag cgtcccggtc gtgatccgga tccccgaggg
     2401 cgggggcatc ggcgcggccg agcaccactc cgagagcccg gagacctact acgtccacac
     2461 ctcgggcctg aaggtggtcg tgccgagcac cgcgctggac gcgttcacgc tgctcgtgcg
     2521 ctcgatccag gatcccgacc cggtgatctt catggagccc aagagccggt actggtcgaa
     2581 ggagtcgggc gagctgagcc caaacggcct cccgatcggc acagccagga cgatgcgcga
     2641 gggcacccac gccacgttga tcgcctgggg cgcgatggtc cagcggtgca tggaggcggc
     2701 ggaggcggcc gaggaggatg gcgtctcggt cgaggttctc gacctacgca cgctggtgcc
     2761 gccggacctg gagacgatgg cggcgtcggt gcggaagacg ggccgggcca tcgtcgtcca
     2821 cgaggccccc atgatcctgg ggttcggggc ggaggtcgtg gcccggctga tggaggacgc
     2881 cttcgaccac ctcgaggccc cggtcctgcg ggtcacgggg tgggacacgc cgtacccgcc
     2941 ggccaccctc gaggagcact acctgccgaa cgtcgaccgg atcctgggcg cgctggagcg
     3001 cgtcctgacg tactgaggcc atggccgag
//
LOCUS       DASUTZ010000218        29688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1630630, whole genome shotgun sequence.
ACCESSION   DASUTZ010000218 DASUTZ010000000
VERSION     DASUTZ010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 29688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29688
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1630630"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VF097_09385"
     CDS             <1..772
                     /locus_tag="VF097_09385"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX6263045.1"
                     /translation="RQMFFRTRALEDAARALKDREVILRTLSDRMAEERQDERTQIAG
                     YLHDDLAQMLYRMSLHIDISRKHLTSGQPGEALEELGAIQETKDRTLQLIRSLIKDLH
                     RSPLGRAGLTEAISSFCEDLEKSSGIEFVLSLREAEMPPPIQLLCYQVTREAIMNIIK
                     HADASRVDVILEPTSEGARVTIADNGKGFDPEQGSPEGHFGLAMMRERAQISGGSLTI
                     ESTPGEGSTVVAEFSTAWLSDSDQESHRALGLSSSDPA"
     gene            complement(696..1295)
                     /locus_tag="VF097_09390"
     CDS             complement(696..1295)
                     /locus_tag="VF097_09390"
                     /inference="COORDINATES: protein motif:HMM:NF036410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 family protein"
                     /protein_id="HEX6263046.1"
                     /translation="MLVIGAFLAIGLLLGWGLNGGLRYLDDVRVRGWWLAPLALALQV
                     APAPTIEADWGRYVPAAMLALSFVVLLLVVAVNLRNRGFGLILFGLMLNLAVILVSRG
                     MPVSAYALEEIGAEDDIERLARAQPGAKHHLATDDDLLRFIGDAIPVRSPFDAVLSPG
                     DVLMYTGGAWFLVAAMRGRSRRDPELDDSPDPNRTATRY"
     gene            1607..2857
                     /locus_tag="VF097_09395"
     CDS             1607..2857
                     /locus_tag="VF097_09395"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6263047.1"
                     /translation="MNRVPMRSVAVVLLALALVAGACVEERNAPQGESGGESTEPIRL
                     GLVAPFSGTAASIGRNMREGMSIAIDELNAGGGVLGRQIELLEMDNEFDPAKTSEVTR
                     ELIQRQGVVALFGPPGTTSYLAIDDLVRQEEVPTFPVVTGPQLKEEVNPYTFRLMIPD
                     DIQIGLLAEYAAERFERVAILAEDNETGRSIAEIADEELSSRGKSPVSVEFFSEDELD
                     LSPIVLEASQADADAVIIGSHIGPYAARIATAADSLGYDAQLLGLAGLTSYTYADLAR
                     DGAVGTVFVAPPIPVLAGTDTPAAQAFHEAYVGRYFPDGTKSDSGADKVTGAAYLTYD
                     GVMMWARAAEDAGSADPAAVVEVFNAGFDFGPEESSAGIAWHYDANDHEGFHEGDTWF
                     YQWDETPAGIEFTFLGDAAEILAG"
     gene            2872..3435
                     /locus_tag="VF097_09400"
     CDS             2872..3435
                     /locus_tag="VF097_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263048.1"
                     /translation="MRRAIVAGGLLGVLTGLLFVYHEALRLSVAWPVVAGLALWGSVG
                     RDGSRGLNTAVAAAVGVGAGYASYAVVAEFLPVTRMWLGITIGVLVGVLVLVGVWSRW
                     FPLAGALIGFAVFAGLFEPLWAESPAAVRTRGIETLTVALLGVLTGIVAATLVRAVAE
                     RAAPETEAERDEEEAPAPAGDVVGGPA"
     gene            3432..5891
                     /locus_tag="VF097_09405"
     CDS             3432..5891
                     /locus_tag="VF097_09405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263049.1"
                     /translation="MRRLGTWMTAFLLFGLFAVAGAPSAVIAQTDTANKVVDYETIFA
                     SLDADGSLRSVRLVDQLRVFGEGDILVTDPAPTEDFRNLSGTGDPEVGEGTVDWPIQD
                     LSGSAEFLTASTPAVDPPLSMVVAYYLNGEPIDGPDLVGSGGTIDMAFDVSNTTSQSM
                     EVTYEDGAGNTRTAIEQVPIPMVAELQMELEKERFSVVEAPGADIITDPNGNLVVRWS
                     MVMVPPIGELTQSFTVRLDTEDFQLGPVRLTGVPVAPRDRPFLAFAEEELESGHESAA
                     SLFAGSGELSSNLEALHDGTLDLLDGMQQLFDGSKELATGLQEAASGSGRLAEGAGEA
                     TVGSGQLTAGLGDALSGSSQITGGLTDLSKGLKRLFRGAQRLADGLPAAQAGATEIAL
                     GAQALDVALAALKAGLEMAETGGVQISAGAAAIRDCLVGGGTPCSGNPSVNAIAGQIA
                     GGAGQIETGAGQIQTGAGQIEAGAGQIQAGAGGIQGAAGTLEGACIGDTGAASCPTVA
                     AIAAAISAGAGDIGTGAGAIVVGAQGIGVGAGQIVGGAQSIQTGAVGIQSIVAGLAAA
                     LDQIIAGAQQIEGGLGQAIAGIGLPSDAPDASIRGGLNAIRQGALQLADGMADAIAGI
                     GDEDTPDTLLFGANAASEGADLLRAGSAQLTSGLGEAHSGSGELTAGLTQISDGAGQL
                     ASGLGEASSGSSRLSKGIGDAKDGAQQIEQGVYLVNEVGVKEVSASANETAAELDRTL
                     QFMHAQDERAANESLLYGPPSSDQAEVVVGGSGVVLTMDALDARSAESTARGIGALIA
                     LVILGGLLLVIRGVRRTPSPA"
     gene            5950..6813
                     /locus_tag="VF097_09410"
     CDS             5950..6813
                     /locus_tag="VF097_09410"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6263050.1"
                     /translation="MTLFLQQLFAGLAQGSVYALVAFGYALIFSTSRVVNFAQGHLFM
                     LGAMTGVALLGIGVPYLLMIPLTMAIVAASGLALERSITLPLRATRSAYTWIVATLAA
                     AVVIENVASMIFGKEPRTFPPAIAGVTRIGDLVIQHHAVVVLGVALAVMIGIELWLGR
                     TMIGTAFRATADNPAPARLSGVPVPRLVMLSFALSGAIAGLGGVLVAPITFAAVPIGL
                     LLGLKGFIAAVIGGMGSFRGALVGGLSLGLAETVIRQQLPAGMGGTILFGFLLLTLVM
                     RPTGLFGRRLA"
     gene            6810..8600
                     /locus_tag="VF097_09415"
     CDS             6810..8600
                     /locus_tag="VF097_09415"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEX6263051.1"
                     /translation="MRARRASFWGGVALVFLGASLVYLPVGEFYLFLASRSACFLLVV
                     AGLNLALGYTGLFSFAHVALFALGAYATAIAVTRQGLPVWTGIGAALLVGAVAGAVLT
                     LATFRARAIHFAVVSLVLVFAVSELLSGWTSVTGGEAGITVRRPELAGDALLARRYWL
                     FVLAVAAVCLLAIRNLVRSPLGRALVAVRESEEAAASVGIDPFRYRTVAMAFGGAVAA
                     AGGALFAHLDGFVSPEISGFDDATLFVAMLLVGGAGALWGPVLGVGAFLVIDRVLVAL
                     RGALPGLDLVVWQALISGILLFLVIVFLPRGMAGALTSRRREATVADRDEPPAAAVLP
                     APRIPWPEGPALEVVGLTKRFGGVRAVDDLDLVVEGGTIHGLIGPNGSGKTTTVDLLT
                     GALAADAGEIRFLGERVVRPRPHAMAARGLGRVFQRTEVFAAVASLENVMAGFHLVAE
                     RGLPANVLRTPGARRRDEELRREAATLLEALGLAERRHLLAASLPFGDRRLLEVARAL
                     AARPTVLILDEPATGLSTGELGRLAALLARLREGGVTVLLIEHNMEFLMGLCDRVTVL
                     DSGRRIAEGTPTEVQSDPKVVEAYLGEEAG"
     gene            8597..9325
                     /locus_tag="VF097_09420"
     CDS             8597..9325
                     /locus_tag="VF097_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011434810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6263052.1"
                     /translation="MTASPLLRIQGLTCSYGAITALRGVDLDAHPNEVTCVLGVNGAG
                     KSTLLAALAGAIRPSAGSVRVGEVETVGTSPERMVGLGVVLVPEGRQVFPSLTVRDNL
                     LIGAYRLRRDRSAVLEGLDEVRRLFPILGELAERPAGALSGGEQQMLAIGRALMGRPR
                     VLMLDEPSLGLAPRVVRVVMEAVAGLAAEGRAVLLVEQLARVALQSSHRGYLLESGTV
                     VLEGTSEDLQADDRVREIYMGVKG"
     gene            9322..10959
                     /locus_tag="VF097_09425"
     CDS             9322..10959
                     /locus_tag="VF097_09425"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6263053.1"
                     /translation="MRTTEPLAPEREATDRSRTGSWIRLIAAVLGLAVIGVAARMLAD
                     FQWNRVVEYRSPYVFDLPAAPETPRLTDRVILVVVDGLRVDASERMPTLQRLGGEGSS
                     LVARTGLPSLSFPGWTNLASGAPPDVSSVTTNWYEGRVRVDSLLASARRANLTTAVAG
                     GEGWRQLFRGQIDHSFYVPDARRFADERIGRAALRILAEDDPDLLIVHLPDVDHRGHE
                     TGVNGPYHDAALGADGVIGALVEATEDNAAIIVTSDHGHIESGGHGGPEEEVVRTPLV
                     LWGDGLVPGASGAVSHPDVAPTIAALLGVGRPAHATGQLRVELLDADDQTRAQIESAH
                     DETLARFYQAATGALEASGSTEAAFERARQDRHRHDVLARLPIALGVAALLAIAVALA
                     TRGLDAVAILVGSIGFFGTWAGIFLARGLSFSFSHFNTEEQVEPFLVARLLDTLIALG
                     VASVLAGLVAGRRQRAGGFQTGLGVAAWILLITGMAVAGFLVVFGWGFVWGLPNLVAG
                     FAEFLALLAMFGVGVAAWAAGLVSLGTAAVVAPRRAR"
     gene            10993..11469
                     /locus_tag="VF097_09430"
     CDS             10993..11469
                     /locus_tag="VF097_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061136 - regulation of proteasomal protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome activator"
                     /protein_id="HEX6263054.1"
                     /translation="MDEEQRTPDVIVPGMTKDEDDSQEIVEHPAKLLRIASMIRELLE
                     EARHSSLDEGGRKRLQEIYDRALVELKDVLTEDLRRELDALAIPFTDTPSESEIRVAQ
                     AQLVGWLEGLFHGIQAALWAQQMQARQQLEEMRRRGLPSGLPGQPQQGQSPPGQYL"
     gene            11471..11839
                     /locus_tag="VF097_09435"
     CDS             11471..11839
                     /locus_tag="VF097_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263055.1"
                     /translation="MDELHAFVGFAIVGGFALLSLWGLITWLIRRGPGKAFWWVVGTL
                     QGALIVQLLVGVVLLALGGGRGILHYLYGVGFPALILVGAHWLAREAFADRPWAPFAV
                     GSFFIFGLTLRALMTGLGIG"
     gene            11952..12185
                     /gene="infA"
                     /locus_tag="VF097_09440"
     CDS             11952..12185
                     /gene="infA"
                     /locus_tag="VF097_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX6263056.1"
                     /translation="MERAGPQKEDALQVEGTIIEALPNVMFRIDLDNGHNILGHISGK
                     MRKHYIRILPGDRVTVELSPYDLTRGRIVYRHR"
     gene            12205..13344
                     /locus_tag="VF097_09445"
     CDS             12205..13344
                     /locus_tag="VF097_09445"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX6263057.1"
                     /translation="MTPRRTLALGATVALVLLLWWASGLDRSSAPADRFFSQPEIARA
                     SEYRTPLYAAAALRLSLDLGLLAALAFTGAGAWLGRLRGSRWIRGAALAIVAIVLLRF
                     VVRLPISFWAGYVHEHSWGFSTQTVWGWLGDAGRSVGLALAVSIPVYVAALILLGSFV
                     WPVVVEPLFNRFEPLRGRAADRFEDLADRAGVPVDAVLVADASRRTRKENAYVSGFGG
                     TKRLVLYDTLLRSASQEEAEVVVAHELGHRRGGHVLRGTLVAALAGAGAILLIALAAR
                     TRWLRRISGETGPDDPRMLPALLLGISLLSLAALPPSNLLSRSFEEEADRAALALTGD
                     RRAYEAAHRGLALRNLSDLDPGPVVYRFLATHPTAPERIAYALEP"
     gene            complement(13326..14342)
                     /locus_tag="VF097_09450"
     CDS             complement(13326..14342)
                     /locus_tag="VF097_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEX6263058.1"
                     /translation="MHVVATGTHRDHAPRTEFVLGEVVPAYEVPERVDRILEAVSEAH
                     PVSEPTEHGRSPLERIHDPAYLDHLASAWRDWVAEGGAGPLAADTFALPDVVAGPRPR
                     GAEARVGAYTFDASTPILEGTWRAAAAAADCALTGADLLLGGERAAYALCRPPGHHAG
                     RTFAGGYCYLNSAAAAAAALREEAGPVAILDVDYHHGNGTQEIFRDDPTVLYVSIHAD
                     PDVEYPYFTGREADGGEATVNLPLPLGTGDDVYLQAAERAFARTADHAPEALVVSLGL
                     DAHEDDPMGRFALTDEAFRALGSMAAALGPPVLIVQEGGYLSESLGGAVRAFLDAVDQ
                     GSSA"
     gene            14578..15210
                     /locus_tag="VF097_09455"
     CDS             14578..15210
                     /locus_tag="VF097_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263059.1"
                     /translation="MNEQSLRESNGILAAAADRAAVGELIPATPAELAREVGVDDRLA
                     VARAVRALLARGRLEQEGDRYRLLDTRPLERGEPATVRRPSRRRARSREAETQDEGFP
                     TYEQVGKAIVERLIELSAESAELRASLERARSESEAARREALEATRAASRERQRATDL
                     EQESRTLRQRLEMTESNLRRIIEAAQSRPAARLEDSDAKAILDVLSKKDA"
     gene            15332..16627
                     /locus_tag="VF097_09460"
     CDS             15332..16627
                     /locus_tag="VF097_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263060.1"
                     /translation="MPGPIVGWVSLIAQIPSDFLGLSASQWFWVAIVGWSLGVVLGGL
                     LAARAVQGAPGQGEVSPERIALDTPAILRAVAPDEDAAGGVLRWLLGEVDADAVAHLE
                     LSPLGETLRIEPRGLEGASVARLAELARSALLGSEPLAEGVGVARWVGAGGTNALVAT
                     GTAIPGHEEPLRFSRYLLEWVRSAERGRPRGDLEDRLREVSGVTWVEVVGRSARLLAE
                     DEASAEVALAEVARRLEGTGVAPVWIDAAEPRPDVPTGGPVAGAVGGPEPPVVAIPEA
                     ERSEAQRIPTEPIGMKESLVASAIGAQEPRIRLLDVALRENGEATADVRVSWNEQEIR
                     GRGHGRATRAGRSFAAAQAVADALRPLLDTDVVIEGLYVATTKEGLDVLISEVKMEGQ
                     RYVGAVLERHDRPDWTGARAVLDAVNRRLVQVAGRSGRI"
     gene            complement(16632..17981)
                     /locus_tag="VF097_09465"
     CDS             complement(16632..17981)
                     /locus_tag="VF097_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263061.1"
                     /translation="MEGRSSEARWIAYVRAGEQVGPGSLIAVRPGGGGERTLLDAGVV
                     GADAGGAGAVFALVRRGETTELLRVRIGSGAADGPRATGSLSGIAAARGRVAVERRVA
                     SGSDVPRHLRGALDELATTSITVLAPPRRPPGTTHTVASAGGRRYELRFTNDPDGVLS
                     HAEQVNVFVGASPRPVAPLPDALPVEVRGTDGSFSCGASACFLDWEEGSSYAVGEFGR
                     PEDAVEFAESLRPMEELIGPSWREGGAVAAPQLVVLGGPEETVLESVEGYCECSFHPM
                     DWDDAGERLLVVTRAEGFTTVTEYGRPDGPEVLHEAAGRAILDVSYTPEGVAIIESPA
                     VGRPGPLRTLDGRTIARRAISFDVEGSTMAVVRGNGDVLVRDLETGAEQVVGTGAISV
                     TVSPGGVAPAPSEGAPQRPPQPSDFGDPTVPFGVAVAALAGASVLGGATLLLIRRRR"
     gene            18068..18763
                     /locus_tag="VF097_09470"
     CDS             18068..18763
                     /locus_tag="VF097_09470"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6263062.1"
                     /translation="MSPKDPDIRPDRPGSIRAMFTDLAPDYDRLNTLLTFGRVRSWRR
                     TLVRAAGVRFGDRVLDVCAGTGASLAAVREAVGARGVAVGVDFTPAMLLRAEGVRVLG
                     DALRLPFLDRSFDATVSAFALRDVADQPACIAEMFRVTRPGGGVAILEVGRPHRQPMR
                     AGFDLWFRGAAPRLGAALGQGESHRFLARSIGYLPEPEALLGMLRDAGLVGVRARDLT
                     LGAARLFSGFRPA"
     gene            complement(18750..18908)
                     /locus_tag="VF097_09475"
     CDS             complement(18750..18908)
                     /locus_tag="VF097_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263063.1"
                     /translation="MSETRLPSGDPLSTRTRPEEGVWELRVIEDDLEFTLWSDEPVLI
                     EDSDLTRA"
     gene            complement(19135..19695)
                     /locus_tag="VF097_09480"
     CDS             complement(19135..19695)
                     /locus_tag="VF097_09480"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HEX6263064.1"
                     /translation="MRRVVILALALAACGEPAVTVVPEAELPQDVFGSPRPETLTELP
                     ESTIVYFVLGGRLIAVPRQLPAEAPSLPAAAMEALLQGPSSPWKTAIPEGTRLLSVEV
                     DGIVATVDLSDEFELSAPGHRLALRVAQVVYTLTETPPVEAVLFRIEGSPADVLTGRD
                     EVVQGSVTRAHYQRFAPLERDESEAD"
     gene            complement(19692..21083)
                     /locus_tag="VF097_09485"
     CDS             complement(19692..21083)
                     /locus_tag="VF097_09485"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6263065.1"
                     /translation="MARRRRPRGLRRRIMVAFIGGALGVSALVAGSTYYLADRYVVNQ
                     RIEAATEQSFSNIAFAVRYVEPEQASLAELAGLLEARGSFDVLLMKGEQWFPSTLSIT
                     QRSVPAPVLEEVRRGQVAYRFQRDEPFLVFGSPIPGSDITAFFFYPVRDVAEALALLW
                     KVLLMVSLGAVMLAAIAAVRVSRRVVHPVRLASDAARRVAEGLLATRLPVAERDEIGT
                     MAHSFNEMAAALEERIARERRFVGDVSHELRTPLTTLRASADYLLERADELPEQHERA
                     VELLSADLDYLRRLVDDLLDISRLESGPIQLSLEPLNVGDLVREVVARRSRAADGEVR
                     VEVNGGGETLTTLADKRRLERVVGNLVDNALTHGDLPVTVRVGAANGTITLAVEDRGP
                     GIAGQDLPRIFDRFYKADPSRPRLDGRGSGLGLAIAQENAKLHGGEIEVESSPGAGAR
                     FLFRLPRVSRTGA"
     gene            complement(21083..21772)
                     /locus_tag="VF097_09490"
     CDS             complement(21083..21772)
                     /locus_tag="VF097_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6263066.1"
                     /translation="MTGQLILLVDDDARLREIVGMALEGEGYRVLPAGSAEEASDLLA
                     REEPDLLILDVMLPGQDGFELCRDVRTRSPVPILMLTAKTDTVDVVVGLESGADDYVT
                     KPFVTKELVARIRALLRRSRGPEDGNGQRRIAVGDLEILPDAASVTKAGEEIHLTKTE
                     FRLLTTMASRPNQVFTREMLLSQVWEYDYFGDSRIVDVHIRRLRSKIEADPRRPEIVQ
                     TVRGIGYKVVG"
     gene            complement(21815..22843)
                     /locus_tag="VF097_09495"
     CDS             complement(21815..22843)
                     /locus_tag="VF097_09495"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX6263067.1"
                     /translation="MLGPLVVLLAVGAGWVLRTQLIEALPTAPETAIASPAPSPSDAP
                     TSAPTPELAPTPPSRGRLMIHGTGDVSLDPSYIPNLSTQGYGYAWSGLEDLFREDDLT
                     VVNLECPVSDLGTPVPKEFSFRCDPAALPAARRAGVDVANLANNHGGDYGPEALLDSR
                     RRLREARIAPVGVGRNVAQATRPVIIETKGWRIAVLGFGGVIPSPAWIAAENAPGMAD
                     GDSIENMTRAVRAADRRADLVIVTVHWGTELSTAPSPDDVERAHAMIDAGADAIFGHH
                     AHRLQPLEHYKGRPIAYGLGNFVWPSFSHEGATTAVARVVVTPSGKMRGRLVPAFIED
                     HGHPVLTG"
     gene            complement(23042..23512)
                     /locus_tag="VF097_09500"
     CDS             complement(23042..23512)
                     /locus_tag="VF097_09500"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX6263068.1"
                     /translation="MSNPMDPIREEHRHLLPHIQSLRRAADLAPGDDARKAAEDAHRF
                     LVEHLLPHAKAEDRVLYPAVEEAMGAPGAAATMSRDHVEVETLTGDLGDLLPRLEDDP
                     GDAVWMELRRILYGLHALVAVHFAKEEEIYLPILDARLSAEEGERIVHEMHGEH"
     gene            complement(23542..24009)
                     /locus_tag="VF097_09505"
     CDS             complement(23542..24009)
                     /locus_tag="VF097_09505"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX6263069.1"
                     /translation="MATPPEEGFRPLPSHYDHCFGCGREHLTGLHLRMEGGGREVRGS
                     FTVTEHHQGAPMLAHGGVVAAAMDEGMGYLLYLLRTPAVTGHLEVDYRRPVPVGATLE
                     LRGTLEKVEGRRIHARMTGYVGEDLAVEARAVYVEVSLDHFRPDRGLEAPYNP"
     gene            24133..24513
                     /locus_tag="VF097_09510"
     CDS             24133..24513
                     /locus_tag="VF097_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263070.1"
                     /translation="MRKGRLWKLVAGGSAIGIGAAAGIAVAGTDAQAPQPVELGETGE
                     TSTLDVEGTPVTVASPSPLGDSLDDGNAGASVDTSGSASSADTSGSADTSGSTDTSGS
                     ADTSGTDTMDGADSPDDDNSGPGS"
     gene            24513..25757
                     /locus_tag="VF097_09515"
     CDS             24513..25757
                     /locus_tag="VF097_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX6263071.1"
                     /translation="MPERGIPRDEALPGAEGLTGPLGARAVASFLEARGWRATASRPV
                     QALYRPGREAVARHRVQAEGPGGGRRVLTIVTEVRARRRDPVPPPSWFEDRFGVPEPV
                     GEVGRFLVWAFPFDPGLPALPEAADGRSVRDRLRHAAPAAVAVQPLTYRPRRRAVFRY
                     RVVGSASEVLYGKVLRQRKLRRAREVMRRVRGRRVRLGTATEAEGLLLSQAVPGRSLR
                     DVLVRGGPVPSPARVAGLLGSLEAPGVGGEAPDPVADAGTTASLIERIVPQAAGPAAR
                     VADRVERGAAGTPSPVGTVHGDLYEHQVLVDEGFSLGLVDLDDVGPGDLAVDAANFTA
                     HLVALAMAVPAAARRLLAYRSILVPAFAARLDVDVRDLAWREALSMLKLSVGPFRVLQ
                     PAWQAEVQRRVEVALRLSQGWS"
     gene            25748..26431
                     /locus_tag="VF097_09520"
     CDS             25748..26431
                     /locus_tag="VF097_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6263072.1"
                     /translation="MVVSRTHVLLIEDDARIRDIVEQGLGARGFVVTSAPDAGTGVDL
                     AGKLDVALILLDLVLPGRQGLDALGTLKELKPDVPVIALTALDDTGSKVGGLDAGVDD
                     YVTKPFSVEELAARIRARLRDRDDGEGVMKAGALTVDLAAHRAVLQGREVALSARELS
                     LLAAFMRHRGQVLSRDQLLRLVWDMDFDPGSNVVEVFVAALRRKLGGGIIETVRGTGY
                     RFVGPQEEA"
     gene            26428..26739
                     /locus_tag="VF097_09525"
     CDS             26428..26739
                     /locus_tag="VF097_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263073.1"
                     /translation="MRVAVAAFLGALLFLAGAVYGSGAVVPESQPPQAVVLVDDASPA
                     PAASPEPTPTPTNDDDEVEEVEHEVEEDDDHSGRGRGRGGDDDEEEEDDDDSSGPGDG
                     G"
     gene            26729..28132
                     /locus_tag="VF097_09530"
     CDS             26729..28132
                     /locus_tag="VF097_09530"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6263074.1"
                     /translation="MAARRSLPLGPRLVLTHVLIAGAVLLVVAALVFHLTRVHLLRGL
                     DARLSIAVQSFQQGPARQVARPADLPNAARSWLAVHGFTSKEVAAVRTSAGPVLSAAG
                     GMPVSDLPPEVLDARSSRWWEIEMSGEPVRALTVPLLLGDRTVGTLVVGSSRADIQAT
                     LDALVRGMLIAAGLGLALAAGLGLAAVRRTLRPLRRMTMRTDEIEATGDLSLRVSGDG
                     PRDEVGRLAESFDAMLERLDEAFRSRQRFVADASHELRTPLTVVRGQLELLRDRLEAE
                     GDRASVGLAMDEIDRMRRIVEELLLLARLDEGLPLERSPVEVELLMQEALVRGLAGER
                     RRASVDAESGLLALADPERLLQVLTNLVANAVKHAGEDARISLRARRANDRVVVEVAD
                     TGRGIPPDELPHVFSRLFRGTRARGEAGAGLGLSIASSLLEAMDGRVRVSSREGAGTV
                     FTVELPLAAPATAPAEA"
     gene            28156..28668
                     /gene="greA"
                     /locus_tag="VF097_09535"
     CDS             28156..28668
                     /gene="greA"
                     /locus_tag="VF097_09535"
                     /inference="COORDINATES: protein motif:HMM:NF015414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX6263075.1"
                     /translation="MTTRPPRRPGEEPTAVLTRDAYERLRGELDRLRGEGRREMAERL
                     ERAREHGDIRENAEYDAAKDAQGLMEARIREIERLLRDPDIVEGAASTDAAGAGVLIT
                     VRPLDDEDDQEERFLLAASKEEKAEGARTVSLSSPLGEALAGKKVGEKVTYEAPGGTF
                     AYEVLSLEIP"
     gene            28665..29087
                     /gene="arfB"
                     /locus_tag="VF097_09540"
     CDS             28665..29087
                     /gene="arfB"
                     /locus_tag="VF097_09540"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014911236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEX6263076.1"
                     /translation="MNVRVNRRVTIPASELSFRFTPSGGPGGQHANRSATRVELIWNV
                     EESAALGPRQKARVRRALRGRIDAEGNLRLVSGAERSQARNRADVVERLGRLLEGALA
                     VPKRRVGTKPSAAARERRMKAKRRRSEVKRLRRTPPVE"
     gene            29123..29452
                     /locus_tag="VF097_09545"
     CDS             29123..29452
                     /locus_tag="VF097_09545"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX6263077.1"
                     /translation="MSLLIGGTLALLFLPAPWSIVVIVGLAGFEVLEVAFWLWLRRQR
                     PRSGHEALVGRHGVLVEGGRVRIAGTTYPGRVLEGVPGDPVIVEEVDGMILVVRRGEP
                     RYEEEIA"
     gene            29449..>29688
                     /locus_tag="VF097_09550"
     CDS             29449..>29688
                     /locus_tag="VF097_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263078.1"
                     /translation="MSGHPLEPTGAELRAMVEEAVSYLASFLDELPGAPAAGAPDKEA
                     LAETLREDHPPAAGAPLADALSVIREAVPRAFDTTG"
BASE COUNT         3916 a        10276 c        11165 g         4331 t
ORIGIN      
        1 tcgccagatg tttttcagga cgagggcgtt ggaggatgcg gcgagggcgc tgaaggatcg
       61 cgaggttatc cttcgcaccc tctcggatcg gatggcggag gagcggcaag atgagaggac
      121 gcagatcgct ggctacctcc acgacgacct cgcccagatg ctctaccgga tgtccctcca
      181 catcgacatc tcgaggaagc acctaacctc tgggcaaccg ggcgaggccc tggaggagct
      241 cggagcgatc caggagacga aggaccggac cctccagctc atccgctcgc tgatcaagga
      301 cctgcaccgg tcgccgctcg gtcgagcggg cctcaccgag gccatcagct cgttctgcga
      361 ggacttggag aagagctcgg ggatcgagtt cgtcctgagc ctgcgcgagg cggagatgcc
      421 cccaccgatc cagctcctct gctatcaggt cacacgcgag gcgatcatga acatcatcaa
      481 acacgccgac gcctcccgcg tggacgtcat cctcgagcca acgtccgagg gggcccgggt
      541 cacgatcgcc gacaacggca agggattcga tcccgagcag gggtcacccg aggggcactt
      601 cggcctggcg atgatgcggg agcgcgccca gatctcggga gggtccctga ccatcgagag
      661 cacgccgggc gagggcagca ccgtcgtcgc cgagttcagt accgcgtggc tgtccgattc
      721 ggatcaggag agtcatcgag ctctgggtct ctcctcgagc gaccccgcat agccgccacg
      781 aggaaccagg cccctcctgt gtacatcaac acgtccccgg ggctcagcac tgcatcgaac
      841 ggcgaccgca ccgggatcgc atcgccgatg aaccggagga ggtcgtcgtc ggtcgcgagg
      901 tggtgcttgg ctcccggctg cgccctggca agcctctcga tgtcgtcctc cgctccgatc
      961 tcctccagcg cgtaggcaga gaccggcatg cccctgctca cgaggatcac agcgaggttc
     1021 agcatgaggc cgaagaggat cagcccgaat ccgcggttcc gcaggttgac cgcgacaacc
     1081 aagagcagaa cgacgaacga gagcgcgagc atcgccgcgg gcacgtagcg accccaatcg
     1141 gcctcgatcg tcggggcagg ggcgacctgc agggcgagcg ccaacggggc gagccaccat
     1201 ccccgcaccc gtacgtcatc caggtaacgc agccctccgt tcagtcccca gcccagcagg
     1261 agacctatgg cgaggaaggc cccgatcacg agcatgcagg ctccgtcccg tcggccggtg
     1321 tcgcgctggc cccaaggcca ggacccgtcc cccatgatag gggggaggac cccccgcgtc
     1381 ccccgatgtc ccttcggcta tcgggtttga ttcttccggg ccatttacct accatctggt
     1441 aactacgtat gctccgccgg gtaccgccgc gaggccctcg cctcgccgga tcgaagggac
     1501 gaacgcgtat gagaggggcc ggcgtcctgg cagtccgtcg acccttgctg cttgctcccc
     1561 ggccctcgcc ccagctcatg cgctcacagg atcgaggagg ttcacgatga atcgtgtccc
     1621 gatgcgttcg gtcgcggtcg tcctcttggc gctggcgctg gtggcgggcg cctgcgtgga
     1681 ggagcggaac gctccccagg gcgagagcgg tggcgagagc accgagccaa tcaggctcgg
     1741 gcttgtcgct ccgttctcgg ggactgccgc gtcgatcggc aggaacatgc gcgaggggat
     1801 gtcgatcgcg atcgacgagc tcaacgccgg cggcggtgtg ctcggccgac agatcgagct
     1861 cctggagatg gacaatgagt tcgaccctgc gaagacgagc gaggtgacgc gggagctgat
     1921 ccagcggcag ggcgtcgtcg cgctgttcgg tcctcccgga acgacctcgt acctggccat
     1981 cgacgacctg gttcgccagg aggaggtgcc gacgttcccg gtcgtgacgg gtccgcagct
     2041 caaggaagag gtgaacccgt acacgttccg tctgatgatt cccgacgaca tccagatcgg
     2101 cctcctcgcc gagtacgcgg ccgagcggtt cgagcgggtc gcgatcctgg ccgaggacaa
     2161 cgagaccggg cggtcgatcg cggaaatcgc ggacgaggag ctgagctcgc gaggcaagtc
     2221 ccccgtgtcg gtggagttct tctccgagga cgagctggat ctctcgccga tcgtgctgga
     2281 ggcgagccag gcggacgccg acgcggtcat catcgggtcg cacatcgggc cgtacgccgc
     2341 gaggatcgcc acagcggccg attcgctcgg gtacgacgcg cagctcctgg gcctcgccgg
     2401 gctgacgtcc tacacgtacg ccgacctggc tcgcgatggc gccgtgggca cggtgttcgt
     2461 tgcgccgccg atcccggtgc tggccgggac ggacacgccc gccgcccagg ctttccacga
     2521 ggcctacgtc gggaggtact tccccgacgg gacgaagagc gactcgggag cggacaaggt
     2581 gacgggggcc gcatacctga cctacgacgg cgtgatgatg tgggcccgag cggccgagga
     2641 cgcgggcagt gccgaccccg cggcggtggt cgaggtcttc aacgcgggct tcgatttcgg
     2701 gccggaggag tcgtcggccg gcatcgcctg gcattacgac gcgaacgacc atgaaggttt
     2761 ccacgaaggg gacacctggt tctaccagtg ggacgagacc cccgcgggca tcgagttcac
     2821 gttcctcggt gacgccgcgg agatcctcgc cggctgaagg agggatgaga gatgcgcaga
     2881 gcgatcgtgg cgggcgggct gctcggcgtg ctgaccgggc tcctgttcgt ctaccacgag
     2941 gcgctccggc tctcggtggc gtggccggtg gtggcgggcc tcgccctctg ggggtcggtg
     3001 ggccgtgacg ggtcgcgggg gctcaacacc gcggtggcgg cggcggtcgg cgtcggggcc
     3061 gggtacgcct cttacgccgt ggtggcggag ttccttcccg tgacgaggat gtggctgggg
     3121 atcacgatcg gcgtgctggt gggcgtgctg gtgctcgtcg gcgtttggag ccggtggttc
     3181 ccgctggccg gcgccttgat cgggttcgcc gtcttcgccg ggttgttcga gccgctgtgg
     3241 gcggagtcgc ccgccgccgt gaggacccgc gggatcgaga cgctgaccgt cgcgctcctc
     3301 ggggtgctga ccggcatcgt ggccgcgacg ctggtgcgcg cggtggcgga gcgggcggcc
     3361 ccagagacgg aggcggagcg agatgaagag gaagcgccgg ccccggccgg cgacgtcgtg
     3421 ggaggtccgg catgagacgc ctcgggacgt ggatgacagc gttcctgctc ttcgggttgt
     3481 tcgccgtggc aggtgcgccc tcggcggtga tcgcgcagac cgacaccgcg aacaaggtcg
     3541 tcgactacga gacgatcttc gcgtcgctgg acgccgacgg atcgcttcga tcggtgaggc
     3601 tggtggacca gctcagggtg ttcggtgagg gcgacatcct cgtgacggat ccggcgccga
     3661 cagaagactt ccgcaacctg tccgggacgg gcgacccaga ggtcggcgag ggcacggtgg
     3721 actggccgat ccaggatctg tccgggtccg cggagttcct gacggcctcg acgcccgccg
     3781 tcgatccacc gctctcgatg gtcgtcgcct actacctgaa cggtgagccg atcgacgggc
     3841 cagacctggt aggcagtggc gggacgatcg acatggcgtt cgacgtctcg aacacgactt
     3901 cccagtcgat ggaggtgacc tacgaggacg gggccggcaa cacgcggacg gcgatcgagc
     3961 aggtgccgat ccccatggtg gccgagctcc agatggagct ggagaaggag cggttctcgg
     4021 tggtcgaggc gcccggggcc gacatcatca ccgacccgaa cggcaacctc gtggtccggt
     4081 ggagcatggt gatggttccg cccatcggcg agctgacgca gagcttcacg gtccgactgg
     4141 acacggagga cttccagctg gggcccgtgc ggctcaccgg ggtgcccgtg gcgcctcgcg
     4201 accggccctt cctcgctttc gccgaggagg agctcgagag cgggcacgag agcgccgcct
     4261 ccctgttcgc ggggagcggc gagctgagct cgaacctgga ggccctccac gacgggacgc
     4321 tcgacctgct cgacgggatg cagcagctct tcgatggctc gaaggagctg gccaccgggc
     4381 tgcaggaggc cgcgagcgga tccgggcgcc tggcggaagg ggcgggcgag gcgaccgtag
     4441 gatcgggcca gctcacggcc gggctcggcg acgccctcag cgggtcgagc cagatcaccg
     4501 gcgggctgac cgatctgagc aaggggttga agcggctctt ccgcggggcc cagaggctcg
     4561 cggacggtct gcccgcggct caggcgggcg ccaccgagat cgctctgggg gcgcaggcgc
     4621 tcgacgtggc gctggccgcc ctgaaggccg ggctcgagat ggccgagacg ggaggggtcc
     4681 agatctcggc cggcgccgcc gcgatcaggg actgcctggt cggtggcggg acgccctgct
     4741 ccgggaaccc gtcggtgaac gcgatcgccg ggcagatcgc cggcggcgcc gggcagatcg
     4801 agaccggcgc ggggcagatc cagaccggcg ccgggcagat cgaagcgggc gccgggcaga
     4861 tccaggccgg tgccgggggg atccagggcg cggcggggac gctcgagggc gcgtgcatcg
     4921 gagacacggg cgccgcctcc tgtcccaccg tggcggcgat cgccgctgcc atctcggccg
     4981 gcgcgggcga catcgggacc ggcgcgggcg cgatcgtggt tggggcccag ggtatcggcg
     5041 taggcgccgg acagatcgtc ggcggcgccc agtcgatcca gacgggggcc gtcgggatcc
     5101 agtccatcgt ggcggggctt gcggccgccc tcgaccagat catcgccggc gcccagcaga
     5161 tcgagggagg tctgggccag gccatcgcgg gcatcggcct gccgtccgac gccccggacg
     5221 cgagcatccg cggcggtctg aacgccatcc gtcagggagc gcttcagctc gccgacggca
     5281 tggccgacgc tatcgccgga atcggcgacg aggacacgcc cgacacgctc ctcttcgggg
     5341 ccaacgcggc ctccgagggt gcggaccttt tgcgggccgg ctcggctcag ctgacgtccg
     5401 ggctgggcga ggcccactcg ggctcgggcg agctcacggc ggggctgaca cagatctccg
     5461 acggggcggg gcagctggcg tcgggcctgg gcgaggcctc cagcgggtca agccgcctgt
     5521 cgaagggcat cggagacgcg aaggacggcg cccagcagat cgagcagggc gtgtatctcg
     5581 tgaacgaggt gggcgtgaag gaggtctccg cctccgcgaa cgagacggcg gccgagctgg
     5641 accgcaccct gcagttcatg cacgcgcagg acgagcgggc ggcgaacgag tcgctcctct
     5701 acgggccgcc ctcgtcggat caggccgagg tcgtggtcgg gggatccgga gtggtcctca
     5761 cgatggacgc gctcgatgcc cggtccgcgg agtccaccgc ccggggcatc ggcgccctga
     5821 tcgctctggt gatcctcggc gggctcctgc tcgtgattcg gggcgtgcga cggacgccgt
     5881 cgcccgccta gggggagcca ggggggcggg ccgggcgctt cgcgcccggc gcgcccccct
     5941 cgacctgtca tgaccctgtt cctccagcag ctgttcgccg gtctcgccca ggggagcgtg
     6001 tacgccctcg tggccttcgg gtacgccctg atcttctcca cgtcgagggt tgtgaacttc
     6061 gcccaggggc acctgttcat gctcggcgcg atgactggcg tcgcccttct cgggatcggc
     6121 gtcccctacc tgctgatgat ccccctgacc atggcgatcg tggcggcttc gggcctcgcc
     6181 ctggagcgct ccatcacgct gccgctgagg gcgacacgca gcgcgtacac gtggatcgtg
     6241 gccacgctgg ccgccgccgt cgtgatcgag aacgtcgcct cgatgatctt cgggaaggag
     6301 ccccggacct tcccgccggc gatcgcgggg gtcaccagga tcggggatct cgtgatccag
     6361 catcacgccg tcgtcgtcct gggagtcgcc ctggccgtga tgatcgggat cgagctgtgg
     6421 cttgggagga cgatgatcgg gacggccttc cgggccacgg ccgacaaccc ggccccggcc
     6481 cggctctcgg gcgtgccggt gcctcggctc gtcatgctct cgttcgcgct gtccggggcg
     6541 atcgcggggc tgggcggcgt cctggtggcg ccgatcacgt tcgcggccgt cccgatcggg
     6601 ctgctcctcg gcctcaaggg cttcatcgcc gcggtgatcg gcgggatggg ctcgttccgg
     6661 ggagcgctcg tgggcgggct gtcgctcggg ctggccgaga cggtgatccg ccagcagctc
     6721 cccgcgggga tgggcggcac gatcctgttc ggcttcctcc tcctcacgct cgtcatgcga
     6781 ccgaccgggc tcttcgggag acgcctggcg tgagggcccg ccgagcgagc ttctggggag
     6841 gggtcgccct ggtgttcctg ggcgcgtcgc tcgtgtacct gcccgtcggc gagttctacc
     6901 tgttcctggc ctcccggagc gcctgcttcc tgctcgtcgt ggcggggctg aacctcgccc
     6961 tcggctacac gggcctgttc tcgttcgccc acgtggcgct gttcgcgctg ggagcctacg
     7021 cgacggcgat cgcggtgacg cggcagggcc tccccgtctg gaccgggatc ggcgcggcgc
     7081 tcctggtcgg ggcggtggcc ggcgccgtgc tcaccctggc cacgttccgg gcccgcgcga
     7141 tccacttcgc cgtggtctcg ctcgtgctgg tcttcgcggt ctccgagctc ctgagcgggt
     7201 ggacctcggt gacgggcgga gaggcgggca tcaccgtgcg ccggccggag ctcgcggggg
     7261 atgcgctcct ggcccggagg tactggctct tcgtcttggc ggtcgctgca gtctgcctcc
     7321 tggcgatccg gaacctggtg cgctcgcccc tggggcgcgc cttggtggcg gtccgcgaga
     7381 gcgaggaggc ggcggcgtcg gtggggatcg acccgttccg ataccgaacc gtggccatgg
     7441 ccttcggggg agcggtggct gcggccgggg gcgcgctctt cgcacacctg gacggcttcg
     7501 tcagccccga gatctcggga ttcgacgacg ccacgctgtt cgtggcgatg ctgctggtgg
     7561 gcggcgccgg agcgctctgg ggccctgtgc tcggggtggg tgcgttcctc gtgatcgatc
     7621 gggtcctcgt cgctctgcgg ggagcactcc cggggctgga tctggtcgtc tggcaggcgc
     7681 tgatctccgg gatcctcctc ttcctcgtga tcgtcttcct gccgcggggg atggcgggcg
     7741 cgctgacctc gcgccgccgg gaggcgaccg tcgccgatcg ggacgagcca ccagccgcgg
     7801 cggtccttcc cgcgccccgg atcccgtggc cggagggccc ggcgctcgag gtggtggggc
     7861 tgacgaagcg cttcggcggc gtccgggcgg tggacgacct cgacctcgtg gtggaggggg
     7921 gcacgatcca cgggctgatc gggcccaacg gctcgggcaa gaccacgacg gtggacctgc
     7981 tcaccggcgc cctggcggcc gacgcggggg agatccggtt cctgggtgag cgagtggtcc
     8041 ggccccgtcc ccacgccatg gccgcccgcg ggctggggag ggtgttccag cggacggagg
     8101 tcttcgccgc ggtcgcctcg ctggagaacg tcatggcggg gttccacctc gtggccgagc
     8161 ggggcctccc cgcgaacgtg ctccggaccc ctggggcccg ccgtcgggac gaggagctgc
     8221 ggcgcgaggc ggcgaccctc ctcgaggcgc tcggcctcgc cgagcgacgc cacctcctcg
     8281 ccgcgtcgct tcccttcggg gaccggcggc tgctcgaggt ggcccgcgcc ctggccgctc
     8341 gcccgacggt gctgatcctg gacgagcccg ccaccggcct gagcaccggg gagctgggtc
     8401 ggctcgctgc gctgctggct cggctgcgag agggaggggt cacggtcctg ctgatcgagc
     8461 acaacatgga gttcctgatg gggctctgtg accgggtcac ggtgctcgac tcgggtcgca
     8521 ggatcgccga gggcacgccg acagaggttc agtccgatcc caaggtggtc gaggcctacc
     8581 tgggcgagga ggcgggatga cggcgagccc gctgctgcgc atccagggcc tgacctgctc
     8641 gtacggggcg atcaccgctc tccgtggagt ggacctcgat gcgcacccca acgaggtcac
     8701 ctgcgtgctc ggcgtcaacg gggcggggaa gtccacgttg ctggcggctc tggccggcgc
     8761 gatccgcccg tctgcgggca gcgtgcgcgt gggggaggtg gagaccgtgg ggacctcgcc
     8821 ggagcgcatg gtggggctgg gggtcgtgct cgtccccgag ggacggcagg tgttcccctc
     8881 gctcaccgtc cgcgacaacc tgctcatcgg ggcgtatcgt ctccggcggg atcggtcggc
     8941 ggtcctcgag gggctcgacg aggtgcgtcg cctcttcccc atcctcggag agctcgcgga
     9001 gcgacccgcg ggcgcgctct cgggcggcga gcagcagatg ctcgcgatcg ggcgggcgct
     9061 catggggagg cctcgcgtcc tcatgctcga cgagccctcg ctgggcctcg ccccccgcgt
     9121 cgtgcgggtg gtgatggagg ccgtcgcggg gctcgcggcc gagggccgcg cggtcctgtt
     9181 ggtcgagcag ctcgctcgcg tggcgctgca gtcgtcgcac cggggctacc tcctggagtc
     9241 tgggacggtg gtcctggaag ggacgagcga ggacctgcag gccgacgacc gggtccgcga
     9301 gatctacatg ggagtgaagg gatgaggacc accgagccgc tggcccccga gagggaggcg
     9361 accgacaggt ccaggaccgg atcgtggatc cggctgatcg ccgcggtgct tggcctggct
     9421 gtgatcggcg tcgccgcccg gatgctggcc gacttccaat ggaaccgggt ggtcgagtac
     9481 cgctcgccgt acgtcttcga tctgccggcg gccccggaga cgccccggct caccgaccgg
     9541 gtgatcctgg tggtggtgga cggccttcgc gtggacgcct ccgagcggat gcccaccctc
     9601 cagcggctgg gcggggaggg ttcctcgctc gtggcgcgaa cggggctgcc gtcgttgtcg
     9661 ttccccgggt ggacgaacct ggcttcgggc gcccccccgg atgtctcgag cgtgaccacg
     9721 aactggtacg agggacgcgt gcgcgtcgac tctctgctcg cctcggcccg tcgcgcgaac
     9781 ctcaccaccg ccgtggcggg cggagaaggc tggcgccagc tcttccgagg gcagatcgac
     9841 cacagcttct acgtcccgga cgcccgaagg ttcgccgacg agcgcatcgg aagggccgcg
     9901 ctccgcatcc tggccgagga cgaccccgat ctcctgatcg tgcacctccc cgacgtggat
     9961 caccgcgggc acgagacggg cgtgaacggg ccctatcatg acgcggccct cggcgccgac
    10021 ggcgtgatcg gggccctggt cgaagcgacc gaggacaacg cggcgatcat cgtcacctcg
    10081 gaccacggcc acatcgagag cggggggcac ggaggccccg aagaggaggt cgtccggacg
    10141 ccgctcgtcc tgtggggaga cgggctggtc cccggggcga gcggcgccgt gtcgcatccg
    10201 gacgtcgcgc ccacgatcgc ggccctgctg ggggtggggc ggccggccca cgccacgggg
    10261 cagctccgcg tggagctgct cgacgccgac gaccagaccc gggcccagat cgagtcggcg
    10321 cacgacgaga cgctggcgcg cttctatcag gcggcgacgg gagcgctcga ggcctccggg
    10381 tctacggagg cggcgttcga gcgcgccaga caggaccggc accgtcacga cgtcctcgcc
    10441 aggctcccga tcgcgctggg agtggcggcc ctgctcgcga tcgcggtggc cctggccacc
    10501 cggggcctcg acgcggtggc gatcctcgtc ggctcgatcg ggttcttcgg gacgtgggcc
    10561 ggcatcttcc tggcccgcgg cctcagcttc tcgttctcgc acttcaacac cgaagagcag
    10621 gttgagccct tcctcgtggc ccggctcctg gacacgctca tcgcgctcgg cgtggcctcg
    10681 gtgctggcgg gcctcgtggc cggacggagg cagcgggcgg ggggcttcca gacggggctg
    10741 ggcgtggcgg cctggatcct gctgatcacg ggcatggcgg tggcgggatt cctggtcgtg
    10801 ttcgggtggg ggttcgtctg ggggctcccg aacctcgtgg ccggcttcgc ggagttcctg
    10861 gccctgcttg ccatgttcgg cgtgggcgtg gcggcctggg cggccggtct cgtctcgctg
    10921 gggacggccg cggtcgtggc gccgcggcgc gcgcgctgag gcgcgcgccc ctccgcgtgt
    10981 aggctggtag ccatggacga ggaacagcgg accccggacg tcatcgtgcc gggtatgacg
    11041 aaggacgagg acgactccca ggagatcgtc gaacaccccg cgaagctgct caggatcgcc
    11101 tcgatgatcc gggagctgct ggaggaggcc cggcactcct cgctcgacga ggggggccgc
    11161 aagcggctcc aggagatcta cgatcgggcg ctcgtcgagc tgaaggacgt cctcaccgag
    11221 gacctgcgcc gggagctcga cgcgctggcc atccccttca ccgacacccc cagcgagtcg
    11281 gagatccgcg tggcccaggc ccagctcgtg ggctggctgg agggcctgtt ccacgggatc
    11341 caggccgccc tctgggctca gcagatgcag gcccgccagc agctggagga gatgcggcgg
    11401 cggggccttc ccagcgggct tcccggccag ccgcaacagg ggcagtcccc gccgggccag
    11461 tacctctgag ttggacgagc tgcacgcctt cgtgggcttc gccatcgtgg gcggcttcgc
    11521 cctgctgagc ctgtggggcc tcatcacctg gctgatccgg agagggccgg gcaaggcctt
    11581 ctggtgggtg gtcggcacgc tgcagggggc cctgatcgtc cagctcctcg tgggcgtcgt
    11641 cctgctggcg ctgggagggg gacgggggat cctccattac ctgtacggcg tcgggttccc
    11701 cgcgctgatc ctggtggggg cgcactggct ggcccgcgag gccttcgccg atcggccctg
    11761 ggcgccgttc gctgtggggt ccttcttcat cttcgggctg acgctgcggg cgctcatgac
    11821 cgggctcggc atcggctgat cgggtcacct gctaccctgg ccgcatggca cgaaggaagg
    11881 gaagaaggcc gcctccccgt cgatcggcgg gcagacgaca gccgctcacg gccgagcagc
    11941 gggccaagcg gatggagcgg gccggccctc agaaggagga tgcccttcag gtcgagggga
    12001 cgatcatcga ggcccttccg aacgtgatgt tccggatcga cctcgacaac ggccacaaca
    12061 tcctcgggca catctcgggg aagatgcgca agcactacat ccgcatcctc ccgggcgacc
    12121 gcgtgacggt ggagctctcg ccctacgacc tcacgcgtgg ccgcatcgtc tacaggcacc
    12181 gctgatcccc cgggggcgtc cccggtgacc cctcgtcgca cgctggccct cggagcgacg
    12241 gtcgcgctcg tgctcctgtt gtggtgggcc tcggggctcg atcgctcctc cgcccctgcc
    12301 gaccgcttct tctcccagcc cgagatcgct cgggcgagcg agtaccggac tcccctctac
    12361 gcagcggcgg cgctgcgcct gtccctcgac cttgggctgc tggccgcgct ggccttcacg
    12421 ggcgccgggg cgtggctcgg gcgcctgcgg ggatcgcggt ggatccgcgg agcggcgctt
    12481 gcgatcgtgg cgatcgtcct gctgcgcttc gtggtccggc tcccgatctc cttctgggcc
    12541 ggctacgtcc acgagcattc gtggggcttc tctacgcaaa ccgtctgggg gtggctgggc
    12601 gacgccggaa ggtcggtcgg gctggcgctc gccgtctcga tccccgtcta cgtcgccgcg
    12661 ctcatcctcc tgggctcgtt cgtctggccg gtcgtcgtcg agccactctt caaccggttc
    12721 gagcctctcc gcgggcgggc ggctgatcgc ttcgaagacc tggcggaccg ggcgggggtt
    12781 cccgtggacg ccgtgctcgt ggcagacgcc agccgacgca cgaggaagga gaacgcctac
    12841 gtctcgggct tcgggggcac gaagcgcctc gtgctctacg atacgctcct gcgatcggcg
    12901 tcccaggagg aggccgaggt cgtcgtggcc cacgagctgg gccatcggcg aggaggccac
    12961 gtgcttcgag ggacgctcgt ggcggccctc gccggtgcgg gagcgatcct actgatcgcg
    13021 ctcgcggcgc ggacccggtg gctgcggagg atcagcgggg agaccggtcc ggacgacccg
    13081 cggatgcttc ccgcgctgct gctcggcatc tcgctcctca gcctcgcggc gctcccgccc
    13141 tccaacctcc tctcgcgctc gttcgaggag gaggcggatc gggccgcact ggcgctcacg
    13201 ggggaccggc gggcctacga agccgcccac cggggcctgg cgctgcggaa cctctccgac
    13261 ctcgacccgg gaccggtcgt ctaccgcttc ctggccacgc accccacggc gcccgagcgc
    13321 atcgcctacg cgctcgagcc ctgatcgacg gcgtcgagga aggcgcggac cgcgcccccg
    13381 agcgactcgg agaggtagcc gccctcctgc acgatgagga cggggggccc gagggcggcc
    13441 gccatcgacc ccaacgccct gaacgcctcg tcggtgaggg cgaaccgccc catcggatcg
    13501 tcctcgtgag cgtcgaggcc cagtgagacg acgagggcct ccggcgcgtg gtcggcggtg
    13561 cgggcgaagg cgcgctccgc ggcctgcagg tacacgtcgt cgccggtccc gagggggagg
    13621 gggaggttca cggtggcctc gcccccgtcg gcctcccgac ccgtgaagta ggggtactcg
    13681 acgtcggggt cggcgtggat cgagacgtag agcaccgtcg gatcgtcccg gaagatctcc
    13741 tgggtcccgt tcccgtggtg gtagtcgacg tcgaggatcg ccacggggcc ggcttcctca
    13801 cggagggcgg ccgcggcggc ggcggcgctg ttgagatagc agtagccgcc ggcgaaggtc
    13861 cggccggcgt ggtggccggg aggccggcag agcgcgtagg cggctcgctc gcctcccagc
    13921 aggaggtcgg cccccgtgag ggcgcagtcc gccgccgccg cggccgccct ccacgtcccc
    13981 tcgaggatcg gcgtggatgc gtcgaaggta tacgccccga cccgggcctc ggcgccccgc
    14041 ggtctcggcc ccgcgacgac gtcggggagg gcgaaggtgt cggccgcgag cggtccggcg
    14101 ccgccctcgg ccacccaatc ccgccacgcg gatgccaggt gatcgaggta ggccgggtcg
    14161 tggatccgct ccagcggcga tctcccgtgc tcggtgggct cgctcaccgg gtgcgcctcc
    14221 gagactgcct cgaggatccg gtccaccctc tcggggacct cgtaggcggg gaccacctcg
    14281 ccaagcacga actcggttcg cggggcgtga tcgcggtggg tgccggtggc gacgacgtgc
    14341 atcggagcag tctgtccggc gcggcggccg ggggcaagga aagacggaac cgcgagagtc
    14401 cgaagactcc cgcggttcca gagggatttg tacaccgagc ccaggcccgg gcgcaagacc
    14461 ccaaccccgc ttttgtcact ctttctcggt tcccgctgcc ggggcagccg cgttcggggt
    14521 ggttcgtccc gatacgacat cgctcccatg cagcccgacg gtgtacaaat acagtccatg
    14581 aacgagcaga gcctgcggga gagcaacggc atcctggcgg ctgccgccga tcgggcggcc
    14641 gtgggggagc tgatccccgc cacccccgcc gagctcgcgc gggaggtcgg ggtggacgat
    14701 cggctggccg tagccagggc cgtgcgcgcc ctgctggccc gggggcggct ggagcaggag
    14761 ggcgatcggt accgcctcct cgacacgagg cctctcgagc gcggcgagcc ggcaacggtt
    14821 cgtcgtccct cgaggagacg cgccaggagc cgggaggccg aaacgcagga cgaaggcttc
    14881 cccacctacg agcaggtggg gaaggcgatc gtggagcgcc tgatcgagct ctcggccgag
    14941 tcggcggagc tcagggcctc gctcgagcgg gcccgttccg agagcgaggc cgcccgccga
    15001 gaggcgctcg aggccacccg cgccgcctca cgggagcgcc agcgggccac ggatttggag
    15061 caggagtccc ggacgctgcg ccagcgcctc gagatgaccg agtccaacct ccggcgcatc
    15121 atcgaggccg cccagagccg gcccgcggcc cggctcgagg actccgacgc caaggcgatc
    15181 ctcgacgttc tctccaagaa ggacgcatga gaattccccc agacgaccga cggggccacc
    15241 acccgcaggg atgacgccgt tgggcgattg cgccgaggcg ccgccctctg catcctgaac
    15301 tggggaccgc tcttctgggg aaggaggttg tgtgccgggg ccgatcgtgg gttgggtgtc
    15361 cctcatcgct cagatcccat ccgacttcct cgggctctcg gcctcccagt ggttctgggt
    15421 cgccatcgtg ggctggtccc ttggcgtcgt cctcggcgga ctgctcgccg cccgggccgt
    15481 ccagggagct cccgggcagg gagaggtgag cccggagcgc atcgcgctcg acacccccgc
    15541 gatcctccgg gccgtggcgc ccgacgagga cgcggcgggg ggcgtgcttc gatggctgct
    15601 gggggaggtc gatgccgatg ccgtggctca cctcgagctc tccccgcttg gggagaccct
    15661 gcggatcgag cctcgcggcc tggagggggc gtcggtcgct cggctggccg agctcgcccg
    15721 gtcggctctg ctgggctctg agcctctcgc cgagggcgtc ggcgtcgcgc gttgggtcgg
    15781 cgccggcggg acgaacgcgc tcgtggctac cgggacggcg atcccggggc acgaggagcc
    15841 gctccggttc tcacgctacc tgctcgaatg ggttcgatcg gcggagcgcg ggcgtccacg
    15901 aggggacctc gaggaccgcc ttcgcgaggt ctcgggcgtg acgtgggtcg aggtcgtggg
    15961 ccgatcggct cggctcctgg cggaggacga ggcgagcgcc gaggtcgcgc tggccgaggt
    16021 ggcccgacgg ctcgagggca caggtgtggc cccggtctgg atcgacgcgg cggagccccg
    16081 gccggacgtg cccaccggcg gccctgtggc cggggcggtg ggggggccgg aaccccccgt
    16141 cgtggccatc ccggaggccg agcggtccga ggcccagcgc atccccaccg agccgatcgg
    16201 catgaaggag tccctcgtgg cgtcggcgat cggcgcgcag gagccaagga tcaggctgct
    16261 cgacgtcgct ctccgagaga acggcgaggc cacggcggac gtccgcgtga gctggaacga
    16321 gcaggagatc cggggacggg gccacggacg cgccacgcgg gccggccgat ccttcgccgc
    16381 ggcgcaggca gtggccgacg cgctgcgccc gctcctcgac acggacgtcg tgatcgaggg
    16441 cctctacgtc gccacgacga aggagggcct cgacgtcctg atctccgagg tcaagatgga
    16501 ggggcagcgg tacgtgggag ccgtcctgga gcgccatgac cggcccgact ggacgggggc
    16561 ccgcgcggtc ctcgacgcgg tcaaccgtcg gctggtgcag gtcgccggcc gcagcggtcg
    16621 gatctgagcg ctcacctccg ccgtcggatc agcagcagcg tcgccccccc gagcaccgag
    16681 gccccggcca gggcggcgac cgcgaccccg aagggcaccg tcgggtcgcc gaagtcgctg
    16741 ggctgggggg gtcgctgcgg cgccccctcg gagggcgccg gagcgacccc cccagggctg
    16801 acggtgacgg agatcgctcc cgtcccgacg acctgctcgg cccccgtctc gaggtccctg
    16861 acgagcacgt ccccgttccc gcggacgacc gccatggtcg atccctccac atcgaacgag
    16921 atcgccctcc gggcgatggt gcgcccgtcg agggtgcgca gggggccggg ccgccccacg
    16981 gcgggcgatt cgatgatcgc cacgccttcc ggcgtgtagg agacgtccag gatcgctcgc
    17041 ccggcggcct cgtggaggac ctcggggccg tcgggacgcc cgtactcggt caccgtggtg
    17101 aacccctcgg ccctggtcac gaccagcagc cgctcgcccg cgtcgtccca gtccatcggg
    17161 tggaacgagc actcgcagta gccctccacc gactcgagca cggtctcctc ggggcctccg
    17221 agcacgacga gctggggcgc ggcgacggcc ccgccctcac gccacgacgg accgatcagc
    17281 tcctccatcg gccgcaggga ctcggcgaac tcgaccgcgt cctcgggccg tccgaactcg
    17341 cccaccgcgt agctcgagcc ctcctcccag tcgaggaagc aggcagaggc tccgcacgag
    17401 aaggagccgt cggtgccgcg gacctccacg gggagggcat cgggcagagg ggcgacgggc
    17461 cggggggagg cgccgacgaa gacgttcacc tgctcggcgt gggacagcac accgtccgga
    17521 tcgttcgtga agcggagctc gtagcggcgc ccgccggcgc tggctaccgt gtgggtcgtc
    17581 cccggcggcc tccggggcgg ggcgagcacc gtgatcgacg tcgtggcgag ctcgtcgagg
    17641 gccccccgaa ggtgcctcgg tacgtcggac ccggacgcga cccggcgctc caccgcgacc
    17701 cggccgcgcg cggccgcgat gcccgagagg gatccggtgg ctcgggggcc gtccgccgcg
    17761 ccggacccga tccgcaccct gaggagctcg gtcgtctcgc ccctgcggac cagggcgaag
    17821 acggcgcccg cccctcccgc gtcggcgccg acgacgccgg cgtccaggag ggtccgctcg
    17881 cctccgccgc cgggacggac cgcgatgagg gagcccgggc cgacctgctc ccctgcccgg
    17941 acgtaggcga tccaccgcgc ctccgaggac cggccctcca ccggggacgt agtcgcaagg
    18001 agggcgccca gcaggattgc gagggcgggt cgccgcacgc cgccgagcgt acacgctagg
    18061 gtggggcgtg tcgccgaagg atccggacat ccgtcccgac cggccggggt cgatccgtgc
    18121 gatgttcacg gacctcgctc ccgactatga ccgcttgaac accctcctca cgttcggtcg
    18181 tgtgcgctcg tggcgccgga ctctggtgcg ggcggccggc gtccggttcg gggatcgggt
    18241 gctcgacgtc tgcgccggca ccggcgcctc cttggctgcg gtcagggagg ccgtgggagc
    18301 gcggggggtc gccgtaggcg tcgacttcac cccggcgatg ctcttgcgag cggagggggt
    18361 gcgcgtgctg ggggacgccc tgcgtctccc cttcctcgac cgatccttcg acgccacggt
    18421 cagcgccttc gccctgcgcg acgtcgcgga ccagccggcc tgcatcgccg agatgtttcg
    18481 agtgacgcgc cccggcgggg gtgtggcgat cctcgaggtg gggcgccccc accgtcagcc
    18541 gatgcgggcg ggattcgatc tgtggttccg gggcgcggcg ccgaggctgg gcgcggccct
    18601 cgggcagggc gaatcgcaca ggttcctggc ccgctctatc ggctacctgc cggaaccgga
    18661 ggcgctcctg gggatgctgc gggacgccgg gctcgtgggg gtccgggcgc gggacctcac
    18721 cctgggcgcc gcccgcctgt tcagcgggtt caggcccgcg tgaggtcgct gtcctcgatc
    18781 aggacgggct cgtcgctcca gagcgtgaac tcgaggtcgt cctcgatgac gcggagctcc
    18841 cagacgccct cctccggccg ggtacgggtg gagaggggat cccccgaagg gaggcgggtc
    18901 tcgctcacga gacctcctgc cgccggagga gggccatctg ggatgtgccc cggctcctcg
    18961 gtcgtgccgg gacggaccgc gcgcgctcat ccgacgaccc ggcgcggagg ccgggaccgg
    19021 agacgctccc ccgtgagggg atggggacga acgcccgatc ctgcacggca ccgaggatgc
    19081 ccccggggcg tgtcgttcgc ataacgggta tatgacgatt ctgtgacgat gaagtcagtc
    19141 cgcttccgac tcgtccctct cgagcggcgc gaagcgctgg taatgggccc tcgtcaccga
    19201 cccctgcacc acctcgtccc ggccggtcag cacgtcggcg gggctgccct cgatccggaa
    19261 cagcaccgcc tccaccggag gcgtctccgt cagcgtgtac acgacctggg ccacgcgcag
    19321 ggccaggcgg tggcccgggg cgctcagctc gaactcgtcg gagaggtcca ccgtcgccac
    19381 gatcccgtcg acctccaccg agagcaggcg ggtgccctcg gggatcgcgg tcttccaggg
    19441 gctggagggg ccctgcaaga gcgcctccat cgcggccgcc gggagggacg gagcctccgc
    19501 cggcagctgt ctggggaccg cgatgaggcg cccgccgagc acgaagtaga cgatcgtgct
    19561 ctcgggtagc tcggtgaggg tctcgggccg gggggatccg aagacgtcct gcggcagctc
    19621 ggcctcgggc acgaccgtca cggcgggctc gccgcacgcc gccagggcca gcgcgaggat
    19681 gacgacccgc ctcatgcgcc cgtccgggag acgcgcggga ggcggaagag gaacctcgcg
    19741 ccggcgcccg ggctgctctc tacctcgatc tcgcccccgt ggagcttggc gttctcctgg
    19801 gcgattgcca gtcccagacc cgagccccgc ccatcgaggc ggggccggga agggtcggcc
    19861 ttgtagaagc gatcgaagat ccgaggcagg tcctgccccg cgatcccggg tccccgatcc
    19921 tccaccgcga gggtgatcgt gccgttggcc gcgccgaccc ggacggtgac ggggaggtcg
    19981 ccgtgggtga gtgcgttgtc gacgaggttg cccaccactc gctccaggcg ccgcttgtcg
    20041 gcgagggtcg tgagcgtttc ccctccgccg ttcacctcga cgcgcacctc gccgtccgcc
    20101 gccctcgacc gccgagcgac cacctccctg acgaggtccc cgacgttcaa cggctccagc
    20161 gagagctgga tcggccccga ctcgagccgc gagatgtcga ggaggtcgtc cacgagcctc
    20221 cgcaggtagt cgaggtcggc ggagagcagc tcgacggcgc gctcgtgctg ctcgggcagc
    20281 tcgtcggccc gctccagcag gtagtcggcc gaggcgcgca gggtcgtgag cggcgtgcgc
    20341 agctcgtggg agacgtctcc gacgaagcgc cgctcccggg cgatgcgctc ctcgagggcg
    20401 gcggccatct cgttgaagga gtgggccatc gtcccgatct cgtcacgctc ggcgaccggg
    20461 agccgggtgg ccaggagacc ctcggccacg cggcgcgcgg cgtcgctggc cagccggacc
    20521 gggtgcacca cgcgccggct cacgcgcacc gcggcgatcg ccgccagcat gacggcgccc
    20581 agggacacca tgagcaggac cttccagaga agggccaggg cctccgccac gtcgcgcacc
    20641 gggtagaaga agaatgccgt gatgtccgac cccgggatcg gggagccgaa cacgaggaag
    20701 ggctcgtccc gctggaaccg gtaggcgacc tgtccccggc gcacctcctc gagcacggga
    20761 gcggggaccg atcgctgggt gatcgagagg gtggagggga accactgctc gcccttcatg
    20821 agcagcacgt cgaaggaccc gcgggcttcg agcagccccg ccagctccgc cagcgacgcc
    20881 tgctccggct ccacgtatcg gacggcgaaa gcgatgttcg agaacgactg ctccgtggcc
    20941 gcctcgatcc gctggttcac gacgtagcgg tcggcgaggt agtaggtgct gccggcgacg
    21001 agggccgaga cgcccagggc ccccccgatg aaggccacca tgatcctccg tctgaggccg
    21061 cgtgggcgcc gccgccgcgc catcacccca ccaccttgta gccgatcccg cggacggtct
    21121 gcacgatctc gggccgccgc ggatccgcct caatcttgga ccgcagacgc ctgatgtgca
    21181 cgtccacgat cctggagtcg ccgaagtagt cgtactccca cacctggctc agcagcatct
    21241 cgcgggtgaa cacctggttg ggcctggagg ccatcgttgt gaggaggcgg aactcggtct
    21301 tcgtcaggtg gatctcctcg cctgccttgg tgacgctggc cgcgtcgggg aggatctcga
    21361 gatcgcccac ggcgatgcgt cgctgcccgt tgccgtcctc cggccctcgc gagcggcgga
    21421 gcagggcgcg gatcctcgcc acgagctcct tggtcacgaa gggcttggtg acgtagtcgt
    21481 cggcgccgga ctccaggccc acgacgacgt cgacggtgtc ggtcttcgcc gtgagcatca
    21541 ggatcgggac cggggagcgg gtgcggacgt cccggcagag ctcgaacccg tcctgcccgg
    21601 ggagcatcac gtctaggatg aggaggtcgg gctcctctcg ggccaggaga tccgaggcct
    21661 cctccgcgga gccggcgggc aggacgcgat agccctctcc ttcgagcgcc atgcccacga
    21721 tctcccggag acgagcgtcg tcgtccacca gcaggatgag ctgcccggtc acgggcccat
    21781 gatgccaccg ccgggccgac cgggtggggg tcggtcagcc cgtcagcacg ggatggccgt
    21841 ggtcctcgat gaacgcgggc acgagacgtc cccgcatctt cccgctcggg gtgacgacca
    21901 ccctggccac ggcggtggtg gcgccctcat gcgagaagct gggccagacg aagttgccga
    21961 ggccgtaggc gatcggcctc cccttgtagt gctccagcgg ctgcaggcgg tgggcgtggt
    22021 gcccgaagat ggcgtcggct ccggcgtcga tcatcgcgtg ggcccgctcg acgtcgtcgg
    22081 gagacggggc cgtggagagc tcggtgcccc agtgaacggt cacgatcacc agatccgctc
    22141 gacgatcggc cgcccggacg gcacgggtca tgttctcgat cgagtcgccg tcggccatcc
    22201 cgggggcgtt ctcggcggcg atccaggccg gggaggggat cacgcctccg aacccgagca
    22261 cggcgatcct ccagcccttc gtctcgatga tgaccggcct ggtcgcctgc gcgacgttcc
    22321 tcccgacgcc gacgggtgcg atcctcgcct cacgcaggcg ccgccgggag tccaggagcg
    22381 cctccggccc gtagtccccc ccgtggttgt tggccaggtt cgcgacgtcc acgccggccc
    22441 gccgggcggc cggcagggcc gcggggtcgc accggaagct gaactccttg ggcaccggcg
    22501 tccccaggtc cgagacgggg cactcgaggt tgaccacggt gaggtcgtcc tcgcggaaga
    22561 ggtcttccag ccccgaccag gcgtagccgt agccctgcgt cgagagattg gggatgtagg
    22621 aggggtcgag gctcacgtcc cccgtgccgt ggatcatgag ccgccctctg gacggaggcg
    22681 tgggcgccag ctccggcgtc ggagccgacg tgggagcgtc ggagggggag ggcgcggggc
    22741 tcgcgatcgc cgtctcgggg gccgtgggga gggcctcgat cagctgggtc cggaggaccc
    22801 accccgcgcc gaccgcgagg agcacgacga ggggccccag cactgggccg acggaccttc
    22861 tcgtgcgccg gtgacgtccc tccaggtcgc cgtcagccta ccgggaaaaa gcggcccgat
    22921 ctattaacgc agatgcggta gctgccgata ccaggagcga ggctccccgg cgcccgccgg
    22981 ggaccgtggt gcgtggcggc ggctcgagtc gggtcccccg ggtcgccgcc cgcacccatc
    23041 ttcagtgctc accgtgcatc tcgtggacga tccgttcgcc ctcctcggcg ctcagccggg
    23101 cgtcgaggat cgggaggtag atctcctcct ccttggcgaa gtggaccgct accagtgcgt
    23161 ggagcccgta gaggatccgc cggagctcca tccaaactgc gtcgccgggg tcgtcttcca
    23221 gccgggggag cagatcgccg aggtcacctg tgagcgtctc gacctcgacg tggtcccggc
    23281 tcatcgtcgc cgcggcgccg ggggcgccca tcgcctcctc gaccgccggg tacagcacgc
    23341 ggtcctccgc cttcgcgtgg ggcaggaggt gctcgacgag gaagcggtgg gcgtcctcgg
    23401 cggccttcct cgcgtcgtcc ccgggcgcca ggtccgccgc gcgccggagg gattggatgt
    23461 gggggaggag gtgtcggtgc tcctctcgga tgggatccat cgggttgctc atgggcagtc
    23521 gcctcctgtc cccaccgtac ctcaggggtt gtacggggct tccaggcccc ggtcgggccg
    23581 gaagtggtcc aggctgacct cgacgtagac ggcgcgggcc tccacggcca ggtcctctcc
    23641 gacgtaccca gtcatgcggg cgtggatcct tcgtccctcg accttctcga gggtgccgcg
    23701 gagctcgagc gtcgctccga ccggcacggg cctgcggtag tcgacctcga ggtggccggt
    23761 gaccgccggc gtccgcagca ggtagaggag gtagcccatg ccttcgtcca tcgccgccgc
    23821 gaccacgccg ccgtgggcga gcatcggcgc gccctggtga tgctcagtga cggtgaacga
    23881 gccgcggacc tcgcgaccgc cgccttccat ccgcaggtgc aggccggtga ggtgctcgcg
    23941 gccgcagccg aagcagtggt cgtagtggga ggggagcggg cggaacccct cctccggagg
    24001 tgtcgccatg cgcggagacc ttaggttccc ggacccggcg cggtcaacct gaaggaaccc
    24061 tcaggcgcgg ctcaggggcg cctcaggcgg ggaggcgacc atctcttcag acaccgaccg
    24121 aaggaggtca ggatgcggaa gggaaggttg tggaagctcg tggcgggagg ctcggcgatc
    24181 ggcatcggcg ccgcggcggg gatcgcggtc gccgggacgg acgcgcaggc tccccagccg
    24241 gtcgagctgg gcgagacggg cgagacctcg acgctcgacg tcgaggggac gcccgtgacg
    24301 gtggcttcgc cgagcccgct cggcgactcg ctggatgacg ggaacgcggg ggcgtcggtc
    24361 gacacctccg ggagcgcgag ctctgcggac acctcgggga gcgccgacac gtccgggtcg
    24421 acggacacct cggggtcggc ggacacctcg gggacggaca cgatggacgg ggccgactct
    24481 cccgacgacg acaacagcgg gccgggcagc tgatgccgga gcgcggcatc ccgcgggacg
    24541 aggcgctgcc gggcgccgag gggctcaccg ggcccctcgg cgcccgggcc gtggcgtcgt
    24601 tcctcgaggc gagggggtgg cgcgcgacgg cctcgcggcc cgtgcaggcc ctctaccgtc
    24661 cgggccgcga ggccgtcgcg cgccaccggg tccaggccga gggtcccggc ggcgggcgcc
    24721 gtgtgctgac gatcgtcacg gaggtccggg cccgccgccg ggacccggtc cccccgccgt
    24781 cctggttcga ggaccgcttc ggcgtccccg aaccggtggg ggaggtgggc cgcttcctcg
    24841 tgtgggcgtt ccccttcgac ccgggacttc ccgcgctgcc ggaggccgct gacgggcgat
    24901 cggtgcggga caggctacgg cacgccgctc cggctgcggt cgcggtccag ccgctgacgt
    24961 accggccccg gcggagggcg gtgttccggt accgcgtcgt ggggagtgcg agcgaggtgc
    25021 tgtacggcaa ggtcctgcgc cagcggaagc tgcggcgggc ccgggaggtg atgcgccggg
    25081 tgcgcgggcg gcgggtccgg ctcggcacgg cgacggaagc cgagggcctg ctcctctcgc
    25141 aggccgtccc cgggcgctcg ctccgagacg tgctcgtgcg cggcggcccc gtgccgagcc
    25201 ccgcacgcgt cgcgggactc ctcggttcgc tggaggcccc cggggtgggg ggcgaggcgc
    25261 ccgatcccgt ggccgacgcc gggaccaccg cctcgctcat cgagcggatc gtcccccagg
    25321 cagctggccc ggccgcccgc gtggccgacc gtgtggagcg tggagctgcc gggaccccat
    25381 cacctgttgg aaccgtgcac ggagacctct acgagcacca ggtgctggtg gacgagggct
    25441 tctcgctcgg gctggtggac ctcgacgacg tcggccccgg cgacctggcg gtggacgccg
    25501 ccaacttcac cgcccacctc gtggccctgg ccatggccgt tccggcggcc gcccggcggc
    25561 tcctggccta ccgttcgatc ctggtccccg cgttcgccgc cagactggac gtggacgtgc
    25621 gggatctcgc ctggcgggag gcgctctcga tgctgaagct gtccgtggga ccgttccgcg
    25681 ttctccaacc cgcttggcag gctgaggtgc aacggcgcgt cgaggtggcg ctacgcttgt
    25741 cccaggggtg gtcgtgagcc gaacccacgt tctgctgatc gaggacgacg cgcggatccg
    25801 ggacatcgtc gagcaggggc tcggagcgcg cggattcgtc gtgaccagcg cgcctgacgc
    25861 aggcacgggc gtggacctgg cggggaagct cgacgtggcg ctgatcctgc tcgacctcgt
    25921 gctgccagga cgccaggggc tggacgctct ggggaccctg aaggagctca agcccgatgt
    25981 gcccgtgatc gcgctgaccg cgctggacga cacgggatcg aaggtcggcg gcctcgatgc
    26041 gggcgtcgac gactacgtga cgaagccctt ctcggtggag gagctggccg cccggatccg
    26101 ggcccggctc cgagaccgcg acgacggcga gggcgtcatg aaggccggcg ccctcaccgt
    26161 ggacctcgcg gcgcaccgcg ccgtgctgca ggggcgggag gtggcgctct cggcgcggga
    26221 gctctccctc ctcgccgcgt tcatgcggca ccgggggcag gtgctctccc gcgaccagct
    26281 gctgcgtctc gtctgggaca tggacttcga tcccggctcc aatgtggtcg aggtctttgt
    26341 ggccgccctc cggaggaagc tcgggggcgg gatcatcgag accgtgcggg gcaccggcta
    26401 ccggttcgtg ggacctcagg aggaggcatg agggtcgcgg tcgcggcgtt cctgggagct
    26461 ctgctcttcc tggcgggggc cgtgtacgga tcgggcgcgg tcgtccctga gagccagccc
    26521 ccgcaggccg tcgtcctggt ggacgacgcc tccccggcgc ccgccgcctc acccgagcct
    26581 acgcccactc cgacgaacga cgacgacgag gtggaggagg tcgagcacga ggtcgaggag
    26641 gacgatgacc actccgggcg cgggcggggc cgcggcggcg acgatgacga ggaggaggag
    26701 gacgacgacg actcctcggg cccgggcgat ggcggctagg cggtctctcc cgctcgggcc
    26761 acgcctcgtc ctcacccacg tcctcatcgc aggggccgtg ctgctcgtgg tcgctgcgct
    26821 cgtcttccac ctgacccggg tgcatctgct gcgtgggctc gacgcccggc tgtcgatcgc
    26881 cgtccagtcg ttccagcagg gcccggctcg ccaggtcgcc cgccccgccg acctccccaa
    26941 cgcggccaga agctggctgg ccgtccacgg gttcacctcc aaggaggtgg cggcggtccg
    27001 gacgtcggcc gggccggtgc tctcggcggc gggcggcatg ccggtgtccg acctcccccc
    27061 agaggtcctg gacgctcgct cctcgcgctg gtgggagatc gagatgtccg gcgagccggt
    27121 cagggcgctc accgtgcccc tcctgctggg ggatcgaacg gtggggaccc tcgtcgtggg
    27181 ctcctcgcgc gccgacatcc aggccacgct cgatgcgctc gtgcgcggca tgctgatcgc
    27241 ggcgggcttg gggctggctc tcgccgccgg gctcggcctg gccgccgtcc gccggacact
    27301 ccggccgctc cgccgcatga cgatgaggac cgacgagatc gaagccacgg gcgacctctc
    27361 gctgcgcgta tcgggcgacg ggccacggga cgaggtgggc cgtctggccg agtccttcga
    27421 cgcgatgctg gagcggctgg atgaggcgtt ccgctcccgg cagcgcttcg tggcggacgc
    27481 ctcccacgaa ctgcgcacgc cgctgaccgt ggtgcgggga cagctcgagc tgctgcggga
    27541 tcggctggag gccgagggcg accgagcctc ggtggggctg gcgatggacg agatcgaccg
    27601 catgcgccgc atcgtcgagg agctgctgct gctcgcgcgg ctggacgagg ggctccccct
    27661 cgagcggtcg ccggtcgagg tcgagctcct gatgcaggag gcgctcgtcc gggggctggc
    27721 gggggagcgc cggcgggcct ccgtggacgc cgagtccggg ctcctggccc tggccgaccc
    27781 tgagcgactt ctccaggtcc tgacgaacct cgtggcgaac gccgtcaaac acgccggcga
    27841 ggacgcccgg atctcgctcc gcgcgcggcg cgcgaacgac cgggtcgtcg tggaggtggc
    27901 cgacaccggg cgcggcatcc ctcccgacga gctgccccac gtgttctccc ggctgttccg
    27961 aggcacgagg gcccggggcg aggccggcgc cgggctcggc ctctcgatcg cgtcctcgct
    28021 gctggaggcg atggacggac gcgtccgcgt ctcctcccgg gagggggccg gcaccgtctt
    28081 caccgtggag ctcccgctcg cggcccccgc cacggctccg gccgaggcct agggcctttg
    28141 ctaccctcgt gggtcatgac cacgagacca ccacgccgac cgggcgagga gcccaccgct
    28201 gttctcacgc gggacgcgta cgagcggctc cggggcgagc tggaccgcct ccgcggcgag
    28261 ggccgtcggg agatggccga gcgactggag cgcgcccgcg agcatggcga catccgggag
    28321 aacgccgagt acgacgcggc caaggacgcc cagggcctga tggaggcccg gatccgcgag
    28381 atcgagcgcc tcctccgcga cccggacatc gtcgagggcg ccgcgtccac cgacgcggcc
    28441 ggggcgggtg ttctcatcac cgtccgcccg ctggacgacg aggatgacca ggaggagcgg
    28501 ttcctgctcg cggcctccaa ggaggagaag gccgaggggg cccgtaccgt gtcgctctcg
    28561 tccccacttg gggaggccct ggccgggaag aaggtcggcg agaaggtcac ctatgaggcc
    28621 cccgggggaa ccttcgccta cgaggtcctg tcgctcgaga tcccgtgaac gtccgcgtca
    28681 accggcgcgt cacgatcccc gcctccgagc tctcgttccg gttcacaccg tcgggggggc
    28741 cgggcggcca gcacgcgaac cgctcggcca cccgggtcga gctgatctgg aacgtcgagg
    28801 agagcgcggc gctggggccc cggcagaagg cgcgcgtccg ccgagcgcta cggggaagga
    28861 tcgacgcgga gggcaacctc cggctggtct ccggcgcgga gcggtcgcag gcccggaacc
    28921 gggccgacgt cgtggagcgg ctcggccgcc tgctggaggg agcgctcgcg gtgcctaagc
    28981 gccgcgtggg gacgaagccc tcggcggcgg cgcgggagcg tcgcatgaag gcgaagaggc
    29041 ggcggagcga ggtgaagcgg ctccgccgga cgcctcccgt ggagtgaccc ggtccgccgg
    29101 ggcacggtac gctcggtccg tcatgtcgct gctgatcggg ggcacgctcg cgctcctgtt
    29161 cctgcctgct ccctggtcga tcgtcgtcat cgtgggcctg gcggggttcg aggtgctgga
    29221 ggtggccttc tggctctggc tccggaggca gcgcccccgc tcggggcacg aggcgctcgt
    29281 tgggaggcac ggtgtgcttg tcgagggcgg ccgcgtccgg atcgcgggga cgacctaccc
    29341 cggccgggtg ctcgagggtg tgcctgggga cccggtgatc gtggaggagg tcgacgggat
    29401 gatcttggtc gtccgccggg gcgagcctcg gtacgaggag gagatcgcgt gagcgggcac
    29461 ccgctcgagc cgacgggggc cgagctccgg gcgatggtcg aggaggcggt gtcgtacctg
    29521 gcatcgttcc tggacgagct tccgggggcg ccggcggccg gcgcgcccga caaggaggcg
    29581 ctcgccgaga ccctccgcga ggaccacccg ccggccgccg gcgcccccct cgccgacgcg
    29641 ctctcggtga tccgcgaggc cgtgccccgc gcgttcgaca cgaccggt
//
LOCUS       DASUTZ010000219         5028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1501815, whole genome shotgun sequence.
ACCESSION   DASUTZ010000219 DASUTZ010000000
VERSION     DASUTZ010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5028
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501815"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VF097_09555"
     CDS             <1..549
                     /locus_tag="VF097_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004008131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX6263079.1"
                     /translation="PKGKRINEIIAETGADVDIHDDGTVFIGSKEGEGAERAKEMINA
                     IVNPVMPEVGEVFEGKVVKTTTFGAFVNLTPAKDGLVHISKLGRRGKRVENVEDVVNV
                     GETLTVKVRDVDQQGRISLEPVWGDEEEEAGSEEASAEEGRRDDRPREERPREERGER
                     GGGGGGERRRRRRRRPEGDRRE"
     gene            559..1821
                     /locus_tag="VF097_09560"
     CDS             559..1821
                     /locus_tag="VF097_09560"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX6263080.1"
                     /translation="MIGRTEYASGLRVVTERMPGVRSVTLGVWVPVGSRDEPARTAGA
                     SHFLEHLLFKGTKARSAQEIAEAFDAVGGDLNAFTGREQTVFYARVLDRDLPMAVDHL
                     TDMIERSLLRSRDVEAERQVILEEIHMTEDAPDQLVHDDFARILWGGHPLGRPILGTR
                     ETIEAMGRDQIRRFWKRHYVPANLVFVAAGNVEHEALTGLIESLVGTGAPVSGRRSTV
                     RDGGTPPRPSGIWDVRRRKTEQAHIAYGTAGLARRDPRRFAFGVVNNALGEGMSSRLF
                     QEVREKRGLAYSVFSYHAMYAETGLFCVYAGTTPVRATEVLRVIRGEIEDIAEKGLGP
                     DELERAKGHMKGGLVLSQEDTSSRMNRLGKSEVAHGEILTVDEVIERIDAVTGEDVRD
                     VAADVLGRAPSLAVIGPLGKDALREVLT"
     gene            1818..2606
                     /gene="dapB"
                     /locus_tag="VF097_09565"
     CDS             1818..2606
                     /gene="dapB"
                     /locus_tag="VF097_09565"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017981773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX6263081.1"
                     /translation="MIRVGVIGACGRMGTMASRAVIDDPELSLVAAVHPSRAGEDLAV
                     AVGHPRAEGRVSAQLEALVQADAEVAVDFTRPDVVMDDVRFGCTHGIHMVVGTTGVGP
                     DEQASIREWLKDSDANVVVAPNFSTGAVVAQRLAEEAARHFPAAEIIELHHEGKVDAP
                     SGTAAATARRIAAARGEAWKGPGEESVEGVRGGAVEGIPIHSVRLPGLVAHQEVLFGG
                     QGQTLSIRHDSTDRSSFMPGVVLAVKEVARRPGLTVGLESLLEL"
     gene            complement(2634..3164)
                     /locus_tag="VF097_09570"
     CDS             complement(2634..3164)
                     /locus_tag="VF097_09570"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HEX6263082.1"
                     /translation="MRYLELRRHADNEGDALTEAGKATAERVGRSMEGPFDGVFTSPA
                     KRAANTAAWFLRGMGQQLPQLHGVTDGLTSAEEERWREAGSRTGTWRVDDIEREDPEL
                     VREELPRLADAILQMFAAIRDGQRALVVGHSPFLEAAVYALSGTVLDPLGKCEGVLLG
                     HDGERVTVEGDLRLPT"
     gene            complement(3164..3478)
                     /locus_tag="VF097_09575"
     CDS             complement(3164..3478)
                     /locus_tag="VF097_09575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HEX6263083.1"
                     /translation="MKAHIWLRKKDGMSDEDFKDYWLTTHGPIAGDGYEHLTGYTVAL
                     VTRVPEGQEKPYDGVAILEWADRDGFKADMASPVAKESTDDLASFTSAFGLLFVEEHT
                     VK"
     gene            complement(3543..>5028)
                     /locus_tag="VF097_09580"
     CDS             complement(3543..>5028)
                     /locus_tag="VF097_09580"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HEX6263084.1"
                     /translation="AIVSSNVDLPIDYCDGGSDPTTIRVPAGRFRLSVGPAGDNAGLT
                     GDLDITDDVVIRGAGMGRTIIDANGLDRVFDVDTGFAGILVVLRDLTITGGSVTGTSG
                     GGIWNNGILVLKNVEVRGNESLDAVTGGGGGIWNYDTGILSVVESRIVGNRTTGSGTG
                     GGLRNRGTASVFDTLIKGNRSRDGGGIMSFPTPATLTVRDSALVGNVAFDGDGGGFNN
                     NNGATATFENVTVSKNRAARFGGGIYHFSGSSSFSHLTVADNRADFDGNAVGDGGGLA
                     VSIGTVELRASAFGRNKVGSGAATSDCSGGVQIQTLGGNIERGPNECNLLPLSGDVLA
                     NPKLGKLRNLGGPTPTHALAQKSRARGLVSASDCAPTDQRGVPRRGRCDAGAYELAAC
                     AGVVVNRVGTKGRDVLRGTRGPDGFLALSGNDVARGRGGDDAMCLGGGRDRAFGGAGT
                     DVLRGHGGNDVLKGQAGNDALFGGPGRDRCVQGPGTGRLRGCER"
BASE COUNT          706 a         1753 c         1832 g          737 t
ORIGIN      
        1 ccgaagggga agcggatcaa cgagatcatc gccgagaccg gggccgacgt ggacatccac
       61 gacgacggca cggtcttcat cggctccaag gagggcgagg gggccgagcg ggccaaggag
      121 atgatcaacg cgatcgtcaa cccggtgatg cccgaggtgg gcgaggtgtt cgagggcaag
      181 gtcgtgaaga cgacgacgtt cggggccttc gtcaacctca ccccggccaa ggacggcctg
      241 gtgcacatct cgaagctggg acgccgcggg aagcgcgtgg agaacgtcga ggacgtggtg
      301 aacgtcggcg agacgctcac ggtcaaggtc cgcgatgtgg accagcaggg ccgcatctcg
      361 ctcgagcccg tctgggggga cgaggaggag gaggccggct cggaggaggc ctcggccgag
      421 gaaggccgac gcgacgaccg tccgcgggag gagcgtccgc gtgaggagcg cggcgagcgt
      481 ggtgggggcg gcggcggcga gcgccgcagg cgccgccgcc gccgccccga gggcgaccgc
      541 cgggagtagc ccaccccggt gatcggccgc accgaatacg cgtccgggct ccgcgtcgtc
      601 accgagcgga tgcccggggt ccggtcggtc acgctgggcg tctgggtgcc cgtgggctcc
      661 cgagacgagc ctgcccgcac cgccggcgcc tcgcacttcc tggagcatct tctcttcaag
      721 ggcaccaagg cgcgctcggc tcaggagatc gccgaggcct tcgacgcggt ggggggagac
      781 ctcaacgcct tcacggggcg ggagcagacg gtcttctacg cgagggtcct ggacagggac
      841 ctcccgatgg cggtcgacca cctcacggac atgatcgagc gctcgctcct gcgctcgcga
      901 gatgtcgagg ccgagcggca ggtgatcctc gaggagatcc acatgaccga ggacgccccc
      961 gatcagctcg tccacgacga cttcgcccgg atcttgtggg gcggccaccc gttggggcgt
     1021 ccgatcctgg gcacccggga gacgatcgag gcgatggggc gcgaccagat ccgccggttc
     1081 tggaaacgac actacgtccc cgccaacctc gtgttcgtcg cggcggggaa cgtggagcat
     1141 gaggcgctga ccgggctcat cgagtcgctc gtggggacgg gcgcacccgt ctcgggtcgg
     1201 cgctcgaccg tccgcgacgg agggacccct ccccggccct ccggcatctg ggacgttcgc
     1261 cggcgcaaga ccgagcaggc gcacatcgcc tacggcacgg cgggcctggc ccggcgcgac
     1321 ccccggcgct tcgccttcgg cgtcgtgaac aacgcgctgg gagaggggat gtcgtcccga
     1381 ctgttccagg aggtgcgcga gaagcgcggc ctggcctact ccgtcttctc ctaccacgcg
     1441 atgtacgcgg agaccgggct cttctgcgtc tatgcgggga cgaccccggt ccgggccacg
     1501 gaggtcctgc gggtgatccg gggcgagatc gaggatatcg ccgagaaggg gctggggccg
     1561 gacgagctgg agcgggccaa gggccacatg aagggcggcc tcgtcctctc ccaggaggac
     1621 acttcctcgc ggatgaaccg cctggggaag tccgaggtgg cccacggcga gatcctcacc
     1681 gtggacgagg tgatcgagcg gatcgacgcg gtgacgggcg aggacgtcag ggacgtggcc
     1741 gccgacgtgc tcgggcgcgc gccttcgctg gcggtgatcg ggccgctggg caaggatgcc
     1801 cttcgggagg tgctgacgtg atccgtgtgg gcgtgatcgg ggcctgcgga cgcatgggga
     1861 cgatggccag ccgggcggtg atcgacgatc cggagctctc gctcgtcgcg gccgtccacc
     1921 cgtcgcgggc cggcgaggat ctcgccgtgg cggtggggca cccccgggcg gaagggaggg
     1981 tctccgcgca gctcgaggcc ctcgtgcagg ccgacgccga ggtcgccgtg gacttcacca
     2041 ggcccgacgt cgtgatggac gacgtccgct tcggatgcac ccacgggatc cacatggtcg
     2101 tggggacgac aggagtgggc cccgacgagc aggcctcgat ccgagagtgg ctgaaggact
     2161 cggatgcgaa cgtcgtcgta gcgccgaact tctcgaccgg cgccgtggtc gctcagcggc
     2221 tggccgagga ggcggcccgc cacttcccgg ccgcggagat catcgagctc caccacgagg
     2281 ggaaggtcga cgcgccgtcg gggaccgcgg cggccactgc ccgccggatc gccgccgcgc
     2341 gcggtgaggc ctggaaaggc ccgggggagg agtcggtgga gggcgttcgg ggcggtgctg
     2401 tggaggggat cccgatccac agcgtgcgac tcccgggcct cgtcgcccac caggaggtcc
     2461 tcttcggggg acaggggcag actctctcca tccgtcacga ctcgacggat cgatcctcgt
     2521 tcatgcccgg cgtggtgcta gcggtcaagg aggtcgcccg ccgacccggc ctgacggtcg
     2581 gcctcgaatc cctgctggag ctctagcccg agcggtggcc ggggggatcg cgctcaggtc
     2641 gggagccgga ggtccccctc cacggtgacc cgctcgccgt cgtggccgag gaggacgccc
     2701 tcgcacttcc cgagcgggtc cagcacggtc ccggacagtg cgtagacggc ggcctccagg
     2761 aacgggctgt gccccacgac gagggctcgc tggccgtccc ggatcgcggc gaacatctgg
     2821 aggatcgcgt cggccagccg gggcagctcc tcgcgcacca gctcgggatc ttcccgctcg
     2881 atgtcgtcca cgcgccaggt cccggtgcgg cttccggcct cccgccagcg ctcctcctca
     2941 gcggatgtga gcccgtcggt gacgccgtgg agctggggga gctgctggcc catgccgcgc
     3001 aggaaccagg ccgcggtgtt ggcggcgcgc ttcgcgggcg aggtgaagac gccgtcgaag
     3061 gggccttcca tcgaccggcc cacacgctcg gccgtggcct tccccgcttc cgtgagggcg
     3121 tcgccctcgt tgtcggcgtg ccgacgcagc tcgaggtacc gcactacttg accgtgtgct
     3181 cctcgacgaa gaggagaccg aaggcggagg tgaagctggc caggtcgtcg gtgctctcct
     3241 tggccacggg gctcgccatg tcggccttga agccgtcgcg gtcggcccac tccaggatgg
     3301 ccacgccgtc gtacggcttc tcctgcccct cggggaccct ggtgacgagg gccaccgtat
     3361 agcccgtcag gtgctcgtag ccgtcgccgg cgatcggccc gtgcgtcgtc agccagtagt
     3421 ccttgaagtc ctcatcggac atgccgtcct tcttgcggag ccagatgtgc gccttcacga
     3481 gggcctcctt cgggatgcgg tcaccgaatc ctatccggcg ggaggaggtg gtgggcgccc
     3541 gcctaccgct cgcagccgcg cagcctcccg gtgcccggcc cctggacgca gcgatcccgt
     3601 cccggaccgc cgaacagcgc gtcgttcccg gcctgcccct tgagcacgtc gttgccaccg
     3661 tgcccgcgga gcacgtccgt cccggccccg ccgaacgccc tgtcccggcc cccgccgagg
     3721 cacatggcgt cgtcgccgcc ccgtcctcgg gcgacgtcgt tgcccgagag cgcgaggaac
     3781 ccatcggggc cccgcgtccc ccgaaggacg tcgcgcccct tcgtgccgac ccggttgacc
     3841 acgacacccg cgcaggccgc gagctcgtag gctcccgcgt cgcagcggcc cctgcgcggg
     3901 acgccgcgct ggtccgtcgg ggcgcagtcg gaggcggaca cgagaccgcg cgccctgctc
     3961 ttctgggcca gcgcgtgggt cggcgtgggg ccgccgaggt tgcgcagctt cccgagcttc
     4021 gggttggcga gaacgtcccc cgacaggggg aggaggttgc actcgttggg gcctcgctcg
     4081 atgttgcccc cgagcgtctg gatctggacc cctccggagc agtcggacgt ggccgcgccg
     4141 ctgcccacct tgttgcggcc gaacgcggag gctcgcagct ccacggtccc gatggagacg
     4201 gcgaggccgc ccccgtcacc caccgcgttg ccgtcgaagt cggcccggtt gtccgccacc
     4261 gtgaggtgcg agaaggagga cgacccgctg aagtggtaga tgccgccccc gaagcgcgcc
     4321 gctcgattct tgctcaccgt cacgttctcg aacgtcgcgg tggccccgtt gttgttgttg
     4381 aacccgccgc cgtccccgtc gaaggcgacg ttgccgacga gcgcgctgtc ccggaccgtg
     4441 agcgtcgccg gcgtcgggaa gctcatgatg ccgcccccgt cgcggctccg gttccccttg
     4501 atcagggtgt cgaacaccga cgcggttcca cgattccgta ggccccctcc ggtgccggag
     4561 ccggtggtcc gattgcccac gatgcggctc tcgacgacgg agaggatccc cgtgtcgtag
     4621 ttccagatcc caccgcctcc tcccgtcacc gcgtccagcg actcgttgcc gcgtacctcc
     4681 acgttcttca gcacgaggat gccgttgttc cagatccctc cgcccgaggt gccggtgacc
     4741 gagccgcccg tgatcgtgag gtcgcgaagg acgacgagga tcccggcgaa ccctgtatcc
     4801 acgtcgaaga cgcgatccag gccgttcgcg tcgatgatgg tccttcccat ccccgccccg
     4861 cggatcacga cgtcgtcggt gatgtccagg tcgcccgtga gcccggcgtt gtcgccggcg
     4921 gggcccaccg acagcctgaa gcgcccggcc gggacgcgga tcgtcgtggg gtcgcttcct
     4981 ccgtcgcagt agtcgatcgg gaggtccacg ttcgacgaca cgatcgcc
//
LOCUS       DASUTZ010000220        10622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_724658, whole genome shotgun sequence.
ACCESSION   DASUTZ010000220 DASUTZ010000000
VERSION     DASUTZ010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10622
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724658"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VF097_09585"
     CDS             complement(<1..240)
                     /locus_tag="VF097_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263085.1"
                     /translation="MSTAIPAPDAPPRRRRRPTGQPPPLPKELGRSGRLWVAMFIALA
                     LFLIFLLFLPIGASMERAENAFLRRLVTLRSDPLDS"
     gene            complement(284..412)
                     /locus_tag="VF097_09590"
     CDS             complement(284..412)
                     /locus_tag="VF097_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263086.1"
                     /translation="MKQDLLDLEHRLKGYVLRTILLTNLALAGSFAAIALTAAGLT"
     gene            629..2557
                     /locus_tag="VF097_09595"
     CDS             629..2557
                     /locus_tag="VF097_09595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX6263087.1"
                     /translation="MRTEQAWIPMPDGVRLSATLYLPDDEGLHPVLLEYLPYRKDDGL
                     LSRDHDLYAYMTRAGYVGARVDIRGTGRSEGVVPPFEYSEQEQDDGEAVIEWLASQPW
                     STGAIGMWGISWGGFNAIQMGTRPPPALRAIVALHASDDLFHDDVHYVDGLMHLDEYE
                     LMIDALNPLPPAPDFPTDEETLAARFDREPWILEKMRHQRDGPFWRRGSLRPDYERLE
                     VPALLIGGWYDGYRDTVPRVLEGSRVPTRAIVGPWDHSFPHRASHGAIEWRAEAVRWW
                     DRWLREERSGIEDEPRLAVFVRDHHSPGEVESIPGRWRLERTWPPERLEERTLWPGDG
                     GGLVDSRSAGEHHLPYDPSIGIEAGSWWGEVQPDQSPLDARCLAYETEPLEEPLEILG
                     MPRAELRSSVDAPIANWFVRLSDVAPDGRATLVSGGGLSGAQRVSAEDPSPLEPGEPH
                     DLVVPMRFTSWRFPAGHHIRLAVSNALFPMTWPTPHPMTMTLHLEGSQLVLPVAPPAE
                     LEEPTWEEAGPPEPPEGVRVEGSILPTGWTTRREGSRFVAEAEGEEKMTFPWGREVFR
                     ERLRHEVDVGRPAHAEALGVAQTLIELEGRSLRLDVQISITSDPERFRTQVHRRLTEN
                     DRLVRERSWSEDAPRDHH"
     gene            complement(2561..2890)
                     /locus_tag="VF097_09600"
     CDS             complement(2561..2890)
                     /locus_tag="VF097_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263088.1"
                     /translation="MNARRRLIIGLVAALAVIGVSTAVVVAASGDDQPLPDDARARAT
                     AAALDHVGGGRVLETEIGDDGMAYGVEILKPDGSVVEVSLDEDFQVARVEADDDGARD
                     AEGPDDD"
     gene            complement(2960..4222)
                     /locus_tag="VF097_09605"
     CDS             complement(2960..4222)
                     /locus_tag="VF097_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011598957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6263089.1"
                     /translation="MALVAAAVALVMLLRAALTEDVRLAARLRAEAVADLIAATEPSS
                     EISLGDTDEEFVQVLAPGGEVVSSTENVADRPSVVALEPGESRRVEVPFEDDPFLAVA
                     RGDGGLLIVVGRSLDGVIEVTRTVVGLLAIGFPLLLLVVALVTRRVVGRALAPVEAIR
                     SEVDAISSSELHRRVPVPRAGDEISRLATTMNRMLNRLQRGQDRQRRFVSDASHELRS
                     PVASIRQHAELALTHPAASSPKDLARTVLDEDLRLQQLVENLLLLARMDERGEARLRP
                     VDLDEIASDEVGRLRRVDGRSIDTDGLSGGRMVGDPRQLGPLVRNLLDNAVRHARGHV
                     ALALFEDGDQVVLRVDDDGPGIAQESRERVFERFVRLEDARDRDSGGSGLGLSIVAEV
                     ARAHGGSVELVDSPLGGARFEVRLPRAD"
     gene            complement(4278..4952)
                     /locus_tag="VF097_09610"
     CDS             complement(4278..4952)
                     /locus_tag="VF097_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6263090.1"
                     /translation="MRVLIVEDERGLAEGLRKGLVAEGFAADVALRGDDGLWMARENP
                     YDAIVLDLMLPGMNGYQVCSALREAGVWTPILVLTAMDDELDEAEALDLGADDFLVKP
                     FSYVVLVARLRALIRRGGPERPTVLEAGDLRLDPGARRAWRGDQEVALTARELSLLEL
                     LLRRKGQVVSKREILEHVWDDRFEGNPNIVEVYVRHLRNKLDRPFGRDSIRTVRGAGY
                     RVVADG"
     gene            5212..5832
                     /locus_tag="VF097_09615"
     CDS             5212..5832
                     /locus_tag="VF097_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263091.1"
                     /translation="MTIDPADFVETIDNPYFPLEPGTVLRLEGETDEGMEVDRITVLD
                     RTREVMGVTTTVVLDVVTANGELAEKTWDWFAQDREGNVWYFGEDTAEYEDGEVVSRS
                     GAWEAGVDGALPGIVMPAEPRVSDAHRQEYYRGEAEDMFWVVETGGSKEVPFGSFDDV
                     IHTLEWSPLEPGIVVEKYYAPGVGLIAERALSGGKEIFELVELTRP"
     gene            complement(5845..6489)
                     /locus_tag="VF097_09620"
     CDS             complement(5845..6489)
                     /locus_tag="VF097_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263092.1"
                     /translation="MGRKARALAIGAGAAWGTTYWLGRTWGSTDEERRRHLPGDEVVS
                     PARLVTNHAATIPATPEEIWPWLLQVGWHRGGWYTYRWVDRLLFPANRPSADSILPEY
                     QDLKVGDRIPDGPPESDCYFEVELLEPHKYMVLHSRTHLFQQLREMPGTEMDWTWTFH
                     LEPLGERETRFLFRVRANLDPAWLRALYHVAVVPADFLMSRSMCLGLKKRVERG"
     gene            complement(6693..6887)
                     /locus_tag="VF097_09625"
     CDS             complement(6693..6887)
                     /locus_tag="VF097_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263093.1"
                     /translation="MIGGALFAILPGVEFAPYAAAEMGADVAQAQEILTPYFLPVLLN
                     GGVFFAIGTMASRGPSAPAE"
     gene            complement(7084..8112)
                     /locus_tag="VF097_09630"
     CDS             complement(7084..8112)
                     /locus_tag="VF097_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006127978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6263094.1"
                     /translation="MSDSPLTAPQTPGTTEAPLLEVRDLNVHFRGRVGLGAGLLGRKG
                     SQARAVDGVDLSIRRGEVLVLAGESGCGKTTLARTIMGLEKPTSGQILFEGKPVRPRD
                     VRAYRRQVQMVFQDPTGSLNPRQTIYETVAEGLRIHGIHDEEERRVADALSAAGLRPP
                     EALFLRYPHELSGGQRQRVVIAGALALEPSVIVADEPVSMLDASVKGEILKLLLGLRE
                     RLGLTVLLVTHDLGVAWTVADRVAVMYLGKIVELGPAERVLSEPAHPYTTALLEVAPE
                     HGGPRMLLTGEPPDPTRIPEGCRFHPRCPVVASGRAAAARIEERCRGEIPLLREVGRE
                     HFAACHYA"
     gene            complement(8240..9205)
                     /locus_tag="VF097_09635"
     CDS             complement(8240..9205)
                     /locus_tag="VF097_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019992269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6263095.1"
                     /translation="MSLLSVRDLHVTYSTEGGPVPAVRGVDLELSRGEILGLAGESGC
                     GKSTVAHALLRLLPKGTEVSGRVLLDGDDILAMKPGRLRAVRWAEASIVFQGAMHSMN
                     PVQRVGDQIAEAMVLHGMSPQNGVRKEVASLLERVGLPARRARDYPHELSGGQRQRVM
                     IAMALACSPQILIADEPTTALDVMVQAQVLDLLRELQRDLGLALLFITHDLSVLVDLC
                     DRIAVMYAGRIAEEGPARAVFEDAKHPYAAALSGAFPRIGDPAYRRSPGGLGGDPPDP
                     SALPSGCPFHPRCPERFEPCDGVEPGMFPVGDGRVAACYLLEPGR"
     gene            complement(9202..10143)
                     /locus_tag="VF097_09640"
     CDS             complement(9202..10143)
                     /locus_tag="VF097_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003973312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6263096.1"
                     /translation="MTDSTVAPPAEPGAPPAPPSPRAIAWSRRRRSAARVWRTFRKNR
                     MGMTGLVILVFFVLVALFAPLLADPAGLRATEATGGVLEAPSLDYPLGTDILGRSVLT
                     LTIHGSRISLLVGFAATLLSMLIGSVLGIAAGFYGRWRDAVLMRLTDWFLVIPFLPLA
                     IVLASLIGPSLLTIIFVIGVTSWPGTARLVRAQTLSVKERPYVERARALGAGGWHLVT
                     RHILPNVFPIIFANTTLTVAIAILSETTLSFLGLGDPLRVSWGSILEFAFSAGASTAG
                     AWWWLLPPGLAIVLVVLAFTMVGYALDEILNPRLRSR"
     gene            complement(10140..>10622)
                     /locus_tag="VF097_09645"
     CDS             complement(10140..>10622)
                     /locus_tag="VF097_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6263097.1"
                     /translation="WFPTGGIRTPGAELTGVAAATDVLNHLFLPAITLTLALIGEYAL
                     IMRSSLLDVMGEDYLLTARAKGLREALVLRRHAVRNALLPTVTLVALNLGFVVSGAIT
                     VELVFSWPGLGLLTAEAADVPDFPLLQGLFLLFSVTVILANLVADLIYGYLDPRVRAA
                     "
BASE COUNT         1612 a         3846 c         3731 g         1433 t
ORIGIN      
        1 ggagtcgagc gggtcggatc tcagcgtgac gagccggcgc aggaaggcgt tctccgcgcg
       61 ctccatcgat gccccgatgg ggaggaagag gaggaagatc aggaagaggg ccagcgcgat
      121 gaacatcgcg acccagagcc ggccgctcct gcccagctcc ttggggaggg gcgggggctg
      181 tcctgtcgga cgacgccgcc gccgaggcgg ggcgtcgggg gcggggatcg cggtgctcat
      241 gctgcggcca ttgtgtgccc tttcccgtag cggggccagg gctctacgtg aggcctgccg
      301 cggtgagcgc gatcgcagcg aacgatccag ccagagcgag gttcgtgagc aggatcgtcc
      361 tcaacacgta tcccttgagc cggtgctcca gatcgaggag gtcctgcttc atggcgaggt
      421 tcgggtagcc atccggcggg ggctcgtgag ggggaggcgc ctcggagcct accgagcgtg
      481 tctgacaggg aagggtgcca tgccgggccc tcaggaagac atggcgcgga tccgccatcc
      541 ttctccggtg ggtggagcgc ccgccgccgg cgcccacggc gcggacgcgc gtcacgccga
      601 cagcgccctc ccggcgtaga ctcccggcgt gcgaaccgag caggcctgga tcccgatgcc
      661 cgacggggtc cggctgtccg ccaccctcta cctcccggat gacgaaggcc tgcacccggt
      721 cctcctcgag tacctgccct accgcaagga cgacgggctg ctgtcgcgcg accacgacct
      781 ctacgcgtac atgacccgcg cggggtacgt cggggcgcgg gtcgacatcc gcggcacggg
      841 tcggagcgag ggcgtggtgc cgccgttcga gtactcggag caggagcagg acgacggaga
      901 ggcggtcatc gagtggcttg cctcccagcc ctggtccacc ggcgccatcg ggatgtgggg
      961 catctcgtgg ggaggcttca acgcgatcca gatgggcacg cgtccgccgc cggccctcag
     1021 ggcgatcgtc gccctccacg ccagcgacga cctcttccac gacgacgtcc actacgtgga
     1081 cgggctgatg cacctcgacg agtacgagct gatgatcgac gcgctcaacc cgctcccgcc
     1141 cgcgcccgac ttcccgacgg acgaagagac gctcgcagcg cggttcgatc gcgagccgtg
     1201 gatcctcgag aagatgcggc accagcgcga cgggccgttc tggcgacgcg gctcgctgcg
     1261 gccggactac gagcgcttgg aagtccccgc gctcctgatc ggcggctggt acgacgggta
     1321 ccgggacacc gtgcctcggg tgctcgaggg gtcccgcgtc ccgacccgcg cgatcgtggg
     1381 gccgtgggac cactcgttcc cgcaccgggc ctcgcacggg gcgatcgagt ggcgggccga
     1441 ggccgtccgc tggtgggacc ggtggctccg ggaggagcgg agcgggatcg aggacgagcc
     1501 gcggctcgcg gtcttcgtgc gggaccacca ctcgccgggc gaggtcgagt cgatccccgg
     1561 ccgctggcgc ctcgagcgca cctggcctcc ggaacgtctc gaggagcgca cgctgtggcc
     1621 gggcgacggc ggcgggctcg tcgacagccg atcggccggt gagcaccacc tcccctacga
     1681 cccttcgatc gggatcgagg ccggctcgtg gtggggcgag gtccagcccg accagtcgcc
     1741 gctcgacgcg cgctgccttg cctacgagac ggagcccctg gaggagccgc tcgagatcct
     1801 ggggatgccg cgggccgagc tccggtcgtc ggtggacgca ccgatcgcga actggttcgt
     1861 gcgcctgtcc gacgtggcgc ccgacggccg cgccaccctc gtcagcggag gagggctgag
     1921 cggcgcgcag cgggtctcag cggaggaccc gtcgccgctc gagcccggcg agccccacga
     1981 cctcgtcgtc ccgatgcgct tcacgtcctg gaggttccct gcagggcacc acatccggct
     2041 cgcggtctcg aacgcgctgt tcccgatgac ctggcccacc ccacacccga tgacgatgac
     2101 cctgcacctc gagggatcgc agctcgtgct cccggtggcg ccaccggcgg agctggagga
     2161 gccgacctgg gaggaggcgg gccctcccga gcctccggag ggcgtccgcg tcgagggcag
     2221 catcctgccg acgggatgga cgacgcgccg cgaggggagc cggttcgtcg cggaggccga
     2281 gggcgaggag aagatgacct tcccttgggg gagggaggtg ttccgcgagc gcctgcgcca
     2341 cgaggtggac gtggggcggc ctgctcatgc ggaagcgctc ggggtcgctc agacgctcat
     2401 cgagctggaa ggtcggtccc tccgcctcga cgtgcagatc tcgatcacca gcgaccccga
     2461 gcgcttccgc acccaggtcc accgccgcct caccgagaac gaccgcctgg tccgcgagcg
     2521 ttcctggtcc gaggacgccc cccgcgacca ccactgaggg tcagtcgtcg tcgggtccct
     2581 ccgcgtccct tgctccgtcg tcatcggcct ccacgcgcgc gacctggaag tcctcgtcca
     2641 ggctgacctc gactaccgag ccgtcgggct tcaggatctc cacgccgtag gccatcccgt
     2701 cgtcgccgat ctccgtctcc agcactcggc cccctccgac gtggtcgagc gctgccgccg
     2761 tggccctggc ccgtgcgtcg tcgggaaggg gctggtcgtc gccgctcgcg gcgaccacca
     2821 ccgcggtcga gacgccgatg accgccagcg ccgcgacgag gccgatgatg agccgtctcc
     2881 tcgcgttcat gtcgtcacct ccgagcccat cttgcccggt ccgacctgag ccctcgctga
     2941 actgagcctg aaagtccctt cagtcagcgc gggggagcct cacctcgaag cgagcgccgc
     3001 ccagcggcga gtcgacgagc tccaccgatc ctccgtgggc gcgcgccacc tccgccacga
     3061 tcgagagccc caggccgctc cctcctgagt ctcggtcccg ggcgtcctcg aggcgcacga
     3121 accgctcgaa gaccctctct cgcgactctt gcgcgatgcc cggtccgtcg tcgtccaccc
     3181 gcagaacgac ctggtcgccg tcctcgaaca gggcgagggc cacgtgaccc cgcgcgtgcc
     3241 ggaccgcgtt gtcgaggagg ttgcgcacca ggggccccag ctgacgcggg tcccccacca
     3301 tccgcccgcc gctgaggcca tccgtgtcga tgctccgccc gtcgacccgg cggagccgcc
     3361 cgacctcatc cgacgcgatc tcgtcgaggt ccaccggccg caggcgcgcc tcgccccgct
     3421 cgtccatccg cgccaggagg aggaggttct ccaccagctg ctggagccgg aggtcctcgt
     3481 cgagcacggt cctggcgaga tccttcgggg acgaagcggc cggatgggtc agcgccagct
     3541 cggcgtgctg ccggatgctg gccaccgggg agcgcagctc gtgagaagca tcggatacga
     3601 agcgccgttg acggtcctgg ccccgctgca gccggttcag catccggttc atcgtcgtcg
     3661 ccagccggga gatctcgtcg cccgcgcgcg gcacgggcac ccgacgatgc agctccgacg
     3721 aggagatcgc gtccacctcg gatcggatcg cctccacggg agcgagggcc cggcccacga
     3781 cccggcgggt gacgagcgcc accaccagga ggaggagcgg gaagccgatg gccagcagcc
     3841 ccaccacggt ccgcgtgacc tcgatcaccc cgtcgaggga gcgtcccacc acgatcagga
     3901 gcccgccgtc gcccctcgcc accgcaagga aggggtcgtc ctcgaatgga acctccaccc
     3961 gcctgctctc cccgggctcg agggccacga ccgagggccg atcagcgacg ttctcggtcg
     4021 aggacaccac ctccccgccc ggggcgagca cctggacgaa ctcctcgtcg gtgtcgccca
     4081 ggctgatttc cgaggagggc tcggtagcag cgatgaggtc ggccaccgct tccgcccgga
     4141 gcctcgccgc cagacgcacg tcctcggtca gcgccgcccg gagcaacatc accagcgcca
     4201 cggcggccgc caccagggcc acgcccacca cgagcgtcgc acctaccgtg gtccgagtcc
     4261 ggacggagcc tcccctctca cccatcggcc accacccgat atccggcgcc ccggaccgtc
     4321 ctgatcgagt cccggccgaa cggccggtcg agcttgttcc gcaggtgacg gacgtagacc
     4381 tcgacgatgt tggggtttcc ctcgaacctg tcgtcccaga cgtgctcgag gatctcgcgc
     4441 ttcgacacga cctgcccctt cctccggagg aggagctcga gcagggacag ctcccgagcc
     4501 gtgagcgcga cctcctggtc tccccgccac gcgcgacgag ctcccgggtc cagccggagg
     4561 tcgcccgcct cgaggaccgt gggacgctcg ggaccgccgc gccggatcag ggcccggagc
     4621 cgggccacca gcacgacgta cgagaagggc ttcacgagga agtcgtccgc tcccagatcg
     4681 agggcctcgg cctcgtccag ctcgtcgtcc atcgcggtga ggacgaggat cggcgtccac
     4741 acgcccgctt cccggagcgc cgagcacacc tgatacccgt tcatgccggg cagcatgagg
     4801 tcgaggacga tggcgtcgta ggggttctcg cgggccatcc agaggccgtc gtcgcctctc
     4861 agcgccacat ccgcggcgaa cccctcggcc acgaggccct tcctcagccc ctcggcgagc
     4921 cctcgctcat cctccacgat cagcacgcgc atgccgtcct ccgtcgggcc cggccctcca
     4981 ccatgatgga cccatcctga agctcggctg aatggagtga gcggccattc agctgcgcgt
     5041 cagcgagccg ggcgcatgct cccaccgttc cggacgagag gagcggagat gagcatcccg
     5101 gccagacgtg cggcgttggt gttcacggtc ctcgcgatgg ccctactcgg ggccgggtgt
     5161 ggcggggaac cctctccatc ggtcatcgat gaggcaccag gagggtacgc ggtgacgatc
     5221 gacccggccg acttcgtgga gacgatcgac aacccctact tcccgctcga gcccggcacc
     5281 gtcctgcgcc tcgagggcga gaccgacgag gggatggagg tcgacaggat cacggtcctg
     5341 gaccggaccc gggaggtcat gggcgtgacg accaccgtcg tcctggacgt cgtcacggcg
     5401 aacggggagc ttgcggagaa gacgtgggac tggttcgccc aggaccgcga gggcaatgtg
     5461 tggtacttcg gcgaggacac ggccgagtac gaggacggcg aggtcgtgag ccggtcggga
     5521 gcctgggagg cgggggtgga cggggcgctt ccggggatcg tcatgccggc cgagcccagg
     5581 gtctcggacg cccatcggca ggagtactac cggggcgagg ccgaggacat gttctgggtg
     5641 gtcgagaccg gcgggtcgaa ggaggtgccc ttcggctcgt tcgacgacgt gatccacacg
     5701 ctcgaatgga gcccgctgga acccgggatc gtcgtggaga agtactacgc gccgggcgtg
     5761 gggctcatcg cggagcgcgc gctctcgggc ggcaaggaga tcttcgaact cgtggagctc
     5821 acgcgcccgt gacgcggatg gggtctagcc gcgctccacg cgcttcttga ggccgaggca
     5881 catgctgcgg gacatcagga agtcggccgg gacgactgcc acgtggtaga gggcccgcag
     5941 ccacgcaggg tcgaggttcg ctcgcacgcg gaagaggaac cgcgtctccc gctcgcccaa
     6001 cggttcgagg tggaaggtcc acgtccagtc catctccgtg cccggcatct cccggagctg
     6061 ctggaagagg tgggtcctgg agtgcagcac catgtacttg tgcggctcga gcagctcgac
     6121 ctcgaagtag cagtcggact cgggcgggcc gtcggggatc cggtcgccga ccttcaggtc
     6181 ctggtactcg ggaaggatcg agtcggcgct cggccggttt gcggggaaga ggagacggtc
     6241 cacccaccgg tacgtgtacc agccgccccg gtgccagccg acctggagga gccacggcca
     6301 gatctcctcg ggtgtcgcgg ggatggtcgc ggcgtgattg gtgacgagcc gggccgggct
     6361 gacgacctca tcgccgggga gatgccgccg gcgttcctcg tccgtcgacc cccacgtccg
     6421 accgagccag tacgtggttc cccacgctgc tcccgcaccg atcgcgagag ctctggcctt
     6481 ccgtcccatc gcgctacctc cttggcgaga gtgtgacggt aggcatcgga accgcggagg
     6541 ggccactcgt cccccagaag aggggacgag tctcccatga ggggactggc tcctctaacc
     6601 ggtggccggc tcgccgacca tctgggcctc gggtcggccc cacatcgtcg ccgcgatcgg
     6661 caacagggcc acgagggccg tccccctgct catcactccg ctggcgccga tggcccgcgt
     6721 gaagccatag tcccgatggc gaagaacact ccaccgttca gcaggacggg gaggaagtag
     6781 ggggtcagga tctcctgggc ctgcgcgacg tcggccccca tctcggccgc ggcgtagggt
     6841 gcgaactcga cgccgggcag gatcgcgaag agggcgcccc cgatcacggc gagcggcagc
     6901 gcccggccgc cgccacgagc ggcgcgacga ccacgagcgc cgccccccat cgtgagctgt
     6961 tctcggtctg cgtcatctgc ctcgcctcct cggggggcat cgggccgccc gcttctgccc
     7021 cgaccgtagg cacgcggagc ctgggcagga atcggaagtt ctcctccatg tggacgggcc
     7081 cggctacgcg tagtggcagg cggcgaaatg ctctctcccg acttcgcgca ggagggggat
     7141 ctcgccgcgg cacctttctt ctatccttgc ggccgctgcc cgacccgacg cgacgaccgg
     7201 gcaccttgga tggaaccgac acccttcggg gatccgcgtg ggatccgggg gctcgcccgt
     7261 caggagcatc cggggcccgc cgtgctccgg ggccacctcg agcagggccg tcgtgtacgg
     7321 gtgcgcgggc tcgctgagca cccgctccgc cgggccgagc tccacgatct tcccgaggta
     7381 catcaccgcg acacggtcgg ccaccgtcca cgccacgccc aggtcgtgcg tgacgagcag
     7441 gaccgtcagc ccgaggcgct cgcggaggcc gagcagcagc ttcaggatct cccccttgac
     7501 cgaggcgtcg agcatcgaga ccggctcgtc cgcgacgatg acgctgggct cgagcgcgag
     7561 cgcgcccgcg atcacgacgc gctggcgctg gcccccgctc agctcgtggg ggtaccggag
     7621 gaacagcgcc tccggcggac gcagccccgc ggccgacagc gcgtccgcca cgcggcgctc
     7681 ctcctcatca tggatcccgt ggatgcggag gccctcggcc acagtctcgt agatcgtctg
     7741 gcgggggttg agcgacccgg tcgggtcctg gaagaccatc tgcacctgcc ggcggtaggc
     7801 gcggacgtcg cgcgggcgga cgggcttccc ctcgaagagg atctgccccg aggtgggctt
     7861 ctcgaggccc atgatcgtcc gggccagggt ggtcttgccg cagccggact cgccggccag
     7921 gacgaggacc tcgccccgcc ggatggacag atccacgccg tccaccgctc gcgcctggga
     7981 gcccttccgg ccgagcaggc ccgcgccgag ccccacccgc ccgcggaagt ggacgttgag
     8041 gtcgcggacc tcgaggagag gggcctcggt cgtgccgggg gtctgtggcg cggtgagcgg
     8101 cgagtcgctc atggtgctcg gctcccaggg gtggtccgga tagcggacag gtgaccggga
     8161 tggcggacac ccgtacgggg cacccccctg agccccgctc catggcggtg aacgccggcg
     8221 ggcaggaggc ctcgcccgct catcggcccg gctccagcag gtagcaggcc gccacgcgcc
     8281 cgtcgcctac agggaacatc cccggctcca cgccgtcgca gggctcgaag cgctccgggc
     8341 accgcgggtg gaacgggcac ccgctcggga gcgcggacgg gtcgggaggg tcgccgccca
     8401 gcccgcccgg cgatctccgg tacgccgggt cgccaatgcg ggggaaggcg ccggacagcg
     8461 ccgccgcgta ggggtgcttc gcgtcctcga acaccgcgcg cgccggcccc tcctcggcga
     8521 tccggcccgc gtacatcacc gcgatccgat cgcacaggtc cacgagcacc gagaggtcgt
     8581 gcgtgatgaa cagcagcgcc agaccgagat cgcgctggag ctcacgcagc aggtcgagca
     8641 cctgcgcctg aaccatcacg tccagcgctg tggtgggctc gtcggcgatc agaatctggg
     8701 ggctgcacgc cagcgccatc gcgatcatca cccgctggcg ctgcccgccc gagagctcgt
     8761 gcgggtagtc gcgggcccgg cgtgcgggca gtcccacccg ctccagcagc gaggccacct
     8821 ccttccgcac cccgttctgc ggcgacatcc cgtgcaggac catcgcctcc gcgatctggt
     8881 cgcctacgcg ctggaccggg ttcatcgagt gcatcgcccc ctggaagacg atcgaggcct
     8941 cggcccaccg caccgcccgc aggcggcccg gcttcatcgc caggatgtcg tccccgtcca
     9001 ggagcacccg cccgctgacc tcggtccctt tgggcagcag ccgcagcagc gcgtgcgcca
     9061 cggtcgactt cccgcagccc gactcgccgg ccagccccag gatctcgccc cgcgacagct
     9121 ccaggtccac cccccgcacg gccggcaccg gcccgccctc ggtcgagtac gtcacgtgga
     9181 gatcccgaac cgacagcagg ctcatcggga cctcagtctc gggttgagga tctcgtcgag
     9241 cgcgtacccc accatcgtga acgccagcac gacgagcacg atcgccagcc ccggcggcag
     9301 cagccaccac cacgcgccgg ccgtcgaggc ccccgcactg aaggcgaact ccaggatgct
     9361 gccccacgac acccgcaagg gatcgcccag ccccaggaac gacagcgtgg tctcggacag
     9421 gatcgcgatc gccaccgtca gcgtcgtgtt ggcgaagatg atggggaaga cgttgggcag
     9481 gatgtgccgg gtcacgaggt gccatcctcc ggcgcccagc gcccgggctc gctcgacgta
     9541 gggccgctcc ttgacggaca gggtctgcgc ccggaccagc cgagcggtcc ccggccacga
     9601 cgtcaccccg atcacgaaga tgatcgtgag cagcgagggc ccgatcagcg acgccagcac
     9661 gatcgccagc ggcaggaacg ggatcaccag gaaccagtcg gtgagccgca tcagcacggc
     9721 gtcccgccag cgcccgtaga agccggccgc gatcccgagc acggacccga tcagcatcga
     9781 gagcagggtc gccgcgaacc ccaccagcag ggagatgcgc gagccgtgga tcgtcagcgt
     9841 gaggaccgac cgcccgagga tgtcggtgcc gagcgggtag tcgaggctgg gggcctcgag
     9901 gacgccgccg gtggcctcgg tggcgcgcag gccagcgggg tcggccagca ggggagcgaa
     9961 cagcgccacg agcacgaaga agacgaggat caccagcccg gtcatgccca tccggttctt
    10021 ccggaacgtg cgccagaccc ttgcggcgct ccgccggcgc cgggaccagg cgatggcgcg
    10081 cggcgagggg ggcgccggcg gagcgcccgg ctcggccggg ggcgcgacgg tggagtcggt
    10141 cacgccgccc tcaccctcgg gtccaggtag ccgtagatca ggtcggccac caggttcgcc
    10201 aggatcaccg tgacgctgaa gagcaggaac aggccctgga gcagcgggaa gtcgggcacg
    10261 tcggcggctt ccgccgtgag caggccgagc ccgggccacg agaacacgag ctccacagtg
    10321 atggcccccg agaccacgaa ccccaggttc agagcgacca gcgtcacggt ggggagcagc
    10381 gcgttccgga cggcgtggcg ccgcaggacc agcgcctcgc gcagtccctt agcccgagcc
    10441 gtcaggaggt agtcctctcc catcacgtcc agcagcgacg accgcatgat cagcgcgtac
    10501 tcgccgatca gcgccagcgt cagcgtgata gccggcagga acagatggtt gaggacgtcg
    10561 gtggctgcgg cgacccccgt gagctcggcg ccgggcgtcc ggatcccccc ggtcgggaac
    10621 ca
//
LOCUS       DASUTZ010000221        15528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_76858, whole genome shotgun sequence.
ACCESSION   DASUTZ010000221 DASUTZ010000000
VERSION     DASUTZ010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 15528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15528
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76858"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VF097_09650"
     CDS             complement(<1..465)
                     /locus_tag="VF097_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX6263098.1"
                     /translation="MTREALPSLRVPDPTLETRVASRLEEIEAALERAVLSDIRLVAE
                     AGRHLIAAGGKRFRPTLVVLGSHFGDPDVPEITPGAVAIELTHLASLYHDDVIDGADS
                     RRGAPSVNAEWGNTIAVLTGDYLFARSSEISADLGTDVSRLFAATIARVCEGQ"
     gene            complement(490..1929)
                     /locus_tag="VF097_09655"
     CDS             complement(490..1929)
                     /locus_tag="VF097_09655"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX6263099.1"
                     /translation="MAPEIVLRPIVPEMIVAGAAMAILLVDAFRSSRDRLPLALIALA
                     AVVGAAASAISLWGWDGPGTVLGGMVASDRYGVFFRLVILGVAALAILLSLHGRDEER
                     GGEFYALLLFSTTGMSLLAVSSDLIMVFLSLEMLSLSLYVLAGSSIRRPASREAGMKY
                     FLLGAFSTAFLLYGIALAYGATGTTSLSGIAGALAGRTDDLGLALAAAALLTVGFSFK
                     VSAVPFHMWTPDVYQGAPTAVTAFMSAGTKVAGFAALLRVASSALGPVGWDLTPALWL
                     VAALTMVVGSVLAIAQRDVKRLLAYSSVAHAGFVLVGVVAANQQGISGAMFYLVAYAA
                     MILGAFGIVMIVAGRDEERTTMEDYAGLARRSPVLAASLGLFLLSLAGLPPTAGFIAK
                     VLVFQAAVDAGHWALVVVGVVATVIAAYFYIRVIVAAYMEEPEDRVAVSRAPVPGFAV
                     GVAAVLTLLFGVLPFLLLGVLDSASILRF"
     gene            complement(1933..3525)
                     /locus_tag="VF097_09660"
     CDS             complement(1933..3525)
                     /locus_tag="VF097_09660"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004500.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX6263100.1"
                     /translation="MSGWPWLSLTLWAPIAGLVLLLLLTPLSGRRAREEPEDEAEPPP
                     HRGVLVAGPSARVLLWRWGALAVTLATFLISLGVLAFFEGGEPGYQLVERAQWVESPN
                     LQYLLGVDGISLFMVLLTTFLMPISVLASWRQDERVPAFMASLLILETALLGTFLALD
                     LLLFFVFFEGMLFPMYLLIGIWGYEQRVYAAVKFFIYTMAGSALLLAAILFLYFRAGA
                     ELETATFDLAALQGMDLPVVTGRWLFLAFLLAFAVKVPLFPLHTWLPDAHTEAPTAGS
                     VLLAGVLLKIGAYGLIRFNLGLFPEASMYFRELVLVLAAIGIVYGGVVALIQRDVKRL
                     VAYSSVSHLGFVVLGIFAGSVQGMSGGVIQMLNHGLTTGALFLLVGMLYERTHTRDLD
                     RLGGLARQAPVFAGVFLVVALSSLALPGLNGFVGEFLILLGSFFSARPYAVVAAIGLI
                     LSAIYLLWAYQRMMHGEERPELDGFRDLSGREYLVLVPILALIVAIGVYPKPFLERIE
                     PSAEQPARVVQVVEPPVAAEGG"
     gene            complement(3522..5405)
                     /gene="nuoL"
                     /locus_tag="VF097_09665"
     CDS             complement(3522..5405)
                     /gene="nuoL"
                     /locus_tag="VF097_09665"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014137608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX6263101.1"
                     /translation="MTADGPASLIWLALALPLAGAVLLLLLGRPLQRAAGWIASGAVG
                     GAFVAALAAFVDVVGRSGDARSLSVRLFDWISVGSFEAPFELRVDPLSLVMVLTVTGV
                     SFLIHVYSIGYMRDDPRHPRYFAYLNLFVFSMGALVLADNFVVLYLGWEAVGLCSYLL
                     IAFWYERKAAADAGKKAFIVNRVGDFGFALGIFLIFQATGSVRFDEVFGAAGGLPEGT
                     ATAISFLLLAGAVGKSAQLPLHVWLPDAMEGPTPVSALIHAATMVTAGVYLVVRAHVL
                     FEASEIALFAVAIVGTVTAIYAATSALGQDDLKRVLAYSTISQLGFMFLAAGVGAYGV
                     AIFHLVTHAFFKAVLFLGAGSVMHATGGVIDVKRLGGLGRVMPWTTLFFGIGGLALVG
                     LPPLSGFFSKDQVLAAAYGAGHEWLWLVALVTAGITALYATRAFVLTFLGTSRLDEGH
                     PHESPAVMLAPMAVLAVGAAAAGLLGLSAAGGLLHGFMGLGEVAHAEGGLSEGALAVI
                     ATAVAVAGVLIGWLIWGSGRIDWAALRVRYGSLQRAMARGWYVDDAYGNLVVLPGKAA
                     SAFLAYVFDKRVLDGAVNGLGRAFRGLAGAGRRVQTGFVRTYALAFLLGVVGLLALVV
                     LRT"
     gene            complement(5425..5724)
                     /gene="nuoK"
                     /locus_tag="VF097_09670"
     CDS             complement(5425..5724)
                     /gene="nuoK"
                     /locus_tag="VF097_09670"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX6263102.1"
                     /translation="MPIAWYLATSGILFTIGTVGVLVRRNALVIFMAIELQLNAVNLT
                     LIAFARQLGEVDGQVLAFFSMVVAAAEVVVGLAIIVSIFRRRASASVDDLSLLKW"
     gene            complement(5727..6299)
                     /locus_tag="VF097_09675"
     CDS             complement(5727..6299)
                     /locus_tag="VF097_09675"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005165.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX6263103.1"
                     /translation="MGELITFWVTAPISVGAAALMLFQRNAVHAALLLVLNQFTLAVF
                     FVLLDAEFLAAVQVIVYAGAIMVLFLFVIMFLGVDRRESLRERLRGQRPLAIALGLFL
                     VIAILFVVQIGVGLGQRTTEEMPRRAGNVQEVGRVLFTDYVFPFEVTSILLIIAAVAA
                     MVLARRSRSVPEAGEPAASGDDATTAEVPR"
     gene            complement(6346..6762)
                     /gene="nuoI"
                     /locus_tag="VF097_09680"
                     /pseudo
     CDS             complement(6346..6762)
                     /gene="nuoI"
                     /locus_tag="VF097_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018505167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
     gene            complement(6759..7898)
                     /gene="nuoH"
                     /locus_tag="VF097_09685"
     CDS             complement(6759..7898)
                     /gene="nuoH"
                     /locus_tag="VF097_09685"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX6263104.1"
                     /translation="MSAFDAGNWVDWLILLIRVLILFAALMVAVLLTIWAERKLVADM
                     QTRLGPNRAGPFGILVTLADGIKLFFKEGLTPASADRPVYLLAPIVSLIPAFLAFAVI
                     PFGTSISLFGREIPFQLADLNIGILYVLALGSLGVYGVVLAGWSSGTKYPLLGAVRSS
                     AQMVSYEISLGLGLVAVLMYSGTLQMSEIVARQDTIWNVIPQAPAFFIFGISAMAETN
                     RAPFDLPEAESELVAGFHTEYSGIRFAMFFLAEYLHMITVSAVAVTVFFGGPRGPVFD
                     FLPWLWPLLWFLLKLIALIFVFVWIRATLPRFRYDRLMNFGWKVLIPWGLIWVVATGA
                     IVILPDLYGRDTFLRWAAIAAGVALLASVVAPLFSRKPEEEARAG"
     gene            complement(7895..10273)
                     /locus_tag="VF097_09690"
     CDS             complement(7895..10273)
                     /locus_tag="VF097_09690"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005895.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEX6263105.1"
                     /translation="MSVTLTIDGRRVTVPEGTLIIRAAESIGIEVPRFCDHPYLDPVG
                     ACRTCLVEVEGQAKLVTSCTTPVAPDMVVRTQNTSEGAAKGQRGQLEFLLINHPLDCP
                     VCDRGGECPLQDQTLAFGPAESRYIDPKRVYEKPVELSPQVLLDRERCVLCARCTRFC
                     DEISGERFIELFERGAAEQVSISPGEDFASPFSGNTVQICPVGALTNASYRFAARPFD
                     VKRGDSICPHCACGCSIRVDTRRGEVVRHLAKDEPEVNEAWLCDKGRNAFRFPDRERL
                     ETPMLRVPGLEPASHAEVFAEIAARAKGEARAAVLGGARLTDEDAYAMAKLARVAFRT
                     NDLDHRMGVGTGSWDLERVQAAPGTPVTYGDVERAAAILVVGLDTREELPILHLRIRK
                     AHRKGGAKVFVLHPRRTALDVAEHVLCPPGAEAAALAELRSGSTDAANALREAGEGLV
                     VLAGPRLAESPEALEQAASLAADVGGRIAYLTRRANDRGAMRAGVHPALLPGGRRLDD
                     PAERAEVEAVWGGSLPAEPGRSTREILEAAAAREIDLLFLGGVDPLTDFPDQQLVRRA
                     LANAGFTVIVDISVGEYEPYADAVIPAAAFLEKAGHLTDWEGRGQRMAPVRDPVGLSR
                     PEWRIFQELSKVLGTDLGFRSLEDVQAELGDLLAPREVPAPASVPALTKDREVEGLSL
                     FTYPLLVDGGRLSVDADELKAALHREPFLEVHPTDAERLGVRDGGRARIRTERGEAEL
                     PVVVTDGIHEGAVFVPYNQEGLRMSELLTGSTITAVELEAAPEPVEALEGAG"
     gene            complement(10270..11577)
                     /gene="nuoF"
                     /locus_tag="VF097_09695"
     CDS             complement(10270..11577)
                     /gene="nuoF"
                     /locus_tag="VF097_09695"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX6263106.1"
                     /translation="MEGSEKIDSYLEGGGYEALRKALGMSPDDVIEEVKASGLRGRGG
                     AGFPTGVKWSFIPKDTGKPVYLACNADEGEPGTFKDRELMERDPHQLLEGIAIASYAV
                     GCHQAFIYMRGEFLEPARRVERAIAEAYDRGFLGKGIAGSGFDLDVVMHRGAGSYECG
                     EETALLESLEGYRGQPRLRPPFPAVAGLYGSPTVINNVETLSTVPHVIRMGAEEFAKL
                     GTEKSTGTKIFCLSGKVERPGNYEAPMGTPARVIVEELAGGVRGGKALKAWVPGGSST
                     PFLTPDHLDTPMDFESISEQGSLLGTGAVVVLDEDDCIVDAVLRWTQFYAHESCGKCT
                     PCREGTWWMTKVLHRMEHGGGRLDDLDMMVDVGDNILFKSLCALGDGSVSPIDSSIKH
                     FREEYVEHVKVGGCPRKERPKLEAATPRAGGAAGAIPISEVLP"
     gene            complement(11615..12154)
                     /locus_tag="VF097_09700"
     CDS             complement(11615..12154)
                     /locus_tag="VF097_09700"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX6263107.1"
                     /translation="MVFEGPVREQAESIVARYPRSRSALLPLLYLAQSVEGHLSREGM
                     REVGELLGLTTAEVEAVATYYTMYRREPAGRWLLSVCTNVSCALLGARDVHEAARGAA
                     GGDEDVEVEEVECLGACDAAPVVQVNYCNYDRVSEEGVVELIEALRAGEPPPPARGPK
                     PAPHGATSRILAGLGDDRE"
     gene            complement(12154..13392)
                     /locus_tag="VF097_09705"
     CDS             complement(12154..13392)
                     /locus_tag="VF097_09705"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014351470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX6263108.1"
                     /translation="MAQGVQAQRLAEDLMVINMGPQHPSTHGVLRLVLTLDGETVLDC
                     QPVVGYLHTGIEKNIEYRTWTQGSTFVTRADYLSPFSNELAYAHSVERLLGIEAPPRA
                     QAIRVMVCELGRIASHLVWLATTGLELGALSMMLYGFREREKLLDVFEAITGLRMNHA
                     YIRPGGLVMDLPEGAEQRIRALIVDMDGKVDEYESLLSGNPIWVERNRGVGILTAEQC
                     RTWTVTGPLLRAAGVPHDLRTTDPLYEGYDFEIPTREEADCYARYEVRVAEIRESLKI
                     VEQVLDRLPGGRVMVDDPKIAWPAQLEVGPDGIGNSERYVRHIMEESMEALINHFKLV
                     TQGVKVPAGEVYTCVESPRGELGFYVVSDGGVRPYRVRIKDPSFVHVQALPEIVRGGL
                     VADVIASIASVDPVMGGVDR"
     gene            complement(13429..13923)
                     /locus_tag="VF097_09710"
     CDS             complement(13429..13923)
                     /locus_tag="VF097_09710"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX6263109.1"
                     /translation="MSPDELAGRLGARFPDVLVARGEVTITIGRDELVDALRFLRDEP
                     DLGFDFLSDLSCTDWPGREPRIWMAYHLSSGQGMRVRVKAGLPEDDLRIATATEVHPS
                     ANWHEREVYDFFGVTFDGHPDLRRIELPEEWVGHPLRKDEPLGGVATQYKGAYVPPPD
                     QREL"
     gene            complement(13944..14429)
                     /locus_tag="VF097_09715"
                     /pseudo
     CDS             complement(13944..14429)
                     /locus_tag="VF097_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
     gene            complement(14426..14785)
                     /gene="ndhC"
                     /locus_tag="VF097_09720"
     CDS             complement(14426..14785)
                     /gene="ndhC"
                     /locus_tag="VF097_09720"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX6263110.1"
                     /translation="MLVEYLPIVILAGLAAVFAVASLAASALLRPRKPTPAKMAPYEC
                     GIVPEHEPPERFPVKFYVIAMLFIIFDIETIFLFPWAVAFRQLGLFGLVEMVIFIGLL
                     FVAYVYVWKRDGLEWAR"
     gene            complement(14807..>15528)
                     /locus_tag="VF097_09725"
     CDS             complement(14807..>15528)
                     /locus_tag="VF097_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6263111.1"
                     /translation="PIGIAARRYFRSPLANERHFLSWLDLEHQGGFLPGYGWIFPVGD
                     GIVNVGAGLLNTARDFRHLSAKKVYEVFLRRLPPELGITEETAEGRLLSGPLPMGMNR
                     APLAVPGLLVAGDAGGIINPFNGEGIAYAMESGRLAAELIHEALVLDRPGLAQVYPAV
                     LRQRYGKYFSMGRAFVRAIGSPTVMRTAVRYGLPRERLMRFLFKLMANLTDGRDGDVD
                     DRLIHLLTRLAPSRGGFARVL"
BASE COUNT         2587 a         5683 c         5306 g         1952 t
ORIGIN      
        1 ctgcccctca cacaccctcg cgatcgtggc ggcgaacagc cgggagacgt cggtccccag
       61 gtccgccgag atctcggatg acctggcgaa caggtagtcc ccggtgagga cggcgatcgt
      121 gttcccccac tccgcgttca cggagggcgc gccccgccgc gagtcggcgc cgtcgatcac
      181 gtcgtcgtgg tagagcgagg ccaggtgggt gagctcgatc gccacggccc cgggtgtgat
      241 ctccgggacg tcggggtcgc cgaagtgaga tccgagcacg acgagggtgg gccggaaccg
      301 cttccccccc gcggcgatca ggtggcgtcc ggcctcagcc accagccgga tgtcggagag
      361 cacggcccgc tccagcgcgg cctcgatctc ctcgagccgg ctcgccacgc gcgtctccag
      421 cgtggggtcg ggaacccgca gcgacgggag ggcctcacgc gtcatgggag gcgatggtac
      481 cagcgggcat cagaacctca ggatcgacgc gctgtcgagc acgccgagga ggaggaaggg
      541 caggaccccg aacaggagcg tgaggaccgc agccacgccc accgcgaacc ccgggaccgg
      601 agcccgggag accgcgacgc gatcctcggg ctcctccatg tacgcggcca cgatcacgcg
      661 gatgtagaag tacgcggcga tcaccgtggc gaccaccccg accaccacga gcgcccagtg
      721 gccggcgtcc acggcagcct ggaacacgag gaccttggcg atgaacccgg ccgtgggagg
      781 caggccggcc agggagagca ggaacaggcc gagcgacgcc gcgagcaccg ggctgcgacg
      841 ggccaggccc gcgtagtcct ccatcgtggt ccgctcctcg tcgcggccgg ccacgatcat
      901 aacgatcccg aacgccccga ggatcatggc cgcgtaggcc accaggtaga acatcgcccc
      961 cgagatcccc tgctggttgg cggccaccac gcccacgagc acgaacccgg cgtgggccac
     1021 cgaggagtac gccagcagcc gcttcacgtc ccgctgcgcg atggccagca ccgagcccac
     1081 gaccatggtc agggccgcca ccagccagag cgccggggtg aggtcccagc ccaccgggcc
     1141 cagcgccgat gaggccacgc ggagcagcgc cgcgaacccg gccaccttcg tcccggccga
     1201 catgaacgcc gtcaccgcgg tgggtgcccc ctggtacacg tccggggtcc acatgtggaa
     1261 cgggactgcc gacaccttga acgagaaccc caccgtcagg agggcggcgg cggccagagc
     1321 cagcccgaga tcgtcggtcc tcccggccag ggcacccgcg atccccgaga ggctggtcgt
     1381 gccggtggcc ccgtaggcca gggcgatgcc gtagaggagg aacgcggtgg agaaggcccc
     1441 caggaggaag tacttcatcc ccgcctcccg tgaagcgggg cggcggatcg acgagccggc
     1501 cagcacgtag agcgagaggg agagcatctc cagagacagg aacaccatga tcaggtcgga
     1561 cgagacggcg agcaggctca tcccggtggt ggagaagagc agcagcgcgt agaactcgcc
     1621 cccccgctcc tcgtcccgcc cgtggaggga gaggaggatc gccagcgcgg cgacgcccag
     1681 gatcacgagc cggaagaaga cgccgtagcg atcggaggcg accatcccgc ccagcaccgt
     1741 ccccggcccg tcccatcccc agagcgagat cgccgaggcc gccgctccga cgacggcggc
     1801 cagcgcgatc agcgccaggg gcaggcggtc ccgcgaggag cggaacgcgt cgaccagcag
     1861 gatcgccatc gcggccccgg ccacgatcat ctcggggacg atcggtcgca gcacgatctc
     1921 cggcgccacg gctcagcctc cctccgccgc gaccgggggc tccacgacct ggaccacgcg
     1981 cgccggctgc tcggccgagg gctcgatccg ctcgaggaac ggcttcgggt agaccccgat
     2041 cgccacgatc agggccaaga tcgggaccag gacgaggtac tcgcgacccg agaggtcgcg
     2101 gaacccgtcg agctccggac gctcctcgcc gtgcatcatc cgctggtacg cccataggag
     2161 gtagatcgcc gagaggatca gcccgatcgc cgccaccacg gcgtagggcc gggccgagaa
     2221 gaacgagccc agcaggatga ggaactcccc gacgaacccg ttgaggcccg ggagagccag
     2281 cgacgagagc gccaccacga ggaacacgcc ggcgaacacc ggggcctgcc tggccaggcc
     2341 tccgagccgg tcgagatccc tggtgtgggt cctctcgtac agcatcccca ccaggaggaa
     2401 cagcgcgccg gtggtgagcc cgtggttcag catctgaatc accccgcccg acatcccctg
     2461 gacgctcccg gcgaagatcc ccagcaccac gaacccgagg tgcgagacgc tcgagtacgc
     2521 cacgaggcgc ttcacgtcgc gctggatcag ggccaccaca cccccgtaca cgatcccgat
     2581 cgccgcgagc accagcacga gctcgcggaa gtacatcgag gcctcgggga agagcccgag
     2641 gttgaaccgg atcaggccgt aggccccgat cttcagcagg acccccgcca gcagcacgga
     2701 ccccgccgtc ggcgcctcgg tgtgcgcgtc gggcagccag gtgtggagcg ggaacaacgg
     2761 caccttcacc gcgaacgcca gcaggaacgc caggaagagc caccgcccgg tgaccaccgg
     2821 gagatccatc ccctgcagcg ccgccaggtc gaacgtcgcc gtctccagct cggcccccgc
     2881 ccggaagtag aggaagagga tcgccgcgag cagcagggcc gagccggcca tcgtgtagat
     2941 gaagaacttc acggccgcgt agacccgctg ctcgtacccc cagatcccga tcaggaggta
     3001 catcgggaag agcatcccct cgaagaacac gaagaagagg aggaggtcca gggccaggaa
     3061 ggtgccgagc agcgccgtct ccaggatcag cagcgatgcc atgaacgcag ggacccgttc
     3121 gtcctggcgc cacgacgcca gcaccgagat cggcatgagg aaggtggtga gcagaaccat
     3181 gaagaggctg atgccgtcca cgccgaggag gtactggagg ttcggggact ccacccactg
     3241 agcccgctcc acgagctggt acccgggctc cccgccctcg aagaacgcga ggacgccgag
     3301 tgagatcagg aaggtggcca gcgtgacggc gagcgccccc caccgccaga ggaggacccg
     3361 ggccgagggc cccgccacga gcacaccgcg gtggggggga ggctcggcct cgtcctccgg
     3421 ctcctcccgg gcccgccgcc cgctgagcgg ggtcagcagc agcagcagca cgagccccgc
     3481 gatcggggcc cacagggtga gcgagagcca gggccagcca ctcatgtcct gagcaccacg
     3541 agggccagga gccccacgac cccgagcagg aacgccagcg cgtacgtgcg aacgaagccc
     3601 gtctggaccc gccgccccgc ccccgccagg cctcggaagg ccctccccag cccgttgacc
     3661 gcgccgtcca gcacgcgctt gtcgaagacg taggccagga acgccgaggc cgccttcccc
     3721 gggagcacca cgaggttccc gtacgcgtcg tccacgtacc agccgcgggc catggcccgc
     3781 tggagggagc cgtagcgcac ccgcagggcg gcccagtcga tccgaccgga cccccagatc
     3841 agccagccga tcaggacgcc ggcgacggcc accgccgtgg cgatcacggc cagagctccc
     3901 tccgagagcc cgccctcggc gtgggccacc tcccccagcc ccatgaaccc atggaggagg
     3961 ccgccggcgg ccgagaggcc cagcaggccc gcggcggctg ccccgaccgc cagcaccgcc
     4021 atcggggcga gcatcaccgc gggggattcg tggggatgcc cctcgtcgag gcgcgacgtc
     4081 cccagaaacg tcagcacgaa cgcccgggtt gcgtacagcg cggtgatccc cgccgtaacc
     4141 agcgccacga gccacagcca ttcgtgtccg gccccgtacg ccgccgccag aacctggtcc
     4201 ttggagaaga agccggataa cggcggcagc cccacgagcg ccagcccccc gatcccgaag
     4261 aagagcgtcg tccacggcat gacgcgcccc agcccgccga gccgcttcac gtcgatcaca
     4321 ccgcccgtgg cgtgcatcac gctgcccgca ccgaggaaca gcacggcctt gaagaacgcg
     4381 tgggtcacga ggtggaagat cgcaaccccg tacgcgccca cccccgccgc gaggaacatg
     4441 aacccgagct gggagatcgt cgagtaagcc aggacccgct tgaggtcgtc ctggcccagg
     4501 gccgaggtcg cggcgtagat cgccgtcacc gtccccacga tcgccaccgc gaacaaggcg
     4561 atctcggagg cctcgaacag cacgtgggcc cgcaccacga ggtacacacc ggccgtcacc
     4621 atcgtggcgg cgtggatcaa agccgagacc ggggtcgggc cctccatcgc gtcgggcagc
     4681 cagacgtgga gcgggagctg ggccgacttc cccacggccc ccgccaggag caggaacgag
     4741 atcgcggtcg cggtcccctc gggcaggccg cccgcagcgc cgaagacctc gtcgaaccgc
     4801 accgaccccg tcgcctggaa gatcaggaag atccccagcg cgaacccgaa gtccccgacc
     4861 cggttgacga tgaaggcctt cttcccggcg tcggccgcgg ccttccgctc gtaccagaac
     4921 gcgatcagca ggtacgagca gagtcccacg gcctcccacc ccaggtacag gacgacgaag
     4981 ttgtccgcca ggacgagcgc gcccatcgag aacacgaaga ggttgaggta cgcgaagtag
     5041 cgcgggtgcc gcgggtcgtc ccgcatgtag ccgatcgagt agacgtggat caggaacgag
     5101 acgccggtca ccgtcagcac catcacgagc gagagcgggt cgacgcggag ctcgaacggt
     5161 gcctcgaacg accccaccga gatccagtcg aacagccgaa ccgagaggct gcgcgcgtcc
     5221 ccggaccggc cgacgacatc cacgaacgcc gccagggccg ccacgaacgc gccgcccacg
     5281 gccccggagg cgatccaccc ggcggcgcgc tgcagcggcc tccccagcag gaggagcagc
     5341 accgcccccg ccaggggcag ggccagcgcc agccagatca agctcgccgg gccgtccgcc
     5401 gtcaccgctc cctccatcgc cggatcacca cttcagcagg ctgaggtcgt ccacgctggc
     5461 cgaggcccgc cgccggaaga tcgagacgat gatggccagg ccgaccacca cctcggccgc
     5521 cgccacgacc attgagaaga aggccagcac ctggccgtcc acctcgccca gctggcgagc
     5581 gaaggcgatc agcgtcaggt tcaccgcgtt cagctggagc tcgatcgcca tgaagatcac
     5641 gagcgcgttc cgacgcacga gcacacccac cgtgccgatc gtgaagagga tcccggacgt
     5701 ggcgagatac caggcgatcg ggatcctcac ctcggcacct ccgcggtcgt cgcgtcatcc
     5761 ccggaagcgg ccggctcccc cgcctccgga acgctcctgc tccgccgggc cagcaccatc
     5821 gccgccaccg ccgcgatgat cagcaggatc gaggtgacct cgaacgggaa gacgtagtcg
     5881 gtgaagagga cgcggccgac ctcctgcacg ttgcccgccc gccggggcat ctcctcggtc
     5941 gtccgctggc ccagccccac cccgatctgc acgacgaaca ggatcgcgat cacgaggaac
     6001 agaccgaggg cgatcgccag gggccgctgg ccgcgcaggc gctctcggag gctctcccgg
     6061 cggtccacgc cgaggaacat gatcacgaac aggaagagga ccatgatcgc gccggcgtag
     6121 acgatcacct ggaccgccgc caggaactcg gcgtccagca ggacgaagaa caccgccagc
     6181 gtgaactggt tgaggaccag gagcagcgcc gcgtggactg cgttccgctg gaacagcatc
     6241 agcgccgcgg cccccaccga gatcggggcc gtcacccaga acgtgatcag ctcgcccatc
     6301 tcatcgcccc tgatcggtcg tgggccccgt ccccaggcgt ggcggcggct ggagcaactg
     6361 ctccttctcc aggatcgcgt cctcccgcgt ggaccacgac agctcgaacc cgctcgtcag
     6421 ggtgagcgcc cgcgtggggc aggcctcgac gcacagcccg cacatgatgc agcggaggta
     6481 gttgatctgg tagatccggg cgtgccgctc ccccaccgag atcggccgct cggggtcgtt
     6541 gtcctcgccc tcgacgtaga tcgcgtcggc ggggcaggcc caggcgcaga gctcgcaccc
     6601 gatgcacttc tcgaggccgt cggggtagcg gttgaggagg tggcggccga tgtgcgaccg
     6661 gggcggaagc tccttgatct ccttggggta gtcgatggtg acgggcctgc ggaacatgct
     6721 ccggaaggtc acgccgaggc ccttgaggat ctcgatgatc atccggccct cgcctcctcc
     6781 tcgggcttcc gtgagaagag cggcgcgacg accgaggcga gcagggcaac acccgcggcg
     6841 atcgccgccc accgcaggaa ggtgtcgcgc ccgtagaggt ccgggaggat cacgatcgcc
     6901 ccggtggcca ccacccagat cagcccccag gggatcagca ccttccatcc gaagttcatc
     6961 aggcggtcgt agcggaaccg gggcagcgtg gcccggatcc agacgaacac gaagatgagc
     7021 gcgatcagct tcaggaggaa ccacagcagg ggccacagcc acgggaggaa gtcgaagacc
     7081 ggcccgcgcg gccccccgaa gaacacggtg accgccaccg ccgacaccgt gatcatgtgg
     7141 aggtactccg ccaggaagaa catcgcgaaa cggatccccg agtactcggt gtggaacccg
     7201 gccacgagct cggactcggc ctcggggagg tcgaacgggg cgcggttcgt ctcggccatc
     7261 gccgagatcc cgaagatgaa gaacgccggc gcttggggga tcacgttcca gatcgtgtcc
     7321 tggcgagcca cgatctcgga catctgcagc gtccccgagt acatcagcac ggccacgagc
     7381 cccagcccca gcgagatctc gtaggagacc atctgcgcgc tcgaccgcac cgcgcccagc
     7441 agggggtact tcgtcccgga cgaccacccg gccagcacga ccccgtagac gccgagcgat
     7501 ccgagggcca gcacgtagag gatcccgatg ttgagatcgg cgagctggaa cgggatctcg
     7561 cggccgaaca ggctgatcga ggtcccgaac gggatcacgg cgaaggcgag gaacgcgggg
     7621 atcagcgaga cgattggggc caggaggtag acggggcggt cggccgacgc gggcgtcagc
     7681 ccctccttga agaacagctt gatcccgtcg gcgagcgtga cgaggatccc gaagggtccc
     7741 gcgcggttcg ggcccaggcg cgtctgcata tcggccacga gcttccgctc ggcccagatc
     7801 gtcagcagga cggcgaccat cagcgcggcg aagaggatca ggacgcggat caggaggatc
     7861 agccagtcca cccagttccc cgcgtcgaag gcgctcatcc cgctccctcc agggcctcga
     7921 ccggctccgg ggccgcttcc agctccacgg ccgtgatcgt ggatccggtc agcagctcgc
     7981 tcatccgcag gccctcctgg ttgtagggga cgaagaccgc gccctcgtgg atgccgtcgg
     8041 tcacgacgac gggcagctcg gcctcccctc gctcggtccg gatcctggcc cggccgccgt
     8101 cgcgcacgcc gagccgctcc gcgtcggtcg ggtggacctc gaggaatggc tcgcggtgga
     8161 gggccgcctt cagctcgtcg gcgtccacgg agagtcggcc accgtccacg agcagggggt
     8221 aggtgaagag cgagaggccc tccacctcgc ggtccttcgt cagcgccggc acggacgccg
     8281 gcgccgggac ctcccgcggg gcgagcaggt cgcccagctc ggcctggacg tcctccaggg
     8341 agcggaagcc gaggtccgtc cccaggacct tggagagctc ctggaagatc cgccactcgg
     8401 gccgcgagag gccgacgggg tcacggaccg gcgccatccg ctggccgcgc ccctcccagt
     8461 cggtgaggtg cccggccttc tccaggaagg ccgcggcggg gatgaccgcg tcggcgtagg
     8521 gctcgtactc gcccaccgag atgtccacga tcaccgtgaa gccggcgtta gccagggcgc
     8581 ggcggacgag ctgctggtcg gggaagtcgg tgagggggtc cacgcccccg aggaagagga
     8641 ggtcgatctc ccgggcggcc gccgcctcga ggatctcgcg ggtgctgcgg cccggctcgg
     8701 ccgggagcga gccgccccag accgcctcca cctcggcccg ctcggcgggg tcgtcgaggc
     8761 ggcggccgcc cgggagcagg gcggggtgga cgcccgcgcg catcgcgccg cggtcgttcg
     8821 cgcgccgggt gaggtaggcg atcctcccgc ccacgtcggc cgcgagggag gcggcctgtt
     8881 ccagggcctc tgggctctcc gcgagccgcg ggcctgccag aaccacgagc ccctcccccg
     8941 cctcgcggag ggcgttggcc gcgtcggtgg agccggagcg gagctcggcc agcgccgcgg
     9001 cctcggcccc gggcgggcac aggacgtgct cggcgacgtc gagcgcggtg cggcgggggt
     9061 ggaggacgaa gaccttggcc ccgcccttgc gatgggcctt ccggatgcgc agatgcagga
     9121 tgggcagctc ctccctggtg tcgagcccca cgacgaggat cgccgcagcg cgctcgacgt
     9181 cgccgtaggt caccggcgtg ccgggggccg cctggacgcg ctccaggtcc cacgaccccg
     9241 tgcccacgcc catccggtgg tcgaggtcgt tcgtgcggaa ggccacgcgc gccagcttgg
     9301 ccatggcgta ggcgtcctcg tcggtgaggc gcgcgccgcc gaggacggcg gcgcgcgcct
     9361 cacccttcgc ccgcgccgcg atctccgcga acacctcggc gtgcgaggcc ggctcgaggc
     9421 ccggcacccg cagcatcggc gtctccagcc gctcccggtc ggggaaccgg aacgcgttcc
     9481 ggcccttgtc gcacagccag gcctcgttca cctccggttc gtccttcgcc aggtgccgca
     9541 cgacctcgcc ccgccgcgtg tccacgcgga tgctgcaccc gcacgcgcag tgcgggcaga
     9601 tcgagtcgcc tcgcttcacg tcgaagggcc gcgcggcgaa ccggtaggac gcgttcgtca
     9661 gcgcccccac cgggcagatc tggaccgtgt tcccgctgaa gggcgaggcg aagtcctccc
     9721 ccggcgagat cgagacctgc tcggccgccc cccgctcgaa cagctcgatg aaccgctccc
     9781 ccgagatctc gtcgcagaac cgcgtgcacc tcgcgcacag cacgcaccgc tcgcggtcca
     9841 gcaggacctg cggcgagagc tcgaccggct tctcgtagac ccgcttggga tcgatgtagc
     9901 ggctctcggc cggcccgaac gccagggtct ggtcctggag cggacactcg ccgccccggt
     9961 cgcagaccgg gcagtcgagc gggtggttga tcaggaggaa ctcgagctgg cctcgctggc
    10021 ccttggccgc gccctcgctc gtgttctggg tgcgcacgac catgtccggg gcgaccggcg
    10081 tcgtgcacga cgtcacgagc ttggcctgtc cctcgacctc gaccaggcac gtccggcacg
    10141 cccccaccgg gtcgaggtac gggtggtcgc agaagcgcgg gacctcgatc ccgatcgact
    10201 ccgccgcccg gatgatcagc gtcccctcgg ggaccgtgac ccgccgcccg tcgatcgtca
    10261 gcgtgacgct cacggcagga cctccgagat cgggatcgcg cccgccgcac cgcccgcccg
    10321 cggcgtggcg gcctcgagct tcggccgctc cttcctcggg caccccccca ccttcacgtg
    10381 ttccacgtac tcctcgcgga agtgcttgat cgagctgtcg atcggcgaga cggacccgtc
    10441 ccccagcgcg cagagcgact tgaagaggat gttgtcgccc acgtcgacca tcatgtcgag
    10501 gtcgtcgagc cgcccgcccc cgtgctccat ccggtggagg accttcgtca tccaccaggt
    10561 cccctcgcgg cacggcgtgc acttcccgca cgactcgtgg gcgtagaact gggtccagcg
    10621 cagcacggcg tccacgatgc agtcgtcctc gtcgaggacc acgaccgcgc ccgtgcccag
    10681 cagggacccc tgttcggaga tcgactcgaa gtccatcggg gtgtcgaggt ggtcgggcgt
    10741 gaggaacggc gtggacgacc cgccggggac ccaggccttc agcgccttcc cgccgcgcac
    10801 cccgccggcc agctcctcga cgatcacccg ggccggcgtc cccatcggcg cctcgtagtt
    10861 gcccgggcgc tccaccttgc cggagaggca gaagatcttc gtcccggtgg acttctcggt
    10921 gccgagcttc gcgaactcct cggcccccat gcggatcacg tgcgggacgg tggagagcgt
    10981 ctcgacgttg ttgatcacgg taggcgagcc gtagaggccg gccaccgcgg ggaagggcgg
    11041 acgcaggcgg ggctggcccc ggtagccctc gagcgactcg agcagcgccg tctcctcgcc
    11101 gcactcgtag gagcccgcgc cgcggtgcat cacaacgtcg aggtcgaagc cgctcccggc
    11161 gatccccttc cccaggaagc cgcggtcgta ggcctcggcg atcgcccgct cgacccggcg
    11221 ggccggctct aggaactcgc cccgcatgta gatgaacgcc tggtggcaac cgaccgcgta
    11281 ggaggcgatc gcgatcccct ccagcagctg atgcgggtcc cgctccatca gctcccggtc
    11341 cttgaacgtg ccgggctccc cctcgtccgc gttgcacgcg aggtagacgg gcttgcccgt
    11401 gtccttcggg atgaacgacc acttcacgcc ggtggggaag ccggcgccgc cgcgcccccg
    11461 gaggcccgag gccttcacct cctcgatcac atcgtccggc gacatcccga gcgccttccg
    11521 cagcgcctcg tagccgccgc cctcgaggta ggagtcgatc ttctccgatc cctcgatgcc
    11581 ccaccgggcc gtggtcagct tggggaagcg ctcgtcactc acggtcgtcc cccaggccgg
    11641 ccaggatgcg cgaggtcgcg ccgtggggcg cgggcttcgg gccgcgagcg gggggcgggg
    11701 gctcgccggc ccgcagggcc tcgatcagct cgacgacccc ctcctcgctc acgcggtcgt
    11761 agttgcagta gttgacctga accaccggcg cggcgtcgca ggccccgagg cactccacct
    11821 cctcgacctc gacgtcctcg tcgcccccgg ccgctccgcg ggcggcctcg tgcacgtccc
    11881 gggccccgag cagggcgcac gagacgttcg tgcagaccga caggagccat cgccccgccg
    11941 gctctcgccg gtacatcgtg tagtaggtgg cgaccgcctc cacctcggcc gtggtgagcc
    12001 cgaggagctc gcccacctcc cgcatcccct cgcgcgagag gtgcccctcc accgactgcg
    12061 ccaggtagag gaggggcagg agcgccgatc gcgagcgcgg gtagcgcgcc acgatcgact
    12121 cggcctgctc ccggaccggc ccctcgaaga ccatcagcgg tccaccccgc ccatcaccgg
    12181 atccacgctg gcgatcgagg cgatcacgtc ggccacgagc ccgccccgga cgatctccgg
    12241 cagcgcctgg acgtggacga acgaagggtc cttgatccgg acgcggtagg ggcgcacgcc
    12301 gccgtcggag acgacgtaga agccgagctc cccgcgcggc gactccacgc aggtgtagac
    12361 ctcgcccgcc gggaccttca cgccctgggt cacgagcttg aagtggttga tcagcgcctc
    12421 catcgactcc tccatgatgt ggcgcacgta ccgctccgag ttgccgatgc cgtcggggcc
    12481 gacctcgagc tgggccggcc aggcgatctt cgggtcgtcc accatcacgc ggcccccggg
    12541 cagccggtcg aggacctgct ccacgatctt cagcgactcg cggatctcgg ccacccggac
    12601 ctcgtagcgc gcgtagcagt cggcctcctc acgggtgggg atctcgaagt cgtacccctc
    12661 gtacagggga tcggtcgtcc ggagatcgtg cggcacgccg gccgcgcgga ggaggggccc
    12721 ggtgacggtc caggtccggc actgctccgc ggtgaggatc ccgacgccgc ggttccgctc
    12781 cacccagatc gggttgccgg agagcaggct ctcgtactcg tccaccttgc cgtccatatc
    12841 gacgatgagt gcccggatcc gctgctccgc cccctccggg aggtccatca ccagccctcc
    12901 gggacggatg tacgcgtggt tcatccgcag gcccgtgatc gcctcgaaga cgtcgaggag
    12961 cttctcccgc tctcggaacc cgtagagcat catggagagg gccccgagct ccaggccggt
    13021 cgtagccagc cacacgaggt ggctggcgat gcggccgagc tcgcacacca tcaccctgat
    13081 cgcctgggcc cttggcgggg cctcgatgcc gagcagccgc tccaccgagt gcgcgtacgc
    13141 cagctcgttg gagaacggcg agaggtagtc ggcgcgcgtg acgaacgtgg atccctgcgt
    13201 ccacgtccgg tactcgatgt tcttctcgat gccggtgtgg aggtacccga ccaccggctg
    13261 gcagtcgagc accgtctcgc cgtcgagggt cagcacgagc cgaaggaccc cgtgggtcga
    13321 ggggtgctgg gggcccatgt tgatgaccat gaggtcctcg gccaggcgct gggcctggac
    13381 gccctgggcc atctcggtcc ctccgagcgg ggtggggacg cggtccactc agagctcccg
    13441 ctggtccggg ggagggacgt acgcgccctt gtactgggtg gccacgccgc ccagcggctc
    13501 gtccttgcgg agggggtgcc ccacccactc ctcgggcagc tcgatccggc ggaggtcggg
    13561 atggccgtcg aacgtcacgc cgaagaagtc gtagacctcg cgctcgtgcc agttggccga
    13621 ggggtggacc tcggtcgcgg tggcgatccg gaggtcgtcc tcggggagcc cggccttcac
    13681 ccggacccgc atcccctggc ccgacgagag gtggtacgcc atccagatcc tcggctcgcg
    13741 ccccggccag tcggtgcacg agaggtccga gaggaagtcg aacccgaggt cgggctcgtc
    13801 gcgcaggaac cggagcgcgt ccacgagctc gtcgcggccg atcgtgatcg tcacctcgcc
    13861 ccgcgccacg agcacgtcgg ggaaccgagc gccgagacgc cctgccagct cgtccgggct
    13921 cacgtcaggg tcccccgctg ctggatcttc tcgtggagct ggaggatgcc gtacatcagc
    13981 atctcgggac ggggcgggca gcccggcacg tagacgtcca cgggcacgac ggtgtccacg
    14041 ccctggatca cgccgtagtt gttgaacatg ccgcccgacg aggcgcacac ccccatcgag
    14101 atgacccact tcggctcggg catctggtcg tagatctggc ggaggaccgg ccccatcttc
    14161 tggctgaccc ggccggccac gatcatgagg tccgcctggc gcggcgaggc ccggaagacc
    14221 tccatccccc accgggcgag atcgtgatgg ccggcgccgg tggccatcat ctcgatcgcg
    14281 cagcaggcca ggccgaatgt gaccggccac gtggactgct tcctcgccca gttgaccatc
    14341 ttggccaggg tgctcgtgag gattccctgg cggatctcct cctcctcggc ccggcgaacg
    14401 gcgtcgcccg cgcgcgcggg atcgctcatc gcgcccactc cagcccgtcc cgcttccaga
    14461 cgtagacgta ggccacgaac agcagcccga tgaagatcac catctccacg agcccgaaga
    14521 ggcccagctg gcggaaggcc accgcccacg ggaagaggaa gatcgtctcg atgtcgaaga
    14581 tgatgaacag catcgcgatg acgtagaact tcacggggaa gcgctcgggg ggctcgtgct
    14641 cggggacgat cccgcactcg tacggagcca tcttggccgg ggtgggcttc cgcggccgca
    14701 ggagggccga cgcggcgagg gaggccacgg cgaacacggc cgccagcccg gccaggatca
    14761 cgatcgggag gtactcgacc agcatcccga tcgaggctag aagctgtcag aggacacgcg
    14821 caaatccccc tcgactcggc gccagacgcg tcagcaggtg gatcagacga tcgtcgacgt
    14881 ccccgtcccg gccgtccgtg aggttcgcca tcagcttgaa gaggaaccgc atcagccgct
    14941 ctcgggggag gccgtaccgg acggcggtcc gcatcacggt ggggctgccg atcgcccgga
    15001 cgaaggcccg ccccatcgag aagtacttgc cgtaccgctg ccggagcacc gccgggtaga
    15061 cctgggccag gcccggccgg tcgaggacga gggcctcgtg gatcagctcc gccgccagac
    15121 gccccgactc catcgcgtag gcgatcccct ccccgttgaa ggggttgatg atgccgccgg
    15181 cgtcgcccgc cacgagcagc ccggggaccg cgagcggcgc gcggttcatc cccatgggca
    15241 gagggcccga cagcagcctc ccctcggcgg tctcctcggt gatgccgagc tcgggaggga
    15301 ggcggcggag gaagacctcg tacaccttct tggccgagag gtgtcggaag tcgcgggccg
    15361 tgttcagcag tccggcgccc acgttcacga tcccgtcgcc cacggggaag atccagccgt
    15421 agcccggcag gaagccaccc tgatgctcga ggtcgagcca ggagaggaag tgccgctcgt
    15481 tcgccagggg gctccggaag tagcggcgcg ccgcgatccc gatcggac
//
LOCUS       DASUTZ010000222         7546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_854360, whole genome shotgun sequence.
ACCESSION   DASUTZ010000222 DASUTZ010000000
VERSION     DASUTZ010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7546
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854360"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..524)
                     /locus_tag="VF097_09730"
     CDS             complement(42..524)
                     /locus_tag="VF097_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6263112.1"
                     /translation="MTTITSSIEVDVPLSTAYNQWTQFEEFPQFMEGVERVEQLDDTM
                     LRWTASIAGQERTWEAKILTQEPDRQVSWAAVGDVVHAGTVTFHELAPDRTRVNVEME
                     YEPQDLVEKAGDALNLIERRVDGDLERFKEFIESRGVESGAWRGRVEGGEEMSGGPTV
                     "
     gene            618..1406
                     /locus_tag="VF097_09735"
     CDS             618..1406
                     /locus_tag="VF097_09735"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEX6263113.1"
                     /translation="MLGAIPYRSLPEIPLGPISLKTFGLMVALGVLVGVALAARHAGS
                     RGHSGEEVTRLAVVMVVTGFVGARLAWVLSHLDQIESPLDVLAVWEGGLAFTGGFVAA
                     TAVAVFALRRWPRTKRWEVADGMALGLTVGLAIGRLGCLAVGEHLGGPTGFFLGMRYE
                     GGATIEGPLEVGVVYHSTALYEALHLVALAALLWWLLRPGRVPPGTAITVFALWYGTA
                     RFLTDFLRAYDREVLGLTGAQWACLALLVAGAWAALRLRRPAPA"
     gene            1574..2587
                     /locus_tag="VF097_09740"
     CDS             1574..2587
                     /locus_tag="VF097_09740"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX6263114.1"
                     /translation="MRIRTTLAAAALALLAAGCGGEGGSDGGGGLTGEIVVSGSSTVE
                     PISGLNAEKFAGENPDVAISVEGPGTGDGFELFCGGETDISDASRPIADDESELCAQN
                     GIEYTELEVAIDGIAVLTSPENEAVSCVGFGDVYALVGPESEGLDSWADAQGLAAEIS
                     SDEMPTPAADAGLPDAPLEITAPGEESGTYDSFIELTFEGFAEDERGQDPVTRPDYVA
                     SADDNVIIEGIAGSPSSFGWVGFAFYRNNEDVVKALEVDAGSGCVAPSEQTIADGSYP
                     VSRSLYIYVNDARAAESEALAAFVDFYVSDEGFASVAEVGYVDLPPERIEATRAAWEE
                     ARP"
     gene            complement(2626..3606)
                     /locus_tag="VF097_09745"
     CDS             complement(2626..3606)
                     /locus_tag="VF097_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015406707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX6263115.1"
                     /translation="MPLALVIFLTLLFQFANGWTDAPNAIATVVSTRVLRPYAAVVMA
                     ATLNLVGALVTGTLVAETIAQGIVSPDVVTLETIGAAVFAAALWAMGAQVFGMPSSET
                     HALVASLLGAGFAAGGLNALEWSGTQKVVYSLFTSPVIAFFGGLLLMVGIYWAFRRAR
                     PRTVRRTFGKGQVLSAAFMAFSHGSNDGQKGAGIIALALFLNGNLPELEIPLWVLFVV
                     ATTIGVGTAVGGWRVIRTIGLRMTKLEPVNGFAAETSAAMIILAGSRLGIPLSTTHAI
                     GSSIMGVGATRRLSAVRWGLAGEVVGTWILTWPACMLLSFAMAKLFHAIF"
     gene            complement(3611..4228)
                     /locus_tag="VF097_09750"
     CDS             complement(3611..4228)
                     /locus_tag="VF097_09750"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEX6263116.1"
                     /translation="MKFRLIPREESFYDLFIKQAEILVQATEVFRGIAAEFTDVKRRA
                     RRVHDLEHEGDEVTHEVMRRLNTTFVTPIDREDIHELASNMDDVLDHMDAAIDFLVLH
                     NIDRPIQEMKAQIDLLSEAAKATLEAMQTLPAFDRLSRYWIEINRLENEADRVYRQGV
                     ADLFSGEFEAMEVLRWKDIIDQLERAMDKLEDVANTLEGIALKQA"
     gene            4380..5330
                     /gene="pstC"
                     /locus_tag="VF097_09755"
     CDS             4380..5330
                     /gene="pstC"
                     /locus_tag="VF097_09755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX6263117.1"
                     /translation="MRSLSGSGSAARIEALRGDPRRRRRERAVGFVFLAAAALSVVVS
                     AAIVLSLAGEAASFVSKVDPGSLWSEGWFPRRNLFDLRTIVVGTLMVAGVAMLVAAPL
                     GLLTAMYLSELAIPRARRLLKPVVEVLAGIPSVVLGYFALTFINPELVQRFLGGTSPF
                     SMLGAGLAVGILVTPLVASVAEDALHAVPQALREASSGLGAKRTTTTLRVVFPAAVSG
                     IVAALILGLSRAIGETMIVAIASGSTGGSLLSFDIVGPGQTMTGAMTALAVGSDRVAV
                     GGGGTTVNPVDALFFVGLLLFVMTFALNVVSERLVRRMRV"
     gene            5330..6271
                     /gene="pstA"
                     /locus_tag="VF097_09760"
     CDS             5330..6271
                     /gene="pstA"
                     /locus_tag="VF097_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005580195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX6263118.1"
                     /translation="MGAARTLGATPARVAVEDALHGKRLDLGGILLHGMLLGSLLLTL
                     TVLAMLLLEVVTSAWSVFAERGLGVVTSDLSSIASRVGVWQGIQGSLWLMAFVVLFSF
                     PLGIGAAIYIEEYARDTRLTRFINANIRNLAGVPSVVYGLLGLAIFVRVLGGLTGGRS
                     LISGGMTLAILVLPIVIITASEALRAVPGEIREAAYGVGATRWETIRSHVLPYAAPGV
                     FTGTILALARAFGETAPLLMVGVATGFLSLGGGGLVDQLQGPYTALPAIIFRWSKLPQ
                     ADFREGLAAAAIVVLLVVLLAVNALAIILRNRYERRW"
     gene            6276..7100
                     /gene="pstB"
                     /locus_tag="VF097_09765"
     CDS             6276..7100
                     /gene="pstB"
                     /locus_tag="VF097_09765"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017615596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX6263119.1"
                     /translation="METRLERPEPRVEVRSAGEAILDLRNVSVCYGDVRAVDDITLAI
                     RERQITALIGPSGCGKTTVLRSLNRMNDLIEGARVDGAILYHGVDLYDERVDPVEVRR
                     RIGMVFQKPNPFPKSVFENVAFGPRISGSEGDLEERVRWALTKAALWDEVKDTLRGPA
                     MALSGGQQQRLCIARAIAVEPDVILMDEPCSSLDPIATLKIEDLMRELVSDYTIVIVT
                     HNMQQAARVSDRTAFLTIDLEDGSRAGRLVEVDDTEVIFTNPSDPRTEDYVTGRFG"
     gene            7097..>7546
                     /locus_tag="VF097_09770"
     CDS             7097..>7546
                     /locus_tag="VF097_09770"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HEX6263120.1"
                     /translation="MSAEPRRRFHDELAGLEAAIGEMGERAERAIGEAMAALTTGGTG
                     DAVVEADDAIDDRYLDVERRILDLLARQTPVASDLRLVSAILHINLHLERVGDIAVNI
                     AKIANATRDLPRSEPILAALVEMGDVVRPMLRASMEAFARRDLDLARR"
BASE COUNT         1018 a         2594 c         2729 g         1205 t
ORIGIN      
        1 gggccgccgc cgtcccgatg ggacggcggc gccccgcggc ctcagacggt cggcccgccg
       61 ctcatctcct cgccgccctc gacgcggcct cgccatgcgc cggactcgac gccgcggctc
      121 tcgatgaact ccttgaaccg ctcgaggtcg ccgtccacgc gacgctcgat caggttgagc
      181 gcgtcgccgg ccttctccac caagtcctgc ggctcgtact ccatctccac gttgacccgc
      241 gtccggtcgg gcgcgagctc gtggaacgtc accgttccgg cgtggacgac gtcgcccacg
      301 gccgcccagg acacctggcg gtccggctcc tgggtgagga tcttcgcctc ccacgtgcgc
      361 tcctggccgg cgatgctcgc cgtccagcgc agcatcgtgt cgtccagctg ctcgacgcgc
      421 tccaccccct ccatgaactg cgggaactcc tcgaactggg tccactggtt gtaggcggtc
      481 gacagaggga cgtcgacctc gatcgagctc gtgatggtcg tcatcggcac ccacctcctt
      541 gtccctccct cgatacccga ggctggtggt cggaaaccgg agcaggggcg gctaccctgg
      601 aggccgaagg gggggacatg ctgggggcga tcccgtaccg atctctgccg gagatcccgc
      661 tggggccgat ctcgctgaag acgttcgggc tgatggtggc gctcggcgtg ctcgtcgggg
      721 tggcgctggc cgcgcgccac gccggcagcc gcggccactc gggcgaggag gtgaccaggc
      781 tggccgtggt gatggtggtc acgggcttcg tgggggcccg gctggcctgg gtgctctccc
      841 acctcgatca gatcgagtca ccgctcgacg tcctggcggt gtgggagggg ggcctggcct
      901 tcaccggtgg gttcgtggcg gctaccgcgg tggccgtgtt cgccctccgg cggtggcccc
      961 ggacgaagcg ctgggaggtc gccgacggga tggcgctcgg cctgacggta gggctggcga
     1021 tcggccgcct ggggtgcctg gccgtcggcg agcatctcgg cggcccgacc ggcttcttcc
     1081 tggggatgcg ctacgaggga ggcgccacga tcgaggggcc gctcgaggtg ggggtcgtgt
     1141 accacagcac cgccctctac gaggcgctcc acctagtggc gttggccgcc ctcctgtggt
     1201 ggctgctgcg ccccggacgg gttccccccg ggacggcgat cacggtcttc gcgctctggt
     1261 acggaaccgc gcggttcctc accgacttcc tccgggccta cgaccgggag gtgctcgggc
     1321 tcacaggcgc ccagtgggct tgcctcgcgc tgctcgtcgc gggagcgtgg gccgctctcc
     1381 gcctgcgccg gcccgcccct gcctgatctt tcacctcgcg ttcaccctct cgtcgaggtc
     1441 ccgtcgacga cggctcctag gctcggtcgc gtggagagag tgacgaacgc cgagcggccg
     1501 gcggccgccc gatcagcgcg cggaggcaag cggaccggcc tctccacccg cgcgcgctcg
     1561 aggagggatc tgcgtgcgca tccggacgac cctggcggcc gcggccctgg cgctcctcgc
     1621 cgccgggtgc gggggggaag gcggaagtga cgggggcggt ggtctcaccg gggagatcgt
     1681 cgtctccggc tcctccaccg tggagccgat ctcggggctg aacgccgaga agttcgccgg
     1741 ggagaacccg gacgtggcga tcagcgtgga ggggcccggg accggggacg gcttcgagct
     1801 gttctgcggc ggcgagaccg acatctccga cgcatcgagg cccatcgcgg acgacgagtc
     1861 cgagctctgc gcgcagaacg ggatcgagta caccgagctc gaggtggcga tagacggcat
     1921 cgccgtcctg acctcccccg agaacgaggc cgtctcctgc gtgggcttcg gcgacgtcta
     1981 cgcgctcgtc ggcccggagt cggaggggct cgactcgtgg gccgatgccc agggactcgc
     2041 cgcggagatc tcctccgacg agatgccgac cccggccgcc gatgccgggc tcccggacgc
     2101 gccgctggag atcacggccc ccggtgagga gtccgggacc tacgactcgt tcatcgagct
     2161 gaccttcgag ggcttcgccg aggacgagcg ggggcaggac cccgtgaccc gccccgacta
     2221 cgtcgcctcg gccgacgaca acgtcatcat cgagggcatc gcgggctcgc cctcctcgtt
     2281 cggctgggtg ggcttcgcct tctaccggaa caacgaggac gtggtgaagg cgctcgaggt
     2341 cgacgcgggg agcggctgcg tcgcgccgtc ggagcagacg atcgccgacg gctcctatcc
     2401 ggtctcccgc tcgctgtaca tctacgtgaa cgacgcgagg gcggccgaga gcgaggcgct
     2461 cgcggcgttc gtcgacttct acgtctctga cgaggggttc gcctccgtgg cggaggtcgg
     2521 ctacgtggac ctgcccccgg agcggatcga ggccacccgg gcagcctggg aggaggctcg
     2581 gccctgacgc cgggcgccgg ctgctgcgca tccggcgccc cctcctcaga agatggcgtg
     2641 gaagagcttg gccatggcga agctgagcag catgcaggcg ggccaggtca ggatccaggt
     2701 gccgacaacc tcgccggcaa gaccccacct caccgcggac agtctccgcg tggcccccac
     2761 gcccatgatc gaggacccga tcgcgtgggt ggtcgagagc gggatcccga ggcgggaccc
     2821 cgccaggatg atcatggccg cggaggtctc tgcggcgaac ccgttcaccg gctccagctt
     2881 cgtcatccga aggccgatcg ttcggatcac ccgccacccc ccgacggcgg tccccacccc
     2941 gatcgtcgtt gccacgacga acaggaccca gagggggatc tcgagctccg gcaggttgcc
     3001 gttgaggaag agcgccaggg cgatgatccc cgcgcccttc tgtccgtcgt ttgacccgtg
     3061 gctgaacgcc atgaaggccg cggagaggac ctgccccttg ccgaacgtcc ggcggacggt
     3121 cctgggccgg gcccggcgga acgcccagta gatgccgacc atgagaagca gccccccgaa
     3181 gaacgcgatc acggggctgg tgaagaggct gtagacgact ttctgggtcc cgctccactc
     3241 cagcgcgttc aggcctcccg ccgcgaagcc cgcgccgagg agcgaggcca cgagggcgtg
     3301 ggtctcgctg gagggcatcc cgaagacttg ggcgcccatc gcccacagcg cggcggcgaa
     3361 gacggcggcg ccgatcgtct cgagcgtcac gacgtcgggg ctcacgatgc cctgggcgat
     3421 cgtctccgcc acgagcgtgc cggtgacgag cgcgcctacc aggttgaggg tggctgccat
     3481 gacgaccgcc gcgtagggac ggagcacgcg cgtggacacg acggtggcga tcgcgttggg
     3541 ggcgtccgtc cagccgttgg cgaactggaa gaggagcgtc aggaagatga ccagcgcgag
     3601 cggcatccgg tcaggcctgc ttcagcgcga tcccctcgag ggtgttcgcc acgtcctcga
     3661 gcttgtccat ggccctctcg agctggtcga tgatgtcttt ccagcggagg acctccatcg
     3721 cctcgaactc cccggagaag aggtccgcga cgccctgccg gtacacgcgg tccgcctcgt
     3781 tctccagccg gttgatctcg atccagtagc gggagaggcg gtcgaaggcg ggcagcgtct
     3841 gcatcgcctc cagggtggcc ttggctgcct ccgagagcag gtcgatctgc gccttcatct
     3901 cctggatcgg ccgatcgatg ttgtggagga cgaggaagtc gatcgccgcg tccatgtggt
     3961 cgaggacgtc gtccatgttg ctggccagct cgtggatgtc ctcgcgatcg atcggggtaa
     4021 cgaaggtcgt gttgaggcgg cgcatcacct cgtgtgtgac ctcgtccccc tcgtgctcga
     4081 ggtcgtgcac ccggcgggcc ctccgcttca cgtcggtgaa ctcggcggcg atgccccgga
     4141 acacctcggt ggcctggacc aggatctcgg cctgcttgat gaagaggtcg tagaaggact
     4201 cctcgcgtgg gatcagacgg aacttcacgc agcctcccct ccccgccgca tgcgactcgg
     4261 cgccgcgcgc atggtaccgg atgcggggtc gtctcccaac aggccggcct ccgccatgtt
     4321 cacggaccgt tcaccccgcg tccatcccgg agcctcccgg cgtccctacc gtgaggcccg
     4381 tgaggtcgct gagtgggtcg gggtccgcgg cgcggatcga ggccctgagg ggggatccac
     4441 gtcgccgccg ccgcgagcgg gcggtcggct tcgtgttcct cgccgcggcc gccctctcgg
     4501 tcgtggtgag cgcggcgatc gtcctctcgc tggcgggcga ggcggcctcg ttcgtgtcga
     4561 aggtcgaccc gggctcgctc tggtcggagg ggtggttccc acgcaggaac ctgttcgacc
     4621 tccggacgat cgtcgtcgga acgctcatgg tggcgggggt ggcgatgctc gtcgcggccc
     4681 ccctggggct ccttacggcc atgtacctgt ccgagctcgc gatcccccgg gcacgccggc
     4741 tgctgaagcc ggtcgtggag gttctggcgg ggatccccag cgtggtgctc ggctacttcg
     4801 ccctcacctt catcaacccc gagctcgtcc agcggttcct cggcgggacc tcgcccttca
     4861 gcatgttggg ggcggggctc gcggtgggca tcctggtaac cccgctcgtg gcgtcggtgg
     4921 cggaggacgc gctccacgcg gtcccgcagg cgctgcggga ggcctcctcc gggctcgggg
     4981 cgaagcggac gacgaccacg ctgcgggtcg tgttccccgc cgcggtctcc gggatcgtgg
     5041 ccgcgctgat cctgggcctc tcccgcgcca tcggggagac catgatcgtg gcgatcgcct
     5101 ccggctctac gggaggctcc ctcctctcct tcgacatcgt ggggccgggg cagacgatga
     5161 cgggggcgat gaccgccctg gccgtgggat ccgatcgggt ggcggtgggt gggggaggga
     5221 ccacggtcaa cccggtggac gcgcttttct tcgtcggcct cctgctcttc gtcatgacct
     5281 tcgcgctcaa cgtggtcagc gagcggctcg ttcggcggat gagggtctga tgggagcggc
     5341 tcgaacgctg ggggccacgc cggcccgggt cgcggtcgag gacgctctcc acgggaagcg
     5401 gctggacctc ggcggcatcc tcctccacgg gatgctcctc ggctcgctcc tcctgacgct
     5461 cacggtgctg gcgatgctcc tcctggaggt ggtgacgtcg gcgtggtcgg tgttcgcaga
     5521 gcgaggcctg ggggtcgtga cgtcggatct ctcgtcgatc gcgtccaggg tcggggtctg
     5581 gcaggggatc cagggctcgc tgtggctgat ggccttcgtc gtgctcttct cgttcccgct
     5641 ggggatcggg gccgcgatct acatcgagga gtacgcgagg gatacgaggc tcacgcggtt
     5701 catcaacgcg aacatccgca acctggccgg cgtgccgtcg gtcgtctacg ggctgctcgg
     5761 gctggccatc ttcgtgaggg tcctgggagg cctcaccgga ggtcggagcc tcatctccgg
     5821 ggggatgacc ctcgccatcc tcgtcctccc gatcgtcatc atcaccgctt cggaggcgct
     5881 gcgggccgtg ccaggcgaaa tccgggaggc ggcctacggc gtgggcgcca cccgctggga
     5941 gacgatccgc agccacgttc tcccctacgc cgcccccggc gtcttcaccg gcacgatcct
     6001 ggcgctggcc agggccttcg gggagaccgc acccctcctg atggtcgggg tggccacggg
     6061 gttcctctcg ctgggagggg gcgggctcgt cgatcagctc cagggaccct acacggccct
     6121 cccggcgatc atcttccgct ggtcgaagct cccccaggcc gatttccggg aggggctggc
     6181 cgcggcggcg atcgtcgtgc tcctggtcgt gctcctggcg gtcaacgcgc tggcgatcat
     6241 cctgaggaac cgatatgagc ggaggtggtg agccggtgga gacgcggctg gagcgtcccg
     6301 agccccgggt ggaggtccgg tccgctgggg aggcgatcct ggacctgcgg aacgtctcgg
     6361 tctgctacgg ggacgtgcgc gcggtcgacg acatcaccct cgcgatccgg gagcgccaga
     6421 tcacggccct gatcgggccc tcggggtgcg ggaagaccac cgtgctccgg agcctgaacc
     6481 ggatgaacga cctgatcgag ggcgcccgcg tggacggggc gatcctgtac cacggggtcg
     6541 acctgtacga cgagcgcgtc gaccccgtcg aggtccggag gcgcatcggc atggtgttcc
     6601 agaagccgaa ccccttcccg aagtcggtct tcgagaacgt ggcgttcggc ccccggatct
     6661 cgggctccga gggggacctc gaggagcgcg ttcgatgggc cctgacgaag gcggcgctct
     6721 gggacgaagt gaaggacacg ctgcgggggc ccgcgatggc gctctccggc gggcagcagc
     6781 agcgcctgtg catcgcccgg gcgatcgccg tggagcccga cgtgatcctg atggacgagc
     6841 cgtgctcgtc gctcgacccc attgccaccc tgaagatcga ggacctgatg cgcgagctgg
     6901 tgagcgacta cacgatcgtg atcgtcaccc acaacatgca gcaggccgcc cgagtcagcg
     6961 accggaccgc cttcctcacg atcgacctgg aggatggcag ccgcgcggga cggctggtcg
     7021 aggtcgacga caccgaggtc atcttcacca acccctccga tccgaggacc gaggactacg
     7081 tcacggggcg gttcggatga gcgcggagcc gaggcgccgc ttccacgacg agctggccgg
     7141 cctcgaggcg gcgatcggcg agatggggga gcgcgcggag cgggcgatcg gggaggcgat
     7201 ggcggcgctg accacgggcg ggacgggcga cgcggtggtc gaggcggacg atgccatcga
     7261 cgatcggtac ctggacgtcg agcggcgcat cctcgacctc ctggcgcggc agacgcccgt
     7321 ggcctccgac ctccgcctcg tctccgcgat cctccacatc aacctccacc tggagagggt
     7381 gggggacatc gcggtcaaca tcgctaagat cgcgaacgcc acgcgcgacc tgccgcgcag
     7441 cgagccgatc ctcgccgccc tggtcgagat gggggacgtg gtccggccca tgctccgagc
     7501 gtcgatggag gccttcgccc ggcgcgacct ggacctggcc cgccga
//
LOCUS       DASUTZ010000223         2219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_985050, whole genome shotgun sequence.
ACCESSION   DASUTZ010000223 DASUTZ010000000
VERSION     DASUTZ010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985050"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..351)
                     /locus_tag="VF097_09775"
     CDS             complement(46..351)
                     /locus_tag="VF097_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263121.1"
                     /translation="MPRGASCTLQGTIVGGNVFVKRGAVLRAIGARIEGNVQAKRARN
                     VIVRAVDGDVQAFSNRGGVVIRGNRIDGNLQCRSNRPAPVGGNNRMSGNKEGQCRRL"
     gene            661..1014
                     /locus_tag="VF097_09780"
     CDS             661..1014
                     /locus_tag="VF097_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008355893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6263122.1"
                     /translation="MGRPVVHFEIGVEDPERSERFYRELFGWTMQEAAGGYRLVLTDG
                     DAGIGGGLMPTREAVPTYVTIYVDVEDLEATLLAAEKLGGKTLVEPMPIEGVGRFAMF
                     TDPDGNMVGILAQKE"
     gene            1077..>2219
                     /locus_tag="VF097_09785"
     CDS             1077..>2219
                     /locus_tag="VF097_09785"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018395927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX6263123.1"
                     /translation="MMPTRTETDSMGAIDVPADRYWGAQTQRSLHHFSIGDDRMPTEV
                     VRAFGILKKAAALVNMDLEKLDRERGGLIVRAADEVVDGALDDHFPLFVWQTGSGTQS
                     NMNANEVIANRAIELAGGELGSKEPIHPNDHVNMSQSSNDTFPTAMHVAAARAVTERL
                     LPSLRALRDALDAKAKEFSDIVKIGRTHLQDAVPLTLGQEFSGYVAQLDDDLERIEES
                     LDGLHELAIGGTAVGTGLNAPEGFGEMAAARIAELTGLPFRAAPNAFAALAAHDALVH
                     ASATLRTLAVSLMKVANDIRWLGSGPRSGLGELELPENEPGSSIMPGKVNPTQAEALT
                     MVCVQVMGNDAAVAIAGSQGNFELNVFKPVMIRNFLHSVAILADAMG"
BASE COUNT          335 a          809 c          750 g          325 t
ORIGIN      
        1 cggccccgcg gatgcggggc cgccgccgcc ccctcacgcc tcagctcaga gccttcggca
       61 ctggccttcc ttgttcccgc tcatgcggtt gttcccgccg accggagccg gcctgttcga
      121 cctgcactga aggttcccat cgatgcggtt gccgcgtatc acgaccccgc cccggttgga
      181 gaacgcctgg acgtccccgt ccacggcccg gacgatcacg ttcctcgccc tcttggcctg
      241 gacgttgccc tcgatgcgag cgccgatcgc ccggaggacc gctccgcgct tgacgaagac
      301 gttccctccc acgatcgtgc cctgcagggt gcagctcgct ccccgcggca ccctgatgtt
      361 gtcgccacgg tcttcggtcc gatcaccccc cggcatacga cgtcatcggc gttcaccgca
      421 gggcgggcgc cagcccagga ccttcgccgt cgccagcacc gtgcagcacc agtctgcgca
      481 tcgacttcct cctcaggtgg gcctgaccac accctctccc tacccggtgc gtcgatcgtg
      541 agacccgcgt gagaggtcgc tcatgggccg accggcccgg gcggcgtggg tccccctccc
      601 ctggcccccg cgcctccgag gcgggaccct ggtcaccgca cggaggagga ggtgcgaccc
      661 atgggacgtc ccgtcgtgca cttcgagatc ggtgtcgagg acccggagcg ctcagagcgc
      721 ttctaccgcg agctcttcgg atggacgatg caggaggccg ccggcggcta ccgcctggtc
      781 ctgaccgacg gcgacgccgg gatcggaggc gggctgatgc ccacccgcga ggcggtgccc
      841 acgtacgtga cgatctacgt ggacgtggag gatctcgagg ccacgctcct ggcggccgag
      901 aagctgggag gcaagaccct cgtggagccg atgccgatcg aaggcgtcgg tcgcttcgcg
      961 atgttcaccg atcccgacgg caacatggtg ggtatcctgg cccagaagga gtgagcgcct
     1021 gcgggtcccg gcccgcggcg cctagaatcg gtcctcggag acaccacagg gggtcgatga
     1081 tgccgacgcg caccgagacc gacagcatgg gggcgatcga cgtccccgcc gaccggtact
     1141 ggggggcgca gacgcagcgc tcgctccacc acttctcgat cggcgacgac cggatgccga
     1201 ccgaggtggt ccgggcattc gggatcctca agaaggccgc cgcgctcgtg aacatggatc
     1261 tcgagaagct cgaccgcgag cgcggggggc tgatcgtccg ggccgccgac gaggtggtcg
     1321 acggggccct cgacgaccac ttccccctct tcgtctggca gacgggctcg ggcacccagt
     1381 cgaacatgaa cgcgaacgag gtgatcgcga accgggcgat cgagctggcg ggcggcgagc
     1441 tgggctcgaa ggagccgatc caccccaacg accacgtcaa catgtcgcag tcctcgaacg
     1501 acaccttccc cacggcgatg cacgtggccg cggcccgggc cgtgaccgag cgactgctcc
     1561 cgagccttcg ggcgctccgc gacgcgctcg acgccaaggc gaaggagttc tcggacatcg
     1621 tgaagatcgg gcggacccac ctccaggacg cggtcccgct cacgttgggc caggagttct
     1681 ccggctacgt ggcccagctc gacgacgacc tggagcggat cgaggagtcc ctggacgggc
     1741 tccacgagct ggccatcggc gggacggcgg tcgggacggg gctgaacgcg cctgagggct
     1801 tcggcgagat ggccgccgcc aggatcgccg agctgacggg gctccccttc cgggcggctc
     1861 cgaacgcctt cgcggccctc gcggcccacg acgccctcgt tcacgccagc gccaccctcc
     1921 ggaccctggc ggtctccctc atgaaggtcg ccaacgacat ccgctggctc ggctccgggc
     1981 cccgctcggg cctcggggag ctcgagctcc ccgagaacga gcccggctcc tcgatcatgc
     2041 cgggcaaggt gaaccccacg caggccgagg ccctgacgat ggtctgcgtc caggtgatgg
     2101 ggaacgacgc ggccgtggcg atcgccggct cccagggcaa cttcgagctc aacgtcttca
     2161 agccggtgat gatccggaac ttcctgcact cggtggcgat cctggccgac gcgatgggg
//
LOCUS       DASUTZ010000224         2531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1113814, whole genome shotgun sequence.
ACCESSION   DASUTZ010000224 DASUTZ010000000
VERSION     DASUTZ010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113814"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1484
                     /locus_tag="VF097_09790"
     CDS             <1..1484
                     /locus_tag="VF097_09790"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX6263124.1"
                     /translation="LVILLLVPLGITLVLRSVPTLDPIFESPRFHIVVVSAIALCALI
                     VALLTAISAAKAREAAPVLMAMGCLAVGVLMFAHGMTTPGIGGRGFSFWVVRFPVLAL
                     AGFAACLALAAAPDRATKRLVGRFPRGSLLAAATLLSAFSLVLIARPHALSGLRPIHG
                     EEAARWVLIGGTTVTLLGVGAVHWHRWRLGRDRVQLGLMSAAWLSANAAVALEVGHLW
                     RVSWWDYHVYLLAGFAAAAWSVTTQSRQTRSVREALATVSIRDPIQLVSRGHPEALDA
                     LVGAVEAKDSHMRGHSARVAEVAMRIGLRMDMGADELRGLAQGAFLHDIGKIGVPDDV
                     LHKRDRLTREDWAWIEQHPVIGGEMAGRAPSLHDSLAVIRHHHERWDGSGYPDRLAGD
                     EIPIAARIATVADVWDALTSDRSYRPAWPVDRALAYIAGAAGHLFDPVAVDAFLDLLA
                     HRGLWPDRLQPDVDTLASMAEACHPRTNGHAAGVTAAEPGRSA"
     gene            complement(1481..1780)
                     /locus_tag="VF097_09795"
     CDS             complement(1481..1780)
                     /locus_tag="VF097_09795"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX6263125.1"
                     /translation="MVVTVVAAHVAEERGSEFRHSFAGATADLPPGIVQTFLLHVPES
                     HEWRILTIWRSREELEAYRAAVEVPEAIRMFRAVGSEPVMGEFDVVEQAAETAGA"
     gene            1941..2492
                     /locus_tag="VF097_09800"
     CDS             1941..2492
                     /locus_tag="VF097_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263126.1"
                     /translation="MTRLFRFGGYFASIVLILFGIGAAVLGTLGFLEVRDTIAQESIT
                     ATPDAAEIEGVNLEPGQEIRTGGEAKEFAEIIRSHALEATGGRAFAEIPRYITETGEE
                     TDDEAAAATTEEGRPVENPVRGIWNTATAFTTALNTAYLAEQIALFTIAMGVALVLVG
                     IGFLVLTIAALRGPVFERHRDTT"
BASE COUNT          339 a          927 c          885 g          380 t
ORIGIN      
        1 tgctcgtgat cctcctcctc gttcctcttg ggatcaccct ggtcctccgt tcggtcccca
       61 cgctcgatcc catcttcgag tcgccccgct tccacatcgt ggtcgtcagc gcgatcgccc
      121 tctgcgcctt gatcgtggcg ctcctcacgg cgatctccgc cgccaaggcg agggaggcgg
      181 cgccggtgct catggccatg gggtgcctcg cggtcggcgt cctgatgttc gcccacggga
      241 tgaccacacc cgggatcggg ggccgcggct tcagcttctg ggtggtccgc ttcccggtcc
      301 tggccctggc cggcttcgcc gcctgcctcg ccctggccgc cgctccggac cgggccacca
      361 agcgactcgt cgggcgcttc cccaggggct cgctcctggc ggccgcgacc ctgctctccg
      421 cgttctcgct cgtcctcatc gctcgccctc acgcgctctc gggcctgcgt cccatccacg
      481 gcgaggaggc cgccagatgg gtcctcatcg ggggcaccac ggtgacgctg ctcggcgtgg
      541 gggccgtcca ctggcatcgc tggcggctgg ggagggaccg cgtccagctc gggctgatgt
      601 cggcggcgtg gctctcggcc aacgcggcgg tcgcgctcga ggtggggcac ctctggcgcg
      661 tctcctggtg ggactaccac gtgtacctgc tggcggggtt cgcggccgcg gcctggtccg
      721 tgacgacgca gtcgcgacaa acccggagcg tgcgcgaggc cttggccacg gtctcgatca
      781 gggacccgat ccagctcgtc tcccggggcc accccgaggc cctggatgcg ctggtcggcg
      841 cggtggaggc gaaggacagc cacatgcgag gccactccgc gcgcgtggcc gaggtcgcga
      901 tgcggatcgg gctccgtatg gacatgggcg ccgacgagct ccgcgggctg gcccagggag
      961 cgttcctcca cgacatcggg aagatcggcg tgccggacga cgtgctgcac aagcgggacc
     1021 ggctgacccg cgaggactgg gcctggatcg agcagcaccc cgtcatcggg ggtgagatgg
     1081 ccggccgcgc gccctctctc cacgattcgc tcgccgtgat ccgccaccac cacgagcggt
     1141 gggacggcag cggctacccc gaccggctgg ccggggacga gatcccgatc gccgctcgca
     1201 tcgccacggt cgcggacgtc tgggacgcgc tcacgtccga tcgctcctac cggccggcgt
     1261 ggccggtgga ccgggcgctg gcgtacatcg ccggcgccgc aggccacctc ttcgaccccg
     1321 tcgcggttga cgcgttcctc gacctcctcg cccatcgggg gctctggccc gaccgcctcc
     1381 agcccgacgt ggacaccctg gcctcgatgg cggaagcgtg tcacccacgg accaacgggc
     1441 acgccgcggg cgtgacggcg gccgagcccg gacgctcggc ctaggccccc gccgtctctg
     1501 cggcctgctc caccacgtcg aactccccca tcacgggctc cgaacccacg gcccggaaca
     1561 tccggatcgc ctcggggacc tccacggcgg cgcggtaggc ctccagctcc tcacgactcc
     1621 tccagatggt gaggatccgc cactcgtgac tctccgggac gtgcaggaga aaggtctgga
     1681 cgatgcccgg aggcaggtcc gcggtcgcgc ccgcgaacga atgccggaac tccgagccgc
     1741 gctcctcggc gacgtgcgcg gcgaccacgg tgaccaccat ggctccccct cttccgacgg
     1801 caccacgccg actctgcgcc ggaacgagca gagggtcaag gacccggggc ttcctccctt
     1861 tccatagggg gccattcgac cctgacccct tcggctggca ctatctaggt tgcctgcaaa
     1921 cggaagggaa gggagtgacc atgacgaggc tctttcggtt cgggggctac ttcgccagca
     1981 tcgtcctgat cctgttcggg atcggggccg cggtgctcgg gaccctgggc ttcctcgagg
     2041 tgcgggacac gatcgcccag gagagcatca ccgccacgcc cgacgcggcc gagatcgagg
     2101 gggtgaacct cgagccgggc caggagatca ggacgggagg cgaggcgaag gagttcgccg
     2161 agatcatcag gtcccacgcg ctggaggcga ccggcggccg ggcgttcgcc gagatccccc
     2221 gctacatcac ggagacgggt gaagagacgg acgacgaggc cgcggccgcg accacggagg
     2281 aggggcggcc ggtcgagaac cccgtccgcg gcatctggaa cacggccacc gcgttcacga
     2341 cggcgctgaa caccgcctac ctcgcggagc agatcgcgct gttcacgatc gcgatgggcg
     2401 tcgctctggt gctggtgggt atcgggttcc tcgtgctgac gatcgcggcg ctgaggggcc
     2461 cggtgttcga gcggcaccgg gacacgacct gacccttccc tggggcggcg cgcgcggcga
     2521 agccgcgcgc g
//
LOCUS       DASUTZ010000225         3318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_205833, whole genome shotgun sequence.
ACCESSION   DASUTZ010000225 DASUTZ010000000
VERSION     DASUTZ010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205833"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..2146
                     /gene="infB"
                     /locus_tag="VF097_09805"
     CDS             2..2146
                     /gene="infB"
                     /locus_tag="VF097_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX6263127.1"
                     /translation="MRIHELAKELDVKSKELIGVLEEMGYSGLTASSSVPDEAVPRLR
                     ASGGKAVPAGKPKVVTEEPVTPTRRKASAKKAKTAKATTTKAEPAGQKAEAAPAQAEA
                     PAEPRAEPAPAEPAAAPEPAAPPAPEPEVEGVKIARGSSVKEVADKLGTEPTSLIRKL
                     LELGQMVTITQSLPDEAIELVAQDLGQPVEIVAPEEDLEPEDDDLDPERMTLRPPVIT
                     VMGHVDHGKTLLLDAIRQTDVVSGEAGGITQAIGAYQVRQDGSEITFIDTPGHEAFTA
                     MRARGAQVTDIAVLVVAADDGVMPQTIEAIDHAAAAAVPIVVAVNKIDKPEADPMRVR
                     QQLAEVGIQPSEWGGDYEFVDVSAKSGEGIEKLLETLLLVSELQELKADPEAAPRGIA
                     IEAHLDKGRGPVATVLVQQGTLRVGDAIVSGAAHGRVRAMFDEHAVPVEEAGPGKPVQ
                     ALGWSSPPEAGDEFRVAEDEREARRIAQEREARQRAAEIVAKRPAQTLEEILATAARE
                     GEIADLNLVLKADTRGSLEALADALAKLSGDEVRVRILHQSVGAIAESDVMLAQASSA
                     VVVGFNVVPDPNARAAAEQEGVDIRTYRVIYEAVDEIKQAMSGLLTPGEEEVALGRAE
                     VRAIFQVPRAGRAAGCYVTQGTITRNARARLVRDGVVVYDGRIASLRRFKDDVREVQE
                     GFECGIGLEDFQDVKEGDVIEAYEVREVARSL"
     gene            2158..2451
                     /locus_tag="VF097_09810"
     CDS             2158..2451
                     /locus_tag="VF097_09810"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEX6263128.1"
                     /translation="MLVALTAFDLRIPLAGSLKEKRRVVKSLTAGLRQRFNVAVAEVD
                     HQDLWRRATLAVSAVGGEGYQIKKVMHEVERYLDRQPEIEVISSHTSLHGHDD"
     gene            2444..2794
                     /gene="rbfA"
                     /locus_tag="VF097_09815"
     CDS             2444..2794
                     /gene="rbfA"
                     /locus_tag="VF097_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX6263129.1"
                     /translation="MTDGGRPERVAEEFRKVLAEEVQRLKDPRVGFITITGVDVTHDL
                     RRAVVYYTVLGEERDRRATAAGLRSAAPRLRAELGRQIRLKFLPELRFEEDQAVAEGD
                     RVDELLRRIHEEET"
     gene            2791..>3318
                     /locus_tag="VF097_09820"
     CDS             2791..>3318
                     /locus_tag="VF097_09820"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEX6263130.1"
                     /translation="MTLDETTLARAARVLEEAEDVALVCHVNPDPDAMGSMLGLAGFL
                     ARRGKKVAATWPNDPAERPRWVDALGNAELIVTPKEMPKEPSVLVTLDAAAANRLNGM
                     IHLLERANVSICIDHHRSNDGFGTINLIDPDASSTAEIVFRLIERMGGGMSDEDAKSL
                     YAGVVTDTGRFQYEAT"
BASE COUNT          549 a         1102 c         1260 g          407 t
ORIGIN      
        1 agtgaggatc cacgagctgg cgaaggagct ggacgtcaag agcaaggagc tcatcggcgt
       61 cctcgaggag atggggtact cgggcctgac ggcctcctcc agcgtgcccg acgaggccgt
      121 cccccgcctg cgggcctccg gagggaaggc ggtccccgcg gggaagccca aggtcgtcac
      181 cgaggagccc gtcacgccca cccggaggaa ggcttcggcc aagaaggcca agaccgcgaa
      241 ggccaccacc acgaaggccg agcccgccgg gcagaaggcc gaggccgccc ccgcccaggc
      301 ggaggcgccg gcggagccgc gggccgagcc cgctcccgcc gagccggccg ccgcgcccga
      361 gcccgcggct ccgccggcgc cggagcccga ggtcgagggg gtcaagatag cccggggctc
      421 cagcgtgaag gaggtcgcgg acaagctggg gaccgagccc acgtcgctca tccggaagct
      481 cctggagctc ggccagatgg tgacgatcac gcagtcgctc ccggacgagg cgatcgagct
      541 cgtcgcgcag gatctgggtc agcccgtgga gatcgtggcc cccgaggagg acctcgagcc
      601 cgaggacgac gacctcgatc ccgagcgcat gaccctcagg ccccccgtga tcaccgtcat
      661 gggccacgtc gaccacggga agaccctttt gctcgacgcg atccgccaga ccgacgtggt
      721 ctcgggcgag gcgggcggca tcacccaggc gatcggcgcc taccaggtcc ggcaggacgg
      781 cagcgagatc acgttcatcg acacgccggg ccacgaggcc ttcaccgcga tgcgagcccg
      841 gggggcccag gtcaccgaca tcgccgtcct ggtcgtggcg gccgacgacg gcgtcatgcc
      901 ccagacgatc gaggcgatcg accacgcggc ggcggccgcg gtcccgatcg tcgtggccgt
      961 gaacaagatc gacaagcccg aggccgaccc gatgcgcgtc cgacagcagc tcgccgaggt
     1021 cggcatccag ccctcggaat ggggcggtga ctacgagttc gtggacgtct ccgccaagag
     1081 cggcgagggg atcgagaagc tgctcgagac gctcctcctc gtctcggagc tccaggagct
     1141 gaaggccgac ccggaggcgg cgcccagggg gatcgcgatc gaggcccacc tggacaaggg
     1201 gcggggaccg gttgcgaccg tgctggtgca gcaggggacc ctgcgggtcg gggacgcgat
     1261 cgtctccggg gccgcccatg ggcgcgtgcg ggcgatgttc gacgagcacg ccgtccccgt
     1321 ggaggaggcc gggccgggga agccggtcca ggcgctcggc tggtcgtccc cccccgaggc
     1381 gggcgacgag ttccgggtgg ccgaggacga gcgcgaggcg aggcggatcg cccaggagcg
     1441 ggaggcccgc cagcgcgcgg ccgagatcgt ggccaagcgg ccggcccaga ccctcgagga
     1501 gatcctggcg acggcggcgc gggagggcga gatcgcggac ctcaacctcg tgctgaaggc
     1561 ggacacgcgc ggctcgctgg aggcgctggc cgatgccctc gcgaaactct ccggcgacga
     1621 ggtccgcgtg cggatcctcc accagagcgt cggggccatc gccgagagcg acgtcatgct
     1681 ggcccaggct tcgagcgccg tcgtagtggg gttcaacgtc gtgcccgacc cgaacgcgcg
     1741 cgcggcggcc gagcaggagg gcgtggacat ccgcacctac cgggtgatct acgaggccgt
     1801 ggacgagatc aagcaggcga tgtcgggcct gctaaccccg ggcgaggagg aggtggcgct
     1861 gggccgggcc gaggtccggg cgatcttcca ggtgccgcga gccggccggg cagccggctg
     1921 ctacgtcacc cagggcacga tcacgaggaa cgctcgggcc cgcctcgtcc gggacggtgt
     1981 cgtcgtctac gacggacgga tcgcgagctt gcgccgcttc aaagacgacg tccgcgaggt
     2041 ccaggagggg ttcgagtgcg gcatcggcct cgaggacttc caggacgtga aggaggggga
     2101 cgtcatcgag gcctacgagg tccgcgaggt cgcgcgctcc ctctagatgt ggtcgcgatg
     2161 ctcgtggcgc tcacggcctt cgatctccgg atccccctcg ccggctcgct caaggagaag
     2221 cgacgggtgg tgaagtccct cacggccggc cttcgccagc ggttcaacgt ggcggtggcc
     2281 gaggtcgacc accaggacct gtggcggcgg gcgacgctcg ccgtgtcggc ggtgggcggg
     2341 gaggggtacc agatcaagaa ggtgatgcac gaagtggaac gatacctgga ccgtcagccg
     2401 gagatcgagg tgatctcgag ccatacgagc ctccacggcc acgatgactg acggcggccg
     2461 ccccgagcgc gtggccgagg agttccggaa ggtcctggcc gaggaggtcc agcggctgaa
     2521 ggatccgagg gtggggttca tcacgatcac cggcgtggac gtcacgcacg atctccgccg
     2581 ggcggtcgtt tactacaccg tgctggggga ggagcgggac cggagagcga cggccgcggg
     2641 actccgctcg gcggccccac ggctgcgcgc ggagctggga cggcagatcc ggctgaagtt
     2701 cctcccggag ctgcggttcg aggaggatca ggccgtcgct gagggtgacc gcgtcgacga
     2761 gctgttgcgg aggatccacg aggaggagac gtgacgctgg acgagacgac gctggctcgg
     2821 gccgcccggg tcctcgaaga ggccgaggac gtggcgctcg tgtgtcacgt caaccccgac
     2881 cccgacgcga tgggctcgat gctcgggctg gccggcttcc tggcgcggcg agggaagaag
     2941 gtggccgcga cctggcccaa cgacccggcc gaacggcctc gatgggtgga cgcgctgggg
     3001 aacgccgagc tcatcgtcac ccccaaggag atgccgaagg agccctcagt cctggtgacc
     3061 ctggacgccg ccgccgccaa ccggctgaac gggatgatcc acctcctgga gcgggcgaac
     3121 gtgtcgatct gcatcgacca tcaccgctcg aacgacggct tcgggacgat caacctgatc
     3181 gatcccgacg cctcctcgac ggcggagatc gtcttccggc tgatcgagcg catgggaggc
     3241 gggatgtcgg acgaggacgc gaagtcgctg tacgcgggcg tcgtgaccga caccgggcgg
     3301 ttccagtacg aggcgacc
//
LOCUS       DASUTZ010000226         4909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1633368, whole genome shotgun sequence.
ACCESSION   DASUTZ010000226 DASUTZ010000000
VERSION     DASUTZ010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4909
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633368"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2125
                     /locus_tag="VF097_09825"
     CDS             <1..2125
                     /locus_tag="VF097_09825"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX6263131.1"
                     /translation="GSAVTVVLKRVENPLEFGIVIADDEGRIERFLEKPGWGEVFSDT
                     INTGIYVVEPEVLNHVPAGEEYDFAGQLFPLLLDKGLPVYGFVAEGFWTDVGNLEAYL
                     QVHRDILDEKVRVDIEGYRTEAGVWRGEGSEVDPDAQIHGPVLIGENARIEAGASVHE
                     YSVLGKGVVVKSGAFVHRAIVHDGAYIGSSAAIRGCVVGKNVDVKHGARLEEEVVVAD
                     EVYVGEGAVLSPRVKVFPFKRIDPGALVSKSIVWETTTQQGLFGERGVAGLVNIDITP
                     EMAMRLALAFASTMPKKSVLTAGRDPTRTARVIKRAMVAGVNAAGVNAHDLELVPTPV
                     TRFYARTSRAEGGFVVRTAPGDAQSVEIVFIDERGIDIGPSAQRKVERGFFRDDLRRA
                     FHHEIGELTFPARGREYYLDGILGVVDAETVREARPKVVIDYGWGAAAITAPLVLGRL
                     GADTLAINATVEEERSVLNRQQQEQHLEALGGLVRAGGADLGVLMDSTGERMWLVDER
                     GRVVGLSQALLTFVRLVADTVPAPTIAVPVASSRVVEEIVGERGGKVVWTGISPAALS
                     AASDAGDVAFAGAEGGGYIFPSFLPAYDAVMSLSKLLELLIRSGRSLGEVVDGLPSTH
                     VVRVDVPCPWEAKGTVMRRLVERFAEEDVVTIDGVKRFRGRDWALIVPHPLEPVVRVW
                     AEADEEEAARALAADHASLVEELRG"
     gene            2157..2885
                     /locus_tag="VF097_09830"
     CDS             2157..2885
                     /locus_tag="VF097_09830"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEX6263132.1"
                     /translation="MREARVHIGLWIVLALFGFLVSVGFLQERNREETSPARRAELER
                     LIEQRRGAIHELATEVSRLSSRVDDVRKRAGAGSSEVDDLVRRLEGLRREVGLEGVEG
                     PGVVVELSDSPKPATTRDELTDLRIQDVDLQLVANALWRSGAEAVSVNGRRLTATSAI
                     RKAGSVILVNYRAVSSPYRIAAIGNPEALLDGLEDSEIAERFAVWKEIYGLGFSIRSE
                     DQLHAPALPGLQEFRHAVPVKADR"
     gene            2882..3211
                     /locus_tag="VF097_09835"
     CDS             2882..3211
                     /locus_tag="VF097_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013476763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HEX6263133.1"
                     /translation="MIALIALLAGIAIGLFFQPTLPAALAPFLPVIVVAALDTVLAGL
                     LSRLEGGYREGEFIVAFLSNTLLAAFIVWVGDRLGAPDLTIGVVVVFGIRMFQSLAGI
                     RHYLFRD"
     gene            3211..3948
                     /locus_tag="VF097_09840"
     CDS             3211..3948
                     /locus_tag="VF097_09840"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEX6263134.1"
                     /translation="MDYLDLHDPGPRRPTMRPPSRTQTKIALVVVALAVGLLVGVQAG
                     TRGDATARLAAEAPEDLARILSDLNEEADQLARQLADLRLKLFRYEGTAERGDLALRD
                     ARKTLRDLQVLAGTTEVRGPGLRVDLADQMARVSWEPMLDLVQELRDAGAEAIAVSGV
                     RVVASSWFAPGDAGMTIDGEHLVPPYRIEAIGDPPALREALGIPGGPISLIEAQPGVS
                     ISVQELDRMRLPALRREITFRYARPAS"
     gene            3961..4359
                     /gene="gcvH"
                     /locus_tag="VF097_09845"
     CDS             3961..4359
                     /gene="gcvH"
                     /locus_tag="VF097_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008341044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX6263135.1"
                     /translation="MDYPENLRYTKEHEWAAAEDANRVRVGITDFAQDALGDVVYVDL
                     PEEATEVRAGEPFGEVESTKSVSDIFSPVSGRVAERNAALEDQPELVNEDPYGEGWMV
                     VVEMSDPAELEELLTAEGYRSFAEGGDEEA"
     gene            4448..4903
                     /locus_tag="VF097_09850"
     CDS             4448..4903
                     /locus_tag="VF097_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006540372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX6263136.1"
                     /translation="MYCTRCGERNPDDARFCAHCGHALQDDTTVGLAATEIEDEAGEE
                     IYLPLDELQAGQALLVVKRGPTAGSRILVDKDVTTAGRHPESDIFLDDITVSRRHAEF
                     RRKEGRFFIHDMGSLNGTYVNRDRVDSTQLASGDEVQIGKFKLVFFMGE"
BASE COUNT          716 a         1592 c         1883 g          718 t
ORIGIN      
        1 gggctcggcc gtgaccgtgg tgctgaagcg cgtggagaac cccctggagt tcgggatcgt
       61 gatcgccgac gacgagggcc ggatcgagcg cttcctggag aagccgggct ggggcgaggt
      121 cttcagcgac acgatcaaca ccgggatcta cgtcgtcgag cccgaggtcc tgaaccacgt
      181 gcctgcgggc gaggagtacg acttcgccgg ccagctcttc ccgctgctgc tggacaaggg
      241 cctgcccgtg tacgggttcg tggccgaggg cttctggacc gacgtgggga acctcgaggc
      301 gtacctccag gtgcaccggg acatcctcga cgagaaggtg cgggtggaca tcgagggcta
      361 ccgcacggag gccggcgtgt ggcggggcga gggatccgag gtcgatcccg acgcgcagat
      421 ccacggaccc gtcctcatcg gcgagaacgc gcggatcgaa gccggtgcct cggtccacga
      481 gtactcggtg ctgggcaagg gcgtcgtggt gaagagcgga gcgttcgtcc accgggccat
      541 cgtccacgac ggcgcctaca tcgggtcctc ggccgcgatc cggggctgcg tggtgggcaa
      601 gaacgtggac gtgaaacacg gcgcccgcct cgaggaggag gtcgtcgtgg ccgacgaggt
      661 gtacgtcggc gagggggccg tcctcagccc gagggtgaag gtcttcccgt tcaagcggat
      721 cgaccccggg gcgctcgtct ccaagagcat cgtgtgggag accaccacac agcagggcct
      781 gttcggggag cggggcgtgg ccgggctcgt caacatcgac atcacccccg agatggcgat
      841 gcgcctggcc ctggccttcg ccagcacgat gccgaagaag tcggtcctga cggcagggag
      901 ggaccctaca cggaccgcgc gggtgatcaa gcgggctatg gtcgcgggcg tgaacgcggc
      961 cggcgtcaac gcgcacgacc tggagttggt cccgaccccg gtgacgcgct tctacgccag
     1021 gaccagccgc gccgaaggcg ggttcgtggt ccgcacggcc ccgggcgatg cccagtcggt
     1081 ggagatcgtc ttcatcgacg agcgggggat cgacatcggc ccgtccgccc agcggaaggt
     1141 ggagcgaggg ttcttccggg acgacctccg ccgggccttc caccacgaga tcggggagct
     1201 caccttcccg gcgcggggtc gcgagtacta cctggacggg atcctgggcg tggtcgacgc
     1261 ggagacggtc cgggaggccc ggcccaaggt cgtgatcgac tacggatggg gcgccgcggc
     1321 gatcaccgct cctctggtgc tggggaggct gggcgcggac accctggcca tcaacgccac
     1381 ggtcgaggag gagcgctcgg tcctcaaccg tcagcagcag gagcagcacc tggaggcgct
     1441 gggggggctc gtccgggcgg gtggggccga cctcggcgtc ctgatggatt ccaccggcga
     1501 aaggatgtgg ctcgtggacg agcgcgggcg cgtcgtcggg ctgagccagg ccctgctgac
     1561 gttcgtacgc ctcgtggccg ataccgtccc cgcgcccacg atcgccgtgc cggtcgcctc
     1621 gagccgggtc gtggaggaga tcgtgggcga gcgcgggggc aaggtcgtct ggaccgggat
     1681 ctcgccggcc gcgctctcgg cggcgagcga cgcgggcgac gtcgccttcg ccggcgccga
     1741 gggcggcggc tacatcttcc ccagcttcct cccggcctac gatgccgtca tgagcctgtc
     1801 gaagctgctc gagctcctga tccggagcgg ccgttccctg ggggaggtcg tggacggcct
     1861 ccccagcacc cacgtcgtgc gcgtcgacgt cccctgcccg tgggaggcga aggggaccgt
     1921 gatgcggcgg ctcgtcgagc ggttcgccga ggaggacgtc gtgacgatcg acggcgtgaa
     1981 gcggttccgg gggcgcgact gggcgctgat cgtgccgcac cccctcgagc cggtcgtgcg
     2041 ggtgtgggcg gaggccgacg aggaggaggc ggcccgggcg ctcgccgccg accacgcctc
     2101 gctggtggag gagctgcgag ggtagcgtcg ggcggggcgc ggctggaggg cgtgcgatga
     2161 gggaggcgcg ggtccacatc ggtctctgga tcgttctggc cctcttcggg ttcctcgtga
     2221 gcgttgggtt cctccaggag cggaaccgcg aggagacctc gccggcccgc cgagccgagc
     2281 tggagcgcct catcgagcag cgccgggggg cgatccacga gctcgccacg gaggtgagca
     2341 ggctctcgtc gcgggtggac gacgtccgga agcgagcggg ggcgggatcc tcggaggtcg
     2401 acgacctcgt ccggcggctc gagggcctgc gccgggaggt ggggctggag ggcgtcgagg
     2461 gccccggcgt cgtggtggag ctctccgaca gccccaagcc ggccacgacg cgcgacgagc
     2521 tgaccgacct caggatccag gacgtggatc tccagctggt ggcgaacgcg ctgtggcgat
     2581 cgggggccga ggccgtctcg gtgaacgggc ggcgcctcac cgcgacctcg gcgatccgca
     2641 aggccgggag cgtgatcctc gtgaactacc gggcggtgtc gtcgccctac cggatcgccg
     2701 cgatcgggaa cccggaggcg ctgctcgacg ggctggagga ctcggagatc gccgagcggt
     2761 tcgcggtgtg gaaggagatc tacgggctgg gcttctcgat ccggtccgag gaccagctcc
     2821 acgcccccgc gctcccgggc ctccaggagt tccgccacgc ggtgcccgta aaggcggatc
     2881 gatgatcgcg ctgatcgccc tgctggcggg gattgcgatc gggctgttct tccagcccac
     2941 cctgccggcc gcgctggctc ccttccttcc ggtcatcgtg gtggccgccc tcgacacggt
     3001 gctggccggc ctcctgtccc gcctcgaggg aggataccgg gagggggagt tcatcgtggc
     3061 gttcctctcg aacaccctgc tggccgcgtt catcgtctgg gtgggggaca ggctgggagc
     3121 tcctgacctg acgatcggcg tggtggtcgt gttcgggatc cggatgttcc agagcctggc
     3181 ggggatccgc cactacctgt tccgagactg atggactacc tcgacctcca cgatccgggc
     3241 ccgaggcggc cgacgatgcg gccgccgagc cgcacgcaga cgaagatcgc gctcgtggtc
     3301 gtcgccctcg ccgtgggcct gctggtcggg gtccaggcgg gcacccgagg ggacgccacg
     3361 gcccggctcg ccgccgaggc gcccgaggac ctggcccgca tcctctccga cctgaacgag
     3421 gaggccgacc agctcgcgcg ccagctggcc gacctgcggc tcaagctgtt ccgctacgag
     3481 gggacggccg agcgcgggga cctcgcgctg cgcgacgcgc ggaagacgct ccgggacctc
     3541 caggtcctgg ccgggacgac cgaggtgcgc gggccgggcc tccgggtcga cctcgcggat
     3601 cagatggccc gggtgtcgtg ggagccgatg ctcgatctcg tccaggagct tcgcgacgcg
     3661 ggggccgagg ccatcgccgt gagcggtgtg cgcgtggtcg cctcgagctg gttcgccccc
     3721 ggagacgccg ggatgaccat cgatggggag catctcgtgc cgccgtaccg gatcgaggcg
     3781 atcggcgacc cgccggcgct gcgggaggcc ctggggatcc cgggaggccc gatctcgctg
     3841 atcgaggccc agccgggagt gtcgatctca gtgcaggagc tcgaccgtat gcgcctgccg
     3901 gcgttgcgtc gagagatcac gttccgatac gctcgcccgg cctcgtgacg gagggggacc
     3961 atggactacc cagagaacct gcgctacacg aaggagcacg agtgggcggc ggccgaggac
     4021 gcgaacaggg tccgggtcgg catcacggac ttcgcgcagg acgccctggg cgacgtcgtc
     4081 tacgtggacc tgcccgagga ggcgaccgag gtcagggccg gcgagccctt cggcgaggtc
     4141 gagtcgacga agtcggtctc ggacatcttc agccccgtgt cgggccgcgt ggccgaacgc
     4201 aacgcggcct tggaggacca gcccgagctg gtgaacgagg atccctacgg cgaggggtgg
     4261 atggtcgtgg tcgagatgtc cgatccggcc gagctcgagg agctgctcac cgccgagggg
     4321 taccggtcgt tcgccgaggg aggagacgag gaggcctgag cccgtccagc cctcgttgac
     4381 cgttctggga ccgtccgata cagtcgcctc agcctctccc tgaggcttcg ggtcggatcg
     4441 gaggccgatg tactgcaccc gctgcggtga gaggaaccca gacgacgcgc gcttctgtgc
     4501 gcactgcggt cacgccctcc aggacgacac gacggtgggt ctggccgcga ccgagatcga
     4561 ggatgaagcg ggggaggaga tctacctgcc tttggacgag ctgcaggccg ggcaggcgct
     4621 cctcgtggtg aagcggggtc cgaccgcggg ctcgcgcatc ctcgtggaca aggacgtgac
     4681 gacggccggg cgccaccccg agagcgacat cttcctcgac gacatcacgg tgtctcggcg
     4741 gcacgcggag ttccgccgca aggagggcag gttcttcatc cacgacatgg ggagcctcaa
     4801 cggcacgtac gtcaaccgcg accgggtgga cagcacgcag ctcgccagcg gggacgaggt
     4861 gcagatcggc aagttcaagc tcgtgttctt catgggggag tgatggcgg
//
LOCUS       DASUTZ010000227         3476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1633697, whole genome shotgun sequence.
ACCESSION   DASUTZ010000227 DASUTZ010000000
VERSION     DASUTZ010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633697"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VF097_09855"
     CDS             <1..869
                     /locus_tag="VF097_09855"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX6263137.1"
                     /translation="DELRTEGRRIPSYSYPEDAARALAHAYRHAEWRGRPEGTPPRIE
                     ARNDEAAALLGEIVAAGPRWLEPDETFRLLDCYGIPLASWRLASTPEEAGAAAGELGG
                     EVALKAVAPGLIHKTEAGAVRLSLEGSDAVRGAAEEMAASVKQAGHEVEGFLVQAMVS
                     GGIEMLVGVVHDALFGPVVACGAGGTTAELVKDVAVRITPLTDLDASEMIRSLQTFPL
                     LEGYRGAPGGDVAALEDLLLRVGAMVEDNPAIAEMDLNPVIVLPEGLAVVDARVRAEI
                     PAPSRPLSARRR"
     gene            957..1154
                     /locus_tag="VF097_09860"
     CDS             957..1154
                     /locus_tag="VF097_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263138.1"
                     /translation="MAEFQLAGRLVQGTRRPSRRYPRGRVCVHDGCETVLSVYNPARF
                     CAQHEATQLPRTFGRQRLRSA"
     gene            1177..1953
                     /gene="otsB"
                     /locus_tag="VF097_09865"
     CDS             1177..1953
                     /gene="otsB"
                     /locus_tag="VF097_09865"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX6263139.1"
                     /translation="MSEGLPIRDLPDARERLADLAGLSPAIFLDYDGVLTPIVQAPEQ
                     AILGDEMRGALLRLRDRWPLAVVSGRDLQDVRERVGVDGVAYAGSHGFEIVTPGGRHE
                     LKGEEYLPDLDAAEGELREALPGGARLERKRVAIAVHVRGMEADGIPGVGRVVDEVAA
                     RHPRLRVTGGKRILELRPDLDWDKGRALRWFLEVLGLTDAVPVYLGDDVTDEDAFREI
                     RDSGLAVVVRGEDDERPTLAHAALDGPDDVRSFLEALADL"
     gene            complement(1956..2717)
                     /locus_tag="VF097_09870"
     CDS             complement(1956..2717)
                     /locus_tag="VF097_09870"
                     /inference="COORDINATES: protein motif:HMM:NF040399.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6390 family protein"
                     /protein_id="HEX6263140.1"
                     /translation="MTLSGSLLFARYAFPPNALGYCGPDASPVMAEHLAQGHADPGLE
                     GLARGFEGAWPYLRLIAESNGIGDPLEDRVVEAYWVGGPLLDRVRAGALGRFLEETFR
                     SRAGTSWRVLEGLAGAGGVPHHNLHVFGVYPWLGLLRMGHEAEPMRVLEACRTRWARV
                     LAVEPGRALVRSRTLAWDGHALAFGHPTVSWAATEAGGREVAPRLAPGDLVSVHWDRV
                     CDRLTRRGARTLHRETLRALAAAQRSSVGPAAVLS"
     gene            complement(2714..>3476)
                     /locus_tag="VF097_09875"
     CDS             complement(2714..>3476)
                     /locus_tag="VF097_09875"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6263141.1"
                     /translation="IAAATTFPRREPGARPTGRRPTATLLALGLVGGSIPFVLFFEGL
                     ARASSVQAAFIHKTLLLWVAALAVPLLRERVGLPHLGAFALLIAGQAALTGGLSGFGW
                     GAGEWMVLVATLLWAVELVIAKRLLRSFGSSTLGIARMGVGSVALLGWLAVTGRLGLL
                     LGLEASQWAWALITGAILAAFVGTWFAALARAQAVDVTAVLVFGAVVTGVLAAGVQGV
                     PLASQAPGLLLVTLGTAIVAVAALRPVPARAREVG"
BASE COUNT          479 a         1273 c         1321 g          403 t
ORIGIN      
        1 cggacgagct gcggaccgag ggtcggcgga tcccctcgta ctcgtatccg gaggacgcgg
       61 ctcgcgccct ggcccacgcg taccggcacg cggagtggcg cgggcggccc gaggggacgc
      121 cccccaggat cgaggctcgg aacgacgagg cggcggcgct cctcggcgag atcgtggccg
      181 ccggcccgcg ctggctcgag cccgatgaga ccttccgcct cctcgactgc tacgggatcc
      241 cgctggcctc ctggcggctg gcctcgacgc cggaggaggc tggcgcggcg gccggggagc
      301 tcgggggcga agtcgctctc aaggcggtgg ccccggggct gatccacaag accgaggccg
      361 gcgccgtgcg gctctcgctg gagggatcgg atgccgtccg cggcgccgcc gaggagatgg
      421 cggcctccgt gaagcaggcc ggccacgagg tcgaggggtt cctggtccag gcgatggtct
      481 cgggcggcat cgagatgctg gtcggggtgg tccacgacgc gctgttcggg ccggtcgtgg
      541 cgtgtggggc cggggggacc acggccgagc tggtgaagga cgtggccgtc aggatcactc
      601 cgctgacgga cctcgatgcg tccgagatga tccgttcgct ccagaccttc cccctcctcg
      661 agggctaccg cggcgctccc ggcggggacg tcgccgcgct cgaggacctc ctcctccggg
      721 tcggcgccat ggtcgaggac aaccccgcca tcgccgagat ggacctcaac cccgtgatcg
      781 tgctgcccga gggcctggcc gtcgtggacg caagggtccg ggccgagatc cccgccccca
      841 gccgtcccct gtcggcgcgc cgccggtgac agggtccgac ggtccctctc gctgcgggac
      901 cctcggccct cggatggagg cgcccggact gagacccttc gtgccggagg gatcgaatgg
      961 ccgagttcca gctggcgggg aggctcgtac agggcacgcg acgccccagc cgccgctacc
     1021 cgagagggcg ggtgtgcgtg cacgacggct gcgaaaccgt gctctccgtc tacaaccccg
     1081 cgaggttctg cgcccagcac gaggcgacgc agctgccccg cacgttcgga cgccagcgcc
     1141 tgcgctccgc ctgacccccc cataccggcg ggcggcgtga gcgaggggtt gccgatccgc
     1201 gatctgcccg acgccaggga gcgtctcgcc gacctggccg gactgtcccc ggcgatcttc
     1261 ctcgactacg acggggtcct gaccccgatc gtccaggccc ccgagcaggc gatcctgggc
     1321 gacgagatgc ggggcgcgct gctgcgtctg cgggaccgct ggcccctcgc tgtggtcagc
     1381 ggtcgggatc tccaggacgt gcgcgagcgc gtgggcgtgg acggcgtggc ctacgcgggg
     1441 agccacggat tcgagatcgt caccccgggg ggccgtcacg agctgaaggg ggaggagtac
     1501 ctgcccgatc tcgacgcggc cgagggcgag cttcgtgagg cgctcccggg cggggcccga
     1561 ctcgagcgga agcgggtggc gatcgcggtg cacgtccggg ggatggaggc cgacgggatc
     1621 ccgggcgtgg gccgggtcgt ggacgaggtg gccgcccggc acccgcgcct cagggtcacg
     1681 ggcgggaagc ggatcctcga gctccgcccg gatctggact gggacaaggg ccgagccctc
     1741 cgctggttcc tcgaggtgct ggggctgacg gacgccgtcc cggtctacct gggggacgac
     1801 gtcaccgacg aggacgcgtt ccgggagatc cgcgactcgg gcctcgccgt cgtcgtgcgg
     1861 ggcgaggacg acgagcggcc gaccctggcc cacgccgccc tagacggccc cgacgacgtc
     1921 cggagcttcc tggaggcgct ggcggatctc tgagatcagc tcaggacggc cgcggggccg
     1981 acggagctcc gctgagcggc ggcgagcgct cggagggtct cccggtggag ggtccgtgct
     2041 ccccggcgcg tgagtcggtc gcagacccga tcccagtgga ccgagaccag atcgccgggg
     2101 gccaggcgag gcgccacctc tctccctccc gcctccgtcg ccgcccagct cacggtgggg
     2161 tggccgaagg cgagcgcatg gccgtcccac gccagggtcc gtgagcggac cagcgcccgt
     2221 ccgggctcca ccgccagcac ccgggcccac cgggtccggc aggcctcgag gacccgcatg
     2281 ggctcggcct cgtgccccat ccggaggagc cccagccacg ggtagacccc gaacacgtgg
     2341 aggttgtggt gcgggacgcc ccccgcccct gccagcccct cgaggactcg ccacgaggtc
     2401 cccgcccggc tccggaacgt ctcctcgagg aagcggccga gcgcgcccgc cctcacccga
     2461 tcgaggaggg gtccgccgac ccagtaggcc tcgaccacgc ggtcctcgag cgggtccccg
     2521 atcccatttg actcggcgat cagccggagg tagggccagg ccccctcgaa gccgcgggcc
     2581 agcccttcga gacccggatc cgcgtgcccc tgggccaggt gctcggccat cacgggcgat
     2641 gcgtcggggc cgcagtaccc gagcgcgttg ggcgggaagg cgtagcgcgc gaagagcagc
     2701 gagccgctga gggtcacccc acctcccggg ctcgggccgg gaccggccgc agggctgcca
     2761 cggcgacgat cgccgtgccc agggtcacca ggagcaggcc gggagcctgg gaggcgagcg
     2821 ggacgccctg cacgcccgcg gccagcacgc cggtgacgac cgcgccgaag acgaggacgg
     2881 cggtcacgtc gacggcctgg gcccgggcca gcgccgcgaa ccaggtcccc acgaaggccg
     2941 ccaggatggc gccggtgatc agggcccacg cccactggct cgcttcgagc cccagcagga
     3001 ggcctagacg ccccgtcacg gcgagccagc cgagcagcgc cacggagccc acgcccatcc
     3061 gcgcgatgcc cagggtggag gacccgaagg agcggagcag gcgcttggcg atcacgagct
     3121 cgacggccca caggagcgtg gccacgagca ccatccactc gcccgcgccc cacccgaagc
     3181 cgctcaggcc gcctgtgagc gcggcctggc ccgcgatcag gagagcgaac gcccccaggt
     3241 gcggcagacc cacccgctcg cggagcagcg ggaccgccag ggccgcgacc cacagcagca
     3301 gggtcttgtg gatgaaggcc gcctggacgg acgaggcgcg ggccaggccc tcgaagaaga
     3361 gcacgaaggg gatgctgccg ccgaccagcc ccagcgcgag gagggtcgcc gtcggtcggc
     3421 ggcccgtcgg ccttgcgccg ggctcgcggc gcgggaacgt cgtcgcggcg gcgatc
//
LOCUS       DASUTZ010000228         7527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1374067, whole genome shotgun sequence.
ACCESSION   DASUTZ010000228 DASUTZ010000000
VERSION     DASUTZ010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7527
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374067"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1337)
                     /locus_tag="VF097_09880"
     CDS             complement(<1..1337)
                     /locus_tag="VF097_09880"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEX6263142.1"
                     /translation="MRVDAQLLSEVRDRFEHAVLVFLDPEGYPMSVATAYSVDLQRGV
                     VELDRPAGTLPAPGTEVNVIFSHIRPQPEMGYDERRYVQLWGRLDEAGDRLRLAPERA
                     HSWDEQKVPFFQYSELTVPQAHRYMEELSERQGRKVRPRLSFGWLALRTTRAPFLSAT
                     IVPVLLGIAVAALQGSWDLWLALLTVLAASFIHLALNVANDVFDTMSGADAANTTPTK
                     FSGGSRVAHYGLVSLKGLALLSAGLYAAGIGIGLYLALVTDFWPLFWLGVAGVAISVF
                     YTAPPFRLVHRGLGEIAVGLGFGPIMVLGAYFVQAERFSWEAGFASIPVAILISLILY
                     VNEIPDRRGDAAAGKRTLPVRWSKGAVIAAYVVAATLTYLAIAGGALVGIIPRPTILA
                     LATIPLAVKVARGLWEHYDSPYTLMGVMGVNIQLHALTGGLLILGYLIAIWAD"
     gene            complement(1334..1822)
                     /locus_tag="VF097_09885"
     CDS             complement(1334..1822)
                     /locus_tag="VF097_09885"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX6263143.1"
                     /translation="MGALPEPIEEVLNAALVCELSVVSAAGRPVTHPMIPLYDGERIY
                     MTSSALFSRKLDHIRANPKVSISISDLGATHDEPMGRRVTVQGEARILEDDPHTTWER
                     ILPLWIAKEPIVKGFYEQRVALPLFWERALIEVTPRRVLVWEDGDTSRDPEVVELEEA
                     AR"
     gene            2079..2678
                     /gene="rpsD"
                     /locus_tag="VF097_09890"
     CDS             2079..2678
                     /gene="rpsD"
                     /locus_tag="VF097_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX6263144.1"
                     /translation="MLKRGPRHRICRRLGVAVCGRPNCPVEKRPYPPGEHGRARRRET
                     EYQVRLAEKQKLRAIYGIGERQFRKYFAKASRRHGVTGEALFQMLETRLDSLVLRLGL
                     ALSQRQARQLVSHGHVLVDGRRVSIPSAQVRPGQTVTVADKARNFVSVREALEVAPDV
                     PPYLSRTEAAGGRLERLPERGEIPLPVEVDERLIVEYYA"
     gene            2692..3381
                     /locus_tag="VF097_09895"
     CDS             2692..3381
                     /locus_tag="VF097_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6263145.1"
                     /translation="MSEPILEARKVVKTYHTGAADVQALRGLDLTVHPGEMVAVVGPS
                     GSGKTTLLNCLSGLDEIDEGEVSIAGQEIHSMPDRVRTSFRGREMGFVFQAFNLIQVF
                     TAAENVELPLLLNGVPPREARVQAENVLDLVGLGHRLGHRPPELSGGEQQRVAIARAL
                     AAGPSIVWADEPTGNLDSVTAEQVMTLLRRLNEEGLTVILVTHDLAIARTASRIVSMH
                     DGQIVGDEPLP"
     gene            3378..3524
                     /locus_tag="VF097_09900"
     CDS             3378..3524
                     /locus_tag="VF097_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263146.1"
                     /translation="MRWRRPLKGQACRRRPPWLGLALVAVLLVAATLALPSLLPGLLP
                     GTLP"
     gene            3521..6427
                     /locus_tag="VF097_09905"
     CDS             3521..6427
                     /locus_tag="VF097_09905"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX6263147.1"
                     /translation="MNPTYLPALAILAGLLGLLAIVTAARHAVPRRLALRSIARRPGE
                     TVLVILGSLLGTAIITGSFIVGDTLDSSIRASAETQLGPIDETVTGFGTESLPLLREA
                     LDGLEDEPGVDGVTFGIRAAGTAAVRLGTDDVAVQPRLLVLEMDLEEAGSFGGDPAAT
                     GLDGAVTPAQGEAVITEDLAAHLDVEPGDEITLFAYGSERELRVTGLVPRLGVAGYTT
                     EFSAESFNVFVAPGTLEELVAAAPPDLEASPPTSLAFVSNEGGVFEGEEPTDEVVPLI
                     RERIEGLEGVDVVDSKRDVVDAADRSGSQFSELFLSIGSFAVIAGILLLVNIFVMLSE
                     ERKGELGMMRAVGMRRGQLVRSFYIEGSLYGLVASAIGAVAGIAVGAAIVRVAADIFA
                     GAGEFSLELVLSAPLVSIVGGFLIGLAISLLTALFTSARISRLNIIRAIRDLPEPPAR
                     RHRLRGLVLGAVALMVGGGMTAFAIVEGEAASAFAGPGILALGLAGLLWRLVPRRPLG
                     SVLGLAVLGWGIFGPTLAPEVFGEADIPVFVIQGVLLTAAAVAVLGFNQESVGHAVRA
                     VAGGARNLAVRLGLAYPLAKRFRTVMTLSMYALVVFTLVFISVLSNIFGGQVEDFTQQ
                     EAGGYDLLVRSSAANPLTADAAAAEDGVEAASPIRYAAFSVEFSTELQPEAEPWALSG
                     VEEGFLAQGPPALEEYAPEYGSEGETWAALLADPSLIILDGFFLQQGGPEAPPADLGD
                     AVTIRDPLTGREVERTVIGVTRGGFSFSGPFVSEESILELVGPRAVATRLYVTLEPGP
                     DPVEVATRLEEEHIPSGVEARTFRAIVSESQSVNLQFIRLMQGYLALGLVVGISGLGV
                     IMVRAVRERRREIGILRSLGFQSGTVGRSFLLESAFVALEGLVVGAALAIVTSYQLVE
                     NAEIFGDLDVTFVIPWGEIAIVLAATLVASLLATGWPARQASRIRPAVALRVAD"
     gene            6497..6820
                     /locus_tag="VF097_09910"
     CDS             6497..6820
                     /locus_tag="VF097_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263148.1"
                     /translation="MRRMLVAAIVAAFVVGAAGIAQADHTHVRILPTGDCVVLAADGN
                     EKYIVLPDENPQVDAELPENRKHPLHVLVHLGQPGSDGSIAVMGSAQDPCSEAGGQTG
                     SYVNP"
     gene            6893..>7527
                     /locus_tag="VF097_09915"
     CDS             6893..>7527
                     /locus_tag="VF097_09915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX6263149.1"
                     /translation="MRVCLMVEGQEGVGWDDWLALARACEDHGLEGLFRSDHYLSVED
                     DETRGSLDAWTTLAALAAETERIRLGTLVSPVTFRHPSLLAKAAVTADHVSGGRVELG
                     IGAGWHLREHEAYGFPFPEPPERMDLLAEQIEIVHRQWSEEMVSFQGKHYRLEAVKAL
                     PKPVPDPHPPLIMGGHAKPRSVELSARWADEYNVFFSTPEECRAKRARVEEA"
BASE COUNT         1051 a         2566 c         2782 g         1128 t
ORIGIN      
        1 ctatccgccc agatcgcgat caggtagccg aggatcagga gcccgccggt gagggcgtgg
       61 agctggatgt tcacgcccat cacgcccatc agcgtgtagg gcgagtcgta gtgctcccac
      121 agcccgcggg ccaccttcac cgccagcggg atcgtggcca gggctaggat cgtgggtcga
      181 gggatgatcc cgacgagggc gccgcccgcg atggcgaggt acgtgagggt ggcggcgacg
      241 acgtaggcgg cgatgacggc gcccttggac caccgcacgg gcagggtgcg cttcccggcc
      301 gccgcgtcgc ctctccgatc ggggatctcg ttcacgtaga ggatcagcga gatcaggatc
      361 gccacgggga tcgaggcgaa ccccgcctcc cacgagaagc gctcggcctg aacgaagtag
      421 gcgcccagca ccatgatcgg cccgaacccc aggcccacgg cgatctcgcc cagcccccgg
      481 tgaacgaggc ggaagggcgg cgccgtgtag aagacgctga tcgccacgcc ggccacgccc
      541 agccagaaca gtggccagaa gtcggtgacg agggcgaggt acagcccgat cccgatgccc
      601 gccgcgtaga gacccgccga gagcagggcc agccccttca ggctgacgag gccgtagtgc
      661 gccaccctgg agcctccgct gaacttcgtg ggcgtcgtgt tcgccgcgtc cgcgcccgac
      721 atcgtgtcga acacgtcgtt cgccacgttg agcgcgaggt ggatgaacga ggccgccagc
      781 accgtgagca gcgccagcca caggtcccac gagccctgga gcgccgcgac ggcgatcccc
      841 agcaggaccg ggacgatcgt ggccgagagg aagggggccc gggtcgtgcg cagggcaagc
      901 cacccgaagg agaggcgggg gcgcaccttc cgcccctggc gctccgagag ctcctccatg
      961 tagcggtggg cctgaggcac ggtgagctcg gagtactgga agaacggcac cttctgctcg
     1021 tcccaagagt gcgcgcgctc gggagccagc cggaggcggt ctcccgcctc gtcgagccgt
     1081 ccccagagct ggacgtagcg ccgctcgtca tagcccatct cgggctgggg acggatgtgg
     1141 ctgaagatca cgttgacctc ggtgccgggg gcggggaggg tgccggccgg acggtcgagc
     1201 tccacgaccc cgcgctgaag atccaccgaa taggccgtgg ccacgctcat cggatatccc
     1261 tcggggtcga ggaagacgag cacggcgtgc tcgaagcggt cgcggacctc agacagcagc
     1321 tgcgcgtcga ccctcatcgc gccgcctcct cgagctcgac gacctccggg tcgcgcgagg
     1381 tgtcgccgtc ctcccagacg agcacgcggc gcggcgtgac ctcgatcagg gcccgctccc
     1441 agaacagagg cagcgccacg cgctgttcgt agaagccctt cacgatgggc tccttggcga
     1501 tccacagcgg caggatccgc tcccaggtcg tgtgggggtc gtcctcgagg atcctcgcct
     1561 cgccttggac ggtgacgcgt ctgcccatcg gctcgtcgtg cgtcgcgccc aggtcgctga
     1621 tcgagatcga gaccttgggg ttggcgcgga tgtggtcgag cttgcggctg aacagcgcgc
     1681 tcgaagtcat gtagatgcgc tcgccgtcgt agagggggat catcgggtgg gtcaccgggc
     1741 ggcccgcggc gctcaccacc gagagctcgc agacgagggc cgcgttcagc acctcctcga
     1801 tcggctcggg cagggctccc actgggtcgt cctcctctgg tcggatgtcc gcggagacag
     1861 agtagccgag cgcgtcaccc agcgcgggcg ccgggcgccg gatggtaggt gagcaggacg
     1921 tgcccggagg ggaaggtgct gcatccgcca ggggaacgcg aacagccgtc tcatccgcag
     1981 cttcttccgt cgatgcgctc acgaagaccc ttctgggagc ggcggctcat cggccgtagt
     2041 cgcggagcgg gctcctgagg tagcatccac ttcctgccat gctgaagcga ggaccacgac
     2101 accgcatctg ccgccgcctg ggcgtcgccg tctgcggacg gcccaactgc ccggtggaga
     2161 agcgacccta cccccccggg gagcacggtc gcgcccgacg gcgtgagacc gagtaccagg
     2221 tgcggctggc cgagaagcag aagctccgcg cgatctacgg gatcggggag cggcagttcc
     2281 ggaagtactt cgccaaggcc tcgcggcgcc acggcgtcac cggcgaggcc ctcttccaga
     2341 tgctggagac gcggctcgac agtctggtcc ttcgcctggg gctggctctg agccagcggc
     2401 aggcccgcca gctcgtctcg cacggccacg tgctggtcga cgggcgccgg gtctcgatcc
     2461 ccagcgcgca ggttcggccg ggccagacgg tcacggtcgc ggacaaggcc cgcaacttcg
     2521 tgtcggtccg cgaggcgctc gaggtggcgc ccgacgtccc cccctacctg agccgaaccg
     2581 aggccgcggg gggcaggctg gagcgcctgc ccgagcgcgg ggagatcccg ctccccgtcg
     2641 aggtggacga gcgtctcatc gtcgagtact acgcctgagc cctccggctc catgagcgag
     2701 ccgatcctcg aagcccggaa ggtggtgaag acctaccaca cgggggcggc cgacgtgcag
     2761 gcgctgcgcg gcctcgacct cacggtccac cccggcgaga tggtggcggt cgtgggcccc
     2821 tcgggctcgg gcaagaccac cctcctcaac tgcctctcgg gccttgacga gatcgacgag
     2881 ggcgaggtct ccatcgcggg tcaggagatc cactcgatgc ccgaccgggt gcgcaccagc
     2941 ttccgaggcc gcgagatggg cttcgtgttc caggccttca acctgatcca ggtgttcacg
     3001 gcggccgaga acgtcgagct gccgctgctg ctgaacggcg tgccgccaag ggaggcgcgc
     3061 gtgcaagcgg agaacgtgct cgatctcgtg ggcctcggcc atcggctggg ccaccgcccc
     3121 ccggagctct cgggagggga gcagcagcgg gtggcgatcg ctcgggccct ggcggccggc
     3181 ccctcgatcg tgtgggccga cgagcccacg gggaacctgg acagcgtcac ggccgagcag
     3241 gtgatgacgc tgctgcgtcg cctgaacgag gaaggcctca cggtgatcct cgtcacgcac
     3301 gacctggcga tcgcgcggac ggcgtcgagg atcgtctcga tgcacgacgg ccagatcgtc
     3361 ggcgacgagc ccctgccatg aggtggagac ggccgctgaa ggggcaggcg tgcaggaggc
     3421 gtccgccgtg gctgggtctc gccctcgtgg ccgtgctcct ggtcgcggct acgctggcgc
     3481 ttcccagcct cctgcccggc ctcctgccgg ggacccttcc gtgaacccca cgtacctccc
     3541 ggcgctggcg atcctcgccg gcctcctggg cctgctggcg atcgtcaccg cggcccgcca
     3601 cgcggtcccg cggcgtctcg cgctccgatc catcgcccgg cggccggggg agaccgtcct
     3661 cgtcatcctg ggatcgctcc tggggaccgc gatcatcacc gggtcgttca tcgtggggga
     3721 cacgctggac agctcgatcc gggccagcgc cgagacccag ctgggtccga tcgacgagac
     3781 ggtcacgggg ttcgggaccg agtccctgcc cctgctgcgc gaggcgctcg acgggctcga
     3841 ggacgagccg ggcgtcgacg gcgtgacctt cgggatccgg gccgccggca ccgccgccgt
     3901 ccgcctggga accgacgacg tcgccgtgca gccccgcctg ctcgtcctgg agatggacct
     3961 cgaggaggcc ggctcgttcg gcggcgaccc cgccgccacg ggcctcgacg gcgcggtgac
     4021 gcccgcccag ggcgaggccg tgatcaccga ggacctcgca gcgcacctcg acgtcgagcc
     4081 aggagacgag atcaccctct tcgcctacgg gagcgagcgc gagctgcgcg tcaccggcct
     4141 ggtccctcgc ctgggcgtcg ccgggtacac caccgagttc agcgccgaga gcttcaacgt
     4201 cttcgtggcg cccgggacgc tggaggagct ggtggcggcc gcgccgcccg acctcgaggc
     4261 ctcgcccccc acgtcgctcg cgttcgtctc caacgagggg ggcgtgttcg agggcgagga
     4321 gccgaccgat gaggtcgttc cgctgatccg ggagcggatc gagggcctgg agggcgtgga
     4381 cgtcgtggac agcaagcgcg acgtcgtgga cgcggccgac cggtcgggga gccagttctc
     4441 ggagctcttt ctctcgatcg gatccttcgc cgtcatcgcc gggatcctgc tgctcgtcaa
     4501 catcttcgtg atgctctccg aggagcggaa gggcgagctg ggcatgatgc gggcggtcgg
     4561 gatgcggcgg ggccagctcg tgcgctcctt ctacatcgag ggctcgttgt acgggctggt
     4621 ggcctcggcg atcggggcgg tggcggggat cgcggtgggg gcggcgatcg ttcgcgtggc
     4681 ggcggacatc ttcgcggggg ccggggagtt ctcgctggag ctcgtcctgt cggcgccgct
     4741 ggtgagcatc gtggggggct tcctgatcgg cctggcgatc tccctcctga ccgccctctt
     4801 caccagcgcg cgcatcagcc gcctgaacat catccgggcc atccgggatc tgcccgagcc
     4861 gcccgcccgg cggcaccgcc tgcgcggact cgtgctggga gcggtggccc tgatggtcgg
     4921 gggagggatg acggccttcg ccatcgtcga gggagaggcg gcctcggcct tcgccgggcc
     4981 gggcatcctc gccctgggcc tggcgggcct gctgtggcgt ctcgtcccgc gaaggccgct
     5041 gggctcggtc ctgggcctgg cggtcctggg ctgggggatc ttcgggccga cactggctcc
     5101 cgaggtcttc ggagaggccg acatccccgt gttcgtgatc cagggcgtgc tgctgacggc
     5161 ggcggccgtg gcggtgctcg ggttcaacca ggagtcggtt ggtcacgcgg tccgcgcggt
     5221 ggccgggggc gcccgcaacc tcgccgtgcg gctggggctc gcctacccgc tggccaagcg
     5281 cttccgcacg gtgatgaccc tctcgatgta cgcgctcgtc gtgttcacgc tcgtgttcat
     5341 ctcggtgctg tcgaacatct tcgggggcca ggtcgaggac ttcacccagc aggaggccgg
     5401 cggctacgac ctgctggtgc ggtcgtcggc ggcgaacccc ttgacggccg atgcggccgc
     5461 ggccgaggac ggcgtggagg ccgcgtcccc catccgctac gcggccttca gcgtcgagtt
     5521 cagcacggag ctccagcccg aggccgagcc gtgggcgctc tcgggggtcg aggagggctt
     5581 cctggcccag ggtcccccgg cgctggagga gtacgccccc gagtacggga gcgagggcga
     5641 gacctgggcg gctctgctgg ccgatccgtc gctgatcatc ctggacggat tcttccttca
     5701 gcagggaggg cccgaggctc cgcccgccga tctcggcgac gccgtcacga tccgcgatcc
     5761 cctgacgggc agggaggtcg agcgcacggt gatcggcgtg acgcggggcg gcttctcgtt
     5821 ctcgggcccg ttcgtgtctg aggagagcat cctggagctc gtcggcccgc gggccgtggc
     5881 cacccgcctc tacgtgacgc tcgagcccgg ccccgatccc gtggaggtgg ccacgcggct
     5941 ggaggaggag cacatcccga gcggggtgga ggccaggacc ttccgggcca tcgtctcgga
     6001 gtcccagtcg gtgaacctgc agttcatccg gctgatgcag ggctacctgg cgctcggcct
     6061 ggtcgtgggg atctcggggc tgggcgtgat catggtgcgc gcggtccggg aacggcggcg
     6121 cgagatcggg atcctccgca gcctcgggtt ccaatcaggg acggtggggc gctcgttcct
     6181 cctcgagtcg gccttcgtgg cgctggaggg cctggtggtg ggagccgccc tggccatcgt
     6241 gacctcgtac cagctcgtgg agaacgcgga gatcttcggg gatctggacg tcacgttcgt
     6301 gatcccgtgg ggtgagatcg cgatcgtgct ggcggcgacg ctggtggcct cgctgctggc
     6361 gacggggtgg ccggcgcgcc aggccagccg gatccgtcca gcggtggccc tccgcgtcgc
     6421 ggattgaact tgtctcggga cggcctctcg gggtacgggt agagcagtgt ccgcggaacg
     6481 gaaggaggga tcgtccatgc gacggatgct cgtcgccgcc atcgtggcgg cgttcgtggt
     6541 cggcgcggcc gggatcgccc aggccgatca cacgcacgtc aggatcctgc cgaccggcga
     6601 ctgcgtcgtc ctggccgccg acgggaacga gaagtacatc gtgctcccgg acgagaaccc
     6661 gcaggtcgac gccgagctgc ccgagaatcg gaagcatccc ctgcacgtcc tcgtgcacct
     6721 cggtcagccc ggctcggacg gctccatcgc cgtgatgggc tctgcccagg acccctgcag
     6781 cgaggccggt ggccagaccg gcagctacgt gaacccgtag cggcggcttc ccttcagcga
     6841 gggggcccgg cgagacgtgc cgggtcccct cgttcgtggt aggaatccgc agatgcgcgt
     6901 ctgcctgatg gtcgagggcc aggagggcgt gggctgggat gactggctgg cgctggccag
     6961 ggcctgcgag gaccatgggc tcgaggggct cttccgctcc gatcactacc tctccgtgga
     7021 ggacgacgag acgcggggat cgctcgacgc gtggacgacc ctggcagcgc tggccgctga
     7081 gaccgaacgg atccggctcg gcacgctcgt ctcccctgtg accttccgcc atccgtcgct
     7141 gctggcgaag gcggccgtga ccgccgacca cgtgtcgggc gggagggtgg agctggggat
     7201 cggggccggg tggcacctgc gggagcacga ggcgtacggg ttccccttcc cggagccccc
     7261 cgagcggatg gacctgctgg ccgagcagat cgagatcgtg caccgccagt ggagcgaaga
     7321 gatggtctcg ttccagggga agcactaccg gctcgaggcc gtcaaggcgt tgccgaagcc
     7381 ggtgccggac ccgcacccgc cgctgatcat gggaggccac gcgaagccgc gctcggtcga
     7441 gctctccgcc cggtgggccg acgagtacaa cgtcttcttc tcgacgcccg aggagtgccg
     7501 ggcgaagcgg gcgcgcgtcg aggaggc
//
LOCUS       DASUTZ010000229         5011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1374560, whole genome shotgun sequence.
ACCESSION   DASUTZ010000229 DASUTZ010000000
VERSION     DASUTZ010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374560"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1317
                     /locus_tag="VF097_09920"
     CDS             <1..1317
                     /locus_tag="VF097_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX6263150.1"
                     /translation="NHTSDEHPWFRAARDPDSPYRDHYVWAETPPDDAESGVVFPGVQ
                     DSTWTLDERSGEHYFHRFYEFEPDLNVANPDVRAEFRRIMGYWIQQGVAGFRVDALPF
                     LIEPAGVERPKPHPRFEYLKELREFLQWRRGDAVLLGEANVERDRVDDYFTTGGMHMI
                     FNFPLNQLVWLALAKGEAGPIVRGLEHTAGIPATDQWATFLRNHDEVDLGRLSEEDRQ
                     LVFRVFGPEPSMQLYDRGIRRRLAPMLGGDRRRIEMAMSLLFSLPGTPVILYGDEIGM
                     GDLLSLDEREAVRTPMQWNDEENAGFSTADPDRMARPVISEGDYAYERVNVAAQMRDA
                     GSLLSWMKRLIHTRREAPEVGLGDWQLLETGNEHVIGLRAQWEGREVAVYHNLDGEPQ
                     KARMGLEPTELSHPLELFGDRDYEEGWAPFELAGYGYRWARIRGVA"
     gene            complement(1333..3345)
                     /locus_tag="VF097_09925"
     CDS             complement(1333..3345)
                     /locus_tag="VF097_09925"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263151.1"
                     /translation="MRHALVALTALSLLLVPQRGGAVPGDLHLSSISDEGVKARWSVL
                     PSLSADGTRVAFQSSSRLHPADGDDVVDVYVRDLTTGRTILASTSATGEKANGRTSLP
                     AISADGNRVAFASGATNLHPSGSRGLMVKDLLTGEVFLVSAHGEYPVLSADGTRVAFQ
                     TTGTGLDPRDDDEASDVYVKDLATGELFLASTSSDGVKANGDFFHGSGGASLSADGRL
                     VGFASGATNLHPGDEDEVIDVYVKDLASGLISLISTSADGTKADDDSFSPSLSADGDV
                     VAFRSYSSRLVPGFSGGFGQIFVKDLTTDEIVPASLAADGTRADRGAADPSLSADGKR
                     VAFSSTSANLHPGDGDVVSDIFVKDLATSALELASTSNSGIKSNGISYQVSLSGEAAA
                     VAFRSDATNLDPRDGDSTPDVFVKELGGAAASPLVADLSIRQTDAPDPVPAGENVTYE
                     IRVENAGPSAATGVTLVDELPEGIEFRSATASQGSGCIRSDDLVRCDLGALQPGASAA
                     ATVIVAPQGLGYLTNTVTVHGNEPDPRTFDNRSSLNTDVDRAADVALSMTDAPDPASV
                     RSSITYSITVQNLGPTWASLELVDALPDGSRLESVDAGGSRCETSRQEVRCWIFGIPD
                     GGSTTVRVTVSARKPATLTNTATAFPLDFDADASNNTTKESTVVTR"
     gene            3465..4028
                     /locus_tag="VF097_09930"
     CDS             3465..4028
                     /locus_tag="VF097_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016433793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX6263152.1"
                     /translation="MNALSDESLLAGLAAGDPEGSTAFVRRFQGRVYGLALTIVGDPR
                     VAEDVAQEAFVRAWRHAELYEPSRGRVVAWLLRITRNVALDALRVRRATPVDPEALLG
                     LAAAREGDPEARAVVSLELHGLRSDLLSLPDEQRRAVLLAAFYGRTAREIGEIEGIPL
                     GTAKTRIRSGMRRLREALEVRDGRERM"
     gene            4009..4698
                     /locus_tag="VF097_09935"
     CDS             4009..4698
                     /locus_tag="VF097_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263153.1"
                     /translation="MGENGCELTHGIAAELALGVALGEERDRALRHVEECASCRRGLV
                     ELGELVDELMLLAPEREPPVGFESRVVSSATQDRRPPRWRRFVASVAAAAIAAAATGT
                     AAHVLTRDERTVAAQFRTALTRAGGQYFGVEFLHEEGGGRAGHVFVYRGDVSWAFVVP
                     SGELPGSFRVEMTTRDGRTLEAGGIDPAGEGGGAGLVLPVDLEVIESIRLIPVGGGDA
                     LEAHMPEPPEA"
     gene            4721..5002
                     /locus_tag="VF097_09940"
     CDS             4721..5002
                     /locus_tag="VF097_09940"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX6263154.1"
                     /translation="MAALTAEQIAEDLRELPGWRHEGGEIFKWFRFDGFPEAIAFLQR
                     IVEPAERMNHHPDIENHYDRVRIGLHTWSEEAVTEKDLALAREIERLAG"
BASE COUNT          765 a         1638 c         1852 g          756 t
ORIGIN      
        1 aaccacacat ccgacgagca cccctggttc cgggccgcgc gggacccgga ctcgccctac
       61 agggaccact acgtctgggc ggagactccc ccggacgacg ccgagagcgg cgtggtgttc
      121 ccgggcgtcc aggactcgac gtggaccctc gacgagcgat cgggcgagca ctacttccac
      181 cgcttctacg aattcgaacc ggacctcaac gtcgccaacc ccgacgtccg ggccgagttc
      241 cgcaggatca tggggtactg gatccagcag ggcgtcgcgg ggttccgggt agatgcgctc
      301 ccgttcctga tcgagcccgc gggggtggag cgcccgaagc cgcatccccg tttcgagtac
      361 ctgaaggagc ttcgagagtt cctgcagtgg cgaagggggg acgcggtgct gctcggggag
      421 gcgaacgtcg agcgggaccg ggtggacgac tacttcacca cgggcggcat gcacatgatc
      481 ttcaacttcc ccctcaacca gctcgtctgg ctggcgctgg cgaaagggga ggcgggcccc
      541 atcgtccgcg ggctcgagca tacggccggg atcccggcca ccgatcagtg ggcgaccttc
      601 ctccggaacc acgacgaggt cgacctgggg cggctctccg aggaggatcg acagctggtc
      661 ttccgggtgt tcggccccga gccgtcgatg cagctctacg accgcggcat caggcggcgg
      721 ctggccccga tgttgggggg cgaccgacgc cggatcgaga tggcgatgag cctcctgttc
      781 agcctccccg ggacgccggt catcctgtac ggggacgaga tcggcatggg cgacctgctc
      841 tcgctcgacg agcgggaggc ggtgcggacg ccgatgcagt ggaacgacga ggagaacgcc
      901 ggcttctcca ccgcggaccc ggatcgcatg gcccgccccg tgatctccga gggcgactac
      961 gcgtacgagc gagtcaatgt cgccgcccag atgcgggacg ccgggtccct cctgagctgg
     1021 atgaagcgct tgatccacac gcggagggag gcgccggagg tgggcctcgg cgattggcag
     1081 ctcctcgaga ccgggaacga gcatgtcatc ggcctccgag cgcagtggga ggggcgcgag
     1141 gtggccgtct accacaacct cgacggcgag ccgcagaagg ctcggatggg cctcgagccc
     1201 acggagctct cccacccgct cgagctcttc ggcgaccgcg attacgaaga gggatgggcc
     1261 cccttcgagc tggccgggta cgggtaccgc tgggcgcgga tccgcggcgt ggcctgaccc
     1321 gctcgggggg cgtcatcgcg tgaccacggt cgactccttt gtcgtgttgt tcgaggcatc
     1381 cgcatcgaag tccagaggga acgccgtcgc cgtgttggtc aaggtggcgg gctttcgagc
     1441 ggataccgtg acgcggacgg tcgtcgatcc gccgtcgggg atcccgaaga tccaacatcg
     1501 cacctcctgt cgcgacgtct cacagcgaga cccccccgcg tccacggatt ccaggcgcga
     1561 cccatcgggg agtgcatcca cgagctcgag ggacgcccac gtcggtccca ggttctggac
     1621 cgtgatcgag tacgtgatgg agctccgcac gctggccgga tcgggggcgt cggtcatgga
     1681 gagggccaca tccgccgccc gatccacgtc ggtgttgagg gaggaccgat tgtcgaacgt
     1741 ccgagggtcc ggctcgttcc catgcaccgt taccgtgttg gtcagatagc cgaggccctg
     1801 gggcgcgacg atcaccgtcg ccgcggcgga cgctccgggc tggagcgccc ccagatcgca
     1861 tcggaccagg tcgtcggaac ggatgcagcc cgagccctgg ctggctgttg ccgaccggaa
     1921 ctcgatgccc tcgggcagct cgtcgaccag cgtgacgccg gtggccgccg agggtcccgc
     1981 gttctcgacc cggatctcgt aggtgacgtt ctcgcccgcg gggacggggt ccggagcgtc
     2041 ggtctgccgg atggagaggt cggcgaccag cggggaagcc gctgcaccgc ccagctcctt
     2101 cacgaagacg tccggcgtcg agtcgccgtc ccgcggatcg aggttcgtcg catcggatcg
     2161 gaacgccacc gcggcggcct cgccgctgag cgagacctgg tagctgatcc cgttgctctt
     2221 gatccccgag ttcgaggtcg aggcgagctc cagcgcgctc gtcgccaggt ccttcacgaa
     2281 gatgtccgag acgacgtcgc catcgcccgg atgaaggttg gctgacgtcg agctgaaggc
     2341 aacgcgcttt ccgtccgcgg acagggacgg atcggcggcc ccgcggtcgg cgcgcgttcc
     2401 gtccgccgcc agggaggcgg gcacgatctc gtcggtcgtg aggtccttca cgaagatctg
     2461 accgaaccct ccggagaacc cggggacgag tctggacgag taggagcgga acgcgaccac
     2521 gtctccgtcg gccgagagcg aggggctgaa gctgtcgtcg tcggccttcg tgccgtccgc
     2581 ggaggtggag atgagactaa tcagcccact ggccaagtcc ttgacgtaga cgtcgatgac
     2641 ctcgtcctcg tcccccggat gcaggttggt ggcgcccgag gcgaacccga ccagcctccc
     2701 gtccgcggag agcgacgccc cgccggagcc gtggaagaag tcgccgttgg ccttgacgcc
     2761 gtccgaggag gtcgaggcca ggaacagctc gccggtggcc aggtccttca cgtacacgtc
     2821 cgacgcctcg tcgtcatctc gggggtccag gccggttcct gtcgtctgga acgccactcg
     2881 ggtgccgtcg gcggagagca ccgggtactc tccgtgagca gatacgagga agacctcgcc
     2941 ggtgagaagg tccttgacca tgagtcctct cgatccggac gggtgcaggt tggtcgcgcc
     3001 ggaagcgaag gccacacgat tgccgtccgc ggagatcgcg gggagcgagg tgcgcccgtt
     3061 cgccttctcg ccggtcgccg acgtcgaggc gaggatcgtc cgcccggtgg tgaggtctcg
     3121 cacgtagacg tccacgacgt cgtcgccgtc tgcggggtgg agccgagacg aggactggaa
     3181 cgcgacacgc gttccgtccg ccgacagcga cgggagcacg ctccatcgag ccttcacccc
     3241 ctcgtcggag atagaggaga gatggaggtc tccgggaacg gcgcctcccc gctgaggaac
     3301 gagcagcagg ctcagggccg tgagggcgac gagcgcgtga cgcatggatc ctccttcggt
     3361 cgggacgtcg gaggtactac tcgctgacgt tcgcgggggg attgcctccc atcgacattc
     3421 gctcgacagc aatccatcga agggtccgcc ccgtatatct tgcggtgaac gcgctctccg
     3481 acgagtccct cctggcgggt ctcgcggcag gcgatccaga ggggtcgacg gcgttcgtgc
     3541 ggcggttcca gggtcgcgtc tacgggctcg ccctgacgat cgtgggcgac ccccgggtgg
     3601 cggaggacgt cgcccaggag gcgttcgtgc gtgcgtggag gcacgccgag ctctacgagc
     3661 cgtctcgggg ccgggtggtg gcgtggctgc tgcggatcac gcggaacgtc gcgctcgacg
     3721 cgctccgcgt acgccgggcc acgccggtgg accccgaggc gctgctgggg ctggcggccg
     3781 cgagagaggg ggatccggag gcccgcgcgg tcgtgagcct cgagctccac ggcttgcgat
     3841 cggatctgct ctcgctgccg gacgagcagc gccgggcggt cctcctggcg gccttctacg
     3901 ggcgcacggc gcgagagatc ggggagatcg aggggatccc gctgggcacc gcgaagacgc
     3961 ggatccggtc ggggatgagg aggctgaggg aagcgctcga ggtgcgggat gggcgagaac
     4021 ggatgtgagc tgacgcacgg gatcgcggct gagctggcgc tcggcgtggc cctcggcgag
     4081 gagcgggatc gggcgctccg acacgtggag gagtgtgcct cgtgccgacg gggcctggtc
     4141 gagctcggtg agctcgtgga tgagctgatg ctgctcgctc cggagcgaga gcccccggtg
     4201 gggttcgagt cccgcgtggt gtcgtcggcg acccaggatc gtcgacctcc ccgctggcga
     4261 cggttcgtcg cgtccgtcgc ggcggccgcg atcgcggccg ccgcgacggg gacggcggcc
     4321 cacgtcctga ccagggacga gaggaccgtc gccgcccagt tccggaccgc gctgacccgg
     4381 gcaggcggcc agtacttcgg cgtcgagttc ctccacgagg agggaggagg gagggcgggc
     4441 cacgtcttcg tgtaccgagg ggacgtctcc tgggcgttcg tcgtcccgtc gggggagctc
     4501 cccgggagct ttcgggtcga gatgaccacc cgcgacgggc gcacgctcga ggcgggaggc
     4561 atcgatcccg cgggggaggg cggtggggcc ggcctcgtcc tgccggtcga cctggaggtg
     4621 atcgagtcga tccgtctgat ccccgttgga gggggggacg ccctcgaggc gcacatgccg
     4681 gaaccccccg aggcctgatc cgagcggtac gatccggccc atggctgcgc tgactgccga
     4741 gcagatcgcc gaggacctcc gcgagctccc gggctggagg cacgagggcg gggagatctt
     4801 caagtggttc cgcttcgacg gattccccga ggcgatcgcc ttcctccagc ggatcgtgga
     4861 gcccgccgag cgcatgaacc accatccgga catcgagaac cactacgacc gggtgcggat
     4921 cgggctgcac acgtggagcg aggaagccgt caccgagaag gacctcgcgc tggcccgaga
     4981 gatcgagcgg ctggccgggt aggctcgccg a
//
LOCUS       DASUTZ010000230         3795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_81335, whole genome shotgun sequence.
ACCESSION   DASUTZ010000230 DASUTZ010000000
VERSION     DASUTZ010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81335"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /locus_tag="VF097_09945"
     CDS             <1..1158
                     /locus_tag="VF097_09945"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6263155.1"
                     /translation="AEGFAGLALWSYFLAVMGDATYRFDATSGQLGILLASFSITFIP
                     STPPFGALADRWSPQRMMVVAIAAFAGSLVVASLAPSLPWLYLSMAWVGLAEGVLWPA
                     RGALVPRLVERDRLVRANGMISLVRELPMALGPLMGAVAVGLWGRQAPYWVGLVALAF
                     ALLFYALVPDRRLDGRAHDSFLRDLGAGLRAGMRVPVLRLLFLTGFAAMLVLGLMQTL
                     EPLFVKDVLDLGQRSLGFLWSMQGVGALLASILLIGLRRATGAEMVVIACGVLIAGAG
                     LLVYAATGVFGVAIPGAILFGFGFPLFYSTGQALIQRISSAPGKVSAVFVVISEVGPL
                     VAALGIGVLGRVDVQVWLVGAAGGLLVFGGMGLWASRRPALLVEEAPASAS"
     gene            1155..2336
                     /locus_tag="VF097_09950"
     CDS             1155..2336
                     /locus_tag="VF097_09950"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018500069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX6263156.1"
                     /translation="MIPPPEPILHVDMDAFYASVEVLKDPTLKGKPVVVGGSGSRGVV
                     MSASYESRRFGIRSAMPAARARRLCPEAVFVRPDFSSYRAYATRLREIFLSFTPLVEP
                     LSLDEAFLDVGGASRLFGEPVEIAEKVRARVLEETELVCSVGVAPNKFLAKMASTHAK
                     PDGLLVVPAGGVEAFLHPLPVDALWGVGEKTDELLRRLGVRTIGELAVTSPRVLERVL
                     GEQQGRHLAALARGEDDRSVIPYEAPKQVSHEETFDRDLDAEDDIRRELLRLSFRVAA
                     RLRKEGFRARTVTLKARLASFTTLTRSRTLPDPTDVGNDLYRVVAELFGALPSRGRLR
                     IRLLGVGAAGLRPSGVDQLALVRAGRWEDAERALDRIERRFGEGAAFPASLLDRGRAR
                     G"
     gene            2395..2772
                     /locus_tag="VF097_09955"
     CDS             2395..2772
                     /locus_tag="VF097_09955"
                     /inference="COORDINATES: protein motif:HMM:NF022684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HEX6263157.1"
                     /translation="MPLSDHEQRILEEIEKRLREEDPRLAETVAKATVHAHIVRRVRL
                     GIVAFVAGFVMLMLFMVSIWVALAGFGVMLASALFVYNTIRRMAHDQMEAARREGGPS
                     VIGSLARLVDRFRGRSEPPADQN"
     gene            2888..3376
                     /locus_tag="VF097_09960"
     CDS             2888..3376
                     /locus_tag="VF097_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX6263158.1"
                     /translation="MEGFTSHQASRLTGCTRRQLRYWDEIGLVKPSVQSTGGRPGVPR
                     LYSFRDLIALKVVKSLLDGGMSLQRVRRAYGFLRERAGMDVHLSEVKLITDGKSIFKV
                     ARREGEILDALRNGQMAFFVALDSIVRSIDAEVEEFRQDRRQFERALREATRQVQVEA
                     AT"
     gene            complement(3373..>3795)
                     /locus_tag="VF097_09965"
     CDS             complement(3373..>3795)
                     /locus_tag="VF097_09965"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEX6263159.1"
                     /translation="ASIWPRIALGVLLVLLAIGGVPTWKWISRRRRLRRASSPREAVL
                     AAFDVLEERAADVGLERRLEETLLEYEARLRGSVRLRDGDLERMTGLVTRAAYGSDEV
                     DAAEARLAADALKILTADLRRHAGRGRALVGAIRPVRD"
BASE COUNT          435 a         1305 c         1428 g          627 t
ORIGIN      
        1 gccgagggct tcgccggcct cgccctctgg agctacttcc tggccgtgat gggcgacgcc
       61 acctaccgct tcgacgccac ctcgggccag ctcgggatcc tgctcgcctc gttctcgatc
      121 acgttcatcc cctccacgcc cccgttcggg gcgctggccg accggtggag ccctcagcgg
      181 atgatggtcg tggcgatcgc ggcgttcgcc gggtcgctcg tggtcgcctc gctcgcgccg
      241 tcgctgccct ggctctacct gtcgatggcg tgggtggggc tggccgaggg cgtgctgtgg
      301 ccggcccggg gcgccctcgt cccccgcctg gtcgagcgcg accgcctggt gcgggccaac
      361 gggatgatca gcctcgtccg cgagctgccg atggccctcg gtccgctgat gggggcggtc
      421 gcggtggggc tgtggggacg ccaggctccc tattgggtgg ggctcgtggc gctcgctttc
      481 gccctgctct tctacgcgct cgtcccggac cggcgcctcg acggacgggc gcacgactcg
      541 ttcctccggg acctgggggc ggggctccgg gccgggatgc gcgtccccgt cctgcgcctg
      601 ctgttcctca cggggttcgc cgcgatgctc gtcctggggc tcatgcagac gctcgagccg
      661 ctgttcgtga aggacgtgct cgatctcggg cagcggtccc tcggcttcct gtggtcgatg
      721 cagggcgtcg gcgcgctgct cgcctcgatc ctgctgatcg ggctccggcg ggcgaccggc
      781 gcggagatgg tggtgatcgc gtgcggcgtc ctgatcgcgg gcgcgggact cctcgtgtac
      841 gcggcgaccg gtgtgttcgg ggtggcgatc ccgggcgcga tcctgttcgg gttcgggttc
      901 ccgctcttct actcgacggg tcaggccctg atccagcgga tctcgagcgc gccggggaag
      961 gtcagcgccg tcttcgtcgt gatcagcgag gtgggaccgc tcgtggcggc gctcggcatc
     1021 ggcgtcctgg ggcgggtgga cgtgcaggta tggctggtcg gcgccgcggg gggcctgctg
     1081 gtgttcggcg ggatgggtct gtgggcctcg cgccgtcccg ccctcctggt ggaggaggcc
     1141 cccgcgagcg cgtcgtgatc cctcctcccg agcccatcct gcacgtggac atggacgcgt
     1201 tctacgcgtc ggtcgaggtc ctgaaggacc ccacgctgaa ggggaagccg gtcgtcgtgg
     1261 gaggctcggg gtcgcgcggc gtcgtgatgt cggcctccta cgagtcccgg cggttcggga
     1321 tccggtcggc gatgccggcc gcccgcgccc ggcgcctctg tcccgaggcg gtcttcgtcc
     1381 gtccggactt ctcctcctac cgggcgtacg cgacgcgcct ccgcgagatc ttcctttcgt
     1441 tcacgccgct cgtggagccc ctctccctgg acgaggcgtt cctcgacgta gggggcgcct
     1501 cgcggctgtt cggggagccg gtcgagatcg ccgagaaggt ccgggcccgc gtcctcgagg
     1561 agaccgagct cgtctgctcc gtgggcgtgg cgccgaacaa gttcctggcg aagatggcct
     1621 ccacccacgc caagcccgac ggtctgctcg tcgtgcccgc gggcggcgtc gaggcgttcc
     1681 tccacccgct ccccgtggac gcgctgtggg gcgtggggga gaagaccgac gagctgctcc
     1741 ggcggctggg ggtgcggacg atcggagagc tggccgtgac ctcgccgcgc gtcctggagc
     1801 gggtgctggg ggagcagcag ggccggcacc tcgccgcgct ggcccgcggc gaggacgacc
     1861 gttcggtgat cccgtacgag gctccgaagc aggtcagcca cgaggagacc ttcgaccggg
     1921 acctcgacgc cgaggacgac atccgtcggg agttgctgcg gctctcgttc cgggtggcgg
     1981 cgcgactgcg gaaggagggg ttccgtgcga ggaccgtgac cctcaaggcc cggcttgcca
     2041 gcttcacgac cctcactcgg tcgaggacgc tgcccgaccc gaccgacgtg ggcaacgatc
     2101 tctaccgggt cgtggcggag ctcttcgggg cgcttccctc acgcgggcgc ctgcggatcc
     2161 gcctgctggg ggtgggggcg gcgggcctgc gcccatcggg cgtggaccag ctcgcgctgg
     2221 tacgggccgg ccgctgggag gacgccgagc gggcgctcga tcggatcgag cggcggttcg
     2281 gcgagggcgc cgccttcccg gcctcgctcc tcgatcgggg ccgggcccgt ggttgaggga
     2341 acccccaagg tagggtactc cctataattg ggcgcgagga aaggagcggc cggcatgccc
     2401 ctgtcggatc acgagcagcg catcctcgag gagatcgaga agcgtctccg ggaggaggac
     2461 ccacgtctgg ccgagaccgt ggccaaggcc accgttcacg cccacatcgt gcgtcgggtc
     2521 cgcctgggga tcgtcgcttt cgtggcgggc ttcgtgatgc tgatgctgtt catggtgtcg
     2581 atctgggtgg cgctcgcggg gttcggggtg atgctggcgt cggcgctctt cgtctacaac
     2641 acgatccgac ggatggccca cgatcagatg gaggcggccc gtcgcgaggg cgggccgtcg
     2701 gtgatcgggt ccctggcccg tctggtcgac cggttccgcg gccgctcgga gcctccggcc
     2761 gaccagaact gatcgccctc cttgcttgcg catggggtcc ccgttgccgt acaactacgg
     2821 ctaccacttc ccccgggggc cacgcggtcg gaaagctccc gcccgggcga gttcgatcgg
     2881 aggttcgatg gagggcttca cgtcgcatca ggcatcgcgc ctgaccggct gcacccgcag
     2941 acagcttcgc tattgggacg agatcgggct cgtgaagccc tcggtgcaga gcaccggcgg
     3001 tcggcccggc gtcccgcgtc tctactcgtt ccgcgacctc atcgcgctga aggtcgtgaa
     3061 gtcgctcctc gacgggggga tgtcgctgca gcgcgtccgt cgcgcctacg ggttcctgcg
     3121 cgagcgcgcg gggatggacg tccacctgtc cgaggtcaag ctcatcaccg acgggaagag
     3181 catcttcaag gtggcccgcc gcgagggtga gatcctcgac gctctccgaa acgggcagat
     3241 ggccttcttc gtggcgctgg actcgatcgt gcggtcgatc gacgcagagg tcgaggagtt
     3301 ccggcaggac cggcgccagt tcgagcgggc gctccgggag gccacgaggc aggtccaggt
     3361 cgaggcggcg acctagtcgc ggacggggcg gatcgccccc acgagcgcgc ggccccgccc
     3421 ggcgtgccgc cgcaggtcgg cggtgaggat cttgagggcg tcggcggcga ggcgcgcctc
     3481 cgcggcgtcc acctcgtcgc tcccgtaggc cgcgcgggtc acgagccccg tcatccgctc
     3541 caggtcgccg tcgcgcagcc tgaccgagcc gcggaggcgc gcctcgtact ccaggagggt
     3601 ctcctcgagg cgccgctcga ggcccacgtc cgcggcgcgc tcctcgagga cgtcgaaggc
     3661 cgccaggacg gcctccctcg gggacgaggc gcgccgcagg cggcgccggc gtgagatcca
     3721 cttccaggtc ggcacgccgc cgatggccag gagcacgagc aggacgccca gggcgatccg
     3781 gggccagatc gatgc
//
LOCUS       DASUTZ010000231         4845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_81606, whole genome shotgun sequence.
ACCESSION   DASUTZ010000231 DASUTZ010000000
VERSION     DASUTZ010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81606"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VF097_09970"
     CDS             complement(<1..224)
                     /locus_tag="VF097_09970"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX6263160.1"
                     /translation="MRYRPTVAVIDLEAVRHNVRALSREGSAYMAVVKANGYGHGAVP
                     IARAALEAGAEWLGVALVEEGVALREAGITA"
     gene            complement(241..1770)
                     /locus_tag="VF097_09975"
     CDS             complement(241..1770)
                     /locus_tag="VF097_09975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX6263161.1"
                     /translation="MRPVLTAAQMRAADRAAAERGVPVEALMERAGHAVARAVAALLG
                     GTYGRRIVALCGKGNNAGDAFVAVRLLAAWGAAPAVVSVAGLDELRAAAAANAERLRD
                     SGVPTIAWGTREADRAIARADVVVDGLFGSRFRGPVEGSWREAIEAVNASPAPVVAVD
                     IPSGVEGDTGAVRGPAVRAEVTVTLAAPKVGVVLYPGAEFAGRVEVADIGIPADLLES
                     ELALVEPGDVAAILPRRAPEDHKHRSGDVLVVAGSRRMTGAPRLVAEGAARAGAGLVH
                     VAVPEGILPMVQAGLTEAVFLPLPEGPDGSVAASAWGELKKRMDRFDAVAIGPGLTTE
                     GEAPDLVRALVRSSPVPIVADADALNAFAGRAQDLRERAADLVLTPHAGEFARLLGAD
                     PEEDRVGMAAAAAADTGAVMVLKGARTLVAPPGGEVRVNPTGSAALATGGTGDVLAGA
                     IAAYLARGLPPADAATAAVYVHGLAGQVVGRRMGEGGTAGDVAIAIPAAVEALLGSVA
                     S"
     gene            complement(1770..2147)
                     /locus_tag="VF097_09980"
     CDS             complement(1770..2147)
                     /locus_tag="VF097_09980"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:NF000832.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX6263162.1"
                     /translation="MRIVGVGVDIVDVTRVERMLERYPRFPERVFTPGEVAYCESKGN
                     RATSYAGRWAAREATRKALGGIRRMRWHDVRVERAPSGAPRIVLEGTAKERADALGVS
                     EVLISFTHERTMATAFAIAVGEG"
     gene            complement(2181..4004)
                     /gene="glmS"
                     /locus_tag="VF097_09985"
     CDS             complement(2181..4004)
                     /gene="glmS"
                     /locus_tag="VF097_09985"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX6263163.1"
                     /translation="MCGIVGYVGPDEALPILMDGLHRLEYRGYDSAGVAVLGDDLRVV
                     KRAGKLDELEKALAEEGQPPGRIGMGHTRWATHGPPTDQNAHPQTDCSGAIAVIHNGI
                     IENFFEHRERLEAEGHEFRSETDTEVVAHLLEGRYEGDLPAAVRSTVKELEGAYALVV
                     MAADQPDLVVGVRVSSPMIVGVGEGENLLASGAPAFLARTRFQVPLEDGEMVELRAEG
                     YRITDLEGNERSTEPEEIDWDIEQAEKGGFEDFMLKEIYEQPQAILDTARGRIREGKL
                     LLDEVRMSEEDTRAVDKVFVVACGTAFHAGLVAKYAIEHWTRLPVEIEIASEFRYRDP
                     VLDRNTLTLAISQSGETIDTLEAVRHAKHQRSWVTAITNTVGSSVAREADAVVYTRAG
                     PEIGVAATKTFTTQMVALHLVALYLAQVRGTMYPEEIQQVLDEMEGLPALVERTLELN
                     PGVEELARRYADARDVLFVGRHTGYPVALEGALKLKEISYIHAEGYPAGELKHGPIAL
                     LEPGVPVVAVATACHVYPKVLSNIQEIKARGAEVIALATEGDEEIGKLADHVLYVPRT
                     AELLSPVVVSVPLQLLAYHIAKVRGCNVDQPRNLAKSVTVE"
     gene            complement(4072..>4845)
                     /locus_tag="VF097_09990"
     CDS             complement(4072..>4845)
                     /locus_tag="VF097_09990"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX6263164.1"
                     /translation="PSLLRRLGADVIAIGDEPDGRNINVGCGATHPETVAEAVRRFKA
                     DAGVAHDGDADRAILCDAEGAIVDGDHMLGACALAMREEGTLARNTVVTTVMANLGFR
                     RAMEEAGISVVETQVGDRYVLEEMRRTGATLGGEQSGHVIFLDAATTGDGLLTAARFL
                     ALARSKGVSVAELASVVRKLPQVLEAVEVADRGGLEGSEEVRDAIRRAEEALGGRGRV
                     LVRASGTEPVVRVMVEAEEEEEARRHAEAIAAAVREALG"
BASE COUNT          641 a         1917 c         1627 g          660 t
ORIGIN      
        1 gcggtgatgc cggcctcccg gagcgcgacc ccctcctcga ccagggcgac gccgagccac
       61 tcggcccccg cctcgagggc cgcccgggcg atcgggaccg cgccgtgccc atagccgttc
      121 gccttcacca ccgccatgta cgccgagccc tccctggaca gggcgcggac gttgtgcctg
      181 acggcctcca ggtcgatcac cgcgaccgtg gggcggtacc tcacgcgctc accccccggg
      241 tcatgatgcc accgatccga ggagggcctc gacagccgcg gggatcgcga tggcgacgtc
      301 cccggccgtg cccccctcgc ccatccgccg gcccaccacc tggccggcga gcccgtgaac
      361 gtagaccgcg gcggtcgcgg catcggcggg ggggaggccc cgggccagat acgcggcgat
      421 cgcgccggcc agcacgtcgc cggtcccgcc ggtggccagg gccgccgacc cggtcgggtt
      481 gacccggacc tcccccccgg ggggagccac gagcgtccgg gcgcccttca ggaccatcac
      541 ggcgcccgtg tcggcggcgg cggcggcggc catccccacc cggtcctcct cggggtccgc
      601 ccccaggagc cgcgcgaact cccccgcgtg gggcgtgagg acgagatccg ccgcgcgctc
      661 ccggaggtct tgggcccgcc ccgcgaacgc gttgagggca tcggcgtcgg ccacgatcgg
      721 gacgggggac gagcgcacga gcgcgcggac gaggtccgga gcctctccct cggtggtcag
      781 ccccggcccg atcgccaccg cgtcgaatcg gtccatccgc ttcttcagct ccccccaggc
      841 ggaggcggcc accgagccat ccggtccctc gggcagaggc aggaagaccg cctccgtgag
      901 ccctgcctgc accatcggga ggatgccctc gggcaccgcg acgtggacga gaccggcgcc
      961 ggctcgcgcc gcaccctcgg ccacgaggcg gggggccccc gtcatgcggc gcgagccggc
     1021 gaccacaagg acgtcccccg accggtgctt gtggtcttcg ggggcccggc ggggaaggat
     1081 cgcggccacg tcgcccggct ccacgagggc cagttcgctc tccagcaggt cggccgggat
     1141 cccgatgtcg gccacctcga cccgtccggc gaactccgcc cccgggtaga ggactacccc
     1201 caccttcggg gcggcgaggg tcaccgtaac ctcggcccgc acggcgggcc cccgcaccgc
     1261 gccggtgtcc ccctcgacgc ccgacgggat gtccacggcc acgacgggcg caggcgaggc
     1321 gttgaccgcc tcgatggcct cccgccagga accctcgacg gggccacgga accgcgaccc
     1381 gaacagcccg tccaccacca cgtcggcccg cgcgatcgcc cgatcggcct cccgagtccc
     1441 ccacgcgatc gtggggacgc cggaatccct cagccgctcc gcgttcgcgg cggccgccgc
     1501 ccggagctcg tcgaggccgg ccaccgacac gacggccgga gccgcgcccc acgccgccaa
     1561 gagccggacg gccacgaagg cgtcgccggc gttgttgccc ttcccgcaga gggcgacgat
     1621 gcggcgcccg taggtgccgc cgaggagcgc ggcgacggcc cgggccaccg cgtgcccggc
     1681 tcgctccatc agggcctcca ccgggacgcc gcgctccgcg gcggcccgat ctgcggctct
     1741 catctgcgcg gccgtcagga ccgggcgcat cagccctctc ccaccgcgat cgcgaacgcc
     1801 gtggccatgg tccgctcgtg cgtgaacgag atcaggacct cggagacccc gagggcgtcg
     1861 gcccgctcct tggccgtccc ctcgagcacg atccggggcg ccccgctcgg cgcgcgctcc
     1921 acccggacgt cgtgccagcg catccgccgg atcccgccca gggccttgcg cgtcgcctcg
     1981 cgggccgccc atcggcctgc gtaggacgtc gcccggttgc ccttcgactc gcagtaagcg
     2041 acctcgccgg gcgtgaagac gcgctcgggg aaccgcgggt accgctcgag catccgttcc
     2101 acgcgggtga cgtccacgat gtcgacgccc acccccacga tccgcatcac gcgctcaggc
     2161 tcacggcgcg cacgggcgcc tcactccacc gtgacggact tggccaggtt cctcggctgg
     2221 tccacattgc agccccggac cttggcgatg tggtacgcga ggagctggag cggcaccgac
     2281 accacgaccg gcgagaggag ctccgcggtc cgaggcacat agaggacgtg atcggccagc
     2341 ttcccgatct cctcgtcccc ctcggtggcc agggcgatca cctcggcgcc gcgggccttg
     2401 atctcctgga tgttcgagag caccttgggg tagacgtggc acgcggtggc caccgccacc
     2461 acgggaaccc cgggctcgag tagagcgatc ggcccgtgct tcaactcccc cgccgggtag
     2521 ccttcggcgt ggatgtagga gatctccttc agcttcaggg caccctccag cgccaccggg
     2581 taacccgtgt ggcgccccac gaagagcacg tcgcgcgcgt ccgcgtaccg gcgagccagc
     2641 tcctccacgc ccgggttgag ttcgagcgtc cgctccacga gggccggcag gccctccatc
     2701 tcgtccagca cctgctggat ctcctccggg tacatcgtcc cccggacctg cgccaggtag
     2761 agcgcgacca agtggagggc caccatctgg gtggtgaagg tcttcgtggc ggcgaccccg
     2821 atctcggggc cggcgcgcgt gtagacgacg gcgtcggcct cgcgggccac cgaggatccc
     2881 acggtgttcg tgatggcggt cacccaggag cgctggtgct tcgcgtgccg gacggcctcg
     2941 agcgtgtcga tcgtctcccc cgactggctg atcgcgagcg tcagcgtgtt ccgatcgagg
     3001 actgggtcgc ggtagcggaa ctcgctggcg atctcgatct cgaccggcag gcgcgtccag
     3061 tgctcgatcg cgtacttggc gaccagcccc gcgtggaagg cggtcccgca ggccaccacg
     3121 aacaccttgt ccaccgcccg ggtgtcctcc tcgctcatcc ggacctcgtc gagcaggagc
     3181 ttcccctcgc ggatgcggcc ccgggccgta tcgaggatcg cctggggctg ctcgtagatc
     3241 tccttgagca tgaagtcctc gaagccgccc ttctcggcct gctcgatgtc ccagtcgatc
     3301 tcctctggct ccgtcgagcg ctcgttcccc tcgaggtcgg tgatgcgata cccttcggct
     3361 cgcagctcca ccatctcgcc gtcctccagc ggaacctgga acctggtcct ggccaggaag
     3421 gcgggcgcac ccgaggccag gaggttctct ccctcgccaa cccccacgat catcggcgag
     3481 gagacccgca ccccgacgac gaggtcgggc tggtcggccg ccatcaccac gagcgcgtac
     3541 gcgccctcga gctccttcac ggtggagcgc acggcggcgg ggaggtcacc ctcatagcgc
     3601 ccttcgagga ggtgggccac gacctcggtg tccgtctcgg agcggaactc gtggccctcg
     3661 gcctccagcc gctcccggtg ttcgaagaag ttctcgatga taccgttgtg gatgaccgcg
     3721 atcgcgcccg agcagtccgt ctgggggtgg gcgttctggt cggtgggagg gccgtgggtg
     3781 gcccaccgcg tgtgtcccat cccgatccgg ccgggcggct gtccctcctc ggccagcgcc
     3841 ttctcgagct cgtccagctt cccggctcgc ttcacgacac ggaggtcgtc gcccagcacc
     3901 gcaacgcccg cggagtcgta gccccggtac tcgaggcggt gaagtccgtc catgaggatc
     3961 gggagcgcct cgtcgggacc cacgtagccc acgatgccgc acatgacgcc ccatggtacc
     4021 agggggggtt ggctcggcgg accagccggg acgagccggg cgaaccggcc gtcagcccag
     4081 ggcctcgcgc acggccgcgg cgatggcctc ggcgtgacgc cgcgcctctt cctcctcctc
     4141 ggcctccacc atcacgcgca ccaccggctc cgtccccgag gcacgaacca gcacccggcc
     4201 tcgccccccc agcgcctcct cggcgcgacg gatcgcgtcc cggacctcct ccgacccctc
     4261 caggcccccc cgatcggcca cctccaccgc ctcgaggacc tgcggcagct tacgcacgac
     4321 cgaggccagc tcggcgacgg acacgccctt ggagcgggcc agggccagga accgcgccgc
     4381 cgtcagcagg ccatcgccgg tggtcgctgc gtcgaggaag atcacgtggc cggactgctc
     4441 cccgcccagg gttgcccccg tgcgccgcat ctcctcgagg acgtaccggt ccccgacctg
     4501 cgtctccacg acggagatcc cggcctcctc catcgccctc ctgaagccga ggttggccat
     4561 cacggtggtg acgaccgtgt tgcgggccag cgtcccctcc tcgcgcatgg cgagcgcgca
     4621 cgccccgagc atgtggtcgc cgtccacgat cgccccctcc gcgtcgcaga ggatggcccg
     4681 gtccgcgtcg ccgtcgtgcg ccacaccggc atcggccttg aaccggcgaa cggcctcggc
     4741 cacggtctcg ggatgggtgg ccccgcaccc cacgttgatg ttcctgccgt cgggctcgtc
     4801 cccgatggcg atcacgtcgg cgccgagcct gcgcagcagg ctcgg
//
LOCUS       DASUTZ010000232         4555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1247164, whole genome shotgun sequence.
ACCESSION   DASUTZ010000232 DASUTZ010000000
VERSION     DASUTZ010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4555
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247164"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VF097_09995"
     CDS             complement(<1..384)
                     /locus_tag="VF097_09995"
                     /inference="COORDINATES: protein motif:HMM:NF013298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX6263165.1"
                     /translation="MGYRIGVLGATGLVGEVLLSILDERGFPVDELRAFASERSAGRR
                     IGWRGEEIVLEPPAEERFAGLDLVFTAAGSDVAREWVPRIRAQGPVVIDNSSAFRQDP
                     GVPLVIPEINAHALRDHDGLIANPNC"
     gene            447..1322
                     /locus_tag="VF097_10000"
     CDS             447..1322
                     /locus_tag="VF097_10000"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEX6263166.1"
                     /translation="MSGRSFTPATVLLGVGIAATLTVGTVRALTFEPVPITGAPTPEG
                     ALRAYYEALEEEDCRGAARYVDPAFLTAEQLCERFEDTVRASGSLVGVTDREVGEETA
                     TLIAERVLGDHTDHRVVRAIRAGDLWRLAGGSSCFPVLHPTDLGTAHLEEGQAFDDYS
                     SIPPTSGPHSPVPTASGAVHREPQPLPEVVHAMEHGAVVVWVSPEAPEPMRESLLRTV
                     SSIAEDGYTSLIVTPLPGLEEPFAMTAWGSLQRCLGVSPPEIHAFVEAHYASGGEGTL
                     ACAGPAAEVPRCRDA"
     gene            1343..2287
                     /locus_tag="VF097_10005"
     CDS             1343..2287
                     /locus_tag="VF097_10005"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6263167.1"
                     /translation="MRMRPLGTSEISVIGFGAWEAGGGVWGENESDDRVIEAIRAGLD
                     AGITWIDTAEGYGSGRSEELVGRAIEGREAFVATKVAPKPEGTGHRPDEVRRACEASL
                     ERLAIDAIDLYQLHWPSDDVPVEETWGAMAELADEGLVRELGLSNFGREQIERCEAIR
                     KVDSLQPQFSILHLKHRDLIRWCGERGIAVVTYGPLAYGLLTGAIHEDTEFPDEDWRS
                     GKGAGMSYYDGLFAPGKLERSLAVVDRLRPIAKRLGITLAQLSLAWILHQPGVTSAIA
                     GSRNPDHVRQNAEAGDVALSREDLDEIDGILPLGPAFA"
     gene            complement(2284..3168)
                     /locus_tag="VF097_10010"
     CDS             complement(2284..3168)
                     /locus_tag="VF097_10010"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX6263168.1"
                     /translation="MRLVTYAREGKRRLGVLVDETVLDLPDAVGHPAFPETMEAMVRT
                     GLGGLGVAREALGRDGAVERHAVPGARLLAPLVPGSLRDFLAFEDHVREGAARRGQPF
                     PEPWHRMPVYYKGNHRSIYGPEEEVPWPRYTSELDYELEIACILGGRGRDLDEEGAAA
                     LIFGYTLMNDWSARDVQRDEMACGLGPAKAKDFATSLGPCVVTADELDPTDLWLTARV
                     DGEIWSKGNLGASRWSFPQMIAHVSQDEEVWPGDVYGSGTFGGGCGLDLDRSLRPGAV
                     VELEADLIGVLRNRVAAA"
     gene            3236..>4555
                     /locus_tag="VF097_10015"
     CDS             3236..>4555
                     /locus_tag="VF097_10015"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6263169.1"
                     /translation="MTESYQNWVGGKWVPSASGDTFESRNPANMDEVVGTFPRSSAED
                     VDAAVRAAKDAYPDWMRTPAPERADYLLRVALLLEERIEDWSELMTREMGKTLKESRG
                     DIQEGIDFLHYMAGEGRRMQGHTIPSELRAKFSMTVRHPIGVVGLITPWNFPIAIPTW
                     KLAPAIVSGCTSIFKPAEDTPLLATLLVQAFEEVGLPPGVLNLVHGFGEEAGAPMVEH
                     PDIRAISFTGSLEVGRKINERCGALMKRCSLELGSKNVMIVMPDAEMDLAVEAAAWGA
                     FATSGQRCTATSRLIVHDAAREEFTERMLKRVEGMKVGSGLDPDVELAPVINATQKER
                     ILEYIEIGKKEGAKLLTGGEELTGDGYEKGNFIAPTVFDEMTPNMRIAQEEIFGPVTG
                     IIRTDDVDDAIRMANSVDYGLSAAIYTHDITNVFKAVTQLEFGIVYVN"
BASE COUNT          693 a         1573 c         1662 g          627 t
ORIGIN      
        1 gcagttcggg ttggcgatca ggccgtcgtg gtcccggagc gcgtgggcgt tgatctcggg
       61 gatcacgagc gggacgcccg ggtcctgccg gaacgccgag gagttgtcga tcacgaccgg
      121 tccctgggcc cggatcctcg ggacccactc ccgggccacg tcggagccgg ccgcggtgaa
      181 gacgaggtcc agcccggcga atcgttcctc tgccggcggt tcgaggacga tctcctcgcc
      241 ccgccagccg atccggcgcc ccgccgagcg ctcggaggcg aaggctcgga gctcgtccac
      301 ggggaaccct cgctcgtcga ggatgctcaa gaggacctcg cccacgaggc cggtcgcgcc
      361 caagacgccg atgcggtagc ccatcgccgc caccttacca tcggggttga cgctcgcccg
      421 ggcgggcccg taccgtcccc ggcacgatga gcggccgctc cttcacccca gccaccgtcc
      481 tgctgggcgt gggcatcgcc gccacgctga cggtgggaac cgtgcgcgcg ctcaccttcg
      541 agcccgtccc gatcaccggg gcccccaccc ccgagggggc cctgcgcgcg tactacgagg
      601 cgctggagga ggaggactgc cgcggagccg cccgctatgt ggaccccgcc ttcctcaccg
      661 ccgaacagct ctgcgagcgg ttcgaggaca ccgtcagggc ctcgggatcg ctggtcggcg
      721 tgaccgaccg cgaggtgggg gaggagacgg ccacgctgat cgccgagcgc gttctcggcg
      781 accataccga ccaccgcgtg gtccgggcga tccgggcggg cgacctgtgg cggctggccg
      841 ggggcagctc gtgcttcccc gtcctgcacc ccaccgacct ggggacggcg cacctcgagg
      901 agggccaggc gttcgacgac tactcgtcga tcccgccgac ctccgggcca cactccccgg
      961 tgccgaccgc gtcgggggcc gtgcatcggg agccccagcc cctccccgag gtcgtccacg
     1021 cgatggagca cggggcggtc gtggtgtggg tctcccccga ggcgcccgag ccgatgcggg
     1081 agagcctgct ccggaccgtg agctcgatcg cggaggatgg gtacacgtcg ctgatcgtga
     1141 cgccgctgcc cgggctcgag gagccgttcg ccatgacggc ctggggctcg ctccagcggt
     1201 gtctcggggt cagccccccc gagatccacg ccttcgtgga ggcccactac gcgtcgggcg
     1261 gcgagggcac gctggcctgc gccggcccgg cggccgaggt cccccgctgt cgcgacgcct
     1321 gatggtgggg tagaacgggc ccatgcgcat gcgaccgctg ggcacgtctg agatctcggt
     1381 gatcgggttc ggcgcctggg aggcgggggg cggcgtctgg ggcgagaatg agtcggacga
     1441 ccgcgtgatc gaggcgatcc gggcgggcct ggatgccggg atcacctgga tcgatacggc
     1501 cgagggctac ggcagcggcc gctccgagga gctcgtgggg cgggcgatcg aggggcgcga
     1561 ggcgttcgtg gccacgaagg tggctccgaa gcccgagggg accgggcatc gccccgacga
     1621 ggtccgcagg gcgtgcgagg cgagcctcga gcgcttggcg atcgacgcca tcgatctcta
     1681 ccagctccac tggccgtcgg acgacgtccc ggtggaggag acgtgggggg cgatggccga
     1741 gctcgcggac gagggactcg tccgcgagct cggcctctcg aacttcggtc gggagcagat
     1801 cgagcgctgc gaggcgatcc gcaaggtgga ctcgctccag ccccagttct cgatcctcca
     1861 cctcaagcac agggacctga tccggtggtg cggggagcgg gggatcgcgg tcgtcacgta
     1921 cgggccgctg gcctacggcc tcctcaccgg ggcgatccac gaagacaccg agttccccga
     1981 cgaggactgg cggagcggga agggcgccgg gatgtcgtac tacgacggcc tgttcgctcc
     2041 ggggaagctc gagcggagcc ttgccgtcgt ggatcggctc cgcccgatcg cgaagcggct
     2101 ggggatcacg ctggcccagc tctcgctggc ctggatcctc catcagcccg gggtgacgtc
     2161 ggcgatcgcg ggcagccgca accccgacca cgtgcggcag aacgccgagg ccggcgacgt
     2221 ggcgctctcg cgggaggatc tcgacgagat cgacgggatc ctgccgctgg gcccggcctt
     2281 cgcctaggcc gcggcgaccc ggttgcgcag gacgccgatc aggtccgcct cgagctcgac
     2341 caccgccccc ggcctcagcg accggtcgag gtccagcccg cagccgcccc cgaacgtccc
     2401 cgacccgtag acgtcgcccg gccagacctc ctcgtcctgc gacacgtggg cgatcatctg
     2461 ggggaacgac cagcggctgg cccccaggtt gcccttcgac cagatctcgc cgtccacgcg
     2521 agccgtgagc cacaggtcgg tggggtcgag ctcgtcggcc gtgaccacgc acggacccag
     2581 cgaggtggcg aagtccttgg ccttggcggg ccccaggccg caggccatct cgtcacgttg
     2641 gacgtcgcgc gccgaccagt cgttcatcag ggtgtagccg aagatcaggg ccgccgcccc
     2701 ctcctcgtcg aggtctcgtc cccgtccccc caggatgcag gcgatctcga gctcgtaatc
     2761 gagctccgaa gtgtagcggg gccaggggac ctcctcctcg ggcccgtaga tcgagcggtg
     2821 gttgcccttg tagtagacgg gcatccgatg ccagggctcg gggaagggct ggccgcgccg
     2881 ggccgcgccc tcgcgcacgt ggtcctcgaa ggccaggaag tcgcggaggg agcccggcac
     2941 gagaggcgcc agcaggcgtg cgccggggac ggcgtgacgc tcgacggcac cgtcgcggcc
     3001 cagggcctca cgcgccacgc cgaggccgcc caggcccgtg cggaccatgg cctccatcgt
     3061 ctccgggaac gccgggtgac cgaccgcgtc cgggaggtcc aagaccgtct cgtcgacgag
     3121 gacgccgagg cggcgcttcc cctcccgcgc gtaggtcacg agccgcacgg gcacgatgat
     3181 agtgccagtc gcccgcgagt aggattggag cgacgcgagc caggaggacc acgagatgac
     3241 cgagagctat cagaactggg tgggcgggaa gtgggtcccg tcggcgtccg gtgacacctt
     3301 cgagtcccgc aacccggcga acatggacga ggtcgtgggg acgttcccgc gatcgagcgc
     3361 cgaggacgtg gacgccgcgg tccgggccgc gaaggacgcc tatcccgact ggatgcggac
     3421 ccccgccccc gagcgcgccg actacctcct gcgggtcgcc ctcctactcg aggagcggat
     3481 cgaggattgg tccgagctca tgacccgcga gatggggaag acgctcaagg agtcccgagg
     3541 ggacatccag gagggcatcg acttcctcca ctacatggcc ggcgaggggc ggcgaatgca
     3601 gggccacacg atcccctccg agctccgcgc caagttctcg atgacggtgc gtcacccgat
     3661 cggcgtggtg ggcctcatca ccccgtggaa cttcccgatc gcgatcccga cgtggaagct
     3721 ggcgccggcg atcgtctccg gctgcacgag catcttcaag ccggccgagg acacgccgct
     3781 gctggcgacg ctgctggtcc aggccttcga ggaggtcggg cttccgcccg gcgtcctcaa
     3841 cctggtgcac ggcttcgggg aggaggccgg cgcgccgatg gtcgagcatc cggacattcg
     3901 cgcgatctcg ttcaccggct cgctcgaggt gggacggaag atcaacgagc gatgcggcgc
     3961 gctgatgaag cgctgctcgc tcgagctcgg gtccaagaac gtgatgatcg tgatgcccga
     4021 cgccgagatg gacctcgcgg tcgaggcggc cgcgtggggc gcgttcgcca cgagcggcca
     4081 gcggtgcact gcgacgtcgc gcctcatcgt ccacgacgcc gctcgcgagg agttcaccga
     4141 gcggatgctg aagcgggtcg aggggatgaa ggtgggttcg ggcctcgatc ccgacgtcga
     4201 gctcgccccg gtgatcaacg ccacgcagaa ggagcggatc ctcgagtaca tcgagatcgg
     4261 gaagaaggag ggcgccaagc tgctgaccgg gggcgaagag ctcaccggcg acggctacga
     4321 gaagggcaat ttcatcgcgc cgacggtgtt cgacgagatg accccgaaca tgcggatcgc
     4381 gcaggaggag atcttcggcc ccgtgacggg gatcatccgc accgacgacg tggacgacgc
     4441 gatccggatg gcgaactcgg tcgactacgg gctgtcggcg gcgatctaca cgcacgacat
     4501 cacgaacgtg ttcaaagcgg tcacccagct cgagttcggg atcgtctacg tgaac
//
LOCUS       DASUTZ010000233         9819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_730058, whole genome shotgun sequence.
ACCESSION   DASUTZ010000233 DASUTZ010000000
VERSION     DASUTZ010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9819
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730058"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..392)
                     /locus_tag="VF097_10020"
     CDS             complement(3..392)
                     /locus_tag="VF097_10020"
                     /inference="COORDINATES: protein motif:HMM:NF040028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6220 domain-containing protein"
                     /protein_id="HEX6263170.1"
                     /translation="MVRALRIVYAALAWLFLAALVLQVFFAGLMLFGEPEGRALHEGT
                     GWMLHTAVILLPVTAALARAGARAIWLTLALTVVTLVQPILTFVRETPAIAALHPVNA
                     ILMTVLSLIVAQGALRLARTERIPAAS"
     gene            complement(441..1082)
                     /locus_tag="VF097_10025"
     CDS             complement(441..1082)
                     /locus_tag="VF097_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007454953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6263171.1"
                     /translation="MGKVRFQLAISLDGYVAGPDQSEEHPLGVGGMKLHEWVIELEAW
                     RRQHGMDGGAVNASTQVVEEAQANVGAYVMGRNMFGGGPGPWPSDPPWNGWWGEEPPF
                     HGPVFVLTHHPREPLQMRGGTTFTFVTDGIEAAMEQARAAAGDQDVMIGGGANVIQQC
                     LSAGMVDEFELHLVPIMLGAGERLLQNVGRLDLDQVRVIEAPGVTHLKYLVRR"
     gene            1157..1600
                     /locus_tag="VF097_10030"
     CDS             1157..1600
                     /locus_tag="VF097_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013476057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEX6263172.1"
                     /translation="MGNSYVKLGIALFLSLIVMFVLTMSMIVTIDHFYFNWSNLWMAI
                     IMVAPMGIIMLAVMGSMFENVRLNLALLAAFVIILVGAFFLGRAETFVGNEGFLRSMI
                     PHHSRAILVCEEASITDPEIIELCDQIVEAQREEIRQMEQILERY"
     gene            complement(1597..1929)
                     /locus_tag="VF097_10035"
     CDS             complement(1597..1929)
                     /locus_tag="VF097_10035"
                     /inference="COORDINATES: protein motif:HMM:NF042672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6624 domain-containing protein"
                     /protein_id="HEX6263173.1"
                     /translation="MRCRLATRGFSLLAICLLGLASCSDEAQHDPELRVDPVDVAYLE
                     DRVAVNRGRPQTYGSQIRCMEGRPEPATPIEDAGNVDARRKEVGLDPLDDYYAELEEA
                     CAAEAAGT"
     gene            2555..3277
                     /locus_tag="VF097_10040"
     CDS             2555..3277
                     /locus_tag="VF097_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263174.1"
                     /translation="MRDRSSLRLLALLVGVLVASGTQSVSAHRSELSVEIVSQGSTLT
                     PNGSSMTFDLETRCDPKWTIVEARVRVEQSQAWGETSFTPRCRRLTYGVRVTVPAASG
                     TFETGTAQANAMLIVRQSKTKESSDSASLRVRPSVSLVLGDGGALEDGGAAVSVHATV
                     TCPRHATGLGGEVRVYDGQAVGTGSFGPTPCDGDPHTVSVRVPASEGTFRAGPAEAFA
                     FVGIEEGGDVFSGIDLKTIEVA"
     gene            3398..4366
                     /locus_tag="VF097_10045"
     CDS             3398..4366
                     /locus_tag="VF097_10045"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX6263175.1"
                     /translation="MGHGRGRRSHHRAGPRARRGTSLTALAVILLIALPPEGSAGRTP
                     ACGGELPTIQGTAGDDRLVGTPGLDVIAAGDGDDVVLGLGGADRICGGRGDDRLRGGE
                     DRDLLFGGPGDDVIRGGPGGDGLQGGAARDRLLGGRGADVALGGPHGDRIAGGPGRDT
                     AVGGLIGGGGPDLIRGGPGSDRLTDGPGRDRLLGGRGPDMLIPSRHAQEDLLDGQAGR
                     DLVFYFHVPRGVRVHLASGLAEGYGNDRLSRIEDAEGSRFDDVLIGDGGPNELLGVEG
                     DDRILGRGGPDLLDGFAGTDVLRGGPGRDRCIDGERHRSCEAIWPP"
     gene            complement(4886..5047)
                     /locus_tag="VF097_10050"
     CDS             complement(4886..5047)
                     /locus_tag="VF097_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263176.1"
                     /translation="MEIELELKLSSLGALLVGFATGLALGFLVGSGGGRKTADRLRSI
                     RGEAEAALP"
     gene            5112..5687
                     /locus_tag="VF097_10055"
     CDS             5112..5687
                     /locus_tag="VF097_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263177.1"
                     /translation="MTLALFIPGRTARGSRVSSDWSAVEMADSVEPTEEVRHMRSETK
                     IGLPELDGRTRQPEVGRSHGYLFGGLSLGAIWLSVILMGVFAPDLVTGAEQDRFPLAV
                     VVGLLAGLAATRSVVRAFAHMGSATRTVWIWYAMAVGLLWLAVALAAILLPVSVTGAD
                     PTRLPMAVVIAPIGGTILTGAITEVFISIRR"
     gene            5733..6587
                     /locus_tag="VF097_10060"
     CDS             5733..6587
                     /locus_tag="VF097_10060"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6263178.1"
                     /translation="MASITRKNLREPAEVVTFPGVTQGVVELGDLTVGRFVAQPGWRW
                     SEHFRPEVGGEWCRARHVGVVISGRARVISQDGTELELGPDDAYDVQPGHDAYVLGDE
                     PLVQIEWQGLRTFAGSRHGPPGRSLAALLFTDLVDSTSTAAALGDADWRELLARHYQA
                     VRSELDRFGGREVKTTGDGILAVFEGPAAALRCAAAIRSKAPSHGLRVRAGVHVGEVD
                     VVGTSVEGLAVHEAARIMAAADPQEILTSEITRTLLASSDIELEDRGSRTLKGLSGER
                     RLFAYVAD"
     gene            complement(6572..7636)
                     /locus_tag="VF097_10065"
     CDS             complement(6572..7636)
                     /locus_tag="VF097_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 domain-containing protein"
                     /protein_id="HEX6263179.1"
                     /translation="MTTESRRVLAERCRVQAGWCERLGSPLYAVLLRRAADDVEAGGP
                     TWSVLRGREEAPEEFYVALRLMGSVHRLVLQGRAPALAAHYPSAGGSPGEGAWDEFRA
                     VVDANIEELRGLLDLPPQTNEVGRSAALLGGFLLVARESGLPLRLLELGASAGLNLLW
                     DRYRYESADWSWGEPTSPVRFLGVFEDATPEGGDVAVGERRGCDLAPIDPTTEEGRLT
                     LLSYVWPDQIERIERLRGALSVAREVAVRVERADAASWLGGELRRQHPGEATVVFHSV
                     VLGYLEERSRREVVAMLEEAGEAATADAPIAWLSLERAGDVFEVRLRSWPGGRDRLLA
                     LTAPHGPPVRWREPDDQSAT"
     gene            complement(7700..8116)
                     /locus_tag="VF097_10070"
     CDS             complement(7700..8116)
                     /locus_tag="VF097_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018777848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX6263180.1"
                     /translation="MRTEQSIEIDAPIDEVWAALTTADQIEQWFLGVRTETDWRPGSP
                     IVHRGEYQGKPYEDAGTILEFEPPNRLVHTHWSPASGLPNVPENHEVVRWELGERDGG
                     TVLTIKESNLPSEEAKETSERTWRVVLENLKELLEG"
     gene            complement(8118..8681)
                     /locus_tag="VF097_10075"
     CDS             complement(8118..8681)
                     /locus_tag="VF097_10075"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEX6263181.1"
                     /translation="MEALIVIDAQNEFSAEGRRPVPNHAEALEAIRRRVDEARREGRP
                     IAWIRHHNPPDEGPAFQPGTWGAELSPGLGPEPGHGPETELQKDVYGAFTGTDLESWL
                     EDVGADAVLLVGFYAHVCLSTTAREGLMRGLAVSLDPEGSGSCDIEHDVLGTQSAEEV
                     RRSALLQLAQMGARIAPSRTQEAAASG"
     gene            complement(8943..>9819)
                     /locus_tag="VF097_10080"
     CDS             complement(8943..>9819)
                     /locus_tag="VF097_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6263182.1"
                     /translation="DMGLEPSVVPKMGLAEKCRHCGRCVFGCPYDIKWDTRAFLDQAV
                     ARGAELVTGARVDRLVIEGGCARGVLVGRRPGRRLYEADLIVIAAGGFGTPAILERSG
                     IASEPGLFVDPVLTIAARIPAAWQCNEIEMPFVVQRPHYLLSPYFDWISALFNPAWRF
                     PVPDMVGVMIKLGDEGTGSVSPRGRVDKRLTALDRTRLEEAGGIAAEILGRFGADPET
                     VFTGTVNAGHPGGALPLSEATATGFHDERLPENVYVADASLLPRSLGGPPILTIIAMA
                     KRVARLCRDRAFAGV"
BASE COUNT         1430 a         3518 c         3442 g         1429 t
ORIGIN      
        1 ggtcagctcg cggcggggat ccgctccgtc cgcgccagcc gaagggcccc ctgtgccacg
       61 atcaacgaca gcacggtcat caggatcgcg ttgaccgggt ggagcgcggc gatcgcgggc
      121 gtctcgcgca cgaaggtgag gatcggctgg accagcgtga ccaccgtcag ggcgagggtg
      181 agccagatgg cgcgcgctcc ggcccgcgcc agcgcggcgg tcacgggcag gaggatcacg
      241 gccgtgtgga gcatccatcc cgtgccctcg tggagggcgc gaccctcggg ctcgccgaag
      301 agcatcaggc ccgcgaagaa cacctggagg acgagggcgg ccaggaacag ccaggccagc
      361 gccgcgtaga cgatgcggag ggctctcacc atgtcggcgg tgaggatagc cgaagtcatc
      421 ggctcgaggc gcagctccgt tcagcggcgg acgaggtact tgaggtgcgt gacgcccggc
      481 gcctcgatca ctcgcacctg gtcgagatcg agacgaccga cgttctgcag gagtcgctcc
      541 cccgccccca gcatgatcgg gaccaggtgg agctcgaact cgtccaccat gccagccgag
      601 aggcactgct ggatcacgtt cgcgcccccg ccgatcatca cgtcctggtc gccggcggcg
      661 gctctcgcct gctccatcgc cgcctcgatc ccgtcggtga cgaaggtgaa cgtcgtgccg
      721 ccccgcatct gcagcggctc ccgtgggtgg tgggtcagca cgaagaccgg gccgtggaag
      781 ggcggctcct cgccccacca tccgttccag ggagggtccg acggccaggg gccggggccg
      841 ccgccgaaca tgttccgtcc catcacgtac gcgcccacgt tcgcctgggc ctcctcgacg
      901 acctgggtgc tggcattgac ggcgccgccg tccatcccgt gctgcctgcg ccacgcctcg
      961 agctcgatca cccactcgtg gagcttcatc cccccgaccc cgagcggatg ctcctcgctc
     1021 tggtccgggc cggcgacgta gccgtcgagc gagatcgcga gctggaacct gaccttcccc
     1081 atcgtgcgaa ctcctcggac gttcgacggc cccgccatcg ggtatggact cgtcactcca
     1141 caaccaggag gtaccgatgg gaaattccta tgtgaagctg gggatcgcgc tgttcctgag
     1201 cctgatcgtg atgttcgtcc tgacgatgtc gatgatcgtc acgatcgatc acttctactt
     1261 caactggagc aacctctgga tggcgatcat catggtggct ccgatgggga tcatcatgct
     1321 ggcggtgatg ggcagcatgt tcgagaacgt ccggctcaac ctcgccctcc tcgccgcctt
     1381 cgtcatcatc ctcgtcgggg cgttcttcct cgggcgcgcg gagaccttcg tgggcaacga
     1441 gggattcctc cgctcgatga tcccccacca ctcccgcgcc atcctcgtct gcgaggaagc
     1501 ctcgatcacc gacccagaga tcatcgagct ctgcgatcag atcgtggagg cgcagcgcga
     1561 ggagatccgc cagatggagc agatccttga gcggtactag gtcccggccg cttcggccgc
     1621 gcaggcctcc tcgagctccg cgtagtagtc atcgagagga tcgaggccca cctccttgcg
     1681 ccgcgcgtcc acgttgccgg catcctcaat cggcgtggcc ggctcggggc gtccctccat
     1741 gcatcggatc tgcgagccgt aggtctgggg cctccctcgg ttcacggcca cccggtcctc
     1801 cagatacgcc acgtccacgg gatccacccg gagctcgggg tcgtgctggg cctcgtcgga
     1861 gcaggaggca agcccgagca ggcagatcgc cagcagggag aaccctcggg tggcgaggcg
     1921 gcatcgcacg aagcaaggcc tagctccctc cccgtctggc gcaccatgtc cgcaaggtcg
     1981 cctcaccgtc ctcgggcacg gttggcacgg gcatccacca tgaggtcctt actccgcgag
     2041 cggctggccg tcggtagccg tcacgacctt ccagggcggt ggccgagacc ttctcgggga
     2101 gggcgagggg cgcccgcccg gcgctcgtgg agggcgccgc gggcgcggtc tgggcgccgg
     2161 gcgggcgccc ccgcgtcgtc ttcgccttca ccgtgtcgga cgcgagggtc accgccatcg
     2221 agatgctcgc cgacgccgag cgactgcggg acctccacct ggagccgtcc ccgacaccga
     2281 gggcacgggc ggggagttct ggatcgtcgg gagaggcttc cccgtcgagg atcctgagac
     2341 ccgggccgcg gcgagggatg ccgcgaccta cgccccctcg gatcgctacg tcctcttcga
     2401 gctgcggatc tcggaggcgc gctgcaacgc gcacggggac gtccccctcc cagcccgacg
     2461 ccgctggccc agcccctagt caagcgcccc ggttcgcaat cccctgggga gcctcggcca
     2521 gtagtacctg cgacgacgca aggaggtgtt cgtgatgaga gaccggtcct ccctccggct
     2581 cctcgccctg ctggtcgggg tgctcgtggc gagtggtacg cagtcggtga gcgcccaccg
     2641 gagcgagctg tcggtcgaga tcgtgtcgca gggatcgaca ctgaccccca acggctcgtc
     2701 catgaccttc gatctcgaga ccaggtgcga tcccaagtgg acgatcgtgg aggcccgtgt
     2761 gcgcgtggaa cagtcgcagg cctggggtga gacctcgttc accccccgct gccggcggct
     2821 gacctacggg gttcgggtca cggtcccggc cgcctcggga accttcgaga cgggcaccgc
     2881 ccaggcgaac gccatgctca tcgtccggca gagcaagacg aaggagagca gcgactcggc
     2941 gtctctccgc gtccggccca gcgtgtcgct cgtgctcggc gacggcggag cgctcgagga
     3001 tggcggcgcc gcggtgagcg tccacgccac ggtgacgtgc cccaggcacg ccaccgggct
     3061 cggcggagag gtgagggtgt acgacggcca ggccgtcggc accggctcct tcggtccgac
     3121 gccgtgcgac ggggatcccc acaccgtgtc ggtgagggtt ccggcgtccg agggcacgtt
     3181 ccgggcgggt ccggcggaag ccttcgcgtt cgtcgggatc gaggaaggcg gcgacgtctt
     3241 cagcgggatc gacctcaaga cgatcgaggt cgcctagccg ggctggcctc ccgggaggcg
     3301 ccaggcccgc cgggggttgc caggagcgca gatcgggagg acgatcgccc cgtcgcggca
     3361 gcgcgcgccg cgcagacccg gagagcgggg tcacagagtg ggtcacggca ggggacgtcg
     3421 gagtcatcac cgggcgggac cccgcgcgcg tcgcggcacg tcgctcaccg ctctcgccgt
     3481 catcctgctc atcgccctcc cgcccgaagg atcggccggc cggacgccgg cctgtggcgg
     3541 agagctcccc acgatccaag gaacggccgg cgacgatcgc ttggtcggca cacctggcct
     3601 cgacgtcatc gccgcggggg atggggacga cgtcgtcctc ggcctgggcg gcgcggacag
     3661 gatctgcgga gggcgcggcg acgatcgcct tcgagggggc gaggatcgcg atctcctgtt
     3721 cggagggccc ggcgacgacg tgatccgggg cgggccgggg ggcgacggac ttcagggagg
     3781 cgcggcacgc gatcggctcc tgggtggccg cggagccgac gtcgcgctcg gcggccctca
     3841 cggcgatcgg atcgccggcg gtccggggag ggatacggcg gtcggaggcc tgatcggagg
     3901 cggcgggccc gacctgatcc gaggaggacc gggcagcgat cgcctcacgg acggacccgg
     3961 gagggatcgc ctcctgggcg gccgcggccc cgacatgctg atcccctccc gacacgctca
     4021 agaggacctg ttggacggcc aggcggggcg ggatctcgtc ttctacttcc acgtcccgcg
     4081 aggggttcgc gtgcacctgg cttccgggct cgccgagggc tacgggaacg atcggctgag
     4141 caggatcgag gacgccgagg gcagcaggtt cgacgacgtg ctgatcgggg acggaggacc
     4201 caacgagctc ctgggcgttg agggcgacga ccggatcctc ggccggggtg ggcccgatct
     4261 gctcgacggg ttcgcgggaa cggacgtgct ccggggcggg ccgggtcgcg atcgatgcat
     4321 cgacggcgag cgccaccgga gctgcgaagc gatctggccc ccctagccgc gatccccgct
     4381 catcgcttcg gctgccagag gccgacctcc gcgcccctgc cgggacgtcg aggatcgcgt
     4441 accagcccgc cggactccag tcggttccag cggaaccgtc gccccgaggg gccgagctcg
     4501 ctcgatcgcg tcatcgacgt cggacacctc gacgaaagcg agccagaaga cccgagcccg
     4561 ctccaggtcg ttcgtttgca gctccacctt ctcgatgcgg ccgtccgcga gctgggccca
     4621 gggcagagag gccgtcgcgg agtcctggcc gatgcccacc tggaccctcc accagcgcga
     4681 acggccagcc ggccgtggcc gccctaccgg ctcggcgagg acgcgcttcc tgccgctagt
     4741 gctcgacgtc ctcaccctgc ccgacgagct gatcagccac gtcacggcgt ttcggagccc
     4801 agagctcgtc gaggcgttcg ggctcccggc cgaggtcgcc gcgtagcgac gagcctcagc
     4861 gaagcgaagc gtgggacgct ctcggctacg ggagggccgc ctcggcctcg ccccggatgc
     4921 tccgcaaccg atcggcggtc ttccggcctc caccactgcc gaccaggaac cccagggcca
     4981 gaccagtcgc gaagccgacc agcagggccc cgagcgacga gagcttcagc tccagctcga
     5041 tctccatctc gcctccgcga ttacccggcc ggagggtcgc tcaacccgcg gacgcccgac
     5101 ccgaccgttt cgtgaccctg gcgctcttca tcccgggccg gacggcccgc ggatcgaggg
     5161 tcagcagcga ctggtcggcg gtggagatgg cggatagcgt cgagccgacc gaggaggtga
     5221 ggcacatgcg cagcgagacg aagatcggcc ttcccgagct ggatgggcga acgaggcagc
     5281 ccgaggtggg gcgaagtcac ggatacctct tcggcgggct gtcgctcggt gccatctggc
     5341 tctcggtgat cctgatgggc gtcttcgcgc cggatctcgt gacgggcgct gagcaggatc
     5401 gcttcccgct cgccgtcgtg gtcgggctgc tggcagggct ggcggccacc agatccgtgg
     5461 ttcgggcgtt cgcacacatg ggatcggcga cccggaccgt atggatctgg tacgcgatgg
     5521 ccgtgggcct gctctggctc gcggtggcgt tggcagcgat cctcctgccg gtgagcgtaa
     5581 cgggggccga cccgaccagg cttccgatgg cggtggtgat cgctccgatc ggcggcacca
     5641 tcctcacggg cgccatcacg gaggtgttca tctcgatccg acgataagcg attaggctgg
     5701 cgcgaagcag gccatgaggc catcatcggc ccatggcttc gatcacgagg aagaacctcc
     5761 gtgagccggc cgaggtggtc accttccccg gcgtcacgca aggcgtcgtc gagctcgggg
     5821 acctcaccgt cggtcggttc gtcgcacagc cgggatggcg ctggtccgag cacttccgcc
     5881 ccgaggtcgg gggtgagtgg tgccgggcca ggcacgtcgg cgtcgtgata tcagggcggg
     5941 cacgagtgat ctcccaggac gggacggagc tcgagttggg accggatgac gcctacgacg
     6001 ttcagcccgg gcacgacgcg tacgtgttgg gcgacgagcc gctcgtgcag atcgagtggc
     6061 aggggctccg caccttcgct ggatcccgcc acggaccgcc cggccgcagc ctcgccgccc
     6121 tgctcttcac agacctcgtc gattccacct cgaccgcggc ggcgctcggg gatgccgact
     6181 ggcgggagct gctcgcccgc cactaccagg ccgtgcgaag cgagctcgac cggttcgggg
     6241 gccgggaggt caagaccacc ggggacggga tcctcgccgt gttcgaaggc ccggcggccg
     6301 ccctccgctg cgccgccgcg atcagatcga aggcgccctc gcacggcctg cgggttcgag
     6361 cgggcgtcca cgtcggggag gtcgacgtgg tgggcacgtc ggtggagggc ctggccgtcc
     6421 acgaggccgc caggatcatg gcggcggccg acccgcagga gatcctgacg tccgagatca
     6481 ccaggaccct gctcgcgtcc tcggacatcg agctcgagga tcgaggatcc cgcaccttga
     6541 agggtctctc tggggagcga cggctgttcg cctacgtcgc cgactgatcg tccggctccc
     6601 gccaccggac gggcggcccg tggggcgcgg tgagggcgag gaggcgatcg cgcccgccgg
     6661 gccaggagcg cagccgcacc tcgaagacgt cgccggcgcg ctcgagcgac agccaggcga
     6721 tcggggcgtc cgcggtcgcc gcctcgcccg cctcctctag catcgcgacc acctcacgcc
     6781 gagagcgctc ctcgagatag ccgagcacca ccgagtggaa gacgaccgtc gcctcccccg
     6841 ggtgctgtcg gcgaagctcc ccgccgagcc agctcgccgc gtcggcccgc tcgacccgaa
     6901 cggcgacctc ccgggcgacg ctgagagccc cccgcagccg ctcgatcctc tcgatctgat
     6961 ccggccagac gtacgagagg agcgtcagtc ggccctcctc ggtcgtggga tcgatcgggg
     7021 cgaggtcgca tccgcggcgc tcccccaccg cgacgtcccc gccttccggc gtcgcatcct
     7081 cgaacacgcc caggaaccgt accggtgacg tcggctcccc ccaggaccag tccgccgact
     7141 cgtagcggta ccgatcccag aggaggttga gcccggcgct ggccccgagc tcgaggagcc
     7201 ggagcggcag cccggactcg cgcgcaacga ggaggaaccc tcccaacagc gccgccgacc
     7261 gaccgacctc gttcgtctga ggcgggaggt cgagcagccc tcggagctcc tcgatgttcg
     7321 cgtccaccac cgcccggaac tcgtcccaag ccccctcgcc gggggagccg cccgccgagg
     7381 ggtagtgcgc ggccagcgcc ggcgcgcggc cctggagcac caagcggtgg accgacccca
     7441 tcagacggag ggcgacgtag aactcctcgg gcgcctcttc gcgcccccgg agcacgctcc
     7501 aggtggggcc cccggcctcg acgtcgtccg cggcccgacg tagcagcacc gcgtagagcg
     7561 gtgatccgag gcgctcgcac cagcccgcct gcacccggca gcgctcggcc aggacgcgac
     7621 ggctctcggt ggtcacccgg gaagtatggc cggggacgcg tcgggtgcga agctcaaggc
     7681 cgggagtcgg ggaccgggat cagccctcca agagctcctt gaggttctcg aggaccacgc
     7741 gccatgtcct ctccgacgtc tccttcgcct cctcggaggg gaggttagac tccttgatcg
     7801 tcaggaccgt gccgccgtcg cgctcgccga gctcccagcg gaccacttcg tggttctcgg
     7861 gcacgttggg gagtccggag gccgggctcc agtgggtatg cacgaggcgg ttcggcggct
     7921 cgaactccag gatcgtcccg gcgtcttcgt agggcttccc ctggtattcg ccgcggtgaa
     7981 cgatcgggct gcccggccgc caatccgtct ccgtccggac gcccaggaac cactgctcga
     8041 tctggtcggc ggtcgtcagc gccgcccaga cctcgtcgat cggcgcgtcg atctcgatcg
     8101 attgctcggt ccgcacgtca cccactcgcg gcggcctctt gcgttcgtga gggggcgatc
     8161 cgagcgccca tctgcgccag ctggagcagg gccgagcggc ggacctcctc ggcgctctgc
     8221 gtgccgagga cgtcgtgttc gatgtcgcac gacccgctcc cctccgggtc gagggacacg
     8281 gcgaggcccc gcatcagccc ctcccgagct gtggtcgaca ggcacacgtg cgcgtagaag
     8341 cccacgagca ggacggcgtc cgcgcccacg tcctcaagcc acgactccag gtcggttccc
     8401 gtgaaggccc cgtagacgtc cttctggagc tcggtctcgg gtccgtgccc cggctccggc
     8461 cccagcccgg gggacagctc cgcgccccag gtccccggct ggaacgccgg cccctcgtcg
     8521 ggggggttgt ggtgacggat ccaggcgatc ggacggccct ccctccgcgc ctcgtccacc
     8581 cggcgacgga tcgcttcgag cgcctccgcg tgattgggga cgggtcgcct gccttcggct
     8641 gagaactcgt tctgtgcgtc gatgacgatc agcgcctcca tccgccctcc tccctggtga
     8701 gacatccccg agcttccgtc cctctcaccc ccgaaggggg agggtcgcgc agatacggaa
     8761 gcgaacctcg tgctcctcca agagcgtagc ccccctcacc ctgcacggca cagggatggg
     8821 tcctccctcc tgtcccgggg catcgccccc tcgggcgtca tcgaagctgc ccctcacact
     8881 agacggcggg aaagtcacga gccggttccg tgccggtgag gtcgtggctg catccttccc
     8941 cctcagacgc cggcgaacgc ccggtcgcgg cacagcctcg ccacgcgctt cgccatggcg
     9001 atgatcgtga ggatgggggg acccccgagc gagcgcggga gcagcgacgc gtccgcgacg
     9061 tacacgttct ctgggagccg ctcgtcgtgg aagccggtcg ccgtggcctc ggacagcggg
     9121 agcgctccgc ccggatgtcc ggcgttgacc gtgccggtga acacggtctc gggatccgcc
     9181 ccgaagcgcc cgaggatctc ggccgcgatc ccccctgcct cctcgagccg ggttcggtcc
     9241 agcgcggtga ggcgcttgtc gacgcgaccg cgcggcgaga ccgagccggt tccttcgtcg
     9301 cccagcttga tcatcacgcc caccatgtcg ggcaccggga agcgccaggc ggggttgaag
     9361 agcgcactga tccagtcgaa gtacggcgac aggaggtagt ggggccgctg gaccacgaac
     9421 ggcatctcga tctcgttgca ctgccaggcc gcggggatcc gcgccgcgat ggtcaggacc
     9481 ggatcgacga acagaccggg ctcgctggcg atgccggagc gctcgaggat ggccggggtc
     9541 ccgaagcccc cggcggcgat cacgatgagg tcggcctcgt agagccgacg ccccggccgc
     9601 cggcccacca ggacgcccct ggcgcagcct ccctcgatca cgagcctgtc gacccgggcg
     9661 ccagtcacga gctcggcccc gcgggccacc gcctggtcca ggaaggcccg ggtgtcccac
     9721 ttgatgtcgt aggggcagcc gaacacgcac cgcccgcagt gccggcactt ctcggccagc
     9781 cccatcttgg gcacgacgga cggctccagg cccatgtcg
//
LOCUS       DASUTZ010000234         2306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1377214, whole genome shotgun sequence.
ACCESSION   DASUTZ010000234 DASUTZ010000000
VERSION     DASUTZ010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377214"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(435..>2306)
                     /locus_tag="VF097_10085"
     CDS             complement(435..>2306)
                     /locus_tag="VF097_10085"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6263183.1"
                     /translation="ASVVDPALVPSAIAHALKLPEVAGRPIVDSRKDHLADKELLLLL
                     DNFEQVAQAGPVLEDLLGSASGLSALATSRRPLSVRGEQEYLVPPLDPPDTERLPDLP
                     VLREIDGVRLFLERAASVAPRFEMTEENAPAIAAIVARLEGLPLAVELAATQTKLLSP
                     EEIAARLDERLTAVGTGARTAPQRQRTLRDAIAWSYQLLGEPERRLFTRLSVFSGGWT
                     VKAAEAVCEPDSLGDLLELFGSLVDHSLVRRLKTPEAESRYSMLETIREFGREQLRAG
                     GEEEAIARRHGEHFLGLAVEAEPHLESDLAWLARCEAEHDNMRAALRWAIAAGEPDLS
                     QGAAGALWRFWRTRGYLTEARLWFDEVLALPSGDRPTAARAKALTGAGGIAWWRGEVP
                     ESRAAYEEALAIERELGDRRRIAEALYNLAFPLSIQEGLEAAVAVAEESLELFREVGD
                     ESGVARALGMLSISDFMAGNWDAVVARTEDAVAIWRRTGDRLRLLNDVLALGFVYLRV
                     GRPGEARAAALEALDLAEEVGSPMEIGGALLALAVQANEEGRHLDAMRLSGAAESLRE
                     QVGSGGPPTEIMQVIVGDPAAEARAHLDQDEAERVLEEGRAMTLEEAVAEARRSASG"
BASE COUNT          357 a          859 c          775 g          315 t
ORIGIN      
        1 cgtaccaggc ccagcgctcc ccccgccggt agcccgtcgc gacgacggtg gtgaagagca
       61 cgaagaatcc caacccgaag atcgccatga ccctcacgaa gcccgtgatc gactccgcaa
      121 ccgcggggct ctcggcgacc aactgctccc aggtcatgcc gaggctgcta ttcgcaaact
      181 gaagccccgc cgccaggggg gtcccggttg cgagatggcc gatgctcccg aacagcatcg
      241 gcaagcccgc gatcaggagc aggatccagg cgtacctctc gtgcgtcctc ggcgatgtcg
      301 ttcgtgaagg cgtctcagtc atgctgggac ctcctaggtt gacggggcta tctctgcaag
      361 atgagcgtct cgccttcctc tcgagaggga gcgtgctcga tgaccggccg agggaccagg
      421 gggtggaacg ggcatcagcc ggatgccgaa cggcgtgcct cggccacggc ttcctccaag
      481 gtcatcgccc ggccctcctc caggacgcgc tccgcctcgt cctggtccag atgagcccgc
      541 gcctcggccg ccggatctcc cacgatgacc tgcatgatct cggtgggagg cccgccgctc
      601 cccacctgct cgcggaggga ctccgcggcg cccgagagcc gcatagcatc caggtgcctg
      661 ccctcctcgt tggcctgaac cgcaagcgcc aggagggctc ccccgatctc catggggctc
      721 cccacctcct cggccaggtc cagggcctcc agcgcggccg ctcgggcctc gccgggccgc
      781 cccacccgca gatagacgaa ccccagggcc agcacatcgt tcagcaggcg caaccggtcg
      841 cccgtccgcc gccagatcgc gaccgcgtcc tcggtccggg cgacgacggc gtcccagttc
      901 ccggccatga agtcgctgat cgagagcatc ccgagggccc gggcgacccc cgactcgtct
      961 ccgacctccc ggaacagctc gaggctctcc tccgccacgg ccacggcggc ctccagaccc
     1021 tcctggatcg acagggggaa cgcgaggttg tagagggcct ccgcgatgcg tcgccgatcg
     1081 ccaagctcgc gctcgatggc cagggcctcc tcgtaggcgg cacggctctc cggtacctct
     1141 ccacgccacc aagcgatccc ccccgccccg gtgagcgcct tcgcccgcgc cgcggtcgga
     1201 cggtcgcccg agggcagcgc gaggacctcg tcgaaccaga ggcgggcctc agtcaggtat
     1261 ccgcgcgtcc tccagaagcg ccaaagcgcg cccgccgctc cctgggacag gtccggctcg
     1321 cctgcagcga tcgcccaccg aagggccgcc cgcatgttgt cgtgctccgc ctcgcaccgg
     1381 gcgagccagg cgagatcgct ctcaaggtgg ggctcggcct cgacggccag ccccaggaag
     1441 tgctctccgt gacggcgagc gatcgcctcc tcctcgcccc ccgcgcggag ctgctcccgg
     1501 ccgaactcgc ggatcgtttc gagcatcgag tagcgcgatt cggcctcggg cgtcttgagg
     1561 cggcggacca gtgaatgatc gaccagggag ccgaagagct cgagcagatc cccgagggag
     1621 tcgggctcgc acaccgcctc ggccgccttc accgtccacc cgccagagaa gaccgataga
     1681 cgcgtgaaga gtcgacgctc cggctccccg aggagctgat agctccacgc gatcgcatcc
     1741 cggagggtcc gctgccgctg aggggcggtc cgggcgccgg tccccaccgc ggtgaggcgc
     1801 tcgtccagcc gggcagcgat ctcctcaggc gagagaagct tggtctgggt ggccgccagt
     1861 tcaacggcga ggggaagccc ctccagccgc gccacgatcg cggcgatcgc gggggcgttc
     1921 tcctcggtca tctcgaaccg aggcgccacg gaggccgccc gctccaggaa caggcggaca
     1981 ccgtcgatct cgcggagcac cggcaggtcg gggaggcgct ccgtgtcggg cgggtcgagg
     2041 ggcggcacga ggtactcctg ttcgcccctg accgagaggg gcctccggga cgtggccagc
     2101 gcgctgagcc ccgaagccga tcccaggaga tcctcgagca cgggacccgc ctgggccacc
     2161 tgctcgaagt tgtcgagcag gaggagcagc tccttgtcgg ccaggtggtc cttccgggag
     2221 tcgacgatgg gccggccggc cacctcggga agcttgaggg cgtgagcgat ggctgagggg
     2281 accagggccg ggtccaccac ggaggc
//
LOCUS       DASUTZ010000235         6770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1508546, whole genome shotgun sequence.
ACCESSION   DASUTZ010000235 DASUTZ010000000
VERSION     DASUTZ010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6770
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508546"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..1341
                     /locus_tag="VF097_10090"
     CDS             55..1341
                     /locus_tag="VF097_10090"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX6263184.1"
                     /translation="MRRIKFLGILVVLVLVAAACGDDGDDGATTGGGDNGGTTTTGEG
                     PLAEYGESTDADPALVEKALGPVEDVPDIVLASIARADQNPDDATIEKALECWNNVEC
                     ETGSGGELIMGVADGGRGNVWRQVTHMEAILQALTYPEIGTIVSRDAQWAEDPAVAEA
                     DIRFLIQRGADFIIGYPDQGTNIAGAILEAEAAGIPYIPYSAGWVGLPGQEGALVPGE
                     DYLTIVGEDLCELGESFADVLNEEVGSGTIGVMGGTPGNALSAGWQQCLRPALNDSIE
                     LVGPPADTYWVNDFALQTVLGWLSTDPDIKGYAYEYADGMYTALDAYEQLDVPVEDIT
                     IAVRTDEQTLFCDWAERNEPSYKIYFSAGGNFQSRIAVTAAMMHLKGAEIPAEIVVPH
                     VMREVTAEDCDPNRVEETVSNTSLVPDDVLAQMYEG"
     gene            1338..2927
                     /locus_tag="VF097_10095"
     CDS             1338..2927
                     /locus_tag="VF097_10095"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX6263185.1"
                     /translation="MSGSGSEGGSTPGDVVLELRGVYRQFGAVRALTDVSFDCRAGEI
                     HAVVGENGSGKSTLLGIASGFVDPDRGTIRIGRERLRRDSPAEARKLGLAMAYQDTSL
                     ILDQPVKNNLYLAAPPDKRPPFYRRKRWARRLLAELDLDLELFPDMPAGALSLSERQL
                     FEVAKALVTDPLVLLLDEPTTALGPHEVEALHRTLAACRERGVGVVYVSHRLPEVLEV
                     ADRVTVLRDGRSQGTFDAGAVAEPELVDLIVGRPFEAAFPQASPGAGEPRDVLVVDGL
                     QGQSFGPVSFTLESGEIVGIAGAEGNGQPQLFECLSGRQAPKAGRVVCDGKELMLIST
                     HEAVSAGLMVLPGDRKHEALMPVLGVRVNSTIQSLRRFGVLGILRRRKERRSVEDLIG
                     RLEIRTPSLEQPVQFLSGGNQQKVAVSRSFLKEPSVILAYEPTQGVDVGSRFDIYEAL
                     RSRSDAGTALLVKSSDPLELTGLCDRVLVMSRGQIVEEIPGDELTEHRIVEAIVRGPG
                     MSRAARSPLGVAMPKASRSGS"
     gene            2924..5377
                     /locus_tag="VF097_10100"
     CDS             2924..5377
                     /locus_tag="VF097_10100"
                     /inference="COORDINATES: protein motif:HMM:NF006056.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6263186.1"
                     /translation="MSDRIDAARRAVRKVLRDARDVNKWRKIVRFRLWMPVALQVLLL
                     IAVAWYTTTRFPGFINTTNVTNILILAAPLAVVAIGQTQALLVGYLDLSVGAMVTFGV
                     VIASFLIGPDAGAAQIALGAGVIFLAGLGLGLVNMVLVLGFKIPSIIATLATLSILNG
                     ISLTLRDTPGGIINRDFTASLGTTLGPFPISFILIVLAAGALDYWLHASGSGLQLRST
                     GFDERSAKRSGVRTKWVQGRALVLSALFAALASFLVMTRSGVGNAAIGDSYVLNSITA
                     AVLGGAALSGGRATFVGTLVASVLLALIISALPFLGLGAEHGLMIIGVLVLLAIVLFQ
                     AGDIKELVKRNYKRGRRLVIGSRSPKRAEIPDFYPSGPDFSVAPTGRTLIKGGTVLSM
                     DPAVGDFGSADVLIEGERIVEVRPGLEADGAEVIDASSMIVMPGFVDSHRHIWEGLLR
                     NIGTDVPLEGRSSYITFVLHKLAPAFRPEDAYVGNLVSALGAIDAGITTLLDWSHIQG
                     SPAHTDAVIQALRDSGLRAVFAYGFPWWGKWEERQPSWFVRAAKEHFTTKDQLLTLAL
                     AAPGPEFTDFEVSRDHWKLAREADARITTHVGVGSYGQDGKVQEMGEAGLLGPDTTYI
                     HCTTLNDTEIQMIVDSGGTVSLASPVEMMMGHGMPPIQKFLDRGLPPSLSVDVETNVP
                     SDMFNQMRSVLGLQRAMAIAEGKTPLTPMEVLACATIEGARANGLEAKVGTLTPGKQA
                     DVVLLRTDRINVTPLNDPATAVVAGMDTGNVDTVLIAGRVMKRGGELLHVDWPAVRRM
                     AAEARDYVVAKSGFKVPGI"
     gene            complement(5384..5992)
                     /locus_tag="VF097_10105"
     CDS             complement(5384..5992)
                     /locus_tag="VF097_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003994425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6263187.1"
                     /translation="MEIPSATMADIGDRLKVERVRQGISQRALADRLGLSASLISQIE
                     TGRSKPSVSTLYSMVTELGISLDSLFGDDEAAPAADQAAGPGVERGQEVVRPHERHVI
                     ELESGVRWERLTSDHEDEVDFLYVTYDVGGASSADGTLMRHSGREYGHLISGQLGVQI
                     RFKKYLLEPGDSIAFDSTQPHRLWNPGAVPARALWYVVGRDG"
     gene            6115..>6770
                     /locus_tag="VF097_10110"
     CDS             6115..>6770
                     /locus_tag="VF097_10110"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HEX6263188.1"
                     /translation="MATTEFPVATTSSPGVMGVDWEVRVDFERLRQYRLERTRQALDR
                     SELGALLLFETSNIRYVTSTQIGYWAFNKGERYALITRGGRPRIFDFGSAAKAHRLQL
                     PHMYDETNSVGGNTGLQGAIHPRVGLQARAAEEIKSIMKEDGVGDMPLGVDLAETSVF
                     LELQGAGIEVRDGQQVMADAREIKSEDELTLLTQACAMVDGVYQDIFEALRPGVRESD
                     "
BASE COUNT         1048 a         2262 c         2464 g          996 t
ORIGIN      
        1 gacgagatca acgacccagg agttggactg gatgcatcca gaggggggag gagtatgagg
       61 cggatcaagt tcttggggat cctcgtggtc cttgtgcttg tcgcggcggc gtgcggcgac
      121 gacggagacg acggcgccac aaccggcggc ggcgacaacg gcgggacgac caccaccggc
      181 gaggggccgc ttgccgagta cggcgagagc accgacgccg acccggcgct ggtggagaag
      241 gccctcgggc cggtggagga cgtaccggac atcgtgctgg cttccatcgc ccgggccgac
      301 cagaatccgg acgacgcgac gatcgagaag gccctggagt gctggaacaa cgtcgagtgc
      361 gaaaccggga gtggaggcga gctgatcatg ggcgtcgccg acggcgggcg cggcaacgtg
      421 tggcgccagg tgacgcacat ggaggcgatc ctccaggcgc tcacgtaccc ggagatcggg
      481 acgatcgtgt cccgggacgc ccagtgggcc gaggatccgg cggtggccga ggccgacatc
      541 aggttcctga tccagcgggg cgccgacttc atcatcggct acccggacca gggcacgaac
      601 atcgcggggg cgatcctcga ggccgaggcc gcgggcatcc cgtacatccc gtactcggcg
      661 ggatgggtgg ggctccccgg ccaggagggc gcgctcgtcc ccggtgagga ctacctcacg
      721 atcgtgggcg aggacctgtg cgagctcggt gagagcttcg cggacgtgct caacgaggag
      781 gtcggctcgg gcacgatcgg cgtcatgggc ggcacgcccg gcaacgcgct gtccgccggg
      841 tggcagcagt gcctcaggcc ggccctgaac gactccatcg agctggtggg gccgcccgcg
      901 gacacgtact gggtgaacga cttcgcgctc cagacggtcc tcggatggct gtcgacggac
      961 cccgatatca aggggtacgc ctacgagtac gccgacggca tgtacacggc gctggacgcg
     1021 tacgagcagc tcgacgtccc cgtcgaggac atcacgatcg cggtgcggac tgacgagcag
     1081 accctgttct gcgactgggc cgagcggaac gagccgagct acaagatcta cttctcggcc
     1141 ggcggcaact tccagtcgcg gatcgcggtc accgcggcga tgatgcacct caagggcgcg
     1201 gagatcccgg ccgagatcgt ggtgccacac gtgatgcgtg aggtcacggc ggaggactgc
     1261 gatccgaaca gggtcgagga gacggtgtcg aacacgtcgc tcgtgcccga cgacgtgctg
     1321 gcccagatgt acgaaggatg agcggaagcg gttccgaggg agggtcgacg cccggggacg
     1381 tggtcctcga gctgcgcggc gtctaccggc agtttggggc ggtccgagcg ctgaccgacg
     1441 tcagcttcga ctgccgcgcc ggcgagatcc acgccgtggt gggcgagaac ggctccggca
     1501 agtcgaccct cctcgggatc gccagcgggt tcgttgaccc cgaccgaggc acgatccgca
     1561 tcgggaggga gcgcctccgc cgggattccc cggcggaggc gcggaagctc ggactggcca
     1621 tggcctacca ggacacgtcg ctgatcctgg accagccggt gaagaacaac ctgtacctgg
     1681 cggcgccccc cgacaagcga cctccgttct accggaggaa gcgttgggct cgccgtctcc
     1741 tggccgagct cgacctggac ctcgagctct tccccgacat gccggcgggc gccctctcgc
     1801 tgtcagagcg acagctcttc gaggtggcca aggccctggt gaccgacccg ctcgtcctgc
     1861 tgctcgacga gcccacgacc gcgctgggcc cccacgaggt cgaggccctc caccggacgc
     1921 tcgccgcgtg cagggagcga ggagtggggg tcgtgtacgt cagccaccga ctgccggaag
     1981 tcctcgaggt cgcggatcgc gtcaccgtcc tgcgcgatgg gcgcagccag gggaccttcg
     2041 acgcgggagc ggtggcggag ccggagctcg tggacctgat cgtggggcgg ccgttcgagg
     2101 ccgccttccc gcaggcgtcc ccgggcgctg gggagccgcg cgacgtcctc gtcgtggatg
     2161 gcctccaggg gcagtccttc gggccggtga gcttcacgct ggagagcggc gagatcgtgg
     2221 ggatcgccgg ggccgagggc aacggccagc cccagctctt cgagtgcctg tcggggcggc
     2281 aggctccgaa ggcgggccgc gtcgtctgcg acggcaagga gctgatgctg atctcgaccc
     2341 acgaggcggt gagcgcgggg ctcatggtgc tccccggaga tcgcaagcac gaggcgctga
     2401 tgccggtgct cggggtcagg gtgaactcga cgatccagtc gctccggcgg ttcggggtcc
     2461 tcgggatcct gcggaggagg aaggagcgga ggtcggtgga agacctgatc gggcggctcg
     2521 agatccgcac gccatcgctc gagcagcccg tgcagttcct ctccggcggc aaccagcaga
     2581 aggtcgccgt ctcgagatcc ttcctcaagg agccctcggt gatcctggcc tacgagccga
     2641 ctcagggcgt cgacgtgggc tcacggttcg acatctacga ggccctccgg tcgcggagcg
     2701 acgccgggac ggccctcctc gtgaagtcga gcgacccgct cgagctgacg gggctctgcg
     2761 accgggtgct ggtgatgtcc cgcgggcaga tcgtggagga gatccccggc gacgagctca
     2821 ccgagcaccg catcgtggag gcgatcgtcc gggggccggg gatgtcgcgc gcggcgcggt
     2881 cgccgctcgg cgtggccatg ccgaaggcgt cccggagcgg ctcgtgagcg atcggatcga
     2941 cgccgccagg cgggcggtgc gcaaggtcct gcgggacgcc cgcgacgtca acaagtggcg
     3001 gaagatcgtc cggttccgcc tctggatgcc ggtggcgctg caggtgctgc tgctgatcgc
     3061 ggtggcctgg tacacgacca cgaggttccc ggggttcatc aacaccacga acgtcacgaa
     3121 catcctcatc ttggcggcgc ccctggcggt ggtggcgatc ggccagaccc aggccctgct
     3181 cgtcggctac ctcgacctct cggtcggggc gatggtgacc ttcggcgtcg tgatcgcgtc
     3241 gttcctgatc ggccccgacg ccggcgccgc gcagatcgcg ctcggcgcgg gcgtcatctt
     3301 cctggcgggg ctgggcctcg ggctcgtcaa catggtgctc gtcctcgggt tcaagatccc
     3361 gtcgatcatc gcgacgctgg ccaccctcag catcctcaat gggatctcgc tcacgctgcg
     3421 ggacacgccc ggggggatca tcaaccggga cttcacggcc tcgctgggga cgaccctcgg
     3481 ccccttcccg atcagcttca tcctgatcgt gctggcggcc ggcgcgctcg actactggct
     3541 gcacgccagc ggctcgggcc tccagctgcg ctcgaccggg ttcgacgagc ggtcggcgaa
     3601 gcggagcggc gtccgcacga agtgggtgca gggcagggcc ctggtgctgt cggcgctctt
     3661 cgccgccctg gcctcgttcc tcgtcatgac gcggtcgggg gtggggaacg cggcgatcgg
     3721 cgacagctac gtcctcaaca gcatcaccgc cgccgtgctc ggcggagcgg cgctgtccgg
     3781 cgggcgggcc acgttcgtgg gcacgctcgt ggcctcggtg ctgctggcgc tcatcatcag
     3841 cgccctcccc ttcctgggcc tcggggcgga gcacggcctg atgatcatcg gcgtcctcgt
     3901 cctgctggcc atcgtgctgt tccaggccgg cgacatcaag gagctcgtga agcggaacta
     3961 caagcggggc cgacgcctgg tgatcggcag ccgcagcccg aagcgggccg agatccccga
     4021 cttctacccg tcgggcccgg acttcagcgt cgcgcccacg gggaggacgc tgatcaaggg
     4081 cgggaccgtg ctgagcatgg accccgccgt gggcgacttc ggttcggccg acgtcctcat
     4141 cgagggcgag cggatcgtgg aggtccgccc cggcctggag gccgacggcg cggaggtgat
     4201 cgacgcctcg agcatgatcg tgatgcccgg cttcgtcgac tcgcaccggc acatctggga
     4261 ggggctcctc cggaacatcg gcaccgacgt cccgctggag ggccggtcca gctacatcac
     4321 gttcgtgctg cacaagctgg cccctgcctt ccgccccgag gatgcctacg tcggcaacct
     4381 cgtctccgcc ctgggggcga tcgacgccgg catcacgacg ctcctcgact ggtcccacat
     4441 ccagggctcg cccgcccaca cggacgcggt gatccaggcg ctgcgggact cggggctgcg
     4501 ggccgtcttc gcctacgggt tcccgtggtg ggggaagtgg gaggagcggc agccgagctg
     4561 gttcgtccgg gcggcgaagg agcacttcac cacgaaggac cagctgctca cgctggccct
     4621 cgccgcgccg ggccccgagt tcaccgactt cgaggtctcc cgcgaccact ggaagctggc
     4681 ccgcgaggcc gacgcccgca tcacgaccca cgtcggggtg gggagctacg gccaggacgg
     4741 caaggtccag gagatgggcg aggcggggct gctcgggccg gacaccacct acatccactg
     4801 caccaccctg aacgacaccg agatccagat gatcgtggac tcgggaggca cggtctcgct
     4861 ggcctccccg gtggagatga tgatgggtca cggcatgccg cccatccaga agttcctcga
     4921 tcgagggctt ccccccagcc tcagcgtcga cgtcgagacg aacgtcccga gcgacatgtt
     4981 caatcagatg cggtcggtgc tcgggctcca gcgcgcgatg gcgatcgccg aggggaagac
     5041 cccgctcacc ccgatggagg tcctggcgtg cgccacgatc gagggagcca gggcgaacgg
     5101 gctcgaggcg aaggtgggga cgctcacgcc ggggaagcag gccgatgtgg tcctgctccg
     5161 aaccgaccgg atcaacgtga cgccgctgaa cgacccggcc acggccgtcg tggcgggcat
     5221 ggacacgggc aacgtggaca ccgtcctcat cgccggccgg gtgatgaaac gcgggggcga
     5281 gctcctgcac gtcgactggc cggccgtgcg gcggatggcc gccgaggcca gggactacgt
     5341 cgtggcgaag tcgggcttca aggtccccgg catctgaggc gggtcagccg tcccggccca
     5401 ccacgtacca gagcgcccgc gcagggacgg cccccgggtt ccacaggcgg tggggctgcg
     5461 tggagtcgaa ggcgatcgag tcgcccggct ccagcaggta cttcttgaag cggatctgca
     5521 ccccgagctg gccggagatg aggtggccgt actcgcggcc cgagtgccgc atcagggtcc
     5581 cgtctgccga ggatgccccc ccgacgtcgt aggtcacgta caggaagtcg acctcgtcct
     5641 cgtgatcgct ggtcagccgc tcccaccgca cccccgactc cagctcgatg acgtgtcgct
     5701 catggggcct gaccacctcc tggccgcgct cgacacccgg gcccgcggcc tggtctgcgg
     5761 ccggcgccgc ctcgtcgtcg ccgaagaggc tgtcgagcga gatgcccagc tcggtgacca
     5821 tggagtagag cgtgctgacc gagggcttcg atcggccggt ctcgatctgg gagatcaaac
     5881 tggcggagag ccccagccga tcggccagcg cccgctggct gatcccctgg cgaacccgct
     5941 ccaccttcaa tcgatcgccg atgtcggcca tcgtggccga ggggatctcc atgtccgggt
     6001 cagtgtaccg tgagccgaca tccccttgcg cggcctgcgg cgacgcacta tgttcgggta
     6061 gtcacactgc acggggcgtt cagcgacgct ggagggccga ggagggggag gagcatggcg
     6121 acgaccgagt tcccggtggc gacgacgtcc tcgccgggcg tcatgggggt cgactgggag
     6181 gtgcgggtcg acttcgagcg cctccggcag taccggctgg agcgaacccg ccaggccctc
     6241 gaccgctccg agctcggggc gctgctcctc ttcgagacga gcaacatccg ttacgtcacc
     6301 tcgacccaga tcgggtactg ggcgttcaac aagggcgagc gttacgcgct catcacgcgc
     6361 ggcggcaggc cccgcatctt cgacttcggg tcggcggcga aggcgcaccg cctccagctc
     6421 ccgcacatgt acgacgagac gaactcggtc ggtgggaaca ccggcctgca gggcgcgatc
     6481 caccctcgcg tcgggctcca ggccagggcg gcggaggaga tcaagtcgat catgaaggag
     6541 gacggggtcg gcgacatgcc gctgggcgtg gatctggcgg agacgtcggt cttcctcgag
     6601 ctccagggcg ctggcatcga ggttcgggac ggccagcagg tgatggccga cgccagggag
     6661 atcaagagcg aggacgagct gaccctcctc acccaggcct gcgccatggt ggacggcgtc
     6721 taccaggaca tcttcgaggc gctgaggccg ggcgtgcgcg agagcgacat
//
LOCUS       DASUTZ010000236         5834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_472362, whole genome shotgun sequence.
ACCESSION   DASUTZ010000236 DASUTZ010000000
VERSION     DASUTZ010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5834
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472362"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="VF097_10115"
     CDS             complement(<1..946)
                     /locus_tag="VF097_10115"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX6263189.1"
                     /translation="MAEHTKYIFVTGGVSSSLGKGITAASLGRLFRSRGLRVALQKLD
                     PYVNVDPGTMNPFEHGEVFVLEDGAETDLDLGHYERFVDENLYRGSNVTTGAIYSSVI
                     AKERRGEFLGKTVQVIPHVTDEIKARIRGLARSTDADVIIVEVGGTVGDIESLPFLEA
                     IRQLRNEVGRDNCAFVHVSLLPHIGPTGELKTKPTQHSVRELRSIGIQPDAIVCRSDR
                     PIGHGLKEKISLLCDVEIAGVVSAVDADSLYEVPLTLHEEGLDTFLARHLRIDAEPDL
                     TEWRALVDRIRSAEGEVTIAVVGKYVNLPDAYLSVIEAL"
     gene            complement(1024..1833)
                     /locus_tag="VF097_10120"
     CDS             complement(1024..1833)
                     /locus_tag="VF097_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263190.1"
                     /translation="MTWLSLTAGALVGAALAWFSQLRPPRGLVRENHAGLPVPAVLGL
                     ALVGGAVVGHALGRPEAEAGALEVAAVLALLVVFVVGVLDDLAGGGPRGLGGHLRSLA
                     SGRPTTGILKLVAGIAAGVAAAVAAGGGPLRVVAGALVVATAVNVFNALDVVPGRSLK
                     WGVIALAVCVPWAWGTRVGSLVAAALGAAVASLPFDLGERAMLGDGGSNPLGFLVGVA
                     LLVLLPTSGLLVAAAALLALQVAAETVTISRLIEATPPLRWLDGLGRWRRS"
     gene            complement(1830..2501)
                     /locus_tag="VF097_10125"
     CDS             complement(1830..2501)
                     /locus_tag="VF097_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6263191.1"
                     /translation="MLALAVVAARDEEGRVGETVRAIRELPVDEIVVVDDGSRDGTAA
                     EAVAAGARVLVAPRPMGKGEALEAALDRAPAADAYLLLDADLGATASAAVPLLNLVRD
                     GKADLAIAVLPRQPGHGGFRIVKRISGRLIRSLSGFRAREPMSGQRAMTREVLEAVRP
                     LAPGFGVETAMTIDAARAGFRVVEVEVPMAHRVTRRDLAGFAHRARQGRDVLAAAWPR
                     AIGRR"
     gene            complement(2501..3406)
                     /locus_tag="VF097_10130"
     CDS             complement(2501..3406)
                     /locus_tag="VF097_10130"
                     /inference="COORDINATES: protein motif:HMM:NF022824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HEX6263192.1"
                     /translation="MISFRFHLVSLVAVFLALGLGVLTGTTVLNRGIVAQLERTTDRL
                     SEQSGQLREEVAQLEARLGEWGDFGAEIQAYVVPGRLAEEEVVLVTQEGTDPAAVDAA
                     RQALELAGARVRLDLTVRLRMALTDTGDRETIARVLEADTEDDPGALRDRAAEMIADQ
                     VAFGSTSGNVIDRLADEGFVTVERTADDEPGPREPEPMIVVVGGGTDETPLPPEAFLV
                     PLVERLVLDGQSVAAAESATVLVPFVPLLREGPVSDRLVTQDNVDQVPGEVSLVLALE
                     DLVELGEAGHYGVKAGANGLVPSLG"
     gene            complement(3449..4597)
                     /gene="steA"
                     /locus_tag="VF097_10135"
     CDS             complement(3449..4597)
                     /gene="steA"
                     /locus_tag="VF097_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HEX6263193.1"
                     /translation="MLGLGTRRRAGAARVDRRTKRLLTRVQRGEIAVIDHEDLDRVAA
                     EGLVAREVAAVVNASPSITGRYPNLGPLLLVEAGIPLVDGVGPLLMEKLREGMQVRVE
                     DDRVYADDALVGVGVRQTPETVRRAMEEAREGLSEQLQAFARNTLDYMQRERDLLFGG
                     EGMPGLDEDLAERHVLVVVRGSNYREDLAALRSYIREVKPVLMGVDGGADALLEAGYR
                     PDIVIGDMDSVSEEALRSGAQIVLHAYPDGAAPGRARLEALGLPFSVLRSSGMSEDVA
                     YLLAHEKGAELIVSVGSHGNLREFLDKGRKGMSSTFLVRLRVGEILVDAKGVSRMYRS
                     RIRGRDVAMLVGAALFSMVAVVAVSQPLRLFFKQLWELIRQALFSIGF"
     gene            complement(4548..5264)
                     /locus_tag="VF097_10140"
     CDS             complement(4548..5264)
                     /locus_tag="VF097_10140"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX6263194.1"
                     /translation="MRARWVAVAALLALGVWIPALVGVAGGARSTPPRRVTEPSVEDG
                     SAPPATPVFAEALGIRLHLPASSPRLVAFHEASMEGALPMLPHGRCRPCRHPRFRPGP
                     AADLRYAVMDPRGRGTPPTSAVDVVVGGESRVLSPVSGRVVEVRRYRLYGRHPDVRVA
                     IAPDGAPGIEVVLLHLRSGRLAPGDRVVAGVTPLGRARSLPFRSQVDRYAAGRRPHVH
                     VEVVDARARDQAQGRRGPGG"
     gene            complement(5261..>5834)
                     /locus_tag="VF097_10145"
     CDS             complement(5261..>5834)
                     /locus_tag="VF097_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016468070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX6263195.1"
                     /translation="LRSRRTAAAPELAERLRAELEELGMSGARVEVDLRSQEPGPDGT
                     EAVELLFAGGPGQTPQPLRKVASGGELSRTMLACRTILADLDEVPTLVFDEVDAGIGG
                     RAGVAVGRRLARLAGSRQVLVVTHLPQIACFADRHFHVSKHDGRTSVRALEGEERVAE
                     LSRMLSGMPTEEAATHAEQLLAEAAKEKRR"
BASE COUNT          853 a         2258 c         2018 g          705 t
ORIGIN      
        1 tcagggcctc gatcaccgag aggtaggcgt cgggcaggtt cacgtacttc ccgacgacgg
       61 cgatcgtcac ctcgccctcc gccgagcgga tccggtccac gagcgcccgc cactcggtga
      121 ggtcgggctc ggcgtcgatg cgcaagtggc gggcgaggaa cgtgtcgagc ccctcctcgt
      181 gcagcgtcag cgggacctcg tacagggagt ctgcgtccac cgccgagacg acccccgcga
      241 tctcgacgtc gcagaggagc gagatcttct ccttgaggcc gtggccgatc ggacggtccg
      301 agcggcagac gatcgcatcg ggctggatac cgatcgaccg cagctcgcgc acggagtgct
      361 gcgtgggctt cgtcttcagc tcccccgtgg gaccgatgtg gggcagcagg gagacgtgca
      421 cgaacgcgca gttgtcccgg cccacctcgt tgcggagctg gcggatcgcc tccaggaacg
      481 ggagggactc gatgtcgccc accgtccccc cgacctcgac gatgatcacg tcggcgtcgg
      541 tggatcgggc cagcccacgg atccgggcct tgatctcgtc ggtgacgtgc gggatgacct
      601 ggacggtctt gcccaggaac tcgccgcgcc gctccttggc gatcaccgag gagtagatgg
      661 ccccggtggt gacgttggag ccgcggtaga ggttctcgtc cacgaagcgc tcgtagtggc
      721 caagatcgag gtcggtctcc gcgccgtcct cgaggacgaa gacctccccg tgctcgaagg
      781 ggttcatcgt cccggggtcg acattgacgt agggatcgag cttctggagc gcgacacgga
      841 gcccgcggga gcggaagaga cggcccaggg acgcggcggt gatgcccttc ccgaggctcg
      901 acgacacccc gccggtaacg aagatgtact tcgtgtgctc agccacctcg ggaggccatg
      961 ctatcagacg gtccaggaag ggtgtggctc ttcggtggcc ttctcgacgg atctggccca
     1021 ggtctaggag cggcgccacc gccccagccc gtcgagccac cgcaggggcg gcgtggcctc
     1081 gatcaggcgg ctgatcgtga cggtctcggc ggcgacctgg agcgccagca gcgcggccgc
     1141 ggcgacgagc agccccgagg tcggcagcag cacgagcagg gccaccccca cgaggaagcc
     1201 gagggggttg gagccgccgt cgccgagcat cgcccgctcc ccgaggtcga acgggagcga
     1261 ggcgacggcg gctcccaagg cggccgccac gagcgagccg accctcgtcc cccaggccca
     1321 gggcacgcac acagccagcg cgatcacccc ccacttcagc gaccggcccg ggacgacgtc
     1381 cagggcgttg aagacgttga cggcggtcgc caccaccagg gccccggcca cgacccggag
     1441 cggccccccg ccggcggcga ccgccgcggc cacccccgcc gcgatcccgg ccacgagctt
     1501 caggatcccc gtcgtgggcc ggccgctggc caggctccgc aggtggcccc ccaggccccg
     1561 aggccccccg ccggccaggt cgtccagcac cccgaccacg aagacgacca gcagcgcgag
     1621 gaccgcggcc acctccaagg cgccggcctc ggcctccggc cgacccaggg catgtcccac
     1681 gaccgcgcca cccacgaggg cgaggcccag caccgcgggc accggcagcc ccgcgtggtt
     1741 ctcccggacg agcccccgcg ggggcctgag ctgcgagaac caggccagcg ccgcccccac
     1801 cagggctccc gccgtcagcg agagccaggt catcgccgcc cgatcgcgcg aggccaggcc
     1861 gccgccagga cgtccctccc ctgccgggct cggtgcgcga acccggcgag gtcgcgccgg
     1921 gtgactcgat gggccatcgg gacctcgacc tccaccaccc ggaatcccgc ccgcgccgcg
     1981 tcgatcgtca tggcggtctc cacgccgaag cccggcgcca gcgggcgcac cgcctccagc
     2041 acctcccggg tcatggcccg ctggccggac atcggctcgc gggcccggaa cccggacaga
     2101 ctccggatca gccggcccga gatccgcttc acgatccgga agcctccgtg gcccggctga
     2161 cgagggagca cggcgatcgc caggtcggcc ttcccatccc gaacgaggtt cagaagcggg
     2221 acggcggccg aggccgtcgc ccccaggtcg gcgtcgagca gaaggtaggc gtcggctgcg
     2281 ggcgcccggt cgagcgcggc ctccagcgcc tcccccttcc ccatggggcg cggggcgacc
     2341 agcacccggg cgcccgcggc gacggcctcg gccgccgtcc cgtcccggga cccgtcgtcc
     2401 accaccacga tctcgtccac ggggagctcg cggatcgccc ggacggtctc gcccacccgg
     2461 ccctcctcgt cgcgggccgc cacgaccgcc agcgcgagca tcagccgagc gagggcacca
     2521 ggccgttcgc gcccgccttc accccgtagt ggccggcctc ccccagctcg accaggtcct
     2581 cgagggccag cacgagcgag acctcgcccg ggacctgatc cacgttgtcc tgcgtgacga
     2641 gccgatccga cacgggaccc tcgcggagca ggggaacgaa cggcaccagc accgtcgcgc
     2701 tctcggcggc cgccacgctc tggccgtcga ggacgagtcg ctcgaccagc ggcacgagga
     2761 acgcctccgg aggaagcggg gtctcgtccg tgcctccgcc cacgaccacg atcatcggct
     2821 ccggctccct ggggccgggc tcatcgtccg ccgtgcgctc caccgtcacg aacccctcgt
     2881 cggccaggcg gtcgatcacg ttcccgctgg tggatccgaa agccacctga tcggcgatca
     2941 tctcggccgc gcggtcccgg agcgcgcccg ggtcgtcctc cgtgtcggcc tcgagaaccc
     3001 gagcgatggt ctcccggtca ccggtgtccg tgagggccat ccgcaggcgg accgtgaggt
     3061 cgaggcggac gcgggcgccg gcgagctcga gggcctgccg ggcggcgtcc accgccgcgg
     3121 ggtcggtgcc ctcctgggtc accagcacga cctcttcctc cgccagcctg cccggcacca
     3181 cgtacgcctg gatctccgcg ccgaagtcgc cccactcgcc cagcctcgcc tcgagctggg
     3241 cgacctcctc gcgaagctgg cccgactgct cggagagtcg atccgtggtc cgctcgagct
     3301 gcgccacgat gccccggttg aggacggtgg tgcccgtcag cacgccgagc cccagggcga
     3361 ggaacaccgc caccagcgac acgaggtgga agcggaacga gatcatcggc gcccggcccg
     3421 gatcatgcgc cgcctatcgt agccccgatc agaagccgat cgagaagagc gcctgccgga
     3481 tcagctccca cagctgcttg aagaagagac gcaggggctg ggacacggcc accacggcga
     3541 ccatcgagaa cagcgcggct cccacgagca tggcgacgtc ccgtccccgg atgcggctcc
     3601 ggtacatccg ggagaccccc ttggcgtcca cgaggatctc ccccacgcgg agccgcacca
     3661 ggaacgtgct cgacatgccc ttccgcccct tgtccaggaa ctcccggagg ttcccgtggc
     3721 tccccaccga gacgatgagc tccgccccct tctcgtgggc caggaggtag gccacgtcct
     3781 cgctcatccc cgaggatcgc agcaccgaga acggaaggcc gagcgcttcg aggcgggccc
     3841 ggccgggggc ggcgccgtcg ggataggcgt gcaggacgat ctgggcgccc gaccggagcg
     3901 cctcctcgga gacgctgtcc atgtccccga tcacgatgtc ggggcggtag ccggcctcga
     3961 gcagggcgtc ggcgccaccg tccaccccca tcaggacggg cttcacctcg cggatgtagg
     4021 aacggagcgc tgccaggtcc tcccggtagt tcgagccccg cacgaccacg agcacgtgcc
     4081 gctccgccag gtcctcgtcg aggccgggca tcccctcccc tccgaagagg aggtcgcgct
     4141 cccgctgcat gtaatccagc gtgttccggg cgaaggcctg gagttgctcg gagaggccct
     4201 cccgcgcctc ctccatcgcc cggcgaacgg tctccggggt ctggcggacg cccaccccca
     4261 ccagggcgtc gtcggcgtag acgcggtcgt cctccacccg cacctgcatc ccctcgcgga
     4321 gcttctccat gaggagcgga cccaccccgt ccacgagggg gatgccggcc tcgaccagga
     4381 ggagcggccc caggttcggg tagcggccgg tgatcgacgg gctggcgttc accacggcgg
     4441 cgacctccct ggccaccagg ccctccgcgg cgacgcggtc gaggtcctcg tggtcgatca
     4501 cggcgatctc cccgcgctgc acccgggtga ggaggcgctt cgtgcgccta tccacccggg
     4561 ccgcgccggc cctgcgcctg gtcccgagcc cgagcatcca ccacctccac gtgcacgtgc
     4621 gggcgccggc ccgcagcgta ccgatcgacc tgggagcgga acgggagcga cctggcccgc
     4681 cccagcggcg tgacgcccgc cacgacgcga tcccccggtg caagcctacc gctgcggagg
     4741 tgcaacagga cgacctcgat ccccggcgcg ccgtcggggg cgatcgccac ccggacgtcg
     4801 gggtgacgcc cgtagaggcg gtaccgacgg acctcgacca cgcggccgct cacgggggag
     4861 aggacccggg actcgccgcc cacgacgacg tccaccgccg acgtcggcgg cgtccccctc
     4921 cccctggggt ccatcaccgc gtatcgcagg tcggccgcgg gcccgggccg gaagcgtgga
     4981 tggcgacagg ggcggcaccg tccgtgcgga agcatcggga gcgcgccctc catcgaagct
     5041 tcgtggaagg ccacgagccg aggcgacgag gccggcaggt ggagccggat ccccagggcc
     5101 tcggcgaaca cgggggtggc gggcggcgcg gatccgtcct cgaccgacgg ctccgtcacc
     5161 cggcggggcg gggtggatcg cgcgccgccc gccaccccga cgagagccgg gatccacaca
     5221 ccgagggcga gcagggccgc gacggcgacc cagcgggccc tcaccgccgc ttctccttgg
     5281 ccgcctcggc caggagctgc tcggcgtgcg tggccgcctc ctcggtcggc atccccgaga
     5341 gcattctcga cagctcggcc acccgctcct ccccctccag agcgcggacc gacgtcctcc
     5401 cgtcgtgctt cgagacgtgg aagtggcgat cggcgaagca cgcgatctgc ggcaggtggg
     5461 tgacgacgag gacctggcga gacccggcca gcctggccag tcgtcgcccc acggccactc
     5521 ccgcccggcc gccgatcccc gcgtcgacct cgtcgaagac cagggtgggg acctcgtcga
     5581 ggtcggcgag gatcgttcgg cacgcaagca tcgtccggga gagctcgcct cccgaggcca
     5641 ccttgcgcaa gggctgaggg gtctgcccgg ggcccccggc gaacagcagc tcgacggcct
     5701 ccgtcccgtc ggggccgggc tcctgcgagc ggaggtccac ctcgacccgg gcccccgaca
     5761 tccccaactc ctcgagctcg gcccggagcc gctcggccag ctcgggggcg gccgcggtcc
     5821 tccgcgagcg gagg
//
LOCUS       DASUTZ010000237         2451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_345201, whole genome shotgun sequence.
ACCESSION   DASUTZ010000237 DASUTZ010000000
VERSION     DASUTZ010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345201"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..>2451
                     /locus_tag="VF097_10150"
     CDS             5..>2451
                     /locus_tag="VF097_10150"
                     /EC_number="2.5.1.147"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional FO biosynthesis protein CofGH"
                     /protein_id="HEX6263196.1"
                     /translation="MAPTPAEVRRALARAESGKSLSPGDVAALLAARGEDLDRLLAVA
                     SRLRELGWGRTITYSPKVFIPLTMLCRDHCHYCTFAKPPARIDAPYLTPEQAVEIAEA
                     GRLAGCTEALFTLGDRPEDRYPEARAWLEERGHAGTLDYVREVAIRVIEETGLLPHLN
                     PGIMSWEEMARLKHVSASMGLMLEQATGRLLEKGMAHHGSPDKVPAVRLRMIEEAGRL
                     SIPFTTGMLIGIGETLRERAETLLAIREAERRYRHVQEVIVQNFRAKPRTRMRDHPEP
                     DRDELLAAIATARVVLGPRVSVQAPPNLSDEGFPRLLDAGLNDWGGVSPVTPDHVNPE
                     KPWPKLERLRAATEARGFVLRPRLPVYPRYVLKPDPWISGKMRAPLQALAGPDGYPSG
                     RRPEPIPWQDPDVQWKPRQIDLAFAKAAGAGLREDALEVYGDFEQLDVTRRWAQERMA
                     PERLDAEIRAALAKASGGLPVTDGEALALFRAEGAALDALTSVADDLRHETVGEEVTY
                     VVNRNINFTNVCYTGCRFCAFAQREIDPESYTLTLREVADRAEEAAAAGATEVCMQGG
                     LHPDLPGTFYFDLLDAVKARVPEIHVHAFSPMEVLNGATRLGISFGEFLEECLRRGLG
                     SIPGTAAEILDDDVRWLLTKGKLPADAWEEIVRTAHEVGLPSSSTIMYGHVDAPPHWV
                     AHLRRLGSIQDDTGGFTEFVPLPFVHQNSPIYLAGKARPGPTHEENRRMHAIARILLH
                     GRIPNVQVSWVKLGLEASQLMLRGGANDLGGTLMEETISRMAGAEWGIRKEPEELEQA
                     IRAIGRVPAQRTTTYGRA"
BASE COUNT          353 a          922 c          855 g          321 t
ORIGIN      
        1 ggccatggct cccacccccg cggaggttcg gcgggccctg gcccgcgccg agtcgggcaa
       61 gtccctgtcg cccggagacg tggccgcgct gctcgcggcc cggggggagg acctcgaccg
      121 gctgctggcc gtcgcctccc ggctccgcga gctcggctgg ggccgcacga tcacctactc
      181 cccgaaggtc ttcatcccgc tcacgatgct gtgccgtgac cactgtcact actgcacgtt
      241 cgccaaaccc ccggcgcgga tcgatgcccc gtacctgacg cccgagcagg ccgtcgagat
      301 cgcggaggcg gggcgcctgg cgggctgcac cgaggcgctg ttcacgctcg gggaccgccc
      361 cgaggaccgc taccccgagg cccgggcctg gctcgaggag cgaggccacg ccggcaccct
      421 cgactacgtc cgggaggtgg cgatccgcgt gatcgaggag accggcctgc ttccgcacct
      481 caaccccggg atcatgtcct gggaggagat ggcccgcctc aagcacgtct cggcctcgat
      541 ggggctgatg ctggagcagg ccacgggccg cctcctggag aaggggatgg cccaccacgg
      601 gtcccccgac aaggttcccg ccgttcgcct gcgcatgatc gaggaggcgg gccggctctc
      661 gatccccttc accacgggga tgctgatcgg gatcggggag actctccgag agcgggccga
      721 gacgctgctt gcgatccgcg aggccgagcg ccggtaccgc cacgtccagg aggtcatcgt
      781 ccagaacttc cgggccaagc cccgcacgcg gatgcgggac caccccgagc cggaccggga
      841 cgagctgctg gccgcgatcg cgaccgcccg ggtggtgctc ggcccccgcg tgagcgtcca
      901 ggccccgccc aacctctccg acgaagggtt cccccggctc ctcgacgccg ggctgaacga
      961 ctggggcggg gtctccccgg tcacccccga ccacgtcaac cccgagaagc cctggcccaa
     1021 gctggagcgc ctccgggccg cgaccgaggc ccgaggcttt gttctccgcc cgcgcctgcc
     1081 cgtctacccg cgctacgtgc tcaagcccga cccctggatc agcgggaaga tgcgggcccc
     1141 cctccaggcc ctggccgggc ccgacggcta cccgagcggg cggcgccccg agcccatccc
     1201 gtggcaggac cccgacgtgc agtggaagcc gcgccagatc gacctcgcgt tcgccaaggc
     1261 ggcgggggcc ggcctccggg aggacgccct cgaggtctac ggcgacttcg agcagctcga
     1321 tgtcacccgc cggtgggccc aggagcgcat ggcgcccgag cgactcgacg ccgagatccg
     1381 ggccgcgctg gccaaggcct cgggcggtct ccccgtcacc gacggcgagg ccctggcgct
     1441 gttccgggcc gagggcgcgg cgctcgacgc cctgacctcc gtggccgacg acctgcgcca
     1501 cgagaccgtg ggcgaggagg tcacctacgt ggtcaaccgc aacatcaact tcaccaacgt
     1561 ctgctacacg gggtgccgct tctgcgcctt cgcccagcgc gagatcgatc ccgagtccta
     1621 cacgctcacc cttcgcgagg tggccgatcg ggccgaggag gccgccgcag ccggggccac
     1681 cgaggtctgc atgcagggcg ggctccaccc cgacctgccc gggaccttct acttcgacct
     1741 gctggacgcg gtgaaggccc gcgtgcccga gatccacgtc cacgccttca gcccgatgga
     1801 ggtcctgaac ggggccacgc gcctggggat ctcgttcggg gagttcctcg aggagtgtct
     1861 gaggcgtggc ctggggtcga tccccggcac cgccgccgag atcctcgacg acgacgtgcg
     1921 gtggctgctg acgaagggaa agctgccggc cgatgcctgg gaggagatcg tccggaccgc
     1981 gcacgaggtc ggcctgccct ccagctccac gatcatgtac gggcacgtgg acgccccgcc
     2041 gcactgggtt gcgcacctgc gccgcctggg gagcatccag gacgacaccg ggggcttcac
     2101 cgagttcgtc ccgctgccgt tcgtccacca gaactcgccg atctacctgg ccgggaaggc
     2161 ccgccccggt cccacccacg aggagaaccg ccggatgcac gccatcgccc ggatcctgct
     2221 gcacgggcgc atccccaacg tccaggtctc gtgggtgaag cttggcctgg aggcgtcgca
     2281 gctgatgctg cggggcgggg ccaacgacct cggcggaact ctgatggagg agacgatctc
     2341 gcggatggcc ggcgcggagt gggggatccg caaggagccc gaggagctcg agcaggcgat
     2401 ccgggccatc ggccgggtgc ccgcccagcg caccacgacg tacggtcgcg c
//
LOCUS       DASUTZ010000238        13759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1642076, whole genome shotgun sequence.
ACCESSION   DASUTZ010000238 DASUTZ010000000
VERSION     DASUTZ010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13759
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642076"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1202)
                     /locus_tag="VF097_10155"
     CDS             complement(18..1202)
                     /locus_tag="VF097_10155"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX6263197.1"
                     /translation="MSDFRTLGASTPVIRALEAHGIRAPFPIQTLVLPDAMAGHDVLA
                     KSRTGSGKTLAFAIPIVETTDPKDPRPTALVLVPTRELASQVREDFEPIAKARGLRVA
                     VAYGGVGIREQANRAAKAHILVATPGRLEDLAQRKLVKLDGIRVLVLDEADRMLDMGF
                     QPQVDRIVRRVPKDRQTMFFSATLDGHVGFLASRYTTDAVRHEIETLIETVDEASHRF
                     LPVPGGDKLPVLVDLLKEERGLALIFVRTKRGADRLVTRLKSKGVRAEAMHGDMRQQL
                     RERTLAKFEAGSIDALVATDVAARGLDLDDITHVINYDPPEDGKGYVHRVGRTARAGR
                     AGTGVTLVTPDKQGDVGRIARHLKLGDEFEVEGMKVPAPRAVFTSSRRGRGRGRVRPR
                     RR"
     gene            complement(1348..2073)
                     /locus_tag="VF097_10160"
     CDS             complement(1348..2073)
                     /locus_tag="VF097_10160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX6263198.1"
                     /translation="MTTSVDEGTDPIDSLYSARLVARRRLLSRLVKARDLERQRIASE
                     IHDEPLQVLSTISTRLQGLRQAQEDPALRSEIGGIQETLTAAIEHLRGLTFELARGPR
                     EEFGLLAELRAMLERAAEDGDLEARLSERVIEEPPFETASAAYRILQEALANIQKHAR
                     ATRVDVAVESDDGLFLRIEDDGVGFEPEGVVPSPSHMGIAMMRQRAMAAGGWLRVDSE
                     AGKGTAIELHLPGRVPAEPVTIG"
     gene            complement(3767..5686)
                     /locus_tag="VF097_10165"
     CDS             complement(3767..5686)
                     /locus_tag="VF097_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263199.1"
                     /translation="MSRVPAARRAPTRGSRRRPRAAWPAALTWAGGVALLLLGVFLAH
                     PLLEGHRLPLGPDGPVYVWWSRFVEVEGLEAVRRPGVPALILALTSLLVTDPLRIVAG
                     LEPALAAAVGLAGGAVAEAVLGPDRVRFGTTSLLVGAFAGLLAAGWMANLVLAALFLT
                     ALAAFAVAGRSWRGVGFGAVALAAAGVAHSLFLAVALGIVTGAVILLAPDALRRIRAG
                     VRPLDTAAMRISVGAAAGGGGALLAVGLLAGGPPAPGDTSQDQVFRQIGMGGTLGERY
                     RERLWGDARRMAIPAAAAILGAAGTGLLDGVDREHRRYAISVAASWVAVTVVGAAVLW
                     ATGVGPPGRLLTFAYVLPLAAAAAAASAMRRGGWIRIAAAAAAAIVAASSLWGWYRQT
                     PFMSEEELRSVGSVEPVLDELPEDTPILVAVDTEEFAAAFHVARFGNVIRAALPPERI
                     AETSLVVGDAEDVLAGRPGTRSEDPEYVGIAEAYLERARPVLDEAAIIVLEPFHPGFA
                     DARRLGEPLSPHAVLLRAPEGFTAPAADRSGSDPGISPWAWVGLSLLAALGLALLGLG
                     WSAWGLHGAGPLAVLGAAPAAGLGVLILGGLAADRLTLPAGSGGAALAGTLALVGYFL
                     AARHRPVEDPEHPRA"
     gene            5753..7138
                     /locus_tag="VF097_10170"
     CDS             5753..7138
                     /locus_tag="VF097_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX6263200.1"
                     /translation="MLDPSLAREVLGAALAGGAAFAEIFAEDRRSTSGRLDDGKIEEL
                     SSGHDRGAGVRAVAGDTTAYAYSNRLDREALVEAAESAAAAARGSDRTSVLDFTRAEP
                     AVTHEPQEPSGGVPAERKVAWLQEADDAARSAGSEVRQVIAGYGDSVQRLLIASSEGL
                     WVEEERHRLRLVVQVVAARADVLQTGFEGPAGLGGVEVLRAHPPAESGEKAARQAITM
                     LDGVPAPAGEMPVVVGPAGGGVLFHEACGHGLEADTVGKEASIYRGRLGETLGSTVVT
                     GVDDSTVDHAWGSFSFDDEGSPAKRTVVFEEGVLKSFLYDRFWARKDGAAPTGNARRQ
                     SYAHLPIPRMTNTSILPGKDDPGDIVADTPRGLYVKALGGGQVNPATGDFVFGASEAY
                     LIEDGRITTPARGANLIGNGISILRAVDAVADDFDARPGTCGKDGQGVPVTTGSPTLR
                     IARMTVGGTGG"
     gene            7131..8477
                     /locus_tag="VF097_10175"
     CDS             7131..8477
                     /locus_tag="VF097_10175"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX6263201.1"
                     /translation="MADLGDLCEVAVGAAESGEAVEAYAGQSRRTEVKARGGEVDSLA
                     RSETRGIGVRVIADGRLGYAWAADPDRDEIAGLVASAREAARHAASDEANVLPEAEAA
                     EPLPGLFREAQASLGSERKVELALELDHASTSSHPDVRKVEEVIYADVVSRVAIASTA
                     GVQAERTRTDCWCMVSALAERDGETQSGFSFAVARELDELDWRGAAAEAAERGARLLG
                     ATKPSTERLPVVLDPWAASSFLGVLAGALSAEEVQKGRSLFAGKVGDSVGAGILTLVD
                     DGRLPDGPGSTPFDDEGVPTGRTPLIEAGVLRGFLHNAQTAARGGTRSTGNAGRAGYR
                     SVPGVSPANLVVEPGDDGPEAVLAQAGRGVYVQSVTGVHSGANPVSGEFSVGATGLRI
                     EEGSLGEPLREMTIASTLQDVLGALVAIGSDLRFFPGGGAIGTPTMLVGEMTVAGA"
     gene            8545..9288
                     /locus_tag="VF097_10180"
     CDS             8545..9288
                     /locus_tag="VF097_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263202.1"
                     /translation="MRRFLSPAGLALAAFCFFLPFVSVSCNPGALEDVIGEELPTTGP
                     TPFPGGFPGGLGQEVEVTSGTGWEIALGSEPELSDTFGGLAELGGAEETTADEPWPGQ
                     AYAIAALALAVLGIVLALVLKGMPGALSGLIAGVGGGVMVFLIRTEISSWFEDELRTD
                     PQAGAILDLNFEIGWWLALAFFIVAAALAGWAMAAPRQPTEFAAPPPPAAPPGGAPPP
                     PTGPPPDGAPPPPPEAPPPTRPQEPPPPA"
     gene            complement(9292..10116)
                     /locus_tag="VF097_10185"
     CDS             complement(9292..10116)
                     /locus_tag="VF097_10185"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX6263203.1"
                     /translation="MLRALILGAVQGLTEFLPISSSGHLVLVPFVLGWEGGTGFQRGV
                     SFDVAVHLGTALAVVVYFRRELVAIISGTVRVVARRGRGDDRDQARLALLLAVGSIPA
                     AAAGLLLEGFFVELFEEPPIAALLLLVTAGLLLAGEAIHDRQGEPRRGLADLRIADAL
                     TVGAFQALAIAPGISRSGATIVAGLGRRMERDAAARFSFLLALPAIVGAGILKIPDFP
                     AGTDLGPVIGAMVVSAGAGFAAIAFLLRYLRTRTLRPFAAYCVLASAVALTIWLVR"
     gene            complement(10126..10764)
                     /gene="cpaB"
                     /locus_tag="VF097_10190"
     CDS             complement(10126..10764)
                     /gene="cpaB"
                     /locus_tag="VF097_10190"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEX6263204.1"
                     /translation="MRRKLPRSSVALALAAVVVGVGTTVALRGRLAALEARADAAGDL
                     RPAVVAAVDVTRGARIEPGHVETVEMPAALLPSTALTSERQAIGATLAGDVAEGEVLT
                     ETRVARAGPIAALVPEGFRAMGVTVAIAPGLLAPGDRVDVLAVAPGRPFAETLASGVE
                     VLAVRETSPAEASVATVVLLVTPGQASQLAAGRAGAELALTVLPAGEVTLSG"
     gene            complement(10833..11627)
                     /locus_tag="VF097_10195"
     CDS             complement(10833..11627)
                     /locus_tag="VF097_10195"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEX6263205.1"
                     /translation="MSTIGIFGGSGFYSLLEDAREVEVDTPYGKPSAPFTVGEIGGRE
                     VAFLPRHGRKHQFPPHKVPYRANLWGMKELGVERVVGPCAAGSLQAHVKPGHFVLCDQ
                     LVDRTSGREDTFYDGPETTHVSFADPYCPTMREVAFEQARDQGIEIHDRGTVVVIQGP
                     RFSTRAESAWFQSEGWEVINMTQYPEAALARELELCFLNISLITDYDVGVEGEDVEPV
                     THEAVIQVFEENIEKLKELLHRIVPELPEIRDCPCATALQGARFEP"
     gene            complement(11624..11890)
                     /locus_tag="VF097_10200"
     CDS             complement(11624..11890)
                     /locus_tag="VF097_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013127857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEX6263206.1"
                     /translation="MPTYEYECRECGYRFEVNQRISDDPLIECEVCGGPLRKLFHPVG
                     IAFKGSGFYVTDNRGSRAKAPSSDGKKPEKKAQSATSESKEKTA"
     gene            complement(11924..12499)
                     /locus_tag="VF097_10205"
     CDS             complement(11924..12499)
                     /locus_tag="VF097_10205"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX6263207.1"
                     /translation="MTDDPVKAALRAEMRRVRAAIPEGERRRRGEAIRERVLGLPEVR
                     RAGSVLVFASFGSEMPTRDLASDLHTAGKRLLLPYLSGKVMEVAEVGPDEPLAPSSYG
                     PGEPERRVRADPEGIDVVIAPGLAFDRRGNRLGYGGGAYDRFLARIRPEALVVGVAFA
                     EQVVGEVPAGPNDVPVHLVVTDREVIDARRG"
     gene            12608..>13759
                     /locus_tag="VF097_10210"
     CDS             12608..>13759
                     /locus_tag="VF097_10210"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX6263208.1"
                     /translation="MTLRARLTLFFAGIVVVPLVAATLVLQILVTQEVRRRSDARLEA
                     ASATLSTLWQERLTVAERETGRAASVAAELLPGRGTALEERIEVERSAAGLDFLVVTN
                     PQGGIIASSVGSPLFARGVTPPTAFDIVGERSVPGLLRARAPISVTGNLGDVVGGWFV
                     DTEFLRELVGPADVELVLRSGMRVLAATESSLGNLPARGPYPLSVGDGRRVTATQIRG
                     QSGEVVLVTPEEVADVHGAVWGVAVLGLLIASMLGYLLAGVIGRPVYELAEGARRVAA
                     GDLHTRVAVGDRGDIGNLANAFNQMTENLETYVTRLEESRDELRRNLDRLGATLRSTH
                     DLDAMLSVIVDTAAATLHASTAALFLLTPSGKRLRLQAVLGYEPSAVTEM"
BASE COUNT         1801 a         5063 c         5012 g         1883 t
ORIGIN      
        1 ggggaacgtc cggttcgtta ccgccgtcgg ggccgcaccc gtccccgtcc gcgccctcga
       61 cggctcgagg tgaagaccgc cctgggcgcg gggaccttca tcccctccac ctcgaactcg
      121 tccccgagct tgaggtggcg cgcgatgcgg ccgacgtctc cctgcttgtc cggcgtcacc
      181 agcgtcacgc cggtgcccgc gcggccggcg cgggcggtgc ggcccacccg gtggacgtag
      241 cccttcccgt cctcgggcgg gtcgtagttg atgacgtgag tgatgtcgtc gagatcgagg
      301 ccccgagccg ccacgtcggt ggccacgagc gcgtcgatcg atcccgcctc gaacttcgcc
      361 agcgtccgct cgcgaagctg ctggcgcatg tcgccgtgca tcgcctcggc ccggacgccc
      421 ttgctcttca gccgggtcac gaggcggtcg gccccccgct tggtccggac gaagatcagc
      481 gcgagcccgc gctcctcctt gaggaggtcc acgagcacgg gcagcttgtc gccgcccgga
      541 acgggcagga agcggtggct ggcctcgtcg accgtctcga tcagggtctc gatctcgtgg
      601 cggaccgcgt cggtcgtgta gcgcgacgcc aggaacccga cgtggccgtc gagggtggcc
      661 gagaagaaca tcgtctggcg atccttgggg acccggcgca cgatgcggtc gacctgcggc
      721 tggaagccca tgtcgagcat gcggtcggcc tcgtccagca cgaggacgcg gatcccgtcc
      781 agcttcacga gcttgcgctg agcgaggtcc tcgagccggc ccggggtggc gaccaggatg
      841 tgggccttgg cggcccggtt cgcctgctcg cggatgccca cgcccccgta ggccacggcg
      901 acccgcaggc cgcgggcctt ggcgatgggc tcgaagtcct ctcggacctg cgaggcgagc
      961 tcgcgggtcg gcacgagaac cagcgccgtg ggccgggggt ccttcggatc ggtggtctcg
     1021 acgatgggga tggcgaaggc gagggtcttg cctgaccccg tgcgggactt ggccaggacg
     1081 tcgtgcccgg ccatggcatc gggcagaacc agggtctgga tggggaaggg cgctcggatc
     1141 ccgtgggcct cgagggcccg gatcacgggc gtggaagcgc ccagcgtgcg gaaatcggac
     1201 attctgttcc tcctcgcgga tgagccgcgt gttcgaggct tcgccttgcc gcgactcgaa
     1261 ggaggaacgg gaagtgaacc ctcacgggcg acgccctgtc gccctcgcgg accaggatag
     1321 caggtcgccg gccggttccc cggagggcta cccgatcgtg accggctcgg ccggcacccg
     1381 gccgggcagg tggagctcga tcgccgtccc cttcccggcc tcgctgtcca cccggagcca
     1441 cccccccgcg gccatcgccc gctggcgcat catggcgatc cccatgtggc tgggcgacgg
     1501 gacgacgccc tcgggctcga agccgacccc gtcgtcctcg atccggagga agaggccatc
     1561 gtcggactct accgcgacgt ccacccgggt ggcccgggcg tgcttctgga tgttcgccag
     1621 cgcctcctgg aggatccggt acgcggcgga ggccgtctcg aagggcggct cctcgatcac
     1681 ccgttcgctg agccgggcct cgaggtcccc gtcctcggcc gcccgctcca gcatggcccg
     1741 gagctcggcg aggagcccga actcctcccg cggccccctg gccagctcga acgtgaggcc
     1801 gcgcagatgc tcgatcgcgg cggtgagcgt ctcctggatc cctccgatct cggagcggag
     1861 ggcgggatcc tcctgcgctt ggcgcagccc ctggagtcgc gtcgagatcg tggagagcac
     1921 ctggaggggc tcgtcgtgga tctcggaggc gatccgctgg cgctcgaggt cgcgggcctt
     1981 caccagccga ctgagcagcc ggcggcgcgc cacgaggcgc gccgaataca gcgagtcgat
     2041 cggatccgtc ccttcgtcca ccgatgtggt cacgccatgc ccccttgctc tcccgacgcg
     2101 gtccccgcac cttgtatcgg cgggggcggc ggggaaactt gatgtcgccg gatcgtccca
     2161 ccgcggtcgc cctgacggga gccggaccgg tcggcggacc gccccgcctc ggcgtcccct
     2221 gctaccctcg gcccatccca tgggcaccag gagccgcgct cgcctcgcca cgaccgcgat
     2281 cctcgcgtcc ttcggcgcgg tggccatcct ggcctcccgg ccgaactctc cgttcacgcc
     2341 gttcctccac ccgggcgacc gatctccgct gcccctccgg gagctctccc acctcctcgg
     2401 gctcgacgcg ctggaccggg acgtcgcggc cgtcgtcggg gcggccctgc tcgtggcctc
     2461 ggcgatcgcc ttcgtgtacg cggcccgggc cgcatggagg ggagacctct ccgtgtcgag
     2521 ggtgatctgg gtgggggtgg cgctccacgt cctggcgttc gtgctgccgc tgttcctgtc
     2581 ccgcgatgtc tacagctacg cgtactacgg ccggatggtt tcgcggtacg gcgtgaaccc
     2641 ctacctgttc acgcccgggc agttcctgca ggaccccttc tacctgtacg tgtcgcactt
     2701 ctggatcgac tccccctccg tgtacggacc cgcgttcagc gctctcgccg gctgggtgac
     2761 gggcgttgcc tcgagcgtcc cggcgacggt ggacgcgttc aagctcgtcg cggcggtggc
     2821 gggcgtgggg acgatgctgg ccgtcgtggc cgccgctcgg cgggtccggc ccgagcgggc
     2881 cgccttcgcc ggcctcctga tcggatggaa ccccgtgctg atcttccaca acgtcgccgg
     2941 ggggcacaac gacgcgctcg tgggcctggc cgtggccctc gccgcgctcc tcctgctgtc
     3001 ccgtcgagat gtgctggcca cgggggtcct cgtcctcggg tgcctggtga aggcgacggc
     3061 ggtgatcccg ctggcgatgc tcgtcgtggg ctcggttgcc gcccagccca aggggcgtcg
     3121 gctgcggtcg ctggcggcgc acgcggcggt gggcctcgtg gtgggcctcc cgttcgtgat
     3181 ccggttcttc tcgctggagg acccctggat gggccagctc gagctcgcca cgaggcaggg
     3241 ctggctggcc ccgtcccggt tcctcgtggt caccctccgg gcgggggtga acgccgtggc
     3301 cggggagacc gcgggggatc tcacctcggc gctcatccgc atgatcttcc ccgtggtctt
     3361 ggcgctcgtc ctggcctcga tcgggaggca cgtggctcga cgccccgaca ggatccgacc
     3421 cgagctgatc gtcgcagcga tgggatgggc cacgttgttc ggcctgatga tggccccgat
     3481 cctgctcccc tggtacgccg cgtggatggt cccgctggcg tggatgctgc cgaagccggc
     3541 gcggaacggg gcgatcttca tctccgtggc gctcgtgatc accgagctaa tgagcgagcc
     3601 cacgaggtca cctcgcgtgt gggaggtgat ggtgatctgg ctccactggg tggccacgcc
     3661 gatcgtgctc gcggtcctgg tccggctgct tctggagctc cgggcccgcc tccggatggg
     3721 gccggcggag ggggcgtccg acccgctgct gaccgagcgg ccgctcctac gctcgcggat
     3781 gctccggatc ctcgaccggg cggtgtcggg ccgcgaggaa gtagccgacg agggcgagcg
     3841 ttccggcgag ggcggcgccg ccgcttcccg cggggagcgt gagccggtcg gcggccaggc
     3901 cgcccaggat cagcacgccc aggcccgccg ccggggcggc ccccaggacg gccaggggcc
     3961 cggcgccatg gagcccccag gctgaccacc ccaggccgag gagagcgagg ccgagcgcgg
     4021 cgagcagcga gaggcccacc cacgcccagg gggagatccc ggggtcggac ccggagcggt
     4081 cggcggccgg cgcggtgaac ccctcggggg cgcggaggag gacggcatgg ggggagaggg
     4141 gttcgccgag ccggcgggcg tccgcgaacc ccggatggaa cggctctagg acgatgatcg
     4201 cggcctcgtc gagcaccggt cgggcccgct ccaggtaggc ctccgcgatg cccacgtact
     4261 cggggtcctc gctccgggtg ccgggccggc cggccaggac gtcctcggcg tcgcccacga
     4321 cgaggctcgt ctcggcgatc cgctcgggcg gcagggcggc ccggatcacg ttgccgaagc
     4381 gagcgacgtg gaaggcagcg gcgaactctt cggtgtccac cgccacgagg atgggggtgt
     4441 cctcgggcag ctcgtcgagg acgggttcga ccgacccgac gctccgaagc tcctcctcgc
     4501 tcatgaaggg ggtctgccgg taccagcccc agagcgacga ggcggctacg atggcggcgg
     4561 ccgcggcggc ggcgatccgg atccagccgc cgcgccgcat cgcgctcgcg gccgccgcgg
     4621 ccgccgccag gggcaggacg taggcgaagg tcaggagccg gcccggaggc cccacgccgg
     4681 tggcccagag gaccgccgcg ccgacgacgg tgaccgccac ccacgaggcc gccaccgaga
     4741 tcgcgtagcg gcgatgctcg cggtccacgc cgtcgagcag gcccgtcccg gccgcgccca
     4801 ggatcgccgc ggcagcgggg atcgccatcc ggcgggcgtc cccccacagc cgctctcggt
     4861 accgctcgcc gagggttccc cccatcccga tctggcggaa gacctgatcc tgcgaggtat
     4921 cccccggcgc cggcggcccg ccggccagca gccccacggc caggagcgcc cccccgcccc
     4981 cggcggcggc gcccacgctg atccgcatcg ccgccgtgtc gaggggccgg acgccggctc
     5041 ggatccggcg cagtgcgtcg ggggcgagca ggatcacggc gcccgtaacg atccccagcg
     5101 ccacggcgag gaagagcgag tgcgcgaccc cggccgccgc gagggccacc gctccgaagc
     5161 cgaccccccg ccaggaccgg ccggcgacgg cgaaggccgc cagggccgtc aggaagagcg
     5221 ccgccaggac caggttcgcc atccacccgg ccgccagcag gccggcgaag gcccccacga
     5281 gcagggaggt cgtcccgaac cggacccggt ccggtcccag gacggcctcc gcgaccgcgc
     5341 cccccgccag cccgaccgcc gccgccaggg cgggctccag cccggcgacg atccggagcg
     5401 ggtcggtgac cagcagcgag gtgagcgcca ggatcagggc gggcaccccg ggccggcgga
     5461 ccgcctccag gccctccacc tccacgaacc gagaccacca cacgtagacg ggcccgtccg
     5521 ggcccagcgg caggcggtgc ccttcgagga gggggtgggc caggaaaacg ccgaggagga
     5581 ggagcgcgac gcctccggcc caggtgagcg ccgccggcca cgcggcgcgt ggccggcggc
     5641 gcgagccgcg ggtgggcgcg cgccgggcgg ccgggacccg gctcatggcc gccacggtac
     5701 cgcctgggca ccggccgggc cacactcgag ggccctgcta ggatgcggcc tcgtgctcga
     5761 cccatcgctg gcccgagagg ttctcggggc cgccctggcc ggcggcgccg cgttcgcaga
     5821 gatcttcgcc gaggaccgcc gctccacgtc ggggcgcctg gacgacggca agatcgagga
     5881 gctctcgagc ggccacgacc ggggggcggg cgtgcgggcg gtggcggggg acaccaccgc
     5941 ctacgcctac tccaaccgcc tggaccggga ggcgctcgtg gaggcggccg agtccgccgc
     6001 cgccgcggcc cgcggctcgg accggacttc ggtgctcgac ttcacccggg cggagcccgc
     6061 ggtgacgcac gagccacagg agccctccgg cggcgtcccc gctgagcgca aggtggcctg
     6121 gctccaggag gccgacgacg ccgcgcgctc ggctggctcc gaggtccgcc aggtgatcgc
     6181 cggctacggc gactcggtgc agcggctgtt gatcgcgagc tccgagggcc tctgggtaga
     6241 ggaggagcgt caccgcctcc gcctcgtcgt gcaggtcgtg gcggcgcgcg ccgacgtcct
     6301 ccagaccggc ttcgaggggc ccgccggcct cgggggcgtg gaggtcctgc gggcccatcc
     6361 ccctgcggag tcgggggaga aggccgcccg tcaggcgatc acgatgctgg acggcgtccc
     6421 cgcacctgcc ggcgagatgc cggtggtggt gggccccgcc gggggtggcg tcctcttcca
     6481 cgaggcgtgc gggcacgggc tggaggccga cacggtggga aaggaggcct cgatctaccg
     6541 gggccgcctc ggcgagacgc tcggctccac ggtcgtcacg ggcgtcgacg actccacggt
     6601 cgaccacgcc tggggctcgt tctcgttcga cgacgaaggt tcccccgcca agcgcacggt
     6661 ggtgttcgag gagggcgtcc tgaagagctt cctctacgac cgcttctggg ctcgcaagga
     6721 cggcgccgcg ccgacgggca acgcccgccg ccagtcctac gcgcatctcc cgatccctcg
     6781 gatgaccaac acctcgatcc ttcccgggaa ggacgacccc ggcgacatcg tggccgacac
     6841 cccgcgtggc ctatacgtga aggcgctggg cggcgggcag gtcaaccccg cgacgggcga
     6901 cttcgtcttc ggagcatcgg aggcctacct gatcgaggac gggaggatca caaccccggc
     6961 ccggggcgcg aacctgatcg gcaacggcat ctccatcctc cgggcggtgg acgccgtggc
     7021 ggacgacttc gacgcccgcc cgggcacctg cggcaaggac ggccagggcg tcccggtgac
     7081 gaccggcagc cccaccttgc ggatcgcgcg gatgactgtg ggagggaccg gtggctgacc
     7141 tcggggacct gtgcgaggtc gccgtgggag ccgcggagag cggcgaggcc gtcgaagcct
     7201 acgccggcca gtcccgcagg accgaggtga aggctcgcgg gggcgaggtc gactcgctgg
     7261 ctcgctcgga gacccggggg atcggcgtcc gggtgatcgc ggacggccgg ctcgggtacg
     7321 cgtgggccgc cgatccggat cgggacgaga tcgccggcct cgtggcctcg gctcgggagg
     7381 cggcccggca tgcggcctcc gacgaggcga acgtcctccc cgaagcggag gccgccgagc
     7441 cgctccctgg gctgttccgc gaagcacagg cctctctggg gtccgagcgc aaggtggagc
     7501 tcgccctcga gctcgatcat gcctcgacct cctcgcatcc cgacgtccgg aaggtcgagg
     7561 aggtgatcta cgccgacgtc gtgtcgcgcg tggcgatcgc ctccaccgca ggcgttcaag
     7621 ccgagcgcac ccgcaccgac tgctggtgca tggtctcggc gctggccgag cgcgacggcg
     7681 agacgcagtc ggggttctcc ttcgccgtgg ccagggagct cgacgagctg gactggcgag
     7741 gcgccgcggc cgaggccgcc gagcggggag ctcgtttgct gggcgcgacg aagccctcca
     7801 ccgagcggct ccccgtcgtc ctcgacccgt gggcggcctc ctcgttcctc ggggtgctgg
     7861 cgggcgcgct ctcggccgag gaggtgcaga agggacggtc gctcttcgcc ggcaaggtcg
     7921 gggattctgt gggggccggg atcctcacgc ttgtggacga cggccgcctc cccgacgggc
     7981 ccgggtcgac tcccttcgac gacgagggcg tgccgacggg tcgcacgccg ctgatcgagg
     8041 cgggggtgct ccgcggcttc ctccacaacg cgcagaccgc ggcccgggga ggcacgagat
     8101 ccacggggaa cgcggggcga gcgggctacc ggagcgtccc gggggtctcg cccgcgaacc
     8161 tcgttgtgga gccgggtgac gatggccctg aggccgttct ggcccaggcg ggcaggggcg
     8221 tgtacgtcca gagcgtcacc ggcgtccatt ccggcgccaa cccggtcagc ggcgagttct
     8281 cggtcggggc gacggggctc cgtatcgagg aggggtccct cggggagccg ctccgcgaga
     8341 tgacgatcgc cagcacgctc caggacgtcc tgggggcgct cgtcgccatc gggtcggatc
     8401 tgcgcttctt ccccggagga ggcgcgatcg ggacgcccac gatgctcgtc ggcgagatga
     8461 cggtggcggg cgcctgaggc gccccgtgct tgaccccctc ccccgcatcg catcaaatgg
     8521 acccacccga cgggaggtgt ccctatgagg aggttcttgt cgcccgcggg ccttgcgctc
     8581 gcggccttct gcttcttcct gcccttcgtc tcggtgtcgt gcaacccggg cgcactggag
     8641 gacgtgatcg gggaggagct tcccaccacc ggacccacac cgttccccgg tgggttcccc
     8701 ggagggctgg gtcaggaggt cgaggtcacc tctgggaccg ggtgggagat cgcgctcggc
     8761 tccgagccgg agctctcgga cacgttcggc gggctggccg agctgggcgg cgccgaggag
     8821 acgaccgcgg acgagccctg gcccggccag gcctacgcca tcgcagcgct ggccctcgcc
     8881 gtcctgggga tcgtgctggc gctggtgctc aaggggatgc cgggcgctct gtcgggtctg
     8941 atcgccggcg tgggaggcgg ggtgatggtc ttcctgatcc ggaccgagat cagcagctgg
     9001 ttcgaggacg agctccggac cgacccgcag gcgggtgcga tcctggatct caacttcgag
     9061 atcgggtggt ggctggccct ggccttcttc atcgtcgcgg ccgcgctcgc cgggtgggcc
     9121 atggccgccc cgaggcagcc cacggagttc gcggctccgc ccccacccgc cgcgccgccg
     9181 ggaggggctc cgccgccgcc gaccgggccg ccgccggacg gggctccgcc gccgcctccg
     9241 gaagcgccgc cacccacgag gccccaggag ccgccgccgc cggcctgagc gtcagcgcac
     9301 gagccagatc gtgagggcga cggccgaggc cagcacgcag taggcggcga agggccggag
     9361 ggtcctcgtc cgcaggtacc ggaggaggaa ggcgatcgcg gcgaacccgg ccccggccga
     9421 gacgaccatc gcgccgatca ccggcccgag atcggtcccc gccgggaagt cggggatctt
     9481 caggatgccc gcccccacga tggccggcag cgccaggagg aacgagaatc gggcggcggc
     9541 gtcccgctcc atccgccgtc cgaggcccgc cacgatcgtg gcgcccgagc ggctgatgcc
     9601 cggagcgatg gccagcgcct ggaaggcgcc taccgtgagc gcgtccgcaa tccgcaggtc
     9661 ggccagcccc ctccggggct cgccctgccg atcgtggatc gcctcgcccg ccagcaggag
     9721 gcccgccgtg accaggagga ggagggccgc gatcggcggc tcctcgaaca gctcgacgaa
     9781 gaagccctcg aggaggaggc ccgccgcggc cgcggggatc gaccccaccg ccagcaggag
     9841 ggccagccgc gcctggtccc gatcgtcccc gcggccgcgg cgggccacca cccgcacggt
     9901 tcccgagatg atggccacca gctcacgccg gaagtagacg acgaccgcca gggcggtccc
     9961 cagatgaacg gcgacgtcga aggatacccc tcgctggaac ccggttcccc cctcccatcc
    10021 caggacgaag ggcacgagca cgaggtgtcc ggaggacgag atcgggagga actccgtcag
    10081 cccctgtacc gcccccagga tcagggctcg aagcatgcgg ggagtctagc cgctcaaggt
    10141 gacctctccc gccggcagca ccgtcagggc cagctcggcc ccggcccggc cggccgccag
    10201 ctgggaggcc tgccccggcg tgaccaggag cacgaccgtg gccacggacg cctcggccgg
    10261 cgacgtctcc cggaccgcca gcacctccac ccccgaggcc agtgtctccg cgaacggcct
    10321 ccccggagcc accgccagca cgtccacgcg gtcgccgggg gcgagcaaac cgggcgcgat
    10381 cgccaccgtc acccccatcg ctcggaaccc ttcggggacg agcgcggcga tcgggccggc
    10441 gcgggccacc cgggtctccg tcaggacctc gccctcggcc acgtcgcccg cgagcgtcgc
    10501 cccgatcgcc tgacgctcgg acgtcagcgc ggtggatggg aggagcgccg cgggcatctc
    10561 cacggtctcc acgtgtccgg gctcgatccg tgctccccgg gtcacgtcca cagcggccac
    10621 caccgccggc cgcaggtcgc cggccgcgtc ggccctcgcc tccagggcgg ccagccgtcc
    10681 cctgagcgcg accgtggtcc ctaccccgac caccaccgcc gccagcgcga gcgcgacgga
    10741 cgaccggggg agcttcctgc gcatgagccc tcctccgagg gggaggctgg gagacgtccg
    10801 gggagcctag cgccccccac cgacacgaac gcctacggct cgaacctggc gccttggagc
    10861 gcggtggcgc aggggcagtc gcggatctcg gggagctcgg gaacgatccg gtgcagcagc
    10921 tccttcagct tctcgatgtt ctcctcgaac acctggatca cggcctcgtg ggtcacgggc
    10981 tcgacgtcct cgccctccac ccccacgtcg tagtcggtga tcagcgagat gttgaggaag
    11041 cacagctcca gctctcgggc cagcgcggcc tcggggtact gggtcatgtt gatgacctcc
    11101 cacccctcgg actggaacca ggccgactcc gcgcgtgtcg agaagcgggg cccctggatc
    11161 accaccaccg tgccccggtc gtggatctcg atcccctggt ccctcgcctg ctcgaacgcc
    11221 acctcccgca tcgtggggca gtaggggtcg gcgaacgaga cgtgggtcgt ctcgggtccg
    11281 tcgtagaagg tgtcctcccg cccgctcgtc ctgtccacga gctggtcgca cagcacgaag
    11341 tggcccggct tcacgtgcgc ctggagtgag cccgccgcgc acggccccac cacccgctcc
    11401 acccccagct ccttcatccc ccagaggttc gcccgatacg gcaccttgtg gggcgggaac
    11461 tggtgcttcc gcccgtgccg agggaggaac gccacctcgc gccccccgat ctcgcccacc
    11521 gtgaacggcg ccgagggctt cccgtagggc gtgtccacct cgacctcgcg agcatcctcc
    11581 agcagggagt agaagccgga cccgccgaag atcccgatcg tgctcaggcc gtcttctcct
    11641 tcgactccga ggtcgcgctc tgggccttct tctcaggctt cttgccgtcg ctcgagggcg
    11701 ccttggccct ggacccgcgg ttgtcggtca cgtagaagcc cgagcccttg aacgcgatgc
    11761 cgaccgggtg gaagagcttg cgcagggggc cgccgcagac ctcgcactcg atgagcggat
    11821 cgtccgagat gcgctggttc acctcgaagc ggtaaccgca ctccctgcac tcgtattcgt
    11881 aggtcggcat ggcccagcga ttataggggg cggagcgagc cgctcacccg cgtcgagcgt
    11941 cgatcacctc gcggtcggtc accacgaggt gcacggggac gtcgttggga ccggccggga
    12001 cctcccccac gacctgctcc gcgaaggcca cccccaccac cagggcctcc ggacggatcc
    12061 gggccaggaa gcgatcgtac gccccgccac cgtagcccag ccggttcccc ctgcggtcga
    12121 acgcgagccc cggggcgatc accacgtcga tcccctcggg gtcggcgcgc accctccgtt
    12181 cgggctcccc cgggccgtag gagctcgggg cgagcggttc gtccggaccc acctccgcga
    12241 cttccatcac cttcccggag aggtagggta ggagtaggcg cttcccggct gtgtggagat
    12301 cggaggcgag gtcccgggtc ggcatctccg acccgaacga ggcgaagacg agaacggacc
    12361 cggcccgccg gacctcgggc aggccgagga cccgctcccg gatcgcctct ccccgccgac
    12421 gacgctcgcc ctccgggatc gccgcccgca cgcggcgcat ctccgcacgg agggcggcct
    12481 tgacgggatc gtcggtcacg gtcgctcctg attaagtcgt gggtgagcag gtgccgacaa
    12541 cgaaagggag agaatcgatg cgcgcgcgtc gctcatcccg ggaagccaac gcgcgagggg
    12601 cccagccgtg accctgcgcg ctcgcctcac gctgttcttc gccggcatcg tcgtcgtccc
    12661 cctcgtcgcg gccacgctcg tcctccagat cctggtcacc caggaggtgc gccgtcggtc
    12721 cgacgcccgg ctcgaggccg cctccgccac gctctcgacc ctctggcagg agcggctgac
    12781 cgtggccgag cgggagaccg gccgtgcggc gtccgtggcg gccgagctgc tccccggccg
    12841 cggcacggcg ctcgaggagc ggatcgaggt ggagcgctcc gccgcgggtc tcgacttcct
    12901 cgtcgtcacg aacccccagg gcggcatcat cgcatcgtcg gtgggctccc cgttgttcgc
    12961 ccgtggggtt actcccccca cggccttcga catcgtgggg gagcgcagcg tgccggggct
    13021 gctccgtgcc cgggctccga tctctgtgac gggcaacctg ggggacgtcg tcgggggctg
    13081 gttcgtggac acggagttcc tgagagagct cgtagggccg gccgacgtcg agctggtcct
    13141 gcgttccggg atgcgggtgc tggccgcgac ggagtcgtcc ctcgggaacc ttcccgcccg
    13201 ggggccgtac cccctctccg tcggtgatgg ccgtcgtgtc acggcgactc agatccgtgg
    13261 gcagagcggc gaggtcgtgc tcgtgacgcc cgaggaggtg gcggacgtcc acggcgcggt
    13321 ctggggggtc gccgtgctcg ggctgctgat cgcgagcatg ctgggctacc tgctggcggg
    13381 cgtgatcggg aggccggtct acgagcttgc cgagggcgcc cgtcgggtcg ccgccggaga
    13441 cctgcacacc cgcgtggcgg tcggggaccg cggcgacatc gggaacctcg ccaacgcctt
    13501 caaccagatg accgagaacc tcgagaccta cgtgacccgg ctggaggagt cgcgcgacga
    13561 gctccgccgg aacctcgatc gcctcggggc cacgctccgg tccacccacg acctcgacgc
    13621 gatgctgagc gtgatcgtgg acaccgctgc cgcgacgctg catgcgtcca ccgccgcgct
    13681 gttcctcctc acgccctcgg ggaagcggct gcggctccag gcagtcctgg ggtacgagcc
    13741 gtcggcggtc accgagatg
//
LOCUS       DASUTZ010000239        14381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1902026, whole genome shotgun sequence.
ACCESSION   DASUTZ010000239 DASUTZ010000000
VERSION     DASUTZ010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14381
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1902026"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VF097_10215"
     CDS             complement(<1..327)
                     /locus_tag="VF097_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX6263209.1"
                     /translation="MAQPAEQVKGRQKTGGQKGLVIREYPLSRTRNIGIMAHIDAGKT
                     TTTERILYYTGRTYKVGEVHEGTAVMDWMVQEQERGITITSAATTTKWKDHWINIIDT
                     PGHVDFT"
     gene            complement(413..883)
                     /gene="rpsG"
                     /locus_tag="VF097_10220"
     CDS             complement(413..883)
                     /gene="rpsG"
                     /locus_tag="VF097_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX6263210.1"
                     /translation="MPRRGAASKRATVPDPIYQNPLVTQLINKVMRDGKKSLAERLVY
                     DALERISEQTANDPVLTLKKAVENTRPLLEVRSRRVGGATYQVPVEVKPQRGTTLALR
                     WLVGFARQRREPTMAARLVGEIMDASQGQGSAVKRREDIHKMADANRAFAHYRW"
     gene            complement(885..1256)
                     /gene="rpsL"
                     /locus_tag="VF097_10225"
     CDS             complement(885..1256)
                     /gene="rpsL"
                     /locus_tag="VF097_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003854221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX6263211.1"
                     /translation="MPTTQQLVRRGRKEPKAKTKSPALKGSPQRRGVCTRVYTTTPKK
                     PNSALRKVARVRLTSGVEVTAYIPGIGHNLQEHSIVLVRGGRVKDIPGVRYKVIRAAL
                     DTAGVSDRKKARSKYGAKRGS"
     gene            1532..2287
                     /locus_tag="VF097_10230"
     CDS             1532..2287
                     /locus_tag="VF097_10230"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX6263212.1"
                     /translation="MEELREAALRGVLEGGRVVREAALEGTRTKGAGDYVTEVDHEAE
                     RVIATRLEEGTGIGVRGEEHGGEAAGRHWLVDPVDGTTNLVHGFPVVGVSVALIEEGR
                     PLVGAVHAPHLGATWTAVRGGGAERDGRPLAVSSRQIGEAIVATGFPFRHKNRLPEYL
                     RVFVPALHRFEDLRRPGAASLDLAWVAEGTFDGFFELGLSSWDVAAGALLIEEAGGLV
                     SDWAGGDGYLGGDILAGNPEVHAALLELAQALA"
     gene            complement(2272..3009)
                     /locus_tag="VF097_10235"
     CDS             complement(2272..3009)
                     /locus_tag="VF097_10235"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019436111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6263213.1"
                     /translation="MSDYEPSTYGDAIADVYDELHEWHLDTDAAIEALARLAGEGPVL
                     ELGVGTGRIAVPLAERGIEVHGIDASESMVEEMRRKPGGDRVRVAIGDFAGVEAPADA
                     YTMVFVVFNTLFALPSQEDQVRCFAGVAERLAEDGIFVVEAFVPDVSRFDRGQRVDVS
                     RVEPDRVVLDVSMHDPVAQRVVSQHLLLGEGVVRQIPVELRYAWPSELDLMARLAGLQ
                     IRSRHGGWREEPFTATGSRHVSIYARA"
     gene            complement(3264..6992)
                     /locus_tag="VF097_10240"
                     /pseudo
     CDS             complement(3264..6992)
                     /locus_tag="VF097_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
     gene            complement(7012..10461)
                     /gene="rpoB"
                     /locus_tag="VF097_10245"
     CDS             complement(7012..10461)
                     /gene="rpoB"
                     /locus_tag="VF097_10245"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX6263214.1"
                     /translation="MSLPNAVTGRERVSFAKLQEPQELPDLIAIQRESFDWLITEGLR
                     EVFDEISPIEDYTETYELRFGDFWFEDVKHSVDECKDKDMTYSAGLHVQAEFINKQTG
                     EIKSAPVFMGEFPMMTERGTFIINGTERVVVSQLVRSPGVYFDKQLDKTADKDIYIAK
                     IIPARGAWLEFEVDKKELVGVRIDRKRRQNVTVLLKALGWSSDEIMELFDNAPTIAAT
                     LEKDGLETPEEALEDIYRKLRPGEPPTAESARNLLENLFFNPKRYDLAKVGRYKVDKK
                     LGEVDDLLKTQLKRRYETLREQDKPERKEFEQPRWRVFSHPYGNERGAAPFKSVLTYE
                     DMVKTIRYLVRLHAGQEGFDTDDIDHFGNRRLRSVGELIQNQIRIGLSRMERVVRERM
                     TTQDVEAITPTTLVNIRPVVGSIKEFFGTSQLSQFLDQTNPLAGLTHKRRLSALGPGG
                     LSRERAGFEVRDVHPSHYGRMCPIETPEGPNIGLIGSLSSFGRMNDFGFVETPYRKVE
                     KGKVSDQIDYLTADEEDLFVIAQANAELDRNGRFVSDRVLVRRKGGEVDYVPSSEVDY
                     MDVSPKQIVSVGAAMIPFLEHDDANRALMGANMQRQAVPLLRAEAPLIGTGVELRAAM
                     DAGDVVMAEKAGTVEEVSADQVVVKERDGNLRPYKLQKYRRSNQGTCINQRPVVTEDD
                     RVAAGQIIADGPSTDQGEMALGKNLLVAFMSWEGHNYEDAIVISERLAKEDVLTSIHI
                     EEHEVDARDTKLGPEEITRDIPNVSEEILKDLDERGIVRVGAEVTTGDYLVGKVTPKG
                     ETELTPEERLLRAIFGEKAREVRDTSLKVPHGESGKVIGVRVFTREDGDELSPGVNQL
                     VRVYVAQKRKISDGDKLAGRHGNKGVIAKVLPEADMPFLPDGTPVDIILNPLGVPSRM
                     NLGQVLETHLGWAAHQGFRSNGREHEGPAFVSSPVFDGAREDEIKEALATAKPNKDGD
                     RLVDEHGKAVLYDGRTGRPFEQPVTVGYIYILKLLHLVDDKIHARSTGPYSMITQQPL
                     GGKAQFGGQRFGEMEVWALEAYGAAYALQELLTIKSDDILGRVKVYEAIVKGENIPEP
                     GIPESFKVLIKEMQSLALNVEVLSAEGQEIEMRETDDDVFRAAEELGIDLSRREPAGA
                     DFE"
     gene            complement(10619..11008)
                     /gene="rplL"
                     /locus_tag="VF097_10250"
     CDS             complement(10619..11008)
                     /gene="rplL"
                     /locus_tag="VF097_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX6263215.1"
                     /translation="MAKLSGDDLLEAFKGMTLLELSEFIKQFEETFDVEAASMAAPVA
                     VAGAPGGDAGAAAEEKDEFDVILSAAGDKKIQVIKEVRGLTNLGLKEAKELVDGAPKP
                     VLEKVNKETAEQAKEKLEAAGGTVELQ"
     gene            complement(11009..11647)
                     /gene="rplJ"
                     /locus_tag="VF097_10255"
     CDS             complement(11009..11647)
                     /gene="rplJ"
                     /locus_tag="VF097_10255"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX6263216.1"
                     /translation="MPNQQKVEKVEEIRERLRSAQAAYFADYRGLSVPDIGEVRTALL
                     EAEAAFTVLKNTLTRLAVRDLEMSELESFLEGPTAVTFVQGDIVAGAKALVDATKRFP
                     VLEVKGGLAEGRVLGADEIRALAALETREEMLAKVAGLLSMHMRKAAYLMQALQSRFA
                     SLLSAYAEKLEEEAPAEKAPSEDEAGGGDEQASAEQAEEETEEAQDEEQEEG"
     gene            complement(11805..12515)
                     /gene="rplA"
                     /locus_tag="VF097_10260"
     CDS             complement(11805..12515)
                     /gene="rplA"
                     /locus_tag="VF097_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX6263217.1"
                     /translation="MGGKRYTDAMRSINREQLYTPADALRILHELPKAKFDETVELSF
                     RLGVDPRKAEEMVRGTVSLPHGTGQSVRVAAFAVGDKAREAQEAGADVVGGEDLLETV
                     LDGTIEFEAAVATPDMMPIVGKAGRILGPRGLMPNPKAGTVTEDIGKAVQDIKGGKLE
                     YRVDKAGNLHLIIGKRSFELQALSENYLTVVDEVLRAKPAGAKGRYIRSVTLSSTMGP
                     GIRIDPSKIKDVTESVPA"
     gene            complement(12515..12952)
                     /gene="rplK"
                     /locus_tag="VF097_10265"
     CDS             complement(12515..12952)
                     /gene="rplK"
                     /locus_tag="VF097_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX6263218.1"
                     /translation="MAQRPGKKVLAVVKLQIRAGQATPAPPVGTALGQHGVNIMEFCK
                     AYNEATQAQMGQMIPVELTIYEDRSFTFVTKQPPAAELLKQAARVEKGSGEPNREKVG
                     QVSRSQVRQIAETKMKDLNARDLENAMRIIEGTARSMGIEVVG"
     gene            complement(13004..13975)
                     /gene="nusG"
                     /locus_tag="VF097_10270"
     CDS             complement(13004..13975)
                     /gene="nusG"
                     /locus_tag="VF097_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010315508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX6263219.1"
                     /translation="MDEFPENDEQTQDASDREASEAAERELTDEAMPTRGEDEAPPAG
                     EGGMPEEQDVLPSEQAQEASLTGVEEATEQQREAVVAEMAEQAAEATDEDAAQAAEEA
                     AVQTVEEPEEPAEPAEHPTEEAPAPDQEVAAPEPAAEAPSDAFSGPGDWYVIHTYAGY
                     ENKVKTNLESRIQTMQMQDKIFSVYIPMEDVMEIKGGRKQVVQKKVFPGYLLVRMEYD
                     DDSWYVVRNTPGVTGFVAAGTGQKPTPLPRHEVEKFLAPQKEEAKPQFRLEFEEGDVV
                     RIIAGPFADFNGTISEINVDQSKVKVMVNIFDRETPVELSFDQVARV"
     gene            complement(13986..14258)
                     /gene="secE"
                     /locus_tag="VF097_10275"
     CDS             complement(13986..14258)
                     /gene="secE"
                     /locus_tag="VF097_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX6263220.1"
                     /translation="MQRQQRAGGERPSAPARRRQAVEQKKRERTGFRQFLREVRQELN
                     KVNWPDRRQMLIYTTVVLVTVVVLTSFVFALDFVIGKGIFTVFGIE"
     gene            complement(14301..14374)
                     /locus_tag="VF097_10280"
     tRNA            complement(14301..14374)
                     /locus_tag="VF097_10280"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14338..14340),aa:Trp,seq:cca)
BASE COUNT         1935 a         5156 c         4682 g         2608 t
ORIGIN      
        1 ggtgaagtcc acgtggcccg gcgtatcgat gatgttgatc cagtggtcct tccacttggt
       61 ggtggtggcg gcggaggtga tcgtgatccc ccgctcctgc tcctggacca tccagtccat
      121 gacggcggtc ccctcgtgga cctcgccgac cttgtaggtc cggcccgtgt agtagaggat
      181 ccgctcggtg gtcgtggtct tacccgcgtc gatgtgcgcc atgatcccga tgttccgggt
      241 cctggacagg gggtattccc ggatgaccag gcccttctgg ccgcccgtct tctgccgccc
      301 cttcacctgt tccgccggct gtgccatgtc catgttcctc gcggggacgg ccactacgcc
      361 ctcccctcgc ctcgggcccg gtcgcccggg cctcgcggat ggccctccac gatcaccacc
      421 tgtagtgcgc gaacgccctg ttcgcgtcgg ccatcttgtg gatgtcctcc cggcgcttca
      481 ccgccgagcc ctgcccctgg gaggcgtcca tgatctcgcc cacgaggcgc gccgccatcg
      541 tgggttcccg ccgctggcga gcgaacccca ccagccaccg gagcgcgagg gtcgtccccc
      601 gctgcggctt cacctcgacg ggcacctggt aggtcgcgcc gcccacccgg cgggagcgca
      661 cctccagcag cggccgggtg ttctcaaccg ccttcttcag cgtgaggacc gggtcgttcg
      721 cggtctgctc gctgatccgc tcgagcgcgt cgtagaccag gcgctcggcg aggctcttct
      781 tcccgtctcg catcaccttg ttgatgagct gggtcaccag ggggttctgg tagatcgggt
      841 cgggcacggt ggcccgcttc gaggcggctc ctcgtctggg cacctcagct ccccctcttg
      901 gcgccgtact tggagcgcgc cttcttccgg tcggagacgc ccgcggtgtc gagggccgcc
      961 cggatcacct tgtagcggac gcccgggatg tccttcaccc ggccgccccg caccaggacg
     1021 atcgaatgct cctggaggtt gtgcccgatg cccgggatgt aggccgtgac ctcgacgccg
     1081 ctggtgaggc ggacgcgcgc caccttccga agggccgagt tcggcttctt cggcgtcgtc
     1141 gtgtacacgc gcgtgcacac gcctcggcgc tgaggggaac ccttgagcgc cgggctcttg
     1201 gtcttcgcct tgggctcctt ccggccccgg cgaaccagct gctgggtcgt cggcatacct
     1261 cacccatcct cgtcttcctc gaagtgaccc aggagccgct cagaagccgg ccgacagacc
     1321 ggtcccaggg ccacggtcgc gccgaaggcc cttcggttct cctggggttg cgagacgaac
     1381 catggcccgg gcggcgcccg gaccacaatc gccaacctta taaccgtgcc tcaagactgt
     1441 caatcgagcg gcagggccgc tcgagacgtg tcccagtata cgcggccccg agcccctccg
     1501 tccggccgcc cggcctgctt gaatccaccc catggaggag cttcgggagg cggccctgcg
     1561 gggggtgctg gaagggggac gggtcgtcag ggaggcggcg ctcgagggga cccggacgaa
     1621 gggggcgggc gactacgtca ccgaggtcga tcacgaggcc gagcgggtga tcgccacgcg
     1681 cctcgaggag gggacgggga tcggggtgcg gggagaggag cacgggggcg aggccgcggg
     1741 gcgtcactgg ctcgtggatc cggtggacgg cacgacgaac ctcgtgcacg gcttcccggt
     1801 cgtgggggtc tccgtcgccc tgatcgagga gggccggccg ttggtgggcg cggtgcatgc
     1861 cccccatctc ggcgccacgt ggacggcggt ccggggcggg ggagccgagc gagacgggcg
     1921 gcccctggcg gtctcgtccc ggcagatcgg cgaggcgatc gtggccacgg gcttcccctt
     1981 ccgccacaag aaccgcctcc ccgagtacct gcgcgtgttc gttcccgccc tgcaccggtt
     2041 cgaggacctg cggcgaccgg gagcggcctc gctcgatctg gcgtgggtcg cggaggggac
     2101 cttcgacggc ttcttcgagc tcggcctgtc ctcctgggac gtcgcggccg gggccctgct
     2161 gatcgaggag gcgggcgggc tcgtctcgga ctgggcgggc ggcgacgggt atttgggcgg
     2221 agacatcctg gctgggaacc ccgaggtcca cgccgccctg ctcgagctgg ctcaggccct
     2281 cgcgtagatc gagacgtgcc gagagccggt cgcggtgaag ggctcctccc tccagccgcc
     2341 gtggcgggag cggatctgca gccccgcgag ccgcgccatg aggtcgagct cggagggcca
     2401 cgcgtaccgg agctccaccg ggatctgccg aaccaccccc tcgcccagca gcagatgctg
     2461 ggacacgacg cgctgggcga cggggtcgtg catcgagacg tcgagcacga cgcggtcggg
     2521 ctcgaccctg gagacgtcca cgcgctggcc acgatcgaat cgtgagacgt cggggacgaa
     2581 cgcctccacc acgaagatcc cgtcctcggc cagtcgctcg gccacgcccg cgaagcacct
     2641 gacctggtcc tcctgcgagg ggagggcgaa gagcgtgttg aagacgacga acaccatcgt
     2701 gtaggcgtcc gcgggcgcct cgaccccggc gaagtccccg atcgctaccc ggacgcggtc
     2761 gccgccgggc ttccggcgca tctcctcgac catcgattcg gacgcgtcta tcccgtgaac
     2821 ctcgatcccc cgctcggcca gggggacggc gatcctccca gtgcccacgc ccagctcgag
     2881 caccggaccc tcccccgcca gccgcgccag cgcctcgatc gcggcgtccg tgtccaggtg
     2941 ccactcgtgg agctcgtcgt agacgtcggc gatggcgtcg ccgtaggtgg agggctcgta
     3001 gtcgctcatg gcgggaccag cctacccaag ccgaggggcc cgggcgcgcg ctctcgcgcg
     3061 cgcccgggcc cctcgcacga tccggttcag gcctcctcgc ccacctcgga cgggacttcc
     3121 ttgcccgcgc cctgcccgtc gccctcccca aagaggacac ggcgagcctc ctcctccgtc
     3181 atctcgccct cctgagcggc accctcctcg ggcgcctgct ggcgggccag ccagtactcc
     3241 gggatggcat cggggttgat ccgcacctgg aggttccggt agcggttcat gccggtcccc
     3301 gcggggatca gcttcccgat gatgacgttc tccttgaggc cgagcagcgg gtcggacttg
     3361 gcgctgatcg ccgcgtccgt gagcacccgc gtggtctcct ggaacgacgc cgccgagagc
     3421 cacgactcgg tcgccagcga cgccttggtg atccccatca gcatcggccg cgccgacgcc
     3481 ggctcgccgc cgccctccac gacctcggcg ttcttctcct cgaagcgccg gcggtcgacg
     3541 agctcgccgg ggaggaactc ggtgtctccc ggctccagga tgtgtacctt ccgcagcatc
     3601 tgccggatga tcagctcgat gtgcttgttg tggatcgtca cgccctggct ccgatagacc
     3661 tgctggacct cctccacgag gtggcgctgc acggcgccta ccccctcgac ccgcagcttc
     3721 tcgtgagggt tcaccgaccc ctcggtgagc cgctggccga cctcgacgac gtccccgtcg
     3781 gtcacggtca gcctcgcccg cagtgagacc gcgtactgca cctgctcgcc gtcgtcggac
     3841 gtcaccgtga tcctgcggac gcccttctcc tcgtcctcct cgatctcgac cttccccgag
     3901 agctcggtga tctgggcctc gcccttgggg cgccgggcct cgaagagctc gacgacgcgg
     3961 ggcaggccgt gcgtgatgtc ctcgccggcc acccctccgg tgtggaaggt ccgcatcgtg
     4021 agctgggtcc ccggctcgcc gatcgactgc gcggcgatga tccccaccga ctcgccgatc
     4081 tcaaccaggc ggcccgtggc caggcttcgc ccgtagcaga ggcggcagac gccgtagacc
     4141 gcgtcgcagg tgaggacgga gcgggccgag atctccgaga cgcccgcgtc gaccagcgcc
     4201 ttgatcgtct cgtcggtcag ctcgtcgccg gccgaggcca ccttggagcg gttgacccgg
     4261 acgtcctcgg ccaggatgcg gccgagcaag cgagtctcga ggtgctgata gggcacccgt
     4321 tctccgtccg gatcctcgta ggtcacctcc agggtcatcc ccttgtccgt cccgcagtcg
     4381 tcctcgcgga cgatgacctc ctgcgcgacg tccacgagcc ggcgggtcag gtatccggag
     4441 tcggccgtcc gcagggccgt atccgccagc cccttccggg ctccgtgcgt cgcgatgaag
     4501 tactcgagca cggtcaggcc ctcgcggaag ttcgacttga tcgggcgcgg gatgatctcg
     4561 cccctcgggt tggccacgag gcctcgcatc cctgcgatct ggcggatctg ggtgatgttc
     4621 ccgcgggccc ccgagtgagc catcatgaag atggggttga acttgtcgaa gctcgcctcc
     4681 atctcgtcgc ggacctcgtc agtggcccgg gtccagatct cgatcagctc ctggcgccgc
     4741 tcgtcgtcgg tgatgatccc cttccggtac tgcgactcga ccttctgggc ctcgcgctca
     4801 tagccatcga ggatcttctg cttcgactgg ggggtcgtca cgtcgtccac gccgatcgtg
     4861 agcccggccc gggtcgcgta ctggaacccg agctccttga ggttgtcgag gatccgaccc
     4921 atttcgctcc gcgggtaggt ccgcacgcac tcgtcgacga tcctgctgac ctcgcccttc
     4981 aggacgtggt cgttcacgta tgggtagtcg agcgggaggg cctcgttgaa caacaccagg
     5041 ccggccgtcg tctcgatccg cgggaccccg ccgttctcct cgacgccggg cacgttcttc
     5101 ccgggcaggc gcacccggac ccagtggtgg accccgatcg tcccccggtc aagggccatc
     5161 agcacctcgg ccacgcccgt gaagctcggg atctcctcgg gcttctcggg cttgtcgtcg
     5221 gacgcggtca ggtagtagat cccgagcacc atgtcctggg tcggcgaggc gatcgtgcgc
     5281 ccgtgcgccg gggacaggat gttgttggtg gacagcatca ggatccgcgc ctccgactgc
     5341 gcctccgagg agaggggcag gtgcaccgcc atctggtcgc cgtcgaagtc ggcgttgaag
     5401 gcggggcaga cgagcgggtg gatctggatg gccttcccct caaccaggac gggctcgaac
     5461 gcctggatcc ccaggcggtg cagggtcggg gcccggttca gcatcaccgg gtggtccttg
     5521 atcacgtcct ccaggacgtc ccagacctgg ggtctggccc gctccaccat ccgcttggcg
     5581 ctcttgatgt tctgcgccag gccgcggtcc acgagctgct tcatcacgaa cggcttgaag
     5641 agctccaggg ccatctgctt ggggagcccg cactggtgca gcttgaggtt ggggccgacc
     5701 acgatcaccg agcggcccga gtagtccacg cgcttcccca gcaggttctg gcggaagcgc
     5761 ccctgcttgc ccttgagcat gtcgctgagc gacttcaggg gccggttgcc cgggccggtg
     5821 accggccgcc cacggcgccc gttgtcgaac agggcgtcca cggcctcctg cagcatccgc
     5881 ttctcgttgt tgacgatgat ctcgggggcc tggaggtcga gcagcctctt cagacggttg
     5941 ttccggttga tgacacgccg atagagatcg ttgagatcgc tcgtggcgaa ccggccgccg
     6001 tcgagctgca ccatcgggcg cagatcgggc gggatcaccg ggatcgcgtc gagcaccatg
     6061 cccatcgggg agttcttcgt cccgatgaag ctggagacca ctttcagccg cttgatcgcc
     6121 ttctggcgcc gggtgccctt cgcggtcttg atcgtctccc ggaggtcctc cgcctcctgg
     6181 tcgaggtcga gccccgcgag gagctcgcgg atggcctcgg cgcccatgcc gccctcgaag
     6241 tagtccccga agcggtcccg cagggtccgg tacacggagt cgtcggccac gagctgcttc
     6301 acgtggaggc tcttgaaggc gctgaggacc tcctcgaggt gctcggtctc gcgccgagcg
     6361 ccctcggtga tcttgtcgat ctcccgctgg gcctccttgt tggccgcggt gagctgtgcc
     6421 ttcttcgcct tctcggcctc gagctccttt atccgggcct cgagctcctt caggcgagcc
     6481 tggatctgct cgtcgcggcg cccgtcgacg gccgccttct cctcggcgat cgacttctcg
     6541 atccccggga ggtccttgtg ccggcgatcc tcgtccacgc gcgtgatcac gtaggccgcg
     6601 aagtagatga tcttctccag atccttcggg gccaggtcga gaaggtaccc caggcgcgag
     6661 gggaccccct tgaagtacca gatgtgggtg accggcgcgg ccagctggat gtgccccatc
     6721 cgctcgcgcc gcaccttggc ccgcgtgatc tccacgccgc accgctcgca gatgatcccc
     6781 ttgaagcgga cgcgcttgta cttcccgcag tggcactccc agtcgcgggt gggaccgaag
     6841 atccgctcgc agaacagccc atccttctcg ggcttcaggg tgcggtagtt gatcgtctcc
     6901 ggcttcttga cctcgccgtt cgaccaggct cggatctgat cgcccgtggc gagaccgatc
     6961 ctcagctcgt cgaagtagtt cacgtcgagc acgcgctcag ctcctctcga ctcactcgaa
     7021 atccgccccc gccggctcac gccgcgagag gtcgataccc agctcctccg ctgcgcggaa
     7081 gacgtcgtcg tcggtctccc tcatctcgat ctcctggccc tctgcggaga gcacctcgac
     7141 gttcagcgcg agggactgca tctccttgat gaggaccttg aaggactcgg ggatccccgg
     7201 ctcggggatg ttctcgccct tcacgatggc ctcgtacacc ttcacccggc ccaggatgtc
     7261 gtcggacttg atcgtcagca gctcctggag ggcgtaggcc gcgccgtagg cctcgagggc
     7321 ccacacctcc atctcgccga accgctgacc gccgaactgc gccttcccgc ccagcggctg
     7381 ctgcgtgatc atcgagtacg ggcccgtgga ccgggcatgg atcttgtcgt ccaccaggtg
     7441 cagcagcttc aggatgtaga tgtagcccac cgtcaccggc tgctcgaacg gccgtcccgt
     7501 ccgcccgtcg tagagcacag ccttgccgtg ctcgtcgacg agccggtcgc cgtccttgtt
     7561 gggcttggcc gtggccaggg cctccttgat ctcgtcttcg cgagcgccgt cgaacacggg
     7621 ggacgagacg aacgccggac cctcgtgctc cctgccgttc gacctgaacc cctggtgggc
     7681 cgcccacccc aggtgggtct cgaggacctg ccccaggttc atccgcgaag ggacgcccag
     7741 ggggttgagg atgatgtcca ccggcgtccc gtcgggcagg aacggcatgt ccgcctcggg
     7801 gagcaccttg gcgatcacgc ccttgttccc gtggcgcccg gcgagcttgt ccccgtcgga
     7861 gatcttccgc ttctgggcca cgtatacccg cacgagctgg ttcacgccgg gggagagctc
     7921 gtcgccgtcc tcgcgggtga agacgcggac cccgatcacc ttcccggact cgccgtgagg
     7981 gaccttcagc gaggtgtcgc ggacctcccg cgccttctcg ccgaagatcg cgcggagcag
     8041 ccgctcctcg ggggtgagct cggtctcgcc cttcggcgtg accttgccca cgaggtagtc
     8101 gccggtggtg acctcggcgc ccacacgcac gatccctcgc tcgtcgaggt ccttcaggat
     8161 ctcctcggag acgttgggga tgtcgcgcgt gatctcctcg ggcccgagct tcgtgtcccg
     8221 ggcgtccact tcgtgctcct cgatgtggat cgaggtgagc acgtcctcct tcgccagacg
     8281 ctccgagatg acgatcgcgt cctcgtagtt gtggccctcc caggacatga acgcgaccag
     8341 gaggttcttg cccagcgcca tctcgccctg atcggtggag gggccgtcag cgatgatctg
     8401 tcccgccgcg acccggtcgt cctcggtcac cacgggccgc tggttgatgc aggttccctg
     8461 gttcgagcgg cggtacttct gcagcttgta cggccgcagg ttgccgtccc gctccttgac
     8521 cacgacctgg tcggcggaga cctcctcgac ggtcccggcc ttctcggcca tcacgacgtc
     8581 gccggcatcc atggccgcgc gcagctccac ccccgtgccg atcagcggtg cctccgcccg
     8641 cagcagcggc acggcctggc gctgcatgtt cgcgcccatg agggcgcggt tcgcgtcgtc
     8701 gtgctcgagg aacgggatca ttgccgcccc caccgagacg atctgcttcg gcgagacgtc
     8761 catgtagtcg acctccgagg agggcacgta gtcgacctcg ccgcccttcc tccggacgag
     8821 cacccggtcg ctcacgaacc gcccgttcct gtcgagctcg gcgttggcct gggcgatgac
     8881 gaagaggtcc tcctcgtcgg cggtcaggta gtcgatctgg tcgctgacct tccccttctc
     8941 gaccttccgg tacggcgtct ccacgaaccc gaagtcgttc atgcgaccga aggacgagag
     9001 cgagccgatg aggccgatgt tcggcccctc gggggtctca atcgggcaca tccgaccgta
     9061 gtgggagggg tgcacgtcac ggacctcgaa gccggcgcgc tcccgggaga gcccgccggg
     9121 gcccagcgcc gagaggcgcc gcttgtgggt cagccccgcc agggggttcg tctgatccag
     9181 gaactgggag agctgggagg tcccgaagaa ctccttgatc gagcccacga ccggacggat
     9241 gttgaccagg gtcgtcgggg tgatcgcctc gacgtcctgg gtcgtcatcc gctcccgcac
     9301 cacgcgctcc atccgggaga gcccgatccg gatctggttc tggatcagct ctcccaccga
     9361 gcgcagccgc cggttcccga agtgatcgat gtcgtcggtg tcgaacccct cctggcccgc
     9421 gtgcagccgc acgaggtagc ggatcgtctt caccatgtcc tcgtaggtga ggaccgactt
     9481 gaacggcgcc gccccccgct cgttgccgta gggatgggag aagaccctcc acctgggctg
     9541 ctcgaactcc ttccgctcgg gcttgtcctg ctcccgcagc gtctcgtagc gtcgcttcag
     9601 ctgggtcttc agaaggtcgt cgacctcgcc cagcttcttg tccaccttgt agcggcccac
     9661 cttggccagg tcgtagcgct tcgggttgaa gaagaggttc tccaggaggt tgcgggcgga
     9721 ctcggctgtg ggcggctcgc ccgggcgcag cttccggtag atgtcctcca gagcttcctc
     9781 gggcgtctcc agcccgtcct tctcgagggt cgcggcgatg gtcggggcgt tatcgaacag
     9841 ctccatgatc tcgtcgctcg accagcccag ggccttgagc aggacggtga cgttctggcg
     9901 acgcttccgg tcgatgcgga cgccgacgag ctccttcttg tccacctcga actcgagcca
     9961 ggccccccgc gcggggatga tcttggcgat gtagatgtcc ttgtccgcgg tcttgtcgag
    10021 ctgcttgtcg aagtagaccc cgggcgagcg cacgagctgg gacacgacga cccgctcggt
    10081 cccgttgatg atgaaggtcc cgcgctcggt catcatcggg aactcgccca tgaagaccgg
    10141 ggcggacttg atctcgcccg tctgcttgtt gatgaactcg gcctggacgt ggaggcccgc
    10201 cgagtacgtc atgtccttgt ccttgcactc gtccacggag tgcttgacgt cctcgaacca
    10261 gaagtccccg aaccggagct cgtaggtctc ggtgtagtcc tcgatcgggc tgatctcgtc
    10321 gaagacctct cggagcccct cggtgatgag ccagtcgaag gactcgcgct ggatcgcgat
    10381 caggtcgggc agctcctggg gctcctggag cttggcgaag gacacgcgct cacgaccggt
    10441 cacggcgttc ggcaaagaga tcccccccta gggaacgaca tgcgtgtaac tcgcgtacgc
    10501 gggagacggc acggcaaacc gctagtttag gcctccgcgc gcagagtgtc aacggggacg
    10561 cctcgaagca ggccacccct tgggcgaccc ggtgggcggg gtcggtgagc cctccaacct
    10621 actggagctc gacggtcccg ccggcggcct cgagcttctc cttggcctgc tcggccgtct
    10681 ccttgttgac cttctccagg accggcttcg gagcaccgtc cacgagctcc ttggcctcct
    10741 tgaggccgag gttcgtgaga ccgcggacct ccttgatgac ctggatcttc ttgtcgccgg
    10801 cggccgagag gatcacgtcg aactcgtcct tctcctcggc tgcggcgcct gcgtctccgc
    10861 ccggcgcccc ggccaccgcc acgggagccg ccatcgacgc ggcctccaca tcgaacgtct
    10921 cctcgaactg cttgatgaac tccgagagct caaggagcgt catccccttg aaggcctcga
    10981 ggaggtcatc gccggagagc ttggccatct acccttcctc ctgctcctcg tcctgcgctt
    11041 cctcggtctc ctcctccgcc tgctcggcgg aagcctgttc gtcgcccccg cccgcctcgt
    11101 cctcgctcgg agccttctcg gcgggggcct cctcctccag cttctccgcg taggcgctca
    11161 gcagcgaggc gaacctcgac tgcagggcct gcatcaggta cgcggccttc cgcatgtgca
    11221 tcgagaggag gccggccacc ttggccagca tctcctcccg ggtctcgagc gccgcgagcg
    11281 cccggatctc gtcggctccc aggacgcggc cctcggccag cccccccttc acctcgagca
    11341 ccgggaagcg cttcgtggcg tccacgaggg ccttcgcccc cgccacgatg tcgccctgga
    11401 cgaacgtcac cgccgtgggc ccctccagga aggactcaag ctcggacatc tcgaggtccc
    11461 ggaccgccag acgcgtcagc gtgttcttca ggaccgtgaa ggcggcctcg gcttcgagga
    11521 gggccgtacg gacctccccg atatcgggga cggagaggcc acggtagtcg gcgaagtagg
    11581 ccgcctgcgc ggagcgcagc cgctccctga tctcctcgac cttctcgacc ttctgctggt
    11641 tcggcatcgt gctcctcaca agggaaaagc gcctccgcag cacagcccgg gaaggcgctt
    11701 cactgcctcg gcaggtgccc cctcgggatt acgcggttgt cggcccgccc ctgctgtctg
    11761 cggcgacccc aaggatacac acggcgaccc ggaccgacaa cgcctcaggc cggcaccgac
    11821 tcggtcacgt ccttgatctt ggacgggtcg atgcggatcc ccgggcccat cgtcgaggac
    11881 aacgtgaccg agcggatgta gcggcccttg gcgccggccg gcttggcccg gagcacctcg
    11941 tccaccaccg tcaggtagtt ctcggacagg gcctggagct cgaacgagcg cttcccgatg
    12001 atgagatgga ggttcccggc cttgtccacg cggtactcga gcttcccgcc cttgatgtcc
    12061 tggaccgcct tgccgatgtc ctcggtcacc gtgccggcct tggggttcgg catcaggccg
    12121 cgggggccga ggatgcggcc ggccttcccc acgatcggca tcatgtcggg ggtggccacc
    12181 gccgcctcga actcgatcgt gccgtcgagg accgtctcca gcaggtcctc gccgcccaca
    12241 acgtcggcgc cggcctcctg cgcctcgcgg gccttgtcgc ccacggcgaa cgcggccacg
    12301 cgcacgctct ggcccgttcc gtgcgggagg gagacggtgc cgcgcaccat ctcttccgcc
    12361 ttccgcgggt ccacaccgag ccggaacgag agctcgacgg tctcgtcgaa cttcgccttc
    12421 ggcagctcgt gcaggatccg cagggcgtcg gccggcgtgt agagctgctc gcggttgatg
    12481 gacctcatcg cgtccgtgta tcgcttgccg cccatcagcc caccacctcg atccccatcg
    12541 agcgcgccgt cccctcgatg atccgcatcg cgttctcgag gtcgcgcgcg ttgaggtcct
    12601 tcatcttcgt ctccgcgatc tggcggacct ggctcctgga gacctgaccc accttctcgc
    12661 ggttgggctc gcccgagccc ttctccaccc gcgccgcctg cttcagcagc tcggccgcgg
    12721 gaggctgctt cgtgacgaac gtgaacgagc ggtcctcgta gatcgtcagc tcgacgggga
    12781 tcatctggcc catctgggcc tgcgtggcct cgttgtaggc cttgcagaac tccatgatgt
    12841 tgacgccgtg ctggcccagg gccgtgccca ccgggggcgc cggcgtggcc tgccccgcgc
    12901 ggatctggag cttgaccacc gccaggacct tcttgccggg tcgctgtgcc atgtgcttcc
    12961 tcctcgtggt ccgtgcggca ccctcggggg cgcctcccac cgatcagacg cgggccacct
    13021 ggtcgaagct gagctcgacg ggtgtctcgc gatcgaagat gttcaccatc accttgacct
    13081 tggactggtc cacgttgatc tcgctgatgg tgccgttgaa gtccgcgaat ggccccgcga
    13141 tgatccgcac cacatcgccc tcctcgaact ccagccggaa ctggggcttc gcctcctcct
    13201 tctgaggggc gaggaacttc tccacctcgt gccgaggcag cggggtgggc ttctggcccg
    13261 tccccgcggc cacgaacccc gtcaccccgg gggtgttccg gacgacgtac caggagtcgt
    13321 cgtcgtactc catccgcacg aggagatagc ccgggaacac cttcttctgg acgacctgct
    13381 tgcggccacc cttgatctcc atcacgtcct ccatcgggat gtagacggag aagatcttgt
    13441 cctgcatctg catcgtctgg atgcgcgact cgaggttcgt cttgaccttg ttctcgtagc
    13501 ccgcgtacgt gtggatcacg taccagtcgc cggggccgga gaacgcatcg ctgggcgcct
    13561 cggccgcggg ctcgggcgcg gccacctcct ggtcgggagc gggggcctcc tcggtgggat
    13621 gctcggcggg ctcggcgggc tcctcgggct cctccaccgt ctgcaccgca gcctcctcgg
    13681 ccgcctgcgc ggcgtcctcg tccgtggcct cggcggcctg ctcggccatc tccgccacca
    13741 cggcctcgcg ctgttgttcc gtagcctcct cgaccccggt gaggctcgcc tcctgggcct
    13801 gctcggaggg gaggacgtcc tgctcttcgg gcatccctcc ctcgcccgcg ggaggagcct
    13861 catcctcgcc tcgggtgggc atcgcctcgt cggtcagctc gcgctcagcg gcctcggagg
    13921 cctcccggtc ggaggcatcc tgggtctgct cgtcgttctc ggggaactcg tccatgggct
    13981 cgcggctact cgatgccgaa gacggtgaag atgcccttgc cgatcacaaa gtcgagcgcg
    14041 aacacgaagc tcgtcaggac gaccacggtc accaggacga cggtcgtgta gatcagcatc
    14101 tgcctgcgat ccggccagtt caccttattc agctcctggc gaacctcgcg caggaactgc
    14161 cggaagccgg tccgctcgcg cttcttctgc tcgaccgcct ggcgccggcg cgccggagcg
    14221 gacgggcgtt caccgcccgc acgctgctgg cgctgcatgg ctcgcttcgc ttgtctgttc
    14281 atccctgctt ccttactcgg cagggccgga gggactcgaa cccccaaccg ccggttttgg
    14341 agaccggtgc tctgccagat tgagctacgg ccctcggcac c
//
LOCUS       DASUTZ010000240         2731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1254343, whole genome shotgun sequence.
ACCESSION   DASUTZ010000240 DASUTZ010000000
VERSION     DASUTZ010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254343"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..95
                     /locus_tag="VF097_10285"
     tRNA            22..95
                     /locus_tag="VF097_10285"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56..58,aa:Pro,seq:tgg)
     gene            160..531
                     /locus_tag="VF097_10290"
     CDS             160..531
                     /locus_tag="VF097_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015662618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6263221.1"
                     /translation="MLGDIEAFSGFAVADLEKAQEFYGDTLGLKVSMLEEDQLMTLHI
                     AGGRDVFVYAKPDHTPATYTILNFPIDDIDAAVDGLAARGVVFERYEGFEQDEKGVAR
                     GQPDIAWFKDPAGNILSVLHG"
     gene            complement(781..1350)
                     /locus_tag="VF097_10295"
     CDS             complement(781..1350)
                     /locus_tag="VF097_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263222.1"
                     /translation="MDRGRMRPVVGLLVATVALGVFTLAPVSAHFTQDTRHLGKHAWQ
                     QVIKKKTTELSARAYGAAAINGVFFSQPTFANILRRPVTAPRKGYLHIVAHLMANDDS
                     SMTGPGLLHYRLTVDGTTVGTEYFMAYGGSPGTIQIGGATAVVKVGKGDHMVALQGKE
                     VGSGSIIDDRDLSVLFVPFGSTTLPVAAP"
     gene            complement(1409..1945)
                     /locus_tag="VF097_10300"
     CDS             complement(1409..1945)
                     /locus_tag="VF097_10300"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX6263223.1"
                     /translation="MLKGPTLRLRTVRESDLGELHADLSDLEHRGAFFPLGLQSEPAF
                     RRQFEENGFWGQDEGMLLMVDPHDRVVGEIEFYPITHYLTGYELSYLVFGSEHSGKGY
                     ATEAVRLMTAYLFARQRIDRVQLAIHPENEASRRVAQKAGYTFESLMRRCWFHQGRFH
                     DLEIWSMIRDELPPEAGT"
     gene            2051..>2731
                     /locus_tag="VF097_10305"
     CDS             2051..>2731
                     /locus_tag="VF097_10305"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HEX6263224.1"
                     /translation="MDERKLPLFDMIGSDRPRGDEHWLGRALGILLIPIVVLIASMLV
                     AGALAPVVGASAMLVGTAHDLLGPEPSEEELAFPRLPQRSTIYAADGSVLAKLFLNEN
                     RKIVRLRRLKPMTVRAVIAAEDEHFYEHNGVDFGAVARAGLRNLATGRISEGASTITQ
                     QVARSLFEEVGTEDTLRRKVREARVAVKLERVYTKDEILEIYVNEVYLGHGVYGIATA
                     AEWYFAKPV"
BASE COUNT          436 a          924 c          936 g          435 t
ORIGIN      
        1 cgggccgtag aatcgagcga gcggggcgtg gcgcagcttg gcagcgcgcg tgctttggga
       61 gcacgaggtc ggcggttcga atccgcccgc cccgaccggc gatgaggagc cgggctccgg
      121 atcgtcacta tggacgaagg cgatcgagag gagctcagca tgttgggaga catcgaggcg
      181 ttcagcggct tcgccgtggc tgatctggag aaggcgcagg agttctacgg cgacacgctc
      241 gggctgaagg tgtcgatgct ggaggaggat cagctgatga cgctgcacat cgcgggcggt
      301 cgggacgtct tcgtgtacgc gaagcccgac cacacgccgg ccacctacac gatcctcaac
      361 ttccccatcg acgacatcga cgccgcggtg gacgggctgg ccgcgcgcgg cgtcgtcttc
      421 gagcgctacg aaggcttcga gcaggacgag aagggcgtcg cgcgcggcca gcccgacatc
      481 gcgtggttca aggacccggc gggcaacatc ctctccgtgc tccacggcta acagggcctt
      541 cccggccggg ccggttcctc gcgcgcttcc ggtcgaaaag gtttcatcat ggggccacga
      601 tgcgaaggtg gctcgccccg ctcgtgatcg tggcgacggt gaaatgcacg cagacgaaca
      661 ccgtccccgt ggtcaggtcg gcggacgaag ggagcgcccg cccggaggcc ggggccccga
      721 gccgggctgg cagtggctcc ccgcatcgcc cctcagccct cgccagaacg ccaacgcggc
      781 ctacggcgcg gcgaccggga gggtggtgga gccgaagggc acgaagagca cgctgagatc
      841 gcggtcgtcg atgatgctgc ccgatccgac ctccttcccc tggagcgcca ccatgtggtc
      901 gcccttgccc accttcacga ccgcggtcgc ccctccgatc tggatggtcc ccggcgaccc
      961 gccgtacgcc atgaagtact cggtccccac ggtcgtcccg tcaaccgtca atcgatagtg
     1021 aagcagcccc gggcccgtca tcgagctgtc gtcgttggcc atcagatgcg ccacgatgtg
     1081 gaggtagccc ttgcggggag ccgtgaccgg ccgtcggagg atgttcgcga aggtcggctg
     1141 cgagaagaac accccgttga tcgcggcggc tccgtaggcg cgcgccgaga gctcggtggt
     1201 cttcttcttg atcacctgct gccatgcgtg cttccccagg tgtcgcgtgt cctgggtgaa
     1261 gtgagccgag accggggcga gcgtgaacac cccaagggcc acggtcgcca cgagcaggcc
     1321 gacgaccggc cgcatccgtc cgcgatccat gctggacctc ctggtcgtgt gacccgtcga
     1381 gtatggccgc ggggtctgtg ggaagccttc aggttccagc ctcgggtgga agctcgtccc
     1441 ggatcatcga ccagatctcg aggtcgtgaa accggccctg gtggaaccag cagcgacgca
     1501 tgagactctc gaacgtgtag ccggccttct gcgccacccg ccgcgacgcc tcgttctctg
     1561 gatggatggc cagctgaacg cgatcgatcc tctgacgggc gaacaggtac gcggtcatga
     1621 gcctcacggc ctcggtggcg tagcccttgc cgctgtgctc cgagccgaac acgaggtagg
     1681 agagctcgta gccggtcagg tagtgggtga tggggtagaa ctcgatctcc ccgaccaccc
     1741 ggtcgtgggg atcgaccatc aggagcatcc cctcgtcctg gccccagaag ccgttctcct
     1801 cgaactgccg tcggaaggcg ggctcggact gcagtcccag cgggaagaac gcgcctctgt
     1861 gctcgaggtc gctcaggtcg gcgtggagct ccccgagatc gctctcgcgg acggtccgaa
     1921 ggcggagcgt cgggcccttg agcatcccgg cgatgctagc gcagggcgtc gcccggcgcg
     1981 cttgctctcg tgtccctctc ccggcggggg ggaatggtga tagcaggcct tcgacagatc
     2041 caccgaggag atggacgagc gcaagcttcc tctgttcgac atgatcggct cggaccgccc
     2101 gcgcggcgac gagcactggc tggggcgcgc gctcggcatc ctcctcatcc ccatcgtcgt
     2161 cctgatcgcc tcgatgctcg tcgcgggagc cctcgctccc gtggtcggcg cctccgcgat
     2221 gctcgtgggg actgcccacg atctcttggg ccccgagccc agcgaggagg agctggcctt
     2281 cccccgcctg cctcagcgct ccacgatcta cgccgccgac ggctcggtcc tggccaagct
     2341 cttcctcaac gagaaccgga agatcgtgcg cctccgccgc ctgaagccca tgaccgtccg
     2401 ggccgtgatc gcggccgagg acgagcactt ctacgagcac aacggcgtgg acttcggggc
     2461 ggtggcccga gccgggctgc gcaacctggc caccggccgg atctccgagg gcgcgtccac
     2521 gatcacccag caggtcgcca ggagcctctt cgaggaggtg gggaccgagg acaccctccg
     2581 tcgcaaggtc cgcgaggccc gtgtggccgt gaagctggag cgcgtctaca cgaaggacga
     2641 gatcctcgag atctacgtca acgaggtcta cctgggccac ggcgtctacg ggatcgccac
     2701 cgcggccgag tggtacttcg ccaagcccgt g
//
LOCUS       DASUTZ010000241         2104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_995514, whole genome shotgun sequence.
ACCESSION   DASUTZ010000241 DASUTZ010000000
VERSION     DASUTZ010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995514"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1375
                     /gene="sucB"
                     /locus_tag="VF097_10310"
     CDS             <1..1375
                     /gene="sucB"
                     /locus_tag="VF097_10310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HEX6263225.1"
                     /translation="QLGETVVEGTIVKWLKSEGDSVDRDEPLFEISTDKVDTEVPSPI
                     AGTITEILVQEGETVEVGTQLAMIESGDGQAAAEAEPSEEAEEPKEEAQAREEKAEEP
                     PQPQPESAPQSEQPAQPEQPAPAQPEQQQAQPAAAAARGPRSHILSPLVRRLADEHGI
                     DLDRVQGSGTGGRITKKDIMAVVESGQPAAEAAAAEARPPLQAVPEEPRPAPAPQAAP
                     AAAEEVRPISHIRKRIAENMVASLHQSARAWNAVEVNMERIAQLRNRAKDAFKREEGI
                     SLTYMPFLARAVSDALLAYPDVNARLNLEEGTATYRRSVNLGVAVAYDEGLIVPIVKG
                     AEGMNVVGLARAINDVATRARSKKLTPDEVHGGTFSITNPGPFGSLISVPIMNPGETA
                     ILAFDTVQKRPVVIDDAIAIRHMVYLSLSWDHRMIDGATAAQFLARIKENLETWDFTS
                     EVQAYLT"
     gene            1372..2058
                     /gene="lipB"
                     /locus_tag="VF097_10315"
     CDS             1372..2058
                     /gene="lipB"
                     /locus_tag="VF097_10315"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX6263226.1"
                     /translation="MTGRAWLLAPGRVPYSSALEAMRELVDRRIVGEVPDTLLLLEHE
                     PVYTAGRRTDPSHLLMAERDLRARGAELHHVDRGGSVTFHGPGQLVGYPIVHLGVAPS
                     LAQHVRRMQDVVIRSCADVGVRAAPDDEVGVWCGDRKVCAIGVKSTRRVTYHGFALNC
                     TTDLSWFDAIVPCGLADRGVTTLSQVAGRTVTVEEMTSIVARHFSEVFDRELSPAPAE
                     AAPALGRTVA"
BASE COUNT          323 a          730 c          788 g          263 t
ORIGIN      
        1 gcagctgggc gagaccgtcg tcgaggggac gatcgtcaag tggctgaaga gcgaggggga
       61 cagcgtcgac cgcgatgagc ccctgttcga gatctcgacc gacaaggtgg acaccgaggt
      121 gccctcgccc atcgccggca cgatcaccga gatcctggtg caggagggtg agaccgtcga
      181 ggtcgggacc cagctggcga tgatcgagtc gggggacggc caggcggcgg ccgaggccga
      241 gccctccgag gaggcggagg agccgaagga ggaggcccag gcacgggagg agaaggccga
      301 ggagccgcct cagccccagc ccgagtcggc tcctcagtcg gagcagccgg cccagccgga
      361 gcagccggcg ccggcccagc cggagcagca gcaggcccag ccggccgccg cggccgcgcg
      421 aggaccccgg agccacatcc tctcccccct cgtccggcgg ctcgccgacg agcacgggat
      481 cgacctggac cgggtccagg gcagcggcac gggagggcgg atcacgaaga aggacatcat
      541 ggcggtcgtc gagtcggggc agcccgccgc ggaggcggcc gcggcggagg ctcggccccc
      601 gctccaggcc gtccccgagg agccgcgacc ggctccggcc ccccaggcgg cgcccgcggc
      661 cgccgaggag gtccggccga tctcgcacat ccgcaagcgg atcgccgaga acatggtggc
      721 gagtctccac cagtccgcgc gggcctggaa cgccgtcgag gtcaacatgg agcggatcgc
      781 gcagctccgg aaccgggcca aggacgcgtt caagcgggag gagggcatct cgctcaccta
      841 catgccgttc ctggcccggg cggtgagcga cgcgctgctg gcctaccccg acgtcaacgc
      901 gcggctgaac ctcgaggagg gcaccgccac ctaccggcgg tcggtgaacc tcggggtggc
      961 cgtggcctac gacgaggggc tcatcgtccc tatcgtgaag ggggccgagg ggatgaacgt
     1021 ggtggggctg gcgcgcgcga tcaacgacgt ggccacgcgg gcccgctcca agaagctcac
     1081 gcccgacgag gtgcacggcg ggaccttctc cataaccaac ccagggccgt tcggctcgct
     1141 gatctcggtc ccgatcatga acccgggcga gacggcgatc ctggcgttcg acacggtgca
     1201 gaagcgccct gtcgtgatcg acgacgcgat cgccatccgg cacatggtct acctctcgct
     1261 gtcctgggac catcggatga tcgacggggc cacggctgcg cagttcctgg ctcggatcaa
     1321 ggagaacctc gagacctggg acttcacgag cgaggtccag gcctacctga cgtgacggga
     1381 cgggcctggc tgctggcgcc ggggagggtt ccctacagct ccgccctcga ggcgatgcgg
     1441 gagctcgtgg atcgccgcat cgtcggggag gtccccgaca cgctgctgct gctggagcac
     1501 gagcccgtct acaccgcggg gcggcggacc gacccctcgc acctgctcat ggcggagcgc
     1561 gacctgcggg cccggggggc ggagctccac cacgtggacc gcggcggcag cgtgacgttc
     1621 cacgggccgg gtcagctcgt gggctacccg atcgtccacc tcggcgtggc gccgagcctg
     1681 gcgcagcacg tgcggcggat gcaggatgtc gtgatccggt cgtgcgccga cgtcggggtg
     1741 cgcgccgccc ccgacgacga ggtcggcgtc tggtgcggcg atcgcaaggt gtgcgcgatc
     1801 ggggtgaaga gcacgaggcg cgtcacctat cacggcttcg cgctcaactg cacgaccgac
     1861 ctctcgtggt tcgatgccat cgtgccgtgc gggctggcgg accggggcgt gacgacgctg
     1921 tcccaggtgg cgggccgcac cgtgaccgtc gaggagatga cctccatcgt ggcgcgccac
     1981 ttctccgagg tcttcgatcg cgagctgtcc cccgcgcccg cggaggccgc cccggcactc
     2041 ggccggaccg tggcctgagc ggcctcagcg cctctcgccg tgcggctggg gctcactccg
     2101 ggga
//
LOCUS       DASUTZ010000242        22343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_348488, whole genome shotgun sequence.
ACCESSION   DASUTZ010000242 DASUTZ010000000
VERSION     DASUTZ010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 22343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22343
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348488"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VF097_10320"
     CDS             complement(<1..180)
                     /locus_tag="VF097_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEX6263227.1"
                     /translation="MFLRENEIGRRIAPWVEPGMRLLDVGSGTGRIARWLTRERRVLP
                     TTSDLHEFDNRVGDLP"
     gene            260..1063
                     /locus_tag="VF097_10325"
     CDS             260..1063
                     /locus_tag="VF097_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263228.1"
                     /translation="MSERCPRCGGLNAPDAEWCGQCFTRLGEPEEPAIDPAVDPVEAA
                     AMLGELASPEERTAEPAPGVPSDQAGGEPAGAPPGGPPADEQPAEGEPPLQPGPATEA
                     SPFQVREDGLVWVCRLCEAENPLEAHVCARCGTPFGALFEEGREASEIDPGRASALSL
                     IFPGLGHLAAGHRVEGLARILIFVWLIGTIVVIVAARAGRGLGPLLGLVVLYMASAGA
                     LYMLSAIDARRATEGDDPIVSSRILLYGISALLLVTIVFLFFSGPTLRG"
     gene            1098..1535
                     /locus_tag="VF097_10330"
     CDS             1098..1535
                     /locus_tag="VF097_10330"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6263229.1"
                     /translation="MADRTEGDIQIEAEPGDVMEVLVDFDAYPEWAGVESAEVLEEDD
                     EGQPAAVAFRLSAMGFDATYTLEYAFAPDDGGMSWTTREAAGALKDVAGEYVLEASDD
                     GTHVTYRLAVELAVAVPSLLRKQGEKRIVTTALEGLKRRVEEG"
     gene            1582..2778
                     /locus_tag="VF097_10335"
     CDS             1582..2778
                     /locus_tag="VF097_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HEX6263230.1"
                     /translation="MRILLFTGKGGVGKTTIAAATAVRAARAGRRTLVTSTDPAHSLA
                     DAFDVQIGDAPLQVEDRLWAEQVNAQARLESNWRNIQEYIIQFLNWAGVDAIEAEELS
                     VIPGLDEIFSLTDMKRHVDVADYDLLVIDCAPTAETLRLLSLPEVMNWYIERIFPVER
                     RVVKTIRPILTRLTTMPIAEDRIFAAIEQLHRNLEGVRAILGDAERSSVRLVVNPEKM
                     VIAEARRTYTYLSLFGYRVDAIVANRIIPEEVNDPYFGKWKDIQAEHLATIHESFQPI
                     PILTSRLFGQEMVGPDLLEEMGDEVYGEGDPSAVLYSEEPMRIRRRGPLYLLTLRLPF
                     VDRGALDIHRKGEELFVRVGSHKRNLILPQALQRLDVRDAQLVDDRLEIAFGEEAQSR
                     AGAGGG"
     gene            2796..3026
                     /locus_tag="VF097_10340"
     CDS             2796..3026
                     /locus_tag="VF097_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263231.1"
                     /translation="MAEKETAREEGTDVPTCPVSICPVGMMLTLAGQAKPEVVEHLLN
                     AGRELIMAATVFLNARADAVSESPRLERIDVD"
     gene            3043..4164
                     /locus_tag="VF097_10345"
     CDS             3043..4164
                     /locus_tag="VF097_10345"
                     /inference="COORDINATES: protein motif:HMM:NF037429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vms1/Ankzf1 family peptidyl-tRNA hydrolase"
                     /protein_id="HEX6263232.1"
                     /translation="MATLDQGLLRKLSDWSPAHPVTSLYLDVDGRRHPSRNGYLVRMR
                     DLFKGMSLDGLDREQRRSVAEDMEAIRRHVEDELDRSRVGGVAVFSSARSGLWESVPV
                     SRPFRDRLHVGSRPLLIPLEGMLERFETFCMLVADREHARVLYVRQDLVEELGEIRDD
                     VPGRHEQGGWSQARFQRHIEDHVLRHLKHTAQVALRASQRQPFDHLILAGSEELVADL
                     ERELHDYVARKVLDRIVLPITVSADEVRERVAAIEAELESRREAEALERLTIEARAGT
                     GRAVAGLAGTLEALESGRVETLVVAADLRADGRRCARCGHLSLAEARCAACGADTEAA
                     DHIVEEAVELAVRQKCRVETIGAAEELGPLGGVGALLRF"
     gene            4168..5121
                     /locus_tag="VF097_10350"
     CDS             4168..5121
                     /locus_tag="VF097_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX6263233.1"
                     /translation="MGERPLAIGLDVGGSKVSGVLMSDEGSVLASELLASPADDVEAM
                     VETVVLAAERLRAEGPAAAVGLGAAGMVEHDTGVLRSAPNIAWTEVPLRDLVRRRIGL
                     PCVVDNDANTAAWGEFLFGAGRGLRHVLLVTVGTGIGGGVIQDGRLYRGAHGFAAEIG
                     HIIVEPDGPRCGCGNRGCWEQMASGQALERTAREVALDDPEGPIARAAGGRPPVGPDV
                     VTAAAGGDPAAGTILETVGRRLGEGLAGLANVLDPEAIVVGGGMAEIGAPFLEPARAR
                     FLETVEAPDHRPEIPILQAVLGNDAGAIGAAAMALEFLEAE"
     gene            5118..5993
                     /locus_tag="VF097_10355"
     CDS             5118..5993
                     /locus_tag="VF097_10355"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6263234.1"
                     /translation="MRIGVVLPLFSGDPDKVLAAARETEALGYDGAFVFDHFFPPGAA
                     PDRPSLEAFTTLAAVAAVTERIRVGTLVTRASLRPPGILAKQASWIDAISGGRLILGI
                     GTGDETDADEHRAFGLPLMGRGERRAHLLETVRALRSMFEGEGYSGGERVPPLSGPLV
                     PAAAPPIWVAGLADPVVRLAAREADGWNGWGLDAPAFARKAALLRAEAGDRTVEATWA
                     GIVLVGRDDAELAELKARRADRGMEDASWSGTAVELAGFLTQLGREGAGWGILVAAGP
                     RDRRELIAREVLPQL"
     gene            6049..7374
                     /locus_tag="VF097_10360"
     CDS             6049..7374
                     /locus_tag="VF097_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX6263235.1"
                     /translation="MAKRVVIMGAAGRDFHNFNVVYRGHPEVEVVAFTATQIPGIENR
                     RYPPELAGNRYAEGIPIRMEDELADLIANERVDEVVFAYSDVSHEYVMHRASAALAAG
                     ASFTLLGPEATMLDSTKPVVAVCAVRTGSGKSQTTRAVVRALKTHGRKPGVVRHPMPY
                     GDIVAQRVQRFATMDDLDAADVTVEEREEYEPHIASDTVVYAGVDYAEILEAVQQEAD
                     VVIWDGGNNDLPFYRPDVHLVVADPHRPGHETRYHPGEANLRMGDVIVINKVDTAEPE
                     NVEAVEATARELNPDAVIIRANSPVTVDDPDAVEGARVLVIEDGPTLSHGEMTYGAGV
                     VAARQHGAAEIVDPREWAAGSLKSVYEKYPVGPVLPAMGYSDEQIRELEDTIDAVPCD
                     LVMVATPVDLSRIASFSKPSVRVGYELEEAEGQPTIAEVLAARLPAAGG"
     gene            7376..8308
                     /gene="arcC"
                     /locus_tag="VF097_10365"
     CDS             7376..8308
                     /gene="arcC"
                     /locus_tag="VF097_10365"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014789044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEX6263236.1"
                     /translation="MPRVVIALGGNALLQREEEDTAEVMVRNARTAAEQVADVAASGW
                     EILVTHGNGPQVGRILLQNEAARDWAHPMPLDVCGAMSQGQIGYLLQVTIGDVFYERG
                     MERPVTTLLTLTRVRADDPAFQDPTKPIGPFYEGAEAERLAEERGWVVKEVGEGGWRR
                     VVPSPRPYSIVEAPIAKQLAESETIVICTGGGGVPVIEEGPRLVGVEAVVDKDHASAI
                     MAREVEAQVLMILTDVRGLYERFGEPDQRLLERLTVDEAERVLDSLPAGSMGPKLEAC
                     VAFVRGGGERAVIGPLDGALAALEGRIGTSIVSA"
     gene            8331..9002
                     /locus_tag="VF097_10370"
     CDS             8331..9002
                     /locus_tag="VF097_10370"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX6263237.1"
                     /translation="MELRVVVWNAHGLRAGVAAAAETLARHEPDLVVLNEVGWSGWRL
                     ARLARALDARAASGLRRLRWGVTNAVLARPPWRLLRTRTIRLPGRSRPHRGVVAALVG
                     RSGYRLTAASVHLGLSDPERREHARGLTDRILPELRAPVLLGGDLNEAPEGPAARWMT
                     ERLWDAFAQAGRGRGETFPARDPRARIDYLLVPEGVTVAQAFVDRDAAEASDHLPLVA
                     DLELP"
     gene            complement(8999..9748)
                     /locus_tag="VF097_10375"
     CDS             complement(8999..9748)
                     /locus_tag="VF097_10375"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6263238.1"
                     /translation="MSTEIVPGAEPFFFPGGDVAVLMLHGFTGNPSSIRPWGEALAER
                     GFTVSGPRLPGHGTTWQDLATRRGDEWVAEADAALTRLRGDGGRVVICAQSFGGTIAL
                     DLASRRPGDVRGIVLVNPYVRDPRHVWLPVVRPFLRTIKGIGNDIKKPGGNELPYPRV
                     PLRAVSEVARMMKRLETVIPSIVQPLLLFHSPQDHVVPRGTAEWLLDRIGSEDKELVL
                     LPDSYHVAALDNDAPTIFERSAAFIERVTRG"
     gene            complement(9745..10113)
                     /locus_tag="VF097_10380"
     CDS             complement(9745..10113)
                     /locus_tag="VF097_10380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6263239.1"
                     /translation="MTLRKTILLVEDDDSVRQLVRVTLELHDFDVVEASGAAEGLELL
                     ERHRPDAIVLDLMMPGMGGERLLTQLRAAPATRDTPVVVVSGKPEVGHEVLGLVGREN
                     FIVKPFDPQALIDRVRAAVG"
     gene            complement(10118..10267)
                     /locus_tag="VF097_10385"
     CDS             complement(10118..10267)
                     /locus_tag="VF097_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263240.1"
                     /translation="MESGWGVLEWTFVIVVGSLSLAAGLFALYLLALPFRNTGLRRRP
                     NPRGR"
     gene            10342..11079
                     /locus_tag="VF097_10390"
     CDS             10342..11079
                     /locus_tag="VF097_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX6263241.1"
                     /translation="MFYWFLKHVLLGPLLRLIYRPKAVGLEHVPAEGPVILASNHLSF
                     VDSLFIPLLVKRPVVFLGKADYFDSWRTRWFMKAVNVIPVRRTGGSASEAAIRTGVEA
                     LEKNLVIGIYPEGTRSPDGRLYRGKTGVARMALEAGVPVVPIAVDGTVDIMPLDKKMP
                     RLRGRVTVRFGAPLDFSRYYDRPRDRFVLRSVTDEIMYEIMMLSGQEYVDEYAAKVKK
                     ELAAQEERTVTVPDTETVDQDSRSGTA"
     gene            complement(11080..12573)
                     /gene="glpK"
                     /locus_tag="VF097_10395"
     CDS             complement(11080..12573)
                     /gene="glpK"
                     /locus_tag="VF097_10395"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEX6263242.1"
                     /translation="MSPVARILAIDAGTTGVKALVVNEAGSVLGTGYREFAQSFPRPG
                     WVEHEPEDWWTATQEATLAALRAADLRTSDLTALGITNQRETTVLWDRETLHPVHPAI
                     VWQDRRTAPLCAELVDEGWEERVIERTGLVIDPYFSGTKLAWLLDQVEGAREAAEAGR
                     LAFGTVDAYLISRLTGGRAHVTDHTNASRTMLFDILRLDWDPELLERLRIPREVLPEV
                     GPSSGSFGETDPDAFLGIHVPIAGVAGDQQAALVGQACFTPGQAKNTYGTGSFVLLNT
                     GDHAPRAKGLLTTIAYSDEEGVRYALEGSIFVTGAAIQWLRDGLGIIETSADIGPLAA
                     SVPDTEGVYFVPALTGLGAPWWDPHARGTIVGITRGTNRAHLARAAVESMAFQTRDVV
                     DRMVADSEVGLSQLKVDGGASVMDLLCRFQADLLGVPVRRPKVQETTSLGAAYLAGLA
                     TGVWSSKDEAARAWELDAEFLPGDEDLTARYDDWRRAVERSLAWARG"
     gene            complement(12598..13620)
                     /locus_tag="VF097_10400"
     CDS             complement(12598..13620)
                     /locus_tag="VF097_10400"
                     /inference="COORDINATES: protein motif:HMM:NF015298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlpA-like double-psi beta-barrel
                     domain-containing protein"
                     /protein_id="HEX6263243.1"
                     /translation="MPRFLAAVVATLLAFLGGTSAAADPLDDARAERDAVRSEVASLR
                     GRLATLGRRYDALDERAGRLAAALVDDYRESLRLDADLDHARNTLSDRARAAYQMGPA
                     GFLEAFLSAGSVTDMLVVREAIQRTFVDDVERVAGVLEERGARAMVERRLEQRRRELF
                     SLQRRLEGLRQEMGFALLRAREEARRAGARVAALKREARRLAAAERRARARAPLITGG
                     LDQSELLALLGPDGGRGCDIPSGLRPTGSGFEGIASWYGWDFAGRPTASGAIFDPRLF
                     TAAHRTLPLPSFLHVRHGGRCATVLVNDRGPFIEGRVLDLSLGAAEYLGVGLSHVEAE
                     ILVPRG"
     gene            13683..14714
                     /locus_tag="VF097_10405"
     CDS             13683..14714
                     /locus_tag="VF097_10405"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEX6263244.1"
                     /translation="MATIGILNGGGDCPGLNAVIRAVVRKGVEDYGDRILGFRDGYRG
                     LLDGTATELTPDSSRGILHRGGTILGTSRVDPLRVEGGLDLLRRTMDTFGVEGLIVIG
                     GEGTLATGAKLWAEGVPIVGVPKTIDNDLAATDFTFGFDTAVQIATDAIDRLHTTAES
                     HARVMLLEVMGRQAGWIALYSGLAGGADVILIPEQPFDIQEVCAHLRHRHAKGHFFSI
                     VVVAEGAHPKEGGLEVPAYEQDDFGRPKLGGISTVIADEIQRRTGFETRVTILGHVQR
                     GGTPTATDRVLATRYGIAAIDLATRGTWGRMVSLRGQEIVDVSLDEAVSELKVVPPEL
                     YEVAEVFFG"
     gene            complement(14684..17803)
                     /locus_tag="VF097_10410"
     CDS             complement(14684..17803)
                     /locus_tag="VF097_10410"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6263245.1"
                     /translation="MSQQACRSCGDLLPADARFCPSCGAAVAAAEGEERKLVTVLFAD
                     VIGSTRLAARLDAERYRAVMRAFAAGAEEVVASLRGRSEKFVGDAVMAVFGVPKVNED
                     DALRAVRAAMWIRGRAERLSEDLGLEEPLRLRIGLNSGPVAAGSGDGAQLLVSGAAVN
                     LAARLQQAAEPGEILAGDTTWRLTRDAVEYGDPRVVPARGFDEPVEAHPVRSLSTRSS
                     RRTIPFVGRSRERGLLLDTFERVRETSRPHLVTILGEPGIGKSRLAEEVLAGLPEDVG
                     VLRGSAGASGDETLGPIAQMLRDELDLAGVASAEDVRARLEEMVAGCCDAGEVERTTA
                     RLGLALGLGEEDRGGHPYRVGEVRSGLEGLITGLGRRGPVALVFEDVHEAHEELLELI
                     EALVRGVRGSPLLALCLARDELLEKRSAWGAGIPDALTLRLEPLPDEEAATLARAAGD
                     ALEEPTRDRVVRLSGGNPYFIVETTGMLLERPPADGPAQWVIPPTVQAAAASRIDHLD
                     EPARRLLRTASVFPGGRFQVEHLAAITPPDPGVLESLEEAELLVPDGRPGRWRFRHEM
                     LRHVAYESLAKRERVRLHLAVADHLEAQDVHLPQAIAHHLELAARASIDLGGADPGLA
                     DRAVEALAAAGDATRRRIESRAAADLYRRGLDLAGPEAGWGVREARMLAGLGEARYWL
                     GEFEQARAALVRALELGGEDPSTQAAAGRFLADVMLNVDRDVDRATVMFETALSAARS
                     LDDPWTISRTLLMAGWAPYWRNDFDGARRSFSEALEVARKAGDAWAEARALVSLASVV
                     SSGGDQQEVLALAEEALAIGRELDEPFTVAVAQQSVGNALRRMLRLEEALPHLRAAVS
                     GFRELDARWELASTLGDLAYVHRLAGRPEEADGPLREALALCLDLGERALTTWTAAEL
                     AFLLLGQGRVEEARELLEGDVPLEDQEPALGVSARMAKALLARMEGDEEAAREHASAA
                     VAMERERGLENQIATAVYWAGSIFGPDLVGGEGAVRRARETLREAGWLHAIREAELAR
                     QPKKTSATS"
     gene            17857..18891
                     /locus_tag="VF097_10415"
     CDS             17857..18891
                     /locus_tag="VF097_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HEX6263246.1"
                     /translation="MAEIRVDLFSDTVTLPTEGMRKAIAEAEVGDEQKLEDPTVNLLQ
                     ERVADLLGAEAGLYMPSGTMCNETAFAVHCRPGDEIIADRTAHPLNAEAGGPAVLSGA
                     MVRPLDGDHGIFTADQLREAVRPPWRLSPRSRVLSVENTTNYGGGRCWPIERVREVTA
                     VAEEHGLAAHMDGARLLNAVTATGTSAAEYAEPFDSSWIDFSKGLGAPVGAVLAGSRE
                     FIEEAWVWKQRLGGAMRQAGIVAAACVYALDHHVDRLADDHEHARALAEGLAELPGVG
                     LDPETVETNIVVFELEAMPAERFAGRLLDEHGVRVSTLGPSTIRAVTHLDVDRDGVEA
                     AVVGARAILS"
     gene            complement(18884..19567)
                     /locus_tag="VF097_10420"
     CDS             complement(18884..19567)
                     /locus_tag="VF097_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263247.1"
                     /translation="MTEHTLVLEDRTTCPSCAAVNPSGSPFCWQCYAPFAQAVPSAPA
                     WARPDATGENAAPVAPARAPANPRHRWLGLALVLALAGGFFAWRVLTGGPFPGTFRGY
                     ERLDSADATRFEDGVRDLGEALGIGMHGAQYGHGSELAVLAIVADEGASPEELGSRFL
                     EGPAPRPRDLLEVQRDGATFLCFPADADVPGAGCTWIDRDSVGVVYGRFMDVEQQLEL
                     SAELRAAVS"
     gene            complement(19611..20450)
                     /locus_tag="VF097_10425"
     CDS             complement(19611..20450)
                     /locus_tag="VF097_10425"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEX6263248.1"
                     /translation="MSRPLIGAHVPARGRPTNAVDEAEARGAEAFQIFVSNPRAWAPP
                     TMSAEAAEEFRARREAAGSPPAFAHSSYLVNIAAPNPEFWRRSVELALGEARVAAALA
                     LDGLVVHAGAGGPGERAAAVARASAAATAIAEVGAPLLLELTAGGSGTVASTLHQAVD
                     LLDAAGGDPRLGIVIDTCHLFAAGAPLDDPRQAPVALDPLDVPPLSRRLRLVHANDAR
                     DPRGSRRDRHAHVGEGHIGEEGFRAILAHEAVRRVPVIIETPGKVEEDLRNIALLRRL
                     AGD"
     gene            complement(20447..22276)
                     /gene="pknB"
                     /locus_tag="VF097_10430"
     CDS             complement(20447..22276)
                     /gene="pknB"
                     /locus_tag="VF097_10430"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEX6263249.1"
                     /translation="MIETTVDGRYQILARVAAGGMGEVFRAHDPVLNREVALKMLHPT
                     LAGDTDFIDRFRREARAAAKLSHPNIVAVHDWGQAGETYFMVMEYIRGPNLRTVLADG
                     VPLQPAQAAEVLSQVLAALGHAHEHGIVHRDVKPENILITRQGVVKVADFGLARALAE
                     SRVTHAPGTVTGTVQYLAPEQIQGEPADPRTDLYAAGVVLYELLVGRVPFTGETSVAI
                     AYRHLQDPMPVPSSANPMVPTSLDEVVAHATEKDRERRVESAERMRKELASSTAELPP
                     APPLRALAEAAPSVDEVPPDRARTVTIPEALPPGRRRRRRLATFARVIALLAVLAAAG
                     WATWTYLIPHRTDAPRLQGLTIEEAQGRAEEAGLSLLVDGREYASDVEAGRILSQEPG
                     AGEVVERGTEIRVVLSRGPELVAIPEVTGQTIERARGMLRRADLTWEVNRVYHSQAPE
                     GTVIDQNPLADREIEVGTQVVLTVSRGPRPVEVPGVVGVSASEAEAELLAAGLRVTRT
                     QEFSDRVLRGEVIRQSPEAETRVLPGTAVEIVISRGPREFPMPNVIGMSREEAVATLR
                     GLGLTVDATRVPGSTGDRVVGQQPRADTTVRAGAQVTIYIGGG"
BASE COUNT         3096 a         7935 c         8329 g         2983 t
ORIGIN      
        1 cggcagatcg ccgacccggt tgtcgaactc gtggaggtcg gaggtcgtgg ggaggacccg
       61 ccgctcgcgc gtcagccacc tggcgatccg gccggtgccg gagcccacgt cgagcagccg
      121 catcccgggt tccacccagg gcgcgatgcg ccgcccgatc tcgttctcgc ggaggaacac
      181 gcgggcaagc gtagcgtgcg agggcttcca cgacgggggc gcggaggcgt ggcacgatag
      241 ccgggatgga ggcgtcagca tgagcgagcg ctgcccgcgg tgcggggggc tgaacgcgcc
      301 cgacgccgag tggtgcggcc agtgcttcac tcggctcggg gagccggagg agccggcgat
      361 cgatccggcg gtcgatccgg tggaggccgc ggcgatgctc ggcgagctgg cctcgccgga
      421 ggagcggacg gccgagcccg cgcccggggt gccctcggac caggccggcg gggagccggc
      481 gggggccccg cccggcggac ctcctgcgga cgagcagccg gccgagggtg agccgcccct
      541 ccagcccggg ccggccaccg aggccagccc cttccaggtc cgggaggacg ggctcgtctg
      601 ggtgtgccgc ctgtgcgagg cggagaatcc gctcgaggcc cacgtgtgcg cgcgctgcgg
      661 gacgcccttc ggggcgctgt tcgaggaggg gcgggaggct tccgagatcg accccgggcg
      721 ggccagcgcg ctgtcgctga tcttcccggg gctcgggcac ctcgccgcgg gccacagggt
      781 cgagggactg gcccgcatcc tgatcttcgt ctggctcatc gggacgatcg tcgtgatcgt
      841 ggccgcccgc gccggccgag gcctggggcc gctcctgggg ctcgtggtgc tctacatggc
      901 ctcggccggc gccctgtaca tgctgtcggc gatcgacgcg cgccgagcga ccgaggggga
      961 cgacccgatc gtctcgtcgc ggatcctcct ctacgggatc tccgcgctcc tgctggtcac
     1021 catcgtcttc ctcttcttct cggggccgac cctgcgcggc tgagcgggcg gtgccgcatc
     1081 cgtgcgacac tcccgccatg gccgatcgga ccgagggcga catccagatc gaggcggagc
     1141 ccggagacgt gatggaggtc ctcgtcgact tcgacgccta tcccgagtgg gcgggggtcg
     1201 agtcggcgga ggtcctcgag gaggacgacg agggccaacc cgcggccgtg gccttccggc
     1261 tgtccgcgat ggggttcgac gcgacctaca cgctcgaata cgcgttcgcg ccggacgacg
     1321 gggggatgtc gtggaccact cgcgaggccg ccggggccct gaaggacgtg gccggcgagt
     1381 acgtcctcga ggcgtcggac gacgggaccc acgtcaccta tcggctcgcc gtcgagctcg
     1441 cggtggccgt gccgtccctc cttcggaagc agggtgagaa gcggatcgtc acgaccgcgc
     1501 tggaggggct gaagcgccgc gtggaggagg ggtgagtctc gctcgccccc atcggctccc
     1561 ggcggctacc atggacgccg gatgaggatc ctgctgttca cgggcaaggg cggcgtcggg
     1621 aagacgacca tcgccgccgc caccgccgtg cgggccgccc gtgccgggcg gcgaacgctc
     1681 gtcaccagca ccgacccggc gcactcgttg gccgacgcct tcgacgtgca gatcggcgac
     1741 gcgccgcttc aggtcgagga ccgcctctgg gccgagcagg tcaacgcgca ggcccgcctg
     1801 gagtccaact ggcggaacat ccaggagtac atcatccagt tcctgaactg ggccggggtg
     1861 gacgcgatcg aggcggagga gctctcggtc atcccggggc tggacgagat cttcagcctg
     1921 accgacatga agcggcacgt ggacgtcgcc gactacgacc tcctggtgat cgactgtgcc
     1981 ccgaccgccg agacgctccg cctcctgtcg ctgcccgagg tcatgaactg gtacatcgag
     2041 cggatcttcc cggtcgagcg ccgcgtggtg aagacgatcc gcccgatcct gacccggctc
     2101 acgacgatgc cgatcgcgga ggatcgcatc ttcgcagcga tcgagcagct ccaccgcaac
     2161 ctcgaggggg tccgcgccat cctgggcgat gccgagcggt cgtccgtgcg gctcgtggtc
     2221 aacccggaga agatggtgat cgccgaggcc cgccgcacgt acacgtacct ctcgctcttc
     2281 gggtaccggg tggacgcgat cgtggcgaat cgcatcatcc cggaggaggt caacgacccc
     2341 tacttcggga agtggaagga catccaggcc gagcatctcg ccacgatcca cgagtccttc
     2401 cagccgatcc cgatcctgac ctcgcgcctg ttcggacagg agatggtggg cccggacctc
     2461 ctcgaggaga tgggggacga ggtctacggc gagggagacc cctccgcagt cctctactcc
     2521 gaggagccga tgcggatccg ccgtcggggc ccgctgtacc tcctgacgct ccgcctgccg
     2581 ttcgtggacc gaggggccct cgacatccac cggaaggggg aggagctgtt cgtgagggta
     2641 ggctcccaca aacggaacct gatcctgccg caggcgctcc agcggctcga cgtccgagac
     2701 gcccagctcg tggacgaccg cctcgagatc gcgttcgggg aggaagcgca gagccgcgcc
     2761 ggcgccggcg gcggctgacc gacggagggg gaccgatggc agagaaggag acggcgcggg
     2821 aagaggggac ggacgttccg acctgtccgg tgtcgatctg tccggtgggc atgatgctca
     2881 cgctggccgg gcaggccaag ccggaggtgg tggagcacct gctcaacgcg gggcgggagc
     2941 tgatcatggc ggccaccgtc ttcctgaacg cccgagccga tgccgtatcc gagagcccgc
     3001 gcctggagcg catcgacgtc gattgagacc ggaggatcgc ccatggcaac cctcgaccaa
     3061 ggactgctgc gcaagctcag cgactggtcc ccggcccacc cggtgacatc gctctatctc
     3121 gacgtggacg gccgccgcca tccgagcagg aacggctacc tcgtccggat gcgcgacctc
     3181 ttcaagggga tgtcgctgga cgggctcgat cgcgagcagc ggaggtccgt ggccgaggac
     3241 atggaggcga tccgccgcca cgtcgaggac gagctcgacc gcagccgtgt ggggggcgtg
     3301 gcggtcttct cgtcggcgag gagcggcctc tgggagagcg tgcccgtgtc gcgccccttc
     3361 cgcgaccggc tccacgtcgg gagccgcccg ctgctgatcc cgctcgaggg gatgctcgag
     3421 cgcttcgaga ccttctgcat gctggtcgcc gatcgggagc acgccagggt gctgtacgtc
     3481 cgccaggacc tcgtcgagga gctgggggag atccgcgacg acgtccccgg ccggcacgag
     3541 cagggtggct ggtcgcaggc ccgcttccag cgccacatcg aggaccacgt cctgcgccac
     3601 ctcaagcaca ccgcgcaggt ggcgctgcga gcgtcgcagc ggcagccctt cgatcacctg
     3661 atcctggccg ggtccgagga gctcgtcgcc gacctcgagc gtgagctcca cgactacgtg
     3721 gcacggaagg tgctcgaccg gatcgtcctg cccatcaccg tctcggctga cgaggtccgg
     3781 gagcgggtgg ccgcgatcga ggcggagctc gagtcgaggc gcgaggccga ggcgctcgag
     3841 cgtctcacga tcgaggcgag ggcagggacg gggcgggccg tggcgggcct ggcggggacg
     3901 ctcgaggccc tcgagtcggg acgcgtggag acgctcgtgg tcgccgccga cctccgagcc
     3961 gacggccggc ggtgcgctcg gtgcgggcac ctctccctgg cggaggcccg gtgcgccgcg
     4021 tgcggcgcgg acacggaggc cgccgaccac atcgtcgagg aggccgtcga gctcgccgtg
     4081 cggcagaagt gccgcgtcga gacgatcggg gccgcggagg agctcgggcc ccttggaggc
     4141 gtcggagcgc tgctccgctt ctgaggtatg ggcgagcggc ctctggcgat cggcctcgac
     4201 gtcggaggca gcaaggtctc gggcgtcctg atgtcggacg aggggtccgt gctggcctcg
     4261 gagctgctgg cgtcccccgc cgacgacgtc gaggcgatgg tcgagaccgt ggtgctggcg
     4321 gcggagcggc tgcgggcgga gggtcccgcc gcggccgtgg ggctgggcgc ggcggggatg
     4381 gtcgagcacg acaccggcgt cctccgctcc gccccgaaca tcgcgtggac ggaggtgccg
     4441 ctccgcgacc tcgtccgccg gaggatcggg cttccgtgcg tggtggacaa cgacgcgaac
     4501 accgcggcgt ggggggagtt cctgttcggc gccggtcgcg gcctgcgaca cgtcctcctc
     4561 gtcacggtgg ggacgggcat cgggggcggg gtgatccagg acgggaggct ctaccgcggc
     4621 gcccacgggt tcgcggcaga gatcggacac atcatcgtgg agccggacgg gccgcggtgc
     4681 ggctgtggga accgggggtg ctgggagcag atggcgagcg gtcaggcgct ggagcgcacg
     4741 gcccgggagg tggccctcga cgaccccgag gggcccatcg cgcgggcggc cggcgggagg
     4801 cctccggtcg gccccgacgt ggtgacggcc gccgcgggcg gggacccggc cgcggggacg
     4861 atcctcgaga ccgtggggcg ccggttgggc gagggcctgg ccgggctggc caacgttctc
     4921 gaccccgagg ccatcgtggt gggagggggg atggccgaga tcggggcccc gttcctggaa
     4981 ccggcccggg cccgcttcct ggaaacggtc gaagcgcccg accaccgtcc cgagatcccg
     5041 atcctgcagg ccgtcctcgg gaacgacgcc ggggcgatcg gggcggcggc gatggcgctt
     5101 gagttcctgg aggcggagtg aggatcggcg tcgtcctccc cctgttctcc ggagatccgg
     5161 acaaggtact cgccgcggcg cgcgagaccg aggcgctcgg gtacgacggc gcattcgtct
     5221 tcgatcactt cttcccgccc ggcgccgccc cggatcgccc gtctctcgag gccttcacca
     5281 cgctcgcggc ggtggccgcc gtcaccgagc ggatccgggt ggggacgctc gtcacgcggg
     5341 cctcgctccg tccgcccggc atcctggcga agcaggcctc gtggatcgac gcgatctcgg
     5401 gcggacgcct gatccttggg atcgggacgg gcgacgagac ggacgcagac gagcaccggg
     5461 ccttcgggct ccccttgatg ggccgcggcg agcggcgggc ccatctcctg gagacggtgc
     5521 gggccctccg gtcgatgttc gagggggagg gctactcggg cggggagagg gtgcctccgc
     5581 tctcgggccc gctcgtcccc gccgcagcgc cgccgatctg ggtcgccggg ctggccgatc
     5641 ccgtggtccg gctggcggct cgggaggccg acggctggaa cggctggggc ctggacgccc
     5701 ccgcgttcgc ccgcaaggcc gccctcctca gagcggaggc gggggatcgc accgtggagg
     5761 cgacctgggc ggggatcgtc cttgtgggcc gggacgacgc cgagctcgcc gagctgaagg
     5821 cccgccgcgc cgaccgaggc atggaggacg cctcctggag cgggaccgcg gtggagctgg
     5881 cagggttcct gacccagctg ggacgggagg gggccgggtg ggggatcttg gtggccgccg
     5941 ggccgcgcga ccgccgggag ctgatcgccc gcgaggttct cccccagctt tgacacgggt
     6001 cggagggggt ccgtactctc cctcgggccc gaggcggaag gagcatcgat ggcgaagcga
     6061 gtcgtgatca tgggggccgc ggggcgggac ttccacaact tcaacgtggt ctaccgcggg
     6121 cacccggagg tcgaggtcgt cgccttcacc gcgacgcaga tcccgggcat cgagaaccgc
     6181 cgctacccgc cggagctcgc cgggaaccgc tacgcggagg gcatcccgat ccgcatggag
     6241 gacgagctgg ccgacctcat cgcaaatgag cgggtcgacg aggtcgtctt cgcctactcc
     6301 gacgtctcgc acgagtacgt gatgcatcga gccagcgccg ccttggccgc cggcgcatcc
     6361 ttcacgctgc tcggaccgga ggcaacgatg ctggactcca cgaagccggt cgtggcggtc
     6421 tgcgcggtgc gcaccggctc gggcaagagc cagacgaccc gggcggtcgt ccgggccttg
     6481 aagacccacg gccgcaagcc cggcgtggtc cgccacccaa tgccgtacgg ggacatcgtc
     6541 gcccagcggg tccagcgctt cgccacgatg gacgacctcg acgccgccga cgtcaccgtg
     6601 gaggagcgcg aggagtacga gccgcacatc gcctccgaca ccgtcgtcta cgcgggcgtg
     6661 gactacgcgg agatcctgga agcggtccag caggaggccg acgtcgtcat ctgggacggg
     6721 gggaacaacg acctcccctt ctaccggccc gacgtccacc tcgtggtggc cgaccctcac
     6781 cggcccggtc atgagacccg gtaccacccg ggagaggcga acctccgcat gggcgacgtg
     6841 atcgtgatca acaaggtgga cactgcggag cccgagaacg tcgaggccgt cgaggccacc
     6901 gcccgcgagc tcaacccgga cgcggtgatc atccgcgcga acagccccgt gaccgtggac
     6961 gacccggacg ccgtggaggg agcccgcgtg cttgtgatcg aggacggccc caccctctcg
     7021 cacggcgaga tgacctacgg cgccggggtc gtggccgccc gccagcacgg ggctgcggag
     7081 atcgtcgacc cgcgggaatg ggcggccggc agcctgaagt cggtctacga gaagtacccc
     7141 gtgggccccg tgctccccgc gatggggtac tcggacgagc agatccgcga gctcgaggac
     7201 acgatcgacg cggttccctg cgacctcgtg atggtcgcca cgcccgtgga cctttcccgg
     7261 atcgcatcgt tctccaagcc ctccgtccgc gtgggctacg agctcgagga ggccgaggga
     7321 cagcccacga tcgccgaggt cctcgccgcg cgactgcccg cggcgggggg ctgaggtgcc
     7381 tcgcgtcgtc atcgcgctgg gcggcaacgc gctcctccag cgcgaggagg aggatacggc
     7441 ggaggtgatg gtccgcaacg cgcgcaccgc ggccgagcag gtggccgacg tggcggcctc
     7501 gggctgggag atcctcgtca cccacgggaa cggaccgcag gtcggccgca tcctcctgca
     7561 gaacgaggcc gcccgggact gggcccatcc gatgcccctc gacgtgtgcg gcgccatgag
     7621 ccaggggcag atcggctacc tccttcaggt gacgatcggg gacgtcttct acgagcgggg
     7681 gatggagcgg ccggtcacca cgctcctcac gctgacccgg gtccgggcgg acgaccctgc
     7741 gttccaggac ccgacgaagc cgatcggtcc cttctacgag ggggccgagg cggagcgcct
     7801 cgccgaggag cggggctggg tcgtgaagga ggtgggagag ggcggctggc gccgggtggt
     7861 cccgtctccc aggccctact cgatcgtgga ggccccgatc gcgaagcagc tcgcagagtc
     7921 ggagacgatc gtgatctgca ccggcggcgg cggcgttccc gtgatcgagg agggtccccg
     7981 gctggtgggg gtggaggccg tggtggacaa ggaccacgca tcggcgatca tggcgcgcga
     8041 ggtcgaggcc caggtcctca tgatcctcac cgacgtaaga ggtctgtacg aacggttcgg
     8101 cgagcccgat cagcggctgc tcgagcggct caccgtggac gaggcggagc gggtcctgga
     8161 ctccctcccc gccggctcga tgggcccgaa gctcgaggcg tgcgtggcct tcgtccgggg
     8221 cgggggagag cgggccgtga tcgggccgct ggacggtgcg ctggcggcgc tcgagggccg
     8281 catcggcacg tcgatcgtct cggcctgacg ctcgggctag gcttcccggg atggagcttc
     8341 gcgtcgtcgt gtggaacgcc catgggctcc gggcgggcgt ggccgccgcc gccgagacgc
     8401 tcgcgcgaca cgagcccgat ctggtcgtgc tcaacgaggt ggggtggagc ggatggaggc
     8461 tggcgcggct ggcccgggcg ctcgacgcgc gcgccgcgtc tgggctccgg cgcctccggt
     8521 ggggcgtgac gaacgccgtg ctcgcccggc ctccctggcg tctcctcaga actcggacga
     8581 tccgtctccc ggggcgatcc aggccgcatc ggggggtcgt cgcggccttg gtcggccggt
     8641 ccgggtaccg gctgacggcg gccagcgtcc acctcgggct ctccgatccg gagcgccgcg
     8701 agcacgcccg gggcctcacc gacaggatcc tccccgagct gcgcgccccc gttctcctgg
     8761 gcggcgatct gaacgaggcg ccggaaggcc cggcggccag atggatgacc gagcgtctct
     8821 gggacgcgtt cgcccaggcc ggccgggggc ggggtgagac cttccccgcc cgcgaccccc
     8881 gagcccgcat cgactacctg ctcgtccccg agggcgtgac ggtggcccaa gccttcgtcg
     8941 accgtgacgc ggcggaggcc tccgaccacc tgccgctcgt cgccgacctg gagctcccct
     9001 agccgcgcgt cacgcgctcg atgaacgcgg cgctccgctc gaagatggtg ggagcgtcgt
     9061 tgtccagcgc ggccacgtgg taggagtccg ggaggaggac gagctccttg tcctcgctcc
     9121 cgatccgatc caggagccat tccgccgtgc ccctcgggac gacgtggtcc tggggggagt
     9181 ggaagagcag gaggggctgg acgatcgagg ggatcaccgt ctcgagccgc ttcatcatcc
     9241 tggcgacctc cgagacggct cgaaggggca cgcgagggta gggcagctcg ttcccgcccg
     9301 gcttcttgat gtcgttgccg atccccttga tggttcgaag gaacgggcga acgaccggca
     9361 gccacacgtg ccgagggtcc cgtacatagg ggttcaccag cacgatcccc cggacgtccc
     9421 cgggacgacg ggacgccagg tcgagggcga tggtgccgcc gaaggactgg gcgcagatga
     9481 cgacccgacc cccatcccct cgaagcctcg tcagcgccgc atccgcctcg gcgacccatt
     9541 cgtccccgcg ccgggtcgcc aggtcctgcc acgtcgtccc gtgccccgga agccgcggac
     9601 cgctgaccgt gaagccgcgc tccgcgaggg cctcgcccca cggcctgatg ctcgacgggt
     9661 tgccggtgaa gccgtggagc atgagcaccg ccacgtctcc gccgggaaag aagaacggct
     9721 cggccccggg gacgatctcg gtgctcatcc cacggcggcc cgcacgcggt cgatcagcgc
     9781 ctggggatcg aacggcttca cgatgaagtt ctcgcggccc accaggccga ggacctcgtg
     9841 cccgacctcg ggcttcccgg agaccacgac gacgggggtg tcacgggtcg cgggcgccgc
     9901 acggagctgt gtgagcaggc gctcgccccc catgccgggc atcatcagat ccaggacgat
     9961 tgcatcgggg cggtgtcgct ccaagagctc gagcccttcc gccgccccgc tcgcctccac
    10021 cacgtcgaag tcgtggagct ccagggtgac gcgaaccagc tgacggaccg agtcgtcgtc
    10081 ctcgaccagg aggatcgtct tcctgagcgt cacagggtca ccgaccgcgc gggttgggtc
    10141 ggcggcggag accggtgttc cggaagggga gggccaggag gtacagggcg aagaggcccg
    10201 cagcgagcga tagggacccc accacgatca cgaacgtcca ttcgaggacg ccccatccgc
    10261 tctccacttc gacctcctcg tcgcttgtcg gccccggggg gagcctatag tctaaatgac
    10321 cgccctgaat cgaggatcga gatgttctac tggttcctca agcacgtgct gctcggcccg
    10381 ctcctccggc tgatctacag gccgaaggcc gtggggctcg agcacgtccc cgccgagggg
    10441 ccggtcatcc tggcctcgaa ccacctgtcg ttcgtggaca gcctcttcat ccccctgctg
    10501 gtgaagcgcc ccgtggtgtt cctggggaag gccgactact tcgactcgtg gaggacgcgc
    10561 tggttcatga aggcggtcaa cgtgatcccc gtgcggcgca ccgggggctc cgcgagcgag
    10621 gcggcgatcc ggacgggtgt ggaggcgctg gagaagaacc tggtgatcgg gatctacccg
    10681 gaggggaccc ggagccccga cgggcggctc tacaggggga agaccggcgt ggcccggatg
    10741 gctctcgagg ccggcgtccc cgtcgttccg atcgcggtcg acggtacggt cgacatcatg
    10801 ccgctcgaca agaagatgcc ccggctgcgg ggtcgagtga ccgtacggtt cggcgcgccc
    10861 ctcgacttct cccgctacta cgatcgcccc cgcgaccggt tcgtgctccg ctcggtcacg
    10921 gacgagatca tgtacgagat catgatgctc tcggggcagg agtacgtgga cgagtacgcc
    10981 gccaaggtca agaaggagct ggcggcccag gaggagcgga cggtcaccgt gcccgacacc
    11041 gagaccgttg accaggactc gcgcagcggc accgcctgat cagccgcggg cccaggccag
    11101 cgagcgctcc acggcccttc gccagtcgtc gtagcgcgcg gtgaggtcct cgtcgcccgg
    11161 gaggaactcc gcgtcgagct cccaggccct cgccgcctcg tccttcgacg accagacgcc
    11221 cgtggcgagc ccggccaggt acgcggcccc cagcgaggtc gtctcctgga ccttgggccg
    11281 ccgcaccggc accccgagca ggtcggcctg gaagcggcac aggagatcca tcacgctcgc
    11341 gccgccgtcg accttgagct ggctcagccc gacctccgag tcggccacca tccggtcgac
    11401 cacgtccctc gtctggaacg ccatcgactc gacggcggcg cgagcgaggt gcgcccggtt
    11461 cgtgccccgg gtgatcccca cgatcgtccc ccgcgcgtgc gggtcccacc agggagcgcc
    11521 caacccggtg agcgccggga cgaagtagac gccctcggtg tccgggacgg aggcggcgag
    11581 cgggccgatg tcggcggagg tttcgatgat cccgaggccg tcccgcagcc actggatcgc
    11641 cgcgccggtc acgaagatcg agccctccag ggcgtagcgc acgccctcct cgtccgagta
    11701 ggcgatcgtc gtcaggaggc ccttggcccg cggcgcgtgg tccccggtgt tgaggagcac
    11761 gaaggagccc gtgccgtagg tgttcttcgc ctggccgggg gtgaagcagg cctggcccac
    11821 gagcgcggcc tgctgatcgc cggccacgcc ggcgatcggg acgtggatgc cgaggaacgc
    11881 gtcgggatcg gtctcgccga aggagcccga cgacggcccg acctccggga gaacctcccg
    11941 ggggatccgc agccgctcca gcagctcggg gtcccagtcc aggcggagga tgtcgaagag
    12001 catcgtgcgg gaggcgttcg tgtggtcggt gacgtgggcc cggccgccgg tgagccggga
    12061 gatcaggtag gcgtccaccg tcccgaaagc gaggcgtccc gcctcggcgg cctcccgagc
    12121 cccctcgacc tggtcgagca gccaggccag cttcgtcccc gagaagtagg gatcgatgac
    12181 caggccggtg cgctcgatca cccgctcctc ccagccctcg tccacgagct ccgcgcacag
    12241 gggcgcggtg cggcgatcct gccagacgat cgcggggtgg acggggtgga gcgtctcccg
    12301 gtcccagagg accgtcgtct cgcgctggtt cgtgatgccg agggcggtga ggtccgaggt
    12361 ccggaggtcc gcggcgcgca gggcggcgag cgtggcttcc tgcgtggccg tccaccagtc
    12421 ctcgggctcg tgctcgaccc acccgggccg cgggaatgac tgggcgaact cccggtaccc
    12481 cgtccccagg acggagcccg cctcgttcac gaccagggcc ttcacgccgg tggtgccggc
    12541 gtcgatcgcc aggatccgcg ccacgggcga catgctagag ggggtccggc gcgggcacta
    12601 cccgcggggc acgaggatct cagcctcgac gtggctcagg ccgaccccga ggtactccgc
    12661 ggctcccagg gagaggtcga gcacccgccc ctcgatgaag ggccctcggt cgttcacgag
    12721 gacggtcgcg cagcgtcccc cgtgccgaac gtggaggaac gatgggaggg gcagggtgcg
    12781 gtgggccgcg gtgaagaggc gagggtcgaa gatggcgccg ctggcggtgg ggcgcccggc
    12841 gaagtcccag ccgtaccagg aggcgatccc ctcgaagccg gacccggtgg ggcggagccc
    12901 ggacgggatg tcgcagcccc ggccgccgtc gggtccgagg agcgcgagga gctcggactg
    12961 gtcgagcccg ccggtgatca ggggcgcgcg ggcgcgcgcg cgccgctcgg ccgcggccag
    13021 ccgccgggcc tcccgcttca gggcggcgac gcgcgcgccc gcgcgcctcg cctcctccct
    13081 ggcccgaagc agggcgaacc ccatctcctg gcgcaggccc tcgagcctcc gctggaggga
    13141 gaagagctcg cggcgccgct gctcgaggcg gcgctccacc atcgccctcg cccctcgctc
    13201 ctcgagcacg cccgccacgc gctcgacgtc gtccacgaag gtccgctgga tcgcctcacg
    13261 gacgaccagc atgtcggtga cgctaccggc ggagaggaag gcctccagga acccggccgg
    13321 tcccatctgg tacgcggccc gggcccggtc cgagagcgtg tttcgcgcgt ggtccaggtc
    13381 cgcgtcgagc cgcaggctct cgcgatagtc gtcgacgagc gcggcggcga gccgcccggc
    13441 gcgctcgtcg agcgcgtcgt agcgacgacc cagcgtggcc agccgtcccc ggagcgaggc
    13501 gacctccgag cggacggcgt cgcgctccgc ccgcgcgtcg tccagcgggt ctgcggcggc
    13561 gctcgtcccc cccaggaacg cgaggagggt ggcgaccacg gctgcgagga acctcggcat
    13621 ggtccaggga aggcatcggc gcgcgggccg catcccttga cgagccccgg ataggatgag
    13681 tcgtggccac gatcggcatc cttaacgggg gcggggactg cccgggcctc aacgccgtca
    13741 tccgcgcggt cgtccgcaag ggcgtggagg actacggcga ccggatcctc ggcttccggg
    13801 atggctaccg gggcctgctg gacgggacgg cgaccgagct gacgcccgac agctcgcggg
    13861 gcatcctcca tcgcggcggc acgatcctcg gaacctccag ggtggacccg ctgcgggtgg
    13921 agggcgggct cgatctgctc cggcgcacga tggacacgtt cggcgtcgag ggcctcatcg
    13981 tgatcggggg cgaggggacc ctggcgaccg gggcgaagct ctgggcggag ggcgtcccca
    14041 tcgtcggcgt gcccaagacg atcgacaacg acctcgccgc cacggatttc acgttcgggt
    14101 tcgacacggc cgtccagatc gccaccgacg cgatcgaccg gctccacacc acggccgaaa
    14161 gccacgcgcg cgtgatgctg ctcgaggtga tgggccgtca ggcgggatgg atcgccctgt
    14221 attcggggct ggcggggggc gccgacgtga tcctcatccc ggagcagccc ttcgacatcc
    14281 aggaggtctg cgcccatctg cgacaccgtc acgccaaggg ccacttcttc tcgatcgtgg
    14341 tcgtggccga gggggcccac cccaaggagg gcggcctcga ggtcccggcc tacgagcagg
    14401 acgacttcgg tcggcccaag ctcggcggca tctccacggt gatcgcagac gagatccagc
    14461 gccggaccgg gttcgagacg cgggtgacga tcctgggcca tgtccagcgg ggcgggacgc
    14521 ccacggcgac cgatcgcgtg ctggccaccc gctacgggat cgccgcgatc gacctggcca
    14581 cgagggggac gtggggaagg atggtctcac tgcgggggca ggagatcgtg gacgtctcgc
    14641 tagacgaggc cgtgagcgag ctgaaggtcg tgccgcccga gctctacgag gttgccgagg
    14701 tcttcttcgg ctgacgggcc agctcggcct cccggatcgc gtgcagccat cccgcttccc
    14761 gaagcgtctc cctcgcgcgc cggacggcgc cctcgccccc gaccaggtcg gggccgaaga
    14821 tgctcccggc ccagtagacg gcggtggcga tctggttctc gagcccacgc tcccgctcca
    14881 tggccaccgc ggcggaggcg tgctcccgag cggcctcctc gtccccctcc atcctggcca
    14941 gcagggcctt cgccatgcgc gcgctgacgc cgagggccgg ctcctgatcc tcgaggggca
    15001 cgtcgccctc cagcagctcc cgcgcctcct cgacccggcc ctgccccagg aggaggaacg
    15061 cgagctcggc ggcggtccac gtggtcaggg cccgctcccc caggtcgagg cagagggcga
    15121 gcgcctcgcg cagcgggccg tcggcctcct cgggccttcc cgccagccgg tggacgtacg
    15181 ccagatctcc cagggtgctg gccagctccc atcgggcgtc gagctcccgg aaccccgaca
    15241 ccgccgcccg caggtggggg agcgcctctt ccaggcgcag catccgccga agcgcgttcc
    15301 ctacgctctg ctgggccacg gccacggtga acggctcgtc gagctctcgc ccgatcgcca
    15361 gggcttcctc cgcgagcgcg agcacctcct gctggtcgcc gcccgacgac acgaccgagg
    15421 ccagcgagac gagcgccctg gcctcggccc aggcgtcccc ggccttccgg gccacctcca
    15481 gcgcctccga gaacgagcgc cgggcgccgt cgaagtcgtt ccgccagtac ggcgcccacc
    15541 cggccatcag gagggtgcgc gagatcgtcc aggggtcgtc cagcgaccgg gcggcggaga
    15601 gcgcggtctc gaacatgacg gtcgcccggt cgacgtcccg gtccacgttc agcatgacgt
    15661 cggccaggaa gcgtccggcc gcggcctgcg tcgacgggtc ctccccgccg agctcgaggg
    15721 cgcgcacgag ggccgcccga gcctgctcga actcgcccag ccagtagcgg gcctcgccca
    15781 gcccggccag catcctggcc tcccgaacgc cccacccggc ctcgggcccg gccaggtcca
    15841 ggcccctgcg gtagaggtcg gccgcggccc gggactcgat ccgacgcctg gtcgcgtcgc
    15901 ccgccgcggc gagggcctcc accgcccggt ccgcgagccc ggggtcggcc ccgccgagat
    15961 cgatcgaggc ccgggccgcc agctcgaggt ggtgagcgat cgcctgcgga aggtgcacgt
    16021 cctgcgcctc gagatggtcg gcgacggcga ggtggaggcg gacgcgctcg cgcttggcga
    16081 ggctctcgta cgcgacgtgc cggagcatct cgtgacggaa gcgccacctc cccggccggc
    16141 cgtcggggac gaggagctcg gcctcctcca gcgactccag gacccccggg tcgggagggg
    16201 tgatcgcggc cagatgctcg acctggaacc tcccgcccgg gaacaccgac gccgttcgca
    16261 ggaggcgccg ggcgggctcg tccagatgat cgatccggga ggccgcggcg gcctggaccg
    16321 tgggcgggat cacccattgg gccggcccgt ccgcgggtgg ccgctccagg agcatgccgg
    16381 tcgtctccac gatgaagtag gggttgcccc ccgagaggcg gacgacccgg tcccgcgtcg
    16441 gctcctcgag ggcgtcgccg gcggcgcgcg cgagcgtggc tgcctcctcg tcgggaagcg
    16501 gctccaggcg cagggtgagc gcgtcgggga tcccggcgcc ccaggcggat cgcttctcca
    16561 ggagctcgtc ccgcgccagg cagagggcca gcaggggcga gccccgcacg ccgcgcacga
    16621 gggcctcgat cagctcgagg agttcctcgt gagcctcgtg gacgtcctcg aagacgagcg
    16681 cgaccgggcc ccgtcgcccg agccccgtga tcagtccctc cagcccgctg cggacctcgc
    16741 ccacgcggta gggatggccg ccccgatcct cctccccgag cccgagtgcg agccccagcc
    16801 tggcggtcgt ccgctcgacc tcgccggcgt cgcagcatcc ggccaccatc tcctccaggc
    16861 gggcccggac gtcctcggcc gaggcgacgc cggcgaggtc gagctcgtcc cggagcatct
    16921 gcgcgatcgg ccccagggtc tcgtcgccgg aggcccccgc gcttccccgc aggacgccga
    16981 cgtcctcggg caggccggcc aggacctcct cggccaggcg gctcttcccg atccccggct
    17041 cgcccaggat cgtgacgagg tggggccggg acgtctcgcg cacgcgctcg aaggtgtcca
    17101 ggagcaggcc tcgttcgcgg gatcgcccca cgaaggggat cgtgcgccgg gaggacctcg
    17161 tggagagcga ccggacgggg tgggcctcga cgggctcgtc gaagccccgg gcgggcacga
    17221 cccgggggtc gccgtactcc accgcgtccc tggtcaggcg ccacgtcgtg tcgccggcca
    17281 ggatctcccc gggctcggcc gcctgctgga ggcgggcggc caggttgacg gcggcccccg
    17341 agacgagcag ctgagctccg tcgccgctgc cggccgccac gggcccggag ttcaggccga
    17401 tccggaggcg gagcggctcc tcgagcccga ggtcctcaga gagccgctcg gcccttcccc
    17461 ggatccacat cgcggcgcgc acggcccgga gggcgtcgtc ctcgttgacc ttcggaaccc
    17521 cgaacacggc catcactgcg tcgcccacga acttctcgga gcgtccccgg agcgaggcga
    17581 cgacctcctc ggcacccgcg gcgaaggccc gcatcaccgc ccggtaccgc tcggcgtcga
    17641 ggcgggccgc cagccgggtc gagccgatca cgtcggcgaa caggacggtg accagcttcc
    17701 gctcctcgcc ctcggccgcg gcgacggcgg cgccgcacga gggacagaac ctcgcgtcgg
    17761 cggggaggag atcgccgcag ctgcggcacg cctgttggct catcggaggc caagcatagc
    17821 gatgggagcg ctgggcggcg tgtatcctcg gcggtcgtgg ccgagatccg cgtggacctc
    17881 ttcagtgaca ccgtgaccct tcccaccgag gggatgcgga aggcgatcgc cgaggccgag
    17941 gtgggggacg agcagaagct cgaggacccc accgtcaacc tcctgcagga gcgcgtggcc
    18001 gatttgctgg gggcggaggc cggcctctac atgccctcgg ggacgatgtg caacgaaacg
    18061 gcgttcgccg tccactgccg gccaggggac gagatcatcg ccgaccggac ggcgcacccg
    18121 ctgaacgccg aggcgggcgg gccggccgtc ctctccggcg cgatggtccg gccgctggac
    18181 ggggatcacg gaatcttcac ggccgatcag ctccgcgagg ccgtgcgacc tccgtggcgc
    18241 ctctcgcctc gcagccgcgt cctctcggtc gagaacacca cgaactacgg cggcggccgc
    18301 tgctggccca tcgagcgcgt ccgggaggtg acggccgtgg ccgaggagca cggcctggcg
    18361 gcccacatgg atggagcccg gctcctcaac gccgtgaccg cgacgggcac ctccgcggcg
    18421 gaatacgcgg agcccttcga cagctcgtgg atcgacttca gcaagggact gggcgctccg
    18481 gtgggggctg tgctggcggg ctcccgcgag ttcatcgagg aggcctgggt ctggaagcag
    18541 cgcctgggcg gcgcgatgcg gcaggcgggg atcgtggccg cggcctgcgt ctacgcgctg
    18601 gaccaccacg tcgatcgcct ggccgacgac cacgagcacg cgagggccct ggccgagggc
    18661 ctggccgagc tccccggcgt ggggctggac ccggagaccg tggagacgaa catcgtcgtg
    18721 ttcgagctcg aggcgatgcc cgccgagcgg ttcgccggcc gcctgctgga cgagcacggg
    18781 gtccgcgtca gcaccctggg tccgtcgacg atccgggcgg tgacgcatct ggacgtggat
    18841 cgggacggcg tggaggccgc ggtggtgggg gcgcgagcga tcctcagctg acggcggcgc
    18901 gcagctccgc cgagagctcg agctgctgct ccacgtccat gaagcgtccg tagacgaccc
    18961 cgaccgagtc ccggtcgatc caggtgcagc ccgcgcccgg cacgtccgcg tccgccggga
    19021 agcagaggaa cgtcgcgccg tcacgctgca cctcgagcag gtcgcgtggg cgtggggcgg
    19081 gcccctcgag gaaccgtgag cccagctcct ccggcgaggc gccctcgtcg gccacgatgg
    19141 ccaggaccgc gagctcggat ccgtgcccgt actgggcgcc gtgcatcccg atccccaacg
    19201 cctcgccgag gtcgcgcact ccatcctcga agcgggtcgc gtccgccgag tcgaggcgct
    19261 cgtacccgcg gaaggtcccc gggaaggggc ctccggtcag aacgcgccag gcgaagaacc
    19321 ccccggccag cgcgagaacg agggcgaggc cgagccacct atgccggggg ttcgccgggg
    19381 cccgggcggg ggcgacgggc gccgcgttct cgccggtggc gtcggggcgg gcccaggcag
    19441 gggcgctcgg gacggcctgg gcgaacggcg cgtagcactg ccagcagaac gggctgccgg
    19501 aagggttcac ggccgcgcag ctcgggcagg tcgtccgatc ctcgaggacg agcgtgtgct
    19561 cggtcatacg gcgcgtagta tcggcatccc gtccgcgccc ttgagctcgg tcagtcgccc
    19621 gcgagccggc gcagcagggc gatgttgcgg aggtcctcct ccaccttccc cggggtctcg
    19681 atgatcacgg ggacgcgccg gacggcctcg tgggccagga tcgcccggaa gccctcctcg
    19741 ccgatgtggc cctcgccgac gtgggcgtgg cggtcccgac gggagcctcg gggatcccgg
    19801 gcgtcgttgg cgtggaccag gcggagccgg cgcgagagcg gcggcacatc gagaggatcc
    19861 agggcgacgg gcgcctggcg gggatcgtcg agcggggccc cggcggcgaa cagatggcat
    19921 gtgtcgatca cgatcccgag gcgggggtcg ccgccggccg catcgaggag atccaccgcc
    19981 tgatggaggg tggaggcgac cgtgcccgag ccgccggccg tgagctcgag cagcaggggc
    20041 gcgcccacct cggcgatcgc ggtggcggcg gcgctggccc gggccacggc cgcggcccgc
    20101 tcgcccggcc cgccggcgcc cgcgtggacg acgaggccgt ccagggccag cgccgccgcc
    20161 accctggcct cgcctagcgc cagctccacc gagcgccgcc agaactcggg gttgggggcg
    20221 gcgatgttca cgaggtagga agagtgggcg aacgcgggcg ggctcccggc ggcctcgcgg
    20281 cgagcgcgga actcctcggc ggcctccgcc gacatcgtcg gcggagccca ggccctcggg
    20341 ttcgacacga agatctggaa ggcctcggcg ccgcgcgcct ccgcctcgtc caccgcgttc
    20401 gtcgggcggc cgcgggccgg gacatgggcc ccgatgaggg gccggctcat cccccgccga
    20461 tgtagatcgt gacctgggcg cccgcgcgga cggtcgtgtc ggccctcggc tgctggccga
    20521 ccacgcggtc gcccgtggac cccggcacgc gcgtcgcgtc gaccgtgagc cccagcccgc
    20581 gcagggtggc caccgcctcc tcgcgtgaca tcccgatcac gttgggcatg gggaactcgc
    20641 ggggacctcg ggagatcacg atctcgacgg ccgtcccggg gaggacccgc gtctcggcct
    20701 ccggggactg gcggatcacc tccccccgaa gcacgcggtc ggagaactcc tgggtccggg
    20761 tgacccggag gccggcggcc aagagctcag cctcggcctc cgaggcggac acgccgacca
    20821 cgcccgggac ttcgacgggg cgcgggcctc ggctgaccgt cagcacgacc tgggtcccca
    20881 cctcgatctc gcggtcggcg agcgggttct gatcgatcac cgtcccctcg ggcgcctgcg
    20941 agtggtagac gcggttcacc tcccaggtga gatcggcgcg ccgcagcatc ccgcgggccc
    21001 gctcgatggt ctgtcccgtg acctcgggga tggccacgag ctcggggccg cgggagagca
    21061 ccacgcggat ctcggtgccg cgctccacga cctcgcccgc tcccggctcc tgcgacagga
    21121 ttcggcccgc ctcgacgtcg gacgcatact cgcgcccgtc caccaggagc gacagccccg
    21181 cttcctcggc tcggccctgt gcctcctcga tcgtcagccc ctggaggcgg ggcgcgtcgg
    21241 tgcggtgggg gatcaggtac gtccacgtcg cccaccccgc ggcggccagc acggcgagga
    21301 gggcgatcac gcgggcgaac gtggcgaggc gccgccgccg ccgccgcccc ggcgggaggg
    21361 cctctgggat cgtcacggtc cgggctcggt ccggcggcac ctcgtcaacg gagggcgcgg
    21421 cctcggccag cgcccgaagc gggggcgcgg gaggcagctc ggcggtggag ctcgcgagct
    21481 ccttccgcat ccgctccgcg ctctccacgc ggcgctcccg gtccttctcc gtggcgtgcg
    21541 ccacgacctc gtccagggag gtcgggacca tcgggttggc gctcgagggg accggcatcg
    21601 ggtcctgcag gtgccggtag gcgatcgcaa cagaggtctc gccggtgaac gggacccgac
    21661 cgaccagcag ctcgtagagg acgaccccgg ccgcgtagag gtcggtgcgc gggtcggccg
    21721 gctccccctg gatctgctcg ggcgccaggt actgcacggt gccggtgacg gttcccggcg
    21781 cgtgcgtcac cctggactcg gccagtgcgc gggccagccc gaagtcggcc accttcacca
    21841 cgccctgcct cgtgatgagg atgttctcgg gcttgacgtc ccgatggacg atgccgtgct
    21901 cgtgggcgtg cccgagagcg gcgagcacct gggagagcac ctcggcggcc tgggccggct
    21961 ggagcggcac gccgtcggcc aagaccgtcc ggaggttggg gccccgaatg tactccatca
    22021 ccatgaagta ggtctcgccc gcctgccccc aatcgtggac ggccacgatg ttcggatggg
    22081 agagcttcgc cgcagcgcgc gcctcccgcc ggaaccgatc gatgaagtcg gtgtcccccg
    22141 cgagggtggg gtgcagcatc ttgagcgcga cctcgcggtt caggacggga tcgtgcgccc
    22201 gaaagacctc gcccatgccg ccggcggcga ccctggcgag gatctggtag cgtccgtcta
    22261 cggtcgtctc gatcatctcg gggggagcgg cctcgtctcc gcattgtagg gggtcatgcc
    22321 cgactaccag ggaaggctcg acc
//
LOCUS       DASUTZ010000243         2652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1772735, whole genome shotgun sequence.
ACCESSION   DASUTZ010000243 DASUTZ010000000
VERSION     DASUTZ010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1772735"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1293)
                     /gene="dnaG"
                     /locus_tag="VF097_10435"
     CDS             complement(<1..1293)
                     /gene="dnaG"
                     /locus_tag="VF097_10435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX6263250.1"
                     /translation="MARIRQDDVEAVRERTDIVRLIQQHVALKKAGRSFTGLCPFHQE
                     KSPSFTVDPGKGVYYCFGCGKGGNAFHFVMEVEQLTFPEAVERLAGPAGVTLRYEGGG
                     REDRRERSRRDALFRANDRAADLYHRMLMDGREGEEARRYLASRGITKESAERFRIGY
                     APGYPDFLLRRLGRELSPEILVEAGVAMNDRGGGVRDRFRARVTFPIADLSGRSVGFG
                     ARLLQGEGPKYLNSSETPVYRKGELLYNLDRAKGEIGRSGTAHVVEGYTDVIALAQGG
                     VETAVATCGTALSEGHFRLLSRFATRMVLSFDSDEAGARAAERAAGLFEAYPLEVAVL
                     VLPEGLDPADLVKERGADAFRDLAEGAVPLVEYMLRRSLRDADLESPEGRSRAVRSAL
                     PIVAGLDDLVRREQYAGMLADLTGVTTSTVLLELERRGD"
     gene            complement(1309..2310)
                     /locus_tag="VF097_10440"
     CDS             complement(1309..2310)
                     /locus_tag="VF097_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEX6263251.1"
                     /translation="MGSIRERQEDLERRTLSDRAALAAESKGREREESPHHMRTAFQV
                     DRDRIVHSKAFRRLKHKTQVFISPEGDHYRVRLTHTLEVTQIARTIARALRLNEDLTE
                     AIALGHDLGHTPFGHLGEEALTPFLDRPFHHSEQSLRVVDHLERDGRGLNLTWEVRDG
                     ILGHPWKMPPPATLEGWTVRMADRIAYVNHDIDDAIRAGLLTEESLPRDVTSVLGAGH
                     GDRIETLIDDIAAQGVESGEVGMSTPIAEALDALRDFMFERVYLRPESRGEQEKAIGV
                     IRALFEHYLEHPEEVPAEYAEAPGGPAARVADYIAGMTDRYALRTYERLFLPQEWML"
     gene            complement(2352..>2652)
                     /locus_tag="VF097_10445"
     CDS             complement(2352..>2652)
                     /locus_tag="VF097_10445"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX6263252.1"
                     /translation="AYLVSQGIPDSAVDPSPVGRTSLESLEAASRYMEAEALQSAFLV
                     SDPWHNLRIRRMARDLGIEAYVSATFQSAARSQGTRLAGYVRETFAYLFYRLTGS"
BASE COUNT          373 a          948 c          947 g          384 t
ORIGIN      
        1 gtcgccgcgc cgctccagct cgagcaggac ggtcgaggtc gtgacccccg tgaggtccgc
       61 cagcatcccc gcgtactgct cccgacggac gaggtcgtcc aggccggcca cgatcgggag
      121 ggcgctccgg acggcccgcg aacgcccctc cgggctctcg agatccgcgt cccgcagcga
      181 gcggcgcagc atgtactcga cgagcggcac ggccccctcc gccagatccc ggaacgcgtc
      241 ggcccctcgc tccttcacca ggtcggcggg gtcgaggccc tcggggagga ccaggacggc
      301 cacctcgaga gggtaggcct cgaacagacc ggcggcccgc tcggcggcgc gggccccggc
      361 ctcgtcggag tcgaaggaca ggaccatgcg cgtggcgaac cgcgagagga gccggaagtg
      421 cccctccgag agggcggtcc cgcaggtggc cacggccgtc tcgaccccgc cctgggccag
      481 cgcgatcacg tccgtgtagc cctccaccac atgggccgtg ccggacctgc cgatctcgcc
      541 cttggcccgg tcgaggttgt agaggagctc gcccttccgg tagacgggcg tctccgagga
      601 gttcaggtac ttggggccct cgccctggag cagtcgcgcg ccgaacccca cgctgcggcc
      661 ggacaggtcg gcgatcggga acgtgacccg ggctcggaac cggtcgcgca cgccgccgcc
      721 ccgatcgttc atggccaccc ccgcctccac caggatctcc ggggagagct cgcgtcccag
      781 gcggcgcagg aggaagtccg gatagccggg cgcgtagccg atgcggaagc gctcagccga
      841 ctccttcgtg atgccgcgcg aggcgaggta gcggcgggcc tcctcgccct cgcggccgtc
      901 catcagcatc cggtggtaga ggtccgccgc gcggtcgttg gcgcggaaga gcgcgtcgcg
      961 ccgcgagcgc tcgcggcgat cctcgcggcc gcctccctcg tagcgcagcg tgacgccggc
     1021 cgggcccgcc agccgctcca cggcctcggg gaaggtcagc tgctcgacct ccatcacgaa
     1081 gtggaaggcg ttgccgccct tgccgcaccc gaagcagtag tagacgccct tcccggggtc
     1141 cacggtgaag gacgggctct tctcctggtg gaaggggcag agcccggtga acgaccgccc
     1201 ggccttcttc agggcgacgt gctgctggat cagccggacg atgtcggttc gctcccggac
     1261 cgcctcgacg tcatcctgcc tgatgcgcgc cacgcgctct catccccctc atagcatcca
     1321 ttcctgggga aggaagagtc gctcgtaggt ccgcagcgcg tatcggtcgg tcatcccggc
     1381 aatgtagtcg gcgactcggg cggccgggcc tcccggcgcc tcggcgtact cggccgggac
     1441 ctcctcggga tgctcgaggt agtgctcgaa cagcgcccgg atgaccccga tcgccttctc
     1501 ctgctcgccc cggctctcgg ggcggaggta gacccgctcg aacatgaagt cgcggagcgc
     1561 gtcaagcgcc tccgcgatcg gcgtcgacat ccccacctcg cccgactcga cgccctgggc
     1621 tgcgatgtcg tcgatgagag tctcgatccg gtcgccgtgc ccggccccca gcaccgaggt
     1681 gacgtcccga gggagcgact cctcggtgag caggcccgcg cggatcgcgt cgtcgatgtc
     1741 gtggttgacg taggcgatgc gatcggccat gcggacggtc cagccttcga gcgtggcggg
     1801 aggcggcatc ttccagggat ggcccaggat gccgtctcgg acctcccagg tcaggttcag
     1861 cccccgcccg tcgcgctcca ggtggtccac gacgcgcagg ctctgctcgc tgtggtggaa
     1921 gggccggtcc aggaacggcg tgagcgcctc ctcgccgaga tgcccgaacg gcgtgtgacc
     1981 gaggtcgtga ccgagggcga tggcctcggt gaggtcctcg ttgagtcgga gggcccgggc
     2041 gatcgtccgg gcgatctgcg tgacctcgag cgtgtgggtg aggcggacgc ggtagtgatc
     2101 cccctcgggc gagatgaaga cctgcgtctt gtgcttcagg cggcggaagg ccttggagtg
     2161 gacgatgcgg tcccggtcga cctggaaggc ggtccgcatg tggtggggcg actcctcccg
     2221 ctcccggccc ttggactcgg cggccagcgc cgcgcgatcc gatagcgtcc gacgttccag
     2281 atcctcctga cgctcacgga tcgaccccac gggccgaagg gtagcacgag cctccgaccc
     2341 ctcctccggg ctcaggagcc ggtcagccgg tagaagaggt acgcgaacgt ctcgcgaacg
     2401 tagccggcca gccgcgtccc ctggctcctg gccgccgact ggaacgtggc cgagacgtag
     2461 gcctcgatgc cgaggtcccg ggccatccgg cggatcctga ggttgtgcca agggtccgag
     2521 acgaggaacg cgctctggag ggcctcggcc tccatgtacc gggaggccgc ctccaggctc
     2581 tccagggagg tgcggccgac cggcgagggg tccacggccg agtccgggat cccctgggac
     2641 acgaggtagg cc
//
LOCUS       DASUTZ010000244         3032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1904099, whole genome shotgun sequence.
ACCESSION   DASUTZ010000244 DASUTZ010000000
VERSION     DASUTZ010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1904099"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1510)
                     /locus_tag="VF097_10450"
     CDS             complement(<1..1510)
                     /locus_tag="VF097_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX6263253.1"
                     /translation="MPRRALTLLLSVSFLVLSGASARADVTCVFDEAVMAVQLSAPIV
                     PISVVRSGMEIVVLDGDDPVDCGTPAPTVGTTDEIQVTDLTPGSSAFTIDLSGGPFES
                     SGSKLAFDVALGGGSSDALLVNGSSSADHMTLAGAGIDLDATSDPGPHVALSGVELVV
                     VDGAGGDDTLDAGDPIDPFLRVVELRGGDGADTLIGGDAGDLLVGGGGADDLLGGDGP
                     DVLRGGAGIDSLDGGQGDDLLAGGPGDDEEAGGPGNDIFDQGTAEDGEDDLDGGAGQL
                     DVVAYDLRTEAVTVVLDGPSVSGEAGEGDAVAGIEVALGGAGDDSLTGAGGDDSLRGG
                     PGQDVLVGGGGDDYLAGGPGQDHLNGGVGDDTASFFGASGPVEASLADGVAVTEGEQD
                     TLQGIQNLDGSRHADVLVGDDGPNMLMGRGGADRLIGGGGEDTLDGGDQHDDLRGGVG
                     NDLLHGREGADLLQGGSGNDALRGGPGRDTASFRTSSSGVRASLAAGSATGQG"
     gene            1622..2014
                     /gene="sdhC"
                     /locus_tag="VF097_10455"
     CDS             1622..2014
                     /gene="sdhC"
                     /locus_tag="VF097_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEX6263254.1"
                     /translation="MVRRTKEYGSLYRGREGQWTWLAHRVTGVGVLLFLFAHIVDTAL
                     VGWGPDTYNRVIAIYHNPIVELMELGLVAAVIFHALNGVRIMIIDFWPQTATRHRVLS
                     WGTLALFVAAMAPITYLMGRRILEGIFG"
     gene            2011..2427
                     /locus_tag="VF097_10460"
     CDS             2011..2427
                     /locus_tag="VF097_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase hydrophobic membrane
                     anchor subunit"
                     /protein_id="HEX6263255.1"
                     /translation="MSVEAEPRPARAEPRPRPRSGFELWAWLFMRLSGILLLFLAVGH
                     VLIMHVIDEGVQRVDFDFVADRWASPFWRTWDWMLLSLALLHGINGTRVVIQDHVRSP
                     GWRLTWNSIFVIVGFALFVLGSVIVFTFDAAEFSFS"
     gene            2424..>3032
                     /locus_tag="VF097_10465"
     CDS             2424..>3032
                     /locus_tag="VF097_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017823808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6263256.1"
                     /translation="MSTSHEFDVVIVGAGGAGLRAALEASGAARTAVISKLYPTRSHT
                     GAAQGGMCAALANVEEDYPEWHAFDTVKGGDYLVDQPAAEIMANEAVETVIELEHWGF
                     PFNRTPDGRIDQRRFGGHTRNHGEAPVRRACYAADRTGHMILQTLYQQCIKRDVRFFN
                     EFYVLDLLMEEGRASGVVAYELATGELHVFRAKAVVIATGGYG"
BASE COUNT          406 a         1142 c         1030 g          454 t
ORIGIN      
        1 cgccctggcc ggtcgccgat cccgccgcga ggctcgcccg gaccccgctc gaggaggtgc
       61 ggaagctcgc cgtgtcgcga cccgggcctc cgcggagcgc gtcgttcccc gagccaccct
      121 ggaggaggtc ggccccctcc cgcccgtgca gaaggtcgtt ccccaccccg ccccgcaggt
      181 cgtcgtgctg gtccccaccg tcgagggtgt cctcgccccc tcctccgatg agtcgatccg
      241 cgccgccccg tcccatgagc atgttcggcc cgtcgtcccc gaccaggacg tcggcgtggc
      301 ggctcccgtc caggttctgg atcccctgca gcgtgtcctg ctcaccctcc gtcacggcca
      361 cgccgtcagc caggctcgcc tccacgggcc cggaggcgcc gaagaagctc gccgtgtcgt
      421 cccccacccc tccgttcagg tggtcctggc ccgggccgcc cgccaggtag tcgtctccgc
      481 caccgcccac gaggacgtcc tgccccggtc ctcctctcag cgaatcgtcg ccccccgctc
      541 ccgtgagcga atcgtcaccg gctcccccca gggccacctc gatccccgcc acggcgtcac
      601 cctcgcccgc ctcgccgctc accgaggggc cgtcgaggac aacggtcacg gcctcggtcc
      661 ggaggtcgta ggcgacgacg tcgagctggc ccgccccgcc atcgaggtcg tcctccccgt
      721 cctcggcggt cccctggtcg aagatgtcgt tgccggggcc gcccgcctcc tcgtcgtcgc
      781 ccgggccgcc cgcgagcagg tcgtcgccct ggcccccgtc gagcgagtcg atcccggcgc
      841 ccccccggag cacgtccggg ccatcgcctc ccaggaggtc gtccgccccg ccgccgccga
      901 cgaggaggtc gccggcgtcc cccccgatca gcgtgtcggc gccgtcgccg ccccggagct
      961 cgaccacgcg gaggaacgga tcgatcgggt ctccggcgtc gagcgtgtcg tcgcctcccg
     1021 cgccatccac gacgacaagc tcgacgccgg agagggccac gtggggtccc ggatcggatg
     1081 tcgcgtcgag atcgatcccc gcgccggcca gggtcatgtg atccgcggag gacgaaccgt
     1141 tcacgaggag ggcgtccgag ctgcccccgc cgagggcgac gtcgaaggcg agcttagatc
     1201 ccgaggactc gaacggcccc ccgctgaggt cgatcgtgaa ggcactgctt cccggcgtga
     1261 ggtccgtgac ctgtatctcg tcggtcgtgc ccaccgtggg ggcgggggtc ccgcagtcga
     1321 cgggatcgtc gccgtcgagg acgacgatct ccatccccga tcgaacgacg gagatgggga
     1381 cgatcggggc cgagagctgg acggccatca ccgcctcgtc gaagacgcag gtgacgtccg
     1441 cccgagcgct cgccccggac aggaccagga acgaaaccga gaggaggagg gtgagagccc
     1501 ggcgaggcat cgtggggcgc catgctaggg gagccacccc agtgggtcaa cgggatggga
     1561 tctctcgttc cggaccgggg ggattggcgg gggccacccc gcttcgctac cctcgactgc
     1621 cgtggtcagg cgcaccaagg agtacgggag cctctaccga gggcgcgagg gtcagtggac
     1681 ctggctcgcg cacagggtca ccggggtggg cgtgctgctc ttcctgttcg cccacatcgt
     1741 ggacaccgcg ctcgtcgggt ggggacccga cacctacaac cgagtgatcg ccatctacca
     1801 caacccgatc gtcgagctga tggagctcgg cctcgtcgcg gccgtgatct tccacgcgct
     1861 caacggggtc cggatcatga tcatcgactt ctggccccag acggcgaccc gccaccgcgt
     1921 gctctcgtgg gggacgctgg ccctgttcgt cgccgccatg gccccgatca cctacctgat
     1981 gggccggcgg atcctggagg ggatcttcgg gtgagcgtcg aggccgagcc ccgcccggcg
     2041 cgcgcggagc cccgcccccg cccgcgctcc ggcttcgagc tctgggcctg gctgttcatg
     2101 cggctgtcgg ggatcctcct gctgttcctc gccgtcggtc acgtcctgat catgcacgtg
     2161 atcgacgagg gggtgcagcg ggtcgacttc gacttcgtcg cggaccggtg ggccagcccc
     2221 ttctggcgga cctgggactg gatgctgctc tcgctcgccc tcctccacgg catcaacggc
     2281 acccgggtcg tgatccagga ccacgtgcgc tcgcccggat ggcggctgac ctggaactcg
     2341 atcttcgtca tcgtggggtt cgcgctcttc gtgctgggca gcgtgatcgt gttcacgttc
     2401 gacgccgcgg agttcagctt ctcgtgagca cgagccacga gttcgacgtc gtgatcgtgg
     2461 gcgccggagg cgccgggctg cgggccgccc tggaggcctc gggggccgcg cggaccgcgg
     2521 tgatctccaa gctgtacccc acccgatcgc acacgggcgc ggcccagggg gggatgtgcg
     2581 ccgcgctggc caacgtcgag gaggactacc ccgagtggca cgccttcgac accgtgaagg
     2641 gcggcgacta cctcgtggac cagcccgccg ccgagatcat ggcgaacgag gccgtggaga
     2701 ccgtgatcga gctcgagcac tggggcttcc ccttcaaccg gaccccggac ggcaggatag
     2761 accagcgccg cttcggcgga cacacccgca accacggcga ggcgccggtc cggcgggcct
     2821 gctacgccgc cgaccgcacc ggccacatga tcctccagac cctctaccag cagtgcatca
     2881 agcgggacgt tcgcttcttc aacgagttct acgtgctcga cctgctgatg gaggagggcc
     2941 gggcctccgg ggtcgtggcc tacgagctgg ccaccggcga gctccacgtg ttccgggcca
     3001 aggccgtggt catcgccacc ggcggctacg gg
//
LOCUS       DASUTZ010000245         4189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1904445, whole genome shotgun sequence.
ACCESSION   DASUTZ010000245 DASUTZ010000000
VERSION     DASUTZ010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1904445"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..96
                     /locus_tag="VF097_10470"
     CDS             <1..96
                     /locus_tag="VF097_10470"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006750002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEX6263257.1"
                     /translation="GEGSGALLAVPLVRAAAAAVTDVATFEEWGL"
     gene            93..902
                     /locus_tag="VF097_10475"
     CDS             93..902
                     /locus_tag="VF097_10475"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEX6263258.1"
                     /translation="MSPGRGLRGALAFLTRAPVDRGHGAREVAGSVPWFPVAGALVGL
                     LVAGVYAAGVSVLPSLPAAVVAVAAGVLATGALHEDGLADLADALGARGPDDARRIMK
                     DPTHGTFGVLALTLSVAARAAAIASLGPWAALAWVPAAHAASRGASVGLLARARAGPR
                     LEGLGPEEAHRGETGGLGVGTAAHLSEWEGSAALSIGLAVSALGVGFWAVPVGALVLA
                     ATAGLGWLAARRLGGVTGDVIGAAQQVGEVLVLLLGAAAVTQGWPGAVWWR"
     gene            902..1432
                     /locus_tag="VF097_10480"
     CDS             902..1432
                     /locus_tag="VF097_10480"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX6263259.1"
                     /translation="MVEVPPGVRLWLVRHGSTAWSEEGRLCGRSDVPLSPRGRLQAQA
                     LRDAAARLTPAGVWSSDLRRARETADLAGLSAVSDGRLRELDFGSLEGARWDDLPAAV
                     REAMLAFDGFQAPGGESVWDLARRVEGLLDELAPGLHVLVTHGGVIRLLMRGRPEAHV
                     APGGAIRLEAQPIARS"
     gene            complement(1411..1863)
                     /locus_tag="VF097_10485"
     CDS             complement(1411..1863)
                     /locus_tag="VF097_10485"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6263260.1"
                     /translation="MKEPTTMEPVRVAVTVPTRPDDAFRLYTERIAEWWPLETHSVFQ
                     QDATGAVFESREGGRIYEVHRDGRESEWGRVLVWDPPARVVYSWQPNPERSGTTEVEV
                     RFHEVDGGTRVELEHRGWERLGDEAEALRGEYEGGWVTVLDAYERAMG"
     gene            complement(1860..2189)
                     /locus_tag="VF097_10490"
     CDS             complement(1860..2189)
                     /locus_tag="VF097_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020521453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX6263261.1"
                     /translation="MTANQVLEALGDRTRQAILDILLDGPRSVAQLSERLPVSRPAVS
                     QHLRVLAQAGLVGYTTRGTRHLYHVHPEGLAELRAYVEQFWARALAAFEAAAEQGFSE
                     ERKERSR"
     gene            complement(2363..3244)
                     /locus_tag="VF097_10495"
     CDS             complement(2363..3244)
                     /locus_tag="VF097_10495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6263262.1"
                     /translation="MTERAYEHDETRVASGDVAVAAGDSMRLFLNEIGRYPLLTADEE
                     KELAKLVEEGDQEAKDRMIRSNLRLVVSVAKKYQWAGLPLLDLIQEGILGLIRAVEKF
                     DWRKGFKFSTYATWWIRQAISRAIDNQSRTIRLPAHLAQRELKISRARRQLEEELGRE
                     PTLEETSEASGIDAEEIERVRDAARAVTSLERPVDAEGETELGDLVAGENAEIEEEVH
                     VRLRDTSVRRAVASLSDTQREVVALRYGMSGEDPLSYREIAKRMGMSHEAVRRVERDA
                     LKNLAVLREIEALEEAV"
     gene            3580..3795
                     /locus_tag="VF097_10500"
     CDS             3580..3795
                     /locus_tag="VF097_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263263.1"
                     /translation="MARDDMRRDRVGETIRDDLREPDRQHLDEQTASGLPAGGPGRSK
                     MENLRRIRSQRETQRRRLHEADPEARV"
     gene            3943..4143
                     /locus_tag="VF097_10505"
     CDS             3943..4143
                     /locus_tag="VF097_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017540722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX6263264.1"
                     /translation="MATGTVKWFSPDKGYGFISQEDGPDVFVHFSAIQGDGYRNLEEN
                     QQVEFELTDGPKGPQASNVRPL"
BASE COUNT          526 a         1512 c         1525 g          626 t
ORIGIN      
        1 ggggagggaa gcggggccct gctcgcggtg cccctggttc gcgccgccgc cgcggccgtc
       61 accgacgtgg ccaccttcga ggagtggggc ctgtgagccc cggccgaggg ctccgggggg
      121 cgctcgcctt cctgaccagg gccccggtcg atcgcggcca cggcgctcgg gaggtggccg
      181 gatcggtccc gtggttcccg gtggcggggg cgctcgtcgg gctgctcgtg gcgggcgtgt
      241 acgcggcggg ggtgtcggtg ctcccatccc tgccggcggc ggtcgtggcc gtggccgcgg
      301 gtgtcctcgc cacgggcgct ctccacgagg acgggctggc cgacttggcc gacgcgctgg
      361 gcgcgcgggg ccccgacgac gcccgccgga tcatgaagga cccgacgcac gggaccttcg
      421 gagtcctcgc cctgaccctg tcggtcgcgg ctcgtgcggc cgcgatcgcc tcgctcggcc
      481 cgtgggcggc gctggcctgg gtgccggccg cgcacgccgc gagcagggga gcgtccgttg
      541 gcctgctcgc tcgcgcccgg gcaggtcctc gtctcgaggg tctcggtccc gaggaggccc
      601 atcgcggcga gacgggcggc ctcggggtcg ggacggccgc ccatctctcc gagtgggagg
      661 ggtcggcggc gctgagcata ggtctggcgg tgtcggcgct cggcgtcggc ttctgggccg
      721 tccccgtcgg cgcgctcgtg ctggcggcga ccgccggcct cgggtggctc gccgcccgca
      781 ggctgggcgg ggtgaccgga gacgtgatag gggcggcgca gcaggtgggg gaggtgctcg
      841 tgctgctgct cggcgccgcg gccgtcaccc agggctggcc gggagccgtc tggtggcggt
      901 gatggtcgag gtcccccccg gtgtccggct ctggctcgtc cgccacgggt ccacggcctg
      961 gagcgaggag ggccgcctgt gcggccggtc cgatgtgccg ctcagcccgc ggggccgtct
     1021 ccaggcccag gccctccggg acgcggcggc gcgtctcacc ccagcggggg tgtggtcgtc
     1081 ggacctccgc cgggcgcggg agaccgccga cctggcgggg ctgtccgccg tctccgacgg
     1141 gcggctcagg gagctcgatt tcggctcgtt ggagggggcg cgatgggacg acctccccgc
     1201 cgccgtccgc gaggccatgc tcgcgttcga cggcttccaa gcgcccggcg gggagtcggt
     1261 gtgggacctc gcccggaggg tcgagggcct cctggacgag ctggcgccgg ggctgcacgt
     1321 cctcgtcacg cacggaggcg tgatcaggct gctgatgcgg ggtcgcccgg aggcgcacgt
     1381 cgctccgggc ggggcgatcc gtctggaggc tcagcccatc gcgcgctcgt aggcatccag
     1441 cacggtcacc cagcccccct cgtactcgcc gcggagtgct tcggcctcgt ccccgaggcg
     1501 ctcccatccg cggtgctcga gctcgacgcg ggtcccgccg tcgacctcgt ggaatcggac
     1561 ctcgacctcg gtcgtccccg accgctccgg attcggctgc cacgagtaca cgacccgggc
     1621 cggcggatcc cagacgagga cgcggcccca ctcggactcg cgcccgtcgc ggtgcacctc
     1681 gtagatgcgc ccgccctccc ggctctcgaa gaccgccccg gtcgcgtcct gctggaagac
     1741 ggaatgcgtc tcgagcggcc accactcggc gatccgctcc gtgtagaggc ggaacgcgtc
     1801 gtcggggcgc gtgggcaccg tcactgcgac acggaccggt tccatcgtgg tcggctcctt
     1861 catcgtgatc gctccttccg ctcctcagag aacccctgct cggcggcggc ctcgaacgcg
     1921 gccagcgcgc gggcccagaa ctgctcgacg taggcccgca gctcggccag tccctcggga
     1981 tggacgtggt agaggtggcg cgtcccgcgg gtcgtgtagc cgacgagccc ggcctgtgcc
     2041 aggacgcgca ggtgctggga gacggcgggc ctgctcacgg gcaggcgctc cgagagctgc
     2101 gcgaccgatc gggggccgtc cagcaggatg tcgaggatgg cctggcgggt cctgtcgccg
     2161 agcgcctcga gcacttggtt cgccgtcacg aacggtaagt tatcgcgaac ggtaagtcga
     2221 tgtcaacgga aggtcgggcc cgcgaggaac ggaacacgag gaacagaaac gggcgcgccc
     2281 ccgagagggg gcgcgcccgt tcacatggtg gccgggccct ggaacgacct accctagccc
     2341 tcctctcgac gggagggcaa ggtcagaccg cctcctccag ggcctcgatc tcccgcagga
     2401 cggccaggtt cttcagcgcg tcacgctcca cgcgcctgac ggcctcgtgc gacatgccca
     2461 tccgcttggc gatctcccga tacgagagcg gatcctcgcc cgacatcccg tagcggagcg
     2521 cgaccacctc gcgctgcgtg tccgagagcg aggcaaccgc ccgccgcacg ctcgtgtcgc
     2581 gtagccgcac gtggacctcc tcctcgatct ccgcgttctc gcctgcgacg aggtcgccca
     2641 gctcggtctc gccctccgcg tccaccggac gctccaggct ggtcacagcg cgggccgcgt
     2701 cgcggacgcg ctcgatctcc tcggcgtcga tgcccgaggc ctcggacgtc tcctcgaggg
     2761 tcggctcccg gcccagctcc tcctcgagct ggcgcctggc ccgcgagatc ttcagctcgc
     2821 gctgggcgag gtgcgccggc agccggatcg tgcgcgactg gttgtcgatc gcgcgggaga
     2881 tggcctgccg gatccaccag gtcgcgtacg tggagaactt gaagcccttc cgccaatcga
     2941 acttctcgac cgcgcggatc aggccgagga tcccctcctg gatcaggtcc aggaggggga
     3001 ggcccgccca ctggtacttc ttggcgaccg agacgaccag ccggaggttc gaccggatca
     3061 tccggtcctt cgcctcctgg tcgccctcct cgacgagctt ggcgagctcc ttctcctcgt
     3121 ccgcggtcag cagggggtac cggccgatct cgttgaggaa cagccgcatg ctgtcgccgg
     3181 cggccacggc cacatcgccg ctcgccaccc tcgtctcgtc atgttcgtat gcacgctcgg
     3241 tcatgggtct ccaaacgtcg tcgaaacggc ccctctccca tgctctccac gcgaggggcc
     3301 ttgaaacctg cgccgcgggg ggacgccttc ctcatctatc ttcgactcga tccgtccaac
     3361 ggttcagtgg tggcccgtat tcccgacgcc gaacggccga tcgcggttcc gccgcccggc
     3421 tcgacgccga ggcgtgcctg ccaaacccgg acaatggaga cgagtccctc cgggggctgc
     3481 ctcggtgcgc gtccccgccg cccagtacct ctccacctcg gtgatgtcga actccccgac
     3541 ctgagtgtcg gtctcacggc cgcggggtac gcatcgggca tggctcgcga cgacatgcgc
     3601 cgggatcggg tcggcgagac gatccgggac gacctgcggg agccggaccg gcagcacctc
     3661 gacgagcaga ccgcgtccgg gctccccgcg ggcggcccgg gccggtcgaa gatggagaac
     3721 ctccggagga tccgcagtca gcgcgagacc cagcggcgcc gcctccacga ggccgacccg
     3781 gaggcccggg tctaggcccg agagcccctc actggtagac tctgggtgag ggttccgcat
     3841 gtagtttcac gcttcaggca gttccctcac cggcgcggtg gcgccggggg ccgacgggcg
     3901 agcagttcac gtccgatccg gccacgaaag gagggaacgc ttgtggcaac aggaaccgtg
     3961 aagtggttca gccccgacaa gggctacggc ttcatctccc aggaggacgg ccccgacgtc
     4021 ttcgtgcact tcagcgcgat ccagggcgac gggtaccgca acctcgagga gaaccagcag
     4081 gtcgagttcg agctgaccga tggaccgaag ggtccccagg cctcgaacgt gcgcccgctc
     4141 tgagggcggc gatctagagg ggggcggcgc gcgtggcttt cgccacgcg
//
LOCUS       DASUTZ010000246         7773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_350280, whole genome shotgun sequence.
ACCESSION   DASUTZ010000246 DASUTZ010000000
VERSION     DASUTZ010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7773
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350280"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VF097_10510"
     CDS             <1..811
                     /locus_tag="VF097_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010070205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEX6263265.1"
                     /translation="GSGIVEVCAKADCEVVTVEVDDQAMDRGRQRFEKSLGTAVDRGK
                     LEEGARDQILGRITSTTDLDAAADADLVIEAVTENLDEKLRVFRRLDEITRDEVILAT
                     NTSSLPIVKLAAATKRPDRVVGMHFFNPPPIMTLLEIVRALTTSDETLGFAKGMGERL
                     GKTTVLSKDRAGFIVNMLLIPYLNNAVRMLEEGFASREDIDTAIQLGLGHPMGPLTLL
                     DFIGLDTAVYVSEVLFEEFRDPDYSAPPLLRRMVDAGWHGRKTGKGFYDYD"
     gene            816..1655
                     /locus_tag="VF097_10515"
     CDS             816..1655
                     /locus_tag="VF097_10515"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6263266.1"
                     /translation="MPKAIDLHVHLPTDEWLDNALGPHLEATERYFRKSPPRKTLDEV
                     AEEYASLDVLGVVLDWDDESVTGRGWLGNEFMASLGERYPGVLMGFGSVDPHREDAVA
                     EADRVADLGLRGLKFHPTMQRFDPGDERYYPIYERMQERGLVALMHTGTCGIGAGTPG
                     AGGTKIRYSHPAFLDSVGADFPEVTWIAAHFGWPWYPECLAYALHKSNVWIELSGWAP
                     GYLPSEVRREAGRRLNDRTLFGSDYPFIPLDRWFSEFEDLGYEEDQKANILRRNAARL
                     LGL"
     gene            complement(1656..2984)
                     /locus_tag="VF097_10520"
     CDS             complement(1656..2984)
                     /locus_tag="VF097_10520"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEX6263267.1"
                     /translation="MVPKILAVLVAALALLPVPPARAEVHLMKIAEVFAGVAAAPQAQ
                     YVELQMHALGQNFTNGAWIEVFDSANASQGTFTFTENQANGNNQSKILIATAEAEIFF
                     GVDADLVMGSAAIPPAGGKVCFQAPGHGMIDCVAWGSYSGPSSGVGIPFRAGEGIPSG
                     AAMERSLKGNGTLEAGDDTNDSAVDFRFGLPAPRNNGGGAGGAPGGALSFHGNLLVPE
                     ADEVHLVPVQRLGLNEDPVTVNYLALGGSATDGLDYHAVEGTLQWPNGDNDDASFNVE
                     IIDDADPEGPETILMRIRSPVGGAVLGPIPDATITIEANDDTGPPTSRITKPKHRKRY
                     PAKKVRRLTGSASDPEVGIALVQIALQQRLGKGCRWWNGRKFVKRACSSPRWVGAKGG
                     ASWSYRLSKLLPSSTGTIKRYELRSRARDEAGNLEASFKRGRNRNRFEVR"
     gene            complement(3053..4153)
                     /locus_tag="VF097_10525"
     CDS             complement(3053..4153)
                     /locus_tag="VF097_10525"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX6263268.1"
                     /translation="MDLAEFRAQFPALADHTWLNTATAPPAARPVAEELRRVQREWET
                     GEFAWTDWEAEAYATRPLFARLIGADPDEVALVSSVSYAAATVAASLSPRRIVVGARE
                     FRSNYFPWVALADRGFEIVEVPANAEGVVTTDALLEGLAEGALVAVSEVQSSNGYRID
                     LARLTRETQAQGGRVFVDLCQSLGCLRFDAGALGVDYAAVHGYKWMLGPRGAAWLYVR
                     RDHVDECPPLTPSWKSPEDPYAEYYGGPLEYAKTARKLDVSLAWFSWPGARAALELLL
                     ALDAEAVEERAVALARAFREGAGERGYAVCTEELPSQIVGMEIPDAEGLQRRLREQRV
                     VAAVRGGFLRIGFHGFNDERDVERGLAALDRA"
     gene            complement(4156..5076)
                     /locus_tag="VF097_10530"
     CDS             complement(4156..5076)
                     /locus_tag="VF097_10530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6263269.1"
                     /translation="MKVSLVLTVQDAAETMERFLDSVRAQTLQPDEIVVVDRGSTDGT
                     LDILRAAGDVVLLEAPGASIARGRNLAIEVATHDLIAVSEADCVLSPRWLEQLAMALE
                     AGADVAMGYYRPIAHTLFETAAGAIAAPEPHEIDEVRFMPSSRSIAFRRDMFERTGGY
                     PEWLESGEDLYLDHRLRDLYADMRFVPEAVAHWRIRPTLGETFRQYFRYAEGDGRAGM
                     YPERHLLRFTAYGLLAYAAARRRWPLLAALALGGSFYAGRRVRRLTRMLPGRPAAQAA
                     AAALIPPMMAMIDAAKMAGYISGLLEKARS"
     gene            complement(5103..5408)
                     /locus_tag="VF097_10535"
     CDS             complement(5103..5408)
                     /locus_tag="VF097_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6263270.1"
                     /translation="MSARHTPNSGPPPSREDLMARFAEAGLRPRWWGNAAGDEYGSHT
                     HEHHKVLYCLEGSITFHTEEGDLELRPGDRLDLDAGTSHAATVGPNGVTCVEAYVEG"
     gene            complement(5405..5671)
                     /locus_tag="VF097_10540"
     CDS             complement(5405..5671)
                     /locus_tag="VF097_10540"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEX6263271.1"
                     /translation="MTRRGKPPGPPLRWMPVAQYTDMESARLMAGLLESEGIEVVLDP
                     PDVTSSWDGGMARVLGYRIKVMVLEHRQTEAAEIVQGVEEGRHR"
     gene            5728..6837
                     /locus_tag="VF097_10545"
     CDS             5728..6837
                     /locus_tag="VF097_10545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6263272.1"
                     /translation="MTPSRDVRTGFERRDLPLVVVSAVALVSGGPLTILRQAVAAARV
                     STDANFLFLVHDAGSWDEGPNVRFASFPGARRSYLWRVLTEYLRFPGLSRRWRPDVWL
                     SLHDVTPPVVARRQAVYCHNPLPYWRPTLRDLRLHPWEVVRSFLYGWVYRTNARRNDR
                     VIGQLPWFTEFIGRYMRVSRDRLLVVAPRTEAPVEIEEVPPPAGTGLACVYASLPRVF
                     KNFEEAIALCDQPGVRLTVTLSGDENRYARWVRERARGHVTFAGRMAHAEALAAMARA
                     DVVLFPSRLETFGLPVQEAIDLGRTLVLPVRPWTVAIAGEYPRAHFYRTVDEGRAILA
                     ALRDDREPLGVRPPVPESDLPRLSGFGELYHFLLR"
     gene            6861..>7773
                     /locus_tag="VF097_10550"
     CDS             6861..>7773
                     /locus_tag="VF097_10550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6263273.1"
                     /translation="MRSPAVAFDVTPLAGFRTGIGLAVQETHDALVDAGAELVPYAIG
                     LRVRTDGLPGGTRVVRLPTRGLVAAWGRAGRPRIDRWVRGARVVHATNYLTPPSRLPT
                     LVTIHDCAFALFPETVTRVVLGFARVLRRAVARGAWVHCATEAIAEEVEGLFGPGLRR
                     EGRIAVVPFGVPRLDASEPLAGELQERLRGRPYLLALGTIEPRKNLARLVRAFGRMAG
                     EHPDLRLVVAGPDGPGRPEVDDAIAGLDPDARGRVVLAGAVGPGQRRTLIGEAAVLAY
                     PSIYEGFGFPMLEAMTLGVPVVGGAAGA"
BASE COUNT         1034 a         2724 c         2847 g         1168 t
ORIGIN      
        1 ggggtcgggc atcgtcgagg tctgcgccaa ggccgactgc gaggtcgtga cggtggaggt
       61 cgacgaccag gcgatggacc ggggccgtca gcgcttcgag aagtcgctgg gcaccgcggt
      121 ggaccggggc aagctcgagg agggcgcccg cgaccagatc ctgggccgca tcacctcgac
      181 cacagacctg gacgcggcgg ccgacgccga cctggtgatc gaggccgtga ccgagaatct
      241 ggatgagaag ctccgcgtct tccgcaggct cgacgagatc acccgcgacg aggtgatcct
      301 ggccacgaac acctcctccc tccccatcgt gaagctggcg gcggccacga agcgtcccga
      361 ccgcgtcgtg gggatgcact tcttcaaccc gcccccgatc atgacgctgc tggagatcgt
      421 ccgggcgctc acgacctcgg acgagaccct cgggttcgcc aaggggatgg gtgagcggct
      481 ggggaagacg accgtcctct ccaaggaccg ggccggcttc atcgtcaaca tgctgctgat
      541 cccgtacctg aacaacgccg tgcggatgct ggaggagggc ttcgccagcc gcgaggacat
      601 cgacaccgcg atccagctcg gcctgggcca cccgatgggg ccgctcaccc tgctcgactt
      661 catcgggctg gacacggccg tgtacgtctc ggaggtgctg ttcgaggagt tccgcgaccc
      721 ggactactcg gcgcccccgc tgctccgccg catggtggac gcgggatggc acgggcggaa
      781 gacgggcaag ggcttctacg actacgactg agctcgtgcc gaaggcgatc gacctccacg
      841 tccacctgcc gaccgacgag tggctcgaca acgcgctcgg tccccatctc gaggcgaccg
      901 agcgctactt ccggaagtcc ccgccgcgta agaccctcga cgaggtggcc gaggagtacg
      961 cgtcgctcga tgtgctcggg gtggtcctgg actgggacga cgagtcggtc acgggccgcg
     1021 gatggctggg caacgagttc atggcctcgc tcggcgagcg gtacccgggc gtcctgatgg
     1081 ggttcggctc ggtagacccc caccgcgagg acgccgtcgc cgaggcggat cgcgtggccg
     1141 acctcggcct gaggggcctg aagtttcacc ccacgatgca gcggttcgac ccgggcgacg
     1201 agcgctacta tccgatctac gagcggatgc aggagcgcgg cctggtggcg ctgatgcaca
     1261 cggggacgtg cgggatcggg gcggggacgc ctggggccgg cggcacgaag atccggtatt
     1321 cgcatccagc gttcctcgac tccgtgggcg cggacttccc cgaggtgacc tggatcgccg
     1381 cgcacttcgg ctggccctgg tacccggagt gcctcgccta cgccctgcac aagtcgaacg
     1441 tgtggatcga gctctcgggg tgggcccccg gctacctccc gtccgaggtg cggcgcgagg
     1501 cgggacgccg cctcaacgac cgcaccctgt tcggcagcga ctaccccttc atcccgctcg
     1561 accgctggtt ctcggagttc gaggacctgg ggtacgagga ggatcagaag gcgaacatcc
     1621 tccgccgcaa cgcggcgcgc ctcctcggcc tctgactagc ggacctcgaa ccggttccgg
     1681 ttgcgccccc gcttgaacga ggcctcgagg ttgccggcct cgtcccgggc ccgggaccgc
     1741 agctcgtagc gtttgatcgt gccggtgctc gaggggagga gcttcgagag ccggtagctc
     1801 cacgaggcgc cccctttcgc cccgacccaa cggggggagg agcacgcgcg cttgacgaac
     1861 ttcctcccgt tccaccaccg acatcccttc ccgagacgct gctggagggc gatctggacg
     1921 agggcgatgc cgacctccgg gtccgacgcg ctgcccgtga gcctccggac cttcttcgcc
     1981 gggtagcgct tgcggtgctt gggcttcgtg atgcggctcg ttggcggccc cgtgtcgtcg
     2041 ttcgcctcga tcgtgatggt ggcgtcgggg atcgggccga gcaccgcccc gcccaccggg
     2101 ctgcggatgc gcatgaggat cgtctccggc ccctcgggat cggcgtcgtc gatgatctcg
     2161 acgttgaagg aggcgtcgtc gttgtcgcca ttgggccact ggagcgtgcc ctccaccgcg
     2221 tggtagtcga ggccgtcggt ggccgagccc cccagcgcga ggtagttcac ggtgacgggg
     2281 tcttcgttga gccccaggcg ctggacgggg acgaggtgga cctcgtcggc ctcggggacg
     2341 agcaggttcc cgtggaagga cagcgccccc cccggcgccc cccccgcgcc gcccccgttg
     2401 ttgcggggcg ccgggaggcc gaaccggaag tccacggcgc tgtcgttcgt gtcgtcgccc
     2461 gcctcgagcg tcccgttccc cttcaggctc cgctccatcg ccgctccgga agggatcccc
     2521 tcgcccgcgc ggaacgggat ccccacgccg ctgctcggac ccgagtagct cccccaggcg
     2581 acgcagtcga tcatgccgtg gcccggggcc tggaagcaga ccttgccgcc ggccgggggg
     2641 atcgccgcgg agcccatgac gaggtcggcg tccacgccga agaagatctc ggcctcggcg
     2701 gtggcgatca ggatcttgct ctggttgttc ccgttcgcct gattctcggt gaacgtgaag
     2761 gttccctgcg aggcgttggc gctgtcgaag acctcgatcc acgcgccgtt cgtgaagttc
     2821 tggccgagcg cgtgcatctg gagctccaca tactgcgcct gcggggcagc cgccacgccg
     2881 gcgaacacct ccgcgatctt catcaggtgg acctcggccc tggcgggagg gaccgggagc
     2941 agggccagcg cggccacgag cacggcgagg atcttcggga ccatgggctc cgaccttcct
     3001 tcgcttgtgg cggacgagcc taccccaagt cgccaagaag ggagcccgag cgtcaggccc
     3061 ggtcgagggc ggccagtccg cgctcgacgt cgcgctcgtc gttgaagccg tggaagccga
     3121 tccggaggaa gcccccgcgc acggcggcca ccactcgctg ctcgcggagg cgtcgctgga
     3181 gcccctcggc atcggggatc tccatcccca cgatctggga ggggagctcc tcggtgcaga
     3241 cggcgtagcc gcgctcgccc gcgccttctc ggaaggcgcg ggcgagcgcg accgcccgct
     3301 cctccacggc ctcggcgtcc agcgccagaa ggagctcgag cgccgcgcgc gccccgggcc
     3361 acgagaacca cgccagcgag acgtcgagct tccgggcggt cttcgcgtac tcgagcggcc
     3421 cgccgtagta ctcggcgtag gggtcctcgg gcgacttcca gctcggcgtc aggggagggc
     3481 actcgtcgac atggtcgcgt cgcacgtaga gccacgccgc cccccgcggg ccgagcatcc
     3541 acttgtagcc gtggaccgcc gcgtagtcga ccccgagcgc tcccgcgtcg aaccggagac
     3601 accccagcga ctgacagaga tcgacgaaga cgcggccccc ctgcgcctgg gtctcccggg
     3661 tgaggcgggc gaggtcgatc ctgtacccgt tcgaggactg gacctcgctg accgcgacca
     3721 gagccccctc ggccaggccc tcgaggagcg cgtcggtcgt gacgacgccc tcggcgttgg
     3781 cggggacctc gacgatctcg aacccgcggt cggccagcgc cacccagggg aagtagttgc
     3841 tgcggaactc ccgcgccccg accacgatcc tgcgtggcga gagcgaggcg gccacggtcg
     3901 ccgcggcgta ggacacggag gagacgaggg ccacctcgtc gggatcggcc ccgatgagcc
     3961 gggcgaagag cggacgcgtc gcgtacgcct ccgcctccca gtcggtccag gcgaactcgc
     4021 ccgtctccca ctcgcgctgg acgcggcgga gctcctcggc caccggccgg gccgccggag
     4081 gagcggtggc ggtgttgagc caggtgtggt cggccagcgc cgggaactgg gcccggaact
     4141 cggccaggtc caccctcagc tcctcgcctt ctccaggagg ccggagatgt agccggccat
     4201 cttcgctgcg tcgatcatgg ccatcatcgg cgggatcagg gccgcggcgg ccgcctgcgc
     4261 cgcgggccgt ccgggcagca tcctggtcag gcgccgcacc cgtctccccg cgtagaagga
     4321 gcctccgagc gcgagcgccg ccaggagcgg ccacctgcgc ctcgctgcgg cgtacgcgag
     4381 caggccgtat gcggtgaagc ggaggaggtg ccgctccggg tacatcccag cccggccgtc
     4441 cccctccgcg taccggaagt actggcggaa ggtctcgccc agcgtggggc ggatccgcca
     4501 gtgcgcgacg gcctcgggca cgaaccgcat gtcggcgtag aggtcgcgaa ggcggtggtc
     4561 gaggtagagg tcctcccccg actcgagcca ctccgggtac ccgcccgtcc gctcgaacat
     4621 gtcacgacgg aaggcgatcg accgggacga gggcatgaag cggacctcgt cgatctcgtg
     4681 cggctcgggc gcggcgatcg cgcccgccgc cgtctcgaac agggtgtgcg cgatcggacg
     4741 gtagtagccc atggccacgt cggccccggc ctcaagcgcc atcgccagct gctcgagcca
     4801 gcgaggcgag aggacgcagt cggcctcgga gacggcgatc aggtcgtggg ttgccacctc
     4861 gatggccagg ttgcgtcctc tggcgatgct cgctccgggc gcctcgagca ggacgacgtc
     4921 gccggcggcc cggaggatgt cgagggtgcc gtcggtcgag ccccggtcca cgaccacgat
     4981 ctcgtcgggc tggagggtct gcgcccggac cgagtcgagg aagcgctcca tcgtctcggc
     5041 cgcgtcctgg acggtgagga ccaggctgac cttcatgggg gagagtgtag acgaggctgg
     5101 ggtcacccct cgacgtaggc ctcgacgcac gtgacgccgt tcgggccgac cgtggcggcg
     5161 tgcgatgtgc cggcgtcgag gtcgagccgg tcgccgggcc ggagctcgag gtcgccttct
     5221 tccgtgtgga aggtgatcga gccctcgagg cagtacagga ccttgtggtg ctcgtgcgtg
     5281 tgcgacccgt actcgtcccc ggcggcgttg ccccaccagc gaggccgaag gcccgcctcc
     5341 gcgaagcggg ccatgaggtc ttcgcgggag ggcggcgggc cgctgttcgg ggtgtgcctc
     5401 gcgctcaccg gtggcggccc tcctcgaccc cctgcacgat ctcggccgcc tcggtctgcc
     5461 ggtgctcgag aaccatgacc ttgatccggt agccgaggac ccgcgccatc cccccatccc
     5521 aggagctcgt cacgtcgggc gggtcgagga cgacctcgat cccctccgat tcgaggaggc
     5581 cggccatgag ccgcgccgac tccatgtccg tgtactgggc aacgggcatc caccggagcg
     5641 gcgggcccgg cggcttccct cggcgtgtca cgccggagcc tcggggatgc ggagggcctc
     5701 gcggtcgtgt tggatggcct cccggagatg acgccgagcc gagacgtccg gacggggttc
     5761 gagcggcggg acctgccgct ggtcgtggta tcggcggtgg cgctcgtctc gggcgggccc
     5821 ctgacgatcc tccgccaggc ggtggccgcg gcccgggtct cgaccgatgc gaacttcctg
     5881 ttcctcgtcc acgatgccgg ctcctgggat gagggcccca acgtgcggtt cgcgtcgttc
     5941 ccgggggcgc gacgatccta cctctggagg gtcctgaccg agtacctgcg gttcccgggc
     6001 ctctcgcgcc ggtggcgccc cgatgtctgg ctctctctcc atgacgtcac ccccccggtc
     6061 gtggctcgtc ggcaggccgt ctactgtcac aacccgctcc cgtactggcg accgaccctt
     6121 cgcgacctcc gcctccatcc ctgggaggtc gtccgctcgt tcctctatgg atgggtgtac
     6181 cggacgaacg cccggcggaa cgaccgcgtg atcgggcagc tgccgtggtt caccgagttc
     6241 atcgggcggt acatgcgcgt gtcccgggat cggctcctgg tcgtggcccc ccggaccgag
     6301 gccccggtgg agatcgagga ggtcccgccg ccggcgggca cgggcttggc atgcgtctac
     6361 gcctcgctcc cgagggtgtt caagaacttc gaggaggcga tcgcgctctg cgaccagccc
     6421 ggggtccggc tcacggtcac gctctcgggg gacgagaacc gctacgcccg gtgggtgcgg
     6481 gagcgggcgc gtggccacgt gaccttcgcc ggccggatgg cgcacgcgga ggcgctggcc
     6541 gcgatggcgc gggccgatgt ggtgctcttc ccgtcgcggc tggagacgtt cgggctgccg
     6601 gtgcaggagg cgatcgacct ggggcggacg ctcgtgctcc cggtgcggcc ctggacggtg
     6661 gcgatcgccg gcgagtaccc gcgggcgcac ttctaccgga ccgtggacga gggtcgggcc
     6721 atcctcgccg ccctccgtga cgatcgggag ccgctcgggg tccggccgcc ggttccggag
     6781 agcgatctgc ctcggctctc cgggttcggg gagctgtatc acttcctgct tcgctgaggc
     6841 gatgtggtag gaatcggccc atgcgctcgc ccgccgtcgc gttcgacgtc acgccgctgg
     6901 ccggcttccg gaccggcatc gggctcgctg tgcaggagac gcacgacgcg ctggtcgatg
     6961 ccggagccga gctcgttccg tacgcgatcg gcctgcgggt gcggaccgac gggctgccgg
     7021 gcggcacccg cgtggtccgg ctgccgacgc gggggctcgt cgcggcctgg ggtcgcgccg
     7081 gccgtccgcg gatcgaccgg tgggtacggg gagcgcgggt ggtgcacgcc acgaactacc
     7141 tcacgccgcc gagccgcctg ccgacgctgg tgacgatcca cgactgcgcc ttcgccctct
     7201 tccccgagac cgtgaccagg gtggtgctgg gcttcgcccg ggtgctccgg cgggcggtgg
     7261 cccggggggc ctgggtccac tgcgcgaccg aggccatcgc ggaggaggtc gagggcctgt
     7321 tcgggccggg cctgcgacgc gaggggagga tcgcggtggt gccgttcggg gtcccgaggc
     7381 tcgacgcgtc cgagcccctt gccggggagc tgcaggagcg gctccgagga cgcccgtacc
     7441 tgctggcgct cgggacgatc gagccgagga agaacctggc caggctcgtg cgagcgttcg
     7501 ggcggatggc gggcgagcat ccggacctcc ggctcgtggt ggccgggccc gacggccccg
     7561 gccgtccgga ggtggacgac gcgatcgcgg gcctcgatcc ggatgcgagg ggccgggtgg
     7621 tgctggcggg ggccgtcggg cccggccaac gccgaacgct gatcggggag gccgccgtgc
     7681 tggcctatcc ctcgatctac gagggtttcg ggttcccgat gctcgaggcc atgacgctcg
     7741 gggtgccggt ggtgggcggc gccgccggcg ccc
//
LOCUS       DASUTZ010000247         3852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1127074, whole genome shotgun sequence.
ACCESSION   DASUTZ010000247 DASUTZ010000000
VERSION     DASUTZ010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127074"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /gene="thrB"
                     /locus_tag="VF097_10555"
     CDS             complement(<1..798)
                     /gene="thrB"
                     /locus_tag="VF097_10555"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX6263274.1"
                     /translation="MRLTVRVPATAANLGPGFDALALALGLHNEVVCETSAEPAVEVS
                     GEGAGELPEDASNLIFRSMAFVAREAGGSLPSLRLQCRNEIPLERGLGSSASAVVAGV
                     LVADRLLGSGLDPDRLLGLAVDVEGHPDNVAACLRGGGVIAYLSRHGWRAERLEPHPH
                     LAPVVLIPEGERVPTQDARRVLPLEVPLRDAAFNLGRSALAVRALTHDPALLHEALED
                     RLHQARRLELAPGARALFRDLRDAEIPACVAGAGPTILAFEGLGGPIP"
     gene            complement(803..1855)
                     /gene="thrC"
                     /locus_tag="VF097_10560"
     CDS             complement(803..1855)
                     /gene="thrC"
                     /locus_tag="VF097_10560"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX6263275.1"
                     /translation="MAAPWRGIVEEYRERLPVTDSTPIVTLGEGGTPLLRSPSLSEET
                     GCEVYLKYEGANPTGSFKDRGMTVAISRALEQEAEAVVCASTGNTSASAAAYAARAGM
                     TCGVLVPRGRVASGKLAQALVHGARILEIDGSFDRALTVVRELAEGSAVTLVNSVNPD
                     RIEGQKTLAFEVCDALGRAPDHHLLPVGNAGNITSHWLGYREYAADGLIGEPPAMLGF
                     QAAGAAPIVRGEPVANPMTIATAIRIGNPASWEGAEKAAAESGGAIEAVTDRQILAAY
                     RRLAREGIFCEMASAASVAGLLQVSALGRLERGSTVVCVLTGHGLKDPEWAAATAPRP
                     ALVEADPGAVAAALGL"
     gene            complement(1855..3135)
                     /locus_tag="VF097_10565"
     CDS             complement(1855..3135)
                     /locus_tag="VF097_10565"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX6263276.1"
                     /translation="MGVRTLTVGFLGCGTVGAATLRLLDEHAEDIAERAGCRIEVARV
                     GVRDPGRERDVPVPADRFTGDLRSVVDDPEVEALVEVMGGVDPALDLILRALGKGKHV
                     VTANKELLASSGRDLFAAAESAGVDLRFEAAVGGGIPLIRPLKESLAGERVARVTGIV
                     NGTTNFVLTRMHEDGMSFDEALELAIEQGFAEPDPTADVEGHDAAAKCAILASIAFNS
                     RVVADDVAREGVGGVSREDVTFAEQLGYVVKPLAVAELDGDDVAVRVHPAMLPEDHPL
                     AAVRESFNAVFVEGPNVGELMFYGRGAGGEPTAVAVVGDLVDVARSGGRPVGCTCYRE
                     RRILSIEESEGQFYLLLAVVDRPGVLARIAGVFGEQGVSIRSVWQEGTGEQALIVMIT
                     HRANEGALRRTVEGLRALDVVHEVRSVLRVEGEG"
     gene            complement(3126..>3852)
                     /locus_tag="VF097_10570"
     CDS             complement(3126..>3852)
                     /locus_tag="VF097_10570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6263277.1"
                     /translation="ARDATLPEDLRRGDLVAFASTGAYEYAMASTYNKVGRPAVVLVG
                     GDGEARPILRREELGDLARLDVGPPPTDRVPAPPGIEVRPARARDARGVMDLVAEVAA
                     EGRFIRTERVAPSAWPYRARLRRSWTSDGAEIVALEDGKVVGHLGISRESVPALRHVA
                     AIGMSVRRDHRSRGVGSSLMSEAIRWAREVGVEKLSLSVFPHNASAIALYRKFGFVEE
                     GVFRGHTRKSYGYEDEVVMARWV"
BASE COUNT          521 a         1502 c         1362 g          467 t
ORIGIN      
        1 ggggatgggg ccgcccagac cctcgaaggc gaggatcgtg gggccggccc cggccacgca
       61 cgcggggatc tcggcgtctc gcaggtcccg gaagagcgcc cgagcgccgg gcgcgagctc
      121 caggcggcga gcctggtgca gccggtcctc aagggcctcg tggagcagcg cagggtcgtg
      181 ggtcagcgcg cggacggcca gggccgaccg gccgaggttg aaggccgcgt cgcggagcgg
      241 gacctcgagt gggaggacgc ggcgggcgtc ctgggtcggg acccgctccc cctccgggat
      301 caggacgacg ggcgcgagat ggggatgggg ctcgaggcgc tcggcgcgcc agccgtgacg
      361 ggagaggtac gcgatcacgc cgcccccgcg gagacaggcc gcgacgttgt cggggtgtcc
      421 ctccacgtcc accgccaggc ccaggagccg gtcggggtcg aggcccgagc caaggagacg
      481 gtcggccacc agaaccccgg ccaccaccgc ggaggccgag gagcccaggc cccgctccag
      541 ggggatctcg ttgcggcact ggagccgaag cgacggaagc gagccgcccg cctcccgggc
      601 cacgaaggcc atggaccgga agatgaggtt ggaggcgtcc tcggggagct cgccggcgcc
      661 ttcgcccgag acctcgacgg cgggctccgc ggacgtctcg cagacgacct cgttgtggag
      721 gccgagggcc agggcgaggg cgtcgaagcc cgggccgagg ttcgcggcgg tggccgggac
      781 gcggacggtg aggcgcatcc ccctagagcc ccagggcggc ggcgaccgcg cccgggtcgg
      841 cctccacgag cgccgggcgg ggcgcggtcg ccgccgccca ctccgggtcc ttcagcccgt
      901 gcccggtcag cacgcacacg accgtgctcc ctcgctccag ccgacccagg gccgagacct
      961 gcagcagccc cgcgaccgac gccgccgagg ccatctcgca gaagatcccc tcgcgggcca
     1021 gccgccggta cgccgcgagg atctgccggt cggtcaccgc ctcgatcgcc ccgcccgact
     1081 cggcggcggc cttctcggcc ccctcccacg aggccgggtt cccgatccgg atcgccgtgg
     1141 cgatcgtcat cgggttcgcc accggctcgc cgcgcacgat cggggcggcg cccgccgcct
     1201 ggaacccaag catcgccggc ggctcgccga tcagcccgtc ggccgcgtac tcccggtagc
     1261 cgagccagtg gctcgtgatg ttgccggcgt tccccaccgg gaggaggtgg tggtcggggg
     1321 cgcgccccag cgcgtcgcac acctcgaacg ccagcgtctt ctgcccctcg atgcggtcgg
     1381 ggttcaccga gttgacgagc gtcaccgcgc tcccctcggc cagctcacgg accaccgtca
     1441 gcgcccggtc gaagctgccg tcgatctcca ggatccgcgc gccgtgcacc agagcctggg
     1501 ccagcttccc cgaggccacg cggccccggg gcacgagcac gccgcacgtc atccccgccc
     1561 gggccgcgta ggccgccgcc gaggcgctcg tgttgccggt ggaggcgcac accacggcct
     1621 cggcctcctg ctccagcgcc cgggagatcg ccactgtcat cccccggtcc ttgaaggacc
     1681 ccgtggggtt ggcgccctcg tacttcaggt agacctcgca gccggtctcc tcggagagcg
     1741 agggcgagcg gagcagcggc gtgccgcctt ctccgagcgt gacgatcggg gtggagtccg
     1801 tcaccggaag ccgttcccgg tactcctcca cgatgccccg ccacggcgcc gccatcagcc
     1861 ctcgccctcc acgcggagca cgctccgcac ctcgtggacg acgtcgagcg cgcgcagacc
     1921 ctcgacggtg cggcggagcg cgccctcgtt cgcccgatgg gtgatcatca cgatcagcgc
     1981 ctgctcgccg gtcccctcct gccacacgct gcggatcgag accccctgct ccccgaagac
     2041 cccggcgatc cgggccagca cccccgggcg gtcgacgacc gcgagcagga ggtagaactg
     2101 cccctcgctc tcctcgatcg acaggatccg ccgctcgcgg tagcacgtgc atcccacggg
     2161 cctgccgccg ctccgggcga cgtccacgag atcgcccacg acggccaccg cggtgggctc
     2221 gccgccagcg ccccgcccgt agaacatcag ctccccgacg ttgggcccct ccacgaagac
     2281 cgcgttgaag ctctcgcgaa cggcggccag cgggtggtcc tccggcagca tggcgggatg
     2341 cacgcgcacg gccacgtcgt cgccgtcgag ctcggccacc gccaacggct tcaccacgta
     2401 gccgagctgc tcggcgaagg tcacgtcctc ccggctcacc ccacccaccc cctcccgggc
     2461 cacgtcgtcc gcgaccacgc gcgagttgaa ggcgatcgag gcgaggatcg cgcacttcgc
     2521 ggcggcgtcg tgcccctcga cgtcggcggt gggatcaggc tcggcgaacc cttgctcgat
     2581 cgccagctcc agcgcctcgt cgaacgacat cccgtcctcg tgcatacggg tcagcacgaa
     2641 gttcgtcgtg ccgttgacga tgccggtgac gcgggcgacg cgctcgcccg ccagcgactc
     2701 cttcagcggg cggatcaggg ggatacctcc gcccacggcg gcctcgaagc gcaggtcgac
     2761 gcccgcgctc tcggccgcgg cgaagaggtc gcggccgctc gaggccagca gctccttgtt
     2821 cgccgtcacg acgtgcttgc ccttccccag cgcgcgcagg atcaggtcga gggcgggatc
     2881 caccccgccc atcacctcga ccagggcctc gacctcgggg tcgtccacca cggagcgcag
     2941 gtcgcccgtg aagcgatcgg cgggtacggg cacgtcgcgc tcgcgcccgg ggtcccgcac
     3001 gcccacgcgg gccacctcga tgcggcaccc cgcccgctcg gcgatgtcct cggcgtgctc
     3061 gtccaggagg cgcagggtcg cggcgcccac cgtgccgcag ccgaggaacc cgaccgtcag
     3121 ggtcctcaca cccatcgggc catcacgacc tcgtcctcgt agccgtacga cttcctggtg
     3181 tggcctcgga agacgccctc ctcgacgaac ccgaacttcc ggtagagcgc gatcgcggag
     3241 gcgttgtggg gaaagaccga gaggctcagc ttctccacgc ccacctcgcg ggcccagcgg
     3301 atcgcctcgg acatcaggga ggaccccacg ccgcggctcc gatggtcccg ccggaccgac
     3361 atcccgatgg cggccacgtg gcggagggcg ggcacggact cccgggagat cccgaggtgc
     3421 cccacgacct tgccgtcctc cagcgccacg atctcggcgc cgtcggacgt ccacgaccgg
     3481 cggagccggg cgcggtaggg ccaggcggac ggggccaccc gctccgtccg gatgaagcgt
     3541 ccctcggccg cgacctcggc cacgaggtcc atcacgccgc gagcgtcccg ggcccgggcc
     3601 gggcggacct cgatccccgg gggggccgga acgcggtcgg tggggggcgg ccccacgtcg
     3661 agtcgggcca ggtccccgag ctcctccctc cggaggatcg ggcgggcctc cccgtcgccg
     3721 cccaccagca ccaccgcggg gcgccccacc ttgttgtagg tgctggccat cgcgtactcg
     3781 taggcccccg tggaggcgaa ggccacgagg tcgccgcgcc tcaggtcctc ggggagggtg
     3841 gcgtcgcgtg cg
//
LOCUS       DASUTZ010000248        13854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1127783, whole genome shotgun sequence.
ACCESSION   DASUTZ010000248 DASUTZ010000000
VERSION     DASUTZ010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13854
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127783"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..1310)
                     /locus_tag="VF097_10575"
     CDS             complement(42..1310)
                     /locus_tag="VF097_10575"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6263278.1"
                     /translation="MTDTTTARRTIGGAARVGLLGSERSMIAVRWLAAVFALVQILAY
                     KDAPYPEEIPVFALGLTAVGVLAAGNGALWIASHRAVSARALAQAGLALDVLVAEAIV
                     WLWAFDPGSALWAVLYVLPLEGAIKFQLRGALGTWAVVTVLYVAREFWGYATFEGFNL
                     QWNSITFRMGIGLLIALVGGLMARNLTRERALAEDRLARLRRIDRLRAGLVSTLAHDV
                     RNPIAAIRGTITILLSPRVGQLSKETRLDLLRSADRQAERLERLAIDLLDLARLERGR
                     LELTFESVSMAPLVGRALAFIEGGHQVEVEVPDDVVLRADPGRVEQILVNLVSNALRY
                     GREPFELRVSSSDHEVVVVVTDRGEGIPPDQQPTLFDPFRSEPDGGSVGFGLAIVRAL
                     AEAHGGSAMYRDRDGGGACFVVRLPVAGPG"
     gene            1414..2967
                     /locus_tag="VF097_10580"
     CDS             1414..2967
                     /locus_tag="VF097_10580"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX6263279.1"
                     /translation="MTLMQGRHAAERSPTHRLGFTGLVVLLLAGTAAVASWRFGIFSG
                     ASPEPSARDGDPPSAPTDPGEASPEPSPSPSSTPEPINTGIEGLTTFRGNATRTYHGR
                     GPVPEDPEILWRYPTSGGLCGQSSDAAGTRLWCGTGWTGQPNVVEWEGRIQIRFGAYD
                     YAVHVVGGRNGRALMEPFRTGDLIKGTVTSDPDGYPLLYVGSRDNQLRILALDRGRTM
                     EELWSLSADSAPDPTWNDDWDGSPLVIDDYLLEGGENSWFYVVKLNRGYDGQGRVTVN
                     PRIRVMVPGYDDELIAALGDEQVSIESSVAFHDGVAYFANSGGLVQGWDVSDVLLGGD
                     RHRRVFRFWTGDDTDASVVIDEEGYLYVASELERLNGRSREVGQLMKLDPRKPKDPLV
                     WSVPVPGEGGAGGLWATPAVHGRLVIAPTNTGRILAVNRNTGRIRWELRLPPPTWTSP
                     VIVDDVLILGDCAGVLHAYDLSRPGREPPELWSVQLEGCIESTPAVWDGMVYVGARGG
                     AVYGIGDRG"
     gene            2970..3422
                     /locus_tag="VF097_10585"
     CDS             2970..3422
                     /locus_tag="VF097_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX6263280.1"
                     /translation="MATDPLRPGDEVPGIPEVDLSPPALVAFYKVTCPTCQMAAPPME
                     RLHRTADGGFVAVVQDAHDRAAAFAREYGVSFASIPDEDPYPISRAWGVRVVPTLFAT
                     SEGRVEDLAESWDREGWARVAARFGELRGILVPAPSEEGDGLPPFRPG"
     gene            3472..4575
                     /locus_tag="VF097_10590"
     CDS             3472..4575
                     /locus_tag="VF097_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015289190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEX6263281.1"
                     /translation="MEFEAGLSTGERARPAAVEAAMAARAPLRDRPTLALVFSTPHHD
                     PAEVLDAVREAASPEAVLGCVGQAVVAGPREVEHGPAVAVWLARLPDRVEPYTVTFDG
                     GRFVGMPEGSGTHIVIADPFTFPADAFLDDLEERRPGTAVIGGMASGVYPPGSTRLFM
                     NDEVVTTGAVGARLPGGVAVRALVSQGCRPVGPPFTVTRAEGNLIAELGGRSPLDRLR
                     EIYAAADERDRGLLAGGLHLGRVVDEHAEEGDFLIRGVLGADRDTGAMAVGDQVRVGE
                     TVRFHVRDADSADEDLRRALEGLDRPRGALLFTCNGRGRRLFAGPNHDAALVAEELGG
                     PPVAGFFAAGELGPIGGRSFLHGFTASLALFYG"
     gene            complement(4579..5022)
                     /locus_tag="VF097_10595"
     CDS             complement(4579..5022)
                     /locus_tag="VF097_10595"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX6263282.1"
                     /translation="MSAPSDLHPTVAQELRQGGQRYTPVRRRLVGILVEADGPLTIPE
                     ILRRSPRLAQSSVYRNLAVLERAGVVHRVVTSEEHARYEVAEELSGHHHHHLICASCG
                     RVEDFVTPPRVERALARTMSEVAEGAGFSPTHHRLDLVGLCDRCS"
     gene            complement(5019..5897)
                     /locus_tag="VF097_10600"
     CDS             complement(5019..5897)
                     /locus_tag="VF097_10600"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX6263283.1"
                     /translation="MSLPWPFEREYMQLALAAGLVVGACAPLIGAFLVQKRMSLMGDG
                     LGHLAFAGVAGAILLGVWPLWGALLFTVVGALAVEGLRARGRTSGDLALAIAFYVGIA
                     SGVVLLGLAGSLNATVFSYLFGQILTVTSGEVAVVAGLGAGIVLAFALSRRALFATVS
                     DEEWARVAGLPVGALNALLAVLTAIIVVAAMRVVGLLLVAALMVLPVAGAQLLARSFG
                     GTLLWSSGIGVGSVLAGLVAARTLGLAPSGTIVLLAAAAFLAAALVSWVRGRLGGTSE
                     TTSLHFEERVAGGEDA"
     gene            complement(5894..6652)
                     /locus_tag="VF097_10605"
     CDS             complement(5894..6652)
                     /locus_tag="VF097_10605"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEX6263284.1"
                     /translation="MSRVSSDELARLEGVSFSFDGSPILSDVDLRVGRGEFVALVGPN
                     GSGKSTLLRILLGLLPAKGTVRLLGKTPDELRERWRIGYVPQRPTLAGDQVASVEEVV
                     AAGRLARRGWWRRLRREDRDVIGHAVESVGLLPLRHRRVSHLSVGQQQRVFIARAFAS
                     EPELLVLDEPISGVDAESQRRFRDSLLHTIEDHGTGVLLVSHELGAVSEDLDRVVVLK
                     GRVLFDGPPSDLMARGVSLGVHPEDLPFWLEELR"
     gene            complement(6640..7503)
                     /locus_tag="VF097_10610"
     CDS             complement(6640..7503)
                     /locus_tag="VF097_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012947761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEX6263285.1"
                     /translation="MTISAALLASGCGTEAADGVGGVVIVAGFFPLAEAAEIAGGDRV
                     HVENLTPAGSDPHDVELATDEVDTILDAELLLYLGGGFQPGVEELAGRRDDISIDLLR
                     DVDTLSEGGEVDPHVWLDPIRYASVVERIGRALAEVDREGADAYLANAEAHAEEIRAL
                     DARFERRLAACEHDLLVVSHAAFGYLADRYGLRQEAVSGISPESEPDPQRLAELIDLV
                     RAEGVTTVFTETLASPEVARTLAREAGVRTALLNALEGLTPEEEAAGETYLSVMERNL
                     ETIAEGLGCPE"
     gene            7618..10254
                     /locus_tag="VF097_10615"
     CDS             7618..10254
                     /locus_tag="VF097_10615"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6263286.1"
                     /translation="MSELPTGTITFLLTDIEGSTRILARLRDRYPALLEEHQRLLREV
                     FSAAGGVEVGTEGDSFFVVFPAAPNAVAAAAKAQQALESHEWPGGERVRVRMGLHTGE
                     AILGGDNYTGIDLHRAARIAAAAHGGQVLLSDATHALVAQTLPEGVAVRDLGEHRLKD
                     LPQPEHLHQLQIPGLPEDFPPIRSLETRRGNLPQPVTSFVGREGELAEIPEILAQTRL
                     LTLTGPGGSGKTRLSIQVGSELQDAFEDGVFFVPLASIEEPDLVVPTIAEVLRVEENP
                     GRPAIDTLIEHLADREILLVLDNFEQVVPAAGAVGEILAATEGVKVLATSRERLHLSG
                     EREYPVPPLRLPDLAALPPLEALSQFEAVQLFIQRASAVQPGFEVTNENAPAVAEICA
                     RLDGLPLAIELAAARVKLLPPDELLKRLGDSLALLSGGARDLPERQQTLRGAIAWSYD
                     LLDERERSFFRRLSVFMGGFTLEAAEEVCGGARELGDTLDLVASMLDKSLVRTFTGPA
                     GSRYMMLQTIGEYARELLEDSGEAGEVRARHARFVERILEDAVPKLFGPDRALWLDRL
                     AAEHDNLRAALTWALEAGDLEAGLRLGGSSWRFWQMRGHLREGREWLGRLLAHPTAVD
                     HPGPRADALEGLGGILYWMGDEEAAEVYEECLRLRRELGDAAAIGEALYNRASLNVFG
                     IGGAQDSQAALPLLEEALGIFRELGEGVGVAKVQWAVGSAHLHDGDYDAAESALSESI
                     DLYREAQDLFGEAWGLHMLAVTLLHEGRGEEALPHLRRAVDIFDEAGDRSAIPVLLFD
                     SAIYAYQHGDRDRALFLMSVAQKVQQETGVGLGEASVEFVGLLRPLMETMSDEEGERA
                     AAEARDITEEQAVEMIRELCSG"
     gene            10296..11399
                     /gene="hppD"
                     /locus_tag="VF097_10620"
     CDS             10296..11399
                     /gene="hppD"
                     /locus_tag="VF097_10620"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002694277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEX6263287.1"
                     /translation="MAQQEDFLPLMGIDHVEYYVGNARQAAYYYSTAFGFKVTAYRGP
                     ETKHRDAVSWLCEQGDVRFVFTGAQGPEHPIARHVLEHGDGVKDIALRVPDATSAWEE
                     TTKRGAESAMEPQVFEDEHGKVVRSAIKAYGETLHSFIERENYSGPFLPAFRQEEPYP
                     DDGPEVGIKAIDHCVANVELGKMNEWVRFYAEVMGFSQLMHFRDDQISTEYTALMSKV
                     MWDGSGRVKFPINEPAEGKKKSQIEEYLDYYRSPGVQHIAMQTEDIVQTVTALRERGV
                     RFIRVPETYYQDLAERVGEIDEDYSDLQNLAILADRDEEGYLLQIFTKIQQDRPTVFY
                     EFIERHGSRGFGLGNFKELFIAIEREQAERGNL"
     gene            11495..12013
                     /locus_tag="VF097_10625"
     CDS             11495..12013
                     /locus_tag="VF097_10625"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6263288.1"
                     /translation="MRILVRHVMTHDVATVSPSAGCQEIAQLMVDRHVRTVPVVEADG
                     TLVGLVTEAGLLEHVGGAREPLHTVELLSTPGRHPHPLPCELHATDVMTRGVVTSRRD
                     ATVPEAIDALVAARVRGLPVVDGNGVLLGIVDRGDLLRGLAPMIRSVDGVVGLHPAHG
                     RRRARREGLRAS"
     gene            complement(12015..12608)
                     /locus_tag="VF097_10630"
     CDS             complement(12015..12608)
                     /locus_tag="VF097_10630"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX6263289.1"
                     /translation="MAQRRRMRAPERRQQLLDVARKVFGRNGFHTVTMDAVAKEAGVT
                     KPILYDHFSSKRDLYLALLEADLASLRERVRTALETSRGNRERIRASFQVYFDFVDEH
                     AEGFRLLMTEAVGSERDFREMVERFRDDVHAEVTEVIHRESRGRVPMEDARDVALGLI
                     GMAETAAQANPGASKEDRRRTVDALVRLAWRGITGLA"
     gene            12697..>13854
                     /locus_tag="VF097_10635"
     CDS             12697..>13854
                     /locus_tag="VF097_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6263290.1"
                     /translation="MEFRLNEDQLTAQKWAREFAEKEIRPVAPEYDESEEFPWPVLKK
                     ATEVGLNSLEFYVEMAGADPTGLIMPIVLEESCWGCAGISLGIFGTGLPLATLAGTGT
                     PDQFLEWAPRMIGTPDHPKVGAFAVTESGAGSDVSALRTTAKRDGDEWVLNGQKIFIT
                     NGGIADVHIVVATVDPELGHRGQAAFIVGPDTPGISQGKKERKLGIRASHTAEVLLDD
                     CRVPLENVVGGMERLEAKLARGREKGGEPSRSSQALKTFELTRPLVAAQALGIARAAL
                     EYSVDYAKERTTFGRPIIEHQGIGFKLADMAMEVDAARLLTWRAAWMVRNDQLQRAEG
                     SMSKLKASEVAVWVTEQAIQVLGGYGYIKDFPVEKWYRDAKIYTLFEGTSEI"
BASE COUNT         2071 a         4883 c         4959 g         1941 t
ORIGIN      
        1 cgcgcgcgcc gcgcgatcga ccgcatgctc gagttcttgc cctagcccgg gccggcgacc
       61 ggcagccgca ccacgaagca ggcgcctcct ccatcgcggt cccgatacat ggccgagccg
      121 ccgtgggcct cggccagcgc ccgcacgatg gcgaggccga agcccacgga cccgccgtcg
      181 ggctccgacc ggaagggatc gaacagcgtc ggctgctggt ccggcgggat gccttccccc
      241 cggtccgtga cgacgacgac gacctcgtgg tccgaggacg agacgcgcag ctcgaacggc
      301 tcccgtccgt agcggagggc gttggacacg aggttgacga ggatctgctc caccctcccg
      361 gggtcggccc ggaggaccac gtcgtctggg acctcgacct cgacctgatg ccccccctcg
      421 atgaaggcga gagcgcggcc cacgagcggc gccatcgaca cgctctcgaa cgtcagctcc
      481 agccggcccc gctcgagacg agccaggtcg aggaggtcga tcgcgagccg ctccagcctc
      541 tcggcctgcc ggtcggcgct cctcagcagg tcgaggcggg tctccttcga cagctgcccc
      601 acccgggggg agaggagaat cgtgatcgtt ccccgtatcg ctgcgatcgg gttccgcacg
      661 tcgtgagcga gcgtggagac caggcccgcc cgcagccggt cgatcctgcg gagccgggcc
      721 aggcgatcct cggccagggc ccgctcgcgc gtgaggttcc gcgccatcag gcctcccacg
      781 agggcgatca ggaggccgat gcccatccgg aacgtgatgc tgttccactg gaggttgaag
      841 ccttcgaagg tcgcgtatcc ccagaactcg cgagccacgt agagcaccgt gacgaccgcc
      901 caggtcccga gcgcgcctcg gagctggaac ttgatcgcgc cctccagggg caggacgtag
      961 agcaccgccc acagggcgga gcccggatcg aacgcccaga gccagacgat cgcctcggcc
     1021 acgaggacgt ccagcgccag cccggcctgc gccagcgcgc gagcgctcac ggcgcggtgg
     1081 gatgcgatcc agagcgcccc gttgcccgcc gccaggacgc ctaccgccgt caggccgagg
     1141 gcgaagacgg ggatctcctc ggggtacgga gcgtccttgt acgccaggat ctggacgagg
     1201 gcgaagaccg ccgccagcca gcgaaccgcg atcatcgagc gctcggagcc cagcaggccc
     1261 acgcgcgcgg ccccgccgat cgtccggcgg gccgtcgtag tgtcggtcat gtgctcatcg
     1321 cgctccgccg cccctcgggg gccgcgacta gtgtcgcccg aaccgctctc ctcagtccat
     1381 gccttcttcc gagccggaga tccttcccgt agcatgacgc tgatgcaggg gaggcatgcc
     1441 gctgagcgtt cgcccaccca ccgtctcggg ttcaccggac tcgtcgtcct cctgctcgcc
     1501 ggcaccgccg cggtcgcctc atggcggttc gggatcttct cgggagcctc tccggagccc
     1561 tccgctcggg acggcgatcc cccttcggcc ccgaccgatc ccggtgaggc ctcccccgag
     1621 ccgagcccaa gcccctcctc cacccccgag cccatcaaca ccggcatcga ggggctgacg
     1681 accttccggg gcaacgccac ccgcacctac cacggccggg ggcccgtccc cgaggacccg
     1741 gagatcctgt ggaggtatcc gacctcgggc gggctctgcg ggcagtcctc ggacgcggcc
     1801 gggacgaggc tctggtgcgg caccgggtgg acggggcagc ccaacgtcgt ggagtgggag
     1861 ggccgcatcc agatccggtt cggggcctac gactacgcgg tccacgtcgt gggcggcagg
     1921 aacggccgtg cgctgatgga gcccttcagg acgggggacc tgatcaaggg gacggtcacc
     1981 agcgaccccg acgggtaccc gctgctctac gtgggctccc gtgacaacca gctccggatc
     2041 ctcgcgctgg atcgagggcg caccatggag gagctctgga gcctctcggc ggattcggcg
     2101 ccggatccga cgtggaacga cgactgggac ggctccccgc tcgtgatcga cgactacctc
     2161 ctcgagggag gggagaactc ctggttctac gtcgtgaagc tcaaccgagg ctacgacggc
     2221 caggggaggg tcaccgtgaa ccccaggatc cgcgtgatgg tccccggcta cgacgacgag
     2281 ctaatcgcgg ccctgggtga cgagcaggtc tcgatcgaga gctcggtcgc gttccacgac
     2341 ggcgtggcgt acttcgccaa ctcggggggg ctggtgcagg gctgggacgt ctccgacgtc
     2401 ctcttggggg gcgatcggca ccgccgcgtc ttccgcttct ggacgggcga cgacaccgac
     2461 gcctcggtcg tgatcgacga ggaggggtac ctctacgtgg cctccgagct cgagcggttg
     2521 aacggccgct cccgagaggt ggggcagctc atgaagctcg atccccgcaa gccgaaggac
     2581 ccgctcgtat ggtcggttcc cgtccctgga gagggaggcg cgggaggcct ctgggccacg
     2641 cctgccgtgc acgggcggct cgtgatcgcc cccaccaaca cgggacgcat cctggccgtg
     2701 aaccgcaaca cgggacgcat ccgctgggag ctgcggctcc cgccgcccac gtggacctcg
     2761 ccggtgatcg tcgacgacgt gctcatcctc ggcgactgcg cgggggtgct ccacgcgtac
     2821 gacctctccc gccccgggcg cgagcccccc gagctctgga gcgtgcagct cgaggggtgc
     2881 atcgagtcga ccccagccgt gtgggacggc atggtctacg tgggggccag gggcggcgcg
     2941 gtctacggga tcggggatcg ggggtagcgg tggcgacgga tccgctccgt ccgggcgatg
     3001 aggtccccgg gatccccgag gtcgatctct cgcctcccgc gctcgtcgcc ttctacaagg
     3061 tcacctgccc gacgtgccag atggcggctc ccccgatgga gcggctccac cgaacggctg
     3121 acgggggctt cgtggccgtc gtgcaggacg cccatgaccg cgccgccgcg ttcgcgaggg
     3181 agtacggggt ctcgttcgca tcgatcccgg acgaggaccc ctatccgatc tcgcgtgcct
     3241 ggggtgtacg ggtcgtcccc acgctgttcg ccacgtcgga gggacgcgtg gaggacctcg
     3301 ccgagtcctg ggatcgagag gggtgggccc gggtcgcggc gcgcttcggg gagctccgcg
     3361 ggatcctggt gcccgccccc tccgaggagg gcgacgggct tcctcccttc cgacccgggt
     3421 gaacgtccag gagcgcccgc tcggtctgag cgggccggat agggtggggg catggagttc
     3481 gaggcgggcc tctcgacggg ggagcgcgcg cggcctgcgg ccgtcgaggc cgcgatggcc
     3541 gcgcgcgctc ccctccgcga tcgccccacg ctggcgctcg tcttctccac gccccatcac
     3601 gatcccgccg aggtgctcga cgcggtccgg gaggcggcgt ccccggaggc cgtcctcggg
     3661 tgtgtgggtc aggccgtcgt ggcgggcccg cgggaggtcg agcacggacc cgcggtggcc
     3721 gtctggctgg cccgcctccc ggaccgcgtc gagccgtaca cggtgacctt cgacggcggt
     3781 cgcttcgtcg ggatgccgga gggctccgga acccacatcg tgatcgccga ccccttcacg
     3841 ttcccggccg acgcgttcct cgacgacctg gaggagcggc gccccggcac cgcggtgatc
     3901 ggcgggatgg ccagcggcgt ctaccctccg ggctccactc ggctcttcat gaacgacgag
     3961 gtcgtgacga ccggcgccgt cggggccagg ctcccgggcg gcgtggcggt ccgcgcgctc
     4021 gtctcccagg ggtgccgtcc ggtgggcccg ccattcaccg tcacgagggc cgaggggaac
     4081 ctgatcgcgg agctcggggg ccgctcgccc ctcgaccggc tccgcgagat ctacgccgcg
     4141 gccgacgagc gggatcgcgg tctcctggcc ggcggactgc acctcggccg cgtggtggac
     4201 gagcacgccg aggaggggga tttcctgatc cgaggcgtgc tgggggccga ccgcgacaca
     4261 ggcgcgatgg cggtgggcga ccaggtgcgc gtcggggaga cggtgcggtt ccacgtgcgc
     4321 gacgccgact cggccgatga ggatctccgc agggccctgg aggggctcga ccgtccgcgc
     4381 ggcgccctcc tgttcacctg caacggtcgg gggcggaggt tgttcgccgg gcccaaccac
     4441 gacgcggcgc tggtggccga ggagctcggg gggccgccgg tggcgggctt cttcgccgcg
     4501 ggggagctgg gaccgatcgg gggccggagc ttcctccacg gcttcacggc gagcctggcg
     4561 ctcttctacg ggtagcgctc agctgcagcg gtcgcagagg cccacgagat cgagccggtg
     4621 gtgcgtcgga gagaacccgg ctccctcggc cacctcggac atcgtccggg cgagcgctcg
     4681 ctccactcgg ggcggcgtga cgaagtcctc gacgcgcccg cacgaggcgc agatcaggtg
     4741 gtggtggtgg tggccggaga gctcctcggc gacctcgtac cgcgcgtgct cctcgctcgt
     4801 aacgacccgg tgcaccaccc cggcccgctc cagcacggcg aggttcctgt acacggagct
     4861 ctgggcgagc ctcgggctcc ggcgaaggat ctcggggatc gtgaggggtc cgtccgcctc
     4921 gacgaggatc cccacgagac gccggcgcac gggggtgtag cgctggccgc cctgacggag
     4981 ctcctgcgcc acggtcgggt ggagatcgga cggggcgctc acgcgtcctc ccctcccgcc
     5041 acgcgctcct cgaagtggag ggaggtggtc tcgctggttc ctccgagccg acctcggacc
     5101 catgagacga gcgccgcggc gaggaaggcc gccgcggcga ggaggacgat cgtcccgctc
     5161 ggggccagcc ccagcgtcct cgccgcgacc agccccgcga gcaccgatcc caccccgatc
     5221 cccgacgacc agaggagcgt ccccccgaac gagcgggcga gcagctgggc acccgccacg
     5281 gggaggacca tcagcgccgc gacgagcagg agccccacca cgcgcatcgc ggccacgacg
     5341 atgatcgcgg tcaggacggc gaggagcgcg ttgagtgcgc cgaccgggag gcccgccacc
     5401 cgcgcccact cctcatcgct gaccgtggcg aagagggcgc gcctggagag cgcgaaggcc
     5461 aggacgatgc ccgcccccag gcccgccacg acggcgacct cgccgctcgt gacggtcagg
     5521 atctggccga acaggtagct gaagacggtc gcgttcagcg agcccgctag gccgagcagg
     5581 accacgccgg acgcgatccc cacgtagaag gcgatcgcca gcgccaggtc ccccgacgtc
     5641 ctcccccggg cgcggagccc ctccacggcg agggcgccca ccaccgtgaa cagcagcgcc
     5701 ccccacaggg gccacacgcc gaggaggatg gcgccggcca cgcccgcgaa cgcgagatga
     5761 cccaggccat cccccatcag cgacatccgc ttctggacaa ggaacgcgcc gatcagcggc
     5821 gcgcaggcgc ccacgactag gccggcggcc agcgcgagct gcatgtactc ccgctcgaag
     5881 ggccacggaa ggctcaccgc agctcctcca gccagaaggg gaggtcctcg gggtgcaccc
     5941 cgaggctcac cccccgggcc atcaggtcgg agggcgggcc atcgaagagg acgcggccct
     6001 ttagcacgac gacgcgatcc aggtcctcgg acaccgcccc cagctcgtgg gagacgagga
     6061 gcacccccgt cccgtggtcc tcgatcgtgt ggaggagcga gtcgcggaag cgtcgctggg
     6121 actcggcgtc cacgcccgag atcggctcgt cgagcacgag cagctccggc tcggaggcga
     6181 acgccctggc gatgaacact cgctgctgct gtcccaccga caggtgactg acgcgtcggt
     6241 ggcgcagcgg cagcaggccc accgactcga ccgcgtgtcc gatcacgtcc cggtcctccc
     6301 tccgcaggcg gcgccaccac ccgcggcggg cgagccggcc ggccgcgacg acctcctcga
     6361 cgctggccac ctgatctccc gcgagcgtcg gccgctgggg cacgtagccg atccgccagc
     6421 gctcgcggag ctcgtccggc gtcttcccca gcagtcggac cgtccccttt gcggggagca
     6481 ggccgagcag gatgcgcagc agggtcgact tgccgctccc gttgggtccc acgagcgcga
     6541 cgaactcccc ccgcccgacc cggaggtcca cgtcagagag gatgggggag ccgtcgaacg
     6601 agaaggagac cccttccagc cgcgccagct cgtccgagct cactcgggac atccgagacc
     6661 ctccgcgatc gtctccaggt tgcgctccat caccgagagg taggtctcgc cggcggcctc
     6721 ctcctccggc gtcaggccct cgagcgcgtt caggagggcg gtccggaccc cggcctcccg
     6781 ggccaacgtc cgtgcgacct ccggtgacgc cagcgtctcg gtgaagaccg tcgtgacccc
     6841 ttcggcgcga acgagatcga tcagctccgc cagccgctga ggatcgggct cgctctccgg
     6901 cgagattccc gagacggcct cctgccgcag gccgtaccgg tccgcgaggt agccgaaggc
     6961 ggcgtgcgag acgaccagga ggtcgtgctc gcacgccgcc aggcgccgct cgaagcgcgc
     7021 atcgagcgcg cggatctcct cggcgtgagc ctccgcgttg gccaggtagg cgtccgcccc
     7081 ctcgcggtcg acctcggcca gcgcgcggcc gatccgctcc accaccgagg cgtaccggat
     7141 cggatcgagc cacacgtggg ggtcgacctc tcccccttcg gagagcgtgt ccacgtcccg
     7201 gaggagatcg atggagatgt cgtccctgcg ccctgcgagc tcctccaccc ccggctggaa
     7261 gccacccccc aggtacagga ggagctcggc gtcgagtatc gtgtcgacct catcggtggc
     7321 cagctccacg tcgtgggggt cgcttccggc gggggtgagg ttctcgacgt ggacgcgatc
     7381 gccgcccgcg atctcggccg cctcggcgag agggaagaag cctgccacga tgacgactcc
     7441 accgacgccg tcggccgcct cggttccgca tccggaggcg agcagcgccg cgctgatggt
     7501 catgagaatc atcctcatta tcagggggac tgtagcaggg tcttcccttc cgccgcaacc
     7561 ctctgtgagc ggcacgagcg cgggcgcata atggaccgcg tcgatcgagg gaggaggatg
     7621 agcgagcttc cgaccgggac gatcacgttc ctgcttacgg acatcgaggg gagcacccgg
     7681 atcctggccc gcctcaggga ccgctacccc gcgctgcttg aggagcacca gcgcctcctc
     7741 cgggaggtct tctcggcggc cggaggggtg gaggtgggca cggagggcga ctcgttcttc
     7801 gtcgtgttcc cggccgcgcc caacgcggtg gccgccgccg ccaaggccca gcaggccctc
     7861 gagagccacg agtggccggg aggggagcgg gtccgcgtgc ggatggggct gcacaccgga
     7921 gaggccatcc tgggcggcga caactacacg ggcatcgacc tgcaccgggc ggcgcgcatc
     7981 gcggccgccg cgcacggcgg ccaggtgctc ctctcggacg ccacgcacgc gctcgtggcc
     8041 cagactctcc ccgagggcgt ggccgtccgc gacctcggcg agcaccggct gaaggacctg
     8101 ccccagccgg agcacctcca ccagctccag atccccgggc tgcccgagga cttccctccg
     8161 atccgctcgc tcgagacgcg ccgggggaac ctcccccagc ccgtcacgag cttcgtgggc
     8221 cgcgaggggg agctcgccga gatcccggag atcctggcac agacccggct cctcaccctg
     8281 accgggccgg gcggctcggg caagacccgc ctctcgatcc aggtgggatc ggagctccag
     8341 gacgccttcg aggacggcgt gttcttcgtg ccgctggcat cgatcgagga gcccgatctc
     8401 gtggtcccca ctatcgccga ggtgctgcgc gtggaggaga accccggccg gcccgcgatc
     8461 gacacgctga tcgagcacct cgccgatcgc gagatcctgc tcgtgctcga caacttcgag
     8521 caggtcgtgc cggccgcggg agcggtgggg gagatcctgg ccgcgaccga gggggtgaag
     8581 gtcctggcga cgagccggga gcgcctccac ctctcggggg agcgcgagta ccctgtcccg
     8641 ccgctgcggc tccccgacct cgcggccctc ccgccgctcg aggcgctgtc gcagttcgag
     8701 gccgtccagc tcttcatcca gcgggcctcg gcggtgcagc cggggttcga ggtgacgaac
     8761 gagaacgcgc cggcggtcgc cgagatctgc gcgcgcctcg acgggctccc ccttgccatc
     8821 gagctcgcgg ccgcccgggt caagctgctc ccgccggatg agctgctgaa gcggctgggc
     8881 gacagcctcg ccctgctctc gggcggcgct cgggatctcc cggagcggca gcagaccctt
     8941 cggggagcga tcgcctggag ctacgacctc ctcgatgagc gggaacgctc gttcttccgg
     9001 cggctctcgg tgttcatggg gggcttcacc ctggaggccg ccgaggaggt gtgcggcggc
     9061 gccagagagc tcggggacac gctcgatctc gtggcctcga tgctcgacaa gagcctggtg
     9121 cggaccttca cgggcccggc cgggagccgc tacatgatgc tccagacgat cggcgagtac
     9181 gcgcgggagc tgctggaaga ttcgggcgag gccggggagg ttcgggcccg ccacgcacga
     9241 ttcgtggagc ggatcctgga ggatgccgtg cccaagctat tcggcccgga ccgcgccctg
     9301 tggctggacc gtctggcggc cgagcacgac aacctccgtg ccgcgctcac ctgggcgctc
     9361 gaggcgggag acctcgaggc gggcctccgg ctgggcggaa gctcgtggcg attctggcag
     9421 atgcgcgggc atctccgaga aggccgggaa tggctcgggc ggctgttggc ccatccgacg
     9481 gcggtcgacc acccgggccc gcgagcggat gctttggagg ggctgggggg gatcctctac
     9541 tggatggggg acgaggaggc ggcggaggtg tacgaggagt gcctgcggct ccgtcgagag
     9601 ctcggtgacg ccgctgcgat cggagaggcg ctctacaacc gcgccagtct gaacgtcttc
     9661 ggcatcggcg gagcccagga ctcgcaggcc gcgctgcccc tcctcgagga ggctctgggg
     9721 atcttccgcg agctggggga gggggtgggg gtggccaagg tgcagtgggc ggtgggcagc
     9781 gcccatctcc acgatgggga ctatgacgcg gccgagtccg cgctcagcga gagcatcgac
     9841 ctctatcgcg aggcgcagga tttgttcggt gaggcgtggg gcctccacat gctggcggtg
     9901 acgctgctcc acgaggggag gggcgaggag gcgctgccgc acctccggcg tgccgtcgac
     9961 atcttcgatg aggcgggcga ccgatcggcg atcccggtgc tcctcttcga ctccgcgatc
    10021 tacgcgtacc agcatggcga ccgtgaccga gccctgttcc tgatgagcgt cgcccagaag
    10081 gtccagcagg agacgggcgt cgggctcggg gaggcttccg tcgagttcgt gggactcctc
    10141 aggcccctga tggagaccat gtcggacgag gaaggcgaac gagctgcggc cgaggcccgc
    10201 gacatcaccg aggagcaggc cgtggagatg atcagggagc tgtgttcggg gtagttgcta
    10261 gagtggacga ggcgacggac caggaggtgc cgggcatggc tcagcaggag gacttcctcc
    10321 ccctcatggg gatcgatcac gtcgagtact acgtcggcaa cgcccgccag gccgcttact
    10381 actactcgac ggccttcggc ttcaaggtca cggcctaccg cggtccggag acgaagcacc
    10441 gggacgccgt ctcgtggctg tgcgagcagg gcgacgtgcg cttcgtgttc acgggggccc
    10501 agggtcccga gcacccgatc gcgcggcacg tcctcgagca cggcgacggc gtcaaggaca
    10561 tcgcgctgcg cgtgcccgac gccacctccg catgggagga gacgacgaag cgcggcgcgg
    10621 agagcgcgat ggagccccag gtcttcgagg acgagcacgg caaggtggtg cgctcggcga
    10681 tcaaggccta cggcgagacg ctccactcct tcatcgagcg ggagaactac tccggcccct
    10741 tccttccggc cttccgccag gaggagccgt accccgacga cggcccggag gtgggcatca
    10801 aggcgatcga ccactgcgtg gccaacgtgg agctcggcaa gatgaacgag tgggtgcgct
    10861 tctacgccga ggtcatgggc ttctcccagc tgatgcactt ccgagacgat cagatctcga
    10921 ccgagtacac ggcgctgatg tcgaaggtga tgtgggacgg atcgggccgc gtgaagttcc
    10981 ccatcaacga gccggcggag ggcaagaaga agtcccagat cgaggaatac ctcgactact
    11041 accgttcgcc tggggtccag cacatcgcga tgcagaccga ggacatcgtg cagaccgtga
    11101 ccgccctgcg ggagcgcggc gtgcgcttca tccgtgtgcc cgagacttac taccaggacc
    11161 tcgccgagcg cgtgggcgag atcgacgagg actactcgga cctccagaac ctggcgatcc
    11221 tcgccgaccg cgacgaggag ggctacctgc tgcagatctt cacgaagatc cagcaggacc
    11281 gccccacggt cttctacgag ttcatcgagc gccacggctc gcgcggcttc gggctcggga
    11341 acttcaagga gctcttcatc gcgatcgagc gggagcaggc cgagcgcggg aacctctgag
    11401 ggcggaccga gcgtcccccg ttcgccgggc cgaaccgccc tgtccggctc ggcggagagc
    11461 ctccaggctt gatccgaggc aggaggtgac ggcgatgcgc atcctcgtca ggcacgtgat
    11521 gacccacgac gtggccaccg tctcgccctc ggcgggatgc caggagatcg cccagctgat
    11581 ggtggaccgc cacgtgcgga cggtccccgt ggtggaggcc gacgggacgc tcgtagggct
    11641 cgtgacagag gccggtctgc tcgagcacgt ggggggtgcg agagagcccc tccacaccgt
    11701 cgagttgctg tccactccgg gtcggcaccc acacccgctc ccgtgcgagc tgcacgcgac
    11761 ggacgtcatg acgcgcgggg tggtcacgag ccggcgcgac gcgaccgtgc cggaggcgat
    11821 cgacgccctc gtggccgcga gggtccgcgg gctccccgtc gtggacggca atggggtgct
    11881 ccttgggatc gtggatcgag gcgatctcct gcgcggcctg gcccccatga tccgctcagt
    11941 agacggtgtc gtcggtctgc accccgccca cggtcgtcgg cgcgctcgcc gtgagggcct
    12001 gcgggcgtcg tgagctaggc cagaccggtg atgccccgcc aggccaggcg gaccagcgcg
    12061 tccacggtgc ggcgccgatc ctccttggag gcgccggggt tggcctgggc ggccgtctcg
    12121 gccatcccga tcagccccag cgcgacgtcg cgcgcatcct ccatcgggac cctcccccgg
    12181 gactcccggt ggatcacctc ggtcacctcg gcgtggacgt cgtcgcggaa gcgctccacc
    12241 atctcgcgga agtcgcgctc ggagccgacc gcctcggtca tcagcaggcg gaacccctcc
    12301 gcgtgctcgt ccacgaagtc gaagtagacc tggaacgagg cccggatccg ctcgcggttc
    12361 ccccgcgacg tctcgagggc ggtgcgcacg cgctcccgca gcgacgcgag atctgcctcg
    12421 agcagggcca ggtagagatc tcgcttggag gagaagtggt cgtagaggat cggcttggtg
    12481 accccggcct ccttggccac ggcgtccatc gtcaccgtgt ggaagccgtt ccggccgaag
    12541 accttccggg ccacgtcgag cagctgctgt ctccgctccg gagcccgcat ccgccgccgc
    12601 tgcgccattg accttccccc tacctgctgg taacctaccc tccagtagga taggcacccg
    12661 ggccgctcgt cattctgcca gagaggaccg cccgatatgg agttcaggct caacgaggat
    12721 cagctcaccg cccagaagtg ggcccgcgag ttcgcggaga aggagatccg tccggtcgcc
    12781 ccggagtacg acgagtccga ggagttcccc tggccggtcc tgaagaaggc caccgaggtc
    12841 gggctcaact cgctcgagtt ctacgtggag atggccgggg ccgaccccac cggcctcatc
    12901 atgccgatcg tcctcgagga gtcgtgctgg ggctgtgcgg gcatctcgct cggcatcttc
    12961 ggcaccgggc tcccgctggc cacgctggcc ggcaccggga cccccgatca gttcctcgag
    13021 tgggccccgc ggatgatcgg gactcccgat catccgaagg tcggggcttt cgccgtcacc
    13081 gagtcgggcg ccggttccga cgtctccgcg ctccgcacca ccgcgaagcg cgacggcgac
    13141 gagtgggtgc tcaacggtca gaagatcttc atcaccaacg gcgggatcgc cgacgtccac
    13201 atcgtggtgg ccaccgtcga tccggagctg gggcatcggg gccaggcagc cttcatcgtc
    13261 gggccagaca caccggggat ctcccagggg aagaaggagc ggaagctggg gatccgggcc
    13321 tcgcacacgg cggaggttct cctcgacgac tgtcgggtcc cgctggagaa cgtggtgggc
    13381 gggatggaga ggctcgaagc gaagctggcg cgggggcggg agaagggcgg cgagccgtcc
    13441 cggtcctcgc aggcgctgaa gaccttcgaa ctgacacggc cgctggtagc ggcccaggcc
    13501 ctggggatcg cacgggccgc gctcgagtac tccgtggact acgcgaagga gcggacgacg
    13561 ttcgggcgac cgatcatcga gcaccagggg atcgggttca agctggccga catggcgatg
    13621 gaggtcgacg cggcgcgcct cctgacgtgg cgggccgcct ggatggtgag gaacgatcag
    13681 ctccagcggg ccgagggctc gatgtcgaag ctcaaggcct ccgaggtcgc ggtctgggtc
    13741 accgagcagg cgatccaggt cctcggcggc tacggctaca tcaaggactt ccccgtcgag
    13801 aagtggtacc gggacgccaa gatctacacc ctgttcgagg ggaccagcga gatc
//
LOCUS       DASUTZ010000249         3006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1647926, whole genome shotgun sequence.
ACCESSION   DASUTZ010000249 DASUTZ010000000
VERSION     DASUTZ010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1647926"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..1390
                     /locus_tag="VF097_10640"
     CDS             50..1390
                     /locus_tag="VF097_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX6263291.1"
                     /translation="MDRLDQLIEEQERIFLDRQPASRALTEEASAHLAGGVTSSWQIS
                     RPGTVWLDRAEGSRVWDVDGNEYVDLHGGYGVNLAGHGHPAIARAIAEQAPRGTHFAQ
                     PVDDALIVARELSRRWGLPLWRFNNSGTEATMDAFHLMRAATGRDLIVKAEGAYHGHH
                     DAAMVSVYNELEELGPREAPVSVSSGAGIPQAFIDLTLTVPFNDLDALNRVLSEHDAA
                     GVILEPVMMNAGIILPEEGYLEGVREITRRHGVLLTFDEVKTGLTVGPGGAARRFGVA
                     PDIVCLAKAMGGGVPCGAIGGTEEVMRVIAEGEYEQVGTFNGNPLTMAAARATLTEVL
                     DEEAYARLDALAERMAKGSDEVIERYGLPAHVVAIGAKGCLTFSEAPVRDYRDFLAVD
                     DRWSHCHFLFQHNGGVFLPPWGKAEQWLLSVQHTEDDVDRFLENLERFAAAVGG"
     gene            1393..2472
                     /locus_tag="VF097_10645"
     CDS             1393..2472
                     /locus_tag="VF097_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6263292.1"
                     /translation="MAGGHVELVGLVKRFADVTAVDGIDLEVAPGEFFSLLGPSGCGK
                     TTTLRMIAGFERPDEGRILLDGQDVAMTPPHKRNVNTVFQSYALFPHLSVRDNVAFGL
                     RFQDVGKEDTDRRVSEVLRLVELSGFEDRKPAQLSGGQQQRVALARALVLNPAVLLLD
                     EPLGSLDAKLRKALQIELKSIQEEVGITFIYVTHDQEEALTMSDRLAVMSAGRIRQMG
                     TPKEVYETPADAYVADFLGVSNLMDAVGDGSDGAVRIGQSTLRTTGTAGPGAPVRVVI
                     RPERVRVEEQGAVGENRLPGMVERTVYVGSTMQVIVRLPEGQTVQAMIPNRGDEHGYQ
                     QGTPVTLHLPAEDLRVLEDEPVENS"
     gene            2560..>3006
                     /locus_tag="VF097_10650"
     CDS             2560..>3006
                     /locus_tag="VF097_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEX6263293.1"
                     /translation="MDGLLPVPEPWRVKSVEPIRLLPEREREAALERAGWNVFRLRAE
                     EVFVDLLTDSGTAAMSVSQWAGLMLGDEAYAGSAGWTHLEDVVRRVTGYPHVIPVHQG
                     RGAETVYTRAFVREGDLVLGNIHFDTTRAHIRNRGAEPVDLVVPEGL"
BASE COUNT          456 a         1021 c         1103 g          426 t
ORIGIN      
        1 gatcggtccg tatgatattg aatggccgtt caaagatgaa ggagcgagca tggatcgtct
       61 cgatcagctg atcgaggagc aggagcggat cttcctcgac cgtcagcccg cctccagggc
      121 cctcaccgag gaggcctcgg cccacctggc cggaggggtg acgtcgagct ggcagatctc
      181 caggcccggc accgtctggc tggaccgagc cgaggggtcc cgggtgtggg acgtcgatgg
      241 gaacgagtac gtcgacctcc acggggggta cggcgtgaac ctggccgggc acgggcatcc
      301 cgcgatcgcc cgggccatcg ctgagcaggc gccgcgaggg acccacttcg cccagccggt
      361 ggacgacgcg ctgatcgtgg ccagagagct gagccgacgc tggggcctcc cgctgtggcg
      421 gttcaacaac tcggggaccg aggccacgat ggacgcgttc cacctgatgc gcgccgccac
      481 cggccgcgac ctgatcgtga aggccgaggg ggcctaccac ggccatcacg acgcggcgat
      541 ggtctcggtc tacaacgagc tggaggagct ggggccccgc gaggctccgg tgagcgtgtc
      601 ctccggcgcc gggatcccgc aggccttcat cgatctgacc ctcacggtcc ctttcaacga
      661 cctcgacgcg ttgaaccgcg tgctctccga gcacgacgcg gcgggggtga tcctggagcc
      721 ggtgatgatg aacgctggga tcatcctccc cgaggagggc tacctggagg gggtccgcga
      781 gatcacgcgg cgccacggcg tcctcctgac cttcgacgag gtgaagaccg gcctcaccgt
      841 ggggcccggc ggggccgccc ggaggttcgg cgtggcgccc gacatcgtat gcttggccaa
      901 ggccatgggc ggcggcgtac cctgcggggc gatcgggggg accgaggagg tcatgcgggt
      961 gatcgccgag ggcgagtacg agcaggtggg gaccttcaac ggcaacccgc tcacgatggc
     1021 cgccgcccgc gccacgctca ccgaggtcct cgacgaggag gcctacgcgc gcctcgacgc
     1081 cctggccgag cggatggcga agggcagcga cgaggtgatc gagcggtacg gcctcccggc
     1141 gcacgtcgtc gcgatcgggg ccaagggctg cctcacgttc tcggaggcgc ccgtgcgcga
     1201 ctaccgggac ttcctcgcgg tggacgaccg ctggagccac tgccacttcc tgttccagca
     1261 caacggcggc gtgttcctcc cgccctgggg gaaggccgag cagtggctgc tctcggtcca
     1321 gcacaccgag gacgacgtgg accggttcct ggagaacctg gagcgtttcg ccgcggccgt
     1381 cggggggtga ggatggccgg gggacacgtc gagctcgtcg ggctggtcaa gcggttcgcc
     1441 gacgtgaccg cggtggacgg catcgaccta gaggtggcgc ccggcgagtt cttctcgctc
     1501 ctcggaccgt ccggctgcgg gaagacgacg accctccgga tgatcgccgg cttcgagcgt
     1561 cccgacgagg gccgcatcct gctcgacggg caggacgtgg ccatgacccc tccccacaag
     1621 cggaacgtca acacggtctt ccagtcctac gcgctcttcc cgcatctctc ggttcgcgac
     1681 aacgtcgcgt tcggcctccg gttccaggac gtggggaagg aggacaccga tcgacgggtc
     1741 agcgaggtgc tgcgcctggt cgagctctcg gggttcgagg accgcaagcc cgcccagctc
     1801 tcgggcggcc agcagcagcg cgtggcgctg gcccgggccc tcgtgctgaa ccccgcggtg
     1861 ctgctgctcg acgagcccct ggggtcgctc gacgcgaagc tccggaaggc cctgcagatc
     1921 gagctgaagt cgatccagga ggaggtgggg atcaccttca tctacgtcac gcacgaccag
     1981 gaggaggccc tcacgatgtc cgatcggttg gccgtgatgt cggccgggcg gatccgccag
     2041 atggggaccc ccaaggaggt ctacgagacg ccggccgacg cctacgtcgc cgacttcctc
     2101 ggcgtctcca acctgatgga cgccgtgggg gacggctcgg acggcgccgt ccggatcggg
     2161 cagtcgaccc tccgcaccac ggggacggcc ggccccgggg cgcccgtgcg cgtcgtgatc
     2221 cggccggagc gggtccgggt tgaggagcag ggcgccgtcg gcgagaaccg cctcccgggc
     2281 atggtcgagc gcaccgtcta cgtcgggtcc acgatgcagg tgatcgtccg cctccccgag
     2341 ggccagacgg tccaggcgat gattcccaac cggggggacg agcacggcta tcagcagggc
     2401 acgcccgtca cgctgcacct ccccgccgag gacctccgag tcctcgagga cgagccggtc
     2461 gagaactcct gatcccaccc ccccagggac cttcggcccg gtcgtgatgg gaccctcgtc
     2521 cctcccggcg gcccatcgcg acggggagag tcgggatcga tggacggact gcttcccgtg
     2581 cccgagccct ggcgggtgaa gtcggtcgag ccgatccggc tgctgccgga gcgggagcgc
     2641 gaggccgcgc tcgagcgcgc cggctggaac gtattccgac tgcgcgccga ggaagtcttc
     2701 gtggacctgc tgacggactc gggcaccgcg gcgatgagcg tgtcgcagtg ggcggggctg
     2761 atgctcgggg acgaggccta cgcgggatcg gcgggctgga cccacctcga agacgtcgtc
     2821 cgccgggtca ccggataccc gcacgtcatc cccgtccacc aggggcgcgg ggcggagacc
     2881 gtgtacacga gggcgttcgt gcgcgagggc gacctcgtgc tgggcaacat ccacttcgac
     2941 acgacccggg cgcacatccg caaccggggg gcggagcccg tggacctcgt ggtgccggag
     3001 gggctc
//
LOCUS       DASUTZ010000250         2834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_481850, whole genome shotgun sequence.
ACCESSION   DASUTZ010000250 DASUTZ010000000
VERSION     DASUTZ010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481850"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..469)
                     /locus_tag="VF097_10655"
     CDS             complement(47..469)
                     /locus_tag="VF097_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007297517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX6263294.1"
                     /translation="MLMRWDPFGELDRLTRWAGETGRRPAVAMDAFRRGDRFVMTFDV
                     PGIDPDSLDLTVQDNVLTLRAERRTTLDEYDELLVHERPVGSYTRQVFLGENLDTEKL
                     EADYEQGVLTVIVPVAEAARPRKIEIGSKSKAIDAGAS"
     gene            complement(605..1432)
                     /locus_tag="VF097_10660"
     CDS             complement(605..1432)
                     /locus_tag="VF097_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263295.1"
                     /translation="MVLDRHWLLGVARREREALGRTIQYTDLGAWENPGPWQGRPIIE
                     ILYHLAATEIAAAEVWGGEAPAELDEYRKAAGEDASPDGFTHWALARRAEQSAVQTAR
                     EWGRAADLLLDRATATSDEDWRDRMIPWFEGDLRAGYFLQYRVAQWWLHGQDILDGSA
                     QALRREHDPTFVVNDFAVRLIPYALSLTAEDPGELVVRVDLDGVGGGVWLQSTRPGPP
                     PPEDATPSVIIGGRGPWLALVASGRVDPDVALYEGVLNVGGDLAGGEMILRSLRAYP"
     gene            1492..2607
                     /locus_tag="VF097_10665"
     CDS             1492..2607
                     /locus_tag="VF097_10665"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX6263296.1"
                     /translation="MRVLMSPDKFKGTLTAPAAAEALATGWRRSRPRDDVVELPVADG
                     GEGTLSTLVAALGGEVRGAKVTGPLGDPVEAEFGLVETPEGREAIVEMARASGLELVS
                     EPRRDPRRATTRGTGELILEAVEAGATRILVCIGGSATNDAGAGMAQAVGVRLYDEAG
                     EELPLGAEALDRLARIDLRGLDPRVAKASVVVACDVDNPLTGPRGASAVYGPQKGASP
                     EDVAFLDRCLSRFSAVAGRDLGLDLRGVEGAGAAGGMGGGLIAFLGARLRPGFELVAE
                     ALDLSRHVAGSDLVITGEGRFDRQSLSGKAPAGVLWLAREAGTRAFVVAGQVEDGVEI
                     PAERVMDLSARAGLEAAMDRPRTALADAAADLAEEVA"
     gene            2619..>2834
                     /locus_tag="VF097_10670"
     CDS             2619..>2834
                     /locus_tag="VF097_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018838437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX6263297.1"
                     /translation="MSAVDRFRAEAAARGLGELDVREFPEGTRTAADAARAIGCEIGQ
                     IVKSVVLMADGAPVVALTSGANRADTAK"
BASE COUNT          383 a          963 c         1106 g          382 t
ORIGIN      
        1 gcgcggcgag ttcctcgccg cgcgccccgc cccaaaggct ggcgccttac gaggcgccgg
       61 cgtcgatcgc cttggacttc gacccgatct cgatcttccg cggcctggcc gcctccgcga
      121 ccggcacgat gaccgtcagg acgccctgct cgtagtccgc ctcgagcttc tccgtgtcga
      181 ggttctcccc caggaagacc tgcctcgtgt aggagcccac gggccgctcg tggaccagga
      241 gctcgtcgta ttcgtcgagc gtggtcctgc gctccgcccg gagcgtcagc acgttgtcct
      301 ggaccgtcag gtcgagggaa tcggggtcga tcccggggac gtcgaacgtc atgacgaacc
      361 ggtccccgcg cctgaaggca tccatggcca ccgccgggcg acggccagtc tcgccggccc
      421 agcgggtcag gcgatcgagc tcaccgaacg gatcccaccg catcagcatg gctccaccac
      481 ctcctctcag gacacctgat tggagccttg ttccgtacgc agtatagaaa cctaagcgca
      541 aggctgtcaa cccctgggct cacgacggca ccatcgcagg gagcacgagg ggcggcgcgc
      601 gtcttcaggg ataggctcgc aggcttcgga ggatcatctc gccgcccgcg aggtcgcccc
      661 cgacgttgag gacgccctca tacagcgcca cgtcggggtc cacccgtccg ctggccacga
      721 gggcgagcca gggccccctc cctccgatga tcacgctcgg cgtggcgtcc tcgggcgggg
      781 gcgggccggg gcgggtggac tggagccaga cccccccgcc caccccgtcg aggtcgaccc
      841 gtacgaccag ctcgcccggg tcctcggcag tcagcgagag ggcgtacggg atcagccgaa
      901 ccgcgaagtc gttcaccacg aacgtcggat cgtgctcccg gcgcagggct tgggcgctcc
      961 catcgaggat gtcctggccg tggagccacc actgcgccac gcggtactgg aggaagtagc
     1021 cggcccggag gtccccctcg aaccagggga tcatccggtc ccgccagtcc tcgtccgagg
     1081 tcgcggtggc gcggtcgagc aggaggtcgg cggcccgccc ccactcccgc gccgtctgga
     1141 cggccgactg ctccgcccgg cgggccaggg cccagtgcgt gaagccgtcc gggctcgcgt
     1201 cctctccggc ggccttgcga tactcatcga gctcggcggg ggcctcgccg ccccagacct
     1261 cggcggcggc gatctcggtg gcggccaggt ggtagaggat ctcgatgatg ggtcgcccct
     1321 gccacgggcc ggggttctcc caggcaccga ggtcggtgta ctgaatcgtc ctgcccaggg
     1381 cctcgcgctc gcggcgcgcc acgcccagca gccagtgtcg gtccagcacc atctcgggcg
     1441 gaatcataca ggcgcccaga ggcctcgccg aaatcccgct aggctcgaac catgcgcgtc
     1501 ctgatgtccc ctgacaagtt caagggaacg ctgaccgcgc cagcggcggc cgaggcgctg
     1561 gccaccgggt ggcggcgttc gcggccgcgc gacgacgtcg tggagctgcc ggtcgccgac
     1621 ggcggcgagg gcacgctctc caccctcgtg gcggcgttgg gaggggaggt ccgcggggcg
     1681 aaggtgaccg ggccgctggg ggatccggtg gaggcggagt tcggtctcgt ggagaccccg
     1741 gaggggaggg aggcgatcgt ggagatggcc cgcgcctcgg gcctcgagct cgtctccgag
     1801 cccaggcggg acccgcggag ggcgaccacg cggggcacgg gggagctgat cctcgaggcg
     1861 gtggaggcgg gggccaccag gatcctcgtc tgcatcgggg gaagcgccac gaacgacgcc
     1921 ggggcgggga tggcccaggc cgtgggggtc cgcctctacg acgaggcggg ggaggagctc
     1981 cctctcgggg ctgaggccct cgaccggctg gccaggatcg atctgcgggg gctcgatccc
     2041 cgggtggcga aggcctcggt cgtcgtggcc tgcgacgtgg acaacccgct gacggggccg
     2101 cgcggcgcga gcgcggtgta cgggccgcag aagggggcct cgcccgagga cgtcgcgttc
     2161 ctcgaccgat gcctgagcag gttctcggcc gtcgcgggga gagacctcgg gctcgacctg
     2221 cggggcgtgg aaggggccgg cgcggccgga ggcatgggag gcgggctgat cgcgttcctg
     2281 ggcgcccgcc tccggcccgg cttcgagctg gtggcggagg ccctggacct gtcgcgacac
     2341 gtggccggat cggacctcgt catcacgggg gagggacgat tcgaccggca gtcgctttcc
     2401 gggaaggcgc cggcgggcgt gctctggctg gcgcgggagg ccgggacccg ggcgttcgtg
     2461 gtggcggggc aggtggagga cggggtggag atcccggcgg agcgggtcat ggacctgtcg
     2521 gcccgggccg ggctcgaggc ggcgatggat cggccgcgga cggcgctggc ggacgccgcc
     2581 gcggatctgg cggaggaggt ggcgtgagcg agcggaaggt gtcggcggtg gaccggttcc
     2641 gggcggaggc cgcggcgagg gggctgggag agctcgacgt cagggagttc cccgagggga
     2701 cgaggacggc ggccgacgcg gctcgggcga tcggctgcga gatcggccag atcgtgaaga
     2761 gcgtggtgct gatggcggac ggcgctccgg tggtcgccct cacctcgggc gccaaccggg
     2821 cggacacggc caag
//
LOCUS       DASUTZ010000251         2398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_612666, whole genome shotgun sequence.
ACCESSION   DASUTZ010000251 DASUTZ010000000
VERSION     DASUTZ010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612666"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VF097_10675"
     CDS             complement(<1..576)
                     /locus_tag="VF097_10675"
                     /inference="COORDINATES: protein motif:HMM:NF024776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6263298.1"
                     /translation="MSAQPDHARREMARSLRRAGYSRAQIARRMGLKSSGGALGRWLT
                     GVPPPAWTRRPRAKDHLRQEAVRLRRTGASYREISDELGVSKSTLSAWLHDVPLTEEH
                     RETLALRQRSAVQRRADALRAASRARHRSLVRESEARVGGLSERELFVAGVVAYWAEG
                     AKTKPWGARSLVRFSNSDAGMIRLFLAWLDLV"
     gene            complement(614..1444)
                     /gene="ispE"
                     /locus_tag="VF097_10680"
     CDS             complement(614..1444)
                     /gene="ispE"
                     /locus_tag="VF097_10680"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX6263299.1"
                     /translation="MTVIREPAPAKLNLFLRVLGRRDDGYHEIETLVLPLTLADGVEA
                     AVADGLSAAAAGPHASGVPIGEENLVVRAARALAGACGVEPRARLLVAKNVPVAAGLG
                     GGSADAAAALRALNDLWTCGLGVEELAVVAAGVGSDVPALVQGRPVLARGRGEVVVPF
                     DLPRLWWVVVPREGVRAADAYARWDEEGSTGPDTEGLLAAASAGDLEGIAAGVFNDLD
                     TGAVPEVEEAKRSLLDAGAAAALMCGSGPSVAGLCRDGAHAEEVAAAVEGIACATVRR
                     "
     gene            complement(1441..2214)
                     /gene="rsmA"
                     /locus_tag="VF097_10685"
     CDS             complement(1441..2214)
                     /gene="rsmA"
                     /locus_tag="VF097_10685"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX6263300.1"
                     /translation="MRPSKALGQHFLADPNLARAIVREAVVEPEDRVLEIGAGLGSLT
                     LALIQAGAHVLAVELDGALLPALREVVGGSDRVRVEQVDAMTADWGALLGQERWRMVS
                     NLPYNVAVPVVVRLLEEAPSVDPMLVMVQREVGERLAAGPGEEAFGAVSLRVAYRAEA
                     WVVRRVPKTVFWPEPKIESVLVRLGRRSPPVDVPPEELFRLVDEGFAQRRKTMANALV
                     RLGLGREAAAAALDAAGLDPKVRAEELGLEDFARLAGAL"
     gene            complement(2264..>2398)
                     /locus_tag="VF097_10690"
     CDS             complement(2264..>2398)
                     /locus_tag="VF097_10690"
                     /inference="COORDINATES: protein motif:HMM:NF015298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX6263301.1"
                     /translation="GRSVWVTIDDRGPYIDGRIIDLSDDAFLQLAPLGAGTINVRITW
                     "
BASE COUNT          354 a          987 c          761 g          296 t
ORIGIN      
        1 aacaaggtcc agccaggcca agaacaagcg gatcatcccg gcatctgagt tgctgaacct
       61 cacgaggctg cgtgcgcccc agggcttggt cttcgctcct tcggcccagt acgcgaccac
      121 tcccgcgacg aagagctccc gttcggagag gccgccgacc cgcgcctccg attcccgcac
      181 gagcgatcgg tggcgggctc ggctcgcggc acgaagcgca tcggccctcc gctgaacggc
      241 agatctttgc ctcagagcca gcgtctctcg atgctcctcg gtgagaggga cgtcgtgcag
      301 ccaggcggac agggtgctct tcgagacgcc gagctcgtca gagatctcac ggtaggaagc
      361 accggtgcga cgcaggcgga cggcctcctg gcgaaggtgg tccttcgcgc gagggcgtcg
      421 cgtccacgcg ggcgggggga cgccggtcag ccaccgaccc agcgccccac ctgagctctt
      481 gagtcccatc cgccgagcga tctgggcccg ggagtatccg gctcggcgga ggctccgggc
      541 catctcgcgg cgagcgtggt cgggctgagc ggacatgcat cgagcatacc gggcgatacc
      601 gacacgaacg tcttcagcgt cgaaccgtcg cgcacgcgat cccctcgacg gcggccgcca
      661 cctcctcggc atgagccccg tcccgacaca gcccggcgac gctggggccg ctgccgcaca
      721 tcagggcggc agcggcccca gcgtccagca gcgatcgctt cgcctcctcg acctcgggga
      781 ccgcccccgt gtcgaggtcg ttgaacactc ctgccgcgat cccctccagg tcgcccgccg
      841 acgccgccgc cagcagcccc tccgtatcgg gaccggtcga cccctcctca tcccaccggg
      901 cgtacgcgtc ggcggcccgc acgccctcgc gcggcaccac cacccaccac agccggggga
      961 gatcgaacgg cacgacgacc tcgcccctcc cccgagccag caccggacgc ccctggacga
     1021 gcgcggggac gtccgatccg acgcccgccg ccaccaccgc cagctcctcg actcccaacc
     1081 cgcaggtcca gaggtcgttg agggctctga gcgccgcggc cgcgtccgcg ctcccccctc
     1141 cgaggccggc cgccaccggg acgttctttg cgacgagcag acgggcccgg ggctcgaccc
     1201 cgcacgcccc cgccagggcc ctcgcggccc gcaccacgag gttctcctct ccgatcggta
     1261 cgcccgaagc gtggggaccg gcggccgcgg ccgacaaccc gtcggccacg gccgcctcca
     1321 ccccgtccgc cagcgtcaag ggcaagacga gcgtctcgat ctcgtggtag ccgtcgtccc
     1381 tccgccccag cacccgcagg aacaggttga gcttcgccgg ggccggctcc cggatcaccg
     1441 tcacagcgcc cccgccagac gggcgaagtc ctcgaggccc agctcctcgg cccgcacctt
     1501 cggatcgagc cccgccgcgt ccagcgcggc ggccgccgcc tcgcgcccca accccagccg
     1561 caccagcgcg ttcgccatcg tcttcctccg ctgggcgaag ccctcgtcca ccagacggaa
     1621 cagctcctcc gggggcacgt cgaccggcgg cgaccgccgc cccaaccgca cgagcaccga
     1681 ctcgatcttc ggctccggcc agaacaccgt cttcggcacc cgccgcacga cccacgcctc
     1741 ggcccggtag gcgacccgga ggctcaccgc cccgaacgcc tcctcccctg gccccgccgc
     1801 gaggcgctca ccgacctccc gctggaccat caccagcatc gggtccacgc tcggcgcctc
     1861 ctccaggagc cgaacgacca ccggcacggc cacgttgtag gggaggttcg agaccatccg
     1921 ccagcgctcc tggcccagca gggcgcccca gtccgcggtc atcgcgtcca cctgctccac
     1981 gcgcacccgg tccgacccgc ccaccacctc ccgcagggcg ggcaggaggg cgccgtcgag
     2041 ctccacggcg agcacgtggg cccccgcctg gatcagcgcc agcgtcagcg acccgagccc
     2101 ggctccgatc tcgaggaccc ggtcctcggg ctccacgacc gcctcgcgga cgatggcgcg
     2161 cgccaggttc ggatcggcca ggaagtgctg gcccagcgcc ttcgacggcc ggatcccgtg
     2221 ccgctcggcg agggcgcgga gaacccgctt cgtgagaagg ccgctaccag gtgatccgga
     2281 cgttgatcgt ccccgcgccg aggggggcga gctggaggaa ggcgtcgtcg gagaggtcga
     2341 tgatccgtcc gtcgatgtac ggtcctcggt catcgatcgt cacccagacg cttctccc
//
LOCUS       DASUTZ010000252        14704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_94413, whole genome shotgun sequence.
ACCESSION   DASUTZ010000252 DASUTZ010000000
VERSION     DASUTZ010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14704
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94413"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..1028)
                     /locus_tag="VF097_10695"
     CDS             complement(171..1028)
                     /locus_tag="VF097_10695"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX6263302.1"
                     /translation="MPVVVVGASSSVGRALVPLLVERTPEVRAIVRSPTQAEGLRALG
                     AKVAVARAPDADALEMLFHDAHTVCHLAGGIRMRSAQAYQEANLETVRRTVEGARRAG
                     AERILLLSTSGADPEDPNAYLRSKGRAEALVRKSDLEHAIVRATRLVAPASPWFRDVA
                     ALAARRPAAVVGDGSVSLAPVASADVAAILAAADDRAGSISTTLGVEGPDVVTADELV
                     RIVAGRRRRPRHLAPGARASRALGGPVAPDYLDLLALDARADAPDAAAEFDVLLTPLR
                     EAIGWAVRG"
     gene            complement(1032..1952)
                     /locus_tag="VF097_10700"
     CDS             complement(1032..1952)
                     /locus_tag="VF097_10700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6263303.1"
                     /translation="MARQDLHPGDVDDLRLYLEAAAREPLLTKEEEVELAMSLEHGKE
                     AEERLASGRIRSEKTKATLAADIKEAERSRKRFILANLRLVVSIAKKYQGQGLPLLDL
                     IQDGNIGLMRAIELFDWRRGFKFSTYATWWIRQAITRAIADRGRQIRIPVHVHERIRK
                     IRKTRERLMQQTGRDPTPDELSRAAGVPVEMLEELEAAVRKEPRSLQEPVGEDAELGD
                     LVAQVELESPLDAVEDTLLREEITQAVGTTLEPMEQEVVSLRYGLGNGRAMSLREVAE
                     LVGVSAETVRKVERQALGKLRASDLLEEIW"
     gene            complement(1995..2504)
                     /locus_tag="VF097_10705"
     CDS             complement(1995..2504)
                     /locus_tag="VF097_10705"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEX6263304.1"
                     /translation="MALIQMRAPEGRPWKLKEWVQGKPLGVPTHSMVVHFPVAFYLGV
                     IVFDVMSRITPNEGLVLASTYMLLLGLLGTLVAAPLGLIDWLGMVKGSSKRRLATRHM
                     VFQLLAAAFFIVAFVLRWPDRTASQAEISWIVIEAVGYLVLIVGQHLGGTLVYQKAMR
                     VSTGGPDRE"
     gene            complement(2563..2715)
                     /locus_tag="VF097_10710"
     CDS             complement(2563..2715)
                     /locus_tag="VF097_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263305.1"
                     /translation="MCLTCGCGEPNADHGDGRHITLNDLQAAAEAAEISADEAASNLQ
                     ETYKNA"
     gene            complement(2808..3398)
                     /gene="rdgB"
                     /locus_tag="VF097_10715"
     CDS             complement(2808..3398)
                     /gene="rdgB"
                     /locus_tag="VF097_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX6263306.1"
                     /translation="MSRAMPDRLVIASRNPGKIREIQAIASDWPVEWVTDADWPEVDE
                     TGRTYLQNAKRKAHEVAEATGLPALADDSGIEVDALGGAPGPRSARFAGEGADDARNL
                     RELLRAVAGVPGPGRTARYRAVAVIAWPDGGEVWAEGDCEGLLRDEPSGSGGFGYDPI
                     FEPAGWDRTMAELSPEEKDRISHRGRALRALRQALR"
     gene            complement(3395..4114)
                     /gene="rph"
                     /locus_tag="VF097_10720"
     CDS             complement(3395..4114)
                     /gene="rph"
                     /locus_tag="VF097_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX6263307.1"
                     /translation="MSRPDGRGPRELRPIRCETGYLAFAEGSVLLEMGGTRVLCAATW
                     EPRVPRWLDGTGKGWVTAEYSMLPRSTKERTPREVNRGRPSGRTQEIQRLIGRSLRAV
                     TDLERLGEGSITVDCDVLQADAGTRTASITGGYVALALALRSLEEQGLARSGVLTDSV
                     AAVSTGIVGGVPLLDLCYDEDAEAEVDFNVVMTGSGKLVEVQGTAEGDPFSRDQLEEM
                     LTLAEEGIARLTEVQREALGS"
     gene            complement(4111..4869)
                     /locus_tag="VF097_10725"
     CDS             complement(4111..4869)
                     /locus_tag="VF097_10725"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6263308.1"
                     /translation="MELTVLGSDGTWPRADGAASGYIVSHDGFVVWQDMGTGTMAHLQ
                     RHLGLLDVDAVIVSHVHADHFVDLFPYFYARRYGAATPAPSIPLVVPTGTEERIRCML
                     SQAGWEEFPDSFQLHEIEPGAGLELGPFRVSTARMAHPVPTLGVRYEADGVALAYSAD
                     TGPTDELVDLARGVDLLVAEATWLEDGKDYPPDLHMTAKEAGEQAAKADAGRLMLAHI
                     QPIFDPDHSREKASEAFEGAVDVARPDVRWEAGA"
     gene            complement(4869..5690)
                     /gene="murI"
                     /locus_tag="VF097_10730"
     CDS             complement(4869..5690)
                     /gene="murI"
                     /locus_tag="VF097_10730"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018224973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX6263309.1"
                     /translation="MVGDARAIGMFDSGVGGLTVARSVLDLLPREPLVYYGDTARAPY
                     GPRPLDEIRAFSREIAAYLVHRDVKMLVVACNSVEVAAIEDVTAEAGVPVVGVIDPGV
                     RAAARVTRSGRIGVIGTEATIVSGAYHRAADHHGIDAELVARACPSFVPFVEGGDTTS
                     GALREAARGYLEPVRDAGVDTLILGCTHYPLLAGMIRMVMGPDVVLISSAEETARDVY
                     AVLVRDDLLREEQGLPRHEFLCSGDPDRFRELGRVFLGPEMDDVALASVQREPLG"
     gene            complement(5684..6616)
                     /locus_tag="VF097_10735"
     CDS             complement(5684..6616)
                     /locus_tag="VF097_10735"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEX6263310.1"
                     /translation="MRASDILEAIGNTPLVGIQRLAPKSEVRLWAKLEGQNPTGSLKD
                     RIAKAMVEAAETSGELTPDRVILEPTSGNTGIALAMVARRKGYGLMVVMPENASPERV
                     QLLELYGAEIVFSPADAGTNGSIEVARRMAEDPRYFMPFQYGNPANPRAHYEGTGAEI
                     VRDLPEATHFVAGLGTGGTLMGVGRRLKEHDPSVQVIAAEPELGDSVYGLRSLEEGFV
                     PPILEESLLDGKHLVAAGDALRATRMLAEQEGIFAGISSGAVLHVARKVGERVSEGDI
                     VCVLPDGGWKYLSTGAWTGDASEAEKRVEETVWW"
     gene            6776..7270
                     /locus_tag="VF097_10740"
     CDS             6776..7270
                     /locus_tag="VF097_10740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6263311.1"
                     /translation="MDEAGVAARVSFEAFYREQHARLYRALCLVTRNRHEAEDIAQDA
                     FLKLYERWERVATLEDPGGYLYRTAMNGFRSRYRRAALALKRGLGVVAADDGIDALEA
                     DDWVIRALAPLSRRQRAAVVLTDLLGYSSVEAGRLLALSPSTVRMHVSRAHAALRDRM
                     GDER"
     gene            7267..7986
                     /locus_tag="VF097_10745"
     CDS             7267..7986
                     /locus_tag="VF097_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263312.1"
                     /translation="MNVYGDLLERAGRRFPGPSDQLDDLYERWRRRQRNRRVVAGVTG
                     LVIFAVTAVTLGSRLRIGDDGTRPGGSSDRAVSAIVLGARDVQRGWELGETRLGRASV
                     TLIARGDTQVLLQRLPLIDGVSRRFRSEDPRPFAVLNAFVLRFGDEAAAQGGYALLEL
                     EFRRGDGSATELPGPLLGVESIALEQPQDRAYRAPATIYLWRRGDLVMGLMAIGSLQE
                     RQLEELARTMDARAEREDGDG"
     gene            7979..8329
                     /locus_tag="VF097_10750"
     CDS             7979..8329
                     /locus_tag="VF097_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263313.1"
                     /translation="MAEKRASRSITVVTVILTLGLLWIATPGTAQETYEPWVTDFGRE
                     APAPAYTPWVTDFGGGGEVTDPQPVLDADQEGAPTAAFDWSDVLLGALAATLVWAGIA
                     MAAVLFRSRRRVAA"
     gene            complement(8336..8605)
                     /locus_tag="VF097_10755"
     CDS             complement(8336..8605)
                     /locus_tag="VF097_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HEX6263314.1"
                     /translation="MISVKLPTLLRGHAGGQPEVQSKGGSLGELLENLERRYPGLTDG
                     LRDGRGLRRHVNVYVNGEDVRYTGGLETAVKDGDEVSILPAVAGG"
     gene            complement(8641..9462)
                     /gene="moeB"
                     /locus_tag="VF097_10760"
                     /pseudo
     CDS             complement(8641..9462)
                     /gene="moeB"
                     /locus_tag="VF097_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897946.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
     gene            complement(9459..9890)
                     /locus_tag="VF097_10765"
     CDS             complement(9459..9890)
                     /locus_tag="VF097_10765"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEX6263315.1"
                     /translation="MTIEIPRDRYEEMLAHLRAGGPNEACGVLAGLDGKVAAVYPIRN
                     AEESPVAYRFDEREQLEVFRAVEERGWELLAFFHSHTHTEAYPSPTDVRQAHWEDPDG
                     HPLPAYPGTRYVIVSLMEEVPHLRAFAFEGGAPVEEEVRVV"
     gene            complement(9930..10973)
                     /gene="selD"
                     /locus_tag="VF097_10770"
     CDS             complement(9930..10973)
                     /gene="selD"
                     /locus_tag="VF097_10770"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007786565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(10926..10928),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEX6263316.1"
                     /translation="MRDEVRLTRYSHGAGUACKLGPSDLSQVLGQLGSTEYPAEVVVS
                     QETRDDAAVYRLPDGTGLVQSVDFFTPIVDGARDWGRIAAANALSDLYAMGARPALAL
                     NLVAWPREELSLDLLAEVLRGGSEIVARAGAVVVGGHSIDDPEPKYGMAVTGFVELDR
                     LVRNSTAPPGATLVLTKPLGLGIITTGIKRERAKPEQVAAAIETMTTLNDRAAEAMVA
                     TGAAAATDVTGFGLLGHLHEMLAASSVAAEVDAPSVPFLDGVLELAGQGVVPGGTRRN
                     AAFLDPLVDRDVDEHEWLALVDAQTSGGLLIATESPDGLLGALEARGVAGASIGRVVE
                     GEPGRIRVLGRPA"
     gene            11033..11428
                     /locus_tag="VF097_10775"
     CDS             11033..11428
                     /locus_tag="VF097_10775"
                     /inference="COORDINATES: protein motif:HMM:NF024586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX6263317.1"
                     /translation="MARPARQVALILARDLASQLSTPMFIVDTEGTVIYFNEPAEAIL
                     GKTWAETGELSAEEWGTIFRQRTLEGEPLPREQIPLSIALAEKRPAHTSLRITGMDGV
                     EHTISSTAIPLMAHPDEIVGAVAIFWVTD"
     gene            11428..12288
                     /locus_tag="VF097_10780"
     CDS             11428..12288
                     /locus_tag="VF097_10780"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6263318.1"
                     /translation="MRATIRGCRGSLAAPGPGTVRYGGNTSCVEVQTSDGALIVLDAG
                     TGIRLLGLELEESCPPVIHLMLTHLHLDHLEGLGFFAPLYIPRIEIHLWGPPSPVHSL
                     RDRVERYLSPPLFPVELSDIPSRVMFHDVPEEPWEIGGATISAAPLKHPGPTVGYRIE
                     EEGRSIVYMPDHEPALGSDLRRVPPSWISGYDLAEGADVLMHDAQYTADEYAERLGWG
                     HSALEHVVDFALVTKVERLVMTHHDPNHDDDQLERMLSEAQGMWGEGPGDLVLAREGM
                     TIVPGEDISP"
     gene            12353..12913
                     /locus_tag="VF097_10785"
     CDS             12353..12913
                     /locus_tag="VF097_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263319.1"
                     /translation="MANVFDRAKEAVIGVVKGAGDIVNAVRSTVTDVVVKTLKEAGEI
                     TGTALGVVTDAVRGAIRGTKEAGVEATDSARAIVSGAVEAVAQVGGDVVTGARNAVRG
                     AIQGAAEVGGDVAAAAVGAVEGAVETAGKVGQDVMSVGKAAIETAVETAGTVASGSVD
                     AVKEGLRGAAAVPREVVESALGKDES"
     gene            12955..14079
                     /locus_tag="VF097_10790"
     CDS             12955..14079
                     /locus_tag="VF097_10790"
                     /inference="COORDINATES: protein motif:HMM:NF019522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEX6263320.1"
                     /translation="MLGTVAAAIAAATVLGIIALRPRGEPDANLRELGIASDVYGAAI
                     ESVEIGPCPGTEEAGGVRCRLIGARLLQGPDEGQEVTISLSDTPSTPSFEVGEEIVLA
                     HTPEAQEGFRYQLADRQRRPVLWILFALFVVAVVVLGGIRGMAALAGLAASVVLLILF
                     VLPSILDGRSPLAVAVVGASAIAFLALYLAHGFTLMTTVALLGTVASLALTTVLAELF
                     VRLARLTGLATEEATIVQVGAAEIDLAGLILGGVVIGALGAIDDMTVTQASTVWELHG
                     ANPRLGRRGLFRSGMRVGRDHVASTVNTLLLAYAGASIPLLLLFVLSEQSLGTVANSE
                     VVATEIVRTLVGSIGLVASVPITTWLASWVMGASPAPEGA"
     gene            14076..>14704
                     /locus_tag="VF097_10795"
     CDS             14076..>14704
                     /locus_tag="VF097_10795"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEX6263321.1"
                     /translation="MSELRGFDLWGVPFDGGATLGWPGARYAPDRIRRALSWMRMREE
                     DGLVRAVDIGEDLPAPDVADRGDVDVVPHDLIATIDAVSPAVARSVREGRVPLVVGGE
                     DSIFFPSVRGFHDAVEGSVGVTHFDAHFDLLDESRRQGRFSHSSGMRRSLELDRVAAD
                     RCAQVGTRTFNFPSSAAFVEESGLLQIPAGEVHEQGVARVVERILERVGG"
BASE COUNT         2045 a         5358 c         5164 g         2137 t
ORIGIN      
        1 acagcacggc ggccgcgccg gtccgcggct gtcggacggc ccggagagcg agggcgacac
       61 cctcctcgag ccggagtgat cgcttgctca cgcggaacgc ctccctgagc ccaccccgag
      121 tgttatcggc acggctcacg ctccccccga ccgcctgccg aggcggcgcg tcatccgcgg
      181 acggcccatc ctatcgcctc gcggagcggt gtgagcagga cgtcgaactc cgccgcggcg
      241 tcgggggcgt cggcgcgcgc gtccagcgcc agcaggtcca gatagtccgg agcgaccggc
      301 cctccgaggg cgcgggacgc gcgcgccccc ggcgctaggt gccgcggccg cctcctcctc
      361 ccggccacga tccggacgag ctcgtccgcc gtgacgacat cgggcccctc gaccccgagg
      421 gtcgtcgaga tcgaccccgc ccggtcgtcg gcggccgcca ggatcgcggc cacgtcggcc
      481 gacgccaccg gcgccagcga gaccgagccg tccccgacca ccgcggccgg tctccgcgcg
      541 gccagtgcgg ccacgtcgcg gaaccagggg cttgcgggcg ccacgagccg ggtggcccgc
      601 acgatcgcgt gctccaggtc tgacttgcgc acgagcgcct ccgccctccc cttcgaccgc
      661 aggtacgcgt tgggatcctc gggatccgcc cccgaggtcg acagcaggag gatccgctcg
      721 gcccccgccc ggcgggcgcc ctcgaccgtg cgccgcacgg tctcgaggtt ggcctcctgg
      781 tacgcctggg cgctccgcat ccggatcccc ccggccaggt ggcacaccgt gtgggcgtcg
      841 tggaagagca tctccagcgc gtcggcgtcc ggagctcggg ccaccgcgac cttcgccccc
      901 agggcccgga ggccctcggc ctgggtcggg ctgcggacga tcgcccggac ctcgggggtc
      961 cgctccacga gcagcggcac gagggcgcgg cccaccgagc tcgaggcgcc gaccacgacg
     1021 accggcacgg cctaccagat ctcttcgagg aggtcggagg cgcggagctt ccccagcgcc
     1081 tgccgctcga ccttgcgcac cgtctcggcc gagaccccca ccagctcggc gacctctcga
     1141 aggctcatgg cccgaccgtt ccccaggccg tagcggaggg agacgacctc ctgctccatc
     1201 ggctcgaggg tggtccccac ggcctgcgtg atctcctcgc ggagcagcgt gtcctccacg
     1261 gcgtcgaggg ggctctccag ctcgacctgg gccacgagat cgcccagctc ggcgtcctcc
     1321 cccaccggct cctgcagcga ccggggctcc ttccgcaccg ccgcctccag ctcctccagc
     1381 atctcgaccg ggacgccggc ggcccgtgac agctcgtcgg gcgtggggtc cctccccgtc
     1441 tgctgcatca gccgctcacg ggtcttccgg atcttgcgga tccgctcgtg gacgtggacg
     1501 gggatcctga tctggcgccc gcggtcggcg atggcccgcg tgatcgcctg ccggatccac
     1561 caggtcgcgt aggtggagaa cttgaaacct cgccgccagt cgaagagctc gatcgcgcgc
     1621 atcaggccga tgttcccgtc ctggatcagg tccagcaggg ggagcccctg gccctggtac
     1681 ttcttggcga tcgagaccac gaggcgcagg ttggccagga tgaaccgctt cctcgaccgc
     1741 tcggcctcct tgatgtccgc ggcgagcgtc gccttcgtct tctcggagcg gatccggccg
     1801 gaggcgaggc gctcctccgc ctccttgccg tgctccaggg acatggcgag ctcaacctcc
     1861 tcctccttcg tgaggagcgg ctcgcgggcc gccgcctcga ggtagaggcg gaggtcgtcg
     1921 acgtcgccgg gatggagatc ctgcctcgcc atgcgcctct gggccaatcc tagcgccggc
     1981 cgaggccgga ggtctcactc ccggtcgggg cccccagtgc tcacccgcat ggccttctgg
     2041 tacacgagcg tgccgcccag gtgctgaccc acgatcagga cgaggtaacc cacggcctcg
     2101 atcacgatcc acgagatctc ggcctgcgag gcggtccggt cgggccaccg caggacgaac
     2161 gcgacgatga agaacgccgc agccagaagc tggaacacca tgtggcgcgt ggccagccgc
     2221 cgcttggacg accccttcac catgcccagc cagtcgatga ggcccagggg ggccgcgacc
     2281 agggtgccga ggagccccaa gagcagcatg taggtcgagg ccagcaccag cccctcgttc
     2341 ggggtgatgc ggctcatcac gtcgaagacg atcacgccta ggtagaaggc gacggggaag
     2401 tgcaccacca tcgagtgcgt gggcacgccc agcggcttgc cctgcaccca ctccttcagc
     2461 ttccaggggc gcccctcggg agcccgcatc tggatcagcg ccatcggtcc tcctccctcc
     2521 ggaggccccg gccggcggcc ggggcctccg accaggccga gtctaggcgt tcttgtaggt
     2581 ctcctgcagg ttggacgcgg cctcgtcggc cgagatctcc gcggcctcgg cggccgcctg
     2641 caggtcgttc agggtgatgt ggcggccgtc gccgtggtcg gcgttcggct caccgcatcc
     2701 gcaggtgagg cacatggcgc tcctccttcc ggtcaaatgg ccggccgggc cggccgtccc
     2761 acgatcccta aaactaataa cgcttgtcga tacgaaacgc aagcccctca tcgcagcgct
     2821 tgccgcagcg cccggagggc cctcccccgg tggctgatgc ggtccttctc ctcgggcgag
     2881 agctcggcca tcgtccggtc ccagcccgcg ggctcgaaga tcgggtcgta gccgaaccct
     2941 ccggagccgg agggctcgtc gcggagcagg ccctcgcagt cgccctcggc ccagacctcc
     3001 ccgccgtcgg gccaggcgat gacggccacg gcgcggtagc gggcggtgcg cccgggcccc
     3061 ggtacgcccg cgacggcgcg cagcagctct cggaggttgc gcgcgtcgtc cgcgccctcg
     3121 ccggcgaagc gggcggagcg gggcccgggc gcaccgccga gcgcgtccac ctcgatcccg
     3181 gaatcgtcgg cgagcgcggg gagccccgtg gcctcggcga cctcgtgggc cttccgcttc
     3241 gcgttctgga ggtacgtgcg gccggtctcg tcgacctccg gccagtcggc atccgtcacc
     3301 cactcgaccg gccaatcgct cgcgatcgcc tggatctcgc ggatcttgcc cgggttgcgg
     3361 gatgcgatta cgagccggtc gggcatcgcc cggctcacga cccgagcgcc tcccgctgga
     3421 cctccgtgag ccgggcgatg ccctcctcgg ccagcgtgag catctcctcg agctggtcgc
     3481 gcgagaaggg atcgccctcc gcggtcccct gcacctcgac cagcttcccc gagcccgtca
     3541 tcaccacgtt gaagtccacc tcggcctcgg cgtcctcgtc gtagcagaga tcgaggagcg
     3601 gcaccccccc gacgatcccc gtgctcacgg cggccaccga gtcggtgagg acgcccgagc
     3661 gagccagccc ctgctcctcg aggctccgga gagccagagc cagggcgacg tacccaccgg
     3721 tgatcgaggc cgtccgggtc cccgcgtcgg cctgcagcac gtcgcagtcc accgtgatcg
     3781 agccctcccc cagccgctct aggtcggtca cggcccgcag actccgcccg atcagccgct
     3841 ggatctcctg ggtcctcccc gaggggcgcc cccggttcac ctcccgaggg gtccgctcct
     3901 tggtcgagcg cggcagcatc gagtactcgg ccgtcaccca tcccttcccg gtcccgtcga
     3961 gccagcgcgg cacccgcggc tcccacgtgg ccgcgcacag gactcgggtt ccccccatct
     4021 ccaggaggac ggatccctcg gcgaacgcca gatacccggt ctcgcagcgg atggggcgga
     4081 gctcccgcgg cccgcggccg tcgggtctgc tcacgcgccg gcctcccagc ggacgtccgg
     4141 gcgcgccacg tccaccgcgc cctcgaacgc ctcggaggcc ttctcgcggg agtggtcggg
     4201 gtcgaagatg ggctggatgt gcgccagcat cagccggccg gcgtcggcct tcgccgcctg
     4261 ctcgccggcc tccttcgcgg tcatgtggag atcgggcggg tagtccttcc cgtcctccag
     4321 ccaggtggcc tcggcgacca gcagatccac ccccctcgcc agatccacga gctcgtcggt
     4381 aggcccggta tcggcggaat acgccaacgc caccccgtcg gcctcgtagc gaacccccag
     4441 cgtgggcacc ggatgggcca tgcgggccgt gctcaccctg aacggcccga gctcgaggcc
     4501 cgctccaggc tcgatctcgt ggagctggaa ggagtcgggg aattcctccc acccggcctg
     4561 ggacagcatg catcggatcc gctcctctgt cccggtgggc acgacgagcg ggatcgaagg
     4621 ggccggggtc gcggccccgt agcgtcgcgc gtagaagtac gggaagaggt ccacgaaatg
     4681 gtcggcgtgg acgtgcgaga cgatcaccgc gtcgacgtcg agcagtccca ggtgccgctg
     4741 gaggtgcgcc atcgtccccg tgcccatgtc ctgccagacc acgaacccgt cgtggctcac
     4801 gatgtagccg ctcgcggccc cgtccgcgcg aggccaggtc ccgtcggagc cgaggacggt
     4861 gagctccatc agccgagggg ctctctctgg acgctggcca gcgcgacgtc gtccatctcg
     4921 ggtcccagga agaccctccc cagctcgcgg aagcgatcgg gatccccgga gcagaggaac
     4981 tcgtgccgcg ggaggccctg ttcctcccgc aggaggtcgt cgcggaccag cacggcgtag
     5041 acgtcgcgcg cggtctcctc ggcggacgag atcagcacga cgtccgggcc catcaccatc
     5101 cggatcatcc cggcgagcag ggggtaatgg gtgcagccga ggatgagcgt gtcgacgcct
     5161 gcatcccgga ccggttcgag gtaaccccgc gccgcctccc ggagagctcc gctcgtcgtg
     5221 tcgcccccct ccacgaaggg gacgaacgac gggcaggccc gggccacgag ctccgcgtcg
     5281 atcccgtgat ggtcggccgc ccggtggtag gccccgctga cgatcgtggc ctcggtgccg
     5341 atcacgccga tccgcccgct ccgggtgacc ctggccgcgg cgcgcacgcc cgggtcgatc
     5401 acccccacca ccgggacacc ggcctcggcg gtcacgtcct cgatggcggc gacctcgacc
     5461 gagttgcacg ccaccacgag catcttcacg tcccgatgga ccaggtacgc agcgatctcg
     5521 cgcgagaagg cccggatctc gtccagagga cgggggccgt agggcgcgcg ggccgtgtcg
     5581 ccgtagtaga cgagcggctc tcgcgggagg agatcgagca ccgagcgagc cacggtcagc
     5641 ccgcccacac ccgaatcgaa catcccgatc gctcgcgcgt cgcctaccac cagaccgtct
     5701 cctccacccg cttctccgcc tccgacgcgt cgccggtcca ggcgccagtc gacaggtact
     5761 tccatccgcc gtcgggcagg acgcatacga tatcgccttc cgagacccgt tccccaacct
     5821 tccgggcgac gtggagtacg gcgcccgagg agatcccggc gaagatcccc tcctgctccg
     5881 cgagcatccg ggtcgctcgg agggcgtcgc cggcagccac caggtgcttc ccgtcgagca
     5941 gcgactcttc gaggatcggt gggacgaagc cctcctcgag cgaccgcagg ccgtagaccg
     6001 agtcccccag ctccggctcg gccgcgatca cctgcacgct cgggtcgtgc tccttcagac
     6061 ggcggcccac gcccatcagc gtccccccgg tcccgagccc cgcgacgaag tgggtcgcct
     6121 cgggaaggtc ccggacgatc tcggccccgg ttccctcgta gtgcgctcga gggttcgcgg
     6181 ggttgccgta ctggaacggc atgaagtagc gggggtcctc ggccatgcgc ctcgccacct
     6241 cgatcgagcc gttggtcccg gcgtccgctg gcgagaagac gatctcggcc ccgtagagct
     6301 cgaggagctg gacccgctcg gggctggcgt tctccggcat caccaccatc aggccgtacc
     6361 ccttccggcg ggccaccatc gccagcgcga tcccggtgtt cccggaggtg ggctcgagga
     6421 tcacccggtc gggggtgagc tcgccgctcg tctcggcggc ctcgaccatg gccttcgcga
     6481 tgcggtcctt cagggacccc gtggggttct ggccctcgag cttggcccag agccggacct
     6541 ccgacttggg tgcgaggcgc tggatcccca ccagcggggt gttcccgatc gcctcgagga
     6601 tgtcgctcgc ccgcatccgc ctagtatccc agggccctcc cctcgctgag cgatggcgcc
     6661 gcatcctccg cgggtgcgac gatgtaggcg gaacgtggcc gccgttcgtt catggggcga
     6721 catggtcgac ggaacgacgg cgacgagagc agtggctgcg ttggaggtcg cccccgtgga
     6781 tgaagccggc gtcgcggcgc gggtctcctt cgaggccttc taccgggagc aacacgcacg
     6841 cctctaccgg gccctctgcc tcgtgacgag gaaccggcac gaggcggagg acatcgccca
     6901 ggacgccttc ctcaagctgt acgagcgctg ggagcgcgtc gcgacgctcg aggatccggg
     6961 cggctatctg taccgaacgg ccatgaacgg cttccgctcg cgataccgga gagccgctct
     7021 ggcgctgaag cgaggcctcg gcgtcgtcgc ggccgacgac gggatcgatg ccctggaggc
     7081 cgacgactgg gtgatcaggg ccttggcgcc gctcagccgc cgccagcgag cggcggtggt
     7141 cctgaccgac ctgctggggt actcgtccgt cgaggccggt cggctgctcg ccctgagccc
     7201 ctccaccgtt cgcatgcacg tgtcgagggc gcatgcggcg cttcgcgatc gaatgggtga
     7261 tgagcgatga acgtgtacgg cgatctcctc gagcgtgccg gccgacgctt tccaggcccg
     7321 agcgaccagc tcgacgacct ctacgagcga tggagacggc ggcagcgaaa ccggcgggtg
     7381 gttgccggtg tgacgggcct cgtgattttc gccgtgacgg ccgtcacgct cggatccagg
     7441 ttgcggatcg gagacgacgg gacgcgtccc ggggggtctt cggatcgagc cgtctcggcg
     7501 atcgtgctgg gcgcccggga cgtgcagcgc ggatgggagc tgggagagac gcgcctgggc
     7561 cgggccagcg tgacgctgat cgcgcgcggg gacacgcagg tgctgctgca gcgcctgccc
     7621 ctcatcgacg gcgtcagccg gcggttccga agcgaggatc cgaggccgtt cgcggtcctc
     7681 aacgcgttcg tgctccggtt cggggacgag gccgcggcgc agggaggtta cgcgctgctg
     7741 gagctcgagt tccgacgggg ggacggatcc gccacggagc tccccggtcc tctgctggga
     7801 gtcgagtcga tcgccctcga gcagccgcag gaccgagcgt accgggcgcc cgcgacgatc
     7861 tatctgtggc gccgagggga tctcgtgatg ggactgatgg ccatcggatc gttgcaggaa
     7921 cgtcagttgg aggaactggc tcggacgatg gacgcccggg cagagaggga ggatggagat
     7981 ggctgagaag cgagcctcac gatcgatcac cgtcgtcacg gtgatcctga ccttgggtct
     8041 cctctggatc gcgacgcccg gaacggcgca ggagacgtac gagccgtggg tcacggactt
     8101 cgggagggag gcacccgccc ccgcctacac gccctgggtc accgacttcg gagggggcgg
     8161 cgaggtgacg gatccccagc ccgtccttga cgcggatcag gaaggggctc ccactgctgc
     8221 gttcgactgg agcgatgtcc tcctgggggc actcgcggcc accctcgtgt gggcggggat
     8281 cgcgatggcc gcggtcctgt tccgcagccg gcggcgggtc gcggcctgag cgggctcagc
     8341 cgccggcgac ggcgggaagg atggagacct cgtctccatc cttcaccgcc gtctcgaggc
     8401 ctcccgtgta ccggacgtcc tccccgttga cgtagacgtt gacgtgtcgc cggagacccc
     8461 ggccgtcccg caggccgtcc gtcaggcccg ggtaccggcg ctccaggttc tcaagcagct
     8521 cgcccaggct cccgcccttg ctctgcacct ccggctgacc ccccgcgtgg cctcgcagga
     8581 gggtagggag tttcacgctg atcacgaggc cacctccggc gctcggtcgg cctcgggcgg
     8641 cgattcgccc ggcgtcagac cgcagaacgc ctcgtagtcg atcagctcgg tcaccatcgg
     8701 ctcctcgccg cacaccgggc aggcggggtc ccaccggagg ttcatcgtcc ggaactccat
     8761 cgtgagagcg tccaggagga ggagccgtcc cgccaggggc tcgccgatcc ccgtgacgag
     8821 cttgatcgcc tcggtcgcct ggatcgaccc cacgatcccc ggcagcacgc cgagcacccc
     8881 ggcctcggcg cacgagggca cggtcccggg cggcgggggc tccgggaaga ggcatcggta
     8941 gcaggggtgc ccgggcccgg gcaggaacac gctggcctgc ccctcgaact ggtagatcga
     9001 gccgtaaacc agcggcttcc ccaggatctg ggcagcgtcg ttcgccaggt accgcacggg
     9061 gaagttgtcg gtcccatcca cgatcacgtc gtagggctcg aagatctcga acgcgttccg
     9121 cgaggtgagc ggggtgtcgt gggccacgac ctcgatcgtg gggttgaggt cgagcaggcg
     9181 ctccctcccg gactcgacct tgggccggcc cacatcgtcg gtgtcgtgca ggagctgccg
     9241 ctggaggttc gtcaggtcca ccttgtcgaa gtcgaggagg ccgatccgcc ccaccccggc
     9301 cgcggcgagg tacatcgcca cgggggaccc caggccgccg gccccgatca cgagcaccga
     9361 gccgtcggcg agcttcccct gcccctcggc cccgaggtgc ggcaggaaga tgtgccgtgc
     9421 gtacctcagg acctgctcct ccgtgaaccg gggctccctc atacgacccg gacctcttcc
     9481 tcgaccggtg ccccgccctc gaaggcgaac gcgcggaggt ggggcacctc ctccatcagc
     9541 gagacgatga cgtagcgggt tcctgggtac gccgggaggg ggtggccgtc cgggtcctcc
     9601 cagtgcgcct gacggacgtc cgtgggcgac gggtaggcct ccgtgtgcgt gtgcgagtgg
     9661 aagaacgcca ggagctccca gccacgctcc tcgaccgccc ggaagacctc gagctgctcc
     9721 cgctcgtcga accggtaggc gaccgggctc tcctcggcgt tgcgtatcgg gtagacggcg
     9781 gccaccttgc cgtccaggcc ggccagcacg ccgcacgcct cgttgggccc gccggcgcgg
     9841 aggtgcgcca gcatctcctc gtaccggtcc cgcgggatct cgatcgtcac gccgttaatc
     9901 ctagcaacag ggtcggattc accgtccggt cacgccgggc ggcccaggac gcggatccgg
     9961 cccggctccc cctccacgac cctcccgatc gaggcgcccg cgacgccccg cgcctcgaga
    10021 gcgccgagga ggccgtcggg gctctccgtg gcgatcagca ggccgccaga ggtctgggca
    10081 tccacgagcg ccagccattc gtgctcgtcg acgtctcgat ccacgagcgg gtcgaggaag
    10141 gccgcgtttc gccgggtgcc gccggggacg acgccctggc ccgccagctc gaggaccccg
    10201 tccaggaagg gcaccgacgg cgcgtcgacc tcggcggcca ccgacgaagc cgcgagcatc
    10261 tcgtgcaggt gccccaggag cccgaacccg gtcacgtcgg tggccgccgc ggcccccgtc
    10321 gcgaccatcg cctccgccgc gcggtcgttc agggtggtca tcgtctcgat cgcagccgcg
    10381 acctgctcgg gcttcgcccg ctcgcgcttg atcccggtcg tgatgatccc cagacccagc
    10441 ggcttggtca ggacgagcgt ggcgcccggc ggcgccgtgc tgttccggac gaggcggtcg
    10501 agctccacga accccgtgac ggccatcccg tacttcggct ccgggtcgtc gatcgagtgc
    10561 ccgccgacca cgacggcgcc cgcccgggcc acgatctcgg agccgccccg gagcacctcc
    10621 gccagcagat cgagcgacag ctcctcccgc ggccacgcca cgaggttcag cgccagggcg
    10681 gggcgggccc ccatcgcgta gaggtccgag agggcgttgg ccgcggcgat ccgcccccag
    10741 tctcgggcgc cgtccacgat gggcgtgaag aagtccacgg actgcacgag ccccgtgccg
    10801 tccggcagac ggtagacggc cgcgtcgtcc cgggtttcct gggagacgac gacctcagcc
    10861 gggtattcgg ttgagccgag ctgccccaga acctgggaca ggtccgaggg accgagcttg
    10921 cacgctcacc cggcgccgtg gctgtatctc gtcaggcgca cctcgtcgcg catgggccga
    10981 ggatacccgc gaggggttga gcgggacccg atccctcgct aggctccccg caatggcacg
    11041 cccagcacga caggtcgctc tgatcctggc tcgggatctc gcctcgcagc tgtccacccc
    11101 catgttcatc gtggacaccg agggcacggt gatctacttc aacgagcccg cggaggcgat
    11161 cctggggaag acctgggccg agacgggcga gctctcggcc gaggagtggg gaacgatctt
    11221 ccggcagcgg acgctggagg gcgagccgct ccccagggag cagatccccc tctcgatcgc
    11281 cctcgccgag aagcgcccgg cgcacacgtc cctgcggatc acggggatgg acggggtcga
    11341 gcacacgatc tcctccacag cgatcccgct catggcccac cccgacgaga tcgtcggcgc
    11401 ggtcgcgatc ttctgggtca ccgactgatg cgggccacga tccgagggtg ccgtggctcc
    11461 ctggcggctc cgggacccgg gaccgtccgc tacgggggca acacctcatg cgtcgaggtc
    11521 caaacctccg acggcgcgct catcgtgctc gacgcgggaa cgggcatccg cctcctcggc
    11581 ctcgagctcg aggagtcgtg cccccccgtg attcacctca tgctgaccca cctgcacctc
    11641 gaccacctcg aggggctcgg cttcttcgcc cccctgtaca tcccccggat cgagatccat
    11701 ctgtggggcc cgccctcccc cgtccatagc cttcgggacc gcgtggagcg gtacctgtcc
    11761 ccgccgctct tccccgtcga gctgagcgac atcccctccc gcgtgatgtt ccacgacgtg
    11821 cccgaggagc cctgggagat cggtggcgcg acgatttccg ccgcccccct gaagcatccc
    11881 ggacccacgg tcgggtaccg gatcgaggag gagggccgct ccatcgtgta catgcccgac
    11941 cacgagcccg cgctcggctc cgacctcagg cgggtgcccc cctcgtggat ctcgggctac
    12001 gacctcgcgg agggcgccga tgtgctgatg cacgacgcgc agtacacggc cgacgagtac
    12061 gccgagcgcc ttgggtgggg gcactccgct ctggagcacg tggtggactt cgccctcgtc
    12121 acgaaggtgg agcgcctggt gatgacgcac cacgatccca accacgacga cgatcagctc
    12181 gagcggatgc tgtccgaggc ccaggggatg tggggcgagg gccccggcga cctcgtcctg
    12241 gcccgcgagg ggatgacgat cgtccccggc gaggacatct cgccctgatc gcttccgggc
    12301 ggggaacact ccggactata gtcaggcaac cgagtcgatc ggaggtgcag tcgtggccaa
    12361 cgtcttcgac cgcgcgaagg aggccgtgat cggcgtcgtc aagggcgcgg gcgacatcgt
    12421 caacgccgtc cgctccaccg tcaccgacgt cgtggtgaag accctgaagg aggccggcga
    12481 gatcacggga accgccctgg gggtggtgac cgatgcggtc cggggcgcga tccggggcac
    12541 gaaagaggcc ggcgtcgagg ccaccgacag cgccagggcg atcgtctcgg gcgctgttga
    12601 ggcggtggcc caggtcggcg gggacgtggt gaccggggcc aggaacgcag tgcgcggcgc
    12661 gatccaggga gccgccgagg tcggcggcga cgtggcggcc gcggcggtag gggccgtcga
    12721 gggcgccgtg gagaccgctg gcaaggtggg tcaggacgtc atgagcgtag ggaaagccgc
    12781 gatcgagacg gcggtcgaga ccgccgggac cgtcgcgtcg gggtcggtgg acgcggtgaa
    12841 ggagggcctc cgaggagcgg cggccgtccc tcgcgaggtc gtggagtccg ccctcgggaa
    12901 ggacgaatcg taggacacag gatcgagggg cggatccccc gggggatccg cctcgtgctc
    12961 gggacggtgg ccgcggcgat cgccgcggcc accgtcctcg ggatcatcgc cctccgaccc
    13021 cggggcgagc ccgacgcgaa cctccgggag ctggggatcg caagcgacgt ctacggcgcg
    13081 gcgatcgaaa gcgtggagat cggtccctgc ccgggcacgg aggaggccgg cggggtccgg
    13141 tgccgcctga tcggcgcccg cctcctgcag ggccccgacg agggtcagga ggtgacgatc
    13201 agcctctccg acacaccctc cacgccctcc ttcgaggtcg gcgaggagat cgtcctcgcc
    13261 catacccccg aggcacagga gggcttccgg taccagctcg ccgaccgcca gcgccgcccg
    13321 gtgctgtgga tcctgttcgc gctcttcgtg gtggcggtgg tggtgctcgg agggatccgt
    13381 gggatggcgg ccctggcagg tctggcggcc agcgtggtgc tgctgatcct cttcgtcctc
    13441 ccctcgatcc tggacggtcg gagcccgctg gcggtggccg tggtcggggc ctcggcgatc
    13501 gccttcctgg ccctgtacct ggcccacggc ttcacgctga tgacgaccgt ggctctgctg
    13561 gggaccgtcg cgagcctcgc cctgaccacc gtgctggccg agctcttcgt ccgactggcg
    13621 cgcctcaccg gcctggccac ggaggaggcg acgatcgtgc aggtgggcgc ggccgagatc
    13681 gacctggcgg gactgatcct cggcggcgtg gtgatcggcg cgctcggggc gatcgacgac
    13741 atgaccgtga cccaggcctc gacggtctgg gagctccacg gcgcgaatcc gcgcctcggc
    13801 aggcgaggcc tgttccgctc gggcatgcgg gtggggcgcg accacgtcgc ctcgacggtc
    13861 aacacgctgc tgctcgccta cgcgggggcc tcgataccgc tcctgctgct cttcgtcctc
    13921 tccgagcagt cgctggggac cgtggcgaac agcgaggtcg tggcaacgga gatcgtccgg
    13981 acgctggtgg gaagcatcgg gctcgtggcc agcgtcccga tcacgacgtg gctggcgtcg
    14041 tgggtgatgg gcgcctcgcc ggcgccggag ggggcgtgag cgagctccgc ggtttcgacc
    14101 tctggggcgt cccgttcgac gggggcgcga cgctgggctg gcccggcgcc cgctacgcgc
    14161 ccgatcggat ccgccgcgcg ctctcatgga tgcgtatgcg cgaagaagac gggctcgtcc
    14221 gggcggtgga catcggcgag gacctccccg cgcccgacgt ggccgaccga ggcgacgtgg
    14281 acgtcgtccc ccacgacctg atagccacga tcgatgcggt gtcgccggcc gtggcgaggt
    14341 cggtgcgcga gggccgcgtc ccgctcgtcg tgggcggcga ggactcgatc ttcttcccgt
    14401 cggtccgagg cttccacgac gcggtggagg gatcggtggg cgtgacccac ttcgacgcgc
    14461 acttcgacct gctggacgag agccggcggc aggggcgctt cagccactcc agcgggatgc
    14521 ggcggagcct ggagctcgac cgggtggccg ccgatcggtg cgcccaggtg ggaacccgga
    14581 ccttcaactt ccccagctcc gccgccttcg tcgaggagtc ggggctcctc cagatcccgg
    14641 cgggggaggt tcatgagcaa ggggtggccc gcgtggtcga gcggatcctg gagcgcgtgg
    14701 gcgg
//
LOCUS       DASUTZ010000253         3586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_353192, whole genome shotgun sequence.
ACCESSION   DASUTZ010000253 DASUTZ010000000
VERSION     DASUTZ010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353192"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1512)
                     /locus_tag="VF097_10800"
     CDS             complement(<1..1512)
                     /locus_tag="VF097_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX6263322.1"
                     /translation="MFERFTDRARRVVVLAQEEARLLNHNYIGTEHILLGLIHEGEGV
                     AAKALESLGISLEAVRQQVEDIIGQGQSAPTGHIPFTPRAKKVLELSLREALQLGHNY
                     IGTEHILLGLIREGEGVAAQVLQKLGADLNRVRQQVIQLLSGYTGKEATAGAPGEASP
                     QGSMVLDQFGRNLTQLAREAKLDPVIGREKEIERVMQVLSRRTKNNPVLIGDPGVGKT
                     AVVEGLAQAITKGEVPETIKDKQIYTLDLGALVAGSRYRGDFEERLKKVLKEIKTRGD
                     IILFIDELHTLVGAGAAEGAIDAASILKPMLARGELQTIGATTRDEYRKHLEKDAALE
                     RRFQPIDVSEPSVAHTIDILKGLRDRYEAHHRVSFTDDALVAAANLSDRYISDRFLPD
                     KAIDLIDEAGSRMRIRRMTAPPDVREFDEKIADVRMRKESSIDAQDFETAARLRDEER
                     QLSEQRAVREQEWKAGELDVLSEVDEEEIAEVLAAWTGIPVYKLTEEETEKLLR"
     gene            complement(1736..2002)
                     /locus_tag="VF097_10805"
     CDS             complement(1736..2002)
                     /locus_tag="VF097_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007587882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEX6263323.1"
                     /translation="MGFKTGMIIGFGVGYVLGTKAGRERYEELRQSWQDLMGNPRVQE
                     AVERGKGLVGTATREGLTAVQGGVEKAAGAVKDRLDKDEDLGTG"
     gene            2131..2364
                     /locus_tag="VF097_10810"
     CDS             2131..2364
                     /locus_tag="VF097_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263324.1"
                     /translation="MGEDRHSHEAAVHAHEHVHVTHYARPDEDVTHLEATHEHEHNHP
                     ALEHLHEAHEDEEKEHLREAHIHDHASPAQSPA"
     gene            2364..>3586
                     /locus_tag="VF097_10815"
     CDS             2364..>3586
                     /locus_tag="VF097_10815"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX6263325.1"
                     /translation="MEAPRLLPEAPLDLDGYLAAGGGEGLRAAVELPPEEVIERVRRS
                     GLRGRGGAGFPTGTKWRSVRDDEHPRKFLAVNGAEGEPGTFKDRWLMRRNPYAVLEGT
                     AIAAYAVGVERAFIGVKAGFEREIERLGQAMGEMEEAGILGPTPIQVVPGPDEYLFGE
                     EKALLEVIEGNPPLPRIVPPYMEGLFRKPGEPNPTVVNNVETLANVPLILREGPEAFR
                     SVGTEESPGTMIFTISGDVRRPGVHELELGSSVYDLVFEVAGGPADGRKVKAVFPGAS
                     STVLSLEQIDTPLDFDSMRRVGSGLGSAGFVVYDDTTCIVRTTLTYSRFLYIESCAQC
                     PACKHGTGQITELLERIDRGEGREQDLETILARAMTVTDAQRCALPTGETLIVQSAVQ
                     QFWEEFAAHLGRACET"
BASE COUNT          505 a         1269 c         1247 g          565 t
ORIGIN      
        1 ccggagcagc ttctcggtct cctcctcggt gagcttgtag acggggatgc ccgtccacgc
       61 ggccaggacc tcggcgatct cctcctcgtc gacctcggag aggacgtcga gctcgccggc
      121 cttccactcc tgctcgcgga ccgcgcgctg ctcggagagc tggcgctcct cgtcgcgcag
      181 cctggccgcc gtctcgaagt cctgagcgtc gatcgaggac tccttccgca tccgaacgtc
      241 ggcgatcttc tcgtcgaact cgcggacgtc gggaggcgcg gtcatccgac ggatccgcat
      301 gcgcgacccg gcctcgtcga tcaggtcgat cgccttgtcg ggcaggaacc ggtccgagat
      361 gtagcggtcg gagaggttcg ccgcggccac cagggcgtcg tcggtgaacg acacgcggtg
      421 gtgggcctcg tagcggtcgc gcaggccctt gaggatgtcg atcgtgtggg ccacgctcgg
      481 ctcgctcacg tcgatcggct ggaaccggcg ctcgagggcc gcgtccttct ccaggtgctt
      541 gcggtactcg tcgcgtgtcg tggcgccgat cgtctggagc tcgccgcggg ccagcatcgg
      601 cttcaggatc gaggccgcgt cgatcgcgcc ctccgccgca ccggcgccca ccagcgtgtg
      661 gagctcgtcg atgaacagga tgatgtcgcc gcgggtcttg atctccttca gcaccttctt
      721 gaggcgctcc tcgaagtcgc cgcggtaccg cgaccccgcc acgagcgccc cgaggtcgag
      781 cgtgtagatc tgcttgtcct tgatcgtctc gggcacctcg cccttcgtga tcgcctgggc
      841 caggccctcg accacggccg tcttgccgac gcccgggtcc ccgatcagca ccgggttgtt
      901 cttggtgcgc cgggacagga cctgcatcac gcgctcgatc tccttctcgc gcccgatgac
      961 cggatcgagc ttcgcctcgc gagccagctg ggtgaggttc cggccgaact ggtcgaggac
     1021 catcgacccc tggggggacg cctcgccggg cgcgccggcg gtggcctcct tgcccgtgta
     1081 gccggacagg agctggatca cctgctggcg gacgcggttg aggtccgccc cgagcttctg
     1141 caggacctgg gcggccacgc cctctccctc ccggatcagc cccaggagga tgtgctcggt
     1201 cccgatgtag ttgtggccga gctgaagcgc ctcgcggagc gagagctcca gcaccttctt
     1261 ggcccgcggc gtgaacggga tgtggcccgt cggcgccgac tgcccctggc cgatgatgtc
     1321 ctccacctgc tggcggacgg cctccaggga gatccccagc gactccagcg ccttggcggc
     1381 cacgccctcg ccctcgtgga tcagccccag gaggatgtgc tcggtcccga tgtagttgtg
     1441 gttcaggagc cgggcttcct cctgggctaa gacgacgacc cggcgggccc tgtcggtgaa
     1501 ccgctcaaac atcagtactc cctctgggcc ggttgtccgg ccctataggg tcccctcgag
     1561 gatccgagga ctccgtccta cccccgaggc gcctggggcg ctcccagctt cggagcgccc
     1621 gatggtgtcg attatacccc cggcctccgg cgtgccctcc gaggtgcctg acgttccatc
     1681 ggcacgcgga gccccacacc gtaggtccaa cgcgggccgg tcgcggggtg ttccctcagc
     1741 cggtgcccag gtcctcgtcc ttgtccaggc ggtccttcac cgcgccggcg gccttctcca
     1801 cgcccccctg gaccgcggtg aggccctcac gcgtcgcggt ccccaccagc ccttttcccc
     1861 gttcgacggc ctcctggact cgggggttcc ccatgaggtc ctgccaggac tggcggagct
     1921 cctcgtatcg ctcgcgcccg gccttggtgc cgagcacgta gccgacgccg aacccgatga
     1981 tcatgccggt cttgaatccc atcgcgccct ccttgtcgac cgcaccattc cctcgcgctc
     2041 acggccacaa acgagccgac ccgacgggac cctaccaggt tcggttaggc tcatgcgggg
     2101 gaggtctccg gccaggaagg aggagacgag atgggcgagg atcggcacag ccacgaggcc
     2161 gctgtgcacg cgcacgagca cgtgcacgtg acccactacg ctcggccgga cgaggacgtc
     2221 acgcacctcg aggccaccca cgagcacgag cacaaccacc ccgcgctcga gcacctccac
     2281 gaggcgcacg aggacgagga gaaggagcac ctgagggagg cgcacatcca cgaccacgcc
     2341 tccccggcac agtccccggc gtgatggagg cgccgcggct cctccccgag gccccgctcg
     2401 acctcgacgg gtacctcgcc gccggcggcg gcgagggcct gcgggccgcc gtggagctcc
     2461 cgcccgagga ggtgatcgag cgggtgcgcc gctcgggcct caggggccgc ggcggcgccg
     2521 gcttccccac cgggacgaag tggcggtcgg tgcgcgacga cgagcacccg cggaagttcc
     2581 tcgcggtgaa cggcgcggag ggcgagccgg ggaccttcaa ggaccgctgg ctgatgcggc
     2641 ggaaccccta cgccgtgctc gaaggcaccg cgatcgcggc ctacgcggtg ggcgtcgagc
     2701 gtgcgttcat cggtgtgaag gcggggttcg aacgtgagat cgagcgcctg ggacaggcga
     2761 tgggcgagat ggaggaggcg gggattctcg ggccgacccc gatccaggtg gtgccgggcc
     2821 cggacgagta cctcttcggg gaggagaagg ccctccttga ggtgatcgag ggcaacccgc
     2881 cgctcccgcg catcgtcccc ccctacatgg agggcctgtt ccggaagccc ggggagccca
     2941 acccgaccgt cgtgaacaac gtggagaccc tggccaacgt cccgctcatc ctgcgggagg
     3001 gaccggaggc gttccgctcc gtcgggacgg aggagtcgcc ggggacgatg atcttcacga
     3061 tctcgggcga cgtgcgccgg ccgggggtgc acgagctcga gctggggagc tccgtctacg
     3121 acctcgtctt cgaggtggcg gggggaccgg cggacggccg gaaggtcaag gcggtgttcc
     3181 caggggcgtc ctcgacggtg ctctctctgg agcagataga caccccgctc gacttcgact
     3241 cgatgcggcg ggtcggatcg ggcctggggt cggccgggtt cgttgtctac gacgacacca
     3301 cgtgcatcgt gcggacgacc ctgacctact cgcggttcct gtacatcgag tcgtgcgccc
     3361 agtgccccgc gtgcaagcac ggcacgggac agatcaccga gctgctggag cggatcgatc
     3421 ggggcgaggg ccgcgaacag gacctggaga ccatcctggc ccgggcgatg acggtgaccg
     3481 acgcccagcg gtgcgcgctc ccgacggggg agacgctgat cgtgcagagc gccgtccagc
     3541 agttctggga ggagttcgcc gctcacctcg gccgggcgtg cgagac
//
LOCUS       DASUTZ010000254        10793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1131472, whole genome shotgun sequence.
ACCESSION   DASUTZ010000254 DASUTZ010000000
VERSION     DASUTZ010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10793
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131472"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..430
                     /gene="sodN"
                     /locus_tag="VF097_10820"
     CDS             14..430
                     /gene="sodN"
                     /locus_tag="VF097_10820"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HEX6263326.1"
                     /translation="MLSRLLRPRRVVRAHCDLPCGVYDPAQARIEAESVQKIQEKYQD
                     AENQKKPSETVEDFKTRCLIIKEQRADLVKHHLWVLWTDYFKPEHLEKYPDLHDLYWR
                     ATKAAGEAKKSVDPKQGQELLDVIGEIDKIFWDTKK"
     gene            complement(479..664)
                     /locus_tag="VF097_10825"
     CDS             complement(479..664)
                     /locus_tag="VF097_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009592746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263327.1"
                     /translation="MAKRMTRAEAGRRGGLSTKRKYGSDFYQEIGRRGGKKGGQTTKK
                     RYGVEFYQKIGKKGGSR"
     gene            775..1845
                     /locus_tag="VF097_10830"
     CDS             775..1845
                     /locus_tag="VF097_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX6263328.1"
                     /translation="MRVEEYLSFMVEAEASDLHFKVGLPAIIRVHGVLHRTHFPPLGD
                     EDTERLALELLPENRVPQFREGKEIDLGYTSPGGTRFRVNVYRQRGHVSVAIRLVRTR
                     IPTFEELMLPEGAQLLAKEPRGLVLVTGPAGTGKTTTIAAMLGWINRHRRVHIITIED
                     PIEVMHEDLQAVVEQRELGVDTDSYAAALRAALRQDPDVIFIGEIRDRDTVEAAVQAA
                     ETGHLVIATLHTLDATETVNRVIDLFPAEIQQQARVALAAGLKGVLSQRLLPRADSEG
                     RVPAVEVLINTGRVAERIVDPEQTFTIPDVMAEGEFYAMQTFDQAIVQLVRDGLVTVE
                     DATHAATNPHDLMLALQAAQLV"
     gene            complement(1811..2203)
                     /locus_tag="VF097_10835"
     CDS             complement(1811..2203)
                     /locus_tag="VF097_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263329.1"
                     /translation="MKRLLGVLMRVWTLPNTLIGLLLGALSFQRPRLDEGVVVFDRGP
                     RGFLWLFGKLSRYSAITFGHVVLSVRAVEGSLRRHERHHVAQYEVLGPLFLPTYLGLF
                     AVKGYRGHPFEVEASEAAKRAGPPGAPA"
     gene            2240..3640
                     /locus_tag="VF097_10840"
     CDS             2240..3640
                     /locus_tag="VF097_10840"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019324693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX6263330.1"
                     /translation="MSDPSQEHVAGLLSLPPEVDELGTRFREAGHELYVVGGSVRDAI
                     LRREIEGRDLDFTTDARPEDVIRILQPWADARYLQGIRFGTVGARKGDLRVEITTFRE
                     EVYPEPDSRRPHVTFARDVKDDLSRRDFTINAMAVRVPDRLFVDPFRGLYDLGHRRLR
                     TPLDPEISFGDDPLRMLRAARFVSVLGVIPEPEVVDAIRRLRDRLEIVSGERIRDELS
                     KLLLGPEVVAGLRLVLETGLADVFLPELPALRLEQDPVHKHKDVLEHTLAVVGRVEPR
                     LPVRLAALLHDIGKPATREITSEGVQFHHHDVVGARMAEERLRALRYPGDVVEEVRRL
                     IEMHLRFHTYGDGWSDGAVRRYVRDAGPLLDDLNMLTRADCTTQNPRKATMLARLQNE
                     LEARIARLAEEENLARIRPPIDGNQVMERLGVEPGPVVGEALDHLLEVRMERGPIEEE
                     EALRLLDEWWDERRTR"
     gene            3616..4092
                     /locus_tag="VF097_10845"
     CDS             3616..4092
                     /locus_tag="VF097_10845"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX6263331.1"
                     /translation="MGRTKDTLMRTAVDVHNFLVEREVPHELVPTQGRLRPPERVAAV
                     LGLEPGQVGRVRLFQGQGLVAALVQADREPSPDLLRAAAGEVAEVDDARATDLTQFLA
                     EATPPVGLPEGTTTILDEVLAGQEVLYFPGGEATSLLKIRPDDIIAATGARVAPLT"
     gene            4089..4520
                     /locus_tag="VF097_10850"
     CDS             4089..4520
                     /locus_tag="VF097_10850"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX6263332.1"
                     /translation="MRTRHEVSAGGVVVRGAGREAEILLASRRVRSGDLVWGLPKGLI
                     EEGESGEDAAVREVREETGWQASLMRHLRDIEYWFVWEGTRVHKKVRFYLMKAEREDP
                     DGRDHEMEEVRWFPLAEAASVAGFDSEREVLLEAAEALGAG"
     gene            4548..6542
                     /locus_tag="VF097_10855"
     CDS             4548..6542
                     /locus_tag="VF097_10855"
                     /inference="COORDINATES: protein motif:HMM:NF039636.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HEX6263333.1"
                     /translation="MTPRILPLLVLLVLLPIPGALAQEEEPSIVELALVRHTPVATPD
                     RPLEVSLSAHYPIGRPMRDLTASLWIYHPATSRSAYHQGLADEPPTAPLLITPYPLRG
                     PLLEGETLEIRIRHDLAELVARGENALYPIKLQLESGGVPLAAVRSALVFVQEKPLVP
                     LNLSLTVVLDPEIRFSPGGVLLDGSLEAAIGEGGSVESLVSALEAEPVRATLALSPLM
                     LEWLDRMSEGYRVRQAEDERSVGADAPEAEAADRMLERIRSLARSAAIEVLALPYASP
                     SIPSLVASGLEEDLRRQISEGRDVVQSLLGVTVSTETLHPPRSELDRDSVAALAEGGI
                     ESLVLSAERVRPLVPPDSIYSPSATVRLDPTMDAIVPDAQVEAYLSPPPEAPTLRAMH
                     LLGELAAHYFEQPSLLRGAALVLGGDNDGLFLRALLRAIRTTGPRSAWLRPVKASQLA
                     QAVPPETRGRLRAPEPETFSPGFVEAVSETRDAIDQFESMTEAPLDLPERLRTLLLAA
                     EARRFALHEGEALGFIRMIRTTLAEEFDKIDAPSGTSVTLTSQGGVIPVTLRSSAEYP
                     VRLRITLRSSRLEFLQGASREVVLDGEALSLTFPVRAQATGRFPVQILLDTPGGRLIS
                     ESEIVVRSTAYNLVALAVTVGAALFLALWWGRRFWRRTTS"
     gene            6506..8185
                     /gene="murJ"
                     /locus_tag="VF097_10860"
     CDS             6506..8185
                     /gene="murJ"
                     /locus_tag="VF097_10860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX6263334.1"
                     /translation="MGKEVLAADDIVTGGPPAEGPPPPGSQPEGLVRSTALMSVGTTL
                     SRVTGFGRVAAMTFAIGVAESRLADVYNVANNMPNIIYELVLGGVLSSVFVPVFVEQI
                     ASRTKDEAWHVARAVMTVTMVLLGTVMVIGIVAAPWIVRLYTFRVEAEAQAAAEALGT
                     LFLQLFMPQIVLYGIGAAATGLLNAHRRFVVPMFAPILNNIVVIASFLVFAWMYASAQ
                     PPTPGNITLAQKLVLSVGTTAGVLAMTVALWPSLRRIGFRWRWALDFRHPAIRKLIRL
                     SGWAVGYVIVNQIGLLIVIALAARDQGAYTAYQAAFIFFQLPHAIFSVSIMTALLPSM
                     SERWVGGDRTGFRRILARGVRASAFIVVPAAAGYMVLARPIVRLLLEHGVTTAESTEL
                     LAGVLTYFALGLFSFSAFMLFLRGFYAMQDTRTPMLVNIFAVSLHTGVNFILFPLMGV
                     EGLALGHAAAYTFAAIVSAVILRRRLGGLEGGSLLAGLGKVVAAAGLTAAASWGAARL
                     AARAFGVDTLTDQIIQVGVGVLAGLIIFIVAALLFRMEELHLLKRAFLRRR"
     gene            8182..8598
                     /locus_tag="VF097_10865"
     CDS             8182..8598
                     /locus_tag="VF097_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263335.1"
                     /translation="MSGRLPFLAERLRDERGILVAALVRLVIFLALLGLVLFETGSVI
                     WSRLSAQDTADAAATTAVTAYRDSRDVRVAEQEARRTVEDKDESAEMTFFHVQTDGSV
                     TVRVRKKAQTLVIQHIGFLEGFTRARGSATIEPPSF"
     gene            8628..10115
                     /locus_tag="VF097_10870"
     CDS             8628..10115
                     /locus_tag="VF097_10870"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX6263336.1"
                     /translation="MKTEHGRKLAGRYVLDSPLASGGMAEVWSGRDEVLGRPVAVKIL
                     HEHLATDDEFLDRFRREAITAARLSHPCVVRVFDTGVDDGVVFIVMELYEGASLADRL
                     RDGPLSPEEAARIARGILDGLAHAHRQKVVHRDVKPANVLLHGAQVKVSDFGIAKAAH
                     GGDLTTTGQLLGTARYLAPEQVDAASVDHRADLYAMGIVLYEMLTGRTPFEAETAIAE
                     ATLRLTEDPRPPGALRGGIPRELEDVVMRALARDPDDRFQSAAEMREALDGAVWTAPP
                     PAPPPSEEEPEPTPSFFRAWMLVPLAAVLLAAAVIVGGLLFGSLELGGPLGVRPRDDP
                     GSPAAPAGGPLEVASVQAHDPEGTGGEHDDEATFAADGDQSTAWTTEGYTRPDLGGIK
                     SGVGLIFDLGSPADVAGLEMATTAPGWTFRVFASGDLAPLVEPASFPRPLANENGETS
                     FTAEETTTLAFPQARGRYVLIWITELTDVEGRFRALIAETEFTGG"
     gene            10108..10668
                     /locus_tag="VF097_10875"
     CDS             10108..10668
                     /locus_tag="VF097_10875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX6263337.1"
                     /translation="MADDRELVRAHLGGDRGAFSTLVERHQRRVYNIAYRMVGPDEAA
                     DATQEVFLTCLRRLSGFRGDSAFTTWLHRVTVNVCLDALRKRSREGPWRDAEGPQPVE
                     RDVADAVVASADVERALALVPEEFRAPLVLHDLQDLPYEEIADILGAPIGTVKSRIHR
                     GRVALARALRGEPPPPRDPSNVQETT"
BASE COUNT         1575 a         3856 c         3721 g         1641 t
ORIGIN      
        1 ggaaggagga ggcatgctca gccgactgct gaggcccagg cgggtcgtcc gcgcgcactg
       61 cgacctcccc tgcggcgtct acgacccggc ccaggccagg atcgaggcgg aatcggtcca
      121 gaagatccag gagaagtacc aggacgcaga gaaccagaag aagccgtccg agacggtgga
      181 ggacttcaag acgcgctgcc tgatcatcaa ggagcagcgt gcggacctcg tcaagcacca
      241 cctgtgggtg ctgtggacgg actacttcaa gccggagcac ctcgagaagt acccggacct
      301 ccacgacctg tactggaggg ccacgaaggc ggccggcgag gcgaagaagt ccgtggatcc
      361 gaagcagggc caggaactgc tggacgtgat cggggagatc gacaagatct tctgggacac
      421 caagaagtga gaagtgcccg cgggccgggg gcttgccccc cggccctgcg ggtgacgcct
      481 accgggagcc gcccttctta ccgatcttct ggtagaactc cacgccgtag cgcttcttcg
      541 tggtctggcc gcccttcttc ccgccgcgcc gaccgatctc ctggtagaag tcgctcccgt
      601 acttcctctt ggtggagagg ccgccacgac ggccggcctc cgctcgcgtc atgcgcttgg
      661 ccaacttcgt cccctccttc tgctcgcagg tgtacgccta caggtacccg cggcttgcct
      721 cggcaaaccc gaccgtcaag ggaggcgggc atcgagccga tgatggaagc ggagatgagg
      781 gtcgaggagt acctctcgtt catggtggag gcggaggcct ccgacctgca cttcaaggtc
      841 gggctgccgg ccatcatccg cgtgcacggc gtgctgcacc gcacacactt ccctccgctg
      901 ggcgacgagg acaccgagcg gctggccctc gagctcctcc cggagaaccg cgtgccgcag
      961 ttccgcgagg ggaaggagat cgacctgggc tacacatccc ccggcgggac caggttccgg
     1021 gtcaacgtct accggcagcg cgggcacgtg agcgtcgcga tccgcctggt gcggacccgg
     1081 atccccacgt tcgaggagct catgctgccc gagggcgcgc agctcctggc caaggagccg
     1141 agagggctcg tgctggtgac gggtcccgcg gggaccggca agacgacgac gatcgccgcc
     1201 atgctcggct ggatcaaccg gcaccgcagg gtccacatca tcacgatcga ggacccgatc
     1261 gaggtcatgc acgaggatct gcaggcggtg gtcgagcagc gcgagctggg ggtggacacc
     1321 gactcctacg ccgcggccct ccgcgcggcc ctccggcagg accccgacgt gatcttcatc
     1381 ggcgagatcc gcgaccgcga cacagtcgaa gcggcggtgc aggcggccga gactggccac
     1441 ctcgtaatcg cgaccctcca caccctcgac gcgacagaga ccgtcaaccg cgtgatcgac
     1501 ctcttccccg ccgagatcca gcagcaggca cgcgtggcgc tggccgcagg gctcaagggc
     1561 gtgctctccc agcgtctcct cccgcgagcg gacagcgagg gacgggtgcc ggcggtcgag
     1621 gtgctgatca acacgggccg cgtggccgag cggatcgtcg acccagagca gaccttcacg
     1681 atccccgacg tgatggccga gggcgagttc tacgcgatgc agaccttcga ccaggccatc
     1741 gtgcagctcg tccgggacgg cctcgtcacc gtcgaggacg cgacgcacgc ggccacgaac
     1801 ccgcacgatc tcatgctggc gctccaggcg gcccagctcg tttagcggcc tccgacgcct
     1861 cgacctcgaa cgggtgaccc cggtagccct tgacggcgaa cagccccagg tacgtcggga
     1921 ggaagagcgg ccccagcacc tcgtactgcg cgacgtggtg acgctcgtgg cggcggaggc
     1981 ttccctcgac ggcccgcacc gagaggacca cgtgcccgaa cgtgatcgcc gagtaccggg
     2041 agagcttccc gaagagccac aggaaccccc gggggccgcg gtcgaagacc accacgccct
     2101 catcgagccg gggacgctgg aacgagagcg cccccagcag cagaccgatc agggtattgg
     2161 gcagcgtcca gacgcgcatc agcacgccga gcagcctctt cacgtggtgg cctcctcccc
     2221 ctagggtatc ctcctcgttg tgtcggaccc ctcccaggag cacgtcgccg gcctgctgag
     2281 cctgcccccc gaggtggacg agctaggaac ccgcttccgg gaggccggtc acgagctgta
     2341 cgtcgtgggc gggtccgtgc gggacgcgat cctgcggcgc gagatcgagg gccgtgacct
     2401 cgacttcacc accgacgccc gccccgagga cgtcatccgg atcctccagc cgtgggctga
     2461 cgcacggtac ctgcagggga tccgcttcgg aaccgtcggg gcgaggaagg gcgacctccg
     2521 cgtcgagatc acgaccttcc gggaggaggt gtatccggag ccggactccc gccggcccca
     2581 cgtcacgttc gcccgggacg tcaaggacga tctctctcgc cgagacttca cgatcaacgc
     2641 catggccgtg cgcgtccccg atcggctctt cgtggatccg ttccgcggcc tctacgatct
     2701 gggccaccgc cgcctgcgga ccccgctcga tcccgagatc tcgttcggcg acgacccgct
     2761 gcggatgctg cgcgccgcga ggttcgtctc ggtcctcggc gtgatcccgg agcccgaggt
     2821 cgtggacgcg atccggcggc ttcgcgaccg cctcgagatc gtctcgggcg aacggatccg
     2881 ggacgagctg tcgaagctgc tgctcgggcc cgaggtcgtg gccggcctcc ggctcgtgct
     2941 tgagacgggg ctcgccgacg tcttcctccc cgagcttccc gcgctccgcc tggagcagga
     3001 ccccgtccac aagcacaagg atgtgctgga gcacacgctg gcggtggtgg ggcgggtgga
     3061 gccgaggctg ccggtccggc tggccgcgct cctgcacgac atcgggaagc ctgccacccg
     3121 cgagatcacg tccgagggcg tgcagttcca ccatcacgac gtagtggggg cacggatggc
     3181 ggaggagcgt ctgcgcgcgc tccgctatcc gggcgacgtg gtcgaagagg tacggcggct
     3241 gatcgagatg cacctgcgct tccacaccta cggcgacggg tggagcgacg gggccgtccg
     3301 gcggtacgtc cgggacgccg ggccactcct cgacgacctc aatatgctga cgagggccga
     3361 ctgcacgacg cagaacccca ggaaggcgac gatgctggcc aggctccaga acgagctcga
     3421 agcccggatc gcccgcctgg cggaggagga gaacctggcc cggatccgtc ccccaatcga
     3481 cgggaaccag gtgatggagc gtctgggcgt cgagcccggg cccgtcgtgg gcgaggcgct
     3541 ggaccacctc ctcgaggtcc ggatggagcg gggtccaatc gaggaggagg aggcgctccg
     3601 gctcctcgac gagtggtggg acgaacgaag gacacgctga tgcgaacggc cgtggacgtc
     3661 cacaactttc tcgtggagcg cgaggtcccc cacgagctcg tgcccacgca gggccgactg
     3721 cggccgcccg agcgcgtcgc cgcggtcctg gggctggagc ctgggcaagt ggggcgcgtc
     3781 cgcctcttcc agggtcaagg gctcgtggcc gcgctggtcc aggccgaccg cgagccgtcg
     3841 cccgacctgc tcagggccgc cgcgggagag gtcgccgagg tcgacgacgc tcgcgccacc
     3901 gacctcacgc agttcctggc cgaggccaca cccccggtgg gcctccctga gggcaccacg
     3961 acgatcctcg acgaagttct cgccgggcag gaggtcctct acttccccgg cggggaggcc
     4021 acttcgctcc tgaagatccg gccggacgac atcatcgccg cgaccggggc gcgcgtggcg
     4081 cccctcacgt gaggacgagg cacgaggtat cggccggggg cgtggtcgtc cgcggcgctg
     4141 gccgggaggc cgagatcctc ctcgcctccc gcagggtccg atccggcgat ctcgtttggg
     4201 gcctgcccaa gggtctgatc gaggagggcg agtcgggcga ggacgccgcg gttcgggagg
     4261 tgcgtgagga gacgggctgg caggcttcgc tcatgcgaca cctccgtgac atcgagtact
     4321 ggttcgtctg ggaggggacc agggtgcaca agaaagtgcg cttctacctt atgaaggcgg
     4381 agagggagga cccggacggg cgcgaccacg agatggaaga ggtccgctgg ttccccctgg
     4441 ccgaggccgc ctccgtggcc gggttcgatt cggagcgaga ggtgctcctg gaggcggccg
     4501 aggcccttgg ggccggctga ccgagccggg catacaatga ccgcgccgtg acgcctcgca
     4561 tcctgcccct gctggtgctg ctcgtccttc tccccatccc cggcgcgctc gcgcaggagg
     4621 aggagccctc gatcgtcgag ctggccctcg tccgccatac cccggtggcc acgcccgatc
     4681 gcccgctcga ggtctcgctg agcgcccact acccaatcgg gaggcccatg cgcgatctga
     4741 cggccagcct gtggatctac catcccgcca cctcacggtc cgcgtaccac caggggctgg
     4801 ccgacgagcc gcccacggcc cccctgctta tcacccccta tccgctgcga ggaccgctcc
     4861 tggaaggcga gaccctggag atccgaatcc gccacgacct cgcggagctc gtggctcggg
     4921 gagagaacgc cctgtacccg atcaagctcc agctcgagtc aggcggcgtc cccctggcgg
     4981 ccgtccgctc agcgctcgtc ttcgtccagg agaagccgct ggtccccctc aacctctccc
     5041 tgaccgtcgt gctcgatccc gagatccgct tctcgccggg gggggtgctc ctggacggct
     5101 cgctggaggc ggcgatcggc gaggggggtt cggtcgaatc gctcgtctcg gcgctggagg
     5161 ccgagcccgt gcgggcgacg ctcgccctct cccctctcat gctcgagtgg ctcgatcgga
     5221 tgagcgaggg gtaccgcgtg aggcaggcgg aggatgagcg ctcggtcggg gccgacgccc
     5281 ccgaggcgga ggctgccgac cgcatgctgg agcggatccg atcgctggcg aggagcgccg
     5341 ccatcgaggt cctcgccctc ccctacgcca gcccctcgat cccctcgctc gtggcatcag
     5401 ggctcgagga ggacctccgc cgccagatca gcgaggggag agacgtggtc cagtccctgc
     5461 tcggcgtcac cgtctcgacc gagaccctcc acccgccccg gtccgagctg gacagggaca
     5521 gcgtcgccgc gctcgcggag ggcggcatcg agtccctcgt gctgagcgcc gaacgcgtcc
     5581 ggcccctcgt cccgccggac tcgatctaca gcccctccgc caccgtgcgc ctcgatccca
     5641 cgatggacgc catcgtcccc gacgcgcagg tcgaggccta tctctctccc ccgcccgagg
     5701 cgcccacgct gcgcgcgatg cacctgctgg gggagctcgc cgcccactac ttcgagcagc
     5761 cgagcctgct gcggggagcg gccctggtcc tgggaggaga caacgatgga ctcttccttc
     5821 gtgccctcct ccgagcgata cgcaccacgg gcccccgctc cgcctggctc cgccccgtga
     5881 aggcctctca gctggcccag gcggtgccgc cggagacccg aggtcgcctc cgcgccccgg
     5941 agcccgagac cttctccccg gggttcgtgg aagccgtgag cgagacccgg gacgccatcg
     6001 accaattcga gtcgatgact gaggcccccc tcgacctgcc cgagcggctc aggacgctgc
     6061 tcctggccgc agaggccagg cgcttcgcgc tccacgaggg ggaggcgctg ggcttcatcc
     6121 ggatgatccg tacgaccctg gccgaggaat tcgacaagat cgacgctccc tcggggacct
     6181 cggtgacgct gacctcccag ggcggggtga tccccgtcac cctccggagc tcggccgagt
     6241 acccggttcg gctccgcatc accctgcgct cctcgcggct cgagttcctc cagggcgcgt
     6301 cccgcgaggt cgtcctggac ggcgaggccc tgtccctcac cttccccgtc cgggcccagg
     6361 ccacgggccg cttccccgtc cagatcctcc tggacacgcc aggcggcagg ctgatcagcg
     6421 agtcggagat cgtggtgcgg tccacggcct acaacctcgt ggccctggcc gtgacggtgg
     6481 gcgcggcgct gttcctcgcc ctctggtggg gaaggaggtt ctggcggcgg acgacatcgt
     6541 gaccgggggc ccgcccgcgg aggggccacc cccgccgggc tcgcagcccg aggggctcgt
     6601 gcggagcacc gccctcatgt ccgtgggaac caccctctct cgggtcaccg gcttcggccg
     6661 ggtcgccgcg atgaccttcg cgatcggtgt ggcagagtcg cgcctggccg acgtctacaa
     6721 cgtcgccaac aacatgccca acatcatcta cgagctggtg ctgggcgggg tcctctcttc
     6781 ggtcttcgtg ccggtattcg tcgagcagat cgcctctcgg acgaaggacg aggcgtggca
     6841 cgtggcgagg gccgtgatga ccgtgacgat ggtcctgctc gggaccgtca tggtgatcgg
     6901 gatcgtggcc gctccctgga tcgtccgcct ctacaccttc cgcgtggagg ccgaggcgca
     6961 ggcggccgcg gaggcgctcg gcacgctgtt cctccagctc ttcatgcccc agatcgtcct
     7021 gtacgggatc ggggcggcgg ccaccggcct gctcaacgcc caccggcggt tcgtggtgcc
     7081 gatgttcgcc cccatcctca acaacatcgt ggtcatcgcc tcgttcctcg tgttcgcctg
     7141 gatgtacgcg agcgcccagc ctccaacccc cggcaacatc accctggctc agaagctggt
     7201 gctcagcgtg gggaccacgg ccggcgtgct ggccatgacc gtcgctctct ggccctcgct
     7261 gcggcggatc ggcttccggt ggcggtgggc gctcgacttc cgccaccccg cgatccggaa
     7321 gctgatccgg ctatcgggct gggcggtggg ctatgtgatc gtgaatcaga tcggcctgct
     7381 gatcgtgatc gctctcgcgg cccgggatca gggcgcctac accgcctacc aggctgcttt
     7441 catcttcttc cagctgccgc atgcgatctt ctcggtctcg atcatgacgg ccctcctccc
     7501 gtccatgtcg gagcgctggg tcggtggcga ccggaccggc ttccgccgca tcctggcccg
     7561 cggcgtccgg gcctccgcgt tcatcgtcgt gcccgctgcc gccgggtaca tggtgctggc
     7621 gcgtccgatc gtccggctgc tgctcgaaca cggcgtcacc acggcggagt cgaccgagct
     7681 gctggccggc gtgctcacgt acttcgccct cggcctgttc tcgttctcgg cgttcatgct
     7741 gttcctgagg ggcttctacg cgatgcagga cacacggaca ccgatgctcg tcaacatctt
     7801 cgcggtctcg ctgcacacgg gggtcaactt catcctcttc ccgctcatgg gcgtggaggg
     7861 gctggccctg gggcacgccg cggcctacac gttcgcggcg atcgtctccg cggtcatcct
     7921 gcgcaggcgg ctgggcggtc tggagggcgg ttccctcctg gcggggctgg ggaaggtggt
     7981 cgccgccgcg gggctcaccg cggccgcctc gtggggagcg gcccgcctcg cggcccgcgc
     8041 cttcggcgtc gacacactga cggatcagat catccaagtg ggggtgggcg tcctggcggg
     8101 gcttataatc ttcatcgttg cggccctgct cttccggatg gaggagttgc acctgttgaa
     8161 gagagcgttc ctgcgccggc gatgagcggc cgcctcccct tcctcgccga gagactgcgc
     8221 gacgagcgcg gcatcctggt cgcagccctg gtgcggctcg tgatcttcct cgccttgctc
     8281 gggctggtct tgttcgagac ggggtccgtg atctggtcga ggctctccgc ccaggacact
     8341 gccgacgccg ccgccaccac cgcggtcacc gcttaccggg acagcaggga cgtccgggtg
     8401 gccgagcagg aggctcgtcg gacggtcgag gacaaggacg agtccgccga gatgaccttc
     8461 ttccatgttc agaccgacgg cagcgtcacg gtccgagtcc ggaagaaagc ccagacgctg
     8521 gtaatccagc acatcggatt cctggagggc ttcaccaggg cccgggggtc cgccacgatc
     8581 gagccgccct cgttctgatc tcgagggcac catctacact gagctcgatg aagactgagc
     8641 acgggcggaa gctcgcgggc cgctacgtcc tcgactcgcc cctcgcctcg ggaggcatgg
     8701 cggaggtctg gagcgggcgc gacgaggtcc tgggccgtcc ggtcgccgtc aagatcctcc
     8761 acgagcacct cgccaccgac gatgagttcc tcgaccggtt ccggcgcgag gcgatcacgg
     8821 cggcccgact ctcccatccc tgcgtcgtgc gggtgttcga caccggcgtg gacgatgggg
     8881 tggtcttcat cgtgatggag ctgtacgagg gcgcctcgct ggccgaccgg cttcgcgacg
     8941 gccccttgtc gccggaggag gcggcgcgca tcgcgcgcgg catcctggac ggcctcgccc
     9001 acgcccacag gcagaaggtc gtccaccgcg acgtcaaacc cgccaacgtc ctcctccacg
     9061 gagcccaggt gaaggtgagc gacttcggga tcgcgaaggc ggcccacggc ggcgacctga
     9121 ccaccacggg gcagctgctc ggcaccgccc gctacctcgc tccggagcag gtggacgccg
     9181 cctcggtcga ccatcgcgcg gatctctacg ccatgggcat cgtcctgtac gagatgctga
     9241 ccgggcggac gcctttcgag gcggagaccg ccatcgcgga ggccaccctg cgcctgacag
     9301 aggatccccg cccccccggc gcgctccgcg ggggcatccc tcgggagctg gaggacgtcg
     9361 tgatgcgagc cctggcccgg gacccggacg atcggttcca gtcggcggcg gagatgcgcg
     9421 aggcgctcga cggggcggtc tggaccgctc ccccgccggc ccctcccccg tccgaggagg
     9481 agccggagcc gaccccctcg ttcttcaggg cctggatgct cgtgccgctg gcggccgtcc
     9541 tgctggcggc ggcggtgatc gtgggcggtc tgctcttcgg gagcttggag ctcggcggtc
     9601 ccctcggcgt ccgcccgcgc gacgacccgg gctccccggc cgcgccggcc ggcggcccgc
     9661 tggaggtggc gtcggtccag gcccacgatc ccgaggggac gggtggcgag cacgacgacg
     9721 aggcgacctt tgccgccgac ggcgaccaat cgaccgcctg gacgaccgag ggctacacga
     9781 ggccggatct gggaggtatc aagtcgggcg tcggcctcat cttcgatctc ggatcgcccg
     9841 cggacgtggc cggcctcgag atggccacga ccgcgccggg atggacgttc cgggtcttcg
     9901 cctccggcga cctcgccccg ctggtggagc cggcgtcctt ccctcggccc ctggccaacg
     9961 agaacggtga gaccagcttc accgccgagg agacgacgac actcgccttc ccccaggccc
    10021 gtgggcgata cgtccttatc tggatcaccg agctgaccga cgtcgagggc cggttccggg
    10081 ccctgatcgc cgagaccgag ttcacgggtg gctgacgacc gagagctcgt ccgggcccac
    10141 ctggggggcg accggggagc cttctccacg ctcgtggagc gacatcagcg ccgcgtctac
    10201 aacatcgcgt accggatggt cggaccggac gaggcggccg acgccaccca ggaggtgttc
    10261 ctgacgtgcc tgcggaggct ctcaggcttc cgaggggact cggccttcac cacctggctg
    10321 caccgggtga ccgtgaacgt ctgcctcgac gccctccgga agcggtcgcg ggaaggaccg
    10381 tggcgtgacg ccgaggggcc ccagcctgtg gagcgggacg tcgccgacgc ggtcgtggcc
    10441 tccgcggacg tcgaacgagc ccttgcgctc gttcccgagg agttccgcgc gcccctcgtc
    10501 ctgcacgacc tccaggacct cccctacgag gagatcgccg acatcctcgg ggcgccgatc
    10561 ggcaccgtga agtccaggat ccaccgaggc cgcgtcgcgc tggcccgggc cctccggggg
    10621 gaacctccac ccccccggga tccgtcgaac gtgcaggaga cgacgtgagc catccagacg
    10681 agctgctggc cgagtacgtg gacggggccc tcgacgtttc ggcgcgagcc gaggtcgagg
    10741 cgcacttggc ctcgtgtggg gtctgtcggg ccgaggtggc cgaggccacc tcg
//
LOCUS       DASUTZ010000255         2922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1002596, whole genome shotgun sequence.
ACCESSION   DASUTZ010000255 DASUTZ010000000
VERSION     DASUTZ010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002596"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..1861
                     /locus_tag="VF097_10880"
     CDS             242..1861
                     /locus_tag="VF097_10880"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX6263338.1"
                     /translation="MQPEVDRRREALIRAGLTLASELSLPVVLQKLTELACLVADARY
                     GALGVLDRSGRRIERFVTHGVTSEERRAIGDLPRGRGILGVLIREARPLRLDRIRDDP
                     RSVGFPPHHPVMTSFLGVPIMVRGNVFGNLYLTEKRGGDPFTEEDEEAVMTLATQAGV
                     AVENARLYQEARAARERLEAIGRVTAAILEGREIDEVLRLIADRARDLAGGDLASVSV
                     PVGGGRLGLRVVVGELADQLEGEEYEAEGSIAEEVMRTGRPLLIPDLSEDPRKGQPVV
                     RIGRIGPGIYLPLSVGDRRFGTLLVANLLGGRSFDRDDADVVRMFAAQAAVALEYARF
                     REELQRLAVLEDRERIAQELHDGVIQSLFAVGMSLQATAGMAEDPETSERLNHAVDAV
                     DGAIRDLRNYIFGLRPGALADRQLAISLEELTKSFDESTGLTVIASIDRDVAARLVGR
                     ETEVLQAAREAISNAVRHSGGETVRVSLRQEGQDAILEVADDGSGFDPAHVEGRGQGL
                     GNLLHRADSLGGRLQIISDEGGTTVRITLPL"
     gene            1876..2541
                     /locus_tag="VF097_10885"
     CDS             1876..2541
                     /locus_tag="VF097_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006347979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6263339.1"
                     /translation="MASQPIRVMIVDDHEVVRQGVRSLLEAADDVVVAGEASGVSESV
                     ELAENTRPDVVIMDVRLVDGSGIEACREIRARRPETKVVMLTSFADDEALFASIMAGA
                     SGYVLKQVRGGDLLSSIRQVAEGHSLLDPAVTGPVLERLRKGKHLMKDEKLARLSPQE
                     ERILTQVADGKTNREIGEDLGLAEKTVKNYVSSILSKLEVARRAEAAAYLARHTGTPG
                     DQP"
     gene            complement(2543..>2922)
                     /locus_tag="VF097_10890"
     CDS             complement(2543..>2922)
                     /locus_tag="VF097_10890"
                     /inference="COORDINATES: protein motif:HMM:NF021536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HEX6263340.1"
                     /translation="AAAAADLDEEGRTAAVSAVLTTLMERLPRAHREHLMSHLPEDVR
                     SLAHHPRAPGEAATSIRTQREFAEAVEELARLDGAEGITGPVRALVVSGAVLTALRAL
                     IPEEAHDVAAVLPGELRDLWEAA"
BASE COUNT          421 a         1042 c         1057 g          402 t
ORIGIN      
        1 cccgtgctcg agcacggcca gggacccgat ctcctggtac tgcatgcggc ccgcggcgtc
       61 gagcagcgac tcgtcccggc tccccgccac gacctccggg cggtacaacg ctcgaacctt
      121 catcacacac ctcctccatc ggctcgcatg gccaccgtag gcaggcccgg ccccagctca
      181 caggtccgaa ggacccctcc gcgtcgggtc ctccgccccg tcctgctacc ctcgaaccgg
      241 catgcagcct gaggtcgatc gtcgtcgcga ggcgctgatc agggccgggc tcaccctcgc
      301 gtcagagctc tccctccccg tcgtcctcca gaagctgacc gagctcgcct gcctcgtggc
      361 cgacgcccgg tacggggcgc tcggggtgct cgatcgctcc ggccgccgga tcgagcgctt
      421 cgtcacgcac ggggtcacga gcgaggagcg gcgagcgatc ggggacctcc cgaggggccg
      481 cggcatcctc ggcgtcctca tcagggaggc ccgccccctc cggctcgatc ggatccggga
      541 cgacccgcgg tcggtgggct tccccccgca ccacccggtc atgacgtcct tcctcggcgt
      601 cccgatcatg gtgcggggga acgtgttcgg gaacctctac ctcaccgaga agcggggtgg
      661 ggacccgttc accgaggagg acgaggaggc ggtgatgacc ctggccacgc aggccggggt
      721 cgccgtcgag aacgccaggc tctaccagga ggcccgggcc gccagggagc gcctggaggc
      781 gatcggccgg gtcaccgcgg cgatcctcga ggggcgagag atcgacgaag tgctcaggct
      841 gatcgccgac cgcgcccgcg acctggccgg gggggatctg gcctcggtct ccgtccccgt
      901 gggtgggggg cgtctgggcc tgcgcgtggt ggttggcgag ctcgccgacc agctcgaagg
      961 cgaggagtac gaggccgagg gctccatcgc cgaggaggtg atgcgcaccg gccggcccct
     1021 cttgatcccc gacctctccg aggaccccag gaagggccag ccggtggtgc ggatcggccg
     1081 gatcggaccc gggatctacc tgcccctctc ggtcggggat cgtcgcttcg ggacgctgct
     1141 ggtggcgaac ctcctgggcg gacggtcgtt cgaccgggac gacgcggatg tcgtccggat
     1201 gttcgcggcc caggcagcgg tcgccctcga gtacgcccgg ttccgggagg agctccagcg
     1261 gctggccgtc ctggaggatc gggagcggat cgcccaggag ctccacgacg gggtcatcca
     1321 gtcgctgttc gctgtcggca tgtcgctcca ggccacggcc gggatggccg aggaccccga
     1381 gacgagcgag cgcctgaacc acgccgtgga cgcggtggac ggcgcgatcc gggaccttcg
     1441 caactacatc ttcgggctcc ggcccggcgc gctcgccgac cggcagctgg cgatctcgct
     1501 cgaggagctg acgaagagct tcgacgagag caccgggctc acggtgatcg cctcgatcga
     1561 tcgcgacgtg gcggcgcgcc tcgtgggcag ggagacggag gtcctccagg ccgctcggga
     1621 ggcgatctcc aacgccgtcc ggcactcggg aggagagacg gtgcgggtca gcctccggca
     1681 ggaggggcag gacgcgatcc tggaggtcgc ggacgatggg agtgggttcg acccggccca
     1741 cgtcgaaggg cgcggtcagg gcctggggaa cctgctccac cgcgctgact cgctcggagg
     1801 gcgtcttcag atcatctccg acgagggagg caccaccgta cgcatcaccc tccccctgtg
     1861 agaacattgg cggcgatggc cagccagccg atccgcgtga tgatcgtcga cgaccacgag
     1921 gtcgtccgtc agggcgtgcg gtcgctgctg gaggcggcgg acgacgtggt cgtcgcgggc
     1981 gaggcctccg gggtgtccga gtcggtggag ctcgccgaga acacccgccc cgacgtcgtg
     2041 atcatggatg tccgcctcgt ggacgggagc ggcatcgagg cgtgccgcga gatccgcgct
     2101 cggcggcccg agacgaaggt cgtgatgctg acctcgttcg ccgacgacga ggccctcttc
     2161 gcctcgatca tggccggagc ctccgggtac gtgctgaagc aggttcgcgg cggcgacctc
     2221 ctcagctcga tccggcaggt cgccgagggc cactcgctcc tcgatccggc cgtcaccggc
     2281 ccggtcctgg agcggctgcg caagggcaag cacctgatga aggacgagaa gctggcccgt
     2341 ctctcccccc aggaggagcg gatcctcacc caggtcgccg acggcaagac gaaccgcgag
     2401 atcggcgagg acctcgggct ggccgagaag accgtgaaga actacgtctc gagcatcctg
     2461 tccaagctcg aggtggcgag gcgcgccgag gcggctgcct acctggcccg ccacaccgga
     2521 acgccgggcg accagccctg acctaggcgg cctcccacag atcgcgaagc tcccccggaa
     2581 gcaccgcggc cacgtcgtgc gcttcctcgg ggatcagcgc tcgcagcgcc gtcaggacgg
     2641 ccccggacac cacgagcgcc cggaccggcc ccgtgatgcc ctcggccccg tcgaggcgtg
     2701 ccagctcctc caccgcctcc gcgaactccc gctgcgtgcg gatcgaggtc gccgcctccc
     2761 cgggagcccg agggtgatgc gcgagcgagc gcacgtcctc ggggaggtgg ctcatgaggt
     2821 gctcgcggtg agccctgggg aggcgctcca tcagggtcgt gaggacggcc gagaccgccg
     2881 ccgtccgccc ctcctcgtcg aggtcggcgg cggcggccgc gg
//
LOCUS       DASUTZ010000256        23668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_616809, whole genome shotgun sequence.
ACCESSION   DASUTZ010000256 DASUTZ010000000
VERSION     DASUTZ010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 23668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23668
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616809"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VF097_10895"
     CDS             complement(<1..213)
                     /locus_tag="VF097_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdenumtransferase MoeA"
                     /protein_id="HEX6263341.1"
                     /translation="MGHAHAHDDDRVLRGVEEVRSRILSSVEPLPPVELPLREAAGLV
                     LAEDVRAAFDLPPFASSAMDGFAVRAA"
     gene            complement(213..866)
                     /locus_tag="VF097_10900"
     CDS             complement(213..866)
                     /locus_tag="VF097_10900"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX6263342.1"
                     /translation="MRVRVLLFGALRERVGSGEAMLELPDGSTGGDVLRAVAERHPDA
                     APILDSVSVAVNLEVVPPEREVGSSDEVALLPPVAGGGGLLVGIREEPSVEEALAAAA
                     GDEAGATALFLGTVRDHSEAGAVEGLEYSAYEEMAERVMRGVAEEAREKWGLAGVAIL
                     HAVGDLGVGRPTIVAACSSAHREEAFEACRFLVEQVKSRLPVWKKERGPWGERWVNL"
     gene            complement(863..1363)
                     /locus_tag="VF097_10905"
     CDS             complement(863..1363)
                     /locus_tag="VF097_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEX6263343.1"
                     /translation="MGYRAAVLTVSDGVSRGVREDASGDAVEALLREAGYAVRRGVVP
                     DQRAAIEGALREMCAAADLVVTSGGTGLGPRDATPEATRAVIEREVPGLAEAMRAAGR
                     ASTPMADLSRGLVGSLRSSLIVNLPGSPAGAVEGLEAVLPALPHALDLLAGRTEHESS
                     GTRRGR"
     gene            1428..2576
                     /locus_tag="VF097_10910"
     CDS             1428..2576
                     /locus_tag="VF097_10910"
                     /inference="COORDINATES: protein motif:HMM:NF028293.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate/molybdate transporter"
                     /protein_id="HEX6263344.1"
                     /translation="MDRRLVGDAAGSLADLGVLVPLAAALILVNGLDAGAVLIGAGVL
                     VLAAGVAFRIPFPVQPLKALAAVAVASRVAPEVIHAAGLEIALVLLLLSVRGIADRVA
                     RVFTKPVVRALQVGVGMLLVVASARLVLDPPAVFRGTPPSPWPVLLAVAAFGGLWLAA
                     RAGRYEVALGILAAGALAAWLAAGPELAAPSLHLPSLSLPSAGAFGEALVLLVIPQLP
                     LTFGNAVVAVDDLAHEYFGPRAERVTPSRVCLSCGLGNVLAGLIGGMPMCHGAGGLTA
                     HVRLGARTARMNLLLGGALLALGLFFAPQAPAILGLLPVWALAALLALAGLRHALLVA
                     DLRGASLVVALIAGSIGALVGNLTVTLAVALVGAHAWRAVPKGQAPRS"
     gene            complement(2558..2749)
                     /locus_tag="VF097_10915"
     CDS             complement(2558..2749)
                     /locus_tag="VF097_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263345.1"
                     /translation="MADMGEAQRELAEIYERLKSLLAERGDELAPYEERNALKALAAL
                     WQVANGLDLDPGQLYDLGA"
     gene            complement(2742..4094)
                     /locus_tag="VF097_10920"
     CDS             complement(2742..4094)
                     /locus_tag="VF097_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX6263346.1"
                     /translation="MDTPYWNPRHETMPRGELEALQVRKLRNLVEWASAQVPFHAKRL
                     SDARVTADSIQSLEDLRRIPFFTRDEWMQGQLDQPPFGPILSAPEDAAIRYHMTSGTT
                     GRTPIRVLDSMKDWEWIAEMWCYGLWGFGVRPTDRMFVAFGYATFIGFWGLHYAGEKM
                     GCLTLPGGAMTTEQRVRTIEEMGATVVASTPTYALRMAQEARALGIDLASGPVQRLIL
                     SGEPAGSIPATKKLIEEQWGAKAGDTAGMTELGTIMIFECEAQPGGTHIIEDHYIEEV
                     IDPQTGEPVDYGEQGERVVTSFGRGFIPVLRYRTRDLVVKVPGGGCVCGRTFDIYDGG
                     IRGRVDDMKLVRGTNVYPRAVEAIVREIPEIDEFQIHLYTVEGIRDEIEVLVEIPDEG
                     ADVDGILARLGKELADAHESLRFGVKRAETGSLPRFELKAKRLVDEREVIGGEGEKRG
                     "
     gene            4188..4790
                     /locus_tag="VF097_10925"
     CDS             4188..4790
                     /locus_tag="VF097_10925"
                     /inference="COORDINATES: protein motif:HMM:NF015743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017128 - phospholipid scramblase activity
                     [Evidence IEA];
                     GO_process: GO:0017121 - plasma membrane phospholipid
                     scrambling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid scramblase-related protein"
                     /protein_id="HEX6263347.1"
                     /translation="MSLLERSRMFVHQKAKLVELTNEYRILDEEGQEIGVIRQEGQST
                     ARKVLRFVSSLDQFLTHTYAIYDADGTKLVELRRPAKFLKSRFLVSDGAGMPVGSIEQ
                     ENVVGKKRFRLLGQAGEHLGTFQAENLISWDFQILDAAGVPVGRVTKKWKGLGIEALT
                     TADNYMVEIDESVAGPFRMLAVASAASIDTALKQDAQGFN"
     gene            complement(4783..5469)
                     /locus_tag="VF097_10930"
     CDS             complement(4783..5469)
                     /locus_tag="VF097_10930"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEX6263348.1"
                     /translation="MGPRGGTGERRPLVPLDAYGRVLAAVLLAVGWTIASPEAAWSRA
                     VTVALFAGTVFVAFRASGVRGPVAGGVLVALGVAVVAAVLWEAAGEPAVTVVVGLALV
                     LASLGAIVRRLAAHPVVTTSTVAGALTVYLLVGMLFTFLFATIDLAVEEPILRGVGVA
                     RLGDFLYHSFITLTTVGYGDVAPGTDAMRAISVLEALIGQLYLVTIVAVTVARMGTRR
                     RGPEEDRSVS"
     gene            5539..6654
                     /locus_tag="VF097_10935"
     CDS             5539..6654
                     /locus_tag="VF097_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6263349.1"
                     /translation="MDAFALTDEQRRLIEEARGIAGDRLAGIEDEPGRVNRPLLRALA
                     EHGLLPHMFPEDGEVSAMTLCLLREAVAMESPEAETAMALQGLGAYPLLQSGQAEIVE
                     RWIPRVRSGEAVAAFALTEPAGGSDAAALQLRADSDGDGWRLTGEKAFISNAPDADVY
                     TLFARTTADAGARGVSAFVVPGDAEGLSGEHVELVAPHAIGHLRLDGVRVGPKDLLGE
                     LDRGFRVAMRTLDLFRPSVGAFAVGMAQAALDMAVAYAGERTSMGKPLREHQAVSQQL
                     GEMATRVEAARLLVYAAASAYDRGERVTKASAMAKLFATETAQAVVDASIQIHGARGL
                     VRGHPLERLYREVRSPRIYEGTSEIQREVVARELYRD"
     gene            complement(6651..7046)
                     /locus_tag="VF097_10940"
     CDS             complement(6651..7046)
                     /locus_tag="VF097_10940"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015794109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX6263350.1"
                     /translation="MTELFNPSELPRPSGFSHAAAASGRSVFVAGQIGEGSGEEVPEG
                     IAEQMDLACANVVKALRAAGAEPEQVVWMQIFTTDVAAYREASREIGEAYRKHFGKHF
                     PAMALVEVSGLFEPAALVELTAVAVVPDG"
     gene            complement(7043..7870)
                     /locus_tag="VF097_10945"
     CDS             complement(7043..7870)
                     /locus_tag="VF097_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HEX6263351.1"
                     /translation="MSPFRGSAGFTERWEHLDVGLEDGVCTVTFSRPDRLNALTFDAY
                     ADLRDLLSELPHSEDARVVLITGEGRGFCSGGDVHDIIGALQEMDPRGLLDFTRMTGT
                     VVQRMRECPLPIVAAINGVAAGAGAVIALASDLRIAARSASFRFLFTQVGLAGADMGS
                     AYLLPRLIGTARATELLLLGEGVDAERALEIGLVTQLVEDEELARAAGDLARRLASGP
                     ALAHETTKMLLTREQDMGLGPSIELEAFAQALLMKSEDHAEFYRAFLEGRDPRWSGR"
     gene            complement(7887..8276)
                     /locus_tag="VF097_10950"
     CDS             complement(7887..8276)
                     /locus_tag="VF097_10950"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX6263352.1"
                     /translation="MLVIDSSVAVHTASSATLGPLDGERLVAPPLLWSEVASALRGAV
                     WRGSISASLGAKSLARFLDMPIERRAPRRLYEEAWRTAEKLGWAKTYDAEYVALARIL
                     SCRLVTVDARLRRGGSRVAEIIGPEEL"
     gene            complement(8277..8522)
                     /locus_tag="VF097_10955"
     CDS             complement(8277..8522)
                     /locus_tag="VF097_10955"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6263353.1"
                     /translation="MLTVPEAAARTGRSAETIRRWIRAGKLRARKVGTQHVIEEEDLD
                     ELIDAETLPLPAEWRRTATGEPMPDWVTLIRRDRAGH"
     gene            complement(8561..9736)
                     /locus_tag="VF097_10960"
     CDS             complement(8561..9736)
                     /locus_tag="VF097_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020139400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6263354.1"
                     /translation="MAKADLNPSDLLDLEHLLSDEERAVQETVREWVGDRVLPEIADW
                     FEAGEIPSRDLAKELGAMGLLGMHLEGYGTAGASAVMYGLACLELEAGDSGIRSLVSV
                     QGSLAMFPIWRFGSEEQKQEWLPKMAAGEAIGCFGLTEPDFGSNPAGMRTSAKKDGSD
                     WVLNGTKMWITSGGVADVAIVWARTDDGVRGFIVPTDTAGFTTNDIHKKISLRASVTS
                     ELILENVRLPEDAVLPEVTGMKGPLSCLNEARYGIVWGSMGAARACFESALEYSKTRV
                     QFDRPIGGFQLTQRKLVDMAVELHKGLLLALHLGRMKDEKRARPEHISFGKLNNVREA
                     IEICREARGILGASGVTLEYPVIRHMNNLESVLTYEGTNEVHTLILGKVLTGLDAFQ"
     gene            complement(9773..11146)
                     /locus_tag="VF097_10965"
     CDS             complement(9773..11146)
                     /locus_tag="VF097_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEX6263355.1"
                     /translation="MADEPTQFLLQSDDLPTAWYNIMADVPGEPLPPLHPGTKEPVGP
                     DAFTAIFPMSLIEQEVSAERWIDIPGPVLDVYRLWRPTPMYRARRLEQALDTPARIYF
                     KYEGTSPVGSHKPNTAVAQAYYNREDGTKLLTTETGAGQWGSALAMAARFFGLECLVF
                     MVKASYEQKPYRRVLMETFGAEVRPSPTSTTEAGKKVLAEHPDSTGSLGIAISEAIEA
                     AVQDETTKYSIGSALNHVLLHQTVIGLEAKQQIEMAGERPDVIIGCVGGGSNYAGFSY
                     PFMADKLAGMLDARFLATEPAACPTLTRGEYRYDFADTGEMTPLVPMYTLGHTFVPAP
                     VHAGGLRYHGDAPSLSALVRAGLMEAKAYTQNPVFEAATQFTHAQGILPGPEPAHAIR
                     AVIDEALEAREAGEERVILFNLSGHGFFDLQAYDDYLNGRLPEHELDEEDLARGMAEV
                     PDVPVPG"
     gene            complement(11211..12131)
                     /gene="gnd"
                     /locus_tag="VF097_10970"
                     /pseudo
     CDS             complement(11211..12131)
                     /gene="gnd"
                     /locus_tag="VF097_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725049.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
     gene            complement(12089..13525)
                     /locus_tag="VF097_10975"
     CDS             complement(12089..13525)
                     /locus_tag="VF097_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX6263356.1"
                     /translation="MDELVRRIWDRDHTLWKPQPTEIVDRLGWLTIADAMRPRIRELE
                     RFADRVSVDGFDVALLLGMGGSSLAPEVFSRTFGNGGTRVEVLDTTHPAAVAAAEAHL
                     DLERTLFLVASKSGTTIETRSHLDRFWHLTGGDGRRFVAITDPGTPLEELARERGFRS
                     VFLAPPDVGGRYSAMSVFGLVPAALVRAPLERLLDGAVEMAEACRREEGNPGFALGEA
                     IGGAARVGADKLTFVLPEVIASFGDWIEQLVAESTGKKGTGIVPVVGEDVGPPEVYGD
                     DRFFVAYGEDPPDLGIRVDDGARESLGAEMFRWEFATAVAGHLLRVNPFDQPDVAAAK
                     KATSAALEEGYEDPGFDDLGPLLERVRPGGYLAIQAFLQPSEETREALQRARIALRDR
                     LKVATTLGFGPRFLHSTGQLHKGGPDTVVCVQVVDEERDVDVEIPGRPYTFGKLIDAQ
                     ALGDLRALRAAGRRAARVTLDTLTKEAS"
     gene            complement(13556..15001)
                     /gene="gatB"
                     /locus_tag="VF097_10980"
     CDS             complement(13556..15001)
                     /gene="gatB"
                     /locus_tag="VF097_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX6263357.1"
                     /translation="MSYETVIGLECHVELSTRTKMFCSCRNEFGAEPNTLVCPICLGH
                     PGTLPVPNERAIEHTITIGLALGSEIAPTSLFHRKNYFYPDMPKNYQISQYDLPLCVG
                     GALEVEVDGETRRVGITRVHLEEDTGKTTHAGATGRIAGADYALEDFNRAGVPLVEVV
                     SEPDLRSPEEARAYLVALRGLLRSLRVSDVKMEEGSLRCDANISVRPEGHEELGTKVE
                     IKNMNSIRSVERALAFEAERQRTALEAGETLVQETRHWDEDEGATSSMRSKEEAFDYR
                     YFPDPDLSPLEPEAERVDRLRSELPELPAARRARLESEHGLPAAQAAQLVDHDWDGFL
                     GEAVGEGADARATANWMTGDLAALLNEAGVELSQSKVTPKHLADLVRLVDDETISSAG
                     AKQVLAEMFASGADAEVVVEERGLAQISDESALGAIVDEVIADNPGPAEQFRGGKEGV
                     IGFLVGQVMQRSKGAANPQEAQRLLRERLRG"
     gene            complement(14998..16473)
                     /gene="gatA"
                     /locus_tag="VF097_10985"
     CDS             complement(14998..16473)
                     /gene="gatA"
                     /locus_tag="VF097_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX6263358.1"
                     /translation="MTELCDLAGHELASKLRAGEASALDVVESSLTRIEATEPSLHAF
                     LARTAEAAREQARAVDERLSAGHDLPTVAGIPLALKDVLTTKGVTTTCGSRILETYVP
                     PYDSTVWARLKEAGSVLVGKTNCDEFAMGSSNENSAFGPVHNPWDLERVPGGSSGGSA
                     AAVAAGQAVWALGTDTGGSVRQPAALSGVVGMKPTYGRPSRYGLIAFASSLDTVSTFT
                     RDVRDAATLLSAFVGHDPLDATSIQRPPEDYTEALEAGVDGLRIGVLRDWLGEGVEPG
                     VRARVEEAIGRLEGLGAKIGEATLPHADYALSAYYLIAPAECSSNLARYDGVRYGHRA
                     EGANVVEMMMKTRGEGFGDEVKRRIMLGTYALSAGYYEAYYGQAQKVRTLVLRDFEVA
                     WQEFDLLLSPTSPSVAFPIGAKADDPLSMYLSDVYTIPANLAGVPGISVPCGLDDQGL
                     PVGLQLMGKALDEATVLRAAYAFEQDLGFDARPPLLEEVAS"
     gene            complement(16470..16763)
                     /gene="gatC"
                     /locus_tag="VF097_10990"
     CDS             complement(16470..16763)
                     /gene="gatC"
                     /locus_tag="VF097_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018351362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX6263359.1"
                     /translation="MPVEIDIEHVARLARVGLTPEELERFREQLGVILEHAERVTEVA
                     AEAVPPTAHPIPMANVFRPDEPRDGLGRDEALANAPDREGDFFKVPPIVEEPE"
     gene            complement(16872..18908)
                     /gene="ligA"
                     /locus_tag="VF097_10995"
     CDS             complement(16872..18908)
                     /gene="ligA"
                     /locus_tag="VF097_10995"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX6263360.1"
                     /translation="MSEDRREPEERRRSDVRLRIEELREQIRHHQYRYYVEDDPEVSD
                     AEYDELVRELEKLETEFPEFVTPDSPTQRVGGQVSELFAPVEHRTPMLSLDNAFTWDE
                     LSEWGRRVERAVGRAGDFVCELKIDGVAVALTYEDGRYIRGATRGDGYTGEDITANIR
                     TVGGAPMRLRGEHSGVLEVRGEVYLPVKAFEEMNRELQEAGGRAFANPRNAAAGSLRQ
                     KDPGVTARRPLRLWVHGLGWADGRRFSRHSEALDHLREAGLPVNPDNAVLDSLEEVFA
                     FCEKWQEGRHDVDYEIDGVVVKVDQISLQEELGATAKAPRWAIAYKFPPEERTTLLRG
                     IFTHTGRTGKVTPFAQLEPVFVGGVTVTTATLHNESEVARKDVRPGDHVIVRRAGDVI
                     PEVVGPVMQKRKRGARRWKFPKECPSCGTPLVREESEADWRCPNREGCPSQNIEWLFH
                     FAGRGAMDIEHLGYKTGMALMDRGWVADPADVYFITEEQLAELPGFKEKSIGNLLGAI
                     EGSKDRPIWRLLVGLNIRHVGTTVARMLTRAFPSTERLAAAKMEEIEAVEGIGPEIAR
                     SVHEWFRRPESKRLLKKLRKAGVRMEDPEPGGPTPDGPLAGKRVVLTGGLESMSRDEA
                     TKAAEAAGARVTSSVSKSTDFVVAGTDPGSKYDKAVSLGVEIIDEEEFRRRLGL"
     gene            complement(18924..19670)
                     /locus_tag="VF097_11000"
     CDS             complement(18924..19670)
                     /locus_tag="VF097_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX6263361.1"
                     /translation="MTRALTLEGVHKSFAKVDVLRGVDLSVDQHEVIALIGASGSGKS
                     TLLRCVNLLEPIDAGRILLSGDEITLPSVNENEVRKRIGIVFQAFNLFPHMTVLRNVT
                     LGPIEALGLSREEAEARAEELLARFGLLDKRDEYPDRLSGGQQQRAAIVRAMAMQPDL
                     LLLDEVTSALDPELIAEVLDVIRELAEAGMTMILATHEMGFAKDIAGRVSFLDEGVIL
                     EEGPPDRMFAEPEHPRTRRFLQRIIAAGRL"
     gene            complement(19667..20545)
                     /locus_tag="VF097_11005"
     CDS             complement(19667..20545)
                     /locus_tag="VF097_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014672024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX6263362.1"
                     /translation="MSVTTPPGSPAPPMPPGDRRSRILGGGHWEGRYSVPIAVVSTLI
                     FFTVLVLLVINSPGWPEVRRSFFNAEIFLESFPDISRRFLKNVAAFLIAEVFILVLAM
                     FIAVLRSLPGPVFLPIRLLAVGYTDLFRGIPTILVMYILGFGAPALQLRIPKDPFFWG
                     IVALVLVYSAYVAEVYRAGIESVHPSQVAAARSLGLTRWQGLRFVVLPQAVRRVVPPL
                     LNDFVGLQKDTALLALIGVLEAFRQSQVDSAGNFNYTPYLATALLFVLITIPLARFTD
                     WMIARERRRHQAGSAL"
     gene            complement(20562..21455)
                     /locus_tag="VF097_11010"
     CDS             complement(20562..21455)
                     /locus_tag="VF097_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6263363.1"
                     /translation="MRKIAAVLMISGLVLAACGPEEEDETPEAAPTSSPTVDECATEN
                     LTLVNPGQLTIGTDNPAFPPWFEGGTPEGSSWEINDPSTGEGFESAVAYAVAEELGFG
                     EDEVEWVEVPFNQAFAPGPKDFDFDINQVSYRPRRDEAVDFSDSYYDVNQALVAVADT
                     PIAEASTVEELKQYTLGAPVGTTSFEYIDQNIQPDSDPRVYDTLNDSISGLENGQVDG
                     IVVDLPTAFFIVAVQLDNGTIVGQFPTVGQQEYFGMVFEEGNPLRDCVNQALDALRED
                     GTLDSIQQEWLADKTDAPVLE"
     gene            complement(21589..23049)
                     /locus_tag="VF097_11015"
     CDS             complement(21589..23049)
                     /locus_tag="VF097_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263364.1"
                     /translation="MLSLTGDAPPGSSVVAIISSGDTIGDFTFEGIPDGIGLMPGAPG
                     EVEVFVSHEQSTVPFLGFADFEDASVSQLTLDTSDAGVLEASVPIGPENGYIRFCSSF
                     MAGPAEGFKTYTFFANEESSDIIDVPSGAPYGPDPAVAPQRQAGYAVVLNAETGAFTQ
                     VAGMGRHNHENSVVIPGGWSQRGKGKKKGRAGKGAGPQIAILSTDDTFSPPTSQLYMY
                     LANRERHIWRDKGSLWGFQATAKNGSRVNPGNPFNGANDYLDLDPGDEIKGRFIRVPK
                     RIARGRTALPPQEALERWSNQHNVFQFIRLEDLAYDRHNPRVVYIADTGASRVVPDPD
                     TGRMVRGPSGTVGEADNGRIFKMVMNKSNPRKVNSLTVYADGDWEGTDVFVPMRAPDN
                     IDTSGKSLMVQEDADDAKVWAHDFASGSWTHVATVDDPDGESSGIVDASAFFGDGAWL
                     LDVQAHGSFESSEVIGDVTYKRENGQLLLMVLPGTT"
     gene            complement(23249..>23668)
                     /locus_tag="VF097_11020"
     CDS             complement(23249..>23668)
                     /locus_tag="VF097_11020"
                     /inference="COORDINATES: protein motif:HMM:NF042466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase beta-barrel
                     domain-containing protein"
                     /protein_id="HEX6263365.1"
                     /translation="EGEMVDAGGRVLGTHAGAHRFTIGQRRGLGVSTGERAYVLDVDA
                     GANRVVVGPGELLSRRGLIAERVSWVADAPDGPIEAEAQIRYNGEAAAAVVEPLPGGE
                     VRVTFRAPQRAIAPGQSVVFYAGDELLGGGAIARSLR"
BASE COUNT         3359 a         8745 c         8026 g         3538 t
ORIGIN      
        1 ggcggcgcgc accgcgaacc cgtccatcgc cgacgaggcg aacggcggca ggtcgaaggc
       61 cgcccggacg tcctccgcca ggacgagccc ggcggcctca cggaggggga gctccacggg
      121 tgggaggggc tccaccgacg agaggatccg cgaccgcacc tcctccacgc cccgcaggac
      181 ccgatcgtcg tcgtgggcgt gcgcgtgccc catcacaggt tcacccaccg ctcgccccag
      241 ggccctcgct ccttcttcca tacgggcagc cggctcttca cctgctccac gaggaagcgg
      301 caggcctcga aggcctcctc ccggtgcgcc gacgagcagg ccgccacgat cgtggggcgc
      361 cccacgccca ggtcgccgac ggcgtggagg atcgccacgc cggccagccc ccacttctcg
      421 cgggcctcct ccgccacgcc ccgcatcacg cgctccgcca tctcctcgta ggccgagtac
      481 tcgaggccct ccaccgctcc cgcctcggaa tggtcccgca ccgttcccag gaagagcgcg
      541 gtggctccgg cctcatcgcc tgccgccgcg gccagcgcct cctcgaccga gggctcctcc
      601 cggataccga ccaggagccc gcccccgccc gccacggggg gcaggagcgc cacctcgtcc
      661 gaagagccga cctcccgctc gggcggcacg acctccaggt tcacggccac ggacacgctg
      721 tccaggatcg gcgcggcatc ggggtgccgc tcggccacgg cgcggaggac gtcgccgccc
      781 gtggagccgt ccgggagctc gagcatggcc tccccggacc cgacccgctc ccgcagggcg
      841 ccgaacagca ggacccggac cctcatcggc cccgcctcgt gccgctggac tcgtgctccg
      901 tccggcccgc gaggagatcg agggcgtgcg ggagggccgg cagcacggcc tcgaggccct
      961 ccaccgcccc ggcggggctc cccgggaggt tcacgatcag cgaggacctc agggacccga
     1021 ccagcccgcg ggacaggtcc gccatcggcg tcgaggcccg gcccgccgcg cgcatcgcct
     1081 cggccaaacc cgggacctcc cgctcgatca cggcgcgggt ggcctccggc gtcgcgtcgc
     1141 gtggcccgag gccggtgccc ccgctcgtca ccacgaggtc cgcggcggca cacatctcgc
     1201 ggagggcgcc ttcgatcgcc gcccgctggt cgggcacgac gccgcggcgc accgcgtagc
     1261 ccgcctcgcg cagcagcgcc tcgacggcgt ctcccgaggc gtcctcccgg acgcctcggg
     1321 agacgccgtc gctcacggtc aggaccgcgg cccgatatcc catcgggctc atgataggca
     1381 gggagcccgc gacgcgcgcc gtccggcccc gtgccagact cgggcccatg gaccggcgcc
     1441 tcgtgggcga cgcggcgggg agtctcgccg acctcggcgt gctcgtcccg ctggccgcgg
     1501 ccctgatcct cgtgaacggc ctcgatgccg gggctgtgct gatcggggcc ggggtcctcg
     1561 tgctcgccgc cggcgtcgcc ttccggatcc ccttccccgt ccagcccctc aaagccctgg
     1621 ccgcggtggc ggtcgcctcg agggtggccc ccgaggtgat ccacgcggcg ggcctggaga
     1681 tcgccctcgt gctgctcctg ctctcggtcc gcgggatcgc cgaccgcgtg gcccgcgtgt
     1741 tcacgaaacc cgtggtccgt gcgctgcagg tcggggtcgg gatgctcctg gtcgtggcct
     1801 cggcgcgcct cgtcctcgac cccccggcgg tgttccgagg aaccccgccc tcaccctggc
     1861 ccgtcctgct ggccgtcgcg gccttcggag ggctgtggct ggcggcccgg gccggccgct
     1921 acgaggtggc cctggggatc ctggcggcgg gcgcgctggc tgcctggctc gcggccgggc
     1981 ccgagctcgc ggcgccctcg ctccacttgc cctcgctctc cctcccctcc gcaggcgcgt
     2041 tcggggaggc gctcgtcctc ctcgtgatcc cccagctccc gctcacgttc gggaacgcgg
     2101 tggtggccgt ggacgacctc gcccacgagt acttcgggcc gagagccgag agggtcaccc
     2161 cctcgcgcgt ctgcctctcc tgcggcctgg ggaacgtcct ggccggcctg atcggcggga
     2221 tgcccatgtg ccacggcgcc ggaggcctca ccgcgcacgt ccgcctcggc gcgcggaccg
     2281 cccggatgaa cctccttctc gggggagcgc tcctcgctct ggggctcttc ttcgcgccgc
     2341 aggccccggc catcctgggc ctgctccccg tgtgggcgct ggccgcgctc ctggcgctgg
     2401 ccggcctccg ccacgccctc ctggtggcgg acctgcgcgg cgcgagcctc gtcgtcgccc
     2461 tgatcgcggg atcgatcggc gcgctcgtgg ggaacctcac ggtgacgctg gcggtcgcgc
     2521 tcgtcggcgc gcacgcctgg cgagccgtgc cgaagggtca ggcgcccagg tcgtagagct
     2581 ggccggggtc gaggtcgagc ccgttcgcca cctgccacag cgcggccagc gccttcagcg
     2641 cgttccgctc ctcgtagggc gcgagctcgt ccccccgctc ggccagcagc gacttcagcc
     2701 gctcgtagat ctcggccagc tcgcgctggg cctcgcccat gtcagccacg cttctccccc
     2761 tcgccgccga tcacctctcg ctcgtccacg agcctcttgg ccttcagctc gaaccgaggc
     2821 agggaccccg tctccgcccg cttcacgccg aaccgcagcg actcgtgcgc gtcggccagc
     2881 tccttcccca gcctggccaa gatcccgtcc acatcggccc cctcatcggg gatctccacc
     2941 aggacctcga tctcgtcccg gatcccctcc acggtgtaga ggtggatctg gaactcgtcg
     3001 atctcgggga tctcgcggac gatcgcctcg accgcgcgcg ggtagacgtt cgtcccccgg
     3061 acgagcttca tgtcgtccac gcgcccccgg atgccgccgt cgtagatgtc gaaggtgcgg
     3121 ccgcagacgc atcccccgcc cggcaccttc accacgaggt cccgcgtccg gtaccggagc
     3181 accgggatga atccccggcc gaacgacgtc acgacccgct cgccctgctc accgtagtcc
     3241 acgggctcgc cggtctgcgg gtcgatgacc tcctcgatgt agtggtcctc gatgatgtgg
     3301 gtcccgcccg gctgggcctc gcactcgaag atcatgatcg tgcccagctc ggtcatcccc
     3361 gcggtgtcgc cggctttcgc accccactgc tcctcgatca gcttcttggt ggcggggatc
     3421 gagccggcgg gctcccccga gaggatcagc cgctggaccg gcccgctcgc caggtcgatc
     3481 cccagcgccc gggcctcctg ggccatccgc agcgcgtagg tcggggtgga ggcgaccacg
     3541 gtggccccca tctcctcgat cgtccggacc cgctgctcgg tggtcatcgc gcctcccggc
     3601 agggtcaggc agcccatctt ctcccccgcg tagtggaggc cccagaaccc gatgaacgtc
     3661 gcgtacccga acgccacgaa catgcggtcg gtgggccgca ccccgaagcc ccacagcccg
     3721 tagcaccaca tctccgcgat ccactcccag tccttcatcg agtcgaggac gcggatgggc
     3781 gtgcggccgg tggtcccgga ggtcatgtgg tagcggatcg cggcgtcctc cggcgccgag
     3841 aggatcggac cgaacggcgg ctggtcgagc tggccctgca tccactcgtc gcgggtgaag
     3901 aacgggatgc ggcgaaggtc ctcgaggctc tggatcgagt cggcggtcac ccgcgcgtcg
     3961 gagagccgct tggcgtggaa ggggacctgg gcgctcgccc actccacgag gttccggagc
     4021 ttccgcacct ggagcgcctc cagctcccct cggggcatgg tctcgtgacg ggggttccag
     4081 tacggcgtgt ccatctgcac cctccccggt cgatcggcga catcctaccg aggggcccgc
     4141 ccgccgcgcg accgtggggt acgcttcctc accgagggga ggtgtccatg tcgctgctcg
     4201 agcgatcgcg catgttcgtc caccagaagg cgaagctggt ggagctcacg aacgagtacc
     4261 ggatcctcga cgaggagggc caggagatcg gcgtgatccg gcaggagggc cagtcgacgg
     4321 ccaggaaagt actccgcttc gtctcctcgc tcgaccagtt cctgacccac acctacgcga
     4381 tctacgacgc cgacggcacc aagctcgtcg agctcaggcg cccagcgaag ttcctgaagt
     4441 cgagattcct ggtgagcgac ggcgcgggca tgcccgtggg ctcgatcgag caggagaacg
     4501 tcgtaggcaa gaagcggttc cggctcctcg ggcaggccgg cgagcacctc ggcacgttcc
     4561 aggccgagaa cctgatctcg tgggacttcc agatcctcga cgctgccggg gtccccgtgg
     4621 ggcgcgtgac caagaagtgg aagggcctgg ggatcgaggc gctgaccacg gccgacaact
     4681 acatggtcga gatcgacgag tcggtggcag ggccgttccg gatgcttgcg gtggcgtctg
     4741 cggccagcat cgacaccgct ctgaagcagg acgcgcaggg cttcaactga cggagcgatc
     4801 ctcctcgggc cctcggcgcc tggtccccat cctcgccacg gtgaccgcca cgatcgtgac
     4861 gaggtagagc tgtccgatca gggcctccag gacggagatg gcccgcatcg cgtcggttcc
     4921 aggcgccacg tccccgtacc ccacggtcgt gagcgtgatg aacgagtggt agaggaagtc
     4981 gccgaggcga gccaccccga cgcctcgaag gatcggctcc tccaccgcga ggtcgatcgt
     5041 ggcgaagagg aacgtgaaca gcatccccac gaggaggtag acggtcagcg cgcccgccac
     5101 ggtgctcgtg gtcacgacgg gatgcgcagc cagccggcgg acgatcgctc caagcgaggc
     5161 cagcacgagg gcgaggccga ccacgacggt cacggccggc tcaccggccg cctcccacag
     5221 aacagccgcg accaccgcca cgcccagggc cacgaggacc cctcccgcca ccggcccccg
     5281 aacgcccgaa gcgcggaacg cgacgaagac ggttcccgcg aacagcgcaa cggtgacggc
     5341 gcgggaccac gctgcctcgg gagaggcgat cgtccaccca accgccagca ggaccgccgc
     5401 caggacccgg ccgtaggcat ccaggggaac caggggccga cgctccccgg tccctcccct
     5461 cgggcccatg tcccgtcgat cgtacccggg ccgaggcccg gttccgtgga gcccgtgcca
     5521 gccggtagag tgcgcgagat ggacgcgttc gcgctgaccg acgagcagcg gcggctgatc
     5581 gaggaagcgc gcgggatcgc gggcgaccgc ctggcgggga tcgaggacga gcccgggcgc
     5641 gtgaaccggc cgctgctcag agcgctcgcc gagcacggac ttctccccca catgttcccg
     5701 gaggacggcg aggtctccgc gatgacgctc tgcctcctgc gcgaggccgt ggcgatggag
     5761 tcgcccgagg cggagacggc gatggcgctc cagggcctcg gggcctatcc cctcctccaa
     5821 tcggggcagg cggagatcgt ggagcgctgg atccctcggg tgcgctcggg cgaggcggtg
     5881 gcggccttcg cactgaccga gcccgcaggc gggagcgacg cggccgcgct gcagctccgc
     5941 gccgactccg atggggacgg ctggcggctc accggcgaga aggctttcat ctcgaacgcc
     6001 cccgacgccg acgtgtacac gctgttcgcg cggaccacgg cggacgcggg ggcccggggc
     6061 gtgagcgcgt tcgtcgtccc cggcgacgcg gaaggacttt cgggggagca cgtcgagctc
     6121 gtcgcgccgc acgcgatcgg gcacctgcgc ctcgacggcg tccgggtcgg ccccaaggat
     6181 ctcctggggg agctcgatcg gggcttccgc gtggcgatgc gcacgctgga cctcttccgc
     6241 cccagcgtcg gcgcgttcgc ggtggggatg gcccaggcgg cgctggacat ggcggtggcg
     6301 tacgcgggcg agcggacgtc catggggaag ccgctccggg agcaccaggc cgtgtcccaa
     6361 cagctgggcg agatggccac gcgggtcgag gccgcgcgtc tgctcgtcta cgcggcggcg
     6421 agcgcctacg accgaggaga gcgcgtcacg aaggccagcg cgatggcgaa gctgttcgcc
     6481 accgagacgg cacaggcggt cgtcgacgcc tcgatccaga tccacggcgc ccgcgggctc
     6541 gtgcgcgggc atccgctgga gcgcctgtac cgcgaggtgc gatccccgag gatctacgag
     6601 ggaacgagcg agatccagcg cgaggtcgtg gcccgcgagc tctaccggga ctagccgtcc
     6661 gggacgaccg ccacggccgt cagctccacg agcgcggccg gctcgaacag ccccgacacc
     6721 tcgacgagcg ccatcgcggg gaagtgcttg ccgaagtgct tccggtacgc ctccccgatc
     6781 tcccgcgacg cctcgcggta ggccgccacg tcggtggtga agatctgcat ccagaccacc
     6841 tgctcgggct cggcgccggc cgctcgaagg gccttcacca cgttggcgca cgccaggtcc
     6901 atctgttccg cgatcccctc ggggacctcc tcgcccgacc cctccccgat ctgcccggcc
     6961 acgaacaccg agcgtcccga cgcggccgcg gcgtgggaga agccgctcgg cctcgggagc
     7021 tccgatgggt tgaacagctc ggtcatcggc cgctccacct cgggtcccgg ccctccagga
     7081 acgcccggta gaactcggcg tgatcctcgc tcttcatcag cagggcctgg gcgaaggcct
     7141 cgagctcgat cgacgggccg aggcccatgt cctgctctcg cgtcagcagc atcttcgtgg
     7201 tctcgtgggc cagcgccggg cccgacgcca gccttcgcgc caggtcgccc gcggccctgg
     7261 ccagctcctc gtcctcgacc agctgtgtga ccagcccgat ctccagcgcg cgctcggcat
     7321 ccaccccctc gcccagcagc agcagctccg tggcgcgggc ggtcccgatc agcctgggga
     7381 gaaggtacgc ggagcccatg tccgcgcccg ccaacccgac ctgcgtgaag aggaacctga
     7441 acgaggcgga gcgcgcagcg atccgcaggt ccgacgcgag cgcgatcacc gcgcccgcgc
     7501 ccgccgccac cccgttgatc gccgccacga tcggcagggg gcactcccgc atccgctgga
     7561 ccacggtccc ggtcatccgg gtgaagtcga ggagcccgcg cgggtccatc tcctggaggg
     7621 ctccgatgat gtcgtgcacg tcgccgcccg agcagaagcc gcggccctcg ccggtgatca
     7681 ggaccacgcg ggcatcctcg ctgtggggca gctccgagag caggtcgcgc aggtccgcgt
     7741 atgcgtcgaa ggtcagcgcg ttgagccggt ccgggcgcga gaacgtgacc gtgcacacgc
     7801 cgtcctcgag accgacgtcg aggtgctccc agcgctcagt gaaccccgcc gacccccgga
     7861 acgggctcaa ccgctccccc tcgccttcac agctcctccg gtccgatgat ctcggcgacc
     7921 ctcgatccgc cccttcgcag cctcgcatcc acggtcacga ggcgacacga caggatcctc
     7981 gccagggcaa cgtattcggc atcgtaggtc ttcgcccacc cgagcttctc ggccgttcgc
     8041 cacgcctcct cgtacaacct gcggggcgct cgacgttcga tcggcatgtc gaggaagcga
     8101 gccaggctct tcgccccgag actcgctgag atgctccccc gccagaccgc gccacgcaac
     8161 gcggaggcca cctccgacca gaggaggggt ggggccacca gacgctcacc gtcgaggggc
     8221 ccgagcgtcg cagatgaggc agtatggacc gcgacgctcg agtcgatgac gagcactcag
     8281 tgccccgctc gatcccgtcg gatcagggtc acccaatccg gcatcggctc gccggtcgcg
     8341 gttcgacgcc actcagccgg caacgggagc gtctccgcgt cgatgagctc gtcgaggtcc
     8401 tcttcctcga tcacgtgctg tgtgcccacc ttgcgggcgc ggagcttccc cgcacggatc
     8461 catcgacgga tcgtctcggc ggatcggccc gtgcgggcgg ccgcctcagg aacggtcagc
     8521 ataggtgtag catacaacac tcggttccag agggggctcc ctactggaag gcgtcgaggc
     8581 cggtgagaac cttccccagg atcagcgtgt ggacctcgtt cgtgccctcg taggtgagga
     8641 ccgactccag gttgttcatg tggcggatca ccgggtactc cagcgtcacc ccgctggccc
     8701 cgaggatccc gcgggcctcg cggcagatct cgatcgcttc gcgcacgttg ttgagcttcc
     8761 cgaagctgat gtgctcgggc cgggcccgct tctcgtcctt catccgcccg aggtggagcg
     8821 ccagcagaag ccccttgtgc agctccaccg ccatgtccac gagcttcctc tgggtcagct
     8881 ggaagccgcc gatcggcctg tcgaactgca cccgggtctt cgagtactcc agcgcactct
     8941 cgaagcaggc gcgcgccgcc cccatcgagc cccagacgat cccgtagcgg gcctcgttga
     9001 ggcaggacag cggccccttc atgccggtga cctcggggag gaccgcgtcc tcgggcaggc
     9061 gcacgttctc caggatcagc tcgctcgtca ccgaggcccg cagcgagatc ttcttgtgga
     9121 tgtcgttcgt ggtgaagccc gccgtgtcgg tggggacgat gaacccgcgc accccgtcgt
     9181 cggtccgggc ccagacgatc gccacgtcgg ccaccccgcc gctcgtgatc cacatcttcg
     9241 tgccgttgag gacccagtcg gagccgtcct tcttggcgga ggtccgcatc ccggccgggt
     9301 tcgacccgaa gtcgggctcg gtgagcccga agcagccgat cgcctcgccc gccgccatct
     9361 tcggcagcca ctcctgcttc tgctcctcgg acccgaacct ccagatcggg aacatcgcca
     9421 acgagccctg gaccgacacg agcgagcgga tgccgctgtc gccggcctcg agctccaggc
     9481 acgccaggcc gtacatcacg gcggaggcgc cggccgtgcc gtagccctcg aggtgcatcc
     9541 ccaggaggcc catcgcaccg agctctttgg ccagatcgcg cgaggggatc tcgcccgcct
     9601 cgaaccagtc ggcgatctcg gggaggacgc ggtcgcccac ccactcgcgt acggtctcct
     9661 gcaccgcgcg ctcctcgtcg gagagcaggt gctccaggtc gagcaggtct gacgggttca
     9721 ggtcggcctt ggccatcggc cctccagggt atcggatgcg gtcgcccggc tcctacccag
     9781 ggaccgggac gtccggaacc tcggccattc cccgcgccag gtcctcctcg tccagctcgt
     9841 gctccggcag gcggccgttc aggtagtcgt cgtaggcctg gaggtcgaag aacccgtgcc
     9901 cggagaggtt gaagaggatc acgcgctcct cccccgcctc gcgcgcctcg agcgcctcgt
     9961 cgatcaccgc ccggatcgcg tgggccggct cgggccccgg gaggatcccc tgggcgtggg
    10021 tgaactgcgt ggccgcctcg aacaccgggt tctgcgtgta ggccttcgcc tccatgaggc
    10081 ccgcccgcac gagcgcggag agcgagggcg cgtcgccgtg gtaccggagg ccgcccgcgt
    10141 gcacgggcgc cgggacgaac gtgtggccca gcgtgtacat cgggacgagc ggcgtcatct
    10201 cccccgtgtc ggcgaagtcg taccggtact cacctcgggt gagggtgggg cacgcggctg
    10261 gctccgtggc caggaagcga gcgtccagca tcccggccag cttgtcggcc atgaacgggt
    10321 agctgaaccc cgcgtagttc gagcccccac ccacgcagcc gatgatcaca tcgggccgct
    10381 cccccgccat ctcgatctgc tgcttggcct ccagaccgat caccgtctgg tgcagcagca
    10441 catggttgag cgccgagccg atcgagtact tcgtcgtctc gtcctggacg gccgcctcga
    10501 tcgcctcgct gatggcgatg cccaacgagc cggtggagtc ggggtgctcg gccagcacct
    10561 tcttccccgc ctcggtagtg gaggtgggcg aggggcggac ctcggccccg aaggtctcca
    10621 tcagcacccg gcggtagggc ttctgctcgt aggaggcctt caccatgaag accaggcact
    10681 cgaggccgaa gaacctcgcg gccatggcca gggccgagcc ccactgcccc gcgccggtct
    10741 cggtcgtgag gagcttcgtg ccgtcctccc tgttgtagta ggcctgagcc accgcggtgt
    10801 tgggcttgtg ggagccgacg ggcgaggtgc cctcgtactt gaagtagatg cgggcgggag
    10861 tgtcgagggc ctgctccagg cggcgggccc gatacatggg tgtgggcctc cacagccggt
    10921 agacgtcgag gacgggcccg gggatgtcga tccaccgctc ggcggagacc tcctgctcga
    10981 tgagcgacat ggggaagatc gcggtgaagg cgtcggggcc gacgggctcc ttcgtccccg
    11041 ggtggagcgg cgggagcggc tcgccgggga cgtcggccat gatgttgtac caggcggtcg
    11101 gcaggtcgtc ggactgaagc aggaactggg tcggctcgtc ggccatcggt cctcctcgtg
    11161 ccggggccac gattctgtgc gcggtccccc cgcgcggtca actcgctcgc tcagtcgggg
    11221 ggctggacgg cgtggccgcc gaactcgcgg cggagcgcgg ccaccacccg cattgcgaag
    11281 gagttgtcgt cgcggctcgc gaagcgggag aacagggcgg ccgcgatcgc cggggccggg
    11341 acggcctcct cgatcgcctg ggccaccgtt ccccgcccga atccggagtc gtccacccag
    11401 gcggccagct ccaagagctc ggggtcgcgc tcgagagcgg ccacgatcag gtcgagcagc
    11461 cacgagcgga tcaccgtgcc gtggcgccag gcctcggcca ccgcgtgcag gtcgatgtcg
    11521 gggaaatcct cggagcgatg gaggatctcg agcccctcgg cgtaggcctg catcaccccg
    11581 tactcgatcc cgttgtggat catcttcgtg aagtgacccg atcccgaggg gcccacgtgg
    11641 acccagtggc cgtcggtcgc gagcgcgtcg aggatcggcg cgacgcgacc gacggcctcg
    11701 tcgggaccgc cgaccatcag cccgtagccg ccctcgaggc cccagactcc gccggagacg
    11761 cccgcgtcga cgtaggcgat gccgcggtcg gcgagctcgc cggcccgacg catcgagtcc
    11821 cggaagtgcg agttggcgcc gtccacgatg aggtctccct cggagaggag gccggcgagc
    11881 tcgccgacca ccgcctcggt ggcggcgccg gcggggacca tcgaccaggc gatccgtgga
    11941 ggctccagcc gttcgacgag ctcctcgtac gaggccacgt cgcggtgacc ctccttccgc
    12001 tcgtacccaa ggacctcgtg ccccgtgccc cgcaggcggt ccctcatgtt cccgcccatc
    12061 cggccgaggc ccaccatccc aatccgcatc agctcgcctc cttggtcagg gtatcgaggg
    12121 tgacgcgcgc tgcgcgccgc ccagcggcgc gcagcgcgcg cagatctccc agagcctgcg
    12181 cgtcgatgag cttcccgaac gtgtacggcc ttcccgggat ctccacgtcc acgtcccgct
    12241 cctcatccac gacctggacg cacacgaccg tgtccggccc acccttgtgc agctggccgg
    12301 tcgagtgcag gaaccggggc ccgaacccga gcgtggtggc caccttcagc cggtctcgca
    12361 gggcgatcct ggcccgctgc agcgcctccc tcgtctcctc gctcggctgc aggaacgcct
    12421 ggatcgccag gtagccgccc ggtcgcaccc tctccagcag cggcccgagg tcatcgaacc
    12481 ccggatcctc gtacccctcc tccagcgcgg ccgaggtggc cttcttcgcc gccgccacgt
    12541 ccggctggtc gaacgggttc acgcggagca gatgccccgc cacggcggtg gcgaactccc
    12601 accggaacat ctccgcgcca agcgactccc gcgcgccgtc gtcgacccgg atccccaggt
    12661 ccgggggatc ctcaccgtag gccacgaaga agcggtcgtc gccgtacacc tcgggcgggc
    12721 caacgtcctc gcctaccacg gggacgatcc ccgtgccctt cttccccgtg gattcagcga
    12781 cgagctgctc gatccagtca ccgaacgagg caatcacctc cggcagcacg aacgtcagct
    12841 tgtcggcccc cacgcgggcc gcgcccccga tcgcctcgcc gagcgcgaag cccgggttgc
    12901 cctcctcgcg ccggcaggcc tccgccatct cgacggcgcc gtccagcagc cgctccagcg
    12961 gcgcgcggac gagcgccgcg gggacgagcc cgaacaccga catcgcggaa tagcgaccgc
    13021 cgacatcggg gggcgccagg aagacggagc ggaacccgcg ctcgcgcgcc agctcttcca
    13081 gcggcgtgcc cgggtcggtg atcgccacga accggcgccc gtcgcccccc gtgaggtgcc
    13141 agaaccggtc gaggtgcgag cgagtctcga ttgtggtccc cgacttcgac gccacgagga
    13201 agagcgtccg ctcgagatcg agatgggcct cggccgcggc caccgccgcg gggtgcgtcg
    13261 tgtccaggac ctcgacgcgg gttccaccgt ttccgaatgt tctcgagaac acctccgggg
    13321 ccaggctgga accgcccatc cccaggagca gggcgacgtc gaagccgtct acggagacac
    13381 gatcggcgaa ccgttccagc tccctgatcc gcggccgcat cgcatcggcg atcgtgagcc
    13441 atccgagacg gtcgacgatc tccgtcggtt gcggcttcca gagggtgtgg tcccgatccc
    13501 agatccgccg cacgagctcg tccatcgagc cgagtctagt gcgcgggccc tgcgactatc
    13561 cgcgaagccg ttcgcggagg aggcgctggg cctcctgggg gttggcggcg cccttggagc
    13621 gctgcatcac ctgcccgacg aggaagccga tcacgccctc ctttccgccg cggaactgct
    13681 cggcggggcc agggttgtcg gcgatcacct cgtccacgat cgccccgagc gcggactcgt
    13741 cggagatctg ggccaaaccc cgctcctcga cgaccacctc agcgtccgcc ccggaggcga
    13801 acatctcggc cagcacctgc ttggcgccgg cgctcgagat cgtttcgtcg tccacgaggc
    13861 ggacgaggtc cgcgagatgc ttcggggtca ccttcgactg cgacagctcg acgcccgcct
    13921 cgttcagcag agcggccagg tcccccgtca tccagttggc agtcgcccgc gcatcggcac
    13981 cctcgcccac cgcctccccc aggaacccgt cccagtcgtg gtccacgagc tgggcggcct
    14041 gggccgcagg gaggccgtgc tcggactcca gccgggcccg ccgagccgcg gggagctccg
    14101 gcagctctga tcgcagccgg tccacccgct ctgcctcggg ctccagcggc gagaggtccg
    14161 ggtcggggaa gtagcggtag tcgaacgcct cctccttcga tcgcatggag gaggtcgcgc
    14221 cctcatcctc gtcccagtga cgggtctcct ggaccagcgt ctcccccgcc tcgagcgcag
    14281 tccgctggcg ctcggcttcg aaggccagcg cccgctccac gctgcggatc gagttcatgt
    14341 tcttgatctc taccttcgtc ccgagctctt cgtgaccctc cggcctcacc gagatgttcg
    14401 cgtcgcagcg gagcgagccc tcctccatct tcacgtccga gacgcggagc gagcgcagca
    14461 gcccgcgcag cgccacgagg taggcgcggg cctcctcggg cgagcggaga tcgggctccg
    14521 agacgacctc cacgagcggc acgcccgccc ggttgaagtc ctcgagcgcg tagtcggcgc
    14581 cggcgatccg gcccgtggcg ccggcgtgcg tcgtcttccc cgtgtcctcc tcgaggtgca
    14641 cgcgcgtgat cccgacgcgt cgcgtctcgc cgtcgacctc gacctccagc gcgccgccca
    14701 cgcacagcgg gaggtcgtac tggctgatct ggtagttctt cggcatgtcc ggatagaagt
    14761 agttcttccg atggaagagc gaggtcggcg cgatctccga gcccagcgcc agaccgatcg
    14821 tgatcgtgtg ctcgatcgcc cgttcgttgg ggacggggag cgtgccggga tggcccaggc
    14881 agatcgggca gacgagggtg ttgggctcgg ccccgaactc gttccgacac gagcagaaca
    14941 tcttcgtgcg ggtcgagagc tcgacgtggc actccaggcc gatcacggtc tcgtagctca
    15001 cgaggccacc tcctcgagca gcggaggccg ggcgtcgaag ccgaggtcct gctcgaacgc
    15061 gtacgccgca cggagcacgg tggcctcgtc cagtgccttg cccatgagct ggagacccac
    15121 gggcagcccc tggtcgtcga gcccgcaggg gacgctgatg cctgggaccc ccgccaggtt
    15181 cgcggggatc gtgtagacgt cggagaggta catcgagagc gggtcgtcgg cctttgcccc
    15241 gatcgggaag gcgaccgacg gcgaggtcgg cgacagcagg aggtcgaact cctgccaggc
    15301 cacctcgaag tcgcgcagga ccagtgtcct gaccttctgg gcctggccgt agtaggcctc
    15361 gtagtagccg gccgagagcg cgtaggtgcc gagcatgatg cggcgcttga cctcgtcccc
    15421 gaagccctcg ccgcgcgtct tcatcatcat ctcgaccacg ttggcgccct cggcccggtg
    15481 gccgtagcgg acgccgtcgt agcgggccag gttcgaggag cactcggccg gagcgatcag
    15541 gtagtaggcg ctgagcgcgt agtcggcgtg aggcagcgtg gcctcgccga tcttcgcgcc
    15601 gaggccctcg aggcggccga tggcctcctc gacgcgggcg cggacgccgg gctccacgcc
    15661 ctcccccagc cagtccctga ggacgccgat ccggaggccg tcgacgccgg cttcgagcgc
    15721 ctccgtgtag tcctcggggg gccgctggat gctcgtggcg tcgagcgggt cgtgccccac
    15781 gaaggccgac agcagcgtgg ccgcgtcccg gacgtcccgc gtgaaggtcg agacggtgtc
    15841 gagcgacgag gcgaacgcga tgaggccgta gcgcgaggga cgcccgtagg tgggcttcat
    15901 cccgacgacc ccggagagcg cggcgggctg gcgcacggag ccgccggtgt cggtgccgag
    15961 ggcccagacg gcttgtcctg cggccaccgc ggccgccgac ccgcccgacg accctccggg
    16021 cacgcgctcc aggtcccagg ggttgtggac cggcccgaac gcggagttct cgttcgagga
    16081 tcccatcgcg aactcatcgc agttggtctt gccgacgaga accgatcccg cctccttgag
    16141 ccgtgcccag accgtcgagt cgtacggggg gacgtaggtc tcgaggatcc gcgagccgca
    16201 ggtcgtggtg acgcctttcg tggtgaggac gtccttcagg gcgagcggga tccctgcgac
    16261 tgtgggtagg tcgtggccgg ccgacagccg ctcgtcgacg gcgcgggcct gctcacgggc
    16321 cgcctcggcg gtccgggcca ggaacgcgtg caggctcggc tcggtggcct cgatccgcgt
    16381 cagcgatgac tcgacgacgt cgagcgcgga ggcctcgccg gcccggagct tggaggcgag
    16441 ctcgtggccg gcgaggtcgc agagctcggt cactcgggct cctcgacgat agggggaacc
    16501 ttgaagaagt ccccctcccg gtcgggcgca ttggccaggg cttcgtcgcg gccgaggccg
    16561 tcccgcggct cgtcgggccg gaaaacgttg gccatcggga tcgggtgcgc ggtcggcggg
    16621 acggcctcgg ccgcgacctc ggtcacccgt tcggcgtgct ccaggatgac cccgagctgc
    16681 tcgcggaacc gctccagctc ctcgggggtc agtcccactc gcgccaagcg cgccacgtgc
    16741 tcgatgtcga tctcgaccgg catggcgccg agtctagcga accgtgaggt ggcggccggc
    16801 cgaaggcgct ggccgcgcgc atggctatcc tcgccgacgg caaccgccgc ctaggttcga
    16861 cccccgccgg ctcagaggcc gaggcgccta cggaactctt cctcgtcgat gatctcgacc
    16921 ccgagtgaga cggccttgtc gtacttcgac ccgggatcgg tccccgccac cacgaagtcc
    16981 gtgctcttcg agacgctcga ggtcacgcgc gcccccgcgg cctcggccgc cttcgttgcc
    17041 tcgtcccggc tcatggactc cagcccgccg gtcagcacca cccgcttccc cgccagcggc
    17101 ccgtccgggg tcggtccccc gggctcgggg tcctccatcc gcacgccggc cttcctcagc
    17161 ttcttcagca gccgcttcga ctccgggcgg cggaaccact cgtggacgct gcgtgcgatc
    17221 tcgggaccga tcccctcgac cgcctcgatc tcctccatct tcgccgctgc caaccgctcg
    17281 gtcgagggga aagcccgcgt cagcatccgg gccaccgtcg tccccacgtg gcggatgttg
    17341 agccccacga gcaggcgcca gatcggccgg tccttcgacc cctcgatcgc ccccagcagg
    17401 ttcccgatcg acttctcctt gaacccgggg agctccgcga gctgctcctc ggtgatgaag
    17461 tacacgtcgg ccgggtcggc cacccatccg cgatccatca gcgccatccc ggtcttgtag
    17521 ccgaggtgct cgatgtccat ggccccccgc ccggcgaagt ggaacagcca ctcgatgttc
    17581 tgcgacgggc acccctcgcg gttggggcag cgccagtcgg cctcgctttc ctcccgcacg
    17641 agcggcgtcc cgcaggaggg gcactccttg gggaacttcc agcgccgcgc cccccgcttc
    17701 cgcttctgca tcacgggccc gaccacctcg gggatcacgt cgccggcgcg ccggacgatg
    17761 acgtggtcgc cgggccggac gtccttccga gccacctccg actcgttgtg aagggtcgcg
    17821 gtcgtcaccg tcaccccgcc cacgaacacg ggctcgagct gggcgaacgg ggtcaccttc
    17881 cccgtccggc ccgtgtgcgt gaagatcccc cgcaggagcg tggtccgctc ctcgggcggg
    17941 aacttgtagg cgatggccca gcgcggggcc ttcgcggtgg ctcccagctc ctcctggagc
    18001 gagatctggt ccaccttcac gaccacgcca tcgatctcgt agtccacgtc gtgacgcccc
    18061 tcctgccact tctcgcagaa ggcgaagacc tcctcgaggc tgtccagcac ggcgttgtcg
    18121 gggttcacgg gcagcccggc ctcccgcagg tgatcgagcg cctcggagtg ccgcgagaac
    18181 cgccgcccgt cggcccagcc gagcccgtgg acccagagcc gcagcggcct gcgagccgtc
    18241 acgccgggat ccttctgccg gagtgagccc gccgcggcgt tccgcgggtt ggcgaacgcc
    18301 cgcccgcccg cctcctgcag ctcgcgattc atctcctcga aggccttcac ggggaggtag
    18361 acctcgccgc gcacctcgag cacccccgag tgctcgccgc gcaggcgcat cggcgccccc
    18421 cccaccgtcc ggatgttcgc ggtgatgtcc tcgcccgtgt atccgtcgcc tcgcgtggcc
    18481 ccgcggatgt agcggccgtc ctcgtaggtc agcgccaccg cgacgccgtc gatcttcagc
    18541 tcacagacga agtccccggc ccgccccacg gctcgctcca ctcggcggcc ccactcgctg
    18601 agctcgtccc aggtgaaggc gttgtcgagc gagagcatcg gggtacggtg ctcgacgggg
    18661 gcgaagagct cggagacctg gccgcccacg cgctgggtcg ggctgtcggg cgtgacgaac
    18721 tcggggaact ccgtctccag cttctccagc tcgcggacga gctcgtcgta ctcggcgtcg
    18781 gagacctcgg ggtcgtcctc gacgtagtag cggtactggt ggtggcggat ctgctcgcgc
    18841 agctcctcga tccggagccg gacgtccgag cgccgtcgtt cctcgggttc gcggcggtcc
    18901 tcgctcatcg ggggacagga tagtcagagc cggccggcgg cgatgatccg ctggaggaac
    18961 cggcgcgtgc gggggtgctc gggctcggcg aacatcctgt cggggggacc ctcctcgagg
    19021 atcacgccct cgtccaggaa gctcacccgg ccggcgatgt ccttggcgaa ccccatctcg
    19081 tgcgtggcca ggatcatcgt catgcccgcc tcggccagct cgcggatcac gtcgagcacc
    19141 tcggcgatca gctcggggtc gagcgcgctc gtcacctcgt cgaggaggag gaggtcgggc
    19201 tgcatggcca tggcccgaac gatggcggca cgctgctgct ggccccccga cagacggtcc
    19261 gggtactcgt cgcgcttgtc gaggaggccg aagcgcgcga ggagctcctc cgcgcgcgct
    19321 tcggcctcct cgcgggacag ccccagcgcc tcgatcggtc ccaaggtgac gtttcgcagg
    19381 accgtcatgt gagggaagag gttgaaggcc tggaacacga tcccgatccg ctttcggacc
    19441 tcgttctcgt tcacgctggg gagggtgatc tcgtcgccgc tcaggaggat gcgcccggca
    19501 tcgatcggct ccaagaggtt gacacagcga aggagcgtgg acttgcccga ccccgaggcg
    19561 ccgatcagtg cgatcacttc gtgctggtcc accgacaggt ccacgccccg gagcacgtcg
    19621 accttcgcga aggacttgtg gacgccctcg agggtcaagg cccgggtcac agcgcgctcc
    19681 ccgcctggtg gcgccgccgc tcccgcgcga tcatccagtc cgtgaaccgg gccagcggga
    19741 tcgtgatgag gacgaacagc agcgcggtcg ccaggtacgg cgtgtagttg aagttgccgg
    19801 ccgagtccac ctgggactgg cggaacgcct ccagcacccc gatcagcgcg agcagcgccg
    19861 tgtccttctg gaggccgacg aagtcgttca gcagcggggg gacgacgcgg cggaccgcct
    19921 gcgggaggac gacgaaccgc agaccctgcc accgggtcag cccgagcgac cgcgccgccg
    19981 ccacctggct cgggtgcacc gactcgatcc ccgcgcggta gacctccgcc acgtaggccg
    20041 agtacacgag cacgagggcc acgatccccc agaagaaggg gtccttgggg atccggagct
    20101 gcagcgcggg ggccccgaac cccaggatgt acatcaccag gatcgtcggg atgcctcgga
    20161 acaagtccgt gtagccgacc gcgaggagac ggatcgggag gaagaccggc cccgggaggc
    20221 tccgcagcac cgcgatgaac atcgccagga cgaggatgaa gacctcggcg atcaggaagg
    20281 ccgcgacgtt cttgaggaac ctcctcgaga tgtcggggaa cgattccagg aagatctcgg
    20341 cgttgaagaa cgaccgccgg acctcgggcc acccgggcga gttgatcacg agcagcacga
    20401 gcaccgtgaa gaagatcagg gtgctgacga ccgcgatcgg gacgctgtag cgcccctccc
    20461 agtgccctcc ccccaggatg cgcgagcgac gatcgccggg aggcatcggc ggcgccgggc
    20521 tcccgggcgg ggtcgtgacg ctcatctcct ccgccggagg cctactcgag gaccggcgcg
    20581 tcggtcttgt cggccagcca ctcttgctgg atcgagtcca gcgtcccgtc ctcgcggagc
    20641 gcgtccaggg cctggttcac gcagtcccgg agggggttgc cctcctcgaa caccatcccg
    20701 aagtactcct gctgcccgac cgtcgggaac tgccccacga tcgtcccgtt gtcgagctgg
    20761 acggccacga tgaagaacgc ggtcggaagg tccaccacga tcccgtcgac ctgaccgttc
    20821 tccaggccgc tgatcgagtc gttcagcgtg tcgtagacgc gagggtcgct gtcgggctgg
    20881 atgttctggt cgatgtactc gaagctcgtt gtccccacgg gggctccgag cgtgtactgc
    20941 ttcagctcct cgaccgtact ggcctccgcg atcggcgtgt ccgccaccgc cacgagcgcc
    21001 tggttcacgt cgtagtagga gtcgctgaag tccaccgcct cgtcgcgccg aggccggtac
    21061 gagacctggt tgatgtcgaa gtcgaagtcc ttcggtcctg gagcgaacgc ctggttgaag
    21121 gggacctcga cccactccac ctcgtcctcg ccgaacccca gctcctccgc caccgcgtag
    21181 gccaccgcgc tctcgaagcc ctcgccggtc gaggggtcgt tgatctccca cgaggatccc
    21241 tcgggcgtcc cgccctcgaa ccacggcggg aaggcggggt tgtcggtccc gatcgtcagc
    21301 tggccggggt tcacgagggt gaggttctcg gtggcgcact cgtccaccgt gggcgagctc
    21361 gtcggagccg cctccggcgt ctcgtcctct tcctcgggcc cgcacgccgc aagcacgagc
    21421 ccggagatca tcaggacggc agcgatcttg cgcatcgagc gtctccccct cttccctgcc
    21481 ggccactcga gccggtcccc gatggcgcaa tgtagcaccc tgtggatcgg aatgcggagc
    21541 ggccccggcc tcccggccgg ggccgctccg acaccgctgc ttgccggatc aggtcgtgcc
    21601 ggggagcacc atcagcagga gctgaccgtt ctcccgcttg taggtgacgt cgccgatcac
    21661 ctccgacgac tcgaaggagc cgtgggcctg gacgtccagg agccacgccc cgtccccgaa
    21721 gaacgcggag gcatccacga tcccgctgga ctccccgtcg ggatcatcca ccgtcgccac
    21781 gtgcgtccag gatccggacg cgaagtcgtg ggcccagacc ttcgcgtcgt cggcgtcctc
    21841 ctgcaccatc agggacttcc cgctcgtgtc gatgttgtcg ggcgcccgca tcggcacgaa
    21901 gacgtcggtg ccctcccagt cgccgtccgc gtacacggtc agggagttca ccttccgcgg
    21961 gttgctcttg ttcatcacca tcttgaatat gcggccgttg tcggcctcgc ccacggtgcc
    22021 gctcgggccc cgcaccatcc ggccggtgtc cgggtcgggc acgacccggc tcgcacccgt
    22081 gtcggcgatg tacacgacgc gggggttgtg gcgatcgtag gccaggtcct ccagcctgat
    22141 gaactggaag acgttgtgct gattcgacca gcgctccagc gcctcctgcg gcggaagcgc
    22201 cgtcctccca cgggcgatcc gcttcggcac gcggatgaac cgacccttga tctcgtcgcc
    22261 ggggtcgagg tccaggtagt cgttcgcccc gttgaagggg ttcccgggat tcacgcggct
    22321 cccgttcttg gccgtggcct ggaaccccca gaggctcccc ttgtcccgcc agatgtgtcg
    22381 ctcgcggttg gccaggtaca tgtagagctg cgacgtgggc gggctgaacg tgtcgtcggt
    22441 ggacaggatc gcgatctgcg gtccagcgcc cttcccggca cgacccttct tcttgccctt
    22501 ccctcgctgg ctccagccgc ccgggatcac cacgctgttc tcgtgattgt ggcgtcccat
    22561 cccggcgacc tgggtgaacg ccccggtctc ggcgttcagg acgaccgcat agccggcctg
    22621 gcgctggggg gcgacggcgg gatccgggcc gtacggggcg cccgacggca cgtcgatgat
    22681 gtcgctcgac tcctcattgg cgaagaacgt gtaggtcttg aagccctcgg cggggcccgc
    22741 catgaacgag gagcagaagc ggatgtagcc gttctcgggc ccgatcggga ccgacgcctc
    22801 caagacgccg gcgtcgctcg tgtcgagggt gagctgggac accgaggcgt cctcgaagtc
    22861 ggcgaacccg aggaacggaa ccgtggactg ctcgtgggag acgaagacct cgacttcgcc
    22921 cggcgctccg ggcatcaagc cgatcccgtc cgggatcccc tcgaacgtga agtctccgat
    22981 cgtgtcgccg ctcgagatga tcgcgacgac cgaagagccc ggtggagcgt caccggtgag
    23041 ggacagcatg ggtggaagcg cggtatggaa cccgtccgcc tgagccgggg cggcgacccc
    23101 tagggccagt acgagcgcga ccgggacgga ccatcggcgt ctagatctca tgtctctcct
    23161 tccggataga ggatgcccac cattctcccc cccgcgaacg agcctaagtc aaatgacgga
    23221 tggggcccgc tcagggttgg cgtcgggctc agcggagcga gcgggcgatc gcgccgccgc
    23281 cgagcagctc atcgcccgcg tagaagacaa cgctctgtcc cggcgcgatc gcccgctgcg
    23341 gcgcccggaa cgtcacgcgg acctcgcctc cggggagcgg ctcgaccacg gcggcggccg
    23401 cctcgccgtt gtaccggatc tgagcctcgg cctcgatggg cccgtccgga gcatcggcga
    23461 cccagctcac tcgctcggcg atcagtcccc gcctggagag cagctcgccg gggccgacca
    23521 cgacccggtt cgcgcccgcg tcgacgtcca ggacgtaggc tcgctcgcct gtggagacgc
    23581 ccaacccccg gcgctggccg atcgtgaagc ggtgggcccc ggcgtgcgtc ccgaggacgc
    23641 ggccgcccgc gtccaccatc tcgccctc
//
LOCUS       DASUTZ010000257         5614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1912789, whole genome shotgun sequence.
ACCESSION   DASUTZ010000257 DASUTZ010000000
VERSION     DASUTZ010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1912789"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..444)
                     /locus_tag="VF097_11025"
     CDS             complement(52..444)
                     /locus_tag="VF097_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011944009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEX6263366.1"
                     /translation="MMDGGGERPPLRRSRTDRIVGGVAGGIARYFGIDPILIRLGFIL
                     LTIAGGSGVLIYLIAWVIIPEEEEGAERERAGPAMDAATTRLLLGGALIAIGGILLAS
                     RFIPYFSRVFWPLVLIVAGAAVIMGARR"
     gene            complement(450..1142)
                     /locus_tag="VF097_11030"
     CDS             complement(450..1142)
                     /locus_tag="VF097_11030"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEX6263367.1"
                     /translation="MGGISPARWLLGLFLVALGVLFLLDTTDLIEAAGFWSLAWPTLL
                     VFVGISILVRERWRSIVGLIVLLLGLGFFAQNAGWLEEGWIGRYWPIVLILAGIGVLV
                     EAMGFVRPRARLAETGETGDEGTYTAILSGRKSRVTSAAWRGGRATAILGGVELDLRD
                     AVPVSGGAVLDVTAFMGGVDVTVPRGWRVEIAGTPLLGGFDDKTDGGGGPEAPLLRIT
                     GTAIMGGVDIKH"
     gene            1309..2358
                     /locus_tag="VF097_11035"
     CDS             1309..2358
                     /locus_tag="VF097_11035"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX6263368.1"
                     /translation="MRGRTSTHIGAVALAGALLASLLTGPVGAQNTKEKLEDARARLA
                     EVESEAQAATAEYEAARGRLIQTEDEIQATRQRLEAVAKRANRLQTRLGERASEAYKM
                     GASGMLDLLFVSDSLSEFSDRMVFLDRIAQEDADLVLRADVLAERLERYEADLDALLK
                     RQAETTKILREKRELIYEKLEEAQAIKAKLEDKLAAEEAAARAAAQAASRSAPGSTDV
                     VVGGALQACPVPGSTFVDSWGAPRSGGRSHQGVDMMAPYGTPVYAATAGSMTQSSSSL
                     GGNQAYVQAPNGDVTFYAHLQGYEGGPRGVSAGELIGYVGDTGNATGTPHLHFEYHPG
                     GGAAVNPYPYVAAVC"
     gene            2422..2961
                     /locus_tag="VF097_11040"
     CDS             2422..2961
                     /locus_tag="VF097_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aerial mycelium formation protein"
                     /protein_id="HEX6263369.1"
                     /translation="MSDAPMAGGKRRIDRILADDFVRDLESLETDDLRARRDLSKAER
                     EYLSFLRRLLQGRRDILRDELRRREEGGEAGPLVERITAVLGEGPRGRSRGEAVSVPL
                     PEEEATLARRRIERLLSDASLSDLEGLSDDDLRETIERVEREERTVSEARGRVMGVHD
                     RLQGEMKRRLRAQLKGLRA"
     gene            3140..3856
                     /locus_tag="VF097_11045"
     CDS             3140..3856
                     /locus_tag="VF097_11045"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX6263370.1"
                     /translation="MDEQQVRDQLVVFAREIREIYRKERERSAELAHALDQLNDSYLS
                     TMKTLAFLVEAKDVGTRMHLDRTSQYGMALARLVAPGLAERPDLEWGFLLHDIGKIGI
                     SERILNKAGPLTTSEWSVMKTHPLIGAQVVAPMAFLGRASDVIRCHHERWDGRGYPRG
                     LKSEEIPLSARIFSVVDAFDAMTSDRPYRRALSVADAVAEISRCSGAQFDPDVVEAFL
                     ILMSGRTEPVEARPLLAPTG"
     gene            3873..4802
                     /locus_tag="VF097_11050"
     CDS             3873..4802
                     /locus_tag="VF097_11050"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX6263371.1"
                     /translation="MPVPLARVIRSGIEESIHLGSVAVADVEGRVVVSAGEADRLTYL
                     RSAWKPLQAAACVARIGGLHDREIAVMAGSHNAEEVHLEAVRSLLGRAGLDEGALGCP
                     PSRPIDPEPRSESPATILHDCSGKHAGMLLACVRAGWETGSYLDPAHPLQREIVELTG
                     AAAGAPVSSLGVDGCGAPAAALPLRAIATAFARLPQELGPAATRVVEAMRAEPYLVAG
                     RGRVCSAVMEAFPVVAKVGADGVAGASLVGRGLGIALKVDDGAGRARDAALVRALALL
                     DVVDPSRVPAFAAPPVLGGGTPVGELRSDFSLA"
     gene            4880..5254
                     /locus_tag="VF097_11055"
     CDS             4880..5254
                     /locus_tag="VF097_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016578749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX6263372.1"
                     /translation="MRTSVGRFIRDQRRQAQLSLRRLAQLAGVSNPYLSQIERGLKKP
                     SAEILQGIAKALRISAETLYVKAGILETEKAGDTVSAIMTDPALTERQKQALVQIYES
                     FRSETQARREERRMKTQRKEQT"
     gene            5251..5565
                     /locus_tag="VF097_11060"
     CDS             5251..5565
                     /locus_tag="VF097_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263373.1"
                     /translation="MIEQMQKAGYALIGLGDLAAEKVREMVGRARKVKVDRKEIESTY
                     GDLSKRGSKIVRKVTTSKPAKQAKEGTKQASRQLKGAATSLRKAAGLEEEEKKARAKK
                     AS"
BASE COUNT          854 a         1971 c         2048 g          741 t
ORIGIN      
        1 gcaggatcgc tccgaagacg agccggccga cggggatccc gcgctcctcg gtcatcgcct
       61 cgctcccatg atcacggccg cgccggccac gatgaggacg agcggccaga acaccctcga
      121 gaagtagggg atgaagcgcg aggccagcag gatccccccg atcgcgatca gcgcgccgcc
      181 cagcaggagc cgggtggtgg ccgcgtccat ggccggcccc gcgcgctccc gctcggcccc
      241 ctcctcctcc tcggggatga tcacccaggc gatgaggtag atcaggaccc ccgagccccc
      301 agcgatcgtg agcaggatga agccgagcct gatcaggatc gggtcgatcc cgaagtagcg
      361 tgcgatgccc ccggccacgc ccccgacgat ccggtcggtg cggctcctgc ggagcggtgg
      421 ccgctcgcct cctccgtcca tcatcccctt cagtgcttga tgtcgacccc gcccatgatg
      481 gcggtgcccg tgatcctgag caggggcgcc tccggccccc cgcctccgtc ggtcttgtcg
      541 tcgaagcccc ccaggagcgg cgtgcccgcg atctccaccc gccatcccct ggggacggtc
      601 acgtccacgc cccccatgaa cgcggtgacg tccagcaccg cccccccgga caccgggacg
      661 gcatcgcgca ggtcgagctc cacgccgccc aggatggccg tcgccctccc cccgcgccag
      721 gccgcgctcg tgacccgcga cttccgcccg ctcaggatgg cggtgtaggt cccctcgtcg
      781 cccgtctcgc ccgtctcggc caaccgcgct ctcggtctca cgaaccccat ggcctccacg
      841 aggaccccga tcccggccag gatcagcacg atcggccagt agcggccgat ccagccctcc
      901 tccagccagc ccgcgttctg ggcgaagaac cccagaccca ggaggagcac gatgaggccc
      961 acgatgctcc gccagcgctc gcggacgagg atcgagatgc ccacgaagac gaggagcgtg
     1021 ggccaggcca ggctccagaa gcccgccgcc tcgatcagat cggtcgtgtc gaggaggaac
     1081 aggacgccca gcgcgaccag gaacaggccg agaagccagc gagccggtga gatgcctccc
     1141 atgggaaccc cctcctccgc gtgcatcata gtcgcaggtc gggggcgggt tccaactcat
     1201 cgagggggcg tcgtggccct tgcctgactc gctgtttgaa gccccccggg acggctgata
     1261 gggtatcgcg tggccttggc cgacgcggcg accaggggga ggagtcgttt gcgaggaagg
     1321 accagcaccc atatcggggc ggtggcgctt gctggcgccc tcctcgcctc cctcctcacg
     1381 ggacccgtcg gcgcccagaa cacgaaggag aagctggagg acgcgagggc ccgcctggcg
     1441 gaggtcgaga gcgaggccca ggccgcgacg gcggagtacg aagcggctcg cggccggctg
     1501 atccagacgg aggacgagat ccaggccacg cgccagcgcc tggaggccgt tgccaagcgg
     1561 gcgaaccgcc tccagacgcg cctcggcgag cgcgccagcg aggcctacaa gatgggcgcc
     1621 tcggggatgc tcgacctcct cttcgtctcc gactcgctct cggagttctc cgatcggatg
     1681 gtgttcctcg accgcatcgc ccaggaggac gccgacctcg tcctgcgggc cgacgtcctc
     1741 gccgagcgcc tcgagaggta cgaggccgac ctcgacgccc tgctgaagcg gcaggccgag
     1801 accacgaaga tcctccgtga gaagcgggag ctcatctacg agaagctcga ggaggcccag
     1861 gcgatcaagg ccaagctgga ggataagctg gctgccgagg aggcggccgc ccgagcggcg
     1921 gcgcaggccg ccagccgctc ggcgccgggc tcgaccgacg tggtcgtggg cggcgccctc
     1981 caggcctgcc cggtcccggg ctcgaccttc gtggacagct ggggcgcgcc ccgctcgggc
     2041 ggccgcagcc atcagggcgt ggacatgatg gccccctacg gcacgccggt gtacgcggcg
     2101 acggccggca gcatgaccca gtcctcgagc agcctgggcg ggaaccaggc gtacgtgcag
     2161 gccccgaacg gcgacgtgac cttctacgcc cacctccagg gctacgaggg cgggcccagg
     2221 ggcgtctccg cgggcgagct gatcggctac gtcggcgaca ccgggaacgc gacgggcacg
     2281 ccgcacctcc acttcgagta ccacccgggc ggcggggccg ccgtcaaccc ctacccgtac
     2341 gtggcggctg tctgctgagg tctgttcccc cgctccgatc cgggcgataa tgcgagccgt
     2401 ggccccctgg aaggagccct cgtgagcgat gccccgatgg cgggcggcaa gcgccgcatc
     2461 gatcggatcc tggcggacga cttcgtccgg gacctcgagt cgctggagac ggacgacctc
     2521 cgcgcccggc gtgatctgag caaggcggag cgcgagtacc tctcgttcct ccgccggctc
     2581 ctgcaggggc ggcgcgacat cctccgcgac gagctccgtc gtcgggagga ggggggcgag
     2641 gccggtccgc tggtcgagcg gatcaccgcc gtgttggggg agggcccgcg ggggcgctcc
     2701 cggggcgagg ccgtgagcgt cccccttccc gaggaggagg ccacgctcgc caggcggcgc
     2761 atcgagcggc tcctctcgga cgccagcctg tccgacctcg agggtctctc cgacgacgac
     2821 ctgcgcgaga cgatcgaacg ggtggagcgg gaggagcgca ccgtgtccga ggccaggggg
     2881 agggtcatgg gcgtgcacga ccgcctgcag ggggagatga agcggcgcct ccgcgctcag
     2941 ctcaagggac tccgggcctg acgccgtccc ccgaccgggg gatgcgcgca aatcaccgga
     3001 ccgcgggatg tgccgcccgc ctgggtgatg ggaccattcg atcaggcgat cctccgaggc
     3061 tcccaggaga ccggggatcg cgggcttata ctgcggcccc gacgcgccgc cgggcgcggg
     3121 cgggaggccg gggggttccg tggacgagca gcaggtcaga gatcagctgg tggtcttcgc
     3181 ccgggagatc agggagatct accggaagga gcgggagcgc tccgccgagc tggcgcacgc
     3241 cctcgaccag ctgaacgact cgtacctctc cacgatgaag acgctggcct tcctggtgga
     3301 ggcgaaggac gtcgggaccc gaatgcacct cgaccgcacc agccagtacg gcatggcgct
     3361 ggcgaggctc gttgctcccg ggcttgccga gcgccccgac ctggagtggg ggttcctcct
     3421 ccacgacatc ggcaagatcg ggatttccga gcggattctc aacaaggcgg gccctctcac
     3481 gacgtccgag tggtccgtga tgaagacaca cccgctgatc ggcgcgcagg tggtcgcccc
     3541 gatggccttc ctcgggcggg ccagcgacgt gatccgctgt caccacgagc ggtgggacgg
     3601 tcgaggctac ccccggggtc tcaagagcga ggagatcccg ctctcggctc ggatcttctc
     3661 ggtggtggac gcgttcgacg cgatgacgag cgatcggccg taccgacgcg ccctctccgt
     3721 cgcggatgcg gtggcggaga tctcccgctg ctcgggggcg cagttcgacc ccgacgtggt
     3781 cgaggcgttc ctgatcctca tgagcggcag gaccgagccg gtcgaggctc gccccctgct
     3841 cgcccccacc ggctgacggc tacgctgtcc ccgtgccggt tcctctcgcg cgggtgatcc
     3901 gttcgggcat cgaggagtcg atccatctgg gatcggtcgc ggtggcggac gtggagggtc
     3961 gagtggtcgt ctccgcgggc gaggccgatc gcctcacgta cctccggtcg gcgtggaagc
     4021 cgctccaggc cgcggcgtgc gtggcgagga tcggcggcct ccacgaccgg gagatcgcgg
     4081 tcatggcggg ctctcacaac gccgaggagg tgcacctcga ggcggtgcgg tccctgttgg
     4141 ggagggccgg gctggacgag ggggccctgg gatgcccgcc gagccggccg atcgatcccg
     4201 agccccgttc ggagtcgccg gcgacgatcc tgcacgactg ctccggcaag cacgcgggca
     4261 tgctgctggc gtgcgtgcgc gccggatggg agacgggctc gtacctcgac cccgcgcatc
     4321 ccctccagcg ggagatcgtg gagctcacgg gggcggcggc cggcgcgccg gtctcctcgc
     4381 tgggcgtgga tgggtgcggc gcgccggccg ccgccctccc tctgcgcgcg atcgccacgg
     4441 ccttcgcccg cctcccccag gagctggggc ccgcggcgac ccgggtggtc gaggcgatgc
     4501 gagccgagcc ctacctcgtc gcgggtcggg ggagggtgtg ctcggccgtg atggaggcct
     4561 tcccggtggt ggcgaaggtg ggggccgacg gcgtggccgg ggcctcgctc gtgggccgcg
     4621 gcctggggat cgcgctcaag gtcgacgacg gcgcggggcg ggctcgcgac gccgccctcg
     4681 tccgcgcgct ggcgctcctc gacgttgtgg acccgtcccg tgtgccggcc ttcgccgccc
     4741 ctccggtcct cgggggcggg accccggtgg gggagctccg ctcggacttc tccctcgcct
     4801 gaggtgcttg ccagcggttc ccgctttgct tgccatagtt agcacttccg tgatagggtg
     4861 cagatggaag gagacgggga tgcggacgag cgtgggacgg ttcatcaggg accagcgccg
     4921 gcaggcgcag ctgtccctga ggaggctggc ccagctggcc ggcgtctcca acccgtacct
     4981 ctcgcagatc gagcgtggcc tgaagaagcc gagcgccgag atcctccagg ggatcgccaa
     5041 ggcgctccgg atctccgccg agacgctgta cgtgaaggcc gggatcctcg agaccgagaa
     5101 ggccggggac accgtgagcg cgatcatgac cgacccggcc ctcaccgagc ggcagaagca
     5161 ggccctcgtc cagatctacg agagcttccg atcagagacg caggcgcgcc gcgaggagcg
     5221 gcgcatgaag acccagagga aggagcagac atgatcgagc agatgcagaa ggcagggtac
     5281 gcgctgatcg gcctcggcga cctggccgcc gagaaggtcc gggagatggt gggccgcgcc
     5341 cgaaaggtca aggtcgaccg gaaggagatc gagtccacct acggcgatct cagcaagcgc
     5401 ggctcgaaga tcgtgcgcaa ggtcacgacc tcgaagccgg cgaagcaggc gaaggagggc
     5461 acgaagcagg cctcgcgcca gctgaagggc gccgcgacca gcctccggaa ggccgcgggc
     5521 cttgaggagg aggagaagaa ggctcgggcc aagaaggcca gctgaggccc agcgcctgga
     5581 ccggggcggc cgccgcgcgc gaacgcgcgc ggcg
//
LOCUS       DASUTZ010000258         2805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_488090, whole genome shotgun sequence.
ACCESSION   DASUTZ010000258 DASUTZ010000000
VERSION     DASUTZ010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488090"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1419
                     /locus_tag="VF097_11065"
     CDS             <1..1419
                     /locus_tag="VF097_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX6263374.1"
                     /translation="EVADAPYLHRGLNLADMAHVLVLLEQGIAPPEPAQRLLRLLLEV
                     HGTPHEDFPYDPSLGDAYNSRERFFIERLGTDAGWLHAGRPRREAGRVALRICLRDLV
                     VSLVSDAAGFAESLADAAEEHADVVMPDQTYLQQAQPSTFGHYLLTFAGPSLRESDRL
                     LLEDDWVNRSPGGAGCVNGSRLAVDRDRTASLLGFRGVIEHTRDAMWQTDGLVALLSN
                     STSLVSNLSKLAEDLEIWDSQEFDFVELAGPYTRSSILMPQKRNPYALSIVRGASGVL
                     IGRLSGFLAVVKTPSARSDNLIFAYGEVPRALELAGRVTRLMRGVVSTMRPHPERMWE
                     SLTQGFSQATDLAEHVMQTAEVDYRTAYLVVGEAIQRAAREGLRGMDVTGELLDEAAR
                     EVVGRPLGLAERDLSDVLDPRAIVRTRTSRGGSAPEVVRDMAAGFRAEAERVRAEAAR
                     RAEAYARAEADLLSLARRTAGG"
     gene            complement(1388..1819)
                     /locus_tag="VF097_11070"
     CDS             complement(1388..1819)
                     /locus_tag="VF097_11070"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX6263375.1"
                     /translation="MEVTAYWEGGYTTRVPIRDFEIRIDEPERYGGRDTGPMPTEMLL
                     GTLAACFAMAFHHAAKKDDVALPDLEVTARGEYGNLRFDRIAVEVRTSHPDREQLEAF
                     LEKAIEYCYVTNTLAGSPELEYHVAGGEAHEVSPPPSAERG"
     gene            1818..>2805
                     /locus_tag="VF097_11075"
     CDS             1818..>2805
                     /locus_tag="VF097_11075"
                     /inference="COORDINATES: protein motif:HMM:NF018275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HEX6263376.1"
                     /translation="MTVESSNSEPLALPEEVHAVNVGLAVFGEAVRAQGGEAVDVEWR
                     IPAGGDRESVEALTALYGPRAAEIDEANEEVLRRIDGSAPVLVGVGSAGRDVPDMGER
                     AILHPGPPLPWEEFCDPLRRSVHAAVMAEGWAETPEEAGKLVAGGDVELDAANHHATV
                     LPMATTIGPTAPVLLMESPQGGTPAFAAINQGPGDQQWLGVDRPEAVERLVFLREAAA
                     PVLDAAIRAGEPLDVFSIAAQALQMGDDAHMRSQAATNLTIRFLLPRLLRLDDERAPE
                     VGTFLSENHLFFLTVGMAAAKATMLWAEQVEGSSIVTGMARNGTTFGVRLPGL"
BASE COUNT          365 a          974 c         1059 g          407 t
ORIGIN      
        1 gaggtcgccg acgcgcccta cctgcaccgc gggctgaacc tggccgacat ggcgcacgtg
       61 ctcgtgctgc tggagcaggg gatcgcgccc cccgagccgg cgcagcggct gctgcggctg
      121 ctgctcgagg tccacggcac gccccacgag gacttcccct acgacccgtc gctgggcgac
      181 gcgtacaact cccgggagcg gttcttcatc gaacggctgg ggaccgacgc ggggtggctg
      241 cacgccggcc gcccccgccg cgaggctggg agggtcgccc tccgcatctg tctgcgggac
      301 ctggtggtct cgctcgtctc ggacgccgcc gggttcgccg agtccctggc cgacgcggcc
      361 gaggagcacg ccgacgtcgt gatgcccgac cagacgtacc tccagcaggc ccagccctcg
      421 acgttcgggc actacctctt gacgttcgcc ggcccatcgc tccgggagtc ggaccgcttg
      481 ctgctcgagg acgactgggt gaatcggagc ccgggagggg cgggctgcgt gaacggcagc
      541 cggctggcgg tcgaccgcga ccggacggcc tcgctcctcg gattccgagg cgtgatcgag
      601 catacgcggg acgcgatgtg gcagacggac ggcctcgtgg cacttctctc gaactccacg
      661 agcctcgtca gcaacctcag caagctcgcc gaggatctgg agatctggga cagccaggag
      721 ttcgacttcg tggagctggc cggcccctac acgcgatcca gcatcctgat gccgcagaag
      781 cggaacccct acgcgctgtc gatcgtccgc ggcgcctcgg gggtgctgat cgggcggctg
      841 tcgggcttcc tggccgtggt gaagacgccc tcggcccgca gcgacaacct gatcttcgcc
      901 tacggggagg tcccgagggc cctggagctc gcggggcgcg tcacgcggct gatgcgcggg
      961 gtcgtgtcca cgatgcgccc gcaccccgag cggatgtggg agtcgctgac gcagggtttc
     1021 tcccaggcca cggacctcgc cgagcacgtc atgcagacgg ccgaggtcga ctaccggacg
     1081 gcctacctcg tggtgggcga ggcgatccag cgagcggcgc gggaggggct tcgggggatg
     1141 gacgtcaccg gggagctgct cgacgaggcg gcccgggagg tggtgggcag gccgctgggc
     1201 ctcgccgagc gggacctgtc ggacgtgctc gatcccaggg cgatcgtccg gacgaggacc
     1261 tcgcggggcg ggtcggcgcc ggaggtggtc cgggacatgg cggccggctt ccgggccgag
     1321 gccgagcgcg tgcgggccga ggcggcgcgg cgcgccgagg cctacgcccg ggccgaggcc
     1381 gacctgctca gcctcgctcg gcggacggcg gggggctgac ctcgtgcgcc tctccgccgg
     1441 ccacgtggta ctcgagctcc ggactcccgg cgagcgtgtt cgtcacgtag cagtactcga
     1501 tcgccttctc caggaacgcc tcgagctgct cgcggtcggg atggctggtg cgcacctcga
     1561 ccgcgatccg atcgaagcgg aggttgccgt actcgcctcg ggccgtgacc tccaggtcgg
     1621 ggagcgcgac gtcgtccttc ttggccgcgt ggtggaaggc catggcgaag catgcggcca
     1681 gcgtgcccag cagcatctcg gtgggcatgg ggccggtgtc acgtccgccg taccgctcgg
     1741 gctcgtcgat cctgatctcg aagtctcgga tcgggacccg cgtcgtgtat ccgccctccc
     1801 agtaggcggt aacctccatg acggtggagt ctagcaacag cgagccgctc gccctcccgg
     1861 aggaggtcca tgccgtcaac gtcggcctgg ccgtgttcgg cgaggccgtg cgggcccagg
     1921 gcggggaggc ggtggacgtg gagtggcgca tccccgccgg cggcgaccgg gagtccgtgg
     1981 aggcgctgac cgccctctac ggtcctcggg ccgccgagat cgacgaggcg aacgaggagg
     2041 tcctgcggcg catcgacggg tccgcccccg tcctcgtcgg cgtcgggtcg gccgggcgcg
     2101 acgtgccgga catgggggag cgggcgatcc tccacccggg ccccccgctc ccgtgggagg
     2161 agttctgcga tcccctgcgc cgctcggttc acgcggcggt gatggccgag gggtgggccg
     2221 agaccccgga ggaggccggg aagctcgtgg cggggggcga cgtggagctc gacgccgcga
     2281 accatcacgc caccgtgctc ccgatggcga cgacgatcgg gcccaccgct ccggtgctgc
     2341 tcatggagag cccccagggc gggacccccg ccttcgccgc catcaaccag ggtcccggcg
     2401 accagcagtg gctgggcgta gatcgtcccg aggcggtgga gcggctcgtc ttcctccggg
     2461 aggcggcggc tccggtgctc gacgcggcga tccgtgcggg cgagcccctg gacgtcttct
     2521 cgatcgccgc gcaggcgctg cagatggggg acgacgccca catgcggtcg caggcggcca
     2581 cgaacctcac gatccgcttc ctcctcccgc ggctgctccg tctcgacgac gagcgcgctc
     2641 ccgaggtggg gacgttcctc tccgagaatc acctgttctt cctgaccgtg gggatggccg
     2701 cggcgaaggc cacgatgctc tgggcggagc aggtcgaggg ctcgagcatc gtcacgggga
     2761 tggcgcggaa cggcaccacg ttcggggtgc gcctgcctgg gctcg
//
LOCUS       DASUTZ010000259         2699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_879651, whole genome shotgun sequence.
ACCESSION   DASUTZ010000259 DASUTZ010000000
VERSION     DASUTZ010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879651"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..877)
                     /locus_tag="VF097_11080"
     CDS             complement(50..877)
                     /locus_tag="VF097_11080"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014444510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX6263377.1"
                     /translation="MCLAGTAETVRKHRHEGPVLTRRRFLAAGGAAAALAAMPAPALA
                     RSTRHRHTRDLTHVFREGFPVYTGNSPSRQTLVTIPANGFYAQRWTFDEHTGTHLDAP
                     GHFIEGNRLADELPPDELVRPLAVVDISRRAAKNPDAEVRVKDLVAFERRHGRIPRRA
                     IVAMHSGWDARAGDRDSYLNPDGSGVFHFPGFSLEAVEWLLERRDVQGIGVDTSSLDF
                     GPSTTFIVHTVLLGEERYGLEGLANLGGVPANRATVFVGLIPWEEGSGGPCRVIARW"
     gene            1019..1201
                     /locus_tag="VF097_11085"
     CDS             1019..1201
                     /locus_tag="VF097_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX6263378.1"
                     /translation="MQKQFEGRWDQVKGKVKETWGDITDDELDQTEGKWEQLVGKIKA
                     KTGESQEEIERKLGSM"
     gene            complement(1278..1868)
                     /gene="cysC"
                     /locus_tag="VF097_11090"
     CDS             complement(1278..1868)
                     /gene="cysC"
                     /locus_tag="VF097_11090"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019499058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEX6263379.1"
                     /translation="MAEIFAKEGMRTSNAPKEGNKGFTLWMTGLSGSGKSTLAERLVP
                     IFDERGLSVELLDGDVIRTHLSKGLGFSKEDRDTNILRIGWVANRLSAHGAAVITSAI
                     SPYRAIRDEVRAGMPERFVEVFVSASVEECARRDTKGLYERAFKGEIANFTGVSDPYE
                     PPESPEILVDTERFSIQECLDQILARLEELGHLPAS"
     gene            complement(1868..>2699)
                     /locus_tag="VF097_11095"
     CDS             complement(1868..>2699)
                     /locus_tag="VF097_11095"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6263380.1"
                     /translation="HMVRTKPWDFFMMVEMGTDRLHHVFWQYCDPDHPKYEPGNPYES
                     AFVDYYRMLDGEVGALLETFPDDVAVILMSDHGARAMAGGVLFNDWLLQEGYLVMERP
                     LTEPTPIAKAPIDWSKTTAWGDGGYYGRLFLNVKGREPQGTIDPEDYERVRAEIAGKL
                     QEMKDPDGRPLGTRVFRPENVYPEVRGVAPDLIVYFGDLEWRSVGTVGTGETFTYEND
                     TGPDGANHDKRGVFALSNLPGVETGMVEAGFRLIDVGPTLMSLYDVPIPEGVEGRSIL
                     "
BASE COUNT          428 a          922 c          941 g          408 t
ORIGIN      
        1 gcgggtctga catgaaccgc tcgtcagacc cgcccgaaac gcggcgatcc taccagcgcg
       61 cgatcacccg gcaggggccg ccggagccct cctcccacgg gatcaggccg acgaagaccg
      121 tggcgcggtt cgccgggacc cccccgaggt tggcgaggcc ctcgaggcca tagcgctcct
      181 cccccagaag caccgtgtgc acgatgaagg tcgtggaggg gccgaagtcg agtgacgacg
      241 tgtccacgcc gatcccctgg acatcgcgcc gctcgagcag ccactccacg gcctcgagcg
      301 agaacccggg gaagtggaac acgcccgacc cgtcggggtt gaggtacgag tcccggtcac
      361 cggcccgggc gtcccaccct gagtgcatgg ccacgatcgc gcgcctgggg atcctcccgt
      421 ggcgccgctc gaaggccacg aggtccttca cccgcacctc cgcgtcgggg ttcttcgccg
      481 cgcggcgcga gatgtcgacc acggcaaggg ggcggacgag ctcgtccggc ggcagctcgt
      541 cggccagccg gttcccctcg atgaagtggc cgggggcgtc gagatgcgtc cccgtgtgct
      601 cgtcgaacgt ccaccgctgc gcgtagaagc cgttggccgg gatcgtcacg agcgtctgcc
      661 ggctcgggga gttgcccgtg tagacgggga atccctcgcg gaagacgtgg gtgagatccc
      721 gcgtgtggcg gtgacgggtg ctccgggcca gcgccggggc gggcatcgcg gcgagcgcgg
      781 ccgccgctcc ccccgccgcg aggaacctcc gccgggtcag caccggcccc tcgtgccggt
      841 gcttccgaac cgtctcagcg gtaccagcca ggcacatggc tccacctcct acagctcggt
      901 aggtcaagag cctacgcccg cgtccggggc cgggacaagc tgacgctcgc tccggtttct
      961 cagcccggac ccccgggtat ccagggggaa ccgacggaag gagcggacca tgggtggcat
     1021 gcagaagcag ttcgagggca ggtgggacca ggtcaagggc aaggtcaagg agacctgggg
     1081 cgacatcacc gacgacgagc tcgaccagac cgaagggaag tgggagcagc tcgtgggcaa
     1141 gatcaaggcc aagacgggcg agtcgcagga ggagatcgag cgcaagctcg gaagcatgta
     1201 aggcgccccc acgaggggca acgagggcgg cactctacga gcgggcgggg gtgccgctct
     1261 cctgcgtccg gggcggatca ggatgcaggc aggtggccca gctcctccaa gcgggccagg
     1321 atctggtcga ggcactcctg gatcgagaag cgttcggtgt ccacgaggat ctcgggcgac
     1381 tccggcggtt cgtaggggtc gctcaccccc gtgaagttgg cgatctcgcc cttgaacgcc
     1441 cgctcgtaga gccccttcgt gtcgcggcga gcgcactcct cgacggaggc cgagacgaac
     1501 acctcgacga agcgctcggg catccccgcg cggacctcat cccggatggc cctgtagggg
     1561 ctgatcgcgc tcgtgatcac cgcggcgccg tgcgccgaga ggcggttcgc cacccagccg
     1621 atccggagga tgttcgtgtc gcggtcctcc ttggagaagc cgagaccctt ggagaggtgc
     1681 gtgcggatca cgtcgccgtc caggagctcg accgagagcc cgcgctcgtc gaagatcggc
     1741 acgaggcgct cggcgagggt ggacttcccc gagccggaga gcccggtcat ccagagggtg
     1801 aagcccttgt tgccttcctt gggggcgttg gaggtccgca tgccctcctt cgcgaagatc
     1861 tcggccacta gaggatgctc cttccttcca cgccttcggg gatcgggacg tcgtacaggc
     1921 tcatcagggt ggggcccaca tcgatcaggc ggaaccccgc ttccaccatc ccggtctcga
     1981 cgcccgggag gttggagagc gcgaacaccc cccgcttgtc gtggttcgcg ccgtcaggtc
     2041 ccgtgtcgtt ctcgtaggtg aaggtctcgc cggtgccgac cgtccccacc gacctccact
     2101 ccaggtcccc gaagtacacg atgagatcgg gcgccacccc gcgcacctcc gggtagacgt
     2161 tctccgggcg gaacacccgc gtgccgaggg gccggccgtc cgggtccttc atctcctgga
     2221 gcttcccggc gatctcggcc cggaccctct cgtagtcctc cgggtcgatc gtcccctggg
     2281 gctcgcggcc cttcacgttg aggaagagac ggccgtagta gccaccgtcg ccccacgcgg
     2341 tggtcttcga ccagtcgatc ggggccttgg cgatcggcgt gggctcggtg agaggccgct
     2401 ccatgacgag gtagccctcc tgcagcagcc agtcgttgaa gaggacgccc ccggccatcg
     2461 cccgagcgcc gtggtcgctc atgaggatca cggcgacgtc gtctggaaag gtctccagga
     2521 gcgcgccgac ctcgccgtcg agcatccgat agtagtcgac gaacgcgctc tcgtaggggt
     2581 tgcccggctc gtacttcggg tggtccggat cgcagtactg ccagaagacg tggtggagcc
     2641 ggtcggtccc catctcgacc atcatgaaga agtcccaggg cttggtcctc accatgtgg
//
LOCUS       DASUTZ010000260         9144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_620640, whole genome shotgun sequence.
ACCESSION   DASUTZ010000260 DASUTZ010000000
VERSION     DASUTZ010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9144
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620640"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VF097_11100"
     CDS             <1..292
                     /locus_tag="VF097_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263381.1"
                     /translation="VQLAIQRKKNSGKCQWWNGKRFRGGKCKRVPPKFWKKAKYDRFL
                     DWYLYRVPSFKPTVGTNIRFYRAFARGFDHAGNVETRFQKKRNKNTFWIKRR"
     gene            841..1758
                     /locus_tag="VF097_11105"
     CDS             841..1758
                     /locus_tag="VF097_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263382.1"
                     /translation="MNDEGVEDAVESFNFVLSGATGFPPLSSNRPTEAAVHIIDNDGP
                     ARVSLEPTLDGGDTMQYEPGEQASHFHIPVFWAGETLPSGTVPYTIGPADGFPTPTLD
                     VDYEVNSPAQLPASDFGGGRVAFIKLRILNDNKEELDEAITITLTGANAVEGRDETTV
                     LIRDAGVDNLAPRTNFHHPKHKKRYPRNAYQLREMHIFHSDPGGSGIRRVQIAIQRRL
                     TNGRCQWWNGKRFAGGSCKGVPAKFWKKTKYERALDWFLYRVPAFKPTQGTNLRFYRA
                     FARGFDMAGNVERRFQKGRNKNTFWVKRS"
     gene            2004..4100
                     /locus_tag="VF097_11110"
     CDS             2004..4100
                     /locus_tag="VF097_11110"
                     /inference="COORDINATES: protein motif:HMM:NF014301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA domain-containing protein"
                     /protein_id="HEX6263383.1"
                     /translation="MSRRTRWLVLPITLVAVLALGIPPSSADHATRQDVKNLVPVADV
                     ENSVPFAINSDMAFWGDLAYQGNFEGFQIHDISDPENPVNLADVENCPDGQGDVFVWD
                     DLLVYSRNSPANNDTCDGVALPNGFEGVHLWDVSDPLNPSLIHSVDISSMGDGLPADV
                     TVAAGPGAGTYEGNKATFGAVLEAGVPATGTLEIVDDGVAGGTVNDGCEEFDLLNEAP
                     PGPQSTIAIIDRGFCDFWVKVANAEDAGAEGVIIVNNQAGTPGQLGGTPDDPNDTDIL
                     AVHVSDVDGAAIKTSAGEEATLVWDGVTLSGCGSHTLTGVPDLAEGLLRVYVGGSSGD
                     CPGMDLVEIPLNNPTAATWTGMAVANRSCHDITVYLIDKNRAACSGSWEGEHGYTYFS
                     MDAADGGSLADPVLLYERNMEGTFTIGHTSGFSWDGKYLYWSHEPGGGTAAQCETNDP
                     ITNRQMWIFRAERGNELGRWTIPSQSAQENCASVHIMQSIPTNNGSYVLTTGTYQAGT
                     YILDVTDPEFAHAIGWSDPPARVPTTLLAGAWATYWYNGYLYESDILYGLHIHRSTSP
                     QAQSPYVLPFSNPQTMMALPALPKCRGQDATLVGTPGGDTLTGTGGPDVIVGLAGNDL
                     IRGRGGNDLICARAGADTVRGNAGNDEILGQAGRDTLFGNKGNDLLNGGGARDSCFGG
                     PGRDQARRCERTRSIP"
     gene            4206..4514
                     /gene="rpmE"
                     /locus_tag="VF097_11115"
     CDS             4206..4514
                     /gene="rpmE"
                     /locus_tag="VF097_11115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX6263384.1"
                     /translation="MKADIHPEYVEATVRCSCGNTFQTRATKPDIHVEICSNCHPFYT
                     GKQKLVDSGGRVERFRRRYEGRPAPSALRRRPQEEAEAQADASVEAQAPAEAAEPTAE
                     "
     gene            4516..4890
                     /locus_tag="VF097_11120"
     CDS             4516..4890
                     /locus_tag="VF097_11120"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX6263385.1"
                     /translation="MTPEALRSWLDAYGRAWETRDPDAAIELFSPDATYRETPFDEIM
                     EGREAIRQYWQQVPDYQRDIEFGYEIVAAEPVVVRWWSAYTKISNGDRVKLDGVFLLE
                     FDEDDLCTSLREWWHADETPAF"
     gene            4917..5843
                     /locus_tag="VF097_11125"
     CDS             4917..5843
                     /locus_tag="VF097_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019878450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEX6263386.1"
                     /translation="MSEKQHFYGGQAVLEGVMMRGAEVWAIAVRRPDGGIHRESHEID
                     SVAKRYPILRRPGLRGVIALGQALSIGVKALTISANHSVEEEEKLTPRQMAVSMIIAF
                     VFFIGLFIVGPAVLFGFARDQISSGILLNIGEGIFRVALFLGYLLLIGRMKDIRRVFE
                     YHGAEHKTIAAYEHDEPLEPEAVDKYSTLHVRCGTNFLLIVMVLTIFVFTLFGTPGIW
                     WRIGSRILAIPLIAGLAFEALRIGARYPRSRVVRALMAPGLWLQKITTKPPSEDQIEI
                     AIASFEEVLRQEREVGEAPAAEGPVPIPEPED"
     gene            5843..6910
                     /gene="prfA"
                     /locus_tag="VF097_11130"
     CDS             5843..6910
                     /gene="prfA"
                     /locus_tag="VF097_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX6263387.1"
                     /translation="MRERLEEIERQYEEVGRELSEPTVASDPGKLRDLGKRHAELGEI
                     VRAYREWLRVGQDAEEARALAREETDAEAAATWREEATRASARAKELEAEIEVLLLPK
                     DPNDERNVILEIRAGTGGDEASLFARDLFEMYRRYAEGRRWKTDVLSSSPSAAGGFKE
                     VVSEIRGQGAYSRLKHESGVHRVQRVPETESSGRIHTSTATVAVLPEAEDVDVEIRDE
                     DLHIDVYRSSGPGGQSVNTTDSAVRITHTPTGIVVSCQEERSQRQNKEKAMRYLRARL
                     LKMAQEEQAAEEAAARKAQVGTGERSEKIRTYNFPQSRVTDHRIGLTSHRLEGILAGE
                     IDEFVDALLAEERARQLQSTG"
     gene            7014..7850
                     /gene="prmC"
                     /locus_tag="VF097_11135"
     CDS             7014..7850
                     /gene="prmC"
                     /locus_tag="VF097_11135"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX6263388.1"
                     /translation="MDASRLVAETAKFLADHDVETPEAEAEILLAHLLRTNRAGLYAR
                     SEDLTDEERDLLARMLLVRASGVPLQHLTGDQPFFGLRLRVRPGVFIPRPETEVLVEQ
                     ALQCLRTATRPIVVDVGMGTGAVALAIKKERRDARVLATDASEEAVALAGENARELGL
                     GIEVLRGDLLSPLPGDLRGEVDLIVSNPPYVTEEEYAQLPDVVRADPREALVGGTGLH
                     ARLAREAPAWLQPGGWLAMEIGAGQGDEVAGLLSERYERVEVVPDLAGLDRVARGRLP
                     EA"
     gene            7847..8455
                     /locus_tag="VF097_11140"
     CDS             7847..8455
                     /locus_tag="VF097_11140"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016441734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX6263389.1"
                     /translation="MSEVVGPDEAGIEAAIRALDAGEAIVLPTDTVYGLGARPEQAAR
                     LFEVKGRPRDLTLPVLVARVEDAREVAVLDERAETLAASFWPGALTMVLGRATRSAGW
                     DLGAHETTVGVRVPAERVARTLLERTGPLAVTSANRSGEPTPDTCEGVREMLGEDVAV
                     YLCAGTAPGGTPSTVVDLAGDPRVIRAGAIPIDDLLAALEAG"
     gene            8493..>9144
                     /locus_tag="VF097_11145"
     CDS             8493..>9144
                     /locus_tag="VF097_11145"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6263390.1"
                     /translation="MTAMPDTFDVGIVGAGIHGAAAAYHLSAKGLRTVIFERDAPAGG
                     PTGESSAICRAYYTNPFLAGCARDSIEMLRSFEAITGQDAEFRRTGLLFLHPPEDEER
                     VRQSAGQLSDLGIPVEVLSREAVGERFPALDLAGIGIAAWEPEAGYADPVAATTGLLG
                     AAAKSGAELRAGEAVASLEGADGGGAIVGTAAGEPVRCARLLIAAGPWTRSLAAQLG"
BASE COUNT         1492 a         3012 c         3300 g         1340 t
ORIGIN      
        1 ggtccagctc gcgatccagc gcaagaagaa cagcgggaag tgccagtggt ggaacgggaa
       61 gcggttccgc ggcgggaagt gcaagcgcgt ccctccgaag ttctggaaga aggcgaagta
      121 cgatcgcttc ctcgactggt atctgtatcg agtgccctcc ttcaaaccga ccgtggggac
      181 gaatatccgc ttctaccgcg ccttcgcccg tgggttcgat cacgcgggca acgtggagac
      241 ccgcttccag aagaagcgta acaagaacac cttctggatc aagcgcaggt agcggctgag
      301 gccctcaagc atttccggga agttgtggta gcgtccgctg gtcggtgacg gatctcggtc
      361 cgtctggaat gaggagggcg tctcttgagc tcacgtaggc tcgtgctggt actggctgcc
      421 ctgacgatta ttgtcgcttc attcccagcg acgggcgtcg caagcagcgc ttctggagag
      481 accgcctccg cgggcacgag cctcgcctgt gcggggaacc tctccttcca cagcgagacc
      541 cacctcatcg gtatggaggg tacggccgcg gcgtcggact tctacgcagt tccagaggac
      601 gggtctatca acgcgacgct cacggccacc cccgctgaga cgtgcacgga ggacccgaac
      661 gacttcgctc ggccgggcgc ggccgactac cagaccgtgc cggggagcgc tgtggccccc
      721 gacgactacc aaggggtctc cggtaccagc gctccgatct gcgttgaggg cacgcccgtg
      781 ccaccgcacg atccgtgtcc ggccggcacg gcccccacac gcaccgttgg gctcaacctg
      841 gtgaatgacg aaggcgtcga ggacgccgtg gagtccttca acttcgtgct ctccggggcg
      901 acggggtttc cccctttgag ttcgaaccgt cccaccgagg cagcggtgca catcatcgac
      961 aacgacggcc ccgccagggt gtcgctggag ccgacgctcg acggagggga cacgatgcag
     1021 tacgagcccg gggagcaggc cagtcacttc cacatccccg tgttctgggc gggagagacc
     1081 ctgccgagcg ggactgttcc atacaccata ggtcccgccg atgggttccc cacgccgacg
     1141 ctcgacgtgg actacgaggt caactccccg gcccagctac cggcgagcga ttttggcgga
     1201 ggccgggtgg ccttcatcaa gctccggatc ctcaacgaca acaaagaaga actcgacgaa
     1261 gccatcacga tcacgctaac cggggccaat gcggtggagg gtcgcgacga gaccactgtc
     1321 ctcatccgcg atgcgggggt ggacaacctc gcgccgagga cgaacttcca ccacccgaag
     1381 cacaagaagc ggtacccgag aaacgcgtat cagctgcggg agatgcacat cttccacagc
     1441 gatcccggcg gctccggcat ccggcgggtc cagatcgcga tccagcggag gctcacgaac
     1501 ggtcgatgcc aatggtggaa cgggaagcgg ttcgccggag gcagctgcaa gggcgtcccg
     1561 gcgaagttct ggaagaagac gaagtacgag cgagccctcg actggttcct ctatcgcgtg
     1621 cccgccttca agccgacgca gggcacgaac ctccgcttct atcgtgcctt cgcccgcgga
     1681 ttcgacatgg ccggcaacgt cgagagacgc ttccagaagg gccgcaacaa gaacacgttc
     1741 tgggttaagc gctcgtaaga ggcggcctcc gcccctcgca actggatttg ttctttcctc
     1801 tcatccccag gtatcatccg cgcacgcttc agggggtgtg ccggggaaac catggcaggg
     1861 cgagagggag gtcggtgtac cgttcgtccc tgttccctct gccgtgcacc tagccgtccc
     1921 tgtgaagcgg gccttggggg ccgcagccct ccccatcgac gttcccggat ggctgcccga
     1981 cgtttcgaga aggaggcgag cacatgagta ggaggacacg gtggttggtc ttgccgatca
     2041 ccttggtggc cgtcctggcg ctggggatac ccccaagctc ggcggaccac gcaacccgac
     2101 aggacgtcaa gaacctggtg ccggtggcgg acgtcgagaa cagcgtaccg tttgccatca
     2161 actccgacat ggcgttctgg ggtgacctcg cgtaccaggg caacttcgag gggttccaga
     2221 tccacgacat cagcgatccg gagaacccgg tcaacctcgc ggacgtcgag aactgtccgg
     2281 acggccaggg tgacgtcttc gtctgggacg atctgctggt gtactcaagg aactcgccgg
     2341 cgaacaacga cacctgcgac ggggtggctc tgccgaacgg gttcgaggga gttcatcttt
     2401 gggacgtgag cgatcccctc aacccgtcgc tgatccacag cgtggacatc tcgagcatgg
     2461 gtgatggcct gcccgctgac gttaccgtgg ccgccggccc cggggcgggc acatacgaag
     2521 ggaacaaggc aaccttcggt gccgtgcttg aggcgggtgt cccggcgacc ggcaccctcg
     2581 agatcgtcga tgacggcgtg gcgggcggca ccgtcaatga cggctgcgag gagttcgacc
     2641 tgctgaacga ggctccgccg ggtccgcagt ccacgatcgc gatcatcgac cgtgggttct
     2701 gcgacttctg ggtgaaggtg gccaacgccg aggatgctgg cgccgagggc gtcatcatcg
     2761 tgaacaacca ggccgggacc cccggtcagc tgggtggcac gcccgacgat cccaacgaca
     2821 ctgacatcct cgcggtccac gtctcggacg tcgatggcgc cgccatcaag acgagcgcgg
     2881 gcgaggaggc cacgctcgtg tgggacggcg tcacgctcag cggctgtggt tcgcacacgt
     2941 tgactggcgt tccggacctc gctgagggtc tcctgcgcgt gtacgtgggt ggatcgagcg
     3001 gggactgccc ggggatggac ctcgtggaga tcccgctcaa caacccgacc gcggccacgt
     3061 ggacgggaat ggcggtcgcg aaccggtcat gccacgacat caccgtctac ctgatcgaca
     3121 agaacagggc ggcgtgctcg ggcagctggg agggcgagca cgggtacacg tacttcagca
     3181 tggacgccgc cgacggtggg tcgctcgcgg acccggtgct cctctacgag cggaacatgg
     3241 agggcacctt cacgatcggt cacaccagcg gcttctcgtg ggacgggaag tacctgtact
     3301 ggagccacga gccgggcggc gggacggcgg cgcagtgtga gaccaacgac ccgatcacga
     3361 accgtcagat gtggatcttc cgtgctgagc gggggaacga gctgggcagg tggacgatcc
     3421 cctcgcagtc ggcgcaggag aactgcgcgt cggtccacat catgcagagc atcccgacga
     3481 acaacgggag ctacgtgctg acgacgggga cctaccaggc cgggacgtac atcctggacg
     3541 tcaccgaccc ggagttcgct cacgcgatcg ggtggtccga cccgcccgcg cgggtgccga
     3601 cgaccctcct cgcgggtgct tgggccacgt actggtacaa cggctacctg tacgagtccg
     3661 acatcctgta cggtctgcac atccatcggt cgacctcgcc tcaggcgcag tcgccgtacg
     3721 tgctgccgtt ctccaacccg cagacgatga tggcactgcc ggctctgccg aagtgtcgag
     3781 gacaggacgc cacgctcgtg ggcacgcctg gcggcgacac cctgaccggc accggtggtc
     3841 ctgacgtcat cgtgggcctg gcagggaacg atctgatccg cggtcgcggt ggcaacgacc
     3901 tgatctgcgc ccgtgccggc gcggacacgg tccgaggcaa cgccgggaac gacgagatcc
     3961 tgggtcaggc cggccgtgac acgctgttcg gcaacaaggg caacgacctg ctgaacggcg
     4021 gcggtgcccg ggactcctgc ttcggaggcc ccgggcggga ccaagctcgc cgttgcgagc
     4081 ggacgcggag tatcccgtag ggtccgacgt agcagacgca cgatcgaggc ggcccccggg
     4141 cgagcgccgg ggggccgccc cacttcccgg tgacggcgtt gacaccctcg cgtatgcttg
     4201 ggtccgtgaa ggctgacatc caccccgagt acgtcgaggc gaccgtccgg tgctcctgcg
     4261 ggaatacctt ccagacgcgc gccacgaagc ccgacatcca cgtcgagatc tgctcgaact
     4321 gccacccctt ctacacgggg aagcagaagc tggtggactc gggcggccgg gtcgagcgct
     4381 tccgccgccg ctatgagggg cgtccggccc ccagcgctct acggcgccgg ccacaggagg
     4441 aggcggaggc tcaggcggac gcttccgtgg aggcccaggc tcccgccgag gcggccgagc
     4501 cgaccgcgga gtagcgtgac ccccgaggcc ctcaggtcct ggctggacgc ctacgggcgg
     4561 gcctgggaga ctcgagaccc cgatgccgcg atcgagctgt tctcgcccga cgcgacctac
     4621 cgcgagacgc ccttcgacga gatcatggag gggcgcgagg cgatccgtca gtactggcag
     4681 caggtccccg actatcagcg cgacatcgag ttcggctacg agatcgtggc cgccgagccc
     4741 gtggtcgtcc gctggtggtc cgcctacacg aagatcagca acggcgaccg cgtgaagctc
     4801 gatggcgtct tcctcctgga gttcgacgag gacgacctgt gcacctcgct ccgggagtgg
     4861 tggcacgccg acgagacccc ggccttctga gatcagggcg cccgtacaat gggtcggtga
     4921 gcgagaagca gcacttctac ggcggtcagg cggtcctcga gggcgtgatg atgcgggggg
     4981 ccgaggtctg ggcgatcgcc gttcgcaggc ccgacggggg catccaccgc gaatcgcacg
     5041 agatcgactc cgtggcgaag cggtatccga tcctgcgcag gcccggcctg cgcggggtga
     5101 tcgcgctggg ccaagccctc tcgatcgggg tgaaggccct cacgatctcc gccaatcact
     5161 cggtcgagga ggaggagaag ctcaccccgc gccagatggc ggtctcgatg atcatcgcgt
     5221 tcgtcttctt catcggactg ttcatcgtgg ggccggcggt gctgttcggg ttcgcccggg
     5281 accagatctc gagcggcatc cttctgaaca tcggggaggg gatcttccgg gtcgcgctct
     5341 tcctcgggta cctgctgctg atcgggcgga tgaaggacat ccggagggtg ttcgagtatc
     5401 acggggccga gcacaagacg atcgcggcct acgagcacga cgagccgctg gagcccgagg
     5461 ccgtggacaa gtactcgacc ctccacgtgc ggtgcggcac caacttcctg ctgatcgtga
     5521 tggtgctcac gatcttcgtc ttcaccctgt tcgggacgcc gggcatctgg tggaggatcg
     5581 gctcgcggat cctggccatc ccgctgatcg cggggctggc gttcgaggcc ctgcggatcg
     5641 gggcgcggta cccgaggtcg cgggtggtgc gggcgctgat ggctccgggc ctgtggctgc
     5701 agaagatcac gacgaagcct ccctccgagg accagatcga gatcgcgatc gcgtccttcg
     5761 aggaggtcct gcggcaggag cgggaggtgg gagaggctcc ggccgccgag ggcccggtcc
     5821 cgatccccga gcccgaggac tgatgcgcga gcgcctcgag gagatcgagc ggcagtacga
     5881 ggaggtcggt cgggagctct ccgagccgac cgtggcctcc gacccgggca agctccgcga
     5941 cctggggaag cggcacgccg agctggggga gatcgtgcgg gcctaccgcg agtggctgcg
     6001 ggtcgggcag gacgccgagg aggcgcgtgc gctcgcccgc gaggagacgg acgcggaggc
     6061 cgctgcgacc tggcgcgagg aggccacccg ggcgagcgcg cgcgccaagg agctcgaggc
     6121 cgagatcgag gtcctgctgc tgccgaagga ccccaacgac gagcgcaacg tgatcctcga
     6181 gatccgcgca ggcacggggg gcgacgaggc ctcgctcttc gcccgcgacc tcttcgagat
     6241 gtaccggcga tacgcggagg gacggcgctg gaagacggac gtcctgtcct cgagcccctc
     6301 ggccgccggg ggtttcaagg aggtcgtcag cgagatccgg gggcagggcg cctactcgcg
     6361 gctgaagcac gagtcagggg tgcaccgggt ccagcgggtc cccgagacgg agtcgagcgg
     6421 ccgcatccat acgtcgacgg ccaccgttgc ggtcctgccc gaggccgagg acgtggacgt
     6481 cgagatccgg gacgaggacc tccacatcga cgtgtaccgg tcctcgggac cgggcgggca
     6541 gtcggtcaac accaccgact cggcggtccg gatcacgcac acgccgacgg ggatcgtcgt
     6601 gtcgtgccag gaagagcggt ctcagcgcca gaacaaggag aaggcgatgc gctacctgcg
     6661 ggcgcgcctc ttgaagatgg cgcaggagga gcaggcggcc gaggaggccg cggcgcgcaa
     6721 ggcgcaggtg gggacggggg agcgctcgga gaagatccgg acctacaact tcccccagag
     6781 ccgcgtgacc gaccaccgga tcgggctgac ctcgcaccgg ctcgagggga tcctggcggg
     6841 cgagatcgac gagttcgtgg atgccctgct ggccgaggag cgcgcccgcc agctccagtc
     6901 gaccggctga catacgccca ctcctcggtg acgccggtcc tcgggctgag gcgtcaggct
     6961 ccctcgcttc ccgccctcaa ggggacccgt gagtgcgtcg atagactgct ttcatggatg
     7021 cgtcccggct cgtcgccgag accgccaagt tcctggccga ccacgacgtg gagacgccgg
     7081 aggcggaggc ggagatcctc ctcgcgcacc tcctgaggac gaaccgtgcc gggctctacg
     7141 ccaggagtga ggacctgacg gacgaggagc gcgatctcct ggcccgcatg ctgctcgtgc
     7201 gcgcgtcggg ggtcccgctg cagcacctga ccggcgatca gcccttcttc gggctcaggc
     7261 tccgggtgcg gccgggcgtg ttcatcccca ggcccgagac cgaggtactc gtggagcagg
     7321 ccctccagtg cctgaggacg gccacccgcc cgatcgtcgt ggacgtcggg atgggcacgg
     7381 gcgcggtggc cctggcgatc aagaaggagc ggcgggacgc cagggtgctc gccaccgacg
     7441 cgtcggagga ggcggtggcc ctggcggggg agaacgcgag ggagctcggc ctcgggatcg
     7501 aggtgctccg gggcgatctg ctgtcgccgc tgccgggcga tctccggggg gaggtggacc
     7561 tcatcgtgag caaccccccc tacgtcaccg aggaggagta cgcgcagctg ccggacgtgg
     7621 tccgcgcgga tcccagggag gcgctggtgg ggggcacggg gctccacgcc aggctggccc
     7681 gggaggcgcc cgcctggctc cagcctgggg gctggctggc gatggagatc ggggccggcc
     7741 agggcgacga ggtggccggc ctgctgtcgg agcggtacga gcgcgtcgag gtcgtccccg
     7801 acttggccgg gctggaccgg gtggcgcggg ggcgtttgcc ggaggcatga gcgaggtcgt
     7861 cgggcccgac gaagcgggga tcgaggccgc gatccgggcg ctggacgcgg gcgaggcgat
     7921 cgtgctcccc accgacacgg tgtacgggct gggggcgagg cccgagcagg cggcgcggct
     7981 cttcgaggtc aaggggcggc cccgggacct gacgctcccg gtgctggtgg cgagggtcga
     8041 ggacgcgagg gaggtggccg tgctcgacga gcgggccgag acgctggcgg cctcgttctg
     8101 gccgggggcg ctgacgatgg tcctggggcg cgcgacgcgc tcggcgggct gggacctcgg
     8161 cgcccacgag acgaccgtcg gcgtccgagt ccccgccgag cgcgtcgcgc gcaccctcct
     8221 cgagcgcacc ggccccctgg ccgtgacgag cgccaaccgc tccggcgagc cgacgcccga
     8281 cacgtgcgag ggggtccgcg aaatgctcgg ggaggacgtc gccgtctacc tctgcgcggg
     8341 gaccgcgccc gggggcacgc cctccacggt ggtcgacctg gccggcgatc cacgcgtgat
     8401 ccgcgccggg gccatcccga tcgacgatct cctcgccgcg ctggaagccg gctgagcgcc
     8461 gggggttgcc ccgcgtcgcc ggagcctcca cgatgacggc catgcccgac acgttcgacg
     8521 ttgggatcgt cggggccggc atccacggcg ccgccgccgc ataccatctc tcggcgaagg
     8581 gcctccggac cgtcatcttc gagcgcgatg ccccggccgg cggccccacc ggcgagtcca
     8641 gcgcgatctg tcgcgcctac tacacgaacc cgttcctggc cggctgtgcc agggacagca
     8701 tcgagatgct ccgcagtttc gaggcgatca ccgggcaaga cgcggagttc cggaggaccg
     8761 gcctcctctt cctccacccg cccgaggacg aggagcgggt acggcagagc gccgggcagc
     8821 tgtcggatct cgggatcccc gtcgaggtgc tgagccggga ggcggtgggg gagcggttcc
     8881 ccgcgctcga cctggcgggg atcgggatcg ccgcctggga gcccgaggcc ggatacgccg
     8941 atcccgtggc cgccacgacg ggcctcctcg gggccgcggc gaagagcggc gcagagctgc
     9001 gagcggggga ggcggtggcc tctctcgagg gagcggacgg gggcggcgcg atcgtcggga
     9061 cggccgcggg ggaaccggtc cggtgcgctc gcctcctgat cgcggccggc ccctggaccc
     9121 gctcgctcgc cgcccagctc ggcg
//
LOCUS       DASUTZ010000261        16814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1915751, whole genome shotgun sequence.
ACCESSION   DASUTZ010000261 DASUTZ010000000
VERSION     DASUTZ010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 16814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16814
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1915751"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..367
                     /locus_tag="VF097_11150"
     CDS             176..367
                     /locus_tag="VF097_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263391.1"
                     /translation="MELPPGRREEIHRHEERGCETPLHGIAVALWTQFVESQARLEEG
                     RRRAVRAREEQEAARGRYL"
     gene            complement(497..946)
                     /locus_tag="VF097_11155"
     CDS             complement(497..946)
                     /locus_tag="VF097_11155"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6263392.1"
                     /translation="MKTGAEISPDEARGIAVFEGLSETDLHRLLERSRAVSFSPGESI
                     FTRGETGCSFYVITSGKARVDVGGRYHDLTKGDLVGEMAALTSQPRMATVRVMHEVDA
                     IEIDCGSDTDAFLMEHPALAVRIIKILVGRLREVEDRLDAWMGIHRA"
     gene            1111..1746
                     /locus_tag="VF097_11160"
     CDS             1111..1746
                     /locus_tag="VF097_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014628767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX6263393.1"
                     /translation="MRTRDALRRRALRLEYATLAWNVAEAFWTIALGIAAASLALIGF
                     GVDSVIEIFASAVVVWHIRPGHEHDHPERTARALRLVAAAFVALALVLGVAAGRDLVV
                     GRRAEESPLGIAVLAITAVVMFGLAAAKRRTADELDSAPLRSEAAMTFLDGILATATL
                     TGLALNAIAGWWWADPAAAILVAVAALNEARENLGEAGEIVREGIPQEPKR"
     gene            1772..2992
                     /locus_tag="VF097_11165"
     CDS             1772..2992
                     /locus_tag="VF097_11165"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase regulatory domain-containing
                     protein"
                     /protein_id="HEX6263394.1"
                     /translation="MRLSFQQLADRAGVDEDFVRRLHALGALHTSQEPFGERDLHLVA
                     LLHRWEEAGIGAETVLRAMEQGDLSLSFLATPGFELTGRLDRTYRQYAEEARVPLELI
                     LSLHEAIGFARPDPDDRIREDDEVLVEMAAAFLESGASETAVRRMFRIYGDNLGRLAA
                     AIGDVYRAEIEQRFREGGATDTDVMERGARLGRRVGSLMHRALVAIFERHHQHVWTGY
                     SIGWAEASLERAGLRQRVERPPAICFVDLTGYTSFTEERGDEAAARLAASLAALVDEI
                     SLRRGGRAVRWLGDGGMFLFDDPKAAVLAGLDMAERAPAEGLPPTHVGIQAGPVIFQD
                     GDVYGRTVNIASRIAARAGPGEVLVGAEVAELARGPEVRFEPIGPAELKGLAEPMALY
                     RALRDREGEPRPSD"
     gene            3010..3288
                     /locus_tag="VF097_11170"
     CDS             3010..3288
                     /locus_tag="VF097_11170"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEX6263395.1"
                     /translation="MKRTQIYLDEAQDERLTKRARASGTTKSDLIRVAVDAYLAGPKD
                     RTTHLMAFRTAVRAAAGSIPRLPTGRRYVDEARRADQERERDLEARRR"
     gene            3285..3662
                     /locus_tag="VF097_11175"
     CDS             3285..3662
                     /locus_tag="VF097_11175"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX6263396.1"
                     /translation="MSRVLDTTVLIDVLRGHEPAVMFVLSLEDVPVCSEVTRVEIIRG
                     LRSEERRSAERLFRQLRWVPVDETIARAAGELGRSLRRSHTGIGIADLIISATVEEVG
                     LPLATTNVRHFPMLEGLRAPYRG"
     gene            3681..3974
                     /locus_tag="VF097_11180"
     CDS             3681..3974
                     /locus_tag="VF097_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263397.1"
                     /translation="MTHVGLLSAAWATAQAEERLTKSSTSRSPRILGVMGGPLIVVSA
                     FLSWIAPATSAEGTSMPVSLFWSTEFSTMDSDPSFFASAGFIVILIGAVTVTG"
     gene            complement(4057..4130)
                     /locus_tag="VF097_11185"
     tRNA            complement(4057..4130)
                     /locus_tag="VF097_11185"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4094..4096),aa:Lys,seq:ttt)
     gene            complement(4139..5764)
                     /locus_tag="VF097_11190"
     CDS             complement(4139..5764)
                     /locus_tag="VF097_11190"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX6263398.1"
                     /translation="MRIALGQLNTTVGDLEGNVERLAAGAVEAAGQGADLVVFPELAI
                     TGYPPEDLLLRPAFVEDNLEALQELARRSGETTAVLVGFVDRTDLGMHNAAALLRDGR
                     VEARYHKHKLPNYGVFDEARYFVPGGEGCALRVADAELGISICEDAWKPGPPFDGYAE
                     AVQIIPNLNASPYHRGKIGQRIEICADRARETGAWIVYVNAVGGQDELVFDGGSMVVA
                     PNGERAWHADMFEEDMLVVDLPEATERKPRLPDPEREPWPEGPDEVYRALVLGLGDYV
                     RKNGFTDVVLGLSGGIDSSLTAVVAVDALGPDHVWGLAMPSRFSSDHSLEDAEELANR
                     LRIRLDRVPIDDTFQAYLDALAPIFEGTEPGVAEENVQARIRGNLLMAASNKFGHLVL
                     ATGNKSEMAVGYSTLYGDMAGGFAPLKDVLKTLVYDLARWRNEQTDPPIPDRVLTKPP
                     SAELRPDQQDTDSLPPYEVLDPIVEAYVEEDRAPEEIVADGADAPLVDRVVRMIDGSE
                     YKRRQAAPGVKITPKAFGRDRRLPITNRYRRAP"
     gene            complement(5784..7112)
                     /locus_tag="VF097_11195"
     CDS             complement(5784..7112)
                     /locus_tag="VF097_11195"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX6263399.1"
                     /translation="MTLTKEAEFVLRTVEERGIRFIHLWFTDVLGFLKSFAITPQELE
                     TAFTEGMGFDGSSIEGYSRIQESDMVAIPDPTTFQVIPWRSEEGVARMYCDVVNPDGT
                     PFEGDPRWVLTRQLQRAADLGFTFYIGPELEYFYFQDDGGTEFLDRGGYFDLTPRDVA
                     TDYRKRTVGYLEAMGIPVEYVHHEVAPSQHEIDLRYTDALTMADNVMTYRLTVKETAQ
                     EFGVHATFMPKPVAGVNGSGMHTHQSLFEGDRNAFFDGTDEYHLSKTAKGYIAGILRH
                     TPEITLVLNQWVNSYKRLVPGYEAPVYICWARRNRSALVRVPMYKPGKESATRIELRS
                     PDPACNPYLAFAAMLAAGLKGIEEEYEIPPEASNNIYEMSEEERREAGIEHLPEDLHD
                     AIRAAEDSELLRETLGDHVHEFLIRNKREEWDTYKRYVTPYELERYLPVL"
     gene            7196..8506
                     /locus_tag="VF097_11200"
     CDS             7196..8506
                     /locus_tag="VF097_11200"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX6263400.1"
                     /translation="MENEQVLNEVEERGIRFVRLWFTDVLGFLKSFSIPIEELEKAFR
                     EGIGFDGSAIEGFARVEEADMLARPDAATFRVLPVPREEPAARMLCDITYPDGAPFEG
                     DPRHVLRRNLSRLDGRSFHAEPEVEFFLSGSEERFQPLDHGSYFDLTASDAAEEFRRR
                     AVPVLETLGIEIRMLHHEDAPGQQEIDLAPGDALRVADDLITLRLLVKEVAQELGLHA
                     SFMPKPAPGIQGSGMHVHYRLLEGERNAFFDPTDEARLSKEGRSFVAGLLEHAPAMTA
                     VTNQWVNSYKRLVPGFEAPIHVSWARRNRSALVRVPADRPDDEASCRVEYRGADPACN
                     PYLAFAVVLAAGLDGIERGLEAPPEVHEDLASLSDEELRALGVRSLPDNLHDAIAEME
                     RSDLLAEALGERLFEWLIRNKRDEWEAYRTYVTPFEVERYFPLL"
     gene            8506..11139
                     /locus_tag="VF097_11205"
     CDS             8506..11139
                     /locus_tag="VF097_11205"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HEX6263401.1"
                     /translation="MRSPEALGRALAAAPDPEMARVALSRVGEDPRVRVALEEPGAAE
                     MAVRLLGFSGPAADFLVAYPEEAGLFSELGPRSREALLGEARADLERLGGEAGLRRFR
                     RRALLRVAARDLGGAVLEEVVAEVTSVAEVCLALAVEAAGAGGLAVIGMGKLGGEELN
                     YSSDVDVIFVHEEAAPEEAARAASRLIALLSEPTRDGVALRVDAALRPEGRAGPLSRS
                     LASTVEYYVRHAATWERQALLKARPVAGDLELGRRLLAELDPVIYPEHLDPEAIDDIR
                     AMKARLEDHVRARGKEESEVKRGRGGIRDVEFAVQLLQLVHGRRDARLRSRGTLRALA
                     DLAEQGYVAPGDAEALAGSYRFLRTVEHRLQIARDLQTHELPEDRPSRRRLARSLGIA
                     SPAELGAAYERHAEVVRGLHERLFYRPLLEAFAGPRLPRPAEERAQTEELLTALGFRE
                     PPAAYERLDRLMDAASRRGRVLEGVFPVVAPALSAAPDPDKALVRFDRVADGLDDEGA
                     DLLAADPTVARRLALLVGASGWAADFLAAQPEAAVLLEGRDRPDARPRERLVAAAGSY
                     VAGELRVPASGRTLARIADDVVAEALRAAQPEVPIAVIALGKLGGEELNFGSDLDVVF
                     VYDGEGEEPFREAVRAAEATLEGIREAGFEPDPDLRPEGKNGPLARSLASFLEYWQRW
                     GQTWEFQSLLKARAVAGEEALARRWLSNAEDLAYPEALPLDREAEIRRMRVRMEKERV
                     RPPEAARFHFKLGHGGLADVTFAVELAQMRHGRLHPPVRRRHTLEALEALASEGLIED
                     GVALALGEAYVFLNEVKNAMELDARRPADALPPTPEAQVALARLLGYEEHPRQAFLEK
                     YRRVTRRARRAMERVFYGEEP"
     gene            11136..11657
                     /locus_tag="VF097_11210"
     CDS             11136..11657
                     /locus_tag="VF097_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263402.1"
                     /translation="MRTVALILALTAVACAEDGAGGLGAAPSPGFEVVERGTEPAGPM
                     IEPSVVLVRGDLQAEAEAANAPAEGAAELIRSWDELDERALLVVYGGTVPDAGTSVRV
                     DDVALEQDARHLIVAATLESEEPALQVVSIAWAAVSLDADLVARADTCALVLDGREVP
                     DAACSVAIPYASK"
     gene            complement(11642..13036)
                     /gene="glnA"
                     /locus_tag="VF097_11215"
     CDS             complement(11642..13036)
                     /gene="glnA"
                     /locus_tag="VF097_11215"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX6263403.1"
                     /translation="MAKDAGAEVVDLRFCDLPGLMQHFSVPVSELTEESFEDGFGFDG
                     SSIRGFQEIQESDMLLLPDAGAAYVDPFRPRPTLVLACFVKDPVTGEMYTRDPRGIAR
                     KAELYLQQTGIAETSYWGPECEFYIFDGIRFDQNQHEGYYHIDAMEGVWNSGRDEEGG
                     NKGYKPRYKEGYFPVPPMDHYQDLRTEMMLNLEKVGIEVEVHHHEVGTAGQAEIDIRY
                     DTLLRTADKVMTYKYVVKNTALAAGKTVTFMPKPIFEDNGSGMHTHQSLWSGEENLFW
                     DEMGYAGLSDSARWYIGGLLQHAPALLAFAAPTTNSYRRLVPGYEAPINLVYSQRNRS
                     ACIRIPVYYKTPVAKRIEFRSPDPTANPYLAFSAMLMAGIDGIKNKTEPPDPIDKDLY
                     DLPPEEAADVRQVPGSLDEVIAALEVDHAFLLEGGVFTQDVIDTWIDYKRRNEIDELR
                     LRPHPYEFYRYFDA"
     gene            13194..13571
                     /locus_tag="VF097_11220"
     CDS             13194..13571
                     /locus_tag="VF097_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263404.1"
                     /translation="MGREMRFRVLATALAATLAFSITGIGTPAQAATSIVRMKGTRNK
                     FRPVHTYIAKGDKVSWRNMSRRVHDVTAYGGGWTFSKVLSPGQRTARRFRKRRTYRYR
                     CVRHSAIVNNRCSGMCGFVHVVA"
     gene            complement(13575..15554)
                     /locus_tag="VF097_11225"
     CDS             complement(13575..15554)
                     /locus_tag="VF097_11225"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEX6263405.1"
                     /translation="MIPGVGRLLRLRSTLRLAVTLTAAGVAILALAGPASGHALLLFA
                     DPADGANLGEAPSTITLTFTEPLEPSLSQIVVEDEGGQEVHQGESRLDPSEPTILSVD
                     LGQLEQGGVYTVTWQVVSTVDGHPTAGIFAFGIGVDPPERGAEEQEVAAAPTATPFEV
                     TARTLLYIGFALLLGASLVGSVAFPQPPVVLRRLALAGAVLATLAGATVGLAQASAAG
                     ASIADAVGTAIGRSAILRAGLALLAAAAIAVPLGSPTRRLLTGAVLGAAAVLAHVSGG
                     HAGAGEPPWPQVASQWAHVTAAGLWVGGLTSLVIGVRGAPDEVKGAAVRRYSRLAGYA
                     LAVVALSGLFRALDEVGGWSALWSTGYGRFVVAKSGLLLGLAGFGAFNRYRMVPRASR
                     ELRGFRLVSGGELVVALGALTAAAALATLVPPASIPETPRAPAPLQVSATEATGGLRV
                     RLGVSPGTPGPNHFEAVILEGGEGVEDADVAIDFVPLAGSGKESTLDLEAVGEGRYEG
                     EGDGLAISGPWQLTVRVGRDGGTTGVPVRVTTLCETHLVELEVGTVSIPTYILQLEDG
                     TSSQGYLLPSGRRRTEIHFLFLDAGGKELRVPEEPEILAGPVGVEPLVLPSERLLTPG
                     HFAATTRLTPGTWRFDSTATLEDGSRISACFEATF"
     gene            complement(15559..16230)
                     /locus_tag="VF097_11230"
     CDS             complement(15559..16230)
                     /locus_tag="VF097_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6263406.1"
                     /translation="MRRLLILTDRRPGDVLPAAHALGTDVKTDSLDATGLSRVPDLSP
                     DLVVADAVPDPERCFGLLRELRARGGPPSLVVLGRDQLGMLPWQEVADDFVHPETGQD
                     ELRLRVELLRARVGETGEGVLRLGPVAINTLTYRVTAGGRALDLTFKEFELLRFLAQH
                     EARVFTRAQLLREVWGYDFYGGTRTVDVHVRRLRAKLGPEHEHLIETVRSVGYRASFG
                     ERPLD"
     gene            16282..>16814
                     /locus_tag="VF097_11235"
     CDS             16282..>16814
                     /locus_tag="VF097_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006542392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6263407.1"
                     /translation="MIARLLSRRYRSAAPFGATTADGVPLVGVRLGEGPAALVLCHGF
                     MGWHRKASIVSFAERLAERFLVYVFDLRGHGGSAGVCTYGDREYLDVDAVVGLARRER
                     GLPVATLGASMGGIAVIRQAALGEPPDAVMAVSTPARWEGHATAAVRRVTRMTATPSG
                     RRMMRALGFRLTDTWGSP"
BASE COUNT         2309 a         5852 c         6130 g         2523 t
ORIGIN      
        1 ccagcgcgcg ggcctcctcg atcgaggtgt acgtgcgctc gtgggtcatc acctcgtagc
       61 tgaccccccg ctgctccagg tggtcggtga ccctggacat cctcctcacc tccgtcccgg
      121 cccgcccggg ccgtctcgtc cattctcccc cggatcaagc cgctgccggg agccggtgga
      181 gcttccgccc ggccggcgag aggagatcca ccggcatgag gagcggggct gcgagacccc
      241 gctgcacggg atcgcagtcg ccctgtggac gcagttcgtc gagtcgcagg cccgcctgga
      301 ggaggggcgc cgccgagccg tacgggctcg agaggagcag gaggcggcca gggggcgcta
      361 cctgtgacgg caggtcctac cgtcggggcg gcccgctcga cccttctgca gccgccgtgg
      421 gacgtctttc ggcccgagcc gtcgaggaag acgagcccca ggcggggttc gtcggatgga
      481 cggcggttcc cgagccctag gcgcgatgga tgcccatcca ggcgtcgagc cgatcctcca
      541 cctcgcgcag tcgcccgacc aggatcttga tgatccgcac ggccagagcg gggtgctcca
      601 tcaggaacgc gtctgtgtcg gagccgcagt cgatctcgat ggcatcgacc tcgtgcatca
      661 cccgcaccgt cgccatgcgc ggctgcgacg tcagcgcggc catctcccct acgagatccc
      721 ctttggttag atcgtggtac ctgcctccca cgtcgacccg ggccttcccc gaagtgatga
      781 cgtagaagga gcagccggtc tcaccgcggg tgaagatcga ctcaccggga ctgaagctca
      841 ccgcgcgcga ccgctcaagc agcctgtgga ggtctgtctc cgaaaggccc tcgaagacgg
      901 cgatcccccg ggcctcgtcg ggggagatct cggctccggt cttcatggct cctcacgccc
      961 ctctccgtca gcgcgatcca agcatggctc gtcaggaggc gcggagggag ggtcgaacgt
     1021 ccccgaggac gcgggcctct gggcgtccat ccggccggac gcctgctgga tgcgtctctc
     1081 gcacgccgtc ggcgaggcat catgtccggg atgcgtaccc gtgatgccct ccggcgaagg
     1141 gctctgaggc tcgagtacgc caccctggcc tggaacgtcg ccgaggcctt ctggacgatc
     1201 gcgctcggca tcgccgcggc ttccctggct ctcatcgggt tcggggtcga ctccgtgatc
     1261 gagatcttcg cctccgcggt ggtggtgtgg cacatccgcc cgggccacga gcacgatcac
     1321 ccggagcgta cggcgcgggc cctccggctc gtcgcggccg cgttcgtggc tctggccctc
     1381 gttctcgggg tggcggccgg gcgtgatctc gtggtcggga ggcgggccga agagtccccg
     1441 ctcgggatcg cggtcttggc catcacggcc gtcgtcatgt tcggcctggc cgcggcgaag
     1501 cggcgaacgg cagacgagct cgattcggct cccctccgat ccgaggccgc gatgaccttc
     1561 ctggacggga tcctggccac ggccaccctg accggactcg ccctcaacgc gatcgcgggg
     1621 tggtggtggg cggacccggc ggcggcgatc ctggtcgcgg tggccgccct gaacgaggcc
     1681 cgagagaacc tgggcgaggc cggggagatc gtgagggagg gaatcccgca ggagccgaag
     1741 cggtagcgtg tccgaaggga gggatccgag cgtgcgcctg tcgttccagc agctcgccga
     1801 ccgagcgggg gtggacgagg acttcgtccg gcggctccac gcgctagggg cgctccacac
     1861 gtcgcaggag cccttcgggg agcgcgacct gcacctcgtg gccctcctgc accgatggga
     1921 ggaggccggg atcggtgcgg agacggtcct cagggccatg gagcaggggg acctttccct
     1981 gtcattcctg gcgacccccg ggttcgagct cacgggacgc ctggaccgga cctatcggca
     2041 gtacgccgag gaggcccggg tccccctcga actgatcctg agcctgcacg aggcgatcgg
     2101 cttcgcccgg cccgatcccg acgatcggat ccgggaggac gacgaggtcc tggtcgagat
     2161 ggccgcggcc ttcctggaga gcggggcctc ggagacggcg gtccgccgga tgttccggat
     2221 ctatggagat aacctcgggc ggctggccgc ggcgatcggg gacgtgtatc gggccgagat
     2281 cgagcagcga ttccgtgagg gcggcgccac cgacaccgac gtcatggagc gcggagccag
     2341 gctcgggcgg cgggtgggct cgctcatgca ccgagccctg gtggcgatct tcgagcgcca
     2401 tcaccagcac gtgtggacgg ggtactcgat cggatgggcg gaggcctcgc tggagcgggc
     2461 ggggctccgt cagcgagtgg agcgaccgcc ggcgatctgc ttcgtggacc tcaccgggta
     2521 cacgagcttc acggaagagc ggggggacga ggccgccgcc cggctggccg cgagcctcgc
     2581 cgcgctcgtg gacgagatct ccctccgtcg cgggggtcgg gcggtccgct ggctggggga
     2641 cggtggcatg ttcctcttcg acgatccgaa ggcggcggtc ctcgcgggcc tggacatggc
     2701 ggagagggcc cccgccgagg gcctcccccc gacccacgtg ggcatccagg cgggacccgt
     2761 gatcttccag gacggtgacg tctacggccg gaccgtcaac atcgcctccc ggatcgcggc
     2821 gcgcgcgggg ccgggcgagg tcctcgtcgg tgcggaggtc gcggagctcg ctcgcggccc
     2881 ggaggtgcgg ttcgagccga tcggtccggc cgagctgaag ggcctcgccg agcccatggc
     2941 gctctatcga gccctgcgag accgggaagg ggaaccgcgc ccctcggact gatgtatact
     3001 acgtacatcg tgaagagaac gcagatctat ctcgacgagg cccaggacga gcgcctgacc
     3061 aagcgcgcac gggcgtctgg gaccacgaag tccgacctga tccgcgtagc ggtcgatgcc
     3121 tacttggccg gaccgaagga ccggaccacg cacctcatgg cgttccggac cgccgtgcgg
     3181 gcggcggccg gatccatccc gcgtctgccg accgggcgtc ggtacgtcga tgaggcccgc
     3241 cgggccgacc aggagagaga gcgcgacctc gaggcccgcc gccggtgagc cgcgtcctcg
     3301 atacgaccgt cttgatcgac gtcctgcggg gccacgagcc tgcggtgatg ttcgtgctct
     3361 ctctcgagga cgtccccgtg tgctcggagg tgacgcgggt ggagatcatc cgggggctgc
     3421 ggagcgagga acgacggtcg gccgagcggc tgttccggca gctgcgctgg gttcctgtgg
     3481 acgagaccat cgctcgagcg gccggggagc tcggccggtc ccttcggcgg agccacaccg
     3541 ggatcggcat cgcggacctc atcatctcgg cgacggtcga ggaggtcggc ctccccctcg
     3601 cgaccacgaa cgtgcgacac ttcccgatgc tcgaaggcct ccgggcgccc tatcggggct
     3661 agaggagtct cctgcagtcg ttgacccacg ttggcctatt gagcgccgca tgggccaccg
     3721 cgcaggccga ggagcgcctg acgaagagct cgacctcgcg ctcgccccgg atcctcggtg
     3781 tcatgggggg cccgctgatc gtcgtcagtg cgttcctcag ctggatcgcg cccgcgacct
     3841 cggccgaggg aacgagcatg cccgtttccc tcttctggag caccgagttc agcacgatgg
     3901 actccgatcc ttcattcttc gcctccgccg gattcatcgt catcctgatc ggagcggtga
     3961 cggtgaccgg gtgaccgata gggtcgcgtc ccagcgcact cgaatcggag gccaaggatg
     4021 cgcatcgacc agcgagaaca ctggtggtcc aaccggtggg gcgtgaggga ttcgaacccc
     4081 cgaccgatgg gttaaaagcc cactgctctg ccaggctgag ctaacgcccc gtacgattct
     4141 acggcgcgcg gcggtagcgg ttcgtgatgg ggaggcggcg gtcgcgtccg aaggctttcg
     4201 gcgtgatctt cactccgggc gcggcctgac gccgcttgta ctccgatccg tcgatcatcc
     4261 gcaccacccg atccacgagt ggcgcgtccg ccccgtccgc cacgatctcc tcgggcgccc
     4321 ggtcctcctc cacgtaggcc tcgacgatcg ggtcgaggac ctcgtacggc gggaggctgt
     4381 cggtgtcctg ctggtccggc cggagctccg cggagggcgg cttggtcagg acccggtcgg
     4441 ggatcggggg gtcggtctgc tcgttccgcc accgggccag gtcgtacacg agcgtcttca
     4501 ggacgtcctt cagcggcgcg aagccgcctg ccatgtcgcc gtacagcgtc gagtatccga
     4561 ccgccatctc gctcttgttg ccggtcgcca gcacgaggtg gccgaacttg ttcgaggccg
     4621 ccatcagcag gttgccgcgg atccgggcct ggacgttctc ctcggccacg ccgggctccg
     4681 tcccctcgaa gatcggggcc agcgcgtcga ggtatgcctg gaacgtgtcg tcgatcggca
     4741 cgcggtccag gcggatccgc aggcggttcg ccagctcctc ggcgtcctcg aggctgtggt
     4801 ccgaggagaa gcgcgagggc atcgcgagcc cccacacgtg gtcgggcccc agcgcgtcga
     4861 cggccaccac ggccgtcagc gaggagtcga tcccgcccga cagccccagg accacgtcgg
     4921 tgaagccgtt cttccgcacg tagtcgccga gcccgagcac gagcgcgcgg tacacctcgt
     4981 ccggcccctc cggccagggc tcccgctcgg gatcggggag ccggggcttc cgctcggtcg
     5041 cttcaggcag gtccacaacg agcatgtcct cctcgaacat gtccgcgtgc cacgcgcgct
     5101 ccccgttcgg cgccacgacc atgctgcccc cgtcgaagac gagctcgtcc tggccgccca
     5161 ccgcgttgac gtagacgatc caggcgccgg tctcgcgagc ccggtcggcg cagatctcga
     5221 tccgctggcc gatcttcccg cggtggtagg gggaggcgtt gaggttgggg atgatctgca
     5281 cggcctctgc gtatccgtcg aacggcggtc ccggcttcca cgcgtcctcg cagatcgaga
     5341 tcccgagctc cgcatcggcg acccgcaggg cgcagccctc cccgcccggg acgaagtagc
     5401 gggcctcgtc gaacacgccg tagttgggca gcttgtgctt gtggtagcgg gcctcgaccc
     5461 ttccgtcacg gagcaaggcc gccgcattgt gcatcccaag gtcggtccga tccacgaacc
     5521 cgacgaggac ggcggtcgtc tcccccgacc gtcgcgcgag ctcctggagc gcctcgaggt
     5581 tgtcctcgac gaacgcgggc ctcaggagga ggtcctcggg cgggtacccg gtgatcgcca
     5641 gctcggggaa gacgacgagg tcggcgccct ggcccgcggc ctcgacggcg ccggccgcga
     5701 ggcgctcgac gttcccctcc aggtcgccga ccgtcgtgtt cagctggccg agcgcgatgc
     5761 gcatggccac agcataggcc cgctcagagc acgggcaggt agcgctccag ctcgtacggc
     5821 gtgacgtacc gcttgtaggt gtcccactcc tcgcgcttgt tgcggatcag gaactcgtgg
     5881 acgtgatcgc cgagcgtctc gcgcagcagc tccgagtcct ccgccgcgcg gatcgcgtcg
     5941 tggaggtcct ccgggaggtg ctcgatcccg gcctcgcgcc gttcctcctc ggacatctcg
     6001 tagatgttgt tggaggcctc cggcgggatc tcgtactcct cctcgatccc cttgaggccc
     6061 gcggccagca tcgcggcgaa cgcgaggtac gggttgcagg cggggtcggg cgagcggagc
     6121 tcgatccggg tggccgactc cttgcccggc ttgtacatcg ggacgcggac cagcgccgac
     6181 cggttgcgcc gcgcccagca gatgtagacc ggcgcctcgt acccgggcac cagccgcttg
     6241 tagctgttca cccactggtt gaggacgagg gtgatctcgg gcgtgtgccg aaggatgccc
     6301 gcgatgtagc ccttcgccgt cttcgacagg tggtactcgt cggtgccgtc gaagaacgcg
     6361 ttgcggtcgc cctcgaagag cgactggtgc gtgtgcatcc cgctgccgtt cacgccggcc
     6421 accggcttcg gcatgaacgt ggcgtggacg ccgaactcct gcgcagtctc cttgaccgtg
     6481 agccggtagg tcatgacatt gtcggccatc gtcagcgcgt cggtgtagcg gaggtcgatc
     6541 tcgtgctggc tcggcgccac ctcgtggtgg acgtactcga ccgggatgcc catcgcttcg
     6601 aggtatccga cggtccgctt ccggtagtcg gtggccacgt cgcggggggt gaggtcgaag
     6661 tagccgccgc ggtcgaggaa ctcggtgccg ccgtcgtcct ggaagtagaa gtactcgagc
     6721 tcggggccga tgtagaacgt gaacccgaga tcggccgccc gctggagctg gcgcgtcagc
     6781 acccaccgag gatcgccctc gaagggcgtc ccgtccgggt tgacgacgtc gcagtacatc
     6841 cgggccacgc cctcctccga ccgccagggg atcacctgga aggtcgtcgg gtcggggatc
     6901 gcgaccatgt ccgactcctg gattcgtgag tacccctcga tcgaggaccc gtcgaagccc
     6961 atgccttccg tgaaggcggt ttcgagctcc tggggcgtga tcgcgaacga cttgaggaac
     7021 ccgagcacgt ccgtgaacca caggtggatg aacctgatcc cccgctcctc gacggtgcgc
     7081 aggacgaact ccgcttcctt ggtcagtgtc atgcggccag ggtaggcggt cggcgacgcg
     7141 ccgtggttac ggctgggtaa accctggagg atcgcgcgcg gtttacgatg gccgcgtgga
     7201 gaacgagcag gtgctgaacg aggtcgagga gcggggtatc cgcttcgtgc ggctctggtt
     7261 cacggacgtc ctgggcttcc tgaagtcctt ctccatcccc atcgaggagc tggagaaggc
     7321 tttccgtgag gggatcggct tcgacgggtc ggcgatcgag ggcttcgctc gcgtggagga
     7381 ggccgacatg ctggcccgcc cggacgcggc gaccttccgc gtgctgcccg tcccgcggga
     7441 ggagccggcg gccaggatgc tgtgcgacat cacctatccg gacggcgccc cgttcgaggg
     7501 cgacccccga cacgtattgc gccgcaacct gtctcggctc gatggccggt cgttccacgc
     7561 cgagcctgag gttgagttct tcctctccgg ctcggaggag cggttccagc cgctcgacca
     7621 cgggtcctac ttcgacctga cggcgagcga cgcggccgag gagttccggc gccgggccgt
     7681 ccccgtgctg gagacgctgg ggatcgagat ccggatgctc catcacgagg acgccccggg
     7741 ccagcaggag atcgacctgg cgccagggga cgccctccgc gtggccgacg acctgatcac
     7801 tctgcgcctg ctggtgaagg aggtggcgca ggagctgggc ctccacgcct cgttcatgcc
     7861 caagcccgcc ccggggatcc agggctcggg gatgcacgtg cactaccggc tgctggaggg
     7921 ggagcggaac gccttcttcg acccaaccga cgaggccagg ctgtcgaagg aggggcggtc
     7981 gttcgtggcg ggcctgctgg agcacgcccc ggctatgacc gccgtcacga accagtgggt
     8041 caacagctac aagcggctcg tccccgggtt cgaggcgccg atccacgtgt cctgggcccg
     8101 gcggaaccgg tcggcgctcg tgcgcgtgcc ggccgatcgg ccggacgacg aggcgtcctg
     8161 ccgcgtggag taccggggcg ccgacccggc ctgcaacccc tacctggcct tcgccgtggt
     8221 gctggcggcg gggctggacg ggatcgagcg cggcctcgag gcgccgcccg aggtccacga
     8281 ggatctggcc tcgctctcgg acgaggagct gcgcgcgctc ggcgtgcggt ccctccccga
     8341 caacctccac gacgcgatcg ccgagatgga gcgctcggac ctcctggccg aagcgctggg
     8401 cgagcgcctc ttcgagtggc tgatccgcaa caagcgcgac gagtgggagg cgtaccggac
     8461 gtacgtcacc ccgttcgagg tcgagcgcta cttcccgctc ctctagtgcg atcgcccgag
     8521 gccctcggcc gggcgctggc cgccgccccc gaccccgaaa tggcacgggt cgccctctcg
     8581 cgcgtggggg aggacccgcg ggtccgagtg gcgctcgagg agcccggggc cgcggagatg
     8641 gccgtccggc tgctcgggtt ctcgggtccc gctgcggact tcctggtggc gtacccggag
     8701 gaggcggggc tcttctccga gctgggcccg cggtcgaggg aggcgctgct cggggaagcg
     8761 cgggcggacc tggagcggct gggaggcgag gccggcctgc gtcggttccg ccgccgggcg
     8821 ctgctccgcg tcgcggcccg ggacctgggc ggcgcggtgc tggaggaggt cgtggccgag
     8881 gtgacctcgg tcgcggaggt ctgcctggcg ctggccgtgg aggccgccgg cgccggcggt
     8941 ctggccgtga tcgggatggg gaagctgggg ggcgaggagc tgaactactc ctcggacgtg
     9001 gacgtgatct tcgtccacga agaggcggcg cccgaggagg cggcccgggc cgcgtcgcgg
     9061 ctcatcgccc tgctctcgga gcccactcgg gacggggtcg ccctcagggt ggacgccgcc
     9121 ctgcgccccg agggacgggc cggccctctc tcgcgctcgc tcgcctcgac cgtcgagtac
     9181 tacgtgcgcc acgctgcgac ctgggagcgg caggccctcc tcaaggcgcg gcccgtggcc
     9241 ggggacctcg agctcgggcg gaggctgctg gccgagctgg acccggtgat ctacccggag
     9301 cacctcgacc ccgaggcaat cgacgacatc cgggcgatga aggcccgtct cgaggatcac
     9361 gttcgggcca ggggcaagga ggagtcagag gtcaagcggg ggcggggcgg gatccgggac
     9421 gtggagttcg ccgtgcagct cctccagctg gtgcacggga gacgggacgc gaggctccga
     9481 tcgcgcggca ccctccgcgc gctggcggac ctggcggagc aggggtacgt ggcccccggc
     9541 gacgccgagg cgctggcggg ttcgtatcgc ttcctgcgga ccgtggagca ccgcctccag
     9601 atcgcccggg acctccagac gcacgagctg ccggaggacc ggccgtcccg gcggcggctg
     9661 gcccgatcgc tgggcatcgc atcgcccgcc gagctcgggg ccgcctacga gcgccacgcg
     9721 gaggtcgtgc gcgggctcca cgagcgcctc ttctaccgtc cgctgctgga ggcgttcgcc
     9781 gggccgcgcc tgccgcgccc ggcggaggag cgggctcaga ccgaggagct gctcaccgcc
     9841 ctcggcttcc gcgagccccc ggccgcctat gagcggctgg accgcctgat ggacgcggcc
     9901 tcgcggcggg gccgggtgct ggagggggtg ttcccggtgg tggctcccgc cctctcggcc
     9961 gcccccgacc cggacaaggc gctcgttcgc ttcgaccgcg tggccgacgg gctggacgac
    10021 gagggcgccg acctgctggc cgcggacccg acggtggcgc ggcggctggc gctgctggtg
    10081 ggggcctcgg gctgggcggc cgacttcctg gccgcccagc ccgaggccgc ggtgctgctc
    10141 gaggggcgcg atcgcccgga cgcccgcccc cgggagcggc tggtggcggc cgcgggatcc
    10201 tacgtcgccg gggagctccg cgtgccggcg tcgggccgga ccttggcgcg gatcgccgac
    10261 gacgtggttg cggaggccct gcgagcggcc cagcctgagg tccccatcgc ggtcatcgct
    10321 ctgggcaagc tggggggcga ggagctgaac ttcggctcgg acttggacgt ggtcttcgtg
    10381 tacgacgggg agggcgagga gccgttccgc gaagccgtcc gcgccgccga ggccacgctg
    10441 gaggggatcc gggaggcggg gttcgagccc gatccggatc tgcggcccga ggggaagaac
    10501 ggaccgctgg cccgctccct ggcctcgttc ctcgagtact ggcagcggtg gggccagacc
    10561 tgggagttcc agtcgctgct gaaggcccgg gccgtggcgg gggaggaggc gctggctcgg
    10621 cgctggctct ccaacgccga ggacctcgcc tacccggagg cgctcccgct cgatcgtgag
    10681 gcggagatcc gcaggatgcg ggtccggatg gagaaggagc gggtgcggcc ccccgaggcg
    10741 gcgcgcttcc acttcaagct cgggcacggg gggctggccg acgtgacctt cgccgtcgag
    10801 ctggcccaga tgcggcacgg ccggctgcac ccccccgtcc gccgccgaca cacgctggag
    10861 gcgctggagg ccctggcctc cgaggggctg atcgaggacg gcgtggcgct ggccctcggt
    10921 gaggcctacg tgttcctcaa cgaggtgaag aacgcgatgg agctcgacgc gcggcggccg
    10981 gcggacgcct tgccgcccac gccggaggcg caggtggcgc tggctcgcct gctcgggtac
    11041 gaggagcacc cccggcaggc cttcctggaa aagtatcgtc gggtcacccg ccgagcccgc
    11101 cgggcgatgg agcgggtctt ctatggggag gagccatgag gaccgtcgcg ctgatcctcg
    11161 ccctgacggc cgtggcgtgc gccgaggacg gggccggcgg gctcggtgcc gccccgtccc
    11221 ctgggttcga ggtcgtggag cgcggcaccg agcccgccgg ccccatgatc gagccctcgg
    11281 tcgtcttggt ccgaggcgac ctccaggcgg aggccgaggc ggcgaacgcc cccgccgagg
    11341 gcgccgccga actgatccga tcctgggacg agctcgacga gcgggccctc ctcgtcgtct
    11401 acggtgggac cgtgcccgac gccgggacgt cggtccgggt cgacgacgtc gcgctggagc
    11461 aggacgcacg ccacctgatc gtcgccgcga cgctcgagtc tgaggagccc gcgctccagg
    11521 tcgtctcgat cgcctgggcc gcggtctcgc tcgacgccga cctcgtagcc cgcgccgata
    11581 cctgcgcgct cgtcctggac ggccgcgagg tccccgacgc ggcgtgctcg gtggcgatcc
    11641 cctacgcgtc gaagtagcgg tagaactcgt acgggtgcgg ccgcagccgc agctcgtcga
    11701 tctcgttcct gcgcttgtag tcgatccagg tgtcgatcac gtcttgggtg aacacacctc
    11761 cctccagcag gaacgcgtgg tccacctcga gcgccgcgat cacctcgtca agcgagccgg
    11821 ggacctgccg cacgtcggcc gcctcctcgg gcgggaggtc gtagaggtcc ttgtcgatcg
    11881 ggtccggcgg ctcggtcttg ttcttgatcc cgtcgatccc cgccatcagc atcgcgctga
    11941 acgcgaggta cgggttcgcc gtcgggtcgg gggagcggaa ctcgatccgc ttggccaccg
    12001 gcgtcttgta gtagacgggg atccggatgc aggccgagcg gttccgctgg gagtacacga
    12061 ggttgatcgg cgcctcgtac ccgggtacca gcctgcgata ggagttcgtg gtcggcgcgg
    12121 cgaacgccag gagcgccggc gcatgctgca ggagcccgcc gatgtaccac cgtgccgagt
    12181 ccgacaggcc cgcgtacccc atctcgtccc agaacaggtt ctcctcgccg gaccagaggc
    12241 tctggtgcgt gtgcatcccg gacccgttgt cctcgaagat cggcttcggc atgaacgtca
    12301 cggtcttgcc ggccgcgaga gccgtgttct tgacgacata cttgtaggtc atcaccttgt
    12361 cggccgtccg gagcagggtg tcgtagcgga tgtcgatctc ggcctgcccg gcggtcccga
    12421 cctcgtggtg gtgcacctcg acctcgatgc cgaccttctc caggttcagc atcatctcgg
    12481 tgcgcaggtc ctggtagtgg tccatcggcg ggacggggaa gtacccctcc ttgtagcggg
    12541 gcttgtaccc cttgttcccg ccctcctcgt cccggcccga gttccacacg ccctccatcg
    12601 catcgatgtg gtagtagccc tcgtgctggt tctggtcgaa gcggatcccg tcgaagatgt
    12661 agaactcgca ctcagggccc cagtaggagg tctccgcgat ccccgtctgc tggaggtaga
    12721 gctcggcctt ccgggcgatg ccgcgtgggt cgcgtgtgta catctcgccg gtgaccgggt
    12781 ccttcacgaa gcaggccagc acgagcgtgg ggcggggacg gaaggggtcg acgtaggcgg
    12841 cgccggcgtc cggcagcagc agcatgtccg actcctggat ctcctggaag ccacggatcg
    12901 aggacccgtc gaacccgaac ccgtcctcga agctctcctc ggtcagctcg gacacgggga
    12961 cggagaagtg ctgcatcagc ccggggagat cgcagaaccg gaggtcgacc acctccgctc
    13021 ccgcatcctt cgccatcgcc aggacgtcct tcggcgtgct cacgcgcgac cctccttcgc
    13081 tcggatgacc ggaagcctac cggtcggtcg tcaccggagc gtctcccgcc cgttaactct
    13141 ctgtaacgga tcgagggttg aggtatcgag ggttcgccgg taccctcggc agcatgggac
    13201 gtgagatgag gtttcgtgtc ctcgcgaccg cgctggccgc gacgctcgcg ttctcgatca
    13261 cggggatcgg gacgcctgcg caggcggcta cctccatcgt gaggatgaag ggcacgagga
    13321 acaagttcag gccggtccac acgtacatcg ccaagggcga caaggtgtcg tggaggaaca
    13381 tgtcgaggcg cgtgcacgac gtgacggcgt acggtggcgg ctggaccttc tccaaggtgc
    13441 tgtccccggg ccagaggacg gcgaggaggt tcaggaagcg ccgcacctac aggtaccgct
    13501 gcgtgcggca ttcagcgatc gtgaacaacc gctgcagcgg catgtgcggg ttcgtgcacg
    13561 tcgtggcgta gccgtcagaa cgtcgcttcg aagcaggccg agatccggga gccgtcctcc
    13621 agcgtggcgg tcgagtcgaa ccgccacgtc ccgggggtga gcctcgtcgt ggccgcgaag
    13681 tgccccggcg tcagcagtcg ctccgagggg aggacgagcg gctccacgcc caccgggccc
    13741 gcgaggatct ccggctcctc gggcacccgg agctccttgc cgccggcgtc caggaagagg
    13801 aagtggatct cggtgcgcct cctgccgctg ggcaggaggt agccctggct cgaggttcca
    13861 tcttccaact gcaggatgta ggtggggatg ctcacggtgc ccacctcgag ctcaaccagg
    13921 tgggtctcgc agagggtggt cacgcggacc ggcacccccg tcgtgccccc gtcccgcccc
    13981 acgcgcaccg tgagctgcca cgggcccgag atggcgagcc cgtcgccctc gccctcgtag
    14041 cgcccctcgc cgacggcctc gaggtcgagc gtcgactcct tcccggaccc ggcaagcggc
    14101 acgaagtcga tcgcgacgtc cgcgtcctcg acgccctctc cgccctcgag gatcacagcc
    14161 tcgaagtggt tcggccccgg tgtgcccggg ctcaccccga ggcgcacccg cagtccgccc
    14221 gtggcctcgg tggccgagac ctgcagcggc gcgggagctc ggggcgtctc ggggatcgag
    14281 gcgggcggca ccagcgtggc cagcgcggcg gccgcggtga gcgcgccgag agctaccacc
    14341 agctcccctc cggagacgag ccggaatcct cgcagctccc gcgatgctcg cggcaccatg
    14401 cggtagcggt tgaaggcccc gaagccggcc agccccagga ggagccccga cttggcaacg
    14461 acgaaccggc cgtagccagt gctccagagc gccgaccagc cccccacctc gtccagcgcc
    14521 cggaacagtc ccgagagcgc cacgaccgcc agggcgtacc cggccaaccg ggagtacctg
    14581 cggaccgccg cgcccttgac ctcgtccggc gccccccgga ccccgatcac caggctcgtg
    14641 aggccgccca cccacagccc agccgcggtc acgtgggccc actgcgaggc gacctggggc
    14701 cagggcggct cgccggcgcc ggcatgccct cccgagacgt gcgccagcac ggcggccgct
    14761 ccgagcacgg ccccggtgag caggcgccgc gtcggcgacc ccagcggcac ggcgatcgcg
    14821 gcggcggcca gcagcgcgag gcccgcccgg aggatcgcgg agcgcccgat cgccgtgccc
    14881 accgcatccg cgatcgaggc gcccgccgcg ctggcctggg ccaggcccac cgtcgcgccc
    14941 gccagcgtgg cgagcaccgc gcccgccagg gccagtctcc gaagcacgac cgggggctgc
    15001 gggaaggcca cggaccccac cagcgacgcc ccgaggagaa gcgcgaaccc aatgtagagg
    15061 agcgtcctgg cggtcacctc gaagggcgtc gcggtgggcg cggccgccac ttcctgctcc
    15121 tcggcccccc gctccggggg gtccacgccg atgccgaacg cgaagatgcc ggccgtcgga
    15181 tgcccgtcca cggtcgagac gacctgccag gtcaccgtgt agacgccgcc ctgctccagc
    15241 tgccccaggt cgaccgagag gatcgtgggc tcgctcgggt ccagccggct ctcgccctga
    15301 tggacctcct gccctccttc gtcctcgacc acgatctggg agagcgacgg ctcgagcggc
    15361 tcggtgaacg tcagcgtgat cgtcgagggc gcttcgccga ggttggcccc gtccgcggga
    15421 tcggcgaaga gaaggagggc gtggccggag gcgggaccgg cgagggccag gatcgcgacg
    15481 cccgccgcgg tgagcgtgac cgcgaggcgc agggtcgagc ggaggcggag gagccggccg
    15541 acgcccggga tcacgcgctc agtctagtgg gcgttcgccg aacgaggcgc ggtagcccac
    15601 gctgcggacc gtctcgatca ggtgctcgtg ctccgggccg agcttggctc gcagccgccg
    15661 gacgtggacg tcgaccgtcc gggtgcctcc gtagaagtcg tagccccaga cctcgcggag
    15721 cagctgggcc ctggtgaaga cgcgggcctc gtgctgggcg aggaagcgaa gcagctcgaa
    15781 ctccttgaac gtgaggtcga gggcacggcc cccggccgtg acgcggtagg tcagcgtgtt
    15841 gatcgccacg ggccccaacc tgagcacgcc ctcgcccgtc tctcccacgc gggcccgcag
    15901 cagctccacg cggaggcgga gctcgtcctg cccggtctcg gggtggacga agtcgtcggc
    15961 cacctcctgc cagggcagca tcccgagctg atcccgaccc aggaccacga gagacggggg
    16021 gccgcctcgg gcccgcagct cccggaggag gccgaagcag cgctcggggt cggggacggc
    16081 gtcggccact accaggtcgg ggctgaggtc ggggacgcgg gagagcccgg tggcgtccag
    16141 cgagtcggtc ttgacgtctg tccccagggc gtgcgcggcg gggagcacgt cgccggggcg
    16201 gcgatccgtg aggatgagga gccgtcgcac cggaacgagc gtagcatcgg ccccgcgatc
    16261 gggggaggta cccttgcctc cgtgatcgcg cgcctcctct cccgccgata tcggtcggct
    16321 gccccgttcg gggcgacgac ggccgacggc gtcccgctcg tcggggtccg cctgggcgag
    16381 ggtccggccg cgctcgtcct ctgccacggc ttcatgggat ggcaccgcaa ggcgagcatc
    16441 gtctcgttcg cggagcggct ggccgagcgg ttcctcgtct acgtcttcga cctgcggggg
    16501 cacggcggga gcgccggcgt ctgcacgtac ggcgaccgcg agtacctgga cgtggacgcg
    16561 gtcgtggggc tggcccgccg cgagaggggc ctcccggttg ccaccctggg cgcctccatg
    16621 ggcgggatcg cggtgatccg tcaggcggcg ctcggcgagc ctcctgacgc tgtgatggcg
    16681 gtgtccacgc ccgcacgctg ggaggggcac gccaccgccg cggtccgacg ggtgacccgg
    16741 atgacggcca cgccgtcggg ccgccggatg atgcgggccc tgggcttccg gctgaccgac
    16801 acctgggggt cccc
//
LOCUS       DASUTZ010000262         2984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_620940, whole genome shotgun sequence.
ACCESSION   DASUTZ010000262 DASUTZ010000000
VERSION     DASUTZ010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620940"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..168
                     /locus_tag="VF097_11240"
     CDS             <1..168
                     /locus_tag="VF097_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005270350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEX6263408.1"
                     /translation="FEEKVRREESRVKGQAELAASSLDAQFETLDDATTDAEVEARLA
                     QLRSGAKEGEE"
     gene            186..1163
                     /locus_tag="VF097_11245"
     CDS             186..1163
                     /locus_tag="VF097_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020127328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX6263409.1"
                     /translation="MKAIVQDEYGPPDVLELREVDPPAIREREVLVRVRAAAVHPGDL
                     FLMEGWPRVLRVVYGLRRPKRNIPGFDVAGIVEVVGAEVRHLHPGDEVFGNGRGTCAE
                     YTSASEGELAPKPDDLSFEQAAAVPTSAVAALRGIRDAGKVRPGQKVLINGASGGVGT
                     FAVQIAKALGAEVTAVCSTANADLVRSLGADHVIDYTREDFTRGGRRFDLILDNVASH
                     PLSETRRALAPGGVLLPNSGRSGGAWFGPLGRIALAFVLSPFIRGQGRQFVATVKAED
                     LVDITEMIEAGRVRPVVERTYPLGETPDALRHVARGHTRGKVVISVSEP"
     gene            complement(1160..1588)
                     /locus_tag="VF097_11250"
     CDS             complement(1160..1588)
                     /locus_tag="VF097_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019945705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6263410.1"
                     /translation="MTKLHSYLNFAGNAEEAFEFYRSAFGGELSSLVRFRDLPMEGVD
                     IPEEDRGKIMHIALPIGDEDLLMASDALRSLGQEVVQGNNVYVSVHPGSKEEADRIFG
                     ALSEGGEIEMPIADQAWGDYYGSLKDKFGIRWMVNYNPSS"
     gene            complement(1585..2487)
                     /locus_tag="VF097_11255"
     CDS             complement(1585..2487)
                     /locus_tag="VF097_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6263411.1"
                     /translation="MQKITPHLWYDREAQEAAELYSSILPDSRVTNVTTIHDTPTGDT
                     DIVSFELMGQPFMAISAGPLFRFTPAVSFLISCGTKEEVDAIWERLSERGTALMPLDS
                     YPFSDRYGWTEDRYGLSWQVMLADEEPAQRITPTLMFVNEVSGKAEEAIAFYASVFPD
                     SRVGPILRYGADEAPDEEGTIKHAAFRLAGQEFAAMDSALGNSGFNEAISFLVSCGTQ
                     EEIDDYWDSLSAVPQAEQCGWLKDRYGLSWQVVPSAMDEMMATGTEDQIARVTQAFLQ
                     MKKFDLAELQRAYEGSEAPVGGEA"
     gene            2713..>2984
                     /locus_tag="VF097_11260"
     CDS             2713..>2984
                     /locus_tag="VF097_11260"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX6263412.1"
                     /translation="MADALTASRALTAVLLLWLVGSGRLGAAAAALAAAWASDALDGT
                     LAAAAGGGTRLGPWDLPVDVLVGAAVLVGLGLYGSLPWPLVIVVLVV"
BASE COUNT          459 a         1021 c         1024 g          480 t
ORIGIN      
        1 ttcgaggaga aggtccggcg cgaggagtct cgcgtcaagg gccaggcgga gctggcggcc
       61 tcgtcgctcg acgcgcagtt cgagaccctc gacgacgcca cgaccgacgc ggaggtcgag
      121 gcccgtctcg cccagctcag aagcggggcg aaggaggggg aggagtagct ggacccctcg
      181 atccgatgaa ggcgatcgtc caggacgagt acggcccacc cgacgtcctg gagctccgag
      241 aggtcgaccc gccggcgatc agggaacggg aggtgctggt acgcgtccgc gccgccgcgg
      301 tgcatcccgg cgacctgttc ctcatggagg gatggccgcg cgtgctgcgg gtcgtctacg
      361 ggctccggcg gccgaagagg aacatcccgg gcttcgacgt ggccgggatc gtcgaggtag
      421 tgggcgcgga ggtaaggcac ctccatcccg gtgacgaggt gttcgggaat ggcaggggca
      481 cgtgtgccga gtacacctcc gcctcagaag gcgaactggc cccgaaaccg gacgacctct
      541 cgttcgagca ggccgcggcc gtgcccacct ccgccgtcgc cgcgctgcga ggcatccgcg
      601 acgcgggaaa ggtgcggccg ggccagaagg tgctgatcaa cggggcgtcg ggaggcgtgg
      661 ggacgttcgc cgtgcagatc gcgaaggcgc tcggggccga ggtgaccgcg gtctgcagca
      721 ccgccaacgc ggatctggtc cggtcgctcg gcgccgacca cgtcatcgac tacacgcggg
      781 aggacttcac ccggggcggg cgccgattcg acctcatcct cgacaacgtg gcgagccatc
      841 cgctctccga gacgaggcgg gcgctcgctc ccggaggcgt cctcctcccg aacagcggca
      901 ggtcgggagg cgcgtggttc gggccgctgg gccgcatcgc cctggcgttc gtcctgtctc
      961 cgttcatccg cggccagggg cgccagttcg tcgcgaccgt gaaggccgag gatctcgtcg
     1021 atatcaccga gatgatcgag gcggggaggg tccggccggt cgtcgagagg acctacccgc
     1081 tcggcgagac tccggacgcc ttgcgccacg tggcgcgggg gcacacgcga ggcaaggtcg
     1141 tcatcagcgt gagcgagccc taagagctgg ggttgtagtt caccatccac cggatcccga
     1201 acttgtcctt caggctgccg tagtagtcgc cccacgcctg gtccgcgatc ggcatctcga
     1261 tctctccacc ttcggacagc gcgccgaaga tccggtcggc ctcctccttg ctcccggggt
     1321 ggaccgagac gtagacgttg ttgccttgaa cgacctcctg tcccagcgac cgcagcgcgt
     1381 cactcgccat caggaggtcc tcgtccccga tcggcagggc gatgtgcatg atcttgcccc
     1441 ggtcctcctc ggggatatcc acgccctcca tgggaaggtc cctgaaacgc acgagcgacg
     1501 agagctcacc gccgaacgcg gaccggtaga actcgaacgc ctcctcagcg ttgcccgcga
     1561 agttgagata gctgtggagc tttgtcatgc ctcacctccc accggtgcct ccgacccttc
     1621 gtacgctcgc tgcagctccg cgaggtcgaa cttcttcatc tggaggaacg cctgggtgac
     1681 tctcgcgatc tggtcctccg tgcccgtcgc catcatctcg tccatggcgg aaggaaccac
     1741 ctgccaagag agcccgtacc gatccttcag ccagccgcac tgctccgcct ggggcaccgc
     1801 ggacaggctg tcccagtagt cgtcgatctc ctcctgcgtc ccgcaactca cgaggaagga
     1861 gatggcctcg ttgaagccgg agttcccgag ggcgctgtcc atggcggcga actcctggcc
     1921 cgccagccgg aaggccgcgt gcttgatcgt gccctcctcg tcgggggcct cgtccgcgcc
     1981 gtagcgaagg atcgggccga ccctcgagtc ggggaacacc gatgcgtaga aggcgatggc
     2041 ctcctccgcc ttgccggaca cctcgttcac gaacatgagg gtcggcgtga tgcgttgtgc
     2101 gggctcctcg tccgcgagca tgacctgcca cgagagcccg taccggtctt cggtccagcc
     2161 gtagcggtcg ctgaacggat acgagtccag gggcatcaga gccgtgcccc gttcggagag
     2221 gcgttcccag atcgcatcca cctcctcctt ggtgccgcag gagatcagga acgacaccgc
     2281 gggagtgaac ctgaagagcg ggcctgcgct gatggccatg aacggctggc ccatcagctc
     2341 gaacgacacg atgtccgtgt ccccggtcgg ggtgtcgtgg atcgtggtca cgttcgtgac
     2401 cctcgaatcc gggaggatgg acgagtacag ctcggcggcc tcctgagcct ccctgtcgta
     2461 ccagaggtgt ggcgtgatct tctgcatgcg gcctcctcga tcgagcgtac gcctgtaaga
     2521 cggccccggc ctcgaggact catcgctcgc ctcctaggat gcccgccaga catcctcatc
     2581 gtcggacgcg agcaggcacc tgatcacgga caagcccagc gatcatggaa cgacggaccc
     2641 actcgccggt gtaggctccc cgggatggcc ggaacaccca cgactgcggg atcgagcact
     2701 agactcgccc gtgtggccga cgcgctgacg gcgagtcgag cgctgacggc ggtcctcctc
     2761 ctgtggctcg tcgggagcgg gcggctcggc gcggcggccg cggccctggc ggctgcgtgg
     2821 gcgtccgacg cgctcgacgg cacgctggcc gccgcggccg gcggcggaac ccgtctggga
     2881 ccctgggacc tccccgtcga cgtgctcgtg ggggccgccg tgctggtcgg gctcgggctc
     2941 tacggctcgc tcccctggcc cctcgtcatc gtcgtgctgg tcgt
//
LOCUS       DASUTZ010000263         4783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_103376, whole genome shotgun sequence.
ACCESSION   DASUTZ010000263 DASUTZ010000000
VERSION     DASUTZ010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103376"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VF097_11265"
     CDS             <1..594
                     /locus_tag="VF097_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HEX6263413.1"
                     /translation="LEADNTKGYWQEHRHVYDELVRAPMEALLEELAPEWGDWKIFRP
                     YRDIRFSRDKSPYKTHIGATIGDGYVQLSADGLAAGCGMYRMAKDQLERYRAAVSDES
                     TGRELVSLVEAVRASGLEITAHDELKSAPRGYPKDHPRIELLRLKGLIAWRQWPAAVW
                     MGTPWAKDRIVDFLSRSRPIGDWLRANVGPSTLPPER"
     gene            655..1455
                     /locus_tag="VF097_11270"
     CDS             655..1455
                     /locus_tag="VF097_11270"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX6263414.1"
                     /translation="MAIALFRVGIVDSDKDFTERLELEDALSSMHRLIRRRGNGPYAA
                     RIRRRGHTWSPRRLDGAAIEQKVREWLPTLDVGGVVAVRRSDGGVVIIRVAKPKPEPR
                     VAFDRVPVPCGGYQDGWGAPRSGHDHQGIDIFALAGSPITAPFDGTLRFSFRGTSAGW
                     AADIVAADGRGIVRNFHCQKTRIRPDGPVKSGDQCANVGNTGNALHTPAHSHFEFHAK
                     LRSFDAVVSGNVAKEPCSSDGTACDPFAELNAAYGKIGGGTRGRGRPC"
     gene            1463..3139
                     /locus_tag="VF097_11275"
     CDS             1463..3139
                     /locus_tag="VF097_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX6263415.1"
                     /translation="MAASYPTYDVLVIGSGFGGSVAALRLAEKGYRVGVLEAGRRFGP
                     RDFATRNWNLRRYLWLPRLGLRGIQRLTLLRDALVLSGAGVGGGSLVYAGVLYDPPDP
                     FWDDPGWSRVADWRHELAPHFDQAKRMLGAAANPFDTPADRVLHQVAARLDANDTHHP
                     TTVGVHFGEPGRRVTDPYFGGAGPARAGCIGCGGCMVGCRFDAKNTLDRNYLWLAEGL
                     GAVIHPETEAVDVNPLPGGGYEVETRRPGSWSADRRRFRARQVVFAAGVLGTLKLLLR
                     LRETGRLRRLSARLGHLVRTNSEVVVGAVARRPDVDYSTGVAITSSIQPAPDTHVQVV
                     RYPRGSNFMGLIGTILVDRDERRSRQLSFLAQALRRPVRFVRSLSAYRWSERSVILLV
                     MQSRDNSLRLVLRGRRLTSVAGEGTPSTRYIPVANRAARLAAEIMGGEPAGALNEALL
                     DVPVTAHMLGGARIGTSSEDGVIDPYHRVFGHPGLHVMDGSTIAANLGVNPALTITAL
                     AERACSFWPNAGEADPRPELGRYERIAPVPPRRPAVPEGAPASLSYNDGR"
     gene            3245..4330
                     /locus_tag="VF097_11280"
     CDS             3245..4330
                     /locus_tag="VF097_11280"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX6263416.1"
                     /translation="MDAYIGVGHGRTPDGTFDPGAVSGDLVEHDLAVSVVDAYADAMR
                     RSGARVTDESNSGPGHDPNFVGSAREANRLRVKYADEVHFNASATHAGTGTEVWVHGG
                     TSEANKRACRAMAAAISRVLRIPNRGLKLGTFSFLTSTNMPSAIIEVAFVDNTADRRA
                     IRRKDALEEVGDAIAEARCRFLGVTPAQPDPGRRGVSIAGRRPATSRRKAHAWARSKG
                     AAPVFHEIIDAGWREARDIGIDAAVFVAQAAKETGFGRFGGVIDASFHNSCGLKTTAG
                     GDNDDPAAHQRFRDWEMGTRAHCAHLALYAGVLTPAEAKRLGDPRAFASIHRVAPTVQ
                     ELGGKWAPARDYGRSLVRDLYRPLRRF"
     gene            4394..4732
                     /locus_tag="VF097_11285"
     CDS             4394..4732
                     /locus_tag="VF097_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEX6263417.1"
                     /translation="MAYDEDLAARVRDVLAEVDGSTDEREMFGGIAFMVGGHMCCGVT
                     AEDLMLRLGPDRAEEALDEPHVRPMDFTGRPMKGYVYVSAKGLETEEDLARWIGLARA
                     FVAELPPKGR"
BASE COUNT          718 a         1630 c         1750 g          685 t
ORIGIN      
        1 ctcgaggccg acaacacgaa ggggtactgg caggagcatc ggcacgtcta cgacgagctg
       61 gtccgcgctc ccatggaggc cctgctcgag gagctcgccc ccgagtgggg ggactggaag
      121 atcttccgcc cctaccgcga catccgcttc agtcgagaca agtcgccgta caagacccac
      181 attggcgcga cgatcggcga cggctacgtc cagctgagcg ccgacggcct cgccgccggc
      241 tgcgggatgt accggatggc gaaggatcag cttgagcgct acagggcggc ggtgagcgac
      301 gagagcacgg gccgggagct ggtctctctg gtggaggcag tgagggcgag cggactcgag
      361 atcaccgccc acgacgagct gaagagcgcg ccgaggggtt atccgaagga ccatccgcgc
      421 atcgagctgc ttcgcctcaa gggcctgatc gcctggcggc agtggccggc ggcggtatgg
      481 atgggcaccc cctgggcgaa ggaccggatc gtggacttcc tcagccgctc gaggccgatc
      541 ggcgactggc tccgagccaa cgtcgggccc tccacgctcc ccccggagcg ctgagccgga
      601 ccagaggctc tctcgctcgg gaccggtcgc cgctccggtg ctagagtccg gccgatggcg
      661 atcgctctgt tccgcgtcgg gatcgtcgac tcggacaagg acttcaccga gcggctcgag
      721 ctcgaggacg ccctctccag catgcatcgc ctcatccgtc gtcgggggaa cggcccctac
      781 gcagcgcgca tccggcgtcg ggggcacacc tggtcccccc ggcggctcga cggcgcggcc
      841 atcgagcaga aggtccgcga gtggctcccc accctcgacg taggcggcgt cgtggccgtc
      901 cgccgctccg acggaggcgt cgtcatcatc cgggtggcga agccgaagcc cgagccgcgg
      961 gtggccttcg atcgcgtgcc ggtgccctgc ggcggatacc aggacggctg gggggcgccc
     1021 agatccgggc acgatcatca ggggatcgac atcttcgctc tcgcggggtc gccgatcacc
     1081 gcgccgttcg acgggaccct ccgcttctcg ttccggggca cgagcgcagg atgggcggcg
     1141 gacatcgtcg ccgcggacgg gcggggcatc gtccggaact tccactgcca gaagacgcgc
     1201 atccggcccg acgggccggt gaaaagcggc gatcagtgcg cgaacgttgg caataccggg
     1261 aacgccctcc acacgccggc ccactcccac ttcgagttcc atgcgaagct gcggagcttc
     1321 gacgcggtcg tctcggggaa cgtggcgaag gaaccatgct cctcggatgg gaccgcctgc
     1381 gatccgttcg cggagctcaa cgcggcctac gggaagatcg gcggcggcac ccgcggccgc
     1441 ggccgcccct gctaggaggg ccatggccgc ctcgtatccg acgtacgacg tcctcgtcat
     1501 aggcagcggg ttcgggggaa gcgtcgccgc actcaggctc gccgagaagg gctaccgggt
     1561 cggtgtgctg gaggcgggac ggcgcttcgg gccgcgagac ttcgccacgc gcaactggaa
     1621 cctccggcgg tacctgtggc tcccgaggct ggggctgcgg ggcatccagc ggctcacgct
     1681 cctccgcgac gccctcgtgc tctcgggggc gggcgtgggg ggaggttccc tcgtctacgc
     1741 cggggtcctg tacgacccgc cggatccctt ctgggacgat cccggatggt cgagggtcgc
     1801 cgactggagg catgagctcg ccccccactt cgaccaggcc aagcggatgc tgggggccgc
     1861 cgcgaacccc ttcgatacac cggccgaccg cgtgctccac caggtcgccg ctcgtctgga
     1921 cgcgaacgac acgcaccatc cgacgaccgt gggggtgcac ttcggcgagc ccggcaggcg
     1981 ggtcaccgac ccctacttcg gcggcgcggg tccggctcgg gccggctgca tcggatgcgg
     2041 ggggtgcatg gtggggtgcc gcttcgacgc caagaacacg ctcgaccgga actatctctg
     2101 gctggccgag ggcctgggag ccgtgatcca tcccgagacc gaggccgtcg acgtgaaccc
     2161 gctccccggc ggcgggtacg aggtggagac gagacggccg gggagctggt cggcagaccg
     2221 tcgccgcttc cgggccaggc aggtggtgtt cgccgcaggc gttctcggca ccttgaagtt
     2281 gctcctcagg ctcagggaga cggggcggct gcgacggctg tccgcgcgcc tggggcactt
     2341 ggtccggacg aactccgagg tggtcgtggg ggccgtcgcc cggaggccgg acgtcgacta
     2401 ctcgacagga gtggcgatca cctcgtccat ccagccggcg ccggacaccc acgtgcaggt
     2461 ggtccgctac ccgagaggca gcaacttcat gggcctgatc ggcacgatcc tcgtcgaccg
     2521 ggacgagcgg aggtcgcggc agctgtcgtt cctggcccag gccctgcggc gccccgtgcg
     2581 gttcgtgcgc agcctctccg cctaccgttg gtcagagcgg tcggtgatct tgctggtgat
     2641 gcagagcagg gacaactccc tgcggctcgt cctgagggga cggcggctca cctcggtcgc
     2701 cggggagggc acgcccagca ctcgatacat ccccgtcgcc aaccgcgccg cgcgcctggc
     2761 ggccgagatc atgggcggcg agcccgctgg ggccctcaac gaggccctgc tcgacgtccc
     2821 ggtgacggct cacatgctcg gcggtgcccg catcggaacc tcatcggagg acggcgtgat
     2881 cgacccctat caccgggtgt tcggccatcc cggcctgcac gtgatggacg gctccacgat
     2941 cgcggcgaac ctcggagtga acccggcgct cacgatcacg gccctggcgg agcgggcctg
     3001 ttccttctgg cccaacgcgg gggaggcgga cccccggccg gagctcgggc gctacgagcg
     3061 catcgccccc gttcctccga ggcgaccggc ggtccccgag ggcgccccgg cgagcctctc
     3121 ctacaacgac ggccgctgac ctccggcact cccccatccg cccgattgac cggtcgctcg
     3181 cacgctccta gccttgctcc actcgtcgtc ggaggggtgc cggcccgtat cgaggaggtg
     3241 cgccatggac gcctacatcg gggtggggca cgggagaacg ccggacggca cgttcgatcc
     3301 gggggcggtc tccggcgatc tcgtggagca tgacctcgcc gtctcggtgg tggacgccta
     3361 cgcagacgcg atgcgcagaa gcggggcgcg ggtcaccgat gagagcaact cggggcccgg
     3421 ccacgacccc aacttcgtgg gctcggcccg ggaggcgaac cggctccgcg tgaagtacgc
     3481 ggacgaggtt cacttcaacg cctccgcgac gcacgctggg accggcacgg aggtctgggt
     3541 gcacgggggc acctcggagg cgaacaagcg cgcgtgccgc gccatggccg ccgcgatctc
     3601 tcgggtgctc cgtatcccca accgtgggct gaagctgggg acgttcagct tcctcacctc
     3661 gaccaacatg ccctcggcca tcatcgaggt ggccttcgtg gacaataccg ccgaccgaag
     3721 ggcgatccgt cggaaggacg cgctggagga ggtgggcgat gcgatcgccg aggcccgctg
     3781 ccggttcctc ggcgtcacgc cggcccagcc cgaccccggc aggcgcgggg tctcgatcgc
     3841 cgggcgccgc cccgcgacgt ccaggcggaa ggcccacgcc tgggccaggt cgaagggggc
     3901 cgcccccgtc ttccacgaga tcatcgacgc gggatggcgg gaggcgcggg acatcggcat
     3961 cgacgcggcc gtcttcgtgg cccaggcggc caaggagacc ggcttcggta ggttcggggg
     4021 cgtgatcgat gcgagcttcc acaactcttg cggcctgaag accaccgcgg gaggggacaa
     4081 cgacgatccg gccgcacacc agcggttccg agactgggag atgggaacca gggcgcactg
     4141 cgcgcatctc gcgctctacg cgggagtgct gacgccggcc gaggctaaga ggctgggaga
     4201 tccgcgcgct ttcgcctcga tccaccgggt cgcgccgacc gttcaggagc tcggggggaa
     4261 gtgggctccc gcacgcgact acggccgaag cctcgtccgc gacctctacc ggcccctccg
     4321 ccggttctag gctcgccggg gagcccttcg tgggtggtga agggcatgat ggtgcgggat
     4381 tcgaggaggc acgatggctt atgacgagga ccttgcggcg agggtgaggg acgtcctcgc
     4441 ggaggttgac gggagcacgg acgagcggga gatgttcggc gggatcgcgt tcatggtggg
     4501 gggtcacatg tgctgcgggg tgaccgccga ggatctgatg ctgaggctgg ggcccgatcg
     4561 ggcggaggaa gcgctggacg agccgcacgt ccggcccatg gacttcaccg gccgcccgat
     4621 gaagggctac gtgtacgtct cagcgaaggg cctggagacc gaagaggatc tggcacggtg
     4681 gatcgggctc gctcgggcgt tcgtcgcgga gctccctccc aagggacgat gaacacgaac
     4741 ccggaggtcg accggtggct cgtggagcgg gatcatccgc tcg
//
LOCUS       DASUTZ010000264        11442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1785762, whole genome shotgun sequence.
ACCESSION   DASUTZ010000264 DASUTZ010000000
VERSION     DASUTZ010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11442
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1785762"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VF097_11290"
     CDS             complement(<1..423)
                     /locus_tag="VF097_11290"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase / HUMPS
                     family protein"
                     /protein_id="HEX6263418.1"
                     /translation="MSRLIVAADVPSVAAAEDLARSLAGTVGMVKVGLELFASEGPEA
                     VRRLRGHLPVFLDLKLHDIPTTVERASRVVGRLGAAMLTVHALGGPEMVAGAVRGAAE
                     GAAEEGMEPPEVIAVTVLSSLAEAAGAAPASLAFEASEA"
     gene            complement(398..1363)
                     /locus_tag="VF097_11295"
     CDS             complement(398..1363)
                     /locus_tag="VF097_11295"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEX6263419.1"
                     /translation="MARARVNLGVDLGGVQLPTPVLGASGTFGSGRELSPILDLSKVG
                     GIVTKSVTLRPVKGLPTPRMVETPSGMLNAIGLQNPGIDEFMEKEGPFIRRVGVPVFL
                     SIAGKSVEEFVQVAMRAQNIPGVVALEANISCPNVERRNQVFACHPDAAAEVVGAVAR
                     LSRLPVFAKLTPDVTDIAEVAEACVRAGAHGISLINTLLAMAIDVETFRPKLGGVTGG
                     LSGPAIRPVAVRAVYQVARALPEVPIIGMGGIRSARDAVEFLLAGAWAVAVGTASFWN
                     PEAVADVGRGIGEFLVQRGLRSPAGLRARVVVQEEEAGEPAHRGG"
     gene            complement(1345..2241)
                     /locus_tag="VF097_11300"
     CDS             complement(1345..2241)
                     /locus_tag="VF097_11300"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEX6263420.1"
                     /translation="MIRKKCEVLSSRRAGVYHTLTLVAPEIAEVARPGQFVQIAVPEG
                     REFLLRRPFSIHEASRRGGWAGTLELAFLGTGPGTTWLTQVQPHDFLDVIGPLGKPFP
                     YPRQLTNCLLVGGGYGAAPLYFLAKELNARQKRVDMIIGARDQERIFKQVEAKRLSVS
                     IGITTEDGSLGDRGRVTDILPGMVSRTGTEVVYACGPNAMLRAVAEYCADHGIPCQVA
                     VEELMACGMGICWTCTVPVIRRDGSGWDNLRTCVEGPVFNGARVWWEKWLREPSVPTQ
                     TPPHGFRMEEAGVGGEPWPEHE"
     gene            complement(2318..3619)
                     /locus_tag="VF097_11305"
     CDS             complement(2318..3619)
                     /locus_tag="VF097_11305"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006350351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX6263421.1"
                     /translation="MAKVLFRGGRVVDPASGTDRTADVLVEDTMISEVGEGLSPSGAA
                     EVVDCDGLVVAPGLVDLHTHLREPGREDAETIETGSRAAALGGYTAVVAMPNTDPVAD
                     QLAIVLEVAARGEKAGLVDVIPASAITKGLEGESLVEMGELSAAGVRLFTDDHRSVQS
                     ARTMRLALEYAKAFDVVICQHAQDDTLAEDAQMHEGHYSAILGLRGFPREGEEVTVAR
                     DILLARLSGGRLHVTHTSSAGSVELIRRARAEGVRVTADATPHHLALTDEALVTYDTR
                     FKVNPPIRSEEDRDGLRQGLADGTIDAVATDHAPHAVEDKEVEFDHARPGTTGLETAL
                     AVVLTELVEPGLLDLPSAIRAMSTEPARILGLHEQGGPFEPGQTANLVVFDPAEAWTV
                     GERPFATKGRNCAFVEREVRGRVRHTMLRGTFTVREGQATR"
     gene            complement(3619..4548)
                     /locus_tag="VF097_11310"
     CDS             complement(3619..4548)
                     /locus_tag="VF097_11310"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014446324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX6263422.1"
                     /translation="MRDLLSITQLSAEDITRILDTADSFREVGTRVIKKVPALRGRTV
                     VNLFYENSTRTRISFELAAKRLSADVINFSTSGSSVSKGESLKDTALTLQAMGADAIV
                     IRHSSSGAPYTLSRWVDASVLNAGDGTHEHPTQALLDLYTIREHFPSFEGLKVAIVGD
                     VRHSRVARSGVLALTKMGADVTLVGPPTLIPPEAPSWGAEVSYELDPVLPKLDVCYML
                     RVQRERQRQQFFPTVREYSQRYGLSEERVDALPDEALIMHPGPMNRGVEIASDVADLP
                     RSLIVDQVTNGIAVRMALLYLIVGVEGEQKEAS"
     gene            complement(4548..5093)
                     /gene="pyrR"
                     /locus_tag="VF097_11315"
     CDS             complement(4548..5093)
                     /gene="pyrR"
                     /locus_tag="VF097_11315"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX6263423.1"
                     /translation="MAFAASGVVLDAEGVRRALTRISHEILERNGGATDVVLVGIAAR
                     GDDLARRIAEKLRRIEDVDVPVGVLDISFYRDDIGLRAEAPEVHETRIDFDVTGRTVV
                     LVDDVLFTGRTIRAAMDALVDFGRPRAIQLAVLVDRGHRELPIRADFVGKNIPTRTGD
                     QVRVLTSETDGADAVEVGEET"
     gene            5269..5502
                     /gene="tatA"
                     /locus_tag="VF097_11320"
     CDS             5269..5502
                     /gene="tatA"
                     /locus_tag="VF097_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006340664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX6263424.1"
                     /translation="MDAVAVWTPGLGELIIILVVVLLLFGAKRLPEMARSLGRSTKEF
                     KKGMDDEAEEAEEDERREVDESRPAGDPPTSSR"
     gene            5543..6040
                     /locus_tag="VF097_11325"
     CDS             5543..6040
                     /locus_tag="VF097_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263425.1"
                     /translation="MRKVIVLVVATAALAAGVGGASAQPIDCGSFARIQTYRGWGPSL
                     HYGRAVTVVYGVEECSASWTDDTFEYSVTGSATIHDGVLPEGRHPGLLPVIESRVFTT
                     EGSFVDPDRTGWPPAWWSCLTSAEILWRIDGIYSFRAGATSGDWTLAISVLGAKPFRW
                     AYSAC"
     gene            complement(6025..6438)
                     /gene="nusB"
                     /locus_tag="VF097_11330"
     CDS             complement(6025..6438)
                     /gene="nusB"
                     /locus_tag="VF097_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX6263426.1"
                     /translation="MGARREARRRAALILYQADVTGRAPSEVLEERLELGEALPAFTT
                     ELVLGVGERLAELDEVMGRAAEEWTVERMASVDRTLLRLATFEILHRDDVPAAVAIDE
                     AVGAAKELSTEDSGRFVNGVLGRIARDAGAPQQAE"
     gene            complement(6440..7003)
                     /gene="efp"
                     /locus_tag="VF097_11335"
     CDS             complement(6440..7003)
                     /gene="efp"
                     /locus_tag="VF097_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007433861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX6263427.1"
                     /translation="MNVSTNDLKTGMTLDLDHQLWQVVDFQHVKPGKGHAFVRTKLKN
                     VRSGAVLDRTFRADEKVGLAIVERKEMQYLYDDGHALVFMDTGTYEQIHVPKDTVGDM
                     TGYLVEGAMALIAMHDGVPIGVELPAAVVIEVAQTDPGVKGDRVSGAMKPAVLQTGLT
                     VRVPLFVGPGDKVKVDTRSGEYLTREQ"
     gene            complement(7000..8067)
                     /locus_tag="VF097_11340"
     CDS             complement(7000..8067)
                     /locus_tag="VF097_11340"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019047839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEX6263428.1"
                     /translation="MDHATRRRRLAARLDDLGVEAILITRVVNVRYLSGFTGSNAQLL
                     LSADPVLLTDPRYDEQSSKEAPDLARRIYADGFRDELRPVLKAAGVARLGFERHAVTY
                     ESYESLAELEGVELVPIGHEVERLRWEKEPEEIALLERAQEVTDAAFDEIVGKLAEGM
                     TEREVAFELELAMRRGGAERIGFDTIAAFGENAAEPHHGPTDRALAPGDLVKLDFGCV
                     VDGYHSDMTRTLAFGDPGDELRKVYEIVARSQRAGEEAVRAGVVGGEADGAARRVIEE
                     AGHGDRFGHSLGHGVGLEIHEGPTLRRRSEDVLPAGAVVTVEPGIYLPGVGGVRIEDA
                     VVVEEDGCRALPRTTKELIVL"
     gene            complement(8016..8486)
                     /locus_tag="VF097_11345"
     CDS             complement(8016..8486)
                     /locus_tag="VF097_11345"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF003807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX6263429.1"
                     /translation="MNVLLLLGPSLGALGSRDPELYGDEPMEDVMAGVSARAEELGHR
                     LIWRQSDAEGELVGWLLAAREEGFEAVIANPGSLTHYSHALRDAVEASGLPAIEVHQT
                     NIFAREGFRRRSVISEACRAVIVGLGIGGYHVALEALGWIMQRGGDGSPRGSTT"
     gene            complement(8483..9562)
                     /gene="aroB"
                     /locus_tag="VF097_11350"
     CDS             complement(8483..9562)
                     /gene="aroB"
                     /locus_tag="VF097_11350"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX6263430.1"
                     /translation="MTTVPVRVRGAAYDVAIGVGLLARAGEVLPSFTGAERAFVVAAQ
                     PVAERFLGTLGASLELPAVHLAVPEGEEAKTLRTAEALWEHLATQGAHRADPVMALGG
                     GSTGDVAGFVAATYMRGVPFVQLPTTLTAQVDAAIGGKVGVNLPRGKNLVGAFHQPAA
                     VLADVSTLATLPEEEFRSGLGEAAKYGLAVDPWVLDVLERRHGAVLARDPGALADLVA
                     ACVRAKASVVEADERDTGARSVLNYGHTLAHALERLEGYGRVSHGEAVAMGMAFAARL
                     SVTLGIGSEDLPARHRRVLASLGLPKSFPELDPDEVLEAMHLDKKYRQGIRFVLLEEV
                     GRPRLVEVRPEAIRESLSEALGGVA"
     gene            complement(9564..10043)
                     /locus_tag="VF097_11355"
     CDS             complement(9564..10043)
                     /locus_tag="VF097_11355"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX6263431.1"
                     /translation="MKVWLVGMPASGKTTTGTLLARRLGVPFVDVDLEIESRAGRDVA
                     GIFAEAGEAGFRSLEREAIALLAEDGDAVIAAGGGAVLDPANREAMRASGRVVLLEAP
                     AEMLVARLGDAPRPVTAGADLEALLRDRAEAYAAAAHLRVEADGPPEAIVDRIVQGL"
     gene            complement(10040..11197)
                     /gene="aroC"
                     /locus_tag="VF097_11360"
     CDS             complement(10040..11197)
                     /gene="aroC"
                     /locus_tag="VF097_11360"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019434760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX6263432.1"
                     /translation="MRLLTAGESHGKALVAVLEGLPAGVPISEKEIEGELARRRHGYG
                     RSRRQRLEADRFEVLGGMRHGRTLGSPIAIRIENAEWEAKWSRRMAPFGSPDPDDRLV
                     RPRPGHADLTGAQKYGFDDVRNVLERASARETVARVAAGAFCKAFLAELGMAVLSHVV
                     RIGGEEGEPRTVPGPQDLPVVDASPVRCLDPEASELMVAAIDAARREGDSLGGVFEVV
                     AHGAPPGLGTYVHHERKLDARLALALMSIQAIKGVEVGDGFAGAARPGSRAHDEIEPS
                     GAGPRRRTGRSGGIEGGMSTGDPIRVRAAMKPLSTLPRPLDTVDLDTGEPAQAIRQRT
                     DVCAVPAAAVVGEAAVAHVLAEAALEKLGGDSVEETRRNLDGYLRSLEGGA"
BASE COUNT         1594 a         4415 c         3828 g         1605 t
ORIGIN      
        1 ggcctcgctc gcctcgaagg cgagcgaggc cggcgccgcc cccgccgcct ccgccaggct
       61 ggacagaacc gtgaccgcga tcacctcggg cggctccatc ccctcctcgg ccgctccctc
      121 ggcggccccg cgcacggccc cggcgaccat ctcggggcca cccagcgcgt gcacggtcag
      181 catggctgcg ccgagccgcc ccacgacacg ggaggcccgc tccacggtcg tggggatgtc
      241 gtgcagcttc aggtcgagga agacggggag gtgcccccgg aggcggcgga cggcctcggg
      301 accctccgac gcgaacagct cgaggcccac cttgaccatg cccacggtcc cggccagcga
      361 gcgggccaga tcctcggcgg cggcgaccga tgggacgtca gccgccacga tgagccggct
      421 caccggcctc ctcttcctgc accaccactc gagcccggag gccggccggg ctcctcagcc
      481 cgcgctggac caggaactcc ccgatcccgc ggcccacgtc ggccaccgcc tcggggttcc
      541 agaagctcgc cgtccccacc gccacggccc aggcgcccgc cagcaggaac tcgaccgcgt
      601 cccgggcgct gcggatccct cccatcccga tgatgggaac ctcggggagc gcccgcgcca
      661 cctggtagac ggcgcgcacc gccaccggcc ggatcgcggg gcccgacagc ccgcccgtca
      721 ccccaccaag cttggggcgg aaggtctcga cgtcgatcgc catcgccaaa agcgtgttga
      781 tgagggagat gccgtgggcg ccggcccgga cgcaggcctc ggccacctcg gcgatgtcgg
      841 tgacgtcggg tgtcagcttg gcgaagaccg gcagccgcga gagcctggcc accgcgccca
      901 cgacctcggc cgccgcgtcc gggtggcagg cgaaaacctg attgcgccgc tcgacgttgg
      961 gacaggagat gttcgcctcc agcgccacga ccccggggat gttctgggcg cgcatcgcca
     1021 cctgaacgaa ctcctcgacc gacttccccg cgatcgagag gaagaccggc acgcccaccc
     1081 gccggatgaa cggtccctcc ttctccatga actcgtcgat gcccgggttc tggaggccga
     1141 tcgcgttcag catgcccgac ggggtctcga ccatgcgggg ggtgggcagc cccttcaccg
     1201 gccggagggt cacgctcttc gtgacgatcc ctccgacctt ggagaggtcg aggatcggag
     1261 agagctcacg gccggacccg aacgtcccgg aggccccgag cacgggggtg gggagctgca
     1321 cgcccccaag gtcgaccccg agattcactc gtgctcgggc cacggctcac ctcccacccc
     1381 ggcctcctcc atgcggaacc cgtggggagg ggtctgcgtg gggaccgagg gctcccggag
     1441 ccacttctcc caccagacgc gcgcgccgtt gaagaccggg ccctccacgc acgtacggag
     1501 gttgtcccag ccggacccgt ctcggcggat caccgggact gtgcaggtcc agcagatgcc
     1561 catgccgcac gccatcagct cctccacggc cacctggcac gggatcccgt ggtcggcgca
     1621 gtactccgcc actgcccgga gcatcgcgtt cgggccgcac gcgtacacga cctccgtgcc
     1681 cgtccgcgac accatcccgg ggaggatgtc agtgacgcgc ccccggtcgc cgaggctgcc
     1741 gtcctcggtc gtgatcccga tcgacaccga gagacgcttc gcctcgacct gcttgaagat
     1801 gcgctcctgg tcgcgggccc cgatgatcat gtccacacgc ttttgacgtg cgttcagctc
     1861 cttcgccagg aagtagagag gggccgcgcc gtagccgccg ccgaccagga ggcagttcgt
     1921 cagttgtcgc gggtacggaa agggcttccc gagcggcccg atcacgtcga ggaaatcgtg
     1981 cggctggacc tgcgtcagcc acgtggtgcc cggacccgtc cccaggaagg cgagctccag
     2041 ggtgccggcc cagcctccac gcctggacgc ctcgtgaatc gagaagggac ggcgcagcag
     2101 gaactcgcgg ccctcgggga cggcgatctg cacgaactgg ccggggcgtg ccacctcggc
     2161 gatctccggc gccaccaggg tcagcgtgtg gtacacgccc gcacgccgcg agctgaggac
     2221 ctcgcacttc ttgcggatca cggcaccgag cctactcccc gtcgtgatgc cgggcaccac
     2281 cgctgccgag gcgttccgtc cgccggcggg gcgtcgctca cctcgtggct tggccctccc
     2341 ggacggtgaa cgtcccccgg agcatcgtgt ggcggacccg accccgaacc tcccgctcca
     2401 cgaacgcgca gttgcgcccc tttgtggcga agggccgctc ccccacggtc caggcctcgg
     2461 ccgggtcgaa gacgacgagg ttcgccgtct gccccggctc gaagggcccg ccctgctcgt
     2521 ggaggcccag gatccgcgcc ggctccgtgg acatcgcccg gatcgcgctc ggcaggtcga
     2581 gcaggccggg ctccacgagc tcggtcagca cgaccgccag agccgtctcc aggccggtcg
     2641 tcccggggcg agcgtggtcg aactcgacct ccttgtcctc cacggcgtgg ggggcgtgat
     2701 cggtggccac ggcgtcgatc gtcccgtcgg ccagcccctg ccggagaccg tcgcgatcct
     2761 cctcggaccg gatcgggggg ttgaccttga acctcgtgtc gtacgtgacg agagcctcgt
     2821 cggtgagggc gaggtggtgg ggtgtggcgt ccgccgtgac ccgcacaccc tcggctcggg
     2881 ccctcctgat cagctcgacc gatcccgccg acgaggtgtg ggtgacgtgg agccggcccc
     2941 cgctgaggcg agcgaggagg atgtcgcggg ccaccgtgac ctcctcgccc tcgcggggga
     3001 agcctcggag gccgaggatc gccgagtagt ggccctcgtg catctgcgcg tcctcggcca
     3061 aggtgtcgtc ctgcgcgtgc tggcagatga ccacgtcgaa ggccttcgcg tactccagcg
     3121 cgagccgcat cgtcctggcc gactgcaccg accggtggtc gtcggtgaaa agccggacgc
     3181 cggccgcgga aagctccccc atctcgacga gggactcccc ctcgagcccc ttcgtgatcg
     3241 ccgatgccgg gatcacgtcc accaggcccg ctttctcgcc ccgggccgcg acctctagca
     3301 cgatcgcgag ctggtcggcg accgggtcgg tgttcggcat cgccacgacc gccgtgtagc
     3361 cgcccagcgc ggccgccctg ctgcccgtct cgatcgtctc cgcatcctcc cggccgggct
     3421 cccgaagatg cgtgtggagg tccacgagcc ccggcgcgac cacgaggcca tcgcagtcca
     3481 cgacctcggc cgcgcccgac ggggacagcc cttcccccac ctccgagatc atggtgtcct
     3541 cgacgaggac gtcggccgtg cgatccgtcc cggaggcggg atcgacgacc ctccctccgc
     3601 ggaagagaac cttcgccatc aactcgcctc cttctgctca ccctccaccc cgacgatcag
     3661 atagagaagg gccatccgca ccgcgatccc gttcgtgacc tgatccacga tcaacgagcg
     3721 ggggaggtcg gccacgtccg aggcgatctc gaccccgcgg ttcatcgggc ccgggtgcat
     3781 gatcaacgcc tcgtcgggca gggcgtccac ccgctcctcc gacagcccgt accgctggga
     3841 gtactcgcgc accgtgggga agaactgctg gcgctgccgc tcccgctgga ccctcagcat
     3901 gtagcagacg tcgagcttgg gcaggaccgg gtcgagctcg taggagacct cggcgcccca
     3961 ggatggggcc tcgggcggga tcagcgtggg cgggcccacg agcgtcacgt ccgcccccat
     4021 cttcgtcagg gcgagcacgc ccgagcgagc cacccgggag tgtcggacgt cgcccacgat
     4081 cgctaccttc agcccctcga acgacgggaa gtgctcgcgg atcgtgtaga ggtcgaggag
     4141 agcctgggtg ggatgctcat gggtcccgtc gcccgcgttg agaacgctcg cgtccaccca
     4201 gcggctcagc gtgtagggcg cgccggacga cgagtgccgg atcacgatcg cgtcggcgcc
     4261 catcgcctgg agggtcagcg ccgtgtcctt caggctctcg cccttcgaga cggaggatcc
     4321 cgaggtggag aagttgatca cgtccgcgga gagccgcttc gcggcgagct cgaacgagat
     4381 gcgggtccgg gtggagttct cgtagaagag gttgacgacg gtgcgcccgc gcagggccgg
     4441 caccttcttg atcacacggg tccccacctc gcggaacgag tcggccgtgt cgaggatcct
     4501 cgtgatgtcc tcggccgaga gctgcgtgat cgacaagagg tccctgatca tgtctcctcc
     4561 cccacctcga cggcgtcggc gccgtcggtt tcggacgtca ggacccggac ctggtccccc
     4621 gtccgggtcg ggatgttctt gcccacgaag tcggcgcgga tcgggagctc gcggtggccc
     4681 cgatccacga gcacggcgag ctggatggcg cggggtcgtc cgaagtccac gagggcgtcc
     4741 atcgcggcgc ggatcgtccg gccggtgaac agcacgtcgt ccacgagcac gaccgtgcgc
     4801 ccggtcacgt cgaagtcgat gcgggtctcg tggacctccg gcgcctcagc ccggagcccg
     4861 atgtcgtcgc ggtagaagct gatgtcgagg acccccacgg ggacgtccac atcctcgatc
     4921 cgtctgagct tctcggcgat gcggcgggcc agatcgtcgc ccctggcggc gatccccacg
     4981 aggacgacgt cggtcgcccc cccgttccgc tccaggatct cgtgggagat gcgcgtcagg
     5041 gcgcggcgga cgccctcggc gtcgagcacg acgccgctag ccgcgaaggc cacgacccgc
     5101 tcccggacgc cagaacgccc caccgccgta cggcagggcg cccttcctct ccacgatgca
     5161 cgctccttcc cggcctcacg ggaccggact taaagggaag cttcctgcgc ggagcctacc
     5221 acagagatgg ccggtcgcat gacgggcggg ggggtgtatc ctggcctcat ggacgctgtc
     5281 gcggtgtgga cgccggggct cggcgagctc atcatcatcc tcgtcgtcgt cctcctgctc
     5341 ttcggcgcga agcgcctccc cgagatggct cggtcgctgg ggcgctccac caaggagttc
     5401 aagaagggca tggacgacga ggccgaagag gccgaggagg acgaacgccg agaggtcgac
     5461 gagagccggc cggccggcga cccgccgacg tcatccaggt agcgccttcc ggcgccgagc
     5521 gagcgagatc ccaggggggc acgtgaggaa ggtcatcgtc ctcgtcgtcg ccaccgcagc
     5581 cctggccgcc ggggtcggag gtgcgagcgc ccagcccatc gactgcggga gcttcgcccg
     5641 tatccagacg taccggggct gggggccctc cctccactac ggacgggccg tcaccgtcgt
     5701 ctacggcgta gaggagtgct cggcgagctg gaccgacgac accttcgagt actcggtgac
     5761 cgggtcggcc acgatccacg acggggtgct gcccgagggg aggcatcccg gcctgctccc
     5821 ggtcatcgag tcgagggtct tcaccaccga gggatccttc gtggatccgg accggaccgg
     5881 gtggccgccc gcgtggtggt cgtgcctgac ctccgccgag atcctctgga ggatcgacgg
     5941 catctactcg ttccgggccg gggccacgag cggcgactgg accctcgcca tcagcgtcct
     6001 gggcgcgaag cccttccgct gggcctactc ggcctgctga ggggcgccgg cgtcccgggc
     6061 gatcctcccc agcacgccgt tcacgaaccg ccccgagtcc tcggtggaga gctccttggc
     6121 cgcgcccacg gcctcgtcga tcgccacggc ggccggcacg tcgtcgcggt ggaggatctc
     6181 gaaggtcgcc agccgaagga gggttcggtc gacggacgcc atcctctcca cggtccactc
     6241 ctcggcggcc cgccccatca cctcgtcgag ctcggcgagg cgctccccaa cccccagcac
     6301 gagctccgtg gtgaacgcgg ggagcgcctc gccgagctcg agccgctcct cgaggacctc
     6361 ggagggcgcc ctccccgtga cgtcggcctg gtagaggatg agcgcggcgc ggcggcgagc
     6421 ctcgcgccgc gcccccacct cactgctccc gcgtgaggta ctcgcccgat cgcgtgtcga
     6481 ccttgacctt gtcgcccggc cccacgaaca gcggcacccg gaccgtgagg cccgtctgca
     6541 gcaccgccgg cttcatcgca ccgctcaccc ggtcgccctt cacgccggga tcggtctgcg
     6601 cgacctcgat cacgaccgcg gcgggcagct ccaccccgat cggcacgccg tcgtgcatcg
     6661 cgatcagggc catcgcgccc tcgacgaggt agccggtcat gtcccccacc gtgtccttcg
     6721 ggacgtggat ctgctcgtag gtccccgtgt ccatgaacac gagagcgtgc ccgtcgtcgt
     6781 agaggtactg catctccttc cgctccacga tcgccaggcc gaccttctcg tcggcccgga
     6841 acgtccggtc gagcaccgcg ccgctgcgga cgttcttcag cttcgtgcgc acgaaggcgt
     6901 gtcccttccc cggcttcacg tgctggaagt ccacgacctg ccagagttgg tggtcgaggt
     6961 cgagggtcat cccggtcttc aggtcgttcg tcgagacgtt cacagcacga tcagctcctt
     7021 ggtggtcctg gggagggccc ggcacccgtc ctcctcgacc accacggcgt cctcgatccg
     7081 aacgccgccc acgcccggca ggtagatgcc cggctccacg gtcaccacgg cgcccgcggg
     7141 gaggacgtcc tccgacctcc tccgcagggt cggcccctcg tggatctcca ggcccacccc
     7201 atggcccagc gagtgcccga agcggtcgcc gtggccggcc tcctcgatca cgcgacgggc
     7261 ggccccgtcg gcctccccgc cgacgacgcc ggcccggacc gcctcctcgc cggctcgctg
     7321 ggacctggcc acgatctcgt agaccttccg cagctcgtcg cccgggtccc cgaacgccag
     7381 cgtcctcgtc atgtccgagt ggtagccgtc caccacgcat ccgaaatcaa gcttgacgag
     7441 gtctcccggg gccagtgccc ggtcggtcgg gccgtgatgc ggctcggcag cgttctcgcc
     7501 gaaggcggcg atcgtgtcga acccgatccg ctcggccccg cctcgccgca tcgccagctc
     7561 gagctcgaac gccacctcgc gctcggtcat cccctcggcc agcttcccca cgatctcgtc
     7621 gaaggccgcg tcggtaacct cctgggcccg ctccaggagg gcgatctcct ccggctcctt
     7681 ctcccagcgg agccgctcca cctcgtggcc gatcgggacg agctccacgc cctcaagctc
     7741 ggccagcgac tcgtagctct cgtaggtgac ggcgtgacgc tcgaacccga ggcgcgcgac
     7801 gcccgccgcc ttcagcaccg gcctgagctc gtcgcggaac ccgtccgcgt agatgcggcg
     7861 ggcgaggtcg ggcgcctcct tcgaggactg ctcgtcgtag cgggggtcag tcaggaggac
     7921 ggggtcggcc gagagcagca gctgcgcgtt cgagccggtg aaccccgaga ggtagcggac
     7981 gttcaccact cgggtgatca ggatcgcctc gacccctagg tcgtcgagcc gcgcggcgag
     8041 ccgtcgcctc ctcgttgcat gatccatccc aaggcctcca gcgccacatg atagccgccg
     8101 atccccaggc ccacgatcac ggcccggcac gcctccgaga tcacggagcg gcgccggaac
     8161 ccctcgcggg cgaagatgtt ggtctggtgc acttcgatcg cgggcaggcc cgaggcctcc
     8221 accgcgtccc gcagggcgtg ggagtagtgg gtgagcgagc ctgggttcgc gatcaccgcc
     8281 tcgaatccct cctcccgtgc cgcgagcagc catcccacca gctccccctc ggcgtcgctc
     8341 tggcgccaga tcagccggtg gcccagctcc tcggcccggg ccgagacccc cgccatcacg
     8401 tcctccatcg gctcgtcccc gtacagctcc ggatcccggg agcccagggc gccgaggctc
     8461 ggtcccagga gcagcagcac gttcacgcca cgcctcccag cgcctcgctc agcgactcgc
     8521 ggatcgcctc cggccgcacc tccaccagcc ggggccgtcc cacttcctcg aggagcacga
     8581 agcggatgcc ctgtcggtac ttcttgtcga ggtgcatggc ctcgaggacc tcgtcggggt
     8641 cgagctcggg gaacgacttg gggaggccca gcgaggccag cacccgccgg tgcctcgccg
     8701 ggaggtcctc cgagccgatg ccgagcgtca ccgagagccg cgcggcgaag gccatcccca
     8761 tcgccaccgc ctccccgtgg gagacccgcc cgtatccctc gagccgctcc agggcgtggg
     8821 ccagtgtgtg gccgtagttg aggaccgacc gggcgccggt gtcccgctcg tcggcctcca
     8881 ccaccgacgc cttggcccgc acgcaggcgg ccacgaggtc ggcgagcgcc cccggatccc
     8941 gggccagcac cgcgccgtgc ctccgctcca gcacgtcgag gacccaggga tccacggcga
     9001 gcccgtactt ggccgcctcg cccaggcccg accgaaactc ctcctcgggc agcgtcgcca
     9061 gcgtggagac gtcggccagc acggccgcgg gctggtggaa ggcgcccacc aggttcttgc
     9121 cccgggggag gttgacgccg accttccccc cgatcgcggc atcgacctgg gccgtgaggg
     9181 tcgtggggag ctggacgaac ggcacgccgc gcatgtaggt ggccgcgacg aatcctgcca
     9241 cgtcgccggt cgagcctccg cccagcgcca tcactggatc cgcgcggtgc gccccctgcg
     9301 tcgccagatg ctcccagagc gcctcggccg ttcgcagggt cttcgcctcc tcgccctcgg
     9361 ggacggccag gtgcactgcc ggcagctcga gcgaggcccc gagcgtcccc aggaaccgct
     9421 cggccaccgg ctgggcggcc accacgaacg cccgctcggc gccggtgaag gacggcagga
     9481 cctcccccgc ccgggccagc agcccgacgc cgatcgcgac gtcgtaagcg gccccgcgca
     9541 cgcggacggg caccgtggtc acgtcagagc ccctggacga tgcggtcgac gatcgcctcc
     9601 ggcggcccgt cggcctccac cctgaggtgc gcggcggcgg cgtaggcctc ggcgcggtcg
     9661 cgcagcagcg cctcgagatc cgcgccggcg gtcaccgggc gcggcgcgtc ccccagcctg
     9721 gccaccagca tctcggccgg ggcctcgagc aggacgaccc ggcccgaggc ccgcatcgcc
     9781 tcgcggttcg ccgggtccag caccgcccca ccgcccgccg cgatcaccgc gtcgccgtcc
     9841 tccgccagca gggcgatcgc ctcccgctcc agggagcgga agcccgcctc acccgcctcg
     9901 gcgaagatcc ccgccacgtc gcggccggcc cgggactcga tctcgagatc cacgtccacg
     9961 aacgggaccc cgagacgccg ggcgaggagg gtccccgtcg tggtcttccc cgaggccggc
    10021 atcccgacga gccagacctt cacgcgccac cctccagcga gcggaggtac ccgtcgaggt
    10081 tgcggcgcgt ctcctcgacc gagtcgcccc ccagcttctc cagggcggcc tccgccagca
    10141 cgtgggccac cgcggcctca cccacgaccg cggcggcggg gaccgcgcac acgtcggtcc
    10201 gctgacggat cgcctgcgcc ggctcccccg tgtccaggtc cacggtgtcc aggggtcggg
    10261 gcagcgtcga gaggggcttc atcgccgccc gcacccggat cgggtcgccc gtggacatgc
    10321 cgccctcgat ccctcccgac cttccggtgc ggcggcgcgg cccggctccg ctcggctcga
    10381 tctcatcgtg ggcccgggag ccgggccgcg ccgcccccgc gaacccgtcc cccacctcca
    10441 cgcccttgat cgcctggatg ctcatgagcg ccagcgcgag ccgcgcgtcc agcttccgct
    10501 cgtggtgcac gtaggtcccg agccccggcg gcgccccgtg cgccacgacc tcgaacacgc
    10561 cgccgagact gtccccttcg cgtcgggccg cgtcgatcgc cgccaccatc aactccgagg
    10621 cctcggggtc gaggcaccga accggcgacg cgtcgacgac gggaaggtcc tggggcccgg
    10681 gaacggtcct gggctcgccc tcctcacctc cgatccgcac cacgtgcgag agcacggcca
    10741 tccccagctc ggcgaggaag gccttgcaga acgctccggc cgccactcgg gccacggtct
    10801 cgcgcgcgct ggcccgctcc agcacgttgc ggacgtcgtc gaagccgtac ttctgcgccc
    10861 cggtcaggtc cgcgtggccg ggacgtggac ggaccagccg gtcgtcggga tccggggagc
    10921 cgaacggggc catccggcgg ctccacttcg cctcccactc cgcgttctcg atccggatcg
    10981 cgatcgggct gcccagggtt cgcccgtgcc gcatcccccc gagcacctcg aagcgatcgg
    11041 cctccagccg ctgacgccgg ctccgcccgt agccgtggcg cctccgggcc agctcgccct
    11101 cgatctcctt ctcggagatg gggacgcccg ccggcaggcc ctccagcacg gccacgaggg
    11161 ccttcccgtg cgattcgccc gcggtcagca gtctcatcgt gccagcatac ggaggaggcg
    11221 gcccgaaggc cgcctcccag ctccgacggc cgctcccccc gcggtcgcag ccatcggacc
    11281 ccgccggccg ggcgggtcag tcccggccgc gtcccatcgg gccgcgcggg ggcgtcactt
    11341 cctctctccc ggctcgcaga gcgtgaagga ctggtcgccg aaccggaagt tggcgcaccc
    11401 tccctgcacc gagacgagct ggaagttgtc ggcgaacgtc tc
//
LOCUS       DASUTZ010000265         6008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1269670, whole genome shotgun sequence.
ACCESSION   DASUTZ010000265 DASUTZ010000000
VERSION     DASUTZ010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269670"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VF097_11365"
     CDS             complement(<1..225)
                     /locus_tag="VF097_11365"
                     /inference="COORDINATES: protein motif:HMM:NF015042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX6263433.1"
                     /translation="MASVLVAMSGGVDSSVAACLLHEAGHEVLGSHMKLVHTEGVEHG
                     CCGPQAEADARAVAEHAGFAFEVADLSEVFE"
     gene            complement(264..506)
                     /locus_tag="VF097_11370"
     CDS             complement(264..506)
                     /locus_tag="VF097_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263434.1"
                     /translation="MSGRDEDRHVIDPSKGLVAEGVPERKAWEVRRRGTLAGIVWREG
                     RASFRAAYERDGRMTQIPRTFLSIEEAARALDRAVR"
     gene            560..1201
                     /locus_tag="VF097_11375"
     CDS             560..1201
                     /locus_tag="VF097_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017621179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX6263435.1"
                     /translation="MLIDLSHPIEEGRGAFPGLPPPRIGPILTHEDSRDRYQGKAEFF
                     LGHAEIATNTGTYLDSPFHRFRDREDIGGIPLEKVANLPGLVVDASPDPGPVELPEGD
                     LERRAVLIRTGRDELWPTDEYWARGPFLGEEAISALIEGGAALVGVDFGNVDDVEDSS
                     RPAHTRLLEAGVLIVENLTGLGPLPRERFRFTAAPPAVVGAASFPVRAFAEIP"
     gene            complement(1198..2130)
                     /locus_tag="VF097_11380"
     CDS             complement(1198..2130)
                     /locus_tag="VF097_11380"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX6263436.1"
                     /translation="MSDATPSSFVELVGCRLPLQLAAMGGIGTPELAASVSRAGGLGT
                     VPGRRPDDVERSVEEMRASGEGPVAISFLMPFLRRESVEVAARLADVVEFFYGEPDRE
                     LIEVAGRGGAVVGWQVGTAREATAARDAGCRLIAVQGVEAGGHLRGSQPLDVVLEQTL
                     RVVDVPVLAAGGIGTSERVEELFDMGAVGVRIGTRFIAAAECQAHPAYVGALIGATAR
                     DTVVTEAFGADWPDAPHRVLRSALRALERFEGEVVGSIGPWQMPRASSVPPTRETEGV
                     IEAMALYAGESVEAVHGVVPAAQIVEELVGSRSA"
     gene            complement(2127..3587)
                     /locus_tag="VF097_11385"
     CDS             complement(2127..3587)
                     /locus_tag="VF097_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263437.1"
                     /translation="MARPWDRLSSRDQARLQSHLLRELLTRYVYPFSPFYRRLLDQRG
                     IKPGDVKRLRDIERLPMVSRWDFAATDEDRWRPFRAMLRPDERSLKRWAHRRTLRRVA
                     IEKLLRGDLAAERMVAEEFKPIHLHLPERAGPVMGYTMRDLSGLSQAGARSLAVIGAD
                     RSDVLVSTLPSGPTLPFWHVRYAAQGAGIAALHLGAGESVRPARAAHWMHRAGATVLV
                     SQPGYAEGLLRGAPPETFERLRILALWSEGGMQGARERFNSRLRAGGAPEAYVVSLLG
                     IPEARVAWAECPPPADRPGASHGFHTYPDLEYLEVVDPSTGKLQDEERGGELVYTSLD
                     WRGSALLRYRTGVVARRGITTKPCPGCGRTVPRILPEISYTDWLVRTTGSQGEVRLDL
                     AEVLPVLWEARGVPLWQVEVGRGAGPGRLDLVTACLGGAGERDAEQVERALRPFGVRC
                     RPVPLQDLGRRMGMGTERPEERVVVRRDPSSSGPGS"
     gene            complement(3592..4572)
                     /locus_tag="VF097_11390"
     CDS             complement(3592..4572)
                     /locus_tag="VF097_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX6263438.1"
                     /translation="MAEIWTVVDHSGGEPSKVSLQMVTAGRKLAEQMGHDLAGIFLGT
                     GWETAKEKLAKHALPKAYVFESQDFEDYLVQPAIDAIVKLAQEHQPAAILFASVPLGK
                     DLASGVAAKLESGGLADVTDIEVDGDRLTCRMDVFGGATITKCQVVGDPQVICVKPNA
                     FVAQEAAADLAEERIEAEITDDAKAARIVERVQEEAGGRPSVEEAAIIVSGGRGLGGP
                     EHFSLVEELADVLGAGVGASRAAVDAGWYPHQNQVGQTGKTVSPQLYIAVGISGAIQH
                     RAGMQTSKTIVAINKDAEAPIFNLSDFGVVGDLFEVLPKLTEELKKRKGG"
     gene            complement(4572..5360)
                     /locus_tag="VF097_11395"
     CDS             complement(4572..5360)
                     /locus_tag="VF097_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005458414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX6263439.1"
                     /translation="MLNIVVGVKRVPDTAAEKKLDEQDFTLDRESVEAIMNPVDEYAI
                     EEAIKLRDAAGEGEVTVLTMGPEPADKEAVRKALALGADKGILVTDDRLHGSDAMATA
                     YVLALALAQQDFDLMLLGSESTDARTSLVPQALGQLMGMTALTYVKQIEVSDGKVTVH
                     REEEGGHAVVEAELPAIVSVVKGINEPRYATLKGIMAAKQKEVQVLAGDELGIDPEQV
                     GLEGSKTKVLEASPRPPKEKGEVVKDEGDAHEKLADFLQANKFI"
     gene            5476..>6008
                     /locus_tag="VF097_11400"
     CDS             5476..>6008
                     /locus_tag="VF097_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263440.1"
                     /translation="MRRFRLEAVLVVLLVAALALTFVLRGAPVGTGGDDDRETADPMG
                     GATPCIQALFGEPVPGPPAAQADVASVAPVVEELRELTFEEEPAPVYLDPPALSERVT
                     EMVEEEDEFLAIQARLLTEIGALPEGIDLDAEIQDLLGEQVGGFYDSDTGELVVADDA
                     REGLTTVELLTLVHELQ"
BASE COUNT          830 a         2290 c         2029 g          859 t
ORIGIN      
        1 ctcgaagacc tcggagaggt cggccacctc gaacgcgaac ccggcgtgct ccgcgacggc
       61 gcgagcgtcg gcctcggcct ggggcccgca gcacccgtgc tccacaccct cggtgtggac
      121 gagcttcatg tgggagccca gcacctcgtg gcccgcctcg tggaggagac aggcggcgac
      181 cgaggagtcc acgcccccgg acatcgcgac gagcacgctg gccatcgagg tcgagggtag
      241 caccgcggcc ccgccggccc gcgtcagcgg acggcgcggt cgagggcccg ggcggcctcc
      301 tcgatcgaga ggaacgtccg ggggatctgg gtcatgcggc cgtcgcgctc gtacgcggcg
      361 cggaacgaag ccctcccctc cctccagacg atgccggcca gcgtcccccg ccgccgcacc
      421 tcccaggcct tccgctcggg cacgccctcg gccacgagtc ctttcgaggg gtcgatcacg
      481 tggcgatcct cgtcgcggcc cgacatgaga gaggtatacc cgaacgggcg tccccacacg
      541 aacccggtag catcgccgca tgctgatcga cctgagccat ccgatcgagg aggggcgcgg
      601 ggcgttcccg gggctgcctc ctccccgcat cggccccatc ctgacgcacg aggactcccg
      661 ggaccgctat caagggaagg ccgagttctt cctggggcac gcggagatcg ccacgaacac
      721 ggggacctac ctggactcgc cgttccaccg gttccgcgat cgcgaggaca tcggagggat
      781 cccgctggag aaggtcgcga acctcccggg gctcgtcgtg gacgcgtcgc ccgacccggg
      841 cccggtggag ctcccggagg gagacctcga gcgccgcgcc gtcctgatcc gcaccggccg
      901 cgatgagctg tggccgaccg acgagtactg ggcccggggc ccgttcctgg gcgaggaggc
      961 gatctcggcg ctgatcgagg gcggcgccgc gctcgtgggg gtcgactttg gcaacgtgga
     1021 cgacgtcgag gattcgtcga ggcccgcgca cacgcgcctg ctggaagcgg gcgtcctcat
     1081 cgtggagaac ctgacgggcc tggggccgct gccgcgcgag aggttccggt tcacggccgc
     1141 gcccccggcg gtcgtcggcg ccgcctcctt cccggtgcgc gcgttcgccg agatccccta
     1201 ggccgacctc gatcccacca gctcctccac gatctgcgcc gcggggacga cgccgtggac
     1261 ggcctccacc gattcgccgg cgtacagcgc catcgcttcg atcacgcctt ctgtctctcg
     1321 cgttggaggg accgagctcg ctcgcggcat ctgccagggg ccgatcgagc cgacgacctc
     1381 cccctcgaac cgctccaggg ccctcagggc cgaacgaagg acccggtgag gcgcgtcggg
     1441 ccagtcggcg ccgaacgcct ccgtcacgac ggtgtccctc gccgtcgccc cgatgagggc
     1501 tccgacgtag gccggatgcg cctgacactc cgccgcggcg atgaaccgcg tcccgatccg
     1561 gacaccgact gcgcccatgt cgaacagctc ctcgacgcgc tcggacgtgc cgatcccgcc
     1621 cgccgcgagc accggcacat cgacgaccct aagggtctgc tcgaggacca cgtcgagagg
     1681 ctgagacccg cggaggtgcc cacccgcttc gacgccctgg acggcgatga ggcggcagcc
     1741 cgcgtcgcgg gccgcggtgg cttcccgggc cgtccccacc tgccagccga cgacggcgcc
     1801 accgcggccc gcgacctcga tgagctctcg atccggttct ccgtagaaga attccacgac
     1861 gtccgcgagc ctcgccgcca cctccacgga ctcacgacga aggaagggca tcaggaagct
     1921 gatcgcgacg ggcccttccc cggaggccct catctcctcg accgaccgct cgacgtcgtc
     1981 gggacgacgg ccgggcactg tgcccagacc gccggcacgg gagacggagg ccgccagctc
     2041 cggggtgccg atccctccca tcgcggcgag ctgaaggggg agacggcagc ccaccagctc
     2101 cacgaacgaa gatggtgtgg cgtcgctcac gacccgggcc cactcgacga ggggtcgcgg
     2161 cggaccacca cccgctcctc ggggcgctcg gtccccatcc ccatccgccg tccgaggtcc
     2221 tggagcggca ccgggcggca ccgaacgccg aacggcctca gcgcccgctc cacctgctct
     2281 gcgtcgcgct cgcccgcccc gcccagacac gcggtcacca ggtcgagccg gccgggtccg
     2341 gcgccccggc ccacctccac ctgccacagc ggcacccccc gggcctccca cagcacgggc
     2401 aggacctcgg ccagatccag ccgcacctcg ccctgggagc cggtcgttcg caccagccag
     2461 tccgtgtagg agatctccgg gaggatcctg ggcaccgtcc gcccgcatcc cgggcagggc
     2521 ttcgtcgtga tcccccgccg ggccacgacg ccggtccggt accggaggag ggccgacccg
     2581 cgccagtcga gcgaggtgta cacgagctcg ccgccgcgct cctcgtcctg gagcttcccc
     2641 gtcgagggat ccacgacctc caggtactcg agatcggggt aggtgtggaa cccgtgcgag
     2701 gcgcccggtc gatccgcggg gggcgggcac tcggcccagg ccacccgggc ctcggggatc
     2761 cccagcaggg agacgacgta ggcctcgggc gccccgccag cgcgcagcct cgagttgaac
     2821 cgctcgcgcg ctccctgcat ccctccctcg ctccacaacg cgaggatgcg caggcgctcg
     2881 aacgtctcgg gcggggcccc tcgaagcagg ccctccgcgt accccggctg agagaccagc
     2941 accgtggccc ccgcgcggtg catccagtgc gccgcccgtg ccggccgcac ggactccccc
     3001 gcccccaggt gaagggcggc tatgccggcg ccctgggccg cgtagcgcac gtgccagaac
     3061 gggagcgtcg gtccggaggg gagggtcgag acgaggacgt ccgaccggtc cgcgccgatg
     3121 accgcgaggc tcctcgcgcc cgcctgcgag agccccgaga ggtcgcgcat cgtgtagccc
     3181 atcaccggcc ccgcccgctc gggcaggtgg aggtggatcg gcttgaactc ctcggccacc
     3241 atgcgctcgg ccgccaggtc gccgcgcagc agcttctcga tggcgacccg gcggagggtc
     3301 cgccggtgcg cccagcgctt caggctgcgc tcgtcggggc ggagcatcgc ccggaatggc
     3361 cgccaccggt cctcgtcggt ggccgcgaag tcccagcggg agaccatcgg gaggcgctcg
     3421 atgtcgcgga gccgcttcac gtcgcccggc ttgatccccc gctgatcgag cagccgccgg
     3481 tagaacgggc tgaacgggta gacgtagcgc gttaggagct cgcgcaggag gtgcgactgg
     3541 aggcgagcct ggtccctgga agagaggcgg tcccagggac gggccaccgc ctcacccgcc
     3601 cttgcgcttc ttcagctcct ccgtgagctt cgggaggacc tcgaagaggt cgcccacgac
     3661 gccgaagtcg gagaggttga agatcggggc ctccgcgtcc ttgttgatgg cgacgatcgt
     3721 cttggaggtc tgcatccccg cgcggtgctg gatcgcgccc gagatcccca cggcgatgta
     3781 cagctggggc gagacggtct tcccggtctg ccccacctgg ttctgatggg ggtaccagcc
     3841 ggcgtccacc gccgcccgcg aggcccccac gccggcgccc agcacgtcgg ccagctcctc
     3901 cacgagcgag aagtgctccg ggcccccgag gccccgccca cccgagacga tgatggcggc
     3961 ctcctccacg ctgggccttc ccccggcctc ctcctggacc cgctccacga tcctcgcggc
     4021 cttggcgtcg tccgtgatct cggcctcgat ccgctcctcg gccaggtcgg cggccgcctc
     4081 ctgggccacg aacgcgttcg gcttcacgca gatcacctgc gggtccccca cgacctggca
     4141 cttcgtgatc gtggccccgc cgaagacgtc catgcggcac gtgagccggt cgccgtcgac
     4201 ctcgatgtcg gtgacgtcgg ccagcccccc gctctccagc ttggccgcga cgcccgaggc
     4261 caggtccttc cccagcggga cgctggcgaa caggatcgcc gcgggctggt gctcctgcgc
     4321 cagcttcacg atcgcgtcga tcgccggctg aacgaggtag tcctcgaagt cctgcgactc
     4381 gaagacgtag gccttgggga gcgcgtgctt ggccagcttc tcctttgccg tctcccagcc
     4441 ggtgccgagg aagatcccgg ccaggtcgtg ccccatctgc tccgcgagct tgcgccctgc
     4501 ggtgaccatc tggagggaga ccttgctcgg ctcccctccg ctgtggtcga cgacggtcca
     4561 gatctcggcc atcagatgaa cttgttcgcc tggaggaagt ccgccagctt ctcgtgggcg
     4621 tccccctcgt ccttcacgac ctcgcccttc tccttgggcg gcctgggcga ggcctccagc
     4681 accttcgtct tggagccctc cagacctacc tgctccgggt cgatcccgag ctcgtctccg
     4741 gccaggacct ggacctcctt ctgcttggcg gccatgatcc ccttcagcgt ggcgtagcgg
     4801 ggctcgttga tgcccttcac caccgagacg atggccggca gctcggcctc gaccacggcg
     4861 tgcccgccct cctcctcgcg gtgcacggtg accttgccgt ccgagacctc gatctgcttc
     4921 acgtaggtga gcgcggtcat gcccatcagc tggccgaggg cctgcggcac cagcgaggta
     4981 cgggcgtcgg tggactccga gcccagcagc atcaggtcga agtcctgctg ggcgagcgcg
     5041 agagccagca cgtaggcggt ggccatcgcg tcggagccgt ggagacggtc gtcggtgacg
     5101 aggatcccct tgtccgcccc cagcgccagc gccttgcgca cggcctcctt gtcggccggc
     5161 tccgggccca tcgtgaggac ggtcacctcg ccctcgccgg cggcgtcgcg gagcttgatg
     5221 gcctcctcga tcgcgtactc gtcgacgggg ttcatgatgg cctcgaccga ttcgcggtcg
     5281 agggtgaagt cctgctcgtc gagcttcttc tcggcagccg tgtccggcac gcgcttcacg
     5341 ccaacgacga tgttcaacag gttccctccg gatgtccgcc gcgtatccta gcgcatccca
     5401 ggctacctac ccacgggtag gctcgtcgac cccttccccc cgtcgaccct tcccccccgc
     5461 ggctacagtg acgggatgcg gcgcttccgg ctcgaggcgg tcctggtcgt cctgctcgtg
     5521 gccgcgctcg cgctcacgtt cgtcctgcga ggagcgccgg tgggcaccgg cggggacgac
     5581 gaccgcgaga ccgccgaccc gatgggaggg gccacgccgt gcatccaggc gctgttcggg
     5641 gagcccgtgc ccggcccgcc cgcggcgcag gccgacgtcg cctccgtggc cccggtcgtc
     5701 gaggagctcc gggagctcac cttcgaggag gagcccgcgc ccgtctacct cgaccccccg
     5761 gccctcagtg agcgcgtcac cgagatggtg gaggaggagg acgagttcct cgcgatccag
     5821 gcgcgactcc tcacggagat aggcgctctc cccgagggga tcgacctcga cgccgagatc
     5881 caggacctcc tcggggagca ggtggggggc ttctacgaca gcgatacagg cgagctcgtc
     5941 gtggccgacg acgcccggga gggcctcacc acggtcgagc tgctcacgct cgtgcacgag
     6001 ctccagca
//
LOCUS       DASUTZ010000266         5496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_105203, whole genome shotgun sequence.
ACCESSION   DASUTZ010000266 DASUTZ010000000
VERSION     DASUTZ010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105203"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VF097_11405"
     CDS             complement(<1..382)
                     /locus_tag="VF097_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263441.1"
                     /translation="MLVAISAYQLWFFVHLLGVFGLFLAHGISAGVALKLRSERDPVR
                     LDALLQLSGSAIPAFYVSLLVLLAGGIVAGFLGSWWRFGWIWASIGILVAIIVTMYAI
                     AGPYYRRVRTIVQAMARGSEAVTPQ"
     gene            complement(426..917)
                     /gene="folK"
                     /locus_tag="VF097_11410"
     CDS             complement(426..917)
                     /gene="folK"
                     /locus_tag="VF097_11410"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020396711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX6263442.1"
                     /translation="MPRAFLGLGSNLGDRLANLRRAVELLRGHGVRVLRSSRVYETEP
                     VGGPEQPDYLNAVIEVETAATARGLLETCISVEEEMGRVRVMRWGPRTIDLDLLTFGN
                     EEIDEPDLQVPHPRMHERVFVLAPLLELTAEPKLPGGRRVEDLRLGPELLLGARPFAA
                     PLA"
     gene            complement(917..1267)
                     /locus_tag="VF097_11415"
     CDS             complement(917..1267)
                     /locus_tag="VF097_11415"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: protein motif:HMM:NF014233.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX6263443.1"
                     /translation="MTTIRISGLRCRGRLGVTEEERERPQEIVVDLEIEVEAADDALD
                     ATADYDGVLRVVRGLVATESFVLLETLARRIAQIVASQPGASSCRATVHKPGAARALK
                     VDDVAASAVAGRGG"
     gene            complement(1264..1713)
                     /locus_tag="VF097_11420"
     CDS             complement(1264..1713)
                     /locus_tag="VF097_11420"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX6263444.1"
                     /translation="MAALDVSMTREEIEAYLLEQRTVRLATADASGVPHVVPLWFVWH
                     EGAMYLNSTLGNVTVRNMLAGGPATGVVDDGEAYDQLRGVTLTGEVERLGDTVPEDVQ
                     RAWSEKYLSGGELPYRRWRNRSWFRLSPTSTASWDFRKIPQGRARSR"
     gene            complement(1714..2532)
                     /locus_tag="VF097_11425"
     CDS             complement(1714..2532)
                     /locus_tag="VF097_11425"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6263445.1"
                     /translation="MTLERRTVEVSAGELAYVEAGEGKPILFLHGIPTSADLWRNVAP
                     LLALWARVIVPDLLGYGASAKPEGAELHIRAQAGYARDLLDALGIERFAVVGHDLGGG
                     IAQLLAIEGGVEALVLTNSISWDGWPVEGVRKVQDLPEEGWDEALAEKVVRRSFERGM
                     ARFERLAPDDLEGYVAPWREDPLALRRAFLSVDGVGLEGTVEALGALGIPAFVLWGED
                     DPFLPAERAERLGELMPASTTALLPGVSHYLMEDAPETVGPLVSQWLRTTYLEG"
     gene            complement(2529..3362)
                     /gene="folP"
                     /locus_tag="VF097_11430"
     CDS             complement(2529..3362)
                     /gene="folP"
                     /locus_tag="VF097_11430"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX6263446.1"
                     /translation="MTTWKLPDRTYDCDERTLVMGVLNVTPDSFSDGGRFFDPEDAVK
                     QVLRMVEDGADIVDVGGESTRPGSDPVPPDEERRRVVPIVERLAQELEVPISIDTRKA
                     EVAAAAVRAGATIVNDVTAGRDREMFDVVRESGAALVLMHMKGEPKTMQEDPHYDDVV
                     REVRDFLAERVEAAVAAGVDREAICVDPGLGFAKTTPHSLAMMRHTDALLDLGRPVLV
                     GPSRKSFIGDVLGTEVTERAEGTVGAVCYMVARGAHIARVHDVRETVRAVRVVDAILR
                     A"
     gene            3441..4124
                     /locus_tag="VF097_11435"
     CDS             3441..4124
                     /locus_tag="VF097_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6263447.1"
                     /translation="MTEQRVLVVDDEPIVREVLGRYLEREGFEVGTAADGEEAIERFA
                     DAPPDLVVLDLMMPKLDGAGVLSWIRRRARTPVIMLTARGEETDRVVGLEMGADDYVT
                     KPFSPREVIARVRAVLRRSEGGGAADGRVRHGDLEIDPWARRATIRGAALDLTPKEFD
                     LLHHFASNPGVVFSRTELLEDLWDFAFDGDPSTVTVHVRRLREKIEDDPSRPVHLVTV
                     WGVGYRFDP"
     gene            4121..5323
                     /locus_tag="VF097_11440"
     CDS             4121..5323
                     /locus_tag="VF097_11440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6263448.1"
                     /translation="MSTKALLGLGLLGAAGAGATVAIAAATGMPRADLAELSILLAVA
                     VLATVGVTAAAARLLARSSFRVRIAAASLAGVAVALLNLGAFALLMFVSGHDAALTAV
                     LLAYTGTVGVAAGLALARSSRDGVARLEGTARRLAEGDLDARVGRVEAGPELDTLART
                     LDEMASRLQEAQRREREAESRRRDLVAAVSHDLRTPLAGLRAMVEAVEDGVVDDPRDL
                     RRYTREMLRAVGALSTLVDDLFELSQLDADAIREEARRASLADVVASALAAVEPQRLA
                     KGVSVETNLAGAATEPCSPRVTRVLQNLIQNAIRHTPADGSVRVEARRDAGRLEIAVT
                     DTGEGIPVDSLDQVFEPFWRGDAARSGPGSGLGLALAKRLVMAMGGDISVESRPRQGS
                     RFAVLLPT"
BASE COUNT          770 a         2010 c         1987 g          729 t
ORIGIN      
        1 gctgaggcgt cacggcctcg gagccgcggg ccatggcctg gacgatcgtc ctcacgcgcc
       61 ggtagtaggg gccggcgatc gcgtacatcg tcacgatgat agccacgagg atcccgatcg
      121 aggcccagat ccagccgaac cgccaccagc tccccaggaa cccggccacg atgccgcccg
      181 ccagcaggac gagcagggac acgtagaacg cggggatcgc cgagcccgag agctggagga
      241 gcgcgtcgag gcggaccgga tcgcgctcgc tgcggagctt gagggccacg ccggccgaga
      301 tcccgtgcgc caggaacaga ccgaacaccc ccaggaggtg cacgaagaac cacagctgat
      361 aggcggagat ggccacaagc acggcgaaac cctgccccac cttgccgccg gggtcaactc
      421 gcgggtcagg ccagcggcgc ggcgaagggg cgggcgccca gcagcagctc ggggccgagg
      481 cggagatcct cgacccgccg gcccccgggc agcttcggtt cggccgtcag ctcgagcagc
      541 ggcgccagca cgaagacccg ctcgtgcatc cgggggtggg ggacctggag gtccggctcg
      601 tcgatctcct cgttcccgaa ggtgaggagg tccaggtcga tcgtgcgcgg gccccaccgc
      661 atcacacgga cccggcccat ctcctcctcc acggagatgc acgtctcgag caggccgcgc
      721 gcggtggccg cggtctcgac ctcgatcacc gcgttgaggt agtcgggctg ctcgggacct
      781 cccacgggct cggtctcgta cacgcgggac gaccggagga cccggacgcc gtgcccccgg
      841 aggagctcga ccgcgcgccg gaggttggcg aggcgatcgc ccaggttgga tccgaggccg
      901 aggaaggccc ggggcatcag ccgccgcggc cggcgacggc cgacgcggcc acgtcgtcga
      961 ccttcagcgc tcgggcggcc cccggcttgt gcacggtggc ccggcacgag gacgcccccg
     1021 gctgggacgc gacgatctgg gcgatccgcc gggccagggt ctccaggagg acgaaggact
     1081 cggtggccac gaggccgcgg acgacgcgca ggaccccgtc gtagtccgcc gttgcgtcca
     1141 gcgcgtcgtc cgcggcctcg acctcgatct cgaggtcgac cacgatctcc tggggccgct
     1201 cccgctcctc ctcggtgacg cccaggcggc cccggcaccg gagcccggag atgcggatcg
     1261 tcgtcatctc gacctcgccc ttccctgggg gatcttgcgg aagtcccagg acgcggtgga
     1321 ggtcggcgag aggcggaacc aggaccggtt cctccaccgg cggtacggca gctcgccccc
     1381 gctcaggtac ttctcggacc aggcccgctg cacgtcctca ggcacggtgt cgccgaggcg
     1441 ctccacctcc ccggtgaggg tcacgccccg gagctggtcg taggcctcgc cgtcgtccac
     1501 gacgcccgtg gccgggccgc cggccagcat gttccggacc gtgacgttcc ctagggtgga
     1561 gttgaggtac atcgcgccct cgtgccagac gaaccacagc ggcaccacgt gggggacgcc
     1621 cgaggcgtcg gcggtggcca gccgcaccgt gcgctgttcg aggaggtagg cttcgatctc
     1681 ctcccgggtc atcgagacgt cgagcgcggc cactcagccc tccagatagg tggtgcggag
     1741 ccactggctc acgagcggtc ccaccgtctc gggggcgtcc tccatcaggt agtgcgagac
     1801 gccggggagg agggcggtgg tggaggccgg catgagctcg cccaggcgct cggcgcgctc
     1861 ggcggggagg aacgggtcgt cctcgcccca gagcacgaag gccgggatgc cgagcgcgcc
     1921 gagcgcctcc acggtcccct cgagccccac gccgtccacc gacaggaagg ccctccgcag
     1981 cgcgagcggg tcctcccgcc acggggcgac gtatccctcg aggtcgtccg gtgccaggcg
     2041 ctcgaacctg gccatcccgc gctcgaacga ccgccgcacg accttctcgg ccagcgcctc
     2101 gtcccaaccc tcctcgggca ggtcctgcac ctttcgcacg ccctccaccg gccacccgtc
     2161 ccaagagatc gagttcgtca ggacgagtgc ctcgaccccg ccctcgatcg ccagcagctg
     2221 cgcgatccct ccccccagat cgtggcccac gaccgcgaac cgctcgatcc cgagagcatc
     2281 gaggaggtcg cgcgcgtacc cggcctgcgc ccggatgtgg agctccgccc cctcgggctt
     2341 ggcggatgcc ccgtacccca gcaggtcggg gacgatcacc ctggcccaga gcgcgaggag
     2401 cggcgccacg ttgcgccaga gatcggcgct cgtggggatc ccgtgcagga agaggatcgg
     2461 cttcccctcc cccgcctcga cgtacgcgag ctcccccgcc gagacctcca cggtccggcg
     2521 ctccagggtc atgcccgaag gatcgcgtcc acgacccgaa cggcgcggac ggtctcccgg
     2581 acgtcgtgga cccgcgcgat atgtgcgccc ctggcgacca tgtagcagac cgcccccacg
     2641 gtgccctcgg cccgctcggt cacctcggtc ccgaggacgt cgccgatgaa cgacttccgc
     2701 gaggggccga ccagcacggg ccgccccagg tccagcaggg catcggtgtg cctcatcatc
     2761 gccagggagt gcggcgtggt cttggcgaag cccaggcccg ggtcgacgca gatcgcctcc
     2821 cgatcgaccc ccgcggcgac cgccgcctcg acccgctcgg ccaggaagtc ccggacctcc
     2881 cggaccacgt cgtcgtagtg ggggtcctcc tgcatcgtct tcggctcgcc cttcatgtgc
     2941 atcagcacga gggcggcgcc gctctcccgg accacatcga acatctcgcg gtcgcggccc
     3001 gcggtgacgt cgttgacgat cgtagccccg gcccggacgg cggccgcggc cacctcggcc
     3061 ttcctcgtgt cgatcgagat cgggacctcc agctcctggg ccagccgctc cacgatcggg
     3121 accacgcggc gccgctcctc gtcgggaggc acgggatcgg acccgggccg cgtggactcc
     3181 ccgcccacgt ccacgatgtc ggccccgtcc tcgaccatcc ggaggacctg cttcaccgcg
     3241 tcctcggggt cgaagaaccg gcccccgtcc gagaaggagt cgggggtgac gttcagtacg
     3301 cccatcacca gcgtgcgctc gtcgcagtcg taggtccggt cgggcagctt ccaggtcgtc
     3361 acggccacag cgtacccatg cgggccccgg gccgcccacc tcgtaaggtc ttcgtaagga
     3421 aggtgaggga ggatggacgc gtgaccgagc agcgagtgct cgtcgtggac gacgagccca
     3481 tcgtgcgcga ggtgctgggg aggtacctgg agcgcgaggg cttcgaggtg ggcacggccg
     3541 ccgacggcga ggaggcgatc gagcgcttcg ccgacgctcc gcccgatctg gtcgtcctcg
     3601 acctcatgat gccgaagctc gacggggccg gggtcctgtc gtggatccgc cgccgggcgc
     3661 ggacccctgt gatcatgctg accgcccggg gcgaggagac cgatcgcgtg gtggggctgg
     3721 agatgggcgc cgacgactac gtgacgaagc ccttctcgcc gcgagaggtg atcgcccggg
     3781 tgcgggccgt cctccgccgc tcggaaggtg gaggcgcggc cgatggccgg gtccgccacg
     3841 gagacctgga gatcgacccc tgggcccggc gggccacgat ccgaggtgcg gcgctcgatc
     3901 tcacccccaa ggagttcgac ctgctccacc acttcgcctc gaacccgggg gtggtcttct
     3961 cgcggaccga gctcctcgag gacctctggg acttcgcgtt cgacggcgac ccctcgacgg
     4021 tgaccgtcca cgtccggcgg ctgcgggaga agatcgagga cgatccctcc cgccccgtcc
     4081 acctcgtgac cgtgtggggt gtcggctacc ggttcgatcc atgagcacga aggcgctgct
     4141 cgggctggga ctgctggggg ctgcgggggc cggggccacc gtggcgatcg cggcggccac
     4201 ggggatgccg cgggccgacc tcgccgagct ctcgatcctc ctggccgtcg cggtcctggc
     4261 caccgtgggc gtcacggccg ccgcggcccg gcttctggca cggtcgtcgt tccgggttcg
     4321 gatcgccgcg gcgtcgctgg cgggggtggc cgtggcgctg ctcaacctcg gagcattcgc
     4381 gctcctgatg ttcgtcagcg ggcacgacgc cgcgctcacg gccgtcctgc tcgcctacac
     4441 ggggacggtg ggcgtcgcgg ccggcctggc cctggcgagg agctctaggg acggcgtcgc
     4501 ccggctcgag gggacggctc gacgactggc ggagggggac ctggatgccc gggtgggtcg
     4561 cgtggaggcc gggcccgaac tggacaccct ggcccgcacc ctcgacgaga tggcctcccg
     4621 gctccaggag gcccagcgga gggagcggga ggcggagtcg aggcgccggg acctcgtggc
     4681 cgccgtctcc cacgacctga ggacgccgct ggccggcttg cgagcgatgg tggaggccgt
     4741 ggaggacgga gtggtcgacg atccccggga cctcaggcgc tacacgcgcg agatgctgcg
     4801 ggccgtgggc gccctgagca ccctcgtgga cgacctcttc gagctgtccc agctcgacgc
     4861 cgacgccatc cgggaggagg cgcggcgggc cagcctggcc gacgtggtgg cgtcggccct
     4921 cgccgccgtg gagccccagc gcctggccaa gggggtctcg gtggagacga acctggccgg
     4981 cgcggcgacc gagccgtgct cgccccgagt cacccgcgtc ctgcagaacc tcatccagaa
     5041 cgcgatccgg cacacgccgg ccgacggcag cgtccgggtc gaggcccggc gggacgccgg
     5101 ccggctcgag atcgcggtca ccgacaccgg ggagggcatc ccggtggact ccctggacca
     5161 ggtgttcgag ccgttctggc gaggcgacgc cgcccgatcc gggccggggt ccggcctggg
     5221 cctcgccctg gccaagcggc tggtgatggc gatgggcggc gacatcagcg tggagagccg
     5281 accccgccag gggtcgcgct tcgccgtgct cctgccaacc tgagcggtcc ccccggaacg
     5341 gaaccttcct cggctctgag gcgtcttctg gtcggaggat gggccatgat gcagacgagg
     5401 gcgttgagcg tgaccgagac ggggaggctg ggggagctgt acgagcggca cgcccccgac
     5461 gccgtccggc tcgggtacct gatcaccggc gacgcc
//
LOCUS       DASUTZ010000267         3429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1011408, whole genome shotgun sequence.
ACCESSION   DASUTZ010000267 DASUTZ010000000
VERSION     DASUTZ010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011408"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /gene="rplU"
                     /locus_tag="VF097_11445"
     CDS             <1..494
                     /gene="rplU"
                     /locus_tag="VF097_11445"
                     /inference="COORDINATES: protein motif:HMM:NF013025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX6263449.1"
                     /translation="QMYAVIKTGGKQHKVRPGDVIEVEYMDVDPGSAVEFEPILVMDD
                     KNRPHVGKSLGNAKVVATLVGDKKADKIKVVKYRAKSGYKRNQGHRQRMTVLEIQEVK
                     LGGRAGKKDEEETKPAAKKPAAKKSTRKKAAAKKTSAKKSSAKKSSKKSASKKPSSRK
                     KAT"
     gene            514..762
                     /gene="rpmA"
                     /locus_tag="VF097_11450"
     CDS             514..762
                     /gene="rpmA"
                     /locus_tag="VF097_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011207884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX6263450.1"
                     /translation="MAHKKGAAASRNGRDSTSKRLGVKAFGGETVKAGEIIVRQRGTK
                     VHPGLNVRRGGDDTLFAVAPGNVRFYSSNGRKYVTVEA"
     gene            complement(746..1132)
                     /locus_tag="VF097_11455"
     CDS             complement(746..1132)
                     /locus_tag="VF097_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263451.1"
                     /translation="MPKRTIAWLWRPGESKERRGTLSLEGGTATFRSDDGEQVRFGDV
                     ERARRQRGTPVLEIRYLREGKRRAAFLFFARPPERPDPEPVPSLFGVIGIRGMRRVGD
                     MGRIRAANRRLKPLIEEWEEALRPRR"
     gene            1153..2391
                     /gene="obgE"
                     /locus_tag="VF097_11460"
     CDS             1153..2391
                     /gene="obgE"
                     /locus_tag="VF097_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX6263452.1"
                     /translation="MSEFVDEAVVYARGGRGGDGAASFHREKYRPKGGPDGGDGGRGG
                     DVILEASPDVFDLASLARHPHQRAEDGGHGSGDNRHGADGDDLVIPVPDGVFVEEERG
                     PVADLVGPGARVVVARGGRGGRGNASLASGRNRAPQTAERGEPGEERRLRLELRLVAD
                     VGLVGIPNAGKSTLLAALTAARPKVADYPFTTLTPNLGVAEGERRVVLADVPGLIEGA
                     HEGRGLGHRFLRHVSRSRALAFVVDVAGDDPVADLRLVRAEVAAYATELAERPWLVAA
                     TKADLLDAEEAERRAEALREEGETHLVSGRTGEGLERLAERLGHLADQSTVERKPFVV
                     LRPGREPFMVRREGDGFRVEGRIVERWVEETDLDDPAQVVSLQDRLRREGVERKLEEL
                     GARGGEEVRIGDRAFEYHPE"
     gene            2401..2832
                     /locus_tag="VF097_11465"
     CDS             2401..2832
                     /locus_tag="VF097_11465"
                     /inference="COORDINATES: protein motif:HMM:NF019632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6263453.1"
                     /translation="MSKDELFRLEQAAWAPLHDLLHGLNPEAADRPYTDLWTVKDGLA
                     HIACWQAEAAQMLLQMREGTYEGWEDGHEEEINARFHEATRELPLDEVIASLHASRSR
                     MLEELDRLPDDLLDDEARSWFVESGHEHYEEHLEGLRAVLG"
     gene            2907..>3429
                     /gene="nadD"
                     /locus_tag="VF097_11470"
     CDS             2907..>3429
                     /gene="nadD"
                     /locus_tag="VF097_11470"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012183886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX6263454.1"
                     /translation="MGGTFDPIHQGHLVTAEEALWQFELDQVLFVPTGQPWMKHEPGV
                     SPAEDRYLMTVIATSSNPRFRVSRLEIDREGPTYSVDTLRSLREEYGEGTELFFITGA
                     DAILQIFQWKDADQAFELAHFIAATRPGYDLSRFDQERAAEHPKITVMDIPALAISST
                     DIRERVAKGRPIRY"
BASE COUNT          574 a         1204 c         1224 g          427 t
ORIGIN      
        1 gccagatgta cgcggtgatc aagacgggcg ggaagcagca caaggtccgc cccggggacg
       61 tgatcgaggt cgagtacatg gacgtggatc cgggctcggc cgtggagttc gagccgatcc
      121 tcgtcatgga cgacaagaac aggccccacg tgggcaagtc gctggggaac gcgaaggtcg
      181 tggccaccct cgtcggcgac aagaaggccg acaagatcaa ggtcgtgaag taccgggcca
      241 agagcggcta caagcggaac caggggcacc gccagcggat gaccgtcctg gagatccagg
      301 aggtgaagct cggcggtcgc gccgggaaga aggacgaaga ggagacgaag cccgcggcca
      361 agaagcccgc ggccaagaag tcgacgagga agaaagccgc cgccaagaag acctcagcca
      421 agaagtcgtc ggccaagaag tcctcgaaga agagcgcctc caagaaaccc tcgagccgga
      481 agaaggccac ctaggctctg ctatcctggg gccatggctc acaagaaggg cgccgctgcg
      541 tccaggaacg gccgcgacag cacctccaag cggctcggcg tgaaggcctt cggcggggag
      601 accgtgaagg ccggcgagat catcgtgcgc cagcgcggca ccaaggtgca ccctgggctg
      661 aacgtgcgcc ggggcggcga cgacacgctc ttcgcggtgg ccccggggaa cgtgcggttc
      721 tactcctcga acggccgcaa gtacgtcacc gtcgaggcct gagggcctcc tcccactcct
      781 cgatcagcgg cttcagccgc ctgttcgccg cccggatccg ccccatgtcc ccgacgcgcc
      841 tcatccctcg gatcccgatg accccgaaga ggctcggcac cggctcgggg tcggggcgct
      901 cgggaggacg ggcgaagaag aggaacgcgg cgcgccgctt gccctcgcgc aggtagcgga
      961 tctcgaggac cggcgtcccc cgctgccggc gggctcgctc cacgtccccg aaccggacct
     1021 gctcgccgtc gtccgaccgg aaggtggcag tccctccctc gagcgagagc gtgccccgac
     1081 gctccttcga ctctccgggg cgccacagcc aggcgatcgt ccgcttgggc acggccccta
     1141 tactcgcacc gcatgtccga gttcgtggac gaggccgtgg tctacgcgcg cggcggccgc
     1201 gggggcgacg gcgccgcgtc gttccaccgc gagaagtacc ggcccaaggg tggccccgac
     1261 gggggcgacg gcggccgagg cggcgacgtg atcctggagg cctcccccga cgtgttcgat
     1321 ctggcctcgc tggcccgcca cccacatcag cgggccgagg acggcggcca cgggagcggc
     1381 gacaaccggc acggggccga cggcgacgac ctcgtgatcc cggtgcccga cggcgtcttc
     1441 gtcgaggagg aacggggccc ggtggcggat ctggtggggc ccggggctcg cgtcgtggtg
     1501 gcccgcggcg gccgcggcgg gcgagggaac gcctcgctgg cctccgggcg caaccgcgcg
     1561 ccccagacgg cggagcgggg ggagccgggc gaggagcgac ggctccgtct cgagctacgg
     1621 ctcgtggccg atgtgggtct cgtgggcatc cccaacgccg gcaagtccac cctgctggcg
     1681 gcgctcaccg cggcccggcc caaggtggcc gactacccgt tcacgaccct cacccccaac
     1741 ctcggcgtgg ccgaggggga gcggcgcgtc gtgctggcgg acgtcccggg attgatcgag
     1801 ggggcgcacg aggggcgagg cctcggacac cggttcctcc gccacgtctc ccggtcgcga
     1861 gcgctcgcat tcgtggtgga cgtcgccggc gacgaccccg tggccgacct ccggctcgtc
     1921 cgggccgagg tggccgccta cgccaccgag ctcgccgagc gtccctggct ggtcgccgcc
     1981 acgaaagcgg acctgctgga cgccgaggag gccgagcgac gggccgaggc gctccgcgag
     2041 gagggtgaga cccacctcgt gtcgggccgg acgggggagg gcctcgagcg cctcgcggag
     2101 cgcctcggcc acctcgccga ccagagcacg gtggagcgga agcccttcgt cgtgctccga
     2161 cccggccggg agcccttcat ggtccgccgc gagggcgacg gcttccgcgt cgaggggcga
     2221 atcgtcgagc gctgggtgga ggagacggac ctcgacgatc ccgcccaggt cgtctcgctc
     2281 caggaccgcc tccgccgaga gggcgtggag cggaagctcg aagagctcgg agcccggggt
     2341 ggcgaggagg tccgcatcgg cgaccgcgcc ttcgagtacc accccgagta gcatccggtc
     2401 atgagcaagg acgagctctt ccgcctcgag caggccgcgt gggctcccct ccatgacctc
     2461 ctgcacggcc tcaacccgga ggccgccgac cgcccctaca cggacctctg gacggtgaag
     2521 gacggcctgg cccacatcgc gtgctggcag gccgaggccg cgcagatgct gctccagatg
     2581 cgggagggca cctacgaggg atgggaggac ggacacgagg aggagatcaa cgctcgcttc
     2641 cacgaggcaa cgagggagct ccccctggac gaggtgatcg cgagcctcca cgcctcccgt
     2701 tctcggatgc tcgaggagct cgaccgtctg cccgacgacc tgcttgacga cgaggcccgc
     2761 tcctggttcg tggagagcgg ccacgagcac tacgaggagc acctcgaggg gctcagggcc
     2821 gtcctcggat agccggcgaa atccacggga caccgcaggt ccggtcatac aatcgccgcg
     2881 tggacgcccc caaccggctc ggcgtcatgg gcggcacctt cgacccgatc caccagggcc
     2941 acctcgtcac cgccgaggag gcactctggc agttcgagct ggatcaggtc ctcttcgtgc
     3001 cgaccggaca gccgtggatg aagcacgagc ccggggtgtc cccggcggag gaccgctacc
     3061 tgatgaccgt gatcgcgacc tccagcaacc cgaggttccg ggtgtcgcgc ctcgagatcg
     3121 accgcgaggg ccccacctac tccgtggaca ccctccgctc gctgcgcgaa gagtacggcg
     3181 aggggaccga gctgttcttc atcaccgggg cggacgccat cctccagatc ttccagtgga
     3241 aggacgcgga ccaggcgttc gagctggccc acttcatcgc cgcgaccagg cccggctacg
     3301 acctctcgcg attcgatcaa gaaagggccg ccgagcatcc gaagatcact gtgatggaca
     3361 tcccggccct ggcgatctcc tccacggaca tccgggagcg cgtcgccaag ggcaggccca
     3421 tccggtacc
//
LOCUS       DASUTZ010000268        17123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_753314, whole genome shotgun sequence.
ACCESSION   DASUTZ010000268 DASUTZ010000000
VERSION     DASUTZ010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 17123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17123
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753314"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /locus_tag="VF097_11475"
     CDS             complement(<1..1207)
                     /locus_tag="VF097_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263455.1"
                     /translation="MGITPVRSEAVWGHLRVLFTGAALLFLINIWFGFDNTLTSGGIP
                     RWQALIHLHAGSIGWITLSAIGIGIWVFTGSRVVSRDYERRVGFLVWTAVLVFAGYIP
                     NFWLAFSRGDDPLVALLPIFGSLAVIVLWISAIFVLRQLPHQEVVTTVHVLVAAALLV
                     AAIGATMGALLGLERVIGEFLPIVDPDRVGVHAGMMDTYLFLLAAAIIEWTIAQDPMR
                     RWTRAGLAQGLAWGVGATLVPLAFLLDLVDQLLPVFGLLLLLGLVLFLGRVAWRALAV
                     GPAAQEASVRWAFFGTAWLIAFLGLFLYAVGSGDFDSLPSWFGAVFVHAGFVGMMTNL
                     ILGVLSARTNERRHVLAWGEPAALWTINLGLVLFLGLKIAADVHIGALAMGLGVLLGV
                     GTMLARMGSG"
     gene            complement(1213..2406)
                     /locus_tag="VF097_11480"
     CDS             complement(1213..2406)
                     /locus_tag="VF097_11480"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6263456.1"
                     /translation="MTLSAEELAARAGTTAEQIRRLADLRILPSEPRFEEALVPRVRL
                     ALELEASGIPLEQIGRRSGREGFSLSFADRVFRRPVAMLPTTYAELLEELQMSERLFD
                     DVRVSLGVTGDEPVREDDAELLRLLDRMIELGLDEPSLIRFVHSVVDSTRRMADAGRE
                     LWRTGVEAPLLASGLSHQELLQASADPAPESQVIGDRLIGILWNRFIEQVVVQASIEH
                     LELALEEAGVAFRRNPRPPAIAFVDLSGYTQMTDRVGDHAAAEQARAMLDAVRRTIGG
                     SPGRLVKMLGDGAMLHFPEAGAAVTCALRLIDRIPRSGLPPARLGIDAGPLIARDADF
                     FGRTVNVAARLADYARPREVLVTSSVVDASGDAPASFREIGSVALKGVSEPVTVFSAQ
                     PTEGS"
     gene            complement(2403..3053)
                     /locus_tag="VF097_11485"
     CDS             complement(2403..3053)
                     /locus_tag="VF097_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263457.1"
                     /translation="MTDLDRSSTTTVTRIAPGTGLAFVGLALAAALVIGQYSYLPAGD
                     EIAAFFQENARRVQIGAYLGALSSVFLIWFSGSVRSHLRVSEGGTGRLSTVAFGGGVA
                     GAVGTGIAFSILSVAGARGSEGQVTPATAMVLYDIYGSLIGVAVPITLAALLGASSLV
                     ALRAGALPGWLAWSGAVLTIGLISPFSYIFIAIAFLWIAVVSILLLLSSEGAITSR"
     gene            complement(3050..3514)
                     /locus_tag="VF097_11490"
     CDS             complement(3050..3514)
                     /locus_tag="VF097_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263458.1"
                     /translation="MVTMRRTGRRRARDAITRERAGRALFGVVSAALLLSGTYLVVNT
                     VPDADDLRRSGISWEELLANRPAVAVYLERLLRLTGVAAAGLGTMALAGALSMRSAGR
                     PVWRMLWTLPAITAAITSVLVAEGNPIAWYYGGLTLLAAAGLVLARPGKEEG"
     gene            3681..5681
                     /locus_tag="VF097_11495"
     CDS             3681..5681
                     /locus_tag="VF097_11495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX6263459.1"
                     /translation="MRRVAMWGLAGIGAACLVITVVVALSSNLEPGVAAWIFVGPLPI
                     LGMGAFLYWRRPDHRTSQQFLVWAVVTFCLAMPIEAFTVAAVEGGSPPSWTWLAVLVF
                     MTASVVGAVLGATLIGLLPDGRFRHPWERPILLPMWGMLVVPLLAFLTNERVPLPGYV
                     FPGIDDASSPLFVPSLAALGPVAMTLNALYSVVFVAGMGLLVVRYRKGGGRERKQLRW
                     VLLAAVVTVGAAIWPFVLGELGILPAVTHGIWATVLWLPILLLPISIVVAVLEPAWLD
                     VDLAIRKTFVYGTLSFLILALYVGVATVSGVAARAALPLEVAILVAVGVAALFQPVRR
                     WLQSVADRWVFGEPPAGYEAVTDFTTAVKEATDPGDLVPRIADTTRRALGLRWVGATV
                     EGERPVVLGERTGRPELVAPIEHQGDRFGRIECGPKSKGAFNEADVELVTALASQAAL
                     ALFNARLAGRIVHTQEAERRRIERNIHDGAQQELVALVARLGMARADLRRDRLSEETL
                     VELQREASRILSDLRELAQGIHPSVLSDGGLLEAIEDRCGRLPIDVSLEATPGLRARR
                     FPDEVEGAAYFFVTEGVANVLKHAQASEARIAVRHEDGVLALDVSDDGVGFDPWRVRR
                     TGLSGLSDRIAALGGTFAVTSRPGEGASLRAVLPIVPPGASA"
     gene            5678..6373
                     /locus_tag="VF097_11500"
     CDS             5678..6373
                     /locus_tag="VF097_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019158607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6263460.1"
                     /translation="MSDRLRVVIAEDHYLVREGTRGLLESSGEVEVAAAVEDAPSLLD
                     AVERLHPDVVLVDIRMPPTHQTEGIDAARRIRAVHPEVGVVVLSQHVSSHYAFELFRD
                     GTSGLAYLLKDRVGDLDELLAALRSVADGGSVIDPLVVERLLAQGERLSGTGLSELSP
                     REMEVLAKMAEGKSNAAIAGALFVSQSAVEKHINSIFAKLGLSVDEGETHRRVAAVLA
                     FLRSQDQSRETLG"
     gene            6370..7311
                     /locus_tag="VF097_11505"
     CDS             6370..7311
                     /locus_tag="VF097_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004559817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6263461.1"
                     /translation="MSALLRVAGLRKSYGAIEALRGVDLEVLPGEIVALLGPNGAGKT
                     TLVSIVAGLRRPDGGTVEVDGLDALRRSADVRRRIGLAPQELGIYPVVSVRSNLALFG
                     RLAGLSGGQLRSRIEDVAEALRITDLLDRQGGSLSGGQKRRVHTAIALLHRPPLLLLD
                     EATTGADVETRGHILNLVRRLAGDGSAVLYSTHYLQEVEDLGATVVLLDGGRVIARGG
                     AAELIAAHATTELEMTFEGDVPALGLNGSATVEGSRLRLPAQDPAATLATLIPRVPPG
                     TLRSVEIVRPDLEAVYLALTGRRYEEADEEGERVAAG"
     gene            7295..8068
                     /locus_tag="VF097_11510"
     CDS             7295..8068
                     /locus_tag="VF097_11510"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6263462.1"
                     /translation="MSPRASVAIMRHEFRVLMTDPSTFVFLIVMPLVMAALTKQLFQG
                     ALAAEGFRGANGSEFAIPGMAVSFAAFGVGYAGFTFFRDHGWGTWDRLRATPASSLDL
                     IAGKTVPVLLVTGLQLGLLFALGGPLLGLRVTGSGVALAVTIVALALALAAFGLAVTA
                     VARTMQQLNAIGSVGGFAMAIIGGAFVPVSSMPDWAQAVAPVMPTYWAMKAFTSVILE
                     GGGLGAVAVPAMMMLGFAVVFALLVAAKFRLEDTKAYYG"
     gene            complement(8084..9175)
                     /locus_tag="VF097_11515"
     CDS             complement(8084..9175)
                     /locus_tag="VF097_11515"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012945108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6263463.1"
                     /translation="MSSERIDTIVIGGGQAGLSVGYHLARRGVPFLILDARERVGDAW
                     RNRWDSLRLFTPNRFNGLPGMRFPGPRWRFPSKDEFADYLESYALRFELPVRRGMRVD
                     HLTRNGDRFVATAGDRSFETENVVIAMSSWQRPRVPDFASELDPGILQLHVADYRNPK
                     QLRDGDTLVVGAGNSGAEVAMDLAGSRRVWLSGPDTGHLPFRPETLVGRVLMPFVGRV
                     IFHRVLTTSTPMGRRARPKMLKKGDRLIRVKPKDLAAAGVERVPRTTGVEEGLPQLED
                     GRRLDVANVIWCSGFHPGFSWIDLPVLGPDEPKHERGVVDAEPGLYFVGLKFLYAKSS
                     EQIHGVGRDAARIADLIAAGRRQGVETAG"
     gene            complement(9333..10895)
                     /locus_tag="VF097_11520"
     CDS             complement(9333..10895)
                     /locus_tag="VF097_11520"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX6263464.1"
                     /translation="MSQQPAVAPESVRAAEIIAAACLATDLGMGFPFEHGLHGTLMAM
                     RLAELLDVDRDTAWRTYHVSLLMYSGCITDADVATKIFAGRRTENVTPAQFGSSSEAL
                     AGVVRALPDPEVSAPVRLTQAAGRLMTAARFRKTHFASLCEVAEMMAKRLGLPAEVHG
                     LFAHLTERWDGKGLLKRGRGEEIPLPLRIVHVARDAAYQRVVGGDERAVDSTRRRAGR
                     AFDPTIARRLVEHAPEIFGAADEPTSAWEATLDAEPHPRLTLEDGQVDGALAAIGNFA
                     DLVSAHLSGHSRGVADLAASAAKICGFEGNEHALVRRAALVHDVGRVAIHPRIWQKPG
                     PLSVDEWEHVRLHPYHSERVLVRSPLLASLAEIACAHHERLDGEGYHRRVAASTLGAG
                     ARLLAAADAFHAMCEPRPHREAIPPERAAELLGEEARAGRLDPQMVAAVVEAAGQPPP
                     PIERPAGLTEREVEVLGLLARGLQTKQVARRLGISVKTADRHIQNAYRKAGVSTRAAA
                     TMFAMQHGLVPV"
     gene            11000..11623
                     /locus_tag="VF097_11525"
     CDS             11000..11623
                     /locus_tag="VF097_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020026641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HEX6263465.1"
                     /translation="MDDYPVRFDVDYPDRALDRLTTFFRLVWAIPILILLGTIAGGTW
                     QWSYEESRYVAAGAGGLLFFGPLLMIVFRQKYPRWWFDWNLAWLRFSNRVSAYLALLD
                     DRYPSSDEEQAVHLEIPYPDAQEDLNQFLPLVKWFLAIPHYIVLVFLYVGGVFAVIFA
                     WFAILFTGRYPRGLFDFVVGVFRWQNRVVGYAFVLVTDRYPPFSLAG"
     gene            11688..11984
                     /locus_tag="VF097_11530"
     CDS             11688..11984
                     /locus_tag="VF097_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263466.1"
                     /translation="MRVDRRSSTHVLLVLALVFLAIVLLWHLFGMDHSERMGILGGCL
                     FLLTAALVLVVPAIRWPLPAPTEARREGPPTAVLHPVSRPPPIEASLASVVLIN"
     gene            12037..12579
                     /locus_tag="VF097_11535"
     CDS             12037..12579
                     /locus_tag="VF097_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263467.1"
                     /translation="MSYPTRTIRWWAGIAVVLVTVLLATACADTETGDGAEGGTVDVT
                     LQEWSVVPGQDSVAAGPVTFNVTNEGPDHPHELVVIRTDLDPAELPTTDEGGVDEDGE
                     GIEVIGEIEEFPPGETHDATFDLEPGSYVLVCNVVAVAGEHGEGHEEEEEMEEEGEHE
                     GMEEMEIHYMLGMRAPFTVT"
     gene            12700..13104
                     /locus_tag="VF097_11540"
     CDS             12700..13104
                     /locus_tag="VF097_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263468.1"
                     /translation="MRMGRLLWGLVAFWGAIGAVLAVVALSQPPAEESPPAAAPPALR
                     TVVVSEAAPAQPSSPDGPVEVALLGGDAPSEPGMATVLSDENCEPDANGYSHCLNELE
                     MEDGERIAVRHTHRMAEVPCLAPGERVNVRLS"
     gene            complement(13091..14470)
                     /locus_tag="VF097_11545"
     CDS             complement(13091..14470)
                     /locus_tag="VF097_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6263469.1"
                     /translation="MTLKQAIPDLRDRFTGRVMGPEDEGYEEARTVVAGDVDKRPAVI
                     VRPTDAEDVARVIALARETGLDLAVRSGGHSGAGHSATEGGIVLDLRAMKGLEIDVKG
                     RTAWAEAGLTAGEYTAAAGGYGLATPFGDTGTVGIGGITLAGGIGLLVRRYGMTIDSL
                     LAAEIVTADDQLLRVDEEHHPDLFWAIRGGGGNFGVVTRFLFRLHPVDRVMGGMLLLP
                     ATAEVVAGFIVEAERAPEELSTIANVMPAPPMPFVPEERHGELVVLALMVHAGDVGEG
                     ERAVAPFRSLAEPVADLLRPMPYPEMFPPEQEEYRPTAIAQTMLIQEVDAAAAEMIVE
                     RLEASDAAMRAAQLRVLGGAMARVPADATAFAHRDARIMGNLAAFYEGPGDRPRRQAW
                     VDEFTSALRGEHRGAYVAFLGDEGEERVHDAYPAETWERLSEVKARYDPTNLFRLNQN
                     VPPAGATTT"
     gene            14744..15739
                     /locus_tag="VF097_11550"
     CDS             14744..15739
                     /locus_tag="VF097_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005312983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6263470.1"
                     /translation="MSDLASPRQQAPEDLETILEQHRRELTAHCYRMLGSPFEAEDAV
                     QETMLRAWRAFDRFEGRSSVRSWLYRIATNVCLDMLEGRGRRALPMDLGPAGSARMPL
                     PEPLPEVTWVQPIPDGRAVASEGDPAELAEQRDSIRLALVAALQHLPPRQRAVLILRE
                     VLRWKAAEVAELLETSVASVNSALQRARATLASAGEDAARPTETMDEERRELLSRYVD
                     AFERYDMDALTSLLHDDAKWSMPPYELWLQTHEDVVVWCLGPGIGCRGSRLVPVEANG
                     VPAFGQYKPSESGQGYEPWSLQVPEISGGRISGLTFFLDTATLFPLFGLPLRLEQ"
     gene            complement(15650..16060)
                     /locus_tag="VF097_11555"
     CDS             complement(15650..16060)
                     /locus_tag="VF097_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263471.1"
                     /translation="MSSTPGPRHLRGGRRSALTQALVVGIREPLEPGMIPELCERLDA
                     ILDERDAGLVLCAVVGPRRANAVTVEALARLRIVARRRGRRFRVVHPWPELRGLVALM
                     GLEEVLLLQPEGEAEEREQRRGVQEEGEAADPPP"
     gene            16113..17075
                     /locus_tag="VF097_11560"
     CDS             16113..17075
                     /locus_tag="VF097_11560"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX6263472.1"
                     /translation="MEITVGTFNLNNLFSRFNFRARVDAIPEEERDVTVSFEFTERGE
                     YWFRTFRGRLIDPMPEEQRRRLAARIKAMDLDVLAVQEVEDVEALRDFNRFHLEGMYP
                     HAVLVEGNDARFIDVAVLSKLPLGGATSWQHEVHPEDPSRPVFARDLLQVEVLNESRS
                     RRLLTVFNNHFTSKFISFGEDPVAAAAAKTARRRRQAETVERIVDRQLRPDSPFVIVG
                     DMNDTPDSEALAGLAQGSLGLVDGLADPEETRPARPDDPPPATKAWTHRFKQGGQPAR
                     YELFDQIWLSPSLGPKQTGAFIERRTLHGGDGSDHDPAWVVLSI"
BASE COUNT         2457 a         5967 c         6123 g         2576 t
ORIGIN      
        1 ccccgctgcc catccgggcc agcatggtcc cgacgccgag cagcacgccc aggcccatgg
       61 cgagagcccc gatgtggacg tcggccgcga tcttcagacc gaggaacagc acgaggccga
      121 ggttgatcgt ccacagcgcg gcgggctcgc cccaggccag gacgtgccgc cgctcgttcg
      181 tgcgcgccga cagcacgccg aggatcaggt tcgtcatcat gccgacgaag cctgcgtgca
      241 cgaagacggc cccgaaccag gaggggagcg agtcgaagtc gccgctgccc accgcgtaca
      301 ggaacaggcc gaggaacgcg atcagccagg ccgttccgaa gaacgcccaa cgaacgctgg
      361 cttcctgcgc cgccggcccg acggcgagcg ctcgccaggc gacccgtccg aggaacagca
      421 ccaagcccag caggagcagc aggccgaaca cggggagcag ctggtcgacg aggtccagca
      481 ggaaggcgag cggcacgagc gtcgccccga cgccccaggc cagcccctgg gccagcccag
      541 cgcgcgtcca ccgccgcatc ggatcctgag cgatcgtcca ttcgatgatc gcggccgcga
      601 gcaggaacag gtaggtgtcc atcatcccgg catggacccc aacgcgatcg ggatccacga
      661 tgggcaggaa ctcgccgatc acccgctcca gaccgagcag ggcgcccatc gtggccccga
      721 tcgcggcgac cagcagggcg gccgccacca ggacgtgcac cgtggtcacc acctcctggt
      781 gggggagctg ccggaggacg aagatcgcgg agatccacag cacgatcacg gcgaggcttc
      841 cgaagatggg gagcagggcg acgagcgggt cgtctccccg tgagaaggcc agccagaagt
      901 tggggatgta gccggcgaag accagcaccg cggtccagac gaggaagccg accctccgct
      961 cgtagtcccg cgagaccacc cggctgcccg tgaagaccca gatgccgatc ccgatcgccg
     1021 agagcgtgat ccatccgatg ctgcccgcgt gcaggtggat aagtgcctgc caccttggga
     1081 tgcccccgga cgtgagcgtg ttgtcgaacc cgaaccagat gttgatgagg aacaggagcg
     1141 cggcgcccgt gaacaggacg cggagatgcc cccacacggc ctcgctccga accggggtga
     1201 tgcccatctg tctcagctcc cttccgtcgg ttgggcggag aacacggtca cgggctcgga
     1261 cacgcccttg agggccacag atccgatctc gcggaacgag gcgggggcgt cgccggaggc
     1321 gtcgaccacc gagctcgtca cgaggacctc tcgtgggcgc gcatagtccg ccaggcgggc
     1381 cgccacgttc acggtccttc cgaagaagtc tgcgtcccgg gcgatcagcg gcccggcgtc
     1441 gatcccgagg cgggctgggg gtaggcccga ccgcgggatc cggtcgatca gccggagcgc
     1501 acaggtcacg gccgcccccg cttcggggaa gtggagcatg gcaccgtcgc ccagcatctt
     1561 gaccaggcga cccgggctcc ctccgatcgt tcgccggacc gcgtccagca tcgctcgggc
     1621 ttgctccgcg gccgcgtggt ctccgacccg atcggtcatc tgcgtgtagc ccgagaggtc
     1681 gacgaacgcg atcgcggggg gcctggggtt ccggcggaac gcgacgccgg cctcctccag
     1741 cgcgagctcg agatgctcga tcgacgcttg gaccacgacc tgctcgatga accggttcca
     1801 gaggatcccg atcagccggt ccccgatgac ctgggactcg ggcgcggggt ccgccgacgc
     1861 ttgcagcagc tcctggtgag agagtccgga ggcgaggagc ggcgcctcga caccggtcct
     1921 ccacaactcc cgtcccgcgt ccgccatgcg cctggtgctg tccaccacgc tgtggacgaa
     1981 ccggatcagc gacggctcgt cgagccccag ctcgatcatg cggtcgagca ggcggaggag
     2041 ctccgcgtcg tcctctcgaa cgggctcgtc gcccgtcaca ccgagggaga cgcgtacgtc
     2101 gtcgaagagg cgttcggaca tctggagctc ctcgaggagc tccgcgtacg tggtgggcag
     2161 catcgcgacg ggccgcctga acacgcggtc agcgaatgag agcgagaacc cctcccggcc
     2221 gctccgtcgc ccgatctgct ccagcgggat tccggaggcc tcgagctcca gggccagccg
     2281 gacgcgcggg accaaagcct cctcgaagcg gggctccgag gggaggatcc tcaggtcggc
     2341 cagccggcgg atctgctcgg ccgtcgtccc cgcccgcgcg gccagctcct ccgccgagag
     2401 cgtcatcgag aggtgatcgc cccctcgctg ctgaggagga gaaggatcga gacgacggcg
     2461 atccacagga acgcgatcgc gatgaagatg taggagaagg gggagatcag cccgatcgtt
     2521 agaacggctc cggaccaggc gagccagccg ggcagggccc cagcgcgtag cgcgacgagg
     2581 ctgctcgcgc cgaggagcgc cgccagagtg atggggacgg ctacgccgat gagcgatccg
     2641 tagatgtcgt agaggaccat ggcggtcgcg ggcgtgacct ggccctcgct gccgcgtgct
     2701 cccgccaccg acaggatcga gaaggcgatc cccgttccga cggcccccgc cacgccgccg
     2761 ccgaacgcga ccgtggacag gcggccggtg ccgccctcgg agaccctgag atggctccgg
     2821 acgctccccg agaaccagat caggaacacg gaggagagcg cgcctagata ggcgccgatc
     2881 tgtacgcgcc gcgcgttctc ctggaagaac gcggcgatct cgtcgccggc ggggagatag
     2941 ctgtactggc cgatcacgag ggccgctgcc agggccagcc ccacgaaagc cagcccggtc
     3001 cccggtgcga tccgggtcac ggtcgtcgtg ctgcttcggt cgagatccgt catccctcct
     3061 cctttcctgg gcgagccagg acgaggccgg ccgcggccag cagcgtcagg cctccgtagt
     3121 accaggcgat cggattcccc tccgccacca ggaccgaggt gatcgcagcg gtgattgccg
     3181 ggagcgtcca cagcatccgc cacacgggcc ggcccgccga acgcatcgac agggcgcccg
     3241 ccaaggccat cgtccccagt cctgccgcgg ccacccccgt caggcggagg agccgttcga
     3301 ggtagacggc cacggcgggt cggttggcga ggagctcctc ccaggagatc ccactgcgcc
     3361 ggaggtcgtc cgcatcgggc acggtgttca cgaccaggta ggtgcccgag agcagaaggg
     3421 cggcggagac caccccgaag agcgcccgac cggctcgttc gcgcgtgatc gcatctcggg
     3481 cgcgccgcct cccggttcgc cgcatcgtga ccatgcgggg tcaggctagg cccgcgaggc
     3541 ccgtctccgg aagcctgctt gcttcagctt tcggctgagg ttgtccctac ctcgggacgg
     3601 tggtcggcgg gatgttcgac gccgtgaccc agggattagg ctggtggcct caccccgcga
     3661 ggaggggagg gttcggggcc gtgagacggg tggcgatgtg ggggctcgcc ggcatcggcg
     3721 ccgcgtgcct ggtcatcacc gtggtcgtgg cgctctcttc gaacctggag ccgggcgtcg
     3781 ccgcgtggat cttcgtcggc ccgctgccga tcctcgggat gggcgcgttc ctctactggc
     3841 gccgcccgga tcaccgcacg tcccagcagt tcctggtgtg ggccgtcgtc accttctgcc
     3901 tcgccatgcc gatcgaagcg ttcacggtgg ctgccgtgga gggcggctcc ccgccctcgt
     3961 ggacctggct cgcggtgctc gtcttcatga ccgcatcggt tgtgggggcc gtgctggggg
     4021 cgacgctgat cgggctgctg ccggacggga ggttccggca tccctgggag cgccccatcc
     4081 ttctccccat gtggggcatg ctcgtggtgc cgctgctggc cttcctcacg aacgagcggg
     4141 tgccgctccc cggctacgtg ttcccgggga tcgacgacgc gagcagcccg ctcttcgtgc
     4201 cctcactcgc ggcgctcggt ccggtcgcga tgacgctcaa cgccctctat tccgtggtgt
     4261 tcgtggcggg catggggctc ctcgtcgtgc gctaccgaaa gggcggcgga cgggagcgaa
     4321 agcagctcag gtgggtgctg ctggcggccg tggtgaccgt cggcgccgcg atctggccgt
     4381 tcgtcctggg agagctcggg atcctccccg cggtgaccca tgggatctgg gctacggtcc
     4441 tgtggctccc gatcctgctg ctcccgatct cgatcgtggt ggcggtgttg gagccggcgt
     4501 ggctcgacgt cgacctcgcc atccgcaaga cattcgtgta cgggacgctc tccttcctca
     4561 tcctggccct ctacgtcggg gtggccaccg tttccggcgt cgccgcccgg gcggcgctcc
     4621 ccctggaggt ggcgatcctg gttgccgtcg gggtcgccgc gctcttccag ccggtccgtc
     4681 gctggctgca gtcggtcgcc gatcggtggg tcttcggtga gcccccggcc ggctacgagg
     4741 ccgtcaccga cttcaccacg gccgtcaagg aggctaccga tccgggtgac ctcgtcccca
     4801 ggatcgccga cacgacgcgc cgggccctgg ggctccggtg ggtcggcgcg accgtggagg
     4861 gcgagcggcc cgtcgtcctg ggggagcgca cggggcgtcc cgagctcgtg gcgccgatcg
     4921 agcaccaggg tgaccgcttc ggccgcatcg agtgcggccc caagtcgaag ggcgcgttca
     4981 acgaggccga cgtcgagctc gtgacggccc tagccagcca ggccgcgctc gccctgttca
     5041 acgcccgcct cgcgggaagg atcgtccata cgcaggaggc cgagcggcgc cgcatcgagc
     5101 ggaacatcca cgacggcgcg cagcaggagc tcgtggcgct ggtggccagg ctggggatgg
     5161 cccgcgccga cctgcggagg gatcgactct ccgaggagac gctcgttgag ctccagcggg
     5221 aggcgagccg catcctgtcc gacctgcggg agctggccca ggggatccat ccgtcggtgc
     5281 tcagcgatgg ggggctgctg gaggcgatcg aggatcggtg cggccgcctc cccatcgacg
     5341 tgtcgctgga ggccacgccc ggcctccggg cgaggcgctt ccccgatgag gtcgaggggg
     5401 ccgcctactt cttcgtcacc gagggggttg ccaacgtcct gaagcacgct caggcctccg
     5461 aggcgcgcat cgccgtccgc cacgaggacg gggtcctcgc cctcgacgtc agcgacgacg
     5521 gggtggggtt cgatccgtgg cgggttcgga ggacgggtct ctccggactc tcggaccgga
     5581 tcgccgccct cggggggacg ttcgccgtga ccagccgtcc gggagaggga gcctcgctcc
     5641 gtgccgtcct cccgatcgtg ccgccgggag cgtcggcgtg agcgatcggc tccgggtcgt
     5701 gatcgctgag gaccactacc tggtccgcga ggggacgcgc gggctgctcg agtcctcggg
     5761 cgaggtggag gtggcggccg cggtggagga tgcgccatcg ctcctcgacg ccgtggagcg
     5821 cctccatccc gacgtggtgc tcgtcgacat ccggatgccg cccacccacc agaccgaggg
     5881 tatcgacgcc gcgcgccgga tccgcgccgt ccacccggag gtcggcgtcg tggtgctctc
     5941 ccaacacgtg agctcgcact atgccttcga gctgttccgg gacgggacca gcggcttggc
     6001 ctacctcctg aaggaccgag tgggtgatct cgacgagctg ctggcggcgc ttcggtcggt
     6061 cgcggacggc ggatcggtga tcgatccgct ggtcgtggag cggctgctgg cgcagggcga
     6121 gcggctctcg gggacggggc tgtcggagct cagcccgcgg gagatggagg tcctggccaa
     6181 gatggccgag ggaaagtcga acgcggcgat cgcgggcgcg ctcttcgtct cgcagtcggc
     6241 ggtggagaag cacatcaact cgatcttcgc caagctgggg ctgagcgtgg acgagggaga
     6301 gacgcatcgg cgggtggcgg cggtgctcgc cttcctccgg agccaggatc agagccggga
     6361 gaccctggga tgagcgccct gctgcgcgtc gccggcctcc gcaagtcgta cggcgccatc
     6421 gaggccctgc ggggcgtgga cctggaggtc ctccccggcg agatcgtggc gctgctcggc
     6481 ccgaacgggg ccgggaagac gacgctggtc tccatcgtgg ccgggctgcg ccggcccgat
     6541 ggcgggacgg tggaggtcga tgggctcgat gccctgcgcc gctcggccga cgtgcgtcgg
     6601 cgaatcgggc tggctccgca ggagctgggg atctacccgg tggtgtcggt ccggagcaac
     6661 ctggcgctgt tcgggcggct ggccgggctg agcgggggcc agctcaggtc gcggatcgag
     6721 gacgtcgccg aggcgctgag gatcaccgac ctcctcgacc gccagggggg ctccctctcg
     6781 ggcgggcaga agcggagggt ccacaccgcg atcgccctgc tccatcgccc gcctctcctg
     6841 ctgctggacg aggccacgac cggggcggac gtcgagacgc gcggccacat cctcaacctc
     6901 gtgcggaggc tcgcgggcga tggatcggcg gtcctgtact cgacccacta cctccaggag
     6961 gtggaggacc tcggagccac cgtcgtcctc ctcgacggtg gccgggtgat cgcccgaggc
     7021 ggggcggccg agctcatcgc ggcccacgca acgaccgaac tggagatgac cttcgagggt
     7081 gacgtgcccg ctttgggcct caacggctcg gcgaccgtcg agggctcccg cctcaggctg
     7141 cccgcacagg atccggccgc caccctcgcc accctgattc cccgcgtccc gccggggacg
     7201 cttcgctcgg tggagatcgt ccgccccgac ctggaggccg tgtacctggc gctcacgggg
     7261 cgccgctacg aggaggccga cgaggagggg gagcgtgtcg ccgcgggcta gcgtcgccat
     7321 catgcgtcac gagttccgag tgctgatgac ggacccctcg acgttcgtct tcctcatcgt
     7381 catgcccctc gtgatggccg cgctgacgaa gcagctcttc cagggagcgc tggccgcgga
     7441 ggggttccgc ggcgcgaatg gatcggagtt cgccatcccc gggatggcgg tgagcttcgc
     7501 cgccttcggt gtcggctatg ccggcttcac cttcttccgg gatcacggat gggggacctg
     7561 ggaccgactc cgggccacgc ccgcgtcgtc actggacctg atcgccggga agacggttcc
     7621 ggtgctgctc gtcacgggcc ttcagctcgg gctgctgttc gcgctgggcg ggccgctgct
     7681 cggcctgcgc gtcacggggt cgggggtggc cctcgccgtg acgatcgtgg ctctggccct
     7741 ggccctggcc gccttcgggc tggcggtgac ggcggtggcc cgcacgatgc agcagctgaa
     7801 cgccatcggt tcggtcgggg gcttcgccat ggccatcatc ggcggggcct tcgtccctgt
     7861 ctcgtcgatg ccggactggg cgcaggccgt cgcgccggtg atgccgacgt attgggcgat
     7921 gaaggcgttc acctcggtga tcctcgaggg cgggggactc ggtgccgtcg ccgtgccggc
     7981 gatgatgatg ctggggttcg ccgtcgtgtt cgcgctgctg gtggctgcga agttccgcct
     8041 ggaggacacg aaggcgtact acggctgatc ctcctgatga ggctcagccg gcggtctcga
     8101 ccccttgcct ccgtccggcc gcgatcaagt cggcgatccg cgcggcatcg cggcccaccc
     8161 catggatctg ctccgacgac ttggcataga ggaacttgag gccgacgaag tacagcccgg
     8221 gttcagcgtc cacgactccg cgctcatgct tcggctcatc ggggcccaac acgggcaggt
     8281 cgatccagga gaagccggga tggaacccgg aacaccagat cacgtttgcc acatcgagac
     8341 ggcggccgtc ctcgagttga ggcaacccct cctcgacgcc ggtggtccgc ggcacccgct
     8401 caactcccgc cgccgccagg tccttcggct tgacccggat caggcggtcg cccttcttca
     8461 gcatcttggg acgggccctc cttcccatcg gggtgctggt cgtgagcacc cgatggaaga
     8521 tgacccgacc gacgaagggc atgagcaccc gcccgacgag ggtctcgggc cggaacggga
     8581 ggtggccggt gtcgggcccc gacagccaca cgcgccggct gcccgccagg tccatggcga
     8641 cctccgctcc cgagttcccc gcccccacca cgagagtgtc cccgtcccga agctgcttgg
     8701 ggttgcggta gtcggcgaca tgtagctgga ggatgcccgg atcgagctcg gatgcgaaat
     8761 ctggtacccg gggtcgctgc caggaggaca tggcgatgac cacgttctcg gtttcgaagg
     8821 accggtcgcc ggcagtcgcg acgaagcgat ccccgttccg ggtcaggtgg tccacgcgca
     8881 tgcccctcct caccggcagc tcgaaccgca gagcgtagct ctcgaggtag tcggcgaact
     8941 cgtccttgct cgggaacctc cagcgaggcc ccgggaaacg catcccgggg agtccgttaa
     9001 atcggttagg agtgaagagc cgcagcgagt cccagcgatt ccgccatgcg tcgccgacgc
     9061 gctctcgggc gtcgaggatc aggaacggga ccccgcgccg ggcgaggtgg tagccgacgg
     9121 agaggccggc ctggcctccg ccgatcacga tggtgtcgat ccgttcggag ctcatcgatg
     9181 tcgcctcgct catgcggtgg accggactcg aagggccccc gggggcgcca cggtcgccag
     9241 gaagctgtct cggatcgtct gctcgcgaac catggcctca gactaagagg ccctcgaccc
     9301 gccgggaatc gggagttctc cccaggcggt ctctacacgg gcacgaggcc gtgctgcatc
     9361 gcgaacatcg tggcggccgc acgggtcgag acgccggcct tccgataggc gttctggatg
     9421 tggcggtcgg ccgtcttgac cgagatgccg agcctccggg cgacctgctt cgtctggagg
     9481 ccgcgggcga gcaggccgag gacctccacc tcccgctcgg tcagcccggc ggggcgctcg
     9541 atcggcggag gtggctggcc cgccgcctcg acgaccgccg cgaccatctg cgggtcgagg
     9601 cggccggcgc gcgcctcctc gccgagcagc tccgccgctc gctcgggcgg gatcgcctcg
     9661 cggtgcggcc gcggctcgca catggcgtgg aaggcgtccg ccgcggcgag gaggcgcgcg
     9721 ccggccccca gcgtggaggc cgccacccgg cggtggtagc cctccccgtc gagccgctcg
     9781 tggtgggcgc aggcgatctc ggccagcgaa gcgaggagcg gcgagcggac cagcacgcgc
     9841 tccgagtggt aggggtggag gcgtacgtgc tcccactcgt cgacggaaag cgggcccggc
     9901 ttctgccaga tcctcgggtg gatcgccacc cttcccacgt cgtgcacgag cgccgcgcgc
     9961 cggaccagcg cgtgctcgtt cccctcgaag ccgcagatct tcgcggcgga ggcggcgaga
    10021 tcggccacgc cccgcgagtg ccccgagaga tgggcggaga cgagatcggc gaagttcccg
    10081 atcgccgcca gcgccccgtc cacctgcccg tcctccaggg tgagccgggg gtggggctcg
    10141 gcgtcgagcg tcgcctccca cgcggaggtg ggctcgtcgg ccgcgccgaa gatctccggc
    10201 gcgtgctcga cgaggcgacg ggcgatcgtc gggtcgaagg ccctccccgc tcgccggcgg
    10261 gtgctgtcga cggctcgctc gtcgccgccc accactcgct ggtaggccgc gtccctggcc
    10321 acgtgcacga tgcgcagcgg gagggggatc tcctcgcccc gtccgcgctt cagcaggccc
    10381 tttccgtccc accgctcggt gagatgggcg aacaggccgt ggacctcggc ggggaggccc
    10441 agccgcttcg ccatcatctc cgccacctcg cagagcgagg cgaagtgggt cttgcggaac
    10501 ctcgccgccg tcatcaatcg cccggcggct tgggtcagac ggaccggtgc ggacacctcg
    10561 ggatcgggga gggcgcggac cacgcccgcg agcgcctcgc tcgacgaccc gaactgcgcg
    10621 ggggtcacgt tctcggtcct gcggcccgcg aagatcttcg tcgcgacgtc cgcgtcggtg
    10681 atgcagccgg agtacatgag gaggctcacg tggtaggtcc gccatgcggt gtcgcggtcg
    10741 acgtcgagga gctcggccag ccgcatcgcc atgagcgtcc cgtggaggcc gtgctcgaag
    10801 gggaagccca tcccgaggtc cgtcgcgaga caggcggccg cgatgatctc ggcggcccga
    10861 accgactcgg gggcgactgc aggctgttgg gacacctcga ccagggtacg cgaggctcgg
    10921 ttccctcgcg cgaccgccgg gtggcgggag cgcccccctg atgcgaggct aacgggattc
    10981 cgagatgagg gggatgctga tggacgacta cccggtgcgg ttcgacgtgg actacccaga
    11041 ccgagccctg gaccgcctga ccaccttctt ccggctcgtc tgggcgatcc ccatcctgat
    11101 tctgctcgga accatcgcgg ggggaacctg gcagtggagc tacgaggaga gtcgatacgt
    11161 ggcggccggc gccggaggcc tcctcttctt cggacccctt ctgatgatcg tgttcaggca
    11221 gaagtacccg cgctggtggt tcgactggaa cctggcctgg cttcgcttct cgaaccgggt
    11281 ctccgcgtac ctggcgctgc tggacgatcg ctatccctcg tcggacgagg agcaggccgt
    11341 ccacctcgag atcccgtacc cggacgcgca ggaggacctg aaccagttcc tcccgctggt
    11401 gaagtggttc ctggcgatcc cgcactacat cgtgctggtg ttcctgtacg tcgggggagt
    11461 cttcgcggtg atcttcgcct ggttcgccat cctgttcacc gggcgctacc cgaggggtct
    11521 gttcgacttc gtggtgggcg tgttccgctg gcaaaaccgg gtggtgggct acgccttcgt
    11581 gctcgtgacc gatcggtacc cgccgttctc gctcgcgggc tgatccgcgg gccgtccgtg
    11641 gggccctcgc cgagagaggc tgtggtagcg tcggggaagt gattcggatg cgtgtcgacc
    11701 gacgctcgtc gacccacgtg ctgctcgtgc tcgcgctcgt cttcctcgcg atcgtcctgc
    11761 tgtggcacct cttcgggatg gaccactccg agaggatggg catcctcggg gggtgcctgt
    11821 tcctcctgac ggcggcgctg gtgctggtgg tgcccgccat ccggtggccc ctgccagcgc
    11881 ccacggaggc tcggcgagag gggccgccca cggccgtcct gcatccggtg tcgcgcccgc
    11941 ctccgatcga ggcctcgctg gcgagcgtcg tgctgatcaa ctgagtccac tccttcgcgc
    12001 tgtcctgttc ccgaacagcc gaaaggagag actcacatgt cgtatccaac gcggacgatc
    12061 agatggtggg ccggcatcgc cgtcgtgctg gtgaccgtgc tcctcgcgac ggcatgcgcc
    12121 gatacggaga ccggtgacgg tgccgagggg ggaaccgtcg acgtgaccct ccaggagtgg
    12181 tccgtcgttc cgggtcagga ctccgtggcg gccggacccg tgacgttcaa cgtcaccaac
    12241 gaggggccgg accatcctca cgagctcgtg gtgatcagga ccgacctcga ccccgctgag
    12301 ctacccacga cggacgaggg cggcgtggat gaggacgggg aggggatcga ggtcatcggc
    12361 gagatcgagg agttcccccc gggggagacg cacgatgcca cgttcgatct cgagcccggg
    12421 agctacgtgc tcgtctgcaa cgtcgtcgcg gtggccggcg agcacggcga gggccacgaa
    12481 gaggaggagg agatggaaga ggagggtgag cacgagggca tggaggagat ggagatccac
    12541 tacatgctgg gcatgcgcgc tcccttcacc gtgacctgag agcgaggaag gtcccggaca
    12601 agaggggtcc ttcgaccctc gtcccgggga ctccggacgc cgagcatccg actcgcaggc
    12661 cgcgtggatc gcggacgacc cgatgagaag ggagtcgaga tgcggatggg acggctgctc
    12721 tgggggctgg tggcgttctg gggcgcgatc ggggccgtcc tggccgtcgt cgctctgagc
    12781 cagccgccgg ccgaggagag cccgccggcc gcggcccctc ccgccctgcg cacggtcgtg
    12841 gtctccgaag cggcgcccgc gcagccctcg agccccgacg ggccggtgga ggtcgcgctg
    12901 ctgggcgggg acgcgccatc cgagccgggt atggcaacgg tcctcagcga cgagaactgc
    12961 gaaccggatg cgaacggcta ctcgcactgc ctcaacgagc tcgagatgga ggacggcgaa
    13021 cggatcgcgg tccgccacac gcaccgcatg gcggaggtgc cctgcctcgc cccgggcgag
    13081 cgggtgaacg tcaggttgtc gtagcgcccg ccggcgggac gttctggttc aggcgaaaga
    13141 ggttcgtcgg atcgtagcgg gccttgacct cgctcagccg ctcccacgtc tcggcggggt
    13201 aggcgtcgtg cacgcgctcc tcgccctcgt cccccaagaa ggccacgtac gcgccgcggt
    13261 gctcgccgcg gagcgccgac gtgaactcgt cgacccatgc ttggcgccgt gggcggtccc
    13321 ccgggccttc gtagaacgcc gcgaggttgc ccatgatgcg cgcgtcgcga tgggcgaacg
    13381 ccgtggcgtc ggccggcacc cgggccatcg cccccccgag cacgcgcagt tgcgcggccc
    13441 gcatcgcggc gtccgatgcc tcgaggcgct ccacgatcat ctcggccgca gccgcgtcga
    13501 cctcctggat cagcatcgtc tgagcgattg ccgtgggacg gtactcctcc tgctcgggag
    13561 ggaacatctc cgggtacggc atgggcctca gcaggtccgc gacgggctcg gcgagcgacc
    13621 ggaacggcgc gacggcccgc tcgccctctc cgacgtcccc tgcgtggacc atgagggcga
    13681 gcaccacgag ctcgccgtgc cgctcctcgg ggacgaaggg catcggggga gcgggcatca
    13741 cgttcgcgat cgtggacagc tcctcggggg cgcgttcggc ctccacgatg aatccggcca
    13801 cgacctccgc ggtggcgggg agcagcagca tcccgcccat caccctgtcg acggggtgga
    13861 ggcggaacag gaacctcgtg acgacgccga agttgccgcc cccaccgcgg atcgcccaga
    13921 agaggtccgg atgatgctcc tcgtccacgc ggagcagctg atcgtcggcc gtgacgatct
    13981 cggcggccag gaggctgtcg atcgtcatgc cgtaccggcg gaccaggagg ccgatgcccc
    14041 ctgccaacgt gatgccgccg atgcccaccg ttcccgtgtc gccgaagggc gtggccaggc
    14101 cgtagccccc cgcggccgcc gtgtactcgc cggcggtcag ccccgcctcg gcccaggcgg
    14161 tgcgtccctt gacgtcgatc tcgaggccct tcatggcgcg gagatcgagg acgatccctc
    14221 cctcggtcgc gctgtgtccg gcgccgctgt gccccccgct ccgcacggcc aggtccagtc
    14281 cggtctcccg ggccagcgcg atcacacgcg ccacgtcctc ggcgtccgtg ggccggacga
    14341 tcacggcggg tcgcttgtcc acgtcccccg ccacgaccgt gcgggcctcc tcgtagccct
    14401 cgtcctcggg ccccatcacc cgtccggtga agcggtcccg gaggtcgggg atcgcctgct
    14461 tcagcgtcat cgtgctccct tctctcgtgc tctctcctat gacggtcgcg ggcccgggaa
    14521 tgctaggagc cgaccgcggg aggcttcgaa gagcttccac tcgcccgttc gtcgcgtccc
    14581 tgatacgacg gttgggggca tcgaagctca tcggttgatc aagaacgcag gcctgatccc
    14641 tcctcgtccc ttgatgaaga acatcgtcgt ctcccggcag gcccccgatc cccgcctcgt
    14701 cgtcgatatg atgtggggac ccgcccaccg aggagcgaac cccatgagcg atctggcttc
    14761 accccgccag caggcccccg aggacctcga gacgatcctg gagcagcatc gccgcgagct
    14821 caccgcgcac tgctaccgga tgctcggctc cccgttcgag gccgaggacg ccgtgcagga
    14881 gaccatgctc cgggcgtgga gggcgttcga tcgcttcgag ggccgctcct cggtgcgctc
    14941 ctggctgtac cgcatcgcca ccaacgtgtg cctggacatg ctggagggga ggggacggag
    15001 ggccctcccg atggacctgg ggccggccgg gtcggcccgg atgccgctcc ccgaaccgct
    15061 ccccgaggtg acctgggtcc agcccatccc cgacgggcgc gccgtcgcct ccgagggcga
    15121 ccccgcagag ctcgccgagc agcgcgactc gatccggctg gcgctggtgg ccgccctcca
    15181 gcacctcccg ccgcgccagc gtgcggtcct gatcctgcgg gaggtcctgc ggtggaaggc
    15241 ggccgaggtg gccgagctgc tggagacctc ggtggcctcg gtgaacagcg ccctgcagcg
    15301 ggcccgggca acgctcgcct cggcggggga ggacgccgcg aggccgacgg agacgatgga
    15361 cgaagagcgc cgggaactgc tctcgcgcta cgtcgacgcc ttcgagcggt acgacatgga
    15421 tgcgctcacc tcgctcctgc acgacgacgc gaagtggtcg atgcccccgt acgagctctg
    15481 gctgcagacc cacgaggacg tcgtggtctg gtgcctcggc cccgggatcg ggtgccgggg
    15541 ctcccgcctc gtcccggtcg aggccaacgg ggtcccggcc ttcgggcagt acaagccctc
    15601 ggagtccggc cagggctacg agccgtggtc cctccaggtg cccgagatct caggggggag
    15661 gatcagcggc ctcaccttct tcctggacac cgcgacgctg ttcccgctct tcggcctccc
    15721 cctccggctg gagcagtaga acctcctcca ggcccatgag ggcgacgagg ccgcggagct
    15781 cgggccacgg gtgcacgacg cggaagcggc gtccccgcct gcgggccacg atccggagcc
    15841 gggccagggc ctcgacggtg acggcgttcg ccctccgtgg acccacgacc gcgcacagga
    15901 cgaggccggc gtcccgctca tcgaggatcg cgtcgaggcg ctcgcagagc tccgggatca
    15961 tccccggctc gagcggctcc cggatcccca cgaccagcgc ctgggtcagc gccgacctcc
    16021 ttcctcctct caagtgacgg gggccgggcg tggaactcat cggagggcga agatgaccct
    16081 gacgtcgctc gtggagcccg ccaggaggga tcatggagat caccgtcggg accttcaacc
    16141 tcaacaacct gttctcgcgt ttcaacttcc gggcccgcgt ggacgcgatc ccggaggagg
    16201 agcgcgacgt caccgtcagc ttcgagttca ccgaacgggg ggagtactgg ttccgaacct
    16261 tccgggggcg gctgatcgac ccgatgccgg aggagcagcg ccggcgcctc gctgcgcgca
    16321 tcaaggcgat ggacctcgac gtgctcgccg tccaggaggt cgaggacgtg gaggccctcc
    16381 gggacttcaa ccggttccac ctcgagggca tgtaccccca cgcggtgctg gtcgagggga
    16441 acgacgcccg cttcatcgac gtggcggtcc tctcgaagct gccgctgggc ggggccacgt
    16501 cgtggcagca cgaagtgcat ccggaggatc cctcgcgccc cgtcttcgcc cgtgacctgc
    16561 tgcaggtcga ggttctgaac gagtcgcgct ccaggcggct gctgacggtc ttcaacaacc
    16621 acttcacgag caagttcatc tcgttcgggg aggacccggt cgccgccgcc gccgcgaaga
    16681 ccgcgcggcg gcggcggcaa gccgagaccg tggagcgcat cgtggatcgg cagctccgcc
    16741 ccgactcccc gttcgtgatc gtgggcgaca tgaacgacac gcccgactcc gaggcgctgg
    16801 ccggcctggc gcaggggtcg cttgggctcg tggacggcct ggccgatccg gaggagaccc
    16861 gtcctgccag gcccgacgac ccgccgccgg ccacgaaggc gtggacccat cgcttcaagc
    16921 agggcgggca gccggcccgc tacgagctct tcgatcagat ctggctgagc cccagcctcg
    16981 gtccgaagca gaccggcgcg ttcatcgagc gccgcacgct gcacggcggc gacgggagcg
    17041 accacgaccc ggcgtgggtc gtgctctcga tctagtcgcg gctggggacc tcctggagga
    17101 gccagaggtt gccgtcgggg tcc
//
LOCUS       DASUTZ010000269         3794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1141362, whole genome shotgun sequence.
ACCESSION   DASUTZ010000269 DASUTZ010000000
VERSION     DASUTZ010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141362"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1268)
                     /locus_tag="VF097_11565"
     CDS             complement(60..1268)
                     /locus_tag="VF097_11565"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6263473.1"
                     /translation="MRLLRIRDLRIVLLAYGLSAFGDYLALIALTLRIKDLTESGLAV
                     SALLVAGILPIVVLGPLAGHVVDRLEPTRVLTFGALIQAGLAFALVFAEEPWLIVTLF
                     FLLSTGFALSQPGIFAMTPWIVGEDHTTEANAYLEVSRWAGAAIGPIVAGVISHTWGS
                     DAALLLNALTFLAVGLAMPLLRVRRATDATPPATPERARDGVVFIGRDPILRVVVVAI
                     TLMVVFSAIDNVAEVFFAKDVLEAGDLGYSGLVTAWTLGMVIGATGIGRRLTTDRLGP
                     AVVVAGIVGGGAIALAAGIPMLPLALGAFVVGGAANGVELVGLRSLIHARVPDRLRGR
                     AFAAYYAAVNAAQITALGVGGGLVGAIGPRASLLVGGMGTLLVGAAGFFFLLRARAAV
                     ARAGYSTSVS"
     gene            1400..3286
                     /gene="ftsH"
                     /locus_tag="VF097_11570"
     CDS             1400..3286
                     /gene="ftsH"
                     /locus_tag="VF097_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX6263474.1"
                     /translation="MKPRRPFRGPVLYLILVLGALWLFLWMGDRGPEAAELSVTELES
                     LAAEGAVVSAEFMERDQRVEGELSSGERYTAPYLGEYGDDLLETLQRSGVEVSANPQP
                     DPVFVRILNFLPLLLIFGLIVWIFVQQSGGGGGSRVMSFGRAKARQVTKDMPKTTFED
                     VAGVEEAIEELQEVKEFLENPKKFEAMGARIPKGVLLFGPPGTGKTLLARAVAGEAGV
                     PFFSISGSDFVEMFVGVGAARVRDLFQQAKDAAPAIVFIDEIDAVGRHRGAGLGGGHD
                     EREQTLNQLLVEMDGFDARTGVILMAGTNRPDILDPALLRPGRFDRQIVVDYPDLNGR
                     KGILAVHARGKPLATGVSLDDIAKQTPGFTGADLANLMNEAALLAARRGEKHISKAEL
                     EEAIDRVLMGPEKKTRLISDKEKRVIAYHEAGHALVGFAHPDVDPVHKVTVIPRGRAG
                     GYTLVLPTEDRMLNTRSELLGQLAYGLGGRVAEQLVFGDITTGANDDLQKASKLARRM
                     VTEFGMSDLLGPITLGQRQHEVFLGKDIAHTPDYSDQVAFEIDTEVRRLIDEAHDEAL
                     ATLKRNRKKLDGLAEALMERETLDKDEVAEILKGARGTRKNGERRGAGIAAFRRSPGS
                     GRRS"
     gene            3286..>3794
                     /gene="folE"
                     /locus_tag="VF097_11575"
     CDS             3286..>3794
                     /gene="folE"
                     /locus_tag="VF097_11575"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX6263475.1"
                     /translation="MPEFDERKAEDAVERLLEAIGEDPGRDGLKETPRRVAAMYREIF
                     AGMRQDPEAVLDVTFEAGHSEMVMVRDMPLYSMCEHHLVPWVGKAHVAYIPGKDGRIT
                     GLSKLARLVEVFAKRPQVQERLTTQIADTIEKVLRPRGVFVVIEAEHLCMTMRGVRAP
                     GSVTVTSAVR"
BASE COUNT          623 a         1300 c         1387 g          484 t
ORIGIN      
        1 ctcgactacg cggagcgcta ccgcaacctc cccttcgtgg ggacgctgaa gccgcaggtc
       61 tacgagacgc tcgtcgagta gccggcgcgg gccaccgccg cccgggcccg cagcaggaag
      121 aagaaccccg ccgcccccac cagcagggtc cccatgccgc ccacgaggag cgaggcccga
      181 ggcccgatcg ctccgacgag cccgcccccc acccccagcg ccgtgatctg cgccgcgttc
      241 acggcagcgt agtaggcggc gaaggccctc ccccggaggc gatccgggac ccgggcgtgg
      301 atcaggctcc ggaggcccac cagctcgacc ccgttcgccg cgcctccgac gacgaaggcg
      361 cccagggcga gcggcagcat cgggatcccg gcggccagcg cgatcgcccc gccccccacg
      421 atgccggcca cgacgacggc cgggcccagc cggtcggtgg tcagtcgcct cccgatcccg
      481 gtggccccga tcaccatccc cagggtccac gccgtcacca ggccgctgta cccgaggtcc
      541 ccggcctcca gcacgtcctt ggcgaagaag acctcggcca cgttgtcgat cgccgagaag
      601 acgaccatca gcgtgatcgc cacgaccacc acgcggagga tcgggtcgcg gccgatgaag
      661 accaccccgt cgcgagcccg ctcgggcgtg gccggcggcg tcgcgtcggt ggccctccgg
      721 acccggagga ggggcatggc caggcccacc gccaggaacg tcagcgcgtt caggaggagc
      781 gctgcgtccg agccccaggt atgcgagatg accccggcca cgatcgggcc gatcgcggct
      841 ccggcccacc tcgagacctc caggtacgcg ttggcctcgg tggtgtggtc ctcgcccacg
      901 atccacggcg tcatggcgaa gatcccgggt tgactgagag cgaagcccgt gctcagcagg
      961 aagaagaggg tgacgatcag ccacggctcc tcggcgaaga ccagggcgaa cgccagcccc
     1021 gcctggatca gcgcgccgaa cgtgaggacg cgggtcggct cgagccggtc cacgacgtgc
     1081 ccggccaggg gacccagcac gacgatcggg aggatccccg ccaccaggag cgccgacacc
     1141 gccagcccgg actcggtgag gtccttgatg cgcagcgtaa gggcgatgag cgccaggtag
     1201 tccccgaacg ccgagagtcc gtaggcgagc aggacgatcc ggaggtcccg gatccgcagg
     1261 aggcgcatgg gcgatttgta ccgccgttga ggcggtccgt ccagccccgg gggccgactc
     1321 cgttcgagag gccctgtgct accatcgccg caccgaccgg ccccgcccct ggatggaacg
     1381 aaggagaccc ccgaccgaca tgaagccccg tcgccccttc cgagggccgg tgctctacct
     1441 gatcctggtc ctgggcgcgc tgtggctctt cctctggatg ggggaccggg gccccgaggc
     1501 cgccgagctc tccgtcaccg agctggagag cctggccgcc gagggggccg tcgtctccgc
     1561 ggagttcatg gagcgcgacc agcgggtcga gggcgagctg tcgagcggcg agcggtacac
     1621 ggctccctac ctgggcgagt acggcgacga tctgctggag accctccagc ggagcggggt
     1681 cgaggtctcc gcgaatcccc agccagaccc ggtgttcgtc cgcatcctga acttccttcc
     1741 actcctcctg atcttcgggc tgatcgtctg gatcttcgtc cagcagagcg gcggcggggg
     1801 tggctcccgg gtgatgtcgt tcgggagggc caaggcccgc caggtcacga aggacatgcc
     1861 gaagacgacc ttcgaggacg tggccggcgt ggaggaggcc atcgaggagc tccaggaggt
     1921 gaaggagttc ctggagaacc ccaagaagtt cgaggcgatg ggcgcgagga ttcccaaggg
     1981 ggtcctcctg ttcgggccgc cggggaccgg gaagaccctg ctggcccggg ccgtggcggg
     2041 ggaggccggc gtgccgttct tctccatctc ggggtcggac ttcgtggaga tgttcgtcgg
     2101 cgtcggggcc gcgcgcgtcc gcgacctctt ccagcaggcg aaggacgccg ccccggccat
     2161 cgtgttcatc gacgagatcg acgccgttgg gcgccaccgt ggcgcggggc tcgggggcgg
     2221 gcacgacgag cgcgagcaga cgctgaacca gctgctcgtc gagatggacg ggttcgacgc
     2281 gcggaccggc gtgatcctga tggcgggcac gaaccgtcca gacatcctcg acccggcgct
     2341 gctacggccc ggacgcttcg accgccagat cgtcgtggac tacccggacc tcaacggacg
     2401 gaagggcatc ctggcggtcc acgcccgggg caagcccctg gccacgggcg tgagcctcga
     2461 cgacatcgcg aaacagacgc ccgggttcac cggggccgat ctggccaacc tcatgaacga
     2521 ggcggcgctc ctcgcagcgc gtaggggcga gaagcacatc tccaaggccg agctggagga
     2581 ggcgatcgac cgggtgctga tgggcccgga gaagaagacc cggctcatct ccgacaagga
     2641 gaagcgggtg atcgcctacc acgaggcggg ccatgcgctc gtggggttcg cccaccccga
     2701 cgtcgatccg gtgcacaagg tcaccgtgat cccgcggggg cgggccggcg ggtacacgct
     2761 cgtcctcccc accgaagacc ggatgctcaa cacgcggtcg gagctgctgg gccagctcgc
     2821 gtacgggctg ggcggccggg tggccgagca gctggtcttc ggggacatca cgacgggagc
     2881 caacgacgac ctccagaagg cctccaagct ggcgcgacgg atggtgaccg agttcgggat
     2941 gtcggacctg ctcgggccga tcacgctggg tcagcgccag cacgaggtgt tcctgggcaa
     3001 ggacatcgcc cacacccccg actactcgga ccaggtcgct ttcgagatcg acaccgaggt
     3061 gcggcgcctg atcgacgagg cgcacgacga ggcgctggcc acgctcaagc ggaaccgcaa
     3121 gaagctcgac gggctggcgg aggccttgat ggagcgggag acgctggaca aggacgaggt
     3181 ggccgagatc ctgaagggcg cacgcgggac ccggaagaac ggcgagcggc ggggcgccgg
     3241 gatcgccgcg ttccggagga gccccggcag cggccgccgg agctgatgcc cgagttcgac
     3301 gagcggaagg ccgaggatgc cgtcgagcgc ctgctcgagg cgatcggcga ggaccccggc
     3361 cgcgacggcc tcaaggagac cccccgccgc gtcgcggcga tgtaccgcga gatcttcgcg
     3421 gggatgcgcc aggaccccga ggcggttctc gacgtcacgt tcgaggccgg ccacagcgag
     3481 atggtcatgg tgcgggacat gccgctctac tcgatgtgcg agcaccacct cgtgccgtgg
     3541 gtggggaagg cgcacgtggc gtacatcccg ggcaaggacg ggcggatcac ggggctgtcg
     3601 aagctggcca ggctggtcga ggtgttcgcg aagcggccgc aggttcagga gcggctgacg
     3661 acgcagatcg cggacacgat cgagaaggtg cttcggcccc gcggggtgtt cgtggtgatc
     3721 gaggccgagc acctctgcat gacgatgcgg ggggttcggg cgccgggttc ggtgacggtg
     3781 acgtccgccg tgcg
//
LOCUS       DASUTZ010000270         6040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1919920, whole genome shotgun sequence.
ACCESSION   DASUTZ010000270 DASUTZ010000000
VERSION     DASUTZ010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6040
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1919920"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VF097_11580"
     CDS             <1..753
                     /locus_tag="VF097_11580"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX6263476.1"
                     /translation="NSSAFRQDPGVPLVIPEINAHALRDHDGLIANPNCTATTALMAV
                     APLHRAAGVRAMVTSAYQSVSGTGRRAMQELLDETRKTIDQVEGLRGQEELDGPGPEV
                     YPHLIAFNVFPQCEIFPAGADTSTEEAKMEAESRKILEHPDLIAHATSVRVPVVVGHA
                     VSVALSLERPMAPEEARELLAAFPGVSVVDDPERAGYPTAQAAAGIDDTLVGRVRANP
                     AMEHGLSLFVCQDNLRKGAALNNVHIAEALGS"
     gene            750..1193
                     /locus_tag="VF097_11585"
     CDS             750..1193
                     /locus_tag="VF097_11585"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX6263477.1"
                     /translation="MTDQRTEPVYEIDLRDLADEGAGPVVIPDPDPDPAVETVTLPAE
                     GEALAGDPPPGDVPALLEMLAHPRPDRRRIACERLGYQRARAAVEPLIERLQDPERGV
                     RIKAGDALALIGDERAVQPLERARAADPDPGVTQAMERALRTLAR"
     gene            complement(1195..2310)
                     /locus_tag="VF097_11590"
     CDS             complement(1195..2310)
                     /locus_tag="VF097_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEX6263478.1"
                     /translation="MFPLSKGRVARQAHVGLPDGTYEEEHGREAFDGRASHLYRSHPP
                     TAWVRLDGPLRPRAYDLSGLKTPDQADADAGWQRVLFNEDVAIYVSRPTGAPSWYLRD
                     ADGDLCYFVHRGTGTLETDYGPLGFRDGDYLIVPKGTTHRVIPEGEDTYLYVIEAAGE
                     YHLPDKGILGKHALFDPGVIETPEPEPREEEGEFEVRVKRDDSFTTLVYPFHPLDVVG
                     WQGDLCPLRVNVEDFRPVVSPRYHLPPSVHVTWEADGFEIGTFAPRPTETGDPTALRV
                     PFFHSNIDRDEVLFYHRGQFFSRAGIGEAFITHHPQGLHHGPQAAAMEAGKTKEFADE
                     YAIMVETLRPLQRDEAIAAVEDESYATSWARGLGLIE"
     gene            complement(2453..3280)
                     /gene="proC"
                     /locus_tag="VF097_11595"
     CDS             complement(2453..3280)
                     /gene="proC"
                     /locus_tag="VF097_11595"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009297068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX6263479.1"
                     /translation="MTERRVAILGGGKMGEALLSGLLRSGRSPEDIMVTVRREERAAE
                     LAEEYGVQTALDNEEAVGWAKVLVLSVKPQDMEALLEQIAPAVTEDHVIVSVAAGIRC
                     ALIERMTPGDVPVVRVMSNVPVHVDEAMSAISPGAHAEDKDLAIAEELLASVGKVIRL
                     PEKHQDAVTATSGSGPAYFALLAEAMIDACILLGLSRDVATELIVQTMIGSAKMLRDM
                     NSHPVELREMVTSPGGTTIAAIRELENAGVRAAFLNAIEAARQRSVELAKGEDAEEV"
     gene            complement(3282..3821)
                     /locus_tag="VF097_11600"
     CDS             complement(3282..3821)
                     /locus_tag="VF097_11600"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEX6263480.1"
                     /translation="MAGRALIVTDIQNGFTRIGNLASERMTALIPTIVRIAEEERAAG
                     TPIIFTKDTHVENDKEFEVFPPHCIEGTEEHDLVEELLPFESDAEAVIEKHRYSAFFQ
                     TELDKVLERLAPDEVHIVGVCSDICVLHTTADLRNHDYPVVIRREGVETFDGPGHDAD
                     EMNRFALTHAETILGAKVV"
     gene            complement(4240..4713)
                     /locus_tag="VF097_11605"
     CDS             complement(4240..4713)
                     /locus_tag="VF097_11605"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEX6263481.1"
                     /translation="MSDEAVRILAFLRERAQEEELTLLASSDRAWDVLLPSYWKETLA
                     VSLELGDWNLRGESFFMRGPEENRAETFHLLLQRNRRPGPWRFAANEGGDISLLASIP
                     RAAVTEEELDRLLGSLVQITDETFVPAMKLGYERGLAEQVERGGPGLDQPPPWAR"
     gene            complement(4710..5972)
                     /locus_tag="VF097_11610"
     CDS             complement(4710..5972)
                     /locus_tag="VF097_11610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6263482.1"
                     /translation="MRAVGEARARVAMISLHTSPLDPPGTGDSGGMNVHVRETMHRLA
                     DRDVAVDVYTRCAGRGVPEVHELTPLTRVIQVQAGPCAAVHKRDLPELVPDFVRSFLG
                     RDLPPYDVIHAHYWLSGQAARAARSTWGAPLVASFHTLGRVKNASLAPGEPPEPETRI
                     RGEEALIRSADRVVAPTEIEARDLVNRYRARPGAIRVVHPGVDRFLFRPRPKDASRMA
                     WGLEGSRVALFVGRLQPHKGPDVAIRTVAEAMRADPDATRDLVLVLAGGSSGGWGPAD
                     LQALARAEGIADRVRFVDSVIHEELPSLYASADVLLVPSRTESFGLAALEAQACGVPV
                     VAASVGGLRLVVGDGESGFLVPGHDPRDHAKRVLRILRSPKLVMRMSAAAEHRAERFP
                     WDRTVDDLLDVYGELAPVLADPDAAVAP"
BASE COUNT          863 a         2228 c         2073 g          876 t
ORIGIN      
        1 aactcctcgg cgttccggca ggacccgggc gtcccgctcg tgatccccga gatcaacgcc
       61 cacgcgctcc gggaccacga cggcctgatc gccaacccga actgcaccgc gacgaccgcg
      121 ctgatggccg tggccccgct ccaccgggcc gccggcgtcc gggccatggt gacctcggcc
      181 tatcagtcgg tctcgggcac cggccgacgg gcgatgcagg agctgctcga cgagacccgc
      241 aagacgatcg accaggtgga ggggctgcgg ggccaggagg agctcgacgg gcccgggccc
      301 gaggtctacc cgcacttgat cgccttcaac gtgttcccgc agtgcgagat cttccccgcg
      361 ggggccgaca cctccaccga ggaggctaag atggaggcgg agagccgcaa gatcctcgag
      421 cacccggacc tgatcgcgca cgctacgtcg gtacgcgtgc cggtcgtggt cggccacgcg
      481 gtctcggtgg cgctctcgtt ggagaggccg atggctcccg aagaggcccg cgagctgctg
      541 gccgccttcc cgggggttag cgtcgtggac gaccccgagc gtgccgggta cccgaccgcg
      601 caggcggccg ccgggatcga cgacaccctc gtggggcggg tccgcgccaa cccggccatg
      661 gagcacggcc tctcgctgtt cgtctgccag gacaacctcc gcaagggcgc cgccctcaac
      721 aacgtgcaca tcgccgaagc cctggggtcg tgaccgacca gcgcaccgag cccgtctacg
      781 agatcgacct gcgggacctg gccgacgagg gcgccggccc cgtcgtgatc ccagacccag
      841 atccggaccc ggccgtcgaa acggtgacgc tgccggccga gggggaggcg ctcgcaggcg
      901 atcctccgcc cggcgacgtc cccgccctcc tcgagatgct cgcccacccc cgtcccgacc
      961 ggcggaggat cgcctgcgag cgcctcggat accagcgggc ccgggccgcc gtggagcccc
     1021 tcatcgagcg cctccaggac cccgagcgag gcgtccggat caaggccggg gacgcgctgg
     1081 cgctcatcgg ggacgagcga gcggtccagc ccctggagcg agcccgagcc gccgaccccg
     1141 acccgggggt gacccaggcg atggagcggg ccctccggac gctcgcccgc tgagctactc
     1201 gatcaggccg agcccccggg cccaggacgt ggcgtagctc tcgtcctcga ccgcggcgat
     1261 cgcctcgtcg cgctgcagcg gccgcagcgt ctcgaccatg atcgcgtact cgtcggcgaa
     1321 ctccttcgtc ttgccggcct ccatcgccgc cgcctgcggg ccgtgatgca ggccctgcgg
     1381 gtggtgggtg atgaacgcct cgccgatccc cgcccgcgag aagaactgcc cgcggtggta
     1441 gaagagcacc tcgtcccggt cgatgttcga gtggaagaag ggcacccgca gcgccgtcgg
     1501 atcgcctgtc tcggtcggcc tgggcgcgaa cgtcccgatc tcgaacccgt ccgcctccca
     1561 cgtgacgtgg accgacggtg gcaggtggta ccgaggcgag accaccggcc ggaagtcctc
     1621 cacgttgacc cgcagcgggc agaggtcgcc ctgccacccc acgacgtcga gcgggtggaa
     1681 cggatagacg agcgtggtga acgagtcgtc gcgcttcacc cggacctcga actcgccctc
     1741 ctcctcgcgg ggctcgggct ccggcgtctc gatcacgccg gggtcgaaca gcgcgtgctt
     1801 ccccaggatc cccttgtccg ggaggtggta ctcgccggcg gcctcgatca cgtagaggta
     1861 ggtgtcctcc ccctcgggga tcacgcggtg cgtcgtcccc ttggggacga tgaggtagtc
     1921 gccatcacgg aaccccaggg gcccgtagtc ggtctccagc gtcccggtcc cccggtggac
     1981 gaagtagcag aggtcgccat cggcgtcccg caggtaccac gacggcgcgc cggtgggccg
     2041 cgatacgtag atcgcgacgt cctcgttgaa gaggacgcgc tgccagccgg cgtctgcgtc
     2101 ggcctgatcc ggcgtcttca ggccgctgag gtcgtaggcg cggggccgca gcggcccgtc
     2161 caacctgacc cacgcggtgg gcgggtgcga tcggtacagg tgggaggccc ggccgtcgaa
     2221 cgcctcgcgg ccgtgctcct cctcgtaggt gccgtccggg aggcccacgt gcgcctgcct
     2281 ggccacgcgg cccttcgaga gtgggaacat cgaaccctcc tcagctcttg gcggccttcc
     2341 gggcctcgcc cttcgcctgc gccctccgtc ggtcgcgctc ccgtttcttc cgctcgcggt
     2401 cgtgcgccca tcgcaccttc ctgcggtctc ccctgcccat ccgacctcct cctcagacct
     2461 cttcggcgtc ctcgcccttg gccagctcca cgctgcgctg gcgggccgcc tcgatcgcgt
     2521 tgaggaacgc ggcccgcacg cccgcgttct ccagctcgcg gatggccgcg atcgtcgtcc
     2581 cgcccggcga ggtcaccatc tcccgcagct ccaccgggtg cgagttcatg tccctcagca
     2641 tcttcgccga cccgatcatc gtctgcacga tcagctcggt ggccacgtcc cgcgagagcc
     2701 ccaaaaggat gcaggcgtcg atcatcgcct cggccaggag cgcgaagtag gcggggcccg
     2761 agccggacgt ggcggtcacc gcgtcctggt gcttctccgg gagacggatc accttgccca
     2821 ccgaggccag cagctcctcg gcgatggcca ggtccttgtc ctccgcgtga gctccggggc
     2881 tgatcgccga catcgcctcg tccacgtgaa cggggacgtt ggacatcacc cggaccaccg
     2941 gcacgtcccc gggcgtcatc cgctcgatca gcgcgcagcg gatcccggcc gccacgctca
     3001 cgatcacgtg gtcctcggtg accgcagggg cgatctgctc gagcagggcc tccatgtcct
     3061 gcggcttcac cgagaggacg agcaccttcg cccaccccac ggcctcctcg ttgtcgagcg
     3121 cggtctggac gccgtactcc tcggcgagct cggcggcccg ctcctcccgg cggacggtca
     3181 ccatgatgtc ctcggggctg cggcccgacc ggaggagccc cgagaggagc gcctccccca
     3241 tcttcccgcc gcccaggatc gccacccgtc gctcggtcat ctcacaccac cttcgcgccc
     3301 aggatcgtct cggcgtgcgt cagtgcgaag cggttcatct cgtcggcgtc gtggcctgga
     3361 ccgtcgaacg tctccacgcc ttccctgcgg atgacgacgg ggtagtcgtg gttgcggagg
     3421 tccgcggtcg tgtgcaggac gcagatgtcg ctgcacacgc ccacgatgtg gacctcgtcg
     3481 ggagccaggc gctccaggac cttgtccagc tccgtctgga agaaggccga atagcgatgc
     3541 ttctcgatca cggcctcagc gtcgctctcg aagggcagca gctcctcgac caggtcgtgc
     3601 tcctcggtcc cctcgatgca gtgagggggg aacacctcga actccttgtc gttctcgacg
     3661 tgagtgtcct tcgtgaagat gatcggggtc cccgcggcgc gctcctcctc cgcgatccgg
     3721 acgatcgtgg ggatcagggc ggtcatccgc tcgctggcga gattgccgat gcgtgtgaaa
     3781 ccgttctgga tgtcggtgac gatgagcgcg cggcctgcca tcggaacccc cctgggtcga
     3841 gatggccaca gtataggaaa agcccgaggg acctccggct cgccgtcccg accctcgagc
     3901 cgaagctcga tgatcgcgac agccaccttc agcccctcgg gcttagcgct ccgagcaacc
     3961 tacccgatcg ctccggtacc cctcccggta gcccctttcg ttgggccttg gggccttccg
     4021 agcctcccca cccctacagc tagttggcct tcccaaccgc aggagcaggt gcgcccagtt
     4081 agccttacag ctttcccagg gggccccgct tggagcccga tccgagcggt tcagctgctc
     4141 ggagctagcc ggcaatctag cgacggcgag gaagcccgtc aaggtgccaa aaccccaggt
     4201 cgccaccggt ttccgcaggt cagcccggca gaaaaaaaat cagcgggccc acggaggagg
     4261 ctggtcgagg ccgggcccgc cgcgctccac ctgctccgca aggccgcgct cgtaccccag
     4321 cttcatcgcg gggacgaagg tctcgtcggt gatctggacc agcgagccga gcagccggtc
     4381 gagctcctcc tccgtgacgg cggctcgggg gatcgaggcg aggaggctga tgtcgccccc
     4441 ctcgttcgcc gcgaaccgcc atgggcccgg ccggcggttc cgctgcagca gcaggtggaa
     4501 ggtctccgcc cggttctcct cgggccctcg catgaagaac gactcacccc gcaggttcca
     4561 gtcgcccagc tccaggctca ccgccagggt ctccttccag tacgagggaa gcagaacgtc
     4621 ccaggctcga tcgctggacg cgaggagcgt cagctcctcc tcctgggctc gctcccggag
     4681 gaaggccagg atccgaacgg cctcgtcgct cacggcgcca ccgccgcgtc cgggtcggcg
     4741 aggacgggcg cgagctcccc gtagacgtcg aggaggtcgt ccacggtgcg atcccagggg
     4801 aaccgttcgg cgcggtgctc agccgcggcc gacatgcgca tcacgagctt gggcgagcgg
     4861 aggatccgca gcacgcgctt cgcgtggtcg cgaggatcgt gaccgggcac caggaagccg
     4921 ctctcgccgt cgcccacgac gaggcggagg cctccgacgg aggccgcgac gacgggaaca
     4981 ccgcacgcct gggcctcgag cgcggcgagg ccgaacgact cggtgcgcga ggggacgagc
     5041 aggacgtcgg ccgacgcgta cagcgagggc agctcctcgt ggatcacgct gtccacgaag
     5101 cggacccggt ccgcgatccc ctcggcgcgg gccagcgcct ggaggtcggc cgggccccag
     5161 ccgccgctcg agccgccggc caggacgagc accaggtcgc gtgtcgcgtc gggatcggct
     5221 cgcatcgcct cggccaccgt gcggatcgcc acgtcgggcc ccttgtgcgg ctggagacgg
     5281 cccacgaaga gcgccacgcg gctgccctcg aggccccagg ccatcctgga cgcgtccttg
     5341 ggccgaggac ggaacaggaa gcggtccacc cccggatgca cgacgcggat cgcgccgggg
     5401 cgggcccggt accgattcac gaggtcgcgg gcctcgatct cggtgggggc caccacgcga
     5461 tcggccgagc ggatcagggc ctcctcgccg cgtatccggg tctcgggctc ggggggctcc
     5521 cccggggcca gcgaggcgtt cttgacgcgc cccagggtgt ggaacgacgc caccagcggc
     5581 gccccccagg tcgagcgggc ggcgcgggcg gcctggcccg acagccagta gtgggcgtgg
     5641 atcacgtcgt agggggggag gtcgcggccc aggaacgagc ggacgaagtc ggggacgagc
     5701 tcggggaggt cgcgcttgtg caccgcggcg cagggtccgg cctggacctg gatcacgcgg
     5761 gtgagcggcg tcagctcgtg cacctcgggg accccccgtc cggcgcagcg cgtgtagacg
     5821 tccaccgcga catcccgatc ggccaggcgg tgcatcgtct cgcggacgtg gacgttcatg
     5881 ccgcccgagt cgcccgtgcc tggcggatcc agcggtgacg tgtgcaggga gatcatcgcg
     5941 acccgagctc gggcctcccc gacggctctc atccggtcaa tcctaggcca gaggtcgggt
     6001 tccgacacgg ggcggccgcc gcgcgcgttc gcgcgcggcg
//
LOCUS       DASUTZ010000271        30285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1661979, whole genome shotgun sequence.
ACCESSION   DASUTZ010000271 DASUTZ010000000
VERSION     DASUTZ010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 30285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30285
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1661979"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VF097_11615"
     CDS             complement(<1..465)
                     /locus_tag="VF097_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX6263483.1"
                     /translation="MTREALPALRVPDPTLESRVAIRLEEIEAALERAVLSDIRLVAE
                     AGRHLIAAGGKRFRPTLVVLGSHFGDPDVPEITPGAVAIELTHLASLYHDDVIDGADS
                     RRGAPSVNAEWGNTIAVLTGDYLFARSSEISADLGTDVSRLFAATIARVCEGQ"
     gene            complement(490..1929)
                     /locus_tag="VF097_11620"
     CDS             complement(490..1929)
                     /locus_tag="VF097_11620"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX6263484.1"
                     /translation="MAPEIVLRPIVPEMIVAGAGMAILLVDAFRSSRDRLPLALIALA
                     AVVGAAASSVSLWGWDGPATVLGGMVASDRYGVFFRLVILGVAALAILLSLHGRDEER
                     GGEFYALLLFSTTGMTLLAVSADLIVVFLSLEILSLSLYVLTGSSIRRPASQEAGMKY
                     FLLGAFSTAFLLYGIALAYGATGTTSLSGIAGALAGRTDDLGLALAAAALLTVGFSFK
                     VSAVPFHMWTPDVYQGAPTAVTAFMSAGTKVAGFAALLRVASSALGPVGWDLTPALWL
                     LAALTMVVGSVLAIAQRDVKRLLAYSSVAHAGFVLVGVVAANQQGISGAMFYLVAYAA
                     MILGAFGIVMIVAGRDEERTTMEDYAGLARRSPVLAATLGLFLLSLAGLPPTAGFIAK
                     VLVFQAAVDAGHWALVVVGVVATVIAAYFYIRVIVAAYMEEPEDRVAVSRAPVPGFAV
                     GVAAVLTLLFGVLPFLLLGVLDSASILRF"
     gene            complement(1933..3525)
                     /locus_tag="VF097_11625"
     CDS             complement(1933..3525)
                     /locus_tag="VF097_11625"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004500.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX6263485.1"
                     /translation="MSGWPWLSLTLWAPIAGLVILLLLTPLTGRRAREEPEDEGEPPP
                     HRGVLVAGPSARVLLWRWGALAVTLATFLISFGILAFFEGGEPGYQLVERARWVESPN
                     LQYLLGVDGISLFMVLLTTFLMPISVLASWRQHERVPAFMASLLILETALLGTFLALD
                     LLLFFVFFEGMLFPMYLLIGIWGYEQRVYAAVKFFIYTMAGSALLLAAILFLYFRAGA
                     ELGTATFDLAALQGMDLPVVTGRWLFLAFLLAFAVKVPLFPLHTWLPDAHTEAPTAGS
                     VLLAGVLLKIGAYGLIRFNLGLFPEASMYFRELVLVLAAIGVVYGGVVALIQRDVKRL
                     VAYSSVSHLGFVVLGIFAGSVQGMSGGVIQMLNHGLTTGALFLLVGMLYERTHTRDLD
                     RLGGLARQAPVFAGVFLVVALSSLALPGLNGFVGEFLILLGSFFSARPYAVVAAVGLV
                     LSAIYLLWAYQRMMHGGERPELDGFRDLSGREYVVLVPIVALIVAIGVYPKPFLERIE
                     PSAEQPARVVQVVDSPVVAEGG"
     gene            complement(3522..5405)
                     /gene="nuoL"
                     /locus_tag="VF097_11630"
     CDS             complement(3522..5405)
                     /gene="nuoL"
                     /locus_tag="VF097_11630"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX6263486.1"
                     /translation="MMADGPASLIWLALALPLAGAVLLLLLGRPLHRAAGWIASGAVG
                     AAFVVALVAFLDLVARPGDARSLSVRLFDWISVGSFEAPFELRVDPLSLVMVLTVTGV
                     SFLIHVYSIGYMREDPGYPRYFAYLNLFVFSMVTLVLANNFVVLYLGWEAVGLCSYLL
                     IAFWYERKAAADAGKKAFIVNRIGDFGFALGIFLIFQATGSVRFDEVFGAAGGLPEGT
                     ATAISFLLLAGAVGKSAQLPLHVWLPDAMEGPTPVSALIHAATMVTAGVYLVVRAHVL
                     FEASEIALFAVAIVGTVTTIYAATSALGQDDLKRVLAYSTISQLGFMFLAAGVGAYGV
                     AIFHLVTHAFFKAVLFLGAGSVMHATGGVIDVKRLGGLGRVMPWTTLFFGIGGLALVG
                     LPPLSGFFSKDQVLAATYGAGHEWLWLVVLVTAGITALYTTRAFVLTFLGTSRLDEGH
                     PHESPAVMLAPMAVLAAGAAAAGLLGLSATGGLLHGFLGLGEVAHAEGGLSEGALAVI
                     ATAVAVVGVLIGWLIWGSGRIDWAALRVRYGGLQRAMARGWYVDDTYGNLLVLPGKAA
                     SAFLAYVFDKRVVDGAVNGLGRAFRGLAGAGRRVQTGFVRTYALAFLLGVVGLLALVV
                     LRT"
     gene            complement(5425..5724)
                     /gene="nuoK"
                     /locus_tag="VF097_11635"
     CDS             complement(5425..5724)
                     /gene="nuoK"
                     /locus_tag="VF097_11635"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX6263487.1"
                     /translation="MPIAWYLATSGILFSIGTVGVLVRRNALVIFMAIELQLNAVNLT
                     LIAFARQLGEVNGQVLAFFSMVVAAAEVVVGLAIIVSIFRRRASASVDDLSLLKW"
     gene            complement(5727..6299)
                     /locus_tag="VF097_11640"
     CDS             complement(5727..6299)
                     /locus_tag="VF097_11640"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005165.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX6263488.1"
                     /translation="MGELITFWVTAPISVGAAALMLFQRNAVHAALLLVLNQFTLAVF
                     FVLLDAEFLAAVQVIVYAGAIMVLFLFVIMFLGVDRRESLRERLRGQRPLAIVLGLFL
                     VIAILFVVQIGVGLTQRTTEEMPRQASNVEEVGRVLFTDYVFPFEVTSILLIIAAVAA
                     MVLARRSRRVPEAQEPDAAGADATRAEVPR"
     gene            complement(6301..6762)
                     /gene="nuoI"
                     /locus_tag="VF097_11645"
     CDS             complement(6301..6762)
                     /gene="nuoI"
                     /locus_tag="VF097_11645"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEX6263489.1"
                     /translation="MILEILKGLGVTFRSMFRRPVTIDYPKEIKELPPRSHIGRHLLN
                     RYPDGLEKCIGCELCAWACPADAIYVEGEDNDPERPISVGERHARIYQINYLRCIMCG
                     LCVEACPTRALTLTSSFELSWSTREDAILEKEQLLQPPPRLGTGPTTDQGR"
     gene            complement(6759..7898)
                     /gene="nuoH"
                     /locus_tag="VF097_11650"
     CDS             complement(6759..7898)
                     /gene="nuoH"
                     /locus_tag="VF097_11650"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007631737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX6263490.1"
                     /translation="MSGFDAGSWVDWLILLIRVLILFAALMVAVLLTIWAERKLVADM
                     QTRLGPNRAGPFGILITLADGIKLFFKEGLTPASADRPVYLLAPIVSLIPAFIAFAVI
                     PFGTSISLFGREIPFQLADLNIGILYVLALGSLGVYGVVLAGWSSGTKYPLLGAVRSS
                     AQMISYEISLGLGLVAVLMYSGTLQMSEIVARQDTIWNVIPQAPAFFIFGISAMAETN
                     RAPFDLPEAESELVAGFHTEYSGIRFAMFFLAEYLHMITVSAVAVTVFFGGPRGPVFD
                     FLPWLWPLLWFLLKLIALIFVFVWIRATLPRFRYDRLMNFGWKVLIPWGLVWVVATGA
                     IVILPDLYGRDTFLRWAAIAAGAALLASVVAPLFSRKPEQEARTG"
     gene            complement(7895..10270)
                     /locus_tag="VF097_11655"
     CDS             complement(7895..10270)
                     /locus_tag="VF097_11655"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005895.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEX6263491.1"
                     /translation="MSVTLTIDGRTVTVPEGTLIIRAAESIGIEVPRFCDHPYLDPVG
                     ACRTCLVEVEGQAKLVTSCTTPVVPDMVVRTQNTSEGAAKGQRGQLEFLLINHPLDCP
                     VCDRGGECPLQDQTLAFGPAESRYIDPKRVYEKPIELSPQVLLDRERCVLCARCTRFC
                     DEISGERFIELFERGAAEQVSISPGEDFASPFSGNTVQICPVGALTNASYRFAARPFD
                     VKRGDSICPHCACGCGIRVDTRRGEVVRHLARDVPEVNEAWLCDKGRNAFRFPDRDRL
                     ETPMLRVPGLEPASHAEVFAEIAARARGGRAAFLGGARLTDEDAYAMAKLARVAFRTN
                     DLDHRMGVGTGSWDLERLQAAPGTPVTYADVERAAAILVVGLDTREELPILHLRIRKA
                     HRKGGAKVFVVHPRRTALDVAEHVLCPPGGEAAALAELRSGSDEIANALREAGEGVVV
                     LAGPRLAESPEALEQAASLAADVGGRIAYLTRRAGDRGALRAGVHPALLPGGRRLDDP
                     AERAEVEAVWGGSLPAEPGRSTREILEAAAAREIDLLFLVGVDPLTDFPDLQLVRRAL
                     ANAGFTVIVDISLGEYEPYADAVIPAAAFLEKAGHLTDWEGRGQRMAPVRDPIGLSRP
                     EWRMFQELSEVLGADLGFGSLEDVQAELGDLLAPREVPAPAPVPALPRDREGEGLSLF
                     TYPLLVDGGRLSVDADELKAALHREPFLEVHPADADSLGLQDGGRVRIRTERGEAELP
                     VVVTDGIHEGAVFVPYNQEGLRMSELLAGSTITAVELEAVEAPAEALEGAG"
     gene            complement(10267..11595)
                     /gene="nuoF"
                     /locus_tag="VF097_11660"
     CDS             complement(10267..11595)
                     /gene="nuoF"
                     /locus_tag="VF097_11660"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017609850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX6263492.1"
                     /translation="MTTARWEREGSERIDSYLEDGGYETLRTALGMSPDDVVEEVKAS
                     GLRGRGGAGFPTGVKWSFIPKDTGRSVYLACNADEGEPGTFKDRELMERDPHQLLEGI
                     AIASYAVGCHQAFIYMRGEFLEPARRVERAIAEAYDRGLLGKGIAGSDFDLDVVMHRG
                     AGSYECGEETALLESLEGYRGQPRLRPPFPAVAGLYGSPTVINNVETLSTVPHVIRMG
                     AEEFAKLGTEKSTGTKIFCVSGKVERPGNYEAPMGTPARVVIEELAGGVRGGKALKAW
                     VPGGSSTPFLTPDHLDTPMDFESIADQGSLLGTGAVVVLDEDDCIVDAVLRWTQFYAH
                     ESCGKCTPCREGTWWMTKVLHRMEHGGGRLDDIDMMVDVGDNILFKSLCALGDGSVSP
                     IDSSIKYFREEYVEHVKAGGCPRKERPKLEAATPRSGGAAGAIPISEVLP"
     gene            complement(11612..12151)
                     /locus_tag="VF097_11665"
     CDS             complement(11612..12151)
                     /locus_tag="VF097_11665"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX6263493.1"
                     /translation="MIFEGPVREQAEAIVARYPRSRSALLPLLYLAQSVEGHLSREGM
                     REVGGLLGLTTAEVEAVATYYTMYRREPAGRWLLSVCTNVSCALLGARDVHEAARGAA
                     EGDQDVEVEEVECLGACDAAPVVQVNYCNYGRVSEEGVVQLIEALRAGEPPPPARGPR
                     PAPHDETSRILAGLGDDRE"
     gene            complement(12151..13389)
                     /locus_tag="VF097_11670"
     CDS             complement(12151..13389)
                     /locus_tag="VF097_11670"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014351470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX6263494.1"
                     /translation="MAQGVQAQRLAEDLMVINMGPQHPSTHGVLRLVLTLDGETVVDC
                     QPVVGYLHTGIEKNAEYRTWHQGPTFVTRADYLSPFSNELAYALSVERLLGIEAPPRA
                     QAIRVMVCELGRIASHLVWLATTGLELGALSMMLYGFREREKLLDVFEAITGLRMNHA
                     YIRPGGLVMDLPEGAEERIRALLADTDGKVDEYESLLSGNPIWVERNRGVGILTAEQC
                     RRWAVTGPILRAAGVPHDLRTTDPLYEGYDFEIPTREEADCYARYEVRVAEIRESLKI
                     VEQVLDGLPGGRVMVGDPKVAWPAQLEVGPDGIGNSERYVRHIMEESMEALINHFKLV
                     TQGVKVPAGEVYTCVESPRGELGFYVVSDGGVRPYRVRIKDPSFVHVQALPEIVRGGL
                     VADVIASIASVDPVMGGVDR"
     gene            complement(13426..13920)
                     /locus_tag="VF097_11675"
     CDS             complement(13426..13920)
                     /locus_tag="VF097_11675"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX6263495.1"
                     /translation="MSPAELAERLGSRFPDVVVARGEVTVTVGRDELGDALRYLRDEP
                     DLGFDFLSDLSCTDWPGREPRIWMAYHLASGHGMRVRVKAGLPEDDLRIVTATEVHPS
                     ASWHEREVYDFFGVTFDGHPDLRRIELPEEWIGHPLRKDEPLGGVATQYKGAYVPPPD
                     QREL"
     gene            complement(13920..14426)
                     /locus_tag="VF097_11680"
                     /pseudo
     CDS             complement(13920..14426)
                     /locus_tag="VF097_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
     gene            complement(14423..14782)
                     /gene="ndhC"
                     /locus_tag="VF097_11685"
     CDS             complement(14423..14782)
                     /gene="ndhC"
                     /locus_tag="VF097_11685"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX6263496.1"
                     /translation="MLVEYLPIVILAGLAALFAVASLAASALLRPRRPTPAKLAPYEC
                     GIVPEHEPPERFPVKFYVIAMLFIIFDIETIFLFPWAVAFRQLGLFGLVEMVIFIGLL
                     FVAYVYVWKRDGLEWAR"
     gene            complement(14804..16078)
                     /locus_tag="VF097_11690"
     CDS             complement(14804..16078)
                     /locus_tag="VF097_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HEX6263497.1"
                     /translation="MNGFTSSAERVDVLVVGAGPAGSAAAAHLARHGVDVLAVDKAAF
                     PREKVCGDGLTPRGVKQLLELGIDVEERGFVRIDGLRSFGPGVKVELPWPESDAFPSF
                     GLVRTRHDLDELLIRNAEKSGAVVWQETDAERPVLDRWGRVLGAVVRRGEGEPREVRA
                     RFVIAADGASSRFAQQAGVRRDPDRPIGIAARRYFRSPLANERHFLSWLDLEHQGGFL
                     PGYGWIFPVGDGIVNVGAGLLNTARDFRHLSAKKVYEVFLRRLPPELGITEETAQGRL
                     LSGPLPMGMNRAPLAVPGLLVTGDAGGIINPFNGEGIAYAMESGRLAAELIHEALVLD
                     RPGLAQVYPAVLRQRYGKYFSMGRAFVRAIGSPTVMRAAVRYGLPRERLMRFLFKLMA
                     NLTDGRDGDLDDRLIHVLTRLAPSRGGFARVL"
     gene            complement(16119..16577)
                     /locus_tag="VF097_11695"
     CDS             complement(16119..16577)
                     /locus_tag="VF097_11695"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX6263498.1"
                     /translation="MRLLDARRMFGDLDVVHLATTLADGSPHVVPLWFVWREGAIYVS
                     ARRDSVTWRNVERDPRAALTFSRGSEWRDLAGIVIHGRAEPVSAVHPALRGVMSSWYE
                     KYREALSGDGFRAFAEQVERPGMMRVRPLHMAAWDHALWLPGERSGDAAG"
     gene            complement(16625..17707)
                     /locus_tag="VF097_11700"
     CDS             complement(16625..17707)
                     /locus_tag="VF097_11700"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX6263499.1"
                     /translation="MRVADVPLPAACLELAAAVEEDLARFLQGERAEAEERAPGVGEL
                     FDELERVIAAGGKRLRPVFCLLGHRAARGEVGPEVIRAAASLELLHTFAIVHDDVMDG
                     SRTRRGAPATWAHLADLHRAERMRGSGEVFGVSGAILAGDLALILADGAFLGAGLAPG
                     ALLPALARYNRMRVEVVAGQYLDVAAAHRGEAAEPEARRIAGLKSGNYTVEGPLQIGA
                     ILAGASGELLTALSAYGMPLGEAFQLRDDVLGVFGDPALTGKPRDSDLREGKRTVLIA
                     RTLERAERGEAAFLLERLGAPDLTEADVERIRGIIESSGALDAVGGLVAELAGRARAA
                     LDVAEVPGDVRAALADLVETCTLRIS"
     gene            complement(17716..18675)
                     /locus_tag="VF097_11705"
     CDS             complement(17716..18675)
                     /locus_tag="VF097_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263500.1"
                     /translation="MTRTTTLALALALGLVSAACGSEGDEGPQAGPSPSPGGGELVAQ
                     VASYDLATGTASRFIVGLLTQDNRFVSGGEATFRFSFIGGGQPEPGPEVTAEFLAIPG
                     EDPEHEEAHAGPPAEGRGVYAAREVTFDRAGIWQVEVTADVDGEPLSGTAAFQVAGEH
                     AYPVVGDRAPRTDNLTLDDIGEAPPGAIDSRAEGGEEVPDPELHDSTIAEAIRRGRPV
                     VAVFATPVYCVSQFCGPITDMIAELESEYRDRAEFVHVEVWRDFQNNVVNRAAADWIL
                     RNDNIFEPWVFFVDAEGVIRGRWDNVATREEVEAFLRDLPPMG"
     gene            18738..20519
                     /locus_tag="VF097_11710"
     CDS             18738..20519
                     /locus_tag="VF097_11710"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX6263501.1"
                     /translation="MKLVRRLSVVTVIATLGLVALGGVVRATGSGLGCPDWPRCHGRL
                     IPPLEFHALIEYSHRLLASIVVILVIATAWAAWRRARGTPQVLRPAVLAIGVVLLQAG
                     LGAVVVAEELAPLPNTLHFATAMLLVATVVTTAANATLGRAARGEAPRATPGMRRLLW
                     WALGVTAAALLTGVYMRGSGSSLAFLDWPLMDGRAIPELRTESAWAAFLHRAIAALAV
                     VLILGVAVRARELAPRIRWLARTATGLVLLQSALGAATVFTRLDALPVAGHILGSSLT
                     WASLVALVAGTRPLRHPEPVEEADRPGARQVAGAYLQLMKPDIIVLLLVTTIPTMILA
                     AGGLPPVGLVAATLLGGTLAAGGANALNHFLDRDIDEVMDRTRARPIPAHRVEPDDAK
                     SFGILLSGLSFAWLALTVNLLSAALALSAILFYVLVYTAWMKRSTPQNIVIGGAAGAV
                     PALVGWAAVTGTLALPAWVLFAVIFFWTPPHFWALSMKYADDYQAAGVPMLVVLHGSA
                     DTATHILLYSIQTVAVSLLLFPAAGMGTLYLAAAVGLGGILIWYAARVVRERTTRAAM
                     HLFHYSITYLGLLFAAVAADTLIGALA"
     gene            20516..20731
                     /locus_tag="VF097_11715"
     CDS             20516..20731
                     /locus_tag="VF097_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263502.1"
                     /translation="MIELFGDVIRNHGWHVVAELVLLALATVFTAFYVAALVRGRVRA
                     VECAGCGRIASRAHTECPRCRTPLPTS"
     gene            20805..21917
                     /locus_tag="VF097_11720"
     CDS             20805..21917
                     /locus_tag="VF097_11720"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX6263503.1"
                     /translation="MRSDRARRTPSTRRRWWLTGFVVVTGLLLAACQQGLPQSSLEPA
                     GPRAERIDDLSTIVFWMAGVVFVLVEGAIIFITIRYRRRRREDQAPVQIHGNNRLEII
                     WTVIPALLLAGLAFPTVAAIFDLSRRPPGGDVLEVNVIGHQWWWEFRYSDLNIVTANE
                     LVIPVDTPVHLSLESAETPGGIAEFTADGQPVTQNAIPVIHSFWVPRLAGKQDLVPGK
                     VETLTIEASETGTFLGQCAEYCGTSHANMRKRVIVETSDGFQEWVSRMQAPAAEPEPG
                     SLAAQGAQFFQNFGTGSCLACHGVEPGAGGTVGPNLAHLASRRTFAAGLFETNRENLQ
                     RWLDNPRAMKPGVVMPDYNLTEEQIEAITEYLLTLE"
     gene            21937..23817
                     /gene="ctaD"
                     /locus_tag="VF097_11725"
     CDS             21937..23817
                     /gene="ctaD"
                     /locus_tag="VF097_11725"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEX6263504.1"
                     /translation="MAIAQPLPRGSVFRRPTATTGFVSWLTTVDHKRIGILYGVTSFA
                     FFLLGGIEALLIRWQLATPEGQLLDADVYNQLFTMHGITMIFLVVMPLSSAFFNYLVP
                     LMIGARDVAFPRLNAFSYWIFLLGGLFLYSSWFLGGAPDGGWFAYAPLSSQMPGNNLD
                     FYAVGLQIVGVASLVSAVNFIVTIFNERAPGMSLMRMPVFVWMTLVVSFLLLFALPVI
                     AIALFQLAGDRILGTHFFDAAAGGDPILWQHMFWIFGHPEVYILILPAMGIVSEILPT
                     FSRKPLFGYPAVVFAGAAIGFMGWGVWAHHMFTSGLGPVANSAFALSTMFIAVPTGIK
                     IFNWLGTMWGGAIRFTTPMLMAIGFVAMFVIGGLSGVTHSIVPADWQQHDTYYIVAHF
                     HYVLFGGAIFGLFGGAYYWFPKFTGRTYSERLGHLHFWLMLIGFNLTFAPMHLLGLEG
                     MPRRIYTYGEGLGFDVWNMVSTIGAFTIAASILVFMVNVVRSLSRGEKAPPDPWDGRT
                     LEWTIPSPPPHYNFAEVPQVRARDDFWHRKYTEDGSGRVQPVVAGAATEEAEEEEHAE
                     EEHLDIHMPDPSYWPLVAAAGLPFLGYGLIYDWIWAVPGAALLLFGLFGWSLEPVAEE
                     GH"
     gene            23817..24410
                     /locus_tag="VF097_11730"
     CDS             23817..24410
                     /locus_tag="VF097_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX6263505.1"
                     /translation="MAQAQVRHEAHTATGLDNRKLGMWAFLGSECLFFGALITTYLLY
                     RGRSVQGPFPEDIYDIPFTSVSSFVLLMSSLTMVLALSAIQRGDHQGLRIWLLATAFL
                     GMTFLGGQVYEFTVFVNEGLTVTRNVFGSAFFVLTGFHGLHVTVGVLMLLHHFGMSVA
                     GRIGQEDSLRIELVGLYWHFVDIVWVIIFTVVYLIPR"
     gene            24407..24757
                     /locus_tag="VF097_11735"
     CDS             24407..24757
                     /locus_tag="VF097_11735"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEX6263506.1"
                     /translation="MTEDAHVAGRTEEDPRTHARALEHAHPAPAEYVKVAVALAVVTA
                     LEVGLFYIEGLADGLLIALLMVLMAIKFAMVAMWFMHLKFDNKIFSRLFITGILLAIG
                     VFTVVLVTIGVVGD"
     gene            24757..25596
                     /locus_tag="VF097_11740"
     CDS             24757..25596
                     /locus_tag="VF097_11740"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX6263507.1"
                     /translation="MPAWHPHPDAWLLVAVIVATYVYGLRRVGPRHVSPGDPPASRGQ
                     LIAFGLGVLALLISADWPIHDLGERSLFTAHMLQHLLLTLVAPPLLLLGTPGWMLRWL
                     LRPRWLLSLVRALSKPLVALVLFNLVIAVIHWPAFVSLYLREPLVHLATHAALLGTAL
                     LMWMPVLSPIVEIARLSYPLQMLYLFAQSIVPTVPASFLTFGSEPLYRIYEELPRLWG
                     ISALTDQRVAGLTMKLLGGLILWGVITILFFRWAKQEEEGVDDLGWRDVEEAVNPKEL
                     LTR"
     gene            25593..26273
                     /locus_tag="VF097_11745"
     CDS             25593..26273
                     /locus_tag="VF097_11745"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX6263508.1"
                     /translation="MSEDRRRRSLLAIFVPLTAVLAIAIVLWSMSRILLAASVNVAPV
                     IALLFALNILIAATLAATFRRRTGVVLAFVMAVLVPVLAVGIVGAVAGEREFHSAIEE
                     EPEPGPSPTAEPTEEPTEEPTEEPTGPSEGPTEATIVALNIQFDTDAMQLAANAEVTV
                     TLDNQDAGVPHNWAMYTDESASQEIFVGEIITGPETIDYSFTSPDPGEYYFHCDVHPN
                     MRGTVTVG"
     gene            complement(26274..27257)
                     /gene="ccsB"
                     /locus_tag="VF097_11750"
     CDS             complement(26274..27257)
                     /gene="ccsB"
                     /locus_tag="VF097_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014378140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEX6263509.1"
                     /translation="MTEASNTTFGVALALYLVAMVGYLHHFAYRRPTPLTVARVVAYL
                     GLASHTVSVVLRGVAAERVPWGNMYEYASVVGLAVVALYLVVAERRVDARRLGGFVLG
                     ATVLIMAASTMVYVEPGPLIPALNSGWLRIHVLAAILASALFTLGFVFTVLYLIQERR
                     ERRVERAVAERPAPAMASVGSGLPHDLEVEADPEEGRATVTLGPEQGNGHGGMREGPT
                     RRGRLPEAAALDDLAAKAITFAFPIWTFAVIAGAIWAEQAWGRYWGWDPKEVWSFVTW
                     VLFAGYLHARATQGWRGRRAAVLAVLGFAALAFNLTVVNLVVSGLHSYAGV"
     gene            complement(27254..28732)
                     /locus_tag="VF097_11755"
     CDS             complement(27254..28732)
                     /locus_tag="VF097_11755"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEX6263510.1"
                     /translation="MATGTFSRGMLSVWRSLRSMRTALILLLLLGLGSVVGSLVPQAP
                     NSPARVAELFADRPLLARIYDALGFFDVFGSWWFTLLYVLLLISLAACLIPRTRGLIR
                     GLGSRWQPARDLEALRHYASGSVDGSPEEAVGRARRALRRRGWGVRSGGETALAAEKG
                     LAREVGSLAFHWAFFLLLIGVVYGKGFGFTGQATVIEGDVFTEAHASYDFPPREGRFF
                     GERHQGFQVRVLDFEVTYREGGVPRDFVSRVDVVEEGEVVRTDEIRVNDPLEHRGVKL
                     YQSGYGWAPVVEVRHEGELLVSDPLVFVTDDPNDPVRPWRGAIKLPALSPQVGLELQL
                     LPDAAAFVTGMPMLEADDPFIAYRAYRGDLRLTEAQSVFRLEKELLEEYDSGGLALGE
                     TVELPGGLEVTFRDLRRYTQLLVKRDPGIGLMLATALLVLVGLVPALYSSRRRIWVRA
                     VPEDGGTRLEIGGFALQRKAAFDEEFDRLVRDLVPRGRVGVP"
     gene            complement(28758..29531)
                     /locus_tag="VF097_11760"
     CDS             complement(28758..29531)
                     /locus_tag="VF097_11760"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX6263511.1"
                     /translation="MDLNVWWAPALAFAAGVVSFASPCVLPLVPGYVGFVLGQGPDGR
                     PASPSGDGAVAVAERPATRAALPMLLFVAGFALVFTLLGAFSRVFAEIFRHPWGQRIA
                     GGIVVLFGVYLILYSLRIGGPRLFAERRPFLSKVRPGTAGAFPLGMAFATGFTPCIGP
                     VLAGILTIAAAQGGGAWGALLMFTYSLGLGLPFVLVGLGLRRLMGALAWVKRNYHWIA
                     GIGGVLMVVIGVLLMLNLWTRFLAWSGLLDLVSGFEPPI"
     gene            29701..30063
                     /locus_tag="VF097_11765"
     CDS             29701..30063
                     /locus_tag="VF097_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263512.1"
                     /translation="MRLPIPSSVRRRMEPYKERADDFLRRWEWTWTSAFIAGLIISFI
                     AMTTLVVIPSWFLFFANQTLGWSTADRLLVVIRDSITMGWITVWTVAFIFVAYKVQVH
                     RRRLRGERQAERYSGGYR"
     gene            30082..>30285
                     /locus_tag="VF097_11770"
     CDS             30082..>30285
                     /locus_tag="VF097_11770"
                     /inference="COORDINATES: protein motif:HMM:NF024207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX6263513.1"
                     /translation="MAFQKTEVNIHEDYTITPVDPEWLKEGVKSPKHLMLDPDPCILC
                     RACEDVCPWNCIYMLDPGIVENPG"
BASE COUNT         4719 a        11103 c        10343 g         4120 t
ORIGIN      
        1 ctgcccctca cacaccctcg cgatcgtggc ggcgaacagc cgggagacgt cggtccccag
       61 gtccgccgag atctcggatg acctggcgaa caggtagtcc ccggtgagga cggcgatcgt
      121 gttcccccac tccgcgttca ccgagggcgc gccgcgccgc gagtcggcgc cgtcgatcac
      181 gtcgtcgtgg tagagcgagg ccaggtgggt gagctcgatc gccacggcgc cgggcgtgat
      241 ctcggggacg tcggggtcgc cgaagtggga tccgagcacg acgagcgtgg gccggaaccg
      301 cttccccccc gcggcgatca ggtggcgtcc ggcctcggcc acgaggcgga tgtcggagag
      361 cacggcccgc tccagcgcgg cctcgatctc ctcgagccgg atcgccacgc gcgactccag
      421 cgtggggtcg ggcacccgca gcgccggaag agcctcacgc gtcatgggag gcgatggtat
      481 cagcgggcat cagaacctca ggatcgacgc gctgtccaga acgccgagga ggaggaaggg
      541 caggaccccg aacaggagcg tgagcaccgc cgccacgccc accgcgaacc ccgggaccgg
      601 agcccgggag accgcgacgc gatcctcggg ctcctccatg tacgcggcca cgatcacgcg
      661 gatgtagaag tacgcggcga tcaccgtggc gactaccccg accaccacga gcgcccagtg
      721 gccggcgtcc acggccgcct ggaacacgag gaccttggcg atgaacccgg ccgtgggagg
      781 cagcccggcc agggagagca ggaacagacc gagcgtcgcc gcgagcaccg ggctgcgccg
      841 ggccaggccc gcgtagtcct ccatcgtggt ccgctcctcg tcgcggccgg ccacgatcat
      901 cacgatcccg aacgccccga ggatcatggc cgcgtaggcc accaggtaga acatcgcccc
      961 cgagatcccc tgctggttgg cggccaccac gcccacgagc acgaatccgg cgtgggccac
     1021 cgaggaatac gccaggagcc gcttcacgtc ccgctgcgcg atggccagca ccgagcccac
     1081 aaccatggtc agcgccgcca gcagccagag ggccggggtg aggtcccagc ccaccgggcc
     1141 cagcgccgac gaggccacgc ggagcagcgc cgcgaacccg gccaccttcg tcccggccga
     1201 catgaaggcc gtcaccgcgg tgggcgcccc ctggtacacg tcgggggtcc acatgtggaa
     1261 cgggaccgcc gacactttga acgagaaccc caccgtcagg agcgcggcgg cggccagcgc
     1321 cagcccgagg tcgtcggtcc tcccggccag ggccccggcg atccccgaga ggctggtggt
     1381 gccggtggcc ccgtaggcca gggcgatgcc gtagaggagg aacgcggtag aaaaggctcc
     1441 caggaggaag tacttcatcc ccgcctcctg cgacgcgggg cgccggatcg aggagccggt
     1501 cagcacgtac agcgagagcg agaggatctc cagggagagg aagaccacga tgagatcggc
     1561 cgagacggcc agcagcgtca tccccgtggt ggagaagagc agcaacgcgt agaactcgcc
     1621 gccccgctcc tcgtccctcc cgtggaggga gaggaggatc gccagcgcgg cgacgcccag
     1681 gatcacgagc cggaagaaga cgccgtagcg atcggaggcg accatcccgc ccagcaccgt
     1741 cgccggcccg tcccatcccc agagcgagac agacgaggcc gccgccccga cgacggcggc
     1801 cagggcgatc agcgccagcg gcaggcggtc ccgcgaggag cggaacgcgt cgaccagcag
     1861 gatcgccatc ccggcccccg ccacgatcat ctcggggacg atcggtcgca gcacgatctc
     1921 cggcgccacg gctcagcctc cctccgccac gaccggggag tccacgacct ggaccacgcg
     1981 cgccggctgc tcggccgagg gctcgatccg ctcgaggaac ggcttcgggt agaccccgat
     2041 cgccacgatc agggccacga tcgggaccag gacgacgtac tcgcggcccg agaggtcgcg
     2101 gaacccgtcg agctccggcc gctccccacc gtgcatcatc cgctggtagg cccagaggag
     2161 gtagatcgcc gagaggacca gcccgacggc cgccaccaca gcgtagggcc gggccgagaa
     2221 gaacgacccc agcaggatga ggaactcgcc cacgaacccg ttcaggcccg ggagggccag
     2281 cgacgagagc gccaccacga ggaacacgcc ggcgaacact ggggcctgcc tggccaggcc
     2341 tccgagccga tcgaggtccc tcgtgtgggt ccgctcgtac agcatcccca cgaggaggaa
     2401 cagcgcgccc gtcgtcagcc cgtggttcag catctggatc accccgcccg acatcccctg
     2461 gacgctcccg gcgaagatac ccaggaccac gaaccccagg tgggagacgc tcgagtacgc
     2521 cacgaggcgc ttcacgtcgc gctggatcag cgccaccacg cccccgtaaa ccaccccgat
     2581 cgcggcgagc acgagcacga gctcgcggaa gtacatcgac gcctcgggga agagcccgag
     2641 gttgaaccgg atcaggccgt aggccccgat cttcagcagg accccggcca gcagcaccga
     2701 ccccgccgtg ggcgcctccg tgtgcgcgtc gggcagccac gtgtggagag ggaacagcgg
     2761 caccttcacc gcgaacgcca gcaggaacgc caggaacagc caccggccgg tgaccacggg
     2821 gaggtccatc ccctgcagtg ccgccaggtc gaaggtcgcc gttcccagct cggccccagc
     2881 ccggaagtag aggaagagga tcgccgcgag cagcagggcc gagcccgcca tcgtgtagat
     2941 gaagaacttc acggccgcgt agacccgctg ctcgtacccc cagatcccga tcaggaggta
     3001 catcgggaag agcatcccct cgaagaacac gaagaagagg aggaggtcca gggccaggaa
     3061 cgtgccgagc agcgcggtct ccaggatcag cagcgaggcc atgaacgccg ggacccgctc
     3121 gtgctggcgc cacgacgcca gcaccgagat cggcatcagg aacgtggtga gcaaaaccat
     3181 gaagaggctg atcccgtcca ccccgaggag gtactggagg ttcggggact ccacccaccg
     3241 agcccgctcc acgagctggt acccgggctc ccctccctcg aagaacgcga ggatcccgaa
     3301 cgagatcagg aaggtggcca gcgtgacggc gagcgccccc caccgccaga ggagaacccg
     3361 ggccgacggc cccgccacga ggaccccccg atggggggga ggctcgccct cgtcctccgg
     3421 ctcctcccgg gcccgccgcc cggtgagcgg cgtcagcagc agcaggatca cgagccccgc
     3481 gatcggggcc caaagcgtga gcgagagcca gggccagccg ctcatgtcct gagcaccacg
     3541 agggccagga gccccacgac ccccagcagg aacgccagcg cgtaggtgcg gacgaagccg
     3601 gtctggaccc gccgacccgc ccccgccagg cctcggaaag ccctccccag gccgttgacg
     3661 gcgccgtcca ccacgcgctt gtcgaagacg taggcgagga acgccgaggc cgccttcccg
     3721 gggagcacca ggaggttccc gtatgtgtcg tccacgtacc agccgcgggc catggcccgc
     3781 tggaggccgc cgtagcgcac ccgcagggcg gcccagtcga tccgaccgga cccccagatc
     3841 agccagccga tcaggacgcc gaccacggcc acggccgtgg cgatcaccgc cagggctccc
     3901 tccgagagtc ctccctcggc gtgggccacc tcccccagcc ccaggaatcc atggaggagg
     3961 ccgccggtgg ccgagaggcc gagtaggccc gcggcggcgg ccccggccgc cagcaccgcc
     4021 atcggggcga gcatcaccgc cggggattcg tgaggatgcc cctcgtcgag gcgcgacgtc
     4081 cccaggaacg tcagtacgaa cgcccgggtc gtgtacagcg cggtgatccc cgccgtgacc
     4141 agcaccacga gccacagcca ctcgtggccg gccccgtacg tcgccgccag cacctggtcc
     4201 ttggagaaga agccggacag cgggggcagc cccacgagcg ccagcccccc gatcccgaag
     4261 aagagcgtcg tccacggcat gacccgcccc aggccgccga gccgcttaac gtcgatcacg
     4321 ccgccggtgg cgtgcatcac gctgcccgcg ccgaggaaca gcacggcctt gaagaacgcg
     4381 tgcgtcacga ggtggaagat ggccacgccg tacgccccca cgcccgccgc caggaacatg
     4441 aacccgagct gggagatcgt ggagtaggcg aggacccgct tgaggtcgtc ctggcccagg
     4501 gccgaggtcg cggcgtagat cgtcgtgacg gtccccacga tcgccaccgc gaacagcgcg
     4561 atctccgagg cctcgaacag cacgtgggcc cgcacgacga ggtacacgcc ggccgtcacc
     4621 atcgtcgcgg cgtggatcag ggccgagacg ggggtcgggc cctccatcgc gtcgggcagc
     4681 cagacgtgga gcgggagctg ggccgacttc ccaacggccc ccgccaggag caggaacgag
     4741 atcgcggtag ccgtcccctc gggcagaccg cccgccgcgc cgaagacctc gtcgaaccgc
     4801 accgaccccg tggcctggaa gatcaggaag atccccagtg cgaacccgaa gtccccgatc
     4861 cggttgacga tgaacgcctt cttccccgcg tcggccgcgg ccttccgctc gtaccagaac
     4921 gcgatcagca ggtaggagca gagtcccacc gcctcccacc ccaggtacag cacgacgaag
     4981 ttgttcgcca ggacgagcgt gaccatcgag aagacgaaga ggttgaggta ggcgaagtag
     5041 cgcgggtacc ccgggtcctc gcgcatgtag ccgatcgagt agacgtggat caggaacgag
     5101 acgccggtca ccgtcagcac catcaccagc gagagcggat ccacccggag ctcgaacggc
     5161 gcctcgaacg accccaccga gatccagtcg aacagccgca ccgagaggct gcgggcgtct
     5221 ccgggtcggg cgacgaggtc caggaacgct accagggcca cgacgaaggc ggcgcccacg
     5281 gccccggagg cgatccaccc ggcggcgcga tgcagcggcc tccccagcag aaggagcagc
     5341 accgcccccg ccaagggcag ggcgagcgcc agccagatca agctcgcggg cccgtccgcc
     5401 atcatcgctc cctccatcgc cggatcacca cttcaggagg ctgaggtcgt ccacgctggc
     5461 cgaggcccga cgccggaaga tcgagacgat gatggccagg ccgaccacga cctcggccgc
     5521 cgccacgacc atcgagaaga aggccagcac ctggccgttc acctcgccca gctggcgggc
     5581 gaacgcgatc agcgtcaggt tcaccgcgtt cagctggagc tcgatggcca tgaagatcac
     5641 gagcgcgttc cgccgcacga gcacgccgac ggtgccgatc gagaagagga tcccggacgt
     5701 ggccaggtac caggcgatcg ggatcctcac ctcggcacct ccgccctcgt cgcgtccgcc
     5761 ccggcagcgt ccggctcctg cgcctccggg acgcgccggc tccgccgcgc caggaccatc
     5821 gccgccaccg ccgcgatgat cagcaggatc gacgtcacct cgaacgggaa gacgtagtcg
     5881 gtaaagagga cgcggcccac ctcctccacg ttgctcgcct gccggggcat ctcctcggtc
     5941 gtccgctggg tcagcccgac cccgatctgc acgacgaaca ggatcgcgat cacgaggaac
     6001 aggccgagga cgatcgccag gggccgctgg ccgcgcaggc gctcgcggag gctctcccgg
     6061 cggtccacgc cgaggaacat gatcacgaac aggaagagga ccatgatcgc gcccgcgtag
     6121 acgatcacct ggaccgccgc caggaactcc gcgtccagca ggacgaagaa caccgccagc
     6181 gtgaactggt tgaggaccag gagcagcgcc gcgtggaccg cgttccgctg gaagagcatc
     6241 agggccgcgg cccccaccga gatcggggcc gtcacccaga acgtgatcag ctcgcccatc
     6301 tcaccgcccc tgatcggtcg tgggccccgt cccgaggcgc ggcggcggct ggagcagctg
     6361 ctccttctcc aggatcgcgt cctcccgcgt ggaccaggac agctcgaagc tgctcgtcag
     6421 cgtgagcgca cgcgtggggc aggcctcgac gcacaacccg cacatgatgc agcggaggta
     6481 gttgatctga tagatgcggg cgtgccgctc tcccaccgag atcggccgct cgggatcgtt
     6541 gtcctcgccc tcgacgtaga tcgcgtcggc gggacaggcc caggcgcaga gctcgcagcc
     6601 gatgcacttc tcgagcccgt cggggtaccg gttgagaagg tgccggccga tgtgcgaccg
     6661 cggcggcagc tccttgatct ccttggggta gtcgatcgtg acgggccggc ggaacatgct
     6721 ccggaaggtc acgccgaggc ccttgaggat ctccaggatc atccggtcct cgcctcctgc
     6781 tcaggcttcc gcgagaagag gggcgccacg accgaggcga gcagggccgc gcccgcggcg
     6841 atcgccgccc accgcaggaa ggtgtcacgc ccgtagaggt ccgggaggat cacgatcgcc
     6901 cccgtcgcca ccacccagac cagcccccag gggatcagca ccttccatcc gaagttcatc
     6961 aggcggtcgt agcggaaccg cggaagcgtg gctcggatcc agacgaacac gaagatgagc
     7021 gcgatcagct tcaggaggaa ccaaagcaac ggccagagcc acgggaggaa gtcgaagacc
     7081 ggcccgcgcg gacccccgaa gaagacggtg accgccacgg ccgacaccgt gatcatgtgg
     7141 aggtactcgg ccaggaagaa catcgcgaag cggatcccgg agtattcggt gtggaagccg
     7201 gccacgagct cggactcggc ctcggggagg tcgaacggag cgcggttcgt ctccgccatc
     7261 gcggagatcc cgaagatgaa gaacgccggc gcctggggga tcacgttcca gatcgtgtcc
     7321 tggcgagcca cgatctcgga catctgcagc gtgcccgagt acatcagcac ggccacgagc
     7381 ccgaggccga gcgagatctc gtaggagatc atctgggcgc tcgaccgcac cgctccgagc
     7441 agggggtact tggtcccgga ggaccagccg gccagcacga ccccgtagac cccgagcgat
     7501 ccgagcgcca ggacgtagag gatgccgatg ttgaggtcgg cgagctggaa cgggatctcc
     7561 cgcccgaaca ggctgatcga ggtcccgaac gggatcacgg cgaaggcgat gaacgcgggg
     7621 atcagcgaga cgatcggcgc caggaggtag acggggcggt cggccgaagc cggcgtcagg
     7681 ccctccttga agaagagctt gatcccgtcg gcgagcgtga tgaggatccc gaagggcccc
     7741 gcgcggttcg ggcccagccg cgtctgcatg tccgccacga gcttccgctc ggcccagatc
     7801 gtgagcagaa cggcgaccat cagcgcggcg aacaggatca ggacgcggat caggaggatc
     7861 agccagtcca cccagctgcc cgcgtcgaag ccgctcatcc cgctccctcc agggcctcgg
     7921 ccggcgcctc gaccgcctcc agctccacgg ccgtgatcgt ggaccccgcg agcagctcgc
     7981 tcatccgcag gccctcctgg ttgtagggga cgaagaccgc gccctcgtgg atgccgtcgg
     8041 tgacgacgac ggggagctcg gcctcgcccc gctcggtccg gatccgaacc cggccgccgt
     8101 cctgcaggcc caggctgtcc gcgtcggccg ggtggacctc gaggaagggc tcgcggtgga
     8161 gggccgcctt cagctcgtcg gcgtccacgg agaggcggcc gccgtccacc agcagggggt
     8221 aggtgaagag cgagaggccc tccccctcgc ggtccctcgg gagcgccggc acgggcgccg
     8281 gcgccgggac ctcgcggggg gcgaggaggt cgcccagctc ggcctgaacg tcctccaggg
     8341 agccgaagcc caggtccgcc cccaggacct cggagagctc ctggaacatc cgccactcgg
     8401 gccgcgagag gccgatcggg tcgcggaccg gcgccatgcg ctggccgcgc ccctcccagt
     8461 cggtgagatg gccggccttc tccaggaagg ccgcggcggg gatgaccgcg tcggcgtacg
     8521 gctcgtactc gcccagcgag atgtccacga tcaccgtgaa gccggcgttg gccagggctc
     8581 ggcggacgag ctgcaggtcg gggaagtcgg tcagtggatc cacgccgacg aggaagagga
     8641 ggtcgatctc ccgggcggcc gccgcctcga ggatctcgcg ggtgctgcgg ccgggctccg
     8701 ccgggagcga gccgccccag accgcctcca cctcggcccg ctccgcgggg tcgtcgaggc
     8761 ggcggccgcc cgggagcagg gcggggtgga cgcccgcgcg gagcgcgccg cggtctcccg
     8821 cgcgccgggt gaggtaggcg atcctcccgc ccacgtcggc agcgagggag gcggcctgct
     8881 ccagggcctc cgggctctct gcgagccgcg ggccggccag taccacgacc ccctcccccg
     8941 cctcgcggag ggcgttggcg atctcgtccg acccggagcg gagctccgcg agcgcggcgg
     9001 cctcgccgcc cggcgggcac agcacgtgct cggcgacgtc gagcgcggtg cggcgagggt
     9061 ggacgacgaa gaccttggcc ccacccttgc gatgcgcctt ccggatgcgc agatgcagga
     9121 tgggcagctc ctccctggtg tcgagcccca cgacgaggat cgccgcagcg cgctcgacgt
     9181 cggcgtacgt caccggcgtg ccgggggcgg cctggaggcg ctccaggtcc cacgaccccg
     9241 tgcccacacc catccggtgg tcgaggtcgt tcgtgcggaa ggccacgcgg gccagcttgg
     9301 ccatggcgta ggcgtcctcg tcggtgaggc gcgcgccgcc caggaaggcg gcgcgccctc
     9361 ctctcgcccg cgccgcgatc tccgcgaaca cctcggcgtg cgatgccggc tcgaggcccg
     9421 ggacacgcag catcggcgtc tccagccggt cccggtcggg gaaccggaac gcgttccggc
     9481 ccttgtcgca cagccaggcc tcgttgacct ccggcacgtc ccgcgccagg tgccgcacga
     9541 cctcgccccg ccgggtgtcc acccggatgc cgcacccaca cgcgcagtgc gggcagatcg
     9601 aatcgccccg cttcacgtcg aagggccgtg cggcgaaacg gtaggaggcg ttcgtcagcg
     9661 cccccaccgg gcagatctgg acggtgttgc cgctgaaggg cgaggcgaag tcctcgcccg
     9721 gcgagatcga gacctgctcg gcggcccccc gctcgaacag ctcgatgaac cgctcccccg
     9781 agatctcgtc gcagaaccgc gtgcaccggg cgcacaacac gcaccgttcg cggtccagca
     9841 ggacctgtgg ggagagctcg atcggcttct cgtagacccg cttgggatcg atgtagcgac
     9901 tctcggccgg cccgaacgcc agcgtctggt cctgcagggg gcactcgccg ccgcggtcgc
     9961 acaccgggca gtcgagcggg tggttgatca ggaggaactc gagctggccc cgctggccct
    10021 tggccgcgcc ctcgctcgtg ttctgggtgc gcacgaccat gtccgggacc accggtgtcg
    10081 tgcacgacgt cacgagcttg gcctgtccct cgacctcgac gagacacgtc cggcacgccc
    10141 ccaccgggtc gaggtacggg tggtcgcaga agcgcggcac ctcgatcccg atcgactccg
    10201 ccgcccggat gatcagcgtc ccctcgggta ccgtgacggt ccgcccgtcg atcgtcagcg
    10261 tgacgctcac ggcaggacct ccgagatcgg gatcgcgccc gccgccccgc ccgaccgcgg
    10321 ggtcgcggcc tcgagcttcg gccgctcctt cctgggacag cctcccgcct tcacgtgctc
    10381 cacgtactcc tcccggaagt acttgatcga gctgtcgatc ggcgagaccg agccgtcccc
    10441 cagcgcgcag agcgacttga agaggatgtt gtcccccacg tcgaccatca tgtcgatgtc
    10501 gtcgagccgc ccgccgccgt gctccatccg gtggaggacc ttcgtcatcc accaggtccc
    10561 ctcccggcac ggcgtgcact tcccgcacga ctcgtgggcg tagaactgcg tccaccgcag
    10621 cactgcgtcc acgatgcagt cgtcctcgtc gaggaccacc accgcgccgg tccccagcag
    10681 ggacccctga tcggcgatcg actcgaagtc catcggggtg tcgaggtggt cgggcgtgag
    10741 gaagggcgtg gacgagcccc ccggcaccca ggccttgagg gccttccccc cacggacccc
    10801 gcccgcgagc tcctcgatga cgacccgggc cggcgtgccc atcggggcct cgtagttgcc
    10861 gggccgctcc accttcccgg agacgcagaa gatcttcgtc ccggtggact tctcggtgcc
    10921 gagcttcgcg aactcctcgg ctcccatccg gatcacgtgc gggaccgtgg agagcgtctc
    10981 gacgttgttg atcacggtgg gcgagccgta gagaccggcc accgcgggga agggcggccg
    11041 cagacggggc tggcctcggt agccctcgag cgactcgagg agcgccgtct cctcgccaca
    11101 ctcgtacgag cccgcgccgc ggtgcatcac gacgtcgagg tcgaagtcgc tccccgcgat
    11161 ccccttcccc aggaggccgc ggtcgtaggc ctcggcgatc gcacgctcga cgcggcgggc
    11221 cggctccagg aactcgcccc gcatgtagat gaacgcctgg tggcacccca ccgcgtagga
    11281 ggcgatcgcg atcccctcca gcagctggtg cgggtctcgc tccatcagct cccgatcctt
    11341 gaaggtgccg ggctccccct catctgcgtt gcaggcgagg tagacggacc tgcccgtgtc
    11401 cttcgggatg aagctccact tcacgccggt ggggaagccg gcgccgccgc ggccccggag
    11461 ccccgaggcc ttcacctcct ccaccacgtc gtccggcgac atcccgaggg ccgtccgcag
    11521 cgtctcgtag ccgccgtcct cgaggtagga gtcgatcctc tccgagccct ccctctccca
    11581 ccgggcggtg gtcagcgtgg ggaagcgctc gtcactcacg gtcgtctccc aggccggcca
    11641 ggatccggga ggtctcgtcg tgcggcgcag gcctcgggcc acgggccggg ggcggcggct
    11701 cgccggcccg cagggcttcg atcagctgga cgaccccctc ctcgctcaca cggccgtagt
    11761 tgcagtagtt gacctggacc accggggccg cgtcgcaggc cccgagacac tccacctcct
    11821 cgacctcgac gtcctggtcg ccctcggccg ccccgcgggc ggcctcgtgc acgtcccggg
    11881 ccccgagcag agcgcacgag acgttcgtgc agaccgagag gagccatcgg ccggccggtt
    11941 ctcgccgata catcgtgtag taggtggcga cggcctccac ctcggccgtg gtgagcccta
    12001 ggagcccgcc cacctcccgc atgccctcgc gcgagaggtg cccctccacg gactgcgcca
    12061 ggtagaggag cggcaggagg gccgaccgcg accgtgggta ccgggccacg atcgcctcgg
    12121 cctgctcccg gaccggcccc tcgaagatca tcagcggtcc accccgccca tcacgggatc
    12181 cacgctggcg atcgaggcga tcacgtcggc cacgagcccg ccccggacga tctcgggcag
    12241 cgcctggacg tggacgaacg aggggtcctt gatccggacg cggtaggggc gcaccccgcc
    12301 gtcggagacg acgtagaagc cgagctcccc ccgcggcgac tccacgcagg tgtagacctc
    12361 gcccgccggg accttcacgc cctgggtcac gagcttgaag tggttgatca gcgcctccat
    12421 cgactcctcc atgatgtggc gcacgtagcg ctcggagttg ccgatgccgt cggggccgac
    12481 ctcgagctgt gccggccagg cgaccttggg gtcgcccacc atcacgcggc cgccgggcag
    12541 cccgtccagg acctgctcca cgatcttcag cgactcgcgg atctcggcca cgcggacctc
    12601 gtagcgcgcg tagcagtctg cctcctcacg cgtggggatc tcgaagtcgt acccctcgta
    12661 gaggggatcg gtcgtccgga ggtcgtgcgg cacgccggcc gcccgcagga tcgggccggt
    12721 gacggcccac ctacggcact gctccgcggt gaggatcccc acgccgcggt tccgctccac
    12781 ccagatcggg ttgcccgaga gtaggctctc gtattcgtcc accttgccgt ccgtgtcggc
    12841 gaggagggcc cggatcctct cctcggcccc ctccgggagg tccatcacga gcccgccggg
    12901 gcggatgtac gcgtggttca tccgcaggcc cgtgatcgcc tcgaagacat cgaggagctt
    12961 ctcccgctct cggaacccgt acagcatcat cgagagggcc cccagctcca ggccggtcgt
    13021 ggccagccac acgaggtggc tggcgatgcg gccgagctcg cacaccatca ccctgatcgc
    13081 ctgcgctcgg ggcggggcct cgatcccgag cagccgctcc accgagagcg cgtaggccag
    13141 ctcgttggag aacggcgaga ggtagtcggc gcgcgtgacg aaggtggggc cctggtgcca
    13201 cgtccggtac tcagcgttct tctcgatgcc ggtgtgcagg tacccgacga cgggttggca
    13261 gtcgaccacc gtctcgccgt cgagcgtcag cacgagccga agcacgccgt gcgtggaggg
    13321 gtgctgggga cccatgttga tgaccatgag atcctcggcc aggcgctggg cctggacgcc
    13381 ctgggccatg tcggtccctc cgagcggggt ggggacgcgg tccactcaga gctcccgctg
    13441 gtccgggggc ggcacgtacg cgcccttgta ctgggtggcc acgccgccca gcggctcgtc
    13501 cttgcggagg ggatgcccga tccactcctc gggcagctcg atccggcgga ggtcgggatg
    13561 gccgtcgaac gtcacgccga agaagtcgta gacctcgcgc tcgtgccagc tcgccgaggg
    13621 gtggacctcg gtcgcggtga cgatccggag gtcgtcctcg gggagccccg ccttcacccg
    13681 gacccgcatc ccgtggcccg acgcgaggtg gtaggccatc cagatcctgg gctcgcgccc
    13741 cggccagtcg gtgcacgaga ggtccgagag gaagtcgaac ccgaggtcgg gttcgtcgcg
    13801 caggtaccgg agcgcgtccc cgagctcgtc gcggccgacc gtcaccgtca cctcgccccg
    13861 cgccacgacc acgtcgggga accgagagcc gagacgctcc gccagctcgg ccgggctcac
    13921 gtcagggtcc cccgctgctg gatcttctcg tggagctgga ggatgccgta catcagcatc
    13981 tcgggacggg gcggacagcc cggcacgtag acgtccaccg gcacgatggt atccacgccc
    14041 tggatcacgc cgtagttgtt gaacatgccg ccggacgagg cgcagacgcc catcgagatg
    14101 acccacttcg gctcgggcat ctggtcgtag atctggcgga gcaccggccc catcttctgg
    14161 ctgaccctgc cggccacgat catgaggtcg gcctggcggg gcgaggcccg gaagacctcc
    14221 atcccccacc gggccaggtc gtggtggccg gccccggtag ccatcatctc gatcgcgcag
    14281 caggccaggc cgaaggtgac cggccacgtg gactgcttcc tcgcccagtt gaccatcttg
    14341 gccagcgtgg tcgtgaggat gccctgacgg acctcctcct cctcggcccg cctgacggca
    14401 tccacaccgc gcgcggggtc gctcatcgtg cccactccag gccgtcccgc ttccagacgt
    14461 agacgtaggc cacgaacagg agcccgatga agatcaccat ctccacgagc ccgaagaggc
    14521 ccagctgccg gaaggcaacc gcccagggga acaggaagat cgtctcgatg tcgaagatga
    14581 tgaacagcat cgcgatcacg tagaacttca cggggaagcg ctcgggcggc tcgtgctccg
    14641 ggacgatccc gcactcgtac ggagccagct tggccggcgt gggcctccgc ggccgcagga
    14701 gggcggacgc ggcgagagag gccacggcga acagggccgc cagcccggcc aggatcacga
    14761 tcgggaggta ctcgaccagc atcccgatcg aggctagaag ctctcagagg acacgcgcaa
    14821 atccccctcg actcggcgcc agacgtgtca gcacgtggat cagacggtcg tcgaggtccc
    14881 cgtcccggcc gtccgtgagg ttcgccatca gcttgaagag gaaccgcatc agccgctctc
    14941 gggggaggcc gtaccggacg gcggcccgca tcacggtggg gctgccgatc gcccggacga
    15001 aggcccgccc catcgagaag tacttgccgt accgctggcg gagcaccgcc gggtagacct
    15061 gggccagccc cggccggtcg aggacgaggg cctcgtggat cagttccgcc gccagacgcc
    15121 ccgactccat ggcgtaggcg atcccctcac cgttgaaggg gttgatgatg ccgccggcgt
    15181 cgcccgtcac gagcagcccg gggacggcga gcggcgcgcg gttcatcccc atgggcagcg
    15241 ggcccgacag cagcctcccc tgggcggtct cctcggtgat cccgagctcg ggagggaggc
    15301 ggcggaggaa gacctcgtac accttcttgg cagagaggtg ccggaagtcg cgggccgtgt
    15361 tgagcaggcc cgcgccgacg ttcacgatcc cgtcccccac ggggaagatc cagccatagc
    15421 ccggcaggaa gccgccctga tgctcgagat cgagccagga gaggaagtgc cgctcgttcg
    15481 ccagggggct ccggaagtag cggcgcgccg cgatcccgat cggacgatcg ggatcgcggc
    15541 gcacccccgc ctgctgcgcg aaccgcgacg acgccccgtc cgcggcgatc acgaaccggg
    15601 cccggacctc gcgaggctcg ccctcgcccc tccgcacgac ggctccgagc acgcgccccc
    15661 atcggtccag gaccgggcgc tcggcgtccg tctcctgcca cacgaccgcc cccgacttct
    15721 cggcgttccg gatcaggagc tcgtcgagat cgtgcctggt ccgcacgagc ccgaagctgg
    15781 ggaaggcgtc cgactcgggc caggggagct cgaccttcac cccggggccg aaggacctca
    15841 ggccgtcgat gcgcacgaag ccccgctcct cgacatcgat ccccagctcg aggagctgct
    15901 tcacgccccg gggggtcagg ccgtcgccgc agaccttctc gcgggggaaa gccgccttgt
    15961 ccaccgcgag cacgtccacg ccgtgtcggg cgaggtgcgc cgcggccgcg gacccggcgg
    16021 ggccggcgcc cacgaccagc acgtccaccc gctcggcgga gcttgtgaaa ccgttcacaa
    16081 acggatgcta cgcccgcccc ccgtatctcg caaacgcctc agccggccgc gtccccggac
    16141 cgctcgccgg ggagccagag ggcatggtcc cacgccgcca tgtggagcgg gcggacccgc
    16201 atcatccccg gccgctccac ctgctcggcg aacgcccgga agccgtcccc cgacagcgcc
    16261 tcgcggtact tctcgtacca ggaggacatc acgccgcgga gcgcggggtg gacggccgag
    16321 accggttcgg cccggccgtg gatcacgatc cccgccaggt cccgccactc gctcccgcgg
    16381 ctgaaggtga gggcggcgcg cgggtcccgc tcgacgttgc gccaggtcac cgagtcgcgt
    16441 cgggccgaga cgtagatcgc cccctctcgc cacacgaacc agagcgggac gacgtggggg
    16501 ctcccgtcgg cgagggtggt cgcgaggtgg accacgtcga gatccccgaa catcctgcga
    16561 gcgtcgagca gccgcatggc ccggctagtt gtcccacacg cgggcgatcg aatcaatcac
    16621 ccggtcacga gatgcgcagg gtgcaggtct ccacgaggtc ggcgagcgcc gcgcgcacgt
    16681 cgccggggac ctcggcgacg tccagcgcgg cgcgagcccg cccggccagc tcggccacga
    16741 ggccgcccac cgcatcgagc gcccccgacg actcgatgat ccctcggatc cgctcgacgt
    16801 ccgcctcggt caggtcgggc gccccgagcc gctccaggag gaaggccgcc tccccccgct
    16861 ccgcgcgctc cagggtccgg gcgatcagga cggtccgctt cccctctcgg aggtccgagt
    16921 cccggggctt cccggtcagc gcggggtccc cgaagacccc gagcacgtcg tcgcggagct
    16981 ggaaggcctc gcccagcggc atcccgtacg cgctcagggc cgtcaagagc tcgccggagg
    17041 cgccggccag gatcgccccg atctggagcg ggccctccac cgtgtagttg ccggacttca
    17101 gccccgcgat gcgacgggcc tcgggctccg ccgcctcccc ccgatgcgcg gccgccacgt
    17161 cgaggtactg cccggccacg acctccaccc gcatccggtt gtagcgggcg agcgccggga
    17221 gcagcgctcc cggcgcgagc ccggcgccga ggaacgcgcc gtcggcgagg atcagcgcca
    17281 gatcgccggc caggatcgcc cccgacacgc cgaagacctc gccgctcccc cgcatccgct
    17341 cggcgcggtg gaggtcggcc agatgggccc aagtggcggg cgccccgcgc cgcgtccggg
    17401 acccgtccat cacgtcgtcg tgaacgatgg cgaaggtgtg gagcagctcc agcgaggccg
    17461 cggcccggat cacctcgggg cccacctcgc cccgcgccgc ccggtggccc aggaggcaga
    17521 agaccggccg gagccgcttc ccgccggcgg cgatcacccg ctccagctcg tcgaagagct
    17581 ctccgacgcc cggggcccgc tcctcggctt cggcccgctc gccctggagg aagcgggcca
    17641 ggtcctcctc gaccgcggcc gcgagctcga ggcacgccgc cggcaggggg acatcggcga
    17701 cgcgcacgcc cggcatcagc ccatcggggg gaggtcccgg aggaaggcct cgacctcctc
    17761 gcgggtggcc acgttgtccc agcgcccgcg gatcacgccc tcggcatcca cgaagaagac
    17821 ccagggctcg aagatgttgt cgttccggag gatccagtcg gcggcggccc ggttcaccac
    17881 gttgttctgg aagtctcgcc acacctcgac gtggacgaac tcggcccgat cgcggtactc
    17941 ggactcgagc tcggcgatca tgtccgtgat cggaccgcag aactggctca cgcagtagac
    18001 gggggtggcg aacacggcca cgacgggccg cccccgccgg atcgcctccg cgatcgtcga
    18061 gtcgtggagc tccgggtcgg ggacctcctc gcccccctcg gccctcgagt cgatggcccc
    18121 cggaggagcc tccccgatgt cgtcgagcgt gaggttgtcg gtccggggcg cccggtcgcc
    18181 gacgaccggg tacgcatgct cgccagccac ctggaaggcc gccgtccccg agagcggctc
    18241 gccgtcgacg tcggcggtca cctcgacctg ccagatgccg gcgcggtcga aggtcacctc
    18301 tcgggccgcg tagacgcccc ggccctcggc cggcgggccg gcgtgggctt cctcgtgctc
    18361 ggggtcctcg ccggggatgg ccaggaactc cgcggtgacc tctggacccg gctcgggctg
    18421 ccctcccccg atgaaggaga agcggaaggt cgcctcgccg cccgagacga agcggttgtc
    18481 ctgggtgagg aggcccacga tgaaccggga ggcggtgccg gtggcgaggt cgtagctcgc
    18541 gacctgggcc acgagctcgc ctcctccggg gctcggcgaa gggccggcct gcggcccctc
    18601 gtctccctcc gacccgcagg ccgcggagac caggcccagg gcgagcgcca gcgcgagcgt
    18661 cgtggttcgg gtcacggtcc cccaatgtag cgcgggcggc gtgggtccgc ggcggcgtga
    18721 gtatgatgag ggcacggatg aagctcgttc gccgcctctc cgtcgtcacc gtcatcgcca
    18781 cgctgggact ggtcgcgctg ggcggcgtgg ttcgggccac cggctcgggg ctgggatgcc
    18841 ccgactggcc ccggtgccac gggcggctga tcccgcccct cgagttccac gcgctgatcg
    18901 agtacagcca tcgactgctg gcctcgatcg tcgtgatcct cgtcatcgcc accgcgtggg
    18961 ccgcgtggcg ccgggcccgt gggacgcctc aggtcctgcg gccggccgtg ctggcgatcg
    19021 gcgtggtcct gctccaggcg ggcctcggcg cggtggtcgt ggccgaggag ctggcccccc
    19081 tccccaacac gctccacttc gccacggcga tgctgctcgt ggccacggtc gtcacgaccg
    19141 cggcgaacgc gacgctgggg cgcgcggcgc gcggcgaggc tccccgcgcc acgccgggca
    19201 tgcggcgcct gctgtggtgg gcgctcggcg tcacggcggc cgcgctgctg accggcgtgt
    19261 acatgcgcgg ctcgggctcg agcctcgcct tcctggactg gccgctgatg gacgggaggg
    19321 cgatccccga gctccggacc gagtcggcct gggccgcgtt ccttcaccgg gcgatcgccg
    19381 cgctggccgt ggtcctgatc ctcggggtcg ccgtgcgggc ccgcgagctc gccccgcgga
    19441 tccggtggct cgcccggacg gccaccgggc tggtcttgct ccagtcggcc ctgggggcag
    19501 ccacggtctt cacccggctg gacgcgctgc ctgtggccgg ccacatcctc ggcagcagcc
    19561 tcacgtgggc ctccctcgtg gccctcgtcg ccggcacccg cccgctgcgg cacccggagc
    19621 cggtcgaaga ggccgaccgg cccggcgcgc ggcaggtcgc gggggcctac ctccagctga
    19681 tgaagccgga catcatcgtg ctcctgctcg tcacgaccat cccgacgatg atcctcgcgg
    19741 caggaggcct cccgcccgtg ggcctcgtgg ccgccacgct cctcgggggc acgctggcgg
    19801 cggggggcgc caacgcgctc aaccacttcc tcgaccggga catcgacgag gtgatggacc
    19861 ggacccgggc ccgccccatc cccgcgcacc gggtggagcc cgacgacgcg aagagcttcg
    19921 ggatcctcct ctcggggctc tccttcgcct ggctggcgct gacggtcaac ttgttgtcgg
    19981 cggcgctggc cctctcggcg atcctcttct acgtgctcgt ctacacggcg tggatgaagc
    20041 ggtccacgcc gcagaacatc gtgatcggcg gggcggcggg agcggtgccg gccctcgtgg
    20101 ggtgggcggc ggtgaccggg accctggccc tgcctgcgtg ggtcctcttc gcggtgatct
    20161 tcttctggac gccgccccac ttctgggcgc tgtcgatgaa gtacgcagac gactaccagg
    20221 cggccggcgt gccgatgctc gtcgtcctcc acggcagcgc cgacacggcg acccacatcc
    20281 tcctgtactc gatccagacg gtggcggtgt cgctgctgct cttcccggcg gcggggatgg
    20341 gaacgctcta cctcgccgcg gccgtcggcc tgggcgggat cctgatctgg tacgcggcgc
    20401 gggtcgtccg cgagcggacg acccgcgccg cgatgcacct ctttcactac tcgatcacct
    20461 acctcggcct gctcttcgcc gccgtcgccg ccgacacgct gatcggggcc ctggcgtgat
    20521 cgagctcttc ggcgacgtga tcaggaacca tgggtggcac gtcgtggccg agctcgtcct
    20581 gctggcgctg gccacggtct tcaccgcctt ctacgtcgcg gccctggtcc ggggcagggt
    20641 ccgagccgtc gagtgcgcgg gctgcggcag gatcgcctcc cgggcccata ccgagtgccc
    20701 ccgatgccgg accccgctcc cgacgtcgtg agcttcggcc ctccgacgac cccgtgctat
    20761 ggtgtcgccc gttcggggcc cgatcacgcc ccgccgcatc cgagatgagg tccgaccgag
    20821 cgcgccgcac gccgagcacc cgccgacgct ggtggctgac gggcttcgtg gtcgtcaccg
    20881 ggctgctgct cgccgcctgc cagcaggggc tgccccagtc ctcgctcgag cccgccgggc
    20941 cgcgcgccga acgcatcgac gacctctcca cgatcgtctt ctggatggcc ggcgtcgtct
    21001 tcgtcctcgt ggagggagcg atcatcttca tcacgatccg ctaccgccgc cgccgccgcg
    21061 aggatcaggc gcccgtgcag atccacggca acaaccgcct ggagatcatc tggacggtca
    21121 tcccggccct cctgctggcc ggcctggcct tccccaccgt cgccgcgatc ttcgacctct
    21181 cgcggcggcc ccccggcggg gacgtcctgg aggtcaacgt gatcgggcac caatggtggt
    21241 gggagttccg ctactccgac ctgaacatcg tgacggcgaa cgagctcgtg atcccggtgg
    21301 acacgcccgt gcacctgagc ttggagtcgg ccgagacccc gggcggcatc gccgagttca
    21361 ccgccgacgg ccagcccgtc acgcagaacg cgatccccgt gatccacagc ttctgggtgc
    21421 cgaggctcgc cgggaagcag gacctcgtcc ccggcaaggt cgagacgctc acgatcgagg
    21481 cgtccgagac cggcacgttc ctgggccagt gcgccgagta ctgcggcacc tcccacgcga
    21541 acatgcggaa gcgcgtgatc gtcgagacct cggacgggtt ccaggagtgg gtctcgcgga
    21601 tgcaggcgcc ggcggcggag cccgagccgg gcagcctcgc cgcccagggg gcccagttct
    21661 tccagaactt cgggaccggc tcctgcctgg cctgccacgg cgttgagccc ggagccggcg
    21721 gcaccgttgg ccccaacctc gcccacctgg ccagccgccg gacgttcgcc gcgggcctgt
    21781 tcgagacgaa ccgggagaac ctccagcggt ggctggacaa cccccgggcg atgaagcccg
    21841 gggtggtgat gcccgactac aacctgaccg aggagcagat cgaggcgatc acagagtatc
    21901 tgctgacgct cgaatgacgc gagaggtgaa cgactgatgg cgatcgcgca gccgcttccc
    21961 cggggctcgg tgttccggcg ccccacggcc acgaccgggt tcgtgtcgtg gctgacgacc
    22021 gtggaccaca agcggatcgg gatcctctac ggcgtcacgt cgttcgcgtt cttcctgctg
    22081 ggcgggatcg aggccctgct gatccgctgg cagctcgcca cgcccgaggg gcagctgctc
    22141 gacgccgacg tctacaacca gctcttcacg atgcacggga tcacgatgat cttcctcgtc
    22201 gtgatgcccc tctcctccgc gttcttcaac tacctcgtcc cgctgatgat cggggctcgg
    22261 gacgtggcct tcccccggct caacgcgttc agctactgga tcttcctgct gggagggctg
    22321 ttcctctact cgtcctggtt cctgggcggt gccccggacg gaggatggtt cgcgtacgcc
    22381 cccctctcca gccagatgcc ggggaacaac ctggacttct acgcggtggg gctccagatc
    22441 gtcggcgtgg cctcgctcgt ctccgcggtg aacttcatcg tgacgatctt caacgagcgg
    22501 gcgcccggga tgagcctgat gcggatgccg gtgttcgtgt ggatgacact cgtcgtctcg
    22561 ttcctcctgc tgttcgccct gccggtgatc gcgatcgcgc tgttccagct ggccggggac
    22621 cgcatcctgg gcacgcactt cttcgacgcg gcggccgggg gcgacccgat cctgtggcag
    22681 cacatgttct ggatcttcgg ccaccccgag gtctacatcc tgatcctgcc cgccatgggg
    22741 atcgtctcgg agatcctccc caccttctcg cggaagcctc tgttcgggta tccggcggtg
    22801 gtgttcgcag gcgccgcgat cgggttcatg gggtgggggg tgtgggccca ccacatgttc
    22861 acctcggggc tcggcccggt ggccaactcc gccttcgccc tctccacgat gttcatcgcc
    22921 gtccccaccg gcatcaagat cttcaactgg ctggggacga tgtggggagg ggccatccgg
    22981 ttcaccacgc ccatgctgat ggcgatcggt ttcgtcgcca tgttcgtgat cggggggctg
    23041 tccggggtca cgcactcgat cgtgcccgcg gactggcagc agcacgacac ctactacatc
    23101 gtggcccact tccactacgt gctgttcggc ggcgcgatct tcgggctgtt cggaggggcc
    23161 tactactggt tcccgaagtt caccggccgg acctacagcg agcggctggg ccacctccac
    23221 ttctggctga tgctgatcgg attcaacctg acgttcgcgc cgatgcacct cctgggcctc
    23281 gaggggatgc cccgccgcat ctacacctac ggcgagggtc tggggttcga cgtctggaac
    23341 atggtctcca cgatcggcgc gttcacgatc gcggcctcga tcctcgtgtt catggtgaac
    23401 gtggtgcgca gcctctctcg cggcgagaag gcgccgcccg acccctggga cgggcggacc
    23461 ctggagtgga cgatcccctc cccgccgccg cactacaact tcgccgaggt cccccaggtc
    23521 cgggcgcggg acgacttctg gcaccggaag tacaccgagg acgggtccgg gcgggtccag
    23581 cccgtcgtgg ccggcgcggc gaccgaggag gccgaggagg aggagcacgc ggaggaggag
    23641 cacctcgaca tccacatgcc cgatccgtcg tactggccgc tcgtggccgc ggcggggctt
    23701 ccgttcctcg ggtacggcct gatctacgac tggatctggg ccgtgccggg cgcggcgctc
    23761 ctgctgttcg ggctcttcgg atggagcctg gaaccggtgg ccgaggaggg gcactgatgg
    23821 cgcaggccca ggtccgccac gaggctcaca cggccaccgg gctcgacaac cggaagctcg
    23881 ggatgtgggc cttcctcggc tcggagtgcc tcttcttcgg ggcgctcatc accacgtacc
    23941 tcctctaccg gggccgcagc gtccaggggc cgttccccga ggacatctac gacatcccct
    24001 tcacctcggt gagctcgttc gtcctgctga tgagctccct gacgatggtg ctggcgctgt
    24061 cggcgatcca gcggggcgac caccagggcc tccggatctg gctgctggcc accgcgttcc
    24121 tggggatgac cttcctggga ggccaggtct acgagttcac cgtcttcgtc aacgagggcc
    24181 tgaccgtcac gaggaacgtc ttcggcagcg cgttcttcgt gctgaccggc ttccacgggc
    24241 tccacgtgac ggtcggggtg ctgatgctgc tgcaccactt cggtatgtcg gtggccgggc
    24301 ggatcgggca ggaggacagc ctccggatcg agctcgtggg cctctactgg cacttcgtgg
    24361 acatcgtctg ggtgatcatc ttcaccgtcg tctacctgat cccgagatga ccgaggacgc
    24421 gcacgtcgca ggccggaccg aggaggatcc ccgcacgcac gcgcgggccc tggagcatgc
    24481 ccaccccgcg ccggccgagt acgtgaaggt cgccgtggcg ctggcggtcg tcacggccct
    24541 cgaggtgggc ctcttctaca tcgagggcct cgccgacggg ctcctgatcg ccctcctgat
    24601 ggtgctgatg gcgatcaagt tcgccatggt cgccatgtgg ttcatgcacc tgaagttcga
    24661 caacaagatc ttctctcgcc tgttcatcac gggcatcctc ctggcgatcg gcgtcttcac
    24721 cgtggtgctc gtgacgatcg gggtggtggg cgactgatgc cggcctggca cccgcacccc
    24781 gacgcctggc tgctcgtcgc cgtgatcgtc gccacctacg tgtacggcct ccgccgcgtc
    24841 ggcccccgcc acgtctcgcc gggggatccg ccggcgagcc gaggccagct gatcgcgttc
    24901 gggctcggag tccttgccct cctgatttcg gccgactggc cgatccacga cctcggcgag
    24961 cggtccctct tcaccgcgca catgctccag cacctgctgc tgacgctcgt agccccgccc
    25021 ctcctcctcc tcgggacccc cggctggatg ctgcggtggc tgctccgccc tcggtggctg
    25081 ctctcgctgg tccgcgcgct gtcgaagccg ctcgtggcac tggtgctctt caacctggtg
    25141 atcgccgtga tccactggcc ggccttcgtg tcgctgtacc tgcgggagcc gctcgtccac
    25201 ctcgccacgc acgcggcgct cctggggacg gcgctgctga tgtggatgcc ggtcctctcc
    25261 ccgatcgtcg agatcgcgcg actgtcgtac ccgctgcaga tgctctacct gttcgcgcag
    25321 tcgatcgtcc cgacggtgcc ggcctcgttc ctgacgttcg ggagcgagcc cctctaccgg
    25381 atctacgagg agctgccccg cctctggggg atctcggccc tcaccgacca gcgggtggcg
    25441 gggctgacca tgaagctgct gggcggcctc atcctctggg gcgtgatcac gatcctcttc
    25501 ttccggtggg cgaagcagga ggaggagggc gtggacgacc tcggctggcg ggacgtcgag
    25561 gaggccgtca acccgaagga gctccttacc cgatgagcga ggaccggcgg cgccgctccc
    25621 tgctcgcgat cttcgtgccc ctgacggccg tcctggcgat cgcgatcgtg ctctggtcga
    25681 tgtcgcggat cctcctggcg gcctcggtga acgtcgcccc cgtcatcgcg ctgctcttcg
    25741 cgctgaacat cctgatcgcg gcgacgctcg cggccacctt ccgtcggagg acgggcgtcg
    25801 tcctggcgtt cgtcatggcc gtgctggtcc ccgtgctggc cgtggggatc gtgggcgcgg
    25861 tggccggcga gcgcgagttc cattcggcga tcgaggagga gcccgagccc ggcccgagcc
    25921 ccaccgcgga gcccaccgag gagcccaccg aagagccaac ggaggagccc accggaccgt
    25981 ccgagggacc caccgaggcc acgatcgtgg ccctcaacat ccagttcgat accgacgcca
    26041 tgcagctcgc ggcgaacgcc gaggtcacgg tgacgctcga caaccaggac gcgggggtcc
    26101 cccacaactg ggcgatgtac acggacgagt cggcgagcca ggagatcttc gtgggcgaga
    26161 tcatcaccgg gcccgagacg atagactact cgttcacgtc tccggatccc ggcgagtact
    26221 acttccactg cgacgtccac cccaacatgc gggggaccgt caccgtcggc tgatcacacc
    26281 ccggcgtacg agtgcaggcc ggagacgacg aggttcacca cggtcaggtt gaacgccagc
    26341 gcggcgaacc ccaggacggc cagcacggcc gctcgcctcc cccgccagcc ctgcgtcgct
    26401 cgcgcgtgca ggtagcccgc gaagagcacc caggtgacga acgaccagac ctccttgggg
    26461 tcccagcccc agtagcggcc ccaggcctgc tcggcccaga tcgccccggc gatgacggcg
    26521 aacgtccaga tcgggaaggc gaacgtgatc gccttcgctg ccagatcgtc cagcgcggcg
    26581 gcttcgggga gcctgccccg gcgagtcggc ccctcccgca tcccgccgtg cccgttcccc
    26641 tgctcggggc cgagcgtcac ggtggcccgc ccctcttcgg ggtcggcctc gacctcgagg
    26701 tcatgcggca ggccggagcc caccgacgcc atcgccggcg ccggacgctc agccacggcc
    26761 cgctccaccc gccgctcccg ccgctcctgg atcaggtaga gcaccgtgaa gacgaacccg
    26821 agcgtgaaga gagccgaggc caggatcgcc gccagcacgt ggatccgcag ccaccccgag
    26881 ttgagcgccg ggatcagcgg gccgggctcg acgtagacca tcgtggaggc ggccatgatc
    26941 agcaccgtgg ccccaaggac gaagccgccc agccgccggg cgtccacccg ccgctcggcg
    27001 acgacgaggt acagcgccac gacggccaag cccacgacgc tcgcgtactc gtacatgttc
    27061 ccccagggga cccgctccgc ggccacaccc cggaggacga cggagaccgt gtgcgaggcg
    27121 agccccaggt aggccacgac cctggccacg gtcagcggcg tgggtcgccg gtacgcgaag
    27181 tgatggaggt acccgaccat cgccaccagg tagagggcca gcgcgacccc gaacgtcgtg
    27241 tttgaggcct cggtcatgga acccccactc taccgcgggg caccaggtcg cgaacgagac
    27301 gatcgaactc ctcgtcgaag gccgccttcc gctggagggc gaagcctccg atctcgagcc
    27361 gggtgccgcc gtcctcgggc actgcccgca cccagatccg gcgacggctc gagtagaggg
    27421 ccgggacaag gccgacgagg acgagcaggg cggtggccag catcagcccg atccccgggt
    27481 cgcgcttcac caggagctgg gtgtaccggc gcagatcgcg gaaggtgacc tcgaggccgc
    27541 cgggaagctc gaccgtctcc cccagcgcca gaccgccgct gtcgtactcc tcgagcagct
    27601 ccttctccag gcggaatacc gactgggcct cggtcagccg gaggtcgcct cggtacgcgc
    27661 ggtaggcgat gaagggatcg tccgcctcca gcatgggcat ccccgtcacg aacgcagcgg
    27721 cgtccgggag gagctggagc tcgagcccga cctggggcga cagcgcgggc agcttgatcg
    27781 ccccccgcca ggggcggacc gggtcgttcg ggtcgtcggt gacgaacacc agcgggtccg
    27841 agacgagcag ctcgccctcg tgccgcacct cgaccacggg cgcccagccg tagcccgact
    27901 gatagagctt cacgccgcgg tgctccagcg ggtcgttgac ccggatctcg tccgtgcgga
    27961 ccacctcgcc ctcctccacg acgtccaccc gcgagacgaa gtcccgcggc accccgccct
    28021 cgcggtacgt cacctcgaag tcgagcacgc ggacctggaa cccttggtgc ctctcgccga
    28081 agaaccgccc ctcgcgcgga ggaaagtcgt agctcgcgtg ggcctcggtg aagacgtccc
    28141 cctcgatcac cgtggcctgg cccgtgaagc cgaatccctt cccgtacacc accccgatca
    28201 gcaggaggaa gaaggcccag tggaaggcga ggctgcccac ctcccgggcc aggcccttct
    28261 cggcggccag cgccgtctcg cctccgctcc gcacacccca gccccggcgc cggagcgccc
    28321 gccgggcacg gcccacggcc tcctcgggcg acccgtcgac gctgccgctc gcgtagtgcc
    28381 ggagcgcctc gaggtcgcgg gccggctgcc accgcgagcc gagcccccgg atcaggccgc
    28441 gcgtccgcgg gatcaggcac gccgccagcg agatcagcag gaggacgtag aggagcgtga
    28501 accaccacga gccgaagacg tcgaagaagc ccagcgcgtc gtagatccgg gcgagcagag
    28561 gccggtcggc gaagagctcg gccacgcggg ccggcgagtt gggcgcctgg ggaacgagcg
    28621 acccgaccac gctccccagc cccagcagga ggaggaggat cagcgccgtc cgcatcgagc
    28681 ggagcgagcg ccacaccgag agcatgccgc gcgagaaggt cccggtcgcc atcgcccccg
    28741 atcgtacgcg ggcgccgcta gatcgggggc tcgaagccgc tgacgaggtc gaggaggccc
    28801 gaccaggcca ggaagcgcgt ccagaggttg agcatcagca ggacgccgat gaccaccatc
    28861 agcacgcccc cgatccccgc gatccagtgg tagttgcgct tcacccaggc cagggcgccc
    28921 atgaggcgtc gcagccccag gcccacgagg acgaagggca gccccagccc gagcgagtag
    28981 gtgaacatca gcagcgcccc ccacgccccg ccgccctggg ccgccgcgat cgtcaggatc
    29041 cccgccagca ccgggccgat gcacggagtg aaccccgtgg cgaaggccat ccccagcggg
    29101 aaggccccgg cggtccccgg ccgaaccttc gacaggaacg ggcgccgctc cgcgaagagg
    29161 cgaggcccgc cgatcctgag cgagtacagg atcaggtaga ccccgaacag gacgacgatg
    29221 cccccggcga tccgctggcc ccacgggtgg cggaagatct ccgcgaagac ccgcgagaac
    29281 gcgccgagga gggtgaagac gagcgcgaac cccgcgacga agagcagcat cgggagggcg
    29341 gcccgcgtcg ccggccgctc ggccaccgcc accgcgccgt ccccggacgg cgacgcgggc
    29401 cgcccgtccg ggccctggcc gagcacgaac ccgacgtagc cgggcacgag aggcagcacg
    29461 cacggcgagg cgaacgacac cacgccggcc gcgaaggcca gcgccggcgc ccaccagacg
    29521 ttcaggtcca cgcttcctcg tcccctcggt tgcgaacggc tcccagtatg cgcatcattc
    29581 ggggtgaagg acttgtgaaa ggattcacaa gccgtgcgag cggggggcct cgcgcggcca
    29641 gggtttgggg aggcgggagc cgcacgatag aatcggcccg ccccgaggag tggaggctga
    29701 gtgagactgc cgatcccgag cagcgtcagg cgcaggatgg agccctacaa ggagcgcgcc
    29761 gacgacttcc tcaggcgatg ggagtggacg tggacgtccg ccttcatcgc cgggctgatc
    29821 atctcgttca tcgccatgac gacgctggtg gtgatcccct cctggttcct cttcttcgcc
    29881 aaccagaccc tggggtggtc gaccgccgac cgcctgctcg tcgtgatccg cgactcgatc
    29941 acgatgggct ggatcaccgt gtggacggtg gccttcatct tcgtcgccta caaggtccag
    30001 gtgcaccgcc gcaggctccg tggcgagcgt caggccgaac ggtactctgg gggctaccga
    30061 tagtgcgagg ggtgagctga cgtggccttc cagaagaccg aggtcaacat ccacgaggac
    30121 tacacgatca cgcccgtgga ccccgagtgg ctgaaggaag gggtgaagtc cccgaagcac
    30181 ctgatgctcg acccggatcc ctgcatcctg tgccgggcct gcgaggacgt ctgtccctgg
    30241 aactgcatct acatgctcga cccggggatc gtggagaacc ccggc
//
LOCUS       DASUTZ010000272        12450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_366956, whole genome shotgun sequence.
ACCESSION   DASUTZ010000272 DASUTZ010000000
VERSION     DASUTZ010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12450
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366956"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VF097_11775"
     CDS             <1..559
                     /locus_tag="VF097_11775"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX6263514.1"
                     /translation="VRKEGVMETIQRETNAAPPPVATRLTSEEVWRSVDKASFAVLGY
                     VTPTGEPRSSGVVYKALGRRLYVATAVDSWKARHIAASGRVSVTVPVRRGGLLSLVVP
                     IPPATVSFHGTAIVHSAGAPEVASILEELGSLLPRERRDSAAIIEIEPEGMFVTYGVG
                     VSLTRMRDPVAARARAPVSPGGVPS"
     gene            670..1338
                     /locus_tag="VF097_11780"
     CDS             670..1338
                     /locus_tag="VF097_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF domain-containing protein"
                     /protein_id="HEX6263515.1"
                     /translation="MRPIRLWIGAALVVLGVLAVLDATGVLESTGVVERWWPIAVIGT
                     GVLGMVLRRRLTLGMLVITAIGFALLADAQDWTTEDVLGPALLVIIGLAVLAPFWSRG
                     RTTASDTEGESPFALFGAAKVKDHSEHLQHAEASALFGGATLDLREAHIEDEATVEAT
                     AIFGGVDVLVPRDVRVSLGGLPIFGGLEDKTTGDGELPEDAPHLNVRAMAIFGGVDVA
                     HEPD"
     gene            1415..2821
                     /locus_tag="VF097_11785"
     CDS             1415..2821
                     /locus_tag="VF097_11785"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6263516.1"
                     /translation="MAASAALVALLSVSSACAPGSPESVDLELRPCTIGGVEAECGRL
                     SVPENPHAPDGPRIRISVALVRARESDPAPDPLFYLAGWGSAGIEDDMGAAAPMLGEI
                     NEQRDLVFIDQRGTGGSNRLDCSVPTEREIERTGLRAYTEAARTCAEEIGPNLRYYTS
                     SVAVDDVDRVREALGYERINIYGGSYGVTLGLIYLQRHGSHVRSAVFDSGSLLDVHIF
                     ERAAVGGQRALEMLFDRCEADPRCGMVFPDLRDEWERLAARVESRSIAVPGRQERLDP
                     IMLAGLVEELLSRTPTKTILPRVIHLLATGEIETVAEMFPPTSPPTEEPAYMALIQCS
                     EPWASRRPPHVARLSEGTFMEPLLRRAAAITQATCRALPRGEAPAEIGRRVRSDVPVL
                     FLTGDEDPADPPANVARARRELPNSLTVVFPEAGHGQLELPCAQDLVSEFVRAGSSEG
                     LNTSCAATAALLPFDTRT"
     gene            3192..5054
                     /locus_tag="VF097_11790"
     CDS             3192..5054
                     /locus_tag="VF097_11790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX6263517.1"
                     /translation="MPAQHDLRGLLREVHDRARLNWDALSTMGRLSISGVCVSSLLVI
                     GLGVLLPRVFLGHALGARVDAVHSMVHALESQGLVPAPDVPLTGEAYERFDQVVRGGL
                     LGGENAHVKLWTPSGEVVYSDTGSEVGREHATSRGMQDALEGQPFAQIRDLSTREPGP
                     EHDRPSRAVVLYVPVHQEGSRPIGVFEVHQDFEALSRRLALVRGAVWAAIGFGLFVLL
                     ALLASLFAGTARVMEREKRDAQERAEDLSLLLGTSRSLSSEPALTASASQVLTTLTER
                     LGLRCAAILTDEEGSGSAVTTAGRDALCSAALELARRTCEGGSGNDRLVRGDPGLGHG
                     APDHRCAVLGIPFQARQDACGAIVVCRDADDPFADRERTLISAVAGQLGFAARNDRLL
                     RGLREMSAVRGRLLRRLVHAQEEERRHLIGDLHDGLGQSLTRVLQGLRGSRARLPRTE
                     PAIQAELERLESLVDEQSRGLRGYLRTIRPAVLEDFGLAAALEAFAREQESESGIRIA
                     FSLDHLSDLDPPTELVLYRAAQEAIMNARKHSGARRLWLRLKPSETDAVLEVEDDGRG
                     ATEIRDGMGLASMRDRVASLGGTVEVHSSPGRGTRVRVLIPMEAEHGAHQDVRR"
     gene            5026..5667
                     /locus_tag="VF097_11795"
     CDS             5026..5667
                     /locus_tag="VF097_11795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6263518.1"
                     /translation="MEPIRTFVVDDHEIAREGLRWMIGADATIQVVGEAETASEAMQA
                     IVRSLPDVVLLDIHLPDRSGLEVLREMQERFPDLPVVILSMEDDPEYVEQAVRAGAAG
                     YLVKNASRDEMVRAVRAAHAGGGYIQSEVTRPLLARFAREVRAHGDAPHLSPRELEAV
                     SLLAEGLTNRQIADRLGISESTAKGYLRQVYERLGAADRAHAVAIALRSRIID"
     gene            5811..6341
                     /locus_tag="VF097_11800"
     CDS             5811..6341
                     /locus_tag="VF097_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263519.1"
                     /translation="MTYRTFKWIYGILASALALSFFGHGAWAVFENKESFRELLSGTM
                     NNVFGASTSIDDGAISAAVRAIGWWDIVFTVGILALAYGVIKGHGALFRVASSPVALF
                     AYSWALLWGFLTAGSRVTAAGVFYPEVWDVVERGPNFIVPVAMLYMTYLLRRPSQLIE
                     REEVPAEEKLQEAPTV"
     gene            7182..7649
                     /locus_tag="VF097_11805"
     CDS             7182..7649
                     /locus_tag="VF097_11805"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEX6263520.1"
                     /translation="MELSEWLSFIHVLTAMLWVGTGVALNVATSRVVASGDDERVVQF
                     TGDVEFLGNWLIGPSAAAVVGTGIWITAIEDWVAFSQLWIILALVGVAVSMLLGMGYF
                     GPEGRRLGRLAGERGASDPDLKTRTRRLLLVSRLDILVLVFLTWDMVFKPGLE"
     gene            complement(7689..8225)
                     /locus_tag="VF097_11810"
     CDS             complement(7689..8225)
                     /locus_tag="VF097_11810"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6263521.1"
                     /translation="MTHLRLETERLVLRPLTLRDLDDMASLLGDAEALTFWGSPLDRE
                     GARDWIQRNLHRYETDGVGRCAVVLRATGELIGDCGLIRTLVEGSQETELGWIVRRSH
                     WGKGIATEAGAAWRDHAFDNLGIDRIVSMISEANAASRRVAEKLGMTIERKAMWGGLP
                     HLMYSLRPGVLDRRTDQR"
     gene            complement(8501..9175)
                     /locus_tag="VF097_11815"
     CDS             complement(8501..9175)
                     /locus_tag="VF097_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6263522.1"
                     /translation="MVGRIFAVAEAQARDLARRRAAMALLVLLPLAFYLSTPSEDEFA
                     LVAGGTGMAWAVAGGALFLTLASRRIDSRLVLAGYRPREVLVGRLLLLEGLALVLVVL
                     FSALMVGVSSPPEPGALVLAVALSGLVAVPLGLAVAAVFPRELEGALTLIAVVGIEMG
                     LPLDSGLAPFLPLWGPLKLVEVARGAAEGLALPTLHALASTVVLLVISAALWWRRVRV
                     TRHEAL"
     gene            complement(9175..9747)
                     /locus_tag="VF097_11820"
     CDS             complement(9175..9747)
                     /locus_tag="VF097_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018569834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6263523.1"
                     /translation="MNLDLRPGEAVALVGENGAGKTTLLRICAGLLRADSGTVHVPGR
                     IGYCPQDAGVLELLTADEHMVLFGRAAGLSRQTALREGRRSLAWFGFPARERAVAKDL
                     SGGTRQKLNLALALLGDPEVLLLDEPYQGFDRGTYVNFWDHVDDWRSAGKAVLVVTHM
                     MAELTRVDRVVELPVHATGGREPEPAGGPV"
     gene            10069..10963
                     /locus_tag="VF097_11825"
                     /pseudo
     CDS             10069..10963
                     /locus_tag="VF097_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670052.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            10960..12147
                     /locus_tag="VF097_11830"
     CDS             10960..12147
                     /locus_tag="VF097_11830"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6263524.1"
                     /translation="MSGGWRSLWLVARREWDQRVRSLSFRVSTLLSILIVVALIAVPE
                     MYGGGAEPTHTVGLVGDSGAELPASLRSAGEEMGLSVKVREFADESSGRAALRSEDIS
                     VLLVDQRDLVWRAEVDQGLRAAVTSAVQALDRREAIAELGLTSEEAGRLIGPLGLRST
                     SLEPAAAERSARRDLGFVAVVMLFMAIAFYGGFVLVGVVEEKSSRVVEVLLARIRPAQ
                     LLGGKILGIGLVGLAQFALVVGSALVAVSLSGNTLLPSTTPSTLGSILFWFVTGYAFY
                     SVLFATAGSLISRQEEAQSVQFPVTAFLLVGYVVAFQAAAAPEGVAALVGSLLPPTAP
                     MVMTVLMAQGGVAWWHVALSAALLMATTYGMVLLAGRLYSGAVLRLGRRVRLREAWHG
                     AEG"
     gene            complement(12210..>12450)
                     /locus_tag="VF097_11835"
     CDS             complement(12210..>12450)
                     /locus_tag="VF097_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263525.1"
                     /translation="PKVAVPIRIGGGASDITVIRPEGVPIRVRTGGGSSNLTIDDLRF
                     ASAGKTDWQSPDYEGAEARYDIEIGAGTSAFTVRT"
BASE COUNT         1834 a         4097 c         4530 g         1989 t
ORIGIN      
        1 ggttcggaag gagggcgtca tggagacgat ccagagggag acgaacgcag ctcctccgcc
       61 ggtcgcgacc cgactcacga gcgaggaagt ctggcggagc gtcgacaagg cgtcgttcgc
      121 ggtgctcggc tacgtcacgc cgacggggga gcctcgatcg agcggcgtgg tctacaaggc
      181 gctcgggcgg cgtctctacg tcgcgaccgc ggtggacagc tggaaggcgc ggcacatcgc
      241 agccagcgga agggtctcgg tgacggtgcc ggtgcgccga ggggggctgc tctccctcgt
      301 cgtccccatc cctcccgcga ccgtcagctt ccacggaacg gcgatcgtgc actccgcggg
      361 ggcacctgag gtcgcgtcga tcctcgagga gctgggctcc ctcctcccgc gggagcgacg
      421 ggactccgcg gccatcatcg agatcgagcc cgaggggatg ttcgtcacgt acggcgtcgg
      481 ggtctcgctg acgaggatgc gcgatccggt ggcggcgcgg gcgcgtgcac cggtgagccc
      541 gggaggcgtg ccgagctgac cagcgggcct ggatggggca agagggctcc aggagagggg
      601 gtcgtgggcc tctagtccga gcctcccacg ggtgccaccg tgtggttgcg gcaagggaag
      661 gaggtcgaca tgagacccat acggttgtgg atcggggccg cgctggtcgt gctcggggtg
      721 ctcgccgtcc tggacgccac gggcgtgctc gagtcgaccg gggtcgtgga gcgctggtgg
      781 ccgatcgcgg tcatcgggac gggcgtcctg gggatggtcc ttcggcggcg cctgaccctc
      841 ggcatgctcg tcatcacggc gatcggtttc gctctgctgg cggacgcgca ggactggacc
      901 accgaggacg tgctcggccc ggcgctgctg gtcatcatcg ggctcgccgt cctggcgccg
      961 ttctggagtc gagggcggac gacggcctcg gacaccgagg gagagagccc gttcgccctg
     1021 ttcggggcgg ccaaggtcaa ggaccactcc gagcatctgc agcacgcgga ggcgtcggcg
     1081 ctgttcggag gcgccacgct cgacctccgc gaggcgcata tcgaggatga ggccacggtc
     1141 gaggcgaccg cgatcttcgg gggcgtggac gtcctggtcc ccagggacgt acgcgtgtct
     1201 ctgggcgggc ttccgatctt cgggggcctc gaggacaaga cgaccggcga cggggagctg
     1261 cccgaggacg ctccgcacct gaacgtgcgg gccatggcca tcttcggcgg ggtcgacgtg
     1321 gcccacgaac ccgactgagg gccctccgat gagcgacggc cgacgcggcc gagcgcctcg
     1381 gttacgagcc ccgccgggta tcggaccgag gcgcttggcc gcgtcggccg ccctcgtggc
     1441 gctgctctcc gtctcctccg cgtgtgcccc cggctcgccg gagagcgtcg atctggagct
     1501 gagaccatgc acgatcgggg gggtcgaggc cgagtgcggg cggctgagcg tgcccgagaa
     1561 cccacacgca ccggacggcc cccggatccg gatctccgtg gcgctcgtcc gggcccgcga
     1621 gtccgatccg gcgccggacc ccctcttcta tctcgctggc tggggctcgg cggggatcga
     1681 ggacgacatg ggggccgcgg cgccgatgct gggagagatc aacgagcagc gcgatctggt
     1741 gttcatcgac cagcggggca cgggcgggtc gaaccggctg gactgctccg tgccgacgga
     1801 gcgcgagatc gagcggacgg ggctccgggc ctacaccgag gccgcccgca cgtgtgcgga
     1861 ggagatcggc ccgaacctcc gctactacac gagttcggtg gcggtggacg atgtcgatcg
     1921 ggtgcgcgag gcgctcgggt acgagcggat caacatctac gggggctcct acggcgtgac
     1981 cctgggtctg atctaccttc agcggcacgg atcgcacgtc cgatcggccg tgttcgacag
     2041 cggctccctg ctcgacgtcc acatcttcga acgggcagct gtgggcggtc agcgagccct
     2101 cgagatgctc ttcgatcggt gcgaggccga cccgagatgc gggatggtgt tcccggacct
     2161 tcgagacgag tgggagcgtc tcgccgcgcg ggtggagtcg aggtcgatcg ccgtccccgg
     2221 gcggcaggaa cgcctggatc cgatcatgct cgccggcctc gtggaggagt tgctgagccg
     2281 gacgcccacc aagacgatcc ttcctcgcgt gatccacctc ctggcaacgg gtgagatcga
     2341 gaccgtggcg gagatgttcc cccccacgtc tccccccaca gaggagccgg cctacatggc
     2401 gctcatccag tgcagcgaac cgtgggcctc gcggcgtccc ccacacgtcg cacggctcag
     2461 cgaagggacg ttcatggagc cgctgctccg gcgggccgcg gcgatcacgc aggcgacctg
     2521 tcgggcgctt cccaggggcg aggcgcccgc cgagatcggg cgccgggtcc gatccgacgt
     2581 gccggtgctg ttcctcaccg gcgacgagga tcccgcggac ccgccggcca acgtcgcccg
     2641 cgctcggagg gagctgccga acagcctcac cgtggtgttc ccggaagccg gtcacggcca
     2701 gctggaactc ccgtgtgccc aggatctcgt gtcggagttc gttcgtgccg gttcctcgga
     2761 ggggctgaac acctcctgtg cggcaaccgc ggcgctcctt cccttcgaca ctcgaacgta
     2821 agcggcgcga cgatctccag gcgggcgcct cgcgctcgtc gaacggggct gaacgtcgct
     2881 ctgcgggggc ccgttcgcat cttcaacatt cgcgggccgg gaagagaggg acttgcccgc
     2941 tggtctggct gaggggggta cgagtcatcc cccgtaagag acccagcccg attcgcagga
     3001 gggtgggtct cggatgctcg aaagaatggc acgggagccc tcgacctcga tgacggaagc
     3061 cctggcgagc gggggccatt gggccctttc tgattcgatt cgcttcgcgg acgttgggca
     3121 acgagacaag cgtcgcaggg aagggacgat ggcgaatgcc gcgttccgcg gatacggctg
     3181 gtgatcgggc gatgcctgcg cagcacgacc tgcgcggcct gctcagggag gtccacgacc
     3241 gagctcggct gaactgggac gcgctgtcga cgatggggcg cctttccatc tcgggagtgt
     3301 gcgtgtcctc gctgttggtc atcggactcg gggtcctgct cccgcgagtc ttcctcggac
     3361 acgccctggg ggcccgcgtc gacgcggtgc actcgatggt ccatgcgctc gagagtcagg
     3421 gcttggtgcc cgctcccgat gtcccgctca ccggtgaggc gtacgagcgc ttcgaccagg
     3481 tcgtgcgggg aggtctgctc ggtggagaga acgcccacgt gaagctgtgg accccctcgg
     3541 gtgaggtcgt ctactcggac accgggagcg aggtcggtcg cgagcatgcc acgagcaggg
     3601 ggatgcagga cgccctcgaa ggccagccgt ttgcccagat cagagatctg tcgactcggg
     3661 aacccggccc cgagcacgac cgtccctcgc gagccgtcgt gctgtatgtc cccgtccatc
     3721 aagagggttc ccggccgatc ggggtcttcg aggtgcacca ggacttcgag gcgctctccc
     3781 gacgattggc gttggtacgg ggggcggtat gggccgcgat cgggttcggt ctcttcgtgc
     3841 tcctggccct cctcgcatcg ctgttcgcgg ggacggccag agtgatggag cgggagaaac
     3901 gagatgcgca ggagcgggcg gaggatctct cgctcctgct gggaacctct cgatcgctct
     3961 cctccgagcc tgccctcacc gcgagcgcat cccaggtcct gacgacgctg accgagcgcc
     4021 tggggcttcg ctgtgcagcc atcctcacgg acgaggaagg gtcaggctcg gcggtgacga
     4081 cggcgggccg agacgcgctc tgttccgcag ctctagagct tgcccggcgc acgtgcgaag
     4141 gcggctcggg gaacgaccgc ctcgtcaggg gggatcccgg cctgggccac ggggcgccgg
     4201 atcatcgctg cgccgtgctc ggcatcccct tccaggcgag gcaggatgcg tgcggggcga
     4261 tcgtggtgtg ccgcgacgcc gatgatccgt tcgcggatcg ggagcgcacg ctgatctcgg
     4321 ccgtggcggg gcagctcgga ttcgccgccc gcaacgatcg actcctccgg ggcttgcgcg
     4381 agatgagcgc cgtccgaggc cgcctcctcc gccgactggt gcacgctcag gaggaggagc
     4441 gtcgccacct gatcggcgac cttcacgatg ggctcggaca gtccctgacg agggtgctgc
     4501 aagggcttcg cgggtcccgg gctcggcttc ctcgaacgga gccggcgatc caggccgagc
     4561 tggaacggct ggagtccctg gtggacgaac agtcgagagg actgcgcggg tatctccgaa
     4621 cgatccggcc cgcggtcctc gaggacttcg ggctcgcggc cgctctcgag gccttcgcga
     4681 gggagcagga gtccgagagc gggatccgga tcgccttctc cctcgaccac ctctcggatc
     4741 tcgacccccc gacagagctc gtcctgtacc gagcggcaca ggaagcgatc atgaacgccc
     4801 ggaagcactc gggggctcgc cgcctgtggc tccggctcaa gccgagcgag actgatgcgg
     4861 tgctcgaggt ggaggacgac ggtcgaggag cgaccgagat ccgagacggc atgggtctgg
     4921 cctcgatgcg tgacagggtc gcctccctcg gcggcacggt cgaggtgcac agcagcccgg
     4981 ggcgagggac ccgcgtgcgg gtgctgattc cgatggaggc cgagcatgga gcccatcagg
     5041 acgttcgtcg ttgacgacca cgagatcgcg cgcgaggggc tcaggtggat gatcggcgcg
     5101 gacgcgacca tccaggtcgt cggagaggcg gagaccgcga gcgaggccat gcaggccatc
     5161 gtgcgctctc tccccgatgt ggtcctgctg gacatccatc ttcccgaccg tagcggcctg
     5221 gaggtccttc gtgagatgca ggagcggttc cccgacctcc cggtggtcat cctgagcatg
     5281 gaggacgatc ccgagtacgt ggagcaggcc gtccgcgccg gcgccgccgg gtacctcgtg
     5341 aagaacgcgt cgcgcgacga gatggtgcgg gctgttcgag ccgcccacgc gggagggggc
     5401 tacatccagt cggaggtgac ccggcccctc ctggctcggt tcgcccggga ggtgcgagcg
     5461 cacggcgatg cgccccattt gtccccccgg gagctggagg ccgtctccct cctggccgaa
     5521 gggctcacca acaggcagat cgccgaccgg ctgggcatct ccgagagcac ggccaaaggc
     5581 tacctgcgcc aggtgtacga gcggctcgga gcagccgaca gggcccacgc ggtagccatc
     5641 gccctccgga gccggatcat cgactgaccg gctgggcgat cgccgtcacg ctcagcgcat
     5701 gaaccccctc ctatcggagg gtgaggctca cggcatcgcg gagtccatcg accctccccc
     5761 ggggatggct gttgaggcca tcctgaggcc atcgaggaag ggaggatgac atgacctacc
     5821 ggacgttcaa gtggatctac gggatcctcg cctccgctct cgcactgagc ttcttcgggc
     5881 acggagcatg ggccgtgttc gagaacaagg agtcgttccg cgagctcctg agcggcacca
     5941 tgaacaacgt gttcggggcg tccacctcga tcgatgacgg ggcgatctcc gctgctgtcc
     6001 gggcgatcgg atggtgggac atcgtgttca cggtgggcat ccttgctctg gcctacgggg
     6061 tgatcaaggg gcacggtgcg ctcttccgcg tggcgagctc ccccgtggcg ctcttcgcct
     6121 actcctgggc gcttctctgg ggcttcctca cggcagggtc acgcgtcacg gccgccggcg
     6181 tcttctaccc ggaggtctgg gacgtcgtgg agcgcggacc caacttcatc gtgcccgtgg
     6241 cgatgctgta catgacgtac ctgctccgac gcccctcgca gctgatcgag cgcgaggagg
     6301 tacccgcaga ggagaagcta caggaagcgc caaccgtgta gagatgttcg gttcgtaccg
     6361 cgaagcggcc tcccgggtta cccgggaggc cgcttcgcgg taagggttgg cgcggaccct
     6421 cccatcgtga tccgtccgaa cggagggggg agggcccgaa ccgttcggtc gctcaggccc
     6481 ttccgctcgg ctcaggagag cacgaggatc gatggcaagg cgagaggcct cgtgaggagg
     6541 cggatcgaat gccaaggcga ccacccggtt gccgaggggg gaggtgacgc gcgatgtggc
     6601 ggaagaacgt gggcccggtc gaccgcgcca tgcgtctgac gggaggggcc ctggtcctga
     6661 tcctcggagt ggttgtcctc gatggactcc agggtcgggt ggttggactg gtggtatcgg
     6721 tcctcggcgt ctggttcatg ttcaccggcg ctgtcggaag gtgccccctg tacgcgctgc
     6781 ttggtatcag caccctgcgt ggagagcgga cggcggacga caccatccga gcagaggact
     6841 tgatgtccac gcgcggaggg ggccgcactc gggcggctta agggtggagg cgacgtgagg
     6901 aagctcgggg gtcgcggcgg ggcactcgtc ttcatcgccg ccgcgcgccg ccgctccggg
     6961 aggagctgat cgtgctgatg atgggggaga cgactgggct ggtcggatcg cgggtgcccc
     7021 gatgatgctg ctgttcgggg gcggtctggt ggccttggca gtcttcctcg gatgaggctt
     7081 cggtgctcga cccctcacag gggcaggacc aagcgtagga aacgacctgg tgccggcccc
     7141 atccaggaac gaggatcggt gatgaagaag gggggagcgt gatggaattg agcgagtggc
     7201 tgtcgttcat ccatgtcctc acggcgatgc tctgggtcgg aaccggcgtc gccctcaacg
     7261 tggcaacgtc gcgagtggtg gcgagcggag acgacgagcg cgtcgtccag ttcacgggcg
     7321 atgtggagtt cctcggcaac tggctgatcg gtccctccgc ggcggcggtg gtaggcaccg
     7381 gaatctggat cacagccatc gaggactggg ttgcgttctc gcagctgtgg atcatcctgg
     7441 cgctcgtggg agtggccgtg tcgatgctgc tcgggatggg ctacttcgga ccggagggca
     7501 ggcggctcgg ccgcctggcg ggggagcgag gagcatccga tccggacttg aagacgagga
     7561 cccgccgcct gcttctcgtc tctcggctcg acatcctcgt cctcgtgttc ctgacctggg
     7621 acatggtgtt caagcccgga ctcgagtgac cggcagcacc cgcagtctcg aaggcgggat
     7681 cgggctcgtt agcgctggtc cgtgcgtcga tcgagaaccc ccggccgaag cgaatacatc
     7741 agatgcggga gcccgcccca catcgccttc cgctcgatcg tcatgcccag cttctccgcg
     7801 acacgccgcg acgccgcgtt cgcctcgctg atcatggaga cgattcgatc gatcccgagg
     7861 ttgtcgaagg catggtcccg ccacgccgcg cccgcttcgg tggcgatccc cttcccccag
     7921 tggctccggc gcacgatcca gccaagttcg gtctcctggc tcccctccac cagggtacgg
     7981 atcaggccgc agtccccgat cagttccccg gtggcccgga ggacgaccgc gcaccggccg
     8041 actccgtcgg tctcatacct gtggaggttg cgctggatcc agtcacgcgc tccctcgcgg
     8101 tcgagagggc tcccccagaa cgtcagcgcc tcagcgtccc cgaggagcga cgccatgtcg
     8161 tccaggtcgc ggagggtcag agggcgcagc accagtcgtt cggtctcgag gcgaagatgc
     8221 gtcatgctcg gactccgcag aagaagggta cccgtacgag gagcctcagc caccatgcat
     8281 cgacgacgaa gtgcgctcga gcgcttgcct cctctgctcg aactctcatc acctccctcc
     8341 gagtcagcga cgctacttca gatgctcagt caatctgttc ggggccgagt ccgcgtaggg
     8401 ccaagagtcc cgtggtcggt tctcggaatc ccgtccgatt ggtcggggct cctcgtccac
     8461 tgcgagcgcg aggagacgag gggaactggg ggtgacggga tcagagagcc tcgtgccggg
     8521 tgacccgcac ccggcgccac cacagggcgg cggagatcac gagcagcacg accgtcgagg
     8581 ccagcgcgtg gagagtgggg agggcaaggc cctcggccgc accgcgcgcc acctccacca
     8641 gcttgagagg gccccacagg gggaggaacg gcgccagtcc ggaatcgagc ggcaacccca
     8701 tctcgatccc gacgacggcg atgagcgtga gcgcgccctc cagctcccga gggaacaccg
     8761 cggccacggc gagccccaga gggacggcga ccagtccgct cagagcgacg gcaaggacga
     8821 gcgcccccgg ttcgggcggc gaggagaccc ctaccatcag ggcgctgaac aggacgacga
     8881 ggacgagggc gaggccctcc aggagcagca gccgtccgac caggacctcg cgcggacggt
     8941 agcccgcgag gaccagccgg gagtcgatgc gacgcgaggc gagggtgagg aagagcgccc
     9001 ctccggctac cgcccaggcc atcccggtcc cgccggccac gagggcgaac tcgtcctcgc
     9061 tcggcgttga caggtagaag gccagcggca gcagcacgag gagcgccatg gccgcccttc
     9121 tgcgagcgag gtcgcgagct tgcgcctcgg ccaccgcgaa gatccgcccc accatcagac
     9181 cggccctccg gcgggctccg gctcgcggcc tccggtcgcg tgcaccggaa gctccacgac
     9241 gcggtccacg cgcgtgagct cggccatcat gtgggtgacc acgaggaccg ccttcccggc
     9301 ggatctccaa tcatcgacat ggtcccagaa gttgacgtac gttccccggt cgaagccctg
     9361 gtagggctca tcgaggagca ggacctccgg gtcgccgagc aacgccaggg ccaggttgag
     9421 cttctgacgg gttccaccgg acaggtcctt cgcgaccgcg cgctctcgcg ccgggaagcc
     9481 gaaccacgcg aggctgcgcc tgccctcccg cagggccgtc tgcctggaca ggccggccgc
     9541 gcggccgaag agcaccatgt gctcgtccgc cgtgaggagc tccaggacgc cggcgtcctg
     9601 ggggcagtag ccgatccttc ccggcacgtg aacggtgccg gagtccgcgc ggaggagccc
     9661 ggcacagatg cggagaaggg tggtcttccc cgcgccgttc tcccccacga gggccacggc
     9721 ctcgccgggc cggaggtcga ggttcacgtc ctccaggacg gccttacgcc cgaatcgctt
     9781 gctgatgccc ttcgcgacca gcgcaacggg tcgatcacct cgaaggctcc tggatgccac
     9841 tctcgactcc cccgcttgac tccgcagtct cgccatgcgc cgcgccgatg taccagggcc
     9901 gtccgtccct gtcgaagcgg gacctgcgac tctccctccg gtgaagacgc tcgctgagtg
     9961 gatggcacct caggactcgg ggccggacgg cccctgacgg tcgggtcgtc cgcatcttcc
    10021 acctgtcgcg acgccgggtg gagactggga tggctcagga aggatccgat gctccagatc
    10081 gacgacttgc gcaagcgcta cggagatctg gtggcgctcg acggcgtctc cttcgaggta
    10141 gcagccggtc ggatcgtcgg attcctcgga cccaacgggg cggggaagac gacgacgatg
    10201 cgggccatct tcggcttggt gcgtcccgac gggggcgagg tcaggtggaa ggggaccgcg
    10261 gtcggccccg tggagcgagc ccgattcggc tacatgcccg aggagcgggg actctatccc
    10321 aagatgaggg tgggaacagc tcatctactt cgggcggctc tcgggcatga gctctgcgtc
    10381 ggccacggat gcagcagaga catggctcgc tcgcttgagt ctcggtgaga gggtgggggc
    10441 gcgactcgag gagctctccc acggcaatca gcagcgggtc cagctcgccg cggcgctcat
    10501 ccacgatccg gagctggccg tgctcgacga gccgttcagc gggctggacc cgctcggcgt
    10561 ggagtctctc ggcgagatgc tcgggcagac ggccgcgctc ggcaccgccg tgctcttctc
    10621 gagccatcag ctcgacctgg tggagcacgt ctgtcaggac gtcgtgatcg tcgatcgcgg
    10681 gagggtcgtc ctggcgggag cggtggacga gctcaaggcc ggatccgcgc gccgctcgct
    10741 ggaggtcagg gtggacggcg agcagtgggt accggtcctc ccgagtggga ccgtgacgag
    10801 caagcaggac gggcggctgc gggttctggt cgacgcggcg gtcgacctgg acgagatcct
    10861 ggccagcgca cggcgggcgg gggaggtcac gacgttcagc ttcgagccgc cctcgctgac
    10921 ggatctgttc ctcgaggccg tctcggaggg gcgaggcaca tgagcggagg atggcgctcc
    10981 ctgtggctgg tggcccgacg ggaatgggac cagcgggtgc gctcgctctc gttccgggtc
    11041 tcgacgttgc tctccatcct catcgtggtc gcgctcatcg ccgtgcccga gatgtacggg
    11101 ggaggtgcgg aacccaccca caccgtcggc ctcgtcggcg acagcggagc ggagctgccc
    11161 gcgagccttc gctccgcggg cgaggagatg gggctctcgg tgaaagtccg cgagttcgcc
    11221 gacgagtcct cgggccgggc cgcgctgcga tccgaggaca tctcggttct gctggtcgat
    11281 cagcgggacc tggtctggag ggcggaggtc gatcaggggc tccgggcggc ggtgacctcg
    11341 gcggtgcagg ctctcgatcg tcgggaggcc atcgcggagc tcggcctcac ctcagaggag
    11401 gccgggcggc tgatcggtcc cctggggctt cgttccacga gcctcgagcc tgcggcggcc
    11461 gagcgctcgg ctcgtcgcga cctcggcttc gtcgccgtgg tgatgctgtt catggccatc
    11521 gccttctacg gtggcttcgt tctggtcggg gtggtggagg agaagtccag ccgggtggtc
    11581 gaggtgctgc tcgcgcggat ccggcccgcc cagcttctcg gggggaagat cctcggcatc
    11641 gggctcgtcg gtctggcaca gttcgcgctc gtcgtgggct cagcgctggt cgccgtctcg
    11701 ctctctggga acaccctgct gcccagcacc accccgagca cgctcgggtc gatcctgttc
    11761 tggttcgtga cgggctacgc cttctactcg gtgctgttcg ccacggccgg gtcgctcatc
    11821 tcgcggcagg aggaggctca gagcgtccag ttcccggtca cggcgttcct cctggtggga
    11881 tacgttgtgg cgttccaggc cgccgcggcg ccggaaggcg tggcggcgct cgtcgggtcg
    11941 ctcctgcctc cgacggctcc gatggtcatg accgtcctga tggcgcaggg aggggtcgcg
    12001 tggtggcacg tcgcgctgtc ggcggccctc ctcatggcca cgacctacgg catggtgctg
    12061 ttggccggac gcctctactc gggcgcggtg ctcaggctcg gccgccgagt gcgcttgcgg
    12121 gaggcctggc acggcgcgga gggctgaccc cggtcacgcc gcgggaaccc ccggccttcg
    12181 ctctcggctg catcccgatc gagagccgct caggttcgga ccgtgaacgc gctggtgccg
    12241 gccccgatct cgatgtcgta ccgcgcctcg gccccctcgt agtcggggct ctgccagtcg
    12301 gtcttccccg cggacgcgaa gcggaggtcg tcgatggtca ggttcgacga cccgcccccc
    12361 gtgcggaccc tgatgggaac cccctccggc cggatcaccg tgatgtcgct ggcgccaccg
    12421 ccgatccgga tcgggacggc gaccttcggg
//
LOCUS       DASUTZ010000273        18924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_887844, whole genome shotgun sequence.
ACCESSION   DASUTZ010000273 DASUTZ010000000
VERSION     DASUTZ010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 18924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18924
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887844"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VF097_11840"
     CDS             complement(<1..348)
                     /locus_tag="VF097_11840"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX6263526.1"
                     /translation="MTGLLAQAHRPPEPFEAPGVEILDPPAIWEFTLFGIHFDITRVT
                     LYLFLAVALVVILFMLAARRPQLVPRGVQNFVESIVQFIREEIALQVIGREGLTWVPF
                     LTALFVFILIGNIF"
     gene            complement(345..809)
                     /locus_tag="VF097_11845"
     CDS             complement(345..809)
                     /locus_tag="VF097_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263527.1"
                     /translation="MEEKIARPEHAMVLKALAAGAAAAPVAFGAGFVAGGGAAALSAL
                     LGVVLVVLNFAAHGLSLAWAAGVSIPAVQAVALGGFVGRMGLIAGTMVALDRAAFFSP
                     LVFGLTVIAATLALLVYEARLVRAGLGGALEIPPDPVAVAAREALRLKESTP"
     gene            complement(784..996)
                     /locus_tag="VF097_11850"
     CDS             complement(784..996)
                     /locus_tag="VF097_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263528.1"
                     /translation="MADNPWSGFGEAWAVTATLIAGMVAWGGIGYLLDLWLGFRWLFL
                     PIGAVVGMGGGIYLVYIRYGREDRET"
     gene            1273..2370
                     /locus_tag="VF097_11855"
     CDS             1273..2370
                     /locus_tag="VF097_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6263529.1"
                     /translation="MTELDVRLVDVVKSFGDVVAVDHVDLEIRRGEFFSLLGPSGCGK
                     TTTLRMLGGFEQPDGGRIELRGSDVSGVPPHKRDVNTVFQSYALFPHLDVFENVAYGL
                     RRKKTPKDEVRRRVAEMLELVDLPGFEPRRPGQLSGGQAQRVALARALVNHPQVLLLD
                     EPLGALDLKLRKQMQLELKRIQHEVGITFIYVTHDQDEAMTMSDRIAVMRSGRIEQVG
                     APEEVYEEPATEFVAGFLGASNLLTGTIEGRDGAHAVVVLASGEKVRVPHDRVRADLR
                     EAHLGVRPEKIRIAPGESAPPEGRNAIRGRLLVSTYVGVSSVYTVEAPGGEQLTVYAQ
                     NVDAPEERAPATGEEVVLSWHPQHTFVVEPG"
     gene            2382..3626
                     /locus_tag="VF097_11860"
     CDS             2382..3626
                     /locus_tag="VF097_11860"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX6263530.1"
                     /translation="MTEGMNELFGGLRDPMSRRELLRRAGRGSVFLGVGALLAACGIQ
                     GEDEEAGPTPTEASPSPLPEIAGQVVMANWPLYIDRAKGESPTLVSFTEETGIQIDYQ
                     VVINDNEEFFGTIQEPLSRGQSAGYDIIVVTDWMVAKMIRLGFLQPLHHDRLPNFEAN
                     AFEKFKDPSYDPENTYSIPWAAGITGIGYDVERTGRELTSMEDLWDPAFEGHVGMFTE
                     MRDSFGFALLANGVVPEEATMEDVEAAQQKLLEQREAGIVRGYFGNDYTDQLATGNLW
                     VSMAWSGDVFALQQDNPNLRFIIPEEGAMRWTDNMLIPVGAEHPADAHEFMNFVYDPR
                     IATNITEWVWYESPVSQVPDLIRQDAAEASGGEAELLEQIAESPLVFPSEEVEAQLYN
                     YKNLDEEEEAAWNELFQAVVQG"
     gene            3631..4515
                     /locus_tag="VF097_11865"
     CDS             3631..4515
                     /locus_tag="VF097_11865"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008745372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6263531.1"
                     /translation="MATTAETAAPRRARWRRGSAPYSLLLPGGLWLLVFFVVPMVSML
                     SVSLQEGSLERGYELTWRFQTYLDVLGKFDTQFIRSIFYGLIVTAALLVICYPLAYTI
                     AFRGGRWKNALLFLLIMPFFVSFIIRTLEWKFILADDGFLFGTLKNLGLVGEDFRVLA
                     TPFAVIGGLVYNFLPFMALPLYVALERMDRSLLDAAGDLYSSRASAFLRVTLPLSLPG
                     VFAGSLLTFIPAVGDFINAEVLGGINTTMIGNVIQQEFLVNNDYPEAAAISFLLLAGI
                     LIGVAVYAKLLGTEELTG"
     gene            4512..5384
                     /locus_tag="VF097_11870"
     CDS             4512..5384
                     /locus_tag="VF097_11870"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6263532.1"
                     /translation="MSVRPPPRDVVAPPGATETPVRRPEGRGWILPAYTGLVIVYLVL
                     PILVMIVFGFNDITGRFNFTWQGFTLEHYRTLGDFPELIEAIKNSLLIAAGATIVSTV
                     LGTMMALALTRYRFRGKAGANLFIFLPLGTPEVVLGASLLSLFITLGMDRGLLTILIA
                     HIMFNISFVVVTVKARTQGFNREWEEAAKDLGAAPWTAFRTVTLPLIFPGVLAAAVLA
                     FALSVDDFVITLFNAGGELTFPLWVYGASRFGVPPQVNVMGTLIFVIGVALALGSVWL
                     QRRRGEEIVAGYLR"
     gene            5394..6749
                     /locus_tag="VF097_11875"
     CDS             5394..6749
                     /locus_tag="VF097_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX6263533.1"
                     /translation="MDDRITARAREIAKEETARLLERTPASAKLYERASRSMPMGVAS
                     SFHAAEPYPVYLEGGRGSRVWDVDETEYIDFHNGFGTMAVGHAHPKVVEAIRRTAGEG
                     THFAVTTETAVAFAEELRRRFRLDMVRFVNSGTEATMDAIRIARAATGRDTVLKIAGS
                     YHGHHDTVMFSVVSNADMMGGRDVPPDRPMSKGIPPEMAKRTLVVPFNDADALEAMLD
                     ERAPDVACLIMEPVMMNIGIVLPQPGYLERVRELCTRHGVVLIFDEVKSGATIAEGGA
                     TERFGVQPDLACFAKAIGGGVPTGAFGGKADVMDVINHGAAQQGTFNGNPLAAAAGLA
                     ALTEVLTPEAYEHMGAIGTRLAEGCRAAIEEHDIPAHTVDLGCKGCVSYRREPLRDYR
                     DFLQANGELFEASWAWAMNRGIFMTPGDEEQWTLSVQHSEEDVDRFVEVFAEFCAAVA
                     S"
     gene            6746..7567
                     /locus_tag="VF097_11880"
     CDS             6746..7567
                     /locus_tag="VF097_11880"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6263534.1"
                     /translation="MSDPVLLEEQLGPVRRLTLNRPGALNALSSKLIEELSAALGRAA
                     EDDDARVVVIRGAGRAFCAGYDLKEEGRSGEMDAVAWRNELAHDLRRMLEIFDHPQPV
                     IAEVHGYCLAGGCDLMMMCDVAVASDDARFGEPEIRYGSGVVTLVMPWLVGARKAKEL
                     LLTGEDRVPAEEALRIGLVNRVVPADRLEEETLELAREIAVMDPVSISLTKAAINRSL
                     EASGFREALWANLDLDALIEAAEVPERKEFNRIREEEGLKAAIRWRDERFRMEEG"
     gene            7564..9660
                     /locus_tag="VF097_11885"
     CDS             7564..9660
                     /locus_tag="VF097_11885"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX6263535.1"
                     /translation="MTSVRGLRPLLAPRSIAIVGASEAEGSWAPEFYENLRAAGFEGP
                     IFPVNPKYREVWGFRCVPSAADLPEGVDLAVIVVPARHAVSAVEDAARAGVRSAMVVS
                     SGFAESGGEGAAMQEELAAAARRAGMPVIGPNVEGYVNVTDRVWCYGTGRPPPLAPGG
                     LTVVSQSGTVAWSLAQMAGDRGLGVRLVIGVGNEASVGLADMLTWAAADPETTVVACY
                     VETVRDPAGFAGAADALREAGKPLLVCAPGHGEIAGRAVIAHTGAVAGRTGVRDAWLR
                     RLGAALVPDSVALFEAAALASRATRPKRRGVAVAFQSGGSCTLFAEHAEAAGLEMPAP
                     SAATVRRMRRALPPFAKPGNPLDVTGQASVETPMFEGALEALAHDPAIGVVAFDAFPS
                     RDPVQGSWEPSVLAAAERLARETGTVMISLAPVPLALDDAGRKSAEHLRIPVLQGIQA
                     APTAIRALLDLSQTSGGRTQAPHPARRRAGALLRGRSGPLDELRARNLLGLYGIEAPE
                     EAVAHTPDEAARAAARLGFPVAVKALAAALPHKTAAGAVKVGLRAGPEVERAAAAVLR
                     AARRAGVAPDGILVQRMASGAEVLVGGVWDERFGPAVTVKPGGAAAEAGEAEFLAAPL
                     RAGEAAAFVGRRAARLGLERRRHDLQAVAAALDAVARLLVDLKGRLVELETNPLIVSR
                     KGAVAVDALAVLSERA"
     gene            complement(9644..10108)
                     /locus_tag="VF097_11890"
     CDS             complement(9644..10108)
                     /locus_tag="VF097_11890"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HEX6263536.1"
                     /translation="MFEKISDPKEVRLVEGYIPLEHRYTPGVAGEAFFASLMKGEFLA
                     SGCERCGVVYCPNRIFCERCMGELDADRTVGPRGRIESFTVVYAGVEGEPLEEPRTIG
                     LVRLEGADTVLMHELVGFDEGPQIGDEVEPDFEPRNRRTGSILDLRGFRPAR"
     gene            complement(10109..10750)
                     /locus_tag="VF097_11895"
     CDS             complement(10109..10750)
                     /locus_tag="VF097_11895"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HEX6263537.1"
                     /translation="MTPKRDLRAVDETQPTTFYGPTYLKSEEFRKAIGAVDFAVDARY
                     AWDTGVAISRFLEGLKEGRILGRECRSCGRTLVPPRMFCEECFRPTDRWVEVPDRGTV
                     NTFSICYIQWDMVELEEPELPFVLELDVDTPMMGFMHKLGEVDPERIEVGMEVEAVWK
                     PEGEREGSILDIRYFRPRDAAMRKGTRRKTSARKSPAKKTRSRKRSGARRKAG"
     gene            complement(10747..11910)
                     /locus_tag="VF097_11900"
     CDS             complement(10747..11910)
                     /locus_tag="VF097_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014512167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HEX6263538.1"
                     /translation="MGQRVAAVGAGMTRFVRRAQETGKELAWEAARAALEAADMTIDD
                     IDCVTMGTAPDAFDGVHMKSEYLADGAGAWGKPYYRTYVGGGTGVFSAIHGWWQVASG
                     QFDTCLVVAEEKMSSTQPHPQGAFITIFDNMIERPLGPNLLWIFALEMNRYMQVYGLD
                     KADIAAVSVKNKRNAADHPAALLGDADITVEDVLNSETLAWPVQRLDVSPVTDGAVAV
                     VMAAEHVAKKKSPMPVWVEGVGWHLDTAYWTNRDLVYPRYVEYAARQAYEMAGIREPR
                     KEIHIAEPYDPFDYKELHHLEGLQLFEKGKAPEAMRDGVLNRDGDLPSCPSGGLLGVG
                     NPIAAAGLMKIAELYWQISGQAGKRQVPGDPRRGVAQAWGDLMQVGTVVVMGR"
     gene            complement(11910..12281)
                     /locus_tag="VF097_11905"
     CDS             complement(11910..12281)
                     /locus_tag="VF097_11905"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6263539.1"
                     /translation="MPRLRRLDHVYYWTQDMDRAVKFYRDVLGLELIRQEGSSWAEFE
                     TGTIRLALHGAVEGRPAEAGGATAVFEVDDLDATRAALEERGVELLDHMGEVEGFARF
                     ASFLDPDGNTVQVIEYTKGAS"
     gene            complement(12281..13447)
                     /locus_tag="VF097_11910"
     CDS             complement(12281..13447)
                     /locus_tag="VF097_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEX6263540.1"
                     /translation="MNDRVAIVGVGHAGFAPITAGVSYKELMFEAAMRAYDDAGVDVR
                     RDVDSFVGCSEDFWEGTSIFDEYLPDQIGGALRSVHTVSADGLFGIATALMLIRSGHA
                     EVVAVESHSKASDILTPGSIHEFALDPVYNRPLRVTPHAIAGLEMHRYLEVSGTTEEQ
                     CALVVAKNRRMALDNPRAAYPTEVTAADALDSEPLAWPLKRLEVADRADGCVVMVLAS
                     EGRARDLTDAPVWVLGTGWSTGAASLESREWDRSLDARQAADRAYRMARIGHPTGVVD
                     LAEVDDRYAYRELMHLEALRLAHPGEAGAMLEDGIFGRDGDVPVNVSGGSLGQGNLFE
                     ANGLARALECVLQLRGEAGERQVEDAGVAVAQSWRGVPTATSAVAVFASDEAVA"
     gene            complement(13496..14143)
                     /locus_tag="VF097_11915"
     CDS             complement(13496..14143)
                     /locus_tag="VF097_11915"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6263541.1"
                     /translation="MTAPDPEFIRRRTTEAVLADGTRVLLRPLVPEDGPKMLEAFDRL
                     SPESRYRRFMSPVDELSPEMVRKLTDLDYVDHFAWAALAVDEPGRPGIGVARYVRAED
                     DPQVAEAAVTVIDEYQGRGLGSLLLQALGASALEHGITRFRGYVLEDNRAMRDILEEL
                     GGTAHHDGAGIVRVEVELPIADELRSSPAYRALRALARGEGPRLLRWGALWEHPG"
     gene            complement(14140..14754)
                     /locus_tag="VF097_11920"
     CDS             complement(14140..14754)
                     /locus_tag="VF097_11920"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX6263542.1"
                     /translation="MAFPSRMSEGEHQVLALRPHWMGVVAPLVITVLLLVGMAAALLS
                     IPESWPSWVRWIVVGAFSLILLVWPFRRIGAWVTSEYVITNERVVHRQGWLTKRSVEI
                     PIMGVADVVFSQSVLQRLVRVGDVVIESAGGLGFTRLAYIPRADDVHRTLYDLIEDAR
                     VVTSNNHGPPGPLDQIERLARLRERGLLTPQEFEVHKQRLLKQL"
     gene            complement(14818..15636)
                     /locus_tag="VF097_11925"
     CDS             complement(14818..15636)
                     /locus_tag="VF097_11925"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX6263543.1"
                     /translation="MGSETLSQDSLRAAVRAAGIEAPPRYHASIGSTNVEAARLAAEG
                     APAWTVVAAGHQTEGKGRLGRRWVEAPGRGLLMSVLLRPRIDPAEAPLISLLAALAMA
                     DACRELTTAEVGCKWPNDLVIGERKVGGVLAEARIEGGEVIHVVVGTGVNARMRAEDF
                     PQELRPTATSLEVEGEAVHPALLVRRYLERLRRGLGREGFSPALLDAYRERCVSLRRT
                     VRATTTEGEEVEGMAVDVDERGGLVIETPRGRRAVAFGEVAHLAPEGLPTRPEA"
     gene            15668..17389
                     /locus_tag="VF097_11930"
     CDS             15668..17389
                     /locus_tag="VF097_11930"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX6263544.1"
                     /translation="MGKPRVLIANRGEIAVRVIRTCRELDIPTVAVYSDADRDALHVE
                     MADRAHHIGPASPASSYLNVGAILEAARRSGATMVHPGYGFLAENFDFARACAEAGLT
                     FIGPPPEAMERMGDKAAARRAAERAGVPVIPGLSEPVDAREAARVAERIGFPLAVKAA
                     FGGGGRGMHLVATPAELEEAVERSSREARAYFGRPEVYLERYLASAHHVEAQILCDEY
                     GNASFLGERDCSLQRRYQKLVEESPSPVVDENLRSRIGEAALAIAKASGYRNAGTVEF
                     LVEDDGSFYFLEMNTRLQVEHPVTEMVTGLDLVRMQIEVALGEKVDPGTPDLRGHAIE
                     CRINAEDPAADFLPRPGFVRRLVFPSGPFVRVDAGITEGRTIPGDYDSLFAKLIVWGE
                     DREAARRRMLRALDELEVEGVPTTVPFHRWVLNTIAFREGSHTTKWVERALEEGMFRS
                     EEAEPASSAPPDPPGHLVVEVGGRRVPVRVWGDDRAAPPPAPAAAAQAHHSGAHGAVV
                     APMQGTILKVLVEEGQEIEAGDVVCILEAMKMENHIAATQDGTISSVAVSVGDVVQGG
                     QAIATIG"
     gene            complement(17393..18082)
                     /gene="npdG"
                     /locus_tag="VF097_11935"
     CDS             complement(17393..18082)
                     /gene="npdG"
                     /locus_tag="VF097_11935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HEX6263545.1"
                     /translation="MSEHVAIVGGTGELGFGIALRLGRRGRRVVIGSRSAEKAADAVA
                     RVAEIIGEDAPVEGAENPEAVADARVVFVTVPYAGQAPTYKALKERWPEGAIVCDTTT
                     PLATAVGAAATQVLRPWQGSAAEQAASYLPGHVRFVSGFHTVSAEVLEDLDHDPDCDV
                     LLCGRDGEAKAAVGELVEEMAMRWVDAGPLTMARVVEPLTAALIRVNKAYGIRNAGFR
                     VTGREAWGRPG"
     gene            complement(18079..>18924)
                     /locus_tag="VF097_11940"
     CDS             complement(18079..>18924)
                     /locus_tag="VF097_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX6263546.1"
                     /translation="DVDDFAPRVSFFFACHMSFFEEVAKFRAARRMWHRIMTERFGAK
                     DPRSAMLRFHTQTGGATLTAQQPENNIVRTALEALAAVFGGTQSLHTNSFDEALSLPS
                     ERAARIALRTQQVIAHEAGVIDTPDPLGGSYFVESLTSEIETRAWEYLEKIDGMGGSV
                     QAIEAGWIQDEIHDAAYRIQRGVESGERIVVGVNRYAEDEEEPVELERLDEEAVARQV
                     ERLVALREERDAPAVEATLSAVEEAARGTDNLLYPMREALKARATLGEVSDRLRGVFG
                     EHRPR"
BASE COUNT         2700 a         6839 c         6681 g         2704 t
ORIGIN      
        1 gaagatgttg ccgatgagga tgaagacgaa gagcgccgtc aggaacggga cccacgtcag
       61 gccctcgcga ccgatcacct gcagcgcgat ctcctcgcgg atgaactgca cgatcgactc
      121 cacgaagttc tgcacgcctc gaggcacgag ctggggccgc ctggcggcca gcatgaagag
      181 gatcacgacg agggccaccg ccaggaacag gtagagcgtg acacgggtga tgtcgaagtg
      241 gatcccgaag agggtgaact cccagatcgc gggggggtcg aggatctcga cgccgggggc
      301 ctcgaacggc tcggggggtc ggtgagcctg cgccagcagc ccggtcacgg agtcgactcc
      361 ttgaggcgca gggcctcgcg ggccgccaca gccaccgggt cgggcgggat ctcgagggca
      421 ccccccagcc cggcgcgcac cagccgggcc tcgtagacga gcagggcgag cgtcgccgcg
      481 atcaccgtca ggccgaacac gagaggcgag aagaacgcgg ctcggtcgag ggcgaccatc
      541 gttccggcga tcagccccat ccgaccgacg aaccctccga gcgccaccgc ctgcactgcg
      601 gggatcgaga cccccgccgc ccaggccagg gagagcccgt gggccgcgaa gttcagcacg
      661 accaggacga ccccgagcag cgcagagagg gcggcggcgc ctcctcccgc cacgaagccg
      721 gcgccgaagg ccacgggcgc agcggccgca ccggcggcca gcgccttcag caccatcgcg
      781 tgctcaggtc tcgcgatctt ctcttccata ccggatgtag acgaggtaga tccctccacc
      841 catgccgacc accgcgccga tcgggaggaa cagccaccgg aaccccagcc acaggtcgag
      901 caggtagccg atcccgcccc aggcgaccat ccccgcgatc agcgtggcgg tgaccgccca
      961 ggcctccccg aatccgctcc aggggttgtc cgccatgggc cgagagagta tatcggtcgc
     1021 gcttcgacag cgtcaaacct cgggaggccc cgtttccgca ggtcagaggg tatatgggcc
     1081 gtccggcacc gaactttgtg aatgctttca cgaagtcgtg cggcccccgg cccggcccgc
     1141 ccggcccagg cagggcctct gcggtacgct tccgcttgcg ccggttcgta caaacggagg
     1201 ccgcccttcc ccacgaccgg tacgggcgcg gcggagggcg cggccgtacg ctgtcccgcg
     1261 agggggtgag gggtgacgga gctcgacgtc cgcctggtgg acgtggtcaa gtccttcggc
     1321 gacgtggtcg cggtcgatca cgtcgacctg gagatccggc ggggcgagtt cttcagcctc
     1381 ctcggcccgt ccggctgcgg caagacgacc accctgcgga tgctcggcgg gttcgagcag
     1441 cccgacggcg ggcgcatcga gctgcgaggc agcgacgtct ccggcgtccc accccacaag
     1501 cgggacgtga acacggtctt ccagtcctac gcgctcttcc cccatctcga tgtcttcgag
     1561 aacgtggcct acgggctccg ccggaagaag accccgaagg acgaagtgag gcgccgggtg
     1621 gccgagatgc tcgagctcgt ggacctcccc ggcttcgagc cgcgccggcc gggacagctc
     1681 tccgggggcc aggcccagcg ggtggcgctg gcccgggccc tcgtgaacca tccccaggtg
     1741 ctcttactgg acgagcctct gggggccctc gacctgaagc tgcgcaagca gatgcagctc
     1801 gagctgaagc ggatccagca cgaggtcggg atcacgttca tctacgtcac gcacgaccag
     1861 gacgaggcga tgacgatgtc ggaccgcatc gccgtgatgc ggtcggggcg gatcgaacag
     1921 gtcggcgccc ccgaggaggt ctacgaggag ccggccaccg agttcgtggc cgggttcctc
     1981 ggggcctcga acctgctcac ggggacgatc gagggcaggg acggcgcgca cgcggtcgtg
     2041 gtcttggcct cgggggagaa ggtgcgcgtg ccccacgacc gcgtgcgcgc cgacctccgc
     2101 gaggcccacc tcggcgtccg tcccgagaag atccgcatcg cccccggcga gtcggccccg
     2161 cccgagggcc gcaacgcgat ccgggggcga ctgctcgtct cgacgtacgt gggcgtcagc
     2221 agcgtctaca cggtggaggc ccccggcggt gagcagctga ccgtgtacgc gcagaacgtc
     2281 gacgcgcccg aggagcgggc gcccgccacg ggggaggagg tcgtcctctc gtggcatccg
     2341 cagcacacgt tcgtggtgga gccaggctga aggaggtcgg catgactgag gggatgaacg
     2401 agctgttcgg cggtcttcgg gacccgatgt cgcgacggga gctcctgcgt cgcgcgggcc
     2461 gcggatcggt gttcctgggc gtgggcgcgc tcctggcggc ctgcggcatc cagggcgagg
     2521 acgaagaggc gggccccacc ccgaccgagg ccagcccctc gccgctgccc gagatcgcgg
     2581 gccaggtcgt gatggccaac tggccgctgt acatcgaccg ggccaagggc gagagcccga
     2641 cgctcgtctc cttcaccgag gagactggca tccagatcga ctaccaggtg gtcatcaacg
     2701 acaacgagga gttcttcggg acgatccagg agccgctctc gcgcggccag tcggccggct
     2761 acgacatcat cgtggtcacc gactggatgg tggccaagat gatccgactg gggttcctcc
     2821 agccgctgca ccacgaccgc ctcccgaact tcgaggccaa cgccttcgag aagttcaagg
     2881 acccctccta cgacccggag aacacctaca gcatcccgtg ggcggccggg atcacgggga
     2941 tcggctacga cgtcgagcgg accggcaggg agctgacctc gatggaggac ctgtgggacc
     3001 cggcgttcga ggggcacgtc gggatgttca ccgagatgcg ggactccttc gggttcgccc
     3061 tcctggcgaa cggcgtcgtc cctgaggagg ccacgatgga ggacgtggag gccgcgcagc
     3121 agaagctgct cgagcagcgt gaggccggca tcgtccgcgg ctacttcggc aacgactaca
     3181 cggaccagct cgccaccggg aacctctggg tctcgatggc gtggtcgggg gacgtcttcg
     3241 ccctccagca ggacaacccg aacctgcgct tcatcatccc ggaggagggg gcgatgcgct
     3301 ggaccgacaa catgctgatc ccggtcgggg ccgagcaccc ggccgacgcg cacgagttca
     3361 tgaacttcgt gtacgacccg cggattgcca cgaacatcac agagtgggtc tggtacgagt
     3421 cgccggtgag ccaggtgccc gacctcatcc gccaggacgc ggcggaggcc tcgggaggcg
     3481 aggccgagct gctcgagcag atcgccgaga gcccgctcgt cttcccctcg gaggaggtcg
     3541 aggcccagct ctacaactac aagaacctcg acgaggaaga ggaagccgcc tggaacgagc
     3601 tcttccaggc cgtcgtgcag ggctgagccg gtggcgacga cggccgagac cgcggcgccg
     3661 cgacgggcga ggtggcgccg cgggagcgcc ccctactctc tcctgctccc cggcgggctg
     3721 tggctcctcg tcttcttcgt ggtgccgatg gtctcgatgc tgtccgtctc tttgcaggag
     3781 ggctcgctgg agcggggcta cgagctgacc tggcggttcc agacctacct ggacgtgctc
     3841 gggaagttcg acacgcagtt catccgctcg atcttctacg gactcatcgt caccgcggcc
     3901 ctgctcgtga tctgctaccc gctcgcctac acgatcgcgt tccggggcgg ccgatggaag
     3961 aacgccctgc tgttcctcct gatcatgccg ttcttcgtct cgttcatcat ccggacgctg
     4021 gagtggaagt tcatcctggc cgacgacggg tttctcttcg ggacgctgaa gaacctgggt
     4081 ctggtgggag aggacttccg cgtcctggcg acgccgttcg ccgtgatcgg cggcctcgtc
     4141 tacaacttcc tgcccttcat ggcgctcccc ctctacgtgg cgctggagcg gatggaccgg
     4201 agcctgctgg acgcggccgg ggacctgtac tcgagccgcg cgtcggcgtt cctccgggtc
     4261 acgctgcccc tgtcactgcc gggcgtgttc gcaggctccc tcctgacgtt catccccgcg
     4321 gtcggcgact tcatcaatgc ggaggtcctg ggcggcatca acaccacgat gatcggcaac
     4381 gtgatccagc aggagttcct ggtgaacaac gactaccccg aggcggcggc gatctccttc
     4441 ctcctgctcg ccggcatcct gatcggcgtg gcggtgtacg ccaagcttct gggcaccgag
     4501 gagctgacgg gatgagcgtg agaccgcctc cccgggacgt tgtggcgccg ccgggggcca
     4561 ccgagacacc ggtccggagg ccggagggcc ggggctggat cctgcccgcg tacaccggcc
     4621 tggtgatcgt ctacctcgtc ctgccgatcc tcgtgatgat cgtgttcggc ttcaacgaca
     4681 tcacggggcg cttcaacttc acgtggcagg ggttcacgct cgagcactac cgcactctgg
     4741 gcgacttccc cgagctcatc gaggcgatca agaactcgct gctgatcgcg gcgggcgcca
     4801 cgatcgtctc cacggtgctc ggcacgatga tggccctggc cctgacgcgc taccgcttcc
     4861 gggggaaggc gggggcgaac ctgttcatct tcctgccgct ggggaccccc gaggtcgtgc
     4921 tgggcgcctc gctgctctcg ctcttcatca cgctggggat ggaccggggc ctgctcacga
     4981 tcctcatcgc gcacatcatg ttcaacatct cgttcgtggt cgtgacggtg aaggcgcgga
     5041 cgcagggctt caaccgagag tgggaggagg cggccaagga cctcggggcg gcgccctgga
     5101 cggccttccg cacggtcacg ctccccctga tcttccccgg agtgctggcc gcggcggtgc
     5161 tggcgttcgc actctccgtg gacgacttcg tgatcacgct gttcaacgcg ggcggcgagc
     5221 tcacgttccc gctctgggtg tacggggcca gccgcttcgg cgtgccgccc caggtcaacg
     5281 tgatgggtac cctgatcttc gtgatcggcg tcgcgctcgc gctggggagc gtctggctcc
     5341 agcggcgccg gggcgaggag atcgtcgccg gctatctgcg ataaggagga gcgatggacg
     5401 accggatcac cgcacgggct cgggagatcg ccaaggagga gaccgcgcgc ctcctggagc
     5461 ggacgccggc atccgccaag ctgtacgagc gcgcgtcgag gtcgatgccg atgggcgtgg
     5521 cgtcgtcctt ccacgccgcc gagccgtacc ccgtctacct ggagggaggg aggggcagcc
     5581 gcgtctggga cgtggacgag acggagtaca tcgacttcca caacggcttc gggacgatgg
     5641 ccgtgggtca cgcccatccc aaggtcgtgg aggcgatccg ccggacggcc ggcgagggta
     5701 cgcacttcgc ggtgaccacc gagaccgcgg tcgcgttcgc cgaggagctg cgcaggcgct
     5761 tcaggctcga catggtccgc ttcgtgaact cgggcaccga agccacgatg gacgcgatca
     5821 ggatcgcgcg ggccgcgacg ggccgggaca cggtcctgaa gatcgccggc tcctaccacg
     5881 gccaccacga cacggtgatg ttcagcgtgg tgtcgaacgc cgacatgatg ggcggccgcg
     5941 acgtcccgcc cgaccggccg atgtcgaagg gcatccctcc cgagatggcg aagcgcacgc
     6001 tcgtcgtgcc cttcaacgac gccgacgccc tcgaggccat gctggacgag cgcgcccccg
     6061 acgtcgcctg cctgatcatg gaaccggtga tgatgaacat cggcatcgtc ctcccccagc
     6121 ccggctacct ggagcgggtg cgggagctct gcacccgcca cggcgtggtc ctgatcttcg
     6181 acgaggtgaa gtcgggggcc acgatcgccg agggcggcgc caccgagcgg ttcggcgtcc
     6241 agcccgacct ggcctgcttc gccaaggcga tcggcggcgg cgtcccgacg ggcgccttcg
     6301 gcgggaaggc ggacgtcatg gacgtgatca accacggcgc ggctcagcag ggcacgttca
     6361 acgggaaccc gctggccgcc gcggcggggc tcgcggccct caccgaggtc ctcacccccg
     6421 aggcctacga gcacatgggg gcgatcggga cccggctggc cgagggctgc cgagcggcga
     6481 tcgaggagca cgacatcccc gcgcacaccg tcgacctggg gtgcaagggg tgcgtctcgt
     6541 accggcggga gccgctccgc gactaccgcg acttcctgca ggcgaacggg gagctgttcg
     6601 aggcctcgtg ggcctgggcc atgaaccgcg ggatcttcat gacgccgggc gacgaggagc
     6661 agtggacgct ctcggtccag cactccgagg aagacgtgga ccgctttgtc gaggtcttcg
     6721 ccgagttctg cgccgcggtc gcctcgtgag cgatccggtc ctgctggagg agcagctcgg
     6781 gccggtccgc cgcctgaccc tgaaccgccc gggcgcgctc aacgcgctgt cgagcaagct
     6841 catcgaggag ctgtcggcgg cgctggggcg ggccgccgag gacgacgacg cccgggtcgt
     6901 ggtgatccgg ggagcggggc gggcgttctg cgccggctac gacctcaagg aggagggtcg
     6961 gtcgggcgag atggacgcgg tcgcctggcg gaacgagctg gcccacgacc tgcgtcggat
     7021 gctggagatc ttcgaccacc cccagccggt gatcgccgag gtgcacggct actgcctcgc
     7081 cgggggctgc gacctcatga tgatgtgcga cgtcgcggtg gcctcggacg acgcgaggtt
     7141 cggcgagccc gagatccgct acggcagcgg cgtggtcacc ctcgtcatgc cctggctggt
     7201 cggcgcgcgc aaggccaagg agctcctgct caccggcgag gaccgcgtgc cggccgagga
     7261 ggccctgcgg atcgggctcg tgaacagggt cgtgcccgcc gaccgactgg aggaggagac
     7321 gctcgagctg gcccgcgaga tcgcggtgat ggacccggtc agcatctcgc tgacgaaggc
     7381 cgcgatcaac cgctcgctgg aggcgtccgg cttccgggag gcgctctggg cgaacctcga
     7441 cctcgacgcg ctgatcgagg cggccgaggt gcccgagcgg aaggagttca accggatccg
     7501 ggaggaggag ggcctgaagg ccgcgatccg ctggcgcgac gagcggttcc ggatggagga
     7561 ggggtgacga gcgtgcgggg cctgcggccc ctgcttgccc cgcgatcgat cgccatcgtc
     7621 ggcgcctcgg aggccgaggg ctcctgggcc cccgagttct acgagaacct gcgcgcggcc
     7681 gggttcgagg ggccgatctt ccccgtcaac ccgaagtacc gcgaggtgtg ggggttccgc
     7741 tgtgtgccct cggcggccga tttgccggag ggcgtcgacc tcgcagtgat cgtggtcccc
     7801 gcgcgccacg ccgtctcggc ggtggaggat gcggcgcggg ccggcgtgcg cagcgcgatg
     7861 gtcgtctcga gcgggttcgc cgagtcgggg ggcgagggcg cagcgatgca ggaggagctg
     7921 gcggcggcgg cgcggcgggc cggcatgccc gtgatcgggc ccaacgtcga gggctatgtc
     7981 aacgtcaccg accgcgtctg gtgttacggg acgggccggc cgccgccgct cgcgccggga
     8041 ggcctcacgg tcgtctcgca gagcgggacg gtggcctggt cgctggcgca gatggccggg
     8101 gatcggggtc tgggcgtccg cctcgtgatc ggcgtcggga acgaggcgtc ggtcgggctg
     8161 gccgacatgc tgacctgggc cgccgccgac ccggagacga cggtcgtcgc ctgctacgtc
     8221 gagaccgtgc gcgaccccgc cgggttcgcc ggtgccgcgg acgcgcttcg ggaggcgggg
     8281 aagccgctcc tcgtgtgtgc gcccggccac ggcgagatcg ccggtcgcgc ggtgatcgcc
     8341 cacaccggcg ccgtggccgg gcgcaccggc gttcgcgacg cgtggctgcg ccggctcggc
     8401 gccgccctcg tcccggactc ggtggcgctc ttcgaggccg cggccctggc gtcccgcgcc
     8461 acccggccga agcgccgggg cgtggcggtc gcgttccagt cgggcggcag ctgcacgctg
     8521 tttgccgagc acgcggaggc ggcgggcctc gagatgccgg cgccgtccgc ggccaccgtc
     8581 cggcggatgc gacgggccct gccccccttc gccaagcccg gcaaccccct cgacgtcacg
     8641 ggccaggcct cggtggagac cccgatgttc gagggcgccc tggaggccct ggcccacgac
     8701 cccgcgatcg gggtcgtggc gttcgacgcc ttcccctccc gcgaccccgt ccaggggagc
     8761 tgggagccct cggtgctggc tgccgcagag cggctggcga gggagacggg gacggtgatg
     8821 atctcgctgg cccccgtccc cctggccctc gacgacgcgg gcaggaagtc ggcggagcac
     8881 ctccgcatcc ccgtcctgca gggcatccag gcggcgccca cggcgatccg ggccctgctg
     8941 gacctctccc agacgtctgg aggccgcacc caggccccgc accccgcgcg ccggagggcc
     9001 ggcgccctgc tgcggggacg atccgggccg ctcgacgagc tccgggcccg gaacctgctt
     9061 ggcctctacg ggatcgaggc cccggaggaa gccgtcgccc acacgccaga cgaggccgcc
     9121 cgggccgcgg cgaggctcgg cttccccgtg gccgtgaagg cgctggccgc ggccctcccc
     9181 cacaagaccg cggcgggggc cgtgaaggtc gggctccgcg ccgggcccga ggtcgagcgc
     9241 gccgccgccg cggtcctgcg ggcggcgcgg agggcgggcg tcgcgcccga cggcatcctc
     9301 gtccagcgga tggcctcggg ggccgaggtg ctggtgggcg gggtctggga cgagcgcttc
     9361 ggtcccgccg tgaccgtgaa gccgggcggg gcggcggccg aggcgggcga ggcggagttc
     9421 ctggctgcgc cgctccgcgc gggtgaggcc gcggccttcg tcggccgccg cgccgcccgg
     9481 ctggggctcg agcgccgccg ccatgacctg caggccgttg cggcggcgct cgacgcggtg
     9541 gcccggctcc tcgtcgacct caaggggcgg ctcgtcgagc tcgagacgaa cccgctgatc
     9601 gtctccagga agggcgcggt ggcggtggac gcgctggcgg tcctcagcga gcgggcctga
     9661 agccgcgcag gtcgaggatc gagcccgtcc gccggttcct cggctcgaag tcgggctcga
     9721 cctcgtcgcc aatctgcggc ccctcgtcga agcccacgag ctcgtgcatt agcaccgtgt
     9781 cggccccctc cagccggacg aggccgatcg tccgaggctc ctcgagcggt tcgccctcca
     9841 cccccgcgta gacgacggtg aacgattcga tcctcccccg agggcccacc gtgcggtcgg
     9901 cgtccagctc ccccatgcac cgctcgcaga agatccggtt cgggcagtag acgaccccgc
     9961 agcgctcgca tcccgaggcc aggaactcgc ccttcatcag cgatgcgaag aaggcctccc
    10021 ctgcgacgcc cggcgtgtag cggtgctcca gagggatgta gccctcgacc agccgcacct
    10081 ctttggggtc gctgatcttc tcgaacattc agcccgcctt ccgcctcgcg ccggaccgct
    10141 tccgggacct cgtcttcttc gccggcgact tcctcgccga ggtcttccgg cgggtcccct
    10201 tccgcatcgc ggcgtcccgg ggacggaagt agcggatgtc gaggatcgag ccctcgcgct
    10261 ccccttcggg cttccacacg gcctcgacct ccatgcccac ctcgatcctc tcgggatcga
    10321 cctcgcccag cttgtgcatg aagcccatca tcggcgtgtc gacgtcgagc tccaggacga
    10381 acggcagctc gggctcctcg agctcgacca tgtcccactg gatgtagcag atcgagaacg
    10441 tgttgacggt cccgcgatcg ggcacctcca cccagcggtc ggtggggcgg aagcactcct
    10501 cgcagaacat cctgggcggc acgagcgtcc ggccgcacga gcggcactcg cggcccagga
    10561 tccgtccctc cttcaagccc tccaggaagc ggctgatcgc cacgcccgtg tcccacgcgt
    10621 agcgcgcatc cacggcgaag tccaccgcgc cgatcgcctt gcggaactcc tcgctcttca
    10681 ggtacgtcgg cccgtagaag gtcgtgggct gggtctcgtc cacggcgcgg aggtctcgct
    10741 tcggcgtcat cggcccatca ccaccacggt tcccacctgc atcaggtccc cccaggcctg
    10801 ggccacgccc cggcgtgggt cgcccggcac ctggcgcttc ccggcctggc ccgagatctg
    10861 ccagtagagc tcggcgatct tcatgaggcc ggccgcggcg atcgggttgc ccacgcccag
    10921 caacccgccc gaggggcacg acggcaggtc gccgtcgcgg ttcagcacgc cgtcgcgcat
    10981 cgcctcgggc gccttcccct tctcgaagag ctggagccct tcgaggtggt gcagctcctt
    11041 gtagtcgaac gggtcgtagg gctcggcgat gtggatctcc ttgcgcggct cccggatccc
    11101 cgccatctcg taggcctgcc gggccgcgta ctccacgtag cgcgggtaca cgaggtcgcg
    11161 attcgtccag taggccgtgt cgaggtgcca gcccacgccc tcgacccaga ccggcatcgg
    11221 ggacttcttc ttcgccacgt gctcggccgc cattaccacg gcgaccgcgc cgtcggtcac
    11281 cggcgagacg tcgaggcgct ggacgggcca ggccagcgtc tcgctgttca ggacgtcctc
    11341 cacggtgatg tcggcgtccc cgagcagggc ggcggggtgg tcggccgcgt tccgcttgtt
    11401 cttcaccgag accgccgcga tgtcggcctt gtccagcccg tagacctgca tgtagcggtt
    11461 catctccagc gcgaagatcc acagcaggtt ggggcccagc ggccgctcga tcatgttgtc
    11521 gaagatcgtg atgaacgcgc cctgggggtg gggctgggtc gaggacatct tctcctccgc
    11581 cacgaccagg cacgtgtcga actgccccga tgctacctgc caccagccgt ggatcgcgga
    11641 gaagacgccg gtgccgccgc cgacgtaggt ccggtagtag ggcttccccc aggcgccggc
    11701 gccgtcggcc aggtactccg acttcatgtg gaccccgtcg aaggcgtcgg gggcggtgcc
    11761 catcgtcacg cagtcgatgt cgtcgatcgt catgtcggcc gcctcgagcg cagcccgggc
    11821 cgcctcccag gccagctcct ttcccgtctc ctgcgcccgc cggacgaacc gcgtcatccc
    11881 ggcgccgacg gccgcgaccc gctggcccat caagaagctc ccttcgtgta ctcgatcacc
    11941 tgcaccgtgt tgccgtcggg gtcgaggaag gaagcgaaac gcgcgaagcc ctccacctcg
    12001 cccatgtggt cgaggagctc cacgccgcgc tcctcgagcg cggcccgcgt cgcgtcgagg
    12061 tcatccacct cgaagacggc cgtggcgccg ccggcctccg ccggcctccc ctccaccgca
    12121 ccgtggaggg cgagccggat ggtcccggtc tcgaactccg cccagctcga cccctcctgc
    12181 cggatcagct cgagccccag gacgtcgcga tagaacttca cagctcggtc catgtcctgg
    12241 gtccagtaat agacgtggtc gagtcgccga agccgcggca tcacgccacc gcctcgtcgg
    12301 acgcgaacac cgctacggcc gaggtcgcgg tggggacgcc ccgccacgac tgcgccacgg
    12361 cgaccccggc gtcctcgacc tgccgctccc ccgcctcgcc ccggagctgc agcacgcact
    12421 ccagggcgcg ggccaggccg ttggcttcga agaggttgcc ctggccgagc gagccgcccg
    12481 agacgttgac cgggacatcg ccgtcccgcc cgaagatccc atcctcgagc atcgcgcccg
    12541 cctcgccggg gtgggccagc cgcagggcct cgaggtgcat cagctcgcga tacgcgtacc
    12601 ggtcgtcgac ctcggccagg tccacgaccc ccgtcgggtg gcctatccgc gccatccggt
    12661 acgcacggtc ggcggcctgc cgcgcatcga gggagcggtc ccactcacgg ctctccagcg
    12721 aggccgcgcc ggtggaccag cccgtgccga gcacccagac gggcgcgtcc gtgagatccc
    12781 gggcccggcc ctcgctcgcc agcaccatca cgacgcagcc gtcggcccga tcggccacct
    12841 cgagccgttt caggggccag gccagcggct cggagtcgag cgcgtccgcc gccgtcacct
    12901 cggtggggta ggcggcgcgg gggttgtcca gggccatccg ccggttcttc gccaccacga
    12961 gcgcgcactg ctcctcggtc gtcccggaga cctcgaggta gcggtgcatc tcgaggccgg
    13021 cgatggcgtg cggcgtgacg cggagcggcc ggttgtagac ggggtcgagc gcgaactcgt
    13081 ggatcgagcc gggggtgagg atgtccgagg ccttgctgtg gctctccacg gccacgacct
    13141 cggcgtgccc cgagcggatc agcatcaggg ccgtggcgat cccgaacagc ccgtcggccg
    13201 acacggtgtg gaccgaccgg agggcgccgc cgatctggtc ggggaggtac tcgtcgaaga
    13261 tcgaggtgcc ttcccagaag tcctcggagc acccgacgaa ggagtccacg tcgcggcgga
    13321 cgtccacgcc ggcgtcgtcg taggcgcgca tcgcggcctc gaacatcagc tctttgtacg
    13381 agacgccggc cgtgatcggg gcgaaccccg cgtggccgac gccgacgatc gcgacgcggt
    13441 cgttcatggg cgcgcgcagt ctagcaaagg gccccgagcc accacgaatc cccactcacc
    13501 cgggatgctc ccacagcgcc ccccagcgca gcagccgcgg cccctccccc ctggccagcg
    13561 cgcgcagggc gcggtaggcg ggcgaggagc gcagttcgtc cgcgatcgga agctcgacct
    13621 cgacccggac gatcccagcg ccgtcgtggt gcgccgtccc ccccagctcc tccaggatgt
    13681 cccgcatcgc ccggttgtcc tccaggacgt accccctgaa ccgggtgatc ccgtgctcga
    13741 gggcgctggc gccgagcgcc tgcagcagca gcgagccgag cccccgcccc tggtactcgt
    13801 cgatcacggt gacggccgcc tccgccacct gcgggtcgtc ctcggcgcgc acgtagcgag
    13861 cgaccccgat ccccggccgg ccgggctcgt ccacagccag ggccgcccac gcgaagtggt
    13921 cgacgtagtc caggtcggtc agcttgcgga ccatctcggg gctgagctcg tccaccggcg
    13981 acatgaatct ccggtaccgg ctctcgggcg acaggcggtc gaaggcctcc agcatcttcg
    14041 gcccgtcctc gggcacgagg ggccgcagga gcacccgggt gccgtcggcg aggaccgcct
    14101 cggtggtgcg gcgccggatg aactccgggt ccggggcggt cacagctgct tcaggagccg
    14161 ctgcttgtgg acttcgaact cctggggggt cagcagcccc cgctcgcgca gccgcgccag
    14221 ccgctcgatc tggtcgaggg gtccgggggg gccgtggttg ttcgaggtga cgacacgggc
    14281 gtcctcgatc aggtcgtaga gcgtccgatg gacgtcgtcg gcccggggga tgtaggcgag
    14341 cctggtgaag ccgaggccgc cggccgactc gatcaccacg tcccccaccc gaacgagccg
    14401 ctggaggacc gactggctga agacgacgtc ggccaccccc atgatcggga tctcgacgga
    14461 tcgcttggtc agccagccct gccggtgcac cacccgctcg ttcgtgatga cgtactcgga
    14521 ggtgacccac gccccgatcc tgcggaacgg ccacacgagg aggatcaggg agaacgcccc
    14581 gaccacgatc caccggaccc acgacggcca gctctcgggg atgctgagca gcgccgccgc
    14641 catcccgacc aggaggagga cggtgatgac gagcggcgcg accaccccca tccagtgagg
    14701 gcggagcgca aggacctggt gctcgccctc ggacatccgg ctcggaaagg ccaccccggt
    14761 acctcctcgt gtgtggccgg tccaaggtaa gccgggtcgg gcgtcacggc aagagcgtca
    14821 cgcttcggga cgagtcggca ggccctccgg ggccaggtgg gcgacctcgc cgaaggcgac
    14881 cgcgcgccga ccccggggcg tctcgatcac gagccctccc cgctcgtcga cgtccacggc
    14941 catcccctcg acctcctcac cctcggtcgt ggtggcccgg acggtgcgcc gcagtgagac
    15001 gcaccgctct cgataggcat cgaggagcgc gggggagaac ccctcccgcc cgagcccccg
    15061 ccggaggcgc tccaggtacc ggcggaccag cagggcgggg tgcacggcct ccccctcgac
    15121 ctccaggctc gtggccgttg gccggagctc ctgagggaag tcctcggccc gcatccgcgc
    15181 gttgaccccc gtgcccacga cgacgtggat cacctctccc ccctcgatcc gcgcctcggc
    15241 cagcacgccc cccaccttcc gctccccgat cacgaggtcg ttgggccact tgcaccccac
    15301 ctcggccgtc gtgagctccc ggcaggcatc ggccatcgcc agggcggcca ggagggagat
    15361 caggggtgcc tcggccgggt cgatccgggg ccgcagcagg acgctcatca ggaggccccg
    15421 gcccggcgcc tccacccacc gccgccccag ccgccccttg ccctcggtct ggtgcccggc
    15481 ggccaccacc gtccaggcag gggcgccctc cgcggccagc cgcgcggcct cgacgttcgt
    15541 ggagccgatg ctcgcgtggt atcggggagg ggcctcgatg cctgcggcac ggacggctgc
    15601 gcgcaggcta tcctgactca gcgtctcgga gcccatgggc gggcccgatt atctcaggtg
    15661 gggtctgatg gggaagccac gcgtcctgat cgcgaaccgc ggtgagatcg ccgtgcgggt
    15721 gatccgcacc tgccgggagc tcgacatccc caccgtcgcc gtctattccg acgcggaccg
    15781 cgacgcgctg catgtcgaga tggccgaccg cgcgcaccac atcggcccgg cctcgccggc
    15841 gagctcctac ctgaacgtcg gggcgatcct cgaggcggcc cgcagatccg gggccacgat
    15901 ggtccatccg ggatacgggt tcctggccga gaacttcgac ttcgcccggg cctgcgccga
    15961 ggccgggctc acgttcatcg gtcccccgcc cgaggcgatg gagagaatgg gcgacaaggc
    16021 cgccgcccgc cgggccgccg agcgggcggg ggtccccgtg atccccgggc tctccgaacc
    16081 ggtcgacgcc cgcgaggcgg cccgggtggc cgagcggatc ggcttcccgc tggccgtgaa
    16141 ggccgcgttc ggggggggtg gccgggggat gcacctcgtg gcgacgccgg cggagctgga
    16201 ggaggccgtg gagcgctcct cccgcgaggc ccgggcctac ttcggccgcc ccgaggtcta
    16261 cctcgagcgg tacctggcca gcgcccacca cgtcgaggca cagatcctct gcgatgagta
    16321 cggcaacgcc tcgttcctcg gggagcggga ctgctcgctc cagcgccgct accagaagct
    16381 cgtcgaggag agcccctccc ccgtcgtgga cgagaacctg cgcagcagga tcggcgaggc
    16441 ggcgctggcc atcgccaagg cctccgggta caggaacgcg ggcacggtgg agttcctggt
    16501 cgaggacgac ggctccttct acttcctgga gatgaacacc cggctccagg tcgagcaccc
    16561 ggtgaccgag atggtgaccg gcctcgacct cgtgcggatg cagatcgaag tggccctggg
    16621 cgagaaggtg gacccgggga cccccgacct gcggggccac gcgatcgagt gccgcatcaa
    16681 cgcggaggac ccggccgcgg acttcctccc ccgcccgggg ttcgtgcgcc ggctggtctt
    16741 cccgtcgggg cccttcgtcc gcgtggacgc cgggatcacc gagggccgga cgatccccgg
    16801 cgactacgac tcgctcttcg ccaagctgat cgtctggggc gaggaccgcg aggcggcccg
    16861 ccgccgcatg ctccgggcgc tcgacgagct cgaggtggag ggcgtcccca ccacggtccc
    16921 cttccaccgg tgggtgctga acacgatcgc gttccgcgag gggagccaca ccacgaagtg
    16981 ggtggagcgg gccctggagg aggggatgtt ccggtccgag gaggccgagc ccgcctcgtc
    17041 ggcgcccccc gaccccccgg gccacctcgt ggtcgaggtc ggcgggcgaa gggtgccggt
    17101 ccgggtgtgg ggcgacgacc gcgcagcccc cccgcccgcg cccgcggcgg ccgcccaggc
    17161 gcaccactcc ggcgcccacg gcgcggtcgt cgcccccatg cagggcacga tcctcaaggt
    17221 cctcgtggag gaggggcagg agatcgaggc gggcgacgtg gtctgcatcc tcgaggcgat
    17281 gaagatggag aaccacatcg cggcgaccca ggacgggacg atcagctcgg tggccgtttc
    17341 cgtcggcgac gtcgtgcagg gggggcaggc gatcgccacg atcggctgag cctcagcccg
    17401 ggcggcccca ggcctcgcgg ccggtcaccc tgaagccggc gttccggatg ccgtaggcct
    17461 tgttcactcg gatcagcgcg gccgtgagag gctccaccac cctggccatc gtcagcggcc
    17521 cggcgtccac ccagcgcatc gccatctcct cgacgagctc ccccacggcc gccttcgcct
    17581 ccccgtcccg cccgcagagg agcacgtcgc agtcggggtc gtggtcgagg tcctccagca
    17641 cctcggcgct gaccgtgtgg aagccggaga cgaagcggac gtggcccggg agatacgagg
    17701 ccgcctgctc ggccgccgac ccctgccagg gacggagcac ctgggtcgcg gccgctccca
    17761 ccgccgtagc cagcggcgtc gtcgtgtcgc agacgatcgc cccctcgggc cagcgctcct
    17821 tcagcgcctt gtaggtcggg gcctgcccgg cgtagggcac cgtcacgaag accacgcgag
    17881 cgtcggccac ggcctcgggg ttctccgcgc cctccacggg ggcgtcctcc ccgatgatct
    17941 cggccacccg ggccacggcg tctgcggcct tctccgccga ccgggagccg atgacgaccc
    18001 gacggccgcg cctccccagc ctcagggcga tcccgaaccc gagctcgccg gtccccccga
    18061 cgatggcgac gtgctcgctc accgcgggcg atgctccccg aagaccccgc gcagccggtc
    18121 ggacacctct cccagggtgg cccgcgcctt cagcgcctcc cgcatcgggt agaggaggtt
    18181 gtcggtccca cgggcggcct cctcaacggc cgacagcgtg gcctcgaccg ccggggcatc
    18241 gcgctcctcg cggagggcca ccaaccgctc gacctgccgg gccacggcct cctcgtcgag
    18301 gcgctcgagc tcgacgggct cctcctcgtc ctcggcgtac cggttcaccc ccacgacgat
    18361 ccgctccccc gactccaccc cccgctggat gcggtaggcc gcgtcgtgga tctcgtcctg
    18421 gatccatccg gcctcgatcg cctggaccga gccgcccatc ccgtcgatct tctccaggta
    18481 ctcccaggcg cgcgtctcga tctcgctcgt cagcgactcg acgaagtacg agcccccgag
    18541 cgggtccggc gtgtcgatga ccccggcctc gtgagcgatc acctgctggg tgcggagggc
    18601 gatcctcgcg gcgcgctccg acggcaggga cagcgcctcg tcgaacgagt tcgtgtgcag
    18661 cgactgcgtg ccgccgaaca cggcggccag cgcctcgagc gcggttcgca cgatgttgtt
    18721 ctcgggctgc tgggccgtga gcgtggctcc gccggtctgg gtgtggaagc ggagcatcgc
    18781 cgaccgcggg tccttcgccc cgaaccgctc ggtcatgatc cggtgccaca tccggcgggc
    18841 cgcccggaac ttcgccacct cctcgaagaa gctcatgtgg cacgcgaaga agaaggagac
    18901 gcggggggcg aagtcgtcga cgtc
//
LOCUS       DASUTZ010000274         2926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1536757, whole genome shotgun sequence.
ACCESSION   DASUTZ010000274 DASUTZ010000000
VERSION     DASUTZ010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536757"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..169
                     /locus_tag="VF097_11945"
     CDS             <1..169
                     /locus_tag="VF097_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011096532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-en-1-yl diphosphate
                     synthase"
                     /protein_id="HEX6263547.1"
                     /translation="GPGEAREADLGIAAGKGYGQLIVRGKVQEKRIPESEFVEALVAE
                     AKKLAAETPQA"
     gene            211..1926
                     /locus_tag="VF097_11950"
     CDS             211..1926
                     /locus_tag="VF097_11950"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015944736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX6263548.1"
                     /translation="MRMSELFAHTLREPPGDEEAPSARLLAQGAFVRKLMSGVFTMLP
                     LGLRVLRKVEQIVREEMDAKGAQEMRMPAILPSEPWKVSGRWQAYAAENLMFVLHDRH
                     DRELGLGPTHEEVVTPLVDADVSSYRDLPLNLYHIQWKYRDEARPRSGLIRAREFLMK
                     DAYTFDRDLDGLRAAYEKMVDAYKRIFTRCGLDFRVVEADPGLIGGDVNHEFMAPADV
                     GEDLFVHCTNCDYAANVEAAEAHWPKAPAGEEPKPLEVVPTPGRSTIEAVADLLQVPR
                     HKVLKCLIYKVKDDLVAVLLPGDREVSENKLARLFAPEGVEMLTDADFEKAGLAKGYV
                     GPQGLEGVTVIADHSIRTGANWVAGANEPDAHATGVNLDRDFAVDRWEDLSGVKEGDV
                     CPRCHEGTLMLGRSIEVGHTFQLGTRYSEPLDATFVDEDGSERPFMMGCYGIGVSRII
                     SAVVEQHLDEKGIAWPRAVAPYHVAVIPTNMDDEQVVSVAERLYRELVEAGIEVVIDD
                     RDERAGVKFTDAELIGYPVAVVVGKRGVEAGALEVRFRATGDQREVPVDDAAAALPGL
                     IAEAP"
     gene            1969..2250
                     /locus_tag="VF097_11955"
     CDS             1969..2250
                     /locus_tag="VF097_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263549.1"
                     /translation="MAAKMRSEFRRFAMVANDPEAVIQRIGRDTWDCLVMDVGGTWTR
                     EVFPSQEAAEEACRALEIRYQLGWEDQRLAQRMNRRDHWGEPGGQRRAV"
     gene            2369..2821
                     /gene="rimP"
                     /locus_tag="VF097_11960"
     CDS             2369..2821
                     /gene="rimP"
                     /locus_tag="VF097_11960"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX6263550.1"
                     /translation="MDLDTTIGPVVEGMGLELVEATFRKEGGRRVLRVTVDREGGVDL
                     DTIAEASEKVSRRLDALDFGGDAAYTLEVSSPGVERPLRKPEDFAKRVGEKVKVKTYS
                     PIHGARSHKGEIVAADQEAITIRVEDTERRLAWDDIASARTIFEWGPS"
BASE COUNT          465 a          949 c         1090 g          422 t
ORIGIN      
        1 cgggcccggc gaggctcgcg aggccgacct ggggatcgcg gccgggaagg gctacggcca
       61 gctgatcgtc cgggggaagg tccaggagaa gcgcatcccc gagtcggagt tcgtggaggc
      121 cctggtggcc gaggccaaga agctggccgc cgagacgccg caagcctgat ccgggcggcc
      181 gggcgccggc tccctgtatc ctgacggacc atgcggatgt ccgagctctt cgcccacacg
      241 ctccgggagc ctcccggcga cgaggaggct ccctcagcca ggctgctcgc gcagggggcc
      301 ttcgttcgga agctgatgtc cggcgtgttt acgatgctgc cgctcggtct ccgcgtcctg
      361 cgcaaggtcg aacagatcgt gcgggaggag atggacgcca agggagccca ggagatgcgg
      421 atgccggcca tcctcccatc ggagccctgg aaggtatccg gccgctggca ggcctacgct
      481 gccgagaacc tcatgttcgt cctccacgac cggcacgatc gcgagctcgg gctcgggccg
      541 acccacgagg aggtcgtgac cccgctcgtg gacgccgacg tgtcctcgta ccgtgacctg
      601 ccgctgaacc tctaccacat ccagtggaag taccgggacg aggcccggcc tcgctccggc
      661 ctgatccggg ctcgcgagtt cctgatgaag gacgcgtata cgttcgaccg tgacctcgac
      721 ggactgcgag cggcctacga gaagatggtg gacgcctaca agcggatctt cacgcggtgc
      781 gggctcgatt tccgcgtggt ggaggccgac cccgggctga tcgggggcga cgtcaatcac
      841 gagttcatgg cccccgccga tgtcggcgag gacctgttcg tccactgcac gaactgcgac
      901 tacgccgcca acgtcgaggc agcggaggcc cactggccca aggcccccgc gggggaggag
      961 cccaagccgc tggaggtggt cccaacgcct ggacggtcca cgatcgaggc ggtcgcggac
     1021 ctcctccagg tgccccggca caaggtgctg aagtgcctca tctacaaggt gaaggacgac
     1081 ctcgtcgccg tccttctgcc cggcgatcgc gaggtcagcg agaacaagct cgccaggctc
     1141 ttcgcccccg agggggtcga gatgctcacg gacgccgatt tcgagaaggc gggcctggcc
     1201 aagggctacg tcggtcccca gggcctcgag ggcgtgacgg tgatcgccga tcactcgatc
     1261 cggacgggcg cgaactgggt ggcgggggcg aacgagccgg acgcgcacgc caccggggtg
     1321 aacctggacc gcgacttcgc ggtcgaccgg tgggaggacc tgtcgggggt gaaggagggg
     1381 gacgtctgcc cccgctgtca cgaggggacg ctcatgctgg gtcgctccat cgaggtcggg
     1441 cacacgttcc agctggggac ccgctactcg gagccgctcg acgccacgtt cgtggacgag
     1501 gacggctccg agcggccgtt catgatgggg tgctacggga tcggcgtcag ccggatcatc
     1561 tcggcggtcg tggagcagca cctagacgag aagggcatcg cctggccccg ggccgtcgct
     1621 ccgtaccacg tcgcggtgat cccgacgaac atggacgacg agcaggtcgt gtcggtcgcc
     1681 gagcggctgt atcgagagct cgtcgaagcg ggaatcgagg tcgtgatcga cgaccgcgac
     1741 gagcgggccg gcgtgaagtt caccgacgcc gagctgatcg ggtacccggt cgcggtggtg
     1801 gtcgggaagc gaggggtcga agcgggcgcc cttgaggtcc ggttccgggc gaccggcgac
     1861 cagcgcgagg tccccgtgga cgacgcagcc gccgcgctgc ccggcctcat cgccgaagcg
     1921 ccctgaaggc gcttgcgagc gggcccgacc tcccgtacgg ttcctgcgat ggcggccaag
     1981 atgcgctccg agttccggcg cttcgcgatg gtggcgaacg accccgaggc cgtgatccag
     2041 cggatcgggc gcgacacctg ggactgcctc gtgatggacg tcggggggac gtggacccgt
     2101 gaggtcttcc cctcgcagga ggcggccgag gaggcctgcc gggcgctcga gatacgctac
     2161 cagctcgggt gggaggacca gcggctggcc cagcgtatga accgccggga ccactggggc
     2221 gagccgggag ggcagcgccg ggccgtctga ttccctccgt tcgcgcctct ggcgggggcc
     2281 gtccccgtgc ccgagatcga ctcgctatac tggctccgag ggatcacgtc aagcgtgggc
     2341 gggcgcccac gcttgttgtt tggaggcgat ggacctggac accacgatcg gcccggtcgt
     2401 cgagggcatg ggcctcgagc tcgtcgaggc gaccttccgg aaggaaggtg gccgccgcgt
     2461 gctccgcgtc accgtggacc gcgagggcgg cgtcgacctc gacacgatcg cggaggccag
     2521 cgagaaggtg tcgcggcggc tcgacgcgct cgacttcggc ggggacgcag cctacacgct
     2581 cgaggtctcc tcgccgggcg tcgaacggcc gctgaggaag cccgaggact tcgccaagcg
     2641 tgtgggcgag aaggtgaagg tcaagacgta ttcgccgatc cacggcgccc gatcgcacaa
     2701 gggcgagatc gtagcggccg accaggaggc gatcacgatc cgcgtggagg acacggagcg
     2761 gcggctggcc tgggacgaca tcgcttccgc cagaacgatc ttcgagtggg ggccgtcatg
     2821 aacctggagt tcctcgaggc gctgcgggag ctcgagcgcg agcggggcat ctcgttcgag
     2881 gtgctgctgc acggcatgga ggaggccctg gcctccgcct ggaagt
//
LOCUS       DASUTZ010000275         7214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_760256, whole genome shotgun sequence.
ACCESSION   DASUTZ010000275 DASUTZ010000000
VERSION     DASUTZ010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7214
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760256"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..534)
                     /locus_tag="VF097_11965"
     CDS             complement(1..534)
                     /locus_tag="VF097_11965"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HEX6263551.1"
                     /translation="MRIIAGTAKGTRLAAVPRGTRPLSDRAREGLFSSLGEQVVGARV
                     LDLFAGTGAMALEALSRGAASATLVDSSPDAVRTIRENLTRTGLQGEAVRLDAIRFLS
                     RPIEGFDLVLLDPPWRHPETVVRDVLEALEPHLASGATVAITRPRRASTDVIPVNLQV
                     VKHLDYGETRILVLEAR"
     gene            complement(531..1127)
                     /locus_tag="VF097_11970"
     CDS             complement(531..1127)
                     /locus_tag="VF097_11970"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6263552.1"
                     /translation="MRRRLFSRGYEVFSRAEDRVIAGYRAETAGGARGRVLELGAGNG
                     LNFARYREARVIALEPVADMLPKARARATEAPVPVHLVRGVAEALPFEEDAFDTVVVS
                     LVMCSVPDPAGVAAEIRRVLKRGGQVRFFEHVRAHDPRVARRQDRIGPLYTRFTGGCR
                     MNRDTLTVLRLAGFRIRYRRLYHGPKHLPHVIGVATPS"
     gene            complement(1127..3274)
                     /gene="recG"
                     /locus_tag="VF097_11975"
     CDS             complement(1127..3274)
                     /gene="recG"
                     /locus_tag="VF097_11975"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX6263553.1"
                     /translation="MKITVRTSIASFAGWAKRSVPGLRKLGIATVGDLLHHYPRRYID
                     RSNVASIGDLHVNDDATFIGTVRRVRRAFARNRRQMVTVTVFDPSGSIDLFWFNQSWP
                     AQRFREGMEVAVSGKVGAFRGRLRVTNPAVEILKGGGDETVHVGRIVPVHPAGEGVTT
                     GVIRRLVHDALERVDEVPDPMPADVLARHGLPSLADALRDIHFPETDAAKDRAIERLK
                     LDELFFLELGVAFRKRRVEREAEGIPHDLSGDLAERFVGALPFEITDAQRRTMDEVAK
                     DLAEPRPMHRLLQGDVGSGKTVVALYAALIAIQSGRQAAVMAPTEVLAQQHYRNIRAL
                     LAGIGARSVTEGPLDPQVDLFDPEPGEHNLTYALLTGAVTGAERREVLEGLADGSVQL
                     VVGTHALVQEGVGMRDLSLAVVDEQHRFGLHQRIALKEKGAQPDVLIMTATPIPRTLA
                     LTYYGDLDVSVLDEMPPGRRPVKTRVVRTPQQRRKAYELVRSEVEKGRQAFVVCAAID
                     EGNRLEVKAAEREAERLQTEVFPDLRITLLHGRMRPREKDERMEAFRSGSSDLLIATT
                     VIEVGVDVPNATVMLIENAERFGLAQLHQLRGRIGRAEHESYCILFDESEPDNDDAKA
                     RLQAMARTTDGFELADEDLRLRGEGTLFDIRQSGMPDLKLAQLAQDTDLVRVARRDAF
                     GLIEADPDLSEHPEQRAELERRFAERIDWLFHS"
     gene            complement(3271..4866)
                     /locus_tag="VF097_11980"
     CDS             complement(3271..4866)
                     /locus_tag="VF097_11980"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEX6263554.1"
                     /translation="MGATLDAPTLRRAMTLYARALEAHREEIDSLNVFPVPDGDTGTN
                     MLLTQQAVVEALEGTSDGSLAETGETIARASLMGARGNSGVILSQILRGFCARLGEGE
                     ADGRALAEALARADVEARSAVAEPVEGTILSVLHDAAERAGDGDVGAVAEAAFGAAEA
                     SLERTPDQLMALRDAGVVDAGGKGLVLLLDALRAAIQGDDLRTAVGPPGPVGHREAPA
                     PTGSRYGFETMYLLETANGELGSLREALRRIGDSVVVVGGGGLYNVHVHTDEPDAAVE
                     AGRAIGEPHEVRVTSLDERVARHCLAGQARGAREAEEEEVGRLPLVAVVEGAGLEGIF
                     RSLGATVVRGGPGNNPAVGDLVRALERTPGAVVFVLPNHPNVWPAAEAAARESGRAEV
                     VRTRSAPQGIAAALAYSAGNGSAPMSDEARQTASGRLARAERAAETPGGGVAPGDWVG
                     IDDADGDIWAAGGDAAAVAARLVADRLATDRHELATVYWGAEGTRESAEAVAAAIRAA
                     LPYLETEIQRGDQPRHPYLIALE"
     gene            4923..5429
                     /locus_tag="VF097_11985"
     CDS             4923..5429
                     /locus_tag="VF097_11985"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX6263555.1"
                     /translation="MIARRVVVALVTLLTMACGSTDAGETPRAGPTLHEDRLALPEYD
                     PDRFRALLRTLEGTPVVVNVWASWCGPCRVEGPHLASLAREYEGRVQFLGVDILDNRE
                     AARDFILELDWPYPSVFDPQGEIRDSLGLLGQPVTLVVDEAGEVVFEWSGAVAEDQLR
                     AEIETVIS"
     gene            5457..7148
                     /locus_tag="VF097_11990"
     CDS             5457..7148
                     /locus_tag="VF097_11990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEX6263556.1"
                     /translation="MARPRLSSAAILVCVLRAQAPPAPARQQPPRRVRVAVPEGSSIL
                     VHGEYPPTQSSCVDPVQPVLHERYRGAVEVLRGDDGSLTVIGELGFEVYLKGIAEVPR
                     SWPEEALKAQVVAARSYAASRLDPGGEYDLCATDACQVYMGMGVESGAFGRRWAEAVE
                     ATAGQVLLFQGRPAQTFYSSTSNGRTYTNGEVWGGDPLPYLPSQPERDDGESPVSRWH
                     VEVPFADLERLLRADGRWSGGRLRRVRVEEGEVLLRGRRLGARLSKGELRDALNDTAQ
                     CLLPDRYPPTEPDGTRLPMTVPSTWFRMRTEGRALVLQGRGWGHGVGMVQWGAKGKAD
                     RGLSYEEILAHYYGGLRPQPYDLPGSIRVLLATGLTSVTVEPTGEARIAIRGVGSPPS
                     PPLRVTPTPRGIRVRTSAPSAPRLEIARFGIRPRAGGGIRATIVTESAVKVRAEFLQD
                     DGVVRSRWRSLEAGQVRFRLPAPVPAGPAQVRLRVTDGVDTIVTGTRPVLVEGSATVG
                     PSPSPSPTGAEVSAPMTEPDDRSPSVPLILGLAAGAALVLGLVLTRSRRRGLHRR"
BASE COUNT          951 a         2710 c         2561 g          992 t
ORIGIN      
        1 tcaccgagcc tccaggacga ggatgcgcgt ctccccgtag tcgaggtgct tgacgacctg
       61 caagtttaca ggaatcacat cggtggaagc gcggcgggga cgggtgatcg ctaccgtggc
      121 tcctgaggcc agatgaggct ccagagcctc cagaacgtcc cgaacgaccg tttcggggtg
      181 gcgccacggc ggatccagca gcacgaggtc gaagccctcg atcggacggg agaggaagcg
      241 gatcgcgtcc aagcgcacgg cctctccctg gaggccggtg cgggtcaggt tctcccggat
      301 cgtgcggacc gcatcggggg acgagtccac aagggtggcc gaggcggccc cccgcgacag
      361 cgcctcgagc gccatggcgc ccgtccccgc gaacaggtcg agcacccgtg cgccgacgac
      421 ctgctccccc aggctggaga acaggccctc tcgggcccgg tcggagaggg gccgggtgcc
      481 ccgggggacg gcggcgaggc gcgtcccctt cgccgtgccg gcgatgatcc tcacgacggc
      541 gtggcgaccc cgatcacgtg cggcaggtgc ttcgggccgt ggtacaagcg acggtagcgg
      601 atccggaagc ccgccagccg caggacggtc agcgtgtctc ggttcatccg gcacccgccg
      661 gtgaacctcg tgtagagggg gccgatcctg tcctgacgcc tcgccacccg gggatcgtgg
      721 gctcggacgt gctcgaagaa ccggacctgg ccgccccgct tgaggacgcg ccggatctcg
      781 gccgccacgc ccgccgggtc gggcacgctg cacatcacga ggctcaccac gaccgtgtcg
      841 aaggcgtctt cctcgaacgg cagggcctcg gccacgccgc gcacgaggtg aacggggacc
      901 ggcgcctcgg tggctcgggc tcgcgccttc ggaagcatgt ccgccacggg ctcgagcgcg
      961 atcacccggg cttcgcggta ccgcgcgaag ttgaggccgt tccccgcccc gagctcgagg
     1021 acgcgtcccc gcgccccacc cgccgtctcc gcgcgatagc cggcgatcac ccgatcctcg
     1081 gcccgggaga acacctcgta cccgcgcgag aagagccgcc tgcgcatcag ctgtggaaga
     1141 gccagtcgat ccgctcggcg aaccggcgct ccagctccgc ccgctgctcg ggatgctcgg
     1201 acaggtcggg gtcggcctcg atcaggccga aggcgtcgcg gcgagcgacg cgcaccaggt
     1261 cggtgtcctg cgccagctgg gcgagcttga ggtcgggcat ccccgactgc cgtatgtcga
     1321 acaaggtccc ctcgccccgc agccgcaggt cctcgtcggc cagctcgaag ccgtcggtgg
     1381 tgcgggccat cgcctggagg cgggccttgg cgtcatcgtt gtccggctcc gactcgtcga
     1441 acaggatgca gtacgactcg tgctccgccc ggccgatgcg gccccggagc tggtggagct
     1501 gggccaggcc gaaccgctcc gcgttctcga tcagcatgac ggtcgcgttg ggaacgtcca
     1561 cgccaacctc gatcacggtg gtggcgatga ggaggtccga ggaccccgag cggaacgcct
     1621 ccatccgctc gtccttctcc cgtggacgca tccgcccgtg gaggagggtg atgcggaggt
     1681 ccgggaagac ctcggtctgg aggcgctcgg cctcgcgctc ggcggccttc acctcgaggc
     1741 ggttcccctc gtcgatcgcg gcgcacacga cgaaggcctg gcggcccttc tccacctcgg
     1801 agcgcacgag ctcgtaggcc ttccgccgct gctgcggcgt ccggaccacc cgggttttga
     1861 cggggcggcg gccgggcggc atctcgtcga gcaccgacac atccaggtcg ccgtagtacg
     1921 tgagcgccag cgtccgcggg atcggcgtgg cggtcatgat caggacgtcc ggctgggcgc
     1981 ccttctcctt cagcgcgatc cgctgatgga ggccgaagcg gtgctgctcg tccacgaccg
     2041 ccagcgagag gtcccgcatc ccgaccccct cctggacgag cgcgtgcgtg cccacgacga
     2101 gctggacgct gccgtcggcc aggccctcga ggacctccct ccgctcggcg cccgtgacgg
     2161 cgccggtgag gagcgcgtac gtgaggttgt gctcgcccgg ttccgggtcg aagaggtcga
     2221 cctgtgggtc gagcgggccc tcggtgaccg accgggcacc gatcccggcg agcagggccc
     2281 ggatgttccg gtagtgctgc tgggccagga cctcggtggg ggccatcacc gcggcctggc
     2341 ggcccgactg gatcgcgatc agcgccgcgt agagggcgac gacggtcttc cccgagccca
     2401 cgtccccctg gaggaggcgg tgcatcggcc gaggctcggc caggtccttc gccacctcgt
     2461 ccatcgtccg gcgctgggcg tccgtgatct cgaagggaag ggcgccgacg aagcgctcgg
     2521 ccagatcgcc ggagaggtcg tgggggattc cctcggcctc gcgctccacc cggcgcttgc
     2581 ggaaggccac ccccagctcc aggaagaaga gctcgtccag cttcagccgc tcgatcgccc
     2641 ggtccttcgc cgcgtcggtc tccgggaagt ggatgtctcg gagggcgtcg gccagcgagg
     2701 gcagcccgtg ccgcgccagc acgtcggccg gcatcggatc ggggacctcg tcgacgcgct
     2761 ccaaggcatc gtggaccagg cggcggatca cgccggtggt gacgccttcg cccgcggggt
     2821 ggacgggaac gatccggcct acgtggacgg tctcgtcgcc cccgcccttg aggatctcca
     2881 ccgcggggtt ggtgacccga agcctcccgc ggaaggcgcc caccttgccc gagaccgcca
     2941 cctccatccc ctcgcggaac cgctgggcgg gccaggactg gttgaaccag aagaggtcga
     3001 tcgagccgct ggggtcgaag acggtgacgg tgaccatctg ccggcggttc cgggcgaagg
     3061 cgcggcgcac gcggcggacg gtcccgatga acgtggcgtc gtcgttcacg tggaggtcgc
     3121 cgatcgaggc cacgttggag cggtcgatgt agcggcgcgg gtagtggtgg aggaggtcgc
     3181 cgacggtcgc gatcccgagc ttgcggagcc cgggcacgga tcgcttcgcc cacccggcga
     3241 aggacgcgat cgaggtcctg acggtgatct tcattccagc gcgatcaggt agggatggcg
     3301 gggctggtcc ccccgctgga tctcggtctc aaggtacggg agcgccgcgc ggatcgccgc
     3361 ggccaccgcc tccgcgctct cccgggtgcc ctccgctccc cagtagacgg tggccagctc
     3421 gtgacggtcg gtggccagcc ggtcggccac gaggcgtgcc gccacggcgg cggcgtcgcc
     3481 cccggccgcc cagatgtctc cgtctgcatc gtcgatcccc acccagtccc cgggggcgac
     3541 gccgccgccg ggcgtctcgg ccgctcgctc ggcccgggcc agccgtcccg aggccgtctg
     3601 ccgggcctcg tccgacatgg gggccgagcc gttccccgcc gagtacgcga gcgccgcggc
     3661 gatgccctgg ggagccgacc gggtgcgcac gacctccgcg cggccggact cccgggccgc
     3721 ggcctcggcg gccggccaca cgttggggtg attggggagc acgaagacca cggcccccgg
     3781 cgtccgctcc agcgcccgga ccaggtcgcc gacggccggg ttgttcccgg gacctccccg
     3841 tacgaccgtc gcccccagcg agcggaagat gccctccagg cccgctccct ccaccacggc
     3901 caccagaggc agccggccca cctcctcctc ttcggcctcg cgagctcccc gagcctggcc
     3961 cgcgaggcag tgccgggcca cccgctcgtc gagagacgtg acccggacct cgtggggctc
     4021 gccgatcgcg cgacccgcct cgaccgcagc gtccggctcg tcggtgtgca cgtggacgtt
     4081 gtacaggccg ccgccgccca ccacgacgac cgaatccccg atccggcgga gtgcctcgcg
     4141 cagggatccc agctccccgt tcgcggtctc cagcaggtac atcgtctcga acccgtacct
     4201 cgatcccgtg ggcgccggcg cctcgcgatg gccgacgggg ccgggcggcc cgaccgccgt
     4261 ccgaaggtcg tccccctgga tcgccgcgcg cagggcatcg aggagcagca cgagcccctt
     4321 cccgccggca tccaccaccc cggcgtcgcg cagggccatg agctgatcgg gggtgcgctc
     4381 cagcgaggcc tccgcggcgc cgaacgccgc ctccgccacg gccccgacgt ccccgtcgcc
     4441 cgcccgctcg gcggcgtcgt ggagcacgga taggatcgtc ccctccaccg gctcggccac
     4501 cgcgctcctc gcctcgacgt cggcccgcgc cagcgcctcg gccagcgccc ggccgtcggc
     4561 ctcgccctcg ccgaggcggg cgcagaagcc ccggaggatc tgcgagagga tcacgcccga
     4621 gttcccccga gcccccatca gcgaggcgcg ggcgatcgtc tcccccgtct ccgccagcga
     4681 tccgtcgctt gtcccttcca gcgcttccac caccgcctgc tgggtgagca gcatgttggt
     4741 cccggtgtcc ccgtcgggca cggggaagac gttcagcgaa tcgatctcct cccggtgagc
     4801 ctccagagcc cgtgcgtaga gggtcatcgc ccgccgcagc gtcggcgcgt cgagggtcgc
     4861 tcccacctcg ctcacatcct atccctcgac cccgccccca ccggccggtg taccgtgagg
     4921 gcatgatcgc ccggcgcgtg gtcgtggccc tcgtgaccct cctgaccatg gcctgcggga
     4981 gcaccgacgc cggcgagacc ccccgagccg ggcccacgct ccacgaggac cgcctcgctc
     5041 tccccgaata cgatcccgac cggttccgcg ccctcctccg gaccctcgag ggcacaccgg
     5101 tcgtggtgaa cgtgtgggcc tcgtggtgcg ggccgtgccg ggtggagggc ccgcacctcg
     5161 cgtcgctggc tcgcgagtac gagggccgcg tccagttcct gggggtggac atcctcgaca
     5221 atcgcgaggc cgcccgcgac ttcatcctcg agctcgactg gccctacccc agcgtcttcg
     5281 accctcaggg ggagatccgc gacagcctgg ggctcctggg ccagcccgtc acgctggtcg
     5341 tggacgaggc cggcgaggtc gtcttcgaat ggagcggcgc ggtggccgag gaccagctca
     5401 gggccgagat cgagaccgtg atctcctagc gcgaggccgg tgctaccgtg ggtcgcatgg
     5461 cccgcccacg gctgtcgtcg gccgcgatcc tggtctgcgt cctgcgggcc caggccccgc
     5521 cggcacccgc ccggcagcag ccaccgcgcc gcgtcagggt ggccgtgccg gagggctcct
     5581 cgatcctcgt ccacggcgag tacccgccga cccagtcctc gtgcgtggat cccgtccagc
     5641 ccgtcctcca cgagcgatac cgcggagcgg tggaggtgct ccggggcgac gacggctcgc
     5701 tcaccgtgat cggcgagctg gggttcgagg tctacctcaa gggcatcgcc gaggtcccgc
     5761 gatcctggcc cgaggaggcg ctgaaggcgc aggtcgtggc ggcgcgctcg tacgccgcca
     5821 gccgcctcga ccccggcggc gagtacgacc tctgcgccac cgacgcctgc caggtctaca
     5881 tggggatggg ggtcgagagc ggggcgttcg gaaggcggtg ggcggaagcc gtcgaggcca
     5941 ccgcgggcca ggtcctcctc ttccagggga ggcccgcgca gaccttctac tcctcgacct
     6001 cgaacggcag gacctacacc aacggggagg tctggggcgg cgacccccta ccctacctcc
     6061 cctcccagcc cgagcgggac gacggcgagt cgccggtctc ccgctggcac gtggaggtcc
     6121 ccttcgccga cctagagcga ctcctcaggg cggacgggcg gtggagcggg ggtcgcctcc
     6181 ggcgcgtccg cgtcgaggag ggtgaggtcc tcctccgggg ccggcggctc ggcgcccgac
     6241 tgagcaaggg cgagctccgc gacgccctga acgacacggc ccaatgtctc ctccccgacc
     6301 gctacccgcc caccgagccc gacgggacga ggctccccat gacggtccca tcgacgtggt
     6361 tccgcatgcg gaccgagggc cgggccctgg tgctccaggg ccgcggatgg ggacacggcg
     6421 tggggatggt gcagtggggg gcgaagggga aagccgaccg cggcctctcc tacgaggaga
     6481 tcctcgccca ctactacggg ggcctgcggc cccagccgta cgaccttccg ggctcgatcc
     6541 gcgtgctcct cgcgacgggg ctgacctcgg tgacggtaga gcccacgggc gaggcgcgga
     6601 tcgcgatccg aggggtgggc agcccaccct ccccccctct cagggtcacc ccgacgcccc
     6661 gggggatccg tgtgcggacc tccgcgccct ccgccccgcg attggagatc gcgcgcttcg
     6721 ggatccggcc tcgggccggc ggcgggatcc gcgccacgat cgtcaccgag tcggcggtca
     6781 aggtccgggc cgagttcctc caggacgacg gggtcgtgcg gagccgctgg cgctcgctcg
     6841 aggccgggca ggtgcggttc cggctccccg ctcccgtgcc ggccggcccg gcgcaggtgc
     6901 gcctccgggt caccgacggc gtggacacga tcgtgaccgg cacgcggccg gtgctcgtgg
     6961 agggctccgc caccgtggga ccgagcccgt ccccgtcccc gaccggggcc gaggtgtcgg
     7021 ccccgatgac cgagcccgac gaccgaagcc catccgtccc gctgatcctc ggtctcgccg
     7081 ccggggcggc cctcgtcctg ggcctcgtgt tgacccggag ccgccgcagg gggttacatc
     7141 gaagatgatg ccgctcgtcg ccgagcacgt gccgatcatc tccctcgact ggctgaccag
     7201 ccacgggctg atca
//
LOCUS       DASUTZ010000276         2508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1148284, whole genome shotgun sequence.
ACCESSION   DASUTZ010000276 DASUTZ010000000
VERSION     DASUTZ010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148284"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VF097_11995"
     CDS             complement(<1..516)
                     /locus_tag="VF097_11995"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015578346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HEX6263557.1"
                     /translation="MLHIPLYQSELNQIWGPPRSLPGVGLIKGLFVTLKHLLQPSITQ
                     QYPREKPELPARTRGVIALKPENCTVCWKCSKECPDWCIYIESHKETHEPATGGRARS
                     QKVLDRFAIDFALCMYCGICVEVCPFDALFWSPEFEYAEYDVLELLHEKDRLRDWTYK
                     VKPPPELEWGAE"
     gene            complement(992..1969)
                     /gene="nuoH"
                     /locus_tag="VF097_12000"
     CDS             complement(992..1969)
                     /gene="nuoH"
                     /locus_tag="VF097_12000"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018834944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX6263558.1"
                     /translation="MSFFDQLFGNVWVVLALKVVVVMVFFLTAPMAIGYTEHKVLAHM
                     QHRLGPMEAGRFHGWAQLIADGVKFIQKEDVVPSAADRWVFSLAPAVFMIPVVLVFAV
                     IPFGQDVWAENLDVGLFFVMAISAISVIGVLMAGWSSANKFSLIGALRAAAQLIAYEL
                     PLILAAVAVAMLAGTLSLVGIVEAQRSFWYVLVQPVGLLIFLVASIAELTRPPFDMPI
                     ADSEIIFGAYTEYTGMKFAFFLLAEYAGIVALSAVASVLYLGGYLGPFLPAPIWMLLK
                     IGGLSFFIIWLRATYPRLREDQLQRFSWIVLIPLALANILVTAIVKVVV"
     gene            complement(1966..>2508)
                     /locus_tag="VF097_12005"
     CDS             complement(1966..>2508)
                     /locus_tag="VF097_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018736858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX6263559.1"
                     /translation="RKTSGRSGVASSPPLPARDLIDRLRAQLGDDLTESSDQHGQAAI
                     GVPSARWRETIEWLRDDDELAFDFFDFLTAVDRRDEGFDVVVQLWSTTHRHQVRVKVR
                     LPRDNPRLPSIHDLFPGADWYERETWELFGIDFEGHPGLVKLVLPEEFEGHPGRKDFP
                     LATRIAKPWPGDEQIGEVEE"
BASE COUNT          446 a          858 c          859 g          345 t
ORIGIN      
        1 ctcggccccc cactcgagct cgggcggcgg cttcaccttg taggtccagt cccgcagccg
       61 gtccttctcg tgcagcagct cgaggacgtc gtactccgcg tactcgaact cggggctcca
      121 gaagagcgcg tcgaacgggc acacctccac gcagatcccg cagtacatgc agagcgcgaa
      181 gtcgatggcg aaccggtcca ggaccttctg cgagcgggcc cggccgcccg tcgccggctc
      241 gtgcgtctcc ttgtgcgact cgatgtaaat gcaccagtcc gggcactcct tggagcactt
      301 ccagcagacc gtgcagttct cgggcttgag cgcgatcacg ccgcgggtcc gggccgggag
      361 ctcgggcttc tcccgagggt actgctgggt gatcgagggc tggaggaggt gcttcagggt
      421 cacgaagagg cctttgatga gcccgacgcc cggcagagag cggggcggcc cccagatctg
      481 gttgagctcg gactggtaga gcgggatgtg gagcatggcc ggcgagacgg ccacgcccag
      541 gagcggggag acgctgggct cgagccccgc gcgctcctgc atcagggcga tgcgctcgcc
      601 ggtgcggaag accgagacga tcagcgggat ggggatgatg agcagggccc ccaccccgag
      661 cagccacgtc aggaggctca cgctcgtccc cgtgatgacc aggacgagca tcacgatcag
      721 gggcacgggc gcggccagcc cgatgatcac ggccagggcc gaaagggcgg gggcgatcct
      781 ccggtccccc aggtaccgcc gcgcctcgtc gttcaccttg tactgccaca ccacccagta
      841 gaggcccagc gagatgaggg ccaggagcca agccccgacc gggttgcgcc gccggtaccg
      901 ctggccgccc aggtcgacgt gcctggcgtc gtcgggcgga gcgggcgcgg ggctcacccg
      961 cgcctcgtcc tcgcggacgt ccacggccac ctcagaccac caccttcacg atcgccgtca
     1021 ccaggatgtt cgccagggcc agcgggatca gcacgatcca ggagaagcgc tggagctggt
     1081 cctcgcgcag ccgcgggtac gtggctctga gccagatgat gaagaatgag agcccgccga
     1141 tcttcagcag catccagatc ggcgcgggga ggaacggccc gaggtagccg cccagataca
     1201 gcaccgaggc aaccgccgac agcgccacga tcccggcgta ctcggccagc aggaagaagg
     1261 cgaacttcat ccccgtgtac tccgtgtagg ccccgaagat gatctcggag tccgcgatcg
     1321 gcatgtcgaa gggcggccgg gtcagctccg cgatcgacgc gaccaggaag atgaggagcc
     1381 ccaccggctg gacgagcacg taccagaagc tgcgctgggc ctccacgatc cccacgagcg
     1441 agagggtccc cgccagcatc gccaccgcca ccgccgccag gatcaggggc agctcgtagg
     1501 cgatcagctg agccgcggcg cggagagccc cgatcagcga gaacttgttg gccgaggacc
     1561 agcccgccat caggaccccg atcacggaga tggcggagat cgccatcacg aagaagaggc
     1621 cgacgtcgag gttctcggcc cacacgtcct gcccgaaggg gatcacggcg aacacgagga
     1681 ccacagggat catgaacacg gccggggcca gcgagaacac ccagcgatcc gccgcggagg
     1741 ggacgacgtc ctccttctgg atgaacttca ccccgtcggc gatcagctgg gcccagccgt
     1801 ggaaccgccc cgcctccatc ggccccagac ggtgctgcat gtgggccagg accttgtgct
     1861 ccgtgtagcc gatcgccatc ggggcggtca ggaagaagac catgaccacg acgaccttga
     1921 gggcgagcac cacccacacg ttgccgaaga gctggtcgaa gaagctcact cctcgacctc
     1981 cccgatctgc tcgtccccgg gccagggctt ggcgatccgc gtggcgaggg ggaagtcctt
     2041 cctcccgggg tgcccctcga actcctcggg caacacgagc ttgaccagcc cggggtggcc
     2101 ctcgaagtcg atcccgaaga gctcccaggt ctcgcgctcg taccagtcgg cccctgggaa
     2161 gaggtcgtgg atgctgggga ggcgggggtt atcgcggggc aggcgcacct tcacccgcac
     2221 ctggtggcgg tgggtggtcg accagagctg gacgaccacg tcgaagccct cgtcacgccg
     2281 gtcgaccgcc gtgaggaagt cgaagaagtc gaaggcgagc tcgtcgtcgt cccggagcca
     2341 ctcgatcgtc tcgcgccagc gagcggacgg cacgccgatc gccgcctgcc cgtgctggtc
     2401 cgaggactcc gtgaggtcgt ccccgagctg agcccgcagg cggtcgatca ggtcacgtgc
     2461 tggaaggggc ggtgagcttg ccactccact tctcccggat gtcttcct
//
LOCUS       DASUTZ010000277         6851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1277797, whole genome shotgun sequence.
ACCESSION   DASUTZ010000277 DASUTZ010000000
VERSION     DASUTZ010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6851
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277797"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..1379)
                     /gene="hemL"
                     /locus_tag="VF097_12010"
     CDS             complement(84..1379)
                     /gene="hemL"
                     /locus_tag="VF097_12010"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX6263560.1"
                     /translation="MPGRSEELFQRARAVIPGGVSSPVRAFGAVGGVPRFAASGEGAW
                     VEDADGVRRVDYVQSWGALLFGHARREIVEAAREAAARGTSFGMPTEGEVRLAEALRA
                     AMPSLEMVRLVSSGTEAAMSAVRLARASAGRDRIVKFAGCYHGHSDGLLASAGSGLAT
                     LGIPGTPGVTEGASRDTVVLPYNDRQAAAETFEEVGDEIAAVIVEPVAANMGVVPPEP
                     GFLEDLRDLTIRSGALLIFDEVITGFRVGRGGAQERFGVAPDLTVLGKVMGGGMPIAA
                     FGGRAEILERLAPAGDVYQAGTLSGNPVAVAAGLAALALIERRDPYPALEKAAERLVE
                     GLASILEDAGIPFTINRAASLFSLYFGVEGVRDHAQAAAADHEAYARFFHGMLEAGVA
                     LPPSGYEAWFLGTAHGDDEIGRTLEAAASAAERVSAPSR"
     gene            complement(1379..2350)
                     /gene="hemB"
                     /locus_tag="VF097_12015"
     CDS             complement(1379..2350)
                     /gene="hemB"
                     /locus_tag="VF097_12015"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017603124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX6263561.1"
                     /translation="MAFPQDRPRRLRRTTALRDLVRETDLAPRHLVAPLFVKEGIAEP
                     VAISSMPGHHQHTLESLRKEARELAEKGIRALVLFGIPGRKDPEGSEGWADAGVSQRA
                     IRALREELGDEVVLIADLCLCEYTDHGHCGVLEGEELDNDATLERYRRIAVAQAAAGA
                     DLVAPSGMMDGQVAAIREALDQGGFPGTAILAYAAKYASSFYGPFREAAEGAPRFGDR
                     RSHQMDPGNADEALREIRADVAEGADLVMVKPALPYLDVIHRARDAVDVPVAAYNVSG
                     EYAMIKAAAEQGWIDGTAAMLETLTAIRRAGAGLILTYFAREAAELL"
     gene            complement(2376..3131)
                     /locus_tag="VF097_12020"
     CDS             complement(2376..3131)
                     /locus_tag="VF097_12020"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX6263562.1"
                     /translation="MTALEGRTVVVTRSADRVEELAALLRERGAVPLPAPAIRIEPVP
                     EGGPLDEAITDVAMRGYAWVVFTSGAGVDAWFARAAELGVDARALNAKVAAVGSGTSE
                     RLRDHGIESELVPPEFTTASLAETFPEGREKVLLARADIATPELEESLRGKGWQVTRV
                     DAYRTIPADELPEAVAQALGEGRVDAVTFTSRSTVQGFTALAEIADGPLVACIGPVTA
                     DAARAAGLRVDAVASPHTLEGLVEALERAFASR"
     gene            complement(3128..4015)
                     /gene="hemC"
                     /locus_tag="VF097_12025"
     CDS             complement(3128..4015)
                     /gene="hemC"
                     /locus_tag="VF097_12025"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX6263563.1"
                     /translation="MRIRIGTRRSRLALAQASEVAELLGGSGHEAELVPMTTAGDRGA
                     PAEASPQGTKGLFVAEITEALLTGDVDLAVHSAKDLPSDDPEGVVVAAVPRRSLPYDV
                     LATATAGFDEEGTVGTSSLRRQAQLLRAYPSARVVPFRGNVDTRLRKLRQGEVDAVVL
                     AAAGLDRLGGRPAHAEPIPVDVMVPAPGQGALAVQARAEDAVAEAVASLDHASSRRAF
                     EVERALVSALGGGCRLPLGAYAEARSGVVRLLAVVARPDGSELLRAEVEGDDPGEVAT
                     HAAEQLRAAGADAILREAS"
     gene            complement(4015..5280)
                     /locus_tag="VF097_12030"
     CDS             complement(4015..5280)
                     /locus_tag="VF097_12030"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:NF000744.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX6263564.1"
                     /translation="MPILALGVSYRRASVDVLERLAWGDEDLPKAYDHVRSMPSVEEA
                     VLLSTCNRVEVYGAVEGYHTGFQDLKRFLSESGGVEPEEFAEPLYSHYEDHTAEHLFS
                     VAAGLDSMVVGEPQILTQVRQAFQRARGEEAAGPQLSALFRYAIRAGRRARAETKIGA
                     SPSAFVEAGGGAAARHLGGLGGRSVLLVGAGEMAALALGWLRRQGVGEVTVLNRTPEK
                     AQRLAVRVGGSHGGLEELSDALARTDLVVSSTTATQVVVGREDVERAAGGRRMFLLDL
                     AVPRDVDPAAREVPGVRVADIDDLRDVVAASREGIADEIEAAGRVIAEETRKFVTQRR
                     AAKLAPLIAALHERGEEVRAAELERLASRLSGLSDREREAVETLTKGIVKTLLHDPVV
                     ELKERWGAGVGEAHARVLAELFGLEPPKD"
     gene            complement(5293..6045)
                     /locus_tag="VF097_12035"
     CDS             complement(5293..6045)
                     /locus_tag="VF097_12035"
                     /inference="COORDINATES: protein motif:HMM:NF003992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEX6263565.1"
                     /translation="MENPPVPSYRARSRTHERRMTKRPIPEATVGRLPVYLRVLVEMA
                     EDGRSTVSSEALAEAAGVNSAKVRKDLSYLGSYGTRGVGYDVAYLIHQVRRELGLTQD
                     WPVLIAGVGNLGQALANYRGFDARGFRVAGLVDPARVGERVAGVEVRPPEEMPDLVRE
                     HGIQIGMICTPGPAAQEVADRMVEAGIRSILNFSPVVIACPEGVSLRKVDLSVELQIL
                     AFYEQRKAVLDEVRRGEALPQSLGETAERPTA"
     gene            complement(6055..6288)
                     /locus_tag="VF097_12040"
     CDS             complement(6055..6288)
                     /locus_tag="VF097_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX6263566.1"
                     /translation="MAEVVMYARPGCHLCDEARETILLLREELGFDYREVDVEGDPEL
                     EREHGLRVPVVTVDGEEAFEHRVDADELRRLLS"
     gene            6333..>6851
                     /locus_tag="VF097_12045"
     CDS             6333..>6851
                     /locus_tag="VF097_12045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6263567.1"
                     /translation="MAEADDELARRVADRDPEAYEQIYRRYAAAAYGVALRVLRDPAL
                     AQEVAAESFLSLWSTPEAYDPSRGRFRSFFLSLVHHRAVDAVRREERLRERERKVNRA
                     PLPDEDWSEAVVEEADLVDRRREVREALSELPPEQREALELMYFRGYTQSRIAEERGI
                     PLGTVKTRVHAAM"
BASE COUNT          921 a         2653 c         2382 g          895 t
ORIGIN      
        1 caccatcgtg ctggcgatcg aggagggcgg gctctcgttc atcctcttcg ccctcatgct
       61 cgtggtgatc ttcgtcgtgt tcctcatctc gatggcgcgc tgacgcgctc ggcggccgag
      121 gcggccgcct ccagggtccg cccgatctcg tcgtcgccgt gtgccgttcc caggaaccaa
      181 gcctcgtacc ccgacggcgg gagggccacg ccggcctcca gcatcccgtg gaagaacctg
      241 gcgtaggcct catggtcggc ggcggccgcc tgcgcgtggt cgcgaacgcc ctccaccccg
      301 aagtacagcg agaagagcga ggccgcccgg ttgatcgtga aggggatgcc cgcgtcctcg
      361 aggatcgagg ccaggccctc cacgaggcgc tcggcggcct tctccagcgc cgggtaggga
      421 tcacggcgct cgatcagggc cagcgcggcg aggccggcgg ccaccgccac ggggttcccg
      481 gagagggtcc ccgcctggta gacgtcgccg gcgggggcca gccgctccaa gatctcggca
      541 cggccgccga aggcggcgat gggcatcccg ccgcccatga ccttgcccag cacggtgagg
      601 tcgggcgcca cgccgaagcg ctcctgcgcg ccgccccggc ccacccggaa ccccgtgatg
      661 acctcgtcga agatcaagag ggcaccggat cgaatcgtga gatcgcgaag gtcctccagg
      721 aaccccggct cgggcggaac cacgcccatg ttggccgcca ccggctccac gatgacggcc
      781 gcgatctcgt cgccgacctc ctcgaaggtc tcggctgcgg cctggcggtc gttgtagggc
      841 agcaccacgg tgtcgcggga ggcgccctcg gtgacccccg gcgtgcccgg gatccccaac
      901 gtggccaggc ccgagccggc gctggccagc aggccgtccg agtgaccgtg gtagcagccg
      961 gcgaacttca cgatgcggtc gcggccagcg ctggctcggg ccagccgcac cgcgctcatc
     1021 gcggcctcgg tcccgctgga gacgagccgg accatctcca gcgagggcat cgccgcgcgg
     1081 agggcctcgg ccagccgcac ctcgccctcg gtcggcatcc cgaacgaggt gccgcgggcg
     1141 gcggcctccc gggcggcctc cacgatctct cgtcgagcgt gcccgaagag cagggcgccc
     1201 cacgactgca cgtagtcgac gcgtcggacg ccgtcggcgt cctcgaccca ggccccctcg
     1261 cccgaggcgg cgaagcgcgg gacgccgccg acggccccga aggcgcggac cggcgagctc
     1321 acccccccgg ggatcaccgc gcgggcacgc tggaacagct cctcggatcg gcccggcatc
     1381 agagcagctc ggcagcctcg cgggcgaagt aggtgaggat gaggccggcc ccggcgcgac
     1441 ggatcgccgt gagcgtctcc agcatggcgg cggtgccgtc gatccatccc tgctcggcgg
     1501 cggccttgat catcgcgtac tcgcccgaga cgttgtaggc ggccaccggc acgtcgacgg
     1561 cgtcgcgtgc ccggtggatc acgtcgaggt acgggagggc cggcttcacc atgaccaggt
     1621 ccgccccctc ggccacgtcg gcccggatct cgcgcagcgc ctcgtcggcg ttgcccgggt
     1681 ccatctggtg ggagcggcga tcgccgaacc gcggggcacc ctcggcggcc tcgcggaagg
     1741 gcccgtagaa gctcgaggcg tacttggcgg cgtaggcgag gatcgccgtc cccgggaagc
     1801 ctccctgatc cagcgcctcg cggatcgcgg ccacctggcc gtccatcatc cccgacggcg
     1861 cgacgagatc ggctcccgcc gcggcctggg ccaccgcgat ccgccggtat cgctccagcg
     1921 tggcgtcgtt gtcgagctcc tcgccctcga ggacgccgca gtgcccgtgg tccgtgtact
     1981 cgcagaggca gaggtcggcg atcagcacga cctcgtcccc cagctcttcc cggagcgcgc
     2041 ggatggcccg ctggctgacc ccagcgtcgg cccacccctc gctcccctcg gggtccttgc
     2101 gtccagggat cccgaagagg acgagcgccc ggatcccctt ctcggcgagc tcgcgggcct
     2161 ccttccggag ggactcgagg gtgtgctggt ggtggccggg catcgaggag atggcgacgg
     2221 gctcggcgat cccctccttg acgaagagcg gggcgacgag gtggcggggg gcgaggtcgg
     2281 tctcgcggac gaggtcgcgg agcgcggtgg tcctgcggag ccgcctcggc cggtcctggg
     2341 ggaacgccat ggatcgaatg gtagagcgtt tccggctacc gcgaggcgaa ggcccgctcc
     2401 agcgcctcga ccaggccctc gagcgtgtga ggcgaggcca cggcgtccac gcggaggccg
     2461 gcggcccgcg cggcgtcggc ggtgaccggg ccgatgcagg ccacgagcgg gccgtcggcg
     2521 atctctgcca gcgccgtgaa gccctggacg gtcgatcggg aggtaaaggt cacggcgtcg
     2581 acgcgaccct cccccagggc ctgggccacc gcctcgggca gctcgtccgc ggggatcgtg
     2641 cggtaggcgt ccacccgcgt cacctgccag cccttgccgc ggagcgactc ctcgagctcg
     2701 ggggtcgcga tgtcggcccg ggccagcagg accttctccc tgccctccgg gaaggtctcg
     2761 gccagggagg ccgtggtgaa ctcgggcggg acgagctcgg actcgatccc gtggtcccgg
     2821 agtcgctccg aggtgccgct cccgacggcg gcgaccttgg cgttcagggc gcgggcgtcg
     2881 acgcccagct ccgcggcacg ggcgaaccac gcgtcgacgc ccgcgcccga cgtgaacacc
     2941 acccacgcgt agccgcgcat ggcgacgtcg gtgatcgcct cgtccagcgg gccgccctcc
     3001 gggaccggct cgatccggat cgccggcgcc gggagcggca cggcccctcg ctcccgcagc
     3061 agcgcggcga gctcctccac ccgatccgcg ctccgcgtga cgaccaccgt tcggccctcg
     3121 agcgccgtca cgaggcctcc cggaggatcg cgtccgcacc ggccgcccgc agctgctccg
     3181 cggcgtgtgt cgccacctcg ccggggtcgt cgccctccac ctcggctctg aggagctcgc
     3241 tcccgtccgg gcgggccacc acggccagca ggcgcaccac cccgctgcgg gcctccgcat
     3301 acgccccgag cgggaggcgg cagcccccgc ccagcgcact caccagggcc cgctccacct
     3361 cgaaggcgcg gcgcgaggag gcatggtcca gagaggcgac cgcctcggcg acggcgtcct
     3421 cggccctggc ctggaccgcg agcgccccct ggccgggggc cgggaccatg acgtccacgg
     3481 ggatcggctc ggcgtgcgcg gggcgcccgc cgagccgatc cagccccgcg gcggccagca
     3541 ccaccgcgtc cacctcgccc tggcgcagct tccgcagccg cgtgtccacg ttcccccgga
     3601 acggcaccac gcgcgcagag ggatacgccc gcagcagctg ggcctggcgc cggaggctcg
     3661 aggtgcccac ggtgccctcc tcgtcgaagc ccgccgtcgc cgtcgccagc acgtcgtagg
     3721 ggaggctgcg cctgggaacg gccgccacga cgacgccctc ggggtcgtcc gagggcaggt
     3781 ccttcgccga gtgcactgcg aggtccacgt cgccggtcag aagcgcctcg gtgatctcgg
     3841 ccacgaacag ccccttcgtg ccctgagggg aggcctccgc cggggcgccc cgatcccccg
     3901 ccgtggtcat cgggaccagc tcggcctcgt gcccgcttcc ccccaggagc tcggcgacct
     3961 cgctcgcctg ggcgagcgcc agcctcgacc gacgcgtgcc gatccggatc cgcactagtc
     4021 cttcggaggc tccagcccga acagctcggc cagaacgcgc gcgtgtgcct cgccgacgcc
     4081 cgcgccccac cgctccttca gctcgaccac ggggtcgtgg agcagagtct tgacgatgcc
     4141 cttcgtcagg gtctccaccg cctcccgctc gcggtccgag aggcccgaca gccgtgaggc
     4201 cagccgctcc agctcggcgg cccggacctc ctcgccccgc tcgtggagcg cggcgatcag
     4261 cggggccagc ttcgccgccc ggcgctgggt cacgaacttc ctcgtctcct cggcgatcac
     4321 ccgtcccgcc gcctcgatct cgtctgcgat cccctcgcgc gaggcggcca ccacgtcgcg
     4381 gaggtcgtcg atgtcggcca cgcgcacgcc ggggacctcg cgggccgccg gatccacgtc
     4441 gcggggcacc gccaggtcga gcaggaacat ccgccgcccc ccggcggccc gctccacatc
     4501 ctcccggccc accacgacct gcgtggccgt ggtcgaggac acgacgaggt cggtccgggc
     4561 cagcgcatcc gagagctcct ccagcccccc gtgcgagcct cccacccgca ccgcgagccg
     4621 ctgcgccttc tccggggtcc ggttcaggac cgtcacctcc cccacaccct gccgccggag
     4681 ccagcccagc gcgagcgccg ccatctcccc cgccccgacc agcaggactg accgtccgcc
     4741 caggccgccc aggtgccgcg ccgcggcccc gccccccgcc tccacgaacg cgctcgggct
     4801 ggccccgatc ttcgtctcag ccctagccct gcgccccgcc cgtatcgcgt agcggaagag
     4861 cgccgagagc tgggggcccg ccgcctcctc gccccgggcc cgctggaagg cctgccgcac
     4921 ctgcgtcagg atctggggct cccccacgac catcgagtcg agcccggcgg ccacggagaa
     4981 caggtgctcg gccgtgtggt cctcatagtg ggagtagagg ggctccgcga actcctcggg
     5041 ctccacgccc ccggactccg acaggaaccg cttcaggtcc tggaaccccg tgtggtaccc
     5101 ctccaccgcc ccgtagacct cgacccggtt gcaggtcgag agcaggaccg cctcctcgac
     5161 cgacggcatc gaccgcacgt ggtcgtaggc cttggggaga tcctcgtcgc cccacgccag
     5221 ccgctcgagc acatccaccg atgcgcggcg gtacgagacg cccagtgcca ggatcggcat
     5281 gacggcctca gtctacgcgg tgggtcgctc ggcggtctcc ccgagcgact ggggcagggc
     5341 ctcgccccgc cgcacctcgt ccagcacggc cttccgctgc tcgtagaagg cgaggatctg
     5401 gagctcgacc gagaggtcca ccttccgaag ggacacccct tcggggcagg cgatcaccac
     5461 aggggagaag ttgaggatcg atcggatgcc cgcctcgacc atccgatccg ccacctcctg
     5521 cgccgcaggg cccggcgtgc agatcatccc gatctggatc ccgtgctcgc ggaccaggtc
     5581 gggcatctcc tcgggcgggc ggacctcgac cccggccacg cgctcgccca cccgagccgg
     5641 gtccacgagc cccgccacgc ggaagccccg ggcgtcgaac ccccggtagt tggccagggc
     5701 ctgccccagg ttgcccacgc cagcgatcag gacgggccaa tcctgggtca gcccgagctc
     5761 gcggcgcacc tggtggatca ggtaggcaac gtcgtacccc accccgcggg tgccgtagga
     5821 gccgaggtag gagaggtcct tccggacctt ggccgagttg acgccggccg cctcggccag
     5881 cgcctcggag gaaaccgtgg agcgcccgtc ctcggccatc tccaccagca cgcggaggta
     5941 aacggggaga cgtcccaccg tggcctcggg gatggggcgc ttcgtcatcc gtcgctcgtg
     6001 cgttcgtgaa cgcgcacgat acgatggtac cggcgggttc tcgatcgcac aaggtcagga
     6061 gagcaagcgg cgaagctcgt ccgcgtccac ccggtgctcg aaggcctcct cgccgtccac
     6121 ggtcacgacc gggaccctca gcccgtgctc ccgctccagc tcggggtcgc cctcgacgtc
     6181 cacctcgcgg tagtcgaagc ccagctcctc acggagcaag aggatcgtct cccgggcctc
     6241 gtcgcacagg tggcatccgg ggcgcgcgta catcaccacc tcggccacgg gcggcatcct
     6301 agggcctccc ccgcatctgc gatactcgct ccgtggccga ggccgacgac gagctcgccc
     6361 ggcgcgttgc cgaccgggac cccgaggcgt acgagcagat ctatcgcagg tacgcggcgg
     6421 cggcctacgg cgtcgccctg cgggtcctgc gcgaccccgc gctggcccag gaggtcgcgg
     6481 ccgagtcgtt cctgtcgctc tggtccacgc ccgaggcgta cgatccctcg cgggggaggt
     6541 tccgctcgtt cttcctttcg ctcgtgcacc atcgagccgt ggacgcggtt cgccgcgagg
     6601 agcgcctgcg ggagcgcgaa cggaaggtga accgggcgcc cctgccggac gaagactgga
     6661 gtgaggcggt ggtcgaggaa gcggacctgg tcgaccgccg tcgggaggtt cgggaggccc
     6721 tgtccgagct ccctcccgag cagcgcgagg cgctggagct gatgtacttc aggggctaca
     6781 cgcagtcgag gatcgcggag gagcgaggga tccccttggg aacggtgaag acgagggtgc
     6841 acgccgccat g
//
LOCUS       DASUTZ010000278         6664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_505031, whole genome shotgun sequence.
ACCESSION   DASUTZ010000278 DASUTZ010000000
VERSION     DASUTZ010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6664
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505031"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VF097_12050"
     CDS             <1..864
                     /locus_tag="VF097_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263568.1"
                     /translation="VAAGGPARLVVLVVWKGVGSDDLEASPAAWPTLRRLLREAAGTL
                     DARVGSLPLDPTAVLTTIGTGGLPREHGITGSTVRNEEGQAVPAWGRGAPYSVIAALG
                     DDLDEVLGLRPFVGLVGTSALDRGVIGKDWHLRTDEDDVVLEDRAVRQADAAADLLAG
                     GYGADDVPDLLAVVMHDRVPAMDRALQRIVQAVPPSTAIVVTSTGPLTTQEAMDADRI
                     RAEVEGASAAGLTEAVAVGGLFLDQDVLVEGDASQEDAIRALRGIRATSGGPMFADVF
                     GGRAVEFGRYC"
     gene            864..1874
                     /locus_tag="VF097_12055"
     CDS             864..1874
                     /locus_tag="VF097_12055"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEX6263569.1"
                     /translation="MTVIEEPRLRLPGERRPAWRGGLVLLLVLIATGTILRLLDAAGL
                     PPVRNFLLVWASLLVEAFPFILLGAIVSAAIEVFVPARLFARLARLPGPVQMPVAGLG
                     GFAFPVCECGSVPVARRLVVKGLTPAAAITFMLAAPILNPVVLVATAVAYRGRDVLWV
                     MVGGRAILGLVVALAVGWVVARKRKRQLLRAPEDHHEPGEPLARRWPAYFAHLSSDFV
                     MLGRYLVIGAAVASALQTFVPQGIIGTVAETPVLNVVAMMALAGILSLCSESDAFVAA
                     SFVQFGAGAQLAFLVFGPMVDAKLGFLYAGTFSRGFLRTVVAVVAAVTLAGALWVEVL
                     AG"
     gene            1871..2569
                     /locus_tag="VF097_12060"
     CDS             1871..2569
                     /locus_tag="VF097_12060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEX6263570.1"
                     /translation="MRRLSSTRVAVGLALAAWAGVFWFLLVTGRWALYLSSRVFWVVP
                     VGAVLLTAAALGRLLTARTERAEPLAVRDTWGLALVLLPVVAVLVLPPAALGSYAASR
                     RSASAGFVTPAGDPSSGEVTLVDVAGAMWSQTAADALTERAGSEVSFVGFVVRREGTP
                     ADEFVLTRFLVSCCVADALSVQVRVVGAAPGAFEEDEWVRVRGTLYPLPDGVVVDAAS
                     VKAVPRPSQPYLNV"
     gene            complement(2559..4172)
                     /locus_tag="VF097_12065"
     CDS             complement(2559..4172)
                     /locus_tag="VF097_12065"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6263571.1"
                     /translation="MLRAARVLAALLIVAAGCTGGLEDPVPDDAPTIPAMLRALGADP
                     VRQLARGYVPERSGDVVLVPAPGSTVAQWPGGLRSPADPRTTHGAPWDYIARVPIILR
                     GPGHVPAGAAPDRPVDVADIAPTIADLLAFEWEAPDARSLEEALLPDRDPLRVITLVV
                     HDGGGWNVLERWPDAWPFQRGLAAEGTIFVNATVGSAPPITAPIHANMGTGTYPRTHG
                     IAENTGRLPDGSLGELFFHEAWHLGMMSRGTRHPGGDQDVAVLWDHRETDDLFTNEEV
                     YRMPEYLRGREALEERVHELDLADGTADRRWKQADLDDIFFIPATPAFVEYQTDLVLE
                     MIDRERIGTDDVTDLLFVELKPSDNAGHLWNMESPMFEEVLRAQDRAVERIVGALDER
                     VGPDRHVVIVSADHGQSPRPDVAGGLRIDRFQVQEALNDAFGAEVVEAVHPDDLYLRW
                     DVLGEIGATAEDVARVAASLRFDQVAPEGTDLEALPEEVAAARPFAAAIPVRRLVELS
                     EEEIAALGAGAFPEADLTSPPDLARLLTR"
     gene            complement(4175..6025)
                     /locus_tag="VF097_12070"
     CDS             complement(4175..6025)
                     /locus_tag="VF097_12070"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6263572.1"
                     /translation="MHPRTLLVAAWAVPAAALVASAVAAFVAGWIAAAVVGVAVLVLG
                     AALVASLRPRPTDLGRRRVLAGLGIAGIGAVAGGSAVGRALSALEPEPAPGPVLRGMA
                     RRLGGPAMRSVLRGYHPQRSGDLQLILQPFNTSNYPHESRSLVPDDPRSSHSLVWGYT
                     DRVPIVVYAPSLVEPQDRTEEATLADLAPTTARLMGLDASFPDGEPLPGIEAPARPPK
                     VIVTFVIDGGGWNVLQKWPDAWPTLARLARDGALYRNGYMGSFPNVTASAHATIGTGA
                     FPRAHGISGHHVRAGRGTQPALGELGAADPSRILVPTLADFWAEEMGGRAWIGEIGYQ
                     VWHLGMIGRGGERPVAVYWDEVGNDWAVQNPDLYRMPAEVPSRAALTDRMRRYFGPER
                     GAERDREGGKAICCDPPIVEHQGDLLEAALRVEPIGESGVTDLLYINYKAPDYAGHVS
                     NMLDQHERVALQAVDRDLERLVTMLERRFRPGEWALIVTADHGQCPLIEDAGGVRLDL
                     VQLSEDLEGELDGGTVVGIRPSEVFLDADRAAVERLAASLAGYRYGENVGPYVPEEAV
                     LEERMDRRHFAAVLPGSFIGGLDEAEVAAFGSGRFPQADPGIPPRSMVEG"
     gene            6116..>6664
                     /locus_tag="VF097_12075"
     CDS             6116..>6664
                     /locus_tag="VF097_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012810694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6263573.1"
                     /translation="MRILILGGDGYLGWPTALHFSQRGHEVHTVDSYLRRRAHAEAGT
                     DSLTPILGSLPERARAWEEVTGKHIGVTEGDVTDWETLERVVLTFRPEAIVHYGEMPS
                     APYSMRDRAHAVFTQYNNVIGTLNVLFAMRDITPDAHLVKLGTMGEYGTPDIDIEEGF
                     FEVHHKGRSDTVPFPKQPGSLYH"
BASE COUNT          851 a         2437 c         2398 g          978 t
ORIGIN      
        1 gtggccgccg gcggtcccgc ccgcctggtc gtcctcgtgg tctggaaggg cgtcggctcc
       61 gacgacctcg aggcgagtcc ggccgcgtgg cccacgctcc ggcgcctcct tcgggaggct
      121 gcgggcacgc tggacgcgcg cgtcggatcg ctccccctcg accccacggc ggtcctcacc
      181 acgatcggca ccggaggact cccccgcgag cacggcatca ccggcagcac cgtacggaac
      241 gaggagggcc aggccgtccc cgcctgggga cggggcgccc cgtactcggt gatcgctgcc
      301 ctcggggacg acctcgacga ggtcctgggg ctgcgtccgt tcgtgggcct ggtcggcacg
      361 agcgctcttg accggggcgt gatcggaaag gactggcatc tgcgaaccga tgaggacgac
      421 gtcgtgctag aggatcgggc ggtccgccag gccgacgcgg ctgcggacct cctggccggg
      481 ggatacggcg ccgatgacgt gcccgacctg ctcgccgtgg tgatgcacga ccgcgtgccg
      541 gcgatggatc gggctctaca gagaatcgtc caggccgtcc ctccctcgac cgcgatcgtg
      601 gtcacctcca ccggaccgct gacgacccag gaggcgatgg acgccgaccg aatccgggcc
      661 gaagtggagg gcgccagtgc cgccggcctg accgaggccg tggcggtggg cgggctcttc
      721 ctcgatcagg acgtgctcgt ggagggggac gccagccagg aggacgcgat ccgggccctg
      781 cgcgggatcc gggcgacgag cggcggcccg atgttcgccg acgtcttcgg gggccgcgcc
      841 gttgagttcg ggaggtactg ctgatgaccg tgatcgagga gcctcgcctc cgcctccccg
      901 gcgagcgacg tcccgcgtgg cgcgggggcc tcgtgctcct cctcgtcctc atcgcgaccg
      961 gaacgatctt gcggctcctc gacgccgccg gcctccctcc ggtcaggaat ttcctcctcg
     1021 tctgggcctc gctcctcgtg gaggccttcc ccttcatcct gctgggagcg atcgtctccg
     1081 ccgcgattga ggtcttcgtg ccggcgcggc tcttcgcccg cctggcgcgc ctgccgggcc
     1141 cggtccagat gccggtggcc ggcttggggg gcttcgcctt ccccgtctgc gagtgcgggt
     1201 cggtccccgt ggcccggcgg ctcgtggtca agggcctgac gccggcggcc gcgatcacct
     1261 tcatgctggc cgcgccgatc ctcaaccccg tcgtgctcgt ggccacggcc gtcgcctacc
     1321 gaggtcgtga cgtcctgtgg gtgatggtcg ggggccgggc gatcctgggc ctcgtcgtgg
     1381 ccctcgcggt cggatgggtc gtggcccgga agcggaagcg gcagctgctg cgcgcccccg
     1441 aggaccacca cgagcccggg gaaccgctcg cccggcggtg gccggcctac ttcgcccatc
     1501 tttcctccga cttcgtcatg ctcggtcgct acctcgtgat cggggccgcg gtggcttcgg
     1561 ccctccagac gttcgtgccc caggggatca tcgggacggt ggccgagacc cccgtcctga
     1621 acgtcgtggc catgatggcc ttggccggga tcctctcgct ctgctccgag tcggacgcct
     1681 tcgtcgccgc gtcgttcgtg cagttcggcg cgggggcgca gctggccttc ctggtgttcg
     1741 ggccgatggt cgacgcgaag ctcgggttcc tctacgcggg gacgttcagc cgcgggttcc
     1801 tgcggacggt cgtggcggtg gtggccgccg tgacgctggc cggcgccctc tgggtggagg
     1861 tgctggccgg atgaggcggc tgagctcgac ccgggtcgcc gtcggcctcg cgctggccgc
     1921 gtgggcgggg gtcttctggt tcctcctcgt caccggccgc tgggcgctct acctctcatc
     1981 tcgcgtcttc tgggtcgtgc ccgtgggagc ggtcctcctc accgccgccg ccctgggacg
     2041 tctcctcacc gcgaggaccg agcgcgcgga gccgctggcc gtccgcgaca cgtgggggct
     2101 cgcgctcgtg ctcctgccgg tcgtggcggt gctggttctc ccgcccgcag cgctcggttc
     2161 ctacgccgcc tcccgccgtt cggcgagcgc gggcttcgtc acgccggcgg gcgacccctc
     2221 gtctggtgag gtgacgctcg tggacgtggc cggcgcgatg tggtcccaga cggcggcgga
     2281 cgccctgacc gagcgcgccg gcagcgaggt ctcgttcgtg ggattcgtcg taaggcggga
     2341 gggaacgccc gccgacgagt tcgtgctgac ccggttcctc gtctcgtgct gcgtggccga
     2401 cgcgctcagc gtccaggtcc gggtggtggg cgctgccccc ggggcgttcg aggaggacga
     2461 gtgggtgcgg gtccggggaa cgctctatcc actaccggac ggagtcgtcg tggacgccgc
     2521 ctcggtgaag gctgtccctc gcccgtccca gccctacctc aacgtgtaag gagccgggcc
     2581 aggtccggcg gcgacgtcag gtcggcctcc gggaaggccc ccgcccccag cgcggcgatc
     2641 tcctcctcgc tcagctcgac gagccgccgg accgggatcg cggcggcgaa gggtcgggcg
     2701 gccgccacct cctcggggag ggcctccagg tcggtgccct cgggggccac ctggtcgaag
     2761 cggagcgaag ccgccacccg ggccacgtcc tcggccgtgg ccccgatctc accgaggacg
     2821 tcccagcgga ggtacaggtc gtccgggtgc acagcctcca ccacctcggc tccgaaggcg
     2881 tcgttgagcg cctcctgcac ctggaagcga tcgatccgga ggccgccggc gacgtctgga
     2941 cggggcgact gcccgtggtc ggcgctcacg atcacgacgt gccggtccgg accgacccgc
     3001 tcgtcgagcg ctcccacgat gcgctccacc gcgcggtcct gcgcccggag cacctcctcg
     3061 aacatcgggg actccatgtt ccacaggtgg cccgcgttgt cgctcggctt cagctccacg
     3121 aacaggaggt cggtcacgtc gtcggtcccg atcctctccc ggtcgatcat ctccagaacg
     3181 aggtcggtct ggtactcgac gaaggcgggg gtggcgggga tgaagaagat gtcgtcgagg
     3241 tcggcctgct tccatcggcg gtcggcggtc ccgtccgcga ggtcgagctc gtggacccgt
     3301 tcctcgagcg cctcccggcc ccggaggtac tcgggcatcc tgtagacctc ctcgttcgtg
     3361 aagaggtcgt cggtctcgcg gtggtcccac agcacggcaa cgtcctgatc gcctccgggg
     3421 tgacgcgtgc cgcggctcat catccccagg tgccacgcct cgtggaagaa gagctctccg
     3481 agcgaaccgt cggggagacg ccccgtgttc tcggcgatcc cgtgggtccg cgggtaggtc
     3541 ccggtcccca tgttggcgtg gatcggcgcg gtgatcggcg gcgccgaccc caccgtcgcg
     3601 ttcacgaaga tggtcccctc ggcggcgagg ccgcgctgga agggccaggc gtccggccac
     3661 cgctccagga cgttccaccc gccgccgtcg tgcacgacga gcgtgatcac cctgagcgga
     3721 tcgcggtccg ggagcagggc ctcctccagc gagcgtgcgt cgggtgcctc ccactcgaac
     3781 gcgagcaggt ccgcgatcgt tggagcgatg tcggctacgt ccaccggccg atccggcgcg
     3841 gcgcctgccg gcacgtggcc cgggccgcgg aggatgatcg gcacgcgggc gatgtagtcc
     3901 cagggcgccc cgtgcgtcgt ccgagggtcg gcggggctgc ggaggccgcc cggccactgg
     3961 gccacggtgc tgcccggcgc cggcaccagg acgacgtcgc ccgagcgctc ggggacgtac
     4021 ccacgggcca gctgacggac ggggtcggca cccagcgccc ggagcatcgc ggggatcgtc
     4081 ggcgcgtcgt cggggacggg gtcctcgagc cctcccgtgc atcccgcggc cacgatcagg
     4141 agggcggcca ggacccgggc ggctcgaagc acggtcagcc ttccaccatg cttcggggtg
     4201 gaatccccgg atcggcctgc gggaaccgac cactgccgaa cgcggcgacc tctgcctcgt
     4261 cgaggccccc gatgaacgac cccgggagca ccgcggcgaa gtggcgccgg tccatccgct
     4321 cctcgagcac ggcctcctcg gggacgtacg gccctacgtt ctcgccgtag cggtagccgg
     4381 cgagggaggc cgccagccgc tcgacggcgg cgcggtcggc gtcgaggaag acctcggacg
     4441 ggcggatgcc gacgaccgtg ccgccgtcga gctccccctc caggtcctcc gacagctgca
     4501 cgagatcgag acggacgccc cccgcatcct cgatcagcgg acactgcccg tgatcggccg
     4561 tgacgatgag cgcccactcc cccggacgaa accgccgctc gagcatggtc acgagcctct
     4621 ccaggtcgcg atccaccgcc tggagggcga cgcgctcgtg ctgatcgagc atgttcgaga
     4681 cgtgccccgc atagtcggga gccttgtagt tgatgtagag gaggtccgtg acgccgctct
     4741 ccccgatcgg ctccacgcgc agcgcggcct cgagcaggtc gccctgatgc tcgacgatcg
     4801 gcgggtcgca gcagatcgcc ttcccgccct cgcgatcgcg ctccgccccg cgctcggggc
     4861 cgaagtagcg gcgcatccga tccgtaaggg ccgcgcggga cggcacctcg gcgggcatgc
     4921 ggtacaggtc gggattctgg acggcccagt cgttccccac ctcgtcccag tacacggcca
     4981 cgggccgctc ccctcctcgc ccgatcatcc cgaggtgcca cacctggtag ccgatctcgc
     5041 cgatccaggc ccggccgccc atctcctcgg cccagaagtc ggccaaggtg gggacgagga
     5101 tccgcgacgg gtccgcggcg cccagctccc cgagcgccgg ctgggtccct cgcccggccc
     5161 gcacgtggtg cccggagatc ccgtgggctc gagggaacgc cccggtcccg atcgtggcgt
     5221 gggccgaggc ggtgacgttg ggaaacgagc ccatgtagcc gttccggtac agggcgccgt
     5281 cccgggccag ccgagccagc gtgggccacg cgtccggcca cttctggagg acgttccatc
     5341 ccccaccgtc gatcacgaag gtcacgatca ccttgggcgg ccgcgcgggc gcctcgatgc
     5401 ccggcagcgg ctcgccgtcg gggaacgacg catcgaggcc catcaggcgc gccgtcgtcg
     5461 gggccaggtc ggccagcgtc gcctcctcgg tacggtcctg gggctcgacc aagctgggcg
     5521 cgtacacgac gatcgggaca cgatcggtgt agccccagac cagcgaatgg ctgcttcgcg
     5581 ggtcgtcggg cacgaggctc ctcgactcgt gcgggtagtt cgaggtgttg aacggctgga
     5641 ggatgagctg gaggtccccc gaccgctggg gatggtagcc gcggagcacg ctccgcattg
     5701 cggggccgcc caggcggcgc gccatgcctc gcaggacggg ccccggcgcg ggctcgggct
     5761 ccagcgcgct gagggctctc ccgacggccg agcctcccgc caccgcgccg atccccgcga
     5821 tcccgaggcc ggcgagcacc cggcggcggc ccagatccgt gggacgcgga cggagcgagg
     5881 ccacgagggc cgcccccagg accagcaccg cgacgccgac cacggcggcg gcgatccagc
     5941 cggcgacgaa cgcggccacc gcggaggcca cgagggctgc cgcgggcacg gcccaagcgg
     6001 ccacgaggag ggttcggggg tgcacgagcc gattgtcgca gaacaacccg gacacgtccc
     6061 gggcccgggc ccgatgacta caatgagacg ttccccactg ttggaggcac gcggcatgcg
     6121 catcctgatc ctcggcggag acggctacct gggctggccc accgccttgc acttctccca
     6181 gcgcgggcac gaggtccaca ccgtggacag ctacctgcgc cggcgggccc acgccgaggc
     6241 ggggaccgac tcgctgacgc cgatcctggg ctcgctcccg gagcgggccc gggcctggga
     6301 ggaggtgacc gggaagcaca tcggcgtgac cgagggggac gtcaccgact gggagaccct
     6361 ggagcgcgtc gtcctcacct tccgccccga ggcgatcgtg cactacggcg agatgccctc
     6421 ggccccctac tcgatgcgcg accgcgccca cgccgtcttc acgcagtaca acaacgtgat
     6481 cgggaccctg aacgtgctgt tcgcgatgcg ggacatcacc cctgacgccc acctcgtcaa
     6541 gctcggaacc atgggcgagt acggcacgcc ggacatcgac atcgaggagg gcttcttcga
     6601 ggtccaccac aagggacgga gcgacaccgt gccgttcccc aagcagcccg ggtcgctcta
     6661 ccac
//
LOCUS       DASUTZ010000279         4983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1153427, whole genome shotgun sequence.
ACCESSION   DASUTZ010000279 DASUTZ010000000
VERSION     DASUTZ010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153427"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VF097_12080"
     CDS             <1..237
                     /locus_tag="VF097_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263574.1"
                     /translation="AQDPTVAEVAERLEQETTRLNESITSLEQSIAAEEPAGEEEDPA
                     AEARTLAMIALIVGGVALVAGLVVGGMALRRAGA"
     gene            242..2197
                     /locus_tag="VF097_12085"
     CDS             242..2197
                     /locus_tag="VF097_12085"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEX6263575.1"
                     /translation="MGRALLLAGMIALGIVLTAPGASGHALLESSSPGDGELLGTAPE
                     EILLSFSEPLDLGLSSVSVVDSQGAEVDAGELERLGTEREVRLALPQDLSDGVYSVSW
                     RVLSRVDGHTTAGAFTFGVGVAASEVTPSGDSTQVEYPSPTPLSVGGRWGFYWGLVLL
                     LGAAMGGLFLFPGRVRWAVWLLAIGWTVGALGLVAMVVAEASAADIGLGSLLGSDRGT
                     ILLVRAEALVAAGIVGAAALARPGRVAWGALGATTLAAMLVHAYAGHAGAGRNAVLAV
                     GVQWIHIVAVGVWVGGLAWLLVGLVGADPETRAPAAARFSSIATWAIVFVALTGFLRA
                     WSEVGSLGDLFNTGFGLAAVGKSALFGGMLALGAYNRFRLIPRGRLPTLRRTMSGELA
                     MAVGVFGLTGLMAGLVPPSQLQEEEEVAAAPERVVVEGSDFATTVRMRLTATPGTPGP
                     NAFQARISDYDTGEPVEATAVSLRFSMPDRGDVGTTELELDEVEPGLWRGEGTNVSVA
                     GTWRIGVLIQQPADSVRLELGLETKGAEPEIEVIDGGPGQPDIYVIPLGEGRTVQAYV
                     DPGAAGSNEVHYTFFTPEGGELPMEDITIRAALEGGDPIELEPRRLTEGHFVASAELE
                     AGAWSFTAEATAQDGTVLTATFEEEIE"
     gene            2194..2634
                     /locus_tag="VF097_12090"
     CDS             2194..2634
                     /locus_tag="VF097_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263576.1"
                     /translation="MRMRLAALAAAMVLAACTGSGTEETPTPDAAETTAERPSSPAEL
                     TIVEPQPGAVYPPDDVPVRLELENAEILEEVSTDLRGDQGHIHLVLDGETVTLLGGLD
                     ENLAELAEGGQLEPGMHILEAEFVAADHGFFLPRVITTVTFQVE"
     gene            2631..3170
                     /locus_tag="VF097_12095"
     CDS             2631..3170
                     /locus_tag="VF097_12095"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX6263577.1"
                     /translation="MIAEPRSSTRAPRGAAFVAGAGLAALALRSPGVEAVVVVAALGV
                     AGLALPVPARDRPARAPTWVAAVAIGALAMALVARAATVPLAFAPLPVGVSVLASVAE
                     EAFFRRYLYGWLAARGPAVAILVSAAAFALVHLPLYGPDALALDLAAGLLLGWQRWAT
                     GTWTAPAATHALANVLAYA"
     gene            3182..4417
                     /locus_tag="VF097_12100"
     CDS             3182..4417
                     /locus_tag="VF097_12100"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6263578.1"
                     /translation="MHRIVALLLLAAACAPASGSDPLVVGAVYPTAGSQGIGGIEEFR
                     GVRLAVERINAEGGVNGRLVRLSVQPAESAEGAPLAVRAAAADGAEVILGSYGSTIST
                     KAAKVASDLDLVFWETGAVGEIEDGVRAGERFFRLVSMGGSLGDEGVRFLDEQLLPMM
                     GEDPRTLDWGVLYVDDVYGRSVGLGAAETIEEAGLPLAGTFPYDLATLDGERLVRRIG
                     RAGVDVLVVSAYLEDGVELRKEMVRQGLPLVASVGTSSSYCMREFAAALGEDAVGLFA
                     SDKPNGEVMEAADLRPDAAGALEWANREYERRWDEPMPVPALSGFSGAWALFRHVLPG
                     TGSATAEEVAEAALAADLPLGSLPNGSGLRFAPGGDPRAGVNLRATSVIWEWVDEQER
                     EVVWPPDLATSPIVPIPIA"
     gene            4414..>4983
                     /locus_tag="VF097_12105"
     CDS             4414..>4983
                     /locus_tag="VF097_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263579.1"
                     /translation="MMRLRWGLAAAGAYLLVAAALLAGGRPVLPLFDGLAPVEYRWVT
                     PPPGFEQGNQQPLDGRSRAPLEEGGAITVITADGQAQISILTEDVVVPEGQRAVVGTL
                     TPVDPAGLGPPPTGLRFDSNAYEAEVVYVPSGEPLEFAEGDPAEDPPSATILLRYAAH
                     ATQLARWDEATSSWTNLEATLLRGTLQIYG"
BASE COUNT          593 a         1762 c         1928 g          700 t
ORIGIN      
        1 gcccaggacc cgaccgtggc cgaggtcgcc gagcgcctgg agcaggagac gacgaggctg
       61 aacgagagca tcacctcgct cgagcagtcg atcgccgcgg aggagccggc cggagaggag
      121 gaggaccctg cggcggaggc gcgcacgctg gcgatgatcg cgctgatcgt cgggggcgtg
      181 gcgctcgtcg cgggcctggt cgtcggcggc atggcgctcc ggagggccgg ggcctagacg
      241 gatggggaga gcgctcctgc tcgccgggat gatcgccctg gggatcgtgc tcacggctcc
      301 cggcgcgagc ggccatgcgc tgctcgagtc gtcgtcgcct ggggacgggg agctgctcgg
      361 gacggcccct gaggagatcc tcctctcctt cagcgagccg ctggacctcg ggctctcctc
      421 ggtctcggtg gtggactccc agggcgccga ggtcgacgcg ggcgagctgg agcgcctggg
      481 taccgagcgg gaggtccgcc tggccctccc gcaggatctt tcggacggcg tgtactccgt
      541 ctcgtggcgc gtgctctccc gcgtggacgg acacacgacg gcgggagcgt tcacgttcgg
      601 cgtgggcgtg gccgcctcgg aggtgacgcc gtcgggagac tcgacgcagg tcgagtaccc
      661 gagcccgacg ccgctctcgg tgggcgggcg ctggggcttc tactgggggc tcgtcctcct
      721 gctcggcgcc gccatggggg gcctcttcct gttccccggc cgcgtccgtt gggcggtgtg
      781 gctgctcgcg atcggctgga ccgtgggagc gctcgggctc gtggccatgg tggtcgccga
      841 ggcaagcgcc gccgacatcg gcctcggctc gctcctcggg tcggaccgag ggacgatcct
      901 gctggtccga gccgaggccc tcgtcgctgc ggggatcgtg ggggcggcgg cgctggcgag
      961 acccggccgc gtcgcgtggg gggcgctcgg cgccacgacg ctggccgcga tgctcgtgca
     1021 cgcctatgcg gggcacgccg gggcggggag gaacgcggtt ctggccgtcg gcgtgcagtg
     1081 gatccacatc gtggccgtgg gcgtctgggt gggcggcctg gcctggctgc ttgtggggct
     1141 cgtgggggcc gacccggaga cccgggcccc ggcggccgct cgcttctcca gcatcgcgac
     1201 gtgggcgatc gtgttcgtcg ctctcacggg cttcctgcgc gcctggagcg aggtgggctc
     1261 gctcggcgac ctcttcaaca ccggcttcgg gctcgcggcc gtggggaaga gcgccctctt
     1321 cgggggcatg ctggcgctag gcgcctacaa ccgcttccgc ctcatccccc gagggcggct
     1381 gcccacgctc cgccggacga tgagcggcga gctcgccatg gccgtcggcg tgttcgggct
     1441 caccgggctg atggccgggc tcgtgccccc ctcccagctg caggaggaag aggaggtcgc
     1501 cgccgcgccg gagcgggtgg tcgtggaggg gtccgacttc gccacgacgg tccggatgcg
     1561 gctcaccgcc acgccgggca cgcccggccc caacgcgttc caggcccgga tctccgacta
     1621 cgacaccggc gagccggtgg aggccaccgc cgtctcgctc cggttctcga tgcccgaccg
     1681 cggcgacgtc gggaccaccg agctcgagct ggacgaggtg gagcccggcc tgtggcgggg
     1741 ggagggcacg aacgtctcgg tcgccgggac gtggcgcatc ggcgtgctga tccagcagcc
     1801 ggccgactcc gtccgcctgg agctcgggct ggagacgaag ggggccgagc ccgagatcga
     1861 ggtgatcgat ggcggccccg gacagcccga catctacgtg atcccgctcg gcgagggacg
     1921 caccgtccag gcctacgtgg acccgggcgc cgccggctcc aacgaggtcc actacacgtt
     1981 cttcacgccc gagggcggcg agctgccgat ggaggacatc acgatccggg ccgcgctcga
     2041 gggcggagac ccgatcgagc tcgagccccg gcgcctgacc gaagggcact tcgtagcgtc
     2101 ggcggagctc gaggcgggag cctggtcgtt caccgccgag gcgacggccc aagacggtac
     2161 cgtgttgacc gcgaccttcg aggaggagat cgagtgagga tgagactcgc ggcgctggcc
     2221 gccgccatgg tgctcgccgc ctgcacgggc agcgggaccg aggagacgcc gaccccggac
     2281 gcggccgaga cgacggcgga gcggcccagc tctcccgccg agctgacgat cgtggaaccc
     2341 cagcccggcg ccgtgtatcc gcccgacgac gtgccggtgc ggctcgagct ggagaacgcc
     2401 gagatcctcg aggaggtctc cacggacctc cgcggcgacc aggggcacat ccacctcgtg
     2461 ctggacgggg agaccgtgac gctcctgggc ggcctggacg agaacctggc cgagctcgcg
     2521 gaggggggtc agctggagcc cgggatgcac atcctggagg cggagttcgt cgccgccgac
     2581 cacgggttct tcctcccgcg cgtcatcacg acggtaacct tccaggtcga gtgatcgctg
     2641 aaccccgctc ctcgacccgc gcgcccaggg gggctgcgtt cgtggccggc gccggcctcg
     2701 cggcactcgc cctgcggtcg ccgggcgtgg aggcggtcgt cgtggtcgcc gccctgggcg
     2761 tggcggggct cgcgctcccc gtcccggccc gggaccggcc cgctcgagcc ccgacctggg
     2821 tggccgcggt cgcgatcggg gcgctggcca tggcgctggt ggctcgagcc gccaccgtgc
     2881 ccctggcctt cgcccccctg ccggtcgggg tttccgtcct cgcttcggtg gccgaggagg
     2941 cattcttccg caggtacctg tacggatggc tggcggcccg cgggcccgcg gtggccatcc
     3001 tcgtctcggc ggcggcgttc gcgctcgtcc acctgccgct gtacgggccc gacgcgctgg
     3061 cgctcgacct ggccgccggc ctgctgctcg gctggcagcg gtgggcgacc gggacctgga
     3121 ccgcgccggc cgcgacccac gccctcgcca acgtgctcgc ctacgcatga ggaggatgaa
     3181 gatgcaccga atcgtcgccc tgctgctgct cgcggcggcg tgcgccccgg cctcggggtc
     3241 ggacccgctc gtggtgggcg ccgtctaccc cacggccggc agtcagggga tcggggggat
     3301 cgaggagttc cgcggggtcc ggctggcggt ggagcggatc aacgccgagg ggggcgtgaa
     3361 cggccgtctc gtgcggctca gcgtccagcc ggccgagagc gccgaggggg cgccgctcgc
     3421 ggtgcgcgcc gcggcggccg acggggccga ggtgatcctg ggcagctacg gcagcacgat
     3481 ctcgacgaag gcggccaagg tggcgtcgga cctcgacctc gtcttctggg agaccggggc
     3541 cgtgggcgag atcgaggacg gcgtccgcgc cggggagcgg ttcttccgac tcgtctcgat
     3601 gggcggctcg ctcggggacg agggggtgcg cttcctcgat gagcagctgc tcccgatgat
     3661 gggcgaggat ccccgcacgc tcgactgggg cgtcctctac gtcgacgacg tctacggtcg
     3721 ctccgtgggc ctcggcgccg ccgagacgat cgaggaggcc gggctgccgc tggccggcac
     3781 cttcccctac gacctcgcca cgctcgacgg cgagcgcctc gtccggagga tcggccgggc
     3841 cggcgtggac gtgctcgtcg tctccgccta cctcgaagac ggcgtggagc tccgcaagga
     3901 gatggtgcgg cagggcctcc cgctcgtggc gagcgtgggg acctcctcct cgtactgcat
     3961 gcgggagttc gcggcggcgc tgggcgagga cgccgtgggg ctcttcgcct ccgacaagcc
     4021 gaacggcgag gtgatggagg ccgccgacct ccgaccggac gcggccgggg ccctcgagtg
     4081 ggccaaccgc gagtacgagc gtcgctggga cgagccgatg ccggtgcccg ccctctccgg
     4141 gttctcggga gcgtgggcgc tgttccgtca cgtccttccc gggaccggct cggccacggc
     4201 cgaggaggtc gcggaggccg cgctggccgc ggacctcccg ctgggctcgc tgcccaacgg
     4261 cagcggcctc cggttcgccc cggggggcga cccgcgcgcc ggcgtcaacc tccgggccac
     4321 gagcgtgatc tgggagtggg tcgacgagca ggagcgcgag gtggtctggc ccccggacct
     4381 ggccacgagc cccatcgtgc ccatcccgat cgcatgatga ggcttcgctg gggcctcgcc
     4441 gctgccgggg cgtacctgct ggtggccgcc gccctcctcg cggggggacg gccggtgctg
     4501 ccgctcttcg acggcctcgc cccggtggag taccgatggg tgacccctcc gcccgggttc
     4561 gagcagggca accagcagcc cctcgacggc cgttcccgtg ccccgctcga ggagggagga
     4621 gcgatcacgg tcatcaccgc cgacggccaa gcgcagatct cgatcctcac cgaggatgta
     4681 gtcgtccccg agggtcagcg cgcggtggtg gggacgctga cgcccgtgga tccggccggc
     4741 ctcgggccgc cgccgaccgg gctccggttc gactccaacg cgtacgaggc cgaggtcgtc
     4801 tacgtgccgt cgggggagcc gctggagttc gcggagggtg accccgccga ggacccgccc
     4861 agcgccacga tcctgctccg ctacgcggcc cacgccaccc agctggcgcg gtgggacgaa
     4921 gccacgagct cgtggaccaa cctggaggcc accctgctgc gaggaaccct ccagatctac
     4981 ggg
//
LOCUS       DASUTZ010000280         2207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1543898, whole genome shotgun sequence.
ACCESSION   DASUTZ010000280 DASUTZ010000000
VERSION     DASUTZ010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543898"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..836)
                     /gene="hisF"
                     /locus_tag="VF097_12110"
     CDS             complement(75..836)
                     /gene="hisF"
                     /locus_tag="VF097_12110"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX6263580.1"
                     /translation="MTLARRLMPCLDVEAGRVVKGTRFVDLRDAGDPAELAARYDAEG
                     ADELVFLDITASVEGRGAMLDVVARAADQIFIPLTVGGGVRTEEDVNALLRAGADKVG
                     LNTHGVREPELLARCADRFGTQCMVIAIDAKRRNGGWEVVVEGGRTPTGLDAVAWAAR
                     ATADLGAGEVLLTSIDRDGTGDGYDLELLRAVADAVEVPVVASGGAGELVHFARAFTE
                     GHADAVLAASRFHFGEFTVPQVKEYLAARGIPVRG"
     gene            complement(833..1537)
                     /gene="hisA"
                     /locus_tag="VF097_12115"
     CDS             complement(833..1537)
                     /gene="hisA"
                     /locus_tag="VF097_12115"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEX6263581.1"
                     /translation="MIVIPAIDVREGKVVRLRHGRLQEMIVYADDPVETARGYAADGA
                     RQVHVVDLDAAFGVGDNRAVIAEVAREAGVPIQVGGGLRSLLLIEGVLAVGASRVVLG
                     TEAILDPAFLAECVERYGQRVVVALDTDGDVVKVRGWTEPAGSFDEILERLVRARVPR
                     LLVTAIARDGTLEGPDLDLYRRLSPLGVPVLASGGVSSLEDLRRLARAGVEGAVVGKA
                     LYEGRFTLREAIEVPV"
     gene            complement(1534..2130)
                     /gene="hisH"
                     /locus_tag="VF097_12120"
     CDS             complement(1534..2130)
                     /gene="hisH"
                     /locus_tag="VF097_12120"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX6263582.1"
                     /translation="MAILDYGFGNLRSVQRAVERVGGAGLVTSDPDEARAADAFVVPG
                     VGAFAACVAGLRNAGLDGLVREIVDSGRPVLGVCLGMQVLFDWGEEGPADGLGILRGK
                     VRRLPQGLTVPHMGWNEVRWSAAHPLTEGIPDGTRFYFVHSYVCDPEEEVTVGVTDYG
                     GPFAAAVGRENVFATQFHPEKSGEPGLRIYANLVEAAA"
BASE COUNT          323 a          864 c          740 g          280 t
ORIGIN      
        1 gtcctgcacg accgccgcga taaggccccg ctcgtcgtac gcgaggtcgg cgatcgagtc
       61 cacggctcgc tcggtcatcc gcgcacgggg atccctcggg cagcgaggta ctccttcacc
      121 tggggcacgg tgaactcgcc gaagtggaag cgggacgcgg ccaggacggc gtcggcgtga
      181 ccctcggtga aggcccgagc gaagtgcacc agctcgccgg ccccgccgct ggccaccacc
      241 gggacctcga ccgcgtcggc cacggcccga agcagttcca ggtcgtagcc gtccccggtg
      301 ccgtcgcggt cgatcgacgt gaggagaacc tcgccggcgc ccagatcggc cgtggcccgc
      361 gcggcccacg ccacggcgtc gaggccggtg ggcgtgcggc cgccctcgac gacgacctcc
      421 cagccgccgt tccggcgctt cgcgtcgatc gcgatcacca tgcactgcgt cccgaagcgg
      481 tctgcgcagc gggccagcag ctccggctcg cggaccccgt gggtgttgag cccgaccttg
      541 tcggccccgg cccggagcag ggcgttgacg tcctcctcgg tgcggacccc gccgcccacc
      601 gtgaggggga tgaagatctg atcggccgct cgggccacga cgtcgagcat cgccccgcgg
      661 ccctcgaccg aggccgtgat gtcgaggaac acgagctcgt ccgcgccctc cgcgtcgtag
      721 cgggccgcga gctccgcggg gtcgcccgca tcgcggaggt cgacgaagcg ggtgcccttc
      781 accacgcggc ccgcctccac gtccaggcag ggcatcagcc tccgagcgag ggtcacaccg
      841 gcacctcgat cgcctccctc aacgtgaagc gcccttcgta cagggccttc cccaccaccg
      901 caccctcgac tccggctcga gccaggcgcc gcagatcctc cagggagctc acaccgcccg
      961 aggccaggac ggggaccccc agagggctta acctccggta gaggtctaga tcgggaccct
     1021 ccagggtccc gtcgcgggcg atcgccgtga ccagcaggcg ggggacccgg gcacggacca
     1081 ggcgctccag gatctcgtcg aaggaccccg cgggctccgt ccagccccgg accttgacca
     1141 cgtccccatc ggtgtcgagg gcaacgacca cgcgctgccc gtaccgctcc acgcactccg
     1201 ccaggaacgc cgggtccagg atggcctccg tgcccagcac gaccctggac gcccccaccg
     1261 ccaggacccc ctcgatcagc aagaggctgc gcaggccgcc tccgacctgg atggggacgc
     1321 ccgcctcccg cgccacttcg gcgatcaccg cgcggttgtc gccgacgccg aacgcggcat
     1381 cgaggtccac gacgtggacc tgccgcgcac cgtccgccgc gtagccgcga gcggtctcca
     1441 cggggtcgtc ggcgtacacg atcatctcct ggaggcggcc gtgtcggagg cgcacgacct
     1501 tcccctcccg cacgtcgatc gcggggatga cgatcacgca gccgcctcga cgaggttcgc
     1561 gtagatccgt aggccgggct cgccggactt ctcggggtgg aactgcgtgg cgaacacgtt
     1621 ctctcggccc acggccgcgg cgaagggccc cccgtagtcc gtgaccccca ccgtgacctc
     1681 ctcctcgggg tcgcagacgt aggagtggac gaagtagaag cgggtgccgt ccgggatgcc
     1741 ctcggtaagc ggatgcgctg cgctccagcg cacctcgttc cagcccatgt ggggcacagt
     1801 cagcccttgg ggaaggcggc ggaccttgcc cctgaggatc ccgagcccgt cggcgggtcc
     1861 ctcctcgccc caatcgaaga ggacctgcat cccgaggcac acgcccagca ccgggcgccc
     1921 cgagtcgacg atctcgcgca ccagaccgtc cagtcccgcg ttccggagcc ctgccacgca
     1981 ggcggcgaag gctcccacgc cggggacgac gaatgcgtcg gcggcgcggg cctcgtccgg
     2041 gtcggaggtc acaaggcccg cgccgccgac gcgctccacc gcgcgctgga cggacctcag
     2101 gttcccgaag ccgtagtcga ggatcgccac cgaggtggtc acagggaccc cttcgtggac
     2161 ggcgtccccc cgccgatctc ctcgcacgcg ctgcgcagcg ccaccgc
//
LOCUS       DASUTZ010000281         7653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_119181, whole genome shotgun sequence.
ACCESSION   DASUTZ010000281 DASUTZ010000000
VERSION     DASUTZ010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7653
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119181"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /gene="lepB"
                     /locus_tag="VF097_12125"
     CDS             <1..534
                     /gene="lepB"
                     /locus_tag="VF097_12125"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX6263583.1"
                     /translation="IKTFLVQAFYIPSESMVPTLRNGDRVLVNKLIFNIRDPDRGEIV
                     VFENPNLQEEDRNPFAAVWHWITEGLGFTVDPNKDFIKRVIAIPGDTIEVRGGRVFVN
                     GQRIREPYLVRNRDRSDFGPYSVPPDTYFMMGDNRANSQDSRSPAVGPVPREKIVGKA
                     FVILWPPSRFEWLGEGD"
     gene            513..1178
                     /locus_tag="VF097_12130"
     CDS             513..1178
                     /locus_tag="VF097_12130"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX6263584.1"
                     /translation="MARRGRLKNRYEQRLRERGFRLLAGVDEAGRGALAGPLVAAAVI
                     LPEDFDLDGVHDSKLLTPLQRVTVYPRILEAALAVAVCRAMPGRIDREGLHRTNLRLL
                     RRAIRTLDLEPEYVLTDGFPVGRMDVPCLAMKGGDALNGTVAAASIVAKVTRDRTMER
                     YHRRFPAYGFDTNRGYGTRRHWWALERYGPTPIHRLSFKGVGQASLFPRFEAHNGDEG
                     EVR"
     gene            1175..1480
                     /locus_tag="VF097_12135"
     CDS             1175..1480
                     /locus_tag="VF097_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003942715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2469 domain-containing protein"
                     /protein_id="HEX6263585.1"
                     /translation="MSGEELERYETEMELQLYREYRDVLPMFRYVVETDRRFYLANEV
                     EVTRHTESGEFLIEINLTDAWVWDMHRPARFVERVRVLTTQDVNIEEISKPEPPTGD"
     gene            1515..1865
                     /locus_tag="VF097_12140"
     CDS             1515..1865
                     /locus_tag="VF097_12140"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX6263586.1"
                     /translation="MRSRDRVWHEGERAAWGWYWERGYRLVARNWRSRVGELDLVVER
                     GGTLVFCEVKARASALAGPFDAVTPAKQRKVRAVAEAFLLATGTGPQACRFDVASVVL
                     GRRGARVHVFEDAF"
     gene            complement(1862..2674)
                     /locus_tag="VF097_12145"
     CDS             complement(1862..2674)
                     /locus_tag="VF097_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263587.1"
                     /translation="MSIAQGPSWGGNRMRNVLAGTAILELVLGVGFLIAGRSAGAGAT
                     GLTVTGAILAATGLLLLLWARSVGTSQRRAQQIVATGLPGTATIESFRQTGVALNEIN
                     PQVEMDLEVHVEGRDPYMVKKKEFIPGAMVGILTSGAALPVRVDPDDLSQVVVLWGAQ
                     DASAAQGTDPVELQRQIDERRAKVRENPLPATARFRKAKDTGVRVGDYRIMEIEVQIE
                     VDDGRPPHVDSGHAAVPSAVADRVRPGLELPAQIRRDDPNLFFVDWDQVPTL"
     gene            2747..3592
                     /locus_tag="VF097_12150"
     CDS             2747..3592
                     /locus_tag="VF097_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEX6263588.1"
                     /translation="MSYGATSATAPLKRVLVRPPMETAGWEELGWRSEPDLDRMVREH
                     EGYVAALEAAGVEVVLGTTRVPGNCDAIYACDVAIVTERGAIVLRPGKAGRRDEPEAV
                     AADLEAAGVPIIGRLEEPATAEGGDLLWLDERTLVVGRSYRTNDEGIAALRELLPEVE
                     VVSVDLPHHHGAGVILHLLSVISMLDEDLAVAHPPLLPVRLMEMLGERGVGLIEVPDE
                     ELDSQGPNVLALAPRVALALEGSPVTRRRMEAAGVDVRVYAGEDLCRKGDGGPTCLAQ
                     PILRG"
     gene            3627..5159
                     /locus_tag="VF097_12155"
     CDS             3627..5159
                     /locus_tag="VF097_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011056442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX6263589.1"
                     /translation="MYARAVGVAVVGVHGHPVSVEAHIGRGLPALQLTGLPGAAVQDA
                     RERVRPAVENSGLEWPLRRVTVNLSPADLRKEGPGFDLPIALAVLAATAQLDQERLPG
                     YASAGELSLKGSLVPTPGILAVAWAARESGLRGVIVPEANAAEAALVEGIEVVGAPSL
                     ARAVEFLRGRWEPGPPSEPPPGSVDGAAVDFSEVRGQAEARRALEIAAAGGHNVLLFG
                     PPGGGKTMLARRLPTILPAMSRDEALEVTRLHSIAGLVPPGRGLIEARPFRAPHHSVS
                     TAGLLGGGSHVIRPGELSLAHHGVLFLDEVTEFRRDLLEGLRQPLEDGRVVVVRASGA
                     VAFPARCTLVAAANPCPCGYQGDPRRQCTCSEARAAQYRHRLSGPLLDRLDIRLTVPR
                     LSKAELIGASAGEPSAAIRDRVEAARERQRARLRGTPFGANAAMPGSVARRHARLTSS
                     AERVLGSAVDRMGLSGRGFDRAMKVARTIADLEGSDRVTDAHVYEALRFRGVDREGVG
                     AA"
     gene            5149..6330
                     /gene="dprA"
                     /locus_tag="VF097_12160"
     CDS             5149..6330
                     /gene="dprA"
                     /locus_tag="VF097_12160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX6263590.1"
                     /translation="MPPERASPAPDEEVEARPGRAGAGDARSLPRGWPEGFAASEGDR
                     EALLRLSTMNVVPAELYARTWKEGSAAACLAATREPRSRGPRPTPGEILARLEACGGR
                     FLGVGDPGFPERLLDLPDPPAWLFVRGSVPLDVLAVAIIGARRCSSYGRETAEAIGRG
                     LAALGVAVVSGAALGIDGAGHRGALEGEGHTIAVLGSGVDVPYPPSNRDLIERIAVEG
                     ALISEYPPGTPANGFRFPARNRLIAALARSVVVVEGAAGSGSMITAEIATDVGREVYA
                     VPGNVTSRLAEVPNALIRDGAPMVRHAQDVLDCLRGEASHAFRALRDGDGGPPPDEEG
                     LNAEERRVLGALAAPATAEGVAGDAGLPLHRVLPVLAALELRGLVRDVGGRFERRSGE
                     A"
     gene            6635..7531
                     /locus_tag="VF097_12165"
     CDS             6635..7531
                     /locus_tag="VF097_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013126304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEX6263591.1"
                     /translation="MSRADHLIEDFCRHLTLERRLSPHTVSAYRRDLEDLDRFLRRGD
                     SSLAEATHALLRRWLAQLRTLGLARTTIARKAAAVRTFYAWTARRGHVEANPALLLSS
                     PKLPGRLPSVLKAGEAADLVEAPAGDDAWSLRDRAILEILYGTGIRVAELCGLDVEHV
                     DLDRRRVRVMGKGRKEREVPLGEPAAEALGEYVRSARGETMPERGGGSALFFNRRGRR
                     LGSRDVRALVERYGRDVLVGRRVSPHTLRHSFATHLMEGGADIRAVQELLGHASLDVT
                     QRYTHVSRRRLFGAYRASHPRA"
BASE COUNT         1006 a         2628 c         2945 g         1074 t
ORIGIN      
        1 atcaagacgt tcctcgtcca ggccttctac atcccatctg agtcgatggt ccccacgctg
       61 cgcaacggcg accgagtgct cgtcaacaag ctgatcttca acatccgtga cccggatcga
      121 ggggagatcg tcgtcttcga gaaccccaac ctccaggagg aggaccggaa cccctttgcg
      181 gccgtgtggc attggatcac cgagggcctg gggttcacgg tggatcccaa caaggacttc
      241 atcaagcggg tgatcgcgat ccccggcgac accatagaag tgcgcggggg ccgagtcttc
      301 gtcaacggtc agcggatccg ggagccgtac ctggtgcgga accgcgaccg ctccgacttc
      361 ggcccatact ccgttccgcc tgacacgtac ttcatgatgg gcgacaatcg agccaactcg
      421 caggactcca ggtccccggc ggtggggccg gtcccgaggg agaagatcgt ggggaaggcg
      481 ttcgtgatcc tctggccccc ctcgaggttc gagtggctcg gcgagggaga ctgaagaacc
      541 ggtacgagca gcgtctgcgc gagcgggggt tccggctcct cgcgggcgtg gacgaggcgg
      601 ggcgcggggc gctcgcgggc ccgctcgtcg ccgccgcggt gatcctcccg gaggacttcg
      661 acctcgatgg ggttcacgac tccaagctcc tgacgccgct ccagcgcgtc accgtctacc
      721 cgaggatcct cgaggccgcg ctggccgtgg ccgtgtgcag ggcgatgccg gggcggatcg
      781 accgcgaggg cctccaccgg acgaacctgc ggctgctccg ccgggcgatc cgcaccctcg
      841 atctcgagcc ggagtacgtc ctgaccgacg ggttccccgt ggggaggatg gacgtcccgt
      901 gcctggcgat gaaggggggg gacgcgctga acgggaccgt cgccgccgcg tcgatcgtgg
      961 ccaaggtgac ccgggaccgg acgatggagc ggtaccatcg ccgcttcccg gcctacggct
     1021 tcgatacgaa tcgaggctac ggcacccgca ggcactggtg ggccctggag cggtacggcc
     1081 ccacgccgat ccaccggctg tcgttcaagg gtgtcgggca ggcctcgctc ttcccccggt
     1141 tcgaggcgca taatggcgac gagggggagg ttcgatgagc ggcgaagagc tcgagcgcta
     1201 cgagaccgag atggagctcc agctctaccg ggagtaccgg gacgtgctcc cgatgttccg
     1261 ctacgtcgtc gagacggacc gccgcttcta cctcgccaac gaggtcgagg tcacccgcca
     1321 caccgagtcg ggcgagttcc tcatcgagat caacctcacg gacgcctggg tgtgggacat
     1381 gcaccggccc gctcgcttcg tggagcgggt ccgggtgctg accacccagg acgtcaacat
     1441 cgaggagatc tcgaagccag agccccccac cggcgactga cgttcgtgtc ggaccctcgc
     1501 ggcatgcttc gcgcatgcgg agcagggacc gcgtgtggca cgagggcgag cgggcggcct
     1561 ggggctggta ctgggagcgc ggctaccggc tcgtggcccg caactggcga tcccgtgtgg
     1621 gtgagctcga tctcgtcgtg gagcggggcg ggacgctcgt cttctgcgag gtgaaggcgc
     1681 gggcctcggc gctggccggc cccttcgacg cggtgacccc ggccaagcag cggaaggtcc
     1741 gggcggtggc cgaggccttc ctcctggcga cggggaccgg gccgcaggcc tgccggttcg
     1801 acgtggccag cgtggtgctg gggcggcgcg gggcccgggt gcacgtcttc gaggacgcgt
     1861 tctagagggt cgggacctgg tcccagtcga cgaagaagag gttggggtcg tcccggcgga
     1921 tctgagcggg gagctccagc cccgggcgga cgcggtcggc gacggcagac gggaccgcgg
     1981 cgtgtcccga gtccacgtgc gggggccgac cgtcgtccac ctcgatctgg acctcgatct
     2041 ccatgatccg gtagtcgccg acccggacgc ccgtgtcctt ggccttgcgg aacctggccg
     2101 tggcggggag ggggttctcg cggaccttgg cccgccgctc gtcgatctgg cgctggagct
     2161 ccaccgggtc cgtgccctgg gcggccgagg cgtcctgggc tccccagagg accaccacct
     2221 gggagaggtc gtccggatcc accctcaccg gcagggcggc ccccgaggtg aggatcccca
     2281 ccatcgcccc ggggatgaac tccttcttct tcaccatgta cgggtcccgg ccctcgacgt
     2341 ggacctcgag gtccatctcc acctgggggt tgatctcgtt gagggcgacg cccgtctggc
     2401 ggaaggactc gatcgtggcc gtccccggaa gcccggtggc gacgatctgc tgggcgcgac
     2461 gctgggacgt gcccaccgag cgggcccaga ggaggagcag gaggccggtg gcggcgagga
     2521 tggcccccgt cacggtcagc ccggtggccc cggcgcccgc ggagcgtccg gcgatcagga
     2581 accccacgcc gagtacgagc tcgaggatcg ccgtgccggc gaggacgttc cgcatccggt
     2641 tgccgcccca gctcggtccc tgcgcgatgg acatccgatc tccctccctc tgctcgtcgg
     2701 gagtgtgggg gcggcccgct cccgggtcaa gccctatcat cgcggcgtga gctacggagc
     2761 gacctcggcc acggctccgc tgaagcgcgt gctggtccgc ccgccgatgg agacggcggg
     2821 gtgggaggag ctcggctggc ggagcgagcc ggacctcgac cggatggtcc gggagcacga
     2881 ggggtacgtg gccgcgctgg aggcggccgg ggtcgaggtg gtcctgggga cgacgcgcgt
     2941 gccgggcaac tgcgacgcga tctacgcgtg cgacgtggcg atcgtgaccg agcgcggggc
     3001 gatcgtcctg cgtccgggga aggcggggcg acgggacgag cccgaggcgg tcgccgcgga
     3061 cctcgaggcg gccggcgttc cgatcatcgg ccggctcgag gagccggcca ccgcggaagg
     3121 aggggacctc ctgtggctgg acgagcgcac gctggtcgtg gggcgctcct accggacgaa
     3181 cgacgagggc atcgccgccc tgcgcgagct gctgcccgag gtcgaggtgg tctcggtcga
     3241 cctgccccac caccatggcg cgggtgtgat cctgcacctc ctgtcggtga tcagcatgct
     3301 ggacgaggac ctggcagtgg cccacccgcc cctgctgccc gtgcggctga tggagatgct
     3361 gggggagcga ggggtggggc tgatcgaggt gcccgacgag gagctcgact cccagggccc
     3421 caacgtgctg gcgctggccc cccgggtggc gctggccctg gaagggagcc cggtgacacg
     3481 gcgacggatg gaggcggcgg gggtcgacgt tcgcgtctat gccggcgagg acctctgcag
     3541 gaagggggac ggcggcccca cttgcctggc ccagccgatc ctccggggct gacgttcgtg
     3601 tcggaccctc gcgctaggtt tcgcccatgt acgcccgcgc ggttggggtg gcggtggtcg
     3661 gcgtccacgg ccatccggtc tcggtcgagg cccacatcgg gcgagggctc cccgcactcc
     3721 agctgaccgg cctacccggg gccgcggtcc aggacgcccg ggagcgggtg cgcccggcgg
     3781 tggagaactc cggcctcgag tggccgctgc gccgagtgac cgtgaacctc agccccgccg
     3841 acctccggaa ggaggggccg gggttcgacc tccccatcgc cctggcggtc ctggccgcga
     3901 cggcccagct cgaccaggag cgcctgcccg ggtacgcgtc ggcgggggag ctctcgctga
     3961 agggctccct cgtcccgacg ccgggcatct tggcggtggc ctgggcggcc cgggagtcgg
     4021 ggctgcgggg cgtgatcgtc cccgaggcga acgccgcgga ggccgctctc gtcgagggga
     4081 tcgaggtcgt gggggcgccg tcgctggcgc gggccgtcga gttcctgcgg gggaggtggg
     4141 agccggggcc cccctccgaa ccccctcccg gctccgtgga cggggccgcg gtggacttct
     4201 cggaggtgcg aggccaggcg gaggcccggc gagccctgga gatcgccgcg gccggcgggc
     4261 acaacgtgct gctgttcggc ccgcccggtg gagggaagac gatgctggcc cggagactcc
     4321 ccacgatcct cccggcgatg agccgcgacg aggccctcga agtgacgcgc ctccattcga
     4381 tcgccggcct ggtcccgccg ggccgcgggc tgatcgaggc acgcccgttc cgggcgccgc
     4441 accattcggt ctcgaccgcg ggcctgctgg ggggtgggtc gcacgtgatc cgccccggag
     4501 agctcagcct cgcccaccac ggcgtgctgt tcctggacga ggtgaccgag ttccgacggg
     4561 acctgctgga gggcctcagg cagccccttg aggacgggcg ggtggtggtc gtcagggcgt
     4621 cgggcgctgt ggcgttcccc gcacgctgca cgctcgtcgc ggcggcgaac ccgtgcccct
     4681 gcggatacca gggcgatccc cggagacagt gcacgtgctc ggaggcgagg gcagcccagt
     4741 acaggcatcg cctgtccgga ccgctgctcg accgcctgga catccgcctc acggtgccgc
     4801 gcctctcgaa ggccgagctg ataggagcgt cggcgggaga gccctccgcg gcgatccggg
     4861 atcgggtcga ggcggcgcgt gagcggcagc gggcccggct gcgggggacg ccgttcgggg
     4921 cgaacgcggc gatgcccggc tcagtggccc ggcggcatgc ccgcttgacc tcctccgcgg
     4981 agcgggtgct ggggagcgcg gtggaccgga tggggctctc ggggcgaggc ttcgaccggg
     5041 cgatgaaggt ggcccggacg atcgccgacc tcgaggggtc cgaccgtgtg accgacgccc
     5101 acgtgtacga ggccctgcgg ttccgggggg tcgatcggga gggcgtcggt gccgcctgag
     5161 cgcgcgtcgc ccgcgcccga cgaggaggtc gaggcccgcc cggggcgggc gggcgcgggc
     5221 gacgcgcgct ccctccctcg ggggtggccg gagggcttcg ccgcgtcgga gggagaccgg
     5281 gaggcgctcc ttcgactctc gaccatgaac gtcgtccccg ctgagctgta cgccaggacc
     5341 tggaaggagg ggagcgcggc cgcgtgtctc gccgcgaccc gggagccgcg gagccggggg
     5401 ccacgtccga ccccggggga gatcctggcc cggctcgagg cctgcggcgg gcgattcctc
     5461 ggcgtcggcg accccgggtt ccccgagcgg ctcctcgatc tgcccgaccc gccggcgtgg
     5521 ctgttcgtcc ggggctccgt cccgctggac gtcctcgcgg tggcgatcat cggggctcgc
     5581 cgctgctcct cctacgggag ggagaccgcc gaggcgatcg ggcgggggct cgccgcgctc
     5641 ggcgtggcgg tggtgagcgg cgccgccctg gggatcgacg gcgccgggca ccgaggagcc
     5701 ctcgagggcg agggccacac gatcgccgtg ctgggctcgg gggtggacgt cccctacccg
     5761 ccctcgaacc gggacctgat cgagcgcatc gccgtcgagg gcgcgctgat cagcgagtac
     5821 cctcccggga ccccggcgaa cgggttccgc ttcccggcgc ggaaccgcct gatcgccgcc
     5881 ctggccaggt ctgtcgtcgt ggtggaggga gcggccggca gcggctcgat gatcaccgca
     5941 gagatcgcga ccgacgtggg gagggaggtc tacgccgtgc ccgggaacgt cacctcgcgg
     6001 ctggcggagg tccccaacgc cctgatccgg gacggcgcgc ccatggtgcg gcacgctcag
     6061 gacgtcttgg actgccttcg gggcgaggcc tcgcacgcct tccgagcgct ccgggacggc
     6121 gacggggggc ccccgccgga cgaggagggg ctgaacgccg aggagcggcg cgtcctgggg
     6181 gcgctggctg ccccggccac ggcggagggc gtcgctggcg acgccgggct ccccctgcat
     6241 cgcgtgcttc ccgtgctggc cgccctggaa ctccgggggc tcgtgcgcga cgtggggggc
     6301 cggttcgagc ggcggtcggg agaggcctag aagcggagcg ccctccagac gtcgtccccg
     6361 cggtctcgga ggtcgccgag gtgaggcgcg ggtggacgcg gccggccgag caggcgctcg
     6421 agcaggcccc tgcgtctccg tgcccggccg accgaccggg cggccgcgtc cacccgcgcc
     6481 tcgcggatgc ggcgctgcag gtcgagctct cagacggggt ggccgaccac cggcgtgatc
     6541 atggccccat gctcccacat gcgctcgctc cggatgcttt gtctgcggtg cggcctgtga
     6601 tacacaggtg gcctcgcgga ccggaggctt ccccatgtcg cgcgctgacc acctcatcga
     6661 ggacttctgt cgccacctca cgctcgagcg gcggctgtcg cctcacaccg tgtcggcgta
     6721 ccgccgcgac ctcgaggacc tcgatcggtt cctgcggagg ggggactcgt cgctcgccga
     6781 ggcgacccac gcgctcctcc gtcggtggct cgcccagctg cggacgctcg gcctggctcg
     6841 caccacgatc gcccggaagg cggcggccgt caggaccttc tacgcctgga cggcccggag
     6901 gggacacgtc gaggcgaacc cggccctcct gctctcttcc ccgaagcttc cggggcggct
     6961 tccctcggtc ctgaaggcgg gcgaggccgc ggatctggtg gaggcccccg cgggcgacga
     7021 cgcatggagc ctccgggacc gggccatcct ggagatcctc tacgggaccg gtatccgcgt
     7081 ggccgagctg tgcgggctcg acgtggaaca cgtcgacctg gaccggcggc gggtccgggt
     7141 tatgggaaag gggcgcaagg agcgggaggt ccccctgggg gagcccgccg ccgaggctct
     7201 gggcgagtac gtccgctccg cgcgcggcga gaccatgccg gaacgcggag gggggtccgc
     7261 gctgttcttc aatcggaggg ggcgtcgcct cggatcccgc gacgtccgcg cattggtgga
     7321 aagatacggc agggacgtgc tggtggggcg ccgggtgagc ccgcacacac tccggcattc
     7381 gttcgccacg cacctgatgg agggaggggc cgacatccga gccgtgcagg agctcctggg
     7441 tcatgcgagc cttgacgtga cgcagcggta cacgcacgtc tcgcgacgcc ggctgttcgg
     7501 ggcctaccgg gccagccacc cgcgagcctg acgaggaggt cgaccgatcg ccagcgacca
     7561 gcaggccgtc gagcaggacg agctgaccgc gctctggaag gagtacaagg cgaacggatc
     7621 ccaggagatc cgggagcgcc tcatcctcca cta
//
LOCUS       DASUTZ010000282         5065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1802482, whole genome shotgun sequence.
ACCESSION   DASUTZ010000282 DASUTZ010000000
VERSION     DASUTZ010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1802482"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2383
                     /locus_tag="VF097_12170"
     CDS             <1..2383
                     /locus_tag="VF097_12170"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX6263592.1"
                     /translation="EYGALGLDAFEPWTPLDSVTIGKAIAFSLSFDVDIELTLDYLQY
                     VGTGQALGFDGHALFTQDAFRSQPFSDASTVPDATGASLATADRRTARAASKAAASLD
                     PDTVRLARGWLRRIERVPFLDPVLDEDLGAGSNEWGVAGSLTDSGFPMIANDPHLALD
                     MPSTFYPIRLRAGSMDVYGEGFAGAPGVILGRNRFISWGATTNPMDVTDTFQEQIVPD
                     PSSPSGLSTVFQGRLEPVIPIPEQYRANVGGNVAPVPPSPSIPPATLIVPRRNQGPIV
                     ALDAAAGTALSVQYTGFSGTRELETFLIWDRARNLEDFRRGLEAFDFGSQNWAYADVR
                     GNLAYFTSAEMPLREDLEAGTVNGLPPWFIRNGTGGNEWLPRTTTHPGQAIPYEILPP
                     EEMPQVVNPSAGFFVNANNDPAGTTLDNDPLNQLRPGGGIFYLNPGYDGFRAGRITEM
                     LRERIDGGERISFGDMQDMQSDVALIDAEVLVPHVLGAWERAQGSAIPELADLAADAG
                     VSEAVARLEAWDFMTPTGIPEGYDAADEGGQLSPPSQEEIADSVAATIYALWRSHVLG
                     NVIDGSLQGLGLDPPGSGQAMASLRHHLEAFDSAQGVGASGIDFFRVPGVADPEDERD
                     VLLLRSVREALDTLASPGMAAAFGGSSDQNDYRWGRLHRVVFDHPLGGPFNLPPAGGA
                     FPQPLPGLPGIPTDGGFGVVDASSHNPRARTVNGFMFGGGPVRRFVGEMAPGLARTRS
                     ESSLPGGTSGLLGDPRHFNLLPDWLTNEAYPQLLRTSDLRATLVSVQRLVPAS"
     gene            2517..3605
                     /locus_tag="VF097_12175"
     CDS             2517..3605
                     /locus_tag="VF097_12175"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX6263593.1"
                     /translation="MRPSSRSTIRVLAATVVAVFALASGPVAEADPPEQAQRRVDVMT
                     YNLYLGADLSPLFDPQVDTLPELIAAAAGVFAQTQQTDFPARAEVIAREIAEQSPSLI
                     GLQEVAIIQTAAITDPGTPLSTLDFLDILLTELEERGAHYESVATNVNFSGTLPISPT
                     TLATLTDRSVILARTDLPTSQLALSNPTSNTFEATLTLSAGGVPLEIIRGWSSIDVKL
                     RGKSFRFVNTHLEAFHPVIRQLQAAELIEWMGDSPLPILLAGDLNTLRGDPADAYGLF
                     VDAGFVDAWVDAMPGDPGFTAGQAADLMGPSLLDHTVDYVMYEGFLDAVTGSGEIIGE
                     EDPADRTPSGLWPSDHAGVNVAIHVASP"
     gene            3683..4963
                     /locus_tag="VF097_12180"
     CDS             3683..4963
                     /locus_tag="VF097_12180"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263594.1"
                     /translation="MGVRRIAVSIMLTLALLMIGAPGGASVPPANDDFASAIDVAALP
                     FEDTVDTTEATLEQDEPQPPCTFFTGNTVWYEFTPGADTIVSLDTLGSDFDSVLAVWT
                     GAELSSLDLVACNDDALSLSAQVSFRARSATTYHIQVGGFDSAAGSLLFRMRTVEAGV
                     IEGTVTREGTGAPLPGICVEALDRDLFSAQTVVTGADGTFEVVVRGGSYLVAFIDFCD
                     PSDDHVPEWYDDVAFSEPQSATPIDLSAGDVVSGIDAELTGGCPGIASLAAVLGFNQI
                     VGTAGPDDLVGTEDEDVLCGLGGDDTLKGLAGLDVLFGGPGRDRLLGGRGHDELIGSL
                     GKDELVGGPGADLLLGGRGNDRLRGGTGRDLLSGGPGRDRCVGGPGADLARGCEVTRS
                     VIRSRSRVIVRGSAGAISAREPRSLDELVAILRR"
BASE COUNT          715 a         1787 c         1802 g          761 t
ORIGIN      
        1 cgagtacggc gccctcgggt tggacgcgtt cgagccgtgg acgccgctgg acagcgtgac
       61 gatcgggaag gcgatcgcgt tctcgctgtc gttcgacgtg gacatcgagc tcacgctgga
      121 ctacctgcag tacgtgggga cggggcaggc gctggggttc gacgggcacg cgctcttcac
      181 ccaggatgcg ttccgctcac agcctttctc cgacgcctcg acggtgccgg acgccaccgg
      241 tgcctcactc gcgacggccg accgccggac ggcccgcgcc gcgtcgaagg cggcggcgag
      301 cctcgacccg gacacggttc gtctggcgcg cggctggctg cgacggatcg agcgcgtccc
      361 cttcctcgat ccggtcctgg acgaggacct cggggcgggc tcgaacgagt ggggtgttgc
      421 ggggtcgctc accgactcgg gcttcccgat gatcgccaac gacccacacc tcgccctcga
      481 catgccctcg acgttctatc cgatccgcct ccgggccggc tcgatggacg tctacggcga
      541 gggcttcgcg ggcgcgcccg gggtcatctt ggggcgcaac cgcttcatct cgtggggcgc
      601 caccacgaac ccgatggacg tgaccgacac gttccaggag cagatcgtcc ccgacccgag
      661 ctccccgagc gggctgagca ccgtgttcca gggccggctg gagccggtca tcccgatccc
      721 cgagcagtac cgcgcgaacg tgggcgggaa cgtggctccc gtgccgccga gcccgagcat
      781 cccgccggcc accctgatcg tcccccgccg gaaccagggg ccgatcgtcg ccctcgacgc
      841 cgcggccggg acggcgctct cggtgcagta cacggggttc agcgggacgc gggagctgga
      901 gacgttcctg atctgggacc gcgctcggaa cctggaggac ttccgccggg ggctcgaggc
      961 cttcgacttc gggtcgcaga actgggccta cgccgacgtc cgcgggaacc tggcttactt
     1021 cacaagcgcc gagatgccgc tccgcgagga cctcgaggcg ggcactgtga acgggctgcc
     1081 gccgtggttc atccggaacg gcaccggggg caacgagtgg ctgccgcgga ccacgacgca
     1141 tccaggccag gcgatcccgt acgagatcct cccgcccgag gagatgccgc aggtggtgaa
     1201 cccctcggcg gggttcttcg tcaacgccaa caacgacccg gccggcacca cgctcgacaa
     1261 cgaccccctg aaccagctcc ggccgggcgg ggggatcttc tacctgaacc cgggctacga
     1321 cgggttccgc gcgggccgca tcaccgagat gctccgcgag cggatcgacg ggggcgagcg
     1381 gatctcgttc ggggacatgc aggacatgca gtccgatgtg gcgctgatcg acgccgaggt
     1441 gctggtgccc cacgtgctcg gggcgtggga gcgagcgcag gggagcgcca tccccgagct
     1501 cgccgatctg gccgcggatg cgggcgtctc agaggcggtg gcgcgtctgg aggcctggga
     1561 cttcatgacc ccgacgggga tccccgaggg atacgacgcc gctgacgagg gcgggcagct
     1621 ctcgccgccg tcacaagagg agatcgcgga cagcgtggcg gccacgatct acgcgctctg
     1681 gcggagccac gtgctgggca acgtgatcga cgggagcctg cagggcctgg ggctcgaccc
     1741 gcccgggagc ggccaggcga tggcgtcgct ccgccatcac ctcgaggcct tcgactcggc
     1801 gcagggcgtc ggggcctccg gcatcgactt cttccgggtg cccggggtgg ccgaccccga
     1861 ggacgaacgg gacgtgctcc tccttcgcag cgtccgcgag gcgctcgaca ccttggcctc
     1921 cccgggcatg gctgcggcgt tcgggggctc gagcgaccag aatgactacc ggtggggaag
     1981 gctgcaccgg gtcgtcttcg atcatccgct gggcgggccc ttcaacctgc cgcccgccgg
     2041 cggggccttc ccccagccgc tgcccggcct ccccgggatc cccacagacg ggggcttcgg
     2101 cgtggtggac gcgtccagcc acaacccgag ggcgaggacg gtgaacggct tcatgttcgg
     2161 gggagggccg gtccggcgct tcgtcggcga gatggccccc gggctggcga ggacccgatc
     2221 cgagtcgagc ctgcctggag ggacgagcgg gctgctcggc gatcctcgcc acttcaacct
     2281 gctgccggac tggctgacga acgaggccta cccacagctc ctccgcacga gcgacctccg
     2341 ggccacgctc gtctcggtgc agcggctcgt tccggcctcc tgagggtgag cggacccaac
     2401 tcccggccga ggaggcgccc cgatgcttcc gggggcgtcg tctgacgccc ggcgtgttgt
     2461 cagggtaggc cctcggcgtt agagtcgcgg gcacgcttcc gggcagggag gttcacatgc
     2521 gaccgtcttc acgctcaacg atccgcgttc tcgccgccac cgtcgtcgcg gtcttcgcgc
     2581 tcgcgtcggg cccggtggcc gaggccgacc caccggaaca ggctcagcga cgcgtcgacg
     2641 tcatgaccta caacctctac ctcggggccg atctctctcc gctcttcgat ccccaagtgg
     2701 acacgctgcc tgagctgatc gcggcggccg ccggcgtctt cgcccagacc cagcagaccg
     2761 acttccctgc ccgggccgag gtcatcgcgc gggagatcgc cgagcagtcg ccgtcgttga
     2821 tcgggctcca agaggtggcc atcatccaga cggcggccat caccgacccc ggcaccccgc
     2881 tcagcacgtt ggacttcctc gacatcctgc tcacggagct cgaggagcgc ggggcccact
     2941 acgaatcggt cgccaccaac gtgaacttca gcgggaccct gccgatcagc cccaccacgc
     3001 tggccaccct gaccgatcgc agcgtcatcc tggccaggac cgatctcccc acctcgcagc
     3061 tcgcgctgtc caacccgacg tcgaacacct tcgaggccac gctgacgctc tcggcggggg
     3121 gcgtgccgct cgagatcatc cgcggctggt ccagcatcga cgtgaagctc cgcgggaagt
     3181 ccttccgctt cgtgaacacg cacctggagg cgttccatcc ggtgatccgg cagctccagg
     3241 cggccgagct gatcgagtgg atgggcgact cgcccctgcc cattctcctc gccggagacc
     3301 tcaacacgct gcggggggac ccggcggatg cctacggact gttcgtcgac gcgggcttcg
     3361 tggacgcctg ggtggacgcg atgccgggcg atccggggtt caccgcgggg caggcggccg
     3421 acctgatggg gccctcgctc ctcgaccaca cggtggacta cgtcatgtac gagggcttcc
     3481 tcgacgcggt gaccgggagc ggggagatca tcggtgagga ggatcccgcc gaccgcaccc
     3541 cgtcggggct gtggccctcg gaccatgccg gcgtgaacgt cgcgatccac gtggcgagcc
     3601 cctgagacgg aacagacgga cactccttga cggtcccgcc gatagggggg atgctgtgcc
     3661 tccatctcga agggggccgc tcatgggggt tcgccggatc gccgtctcga tcatgctcac
     3721 gctcgccctg ctgatgatcg gagcgcccgg gggcgcgtcc gtgccgccgg ccaacgacga
     3781 cttcgcctcg gctatcgacg tcgccgccct gcccttcgag gacaccgtgg acacgaccga
     3841 ggccacgctc gagcaggacg agccccagcc tccgtgcacg ttcttcacgg ggaacacggt
     3901 ctggtacgag ttcacgcccg gggccgacac catcgtttcc ctcgacacgc tggggagcga
     3961 cttcgactcg gttttggccg tctggacggg agccgagctg agctccctgg acctggtggc
     4021 ctgcaacgac gacgccctct ccctcagcgc gcaggtctcg ttccgcgccc gctccgcaac
     4081 gacctaccac atccaggtgg gcgggttcga cagcgcggcg ggttccctcc tcttccggat
     4141 gcgaacggtt gaggcggggg tgatcgaggg gaccgtcacg cgggagggga cgggggcccc
     4201 gctccctggg atctgcgtgg aggcgctcga cagagacctg ttctcggcgc agaccgtggt
     4261 caccggggcc gacggcacct tcgaggtcgt cgtccgcggg ggctcgtacc tggttgcctt
     4321 catcgacttc tgcgatccct cggacgatca cgtccccgag tggtacgacg acgtcgcgtt
     4381 ctccgagccc cagagcgcca cgcccatcga cctgtccgcg ggggacgtgg tcagtgggat
     4441 cgacgccgag ctcaccggcg gttgccctgg gatcgcctcc ctggcagcgg tgctgggctt
     4501 caaccagatc gtggggaccg ccggtccgga cgacctggtg gggaccgagg atgaggacgt
     4561 gctgtgcggg ctcggggggg acgacacgtt gaaggggctg gccgggttgg acgtgctgtt
     4621 cggcggcccg ggcagggacc gcctcctcgg tggccggggg cacgacgagc tgatcggcag
     4681 cctgggcaag gacgagctcg tcggaggtcc cggagcggat ctcctcttgg gagggcgggg
     4741 caacgaccgg ctccgcgggg ggaccggcag agatctcctc tccggcgggc ccgggcgcga
     4801 caggtgcgtc ggaggaccgg gagcggacct cgctcgggga tgcgaggtta cccggagcgt
     4861 catccgctcg aggtctcgtg tcatcgtccg cgggtcggcc ggggccatca gcgcgaggga
     4921 accccgctcg ctcgacgagc tcgtcgcgat cctgcgtcgg tgaccgccgg aggggccgcg
     4981 ctccggcgcg gcccctccgg cggtcacccg gtgttggggg cgccgggcgt caccgtcgcg
     5041 gtgaaggccc agtcggtggc cgcat
//
LOCUS       DASUTZ010000283         7209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_899743, whole genome shotgun sequence.
ACCESSION   DASUTZ010000283 DASUTZ010000000
VERSION     DASUTZ010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7209
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899743"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VF097_12185"
     CDS             <1..414
                     /locus_tag="VF097_12185"
                     /inference="COORDINATES: protein motif:HMM:NF025109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4162 domain-containing protein"
                     /protein_id="HEX6263595.1"
                     /translation="LADDIVVIDRGQKIAHGTADQLKLQVGGERLELVLGDMSDLESA
                     RSILETVCTDEVQVTEHTRSLTTAVRGGSEDLRRVLDLLQQRSVQIDDIGLRRPTLDD
                     VFLTLTGHSAGVANGGDGDGQVPDAERIEEREVAR"
     gene            411..1214
                     /locus_tag="VF097_12190"
     CDS             411..1214
                     /locus_tag="VF097_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6263596.1"
                     /translation="MTTLTMALGDGLVVAKRNLIKIKRVPEILIWTTMSPIMFVLLFA
                     YVFGGSIDTGNVSYREYLIAGVFVQTVIFGSTFTGAGLADDITKGIIDRFRSLPMSRS
                     AVLVGRTTSDILYNSISIAVMSIAGLIVGWGIRTSLLPAIGGFLLLLLFAYAASWIMA
                     FVGLKVSSLEVFQNASFMFIMPVTFVANTFVLSQSLPGPLRVFAEWNPVSAVAQAVRE
                     AFGNTATDVPLPDAWSLQNPVLYSLIWVGIILLIFVPLSIRQFRKAATR"
     gene            1345..2253
                     /locus_tag="VF097_12195"
     CDS             1345..2253
                     /locus_tag="VF097_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263597.1"
                     /translation="MELRREPISLGTVIEFVGYAGAAVALSAASTALGQEAGEGVRIA
                     FDLITAVVLLAAGWAIAGRPEPYPRMQSVFWFVAVFSVVDLAGVLFGEVIEGLDERMV
                     VFLTALVAALSSLVLWWVLRRSLQVLALILTGYIAIISLVFPDPGDFILGPPDLSAVG
                     FVTWLLGGGVIFLGWLGWLGPRRTALAVGSILALIGPFFFLIGGEEIVGEILALATAF
                     ALMVLGSWVEDRGVAGLGIAGTLIVASTIVANHVEDQGPAIAVLVVGLLLLGAAILVA
                     RGSIGGPSALEEGEAPPPPPPAPPPA"
     gene            2309..3208
                     /locus_tag="VF097_12200"
     CDS             2309..3208
                     /locus_tag="VF097_12200"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020667539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX6263598.1"
                     /translation="MPDAVRELVRDGDTVAIEGFTHLISFAAGHEIIRQGRRDLTLCR
                     LTPDLIYDQMVAAGCARRLVFSWLGNPGVGSLHAVRRRVEGREGDPLELEEYSHFGMV
                     CRYVAGASRLPFFPLRSYEGSDLPKVNPRIVPIESPYDPSERLYAVPPLNPDVVVVHA
                     QRADAEGNAQIWGLLGVQREAAFAGERVLVVCEELVEPKVIRADPNRTVIPGLIVDAV
                     VAAPRGCHPSFAQGYYDRDNRFYREWDAISRDPERLEVWLDEWVRGVSSHEEYAEKVG
                     EERWAELTPGEAWSGRVNYGSYE"
     gene            3205..3957
                     /locus_tag="VF097_12205"
     CDS             3205..3957
                     /locus_tag="VF097_12205"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004595063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX6263599.1"
                     /translation="MTDWTPTEMMVVASARQLAGERVCFVGVGPPNIACNLARRTVSP
                     GLQLVYEAGVFGARPARLPLSIGDPSLATGSDAIHGMFELFAFYLQGGLIDVGFLGAS
                     QIDRHGNINTTVIGDYGAPKVRLPGSGGACEIAISAGKVFVIMPQSRRSFVERIDFTT
                     SPGHLAGRRPHGWIGAGPTVVVTDLGVYGFDASGEMELRSLHPNVPLERVREETGWEL
                     RVSESLSETPSPTEQELRLIREELDPEGVATR"
     gene            3968..5017
                     /locus_tag="VF097_12210"
     CDS             3968..5017
                     /locus_tag="VF097_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEX6263600.1"
                     /translation="MSERVLSTRALNRALLARQRLLERARAPLPRALEQVGGIQAQYA
                     PSMYVGLWSRLEGFSRDALTRALERRTVVQATVMRATIHLVSARDYPVLTEGVRRQRR
                     ASWLRAQPGRLDGVDMERAAATVRCALREGPRRQADLTALLEDEGFPRPAWSAVGLWV
                     DMVRVPPSGTWERRRADLYGLAEDWLDLGEPSEEQGIELLLRRYLRGFGPATANDAAS
                     WTGIPVSTLAPFLERMRLVRFRDEEGRELLDLPRAPRPDPDTSAPVRFLPTWDATLLA
                     HARRTQILPEGYRPRIFHTRNPQSVPTFLVDGSVAGSWRYEDGRVRLDPFERLSRARM
                     AEIEEEAERLAAFHA"
     gene            5068..5952
                     /locus_tag="VF097_12215"
     CDS             5068..5952
                     /locus_tag="VF097_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX6263601.1"
                     /translation="MRRAATIAAVLPVALLLLAAPTSAQEEHGDDQVVLTGEITIEEG
                     RTVDGVVILNGPATIDGTVRGSVVAFNGEVTISGLVEDDVIVFNGPTTVRSGAEIGGD
                     LITREAAVVEEGATVRGAVRGRPFDLTRGPFPFFARVAVWAALTVSLLVLGLLLVLLF
                     PRAPDAVDLAWRTAMGGAIGLGLLLLIGLPIVGVLIAITIVGVPLGVALLLALALIYA
                     IGYVSAGWVLGRMLIKPPTTALLAALVGILVLRLVALVPALGGIVGTLASAFGLGAIA
                     VAAWRAGRDRPTTEGGAA"
     gene            5949..6746
                     /locus_tag="VF097_12220"
     CDS             5949..6746
                     /locus_tag="VF097_12220"
                     /inference="COORDINATES: protein motif:HMM:NF021615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HEX6263602.1"
                     /translation="MSERGLELRARTVGEILTDAFEVYRRNFFVLVSIAAVVVVPLTL
                     FQYLLFVEVADVWGATPGEIRSLDAAEAWRVTLGGFALGLISFVVSQLATGAGTHAVV
                     SAVAGGEPGVGVSYRAALGRLGSLLLATLLMGLAVGVGFVLFIIPGFIVLVRLIATIP
                     AVVVERATGGRALSRSWELVRGRSWPVFGAMLVVLLVTGILTGIVTRPVGDHWFAQGL
                     AASIASVLTAPYVVTVVVLLYLDLRVRKEGLDPTTLRHELVEGRGPA"
     gene            6793..7161
                     /locus_tag="VF097_12225"
     CDS             6793..7161
                     /locus_tag="VF097_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017791817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6263603.1"
                     /translation="MATGMDIMQLASENEDFRREVITGEHGQVVLMTIAANGEIGEEV
                     HDDVDQILLFVAGRGEAVLDGQTGPVGPNDMVFVRAGTRHNFRNVGDEPLRVVTVYAP
                     PEHPAGTVHRTKEEADAAEH"
BASE COUNT          932 a         2444 c         2645 g         1188 t
ORIGIN      
        1 ctggccgacg acatcgtcgt gatcgaccgc ggccagaaga tcgcccacgg cacggccgac
       61 cagctcaagc tgcaggtcgg cggggagcgg ctcgagctcg tgctgggcga catgtccgac
      121 ctggagagcg cccgctcgat cctggagacc gtctgcaccg acgaggtgca ggtcacggag
      181 cacacgagga gcctgacgac cgcggtgcga ggcgggtctg aggaccttcg acgggtgctg
      241 gacctcctcc agcagcggtc ggtgcagatc gacgacatcg ggcttcgtcg tcccaccctc
      301 gacgacgtgt tcctcacgct gaccgggcac tcggcgggcg tggccaacgg cggggacggc
      361 gacggccagg tgccggacgc cgagcggatc gaggagaggg aggtggcacg atgaccacgc
      421 tgacgatggc cctcggcgac ggcctcgtcg tcgccaagcg caacctcatc aagatcaagc
      481 gggtccccga gatcctgatc tggaccacga tgtccccgat catgttcgtg ttgctgttcg
      541 cgtacgtgtt cggagggtcg atcgacacgg gcaacgtgag ctaccgcgag tacctgatcg
      601 cgggtgtgtt cgtccagacg gtgatcttcg ggtcgacctt cacaggtgcg ggcctcgccg
      661 acgacatcac gaaggggatc atcgaccggt tccggtcgct cccgatgtcg cggtcggcgg
      721 tgctcgtcgg ccgcacgacc agcgacatcc tctacaactc gatctcgatc gcggtgatgt
      781 cgatcgccgg cctgatcgtg gggtggggga tcaggacgag cctcctgccg gcgatcggtg
      841 gcttcctcct gctcctgctg ttcgcctacg cggcttcgtg gatcatggcg ttcgtcggcc
      901 tcaaggtgtc gagcctggag gtcttccaga acgcctcgtt catgttcatc atgccggtga
      961 cgttcgtggc caacacgttc gtcctgagcc agagcctgcc cgggcccctg cgcgtgttcg
     1021 cggagtggaa cccggtctcg gccgtggctc aggcggtgcg ggaggcgttc gggaacactg
     1081 ccaccgacgt cccgctgccg gacgcctggt ccctccagaa cccggtgctc tacagcctga
     1141 tctgggtcgg gatcatcctg ctgatcttcg tcccgctctc gatccgccag ttccggaagg
     1201 ctgccacgcg ctgagctagg gggtcgagga cttgcgctcg gaggcccggg tccgcgatgc
     1261 gggcccgggc ctccgagcgc tccggtcctt ggtgtgctcg cctcgccgtg tcatccttcg
     1321 gtcatccgcg ggagggggag ggacatggag ctgaggcgcg agccgatctc gctggggacg
     1381 gtcatcgagt tcgtcgggta tgcgggcgcc gcagtggcgc tgagcgcggc ctcgaccgcg
     1441 ctcggtcagg aagcaggaga gggcgtccgc atcgccttcg acctgatcac ggccgtggtg
     1501 ctgctggcgg cgggttgggc gatcgccggg cggcccgagc cgtacccgcg gatgcagagc
     1561 gtcttctggt tcgtggccgt gttctcggtg gtcgatctgg ccggggtgct gttcggcgag
     1621 gtgatcgagg ggctcgacga gcggatggtg gtgttcctta ccgccctcgt ggcggctctc
     1681 tcctccctcg tgctgtggtg ggtactgcgt cggagcctgc aggtcctggc gctcatcctc
     1741 accggctaca tcgcgatcat ctcgctcgtg ttcccggatc ccggggactt catcctcgga
     1801 ccgcccgacc tcagcgcggt cggtttcgtg acgtggcttc tcggaggggg cgtgatcttc
     1861 cttggctggc tcggctggct gggtccgcgc aggaccgcgc tcgcggtcgg ctcgatcctg
     1921 gcgctgatcg gccccttctt cttcctgatc gggggcgagg agatcgtggg cgagatcctg
     1981 gcgctggcca ccgccttcgc gctcatggtc ctcggctcct gggtcgagga tcgcggcgtc
     2041 gccggcctgg ggatcgcggg gaccctgatc gtggcgtcca cgatcgtcgc caaccacgtc
     2101 gaggaccagg gcccggccat cgccgtgctc gtcgtggggc tgctcctcct cggcgcggcg
     2161 atcctggtgg cgcgcgggag catcgggggt ccctccgccc tcgaggaggg cgaggcgccc
     2221 ccgcccccgc ctcccgctcc gccccccgca tagcgcgtcc ggtcggacgc cgatggtgta
     2281 gcgtccgctc gtgtcgaagg tgacctcgat gcccgacgcc gtgcgggagc tcgtccgcga
     2341 cggcgacacc gtggcgatcg aggggttcac gcacctgatc tcgttcgccg ccgggcacga
     2401 gatcatccgc caggggcgcc gcgacctcac gctgtgtcgc ctcacgcccg acctgatcta
     2461 cgaccagatg gtggcggccg ggtgcgctcg gcggctcgtc ttctcctggc tggggaaccc
     2521 gggcgtgggg tccctccacg cggtgaggcg ccgggtggag ggccgggagg gggatccgct
     2581 cgagctcgag gagtactcgc atttcggtat ggtgtgccgc tacgtggctg gggcgtcgcg
     2641 gctccccttc ttccccctca ggagctacga ggggagcgac ctcccgaagg tcaacccccg
     2701 catcgtcccg atcgagagcc cctacgaccc gagcgagcgt ctctacgccg tgcccccgct
     2761 caaccccgac gtggtcgtgg tgcacgccca gcgcgccgac gcggagggca acgcgcagat
     2821 ctggggtctc ctcggggtcc agcgggaggc ggccttcgcc ggggagcggg tcctcgtggt
     2881 ctgcgaggag ctcgtcgagc ccaaggtgat ccgggccgat ccgaaccgca cggtgatccc
     2941 cggcctgatc gtcgacgcgg tggtggcggc gccccgagga tgccacccgt ccttcgccca
     3001 gggctactac gaccgcgaca accgcttcta ccgggagtgg gacgcgatct cgcgtgaccc
     3061 ggaacgcctc gaggtctggc tcgacgagtg ggtgcggggc gtctcgtcgc acgaggagta
     3121 cgcggagaag gtgggggagg agcgctgggc cgagctgacg cccggggagg cctggtcggg
     3181 ccgcgtgaac tacgggagct acgagtgacc gactggacgc ccaccgagat gatggtcgtg
     3241 gccagcgccc ggcagctcgc cggtgagcgg gtgtgcttcg tgggcgtcgg gccgcccaac
     3301 atcgcctgca acctggcccg gcgaacggtc agcccgggtc tccagctcgt ctacgaggcc
     3361 ggtgtgttcg gagcccgccc ggccaggctc ccactgtcga tcggcgatcc gtcgctggcc
     3421 acgggctccg acgcgatcca cgggatgttt gagctgttcg ccttctacct ccaggggggg
     3481 ctgatcgacg tcggtttcct gggcgcctcc cagatcgatc gccacggcaa catcaacacg
     3541 accgtgatcg gcgactacgg agctccgaag gtgcgtctgc ccgggtccgg cggcgcctgc
     3601 gagatcgcga tcagcgcggg gaaggtcttc gtgatcatgc cgcagtcgcg gcgctcgttc
     3661 gtcgagcgga tcgacttcac gaccagcccc ggacacctcg ccggaaggag gccgcatggt
     3721 tggatcgggg cagggcccac cgtcgtcgtc accgacctgg gcgtgtacgg gttcgacgcc
     3781 tcgggcgaga tggagctccg gagcctccac ccgaacgtcc ccctcgagcg ggtccgagag
     3841 gagaccgggt gggagctgag ggtctcggag tctctctccg agaccccgtc ccccaccgag
     3901 caggagctcc ggctgatccg cgaggagctc gaccccgagg gggttgccac ccgttaggct
     3961 cgccgcgatg agcgagcgcg tgctctcgac ccgggcgctg aaccgggccc tcctggcgcg
     4021 gcagcgcctg ctcgagcgcg cgcgggctcc gctcccgcgc gcgctcgagc aggtcggcgg
     4081 gatccaggcc cagtacgccc cctcgatgta cgtcggcctc tggtcgcgcc tcgaggggtt
     4141 cagcagggac gcgctcaccc gagcgctgga gcgtcgcacg gtggtccagg ccacggtgat
     4201 gcgggcgacg atccacctgg tctcggcccg cgactacccg gtgctcaccg agggcgtccg
     4261 gaggcagcgc cgggcgtcgt ggctcagggc tcagccgggc cggctggacg gggtcgacat
     4321 ggagcgggcc gctgcgaccg tgcgctgcgc cctgagggag ggccctcggc gtcaggccga
     4381 cctcacggcg ctcctggagg acgagggctt ccctcgcccg gcctggtccg ccgtcgggct
     4441 ctgggtcgac atggtccgcg tgcccccgtc ggggacgtgg gagcgacgcc gcgccgacct
     4501 gtacggcctg gcggaggact ggctcgatct gggggagccc agcgaggagc agggcatcga
     4561 gctcctcctc cgcaggtacc tccgcggctt cggcccggcc acggcgaacg acgccgcgag
     4621 ctggacgggc atccccgtct ccacgctcgc tcccttcctg gagcgcatgc gcttggtgcg
     4681 gttccgggac gaggagggac gagagctcct cgacctcccc agggcgccgc ggccggaccc
     4741 cgacacctcc gcgccggtcc gcttcctccc gacctgggac gccacgctcc tggcccacgc
     4801 ccgccgcacg cagatcctcc ccgaggggta ccgccctcgg atcttccata cgaggaaccc
     4861 ccaatcggtc ccaaccttcc tcgtggacgg ctcggtggcc ggctcctggc ggtacgagga
     4921 cggccgggtg cggctcgatc cgttcgagcg cctctcgcga gcgaggatgg ccgagatcga
     4981 ggaggaggcg gagcggctcg ccgccttcca cgcgtgaggt tgcctcgccc cgagaagtgt
     5041 gggatgaccc caagagcagc cgatgccatg agacgcgccg ccacgatcgc cgccgtgctc
     5101 cccgtcgcgc tcctgctgct cgccgcgccg acatccgcgc aggaggagca cggcgacgat
     5161 caggtcgtcc tgacggggga gatcacgatc gaggagggca ggaccgtcga cggcgtcgtg
     5221 atcctcaacg gcccggccac gatcgacggc acggtccggg ggtcagtggt cgccttcaac
     5281 ggcgaggtga ccatctcggg tctcgtcgag gacgacgtca tcgtgttcaa tggcccgacc
     5341 acggtacggt cgggggcgga gatcgggggc gacctgatca cgagggaggc ggccgtcgtg
     5401 gaggagggag cgaccgtccg tggagccgtc cgcgggcgtc ccttcgacct gacgaggggc
     5461 cccttcccct tcttcgcgcg cgtcgcggtg tgggcggccc tgacggtctc gctgctcgtc
     5521 ctcgggctcc tgctcgtgct gctgttcccg agggcgccgg acgccgtgga cctcgcgtgg
     5581 cggacggcca tgggcggggc gatcggtctg ggactcctgc tcctgatcgg gctcccgatc
     5641 gtgggggtcc tcatcgcgat cacgatcgtg ggcgtgccgc tgggggtggc cctcctgctc
     5701 gccctggcgc tgatctacgc gatcgggtac gtgagcgccg ggtgggtgct ggggcggatg
     5761 ctgatcaagc ccccgacgac ggcgctgctc gccgctctgg tcgggatcct ggtcctgcgg
     5821 ctggtcgcac tcgtcccggc gttgggcggc atcgtgggca ccctggcctc ggcgttcggt
     5881 ctcggagcga tcgcggtcgc ggcctggagg gcgggacgag accgccccac gacggaggga
     5941 ggtgcggcat gagcgagcga ggcctggagc tccgggcccg gacggtgggg gagatcctga
     6001 cggacgcgtt cgaggtctac aggaggaact ttttcgtgct ggtgtcgatc gcggcggtgg
     6061 tcgtcgtccc cctcacgctg ttccagtacc tcctgttcgt cgaggtcgcg gacgtatggg
     6121 gggcaacccc gggcgagatc cggtccctcg acgccgccga ggcctggagg gtcaccctgg
     6181 gagggttcgc gctgggactc atcagcttcg tggtctccca gctcgccacc ggggccggga
     6241 cgcacgccgt cgtctcggcg gttgccgggg gcgagcccgg cgtcggagtg agctaccgcg
     6301 cggccctggg gaggctgggg tcgctcctct tggccacgct cctgatgggc ctcgcggtgg
     6361 gagtcgggtt cgtcctgttc atcatcccgg gcttcatcgt gctcgtgcgg ctgatcgcca
     6421 cgatccctgc cgtcgtggtc gagcgggcga ccggggggcg ggcgctctcg agatcgtggg
     6481 agctggtgag gggccgctca tggccggtgt tcggggcgat gctcgtcgtc ctcctcgtca
     6541 cgggcatcct gacgggcatc gtcacgcgtc ccgtcggcga tcactggttc gcccagggcc
     6601 tggccgcgtc gatcgcatcg gtcctgacgg cgccctacgt ggtcaccgtc gtcgtcctcc
     6661 tgtacctgga cctccgggtg aggaaggagg gcctcgaccc tacgaccctc cggcacgagc
     6721 tggtcgaggg gcgcgggccc gcatgacccc tccgcggcga gggtaaggaa gcgagcagtg
     6781 agaggaggcg ccatggcgac ggggatggac atcatgcagc tggcttccga gaacgaggac
     6841 ttccgtcgag aggtgatcac cggtgagcac ggccaggtcg tcctcatgac gatcgccgcg
     6901 aatggggaga tcggggagga ggtccacgac gacgtggacc agatcctgct cttcgtcgcc
     6961 gggcgaggag aggccgtgct cgacggccag accgggcccg tgggccccaa cgacatggtc
     7021 ttcgtccgcg ccgggacgcg gcacaacttc cgcaacgtgg gagacgaacc gctccgtgtg
     7081 gtgacggtct acgcgccccc ggagcacccg gcggggaccg tccaccggac caaggaagag
     7141 gccgacgcgg ccgagcactg agcgcacgca ttgctctagg cttgtcccgt ccttcgatct
     7201 gggggtgac
//
LOCUS       DASUTZ010000284         4700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1678819, whole genome shotgun sequence.
ACCESSION   DASUTZ010000284 DASUTZ010000000
VERSION     DASUTZ010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678819"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1341
                     /locus_tag="VF097_12230"
     CDS             127..1341
                     /locus_tag="VF097_12230"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX6263604.1"
                     /translation="MPRVPLLSYWRAEQRTIRQGLVAMLLASVAMLGAGVALGFITDT
                     LESLPGLMVMVPAAIAARGNVFGALASRLGTAIHAGLFDTTRRREGVLYQNVFAATAL
                     TLSISLLMAVLARTLSATFGVTSISIIDFVLISILGGVVASVIVLGLTIVLAVTAQRR
                     GWDLDSVAAPLITAAGDVLTVPAIFLATFAVGFRWVSPTLTAILTLVTLAVTIRAAIT
                     DLRATRRIVRESLPVLTLAATIDILAGLMIESQLEQFVTFPVLLILIPPILGDAGGLG
                     GILASRLASKLHLGVITPRGLPEAAALSDLSIVALFAVVVFPLVGVAAHVVAGLFGIA
                     SPGMGTVVGVSLLAGLLATAAASVVAYYAAIASFRMGMDPDSHGIPLITSSMDLAGVV
                     ALILAIFAFGIG"
     gene            1371..2567
                     /locus_tag="VF097_12235"
     CDS             1371..2567
                     /locus_tag="VF097_12235"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HEX6263605.1"
                     /translation="MDDSPRRNVKDLLAEAKDTSELMVDLAYAAVFFNDEDLADEVDA
                     LEELMQGYIHDLNWISMLAARSPEDAEAMAGVLAIARAIESIGDAAADIARVVTVRLG
                     IPTTLRADLRHADEIVGRVKVREESEVVGRSLRDLTLPTATGMWVIAIRRGMEWETDP
                     GPDSVVNAGDVLLYQGPEEGANLLRETAGAPPLPSPPDEEPLALSELDRAVDLLVEMK
                     NSAEVAVGLAYSSLLFREPALAAEVAALETRSDTLLDELESWVLRASPEARNPDELRG
                     LLRLGYASEMVFDAAREMTRAVESGDELHPVVQAALEATDEVAVETVVEAGSGAEGQT
                     LRELSLETEAGMLALAIQRGRRWIHRPRPRFQLEAGDRVIAIGPEDGIPGLEALTSAP
                     AETNAG"
     gene            complement(2568..3521)
                     /locus_tag="VF097_12240"
     CDS             complement(2568..3521)
                     /locus_tag="VF097_12240"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX6263606.1"
                     /translation="MLREVTLAAPRGYCAGVERAVDTVERALQLYGAPIYVRKQIVHN
                     LHVVRDLESKGAIFVEEETEVPEGATVVLSAHGVAPEVYRNAATRGLNVIDATCPLVT
                     KVHLEARRFAKEGYTILLIGHEGHEEVVGTSGEAPEHIRLVGSKDEARTVEVKDPSKV
                     AYLSQTTLSVDETNEIIEVLRERFPSIVGPPRDDICYATQNRQDAVKSLAREVDLVLV
                     IGSQNSSNSKRLAEVAADYGAPAHLIDDESEIDPSWLEGAERVGVTSGASAPEWLVER
                     VVERLRELGAPEVGQLQVAEEHVRFGMPYGVRDKDLRAAGV"
     gene            3715..4275
                     /locus_tag="VF097_12245"
     CDS             3715..4275
                     /locus_tag="VF097_12245"
                     /inference="COORDINATES: protein motif:HMM:NF015298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX6263607.1"
                     /translation="MKTPSHTGRRARGLLALGLVATLVLPLAGWAQSHPIDKFWRYGK
                     DVRHHKWREHPIGQRKHKRWHRSHPDATRWDHRRFHHRRLVHPNRSKHRFRIVAKQAG
                     QASYYSGSVGACGKRLIGLYAAHRSWPCGSKVSVKRGSRYVVVRILDRGPYTHGRVID
                     LSPAAFDRLGSLSAGVMDVRIFRLKK"
     gene            4315..4542
                     /locus_tag="VF097_12250"
     CDS             4315..4542
                     /locus_tag="VF097_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263608.1"
                     /translation="MARRPAQPLPRRRRGGRRQATLWQLFLGFLGVLATIAFMSVVSQ
                     PWRLAVLALGLAILGYMAAVYGYDSRDGKDW"
     gene            complement(4544..>4700)
                     /locus_tag="VF097_12255"
     CDS             complement(4544..>4700)
                     /locus_tag="VF097_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6263609.1"
                     /translation="WVTEQAIQVLGGYGYIKDFPVEKWYRDAKIYTLFEGTSEIQRLV
                     ISRALAG"
BASE COUNT          654 a         1700 c         1579 g          767 t
ORIGIN      
        1 cgagggggag ccaaccggtc gttctcccta cagccccggt cccacgcgct gttgagcggc
       61 ctttcaggac gggcgatact tcccggggca ctcccccagg agacgtcgag cgatgacggg
      121 tgacagatgc cgagggttcc cctgctctcg tactggcgcg ctgagcagcg cacgatccgc
      181 caggggctgg tggcgatgct cctggcctcc gtcgccatgc tcggcgccgg cgtcgcgctc
      241 ggcttcatca ccgacaccct ggagtcgctc cccggtctga tggtgatggt cccggcggcg
      301 atcgccgccc ggggcaacgt cttcggcgcg ctggcttccc gtctcggcac cgcgatccac
      361 gccggtctct tcgatacgac gcgccggcgc gagggcgtcc tgtaccagaa cgtcttcgct
      421 gcgaccgcgc tcacgctctc gatctccctg ctgatggccg tgctggcgag gaccctgtcc
      481 gccaccttcg gcgtcacgag catctcgatc atcgacttcg tcttgatctc gatcctgggc
      541 ggggtcgtgg cgagcgtcat cgtcctgggc ctcacgatcg tgctggcggt gaccgcgcag
      601 cgtcgcggct gggacctcga cagcgtggcc gccccgctca tcaccgcggc cggagatgtc
      661 ctcacggtgc cagcgatctt cctggccacc ttcgccgtgg ggttccgctg ggtgtccccg
      721 accctcaccg cgatcctgac gctggtcacg ctcgcagtga cgatccgcgc cgcgatcacc
      781 gatctgcgcg ccacccgccg catcgtccgc gagagccttc ccgtgctcac gcttgcggcc
      841 acgatcgaca tcctggctgg actgatgatc gagtcccagc tggagcagtt cgtgaccttc
      901 ccggtcctgc tgatcctgat cccgccgatc ctgggggacg ccggtgggct cggcggcatc
      961 ctcgcctcgc gcctcgcctc gaagctccat ctcggcgtga tcacgccacg aggcctccct
     1021 gaggccgccg cgctctcgga cctctcgatc gtcgcgctgt tcgcggtcgt cgtcttcccg
     1081 ctggtgggcg tggccgccca cgtggtggcg ggactcttcg ggatcgccag cccggggatg
     1141 ggcacggtcg tcggcgtcag cctgctcgcc ggcttgctgg ccacggcagc cgcgtcggtc
     1201 gtcgcctact acgcagcgat cgccagcttc cggatgggga tggacccgga cagccacggc
     1261 atcccgctga tcaccagctc gatggacctt gccggcgtgg tggcgctcat cctcgccatc
     1321 ttcgccttcg ggatcggata ggaccggccg tatactcggg aggcgccacg atggacgata
     1381 gccctagacg caacgtcaag gatctcctcg ctgaggcgaa ggacacctcg gagctcatgg
     1441 tcgacctggc ctacgcggcc gtcttcttca acgacgagga cctggccgac gaggtcgacg
     1501 ccctcgagga gctgatgcag ggctacatcc acgacctcaa ctggatctcg atgctggccg
     1561 cgcgcagccc cgaggacgcc gaggcgatgg cgggggtgtt ggcgatcgcc cgggcgatcg
     1621 agagcatcgg cgacgcggcc gccgacatcg cccgcgtggt gaccgtccgg ctggggatcc
     1681 ccacgacgct ccgggccgat ctccgccacg ccgacgagat cgtgggccgc gtcaaggtcc
     1741 gcgaggagtc cgaggtcgtg ggccgcagcc tccgcgacct cacgctcccc accgcgaccg
     1801 gcatgtgggt gatcgcgatc cgtcgcggca tggagtggga gaccgacccc gggcccgact
     1861 cggtggtcaa cgccggggac gtgcttctct accagggccc cgaggagggc gcgaacctgc
     1921 tccgcgagac ggccggcgcg ccgccgctgc cctcgcctcc cgacgaggag ccgctcgcgc
     1981 tctcggagct cgatcgcgcc gtcgatctcc tggtcgagat gaagaactcg gccgaggtcg
     2041 ccgtcggcct cgcatactcc tccttgctct tccgggagcc ggcgctggcc gccgaggtcg
     2101 ccgccctcga gacacgttcc gacacgctgc tcgacgagct ggagtcctgg gtccttcgcg
     2161 cctcccctga ggcccgcaac cccgacgagc tccgcgggct gctgcgcctg ggctacgcct
     2221 cggagatggt cttcgacgcc gcccgcgaga tgacccgcgc cgtggagagc ggggatgagc
     2281 tgcacccggt ggtccaggcg gctctggagg cgacggacga ggtggcggtg gagacggtcg
     2341 tggaagccgg ctctggagct gagggccaga ccctgaggga gctctcgctc gagaccgagg
     2401 cggggatgct ggccctcgcg atccagcggg gacgccggtg gatccaccgg ccccggcctc
     2461 gcttccagct cgaggccgga gaccgcgtca tcgccatcgg gcccgaggac gggatccccg
     2521 gtctcgaggc gctcacctcg gccccggccg agacgaacgc cggctgatca gacgccggcc
     2581 gcgcgcaggt ccttgtcccg gactccgtag ggcatcccga accggacgtg ctcctccgcc
     2641 acctggagct gccccacctc cggagcgccc agctcgcgaa gccgctccac cacccgctcc
     2701 accagccact cgggggcgga ggcgcccgac gtcaccccta ctcgctccgc cccttccagc
     2761 caggagggat cgatctccga ctcgtcgtcg atcaggtgcg cgggcgcgcc gtagtcggcc
     2821 gccacctcgg ccagccgctt cgagttcgag gagttctgcg acccgatgac gaggacgagg
     2881 tccacctccc tggccaatga cttcaccgcg tcctggcggt tctgcgtcgc gtagcagatg
     2941 tcgtcgcggg gcggccccac gatcgagggg aaccgctcgc gcaggacctc gatgatctcg
     3001 ttcgtctcgt ccaccgagag cgtcgtctgg gagaggtacg ccaccttcga tgggtccttg
     3061 acctctacgg ttcgggcctc gtccttcgat cccacgaggc ggatgtgctc gggggcctcc
     3121 ccgctcgtgc ccacgacctc ctcgtggccc tcgtggccga tcaggaggat cgtgtagccc
     3181 tccttggcga agcgccgggc ctcgaggtgc accttggtca ccagggggca ggtggcgtcg
     3241 atcacgttca acccccgggt cgccgcgttc cggtagacct ccggggccac accgtgagcc
     3301 gagagcacca ccgtggcccc ctccgggacc tcggtctcct cctcgacgaa gatcgcgccc
     3361 ttcgactcga ggtcccgaac gacgtggagg ttgtggacga tctgcttccg cacgtagatc
     3421 ggggcgccgt agagctgcag ggcccgctca accgtgtcga cggcccgctc cacgccggcg
     3481 cagtagcccc tgggggcggc gagggtgacc tctctgagca cgaccccatg ctaccccgat
     3541 ggccaccggg ccgacgccgc gctccctgtg gcgtttccgc gatcttcgtg taccctgctg
     3601 gcgcgcacga tcgtgcgacc gattcctctc cctcgcgggt ttcgtcagtt tgagcgcgcc
     3661 caggtcgggg tagggagggt cggttcaagc cgaacgagcg aacaggagga cggaatgaag
     3721 acgccctcgc ataccgggcg acgggcccgg gggctgctgg ccctgggact cgtcgccacc
     3781 ctggtccttc ccctcgccgg gtgggcgcag agccatccca tcgacaagtt ctggcgctac
     3841 gggaaggacg tgcgccacca caagtggcgg gaacacccga tcggtcagcg caagcacaag
     3901 cgctggcacc gcagccatcc cgacgccacc cgctgggacc atcgccgatt ccatcaccgt
     3961 cgcctcgtac acccgaaccg gagcaagcac cggttccgga tcgtggcgaa gcaggccgga
     4021 caggcttcct actacagcgg gagcgtcggc gcgtgcggca agcgcctcat cggcctctac
     4081 gccgcacatc ggtcgtggcc ctgcggctcg aaggtctccg tgaagcgcgg gagccgatac
     4141 gtggtcgtgc gcatcctgga ccgaggcccc tacacgcacg ggcgggtgat cgacctctct
     4201 ccggcggcct tcgatcggct ggggagcctc agcgccgggg tcatggacgt gaggatcttc
     4261 cggctgaaga agtagaagga acccgatggc tttaggatgg ggtcaggagg tcgcatggcg
     4321 aggcgtccgg cgcaacccct ccccaggagg cgacgaggtg gccgcaggca ggccaccctc
     4381 tggcagctct tcctcgggtt cctgggcgtg ctggccacga tcgccttcat gtcggtcgtg
     4441 agccagccct ggcgcctcgc cgtgctggcc ctcgggctgg ccatcctcgg atacatggcc
     4501 gccgtctacg gctacgactc ccgcgacggg aaggactggt agctcagccc gcgagggcgc
     4561 ggctgatgac gaggcgctgg atctcgctgg tcccctcgaa cagggtgtag atcttggcgt
     4621 cccggtacca cttctcgacg gggaagtcct tgatgtagcc gtagccgccg aggacctgga
     4681 tcgcctgctc ggtgacccag
//
LOCUS       DASUTZ010000285         3269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1294134, whole genome shotgun sequence.
ACCESSION   DASUTZ010000285 DASUTZ010000000
VERSION     DASUTZ010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294134"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /gene="glyA"
                     /locus_tag="VF097_12260"
     CDS             complement(<1..872)
                     /gene="glyA"
                     /locus_tag="VF097_12260"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006544704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX6263610.1"
                     /translation="MSDWIVDWEALERTDPEVAEAILGELERERTTLRLIASENYTSP
                     AVLAALANPMNDKYAEGYPGRRYYGGCRWVDVTETLAIERAKELFGAEHANVQPHAGA
                     QANMAAYFAFMEPGDTLLGMSLPHGGHLTHGSPVNFSGKWFEVIGYEVDKDSELIDLD
                     KVRDLAKEYRPKVIVAGATAYPRVIDFAGFREIADEVGAILMVDAAHFIGLVAGGAYP
                     SPVPHADVVTFTTHKTLRGPRGAMILSREEHAKEIDRAVFPMMQGGPLEHTIAAKAVA
                     LKEAAQPDFRDYAAR"
     gene            complement(869..1321)
                     /gene="rpiB"
                     /locus_tag="VF097_12265"
     CDS             complement(869..1321)
                     /gene="rpiB"
                     /locus_tag="VF097_12265"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX6263611.1"
                     /translation="MKIAIGSDHAGFALKEELKRHLKEMGHEVEDVGTFSEEPADYPA
                     FSAEVGRMVRDERVDRGIVLGGSGQGEQIAANKVRGVRAALCNDLFTAILSREHNDTN
                     VLAMGARIVAPPLAREILRVWLATGFDGGRHQRRVEQIAKIEEEEARR"
     gene            complement(1318..1905)
                     /locus_tag="VF097_12270"
     CDS             complement(1318..1905)
                     /locus_tag="VF097_12270"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HEX6263612.1"
                     /translation="MLRVLFVCTGNICRSPLAEAFLLERARGLPEGLLSVRSAGTWGR
                     EGHPAMPETIAIGVEHGLDVGDHAASPLTTDLIEGHDLLVGMARDHVAEIARMVPDSA
                     SRAFTLKELAHLLSSLEEPLPKPDVDAARARIAAADELRRARGGPADGDVSDPIGLGL
                     DVYRMVAGEIEAALDQVVEGLFGVRVSSGARKEGP"
     gene            1977..2612
                     /locus_tag="VF097_12275"
     CDS             1977..2612
                     /locus_tag="VF097_12275"
                     /inference="COORDINATES: protein motif:HMM:NF014997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEX6263613.1"
                     /translation="MASSKPRLRGRIHQVAFFVSFPAGASLIALADSPAARVVTAVYA
                     ASLTLLYGTSTLLHRLDWRPRVKTLMRKLDHSAIFLLIAGTYTAFAFLALQGTWRIAV
                     LSIVWGGAAAGIVLKGISVERFQVVTAVLYIVTGWVGIVAIAVFLPLLGAFRAALLVA
                     GGVLYTAGALVLLLRRPDPIPHVFGYHEIWHAMVVLASLCHYTVILLLVLG"
     gene            complement(2614..>3269)
                     /locus_tag="VF097_12280"
     CDS             complement(2614..>3269)
                     /locus_tag="VF097_12280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6263614.1"
                     /translation="GEAVASLEGADGGGAIVGTAAGEPVRCARLLIAAGPWTRSLAAQ
                     LGVDLPLTVERHIVGAYRRVEGVDVPAHGDLLGGYYLRPEGDDLHLVGSLHAAPQVDP
                     DDYDRRASREELERLAVPVIERVPGLEETSIHSGWASLYDVSPDWQPVIGEVAPGVFV
                     DAGTSGHGFKLAPALGRHVADLVAGEGVDPGLAAFHPSRFVKGEGLVAGYGEARILG"
BASE COUNT          433 a         1208 c         1117 g          511 t
ORIGIN      
        1 cgcgccgcgt agtcgcggaa gtcgggctgg gccgcctcct tcagggccac ggccttggcc
       61 gcgatcgtgt gctcgagcgg gccgccctgc atcatcggga agacggcccg gtcgatctcc
      121 ttggcgtgct cctcgcggct caggatcatg gcgccccgcg ggcctcgcag ggtcttgtgg
      181 gtggtgaagg tcacgacgtc ggcgtggggc acggggctgg ggtaggcacc gccggccacg
      241 aggccgatga agtgcgcggc gtccaccatg aggatcgcgc ccacctcgtc ggcgatctcg
      301 cggaagcccg cgaagtcgat cacgcgcggg taggcggtgg ccccggccac gatcaccttg
      361 ggccggtact ccttcgccag gtcccgcacc ttgtccaggt cgatcagctc cgagtccttg
      421 tccacctcgt agccgatcac ctcgaaccac ttgcccgaga agttcaccgg cgagccgtgc
      481 gtgaggtggc cgccgtgggg gaggctcatg ccgagcaggg tgtcccccgg ctccatgaac
      541 gcgaagtagg ccgccatgtt cgcctgagcc cctgcgtgag gctggacgtt ggcgtgctcc
      601 gctccgaaca gctccttggc ccgctcgatc gccagcgtct cggtgacgtc cacccaccga
      661 cagccgccgt agtaccggcg acccgggtac ccctccgcgt acttgtcgtt catcgggttg
      721 gccagcgcgg ccagcacagc cgggctcgtg tagttctcgg aggcgatcag gcggagggtg
      781 gtgcgctcgc gctccagctc gcccaggatg gcctcggcca cctcgggatc ggtccgctcg
      841 agggcctccc agtcgacgat ccagtcgctc atcgtctcgc ctcctcctcc tcgatcttcg
      901 cgatctgctc gacgcgccgc tggtggcgac cgccgtcgaa tccggtggcc agccataccc
      961 gcaggatctc gcgggccagc gggggggcga cgatcctggc gcccatcgcc agcacgttcg
     1021 tgtcgttgtg ctcccgggac aggatcgccg tgaagaggtc gttgcagagc gccgctcgca
     1081 ccccgcgcac cttgttggcg gcgatctgct ccccctggcc cgaccctccg aggacgatcc
     1141 ctcgatccac gcgctcgtcg cggaccatgc ggcccacctc ggcgctgaac gccgggtagt
     1201 cggcaggttc ctccgagaac gtgcccacgt cctcgacttc gtggcccatc tccttcaggt
     1261 gccgcttcag ctcctccttg agggcgaagc cggcgtggtc ggacccgatc gcgatcttca
     1321 cggcccctcc ttccgcgccc cggaggacac ccggaccccg aacagccctt cgacgacctg
     1381 gtccagcgcg gcctcgatct cgcctgccac catgcggtag acatccagcc cgaggccgat
     1441 cgggtcggac acgtcgccgt cggcgggacc ccctcgggcc cggcggagct cgtcggccgc
     1501 cgcgatcctc gcccgggccg catccacgtc cggcttcggg agaggctcct ccagggagct
     1561 cagcaggtgc gccagctcct tgagcgtgaa ggcccgcgag gccgaatccg ggaccatccg
     1621 ggcgatctcg gccacgtggt cacgggccat ccccacgagc aggtcgtgtc cctcgatcag
     1681 gtcggtggtc agggggctcg ccgcgtggtc cccgacgtcg aggccgtgct cgacgccgat
     1741 cgcgatcgtc tcgggcatcg cgggatggcc ctcccgcccc caggtgccgg ctgagcggac
     1801 cgacagcagg ccctccggga ggccgcgggc ccgttcgagc aggaacgcct cggccagggg
     1861 ggagcgacag atgttgccgg tgcagacgaa gaggacccgg agcacgaaag gagtgtagag
     1921 catcggccgc gggagccggc cggagcctcg agccgtcctg ctggtagcat cgcagaatgg
     1981 cctcgtcgaa gccgcggctc cgtggccgga tccatcaggt cgcgttcttc gtgtcgttcc
     2041 cggccggagc ctcgctcatt gctttggccg attcgccggc cgccagggtc gtcaccgcgg
     2101 tctacgcggc gagcctcacg ctcctctacg ggacgagcac gctcctccac cggctcgact
     2161 ggcgaccccg agtgaagacc ttgatgcgga agctcgacca ctcggcgatc ttcctgctga
     2221 tcgccgggac ctacaccgcg ttcgccttcc tcgcgctcca ggggacctgg aggatcgcgg
     2281 tcctctcgat cgtctggggc ggcgcggccg ccgggatcgt cctcaagggc atcagcgtgg
     2341 agcggttcca ggtcgtcacc gccgtcctgt acatcgtgac cggctgggtg ggcatcgtgg
     2401 ccatcgcggt cttcctccct ctgctgggag cgttccgagc cgccctgctg gtggcgggcg
     2461 gcgtcctcta caccgccggg gccctggtgc tcctcctgag gcgccccgac ccgatccccc
     2521 acgtgttcgg gtaccacgag atctggcacg ccatggtggt gctggcgagc ctgtgccact
     2581 acaccgtgat cctcctgctc gtcctgggct gagtcacccc aggatccggg cctcgccgta
     2641 gccggccacg agcccttctc ccttcacgaa gcgggaggga tggaaggccg ccagcccggg
     2701 gtcgaccccc tctccggcaa cgaggtcggc gacgtgccgg ccgagcgccg gagcgagctt
     2761 gaagccgtgg ccgctcgtcc ccgcgtccac gaagaccccc ggagcgacct cgccgatcac
     2821 gggctgccag tcggggctga cgtcgtagag gctggcccag cccgagtgga tcgaggtctc
     2881 ctcgagcccg gggacacgct cgatcaccgg gacggccaag cgctccagct cctcgcgcga
     2941 cgccctccgg tcgtagtcgt cgggatcgac ctgcggcgcc gcgtggagcg agccgacgag
     3001 gtggagatcg tcaccctcgg ggcggaggta gtagccgccg agcaggtcac cgtgcgccgg
     3061 cacgtccacg ccctcgacgc gccggtaggc gcccacgatg tgtcgctcga ccgtgagggg
     3121 gagatccacg ccgagctggg cggcgagcga gcgggtccag gggccggccg cgatcaggag
     3181 gcgagcgcac cggaccggtt cccccgcggc cgtcccgacg atcgcgccgc ccccgtccgc
     3241 tccctcgaga gaggccaccg cctcccccg
//
LOCUS       DASUTZ010000286         2477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_517839, whole genome shotgun sequence.
ACCESSION   DASUTZ010000286 DASUTZ010000000
VERSION     DASUTZ010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517839"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VF097_12285"
     CDS             complement(<1..411)
                     /locus_tag="VF097_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX6263615.1"
                     /translation="MSRFTDVAGLRVGHWTDAEAATGCTVVLCPEGTVASGEVRGGAP
                     GTRETDLLRPGMLVEHADAVLLTGGSAFGLAAADGVMRWLEERGRGFDGGVARVPIVP
                     AAVIFDLDAGDPAVRPGPDQGYAACEAASEEVPEG"
     gene            complement(408..1457)
                     /locus_tag="VF097_12290"
     CDS             complement(408..1457)
                     /locus_tag="VF097_12290"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6263616.1"
                     /translation="MSTARKASVGALAVAAGVAGAVLAGRTSVRRHRERPDPEWEEPF
                     GTLPQEDLGPVPSFDGTHLAVRAAGPRDAPAIVFSHGITLDMTTWYYQWRPLSARYRC
                     VLFDHRAHGRSENPPGGDYGLEALGKDLRVVLDRSVGSGPVVLVGHSMGGMGILSFAD
                     QFPEEFKDRVRGVVLADTAASDVLRELLGTLGASLERRLRPFTDRVLFDPRRAARIQH
                     VMDRWGRDLAFLIARTTNFGPDASAAQIDYLTRISAGARVEVWTQMLRGLMELDLARA
                     LGSITVPALVVVGDRDALTPKTSARAMREALPDGRAYVITRAGHVAMMEQHRIFNELL
                     EGFLDEVLIREQAAG"
     gene            complement(1454..>2477)
                     /gene="alr"
                     /locus_tag="VF097_12295"
     CDS             complement(1454..>2477)
                     /gene="alr"
                     /locus_tag="VF097_12295"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX6263617.1"
                     /translation="YMAVVKANGYGHGAVPIARAALEAGAEWLGVALVEEGVALREAG
                     ITARVLVLTEFPRGSEKEALAADLTPVLYTEEGIEGLAEAAETLGLRARVHVKVDTGM
                     HRVGLLPDLAVGFLERAAGRGLGIEGILTHFAKSEAPDDPATRQQLATFGKLLTTLSE
                     RGSCPPICHAANTAASMVIPESRLDLIRVGIGTFGIAPDPSLAGFADLRPVMSLRSRV
                     TLVKRVGRGHAVSYGFTYRLSRDATIVTVPVGYADGFARSLSNRARVLIRGQRYPVVG
                     TVTMDQLLVDCGDDPVEAGDEVVLWGRQGDEEILAEDVAAWWGTTGYEVVCAVSERVP
                     REYLHG"
BASE COUNT          346 a          957 c          870 g          304 t
ORIGIN      
        1 gccctcgggg acctcctccg aggcggcctc gcaggcggcg tacccctggt ccgggcccgg
       61 ccggacggcg gggtcgcccg cgtccaggtc gaagatcacg gccgcgggga cgatggggac
      121 gcgggcgacc ccgccgtcga atccccggcc ccgctcctcg agccagcgca tcacgccgtc
      181 ggccgccgcc agcccgaacg cgctgccccc cgtcaggagg accgcgtccg cgtgctccac
      241 cagcatgccg gggcggagga ggtcggtctc ccgggtcccc ggcgcccctc cccgcacctc
      301 gcccgacgcg accgtcccct ccgggcagag gacgacggta cagccggtgg cggcctcggc
      361 gtccgtccag tgacccacgc ggagcccggc cacgtcggtg aaacggctca cccggcggcc
      421 tgctcccgga tcaggacctc gtccaggaac ccctcgagca gctcattgaa gatccgatgc
      481 tgctccatca tcgccacgtg cccggcccgc gtgatcacgt aggcccgacc gtcgggcagg
      541 gcctcgcgca tcgcccgggc gctcgtcttc ggggtgaggg cgtcccggtc ccccaccacc
      601 acgagcgcgg gcacggtgat cgaccccagg gcccgggcca agtccagctc catcagcccc
      661 cgcagcatct gggtccacac ctcgacgcgc gcgccggcgc tgatgcgggt caggtagtcg
      721 atctgcgcgg cggaggcgtc gggcccgaaa ttcgtcgtgc gggcgatcag gaacgcgagg
      781 tctctccccc accgatccat gacgtgctgg atccgcgccg cccggcgggg atcgaagagc
      841 acccggtcgg tgaagggccg cagccgccgc tccagcgagg cgcccagggt tcccaggagc
      901 tcgcggagga cgtccgaggc ggccgtgtcg gccagcacca ctccccgcac gcgatccttg
      961 aactcctcgg ggaactggtc cgcgaacgag aggatcccca tcccgcccat gctgtgaccg
     1021 acgagcacca ccggccccga gcccacgctc cggtcgagga ccacgcggag atccttgccg
     1081 agagcctcca ggccgtagtc gccacccggc gggttctccg accgcccgtg cgccctgtgg
     1141 tcgaagagga cgcagcggta gcgggccgag agcggtcgcc actggtagta ccaggtcgtc
     1201 atgtcgaggg tgatgccgtg ggagaacacg atggccgggg cgtcgcgggg ccccgccgcc
     1261 cgcacggcga ggtgggtccc gtcgaaggag gggaccgggc ccaggtcctc ctggggcagg
     1321 gtcccgaagg gctcctccca ctcggggtcg gggcgctcgc ggtggcggcg aaccgacgtc
     1381 cggccggcga ggacggcccc cgcgaccccg gccgccaccg cgagcgcccc gaccgatgcc
     1441 ttcctggccg tgctcatccg tggaggtact cccgcggaac gcgctcgctg acggcgcaca
     1501 ccacctcgta tccggtggtc ccccaccacg cggcgacgtc ctcggccagg atctcctcgt
     1561 cgccctgccg gccccaaagg acgacctcgt ccccagcctc gaccgggtcg tccccgcagt
     1621 ccacgaggag ctggtccatc gtcaccgtgc ccaccaccgg gtagcgctgg ccccggatga
     1681 ggaccctggc ccggttggag agcgagcgcg cgaacccgtc cgcgtacccg acggggaccg
     1741 tgacgatggt ggcgtcccgg ctgagccggt aggtgaagcc gtacgacacg gcatgccctc
     1801 gtcccacccg cttcacgagc gtgacgcggg agcggagcga catcacggga cgcaggtcgg
     1861 cgaaccccgc gagcgacgga tcgggcgcga tgccgaaggt cccgatgccg acgcggatca
     1921 ggtccagccg ggattcgggg atgaccatcg acgcggcggt gttcgccgca tggcagatgg
     1981 gggggcacga gccgcgctcc gagagcgtcg tcaggagctt gccgaacgtg gcgagctgct
     2041 gccgggtggc cgggtcgtcg ggagcctccg acttcgcgaa gtgcgtgagg atgccctcga
     2101 tgccgaggcc ccgcccggcg gcccgctcca ggaacccgac cgcaaggtcg ggcagcagcc
     2161 ccacccggtg catcccggtg tcgaccttga cgtgcacgcg cgctcggagc ccgagggtct
     2221 cggccgcctc ggccagcccc tcgatcccct cctccgtgta gaggaccggg gtgaggtcgg
     2281 ccgccagggc ctccttctcg gaccctcgtg ggaactccgt gaggacgagc acccgggcgg
     2341 tgatgccggc ctcccggagc gcgaccccct cctcgaccag ggcgacgccg agccactcgg
     2401 cccccgcctc gagggccgcc cgggcgatcg ggaccgcgcc gtgcccatag ccgttcgcct
     2461 tcaccaccgc catgtac
//
LOCUS       DASUTZ010000287        23292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1776
            k141_1423787, whole genome shotgun sequence.
ACCESSION   DASUTZ010000287 DASUTZ010000000
VERSION     DASUTZ010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961109
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 23292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,449
            Genes (coding)                    :: 2,429
            CDSs (with protein)               :: 2,429
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 18 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23292
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423787"
                     /isolate="SMAG_U1776"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..506)
                     /locus_tag="VF097_12300"
     CDS             complement(21..506)
                     /locus_tag="VF097_12300"
                     /inference="COORDINATES: protein motif:HMM:NF044606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEX6263618.1"
                     /translation="MEIILRATAVYFFVYLLLRAMGKRELGEMSAFELVLLVMIGDLV
                     QQAVTQEDMSVTGAVLAVGTIAFWVMASSYLAFKSSRAEEVLESPPVVVVRDGVVQRE
                     ALRIERVSEADLMESARKHGVDDLRKVTLAVLESDGKFSFLQRISGPTQGEEEGHRAA
                     E"
     gene            complement(506..1039)
                     /locus_tag="VF097_12305"
     CDS             complement(506..1039)
                     /locus_tag="VF097_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263619.1"
                     /translation="MAPTEMKPLPTQPQEAVGLKEQRRHRIERRVGLGLVFAVLVLGA
                     ANFLGVRPATVSASGGSLELSVRYASVSRPGLATPWSVRVRSPGGFDGPVVLSTTSSY
                     FEMFDENGLDPDPQRATATGEALVWEFAPPEGEVLTVDYDARLEPAFHTGRSATTSVL
                     VDGDPVVSVTYRTVVMP"
     gene            complement(1121..3601)
                     /locus_tag="VF097_12310"
     CDS             complement(1121..3601)
                     /locus_tag="VF097_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263620.1"
                     /translation="MVPDVGVLFVHGIGLHEAGRTLRHMGGALTEYVRRRIGADPAVT
                     VEATQAGPSDDALMLTVRPSEGRTTTWRLQEARWDESFEEPTLGEVARWSLLVAPWIL
                     HREAFRWWARREVRGRARLLWRLAREGLGYVLRFVGFTLGAILLQVLLVLLVALAWSP
                     PVRRALDAILVRTVGDAHRFIYDPEALSIMSVLVARELHHLSGGCRKVIVVAHSQGAA
                     VARAAIDVGGAPQNLERVITLGAGLTKLHALQELRHRVAFLTYWLVLRLALLWLAVAT
                     FRGFGESIHTHLMLAVFLVAVFAPMRGVVRSERRTRELIARTSGLGSGWSWVDLWSTR
                     DLVPEGRLRASAHGRTPHVTSLQVDNAGSWISDHVTYLSNSGQVLPLIYGVAARASGW
                     TDPTLLRDVDWVWRHRSRRARTRGRGFLLAPFVCAALLLPIPTLLQVGDLGLPASALV
                     LASPLIWARLWRRWEMVCRRWEPQGCTVVAGWKRLLRPSFWWRLPPAAPPPGIDGHGE
                     HARWFALAIDGSHSAGPTLPDEAEKRHAAEPRALVLGAILSLLVIGAAAFPTLWELVR
                     DVGPSRGGTSHTALIANALMKPSVVVLAVASVLLPGVALDALGEIASSVARWHTRRVW
                     FGALACSLLVAGLGVSVGVILAITVRPIPALATGDCFDTHTANPHEIRSEQLHFDAMR
                     IPCREPHEAQLLQVHPLTEGSLPVLYDPTVLAAETCEGVVARGVLIDAAGLGALRLAA
                     YVPRSSMTLERGGGVYCFVAVAGCDQALVGHIARGVRIRARPAPVRGCGSGVAIATGG
                     SDMARADLSAIRTSYQLVDIGALTEAGA"
     gene            complement(3722..3805)
                     /locus_tag="VF097_12315"
     tRNA            complement(3722..3805)
                     /locus_tag="VF097_12315"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3769..3771),aa:Leu,seq:cag)
     gene            3899..4600
                     /locus_tag="VF097_12320"
     CDS             3899..4600
                     /locus_tag="VF097_12320"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HEX6263621.1"
                     /translation="MGILRDFEGRLERIVEGFFSKAFRSGLQPVELAKRILRQMDAGK
                     TVGVSGVLVPNRYVVVLSEEDRERLTGMESALSRELETVVVDGAREREFTLVARPEVV
                     FETAERLRRGDFRIQAEISEATGPPPADVGPRPGGGGPLLALVEGGDALREYPITSDR
                     MTIGRLAGSEIELHDPGASRKHAEIRRQGDTVTVVDLGSTNGTLVNDRTITDHVLRDG
                     DRITIGRTTLEFRSG"
     gene            4604..5053
                     /locus_tag="VF097_12325"
     CDS             4604..5053
                     /locus_tag="VF097_12325"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX6263622.1"
                     /translation="MPPFVLTVLKILLLLLLYFFLYRAVRSIARDLYPRTRERRREPR
                     PTRARRGRGRPGRVVVLGPEGKKVGTYRLEGAMQIGRDATCEIRPPDTYISQVHARLA
                     PRGDGWVVEDMGSTNGTYLNQRKVQVPTRIAPGDRIRLGKTVLEVRK"
     gene            5050..6153
                     /locus_tag="VF097_12330"
     CDS             5050..6153
                     /locus_tag="VF097_12330"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEX6263623.1"
                     /translation="MRVRVGATTDVGVVREQNEDAYLLRAPIYAVADGMGGHQGGEVA
                     SRLALETLDRRSREDHEVDLRAAVRDANRVVLDRASGDTELAGMGTTLTLLRIEGPRV
                     RLAHVGDSRAYLLRDGDLRQLTDDHTLVNKMVKEGKLTTSEAKIHPHRSILTRALGVD
                     GDVEVDVFTVDVDLGDRILLCSDGLTSIVSDEVIRGVLASQADPQAACDELVAMANRG
                     GGPDNITVVVLDFEEGEEEALPARAAAETPPPAPPPEGPRRRPSLRGLLLWVLLPLVI
                     LIGGFLLARAYVNSQWFVGVHEGSVALYRGIPAEVLGFELFTLVERTDLPAAEAAALQ
                     PYRELREGITADSEEDARDVIEQIEEDLEPPIP"
     gene            6150..7463
                     /locus_tag="VF097_12335"
     CDS             6150..7463
                     /locus_tag="VF097_12335"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX6263624.1"
                     /translation="MSTPTPELIARARRNTELGLLILAVLLAVGAYALVGLGLEGRVP
                     AGVAGYGVLLAAGYVGTHLVVRYLAPHADPVFLPAATLLTGLGFAVIWRLDQDLAVAQ
                     TSWLLFGLLLFAATLIVIRDHRQLDAYTYTIGLLGIALLLLPILPGVGQEVRGARLWV
                     DLGPLRFQPAEIGKVLVVIFLASYLNARKELLAVATRRLGPIALPEPKHLAPLLLAWG
                     LSLVILFLQKDLGSSLLFFGLFVVMLWVATARGAYLLLGLLLFAAGSFLAYQAFGHVQ
                     DRVTIWLNALDPQLIGDESFQIAQSLFALGTGGIAGTGLGEGHPDFIPDAHTDFVFSA
                     IGEELGLLGATVVLMLFVLLIARGFRAAIARSEGDGFGQLLAAGLATILALQTFVIIG
                     GVTRLIPLTGITLPFVSYGGSSLVANFVLLALLIRVSATASKEPG"
     gene            7460..8935
                     /locus_tag="VF097_12340"
     CDS             7460..8935
                     /locus_tag="VF097_12340"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX6263625.1"
                     /translation="MTRQIQILAAAFFILFGALFVNLNYLQVIAAEDLANHPANKRLL
                     IEEYEIDRGEILARDRRTVLARSRATDGELKYLRRYPEGELYAHVSGYHSFVYSRTEL
                     EQSYNEYLSARASELLPQRFVDDILGRDREGATVVTTIDPELQQVATDALGSFQGGVA
                     AVDPRTGEVLALVGLPTFDPNRLSSHDGREVRRAWNQLIDDPDKPMLSNANDEVFPPG
                     STFKVVTAAAALENGFGPESTWDNPPSLDLPQTTNNLDNFGGSHCLGGASQITLAQAL
                     QVSCNVTFGQVGLAVGAEALMEQARRFGFSEDVPFDIPFAEGQVPEPSAFDQDLPGLA
                     FSAIGQQDVRTNPLHMALVAGSIGNGGVMMRPRLVMEIRDPSGRVIERFDPEVYGTPL
                     SGGNARALTQMMVSVVSSGTATGAQIPGVSVAGKTGTAQTAEGQPPHAWFIAFAPAEQ
                     PSVAVAVVVLGRGNLSSEATGGAIAAPIAKAVMEAALEEGS"
     gene            8932..10380
                     /locus_tag="VF097_12345"
     CDS             8932..10380
                     /locus_tag="VF097_12345"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX6263626.1"
                     /translation="MTTVLADRYELEEPLGAGGMASVYRGTDRILDRTVAVKVLDRSL
                     GRDPEFVERFRREARAAAGLTHPGTVAVYDSGEDGGRRFIVMELIDGETLADMLAREA
                     PLDPKHAALLAADILEALAAAHDRGLVHRDVKPGNVLLTSEGEPKVTDFGIARAASSE
                     TLTVGSKVLGTAAYLSPEQARGRPVDARCDLYAMGCVLYEMLTAAPPFRGGSPISVAT
                     KQIHEDPRPLPSSVPPALAAVVMQALEKDPDDRFPDARSMAAAIRGAAGGAGETMVLE
                     EGMLETEAIPAPPAAPPRRWVLPALLGALLLAALIGGLVLAFGRDDPTAESRGGRDRG
                     GAAAQSSPAESPQEEAEPLRPREAFTALVGTVQDALAAGEIEEKLARDIVKRAEDAME
                     AYGEADLQKAQDKAGDAINALVAGAEQGEVTPERASEIEQRIRGLQAGFEAYPPREPE
                     EEDEGGSDDRGPDGEGPPGPDGEGPPGQNDNT"
     gene            10426..12108
                     /gene="pknB"
                     /locus_tag="VF097_12350"
     CDS             10426..12108
                     /gene="pknB"
                     /locus_tag="VF097_12350"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEX6263627.1"
                     /translation="MAKQRVLGNRYEIERVLGEGGMAKVFLGTDRVLGRTVAVKVLSP
                     QYAEDQQFVARFRREAQAAAALNHPNIVSVFDTGSTGNVHYIVMEYVEGRTLQDTLRE
                     ESRLTPERSIEIGEAVAKALSAAHAKGLVHRDIKPGNIMITREGQVKVMDFGIARATT
                     SDTVTQTAAVLGTAAYLSPEQAQGETVDHRSDIYSMGVVLYEMLTGRQPFTGDSPVSI
                     AYRHVTEEPIPPSRLNPDVRESLDAIVMKSMAKAPGDRYQSAQELIDDLRRASEGMPV
                     AAAVPLPGDTTQVIDRGGHTQVLTGPIEDEMEEEERGRRVGWIVFWTLLILAILGLAS
                     FFLVRALLSGPEPVTVPDVVGFNRVAAVQTLEEAGLEVEVVRRARDDVPADQVFRQDP
                     EEGTEVDEGSVVTIFVSRGARMVEVPSVVGLSQEAAQQAIEDAGLTLGFVGQEPSDQD
                     PGIVISQDPQPETEVEEGSAVNIVVSSGPDTVAVPSVVCDPPEDAQSEIEGRDLVYQE
                     EAEEFSDCPSGTVARQDPEAGAEVAPGSTVRVWISLGPSPEPSPTDSPTDGA"
     gene            12098..13702
                     /locus_tag="VF097_12355"
     CDS             12098..13702
                     /locus_tag="VF097_12355"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX6263628.1"
                     /translation="MAPETSWVEETFTDEERERLAPFVTSLEGPVFALINLPEVTKGA
                     LFARYSRSSKSVRRLLLDEFLEEGESGAAAEEAGVSRAERLYENVFFEYGDDSVAQLG
                     GVHLACEQSSQILAKVLEWGRLAAYLEQSTRYVPYDDRPGGRYRYAIPPEVEAAGLSA
                     RYSEVLDRAFDTYARWLEPMQDLYRARHPQDPGDPDFVYRSTIRAKACDTLRGLLPAA
                     TRTNVGIYATGQAYEQLLLRMRAHPYAEVRAYADLMLTELRKVIPAFLRRVDLPDRGG
                     AWSDYLARTREETAKVAEELAGDLRAPPRPEATLTDFDPEGEVKVVAAAMYASTGLPD
                     DVLLERARAMSTEQRDRVLAAYVGDRPNRRHKPGRAFERTGYRFDVLCDYGAFRDLQR
                     HRMLTIEWQELTTAHGFERPDDVEEAGARPDWDRVMEESAALEGELRAAGLGGVAQYS
                     VAMAYRIRFVMQMNAREAMHLIELRSSPQGHPAYRRVAHQMHRLIRDEAGHGAIAGSM
                     RHLDLSEVDLERLEAERRAASRRGSE"
     gene            13699..13821
                     /locus_tag="VF097_12360"
     CDS             13699..13821
                     /locus_tag="VF097_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263629.1"
                     /translation="MTKQDWLFLALGTLAIASLLTSIGTWMVLWDRLRSRRYES"
     gene            complement(13809..15569)
                     /locus_tag="VF097_12365"
     CDS             complement(13809..15569)
                     /locus_tag="VF097_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX6263630.1"
                     /translation="MANDRTQRPRPWLDHYAEGVPQTLEPYPEMSAFEMLERTVQRFP
                     DSPAVAFPVAPMSRTLTYRQLLDEVEQMARALASLGVKRGDRVGLLLPNSPHFVVAHF
                     AIQRLGAITVGNNPLYTKRELVHQLNDAGVDVLIALDLLYPLVEAARTEVAFRKVIVG
                     KVGDYLGFPVNMLAPLKQRREAKHEGRRWPPVPQDADVTWWADLMKGSYPDVPPAQVD
                     PKEDVAALVYTGGTTGLSKGAMLTHFNIVANAAQTAAWFTGIEEGHEGIVAVLPFFHS
                     YGLGAIMGVGIYKGLKLVLLPRFELELVLKAVEKERPTLFPGVPRIFIAINESPETAK
                     HDISSIRSCFSGAAPLPVAVAERFEQITGGRLVEGYGLTETSPVTHINPLDGSHRFGT
                     IGLPIPDTDCKLVDIDDPDREVDGDDEGELCIAGPQVMKGYWNRPEETEEMIRVHPDG
                     IRWLHTGDIAKMDPDGYFRIVDRKKDIIIVSGFNVYPTDVEQVLYRHPKVQKVSVVGI
                     PDEKTGEAVKAFVILKEGQEATEDEIKTWARDPELGLTGYRAPRHVEFRDELPETMVG
                     KVLRRKLQEEEKEKASAQDS"
     gene            15676..18015
                     /locus_tag="VF097_12370"
     CDS             15676..18015
                     /locus_tag="VF097_12370"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX6263631.1"
                     /translation="MDDWIEALRGAARAALPSVEGEVRLPGLKAAVEIRRDRWGVPHI
                     YADHLTDLWTAQSYVMASERLFQIELAYRLGLGRLSELFGELTLSMDRFVRTLGWNRA
                     AADHAATWDDLSLEMTAAGAAGALAWLEAMPARPVEYVVLGVDPWLPEPEEAALASAG
                     AAMYVAWSLSRAWDNDLLRAEIAERFGVEAMRALFPDALPEPEAVRAAKEATDPRLAM
                     LRDAILAPSGEGSNAWVVAGSRSATGAPLLANDPHLVVQAPSAWFEAHLVGPGVDVAG
                     VTFPFTTGIVIGHNDRIAWGFTNSEADVQDLYLERLSEDGASTQHLDAWEPVTMVREE
                     IGVRGRDEPEVLEVRETRHGPLVGSYMIGIASPRVIQGRIRRPYALRWVGAEGTIEPS
                     TIHRLDTATSWEQFRAAVARWHCPGQNMVYADVEGNIGYQLTGRYPARRTGDGSLPAP
                     GWTEEHEWDGWIPFDELPRAFNPEAGFVVTANNRPDGEGGPFLGVDFLPPYRARRIAQ
                     LVTARPRHDRESFAAIQTDTRSLLAPEILRHLLAIEPGDERQTEALGLMAEWDWDLGR
                     DSAPAAICESWCTHLAARILRPRLGRELFEHYFARRQWTNGFQYRVLPELLAYPTGGW
                     FGEDGAAGRDKEVGAALDEALTELTDRLGEHMADWRWGAVHRARFAGRLAAVPGLGEL
                     FTVGEVELGGDEQTVAQAHFEPGAPYEAVVVPSWRQIVDLGDLDSSVGVLTLGQSGNP
                     ESPHYRDQLPLWESGQHHPMPFTRAAVERATTTTLRVIP"
     gene            complement(18012..18725)
                     /locus_tag="VF097_12375"
     CDS             complement(18012..18725)
                     /locus_tag="VF097_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEX6263632.1"
                     /translation="MTEEIAATDAYARETPATVLGSGEDGVVLDRTVFYPRGGGQPGD
                     TGLLRWDGGLVRVVDTVRRDGRVLHVAEGDAPAPGTVVTAAIDWERRHRLMRIHTALH
                     ALSGLIWRDYEAKVTGGNMDVGTARMDFELEQMSAEFGREVEEKLNAALAEDRPVHVR
                     FLPRAEALADPDLIRTKVNLIPESVDPIRVIDIEGIDRQADGGTHVRSTGEVGRVLVT
                     KTESKGKAFKRVRIALDEG"
     gene            complement(18735..18887)
                     /locus_tag="VF097_12380"
     CDS             complement(18735..18887)
                     /locus_tag="VF097_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263633.1"
                     /translation="MIGFFRRLSPRNAVLAVLYWIVLVALAVILVFALFYLLDVSRFV
                     PGGGMF"
     gene            complement(18884..19534)
                     /locus_tag="VF097_12385"
     CDS             complement(18884..19534)
                     /locus_tag="VF097_12385"
                     /inference="COORDINATES: protein motif:HMM:NF033747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HEX6263634.1"
                     /translation="MTVEAPPRSGRRRRGLGSRLVRGAGWTLITAGTLVALFVVYELF
                     VTDLITGRHQDALRQDLGRRLQVASPNQDLRPVPGRGVAILRIPRIDLDAVVVEGVGL
                     EDLKRGPGHYPRTPLPGDGGNVGIAGHRTTYGKPFWSLNELAPGDEIFLRTNRGEFAY
                     EVLWTRVVLPSQSEVLDRTPRPSLTLTTCEPRFSAARRLIVRAVQVDPPSDLEAPG"
     gene            complement(19531..20238)
                     /locus_tag="VF097_12390"
     CDS             complement(19531..20238)
                     /locus_tag="VF097_12390"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEX6263635.1"
                     /translation="MDRPGSRFAMPLAAGVLGFLAVLAAAQPQAVPGRESSRLEIVDL
                     IEQQDGRVRELREEVAELEARIREAGGGETSAAIGELRAEAEALAVGAGATPLRGPGI
                     TVVLDDSSASRSGTGDPNDLIVHERDIQTVVNALWAAGAEAVAINGERLSSLSAVRCA
                     GNTLLLHGVVHSPPYEIRAIGDPDALERSVEEGPGMPRLHEAVRAFGLGLEIERGSLV
                     VPVGRGVRELATAAPAA"
     gene            20278..20511
                     /locus_tag="VF097_12395"
     CDS             20278..20511
                     /locus_tag="VF097_12395"
                     /inference="COORDINATES: protein motif:HMM:NF018487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HEX6263636.1"
                     /translation="MPKSKSKRRSYQPPKPPKPKPSPTWFGILVLTLLFVGVLMIVLN
                     YLGFLLPGSPNNTYLWVGLGLIAVGFALATQWR"
     gene            20591..20845
                     /locus_tag="VF097_12400"
     CDS             20591..20845
                     /locus_tag="VF097_12400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX6263637.1"
                     /translation="MPVYEFRCEDCGAALELVRPMGRTEPPGSCPACGGSRMRRRWSR
                     VGVRFEGWGFSRTDALLPEDRRARRDFKQLKRRADEIAES"
     gene            complement(20842..21093)
                     /locus_tag="VF097_12405"
     CDS             complement(20842..21093)
                     /locus_tag="VF097_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263638.1"
                     /translation="MRFAFAVLLAVGVAGLGIWILSAIGRNRDLVREAPVEDVEALDV
                     YFVCGECGTEFRVEKLGELQVPRHCGETMRVEQRPAPLR"
     gene            complement(21098..22279)
                     /locus_tag="VF097_12410"
     CDS             complement(21098..22279)
                     /locus_tag="VF097_12410"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013987393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX6263639.1"
                     /translation="MREAVIVEAVRSPVGRRGGKLSGSHPVDLLAEVLSAAVERTGVD
                     PARVDDNITGCVSQTGEQAINIGRNAWLAAGLPEEVPSVTVDRQCGSSQQAAHFAAQG
                     VMAGAYDLAIASGIEHMTRVPMGVTMEQGPGFPFSQRLLDRYDGRLVPQGVSAEIIAE
                     RWNLSRGELDEFSARSHELAAKAIKDGRFQDEIHPIELGTERETELFEHDEGVRFEVD
                     LEKMATLPTPFKEDGVVTAGNSSQISDGAAAVVIASREKADELGLRPRARFQAFALSG
                     VDPITMLTGPIEATRKVLASGGLSLEDVDVLEINEAFASVVLAWKRELEIPDDWFEAH
                     VNPNGGAIALGHPLGASGGRLMTTMLHELERREGRYGLQVMCEGGGLANATIIERLVV
                     D"
     gene            22414..23265
                     /locus_tag="VF097_12415"
     CDS             22414..23265
                     /locus_tag="VF097_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263640.1"
                     /translation="MRTVLGRGSATGAALAAVAVALSLTTTVLATPTTINDVDLTAGG
                     GAVFDPEHETYGCVFDEDTRYYSPIGDGSIGIRTDGFDGALVLWVGDNAFTDADGVGN
                     LVGQQLTVGPTKVSGVKVTRIERALPGSRTLRSLIKLTNPSKKILRRSITLDTNLGSD
                     GSTALELSSSGDIRFDSRDRWGITSEQPGSDPVVTMVNYGKGRVVRPQFQIGPVAGDE
                     CILTSFRITLGPKQTRYMMFFVELSEDDLDAASSARKFNRKRLSSALLAGLGSEVRQR
                     ILDWDLT"
BASE COUNT         3499 a         8358 c         8077 g         3358 t
ORIGIN      
        1 gacatcgcgc gcggcccccc tcactccgcc gcccggtggc cctcctcctc gccctgggtg
       61 ggccccgata tccgctgcag gaacgagaac ttcccgtccg attccagcac cgcgagcgtc
      121 accttccgca gatcgtccac cccgtgctta cgggccgact ccatcaggtc cgcctcgctc
      181 acccgttcga tcctgagggc ctctcgctgg acgacgccgt cccgcaccac caccactggg
      241 gggctctcca gcacctcctc ggcccgggag gacttgaagg caaggtacga cgatgccatg
      301 acccagaacg cgatcgtccc caccgccagc accgcccccg tcactgacat gtcctcctgg
      361 gtcactgcct gctgcacgag gtccccgatc atcaccagca ggaccagctc gaaagcgctc
      421 atctcgccca gctcccgctt ccccatggcc cgaaggagca ggtacacgaa gaagtagacc
      481 gccgtggcgc gaaggatgat ctccatcagg gcatcaccac cgttcggtag gtcacggaga
      541 cgaccgggtc gccgtccacg aggaccgagg tcgtggccga ccggccggtg tggaaggcgg
      601 gttccaggcg ggcgtcgtag tccaccgtga gcacctcacc ctcggggggc gcgaactccc
      661 acacgagcgc ctcaccggtc gccgtcgctc gctggggatc cgggtcgagc ccgttctcat
      721 cgaacatctc gaagtagctc gaggtcgtcg acaggaccac ggggccgtcg aaccccccgg
      781 ggctgcgaac ccggacgctc cagggcgtgg ccagacccgg cctcgacacc gacgcgtatc
      841 tcacgctcag ctccaggctt ccccccgagg ccgagacggt cgctgggcgg acgccgagga
      901 agttggccgc gcccagcacg agcaccgcga agaccagccc caggcccacc ctccgctcga
      961 tccgatgcct ccgctgctcc ttcagaccca cggcctcctg aggctgcgtg ggaagcggct
     1021 tcatctccgt aggcgccatc tccacctcct tgtcggggac gattcttccg ccaagcggac
     1081 cgaggacaaa ccactcgggt ccggtcccga ggaggggtcc tcaggcaccg gcctccgtga
     1141 gcgctccgat gtcgaccagc tgataggacg tgcggatggc cgacaagtcc gcccgagcca
     1201 tgtcgctccc tccggttgcg atcgcgaccc ctgagccgca gcccctgacg ggcgccgggc
     1261 gagctcggat gcggacgccg cgcgcgatgt ggcccacgag cgcctgatcg cacccggcca
     1321 ccgcgacgaa gcaatacacg ccccctcccc tctccagtgt catcgaggag cgcgggacgt
     1381 acgcggccag cctgagggcg cccaagccgg ctgcgtcgat gagcactcct ctcgcgacga
     1441 ctccctcgca cgtctcggcc gcgagcaccg tcgggtcgta caagaccggg agtgagccct
     1501 cggtgagcgg atgcacctgc agcagttggg cctcatgggg ctctcggcac gggatcctca
     1561 tcgcatcgaa gtggagttgc tccgagcgga tctcgtgcgg attcgccgta tgagtatcga
     1621 agcagtcccc cgtggccaga gcgggaatgg gacgcaccgt gatcgccagg atcacgccca
     1681 cgctcacccc caggcctgcc accaagaggg agcaggcgag agcaccgaac cacacgcggc
     1741 gcgtgtgcca acgggcaacg ctcgaggcga tctcccccag cgcatcgagg gcgacccccg
     1801 gcagcagcac cgaggctacg gcgagcacga cgaccgaggg cttcatcagg gcgttcgcga
     1861 tgagcgccgt gtgggaggtc cccccacgac tggggccgac gtctcgcacg agttcccaga
     1921 gcgtcgggaa cgccgcggcg ccgatcacca ggagagacag gatcgctccg aggacgagcg
     1981 cgcggggctc ggcggcgtgc ctcttctctg cctcgtctgg aagtgtgggc ccggcggaat
     2041 gtgatccatc gatcgccaga gcgaaccacc gggcatgctc gccatgtcca tcgatgccgg
     2101 gagggggcgc ggccggaggg aggcgccacc agaagctcgg gcgcagcagg cgcttccagc
     2161 ccgcgactac ggtgcacccc tgtggctccc atcgtcggca gaccatctcc caccgacgcc
     2221 acaggcgggc ccagatcagg gggcttgcga gtacgagcgc gctcgccggc aaccccaggt
     2281 ctcccacctg gaggagagtg gggatcggga ggaggagcgc tgcacacacg aagggcgcga
     2341 gcaggaaccc tcgccctcga gtccgtgctc gtcgcgagcg gtgtcgccag acccagtcca
     2401 cgtctcgcag gagcgtcggg tcggtccacc ccgacgccct ggccgcaaca ccgtagatca
     2461 gcgggaggac ctgacccgag ttagacaagt acgtcacgtg atcgctgatc caagatccgg
     2521 cgttatccac ctggagactc gtgacgtggg gtgtccggcc gtgcgcggac gcgcgcagcc
     2581 tcccctccgg cacgaggtcg cgcgtcgacc aaagatcgac ccagctccat ccggacccca
     2641 accccgacgt ccgcgcgatc agctcccggg tccgacgctc gctgcgaacg acgccccgca
     2701 tgggggcgaa cacggcgacc aggaaaaccg cgagcatcag atgcgtgtgg atcgattccc
     2761 cgaacccccg gaacgtggcg accgcgagcc agaggagcgc gaggcgcaac accagccagt
     2821 acgtgaggaa ggcgacccgg tgacggagtt cctgcaaggc gtggagcttc gtcagcccgg
     2881 cccccagcgt gatcaccctt tccaggttct gaggcgctcc cccgacatcg atcgcggccc
     2941 gcgccaccgc cgcgccctgg gagtgggcca ccacgatcac cttccggcac cctcccgata
     3001 ggtggtgcag ctcccgcgcc acgagcacgg acatgatgga gagagcctcc gggtcgtaga
     3061 tgaagcgatg agcgtcgccg accgtgcgca cgaggatcgc gtcgagggcg cgacgaaccg
     3121 gaggcgacca ggccagggcg accagcagaa ctaggagcac ctgcagcagg atcgccccca
     3181 gggtgaagcc cacgaagcga aggacatagc ccaggccctc tcgggctagg cgccagagga
     3241 gcctcgctcg ccctctcacc tcgcgccgcg cccaccacct gaacgcctca cgatggagga
     3301 tccacggcgc cacaaggagg ctccagcgcg cgacctcccc gagcgtgggc tcctcgaacg
     3361 actcgtccca acgcgcttct tggagccgcc acgtcgtcgt ccggccttcc gagggtcgaa
     3421 cggtgagcat gagcgcatcg tcggacgggc ccgcttgggt ggcctcaacg gtgaccgcgg
     3481 gatctgcccc gatccgcctc cggacgtatt cggtgagcgc gccccccatg tgacgaagcg
     3541 tcctcccggc ctcatggagg ccgatgccgt gcacgaagag gacgcccacg tcggggacca
     3601 cgctcgagct cgtggaagag gctcccgcct ccgatgcacc catctggacc ttcggcgggg
     3661 ccatggtgcg ctcgcacgcc tgccttgtcc agagccgctt ggtcgggatc agccgctgcg
     3721 gtgggcgagg ggggagtcga acccccatgg gcttgcgccc acaggatcct gaatcctgcg
     3781 cgtctaccag ttccgccacc cgcccgggtg gtcccctggg gacggaccca tggtactcga
     3841 cggggcggcc ccccgggtcg ggtgtaggga cagggcgagc gcggtaccct ttgcccggat
     3901 ggggatcctg cgagacttcg agggcaggct ggaaaggatc gtggagggct tcttctcgaa
     3961 ggccttccga tcgggcctcc agccggtcga gctggccaag cgcatccttc gccagatgga
     4021 cgcggggaag acggtcggcg tcagcggcgt gctcgtcccc aaccgctacg tggtcgtcct
     4081 ctccgaggag gaccgggagc gcctcaccgg gatggagtcg gcgctgtccc gtgagctaga
     4141 gaccgtggtt gtggacggcg ccagggagcg cgagttcacg ctcgtggccc ggcccgaggt
     4201 cgtcttcgag accgcagagc gactgaggcg gggggacttc cgcatccagg ccgagatctc
     4261 ggaggcgacc ggcccgcccc cggccgacgt gggcccccgg cccgggggag gcggccccct
     4321 gctcgcgctg gtcgaggggg gcgacgcgct ccgcgagtac ccgatcacga gcgaccggat
     4381 gacgatcggg cgcctcgcgg ggtccgagat cgagctgcac gacccggggg cgagcaggaa
     4441 gcacgccgag atccggcgtc agggcgacac ggtcaccgtc gtcgacctcg gatccacgaa
     4501 cggcaccctt gtcaacgacc ggaccatcac cgaccacgtc ctccgcgacg gcgaccggat
     4561 cacgatcggt cgcacgacgc tggagttcag gagcgggtag cccgtgcccc cgttcgttct
     4621 cacggtcctc aagatcctcc tcctgctcct cctctacttc ttcctctacc gggccgtgcg
     4681 gtcgatcgcc cgcgacctct acccgcggac gcgggagcgg cgccgcgagc cgcgcccgac
     4741 ccgggcccgc aggggacggg ggcggccggg ccgcgtggtc gtgctggggc cggagggcaa
     4801 gaaggtgggg acctaccggc tcgagggagc gatgcagatc gggagggacg ccacctgcga
     4861 gatccgcccg cccgacacgt acatctccca ggtccacgcg cgtttggctc cgcgcggcga
     4921 cggctgggtg gtcgaggaca tgggctccac caacggcacc tacctcaacc agcggaaggt
     4981 gcaggtgccg acccgcatcg cccccggcga tcgtatccgc ttggggaaga cggtcctgga
     5041 ggtgaggaag tgagggtccg ggtgggcgcc accaccgacg tcggggtcgt ccgggagcag
     5101 aacgaggacg cctacctgct ccgggcaccg atctacgcgg tcgccgacgg catgggcggg
     5161 catcagggcg gggaggtggc gtcgcgcctc gccctggaga ccctcgaccg gaggagccgc
     5221 gaggaccacg aggtcgacct tcgagccgcg gtcagggacg ccaaccgggt cgtcctcgat
     5281 cgggcatcgg gcgacaccga gctcgcgggg atggggacca ccctcacgct cctcaggatc
     5341 gagggccccc gcgtgcgcct ggcccacgtc ggcgactcgc gcgcgtacct gctccgggac
     5401 ggagacctcc gacagctgac cgacgaccac accctcgtca acaagatggt caaggagggg
     5461 aagctcacca cgagcgaggc caagatccac ccccatcgca gcatcctcac cagggcgctc
     5521 ggggtcgacg gcgacgtcga ggtggacgtc ttcacggtgg atgtggacct cggcgaccgc
     5581 atcctgctct gcagcgacgg gctcacgtcg atcgtgagcg acgaggtgat ccgcggggtc
     5641 ctcgcctcgc aggccgaccc tcaggccgca tgcgacgagc tcgttgccat ggcgaaccgg
     5701 ggaggcgggc ccgacaacat caccgtggtg gtcctggact tcgaggaggg cgaggaggag
     5761 gcgctccccg cccgagccgc ggcggagacg ccgccgcccg ctccgcctcc cgagggtcct
     5821 cgccgccggc cctccctccg aggactcctg ctgtgggttc tcctgcccct ggtgatcctg
     5881 atcggcggct tcctgctggc ccgcgcctac gtcaactcgc agtggttcgt gggggtccac
     5941 gagggcagcg tcgccctgta ccggggcatc cccgcggagg tcctgggctt cgagctcttc
     6001 acgctcgtgg agcggaccga ccttcctgcg gccgaggccg ccgcgctcca gccgtaccgg
     6061 gagctccggg aggggatcac cgccgacagc gaggaggacg cccgcgacgt gatcgagcag
     6121 atcgaggagg acctggagcc gccgatccca tgagcacgcc cacccccgag ctcatcgccc
     6181 gggcccgccg caacaccgag ctgggcctgc tcatcctcgc cgtcctgctg gcggtggggg
     6241 cctacgcgct cgtgggcctg gggctggagg gccgggtccc ggccggcgtg gcgggctacg
     6301 gcgttctcct cgcggccgga tacgtgggga cgcatctcgt ggtccggtac ctcgcccccc
     6361 acgccgatcc ggtgttcctc ccggcggcca cgctcctcac cgggctgggg ttcgccgtga
     6421 tctggaggct cgaccaggac ctcgccgtgg cccagacctc gtggctgctg ttcgggctcc
     6481 tgctcttcgc ggccaccctg atcgtgatcc gcgaccaccg gcagctcgac gcctacacgt
     6541 acacgatcgg cctgctcggc atcgcgctcc tgctccttcc aatcctccca ggggtgggcc
     6601 aggaggttcg gggggcgcgg ctgtgggtgg acctgggccc tctccggttc cagcccgcgg
     6661 agatcgggaa ggtgctggtg gtgatcttcc tcgcctcgta cctcaacgcg aggaaggagc
     6721 tgctcgcggt ggccacccgg cgcctggggc ctatcgccct cccggagccg aagcacctcg
     6781 ctccgctgct gctggcctgg gggctgtcgc tcgtgatcct gttcctgcag aaggatctgg
     6841 gttcgtcgct gctgttcttc gggctgttcg tggtgatgtt gtgggtggcc acggcacgag
     6901 gggcctacct cctgctgggg ctgctgctgt tcgccgcggg cagcttcctc gcctatcagg
     6961 cgttcgggca cgtccaggac cgggtcacga tctggctgaa cgcgctcgac ccgcagctca
     7021 tcggcgacga gtcgttccag atcgcccagt cgctgttcgc gctggggacc ggcgggatcg
     7081 cagggacggg cctgggggag gggcatcccg acttcatccc cgacgcccac accgacttcg
     7141 tgttctcggc gatcggggag gagctcggcc tgctcggcgc cacggtcgtg ctgatgctct
     7201 tcgtcctgct gatcgcgcgc gggttccgcg ctgcgatcgc caggagcgag ggggacggct
     7261 tcggtcagct gctcgccgcc ggcctcgcca cgatcctcgc gctccagacc ttcgtgatca
     7321 tcggaggagt gacccgactg atcccgctga cgggcatcac cctgccgttc gtctcctacg
     7381 gagggtcgag cctcgtggcg aacttcgtcc tgctcgccct cctcatccgc gtctcagcaa
     7441 cggcgtcgaa ggagcccggg tgacccgtca gatccagatc ttggccgcgg cgttcttcat
     7501 ccttttcggc gcgctgttcg tgaacctcaa ctacctgcag gtgatcgcgg cggaggatct
     7561 cgccaaccac ccggccaaca agcggctcct gatcgaggaa tacgagatcg accggggcga
     7621 gatcctggcc agggaccgcc gcacggtgct ggccaggtcc agggcgaccg acggcgagct
     7681 gaagtacctg cgccgctacc cagaaggcga gctgtacgcg cacgtaagcg ggtatcactc
     7741 cttcgtgtac agcaggaccg agctcgagca gtcatataac gaatacctgt ccgcccgcgc
     7801 gtcggagctg ctgccccagc gcttcgtgga cgacatcctg ggacgcgatc gggagggggc
     7861 cacggtcgtg accacgatcg accccgagct gcagcaggtg gccacggacg ccctggggtc
     7921 gttccagggc ggggtggcgg ccgtcgaccc gcggaccggc gaggtgctgg ccctggtggg
     7981 gctgcccacg ttcgacccca accgcctctc gtcgcacgac ggccgggagg tccgacgggc
     8041 ctggaaccag ctcatcgacg accccgacaa gccgatgctc tcgaacgcga acgacgaggt
     8101 cttcccgccc ggctccacct tcaaggtcgt gaccgcggcc gcggcgctgg agaacggctt
     8161 cggtcccgaa tccacgtggg acaatccgcc gagcctggat ctgccccaga ccacgaacaa
     8221 cctcgacaac ttcggcggat cccactgcct gggcggagcc tcgcagatca cgctggcgca
     8281 ggcgctccag gtctcgtgca acgtgacctt cgggcaggtg ggcctggcgg tgggcgccga
     8341 ggcgctcatg gagcaggcga ggcgcttcgg gttctccgag gacgtcccgt tcgacatccc
     8401 cttcgccgag ggccaggttc ccgagccgtc ggccttcgac caggacctcc cggggctggc
     8461 cttctcggcg atcggccagc aggacgtcag gacgaacccg ctccacatgg cgctggtggc
     8521 ggggtcgatc gggaacggcg gcgtgatgat gcgcccgcgc ctcgtcatgg agatccgcga
     8581 cccctccggg agggtgatcg agcggttcga tccggaggtc tacggcacgc cgctctcggg
     8641 cgggaatgcg cgggcgctga cccagatgat ggtgtccgtg gtctcctcgg gcaccgccac
     8701 cggggcgcag atccccggcg tgagcgtcgc gggcaagacc gggacggccc agacggccga
     8761 gggtcagccg cctcacgcct ggttcatcgc gttcgctccc gccgagcagc ccagcgtggc
     8821 cgtggccgtg gtcgtcctcg gcaggggcaa cctcagcagc gaggcgaccg gcggggcgat
     8881 cgcggccccg atcgcgaagg cggtcatgga ggccgcgctt gaggagggct catgacgacg
     8941 gtcctggcgg atcgctatga gctcgaggag ccgctgggcg cgggcggcat ggccagcgtg
     9001 taccggggta ccgaccgcat cctggaccgg accgtggccg tcaaggtcct ggaccggtcg
     9061 ctgggacgcg atcccgagtt cgttgagcgg ttccgtcggg aggcgcgcgc ggctgcgggg
     9121 ctgacccacc ccggaacggt ggccgtctac gacagcggag aggacggcgg tcgccggttc
     9181 atcgtgatgg agctgatcga tggggagacg ctggccgata tgctggcccg cgaggcgccc
     9241 ctcgatccga agcacgcggc gctcctggcc gccgacatcc tggaggccct ggcggccgcc
     9301 cacgaccggg gactggtgca tcgagacgtc aagccgggga acgtgctcct gacgagcgag
     9361 ggtgagccca aggtcacgga cttcgggatc gccagggcgg ccagctccga gacgctcacg
     9421 gttggctcga aggtgctcgg caccgccgcg tacctctccc cggagcaggc cagaggccgc
     9481 ccggtggacg cccggtgcga cctctacgcc atgggatgcg tcctctacga gatgctgacc
     9541 gcggcgccgc cgttccgcgg agggagcccg atctcggtgg ccaccaagca gatccacgag
     9601 gatccgcggc cgctgccgtc gtcggtcccg ccggcgctgg ccgccgtcgt gatgcaggcc
     9661 ctggagaagg acccggacga ccggttcccc gatgcgcggt cgatggccgc ggcgatccga
     9721 ggggccgcgg gcggggccgg ggagacgatg gtgctggagg agggcatgct cgagaccgag
     9781 gcgatcccgg cgccgcccgc ggcccctccg cgaaggtggg tgctcccggc gctcctcggc
     9841 gccctccttc tcgccgcgct gatcggcggg ctcgtgctgg ccttcgggcg ggacgacccg
     9901 accgccgagt ccaggggcgg ccgcgaccgc ggcggggcgg ccgcacagtc gtccccggcc
     9961 gagtcgccgc aggaggaggc cgagccgctc cgaccgcggg aagcgttcac ggccctcgtg
    10021 gggaccgtgc aggacgcgct cgcggccggg gagatcgagg agaagctggc gcgggacatc
    10081 gtcaagcggg ccgaggacgc gatggaagcc tacggtgagg ctgatctgca gaaggcccag
    10141 gacaaggccg gggatgcgat caacgcgctc gtggcgggcg ccgagcaggg ggaggtcacc
    10201 cccgagcgag cctccgagat cgagcagcgg atccggggcc tccaggccgg gttcgaggcc
    10261 tacccgccgc gcgagcccga ggaggaggac gagggcgggt cggacgacag gggcccggac
    10321 ggcgaggggc cccccgggcc cgacggcgaa ggccctcccg ggcagaacga caacacctag
    10381 ccggatgacc cccgccgcct cctcgacccg ataaagtctc accagatggc gaagcagcgg
    10441 gtcctcggca accgctacga gatcgagcgg gtcctcggcg aaggcggcat ggccaaggtg
    10501 ttcctcggga cggaccgggt cctggggcgc accgtggccg tcaaggtgct gtcgccccag
    10561 tacgccgagg accagcagtt cgtggcccgg ttccgccgcg aggcgcaggc agccgccgcc
    10621 ctcaaccacc ccaacatcgt gtcggtcttc gacacgggct ccaccggcaa cgtccactac
    10681 atcgtgatgg agtacgtgga ggggaggacc ctccaggaca ccctgcggga ggagagccgc
    10741 ctcacccccg agcgcagcat cgagatcgga gaggccgtag ccaaggccct ctccgcggcc
    10801 cacgccaagg gactcgtcca ccgtgacatc aagccgggca acatcatgat cacccgcgag
    10861 ggccaggtga aggtgatgga cttcggcatc gcccgggcca cgacctcgga cacggtgacc
    10921 caaacggccg cggtcctcgg gaccgccgcg tacctctccc ccgagcaggc ccagggcgag
    10981 accgtggacc accgctccga catctactcg atgggggtcg tgctgtacga gatgctcacg
    11041 ggccgccagc cgttcaccgg cgacagcccg gtctcgatcg cctaccgtca cgtcaccgag
    11101 gagccgatcc cgccctcccg gctcaacccc gacgtccggg agagcctcga cgcgatcgtc
    11161 atgaagtcga tggccaaggc gcccggcgat cgctatcaga gcgcgcagga gctcatcgac
    11221 gacctccgcc gcgcctccga ggggatgccc gtggccgcgg ccgttcccct ccccggcgac
    11281 acgacgcagg tgatcgaccg cggcggccac acccaggtcc tcaccggccc catcgaggat
    11341 gagatggagg aggaggagag aggccgccgg gtcgggtgga tcgtcttctg gaccctgctc
    11401 atcctggcga tcctcgggct ggcctccttc ttcctggttc gggcgctgct cagcggtccc
    11461 gagcccgtca cggtgcccga cgtggtgggc ttcaaccggg tggcggccgt ccagaccctg
    11521 gaggaggccg ggctcgaggt ggaggtcgtg cggagggctc gggacgacgt ccccgccgac
    11581 caggtcttcc gtcaggaccc cgaggaaggc accgaggtgg acgagggatc cgtcgttacg
    11641 atcttcgtca gccgcggggc ccgcatggtg gaggtcccaa gcgtggtcgg actctcccag
    11701 gaggcggccc agcaggcgat cgaggatgcc gggctcacgc tcggcttcgt ggggcaggag
    11761 ccgagcgacc aggacccggg catcgtcatc tcccaggatc cccagcccga gacggaggtc
    11821 gaggagggct cggccgtcaa catcgtggtc tccagcggcc ccgatacggt ggccgtcccc
    11881 agcgtggtgt gcgacccgcc cgaggacgcc cagagcgaga tcgagggccg ggacctcgtc
    11941 taccaggagg aggccgagga gttctcggac tgcccgtccg gcaccgtcgc ccgccaggac
    12001 ccggaggcag gcgccgaggt cgctccgggc tccacggttc gggtgtggat ctcgctgggc
    12061 ccctcgcccg agcccagccc cacggactcc ccgaccgatg gcgcctgaga cctcctgggt
    12121 cgaggagacc ttcaccgatg aggagcgaga gcgcctcgcg cccttcgtca cttcgctcga
    12181 agggcccgtg ttcgccctga tcaacctccc cgaggtgacg aagggcgccc tcttcgcccg
    12241 ctactcgcgg tcgtcgaagt ccgtccggcg gctgctgctc gacgagttcc tggaggaggg
    12301 cgagagcggg gccgcggccg aggaggccgg cgtgtcccgg gccgagcgcc tctacgagaa
    12361 cgtcttcttc gagtacgggg acgactcggt agcccagctc gggggcgtgc acctggcctg
    12421 cgagcagtcc tcgcagatcc tggccaaggt gctggagtgg gggcgcctcg cagcctacct
    12481 cgagcagtcc acccgatacg tgccctacga cgaccggccc ggcggccgct atcgctacgc
    12541 gatccctccc gaggtcgagg ccgcggggct ctcggcccgc tactccgagg tgctggaccg
    12601 cgcgttcgac acctacgcgc gttggctgga gccgatgcag gacctgtacc gggcccggca
    12661 cccccaagac cccggcgacc cggatttcgt ctaccgctcc acgatccggg ccaaggcctg
    12721 cgacaccctg cgcgggctgc tccccgcggc cacccggacc aacgtgggca tctacgccac
    12781 cggccaggcc tacgagcagc tcctgcttcg gatgcgggcg cacccttacg ccgaggtccg
    12841 ggcctacgcc gacctcatgc tgaccgagct gcggaaggtg atccccgcct tcctccggcg
    12901 cgtggacctc cccgaccggg gcggggcctg gtccgactac ctggcccgga ccagggagga
    12961 gacggccaag gtggccgaag agctggccgg cgacctccgg gcgcccccca gaccggaggc
    13021 cacgctgacc gacttcgacc ccgagggcga ggtgaaggtg gtggcggccg cgatgtatgc
    13081 ctccacgggc ctccccgacg acgtgctgct cgagcgagct cgggccatgt ccaccgagca
    13141 gcgggaccgg gtcctcgcag cctacgtggg cgaccgcccg aaccgtcggc acaagcccgg
    13201 ccgcgctttc gagcggaccg gctaccggtt cgacgtgctc tgcgactacg gggcgtttcg
    13261 cgaccttcag cgtcaccgga tgctcacgat cgagtggcag gagctgacga ccgcgcacgg
    13321 cttcgagcgg cctgacgacg tcgaggaagc gggcgcgcgt ccggactggg accgggtcat
    13381 ggaggagtcg gccgcgctcg agggcgagct ccgcgccgcg ggcctgggag gggtggccca
    13441 gtactctgtg gcgatggcct accggatccg cttcgtgatg cagatgaacg cccgcgaggc
    13501 gatgcacctg atcgagctcc gttcctcgcc gcagggacac cccgcgtacc ggcgggtggc
    13561 ccaccagatg caccggctga tacgggacga ggccggccac ggggccatcg ccggctccat
    13621 gcgacacctg gacctctccg aggtcgacct ggagcggctc gaggccgagc ggcgcgcggc
    13681 gtcccggcgg ggttcggagt gacgaagcag gactggctgt tcctggctct ggggacgctc
    13741 gccatcgcct cgctgctgac gtcgatcggg acgtggatgg ttctctggga ccggctgcgg
    13801 tcccgtcgct acgagtcctg agccgaggct ttctccttct cctcctcctg aagcttccta
    13861 cgcagcacct tgcccaccat cgtctcgggg agctcgtccc ggaactctac gtgcctgggc
    13921 gcccggtacc ccgtgaggcc gagctcggga tcgcgggccc aggtcttgat ctcgtcctcc
    13981 gtggcctcct ggccctcctt caggatcacg aaggccttca cggcctcccc cgtcttctcg
    14041 tcggggatcc ccaccaccga gaccttctgg accttcgggt gccggtagag gacctgctcc
    14101 acgtcggtgg ggtacacgtt gaacccgctg acgatgatga tgtccttctt ccggtctacg
    14161 atccggaagt agccgtccgg gtccatcttc gcgatgtccc ccgtgtggag ccaccggatt
    14221 ccgtcggggt ggacgcggat catctcctcg gtctcctccg gccggttcca gtagcccttc
    14281 atgacctggg gcccggcgat gcatagctcc ccctcgtcgt cgccgtcgac ctccctgtcg
    14341 gggtcgtcga tgtccacgag cttgcagtcg gtgtcgggga tgggaaggcc gatcgtcccg
    14401 aagcgatgtg agccgtccag cgggttgatg tgggtgacgg ggctcgtctc ggtgagcccg
    14461 tagccctcga cgagacgtcc gccggtgatc tgctcgaacc tctcggcgac cgccaccggg
    14521 agcggcgccg cgccggagaa gcaggagcgg atcgaggaga tgtcgtgctt cgccgtctcc
    14581 ggggactcgt tgatcgcgat gaagatccga ggaaccccgg ggaacagcgt ggggcgctcc
    14641 ttctccaccg ccttcagcac cagctccagc tcgaagcgcg gcaagagcac gagcttcagc
    14701 cccttgtaga tgccgacgcc catgatcgcg cccagcccgt aggagtggaa gaaggggagg
    14761 accgccacga tcccctcgtg gccctcctcg atccccgtga accacgccgc ggtctgagcg
    14821 gcgttcgcca cgatgttgaa gtgggtgagc atcgcgccct tggagagccc ggtcgtgccg
    14881 cccgtgtaga cgagcgcagc cacgtcctcc ttcgggtcga cctgggccgg cggcacgtcc
    14941 gggtagctgc ctttcatcag atcggcccac cacgtcacgt cggcgtcctg agggacgggc
    15001 ggccagcgac gcccctcgtg cttcgcctcc cgcctctgct tcaggggagc cagcatgttg
    15061 acggggaagc ccagatagtc cccgaccttc cccacgatca ccttccggaa ggcgacctcc
    15121 gtccgggccg cctccaccag cggatacagg agatccaggg cgatcagcac gtcgactccc
    15181 gcgtcgttca gctggtgcac gagctcccgc ttcgtgtaga gcgggttgtt tcccaccgtg
    15241 atcgccccga gacgctggat cgcgaagtgg gccaccacga agtgcgggga gttcgggagc
    15301 aggaggccca cccgatcacc gcgcttcacg ccgagcgacg ccagggcgcg cgccatctgc
    15361 tcgacctcgt ccagcagctg gcgataggtc agcgtcctcg acatcggggc gacggggaag
    15421 gccacggccg ggctgtccgg gaagcgctgg acggtgcgct ccagcatctc gaaggccgac
    15481 atctcagggt agggctccag tgtctggggg acgccctccg cgtagtggtc gagccagggg
    15541 cgagggcgct gcgtcctgtc gttcgccatc gggtcgcgca cctcctcgtc ggggagaccc
    15601 acatcattcc acagggcgct ccgggcgggc caccgggcct tgcccgggcg gcgcgggcgg
    15661 ccctagggtg accccgtgga cgactggatc gaggccctcc ggggggccgc ccgcgccgcc
    15721 ctcccgtccg tcgagggaga ggtccgcctc ccggggctga aggccgcggt cgagatccgg
    15781 cgggaccgct ggggcgtgcc tcacatctac gccgaccacc tgaccgacct ctggaccgcc
    15841 cagtcgtacg tgatggcctc cgagcgactc ttccagatcg agctggccta ccggctcggg
    15901 ctggggcggc tatcggagct gttcggggag ctcacgctct cgatggaccg gttcgtgagg
    15961 accctcggat ggaaccgtgc cgcggccgac cacgcggcga cctgggacga cctctcgctc
    16021 gagatgaccg cggccggcgc cgccggcgcc ctggcctggc tcgaggcgat gccggccagg
    16081 cccgtcgagt acgtcgtgct cggcgtcgac ccctggctcc ccgaaccgga agaggccgcg
    16141 ctcgccagcg cgggcgcggc catgtacgtc gcctggtctc tctcccgggc ctgggacaac
    16201 gacctcctgc gggccgagat cgccgagcgc ttcggggtcg aggcgatgcg cgcgctcttc
    16261 ccggacgccc ttcccgagcc cgaggccgtc cgcgcggcca aggaggccac cgatccacgg
    16321 ctggcgatgc tgcgggacgc gatcctcgcc ccctccggcg agggctcgaa cgcgtgggtc
    16381 gtggccggct cgcgttccgc gaccggtgcc ccgctgctgg cgaacgaccc ccacctcgtg
    16441 gtgcaggccc cgtccgcctg gttcgaggcg cacctggtcg ggccgggtgt cgacgtggcc
    16501 ggcgtgacgt tccccttcac gaccggcatc gtgatcggtc acaacgaccg gatcgcgtgg
    16561 gggttcacga actccgaggc ggacgtccag gacctgtatc tggagcggct gtccgaggac
    16621 ggggcctcga cgcagcacct cgacgcctgg gagcccgtga ccatggtccg ggaggagatc
    16681 ggggtgcggg ggcgggacga gccggaggtc ctcgaggtgc gcgagactcg ccacggcccc
    16741 ctcgtgggct cgtacatgat cgggatcgcc tccccccggg tgatccaggg acggatccga
    16801 cgaccgtacg ccctccggtg ggtgggagcg gagggcacga tcgagccctc cacgatccac
    16861 cggctcgaca ccgcgacctc ctgggagcag ttccgggcag ccgtcgcgcg ctggcactgt
    16921 ccgggccaga acatggtcta cgccgacgtc gaggggaaca tcggctacca gctcaccggt
    16981 cggtacccgg cgcggcggac gggcgacgga agcctccccg cgcccggctg gaccgaggag
    17041 cacgagtggg acgggtggat cccgttcgac gagctgccca gggccttcaa cccggaggcc
    17101 gggttcgtgg tgacggcgaa caaccgccct gatggcgaag gcggcccctt cctcggcgtc
    17161 gacttcctcc cgccctaccg cgcgcgccgc atcgcgcagc tggtgacggc tcgccctcgc
    17221 cacgatcgag agagcttcgc cgcgatccag accgacaccc gatcgctgct cgctccggag
    17281 atcctgcggc acctcctagc gatcgaaccc ggggacgagc ggcagaccga ggcgctcggg
    17341 ctgatggccg agtgggactg ggacctcggg cgcgactcgg ccccggccgc gatctgtgag
    17401 tcgtggtgca cccatctcgc cgcccggatc ctccgtccga ggctgggcag ggagctcttc
    17461 gagcactact tcgctcgccg ccagtggacg aacggcttcc agtaccgcgt cctccccgag
    17521 ctgctcgctt accccacggg gggctggttc ggcgaggatg gagccgccgg ccgcgacaag
    17581 gaggtcggcg ccgcgctcga cgaggcgctg accgaactca ccgatcggct gggcgagcac
    17641 atggcggact ggcgctgggg agccgtgcat cgagctcggt tcgcgggccg cctggccgcc
    17701 gtgcccgggc tcggcgagct cttcaccgtc ggcgaggtcg agctgggagg cgacgagcag
    17761 accgtggccc aagcccactt cgagcccggg gccccgtacg aagcggtcgt ggtccccagc
    17821 tggcggcaga tcgtcgatct gggggacctc gactcgtcgg tcggtgtgct caccctcggg
    17881 cagtcgggga accccgagag cccccactac cgggaccagc tcccgctgtg ggagtcggga
    17941 cagcaccacc cgatgccgtt cacgcgcgcc gccgtcgagc gggccacgac cacgaccctg
    18001 cgcgtgatcc cctagccctc gtcgagggcg atgcggacgc gcttgaacgc cttgcccttg
    18061 ctctcggtct tcgtcacgag tacccgaccc acctcaccgg tcgatcgcac gtgggtgccg
    18121 ccatcggcct gccggtcgat cccctcgatg tcgatcacgc ggatcgggtc caccgactcg
    18181 gggatgaggt tcaccttcgt ccggatcagg tcagggtccg cgagggcctc ggcccgcggg
    18241 aggaaccgca cgtggaccgg ccgatcctcg gccagggccg cgttcagctt ctcctcaacc
    18301 tcccggccga actcggcgct catctgctcg agttcgaagt ccatgcgggc ggtccccacg
    18361 tccatgttgc ccccggtgac ctttgcttcg tagtcgcgcc agatcaggcc cgacagggcg
    18421 tgcagggccg tgtggatgcg catcagccgg tggcggcgct cccagtcgat cgcggcggtg
    18481 acgaccgtcc ccggtgccgg ggcatcccct tcggccacat gcagcacccg gccgtcgcgc
    18541 ctgaccgtgt cgaccactcg gaccagtccc ccgtcccagc gcaggaggcc cgtgtcgccg
    18601 ggctggccgc ccccgcgagg atagaagacg gtccgatcga ggactactcc gtcctccccg
    18661 gatcccagga cggtggccgg ggtctctcgg gcgtacgcgt cggttgccgc gatctcctcg
    18721 gtcacgggcg aaggctagaa catcccgccg ccggggacga agcggctgac gtcgaggagg
    18781 tagaagaggg cgaacacgag gatcacggcg agagcgacga ggacgatcca gtagagcacc
    18841 gccagcacgg cgttgcgagg gctcagtcgg cggaagaagc cgatcatccg ggcgcctcca
    18901 gatccgacgg cgggtcgacc tgcacggccc ggacgatcag ccgcctcgcc gccgagaacc
    18961 gcggctcgca cgtggtcagc gtcagcgacg gtcgaggggt ccgatccagc acctccgact
    19021 ggctgggaag gacgacccgc gtccagagca cctcgtatgc gaactcgccc ctgttcgtcc
    19081 ggaggaagat ctcgtcgccc ggggcaagct cgttcagcga ccagaacggc ttgccgtagg
    19141 tggtccggtg tccggcgatc cccacgttgc cgccgtcacc cggcaagggg gtgcgggggt
    19201 agtgcccggg gcccctcttg aggtcctcga gcccgacccc ctcgaccacc acggcgtcga
    19261 ggtcgatgcg ggggatccga aggatggcca cccctctgcc gggaaccgga cgcaggtcct
    19321 ggttcgggct cgccacctgg aggcgtcttc cgaggtcctg gcggagtgcg tcctggtgcc
    19381 gcccggtgat gaggtcggtg acgaagagct cgtagacgac gaacagcgcc acgagggtgc
    19441 cggcggtgat cagcgtccag ccggcgccgc ggacgaggcg cgagccgagt ccgcggcgcc
    19501 ggcggccgga cctgggaggg gcttccaccg tcatgccgcc ggcgccgccg tcgccaactc
    19561 cctcaccccg cgccccacgg gcacgaccag ggagcctcgc tcgatctcga ggccgagacc
    19621 gaacgcgcgc acagcctcgt gcaggcgagg catccccggg ccctcctcca cggatcgctc
    19681 cagggcatcg ggatcgccga tcgcccggat ctcgtagggc gggctgtgga cgacgccgtg
    19741 cagcagcagc gtgttgcccg cacagcggac ggccgagagg ctcgacagcc gctcgccgtt
    19801 gatggcgacg gcctccgcgc cggcggccca cagcgcgttc accacggtct ggatgtcccg
    19861 ctcgtggacg ataaggtcgt tgggatcgcc ggtccccgag cgggaggccg aggagtcgtc
    19921 gaggacgacc gtgatccccg ggccgcggag cggcgtggcg ccggccccca ccgccagcgc
    19981 ctccgcctcg gcccggagct ccccgatcgc ggcgctcgtc tcgccaccgc ccgcctcccg
    20041 gatccgagcc tcgagctccg ccacctcctc gcgcagctcc cgaacccgcc cgtcctgctg
    20101 ctcgatcagg tcgacgatct cgaggcgcga ggactcgcgc cccggaaccg cctgaggctg
    20161 ggccgccgcc agcaccgcga ggaaccccag gaccccggca gccaggggca tcgcgaagcg
    20221 ggagccgggc cgatccatgc ggctatgata ggtgccgtcc cgacggaaag gagccgggtg
    20281 cccaagtcga agtcgaagcg ccgcagctac cagcctccca agccgcccaa gccgaagccc
    20341 agccccacct ggttcgggat cctcgtcctg acactcctgt tcgtcggggt cctgatgatc
    20401 gtgctgaact acctgggctt cctgctcccc gggtcgccga acaacaccta cctctgggtc
    20461 gggctcggcc tgatcgccgt gggcttcgcc ttggccacgc agtggcgctg acctggtctt
    20521 tcgtgcgccc atggggcgtt ttccacactg cttctccaca ttgtggaacg aatcacatgg
    20581 acgtgatttg atgcccgtct acgagttccg ctgcgaggac tgcggcgcgg cgctcgagct
    20641 ggtgcgcccg atggggcgga ccgagccccc cggctcctgc cccgcctgcg ggggctcgcg
    20701 gatgcgccgc cgctggagcc gcgtgggcgt ccgcttcgag ggatggggct tctccaggac
    20761 cgacgccctg ctccccgagg accggcgcgc ccggcgcgac ttcaagcagc tgaagcgcag
    20821 ggccgacgag atcgcggagt cttagcggag cggcgccggc cgctgctcga cgcgcatggt
    20881 ctctccgcag tgccggggca cctggagctc gccgagcttc tcgacgcgga actccgttcc
    20941 gcactcgccg cagacgaagt agacgtcgag ggcctcgacg tcctcgacgg gggcctcccg
    21001 gacgaggtcg cggttgcggc cgatcgccga caggatccag atcccgaggc cggcgacccc
    21061 caccgcgagc aggacggcga acgcgaaccg cacgacgtca gtcaaccacg aggcgctcga
    21121 tgatggtggc gttggccagg ccaccgccct cgcacatcac ctggagcccg taccggccct
    21181 cacgccgctc gagctcgtgc agcatcgtgg tcatcaggcg gccgcccgag gccccaagcg
    21241 ggtgccccag cgcgatcgcc ccgccgttcg ggttcacgtg ggcctcgaac cagtcgtcgg
    21301 ggatctcgag ctcccgcttc cacgccagca ccaccgaggc gaaggcctcg ttgatctcca
    21361 agacgtcgac gtcctcgagc gagagccccc cggacgcgag cactttccgc gtggcctcga
    21421 tcggcccggt caacatcgtg atcgggtcca cccccgacag cgcgaaggcc tggaaccggg
    21481 cccgcggccg cagcccaagc tcgtcggcct tctcccggga ggcgatcacg accgccgcgg
    21541 ccccgtccga gatctgcgag gagttccccg ccgtcaccac gccgtcctcc ttgaaggggg
    21601 tcggaagcgt ggccatcttc tccaggtcca cctcgaatcg gaccccttcg tcgtgctcga
    21661 agagctcggt ctccctctcg gtgccgagct cgatcggatg gatctcgtcc tggaagcgac
    21721 cgtccttgat ggccttggcg gcgagctcgt ggctgcgggc gctgaactcg tcgagctccc
    21781 cccgcgagag gttccagcgc tcggcgatga tctccgcgga gacgccctgc ggcacgagac
    21841 ggccgtcgta gcgatcgagc agccgctggc tgaacgggaa ccccggtccc tgctccatcg
    21901 tcacgcccat ggggacgcgg gtcatgtgct cgatccccga ggcgatcgcg aggtcgtaag
    21961 cgcccgccat cacgccctgg gccgcgaagt gcgccgcctg ctgggaggag ccgcactgac
    22021 ggtcgaccgt gaccgaaggc acctcctcgg ggaggcccgc ggccagccag gcgttccgcc
    22081 cgatgttgat cgcctgctcc cccgtctgcg agacacagcc ggtgatgttg tcgtcgaccc
    22141 gggcggggtc cacgcccgtg cgctccacgg ccgcgctcag cacctcggcc aggaggtcca
    22201 cggggtggga gcccgagagc ttcccgcctc ggcgcccgac gggcgatcgg acggcctcga
    22261 cgatcacggc ttcgcgcatg ggaacctcct ggcccggtta cctactcgtg agtaacctac
    22321 cctaccctcg gggccgcaca tcgtcaatcg ccccgaccct gtgcggggaa tgcgtgtatc
    22381 ctctgccgtc cgcctgaaca agggagggtc agcatgagga cggttctggg acgggggagc
    22441 gcgacgggcg cggccctggc ggcggtggcc gtggcgctct cgctcaccac cacggtgctg
    22501 gcgacgccga cgacgatcaa cgacgtcgat ctcaccgcgg gtggaggggc ggtgttcgat
    22561 cccgagcacg agacctacgg ctgcgtcttc gacgaggaca cccggtacta ctcgcccatc
    22621 ggcgacggct cgatcgggat ccgcaccgac gggttcgacg gcgccttggt tctgtgggtg
    22681 ggcgacaacg cgttcaccga cgccgacggc gtgggcaacc tcgtcggtca gcagctgacc
    22741 gtcggcccca ccaaggtctc cggggtcaag gtgacgcgga tcgagcgggc cctccccggg
    22801 agccggaccc tgcgttcgct catcaagctg accaatccct cgaagaagat cctgcgtcgc
    22861 agcatcacgc tggacacgaa cctgggatcc gacggcagca ccgcgctcga gctctccagc
    22921 agcggggaca tccgcttcga cagtcgggac cgctggggca tcacgagcga gcagcccggg
    22981 agcgaccccg tcgtgacgat ggtcaactac gggaagggaa gggtcgtccg ccctcagttc
    23041 cagatcggtc cggtggccgg cgacgagtgc atcctcacgt cgttccggat caccctcggt
    23101 ccgaagcaga cccggtacat gatgttcttc gtcgagctgt ccgaggacga cctggacgcc
    23161 gcctcgagcg cgaggaagtt caacaggaag cggctcagca gcgccctcct ggcgggcctc
    23221 ggctcggagg tgcggcagcg catcctcgac tgggatctga cgtaagggag gggccccctg
    23281 ccgatcgaga tc
//
